From eva.alstromer <@t> histolab.se Tue Jan 2 06:12:31 2007 From: eva.alstromer <@t> histolab.se (=?iso-8859-1?Q?Eva_Alstr=F6mer?=) Date: Tue Jan 2 06:12:47 2007 Subject: [Histonet] unsubscribe In-Reply-To: Message-ID: <000c01c72e67$47b80560$65b6a8c0@HISSWE.lokal> Eva Alstr?mer Histolab Products AB S?dra L?ngebergsgatan 34 S-421 32 V Fr?lunda Sweden +46 (31) 7093032 eva.alstromer@histolab.se www.histolab.se -----Ursprungligt meddelande----- Fr?n: HistoNet Server [mailto:HistoNet@Pathology.swmed.edu] Skickat: den 24 september 1998 22:02 Till: Eva_Alstr#154#mer ?mne: re: subscribe Your address has been added to the addresses that comprise this Listserv List. Welcome to HISTONET. This is an electronic mailing list for the exchange of information pertaining to histotechnology and related fields. PLEASE SAVE THIS MESSAGE. It contains useful information about how to use the list and what to do if you experience problems. It also includes some basic rules for email etiquette (Netiquette) which will be helpful to those who are new to this form of communication. WHAT IS A LISTSERVER? A list server is a computer that runs software which will receive incoming electronic mail (email) messages and reroute them automatically to everyone on the subscriber list. Email uses the vast expanse of the Internet to allow almost instantaneous communication between networked computers around the world. Our system uses the LISTSTAR software from Quarterdeck Corporation (California) and can currently send about 30 messages a minute. With the present number of subscribers, we are processing about 10,000 outbound messages a day. WHO SHOULD SUBSCRIBE? Anyone interested in research or clinical applications of histology, immunohistochemistry, in-situ hybridization pathology, and electron microscopy may find Histonet informative and useful. Currently, there are more than 850 subscribers from all over the world. Subscribers include hospital employees from major urban centers and obscure remote locales, university researchers, botanists and the employees of commercial laboratories, government agencies, veterinary facilities and a wide variety of commercial industrial ventures. WHO RUNS HISTONET? The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using hardware and software owned by the University of Texas Southwestern Medical School, Department of Pathology in Dallas, Texas. If you have any questions or problems with Histonet please contact Linda Margraf at LMargraf@childmed.dallas.tx.us. HOW DOES THE LIST WORK? This server, unlike many systems, uses ONLY ONE ADDRESS to send commands to the computer and to post messages. The server will recognize commands sent in the SUBJECT line of the message and only when they are spelled exactly as listed below. Anything not identified as a command will be circulated to EVERYONE on the list. The following is a list of commands the server recognizes: subscribe Your address will be added to the list of subscribers. You will then be able to send messages to this list that will be forwarded to all other list subscribers. You will begin to receive all messages sent to the list by other subscribers. subscribe digest Your address will be added to the list of subscribers who receive a digest instead of each forwarded message. A digest is a compilation of all the messages received in a 24 hour period. It is sent to the digest subscribers every night after midnight. Digest subscribers can post and respond to messages the same as "real-time" subscribers. digests A list of available digests will be returned to you. Histonet stores old messages as daily digests for approximately three months. To read previous messages, copy the list of available digests, mark the dates of interest and return it to the server. unsubscribe Your address will be removed from the list of subscribers. You will no longer be able to send messages to the members of the list. help A list of the commands recognized by the server will be returned to you. WHAT ARE THE RULES? You may post any questions you wish pertaining to histology, pathology, in-situ hybridization, immunohistochemistry etc. Equipment and reagent evaluations, laboratory management issues, government regulations, and job opportunities are all appropriate topics. The University asks that we restrict the use of its hardware and software to business purposes only (occasional jokes do slip through but PLEASE use restraint). Vendors and those with commercial interests in histology products are welcome contributors however, we ask that blatant advertisements be avoided at all times. It is fine to refer to product that your company produces if it is pertinent to a topic being discussed on the list. Unsolicited advertisements are poorly tolerated by the members and you will likely receive a number of negative comments if you overstep the boundaries. Please contact Linda Margraf at LMargraf@childmed.dallas.tx.us if you are not sure about the appropriateness if a message you wish to post. BASIC HISTONET "NETIQUETTE" It is most helpful to the list members if you post your responses to queries to everyone on the list and not just as a personal reply to the person asking the question. That way duplicate messages are minimized and we all learn from each other's comments. Likewise, if you post a question and get a number of responses back directly to you, it is helpful to everyone if you could send out a summary of the replies you got to Histonet. Please avoid abbreviations unless they are explained in your message. For example: immunohistochemistry (IHC). This list circulates to a wide variety of individuals and what seems obvious to you may have no meaning on the other side of the world. Please sign your letter and include your institution or affiliation and location. Not all email systems have headers which identify the sender. Do use the subject line to indicate the topic of your message. DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. Please send questions and problems about the list directly to Linda Margraf at LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 subscribers on the list. Be careful when sending commands to the server to put the command in the SUBJECT LINE and spell it correctly. Please do not send images as attachments with your message. We can now post images at our web site (http://pathcuricl.swmed.edu). To have an image posted send it to Herb Hagler at herb.hagler@email.swmed.edu. es From Jackie.O'Connor <@t> abbott.com Tue Jan 2 11:53:31 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jan 2 11:54:07 2007 Subject: [Histonet] STF replacement In-Reply-To: <1D71A10BB247204A9EFFB9EED323605801916B97@XCH-VN02.sph.ad.jhsph.edu> Message-ID: OK - just back from an extended leave. I called STRECK Laboratories, and they did in fact confirm that they are no longer offering STF for sale. I also would appreciate any suggestions from anyone who routinely uses a formalin substitute for IHC, particularly on mouse tissue. Thanks. Jackie O'Connor "Hannah, Michele F." Sent by: histonet-bounces@lists.utsouthwestern.edu 12/14/2006 03:31 PM To cc Subject [Histonet] STF replacement I just called to check on an order of STF that I had placed and was told that it has been discontinued with no replacement. Just wondering if anyone else has found a replacement or knows of one. We use it exclusively for postfixation. Thanks! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Tue Jan 2 12:03:55 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue Jan 2 12:03:48 2007 Subject: [Histonet] IHC QC position open at Lab Vision Message-ID: Here is an open position at Lab Vision Corp (part of ThermoFisher Scientific). Please send resumes to me at the emial address below. Please note that preference will be given to local candidates (California). ************************************************************************ ************************************** Quality Control Research Associate II Summary Performs quality control tests on antibodies, detection systems, and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. May oversee work of QC Research Associate I. Major Responsibilities Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs product IHC stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with Lab Vision/NeoMarkers' Quality Policies and Quality Procedures. Able to work closely with other departments in reaching company goals. Education and/or certification requirements BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. Certification as Histotechnologist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Reponsibilities May oversee work of QC Research Associate I. Lab Vision, part of ThermoFisher Scientific, manufactures antibodies and automated immunohistochemistry staining platforms for the clinical and research histology laboratory. Lab Vision is located in Fremont, California in the heart of the San Francisco Bay area biotechnology industry. ************************************************************************ *************************** Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 tpmorken@labvision.com or Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From nena.dimaano <@t> roche.com Tue Jan 2 12:13:20 2007 From: nena.dimaano <@t> roche.com (Dimaano, Nena) Date: Tue Jan 2 12:13:36 2007 Subject: [Histonet] Embedding Weights Message-ID: <5034B118F526E547A9B897721075839305DCC5A6@rnumsem02.nala.roche.com> Happy New Year Everyone, Does anyone know where to get the Embedding Weights? We tried Fisher, VWR and EMS but we were told that they don't have them. Thanks, Nena Nena Dimaano Hoffmann La Roche 340 Kingsland Ave. Nutley, NJ 07110 tel: 973-235-3953 fax: 973-235-4710 email: Nena.Dimaano@roche.com From ahacking <@t> yahoo.com Tue Jan 2 12:14:26 2007 From: ahacking <@t> yahoo.com (Dr. Adam Hacking, PhD) Date: Tue Jan 2 12:14:33 2007 Subject: [Histonet] maximize the hardness of MMA Message-ID: <20070102181426.18628.qmail@web30803.mail.mud.yahoo.com> Hi, Does anyone know how to increase or maximize the hardness of MMA? Are there harder embedding materials ? Many thanks Adam Hacking. PhD ________________________________________________________________ Dr. S Adam Hacking, Post Doctoral Fellow JTN Wong Laboratories for Mineralized Tissue Research, Center for Bone and Periodontal Research, McGill University 740 Dr. Penfield Ave. Rm. 2201 Montreal, QC, Canada H3A 1A4 Ph.: 514-398-5112 Fax: 514-398-4020 From tbraud <@t> holyredeemer.com Tue Jan 2 12:16:26 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 2 12:18:22 2007 Subject: [Histonet] STF replacement In-Reply-To: Message-ID: What an eye-opener! I come from a lab where a formalin substitute was used for almost 10 years, then had it discontinued; then found an alternate source, only to have it discontinued after 5 more years. Forget trying to make it yourself because the ingredients were proprietary. Then, there was the need for all those pesky "alternative" NBF protocols for staining and IHC, especially the CAP surveys, which were always Neutral Buffered Formalin Fixed. Certainly there are good, safer substitutes available, but none have the history of formalin and none are approved as a fixative for any of the FDA approved IHC tests. Best of Luck to the Histotechs who have used this product for years, optimized all of their procedures for it, only to be left hanging. Terri Braud -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M O'Connor Sent: Tuesday, January 02, 2007 12:54 PM To: Hannah, Michele F. Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] STF replacement OK - just back from an extended leave. I called STRECK Laboratories, and they did in fact confirm that they are no longer offering STF for sale. I also would appreciate any suggestions from anyone who routinely uses a formalin substitute for IHC, particularly on mouse tissue. Thanks. Jackie O'Connor "Hannah, Michele F." Sent by: histonet-bounces@lists.utsouthwestern.edu 12/14/2006 03:31 PM To cc Subject [Histonet] STF replacement I just called to check on an order of STF that I had placed and was told that it has been discontinued with no replacement. Just wondering if anyone else has found a replacement or knows of one. We use it exclusively for postfixation. Thanks! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From cgfields <@t> lexhealth.org Tue Jan 2 12:19:06 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Tue Jan 2 12:19:17 2007 Subject: FW: [Histonet] Embedding Weights Message-ID: -----Original Message----- From: Carole Fields Sent: Tuesday, January 02, 2007 1:19 PM To: 'Dimaano, Nena' Subject: RE: [Histonet] Embedding Weights They are called Tampers and Cardinal sells them in the section under Tissue-Tek products. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Dimaano, Nena [mailto:nena.dimaano@roche.com] Sent: Tuesday, January 02, 2007 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Weights Happy New Year Everyone, Does anyone know where to get the Embedding Weights? We tried Fisher, VWR and EMS but we were told that they don't have them. Thanks, Nena Nena Dimaano Hoffmann La Roche 340 Kingsland Ave. Nutley, NJ 07110 tel: 973-235-3953 fax: 973-235-4710 email: Nena.Dimaano@roche.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From RSRICHMOND <@t> aol.com Tue Jan 2 12:39:11 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Jan 2 12:39:27 2007 Subject: [Histonet] Re: STF replacement Message-ID: Ten years ago on Histonet Hal Hawkins posted: >>We have been very pleased with the results of immunostaining tissues fixed with Streck Tissue Fixative (Streck Labs Inc., PO 37625, Omaha, NE 68137-0625, (402) 691-7453). It contains Bronopol, which is 2-bromo-2-nitro-propanediol, and diazolidinyl urea, C7H14N4O7, with zinc sulfate and sodium citrate, and less than 0.1% formaldehyde. It meets government standards for disposal in public sewers, as not forming hazardous vapors, and for shipping as a nontoxic substance. Prolonged fixation does not mask antigens. I presume it is a weak cross-linker and denaturant. The morphology is excellent at the light level, but no good by EM.<< Checking my "Funny Fixatives" file, I don't find any similar fixative. I think that STF may have functioned as a transport medium, so that fixation would occur in the processor. Ethanol would thus be the fixative unless the processor sequence began with formaldehyde. A good lesson in why you should use laboratory materials of known composition whenever possible, rather than relying on mysterious proprietary mixtures. Bob Richmond Samurai Pathologist Knoxville TN From lblazek <@t> digestivespecialists.com Tue Jan 2 12:47:01 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jan 2 12:45:20 2007 Subject: [Histonet] Embedding Weights Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684C80@bruexchange.digestivespecialists.com> Do you mean those little smashy things? If that is what you are looking for Fisher has them and they are called Tampers. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dimaano, Nena Sent: Tuesday, January 02, 2007 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Weights Happy New Year Everyone, Does anyone know where to get the Embedding Weights? We tried Fisher, VWR and EMS but we were told that they don't have them. Thanks, Nena Nena Dimaano Hoffmann La Roche 340 Kingsland Ave. Nutley, NJ 07110 tel: 973-235-3953 fax: 973-235-4710 email: Nena.Dimaano@roche.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Tue Jan 2 13:05:50 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jan 2 13:06:01 2007 Subject: [Histonet] Slide Dryer Message-ID: <000801c72ea1$04ea3a20$db01a8c0@plab.local> I am in search of a used slide dryer, or incubator to dry my IHC slides. I don't really need new for our work load and its difficult for the one oven I do have to accommodate both Immuno and H&E. Anyone have a dust-off stored in the basement?? LET"S MAKE DEAL! Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne From pmarcum <@t> vet.upenn.edu Tue Jan 2 13:21:57 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Jan 2 13:22:05 2007 Subject: [Histonet] STF replacement In-Reply-To: References: Message-ID: <6.2.5.6.2.20070102141546.01c6f8d8@vet.upenn.edu> Terri is correct and with the FDA approved IHC tests using a mixture of fixatives other than or with NBF is not an option anymore. These tests require NBF for fixation at all phases. You can't do pre-fix in NBF and then switch to a substitute on the processor or for storage or vice versa . I think of it as not doing the patient any good by calling the test results into question with a non-recommended fixative on these tests. Until the companies with FDA approved IHC tests are willing to test all substitutes and tell you which ones you use (id they are still on the market) for which test we will need to use NBF. Sorry. Pam Marcum At 01:16 PM 1/2/2007, Terri Braud wrote: >What an eye-opener! > >I come from a lab where a formalin substitute was used for almost 10 >years, then had it discontinued; then found an alternate source, >only to have it discontinued after 5 more years. >Forget trying to make it yourself because the ingredients were proprietary. >Then, there was the need for all those pesky "alternative" NBF >protocols for staining and IHC, especially the CAP surveys, which >were always Neutral Buffered Formalin Fixed. >Certainly there are good, safer substitutes available, but none have >the history of formalin and none are approved as a fixative for any >of the FDA approved IHC tests. >Best of Luck to the Histotechs who have used this product for years, >optimized all of their procedures for it, only to be left hanging. > >Terri Braud > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M >O'Connor >Sent: Tuesday, January 02, 2007 12:54 PM >To: Hannah, Michele F. >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: Re: [Histonet] STF replacement > > >OK - just back from an extended leave. I called STRECK Laboratories, and >they did in fact confirm that they are no longer offering STF for sale. I >also would appreciate any suggestions from anyone who routinely uses a >formalin substitute for IHC, particularly on mouse tissue. >Thanks. > >Jackie O'Connor > > > >"Hannah, Michele F." >Sent by: histonet-bounces@lists.utsouthwestern.edu >12/14/2006 03:31 PM > >To > >cc > >Subject >[Histonet] STF replacement > > > > > > >I just called to check on an order of STF that I had placed and was told >that it has been discontinued with no replacement. Just wondering if >anyone else has found a replacement or knows of one. We use it >exclusively for postfixation. Thanks! > > >Michele > >Michele F. Hannah M.S. >Department of Molecular Microbiology & Immunology >Johns Hopkins Bloomberg School of Public Health > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to which >it is addressed. It may contain information that is privileged >and/or confidential, >and the use or disclosure of such information may also be restricted >under applicable >federal and state law. If you received this communication in error, >please do not >distribute any part of it or retain any copies, and delete the >original E-Mail. >Please notify the sender of any error by E-Mail at the electronic >address shown. > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From kbowden <@t> ucsd.edu Tue Jan 2 13:32:31 2007 From: kbowden <@t> ucsd.edu (K. Bowden) Date: Tue Jan 2 13:32:52 2007 Subject: [Histonet] maximize the hardness of MMA In-Reply-To: <20070102181426.18628.qmail@web30803.mail.mud.yahoo.com> References: <20070102181426.18628.qmail@web30803.mail.mud.yahoo.com> Message-ID: <459AB34F.6010105@ucsd.edu> You can control the hardness of MMA with the amount of n-butyl phthalate. If you don't add phthalate your MMA will be as hard as possible. Karen Bowden University of CA, San Diego Dept of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 Ph.: 858-534-4655 Fax: 858-534-5304 Dr. Adam Hacking, PhD wrote: > Hi, > > Does anyone know how to increase or maximize the hardness of MMA? > > Are there harder embedding materials ? > > Many thanks > > Adam Hacking. PhD > > > ________________________________________________________________ Dr. S Adam Hacking, Post Doctoral Fellow > JTN Wong Laboratories for Mineralized Tissue Research, > Center for Bone and Periodontal Research, > McGill University 740 Dr. Penfield Ave. Rm. 2201 > Montreal, QC, Canada H3A 1A4 > Ph.: 514-398-5112 > Fax: 514-398-4020 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From histology <@t> gradymem.org Tue Jan 2 13:36:17 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue Jan 2 13:41:09 2007 Subject: [Histonet] Embedding Weights In-Reply-To: <5034B118F526E547A9B897721075839305DCC5A6@rnumsem02.nala.roche.com> References: <5034B118F526E547A9B897721075839305DCC5A6@rnumsem02.nala.roche.com> Message-ID: We had to replace a lost one lately. We ordered ours from Sarkura (Tissue Tek)--it is listed in the manual as a replacement part (small and large tampers). Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Dimaano, Nena" Date: Tuesday, January 2, 2007 1:17 pm Subject: [Histonet] Embedding Weights To: histonet@lists.utsouthwestern.edu > > Happy New Year Everyone, > Does anyone know where to get the Embedding Weights? We tried Fisher, > VWR and EMS but we were told that they don't have them. > > > Thanks, > Nena > > > Nena Dimaano > Hoffmann La Roche > 340 Kingsland Ave. > Nutley, NJ 07110 > tel: 973-235-3953 > fax: 973-235-4710 > email: Nena.Dimaano@roche.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pmcardle <@t> ebsciences.com Tue Jan 2 13:58:05 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Tue Jan 2 13:58:19 2007 Subject: [Histonet] This is a posting from a vendor Message-ID: <459AB94D.6060704@ebsciences.com> Hello: Up front, be aware, this message is from a vendor, and I know how many of you feel about vendors posting on the Histonet. The nature of this posting is as non-commercial as possible given the source, but feel free to disregard or flame as you see fit :-) Energy Beam Sciences was one of the pioneers in microwaves for histological applications, and our alliances with clinical and research professionals have resulted in many advances over the years. To this end, I felt the Histonet would be a good forum to try and line up beta-testers for some of our newly-developed microwave histology products. (Actually, beta testing has already been performed with some of these products, but rigorous testing and/or a wide array of independent opinions are always warranted when clinical diagnostics may be involved.) Some specifics: (a) a method (patent pending) for heating paraffin in the microwave (b) an improved reagent for microwave processing (c) several microwave accessories I want to stress that this is not a cheesy attempt to drum up business; we anticipate at least one of these products may result in a methods paper that could have significant impact, so any input would be welcome. Beta-testers could be with a hospital, independent clinical lab, University, or possibly another (non-competing) vendor; we are looking for as wide a variety of workflows and disciplines as possible. Please feel free to contact me at pmcardle@ebsciences.com with any questions. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi From gu.lang <@t> gmx.at Tue Jan 2 14:24:01 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 2 14:21:49 2007 Subject: [Histonet] dehydration after staining Message-ID: <000f01c72eab$f10e73d0$c812a8c0@dielangs.at> Hi all, Is it possible to over-dehydrate the slides after staining? We do our regular HE-stain with increasing ethanol-concentrations for 1-2 min and then clear in butyl-acetat before coverslipping with Pertex and glass-coverslips. One of our pathologists complains about an glassy-3D-appearence of the tissue, especially of collagenfibers and mamma. The effect is most visible in his microscope, which is the newest (2002), but only with the 4er and 10er objective. When he switches the condensor in, the effect is gone, but there is only the small bright space. He compared it to older slides (cleared in Xylol or Limonen, coverslipped with film or glass) and saw the same effect in a milder way. The other pathologists don't see the same with their (older) microskopes. Could this be a matter of the microscope or a matter of clearing? Or a matter of wrong handling of the microscope? I would be happy for any input. Gudrun Lang From mcauliff <@t> umdnj.edu Tue Jan 2 14:40:45 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jan 2 14:39:52 2007 Subject: [Histonet] dehydration after staining In-Reply-To: <000f01c72eab$f10e73d0$c812a8c0@dielangs.at> References: <000f01c72eab$f10e73d0$c812a8c0@dielangs.at> Message-ID: <459AC34D.5010906@umdnj.edu> Sounds like a poorly aligned (or dirty) microscope to me. Who looks at slides with the conderser out? (answer: people who don't understand microscope optics). I don't see how one can "over-dehydrate" a slide. If you leave some water in it will form bubbles as it separates from the mounting media. Good luck! Geoff Gudrun Lang wrote: >Hi all, > >Is it possible to over-dehydrate the slides after staining? > > > >We do our regular HE-stain with increasing ethanol-concentrations for 1-2 >min and then clear in butyl-acetat before coverslipping with Pertex and >glass-coverslips. > >One of our pathologists complains about an glassy-3D-appearence of the >tissue, especially of collagenfibers and mamma. The effect is most visible >in his microscope, which is the newest (2002), but only with the 4er and >10er objective. When he switches the condensor in, the effect is gone, but >there is only the small bright space. He compared it to older slides >(cleared in Xylol or Limonen, coverslipped with film or glass) and saw the >same effect in a milder way. The other pathologists don't see the same with >their (older) microskopes. > >Could this be a matter of the microscope or a matter of clearing? Or a >matter of wrong handling of the microscope? > > > >I would be happy for any input. > > > >Gudrun Lang > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gu.lang <@t> gmx.at Tue Jan 2 15:02:30 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 2 15:00:18 2007 Subject: AW: [Histonet] dehydration after staining In-Reply-To: Message-ID: <001001c72eb1$51972ee0$c812a8c0@dielangs.at> Unfortunatly I am not a microscope expert. But last time he tried, to open the condensor (I think) and the effect was milder. But it wasn't optimal. Something like making the microscope worse than it actually is. I will try do get the answers to your questions (diffuser,...) and then come again. Gudrun Lang From ASenn <@t> mercy.pmhs.org Tue Jan 2 15:02:40 2007 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Tue Jan 2 15:02:51 2007 Subject: [Histonet] Slides Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A83@pmhs2kxch03> My 7 year old son got a microscope for Christmas. He's very excited to look at slides! The microscope came with 3 slides: Pine needle, onion peel, and pumpkin stem. Problem is, he's bored with those slides and wants more. I made him a few here in the surgical path dept. on the cryostat, but I'm not directly in histology anymore, so I can't just cut a few extras and stain them myself. I don't know where to get more slides for him, short of paying 29.99 PLUS shipping and handling for only TEN slides from the place where I got the microscope to begin with. Anyone have some extras they'd like to share? He'd really like to see some colors and 'funky designs' as he likes to say. We made a few from his spit and even one from his brother's boogers (lol) but he's getting tired of the same old stuff & is acutally really interested in what the 'funky colors' mean.... I'd be willing to pay postage..... Thanks so much for any help!! Amy From rjbuesa <@t> yahoo.com Tue Jan 2 15:02:57 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 2 15:03:05 2007 Subject: [Histonet] dehydration after staining In-Reply-To: <000f01c72eab$f10e73d0$c812a8c0@dielangs.at> Message-ID: <661614.77046.qm@web61222.mail.yahoo.com> Gudrun: You cannot overdehydrate, you either dehydrate completely or do not dehydrate completely. Many (many) years ago the Zeiss microscopes used to have in their Abb? condenser the ability to slide sidewise their field diafragm, and by doing so you were able to obtain "obliquous" illumination = some 3D effect. To me the problem is of very different difractive index between the dehydrated tissue and the clearing agent, perhaps enhanced by a=some missalignment of the pathologist's microscope. It seems to be an optical artifact. I think that the pathologist shoudl try to use field (Koehler) illumination and determine if the effect continues. I think he is using too much light and a too close diafragm. Ren? J. Gudrun Lang wrote: Hi all, Is it possible to over-dehydrate the slides after staining? We do our regular HE-stain with increasing ethanol-concentrations for 1-2 min and then clear in butyl-acetat before coverslipping with Pertex and glass-coverslips. One of our pathologists complains about an glassy-3D-appearence of the tissue, especially of collagenfibers and mamma. The effect is most visible in his microscope, which is the newest (2002), but only with the 4er and 10er objective. When he switches the condensor in, the effect is gone, but there is only the small bright space. He compared it to older slides (cleared in Xylol or Limonen, coverslipped with film or glass) and saw the same effect in a milder way. The other pathologists don't see the same with their (older) microskopes. Could this be a matter of the microscope or a matter of clearing? Or a matter of wrong handling of the microscope? I would be happy for any input. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bhewlett <@t> cogeco.ca Tue Jan 2 15:15:54 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Jan 2 15:16:06 2007 Subject: [Histonet] Slides References: <81C95EFFB67F284B9FC080B91954F81DD61A83@pmhs2kxch03> Message-ID: <006701c72eb3$31464160$6500a8c0@mainbox> Amy, If its available in your area, get him to look at pond water, full of neat stuff!! An alternative is to put some hay and rainwater in a large jar and wait for a few days. It will grow lots of neat plants and animals which change over time. Regards, Bryan ----- Original Message ----- From: "Senn, Amy" To: Sent: Tuesday, January 02, 2007 4:02 PM Subject: [Histonet] Slides > My 7 year old son got a microscope for Christmas. He's very excited to > look > > at slides! > The microscope came with 3 slides: Pine needle, onion peel, and > pumpkin > > stem. > Problem is, he's bored with those slides and wants more. > I made him a few here in the surgical path dept. on the cryostat, but > I'm > not directly in histology anymore, so I can't just cut a few extras and > stain them myself. > I don't know where to get more slides for him, short of paying 29.99 > PLUS > shipping and handling for only TEN slides from the place where I got > the > > microscope to begin with. > Anyone have some extras they'd like to share? He'd really like to see > some > colors and 'funky designs' as he likes to say. We made a few from > his > > spit and even one from his brother's boogers (lol) but he's getting > tired of > the same old stuff & is acutally really interested in what the 'funky > colors' mean.... > I'd be willing to pay postage..... > Thanks so much for any help!! > Amy > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nena.dimaano <@t> roche.com Tue Jan 2 15:18:44 2007 From: nena.dimaano <@t> roche.com (Dimaano, Nena) Date: Tue Jan 2 15:19:07 2007 Subject: [Histonet] Embedding Weights In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684C80@bruexchange.digestivespecialists.com> Message-ID: <5034B118F526E547A9B897721075839305DCC5A7@rnumsem02.nala.roche.com> Thanks to all who quickly replied to my embedding weights inquiry. Best Regards, Nena -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Tuesday, January 02, 2007 1:47 PM To: Dimaano, Nena {NCDS~Nutley}; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Embedding Weights Do you mean those little smashy things? If that is what you are looking for Fisher has them and they are called Tampers. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dimaano, Nena Sent: Tuesday, January 02, 2007 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Weights Happy New Year Everyone, Does anyone know where to get the Embedding Weights? We tried Fisher, VWR and EMS but we were told that they don't have them. Thanks, Nena Nena Dimaano Hoffmann La Roche 340 Kingsland Ave. Nutley, NJ 07110 tel: 973-235-3953 fax: 973-235-4710 email: Nena.Dimaano@roche.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Jan 2 15:22:11 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 2 15:19:58 2007 Subject: AW: [Histonet] dehydration after staining In-Reply-To: <661614.77046.qm@web61222.mail.yahoo.com> Message-ID: <001901c72eb4$11207800$c812a8c0@dielangs.at> Ren?, I thought about solving the last of the bound water out of protein, if this is even possible. I?ve read, that overdehydration while embedding changes the refraction of collagen (Histologic). And that would enhance the glassy effect. Butylacetat is like aceton, the slides dry very fast, and the (own) skin is rendered white after contact. The positives about that are, that the slides are beautiful clean and clear and that they smell only for a few minutes. Gudrun From contact <@t> excaliburpathology.com Tue Jan 2 15:35:54 2007 From: contact <@t> excaliburpathology.com (P Pierce) Date: Tue Jan 2 15:36:02 2007 Subject: [Histonet] Slides Message-ID: <20070102213554.41194.qmail@web50103.mail.yahoo.com> I know it is winter, but if you have access to a pond, pond water is teaming with lots of little critters swimming around. The dirtier the better. He can place a drop of the pond water on the slide and watch them swim. That is what I did eons ago when I got my microscope for Christmas. Fish tank water would also work. Methylene blue would stain them too and can be diluted very weak and will still work. Find insects and leaves and just place parts of them them in water under a coverslip. That way the coverslip can be reused. Crush salt and sugar crystals into smaller bits and look at them dry. Have him experiment with different stains around the house like food coloring, iodine, etc. Watch different liquids dry and see what kind of crystals they form. We tend to just think of tissue since that is what we work with. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From ahacking <@t> yahoo.com Tue Jan 2 15:37:48 2007 From: ahacking <@t> yahoo.com (Dr. Adam Hacking, PhD) Date: Tue Jan 2 15:37:57 2007 Subject: [Histonet] maximize the hardness of MMA In-Reply-To: <6.2.5.6.2.20070102135011.01c48f30@vet.upenn.edu> Message-ID: <867324.98515.qm@web30813.mail.mud.yahoo.com> Dear all, Thank you for your responses. Pam as requested here is some background information. Please don't fall asleep :-) I have a technique where I coat a nylon rod with a homogeneous sub micron thick layer of titanium. These rods are implanted in the femurs of rats or mice and the tissue response to the implant is assessed. The goal of the model is to assess tissue and cell response to titanium implants using traditional light microscopy techniques (usually we just asses bone via backscattered electron microscopy [BSEM]) The model works quite well, and as far as the tissue response is concerned the tissue responds as if it were a solid titanium implant. One ongoing problem is separation of the methylmethacrylate (and tissue) from interface with the nylon rod during sectioning. I am very familiar with embedding Ti implants and sectioning with a diamond saw however microtome sectioning is new to me (thankfully we do have a very capable technician who is familiar with MMA embedding and sectioning) but offers a number of tests that cannot be performed by BSEM. I tried a batch of MMA that was activated by addition of benzoyl peroxide [BPO] and this seemed harder than our tech's low temp method of embedding. The sections were much better and I attributed this to the additional hardness of the resin and its resistance to compression during sectioning. However some of our tests (ALP, TRAP) did not work. We have tried increasing the BPO concentration and this has some effect. Karen, I will recommend skipping the n-butyl phthalate. Here are our mixtures of MMA. All are fixed in para formaldehyde for 48 hours. Harder MMA tested (classic implant MMA) Dehydrate samples : 75, 85, 95 % EtOH 48 hrs each Defat 1:1 Ether : Acetone 48 hrs Final Dehydrate 100% EtOH 48 hrs Cold MMA (MMA with0.5% BPO) 48 hrs Hot MMA (MMA with0.5% BPO and heated at 56 C for 4-5 hours) Embed under alternating vacuum 48 hrs Low TEMP MMA (RG Erben The Journal of Histochemistry & Cytochemistry 1997) 75, 85, 95 % EtOH 48 hrs each 100% EtOH 24 hrs 100% Zylene 48 hrs 48 hrs - MMA Solution I consisted of 60 ml MMA (Merck; containing 100 ppmhydrochinon) 35ml butylmethacrylate (Sigma; containing 10 ppm hydrochinon) 5 ml methylbenzoate 1.2 ml polyethylene glycol 400. @ 4C 48 hrs - 100 ml MMA solution above plus with 0.4 g dry benzoyl peroxide@ 4C Embedded - 100 ml MMA solution above plus with 0.8 g dry benzoyl peroxide @ 4C Thank you again. Adam Hacking Pamela Marcum wrote: Hi Adam, The best way to have the hardest MMA is to use straight MMA, with no dibutyl phthalate. It is a mixture of MMA as the plastic and a small amount of benzyol peroxide. IF you can tell me (us) what you wish to embed and how you will section and stain it (as well as view light microscopy or EM) the information would make it easier to help you with the problem. Pam Marcum At 01:14 PM 1/2/2007, you wrote: >Hi, > >Does anyone know how to increase or maximize the hardness of MMA? > >Are there harder embedding materials ? > >Many thanks > >Adam Hacking. PhD > > >________________________________________________________________ >Dr. S Adam Hacking, Post Doctoral Fellow >JTN Wong Laboratories for Mineralized Tissue Research, >Center for Bone and Periodontal Research, >McGill University 740 Dr. Penfield Ave. Rm. 2201 >Montreal, QC, Canada H3A 1A4 >Ph.: 514-398-5112 >Fax: 514-398-4020 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ________________________________________________________________ Dr. S Adam Hacking, Post Doctoral Fellow JTN Wong Laboratories for Mineralized Tissue Research, Center for Bone and Periodontal Research, McGill University 740 Dr. Penfield Ave. Rm. 2201 Montreal, QC, Canada H3A 1A4 Ph.: 514-398-5112 Fax: 514-398-4020 From vazquezr <@t> ohsu.edu Tue Jan 2 16:13:19 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Jan 2 16:13:47 2007 Subject: [Histonet] Slides Message-ID: Amy, Go to a Toys R Us and they have a good selection of different kinds of slides and they are not that expensive. Robyn OHSU >>> "Senn, Amy" 1/2/2007 1:02 PM >>> My 7 year old son got a microscope for Christmas. He's very excited to look at slides! The microscope came with 3 slides: Pine needle, onion peel, and pumpkin stem. Problem is, he's bored with those slides and wants more. I made him a few here in the surgical path dept. on the cryostat, but I'm not directly in histology anymore, so I can't just cut a few extras and stain them myself. I don't know where to get more slides for him, short of paying 29.99 PLUS shipping and handling for only TEN slides from the place where I got the microscope to begin with. Anyone have some extras they'd like to share? He'd really like to see some colors and 'funky designs' as he likes to say. We made a few from his spit and even one from his brother's boogers (lol) but he's getting tired of the same old stuff & is acutally really interested in what the 'funky colors' mean.... I'd be willing to pay postage..... Thanks so much for any help!! Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtibudan <@t> hotmail.com Tue Jan 2 16:47:13 2007 From: mtibudan <@t> hotmail.com (martin tibudan) Date: Tue Jan 2 16:47:23 2007 Subject: [Histonet] Cryostat Purchasing Message-ID: We are in an ophthalmology department, and we are purchasing either a Leica CM 1800 or a Vibratome Ultrapro 5000 cryostat. The main uses of the cryostat will be for tissues related to the eye. The minotome we currently use is beginning to show inconsistencies. We would appreciate any input in either of the cryostats. Martin _________________________________________________________________ >From photos to predictions, The MSN Entertainment Guide to Golden Globes has it all. http://tv.msn.com/tv/globes2007/ From mtibudan <@t> hotmail.com Tue Jan 2 17:14:38 2007 From: mtibudan <@t> hotmail.com (martin tibudan) Date: Tue Jan 2 17:14:51 2007 Subject: [Histonet] Vibratome vs Bright Message-ID: In my search for the "Vibratome Ultrapro 5000", I came across a "Bright Ultrapro 5000". Are these machines manufactured by the same company? They look identical. Can anyone clarify this for me? Martin _________________________________________________________________ Type your favorite song.  Get a customized station.  Try MSN Radio powered by Pandora. http://radio.msn.com/?icid=T002MSN03A07001 From Lchausse <@t> nmh.org Tue Jan 2 18:40:56 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Tue Jan 2 18:41:19 2007 Subject: [Histonet] Renal bxs for IF Message-ID: Hello - Just wondering how various labs out there are addressing the CAP reg below. Thanks for any input in advance. ANP.21850 Are appropriate positive and negative controls performed with each case tested using immunofluorescence? NOTE: Internal antigens serve as positive controls (e.g., IgA in tubular cases, IgG in protein droplets, and C3 in blood vessels). Non-reactive elements in the patient specimen may serve as a negative tissue control. A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen. Reference: Walker PD, et al. Practice guidelines for the renal biopsy. Mod. Pathol. 2004;17:1555-1563. ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From mrsseagle <@t> yahoo.com Tue Jan 2 20:14:21 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Tue Jan 2 20:14:29 2007 Subject: [Histonet] Certificate Maintenance Program?? Message-ID: <20070103021421.66159.qmail@web51811.mail.yahoo.com> Hello Everyone, I am a newly certified HT and was interested in learning a little more about the certificate maintenace that is required every three years to keep your certification valid. I have read all the information on tha ASCP web site but was interested in the programs or ways that some of you may use to earn your 36pts required along with any other information you may can give me that is not covered on the web site. thanks for your help, Michelle Seagle Rutherford Hospital Rutherfordton,NC __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Karen.Tamul <@t> invitrogen.com Tue Jan 2 20:37:38 2007 From: Karen.Tamul <@t> invitrogen.com (Tamul, Karen) Date: Tue Jan 2 20:38:26 2007 Subject: [Histonet] RE: Histonet Digest, Vol 38, Issue 2 Message-ID: Please unsubscribe me. Karen Tamul Karen.tamul@invitrogen.com From anthony.joshua <@t> utoronto.ca Tue Jan 2 21:46:06 2007 From: anthony.joshua <@t> utoronto.ca (Anthony joshua) Date: Tue Jan 2 21:46:19 2007 Subject: [Histonet] Immunofluorescent foci moving? Message-ID: <7CF68C0A-3FAA-4DE5-82D7-13E8946035E1@utoronto.ca> Hoping for some help here, Im doing FITC staining of some markers of DNA damage foci (gH2Ax, MDC1) in both paraffin and cell lines and find that apart from the nuclear staining pattern that is expected I see that the foci are moving in a kind of brownian motion. Im sure its not bacteria, as they would be dead long ago. Im using a primary and a labeled secondary. Any opinion as to what causes this or what I can do about it? More air-drying? better fixative? I note there was a previous post about this about a year ago but no one replied. Thanks Anthony BSc(Med) MBBS FRACP Fellow in Cellular and Molecular Biology Department of Medical Oncology Princess Margaret Hospital 610 University Avenue M5G2M9 Toronto "Don't worry about the world coming to an end today. It's already tomorrow in Australia." (Charles Schultz) From JWEEMS <@t> sjha.org Wed Jan 3 07:06:42 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jan 3 07:07:02 2007 Subject: [Histonet] Immuno Disclaimer Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2E37@sjhaexc02.sjha.org> When you guys put the disclaimer in your reports regarding the ASR antibodies, do you list those antibodies, or just use the general statement suggested in the CAP checklist? Thanks and Happy New Year to all!!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From tbraud <@t> holyredeemer.com Wed Jan 3 08:27:52 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jan 3 08:29:44 2007 Subject: [Histonet] Immuno Disclaimer In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2E37@sjhaexc02.sjha.org> Message-ID: General, worded closely to CAP, but lists the exception of Her-2-Neu and EGFR which are the FDA approved tests. Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, January 03, 2007 8:07 AM To: Histonet Subject: [Histonet] Immuno Disclaimer When you guys put the disclaimer in your reports regarding the ASR antibodies, do you list those antibodies, or just use the general statement suggested in the CAP checklist? Thanks and Happy New Year to all!!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From akemiat3377 <@t> yahoo.com Wed Jan 3 08:59:30 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 3 08:59:38 2007 Subject: [Histonet] bright cryostat Message-ID: <630058.47751.qm@web31308.mail.mud.yahoo.com> Hi If you call Hacker instruments at (803) 712-6100, they can clarify the difference for you. I believe they carry the bright cryostat. Sincerely, Akemi Allison-Tacha, BS HT (ASCP) HTL President Phoenix Lab Consulting & Staffing E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From pmcardle <@t> ebsciences.com Wed Jan 3 09:04:10 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed Jan 3 09:04:22 2007 Subject: [Histonet] maximize the hardness of MMA In-Reply-To: <20070102181426.18628.qmail@web30803.mail.mud.yahoo.com> References: <20070102181426.18628.qmail@web30803.mail.mud.yahoo.com> Message-ID: <459BC5EA.6080403@ebsciences.com> Hello: If the MMA you refer to is a Technovit product: we are the US distributor of Heraeus Kulzer's Technovit kits including 7100, 8100 and 9100. In the case of their MMA kits which consist of (5) components, component 5 is the softener, so a smaller proportion results in a harder block. It is possible to eliminate component 5 altogether, but Kulzer "officially" recommends against this since other undesirable side effects (brittleness, etc.) can occur. However, you may want to try this with some non-critical samples (or no sample at all) to see how the blocks cut. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway; please do the right thing and make it go away. Thank you. Dr. Adam Hacking, PhD wrote: > Hi, > > Does anyone know how to increase or maximize the hardness of MMA? > > Are there harder embedding materials ? > > Many thanks > > Adam Hacking. PhD > > > ________________________________________________________________ Dr. S Adam Hacking, Post Doctoral Fellow > JTN Wong Laboratories for Mineralized Tissue Research, > Center for Bone and Periodontal Research, > McGill University 740 Dr. Penfield Ave. Rm. 2201 > Montreal, QC, Canada H3A 1A4 > Ph.: 514-398-5112 > Fax: 514-398-4020 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From froyer <@t> bitstream.net Wed Jan 3 09:05:23 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Jan 3 09:05:40 2007 Subject: [Histonet] Slides In-Reply-To: Message-ID: <002f01c72f48$9821a100$7701a80a@Ford> Amy, I carry a line of educational prepared slides that are very economical and contain elements that you might not be able to find at a toy store. You can check them out by following the link below... http://www.cnascientific.com/Product_View.asp?ID=230 These box sets range in price from $14.00 to $25.00 and include between 12 slides (Elementary Level) and 25 slides (Intermediate Level & High School Level). There are smaller sets available also, but are not shown on the web site. Each set contains 5 slides and range in price from $5.95 and $8.75. "Blood and Guts" ... brain, heart, blood cells, etc. "Creepy Crawlies" ... (do you dare to find out?) "Extraordinary Ordinary" ... hair, cork, fibers, etc. "Incredible Edible Plants" ... roots, stems, & leaves of the food we eat "Wicked Wings" ... Insect wings: bees, butterfly, mosquito, etc. PLUS; in addition there are over 300 additional slides that can be ordered individually. Contact me off list for more information. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Tuesday, January 02, 2007 4:13 PM To: histonet@lists.utsouthwestern.edu; ASenn@mercy.pmhs.org Subject: Re: [Histonet] Slides Amy, Go to a Toys R Us and they have a good selection of different kinds of slides and they are not that expensive. Robyn OHSU >>> "Senn, Amy" 1/2/2007 1:02 PM >>> My 7 year old son got a microscope for Christmas. He's very excited to look at slides! The microscope came with 3 slides: Pine needle, onion peel, and pumpkin stem. Problem is, he's bored with those slides and wants more. I made him a few here in the surgical path dept. on the cryostat, but I'm not directly in histology anymore, so I can't just cut a few extras and stain them myself. I don't know where to get more slides for him, short of paying 29.99 PLUS shipping and handling for only TEN slides from the place where I got the microscope to begin with. Anyone have some extras they'd like to share? He'd really like to see some colors and 'funky designs' as he likes to say. We made a few from his spit and even one from his brother's boogers (lol) but he's getting tired of the same old stuff & is acutally really interested in what the 'funky colors' mean.... I'd be willing to pay postage..... Thanks so much for any help!! Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 3 09:18:10 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 3 09:18:20 2007 Subject: [Histonet] Mitochondria Stain Message-ID: <66455.70917.qm@web31314.mail.mud.yahoo.com> Hello, The Biological Stain Commision's website has a procedure for Altman's Stain for Mitochodria. It provides solution preparations as well as the procedure. It is listed under protocol featured stain, just under acid fuchsin. The web address is www.biostains.org Good Luck & Happy New Year Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From RJLevier <@t> LancasterGeneral.org Wed Jan 3 09:58:18 2007 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Wed Jan 3 09:58:30 2007 Subject: [Histonet] Clear Rite 3 Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2D6E207@MAIL-LR.lha.org> Is there anyone that is using Clear Rite 3 and if so how do you dispose of it and do you have any literature saying that it is ok to do what you are doing? Please help and Thanks in Advance. Rebecca J. LeVier HT(ASCP) Histology Supervisor Phone: (717)-544-5065 Fax: (717)-544-5457 Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From stamptrain <@t> yahoo.com Wed Jan 3 10:24:43 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed Jan 3 10:24:52 2007 Subject: [Histonet] Clear Rite 3 In-Reply-To: <0FDBF29200C637468CCC1F9E7E85A3D2D6E207@MAIL-LR.lha.org> Message-ID: <124356.99479.qm@web50302.mail.yahoo.com> We dispose of Clear Rite 3 as "Xylene". That is what our Safety people instructed us to do. Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT --- "LeVier, Rebecca J" wrote: > Is there anyone that is using Clear Rite 3 and if so > how do you dispose > of it and do you have any literature saying that it > is ok to do what you > are doing? > > Please help and Thanks in Advance. > > Rebecca J. LeVier HT(ASCP) > Histology Supervisor > Phone: (717)-544-5065 > Fax: (717)-544-5457 > Email: rjlevier@lancastergeneral.org > > > > Confidentiality Notice: This e-mail message, > including any > attachments, is for the sole use of intended > recipient(s) and may > contain confidential and privileged information. > Any unauthorized > review, use, disclosure or distribution is > prohibited. If you are not > the intended recipient, please contact the sender by > reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bruno.ping <@t> nhs.net Wed Jan 3 10:30:44 2007 From: bruno.ping <@t> nhs.net (Ping Bruno (Royal Surrey County Hospital NHS Trust)) Date: Wed Jan 3 10:30:56 2007 Subject: [Histonet] CISH and Her-2 and beyond Message-ID: <20070103163044.ACB25759@ms08.swi.contact.secure-ops.net> Hi everyone, My name is Bruno and I am a Biomedical Scientist based in the UK.We are involved in Her-2 Cish (the only center in the UK providing a diagnostic service based on CISH for HEr-2) and we would like to learn more about this essay that we started almost a year ago.We have experience using CISH kits from 3 different companies and also other reagents involved in the process. We also are interested in other procedures that would involve CISH (not only HEr-2) If you have any tips or useful information you would like to share dont hesitate to contact us!Sample processing tips would be appreciated also. Regards Bruno Ping ********************************************************************** Information in this message may contain confidential and privileged information. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. NHSmail is used daily by over 100,000 staff in the NHS. Over a million messages are sent every day by the system. To find out why more and more NHS personnel are switching to this NHS Connecting for Health system please visit www.connectingforhealth.nhs.uk/nhsmail ********************************************************************** From akemiat3377 <@t> yahoo.com Wed Jan 3 11:11:35 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 3 11:11:44 2007 Subject: [Histonet] CAT Hematoxylin Message-ID: <965398.81621.qm@web31315.mail.mud.yahoo.com> Hello & Happy New Year! Regarding a histonet e-mail question about who developed CAT Hematoxylin. Here is the clarification. There were actually three histologists involved with the development. While I was working at Biocare, Chuck Churukian gave me the modification for Mayer's Hematoxylin, I came up with the proprietary ingredient for stabalization & David Tacha from Biocare suggested the pH portion. Thus the CAT (Churukian-Allison-Tacha) I started my research in 2001 & officially released it in 2002 at the NSH meeting. David & I developed it to be used for IHC, H&E's & SS. I was the person who actually made-up the hematoxylin from A-Z. & learned alot about bulk manufacturing & pH. Happy New Year, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From akemiat3377 <@t> yahoo.com Wed Jan 3 12:09:11 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 3 12:09:19 2007 Subject: [Histonet] STF suppliers Message-ID: <662451.9259.qm@web31304.mail.mud.yahoo.com> Hello & Happy New Year, Listed are Commerical Fixative Suppliers, Composition and Main Action: Perhaps you can go on the web & look-up # Let me know if this helps. 1) Tissue-Tek (STF) from Miles (Streck Laboratories) 2-Bromo-2nitropropan-1, 3-diol (bronopol), ] 3%; diazolindinyl urea, ]5%; zinc sulfate, ]5%; formaldehyde,] 0.1%) Main Action: Non-cross-linking, noncoagulative 2) Histochoice from Amresco Aldehyde addition compounds, buffers, salts, stabilizers Main Action: Non-cross linking 3) Krofix from Merck, Darmstadt Alcohol polyethylene glycols Main Action: Protein coagulant 4) No Tox from Earth Safe Industries Aq. ethanol, bis carbonyl componds, bacteriocides, virocides, fungicides humectants Main Action: Protein cross-linking 5) Omnifix II from An-Con Genetics Ethanol 36%, ethylene glycol, acetic acid, sodium chloride zinc chloride Main Action: Watere removal, nucleic acid precipitation, stabalization of protein conformation Happy New Year Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 function rmvScroll( msg ) { var delta = msg.offsetHeight - msg.clientHeight; delta = ( isNaN( delta )? 1 : delta + 1 ); if ( msg.scrollHeight > msg.clientHeight ) { msg.style.height = ( msg.scrollHeight + delta ) + "px"; } delta = msg.offsetWidth - msg.clientWidth; delta = ( isNaN( delta )? 1 : delta + 1 ); if ( msg.scrollWidth > msg.clientWidth ) { msg.style.width = ( msg.scrollWidth + delta ) + "px"; } msg.style.overflow = "hidden"; msg.style.visibility = "visible"; } var msg = document.getElementById( "message" ); if ( msg & "undefined" != typeof msg ) { rmvScroll( msg ); } From Bauer.Karen <@t> mayo.edu Wed Jan 3 12:11:22 2007 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Wed Jan 3 12:11:53 2007 Subject: [Histonet] Stainless Steel 30 capacity slide racks Message-ID: Hi to all. Our department has 3 stainless steel, 30 slide capacity staining racks that we are willing to give away. We never use them and are cleaning house. They are going on a first come, first serve basis. Anyone want them? Karen L. Bauer, HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Bauer.Karen <@t> mayo.edu Wed Jan 3 12:27:59 2007 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Wed Jan 3 12:28:34 2007 Subject: [Histonet] Slide racks gone. Message-ID: Just wanted all to know that the slide racks have been spoken for. Happy New Year to all! Karen ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Jackie.O'Connor <@t> abbott.com Wed Jan 3 12:42:03 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jan 3 12:42:38 2007 Subject: [Histonet] Formalin Substitutes - an invitation to vendors In-Reply-To: Message-ID: I'm inviting any vendors of formalin substitutes to hawk their wares to me at my email address. Since STF is no more, I'm looking for an equivalent replacement. Now's your chance. Jackie O' From gcallis <@t> montana.edu Wed Jan 3 12:49:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jan 3 12:49:36 2007 Subject: [Histonet] Thoughts about STF fixative In-Reply-To: <662451.9259.qm@web31304.mail.mud.yahoo.com> References: <662451.9259.qm@web31304.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20070103114658.01b51dc0@gemini.msu.montana.edu> On looking at the chemical content of STF, it appears to me that it is NOT totally formalin free with the 0.1% formaldehyde content. At 11:09 AM 1/3/2007, you wrote: >Hello & Happy New Year, > > Listed are Commerical Fixative Suppliers, Composition and Main > Action: Perhaps you can go on the web & look-up # Let me know if this helps. > > 1) Tissue-Tek (STF) from Miles (Streck > Laboratories) 2-Bromo-2nitropropan-1, 3-diol (bronopol), ] 3%; > diazolindinyl urea, ]5%; zinc sulfate, ]5%; formaldehyde,] 0.1%) Main > Action: Non-cross-linking, noncoagulative > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Robinsoc <@t> mercyhealth.com Wed Jan 3 12:53:29 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Wed Jan 3 12:53:48 2007 Subject: [Histonet] Fungal controls Message-ID: All, Does anyone have any wet tissue with fungus that they would be willing to share? Thanks. Cindi Robinson, HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 801 5th St Sioux City IA 51101 From RJLevier <@t> LancasterGeneral.org Wed Jan 3 13:05:43 2007 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Wed Jan 3 13:05:59 2007 Subject: [Histonet] (no subject) Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2D6E211@MAIL-LR.lha.org> I would like to be removed from the histonet list. I thought that this site was for serious help not for people to do whatever they like. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From DennisH <@t> cookchildrens.org Wed Jan 3 13:59:16 2007 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Wed Jan 3 14:00:44 2007 Subject: [Histonet] Tissue Processors Message-ID: Hello HistoNet, I am in the process of evaluating new tissue processors. We are a small histo lab and average 50 blocks per day (Sometimes 20, extremely busy days 130). We process a lot of GI biopsies, tonsils, etc as most children's hospital's do. We need a good work horse that can handle the occasional breast tissue, tumor, or colon, but will give us excellent results with the smallest of biopsies without the troubles of breaking down every month. Has anyone evaluated the most common tissue processors lately and what were your findings? No vendors, please. We are not considering microwave processing due to our small size and the costs at this time. Thank You all, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 3 14:00:58 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Jan 3 14:01:22 2007 Subject: [Histonet] (no subject) Message-ID: Rebecca Am I missing something here as I am not sure what you are referring to? If you have some constructive criticism of the Histonet I hope that you will share it. It has been my experience that the people using Histonet have always been willing to discuss any problems. If you would prefer you can always sent to me directly. Thank you Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: Wednesday, January 03, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I would like to be removed from the histonet list. I thought that this site was for serious help not for people to do whatever they like. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 3 14:06:13 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 3 14:06:23 2007 Subject: [Histonet] article on STF Message-ID: <792588.43301.qm@web31305.mail.mud.yahoo.com> The information I abstracted was fairly indepth discussing Materials & Methods, Tissue, Procedure, Results (Time Dependent Depth of Fixation) Mode of Fixation, Electron Microscopy, Light Microscopy, IHC The information came from Biotechnic & Histochemistry 1052-0295/97273-282 by Williams & Wilkins Volume 72. Number 5 Commercial Formalin Suubstitutes for Histopathology by Poul Prento & Hans Lyon. Department of Pathology, Hvidovre Hospital, University of Copenhagen. Hope this helps. Happy New Year, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From Jessica <@t> medstaffservices.com Wed Jan 3 14:13:55 2007 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Jan 3 14:14:08 2007 Subject: [Histonet] EMPLOYMENT OPPROTUNITIES Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: ~$30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k 3. Generalist Hours: Monday - Friday, Day Shift Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From JWEEMS <@t> sjha.org Wed Jan 3 14:45:10 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jan 3 14:45:29 2007 Subject: [Histonet] (no subject) Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2E84@sjhaexc02.sjha.org> Ditto. I'm lost. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, January 03, 2007 3:01 PM To: LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Rebecca Am I missing something here as I am not sure what you are referring to? If you have some constructive criticism of the Histonet I hope that you will share it. It has been my experience that the people using Histonet have always been willing to discuss any problems. If you would prefer you can always sent to me directly. Thank you Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: Wednesday, January 03, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I would like to be removed from the histonet list. I thought that this site was for serious help not for people to do whatever they like. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Amanda.Garcia <@t> TriadHospitals.com Wed Jan 3 15:11:43 2007 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Wed Jan 3 15:13:26 2007 Subject: [Histonet] unsubscribe Message-ID: <8B63039C9DF4554C8FDBF31235F0E1480285B8FE@CPRTEVS02.triadhospitals.net> Please unsubscribe me. > Amanda (Amy) Garcia > > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From bperunovic <@t> doctors.org.uk Wed Jan 3 16:08:39 2007 From: bperunovic <@t> doctors.org.uk (Branko Perunovic) Date: Wed Jan 3 16:08:42 2007 Subject: [Histonet] unsubscribe Message-ID: <000c01c72f83$b97ff320$1701a8c0@banenotebook> unsubscribe From bperunovic <@t> doctors.org.uk Wed Jan 3 16:09:19 2007 From: bperunovic <@t> doctors.org.uk (Branko Perunovic) Date: Wed Jan 3 16:09:21 2007 Subject: [Histonet] unsubscribe Message-ID: <001101c72f83$d1526e10$1701a8c0@banenotebook> Unsubscribe Me please From anh2006 <@t> med.cornell.edu Wed Jan 3 17:51:35 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Jan 3 17:51:56 2007 Subject: [Histonet] ISH protocol with DIG-labeled probes Message-ID: Dear All, I was wondering if someone has a tried and true ISH protocol (I know, a stretch!) which they could send to me for paraffin sections using DIG labeled probes? I have tried some homemade mix-and-match protocols and am at my wits end getting the specificity to be accurate. I know my probes are good as I use them for other purposes with good success so that isn't it. Help! Thank you in advance, Andrea -- From liz <@t> premierlab.com Wed Jan 3 18:14:38 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 3 18:05:33 2007 Subject: [Histonet] ISH protocol with DIG-labeled probes In-Reply-To: Message-ID: <000401c72f95$527f5e60$0d00a8c0@domain.Premier> Andrea Try Genedetect website http://www.genedetect.com/ it has a lot of good info on ISH protocols. We're working on some biotinylated probes from them right now, but we have only tried one run and we got no staining so far. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: Wednesday, January 03, 2007 4:52 PM To: Histonet Subject: [Histonet] ISH protocol with DIG-labeled probes Dear All, I was wondering if someone has a tried and true ISH protocol (I know, a stretch!) which they could send to me for paraffin sections using DIG labeled probes? I have tried some homemade mix-and-match protocols and am at my wits end getting the specificity to be accurate. I know my probes are good as I use them for other purposes with good success so that isn't it. Help! Thank you in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1955 (20070103) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jmaass <@t> frii.com Wed Jan 3 22:47:03 2007 From: jmaass <@t> frii.com (Janet Maass) Date: Wed Jan 3 22:47:21 2007 Subject: [Histonet] register Message-ID: <000801c72fbb$6187fb80$0200a8c0@oemcomputer> From sonya.martin <@t> soton.ac.uk Thu Jan 4 04:02:36 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Thu Jan 4 04:03:05 2007 Subject: [Histonet] RE:Immunofluorescent foci moving? Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34CD@ISS-CL-EX-V1.soton.ac.uk> I used to have this problem when staining transcription sites in the nuclues of cultured cells - we thought it was just clumps of antibody getting trapped. I have found that FITC labeled antibody preps often need to be centrifuged as some of the FITC seems to come out into the solution. We centrifuged our antibody dilutions before use and increased the number and length of washes and added 0.1% triton to the washes. This seemed to help but occaisionally I still saw some moving foci so if you get any interestng replies please let me know. Best of luck Sonya From sonya.martin <@t> soton.ac.uk Thu Jan 4 04:08:29 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Thu Jan 4 04:08:45 2007 Subject: [Histonet] Freezing tissues Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34CE@ISS-CL-EX-V1.soton.ac.uk> Hi there, I need to freeze some mouse spleens tomorrow for cryosectioning. Usually I cut the spleen in half and place it in OCT in a foil mould. I then place the foil mold in a petri dish of isopentane sitting on dry ice. This seems to work well and I dont have problems with ice crystals or freezing artifacts. However, after the holidays there is not a drop of dry ice to be found in our institute so I need some advice on another method - possibly using liquid nitrogen. I've read a number of methods whereby you immerse the tissue directly in the LN, hold the tissue in the LN vapours or do as I do with isopentane but have the petri dish floating on LN (is this possible?). Any suggestions greatly appreciated! Thanks Sonya From lnlj <@t> novonordisk.com Thu Jan 4 04:31:02 2007 From: lnlj <@t> novonordisk.com (LNLJ (Lene Lyngsie Jensen)) Date: Thu Jan 4 04:31:12 2007 Subject: [Histonet] Perfusion fixation of pig lungs Message-ID: <48BB4C2CF37C1843930ECAC01475F835F6F393@EXDKBA025.corp.novocorp.net> Hey. I hope that someone here can help me. We are planning to do some research in pig lungs. Due to this investigation it is very important to have a fast and effective fixation. We will therefore try to make a perfusion fixation. But a pig is a very large animal and therefore we would like to limit the fixation only to the lung region, and not a full body perfusion fixation. Is there anyone how has experience with perfusion fixation limit to only one organ in pigs? All suggestion will be read with enthusiasm. Experience from similar fixation methods in other animals is welcome as well. Thanks. ________________________________________________ Lene Lyngsie Jensen From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jan 4 04:37:25 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jan 4 04:37:34 2007 Subject: [Histonet] Perfusion fixation of pig lungs Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012470A5@wahtntex2.waht.swest.nhs.uk> Cut out the lungs, attach the trachea to a header tank sufficiently high above the lungs to give one atmosphere of pressure and then pump the formalin that leaks out back up to the header tank. You need sticky backed tape and cardboard (English joke). Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com It's not what we eat but what we digest that makes us strong; not what we gain but what we save that makes us rich; not what we read but what we remember that makes us learned; and not what we profess but what we practice that gives us integrity. --Francis Bacon This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From PMonfils <@t> Lifespan.org Thu Jan 4 09:47:29 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jan 4 09:47:39 2007 Subject: [Histonet] Freezing tissues In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E34CE@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BE8@LSRIEXCH1.lsmaster.lifespan.org> Isopentane will freeze before it reaches the temperature of liquid nitrogen. I often freeze tissues with liquid nitrogen, in fact it is my preferred method. Pour the nitrogen into a suitable container. I use a shallow styrofoam box, the lower portion of an ordinary styrofoam shipping container that I cut down to size. But just about any container will do, plastic or pyrex glass. Wait a minute until the boiling action stops. Put your specimen and OCT in the mold as usual, but make the mold so that it extends well above the surface of the OCT. I usually use polyethylene molds but I also use foil sometimes, for specimens that don't fit well into the polyethylene molds. Float the mold on top of the nitrogen. If it won't float without tipping over, hold it in position with forceps. When it is almost frozen, having just a small window of unfrozen OCT on the top about 1/3 the width of the block, remove it and transfer directly into the cryostat or the -80 freezer. The rest of the OCT will quickly freeze from the frozen OCT underneath. Don't let the block sink in the nitrogen, and don't leave it longer than I described above or the OCT may crack. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Martin S. > Sent: Thursday, January 4, 2007 2:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Freezing tissues > > Hi there, > > I need to freeze some mouse spleens tomorrow for cryosectioning. Usually > I cut the spleen in half and place it in OCT in a foil mould. I then > place the foil mold in a petri dish of isopentane sitting on dry ice. > This seems to work well and I dont have problems with ice crystals or > freezing artifacts. However, after the holidays there is not a drop of > dry ice to be found in our institute so I need some advice on another > method - possibly using liquid nitrogen. I've read a number of methods > whereby you immerse the tissue directly in the LN, hold the tissue in > the LN vapours or do as I do with isopentane but have the petri dish > floating on LN (is this possible?). > Any suggestions greatly appreciated! > > Thanks > Sonya > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mcauliff <@t> umdnj.edu Thu Jan 4 10:17:52 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jan 4 10:18:42 2007 Subject: [Histonet] Perfusion fixation of pig lungs In-Reply-To: <48BB4C2CF37C1843930ECAC01475F835F6F393@EXDKBA025.corp.novocorp.net> References: <48BB4C2CF37C1843930ECAC01475F835F6F393@EXDKBA025.corp.novocorp.net> Message-ID: <459D28B0.7060808@umdnj.edu> Hi Lene: 1. Decide if you want to perfuse via the vascular system (washing out the blood) or instill fixative via the trachea and bronchial tree. Note that the position of the animal during instillation will determine which regions of the lung are fixed first (or at all!). Study of the anatomy of the bronchial tree of the pig will pay dividends. 2. Decide if you are going to look at the entire lung or just one (or more) small regions. I suggest looking at one bronchopulmonary segment from one (or more?) lobes to conserve resources and minimize variation from animal to animal. If that is your decision then you can do a little dissection and either perfuse that segment or instill into that segment. Good luck! Geoff LNLJ (Lene Lyngsie Jensen) wrote: >Hey. > >I hope that someone here can help me. > >We are planning to do some research in pig lungs. Due to this >investigation it is very important to have a fast and effective >fixation. > >We will therefore try to make a perfusion fixation. But a pig is a very >large animal and therefore we would like to limit the fixation only to >the lung region, and not a full body perfusion fixation. > >Is there anyone how has experience with perfusion fixation limit to only >one organ in pigs? > >All suggestion will be read with enthusiasm. > >Experience from similar fixation methods in other animals is welcome as >well. > >Thanks. >________________________________________________ > >Lene Lyngsie Jensen >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Thu Jan 4 10:27:19 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jan 4 10:27:25 2007 Subject: Liquid nitrogen, mouse spleen Re: [Histonet] Freezing tissues In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E34CE@ISS-CL-EX-V1.soto n.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4023E34CE@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <6.0.0.22.1.20070104092247.01b4d3e8@gemini.msu.montana.edu> Yes, your very last suggestion will work perfectly. We place a plastic petri dish IN the liquid nitrogen, then you can set the OCT embedded spleen in the petri dish. We have shattered spleen and if we do isopentane cooled by liquid nitrogen, or direct immersion into liquid nitrogen. Just make sure the petri dish is supported so it does not tip over in the Liq N2, and do not allow Liq N2 into the dish. You need to replenish the liq nitrogen as it is important to maintain the dish directly in the N2. In other words, the dish goes canoeing in the Liq N2. Good luck At 03:08 AM 1/4/2007, you wrote: >Hi there, > >I need to freeze some mouse spleens tomorrow for cryosectioning. Usually >I cut the spleen in half and place it in OCT in a foil mould. I then >place the foil mold in a petri dish of isopentane sitting on dry ice. >This seems to work well and I dont have problems with ice crystals or >freezing artifacts. However, after the holidays there is not a drop of >dry ice to be found in our institute so I need some advice on another >method - possibly using liquid nitrogen. I've read a number of methods >whereby you immerse the tissue directly in the LN, hold the tissue in >the LN vapours or do as I do with isopentane but have the petri dish >floating on LN (is this possible?). >Any suggestions greatly appreciated! > >Thanks >Sonya > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Jan 4 10:35:50 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jan 4 10:35:55 2007 Subject: [Histonet] Re: Immunofluorescent foci moving? In-Reply-To: References: Message-ID: <6.0.0.22.1.20070104093449.01b53a08@gemini.msu.montana.edu> Vectashield also comes as a hard set. At 06:44 AM 1/4/2007, you wrote: >Dear all >Thanks for your help. I think the problem is that the mounting media is >vectashield which remains aqeous and does not hard set, I will change to >hard set media, as well as cetrifuging antibody dilutions and increasing >the washes >Thanks for your help >Anthony > >On 04/01/2007, at 5:36 AM, Edwards, R.E. wrote: > >>Could it be that the mounting media is incompletely dry?? >> >>-----Original Message----- >>From: >>histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony >>joshua >>Sent: 03 January 2007 03:46 >>To: >>histonet@lists.utsouthwestern.edu >>Subject: [Histonet] Immunofluorescent foci moving? >> >>Hoping for some help here, >>Im doing FITC staining of some markers of DNA damage foci (gH2Ax, >>MDC1) in both paraffin and cell lines and find that apart from the >>nuclear staining pattern that is expected I see that the foci are moving >>in a kind of brownian motion. Im sure its not bacteria, as they would be >>dead long ago. Im using a primary and a labeled secondary. Any opinion >>as to what causes this or what I can do about it? More air-drying? >>better fixative? I note there was a previous post about this about a >>year ago but no one replied. >>Thanks >>Anthony >> >>BSc(Med) MBBS FRACP >>Fellow in Cellular and Molecular Biology Department of Medical Oncology >>Princess Margaret Hospital 610 University Avenue >>M5G2M9 Toronto >>"Don't worry about the world coming to an end today. It's already >>tomorrow in Australia." (Charles Schultz) >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >BSc(Med) MBBS FRACP >Fellow in Cellular and Molecular Biology >Department of Medical Oncology >Princess Margaret Hospital >610 University Avenue >M5G2M9 Toronto >Pager -416-715-2594 >Lab- 416-946-4501 Ext 5012 (Rm 9-717) >Cell/ Mobile -416-671-1791 >"Don't worry about the world coming to an end today. It's already >tomorrow in Australia." (Charles Schultz) > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JEllin <@t> yumaregional.org Thu Jan 4 11:27:53 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Jan 4 11:28:14 2007 Subject: [Histonet] Wage and vacancy Message-ID: <29BE166A2CF48D459853F8EC57CD37E87A7B5B@EXCHANGECLUSTER.yumaregional.local> Thank you to everyone that help with their input on wage, vacany, and productivity. I still need more information but it is a great start in the right direction. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From vonavi <@t> inbox.lv Thu Jan 4 11:36:07 2007 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Thu Jan 4 11:36:24 2007 Subject: [Histonet] Technovit 9100 New Message-ID: <1167932167.459d3b070c428@www.inbox.lv> Hello, In our lab, we are working with EXAKT Cutting/Grinding system. For tissue embedding, we started to use Technovit 9100 New embedding medium. I have some questions regarding the use of this medium. My first question concerns the storage of destabilized basic solution. In the brochure, it is written that this solution can be stored at 4 degrees C for SHORTER PERIODS or at -20 degrees C for LONGER PERIODS of time. What does it mean to store for SHORTER and LONGER PERIODS of time? Days? Weeks? Years? My second question concerns re-use of some solutions. Is it possible to use the solutions for pre-infiltration and infiltration repeatedly? If it is possible, how should these solutions be stored? Thank you in advance! Best regards, Andrey Ivanov From anh2006 <@t> med.cornell.edu Thu Jan 4 12:01:04 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Jan 4 12:01:16 2007 Subject: [Histonet] Re: Liquid nitrogen, mouse spleen - Freezing tissues In-Reply-To: <6.0.0.22.1.20070104092247.01b4d3e8@gemini.msu.montana.edu> References: <71437982F5B13A4D9A5B2669BDB89EE4023E34CE@ISS-CL-EX-V1.soton.ac.uk> <6.0.0.22.1.20070104092247.01b4d3e8@gemini.msu.montana.edu> Message-ID: I second Gayle's technique described below. It is wonderful. After spending so many years fixated on isopentane in liquid nitrogen and getting cracked blocks in order to adhere with what was histologically correct, I have finally moved onto the floating boat technique and my frozens look nearly as good as paraffin sections. The only difference is that I use metal floating boats (read: sterilization trays) instead of plastic petri dishes. This way the blocks cool even quicker - better for the tissue and morphology - though they still don't shatter. I finally feel liberated :) At 9:27 AM -0700 1/4/07, Gayle Callis wrote: >Yes, your very last suggestion will work perfectly. We place a >plastic petri dish IN the liquid nitrogen, then you can set the OCT >embedded spleen in the petri dish. We have shattered spleen and if >we do isopentane cooled by liquid nitrogen, or direct immersion into >liquid nitrogen. Just make sure the petri dish is supported so it >does not tip over in the Liq N2, and do not allow Liq N2 into the >dish. You need to replenish the liq nitrogen as it is important to >maintain the dish directly in the N2. In other words, the dish goes >canoeing in the Liq N2. > >Good luck > -- From gcallis <@t> montana.edu Thu Jan 4 12:26:43 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jan 4 12:26:51 2007 Subject: [Histonet] Re: Liquid nitrogen, mouse spleen - Freezing tissues In-Reply-To: References: <71437982F5B13A4D9A5B2669BDB89EE4023E34CE@ISS-CL-EX-V1.soton.ac.uk> <6.0.0.22.1.20070104092247.01b4d3e8@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070104112114.01b54568@gemini.msu.montana.edu> Dear Andrea and all, Hooray for liberation! We will try the metal sterilization trays and what about disposable metal weighing dishes? I think these are out in vendor land too. Will be havet to go shopping. Another key issue with isopentane also know as 2 methyl butane is the storage problem. The "floating boat" method eliminates a rather nasty solvent, although you still need to use Liquid nitrogen with good ventilation. Gayle Callis At 11:01 AM 1/4/2007, you wrote: >I second Gayle's technique described below. It is wonderful. After >spending so many years fixated on isopentane in liquid nitrogen and >getting cracked blocks in order to adhere with what was histologically >correct, I have finally moved onto the floating boat technique and my >frozens look nearly as good as paraffin sections. The only difference is >that I use metal floating boats (read: sterilization trays) instead of >plastic petri dishes. This way the blocks cool even quicker - better for >the tissue and morphology - though they still don't shatter. I finally >feel liberated :) > > > >At 9:27 AM -0700 1/4/07, Gayle Callis wrote: >>Yes, your very last suggestion will work perfectly. We place a plastic >>petri dish IN the liquid nitrogen, then you can set the OCT embedded >>spleen in the petri dish. We have shattered spleen and if we do >>isopentane cooled by liquid nitrogen, or direct immersion into liquid >>nitrogen. Just make sure the petri dish is supported so it does not tip >>over in the Liq N2, and do not allow Liq N2 into the dish. You need to >>replenish the liq nitrogen as it is important to maintain the dish >>directly in the N2. In other words, the dish goes canoeing in the Liq N2. >> >>Good luck > > >-- From TJJ <@t> Stowers-Institute.org Thu Jan 4 13:03:41 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Jan 4 13:04:04 2007 Subject: [Histonet] Re: ISH protocol with DGI-labeled probes Message-ID: Andrea, Please tell me what sort of problems you are having, and are you using DIG labeled oligo or riboprobes? We have the Ventana Discovery instrument for our slide-mounted ISH samples. However, we've adapted a benchtop protocol to work with their unit. We primarily use riboprobes and if this is what you are using, I'll be happy to share our protocol with you. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From ASenn <@t> mercy.pmhs.org Thu Jan 4 13:46:39 2007 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Thu Jan 4 13:46:48 2007 Subject: [Histonet] slides 2 Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A8A@pmhs2kxch03> Thank you so very much to all of you that responded to my son's request for microscopic slides!!! You were all very helpful!!! Amy From sjchtascp <@t> yahoo.com Thu Jan 4 15:25:38 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Jan 4 15:25:44 2007 Subject: [Histonet] Histo work wanted Message-ID: <807936.77112.qm@web38210.mail.mud.yahoo.com> Good afternoon everyone, I just finished an assignment in the Milwaukee, WI area. I'm currently seeking any histo position in SoWI or No.Ill. Steve __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From opiecurt <@t> yahoo.com Thu Jan 4 15:36:43 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Thu Jan 4 15:36:51 2007 Subject: [Histonet] H. Pylori control block will be ready tomorrow Message-ID: <831294.55415.qm@web81613.mail.mud.yahoo.com> i know i mentioned that i needed AFB but because i got so many offers i would like to see if someone might have a little extra PCP too. maybe i can get some AFB from john and some PCP from jane? anybody got any extra PCP??? boy, that just doesn't sound right. curt __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From vonavi <@t> inbox.lv Thu Jan 4 15:39:21 2007 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Thu Jan 4 15:39:34 2007 Subject: [Histonet] Technovit 9100 New Message-ID: <1167946761.459d740912bdc@www.inbox.lv> Hello once again, I have a few more questions concerning Technovit 9100 New. For which purpose it is necessary to generate a vacuum around the mould during the polymerization (it is written in the manual)? To get rid of bubbles? To excluded oxygen during the polymerization process? For which purpose it is necessary to have sealed embedding moulds (it is written in the manual)? To prevent evaporation of the polymerization mixture? To excluded oxygen during the polymerization process? Simply, in the manual it is written that no air should be inside the embedding mould. And I cannot understand why it is written. Is adequate polymerization possible in the presence of air oxygen? Thank you! Andrey Ivanov From POWELL_SA <@t> Mercer.edu Thu Jan 4 16:11:55 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Jan 4 16:12:20 2007 Subject: [Histonet] GSH Annual Symposium Message-ID: <01MBJEXZKSVQ8WWKV9@Macon2.Mercer.edu> The Georgia Society for Histotechnology Announces their 2007 Annual Symposium Callaway Gardens, GA April 13-15, 2007 Planned Program Speakers and Topics: Dr. Michael Howard ---- IHC from a Pathologist Perspective Dr. Ana Maria Abreu-Velez ---- Immunofluorescence Dr. Sheriff Zaki of CDC ---- Things We Do Not See In a Regular Histology Lab as a Pathologist Pat Grier HT(ASCP) of CDC---Things We Do Not See In a Regular Histology Lab as a Histotechnologist Dave Reberry & Chris Sheeder of DAKO ---- Various topics on Basic IHC, Target Retrieval, Dilutions, Autostainer Setup, Manual IHC. Also DAKO will do two workshops; one on Autostaining and one on Pascal Antigen Retrieval For final program brochure please contact Jonathan Jones, GSH Education Chair - jjones@gadermpath.com, Mike Ayers, GSH President - lmayers@hughes.net, or Shirley Powell, GSH Secretary - powell_sa@mercer.edu. Vendors please contact our Exhibit Liaison Chris Coley at Chris.Coley@ahss.org for exhibit information. HOTEL INFORMATION: Mountain Creek Inn at Callaway Gardens - Be sure to visit their web site www.callawaygardens.com to appreciate the location and its amenities. Rates are $119 plus tax per room, per night based on single, double, triple, or quad. This reduced rate includes lodging, admission to the Gardens, use of the Fitness Center, in room coffee, newspaper, and telephone access fees will be waived. Rates are good for three days prior to and three days after the conclusion of the meeting. So plan your vacation early. Bring your family to enjoy the Gardens, swimming, golfing, fly fishing, bass fishing, educational tours, tennis, shopping. Visit the beautiful Sibley Horticulture Center, the Callaway Discovery Center, the Pioneer Log Cabin, The Gardens' bike and walking trails, Mr. Cason's Vegetable Garden, the Day Butterfly Center, and the area scenery and flowers. PLEASE MAKE YOUR RESERVATIONS EARLY BY CALLING 1-800-CALLAWAY 225-5292. Be sure to state you are attending the GSH Annual Symposium for the discounted rate. PLEASE NOTE: CUT OFF DATE FOR THIS GREAT RATE IS MARCH 1, 2007 SO CALL NOW FOR RESERVATIONS. From akemiat3377 <@t> yahoo.com Thu Jan 4 17:04:16 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Jan 4 17:04:23 2007 Subject: [Histonet] Cat Hematoxylin Message-ID: <23141.18158.qm@web31306.mail.mud.yahoo.com> Hi Jennifer, Below is info I addressed to the histonet as well as Chuck Churukian. Dear Chuck, There was some talk about CAT hematoxylin in the histonet, so I clarified the info. It was said that you and David developed it. The date was incorrect in my answer. I actually did the R&D in 2000 & released it in 2001. With Love, Akemi Note: forwarded message attached. Forwarded Message [ Download File | Save to Yahoo! Briefcase ] Date: Wed, 3 Jan 2007 09:11:35 -0800 (PST) From: "Akemi Allison-Tacha" Subject: CAT Hematoxylin To: Histonet@lists.utsouthwestern.edu HTML Attachment [ Scan and Save to Computer | Save to Yahoo! Briefcase ] Hello & Happy New Year! Regarding a histonet e-mail question about who developed CAT Hematoxylin. Here is the clarification. There were actually three histologists involved with the development. While I was working at Biocare, Chuck Churukian gave me the modification for Mayer's Hematoxylin, which i in turn made several modifications too. I also came up with the proprietary ingredient for stabalization & David Tacha from Biocare suggested the pH portion. Thus the CAT (Churukian-Allison-Tacha) I started my research in 2001 & officially released it in 2002 at the NSH meeting. David & I developed it to be used for IHC, H&E's & SS. I was the person who actually made-up the hematoxylin from A-Z. & learned alot about bulk manufacturing & pH. Happy New Year, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From lnlj <@t> novonordisk.com Fri Jan 5 04:35:50 2007 From: lnlj <@t> novonordisk.com (LNLJ (Lene Lyngsie Jensen)) Date: Fri Jan 5 04:36:01 2007 Subject: [Histonet] Perfusion fixation of pig lungs In-Reply-To: <459D28B0.7060808@umdnj.edu> Message-ID: <48BB4C2CF37C1843930ECAC01475F835F6F39D@EXDKBA025.corp.novocorp.net> Hi Geoff, Kemlo, Edwards and histonet. Thank you for your contribution. Unfortunately is fixation via the trachea and bronchial tree not an option. We tried it in the initiating studies on rats. Perfusion fixation via the vascular system worked in rats (we did a full body fixation) but they are also significant smaller than a pig. It would be very good if we could get a fixation via the vascular system in pigs as well, but limited to the lungs (because of the body size of the pig). This fixation allows the airways and alveoli to remain I their air filled state. At the same time allows this method fixation of the surface lining layer of alveoli and smaller airways, which is washed away in instillation fixation. The perfusion fixation is much more complicated, because it requires a number of parameters to be controlled, such as the pressure applied to the vessels, the degree of inflation of the air spaces, the transpulmonary pressure, as well as the osmotic and oncotic pressure of the fixative. It would have been excellent if there was some one here that knew how to do it in pigs. I have also thought off (as Geoff also suggested) to limit the perfusion to one lung at the time. To ensure a minimize variation I the fixation from animal to animal. The goal is also to find a fixation way that can work with PFA and fixative used for EM as well. If any one can contribute with some more ideas, please do so. Thanks to Geoff, Kemlo and Edwards. Lene :-) -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: 4. januar 2007 17:18 To: LNLJ (Lene Lyngsie Jensen) Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Perfusion fixation of pig lungs Hi Lene: 1. Decide if you want to perfuse via the vascular system (washing out the blood) or instill fixative via the trachea and bronchial tree. Note that the position of the animal during instillation will determine which regions of the lung are fixed first (or at all!). Study of the anatomy of the bronchial tree of the pig will pay dividends. 2. Decide if you are going to look at the entire lung or just one (or more) small regions. I suggest looking at one bronchopulmonary segment from one (or more?) lobes to conserve resources and minimize variation from animal to animal. If that is your decision then you can do a little dissection and either perfuse that segment or instill into that segment. Good luck! Geoff LNLJ (Lene Lyngsie Jensen) wrote: >Hey. > >I hope that someone here can help me. > >We are planning to do some research in pig lungs. Due to this >investigation it is very important to have a fast and effective >fixation. > >We will therefore try to make a perfusion fixation. But a pig is a very >large animal and therefore we would like to limit the fixation only to >the lung region, and not a full body perfusion fixation. > >Is there anyone how has experience with perfusion fixation limit to only >one organ in pigs? > >All suggestion will be read with enthusiasm. > >Experience from similar fixation methods in other animals is welcome as >well. > >Thanks. >________________________________________________ > >Lene Lyngsie Jensen >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From abright <@t> brightinstruments.com Fri Jan 5 09:36:35 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Jan 5 09:35:41 2007 Subject: [Histonet] Re: Liquid nitrogen, mouse spleen - Freezing tissues Message-ID: Dear Andrea/ Gayle, We have been manufacturing metal moulds for our cryostats and have found the same, the front face of the block had been perfect for the Mohs technique too. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 04 January 2007 18:27 To: Andrea Hooper; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Liquid nitrogen, mouse spleen - Freezing tissues Dear Andrea and all, Hooray for liberation! We will try the metal sterilization trays and what about disposable metal weighing dishes? I think these are out in vendor land too. Will be havet to go shopping. Another key issue with isopentane also know as 2 methyl butane is the storage problem. The "floating boat" method eliminates a rather nasty solvent, although you still need to use Liquid nitrogen with good ventilation. Gayle Callis At 11:01 AM 1/4/2007, you wrote: >I second Gayle's technique described below. It is wonderful. After >spending so many years fixated on isopentane in liquid nitrogen and >getting cracked blocks in order to adhere with what was histologically >correct, I have finally moved onto the floating boat technique and my >frozens look nearly as good as paraffin sections. The only difference is >that I use metal floating boats (read: sterilization trays) instead of >plastic petri dishes. This way the blocks cool even quicker - better for >the tissue and morphology - though they still don't shatter. I finally >feel liberated :) > > > >At 9:27 AM -0700 1/4/07, Gayle Callis wrote: >>Yes, your very last suggestion will work perfectly. We place a >>plastic >>petri dish IN the liquid nitrogen, then you can set the OCT embedded >>spleen in the petri dish. We have shattered spleen and if we do >>isopentane cooled by liquid nitrogen, or direct immersion into liquid >>nitrogen. Just make sure the petri dish is supported so it does not tip >>over in the Liq N2, and do not allow Liq N2 into the dish. You need to >>replenish the liq nitrogen as it is important to maintain the dish >>directly in the N2. In other words, the dish goes canoeing in the Liq N2. >> >>Good luck > > >-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Jan 5 10:23:05 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jan 5 10:23:16 2007 Subject: [Histonet] "GOLD" slides?? In-Reply-To: <5F31F38C96781A4FBE3196EBC22D478004A6EC@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BEA@LSRIEXCH1.lsmaster.lifespan.org> A few weeks back someone recommended "GOLD" slides from Fisher, and said that nothing falls of those slides while staining. I can't find any reference to "GOLD" slides in the Fisher catalog. Could someone provide information on these slides? Thanks. From jlinda <@t> ces.clemson.edu Fri Jan 5 10:35:09 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Jan 5 10:26:10 2007 Subject: [Histonet] RE: Technovit 9100 New Message-ID: <5.2.1.1.2.20070105104950.027b5008@mailhost.ces.clemson.edu> Andrey, Don't you just love the ambiguity of "shorter" versus "longer" time periods? I haven't used the Neu 9100 but, having used the"old" 9100, I have found that I can leave the destabilized monomer in the refrigerator at 4C for a year. I haven't tried freezing, but I would think that would hold indefinitely. The thing to remember is that both frozen and refrigerated solutions should be allowed to come to room temp before using as the solutions will have a condensate on the inside of the jar that doesn't mix well with the monomer. Also, I filter my solutions and reuse as needed;however for the final infiltration and embedding solution, I always use fresh monomer. Any remaining fresh monomer can be filtered and stored as "used"solution and can be used for base layers and "first-step" infiltration solutions thus recycling the solution with little waste. Good Luck! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From barrickstacey <@t> yahoo.com Fri Jan 5 10:41:31 2007 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Fri Jan 5 10:41:42 2007 Subject: [Histonet] "GOLD" slides?? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BEA@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <674660.4302.qm@web54311.mail.yahoo.com> I believe they are referring to the Fisher Superfrost Plus Gold slides. They are supposed to work better than the Reg Superfrost Plus slides. "Monfils, Paul" wrote: A few weeks back someone recommended "GOLD" slides from Fisher, and said that nothing falls of those slides while staining. I can't find any reference to "GOLD" slides in the Fisher catalog. Could someone provide information on these slides? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From PMonfils <@t> Lifespan.org Fri Jan 5 10:42:22 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jan 5 10:42:31 2007 Subject: [Histonet] "GOLD" slides?? - P.S. In-Reply-To: <6D6739C3800D8F4F9AD569452AD482B6027C99CC@usnh0032k08.eriesci.apogent.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BEB@LSRIEXCH1.lsmaster.lifespan.org> Thanks to those who responded. I found the slides on the Fisher website. One further question. The catalog description indicates these slides are for frozen sections. Has anyone used them for paraffin sections, and do they work well for that purpose? Thanks. Paul M. From dfvilleg <@t> mtu.edu Fri Jan 5 10:55:35 2007 From: dfvilleg <@t> mtu.edu (Diego Villegas) Date: Fri Jan 5 10:55:46 2007 Subject: [Histonet] Von Kossa Stain Message-ID: <459E8307.2010707@mtu.edu> Hello Histonetters, I am working in the insertion sites of ligaments. There are two fibrocartilages in the insertion (uncalcified and calcified). I would like to determine the limit between these two zones, but I can't decalcified the samples because I would see no calcium. I could cut samples with a diamond blade but they are really thick (about 100 microns). Actually, I just need to differentiate this two zones. Could I stain these thick samples with Von Kossa stain? Any other method?. Thanks a lot. Diego Villegas Biomechanics Lab Michigan Tech. From Karen.Heckford <@t> CHW.edu Fri Jan 5 10:58:39 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Fri Jan 5 11:00:12 2007 Subject: [Histonet] Carnoy's Message-ID: Hi Everyone, I was wondering if anyone has ever had Carnoy's solution take writing off a tissue cassette. We are using the Secureline pens. Feed back as soon as possible would be great. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From pruegg <@t> ihctech.net Fri Jan 5 11:03:01 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jan 5 11:03:18 2007 Subject: [Histonet] "GOLD" slides?? - P.S. In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BEB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <002b01c730eb$5be65ce0$6501a8c0@Patsy> I have used the "gold" slides for paraffin and GMA plastic sections and they did the job but were too expensive to use routinely. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, January 05, 2007 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "GOLD" slides?? - P.S. Thanks to those who responded. I found the slides on the Fisher website. One further question. The catalog description indicates these slides are for frozen sections. Has anyone used them for paraffin sections, and do they work well for that purpose? Thanks. Paul M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Jan 5 11:08:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jan 5 11:08:44 2007 Subject: [Histonet] "GOLD" slides?? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BEA@LSRIEXCH1.lsmaster. lifespan.org> References: <5F31F38C96781A4FBE3196EBC22D478004A6EC@fhosxchmb006.ADVENTISTCORP.NET> <4EBFF65383B74D49995298C4976D1D5E273BEA@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <6.0.0.22.1.20070105095151.01b0cf80@gemini.msu.montana.edu> Have always been under the impression these are for use with frozen sections as the package insert states (quote): 1. Inherently tissue binding so there is no need for sprays or adhesives with these slides. 2. Allow plus gold slides to contact the forzen sections directly in cryostat using standard technic 3. Place slide horizontally on table top at room temp for 2- 30 seconda for fresh tissue (frozens) or 1 minutes for fixed frozen sections. There was more info here, but the longer the bonding time, the better the frozen sections adhere to slides. 4. Postfix in acetone or alcohol based fixative, postfixation in NBF is not recommended. Stain per a standard protocol. I am not so sure that "nothing" falls off these slides. They are far more expensive than the regular Plus charge, the boxes we have had only 25 slides. These little gold nuggets were approx double the cost of regular plus charge, We have used them with problematic frozen sections, but not for for formalin fixed paraffin embedded (FFPE) sections. There are people who use them for FFPE sections, swear by their holding capabilities - there are replies on Histonet about using them. The only time we can justify the cost is with a really problematic frozen section release and if that is a terrible problem, we go to the Cryojane where the sections do NOT release from the surface. Erie Scientific also developed another coated slide, called Extra for use with EDTA antigen retrieval. Apparently high pH EDTA raises havoc with a glass slide surface, basically chelates ions from the surface, leading to release of sections. If this is a problem you experience you should contact Erie Scientific for specifics. Our fisher cat. no. was 22-035813, which may have changed since we bought the slides. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JMitchell <@t> uwhealth.org Fri Jan 5 11:10:22 2007 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Fri Jan 5 11:10:33 2007 Subject: [Histonet] Muscle Citrate Synthase Analysis Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB10225AC88@uwhis-xchng4.uwhis.hosp.wisc.edu> Does anyone have any experience with citrate synthase mitochondrial enzyme analysis on frozen muscle? I would appreciate any input on how this testing is done. Thanks! Jean Mitchell, BS, HT University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager Madison, WI From BMierow <@t> smdc.org Fri Jan 5 12:07:17 2007 From: BMierow <@t> smdc.org (Mierow, Brett T.) Date: Fri Jan 5 12:08:41 2007 Subject: [Histonet] Job Opening Message-ID: <8514F5095A68704AB5C50BCE5F783D426E5742@SCREECH.ntcampus.smdc.org> Job Posting Title Supervisor-Anatomic Pathology City and State Duluth, MN Department Laboratory Physical Location (building) St. Mary's Medical Center Auto req ID 173BR Type of Employment Regular, full-time FTE 1.00 Shift Days Start time 8:00 a.m. End time 4:30 a.m. Union position? No Job Description JOB SUMMARY: The Anatomic Pathology Supervisor directs operations of the technical sections including Histology, Cytology, Pathology Assistants and the Pathology Office. Responsibilities include; managing workflow, maintaining policy and procedure manuals, staff competency assessment, test utilization, fiscal responsibilities, compliance with regulatory standards, formation and implementation of quality monitors for the section, and human resources management according to SMDC guidelines. Promotes the mission and vision of SMDC. Position requires high level of customer service skills to establish and enhance positive relationships with providers, co-workers and others. QUALIFICATIONS: Graduate of an accredited school of Histotechnology, Ctyotechnology, Clinical Laboratory Scientist or equivalent discipline. Minimum of five years experience in a clinical laboratory. ASCP or equivalent or registry eligible. Monthly Minimum Salary $4,199.87 Job Posting Deadline (Union) 21-Dec-2006 SMDC url: http://www.duluthclinic.org/employment/jobsearchinternal.htm This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From carl.hobbs <@t> kcl.ac.uk Fri Jan 5 12:20:50 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Jan 5 12:21:25 2007 Subject: [Histonet] re: von kossa Message-ID: <001001c730f6$3ae7db30$0201a8c0@carlba65530bda> We use Tripp and Mackay's modification and it works very well when we subsequently decalcify and pwax -processed mouse tissues. However, I don't know what your tissue prep. is.... Carl From jcresor <@t> lcpath.com Fri Jan 5 12:26:37 2007 From: jcresor <@t> lcpath.com (Jennifer Cresor) Date: Fri Jan 5 12:26:46 2007 Subject: [Histonet] Bone Marrow stain Message-ID: <5475dca5024c74419f580ec40a3affe6@mail2.lcpath.com> Hello all, We have been trying to find a bone marrow stain with excellent quality. The pathologists say there is not enough staining of the granules in the myelocytes. We have been experimenting with new stains. We have tried a bone marrow stain from BBC called Bomar stain, and now a stain from Stat Lab called Hema-Diff. The doctors are still not satisfied. Any recommendations would be appreciated. Thank you, Jennifer jcresor@lcpath.com From Janet.Bonner <@t> FLHOSP.ORG Fri Jan 5 13:09:00 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jan 5 13:11:36 2007 Subject: [Histonet] Bone Marrow stain References: <5475dca5024c74419f580ec40a3affe6@mail2.lcpath.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A6FB@fhosxchmb006.ADVENTISTCORP.NET> Jennifer, Perhaps the fixative/decal is the problem? Try BBC Company's RAPID DECAL IMMUNO. We have had wonderful results and the stains done on our bone marrows were greatly improved! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer Cresor Sent: Fri 1/5/2007 1:26 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Marrow stain Hello all, We have been trying to find a bone marrow stain with excellent quality. The pathologists say there is not enough staining of the granules in the myelocytes. We have been experimenting with new stains. We have tried a bone marrow stain from BBC called Bomar stain, and now a stain from Stat Lab called Hema-Diff. The doctors are still not satisfied. Any recommendations would be appreciated. Thank you, Jennifer jcresor@lcpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From LBeitman <@t> cellmarque.com Fri Jan 5 13:11:04 2007 From: LBeitman <@t> cellmarque.com (Liz Beitman) Date: Fri Jan 5 13:12:52 2007 Subject: [Histonet] Career Opportunity Message-ID: <7F2A2AE306CE254DB7279E86A51A740607AD75@CMROCEX01.cellmarque.local> Cell Marque Corp., an innovative and Customer-oriented manufacturer of Immunohistochemistry products, is currently searching for a Histotechnologist to join us at our brand new facility in Rocklin, CA. Successful candidate must have previous laboratory, slide preparation (with knowledge of mictrotomy and proven cutting skills), and H&E staining experience; knowledge of IHC staining is preferred. The position is 7AM to 4PM Monday through Friday. Experience/Minimum Requirement: At least 2 years experience in Histology lab and CAP registered. Interested candidates should send resume to histocareers@cellmarque.com or fax to 916-746-8989. Thank you! From kmerriam2003 <@t> yahoo.com Fri Jan 5 13:13:58 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jan 5 13:14:09 2007 Subject: [Histonet] HOPE fixation Message-ID: <20070105191358.9718.qmail@web50305.mail.yahoo.com> OK, I know someone on this list must know the answer to this. What is HOPE fixation? I am reading a journal article that refers to this procedure. I have done a Pubmed search, and I can find several references to it, but no actual protocol on how to make the stuff up (maybe you need to buy it?). I am not sure if I would ever be trying this method, but inquiring minds want to know! Thanks, Kim Kim Merriam, MA, HT(ASCP) Amgen Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bhewlett <@t> cogeco.ca Fri Jan 5 13:30:50 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Jan 5 13:30:52 2007 Subject: [Histonet] HOPE fixation References: <20070105191358.9718.qmail@web50305.mail.yahoo.com> Message-ID: <003e01c73100$0264ccf0$6500a8c0@mainbox> Kim, HOPE =Hepes-glutamic acid buffer mediated Organic solvent Protection Effect. Tissues are placed in this non-fixative buffer at 4C O/N, then fixed/dehydrated in acetone and transferred to paraffin. Here is a website http://www.hope-fixation.com/ Bryan ----- Original Message ----- From: "Kim Merriam" To: "Histonet" Sent: Friday, January 05, 2007 2:13 PM Subject: [Histonet] HOPE fixation OK, I know someone on this list must know the answer to this. What is HOPE fixation? I am reading a journal article that refers to this procedure. I have done a Pubmed search, and I can find several references to it, but no actual protocol on how to make the stuff up (maybe you need to buy it?). I am not sure if I would ever be trying this method, but inquiring minds want to know! Thanks, Kim Kim Merriam, MA, HT(ASCP) Amgen Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Jan 5 13:35:03 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Jan 5 13:35:11 2007 Subject: [Histonet] HOPE fixation In-Reply-To: <20070105191358.9718.qmail@web50305.mail.yahoo.com> References: <20070105191358.9718.qmail@web50305.mail.yahoo.com> Message-ID: <6.2.5.6.2.20070105143227.01c474b0@vet.upenn.edu> At 02:13 PM 1/5/2007, Kim Merriam wrote: >OK, I know someone on this list must know the answer to this. What >is HOPE fixation? I am reading a journal article that refers to >this procedure. I have done a Pubmed search, and I can find several >references to it, but no actual protocol on how to make the stuff up >(maybe you need to buy it?). > >I am not sure if I would ever be trying this method, but inquiring >minds want to know! > >Thanks, >Kim > >Kim Merriam, MA, HT(ASCP) >Amgen >Cambridge, MA > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim, The product was introduced in Europe a few years ago (3 or 4) and is available there. I am not sure if they have a distributor here or not. It is not widely used at this time and is interesting. I spoke to some people at a meeting there in 2003. I know this is not much help, at least you know where it started and Eurpean journals or connections may be a better bet. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From cwscouten <@t> myneurolab.com Fri Jan 5 13:52:21 2007 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Jan 5 13:51:33 2007 Subject: [Histonet] Perfusion fixation of pig lungs Message-ID: <5784D843593D874C93E9BADCB87342AB027D993B@tpiserver03.Coretech-holdings.com> The fastest (less advance surgery) and most effective perfusion would be whole body under higher than physiological pressure. See the discussion in Microscopy Today, May 2006 issue, for some ideas. You would need about 5 gallons of liquid prewash, and 2 or 3 gallons of fixative, for a quess. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=4 71005&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=S acrifice+Equipment&idsubcategory=21 See the link above for ideas of what is needed, but our fluid tanks are not large enough. You (or we) would rig something similar on some Carboy Tanks. Getting through the heart and applying fluid at 300 mm Hg, the pig will be drained of blood in seconds. Fixative can then be applied and will get there faster, measured from the time the diaphragm or chest cavity is punctured, than any other way I know of. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LNLJ (Lene Lyngsie Jensen) Sent: Thursday, January 04, 2007 4:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Perfusion fixation of pig lungs Hey. I hope that someone here can help me. We are planning to do some research in pig lungs. Due to this investigation it is very important to have a fast and effective fixation. We will therefore try to make a perfusion fixation. But a pig is a very large animal and therefore we would like to limit the fixation only to the lung region, and not a full body perfusion fixation. Is there anyone how has experience with perfusion fixation limit to only one organ in pigs? All suggestion will be read with enthusiasm. Experience from similar fixation methods in other animals is welcome as well. Thanks. ________________________________________________ Lene Lyngsie Jensen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Fri Jan 5 14:13:48 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Jan 5 14:14:06 2007 Subject: [Histonet] hacker microwave Message-ID: <8C8FF1D253DD5FE-17B4-161E@FWM-D33.sysops.aol.com> Anybody out there using the new hacker microwave? I would love some input, we are thinking about getting one. Thanks in advance Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From mtarango <@t> nvcancer.org Fri Jan 5 14:14:55 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Jan 5 14:15:22 2007 Subject: [Histonet] Bone Marrow stain Message-ID: <5AEC610C1CE02945BD63A395BA763EDEE00FB5@NVCIEXCH02.NVCI.org> I think she's looking for a stain for the bone marrow aspirate smears... Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Friday, January 05, 2007 11:09 AM To: Jennifer Cresor; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone Marrow stain Jennifer, Perhaps the fixative/decal is the problem? Try BBC Company's RAPID DECAL IMMUNO. We have had wonderful results and the stains done on our bone marrows were greatly improved! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer Cresor Sent: Fri 1/5/2007 1:26 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Marrow stain Hello all, We have been trying to find a bone marrow stain with excellent quality. The pathologists say there is not enough staining of the granules in the myelocytes. We have been experimenting with new stains. We have tried a bone marrow stain from BBC called Bomar stain, and now a stain from Stat Lab called Hema-Diff. The doctors are still not satisfied. Any recommendations would be appreciated. Thank you, Jennifer jcresor@lcpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. 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If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From tkngflght <@t> yahoo.com Fri Jan 5 14:18:25 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jan 5 14:18:32 2007 Subject: [Histonet] Travel temp openings--and a few good perm jobs, too. Message-ID: <237827.714.qm@web50913.mail.yahoo.com> Hi again-- I hope everyone had a great Christmas and New Years...now we're all back to work! We have several temp openings--please drop us a line or call if you are even a little curious: Temp openings and number of positions to be filled: New York (near New York City) - 3 techs (We help with NY License application) Connecticut - 2 techs North Carolina - 2 techs Kansas - one tech Ohio - one tech These change frequently as we fill positions and receive new contracts. The permanent positions are everywhere: California Colorado Kansas Oklahoma Texas Ohio New York Massachusetts Connecticut New Jersey Florida North Carolina South Carolina and the way the phones have been ringing there will be more!!! Thank you-- Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From funderwood <@t> mcohio.org Fri Jan 5 14:26:51 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Jan 5 14:27:13 2007 Subject: [Histonet] HOPE fixation Message-ID: Depending on the Pathologist doing the grossing, I think it's where you hope fixation will occur. >>> Kim Merriam 1/5/2007 2:13 PM >>> OK, I know someone on this list must know the answer to this. What is HOPE fixation? I am reading a journal article that refers to this procedure. I have done a Pubmed search, and I can find several references to it, but no actual protocol on how to make the stuff up (maybe you need to buy it?). I am not sure if I would ever be trying this method, but inquiring minds want to know! Thanks, Kim Kim Merriam, MA, HT(ASCP) Amgen Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgibbon <@t> qltinc.com Fri Jan 5 14:33:12 2007 From: kgibbon <@t> qltinc.com (Kevin Gibbon) Date: Fri Jan 5 14:33:24 2007 Subject: [Histonet] Perfusion fixation of pig lungs Message-ID: <66A24278845C594C98E5ECB840D7BFB503A0278F@VAN-EXCH-VS1.qltinc.com> I used to fix whole human and rat lungs by removing them from the body and inflating via the trachea, then tie the trachea shut. The fixation is excellent for lung tissue. kevin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles Scouten Sent: Friday, January 05, 2007 11:52 AM To: LNLJ (Lene Lyngsie Jensen); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Perfusion fixation of pig lungs The fastest (less advance surgery) and most effective perfusion would be whole body under higher than physiological pressure. See the discussion in Microscopy Today, May 2006 issue, for some ideas. You would need about 5 gallons of liquid prewash, and 2 or 3 gallons of fixative, for a quess. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=4 71005&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=S acrifice+Equipment&idsubcategory=21 See the link above for ideas of what is needed, but our fluid tanks are not large enough. You (or we) would rig something similar on some Carboy Tanks. Getting through the heart and applying fluid at 300 mm Hg, the pig will be drained of blood in seconds. Fixative can then be applied and will get there faster, measured from the time the diaphragm or chest cavity is punctured, than any other way I know of. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LNLJ (Lene Lyngsie Jensen) Sent: Thursday, January 04, 2007 4:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Perfusion fixation of pig lungs Hey. I hope that someone here can help me. We are planning to do some research in pig lungs. Due to this investigation it is very important to have a fast and effective fixation. We will therefore try to make a perfusion fixation. But a pig is a very large animal and therefore we would like to limit the fixation only to the lung region, and not a full body perfusion fixation. Is there anyone how has experience with perfusion fixation limit to only one organ in pigs? All suggestion will be read with enthusiasm. Experience from similar fixation methods in other animals is welcome as well. Thanks. ________________________________________________ Lene Lyngsie Jensen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. From JWEEMS <@t> sjha.org Fri Jan 5 14:39:29 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jan 5 14:39:50 2007 Subject: [Histonet] HOPE fixation Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2EDD@sjhaexc02.sjha.org> perfect!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fred Underwood Sent: Friday, January 05, 2007 3:27 PM To: histonet@lists.utsouthwestern.edu; kmerriam2003@yahoo.com Subject: Re: [Histonet] HOPE fixation Depending on the Pathologist doing the grossing, I think it's where you hope fixation will occur. >>> Kim Merriam 1/5/2007 2:13 PM >>> OK, I know someone on this list must know the answer to this. What is HOPE fixation? I am reading a journal article that refers to this procedure. I have done a Pubmed search, and I can find several references to it, but no actual protocol on how to make the stuff up (maybe you need to buy it?). I am not sure if I would ever be trying this method, but inquiring minds want to know! Thanks, Kim Kim Merriam, MA, HT(ASCP) Amgen Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bhewlett <@t> cogeco.ca Fri Jan 5 14:40:32 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Jan 5 14:40:34 2007 Subject: [Histonet] HOPE fixation References: Message-ID: <005601c73109$bf500e70$6500a8c0@mainbox> If I could resist the temptation, so should you. Cheap shot Fred, but so true! Bryan ----- Original Message ----- From: "Fred Underwood" To: ; Sent: Friday, January 05, 2007 3:26 PM Subject: Re: [Histonet] HOPE fixation > Depending on the Pathologist doing the grossing, I think it's where you > hope fixation will occur. > >>>> Kim Merriam 1/5/2007 2:13 PM >>> > OK, I know someone on this list must know the answer to this. What is > HOPE fixation? I am reading a journal article that refers to this > procedure. I have done a Pubmed search, and I can find several > references to it, but no actual protocol on how to make the stuff up > (maybe you need to buy it?). > > I am not sure if I would ever be trying this method, but inquiring > minds want to know! > > Thanks, > Kim > > Kim Merriam, MA, HT(ASCP) > Amgen > Cambridge, MA > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWEEMS <@t> sjha.org Fri Jan 5 15:42:25 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jan 5 15:42:46 2007 Subject: [Histonet] HSV Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2EE4@sjhaexc02.sjha.org> We have used combined HSV I & II from Biogenex. It has been on backorder and now may be discontinued. We found an RUO from Biocare - which we want to avoid. We can get I and II separately from DAKO and mix them. What are you all doing? Is there a combined non ASR or RUO somewhere? Have a good weekend and thanks for your help, Joyce Joyce Weems Saint Josephs Hospital of Atlanta Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From meligroc <@t> zgi.com Fri Jan 5 17:41:15 2007 From: meligroc <@t> zgi.com (CRME (Criss Meligro)) Date: Fri Jan 5 17:41:46 2007 Subject: [Histonet] Paraffi Sectioning Tape-transfer System Message-ID: <746E68D5CBB205409B12EC32A61EE401024986F4@ned.zgi.com> Hi...just wondering if any one has used the paraffin sectioning tape-transfer system? Made by Instrumedics Inc. Looks interesting! Thanks Criss Meligro From tissuearray <@t> hotmail.com Fri Jan 5 21:22:42 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Jan 5 21:22:51 2007 Subject: [Histonet] Paraffin Sectioning Tape-transfer System Message-ID: I would not recommend it for paraffin. It compresses the tissue and often leaves it interruptible. If you go to my website: http://www.arrayworkshop.com/introvid.html and watch the video it will show the problems using the tape transfer system with paraffin. I have tried to work with the company and nothing came of it. I assume it works well for frozen sectioning. Thom Jensen Histologist/TMA Technician >From: "CRME (Criss Meligro)" >To: histonet@pathology.swmed.edu >Subject: [Histonet] Paraffi Sectioning Tape-transfer System >Date: Fri, 5 Jan 2007 15:41:15 -0800 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc9-f16.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Fri, 5 >Jan 2007 15:42:27 -0800 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1H2yh2-0005Bc-8s; Fri, 05 Jan >2007 17:41:48 -0600 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1H2ygx-0005Av-U8for >histonet@lists.utsouthwestern.edu; Fri, 05 Jan 2007 17:41:45 -0600 >Received: from pathology.swmed.edu ([129.112.48.201])by swlx167.swmed.edu >with esmtp (Exim 4.44) id 1H2ygt-0003Mh-KNfor >histonet@lists.utsouthwestern.edu; Fri, 05 Jan 2007 17:41:43 -0600 >Received: from swlx160.swmed.edu (199.242.236.160) by >pathology.swmed.eduwith ESMTP (Eudora Internet Mail Server 3.1.5) for >; Fri, 5 Jan 2007 17:27:12 -0600 >Received: from worf.zgi.com ([198.99.85.66])by swlx160.swmed.edu with esmtp >(Exim 4.62)(envelope-from ) id 1H2ygn-0003hc-KNfor >histonet@pathology.swmed.edu; Fri, 05 Jan 2007 17:41:39 -0600 >Received: from emfserver.zgi.com (tumble.zgi.com [192.156.218.10])by >tweek.zgi.com with ESMTP id l05NegTf007533for >; Fri, 5 Jan 2007 15:41:27 -0800 (PST) >Received: from 192.168.154.250 by emfserver.zgi.com with ESMTP (SMTPRelay >(Email Firewall v6.3.0)); Fri, 05 Jan 2007 15:41:16 -0800 >Received: from ned.zgi.com ([192.168.124.237]) by rod.zgi.com withMicrosoft >SMTPSVC(6.0.3790.1830); Fri, 5 Jan 2007 15:41:15 -0800 >X-Message-Info: LsUYwwHHNt3/L5qU5P/AuaHlXO/jSm5yN0ILWFSynP4= >X-Server-Uuid: D57C7F34-D984-418D-BA1F-1A254C523610 >X-MimeOLE: Produced By Microsoft Exchange V6.5 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Paraffi Sectioning >Tape-transfer System >Thread-Index: AccxIv3BUL+cGJW2ShC4iNhZdA6snA== >X-OriginalArrivalTime: 05 Jan 2007 23:41:15.0577 >(UTC)FILETIME=[FD8B6A90:01C73122] >X-WSS-ID: 69803D960VS765150-01-01 >X-Scan-Signature: 2e474a294f441e43f7d68d062016d334 >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 56b1ed4a6b9d2c7c11817481be952b1e >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu > >Hi...just wondering if any one has used the paraffin sectioning >tape-transfer system? Made by Instrumedics Inc. Looks interesting! > >Thanks > >Criss Meligro > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Communicate instantly! Use your Hotmail address to sign into Windows Live Messenger now. http://get.live.com/messenger/overview From RORYSBLUE <@t> aol.com Sat Jan 6 09:13:47 2007 From: RORYSBLUE <@t> aol.com (RORYSBLUE@aol.com) Date: Sat Jan 6 09:14:00 2007 Subject: [Histonet] Job opportunity in the Syracuse/Rochester area Message-ID: I am in the process of developing a dermpath lab in Syracuse with a group of dermatologists in the area. I am looking for someone with experience who could assist with all facets of the design and set-up of the laboratory. Please contact by list or directly at _rorysblue@aol.com_ (mailto:rorysblue@aol.com) . Thank you, Michelle From RSRICHMOND <@t> aol.com Sat Jan 6 13:49:22 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Jan 6 13:49:35 2007 Subject: [Histonet] Re: HOPE fixation Message-ID: Kim Merriam asks about HOPE fixation, and Joyce Weems and Bryan Hewlett reply: Depending on the Pathologist doing the grossing, I think it's where you hope fixation will occur. Hey, I resemble that remark! Seriously, this technique is from Germany and seems to be a research technique - at least, I can't think of a clinical use for it. Note that it's not actually a fixative. See www.hope-fixation.com HEPES is one of the Good buffers (see Good's article in the JBC, 1966) - look it up in a Sigma catalog for more information. Bob Richmond Samurai Pathologist Knoxville TN From RSRICHMOND <@t> aol.com Sat Jan 6 13:56:40 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Jan 6 13:56:52 2007 Subject: [Histonet] Re: Bone Marrow stain Message-ID: Jennifer Cresor (where?) asks about bone marrow staining. If you're staining smears, all the Romanovsky (polychrome) type stains work the same way - Giemsa, Wright, what have you. Smear preparation, time staining, and other details of technique are very important. It's particularly important to avoid even the slightest water contamination of your stock bottle of stain. I expect your pathologists to know these things and to carefully supervise your technique until the results are satisfactory (dream on!) If you're staining tissue sections - I don't know of any Romanovsky stain method that works on formalin fixed tissues. Back in the days when dinosaurs walked the earth, we fixed the tissue in Zenker's or Helly's fixative (contains both mercury and chromium. B-5 doesn't work) and did the old Wolbach Giemsa stain - I can give you more information on that if you're interested. Bob Richmond Samurai Pathologist Knoxville TN From akemiat3377 <@t> yahoo.com Sat Jan 6 14:52:35 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Sat Jan 6 14:52:51 2007 Subject: [Histonet] Tape transfer method Message-ID: <20070106205235.37625.qmail@web31312.mail.mud.yahoo.com> NIH (Dr. Steve Hewitt's) Lab TMA Advanced Technology Center has been using for several years. Upside: Keeps TMA's in-line. Drawback: leaves artifact on IHC. You can inquire with Helen Fedor, TMA Manager at John's Hopkins University e-mail: hfedor@jhmi.edu She has researched several methodologies and is linked to SPORE. Good Luck & Happy New Year, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Speciallizing in Histology, IHC & TMA E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From marjoh3 <@t> telus.net Sun Jan 7 09:20:27 2007 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sun Jan 7 09:20:35 2007 Subject: [Histonet] Staining Procedure For Herpes Virus By Immunohistochemistry Message-ID: <000c01c7326f$5cc4aba0$6401a8c0@VALUED20606295> Happy New Year Histonetters, I am looking for an IHC staining procedure for Herpes virus (type 2). Is there anyone out there that would be willing to share their staining procedure along with the reagent list? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada From rjbuesa <@t> yahoo.com Sun Jan 7 10:08:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 7 10:09:02 2007 Subject: [Histonet] Staining Procedure For Herpes Virus By Immunohistochemistry In-Reply-To: <000c01c7326f$5cc4aba0$6401a8c0@VALUED20606295> Message-ID: <548283.35468.qm@web61217.mail.yahoo.com> Marilyn: HSV I&II has been used for many years already in IHC. DAKO manufactures a Monoclonal antibody. I used at 1:200 dilution with HIER at pH6 and standard detecting systems (DAKO). Control is a positive case. Hope this will help you! Ren? J. Marilyn Johnson wrote: Happy New Year Histonetters, I am looking for an IHC staining procedure for Herpes virus (type 2). Is there anyone out there that would be willing to share their staining procedure along with the reagent list? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jnocito <@t> satx.rr.com Sun Jan 7 11:32:38 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Jan 7 11:34:29 2007 Subject: [Histonet] Florida Laws Message-ID: <001401c73281$d426efc0$31614542@yourxhtr8hvc4p> Howdy everyone, I know that Florida changed some of the laws for HTs & HTLs as far as accepting ASCP. How about for PAs? Can anyone email me or give someone to contact as how one applies for a FL license as far as a supervisor or PA? I might have a job opportunity coming up. Joe From spirowg <@t> aol.com Sun Jan 7 11:48:57 2007 From: spirowg <@t> aol.com (spirowg@aol.com) Date: Sun Jan 7 11:49:13 2007 Subject: [Histonet] Spiro Galanis is looking for a lab to train or work. Message-ID: <8C9009B3D4D3E8B-8E8-80D@WEBMAIL-RA05.sysops.aol.com> Hello, I live in the Chicago suburbs and I am looking for a lab to get trained as a Histotechnologist or work. I have a BA in biology with 6 months experience working in a pathology lab. You can contact me at 224-629-3910 and my email is Spirowg@aol.com. Thank you, Spiro ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From ancillarypath <@t> mac.com Sun Jan 7 12:56:05 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Sun Jan 7 12:56:16 2007 Subject: [Histonet] Job Opportunity in IHC/FISH In-Reply-To: <200701071811.l07IBEUt011805@mac.com> References: <200701071811.l07IBEUt011805@mac.com> Message-ID: Dear colleagues, My name is Hadi Yaziji and we have a speciality laboratory based in Miami, FL. We specialize in IHC, CISH, and FISH testing. We have a need for a additional senior-level/supervisory position available immediately. Requirements: 1. Sufficient experience in IHC and/or CISH-FISH to conduct complex testing (multiple stains, automated procedures, manual procedures, sufficient knowledge in QA/QC, excellent knowledge in troubleshooting). 2. Adequate personal skills, good phone manners and computer skills, adequate handling of the English language (Spanish is always a plus here in Miami) 3. Good team player. 4. Qualifications: HT (ASCP) or HTL (ASCP); must have either finished or be qualified for the QIHC exam. MT degree is also accepted. Job and benefits description: 1. Salary: Well compensated, commensurate to the experience level 2. Flexible hours (we actually prefer that you have flexible hours) 3. 4 weeks of vacation/year 4. Health benefits 5. Retirement benefits 6. Extremely exciting and challenging working environment, with research and marketing opportunities as well. Interested colleagues: please email me privately with your resume and a list of 3 references at your earliest convenience. Best regards, ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com From liz <@t> premierlab.com Sun Jan 7 14:35:43 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sun Jan 7 14:26:44 2007 Subject: [Histonet] method for alkaline phosphatase with indoxyl-tetrazolium Message-ID: <000001c7329b$6711fbe0$0d00a8c0@domain.Premier> Hello All I hope everyone out in histoland had a Happy New Year. I have been asked to perform alkaline phosphatase staining of mouse muscle samples from a hindlimb ischemia model The method that they are specifically looking for is the indoxyl-tetrazolium method. Most of the publications that I have reviewed so far just state the method used but not the actually protocol. If this method is used properly it should stain capillary endothelial cells dark blue if they are viable. I have only been able to find one method and that is from the Journal of Histochemistry and Cytochemistry in the technical note: Indoxyl-tetranitro Blue Tetrazolium Method for detection of alkaline phosphatase in Immunohistochemistry. Am I missing something? I could be completely clueless is the method in Sheehan "indoxyl method for alkaline phosphatase" the one I should be using? It has been too many years since I have done any enzyme histochemistry. Any advice would be helpful and thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From sterntaler3 <@t> hotmail.com Sun Jan 7 23:54:46 2007 From: sterntaler3 <@t> hotmail.com (gloria @home) Date: Sun Jan 7 23:55:02 2007 Subject: [Histonet] (no subject) Message-ID: Hi gurus, I would like to perform whole-mount immunohistochemistry on mouse/rat embryos. However, they are bigger than usual. ~E15. Does anyone have a detailed protocol of the typical tools to use solutions, etc., or any particular modifications to be made to already existing protocols? Bearing in mind that they might be too big to mount onto a normal slide, or even a standard depression slide, and there might be a problem with visualization with confocal. Thanks, powerhungrymice _________________________________________________________________ Find singles online in your area with MSN Dating and Match.com! http://cp.intl.match.com/eng/msn/msnsg/wbc/wbc.html From sterntaler3 <@t> hotmail.com Sun Jan 7 23:56:01 2007 From: sterntaler3 <@t> hotmail.com (gloria @home) Date: Sun Jan 7 23:56:15 2007 Subject: [Histonet] big tissue whole mount immunohistochem Message-ID: > >Hi gurus, > >I would like to perform whole-mount immunohistochemistry on mouse/rat >embryos. However, they are bigger than usual. ~E15. > >Does anyone have a detailed protocol of the typical tools to use solutions, >etc., or any particular modifications to be made to already existing >protocols? > >Bearing in mind that they might be too big to mount onto a normal slide, or >even a standard depression slide, and there might be a problem with >visualization with confocal. > >Thanks, > >powerhungrymice > >_________________________________________________________________ >Find singles online in your area with MSN Dating and Match.com! >http://cp.intl.match.com/eng/msn/msnsg/wbc/wbc.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get an advanced look at the new version of Windows Live Messenger. http://get.live.com/messenger/overview From Susan.Walzer <@t> HCAHealthcare.com Mon Jan 8 03:12:12 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Jan 8 03:12:24 2007 Subject: [Histonet] Job Opening Message-ID: <471953BC63077941B82C26A4338272B42F04CD@ORLEV03.hca.corpad.net> We have an opening for a histo-tech at St Pete Gen.Hosp. in St Pete., FL . You can apply online: http://www.stpetegeneralhospital.com/ From settembr <@t> umdnj.edu Mon Jan 8 08:26:15 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jan 8 08:27:35 2007 Subject: [Histonet] Staining Procedure For Herpes Virus By Immunohistochemistry Message-ID: Hello Marilyn, I use Dako's Polyclonal Rabbit Anti-Herpes Simplex Virus Type 2 on FFPE human tissue. Catalog # B 0116 It is an IVD; I use it at 1:300 I use NO pretreatment at all. I use Dako's detection kit LSAB2 and their DAB kit as well and get nice results. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Marilyn Johnson 01/07/07 10:20 AM >>> Happy New Year Histonetters, I am looking for an IHC staining procedure for Herpes virus (type 2). Is there anyone out there that would be willing to share their staining procedure along with the reagent list? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tdobersztyn <@t> chmca.org Mon Jan 8 08:31:59 2007 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Mon Jan 8 08:34:59 2007 Subject: [Histonet] Shandon autosharp In-Reply-To: <20070106180237.6A6A15779@interceptor.chmca.org> Message-ID: Hello all! Just wondering if anyone out there has a Shandon Autosharp 5 for sale? I have use for one, and would greatly appreciate any info/offers. Thank you in advance! Theresa Theresa R Dobersztyn BA, HT (ASCP) Electron Microscopy/Histology Labs Senior Technologist Department of Pathology Akron Children's Hospital 1 Perkins Square Akron, Ohio 44308 330-543-8279 From nwilliams <@t> unipathllc.com Mon Jan 8 08:42:13 2007 From: nwilliams <@t> unipathllc.com (Nancy Williams) Date: Mon Jan 8 08:42:21 2007 Subject: [Histonet] information on urine cell blocks Message-ID: <18334A665430264780053F077275738E60E516@exchange.unipathllc.corp> I asked our cytology technical manager about the cell blocks and this is what she had to say: Hello Histonetters: Any information on doing cell blocks on urines would be appreciated. Specific questions include: 1) Can they be made on cytolyte fixed specimens? Yes - if enough cells are present 2) What volume of specimen do they require? It depends on the cellularity of the specimen - a visible button after centrifuge is optimal - you can try adding agar if no visible button is present, but this is likely to yield an unsatisfactory CB - in other words, you probably shouldn't do a cell block on these specimens 3) Are they reviewed by the cytologist, or do they go straight to pathologist? Both ways are practiced - depends on the preference of the pathologist 4) Are they reimbursable as an 88305 in addition to the cytology concentrated (Thin-Prep)88112 code? Not sure 5) Are they difficult to read? no 6) Are they helpful? Seldom helpful - but can be used for special stains in some cases 7) How well do they correlate to thin-preps? In my very limited experience they are representative of what's on the TP, but they are rarely done because they seldom add any information and it is difficult to justify the time and cost Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 Nancy Williams HT(ASCP) Technical Manager Histology Unipath Denver, Co phone: 303-512-2243 fax:: 303-512- From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Jan 8 08:58:56 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jan 8 08:59:13 2007 Subject: [Histonet] information on urine cell blocks Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012470D4@wahtntex2.waht.swest.nhs.uk> I concur with what Nancy says; unless you have the requirement for wanting multiple sections for special stains then ThinPrep is the way to go. Urines, IMHO, have never been very successfully Cytospun and the amount of work to produce an inferior cell block, suboptimaly stained with reduced resolution makes it pointless unless, as I have said, you want loads of sections and don't have enough sample for multiple ThinPreps. However, if you have a cancer that is shedding loads of cells that is another issue; I've seen clumps of malignant cells in such quantities that you have sufficient material. Should urines go to a Pathologist? Interesting question..... Is an acellular specimen adequate? Given that many types of urine from males will be then I guess not; why should a Pathologist look at nothing to say 'I don't see any malignant cells'? Surely a 'normal' acellular specimen ought to be reported by a CytoTech/ BMS? That obviously involves those that contain rbc's; but even then if the term microhaematuria is used then why send to a Pathologist? You don't send Dipsticks to a Doctor cos they detect blood do you? You send the Patient! Urines are a bit like sputa; are they 'diagnostic' or screening? Why would an MD want to look at loads of acellular urines and negative sputa? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com It's not what we eat but what we digest that makes us strong; not what we gain but what we save that makes us rich; not what we read but what we remember that makes us learned; and not what we profess but what we practice that gives us integrity. --Francis Bacon This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From doug <@t> ppspath.com Mon Jan 8 09:02:23 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Jan 8 08:59:46 2007 Subject: [Histonet] Job Description For Grossing Person Message-ID: Does anyone have a Job Description that they could share for a Grossing Tech? Not a grossing HT just Grossing Tech. Thanks!! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Rcartun <@t> harthosp.org Mon Jan 8 09:05:11 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jan 8 09:05:48 2007 Subject: [Histonet] HSV In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2EE4@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2EE4@sjhaexc02.sjha.org> Message-ID: <45A217570200007700003A31@hcnwgwds01.hh.chs> Hi Joyce: We been using an "HSV cocktail" for years with excellent results. We make a mixture of HSV-1 (B0114) and HSV-2 (B0116) from Dako at 1:1,000 each and incubate for 30' with Dako's EnVision+/AEC+ detection. We do not use antigen retrieval. I'm not sure you really need HSV-2 since HSV-1 will cross-react with HSV-II. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Weems, Joyce" 01/05/07 4:42 PM >>> We have used combined HSV I & II from Biogenex. It has been on backorder and now may be discontinued. We found an RUO from Biocare - which we want to avoid. We can get I and II separately from DAKO and mix them. What are you all doing? Is there a combined non ASR or RUO somewhere? Have a good weekend and thanks for your help, Joyce Joyce Weems Saint Josephs Hospital of Atlanta Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Mon Jan 8 09:05:49 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 8 09:05:56 2007 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <197600.12183.qm@web61222.mail.yahoo.com> Gloria: The only thing is that you will have to end doing the procedure manually and your only concern would be to make sure to use enough reagents to cover the whole section. At the end you just will have a more expensive procedure, but other than that you could complete it using any standard protocol, from the time and dilutions points of view. Ren? J. "gloria @home" wrote: Hi gurus, I would like to perform whole-mount immunohistochemistry on mouse/rat embryos. However, they are bigger than usual. ~E15. Does anyone have a detailed protocol of the typical tools to use solutions, etc., or any particular modifications to be made to already existing protocols? Bearing in mind that they might be too big to mount onto a normal slide, or even a standard depression slide, and there might be a problem with visualization with confocal. Thanks, powerhungrymice _________________________________________________________________ Find singles online in your area with MSN Dating and Match.com! http://cp.intl.match.com/eng/msn/msnsg/wbc/wbc.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From M.Walker <@t> hrsu.mrc.ac.uk Mon Jan 8 09:05:58 2007 From: M.Walker <@t> hrsu.mrc.ac.uk (Marion Walker) Date: Mon Jan 8 09:05:57 2007 Subject: [Histonet] c-kit In-Reply-To: Message-ID: <5EF1494538AC184E86844A6886D9843001272AAE@mailserv.hrsu.mrc.ac.uk> Dear all, I am looking for a c-Kit/CD117 antibody that works on FFPE Rat testis. I have spent most of this morning on the web trying to find something suitable without any luck. If anyone has any suggestions I would be very glad to hear them. Marion Walker MRC Human Reproductive Sciences Unit Edinburgh Bonnie Scotland From gcallis <@t> montana.edu Mon Jan 8 09:34:39 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jan 8 09:34:51 2007 Subject: [Histonet] Paraffi Sectioning Tape-transfer System In-Reply-To: <746E68D5CBB205409B12EC32A61EE401024986F4@ned.zgi.com> References: <746E68D5CBB205409B12EC32A61EE401024986F4@ned.zgi.com> Message-ID: <6.0.0.22.1.20070108083212.01b3e390@gemini.msu.montana.edu> Linda Jenkins ( jlinda@ces.clemson.edu) does this very successfully, but you need to perform the technic in a special way for paraffin. And yes, it does work. Contact Linda for details. At 04:41 PM 1/5/2007, you wrote: >Hi...just wondering if any one has used the paraffin sectioning >tape-transfer system? Made by Instrumedics Inc. Looks interesting! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From mcauliff <@t> umdnj.edu Mon Jan 8 09:36:17 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jan 8 09:35:32 2007 Subject: [Histonet] decalcification of Zebrafish Message-ID: <45A264F1.4000904@umdnj.edu> Dear colleeagues: Anyone have a protocol for the decalcification of adult zebrafish? Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From akbitting <@t> geisinger.edu Mon Jan 8 10:29:08 2007 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jan 8 10:29:31 2007 Subject: [Histonet] CD10 protocol Message-ID: <45A22B04020000C9000041E9@GHSGWIANW5V.GEISINGER.EDU> Does anyone have a good protocol for running Ventana's CD10 on the Benchmark? Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Malcolm.McCallum <@t> tamut.edu Mon Jan 8 10:41:42 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Mon Jan 8 10:43:10 2007 Subject: [Histonet] histology article in this issue of HCB References: <18334A665430264780053F077275738E60E516@exchange.unipathllc.corp> Message-ID: As the editor of Herpetological Conservation and Biology I am pleased to announce that Issue 2 of the first volume has been released. There is a paper on reproductive histology in cottonmouth for the histoherp folks! You can now access the open-access journal online at http://herpconbio.org. The issue has nine research articles, an introductory piece and a complimentary of copy of Scott and Campbell 1982. The next issue will be released in Early April. Articles can be downloaded for free from the journal website. If you are interested in submitting an article to HCB, please download the instructions for authors from the journal website (or request them from me at malcolm.mccallum@herpconbio.org). HCB is a partnership between the World Congress of Herpetology and Partners in Amphibian and Reptile Conservation. We welcome submissions written in English from any country. We are especially intersted in increasing the international involvement in the journal. HCB will not become eligible for inclusion in ISI Citation Reports until 2008, until then the journal staff will self-calculate its citation rating (a.k.a Journal Impact Factor). At the current time, HCB's self-calculated citation rating (JIF) is at least 0.818. There are no page charges or other fees to authors who publish their work in Herpetological Conservation and Biology. Have a nice day, and I hope you enjoy issue 2. VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html From Vickroy.Jim <@t> mhsil.com Mon Jan 8 10:51:18 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Jan 8 10:53:46 2007 Subject: [Histonet] CD4 Message-ID: ONE OF OUR PATHOLOGISTS KEEPS BEGGING US TO ADD A CD4 IMMUNO STAIN. I CAN'T SEEM TO FIND A CD4 THAT IS NOT RUO OR ASR. DOES ANYONE HAVE ANY SUGGESTIONS? ALSO FOR THOSE THAT ARE USING THE RUO OR ASR, HOW HAVE YOU VALIDATED THE USE OF THIS ANTIBODY. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From dahui_you <@t> yahoo.com Mon Jan 8 11:03:22 2007 From: dahui_you <@t> yahoo.com (Dahui You) Date: Mon Jan 8 11:03:50 2007 Subject: [Histonet] Normal mouse sera Message-ID: <20070108170322.38154.qmail@web35703.mail.mud.yahoo.com> Hi, Histonetters: Need you guys' help again! I need to buy normal mouse sera as blocking agent in fluoresence staining. Do you guys know which company offer qualified mouse serum? Thanks a lot. Have a great day! Dahui You Department of Biological Sciences Louisiana State University Baton Rouge, LA 70803 Tel:225-578-5960 Fax:225-578-2594 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From juan.gutierrez <@t> christushealth.org Mon Jan 8 11:08:27 2007 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Mon Jan 8 11:08:38 2007 Subject: [Histonet] CD10 protocol References: <45A22B04020000C9000041E9@GHSGWIANW5V.GEISINGER.EDU> Message-ID: Hi Angela! For the regular Benchmark we do: standard CC1, primary for 16 minutes and select amplify. For the XT and LT we do: standard CC1, 36 min incubation at 42 degrees and amplify. I don't know why we have such a difference in the incubation times, but the end result looks very similar. Hope this helps. Juan C. Gutierrez, HT(ASCP) Histology Supervisor (210)704-2533 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, January 08, 2007 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD10 protocol Does anyone have a good protocol for running Ventana's CD10 on the Benchmark? Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Mon Jan 8 11:27:50 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Jan 8 11:27:52 2007 Subject: [Histonet] looking for a histotech position in the Chicago area Message-ID: <63B8B599DE283148B92E83C78B32C15D041486DA@cmhexbe2.childrensmemorial.org> Hello, I hope everyone had a great Christmas and New Years...now we're all back to work! One of my friends, who are living in the Chicago area, is looking for a histotech position. If any of you know about available one in the hospital or any small medical office, please let me know. We will very appreciate your help. Thank you-- Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From srishan <@t> mail.holyname.org Mon Jan 8 11:34:44 2007 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Mon Jan 8 11:35:07 2007 Subject: [Histonet] memorandum markers Message-ID: Hi, Happy New Year to all. I am looking to order memorandum markers, the dividers used in the slide file cabinets. Any one knows where I could order it? Thanks in advance Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck, NJ 07666 From tim.morken <@t> thermofisher.com Mon Jan 8 11:45:49 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Jan 8 11:46:18 2007 Subject: [Histonet] IHC QC Postion open at Lab Vision, Fremont CA Message-ID: Here is an open position at Lab Vision Corp (part of ThermoFisher Scientific). Please send resumes to me at the email address below. Please note that preference will be given to local candidates (California). ************************************************************************ ************************************** Quality Control Research Associate II Summary: Performs quality control tests on antibodies, detection systems, and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. May oversee work of QC Research Associate I. Major Responsibilities: Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs product IHC stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with Lab Vision/NeoMarkers' Quality Policies and Quality Procedures. Able to work closely with other departments in reaching company goals. Education and/or certification requirements: BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. Certification as Histotechnologist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Reponsibilities May oversee work of QC Research Associate I. Lab Vision, part of ThermoFisher Scientific, manufactures antibodies and automated immunohistochemistry staining platforms for the clinical and research histology laboratory. Lab Vision is located in Fremont, California in the heart of the San Francisco Bay area biotechnology industry. ************************************************************************ *************************** Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 tpmorken@labvision.com or Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From gcallis <@t> montana.edu Mon Jan 8 11:47:47 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jan 8 11:47:58 2007 Subject: Sources of Re: [Histonet] Normal mouse sera In-Reply-To: <20070108170322.38154.qmail@web35703.mail.mud.yahoo.com> References: <20070108170322.38154.qmail@web35703.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20070108104049.01b51c08@gemini.msu.montana.edu> VWR is the cheapest source we found for all species, including mouse serum 100 ml for ~$82, Cat # 14231-816. We realiquote into sterile tubes and stored at -27C until needed. Vector sells their mouse serum (20 ml) about an inexpensively as anyone. Jackson ImmunoResearch sells lyophilized mouse serum in 10ml size, a bit more expensive than Vector but in the dry, sealed, lyophilized stays good for years. All these vendors have the least expensive shipping for us. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From rjbuesa <@t> yahoo.com Mon Jan 8 12:22:09 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 8 12:22:16 2007 Subject: [Histonet] memorandum markers In-Reply-To: Message-ID: <20070108182209.2894.qmail@web61218.mail.yahoo.com> I used to prepare them myself using 3x5 reference cards, cut to measure. A "productive" way of using some spare time (or by son auxiliary personnel). Ren? J. srishan@mail.holyname.org wrote: Hi, Happy New Year to all. I am looking to order memorandum markers, the dividers used in the slide file cabinets. Any one knows where I could order it? Thanks in advance Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck, NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From JWEEMS <@t> sjha.org Mon Jan 8 13:17:17 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jan 8 13:17:36 2007 Subject: [Histonet] Parathyroid Storage Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F0E@sjhaexc02.sjha.org> I am in search of a facility that will store parathyroids. Our neighbor hospital has known of two locations but they are closing and will not accept the specimen that is scheduled to be removed in tomorrow's surgery. Any help (STAT!) would be much appreciated. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pwg1 <@t> cdc.gov Mon Jan 8 13:52:31 2007 From: pwg1 <@t> cdc.gov (Greer, Patricia (CDC/CCID/NCZVED)) Date: Mon Jan 8 13:52:57 2007 Subject: [Histonet] memorandum markers Message-ID: We go to a local print shop and ask them to cut card stock at 3 1/4 X 1 inch (the slide drawers had aren't very deep - you may want 3 1/2 inch length). We get about 4000 at a time and they are much cheaper that ordering them from some of the lab supplies. Pat Greer Infectious Disease Pathology Branch Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, January 08, 2007 1:22 PM To: srishan@mail.holyname.org; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] memorandum markers I used to prepare them myself using 3x5 reference cards, cut to measure. A "productive" way of using some spare time (or by son auxiliary personnel). Ren? J. srishan@mail.holyname.org wrote: Hi, Happy New Year to all. I am looking to order memorandum markers, the dividers used in the slide file cabinets. Any one knows where I could order it? Thanks in advance Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck, NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Jan 8 14:35:54 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jan 8 14:36:10 2007 Subject: [Histonet] memorandum markers In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F56@EMAIL.archildrens.org> You can order them from LabStorage Systems. 1.800.345.4167 Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, January 08, 2007 11:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] memorandum markers Hi, Happy New Year to all. I am looking to order memorandum markers, the dividers used in the slide file cabinets. Any one knows where I could order it? Thanks in advance Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck, NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From hornd <@t> uthscsa.edu Mon Jan 8 14:41:58 2007 From: hornd <@t> uthscsa.edu (Horn, Diane A) Date: Mon Jan 8 14:42:05 2007 Subject: [Histonet] F4/80 in FFPE mouse tissues Message-ID: <819189266B78D543A02D9D3956EE4F6C0484AC2D@addax.win.uthscsa.edu> I am searching for some advice on IHC with F4/80 on mouse tissues. The antibody is from Serotec diluted 1:50 in 2% BSA/PBS. The kidney tissues have been fixed in 4% paraformaldehyde and the liver has been fixed in 10% NFB. The liver stains fine but the kidney has high background and no positive staining for F4/80. I am using a kit from Biocare (rat-on-mouse) that is a biotin free system. The only other thing is the kidney sections were cut about 1-2 months ago and stored in a slide box in the -20 freezer (not frost free). Any advice or protocol would be appreciated! Thanks to all, Diane Diane A. Horn Department of Pathology UTHSCSA 7703 Floyd Curl Dr. San Antonio, TX 78229 210-567-4039 From eileen_dusek <@t> yahoo.com Mon Jan 8 15:18:12 2007 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Mon Jan 8 15:18:21 2007 Subject: [Histonet] Special stain on fixed cytology smear Message-ID: <20070108211812.11717.qmail@web50602.mail.yahoo.com> Hi Everyone, Happiest of New Year A quick cytology question. Can Pneumocysytis and GMS be stained on an alcohol fixed smear? I appreciate your help Eileen Dusek __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From AnthonyH <@t> chw.edu.au Mon Jan 8 15:32:42 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jan 8 15:34:40 2007 Subject: [Histonet] Special stain on fixed cytology smear Message-ID: Yep, Sure can Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen dusek Sent: Tuesday, 9 January 2007 8:18 AM To: histonet Cc: me Subject: [Histonet] Special stain on fixed cytology smear Hi Everyone, Happiest of New Year A quick cytology question. Can Pneumocysytis and GMS be stained on an alcohol fixed smear? I appreciate your help Eileen Dusek __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JWEEMS <@t> sjha.org Mon Jan 8 15:36:06 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jan 8 15:36:49 2007 Subject: [Histonet] Special stain on fixed cytology smear Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F1C@sjhaexc02.sjha.org> Yes - they work fine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of eileen dusek Sent: Monday, January 08, 2007 4:18 PM To: histonet Cc: me Subject: [Histonet] Special stain on fixed cytology smear Hi Everyone, Happiest of New Year A quick cytology question. Can Pneumocysytis and GMS be stained on an alcohol fixed smear? I appreciate your help Eileen Dusek __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From doug <@t> ppspath.com Mon Jan 8 15:39:57 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Jan 8 15:37:20 2007 Subject: [Histonet] Special stain on fixed cytology smear In-Reply-To: <20070108211812.11717.qmail@web50602.mail.yahoo.com> Message-ID: Yes it can. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen dusek Sent: Monday, January 08, 2007 4:18 PM To: histonet Cc: me Subject: [Histonet] Special stain on fixed cytology smear Hi Everyone, Happiest of New Year A quick cytology question. Can Pneumocysytis and GMS be stained on an alcohol fixed smear? I appreciate your help Eileen Dusek __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Mon Jan 8 16:51:51 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Jan 8 16:52:13 2007 Subject: [Histonet] Sheep CD31 Message-ID: Does anyone know of an anti-CD31 that works on sheep FFPE tissues? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From akemiat3377 <@t> yahoo.com Mon Jan 8 17:02:33 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Jan 8 17:02:43 2007 Subject: [Histonet] RUO OR ASR Message-ID: <255459.23548.qm@web31304.mail.mud.yahoo.com> Hi All, I hate to show my stupidity, but I must be a little out of the loop on these. I know that RUO means research use only, but what does ASR mean? Don't shoot me down in flames guys! Sincerely, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology & TMA Madison, WI E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From JWEEMS <@t> sjha.org Mon Jan 8 17:08:39 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jan 8 17:09:09 2007 Subject: [Histonet] RUO OR ASR Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F24@sjhaexc02.sjha.org> Analyte Specific Reagent - not approve by FDA... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi Allison-Tacha Sent: Monday, January 08, 2007 6:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RUO OR ASR Hi All, I hate to show my stupidity, but I must be a little out of the loop on these. I know that RUO means research use only, but what does ASR mean? Don't shoot me down in flames guys! Sincerely, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology & TMA Madison, WI E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From tim.morken <@t> thermofisher.com Mon Jan 8 17:22:14 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Jan 8 17:22:42 2007 Subject: [Histonet] RUO OR ASR In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F24@sjhaexc02.sjha.org> Message-ID: Joyce, Just to clarify, when you say the ASR is " not approved by FDA" you make it seem they cannot be used by a diagnostic lab. But the ASR designation was specifically implemented to allow diagnostic labs to use antibodies that had not been fully validated by the company that sells it and puts the onus of validation on the lab that uses it. The company must be FDA-registered to sell ASR's and only CLIA-approved diagnostic labs can use ASR's as diagnostic devices. The ASR does require a notice that it is not FDA approved, but the lab can also attach a notice that ASR's do not require FDA approval. Tim Morken Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, January 08, 2007 3:09 PM To: Akemi Allison-Tacha; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RUO OR ASR Analyte Specific Reagent - not approve by FDA... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi Allison-Tacha Sent: Monday, January 08, 2007 6:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RUO OR ASR Hi All, I hate to show my stupidity, but I must be a little out of the loop on these. I know that RUO means research use only, but what does ASR mean? Don't shoot me down in flames guys! Sincerely, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology & TMA Madison, WI E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Mon Jan 8 17:32:44 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jan 8 17:33:12 2007 Subject: [Histonet] RUO OR ASR Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F25@sjhaexc02.sjha.org> Thanks Tim! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim Sent: Monday, January 08, 2007 6:22 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RUO OR ASR Joyce, Just to clarify, when you say the ASR is " not approved by FDA" you make it seem they cannot be used by a diagnostic lab. But the ASR designation was specifically implemented to allow diagnostic labs to use antibodies that had not been fully validated by the company that sells it and puts the onus of validation on the lab that uses it. The company must be FDA-registered to sell ASR's and only CLIA-approved diagnostic labs can use ASR's as diagnostic devices. The ASR does require a notice that it is not FDA approved, but the lab can also attach a notice that ASR's do not require FDA approval. Tim Morken Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, January 08, 2007 3:09 PM To: Akemi Allison-Tacha; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RUO OR ASR Analyte Specific Reagent - not approve by FDA... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi Allison-Tacha Sent: Monday, January 08, 2007 6:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RUO OR ASR Hi All, I hate to show my stupidity, but I must be a little out of the loop on these. I know that RUO means research use only, but what does ASR mean? Don't shoot me down in flames guys! Sincerely, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology & TMA Madison, WI E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Mon Jan 8 17:40:21 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jan 8 17:40:37 2007 Subject: [Histonet] Sheep CD31 In-Reply-To: References: Message-ID: <6.0.0.22.1.20070108163815.01b3cd18@gemini.msu.montana.edu> Serotec veterinary antibody chart indicates CO-31D4 should work, check with their technical services to make sure this is true. This is their number, Cat # or clone ???? t 03:51 PM 1/8/2007, you wrote: >Does anyone know of an anti-CD31 that works on sheep FFPE tissues? >Thanks, >Albert > >Albert C. Grobe, PhD >International Heart Institute of Montana Foundation >Tissue Engineering Lab, Saint Patrick Hospital Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From mbmphoto <@t> gmail.com Tue Jan 9 00:37:21 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Jan 9 00:35:57 2007 Subject: [Histonet] need protocol for Neu N IHC stain Message-ID: <085A3F3A-F870-4C92-93ED-DA584B575EB8@gmail.com> Hello, Please, could someone provide a Neu N (neuron specific marker) IFA IHC protocol? I need to stain 40um cryostat free-floating primate fixed sections using this marker. I would welcome any suggestions from vendors too. I look forward to hearing from you. Maria Bartola Mejia UCSF Department of Neurosurgery San Francisco, CA From RJLevier <@t> LancasterGeneral.org Tue Jan 9 07:08:24 2007 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Tue Jan 9 07:08:33 2007 Subject: [Histonet] Processing with Clear-Rite 3 Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2019E37C7@MAIL-LR.lha.org> Please help.....If you are using Clear-Rite 3 and also processing on a shorter biopsy schedule, would you please share the times you are using at each station? Thanks in advance for all your help! Rebecca J. LeVier HT(ASCP) Histology Supervisor Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sterntaler3 <@t> hotmail.com Tue Jan 9 07:35:01 2007 From: sterntaler3 <@t> hotmail.com (gloria @home) Date: Tue Jan 9 07:35:17 2007 Subject: [Histonet] Microinjection whole-mount immunolabelling Message-ID: Hi gurus, Another question, what's the deal with this microinjection whole-mount immunolabelling stuff? I read in a textbook that by injecting the Ab directly into your embryos, you increase penetration and it works even if the Ab never labelled during conventional whole mount immunohistochemistry. How time-consuming, tricky is it? What are some important things I have to consider if i wish to try this out just in case my conventional whole-mount ihc doesn't work? I'd appreciate any help from people who've done this before! thanks _________________________________________________________________ Get MSN Messenger emoticons and display pictures here! http://ilovemessenger.msn.com/?mkt=en-sg From jessgrocki <@t> yahoo.com Tue Jan 9 08:07:34 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Jan 9 08:07:43 2007 Subject: [Histonet] B-Plus Fixative Message-ID: <701057.12724.qm@web82004.mail.mud.yahoo.com> Hi, We are trying to get our pathologists to stop using mercury based fixatives for lymph node fixation. We were wondering what people's opinions are regarding non-mercury containing fixatives for lymph node fixation, particularly what you thought of B-plus fixative. Any information anyone could provide regarding any kind of lymph node fixative would be great! Thank you!! Jessica Piche-Grocki, HT (ASCP) From jessgrocki <@t> yahoo.com Tue Jan 9 08:07:34 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Jan 9 08:07:51 2007 Subject: [Histonet] B-Plus Fixative Message-ID: <701057.12724.qm@web82004.mail.mud.yahoo.com> Hi, We are trying to get our pathologists to stop using mercury based fixatives for lymph node fixation. We were wondering what people's opinions are regarding non-mercury containing fixatives for lymph node fixation, particularly what you thought of B-plus fixative. Any information anyone could provide regarding any kind of lymph node fixative would be great! Thank you!! Jessica Piche-Grocki, HT (ASCP) From Rcartun <@t> harthosp.org Tue Jan 9 08:31:04 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 9 08:31:35 2007 Subject: [Histonet] B-Plus Fixative In-Reply-To: <701057.12724.qm@web82004.mail.mud.yahoo.com> References: <701057.12724.qm@web82004.mail.mud.yahoo.com> Message-ID: <45A360D80200007700003AAC@hcnwgwds01.hh.chs> You are absolutely correct. No one should be using mercury-containing fixatives today. Several years ago, we went back to formalin for all lymphoid tissues and bone marrow biopsies. We have not had any problems as long as the specimen is fixed properly. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jessica Piche 01/09/07 9:07 AM >>> Hi, We are trying to get our pathologists to stop using mercury based fixatives for lymph node fixation. We were wondering what people's opinions are regarding non-mercury containing fixatives for lymph node fixation, particularly what you thought of B-plus fixative. Any information anyone could provide regarding any kind of lymph node fixative would be great! Thank you!! Jessica Piche-Grocki, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Rcartun <@t> harthosp.org Tue Jan 9 08:31:04 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 9 08:31:36 2007 Subject: [Histonet] B-Plus Fixative In-Reply-To: <701057.12724.qm@web82004.mail.mud.yahoo.com> References: <701057.12724.qm@web82004.mail.mud.yahoo.com> Message-ID: <45A360D80200007700003AAC@hcnwgwds01.hh.chs> You are absolutely correct. No one should be using mercury-containing fixatives today. Several years ago, we went back to formalin for all lymphoid tissues and bone marrow biopsies. We have not had any problems as long as the specimen is fixed properly. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jessica Piche 01/09/07 9:07 AM >>> Hi, We are trying to get our pathologists to stop using mercury based fixatives for lymph node fixation. We were wondering what people's opinions are regarding non-mercury containing fixatives for lymph node fixation, particularly what you thought of B-plus fixative. Any information anyone could provide regarding any kind of lymph node fixative would be great! Thank you!! Jessica Piche-Grocki, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue Jan 9 08:58:04 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue Jan 9 09:03:07 2007 Subject: [Histonet] RE: CD 10 protocol Message-ID: Hi We have a Ventana Benchmark XT and use Vector's CD 10 (clone 56C6) at 1/50 with the protocol standard CC1 primary incubation for 56 minutes at 37C and no amplification. Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From akemiat3377 <@t> yahoo.com Tue Jan 9 09:07:19 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Jan 9 09:07:28 2007 Subject: [Histonet] RUO or ASR Message-ID: <134014.25099.qm@web31301.mail.mud.yahoo.com> Hi Tim, You made my day! Thanks for the information. You gave me exactly what I wanted. I have been focusing on development of other products & setting-up TMA labs in the past few years. I have not had as intimate of a relationship with IHC as I did several years ago. Marcia Welch wanted me to propose the question to the histonet since she is not a subscriber. Best Regards, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology & TMA Madison, WI E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 From algranth <@t> u.arizona.edu Tue Jan 9 09:22:07 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jan 9 09:22:18 2007 Subject: [Histonet] Hooray for Histotechs! (have to brag just a little) Message-ID: <4.3.2.7.2.20070109080945.00da0d58@algranth.inbox.email.arizona.edu> I just had to pass this one along - we are so excited! My student, Kristin Sweetser, has just found out that she was awarded one of the ASCP student scholarships. Kristin is a student in our histology program at Pima Community College and is just about to complete her co-op in my lab. She is an exemplary student and already a very good histotech. We are excited for her and also for the program at PCC which is just a few years old. (If you want to send congrats to Kristin you can send them to this address and I will forward them to her.) Thanks for letting me brag just a little. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From rjbuesa <@t> yahoo.com Tue Jan 9 09:26:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 9 09:26:22 2007 Subject: [Histonet] Special stain on fixed cytology smear In-Reply-To: <20070108211812.11717.qmail@web50602.mail.yahoo.com> Message-ID: <20070109152605.55010.qmail@web61219.mail.yahoo.com> Yes, they can! Usually the GMS is used to detect the Pneumocystis and even IHC procedures can be done in alcohol fixed smears, and in this case they do not require HIER. Ren? J. eileen dusek wrote: Hi Everyone, Happiest of New Year A quick cytology question. Can Pneumocysytis and GMS be stained on an alcohol fixed smear? I appreciate your help Eileen Dusek __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue Jan 9 09:31:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 9 09:32:03 2007 Subject: [Histonet] B-Plus Fixative In-Reply-To: <701057.12724.qm@web82004.mail.mud.yahoo.com> Message-ID: <737016.79654.qm@web61214.mail.yahoo.com> Jessica: Mercury containing fixatives are a thing of the past; they are personally and environmentally dangerous and, although nuclear detail is very good, you can obtain similar details following very simple steps: 1-make sure your fixative is REALLY neutral, the fresher, the better; 2-fix the biopsies correctly; 3-section as thin as possible; 4-make sure that the sections are TOTALLY drained before heat drying them; and 5-use the regressive hematoxylin with acetic acid. Ren? J. Jessica Piche wrote: Hi, We are trying to get our pathologists to stop using mercury based fixatives for lymph node fixation. We were wondering what people's opinions are regarding non-mercury containing fixatives for lymph node fixation, particularly what you thought of B-plus fixative. Any information anyone could provide regarding any kind of lymph node fixative would be great! Thank you!! Jessica Piche-Grocki, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue Jan 9 09:31:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 9 09:32:11 2007 Subject: [Histonet] B-Plus Fixative In-Reply-To: <701057.12724.qm@web82004.mail.mud.yahoo.com> Message-ID: <737016.79654.qm@web61214.mail.yahoo.com> Jessica: Mercury containing fixatives are a thing of the past; they are personally and environmentally dangerous and, although nuclear detail is very good, you can obtain similar details following very simple steps: 1-make sure your fixative is REALLY neutral, the fresher, the better; 2-fix the biopsies correctly; 3-section as thin as possible; 4-make sure that the sections are TOTALLY drained before heat drying them; and 5-use the regressive hematoxylin with acetic acid. Ren? J. Jessica Piche wrote: Hi, We are trying to get our pathologists to stop using mercury based fixatives for lymph node fixation. We were wondering what people's opinions are regarding non-mercury containing fixatives for lymph node fixation, particularly what you thought of B-plus fixative. Any information anyone could provide regarding any kind of lymph node fixative would be great! Thank you!! Jessica Piche-Grocki, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bakevictoria <@t> gmail.com Tue Jan 9 09:57:45 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Jan 9 09:57:55 2007 Subject: [Histonet] Block/slide sign out in Histology Message-ID: <4f016b690701090757x241da77bm4483489e2f76d60@mail.gmail.com> Good morning all! It's been a while since I've done clinical Histology so, please bear with me if I ask questions that I really SHOULD know! In the last clinical lab I worked in we had a sign out book that was used by everyone in the lab if they removed a block, a slide or a specimen from the "physical" histology space. It was to be compliant with a certain CAP regulation. Currently I am a supervisor in a Histology lab that didn't keep a sign out book and with my taking the position I implemented one. It is meeting with strong resistance. I did check the New CAP checklist and it only refers to signing out of Histological materials from the lab for legal purposes. Can any of you share with me what your policy is for this in your lab? Thanks in advance. Vikki Baker PS - A thank you to all the people who responded to me about my stray cats I very much appreciated the assistance and support. From soofias2 <@t> yahoo.com Tue Jan 9 10:04:17 2007 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Tue Jan 9 10:04:26 2007 Subject: [Histonet] What about RUO antibodies In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F25@sjhaexc02.sjha.org> Message-ID: <903509.2322.qm@web39515.mail.mud.yahoo.com> Is it appropriate to use the RUO antibodies for diagnostic purpose in a CLIA certified lab? Any feed back greatly appreciated. Thank you in advance. Soofia "Weems, Joyce" wrote: Thanks Tim! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim Sent: Monday, January 08, 2007 6:22 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RUO OR ASR Joyce, Just to clarify, when you say the ASR is " not approved by FDA" you make it seem they cannot be used by a diagnostic lab. But the ASR designation was specifically implemented to allow diagnostic labs to use antibodies that had not been fully validated by the company that sells it and puts the onus of validation on the lab that uses it. The company must be FDA-registered to sell ASR's and only CLIA-approved diagnostic labs can use ASR's as diagnostic devices. The ASR does require a notice that it is not FDA approved, but the lab can also attach a notice that ASR's do not require FDA approval. Tim Morken Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, January 08, 2007 3:09 PM To: Akemi Allison-Tacha; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RUO OR ASR Analyte Specific Reagent - not approve by FDA... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi Allison-Tacha Sent: Monday, January 08, 2007 6:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RUO OR ASR Hi All, I hate to show my stupidity, but I must be a little out of the loop on these. I know that RUO means research use only, but what does ASR mean? Don't shoot me down in flames guys! Sincerely, Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology & TMA Madison, WI E-Mail: akemiat3377@yahoo.com Cell: (925) 788-0900 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue Jan 9 10:19:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 9 10:19:08 2007 Subject: [Histonet] Block/slide sign out in Histology In-Reply-To: <4f016b690701090757x241da77bm4483489e2f76d60@mail.gmail.com> Message-ID: <700167.2042.qm@web61216.mail.yahoo.com> Usually compliance procedures are met with resistance, if not open confrontaiton; as anything aimed at accountability and order usually does. This book to log blocks removed from their "resting place" is of paramount importance in the chain of custody and should be part, not only of your SOP procedures, but of the legal regulations of your hospital. Having all blocks and slides available and accounted for (even the parts not used of any specimen during the mandatory custody period, variable by States) is important. You can disregard the oposition and implement your policy. Never mind CAP, think in the legal consequences for your institution. CAP will not excuse you of your legal responsibilities. Ren? J. Victoria Baker wrote: Good morning all! It's been a while since I've done clinical Histology so, please bear with me if I ask questions that I really SHOULD know! In the last clinical lab I worked in we had a sign out book that was used by everyone in the lab if they removed a block, a slide or a specimen from the "physical" histology space. It was to be compliant with a certain CAP regulation. Currently I am a supervisor in a Histology lab that didn't keep a sign out book and with my taking the position I implemented one. It is meeting with strong resistance. I did check the New CAP checklist and it only refers to signing out of Histological materials from the lab for legal purposes. Can any of you share with me what your policy is for this in your lab? Thanks in advance. Vikki Baker PS - A thank you to all the people who responded to me about my stray cats I very much appreciated the assistance and support. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pmarcum <@t> vet.upenn.edu Tue Jan 9 10:23:59 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Jan 9 10:24:08 2007 Subject: [Histonet] Block/slide sign out in Histology In-Reply-To: <4f016b690701090757x241da77bm4483489e2f76d60@mail.gmail.com > References: <4f016b690701090757x241da77bm4483489e2f76d60@mail.gmail.com> Message-ID: <6.2.5.6.2.20070109111851.01c42960@vet.upenn.edu> At 10:57 AM 1/9/2007, Victoria Baker wrote: >Good morning all! > >It's been a while since I've done clinical Histology so, please bear >with me if I ask questions that I really SHOULD know! In the last >clinical lab I worked in we had a sign out book that was used by >everyone in the lab if they removed a block, a slide or a specimen >from the "physical" histology space. It was to be compliant with a >certain CAP regulation. > >Currently I am a supervisor in a Histology lab that didn't keep a sign >out book and with my taking the position I implemented one. It is >meeting with strong resistance. I did check the New CAP checklist and >it only refers to signing out of Histological materials from the lab >for legal purposes. > >Can any of you share with me what your policy is for this in your lab? > >Thanks in advance. > >Vikki Baker > > >PS - A thank you to all the people who responded to me about my stray >cats I very much appreciated the assistance and support. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi, I don't know if it is a hard rule however, we have sign out sheets for any materials, slides or blocks leaving this laboratory. We are GLP so we need this in place however, even if we were not I would have it to be sure I know where the slides and blocks are and who took them. Someone will always ask for the one that went out the door. When you don't know who took it or where it is you will be the one catching you know what for letting it disappear. I would keep pushing if possible and ask if they have ever lost a slide by letting it go to another hospital or area and needed it back only to find no one knew where or when. In medical legal cases missing an important slide is really bad news and it happens. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From GDawson <@t> dynacaremilwaukee.com Tue Jan 9 11:11:14 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jan 9 11:26:01 2007 Subject: [Histonet] Block/slide sign out in Histology Message-ID: Vikki, We have people sign out blocks/slides in a log, force them to put cardboard labels where the slides/blocks were pulled from the file (for quick reference), and they are signed out within our computer system. CAP regulations aside...this is a MUST for your own sanity. Good control over materials being taken out of the histo lab can save you from hours of fruitless searching, blame being placed on YOU for losing it & possible litigation if the patient or his/her lawyer shows up demanding these materials. Since you are the manager, I would strongly suggest you disregard the "strong resistance" you are meeting and track these things very closely for the benefit of both yourself & your employees. Those who resist the strongest are surely the ones that will pull these materials and then swear up and down that it wasn't them. Best of Luck, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, January 09, 2007 10:24 AM To: Victoria Baker; Histo Net list server Subject: Re: [Histonet] Block/slide sign out in Histology At 10:57 AM 1/9/2007, Victoria Baker wrote: >Good morning all! > >It's been a while since I've done clinical Histology so, please bear >with me if I ask questions that I really SHOULD know! In the last >clinical lab I worked in we had a sign out book that was used by >everyone in the lab if they removed a block, a slide or a specimen >from the "physical" histology space. It was to be compliant with a >certain CAP regulation. > >Currently I am a supervisor in a Histology lab that didn't keep a sign >out book and with my taking the position I implemented one. It is >meeting with strong resistance. I did check the New CAP checklist and >it only refers to signing out of Histological materials from the lab >for legal purposes. > >Can any of you share with me what your policy is for this in your lab? > >Thanks in advance. > >Vikki Baker > > >PS - A thank you to all the people who responded to me about my stray >cats I very much appreciated the assistance and support. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi, I don't know if it is a hard rule however, we have sign out sheets for any materials, slides or blocks leaving this laboratory. We are GLP so we need this in place however, even if we were not I would have it to be sure I know where the slides and blocks are and who took them. Someone will always ask for the one that went out the door. When you don't know who took it or where it is you will be the one catching you know what for letting it disappear. I would keep pushing if possible and ask if they have ever lost a slide by letting it go to another hospital or area and needed it back only to find no one knew where or when. In medical legal cases missing an important slide is really bad news and it happens. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Tue Jan 9 11:33:45 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Tue Jan 9 11:29:00 2007 Subject: [Histonet] Block/slide sign out in Histology In-Reply-To: <6.2.5.6.2.20070109111851.01c42960@vet.upenn.edu> Message-ID: <000301c73414$501bfbf0$3601a8c0@brownpathology.net> We keep documentation of what is sent where within the LIS on each patient record. If slides are sent to another pathologist within our group, but at a different location, for QA purposes, the lady that handles QA for the pathologists keeps that record. We also use a "marker" in the file to denote that something was pulled for consult, QA, or whatever, that should point you in what direction to begin looking. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, January 09, 2007 10:24 AM To: Victoria Baker; Histo Net list server Subject: Re: [Histonet] Block/slide sign out in Histology At 10:57 AM 1/9/2007, Victoria Baker wrote: >Good morning all! > >It's been a while since I've done clinical Histology so, please bear >with me if I ask questions that I really SHOULD know! In the last >clinical lab I worked in we had a sign out book that was used by >everyone in the lab if they removed a block, a slide or a specimen from >the "physical" histology space. It was to be compliant with a certain >CAP regulation. > >Currently I am a supervisor in a Histology lab that didn't keep a sign >out book and with my taking the position I implemented one. It is >meeting with strong resistance. I did check the New CAP checklist and >it only refers to signing out of Histological materials from the lab >for legal purposes. > >Can any of you share with me what your policy is for this in your lab? > >Thanks in advance. > >Vikki Baker > > >PS - A thank you to all the people who responded to me about my stray >cats I very much appreciated the assistance and support. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi, I don't know if it is a hard rule however, we have sign out sheets for any materials, slides or blocks leaving this laboratory. We are GLP so we need this in place however, even if we were not I would have it to be sure I know where the slides and blocks are and who took them. Someone will always ask for the one that went out the door. When you don't know who took it or where it is you will be the one catching you know what for letting it disappear. I would keep pushing if possible and ask if they have ever lost a slide by letting it go to another hospital or area and needed it back only to find no one knew where or when. In medical legal cases missing an important slide is really bad news and it happens. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Tue Jan 9 11:31:22 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Tue Jan 9 11:31:37 2007 Subject: [Histonet] sheep CC10 In-Reply-To: <200701091538.l09FcfNb015837@mail-proofpoint-2a.usg.tufts.edu> References: <200701091538.l09FcfNb015837@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <20070109123122.dc81ea5uskgc8k88@webmail.tufts.edu> Does anyone know of an antibody to CC10 that can be used on sheep tissue? Thanks - Melissa Mazan Quoting histonet-request@lists.utsouthwestern.edu: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: memorandum markers (Rene J Buesa) > 2. Parathyroid Storage (Weems, Joyce) > 3. RE: memorandum markers (Greer, Patricia (CDC/CCID/NCZVED)) > 4. RE: memorandum markers (Horn, Hazel V) > 5. F4/80 in FFPE mouse tissues (Horn, Diane A) > 6. Special stain on fixed cytology smear (eileen dusek) > 7. RE: Special stain on fixed cytology smear (Tony Henwood) > 8. RE: Special stain on fixed cytology smear (Weems, Joyce) > 9. RE: Special stain on fixed cytology smear (Douglas D Deltour) > 10. Sheep CD31 (AGrobe2555@aol.com) > 11. RUO OR ASR (Akemi Allison-Tacha) > 12. RE: RUO OR ASR (Weems, Joyce) > 13. RE: RUO OR ASR (Morken, Tim) > 14. RE: RUO OR ASR (Weems, Joyce) > 15. Re: Sheep CD31 (Gayle Callis) > 16. need protocol for Neu N IHC stain (Maria Mejia) > 17. Processing with Clear-Rite 3 (LeVier, Rebecca J) > 18. Microinjection whole-mount immunolabelling (gloria @home) > 19. B-Plus Fixative (Jessica Piche) > 20. B-Plus Fixative (Jessica Piche) > 21. Re: B-Plus Fixative (Richard Cartun) > 22. Re: B-Plus Fixative (Richard Cartun) > 23. RE: CD 10 protocol (Malam Jacqueline) > 24. RUO or ASR (Akemi Allison-Tacha) > 25. Hooray for Histotechs! (have to brag just a little) > (Andrea Grantham) > 26. Re: Special stain on fixed cytology smear (Rene J Buesa) > 27. Re: B-Plus Fixative (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 8 Jan 2007 10:22:09 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] memorandum markers > To: srishan@mail.holyname.org, Histonet@lists.utsouthwestern.edu > Message-ID: <20070108182209.2894.qmail@web61218.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I used to prepare them myself using 3x5 reference cards, cut to measure. > A "productive" way of using some spare time (or by son auxiliary personnel). > Ren? J. > > srishan@mail.holyname.org wrote: > > Hi, > > Happy New Year to all. > > I am looking to order memorandum markers, the dividers used in the slide > file cabinets. Any one knows where I could order it? > > Thanks in advance > > Nirmala Srishan > Histology Supervisor > Holy Name Hospital > Teaneck, NJ 07666 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 2 > Date: Mon, 8 Jan 2007 14:17:17 -0500 > From: "Weems, Joyce" > Subject: [Histonet] Parathyroid Storage > To: "Histonet" > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F0E@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > I am in search of a facility that will store parathyroids. Our > neighbor hospital has known of two locations but they are closing and > will not accept the specimen that is scheduled to be removed in > tomorrow's surgery. Any help (STAT!) would be much appreciated. > > Thanks, > Joyce > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 3 > Date: Mon, 8 Jan 2007 14:52:31 -0500 > From: "Greer, Patricia \(CDC/CCID/NCZVED\)" > Subject: RE: [Histonet] memorandum markers > To: "Rene J Buesa" , , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > We go to a local print shop and ask them to cut card stock at 3 1/4 X > 1 inch (the slide drawers had aren't very deep - you may want 3 1/2 > inch length). We get about 4000 at a time and they are much cheaper > that ordering them from some of the lab supplies. > > Pat Greer > Infectious Disease Pathology Branch > Centers for Disease Control and Prevention > Mail Stop G-32 > Atlanta, GA 30333 > > 404-639-2811 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene > J Buesa > Sent: Monday, January 08, 2007 1:22 PM > To: srishan@mail.holyname.org; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] memorandum markers > > I used to prepare them myself using 3x5 reference cards, cut to measure. > A "productive" way of using some spare time (or by son auxiliary personnel). > Ren? J. > > srishan@mail.holyname.org wrote: > > Hi, > > Happy New Year to all. > > I am looking to order memorandum markers, the dividers used in the > slide file cabinets. Any one knows where I could order it? > > Thanks in advance > > Nirmala Srishan > Histology Supervisor > Holy Name Hospital > Teaneck, NJ 07666 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Mon, 8 Jan 2007 14:35:54 -0600 > From: "Horn, Hazel V" > Subject: RE: [Histonet] memorandum markers > To: srishan@mail.holyname.org, Histonet@lists.utsouthwestern.edu > Message-ID: > <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F56@EMAIL.archildrens.org> > Content-Type: text/plain; charset=us-ascii > > You can order them from LabStorage Systems. 1.800.345.4167 > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > srishan@mail.holyname.org > Sent: Monday, January 08, 2007 11:35 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] memorandum markers > > > Hi, > > Happy New Year to all. > > I am looking to order memorandum markers, the dividers used in the slide > file cabinets. Any one knows where I could order it? > > Thanks in advance > > Nirmala Srishan > Histology Supervisor > Holy Name Hospital > Teaneck, NJ 07666 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------------ > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, > you are hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. Thank you. > ============================================================================== > > > > > ------------------------------ > > Message: 5 > Date: Mon, 8 Jan 2007 14:41:58 -0600 > From: "Horn, Diane A" > Subject: [Histonet] F4/80 in FFPE mouse tissues > To: > Message-ID: > <819189266B78D543A02D9D3956EE4F6C0484AC2D@addax.win.uthscsa.edu> > Content-Type: text/plain; charset="US-ASCII" > > I am searching for some advice on IHC with F4/80 on mouse tissues. The > antibody is from Serotec diluted 1:50 in 2% BSA/PBS. The kidney tissues > have been fixed in 4% paraformaldehyde and the liver has been fixed in > 10% NFB. The liver stains fine but the kidney has high background and > no positive staining for F4/80. I am using a kit from Biocare > (rat-on-mouse) that is a biotin free system. The only other thing is > the kidney sections were cut about 1-2 months ago and stored in a slide > box in the -20 freezer (not frost free). Any advice or protocol would > be appreciated! > > > > Thanks to all, > > Diane > > > > Diane A. Horn > > Department of Pathology > > UTHSCSA > > 7703 Floyd Curl Dr. > > San Antonio, TX 78229 > > 210-567-4039 > > > > > > ------------------------------ > > Message: 6 > Date: Mon, 8 Jan 2007 13:18:12 -0800 (PST) > From: eileen dusek > Subject: [Histonet] Special stain on fixed cytology smear > To: histonet > Cc: me > Message-ID: <20070108211812.11717.qmail@web50602.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Everyone, > Happiest of New Year > > A quick cytology question. > Can Pneumocysytis and GMS be stained on an alcohol fixed smear? > > I appreciate your help > > Eileen Dusek > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 7 > Date: Tue, 9 Jan 2007 08:32:42 +1100 > From: "Tony Henwood" > Subject: RE: [Histonet] Special stain on fixed cytology smear > To: "eileen dusek" , "histonet" > > Cc: me > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Yep, > Sure can > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen > dusek > Sent: Tuesday, 9 January 2007 8:18 AM > To: histonet > Cc: me > Subject: [Histonet] Special stain on fixed cytology smear > > > Hi Everyone, > Happiest of New Year > > A quick cytology question. > Can Pneumocysytis and GMS be stained on an alcohol fixed smear? > > I appreciate your help > > Eileen Dusek > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please delete > it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The > Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, the > Childrens Hospital at Westmead accepts no liability for any > consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 8 > Date: Mon, 8 Jan 2007 16:36:06 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Special stain on fixed cytology smear > To: "eileen dusek" , "histonet" > > Cc: me > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F1C@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Yes - they work fine. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of eileen > dusek > Sent: Monday, January 08, 2007 4:18 PM > To: histonet > Cc: me > Subject: [Histonet] Special stain on fixed cytology smear > > > Hi Everyone, > Happiest of New Year > > A quick cytology question. > Can Pneumocysytis and GMS be stained on an alcohol fixed smear? > > I appreciate your help > > Eileen Dusek > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 9 > Date: Mon, 8 Jan 2007 16:39:57 -0500 > From: "Douglas D Deltour" > Subject: RE: [Histonet] Special stain on fixed cytology smear > To: "'eileen dusek'" , "'histonet'" > > Cc: 'me' > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Yes it can. > > > Douglas D. Deltour HT(ASCP) > Histology Supervisor > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen dusek > Sent: Monday, January 08, 2007 4:18 PM > To: histonet > Cc: me > Subject: [Histonet] Special stain on fixed cytology smear > > Hi Everyone, > Happiest of New Year > > A quick cytology question. > Can Pneumocysytis and GMS be stained on an alcohol fixed smear? > > I appreciate your help > > Eileen Dusek > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > Message: 10 > Date: Mon, 8 Jan 2007 17:51:51 EST > From: AGrobe2555@aol.com > Subject: [Histonet] Sheep CD31 > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Does anyone know of an anti-CD31 that works on sheep FFPE tissues? > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > ------------------------------ > > Message: 11 > Date: Mon, 8 Jan 2007 15:02:33 -0800 (PST) > From: Akemi Allison-Tacha > Subject: [Histonet] RUO OR ASR > To: Histonet@lists.utsouthwestern.edu > Message-ID: <255459.23548.qm@web31304.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi All, > > I hate to show my stupidity, but I must be a little out of the loop > on these. I know that RUO means research use only, but what does ASR > mean? Don't shoot me down in flames guys! > > Sincerely, > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology & TMA > Madison, WI > E-Mail: akemiat3377@yahoo.com > Cell: (925) 788-0900 > > > > > ------------------------------ > > Message: 12 > Date: Mon, 8 Jan 2007 18:08:39 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] RUO OR ASR > To: "Akemi Allison-Tacha" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F24@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Analyte Specific Reagent - not approve by FDA... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi > Allison-Tacha > Sent: Monday, January 08, 2007 6:03 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] RUO OR ASR > > > Hi All, > > I hate to show my stupidity, but I must be a little out of the loop > on these. I know that RUO means research use only, but what does ASR > mean? Don't shoot me down in flames guys! > > Sincerely, > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology & TMA > Madison, WI > E-Mail: akemiat3377@yahoo.com > Cell: (925) 788-0900 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 13 > Date: Mon, 8 Jan 2007 18:22:14 -0500 > From: "Morken, Tim" > Subject: RE: [Histonet] RUO OR ASR > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Joyce, Just to clarify, when you say the ASR is " not approved by FDA" > you make it seem they cannot be used by a diagnostic lab. But the ASR > designation was specifically implemented to allow diagnostic labs to use > antibodies that had not been fully validated by the company that sells > it and puts the onus of validation on the lab that uses it. The company > must be FDA-registered to sell ASR's and only CLIA-approved diagnostic > labs can use ASR's as diagnostic devices. The ASR does require a notice > that it is not FDA approved, but the lab can also attach a notice that > ASR's do not require FDA approval. > > > Tim Morken > Lab Vision - Neomarkers > ThermoFisher Scientific > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Monday, January 08, 2007 3:09 PM > To: Akemi Allison-Tacha; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RUO OR ASR > > Analyte Specific Reagent - not approve by FDA... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi > Allison-Tacha > Sent: Monday, January 08, 2007 6:03 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] RUO OR ASR > > > Hi All, > > I hate to show my stupidity, but I must be a little out of the loop on > these. I know that RUO means research use only, but what does ASR mean? > Don't shoot me down in flames guys! > > Sincerely, > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology & TMA > Madison, WI > E-Mail: akemiat3377@yahoo.com > Cell: (925) 788-0900 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. Thank you. > Saint Joseph's Health System, Inc. > > > > ------------------------------ > > Message: 14 > Date: Mon, 8 Jan 2007 18:32:44 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] RUO OR ASR > To: "Morken, Tim" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F25@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Thanks Tim! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, > Tim > Sent: Monday, January 08, 2007 6:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RUO OR ASR > > > Joyce, Just to clarify, when you say the ASR is " not approved by FDA" > you make it seem they cannot be used by a diagnostic lab. But the ASR > designation was specifically implemented to allow diagnostic labs to use > antibodies that had not been fully validated by the company that sells > it and puts the onus of validation on the lab that uses it. The company > must be FDA-registered to sell ASR's and only CLIA-approved diagnostic > labs can use ASR's as diagnostic devices. The ASR does require a notice > that it is not FDA approved, but the lab can also attach a notice that > ASR's do not require FDA approval. > > > Tim Morken > Lab Vision - Neomarkers > ThermoFisher Scientific > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Monday, January 08, 2007 3:09 PM > To: Akemi Allison-Tacha; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RUO OR ASR > > Analyte Specific Reagent - not approve by FDA... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi > Allison-Tacha > Sent: Monday, January 08, 2007 6:03 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] RUO OR ASR > > > Hi All, > > I hate to show my stupidity, but I must be a little out of the loop on > these. I know that RUO means research use only, but what does ASR mean? > Don't shoot me down in flames guys! > > Sincerely, > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology & TMA > Madison, WI > E-Mail: akemiat3377@yahoo.com > Cell: (925) 788-0900 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. Thank you. > Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 15 > Date: Mon, 08 Jan 2007 16:40:21 -0700 > From: Gayle Callis > Subject: Re: [Histonet] Sheep CD31 > To: AGrobe2555@aol.com, Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070108163815.01b3cd18@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Serotec veterinary antibody chart indicates CO-31D4 should work, check with > their technical services to make sure this is true. This is their number, > Cat # or clone ???? > > t 03:51 PM 1/8/2007, you wrote: >> Does anyone know of an anti-CD31 that works on sheep FFPE tissues? >> Thanks, >> Albert >> >> Albert C. Grobe, PhD >> International Heart Institute of Montana Foundation >> Tissue Engineering Lab, Saint Patrick Hospital > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > > > ------------------------------ > > Message: 16 > Date: Mon, 8 Jan 2007 22:37:21 -0800 > From: Maria Mejia > Subject: [Histonet] need protocol for Neu N IHC stain > To: histonet@lists.utsouthwestern.edu > Message-ID: <085A3F3A-F870-4C92-93ED-DA584B575EB8@gmail.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hello, > > Please, could someone provide a Neu N (neuron specific marker) IFA > IHC protocol? > I need to stain 40um cryostat free-floating primate fixed sections > using this marker. I would > welcome any suggestions from vendors too. > > I look forward to hearing from you. > > Maria Bartola Mejia > UCSF > Department of Neurosurgery > San Francisco, CA > > > > ------------------------------ > > Message: 17 > Date: Tue, 9 Jan 2007 08:08:24 -0500 > From: "LeVier, Rebecca J" > Subject: [Histonet] Processing with Clear-Rite 3 > To: > Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2019E37C7@MAIL-LR.lha.org> > Content-Type: text/plain; charset="us-ascii" > > Please help.....If you are using Clear-Rite 3 and also processing on a > shorter biopsy schedule, would you please share the times you are using > at each station? > > Thanks in advance for all your help! > > Rebecca J. LeVier HT(ASCP) > Histology Supervisor > > Email: rjlevier@lancastergeneral.org > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > ------------------------------ > > Message: 18 > Date: Tue, 09 Jan 2007 13:35:01 +0000 > From: "gloria @home" > Subject: [Histonet] Microinjection whole-mount immunolabelling > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > Hi gurus, > > Another question, what's the deal with this microinjection whole-mount > immunolabelling stuff? I read in a textbook that by injecting the Ab > directly into your embryos, you increase penetration and it works even if > the Ab never labelled during conventional whole mount immunohistochemistry. > > How time-consuming, tricky is it? > > What are some important things I have to consider if i wish to try this out > just in case my conventional whole-mount ihc doesn't work? > > I'd appreciate any help from people who've done this before! > > thanks > > _________________________________________________________________ > Get MSN Messenger emoticons and display pictures here! > http://ilovemessenger.msn.com/?mkt=en-sg > > > > > ------------------------------ > > Message: 19 > Date: Tue, 9 Jan 2007 06:07:34 -0800 (PST) > From: Jessica Piche > Subject: [Histonet] B-Plus Fixative > To: histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu > Message-ID: <701057.12724.qm@web82004.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi, > > We are trying to get our pathologists to stop using mercury based > fixatives for lymph node fixation. We were wondering what people's > opinions are regarding non-mercury containing fixatives for lymph > node fixation, particularly what you thought of B-plus fixative. > > Any information anyone could provide regarding any kind of lymph > node fixative would be great! > > Thank you!! > > Jessica Piche-Grocki, HT (ASCP) > > > ------------------------------ > > Message: 20 > Date: Tue, 9 Jan 2007 06:07:34 -0800 (PST) > From: Jessica Piche > Subject: [Histonet] B-Plus Fixative > To: histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu > Message-ID: <701057.12724.qm@web82004.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi, > > We are trying to get our pathologists to stop using mercury based > fixatives for lymph node fixation. We were wondering what people's > opinions are regarding non-mercury containing fixatives for lymph > node fixation, particularly what you thought of B-plus fixative. > > Any information anyone could provide regarding any kind of lymph > node fixative would be great! > > Thank you!! > > Jessica Piche-Grocki, HT (ASCP) > > > ------------------------------ > > Message: 21 > Date: Tue, 09 Jan 2007 09:31:04 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] B-Plus Fixative > To: , > , "Jessica Piche" > Message-ID: <45A360D80200007700003AAC@hcnwgwds01.hh.chs> > Content-Type: text/plain; charset=US-ASCII > > You are absolutely correct. No one should be using mercury-containing > fixatives today. Several years ago, we went back to formalin for all > lymphoid tissues and bone marrow biopsies. We have not had any problems > as long as the specimen is fixed properly. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> Jessica Piche 01/09/07 9:07 AM >>> > Hi, > > We are trying to get our pathologists to stop using mercury based > fixatives for lymph node fixation. We were wondering what people's > opinions are regarding non-mercury containing fixatives for lymph node > fixation, particularly what you thought of B-plus fixative. > > Any information anyone could provide regarding any kind of lymph node > fixative would be great! > > Thank you!! > > Jessica Piche-Grocki, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and > destroy all copies of the original message. > > > > ------------------------------ > > Message: 22 > Date: Tue, 09 Jan 2007 09:31:04 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] B-Plus Fixative > To: , > , "Jessica Piche" > Message-ID: <45A360D80200007700003AAC@hcnwgwds01.hh.chs> > Content-Type: text/plain; charset=US-ASCII > > You are absolutely correct. No one should be using mercury-containing > fixatives today. Several years ago, we went back to formalin for all > lymphoid tissues and bone marrow biopsies. We have not had any problems > as long as the specimen is fixed properly. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> Jessica Piche 01/09/07 9:07 AM >>> > Hi, > > We are trying to get our pathologists to stop using mercury based > fixatives for lymph node fixation. We were wondering what people's > opinions are regarding non-mercury containing fixatives for lymph node > fixation, particularly what you thought of B-plus fixative. > > Any information anyone could provide regarding any kind of lymph node > fixative would be great! > > Thank you!! > > Jessica Piche-Grocki, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and > destroy all copies of the original message. > > > > ------------------------------ > > Message: 23 > Date: Tue, 9 Jan 2007 14:58:04 -0000 > From: Malam Jacqueline > Subject: [Histonet] RE: CD 10 protocol > To: "Histonet submissions (histonet@lists.utsouthwestern.edu)" > > Message-ID: > Content-Type: text/plain > > Hi > We have a Ventana Benchmark XT and use Vector's CD 10 (clone 56C6) at 1/50 > with the protocol standard CC1 primary incubation for 56 minutes at 37C and > no amplification. > > Jacqui > > > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > > > > ------------------------------ > > Message: 24 > Date: Tue, 9 Jan 2007 07:07:19 -0800 (PST) > From: Akemi Allison-Tacha > Subject: [Histonet] RUO or ASR > To: Histonet@lists.utsouthwestern.edu > Message-ID: <134014.25099.qm@web31301.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Tim, > > You made my day! Thanks for the information. You gave me exactly > what I wanted. I have been focusing on development of other > products & setting-up TMA labs in the past few years. I have not had > as intimate of a relationship with IHC as I did several years ago. > Marcia Welch wanted me to propose the question to the histonet since > she is not a subscriber. > > Best Regards, > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology & TMA > Madison, WI > E-Mail: akemiat3377@yahoo.com > Cell: (925) 788-0900 > > > > ------------------------------ > > Message: 25 > Date: Tue, 09 Jan 2007 08:22:07 -0700 > From: Andrea Grantham > Subject: [Histonet] Hooray for Histotechs! (have to brag just a > little) > To: histonet@lists.utsouthwestern.edu > Message-ID: > <4.3.2.7.2.20070109080945.00da0d58@algranth.inbox.email.arizona.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > I just had to pass this one along - we are so excited! > My student, Kristin Sweetser, has just found out that she was awarded one > of the ASCP student scholarships. Kristin is a student in our histology > program at Pima Community College and is just about to complete her co-op > in my lab. She is an exemplary student and already a very good histotech. > We are excited for her and also for the program at PCC which is just a few > years old. > > (If you want to send congrats to Kristin you can send them to this address > and I will forward them to her.) > > Thanks for letting me brag just a little. > > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > > > ------------------------------ > > Message: 26 > Date: Tue, 9 Jan 2007 07:26:05 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Special stain on fixed cytology smear > To: eileen dusek , histonet > > Cc: me > Message-ID: <20070109152605.55010.qmail@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Yes, they can! > Usually the GMS is used to detect the Pneumocystis and even IHC > procedures can be done in alcohol fixed smears, and in this case they > do not require HIER. > Ren? J. > > eileen dusek wrote: > Hi Everyone, > Happiest of New Year > > A quick cytology question. > Can Pneumocysytis and GMS be stained on an alcohol fixed smear? > > I appreciate your help > > Eileen Dusek > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 27 > Date: Tue, 9 Jan 2007 07:31:54 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] B-Plus Fixative > To: Jessica Piche , > histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu > Message-ID: <737016.79654.qm@web61214.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Jessica: > Mercury containing fixatives are a thing of the past; they are > personally and environmentally dangerous and, although nuclear detail > is very good, you can obtain similar details following very simple > steps: > 1-make sure your fixative is REALLY neutral, the fresher, the better; > 2-fix the biopsies correctly; > 3-section as thin as possible; > 4-make sure that the sections are TOTALLY drained before heat drying > them; and > 5-use the regressive hematoxylin with acetic acid. > Ren? J. > > Jessica Piche wrote: > Hi, > > We are trying to get our pathologists to stop using mercury based > fixatives for lymph node fixation. We were wondering what people's > opinions are regarding non-mercury containing fixatives for lymph > node fixation, particularly what you thought of B-plus fixative. > > Any information anyone could provide regarding any kind of lymph node > fixative would be great! > > Thank you!! > > Jessica Piche-Grocki, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 38, Issue 10 > **************************************** > Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu From tim.morken <@t> thermofisher.com Tue Jan 9 11:45:09 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue Jan 9 11:46:54 2007 Subject: [Histonet] Block/slide sign out in Histology In-Reply-To: Message-ID: Vikki, people are often resistant to entering data, but when they can use that data later they usually realize the value. In this case it would seem that being able to find slides easily would make life easier for everyone. It may mean pain now, but the results will be satisfying later ( Gee, Dr., you have 200 slide folders in your office, do you think that one slide you need might be in here somewhere?!?!). Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, January 09, 2007 9:11 AM To: Histo Net list server Subject: RE: [Histonet] Block/slide sign out in Histology Vikki, We have people sign out blocks/slides in a log, force them to put cardboard labels where the slides/blocks were pulled from the file (for quick reference), and they are signed out within our computer system. CAP regulations aside...this is a MUST for your own sanity. Good control over materials being taken out of the histo lab can save you from hours of fruitless searching, blame being placed on YOU for losing it & possible litigation if the patient or his/her lawyer shows up demanding these materials. Since you are the manager, I would strongly suggest you disregard the "strong resistance" you are meeting and track these things very closely for the benefit of both yourself & your employees. Those who resist the strongest are surely the ones that will pull these materials and then swear up and down that it wasn't them. Best of Luck, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, January 09, 2007 10:24 AM To: Victoria Baker; Histo Net list server Subject: Re: [Histonet] Block/slide sign out in Histology At 10:57 AM 1/9/2007, Victoria Baker wrote: >Good morning all! > >It's been a while since I've done clinical Histology so, please bear >with me if I ask questions that I really SHOULD know! In the last >clinical lab I worked in we had a sign out book that was used by >everyone in the lab if they removed a block, a slide or a specimen from >the "physical" histology space. It was to be compliant with a certain >CAP regulation. > >Currently I am a supervisor in a Histology lab that didn't keep a sign >out book and with my taking the position I implemented one. It is >meeting with strong resistance. I did check the New CAP checklist and >it only refers to signing out of Histological materials from the lab >for legal purposes. > >Can any of you share with me what your policy is for this in your lab? > >Thanks in advance. > >Vikki Baker > > >PS - A thank you to all the people who responded to me about my stray >cats I very much appreciated the assistance and support. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi, I don't know if it is a hard rule however, we have sign out sheets for any materials, slides or blocks leaving this laboratory. We are GLP so we need this in place however, even if we were not I would have it to be sure I know where the slides and blocks are and who took them. Someone will always ask for the one that went out the door. When you don't know who took it or where it is you will be the one catching you know what for letting it disappear. I would keep pushing if possible and ask if they have ever lost a slide by letting it go to another hospital or area and needed it back only to find no one knew where or when. In medical legal cases missing an important slide is really bad news and it happens. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From YuJ2 <@t> upmc.edu Tue Jan 9 12:32:06 2007 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Tue Jan 9 12:32:14 2007 Subject: [Histonet] slide and cassette storage for a research In-Reply-To: Message-ID: <7E0A77BFEB9A1E47A63F978E7116821F02AEC61A@1upmc-msx11.acct.upmchs.net> Dear histonetters, Need your help on this. I have a small research lab, looking for an economical way to organize slides and tissue cassettes. We are talking about up to a few thousand cassettes and ten thousand of slides. We are getting to the point not happy with storing cassettes in cardboard boxes and slides in slide boxes (holding 100 slides/EA) Any suggestions? Thank you very much for your help. ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Hillman Cancer Center Research Pavilion Office Suite 2.26h, Laboratory 2.43 5117 Centre Avenue, Pittsburgh, PA 15213 Phone: 412-623-7786, (Lab) 412-623-3255 Email: yuj2@upmc.edu ******************************************************************* -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Friday, January 20, 2006 9:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide storage feedback Thanks everyone for your response! I got a lot of advice on long term slide storage sent to me privately, so just to share, I would say the general consensus was ultra low temps wrapped in foil along with a desiccant. Thanks again to the wonderful Mr. Tim Morken for sharing this article on the TMA storage and the paraffin "dip". The technique was what the Pathologist was looking for and what we will be using in our project. The Histonet is such a valuable tool, and I appreciate the support and help I have gotten from peers as well as vendors. Have the best weekend possible! I'll be spending my Saturday shoveling snow. Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 21 Date: Thu, 19 Jan 2006 15:50:02 -0600 WS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Jan 9 13:20:17 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jan 9 13:20:34 2007 Subject: [Histonet] FW: Legislative Action Needed: Tell New Congress to Stop Pod Labs Message-ID: <004701c73423$332bd9c0$6501a8c0@Patsy> FYI _____ From: ASCP Washington [mailto:ascp@site1.ascpmail.org] Sent: Tuesday, January 09, 2007 12:16 PM To: Patsy G Ruegg Subject: Legislative Action Needed: Tell New Congress to Stop Pod Labs Tell CMS and Congress: Pod Labs Undermine Quality Patient Care! Take a few minutes to help stop pod labs and their abusive billing practices. ASCP is urging all members of the laboratory team to demand that the government "STOP POD LABS NOW!" Pathology and laboratory medicine was dealt an unexpected blow last November when the Centers for Medicare & Medicaid Services (CMS) declined to implement a series of initiatives designed to stop abusive billing practices by "pod" or "condo" laboratories. ASCP is asking all laboratory practitioners to demand CMS take action against these abusive billing practices. The entire laboratory team is affected by these pods as they employ both pathologists and medical technologists/technicians in their scheme. "Pod labs and fee splitting threaten the very existence of pathology and laboratory medicine. ASCP pledges to be relentless in our pursuit to stop pod labs now." - John S. J. Brooks, M.D., FASCP, ASCP President What's a pod lab and why are they an issue? Pod laboratories are facilities structured to enable referring providers to rent space (such as a cubicle) in a laboratory, to exploit a loophole in Medicare's in-office ancillary exception rules. These labs provide a limited menu of services, such as analyzing biopsies, often at below fair market value. In 2005, CMS relaxed its rules for independent contractor physicians or non-physicians to reassign their Medicare billing rights to health care entities. Previously, CMS' regulations allowed reassignment of benefits only for those services performed on-site. With pod labs, the new in-office ancillary exception rules can be abused to enable referring providers to capture revenues generated from pathology services. Why are pod labs bad? ASCP has condemned these arrangements because they adversely affect patient care, the quality of laboratory services, and the laboratory industry. By charging a fraction of what a full-scale clinical laboratory typically charges, pod labs empower referring providers to mark-up the cost of the Medicare laboratory services. These economic incentives distort rational medical decisions by enabling providers to be compensated on the volume of laboratory services referred. It also encourages overutilization of testing services, which can increase the risk of injury to the patient. The Wall Street Journal has run several articles on pod labs, including a feature on October 23, 2006 that stated "patients, in some cases, are being referred for tests.at lower-quality labs simply because the referring physician stands to get a cut of the profits from that work." These arrangements also adversely affect the quality laboratory services industry-wide, because laboratories seeking to remain competitive may increasingly be forced to focus more on cost than on quality. ASCP's policy statement on Fee Splitting, Mark-Ups and Related Practices, and the American Medical Association, through its Council on Ethical and Judicial Affairs expressed strong opposition to fee-splitting and mark-ups. One of the policy statements states that "a physician who disregards quality as the primary criterion or who chooses a laboratory solely because it provides low-cost laboratory services on which the patient is charged a profit, is not acting in the best interests of the patient." Pod labs may also be responsible, in part, for some of CMS' recent efforts to combat Medicare fraud. Some of these initiatives, such as the national correct coding initiative and the medically unlikely (formerly unbelievable) edits (MUEs) program, have adverse consequences for patient care by interfering with the ability of pathologists and other laboratory professionals to provide necessary laboratory services. It can be recalled that one of CMS' proposed MUEs would have capped reimbursement for the examination of biopsies at no more than two per patient per day, regardless of medical necessity. ASCP continues to fight these efforts. ASCP Advocacy Efforts. ASCP is stepping up its advocacy efforts to prevent these entities from undermining patient care and the quality laboratory services performed by our nation's hospital and independent reference clinical laboratories. ASCP's recent comments on the 2007 physician fee schedule urged strong measures to stop pod labs and similar abusive billing schemes. Unfortunately, pressure from groups representing those who benefit financially from these arrangements and comments submitted by a major medical association raising concern about "unintended consequences" lead the agency to back off. ASCP is launching this grassroots advocacy campaign to change CMS' mind. What You Can Do Visit ASCP 's e-Advocacy Center to send CMS and your members of Congress a brief message urging CMS to stop pod labs from engaging in abusive billing practices. ASCP's Washington staff has drafted a model email you can send but we encourage you to personalize your email by noting how these entities have adversely affected the work you do. This will significantly increase the effectiveness of your message to CMS. It only take a few minutes to help stop pod labs now!!! This alert can be accessed by clicking here. ASCP's latest coverage of the Anti-Pod Lab Campaign, as written in the latest edition of ASCP e-Policy News, can be accessed by clicking here. Please forward this email to your colleagues to increase the impact your efforts can have to help stop pod labs. Tell a friend: Not everyone receives ASCP's Action Alerts, so please forward this message to your peers and co-workers! C 2007 American Society for Clinical Pathology 33 West Monroe Street, Suite 1600, Chicago, IL 60603 312.541.4999 ~ info@ascp.org ABOUT THIS MESSAGE You are receiving this email because you are a member of ASCP, have attended ASCP programs/meetings or made a purchase from ASCP. If you no longer wish to receive emails of this kind from ASCP, please login to change your email preferences at ascp.org. This email message complies with all CAN-SPAM 2004 regulations. It was sent to you by the AMERICAN SOCIETY FOR CLINICAL PATHOLOGY, 33 West Monroe, Suite 1600, Chicago, IL 60603 From Heather.D.Renko <@t> osfhealthcare.org Tue Jan 9 14:17:14 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Jan 9 14:17:35 2007 Subject: [Histonet] Open postion in Rockford, Illinois Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612C8E@pmc-rfd-mx01.intranet.osfnet.org> We are posting a position for an ASCP registered HT, Histotechnician I in our pathology laboratory. Please apply via the website www.osfhealthcare.org or contact me for more information. This is a full time position with good benefits and generous paid time off. Located in Rockford, Illinois. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From nmarinos <@t> mail.nih.gov Tue Jan 9 14:20:36 2007 From: nmarinos <@t> mail.nih.gov (Nancy Marinos) Date: Tue Jan 9 14:20:49 2007 Subject: [Histonet] Please take me off the mailing list until April 23, 2007 Message-ID: -- I will sign on again after 4/23. Thanks, Nancy Marinos From dpapalegis <@t> gmail.com Tue Jan 9 14:27:30 2007 From: dpapalegis <@t> gmail.com (Derek Papalegis) Date: Tue Jan 9 14:27:36 2007 Subject: [Histonet] new lab Message-ID: <73382ce30701091227x4fbcc310qb455b28e0d3f0995@mail.gmail.com> I am starting a new histo lab in a small animal facility and need to buy EVERYTHING. The space we have is very limited so finding the right equipment could be tough. Does anyone have any thoughts on the table top carousel processors? i know richard allen, leica and thermo all have them. are they any good? also with stainers, does anyone have any thoughts about Thermo's Varistain Gemini? It looks compact enough to work in the limited space we have. If anyone could give me feedback about these pieces of equipment, about any new equipment in general or about anything related to starting a lab from scatch, I would greatly appreciate it. thanks, Derek From ploykasek <@t> phenopath.com Tue Jan 9 14:40:02 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jan 9 14:40:20 2007 Subject: [Histonet] Control tissue. Message-ID: Hi all. I was wondering if anyone knows of a commercial source to buy IHC positive control tissue/slides. I am primarily interested in positive controls for amyloid A and VZV. Thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From HornHV <@t> archildrens.org Tue Jan 9 14:43:18 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jan 9 14:43:46 2007 Subject: [Histonet] cassette/slide labeler Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F62@EMAIL.archildrens.org> It's capital equipment time at our hospital. I am going to ask for a cassette/slide labeler. Who makes your favorite and what do you like best/least about it? Please reply directly to me if you feel more comfortable doing so. Vendors may reply too. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From godsgalnow <@t> aol.com Tue Jan 9 14:55:33 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Jan 9 14:55:57 2007 Subject: [Histonet] new lab In-Reply-To: <73382ce30701091227x4fbcc310qb455b28e0d3f0995@mail.gmail.com> References: <73382ce30701091227x4fbcc310qb455b28e0d3f0995@mail.gmail.com> Message-ID: <8C90247A3753146-46C-A18A@FWM-D36.sysops.aol.com> Derek, I have 4 of the Gemini stainers from Thermo and we like it. It has a smaller footprint than the typical linear stainers. It is easy to use and program. And programs and be saved on a disk so if there is ever a power failure, you just pop the disk in. Roxanne -----Original Message----- From: dpapalegis@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Tue, 9 Jan 2007 3:27 PM Subject: [Histonet] new lab I am starting a new histo lab in a small animal facility and need to buy EVERYTHING. The space we have is very limited so finding the right equipment could be tough. Does anyone have any thoughts on the table top carousel processors? i know richard allen, leica and thermo all have them. are they any good? also with stainers, does anyone have any thoughts about Thermo's Varistain Gemini? It looks compact enough to work in the limited space we have. If anyone could give me feedback about these pieces of equipment, about any new equipment in general or about anything related to starting a lab from scatch, I would greatly appreciate it. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From ploykasek <@t> phenopath.com Tue Jan 9 15:18:36 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jan 9 15:18:50 2007 Subject: [Histonet] Control tissue. In-Reply-To: <6.0.0.22.1.20070109135423.01b3a1c0@gemini.msu.montana.edu> Message-ID: Sorry for the abbreviation - poor form on my part. VZV is varicella zoster virus. Thanks. Patti > What is VZV? > > At 01:40 PM 1/9/2007, you wrote: >> Hi all. I was wondering if anyone knows of a commercial source to buy IHC >> positive control tissue/slides. I am primarily interested in positive >> controls for amyloid A and VZV. Thanks for the help. >> >> >> Patti Loykasek BS, HTL, QIHC >> PhenoPath Laboratories >> Seattle, WA >> >> >> >> ------------------------------------------------------------------------- >> This e-mail message, including any attachments, is for the sole use of >> the intended recipients and may contain privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If >> you are not the intended recipient, please contact the sender by e-mail >> and destroy all copies of the original message, or you may call PhenoPath >> Laboratories, Seattle, WA U.S.A. at (206) 374-9000. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From RSRICHMOND <@t> aol.com Tue Jan 9 15:29:23 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Jan 9 15:29:39 2007 Subject: [Histonet] Re: Parathyroid Storage Message-ID: Joyce Weems asks about parathyroid storage. I suppose she means facilities for holding frozen parathyroid tissue for possible re-implantation after parathyroid surgery. I think there is such a facility in Charlotte NC, probably at Charlotte Medical Center. I'm on the road and can't check my records. Our experience in Gastonia was that we sent a lot of parathyroids to the facility, but at least as far as we knew, none was ever asked for. Bob Richmond Samurai Pathologist Knoxville TN From ploykasek <@t> phenopath.com Tue Jan 9 17:00:11 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jan 9 17:00:25 2007 Subject: [Histonet] Blood Group Antibodies Message-ID: Does anyone have a good supplier for antibodies to blood group antigens? Last year our usual supplier discontinued the antibodies. We got in new antibodies from new vendor, worked them up & validated them. Now that vendor has discontinued the antibodies. Here we go again. Thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From JWEEMS <@t> sjha.org Tue Jan 9 17:22:30 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Jan 9 17:23:03 2007 Subject: [Histonet] Charge for packaging slides for sendouts Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F48@sjhaexc02.sjha.org> It seems that it we just recently had this discussion, but I was unable to find it by searching. I don't remember what the consensus was, but if I recall correctly, there is no CPT code that will allow us to charge a handling fee for recuts, packaging, and shipping cases. One more time... what are you doing in this regard? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Tue Jan 9 18:09:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 9 18:09:34 2007 Subject: [Histonet] Charge for packaging slides for sendouts In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F48@sjhaexc02.sjha.org> Message-ID: <628416.72024.qm@web61223.mail.yahoo.com> We used to charge a fee to those requesting the service. Depending on the amount, it was negotiable. Ren? J. "Weems, Joyce" wrote: It seems that it we just recently had this discussion, but I was unable to find it by searching. I don't remember what the consensus was, but if I recall correctly, there is no CPT code that will allow us to charge a handling fee for recuts, packaging, and shipping cases. One more time... what are you doing in this regard? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Ebreisch <@t> chsd.org Tue Jan 9 18:20:45 2007 From: Ebreisch <@t> chsd.org (Breisch, Eric) Date: Tue Jan 9 18:20:55 2007 Subject: [Histonet] CAP regulations regarding storage of antibodies in frost free refrigerator freezers Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B31A5992@e2k3backend1.RCHSD.org> Hi Histonet- Is anyone aware of new or existing CAP regulations regarding the use of frost free refrigerator/freezers for the storage of antibodies ie. IFA antibodies or IHC antibodies? Please advise if anyone has information pertaining to Histology and the above mentioned use of a refrigerator/freezer. Thank you, Eric A. Breisch, Ph.D. Clinical Anatomist Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy UCSD SOM From Megan.Clarke <@t> hnehealth.nsw.gov.au Tue Jan 9 21:21:06 2007 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Tue Jan 9 21:21:47 2007 Subject: [Histonet] Fwd: suppliers of antibodies Message-ID: >>> Megan Clarke 01/10/07 11:58 AM >>> Hi Histonetters HAPPY NEW YEAR & WISHING YOU THE BEST FOR 2007! I am searching for suppliers of a few antibodies that can only be used on formalin-fixed paraffin-embedded tissues that we would like to purchase, in particular, the agents in Australia that may be able to supply them to us. If anyone out there has any information, please advise. These are the antibodies: 1.Monoclonal Anti-BAF47 (1N11) antibody- clone 25 . BD Transduction laboratories. San Diego. 2.Caveolin-3 (Cav-3) monoclonal antibody, Transduction laboratories, Lexington, KY or Accurate Chemical who supply a polyclonal antibody 3. Desmoplakin 1 &2 - Progen Biotech, Heidelberg, Germany 4. K-2 ( clone of Ki-67).????? 5.SYT protein & SSX1 (Atlas antibodies) Thank you for your assistance. Its always good to know there are very knowledgeable academics out there. Zenobia Haffajee HAPS IHC dept Newcastle Australia From immrstambo <@t> hotmail.com Wed Jan 10 07:11:11 2007 From: immrstambo <@t> hotmail.com (Chistine Tambasco) Date: Wed Jan 10 07:11:05 2007 Subject: [Histonet] Hooray for Histotechs! (have to brag just a little) References: <4.3.2.7.2.20070109080945.00da0d58@algranth.inbox.email.arizona.edu> Message-ID: Congratulations Kristin!! I know the feeling as I was a former Technicon Student Scholarship winner in 1982. (Wow am I getting old!) Best of luck in your future career! Christine Tambasco HT (ASCP) St. Mary's Hospital Amsterdam, New York ----- Original Message ----- From: "Andrea Grantham" To: Sent: Tuesday, January 09, 2007 10:22 AM Subject: [Histonet] Hooray for Histotechs! (have to brag just a little) >I just had to pass this one along - we are so excited! > My student, Kristin Sweetser, has just found out that she was awarded one > of the ASCP student scholarships. Kristin is a student in our histology > program at Pima Community College and is just about to complete her co-op > in my lab. She is an exemplary student and already a very good histotech. > We are excited for her and also for the program at PCC which is just a few > years old. > > (If you want to send congrats to Kristin you can send them to this address > and I will forward them to her.) > > Thanks for letting me brag just a little. > > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Wed Jan 10 08:10:28 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jan 10 08:10:54 2007 Subject: [Histonet] CAP regulations regarding storage of antibodies in frost free refrigerator freezers In-Reply-To: <43B97B4C402C2C44AAA2A8D2C86A88B31A5992@e2k3backend1.RCHSD.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F64@EMAIL.archildrens.org> I'm don't know of a CAP regulation but it isn't a good idea to store antibodies or tissue for that matter in a frost free freezer. It can dry out the antibodies or tissue if left for long periods of time. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breisch, Eric Sent: Tuesday, January 09, 2007 6:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP regulations regarding storage of antibodies in frost free refrigerator freezers Hi Histonet- Is anyone aware of new or existing CAP regulations regarding the use of frost free refrigerator/freezers for the storage of antibodies ie. IFA antibodies or IHC antibodies? Please advise if anyone has information pertaining to Histology and the above mentioned use of a refrigerator/freezer. Thank you, Eric A. Breisch, Ph.D. Clinical Anatomist Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy UCSD SOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From rjbuesa <@t> yahoo.com Wed Jan 10 08:27:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 10 08:27:38 2007 Subject: [Histonet] CAP regulations regarding storage of antibodies in frost free refrigerator freezers In-Reply-To: <43B97B4C402C2C44AAA2A8D2C86A88B31A5992@e2k3backend1.RCHSD.org> Message-ID: <560675.85098.qm@web61216.mail.yahoo.com> Eric: About antibodies storage I only know about the following regulations: 1-for refrigerator storage (nowadays all refrigerators are frost free) there has to be a log with daily temperature records, and the antibodies cannot be stored above their shelf expiration date; 2-aliquots of concentrated antibodies (for any type of procedure) can be stored in deep freezers; the only safety regulation is that they cannot be thawed and refrozen.There has to be also a log with the daily temperature. Ren? J. "Breisch, Eric" wrote: Hi Histonet- Is anyone aware of new or existing CAP regulations regarding the use of frost free refrigerator/freezers for the storage of antibodies ie. IFA antibodies or IHC antibodies? Please advise if anyone has information pertaining to Histology and the above mentioned use of a refrigerator/freezer. Thank you, Eric A. Breisch, Ph.D. Clinical Anatomist Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy UCSD SOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From pkarlisch <@t> psu.edu Wed Jan 10 08:28:18 2007 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Wed Jan 10 08:28:46 2007 Subject: [Histonet] CAP regulations regarding storage of antibodiesin frost free refrigerator freezers In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F64@EMAIL.archildrens.org> References: <43B97B4C402C2C44AAA2A8D2C86A88B31A5992@e2k3backend1.RCHSD.org> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F64@EMAIL.archildrens.org> Message-ID: <45A4B1B20200008C00034FE3@GWIA02.HERSHEYMED.NET> A long time ago I was told that you can NOT use a freezer to store antibodies or tissue unless it is NON- frost free. CAP actually cited those with frost free due to the temperature declining enough to melt ice and thus affect the storage of such samples. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Horn, Hazel V" 1/10/2007 9:10 AM >>> I'm don't know of a CAP regulation but it isn't a good idea to store antibodies or tissue for that matter in a frost free freezer. It can dry out the antibodies or tissue if left for long periods of time. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breisch, Eric Sent: Tuesday, January 09, 2007 6:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP regulations regarding storage of antibodies in frost free refrigerator freezers Hi Histonet- Is anyone aware of new or existing CAP regulations regarding the use of frost free refrigerator/freezers for the storage of antibodies ie. IFA antibodies or IHC antibodies? Please advise if anyone has information pertaining to Histology and the above mentioned use of a refrigerator/freezer. Thank you, Eric A. Breisch, Ph.D. Clinical Anatomist Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy UCSD SOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Jan 10 08:34:52 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Jan 10 08:35:17 2007 Subject: [Histonet] CAP regulations regarding storage of antibodiesin frost free refrigerator freezers In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F64@EMAIL.archildrens.org> Message-ID: The same goes for storing tissues in an auto-defrost cryostat!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: 10 January 2007 14:10 To: Breisch, Eric; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP regulations regarding storage of antibodiesin frost free refrigerator freezers I'm don't know of a CAP regulation but it isn't a good idea to store antibodies or tissue for that matter in a frost free freezer. It can dry out the antibodies or tissue if left for long periods of time. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breisch, Eric Sent: Tuesday, January 09, 2007 6:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP regulations regarding storage of antibodies in frost free refrigerator freezers Hi Histonet- Is anyone aware of new or existing CAP regulations regarding the use of frost free refrigerator/freezers for the storage of antibodies ie. IFA antibodies or IHC antibodies? Please advise if anyone has information pertaining to Histology and the above mentioned use of a refrigerator/freezer. Thank you, Eric A. Breisch, Ph.D. Clinical Anatomist Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy UCSD SOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maria.geraci-erck <@t> spcorp.com Wed Jan 10 08:53:01 2007 From: maria.geraci-erck <@t> spcorp.com (Geraci-Erck, Maria) Date: Wed Jan 10 08:53:12 2007 Subject: [Histonet] Kaposi Sarcoma lesion needed Message-ID: Dear Histonet, I am in need of a human Kaposi Sarcoma lesion that is formalin-fixed, paraffin embedded. Would anyone know where I could get one or purchase one? I have inquiries out to 3 companies and I am waiting to hear back. Any information would be useful. Thank you. Maria Maria Geraci-Erck Schering-Plough Research Institute Pathology, Immunohistochemistry 556 Morris Avenue, Bldg. S-12 Summit, New Jersey 07901-1002 Phone: (908) 473-4284 Fax: (908) 473-4420 maria.geraci-erck@spcorp.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From petepath <@t> yahoo.com Wed Jan 10 09:28:43 2007 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Jan 10 09:28:51 2007 Subject: [Histonet] Looking for microarray immuno control blocks Message-ID: <990822.4377.qm@web30401.mail.mud.yahoo.com> Can anyone recommend a vendor for microarray immuno control blocks. Thanks Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From Reuel.Cornelia <@t> tsrh.org Wed Jan 10 09:36:30 2007 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Wed Jan 10 09:37:15 2007 Subject: [Histonet] feedback on Leica 300 Message-ID: <45A4B39E020000C500003F51@MAIL.TSRH.ORG> Can anyone give me any comments about Leica ASP 300 tissue processor? it would be helpful in my decision to purchase this instrument. I would appreciate if vendors stay out of this and only users can give me their comments. Thank you very much. Reuel Cornelia Cellular Pathology Department Texas Scottish Rite Hospital Dallas, TX 75243 214-559-7766 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From slappycraw <@t> yahoo.com Wed Jan 10 09:42:42 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Jan 10 09:42:54 2007 Subject: [Histonet] EMA on decalcified mouse tibias Message-ID: <20070110154242.68737.qmail@web53611.mail.yahoo.com> Has anyone out there ever had any luck doing an EMA stain on decalcified mouse tibias, if so any info would be appreciated. Thanks --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From wia2005 <@t> med.cornell.edu Wed Jan 10 09:49:35 2007 From: wia2005 <@t> med.cornell.edu (William Ares) Date: Wed Jan 10 09:49:46 2007 Subject: [Histonet] vibrating microtome blades Message-ID: <7dad1aa6170d9.45a4c4bf@med.cornell.edu> My lab recently bought a Leica VT1000S vibrating microtome and as I was unhappy with the saphire blade that the model was offered with, we have been circulating through numerous brands of disposable metal blades. Does anyone have a suggestion regarding disposable blades for cutting fixed and live brain tissue? Thx in advance. William Ares Research Technician Labratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu From oshel1pe <@t> cmich.edu Wed Jan 10 10:30:00 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Jan 10 10:30:22 2007 Subject: [Histonet] vibrating microtome blades In-Reply-To: <7dad1aa6170d9.45a4c4bf@med.cornell.edu> References: <7dad1aa6170d9.45a4c4bf@med.cornell.edu> Message-ID: William, I had best results with Schick Platinum Plus injector blades. I don't know if they're still available. These are blades made for a shaving razor, and I don't know if they fit a Leica blade holder. They do fit the Vibratome. Phil >My lab recently bought a Leica VT1000S vibrating microtome and as I >was unhappy with the saphire blade that the model was offered with, >we have been circulating through numerous brands of disposable metal >blades. Does anyone have a suggestion regarding disposable blades >for cutting fixed and live brain tissue? Thx in advance. > >William Ares >Research Technician >Labratory of Molecular and Developmental Neuroscience >Weill Medical College of Cornell University >Tel: (212) 746 5056 >Email: wia2005@med.cornell.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From algranth <@t> u.arizona.edu Wed Jan 10 10:40:46 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Jan 10 10:40:58 2007 Subject: [Histonet] vibrating microtome blades In-Reply-To: <7dad1aa6170d9.45a4c4bf@med.cornell.edu> Message-ID: <4.3.2.7.2.20070110093943.00e06e40@algranth.inbox.email.arizona.edu> We just use the same blades that we use for cutting paraffin - except that we break them to fit in the holder of our vibratome. That would be Accu-edge blades. Andi At 10:49 AM 1/10/2007 -0500, William Ares wrote: >My lab recently bought a Leica VT1000S vibrating microtome and as I was >unhappy with the saphire blade that the model was offered with, we have >been circulating through numerous brands of disposable metal blades. Does >anyone have a suggestion regarding disposable blades for cutting fixed and >live brain tissue? Thx in advance. > >William Ares >Research Technician >Labratory of Molecular and Developmental Neuroscience >Weill Medical College of Cornell University >Tel: (212) 746 5056 >Email: wia2005@med.cornell.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Rcartun <@t> harthosp.org Wed Jan 10 10:54:34 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 10 10:55:04 2007 Subject: [Histonet] Charge for packaging slides for sendouts In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F48@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F48@sjhaexc02.sjha.org> Message-ID: <45A4D3FA0200007700003B1D@hcnwgwds01.hh.chs> I think I was the one who initiated this discussion. I need to summarize the responses, but I can tell you that there are labs that bill the patient (cash or charge card since insurance won't pay for this) for sending their slides out to another medical institution. We are thinking about charging the express shipping to the patient's charge card since we can no longer afford to absorb this cost. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Weems, Joyce" 01/09/07 6:22 PM >>> It seems that it we just recently had this discussion, but I was unable to find it by searching. I don't remember what the consensus was, but if I recall correctly, there is no CPT code that will allow us to charge a handling fee for recuts, packaging, and shipping cases. One more time... what are you doing in this regard? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From JEllin <@t> yumaregional.org Wed Jan 10 11:24:45 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Jan 10 11:25:02 2007 Subject: [Histonet] Charge for packaging slides for sendouts Message-ID: <29BE166A2CF48D459853F8EC57CD37E87E932E@EXCHANGECLUSTER.yumaregional.local> Here we currently use CPT code 99001 "Handling and/or conveyance of specimen for transfer from the patient in other than a physician's office to a laboratory." when we are shipping/packaging materials to be send out to another facility. As for recuts there is no code that I know of, but if there is let us know we are constantl doing recuts. Medicare bundles this code, but other insurance's will pay for this code. We also track this for work load accounting of how many outside tests are being out through our PIS database. Great for tracking time that is being spent on these proccess. If anyone has questions feel free to give me a call Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathologist Assistant Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From tkngflght <@t> yahoo.com Wed Jan 10 10:28:40 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Wed Jan 10 11:28:46 2007 Subject: [Histonet] CAP regulations regarding storage of antibodies infrost free refrigerator freezers In-Reply-To: <560675.85098.qm@web61216.mail.yahoo.com> Message-ID: <004301c734d4$636baaa0$6401a8c0@FSDESKTOP> Hi All-- An additional note about freezing antibodies-- Some of us used to suspend the outdate when aliquots were stored below -70. This hasn't been allowed for several years...THAT was a bummer. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From cmiller <@t> physlab.com Wed Jan 10 11:36:41 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jan 10 11:36:50 2007 Subject: [Histonet] (no subject) Message-ID: <000001c734dd$e3cc43e0$db01a8c0@plab.local> Anyone know where I can purchase diastase malt?? The saliva method isn't t working as well as it has in the past. I have spent over 30 mins looking for it online and I am getting no where. Product code and manufacturer would be perfect!. Thanks, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne From rjbuesa <@t> yahoo.com Wed Jan 10 11:45:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 10 11:45:44 2007 Subject: [Histonet] (no subject) In-Reply-To: <000001c734dd$e3cc43e0$db01a8c0@plab.local> Message-ID: <20070110174532.61678.qmail@web61223.mail.yahoo.com> The "saliva method" is not only unhealthy, but disgusting. I used to buy my malt diastase from Fisher (Fisher brand "maltin" Cat.No. D22-100) and prepared at 0.5 g diluted in diastase buffer (40 mL). Incubated in oven at 37?C x 1 hour. Ren? J. Cheri Miller wrote: Anyone know where I can purchase diastase malt?? The saliva method isn't t working as well as it has in the past. I have spent over 30 mins looking for it online and I am getting no where. Product code and manufacturer would be perfect!. Thanks, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From Heather.D.Renko <@t> osfhealthcare.org Wed Jan 10 12:28:57 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Jan 10 12:29:19 2007 Subject: [Histonet] freezer storage Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612C8F@pmc-rfd-mx01.intranet.osfnet.org> I agree with Hazel, I do not think it is a good idea. I can tell you that I was an inspector for CAP just last October and I do not remember there being any stipulations in regards to freezer storage. CAP will send you the checklist or it is available online for your facility. Good preparation though-you have to cover all bases with CAP. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From tim.morken <@t> thermofisher.com Wed Jan 10 12:40:17 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Jan 10 12:40:46 2007 Subject: [Histonet] (no subject) In-Reply-To: <000001c734dd$e3cc43e0$db01a8c0@plab.local> Message-ID: Cheri, Good idea, it is much better to use a manufactured chemical as it will allow you to make it the same every time. Look for Diastase or Amylase. At www.sigmaaldrich.com you will get this: Diastase from Aspergillus oryzae (1) CAS Number: 9000-92-4 09962 BioChemika, powder, white, amylase activity ?3500 U/g (Fluka) ?-Amylase from Aspergillus oryzae (1) CAS Number: 9001-19-8 86250 BioChemika, powder, ~1.5 units/mg (~0.2 U acc. to Willst?tter) (Fluka) Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, January 10, 2007 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Anyone know where I can purchase diastase malt?? The saliva method isn't t working as well as it has in the past. I have spent over 30 mins looking for it online and I am getting no where. Product code and manufacturer would be perfect!. Thanks, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab <@t> hyhc.com Wed Jan 10 12:52:52 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Wed Jan 10 12:47:50 2007 Subject: [Histonet] (no subject) Message-ID: We get ours from Poly Scientific. It comes as a set with the powder and the buffer solution that you mix just before use. The catalog number is S174P. Hope this helps, Dawn -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, January 10, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Anyone know where I can purchase diastase malt?? The saliva method isn't t working as well as it has in the past. I have spent over 30 mins looking for it online and I am getting no where. Product code and manufacturer would be perfect!. Thanks, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From brooke <@t> premierlab.com Wed Jan 10 13:32:28 2007 From: brooke <@t> premierlab.com (Brooke Davidsen) Date: Wed Jan 10 13:21:55 2007 Subject: [Histonet] CMet Pan (C28) Message-ID: <000101c734ee$103d1d90$0f00a8c0@domain.Premier> I'm wondering if anyone of you out there has used the C-28 (CMet Pan) from SantaCruz. I've tried it and can't seem to get any staining. I'm doing a heated retrieval and using a polymer as my secondary antibody. Does anybody have any suggestions? Thanks, Brooke. From TJJ <@t> Stowers-Institute.org Wed Jan 10 13:32:50 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jan 10 13:33:24 2007 Subject: [Histonet] Need technique information Message-ID: Hi all, I have some slides of cultured cells which are immunostained, fixed in 4% PFA and sorted through a FACS. The sorted cells are then dropped onto glass slides for H&E and eventually IHC and ISH staining. They have dropped some cell suspension on to a plus-slide and air dried at 60 degrees C, then post-fixed in formalin and air dried again. The morphology isn't very good and the cytoplasm doesn't show up at all on H&E. Does anybody have a protocol for doing either cytospins (may not be possible, only 2000 cells total!) or cell suspension dried slides to get better morphology? I believe they probably don't need heat to dry them. I also believe they don't need an additional post-fix, but if they do one, to keep the slide wet afterward (no additional air-drying step) and begin whatever staining technique follows. Thanks in advance for any suggestions! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Heather.D.Renko <@t> osfhealthcare.org Wed Jan 10 13:50:02 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Jan 10 13:50:17 2007 Subject: [Histonet] Block identifiers Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From hlob <@t> emory.edu Wed Jan 10 13:57:49 2007 From: hlob <@t> emory.edu (Heinrich Lob) Date: Wed Jan 10 13:57:59 2007 Subject: [Histonet] cd8 Message-ID: <9e5d1b400701101157v14a78adg901d176946e73707@mail.gmail.com> Hi, does anybody know where I could find CD8 anti-mouse for IHC-paraffin? Heinrich From SDrew <@t> uwhealth.org Wed Jan 10 14:18:08 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Jan 10 14:18:20 2007 Subject: [Histonet] OCT 3/4 Message-ID: Is anyone running a particular clone or vendor's OCT 3/4 on human formalin-fixed paraffin-embedded tissues that they'd like to rave about? Also, are there known pitfalls in working up this antibody? For what types of cases is this antibody being ordered? Thank you for any input you've time to give! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From settembr <@t> umdnj.edu Wed Jan 10 14:25:35 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jan 10 14:26:31 2007 Subject: [Histonet] cd8 Message-ID: Hello Heinrich, I use CD8 made in mouse from Dako, cat.# M7103 I use it on FFPE human tissue and I pretreat with their Target Retreival Solution. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Heinrich Lob 01/10/07 2:57 PM >>> Hi, does anybody know where I could find CD8 anti-mouse for IHC-paraffin? Heinrich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 10 14:48:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 10 14:48:40 2007 Subject: [Histonet] cd8 In-Reply-To: <9e5d1b400701101157v14a78adg901d176946e73707@mail.gmail.com> Message-ID: <575360.9977.qm@web61217.mail.yahoo.com> Heinrich: I used CD8 from NovoCastra at 1:30 dilution after HIER at pH8, tonsil controls, DAKO detection systems and autostainer. It worked fine Ren? J. Heinrich Lob wrote: Hi, does anybody know where I could find CD8 anti-mouse for IHC-paraffin? Heinrich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From EWURDAK <@t> CSBSJU.EDU Wed Jan 10 15:14:06 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Wed Jan 10 15:14:12 2007 Subject: [Histonet] (no subject) In-Reply-To: <000001c734dd$e3cc43e0$db01a8c0@plab.local> Message-ID: We got ours from Fisher. Sigma carries alpha amylase from barley malt. I think that's the same. Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 1/10/07 11:36 AM, "Cheri Miller" wrote: > > > Anyone know where I can purchase diastase malt?? The saliva method isn't t > working as well as it has in the past. I have spent over 30 mins looking for > it online and I am getting no where. Product code and manufacturer would be > perfect!. Thanks, Cheri > > > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 10 15:16:47 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 10 15:16:55 2007 Subject: [Histonet] diastase digestion Message-ID: <219023.52629.qm@web31309.mail.mud.yahoo.com> Note: forwarded message attached. From akemiat3377 <@t> yahoo.com Wed Jan 10 15:21:00 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 10 15:21:15 2007 Subject: [Histonet] diastase digestion Message-ID: <980183.88609.qm@web31306.mail.mud.yahoo.com> Hi, I was one of those dinosaurs. Years ago, I used to use the old spit method too. Nothing like natural enzyme digestion. I was taught that method by Bonnie Proctor from Providence Hospital, Portland OR in 1976. She was Navy trained. It worked great, but the green stuff after lunch got to be a little too much for the younger histo tech's. Plus, it's not exactly a set standard protocol, since everyone has different enzyme make-up. You also had to rinse it off for a long period of time because it dried out on the parameter. Diastase malt didn't seem to work very well after years of inconsistent results. Our lab switched to amylase digestion. We got more consistent results. Check with sigma-aldrich.com Good Luck, Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com From rennie11505 <@t> yahoo.com Wed Jan 10 15:39:26 2007 From: rennie11505 <@t> yahoo.com (Adrienne Anderson) Date: Wed Jan 10 15:39:36 2007 Subject: [Histonet] Preserving mucus membrane Message-ID: <197204.93441.qm@web37009.mail.mud.yahoo.com> Hello, Does anyone know of a good fixative for preserving mucus membranes of the gut--are there any? Thanks, Adrienne Mazzante Histotechnology Student Medical University of South Carolina --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From nbhatia <@t> dermatology.wisc.edu Wed Jan 10 15:58:31 2007 From: nbhatia <@t> dermatology.wisc.edu (Neehar Bhatia) Date: Wed Jan 10 15:58:41 2007 Subject: [Histonet] (no subject) Message-ID: <20070110155831109.00000001928@derm-HF55T81> Generator Microsoft Word 11 (filtered medium) Hello Everyone, I have been trying to stain paraffin embedded skin tissue from transgenic mice for HA tag expression to no avail. Has anyone been successful for this. These mice also express b-gal. Can I stain these sections for b-gal expression using the antibody. If so what method of antigen retrieval should I use as heat will destroy this enzyme. Please help Thanks Neehar Bhatia Neehar Bhatia, Ph.D Assistant Scientist, Department of Dermatology, University of Wisconsin, 445 Henry Mall, Rm 302, Madison WI 53706 Ph 608.262.3239 Fax 608.263.2919 From jnocito <@t> satx.rr.com Wed Jan 10 16:07:07 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 10 16:08:55 2007 Subject: [Histonet] CAP regulations regarding storage of antibodiesin frost free refrigerator freezers References: <43B97B4C402C2C44AAA2A8D2C86A88B31A5992@e2k3backend1.RCHSD.org> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F64@EMAIL.archildrens.org> <45A4B1B20200008C00034FE3@GWIA02.HERSHEYMED.NET> Message-ID: <022501c73503$ac16fc30$31614542@yourxhtr8hvc4p> This is my big beef with CAP. When I was at the AFIP from 1986-1991, there were frozen antibodies that were aliquoted in 1982 at -70 C. When I left in 1991, we were still using those antibodies with excellent results. Then the question came up about revalidating the reagents. When I went through a CLIA inspection, the inspector cited me. I told her that every time I run a patient, there is always a known positive control run with the patient. If it didn't work, then I would repeat it. It was tough explaining that to her because she was long time Chemistry tech, which is understandable, but give me a break. Is it Friday yet? Joe ----- Original Message ----- From: "Patricia Karlisch" To: "Hazel V Horn" ; "Eric Breisch" ; Sent: Wednesday, January 10, 2007 8:28 AM Subject: RE: [Histonet] CAP regulations regarding storage of antibodiesin frost free refrigerator freezers >A long time ago I was told that you can NOT use a freezer to store > antibodies or tissue unless it is NON- frost free. CAP actually cited > those with frost free due to the temperature declining enough to melt > ice and thus affect the storage of such samples. Pat > > Pat Karlisch > Supervisor, Histology, Pathology and Laboratory Medicine > Penn State Milton S. Hershey Medical Center > Mail Code H179 > Hershey, PA 17033 > Phone (717) 531-6072 > Fax: (717) 531- 7741 > email: pkarlisch@psu.edu > > *****E-Mail Confidentiality Notice***** > This message (including any attachments) contains information intended > for a specific individual(s) and purpose that may be privileged, > confidential or otherwise protected from disclosure pursuant to > applicable law. Any inappropriate use, distribution or copying of the > message is strictly prohibited and may subject you to criminal or civil > penalty. If you have received this transmission in error, please reply > to the sender indicating this error and delete the transmission from > your system immediately. > > >>>> "Horn, Hazel V" 1/10/2007 9:10 AM >>> > > I'm don't know of a CAP regulation but it isn't a good idea to store > antibodies or tissue for that matter in a frost free freezer. It can > dry out the antibodies or tissue if left for long periods of time. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breisch, > Eric > Sent: Tuesday, January 09, 2007 6:21 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP regulations regarding storage of antibodies in > frost free refrigerator freezers > > Hi Histonet- > > > > Is anyone aware of new or existing CAP regulations > regarding > the use of frost free refrigerator/freezers for the storage of > antibodies ie. IFA antibodies or IHC antibodies? Please advise if > anyone > has information pertaining to Histology and the above mentioned use of > a > refrigerator/freezer. > > > > Thank you, > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > UCSD SOM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------------ > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, you > are hereby notified that any dissemination, distribution or copying of > this communication is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Wed Jan 10 16:22:19 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Wed Jan 10 16:22:33 2007 Subject: [Histonet] diastase digestion In-Reply-To: <980183.88609.qm@web31306.mail.mud.yahoo.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12728@VHAV20MSGA3.v20.med.va.gov> Dinosaur? I graduated from my HT program in 2000. I am but a puppy to most of you big dogs. However, In the past 6 years of working as a bench tech, I have found no better digestion method then spitting on the slide. As far as "the green stuff after lunch being too much for the youngsters"... try an autopsy with necrotic bowel after lunch, or why not before lunch. We work in pathology and some of us think spit is gross? Maybe now is a time to re-evaluate your career choice while you are still young. I was in diapers when Bonnie Proctor taught you this most excellent method. I used it today. Is there a problem with this as far as CAP is concerned? I have it in my protocol. Even though we all have a different enzyme make up, I have yet to see different end results from different people's spit. So, as long as I am not breaking some cardinal rule I say... haccc toooowie... :) Jason Wiese, BS, HT(ASCP) Histology/Cytology Roseburg OR 541-440-1000 Ext. 44751 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, January 10, 2007 1:21 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase digestion Hi, I was one of those dinosaurs. Years ago, I used to use the old spit method too. Nothing like natural enzyme digestion. I was taught that method by Bonnie Proctor from Providence Hospital, Portland OR in 1976. She was Navy trained. It worked great, but the green stuff after lunch got to be a little too much for the younger histo tech's. Plus, it's not exactly a set standard protocol, since everyone has different enzyme make-up. You also had to rinse it off for a long period of time because it dried out on the parameter. Diastase malt didn't seem to work very well after years of inconsistent results. Our lab switched to amylase digestion. We got more consistent results. Check with sigma-aldrich.com Good Luck, Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From EWURDAK <@t> CSBSJU.EDU Wed Jan 10 16:33:14 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Wed Jan 10 16:33:21 2007 Subject: [Histonet] Staining plant tissues Message-ID: Hello Histonetters, I am looking for a good stain for plant tissue. A couple of years ago we tried Johansen's quadruple stain as described in Gray's Handbook of Basic Microtechnique, but we were not satisfied with the results. We fixed the tissues in FAA (95% ethanol, glacial acetic acid, formalin). Thank you in advance for any advice you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 From llewllew <@t> shaw.ca Wed Jan 10 16:35:21 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Jan 10 16:37:11 2007 Subject: [Histonet] diastase digestion References: <70EEF3D43B3C164C94037D811B2BE193E12728@VHAV20MSGA3.v20.med.va.gov> Message-ID: <005d01c73507$9d003c30$6400a8c0@yourlk4rlmsu> People who are sick, with tuberculosis for instance, who spit on slides can pass the disease around. That's the major problem with it. A replacement is hog alpha amylase from Sigma. A small amount (cover a dime thinly) in 10 mL distilled water for 30 mins at RT is all that is need for removing glycogen from a PAS. I used that for 30 years after giving up spitting at my mother's insistance. Bryan Llewellyn ----- Original Message ----- From: "Wiese, Jason VHAROS" To: "Akemi Allison-Tacha" ; Sent: Wednesday, January 10, 2007 2:22 PM Subject: RE: [Histonet] diastase digestion Dinosaur? I graduated from my HT program in 2000. I am but a puppy to most of you big dogs. However, In the past 6 years of working as a bench tech, I have found no better digestion method then spitting on the slide. As far as "the green stuff after lunch being too much for the youngsters"... try an autopsy with necrotic bowel after lunch, or why not before lunch. We work in pathology and some of us think spit is gross? Maybe now is a time to re-evaluate your career choice while you are still young. I was in diapers when Bonnie Proctor taught you this most excellent method. I used it today. Is there a problem with this as far as CAP is concerned? I have it in my protocol. Even though we all have a different enzyme make up, I have yet to see different end results from different people's spit. So, as long as I am not breaking some cardinal rule I say... haccc toooowie... :) Jason Wiese, BS, HT(ASCP) Histology/Cytology Roseburg OR 541-440-1000 Ext. 44751 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, January 10, 2007 1:21 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase digestion Hi, I was one of those dinosaurs. Years ago, I used to use the old spit method too. Nothing like natural enzyme digestion. I was taught that method by Bonnie Proctor from Providence Hospital, Portland OR in 1976. She was Navy trained. It worked great, but the green stuff after lunch got to be a little too much for the younger histo tech's. Plus, it's not exactly a set standard protocol, since everyone has different enzyme make-up. You also had to rinse it off for a long period of time because it dried out on the parameter. Diastase malt didn't seem to work very well after years of inconsistent results. Our lab switched to amylase digestion. We got more consistent results. Check with sigma-aldrich.com Good Luck, Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Jan 10 16:40:41 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jan 10 16:42:43 2007 Subject: [Histonet] diastase digestion Message-ID: Yep, You can still spit if you like but there is an alternative. See: V-M. Mangan, V. Farago, M. Kelly, and A. F. Henwood (2002) " An Amylase Reagent with a Long Shelf Life for the Removal of Glycogen from Tissue Sections" J Histotechnol. 25(3): 153-4. Apart from blowing my lab's own trumpet, this procedure digests glycogen in 10 minutes at room temp and the solution lasts several months at 4oC: Amylase Reagent Warning: Harmful, contains azide - see MSDS Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,) 1g Oxoid PBS Tablets (Cat No BR14a) 1 tablet Distilled water 100ml Sodium Azide 0.1g This solution, once prepared is stored at 4oC when not in use. A recycled antibody dropper bottle (often used in commercial immunoperoxidase kits) is useful for storage and application. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Thursday, 11 January 2007 9:22 AM To: Akemi Allison-Tacha; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] diastase digestion Dinosaur? I graduated from my HT program in 2000. I am but a puppy to most of you big dogs. However, In the past 6 years of working as a bench tech, I have found no better digestion method then spitting on the slide. As far as "the green stuff after lunch being too much for the youngsters"... try an autopsy with necrotic bowel after lunch, or why not before lunch. We work in pathology and some of us think spit is gross? Maybe now is a time to re-evaluate your career choice while you are still young. I was in diapers when Bonnie Proctor taught you this most excellent method. I used it today. Is there a problem with this as far as CAP is concerned? I have it in my protocol. Even though we all have a different enzyme make up, I have yet to see different end results from different people's spit. So, as long as I am not breaking some cardinal rule I say... haccc toooowie... :) Jason Wiese, BS, HT(ASCP) Histology/Cytology Roseburg OR 541-440-1000 Ext. 44751 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, January 10, 2007 1:21 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase digestion Hi, I was one of those dinosaurs. Years ago, I used to use the old spit method too. Nothing like natural enzyme digestion. I was taught that method by Bonnie Proctor from Providence Hospital, Portland OR in 1976. She was Navy trained. It worked great, but the green stuff after lunch got to be a little too much for the younger histo tech's. Plus, it's not exactly a set standard protocol, since everyone has different enzyme make-up. You also had to rinse it off for a long period of time because it dried out on the parameter. Diastase malt didn't seem to work very well after years of inconsistent results. Our lab switched to amylase digestion. We got more consistent results. Check with sigma-aldrich.com Good Luck, Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From valleygal <@t> aol.com Wed Jan 10 16:43:14 2007 From: valleygal <@t> aol.com (valleygal@aol.com) Date: Wed Jan 10 16:43:29 2007 Subject: [Histonet] Staining plant tissues In-Reply-To: References: Message-ID: <8C9031FD9305AB9-17C0-1E5D@WEBMAIL-DC13.sysops.aol.com> Depends on what you are staining for - are you looking for a good general stain? There are several protocols in the Ruzin book, Plant Microtechnique and Microscopy. Andi Grantham -----Original Message----- From: EWURDAK@CSBSJU.EDU To: histonet@lists.utsouthwestern.edu Sent: Wed, 10 Jan 2007 3:33 PM Subject: [Histonet] Staining plant tissues Hello Histonetters, I am looking for a good stain for plant tissue. A couple of years ago we tried Johansen's quadruple stain as described in Gray's Handbook of Basic Microtechnique, but we were not satisfied with the results. We fixed the tissues in FAA (95% ethanol, glacial acetic acid, formalin). Thank you in advance for any advice you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Jason.Wiese <@t> va.gov Wed Jan 10 17:10:54 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Wed Jan 10 17:11:03 2007 Subject: [Histonet] diastase digestion In-Reply-To: <005d01c73507$9d003c30$6400a8c0@yourlk4rlmsu> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12729@VHAV20MSGA3.v20.med.va.gov> If you have tuberculosis, I hope you are not working in my laboratory... you can pass it around anyway. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Wednesday, January 10, 2007 2:35 PM To: Histonet Subject: Re: [Histonet] diastase digestion People who are sick, with tuberculosis for instance, who spit on slides can pass the disease around. That's the major problem with it. A replacement is hog alpha amylase from Sigma. A small amount (cover a dime thinly) in 10 mL distilled water for 30 mins at RT is all that is need for removing glycogen from a PAS. I used that for 30 years after giving up spitting at my mother's insistance. Bryan Llewellyn ----- Original Message ----- From: "Wiese, Jason VHAROS" To: "Akemi Allison-Tacha" ; Sent: Wednesday, January 10, 2007 2:22 PM Subject: RE: [Histonet] diastase digestion Dinosaur? I graduated from my HT program in 2000. I am but a puppy to most of you big dogs. However, In the past 6 years of working as a bench tech, I have found no better digestion method then spitting on the slide. As far as "the green stuff after lunch being too much for the youngsters"... try an autopsy with necrotic bowel after lunch, or why not before lunch. We work in pathology and some of us think spit is gross? Maybe now is a time to re-evaluate your career choice while you are still young. I was in diapers when Bonnie Proctor taught you this most excellent method. I used it today. Is there a problem with this as far as CAP is concerned? I have it in my protocol. Even though we all have a different enzyme make up, I have yet to see different end results from different people's spit. So, as long as I am not breaking some cardinal rule I say... haccc toooowie... :) Jason Wiese, BS, HT(ASCP) Histology/Cytology Roseburg OR 541-440-1000 Ext. 44751 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, January 10, 2007 1:21 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase digestion Hi, I was one of those dinosaurs. Years ago, I used to use the old spit method too. Nothing like natural enzyme digestion. I was taught that method by Bonnie Proctor from Providence Hospital, Portland OR in 1976. She was Navy trained. It worked great, but the green stuff after lunch got to be a little too much for the younger histo tech's. Plus, it's not exactly a set standard protocol, since everyone has different enzyme make-up. You also had to rinse it off for a long period of time because it dried out on the parameter. Diastase malt didn't seem to work very well after years of inconsistent results. Our lab switched to amylase digestion. We got more consistent results. Check with sigma-aldrich.com Good Luck, Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MTitford <@t> aol.com Wed Jan 10 20:41:18 2007 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Wed Jan 10 20:41:37 2007 Subject: [Histonet] Botanical stains Message-ID: <329.10ff48af.32d6fdce@aol.com> Elizabeth Wurdak asks about botanical stains: Many years ago, (many, many years ago !) when I attended basic laboratory technique classes in London, the standard stain for botany was, I think, Safranine and light green. The sections were a little thicker and a delight to look at. I am sure you could easily find the method in the Internet. Also, strangely, alcian blue and safranine can be used too. We used to hand sections with a cut throat razor....... Michael Titford USA Pathology Mobile AL USA From akemiat3377 <@t> yahoo.com Wed Jan 10 22:05:06 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 10 22:05:16 2007 Subject: [Histonet] Botanical stains In-Reply-To: <329.10ff48af.32d6fdce@aol.com> Message-ID: <824720.10136.qm@web31303.mail.mud.yahoo.com> Dear Elizabeth, If you really want to hear it from the horses mouth, talk to Dr. Richard Horobin. He is a wonderful man, who truely loves histology! He probably has more degrees than a thermometer! He is the editor for the Biological Stain Commision's journal. His e-mail is: richardhorobin@tomcroy.co.uk Give him my Best Regards & Next Time lets Share a Pint! Akemi --- MTitford@aol.com wrote: > Elizabeth Wurdak asks about botanical stains: > > Many years ago, (many, many years ago !) when I > attended basic laboratory > technique classes in London, the standard stain for > botany was, I think, > Safranine and light green. The sections were a > little thicker and a delight to look > at. I am sure you could easily find the method in > the Internet. Also, > strangely, alcian blue and safranine can be used > too. We used to hand sections > with a cut throat razor....... > > Michael Titford > USA Pathology > Mobile AL USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Jan 11 01:42:36 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Jan 11 01:42:47 2007 Subject: Fwd: RE: [Histonet] diastase digestion Message-ID: <598233.48959.qm@web31309.mail.mud.yahoo.com> --- Akemi Allison-Tacha wrote: > Date: Wed, 10 Jan 2007 16:41:26 -0800 (PST) > From: Akemi Allison-Tacha > Subject: RE: [Histonet] diastase digestion > To: "Wiese, Jason VHAROS" > CC: dave@biocare.net > > Hi Jason, > > Just a heads-up & true clarification. Murray > started > a couple of years ago as a salesman and was not on > the > foundation team. > > Roy Yih is the President & CEO of Biocare. Roy's > father is Chairman of the board. Roy is the person > who now has the long hair. They are chinese not > japanese. The executive VP & CFO is Gene Casagnini. > > These 3 men's money started the company. > > The VP & Director of IHC is Dr. David Tacha. I used > to be the Director of Histology & TMA until 24/7 > took > it's toll and left in 2005. David developed all the > IHC products through his genious, as well as several > instruments. I developed all the H&E and SS > products. > R&D started in 1997 at the old Walnut Creek office > with just a handfull of people on the team. I > started > in 1998. We were lucky to make $5,000 in sales a > month. It took a team to make Biocare what it is > today. I hope they don't forget that. > > Akemi > --- "Wiese, Jason VHAROS" > wrote: > > > Hey thanks for the hello Akemi! > > > > > > > > It is a crazy small world sometimes. Wow... I > love > > the Portland > > Saturday Market! I just met everyone from Biocare > > Medical at the NSH in > > Phoenix. I actually interviewed with David > Tacha... > > well at least I > > thought I did. Perhaps I am confused. They guy I > > met with was Murray, > > but he introduced me to the CEO... I thought his > > name was David. He was > > a younger Asian guy, I believe Japanese, with > long > > hair like a rock > > star or something. He said that he and his father > > had started the > > company with their own money and made it, "what it > > is today." Perhaps I > > met the junior Tacha? Anyway, he was a great guy. > > > Straight forward no > > BS. I would be honored to work for someone with > his > > attitude. He > > offered me a job on the spot, but I am reluctant > to > > leave "god's > > country" to move to crowded California... not to > > mention a pay cut to > > start. I would have to give up my 5 bedroom > > farmhouse on acres of land > > to afford a studio apartment. Not now... maybe > not > > ever... > > > > > > > > Anyway, after returning from Phoenix, I decided to > > stay in Oregon. I > > have lived all over, and I have yet to find a > place > > I feel more at home. > > I truly love it here, and I have turned down jobs > > that would pay me > > twice as much, just so I can stay where I am. I > > take my PA medical > > board exam February 27th, and I am excited about > > that. The only problem > > is that in order to reap the benefits I may have > to > > move. Oh well, I > > guess they can never take the PA certification > away > > once I have it. > > Maybe some day in the future I will find the > perfect > > HT/PA job. Till > > then... I'll be right here. :-) > > > > > > > > I mean absolutely no disrespect by the term "big > > dog", it's just > > something I here quite a bit. It is actually a > > compliment. I try to > > learn something from everyone. There are people > who > > have been in this > > business for decades, and they have tons of valid > > information. However, > > people entering school right now are learning the > > newest technology, and > > they are learning from the mistakes of those who > > came before. As you > > said, there is something to be learned from > > everyone. By the way, I > > have often taken a break in the middle of an > autopsy > > to have lunch, and > > then went back to it. I think spit is about the > > nicest thing we > > encounter in our professions. > > > > > > > > Well, I better get back to work. Hope you have an > > awesome day!!!! > > > > > > > > Jason > > > > > > > > ________________________________ > > > > From: Akemi Allison-Tacha > > [mailto:akemiat3377@yahoo.com] > > Sent: Wednesday, January 10, 2007 3:02 PM > > To: Wiese, Jason VHAROS > > Subject: RE: [Histonet] diastase digestion > > > > > > > > Hi Jason, > > > > > > > > Good to hear your feed back. Great to hear you > have > > a good sense of > > humor from a fellow Oregonian. Some of the people > > in this field haven't > > had your exposure and most likely never will. > > Everyone has a different > > reason for getting into this crazy profession. > > > > > > > > I miss Oregon, it's God's country!. My ex-husband > > Dr. David Tacha of > > Biocare Medical, were from Oregon. I was one of > the > > founders of the > > Portland Saturday Market. I worked up on Pill > Hill, > > Emanuel Hospital, > > as well as setting-up a private derm lab. I > served > > as President of the > > OR Histology Society in 79 & 82 and brought Lee > > Luna, Dezna Sheehan & > > Jules Elias out as speakers. I don't know if I > > consider myself as a"Big > > Dog" I feel that, we all have something to learn > > from each other and > > sometimes it's the puppy that can teach us a thing > > or two. We need to > > have a mind like a parachute. "OPEN" > > > > > > > > I actually took histomicrotechnic at Tokyo > > University from 1966-68. My > > father was stationed in Japan at that time. I > > worked after classes at > > the base hospital. Viet Nam was going on, so I > was > > exposed to quite a > > bit of gruesome stuff. I never thought I would > end > > up in this field. > > > > > > > > After coming back to the states in 1968, I did my > > residency at > > Binghamton General Hospital in NY. Talk about > > exposure! I assisted in > > autopsies too before and after lunch. > > > > > > > > > > > > Best Wishes, > > > > Akemi > > > > > > > > > > > > "Wiese, Jason VHAROS" wrote: > > > > Dinosaur? I graduated from my HT program in 2000. > I > > am but a > > puppy to > > most of you big dogs. However, In the past 6 > years > > of working as > > a > > bench tech, I have found no better digestion > method > > then > > spitting on the > > slide. As far as "the green stuff after lunch > being > > too much for > > the > > youngsters"... try an autopsy with necrotic bowel > > after lunch, > > or why > > not before lunch. > > > > We work in pathology and some of us think spit is > > gross? Maybe > > now is a > > time to re-evaluate your career choice while you > > are still > > young. I was > > in diapers when Bonnie Proctor taught you this > most > > excellent > > method. I > > used it today. Is there a problem with this as > far > > as CAP is > > concerned? > > I have it in my protocol. Even though we all have > a > > different > > enzyme > > make up, I have yet to see different end results > > from different > > people's > > spit. So, as long as I am not breaking some > > cardinal rule I > > say... > > haccc toooowie... :) > > > > > === message truncated === > > From sohail_e <@t> yahoo.com Thu Jan 11 04:36:50 2007 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Thu Jan 11 04:37:00 2007 Subject: [Histonet] Chemicals that lower the activity of antibodies Message-ID: <584486.32211.qm@web39503.mail.mud.yahoo.com> Hello Usually, the labs are full of differnt chemicals which may have bad effects on the activity of antibodies. I am interested to know a list of chemicals which may lower the activity of commercillay preapred antibodies. Thanks Allis --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From DOOLEEO <@t> shands.ufl.edu Thu Jan 11 09:07:27 2007 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Thu Jan 11 09:08:23 2007 Subject: [Histonet] YAP1 Message-ID: Dear Histonetters, I have been working with Rabbit polyclonal to YAP1 from ABCAM. I have tried two breast cancer specimens and one live tumor and have not seen any staining of the paraffin sections. I used antigen retrieval and a 1:20 dilution of the primary antibody. Is there any special tricks to make this antibody work? Has anyone done this on an automated stainer? Thanks for any help in advance Elaine Dooley HTL Shands Teaching Hospital 352-265-0111 ext 72117 From mcauliff <@t> umdnj.edu Thu Jan 11 09:21:48 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jan 11 09:21:48 2007 Subject: [Histonet] Preserving mucus membrane In-Reply-To: <197204.93441.qm@web37009.mail.mud.yahoo.com> References: <197204.93441.qm@web37009.mail.mud.yahoo.com> Message-ID: <45A6560C.2050903@umdnj.edu> Hi Adrienne: Any good (buffered formalin, Bouin's, formalin-alcohol-acetic acid) fixative should be fine. I would use a pipette or a syringe to inject fixative into the lumen of the gut (and wash out partially digested food at the same time). Geoff Adrienne Anderson wrote: >Hello, > Does anyone know of a good fixative for preserving mucus membranes of the gut--are there any? > > Thanks, > > Adrienne Mazzante > Histotechnology Student > Medical University of South Carolina > > >--------------------------------- >Any questions? Get answers on any topic at Yahoo! Answers. Try it now. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Thu Jan 11 10:46:09 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 11 10:46:18 2007 Subject: [Histonet] Staining plant tissues In-Reply-To: Message-ID: <20070111164609.94031.qmail@web61224.mail.yahoo.com> Try Maller's hematoxyline combined with fast green and safranine. Ren? J. "Wurdak, Elizabeth" wrote: Hello Histonetters, I am looking for a good stain for plant tissue. A couple of years ago we tried Johansen's quadruple stain as described in Gray's Handbook of Basic Microtechnique, but we were not satisfied with the results. We fixed the tissues in FAA (95% ethanol, glacial acetic acid, formalin). Thank you in advance for any advice you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Thu Jan 11 10:52:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 11 10:53:01 2007 Subject: [Histonet] Chemicals that lower the activity of antibodies In-Reply-To: <584486.32211.qm@web39503.mail.mud.yahoo.com> Message-ID: <20070111165252.21541.qmail@web61221.mail.yahoo.com> The worst are chlorine derivatives. I found that out "the hard way" when my incubation chamber (when I did IHC manually) was left contaminated after the end of the week clean-up. Ren? J. sohail ejaz wrote: Hello Usually, the labs are full of differnt chemicals which may have bad effects on the activity of antibodies. I am interested to know a list of chemicals which may lower the activity of commercillay preapred antibodies. Thanks Allis --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From patrick_leung <@t> merck.com Thu Jan 11 11:17:40 2007 From: patrick_leung <@t> merck.com (Leung, Patrick) Date: Thu Jan 11 11:18:14 2007 Subject: [Histonet] Bone adhesion problems after HIER Message-ID: <4D1A82B94D90CC449EC8395E1DCF0C090C51FB@usctmx1134.merck.com> Hi, I am having problems with cortical bone detaching from the slide during the antigen retrieval step. The samples I am staining are paraffin embedded mouse tibia. I am currently using Superfrost+ slides. The AR is with citrate buffer (pH6.1) from Dako performed in a waterbath for 15 minutes. The slides are baked overnight at 59C prior to deparaffinizing. I have tried using a steamer and microwave, however I still ended up with the same results. In addition Superfrost GOLD slides did not help in keeping the bone on. I found that performing HIER at 70C for three hrs as suggested in the archives to be ineffective in exposing my antigen (although the bone did stay attached). Do you have any suggestions? Thanks, Patrick ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From liz <@t> premierlab.com Thu Jan 11 11:34:23 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 11 11:25:27 2007 Subject: [Histonet] Bone adhesion problems after HIER In-Reply-To: <4D1A82B94D90CC449EC8395E1DCF0C090C51FB@usctmx1134.merck.com> Message-ID: <000001c735a6$bc3b13a0$0d00a8c0@domain.Premier> Patrick Its very difficult to maintain section adherence of bone with HIER techniques. We try to use enzyme digestion methods over HEIR when ever we can. What antibody are you looking at? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leung, Patrick Sent: Thursday, January 11, 2007 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone adhesion problems after HIER Hi, I am having problems with cortical bone detaching from the slide during the antigen retrieval step. The samples I am staining are paraffin embedded mouse tibia. I am currently using Superfrost+ slides. The AR is with citrate buffer (pH6.1) from Dako performed in a waterbath for 15 minutes. The slides are baked overnight at 59C prior to deparaffinizing. I have tried using a steamer and microwave, however I still ended up with the same results. In addition Superfrost GOLD slides did not help in keeping the bone on. I found that performing HIER at 70C for three hrs as suggested in the archives to be ineffective in exposing my antigen (although the bone did stay attached). Do you have any suggestions? Thanks, Patrick ---------------------------------------------------------------------------- -- Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ---------------------------------------------------------------------------- -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From LSebree <@t> uwhealth.org Thu Jan 11 11:25:57 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Jan 11 11:26:03 2007 Subject: [Histonet] Bone adhesion problems after HIER In-Reply-To: <4D1A82B94D90CC449EC8395E1DCF0C090C51FB@usctmx1134.merck.com> Message-ID: Patrick, Since you had no bone detachment at 70 degrees, but also no staining, I would start there with an overnight AR at 70 degrees. Then if that still doesn't work I'd slowly increase either the time or temperature to the point where you get staining and still have tissue adherence. Just my thoughts, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leung, Patrick Sent: Thursday, January 11, 2007 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone adhesion problems after HIER Hi, I am having problems with cortical bone detaching from the slide during the antigen retrieval step. The samples I am staining are paraffin embedded mouse tibia. I am currently using Superfrost+ slides. The AR is with citrate buffer (pH6.1) from Dako performed in a waterbath for 15 minutes. The slides are baked overnight at 59C prior to deparaffinizing. I have tried using a steamer and microwave, however I still ended up with the same results. In addition Superfrost GOLD slides did not help in keeping the bone on. I found that performing HIER at 70C for three hrs as suggested in the archives to be ineffective in exposing my antigen (although the bone did stay attached). Do you have any suggestions? Thanks, Patrick ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbullard1231 <@t> yahoo.com Thu Jan 11 12:12:25 2007 From: tbullard1231 <@t> yahoo.com (Tara Bullard) Date: Thu Jan 11 12:12:39 2007 Subject: [Histonet] fluorescent mouse to mouse Message-ID: <934490.25754.qm@web50915.mail.yahoo.com> Hello All, I am new to the list. I have 2 separate questions which I will spost separately. The first question- is there a good kit for mouse to mouse fluorescent immunohistochemistry? Thank you, Tara --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From tbullard1231 <@t> yahoo.com Thu Jan 11 12:16:29 2007 From: tbullard1231 <@t> yahoo.com (Tara Bullard) Date: Thu Jan 11 12:16:40 2007 Subject: [Histonet] salivary gland IF Message-ID: <14363.23249.qm@web50910.mail.yahoo.com> Hello, My second question- our lab studies salivary glands. We do a lot of immunohistochemistry- and we would like to do more. I am having trouble getting positive beta-galactosidase staining in the salivary glands- however I do get positive staining in other tissues from the same animals. Is this a fixation problem or should I use a different type of anitgen retrieval on salivary glands? Any suggestions would be greatly appreciated. Tara --------------------------------- Have a burning question? Go to Yahoo! Answers and get answers from real people who know. From godsgalnow <@t> aol.com Thu Jan 11 12:17:02 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jan 11 12:17:20 2007 Subject: [Histonet] fish Message-ID: <8C903C3D350D4EF-12A0-490D@FWM-D37.sysops.aol.com> Does anybody know what the regs are for FISH? Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From mary.gessford <@t> spcorp.com Thu Jan 11 12:27:03 2007 From: mary.gessford <@t> spcorp.com (Gessford, Mary) Date: Thu Jan 11 12:27:10 2007 Subject: [Histonet] Bone adhesion problems after HIER Message-ID: Patrick, What type of antibody are you trying get results from? Mary Gessford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leung, Patrick Sent: Thursday, January 11, 2007 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone adhesion problems after HIER Hi, I am having problems with cortical bone detaching from the slide during the antigen retrieval step. The samples I am staining are paraffin embedded mouse tibia. I am currently using Superfrost+ slides. The AR is with citrate buffer (pH6.1) from Dako performed in a waterbath for 15 minutes. The slides are baked overnight at 59C prior to deparaffinizing. I have tried using a steamer and microwave, however I still ended up with the same results. In addition Superfrost GOLD slides did not help in keeping the bone on. I found that performing HIER at 70C for three hrs as suggested in the archives to be ineffective in exposing my antigen (although the bone did stay attached). Do you have any suggestions? Thanks, Patrick ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From anh2006 <@t> med.cornell.edu Thu Jan 11 12:45:05 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Jan 11 12:45:21 2007 Subject: [Histonet] Bone adhesion problems after HIER In-Reply-To: References: Message-ID: Hi Patrick, This is a major problem in the land of mouse bone histology. The only suggestion is to do as little HIER as humanly possible to get the results you need. I have cut mine back to 10 minutes. Still get beautiful immuno with minimal detachment. -- Andrea > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leung, >Patrick >Sent: Thursday, January 11, 2007 12:18 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Bone adhesion problems after HIER > > >Hi, > >I am having problems with cortical bone detaching from the slide during >the antigen retrieval step. The samples I am staining are paraffin >embedded mouse tibia. > >I am currently using Superfrost+ slides. The AR is with citrate buffer >(pH6.1) from Dako performed in a waterbath for 15 minutes. The slides >are baked overnight at 59C prior to deparaffinizing. > >I have tried using a steamer and microwave, however I still ended up >with the same results. In addition Superfrost GOLD slides did not help >in keeping the bone on. I found that performing HIER at 70C for three >hrs as suggested in the archives to be ineffective in exposing my >antigen (although the bone did stay attached). > >Do you have any suggestions? > >Thanks, >Patrick > > > > -- From Jason.Wiese <@t> va.gov Thu Jan 11 12:51:09 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Thu Jan 11 12:51:20 2007 Subject: [Histonet] diastase digestion In-Reply-To: <598233.48959.qm@web31309.mail.mud.yahoo.com> References: <598233.48959.qm@web31309.mail.mud.yahoo.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E1272F@VHAV20MSGA3.v20.med.va.gov> I am not sure why this message was forwarded to the histonet, as I thought it was a personal discussion between two people, and I asked for it not to be repeated. However, since it was... I suppose I should post what my response was... for ... "true clarification". Holly Cow! That goes to show how skewed our views can be of any situation when we depend on word of mouth. Roy Yih is who I met with, and I think I had David Tacha in my head just from hearing the name so many times. Please do not take insult for my ignorance! Both for my confusing of names as well as my lack of knowledge on the race card. I should have learned to keep my mouth shut until I know what I am talking about by now, but... well, that's why I am apologizing. In the week I was in Phoenix I met about a thousand people, and I interviewed with six different companies. It is not that I want out of here, just that I wanted to see what was out there. Whew... for all the people who tell me how smart I am, I feel pretty dumb right now! Also I was speaking out of context when I mentioned whose money started the company. In fact we got quite in depth with how much work has went into the company, and no one was trying to take credit. It was indeed a team effort. I really enjoyed everyone I met at Biocare, and I am sure they have not forgotten what brought them to where they are. I am humbled by your experience and knowledge of my career field. The last thing I want to do is make waves. Please know I am just making conversation, and nothing I have said needs to be repeated. I must get back to work, but thank you for the clarification. At least I can still type with my foot in my mouth. :) Jason -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, January 10, 2007 11:43 PM To: histonet@lists.utsouthwestern.edu Subject: Fwd: RE: [Histonet] diastase digestion --- Akemi Allison-Tacha wrote: > Date: Wed, 10 Jan 2007 16:41:26 -0800 (PST) > From: Akemi Allison-Tacha > Subject: RE: [Histonet] diastase digestion > To: "Wiese, Jason VHAROS" > CC: dave@biocare.net > > Hi Jason, > > Just a heads-up & true clarification. Murray > started > a couple of years ago as a salesman and was not on > the > foundation team. > > Roy Yih is the President & CEO of Biocare. Roy's > father is Chairman of the board. Roy is the person > who now has the long hair. They are chinese not > japanese. The executive VP & CFO is Gene Casagnini. > > These 3 men's money started the company. > > The VP & Director of IHC is Dr. David Tacha. I used > to be the Director of Histology & TMA until 24/7 > took > it's toll and left in 2005. David developed all the > IHC products through his genious, as well as several > instruments. I developed all the H&E and SS > products. > R&D started in 1997 at the old Walnut Creek office > with just a handfull of people on the team. I > started > in 1998. We were lucky to make $5,000 in sales a > month. It took a team to make Biocare what it is > today. I hope they don't forget that. > > Akemi > --- "Wiese, Jason VHAROS" > wrote: > > > Hey thanks for the hello Akemi! > > > > > > > > It is a crazy small world sometimes. Wow... I > love > > the Portland > > Saturday Market! I just met everyone from Biocare > > Medical at the NSH in > > Phoenix. I actually interviewed with David > Tacha... > > well at least I > > thought I did. Perhaps I am confused. They guy I > > met with was Murray, > > but he introduced me to the CEO... I thought his > > name was David. He was > > a younger Asian guy, I believe Japanese, with > long > > hair like a rock > > star or something. He said that he and his father > > had started the > > company with their own money and made it, "what it > > is today." Perhaps I > > met the junior Tacha? Anyway, he was a great guy. > > > Straight forward no > > BS. I would be honored to work for someone with > his > > attitude. He > > offered me a job on the spot, but I am reluctant > to > > leave "god's > > country" to move to crowded California... not to > > mention a pay cut to > > start. I would have to give up my 5 bedroom > > farmhouse on acres of land > > to afford a studio apartment. Not now... maybe > not > > ever... > > > > > > > > Anyway, after returning from Phoenix, I decided to > > stay in Oregon. I > > have lived all over, and I have yet to find a > place > > I feel more at home. > > I truly love it here, and I have turned down jobs > > that would pay me > > twice as much, just so I can stay where I am. I > > take my PA medical > > board exam February 27th, and I am excited about > > that. The only problem > > is that in order to reap the benefits I may have > to > > move. Oh well, I > > guess they can never take the PA certification > away > > once I have it. > > Maybe some day in the future I will find the > perfect > > HT/PA job. Till > > then... I'll be right here. :-) > > > > > > > > I mean absolutely no disrespect by the term "big > > dog", it's just > > something I here quite a bit. It is actually a > > compliment. I try to > > learn something from everyone. There are people > who > > have been in this > > business for decades, and they have tons of valid > > information. However, > > people entering school right now are learning the > > newest technology, and > > they are learning from the mistakes of those who > > came before. As you > > said, there is something to be learned from > > everyone. By the way, I > > have often taken a break in the middle of an > autopsy > > to have lunch, and > > then went back to it. I think spit is about the > > nicest thing we > > encounter in our professions. > > > > > > > > Well, I better get back to work. Hope you have an > > awesome day!!!! > > > > > > > > Jason > > > > > > > > ________________________________ > > > > From: Akemi Allison-Tacha > > [mailto:akemiat3377@yahoo.com] > > Sent: Wednesday, January 10, 2007 3:02 PM > > To: Wiese, Jason VHAROS > > Subject: RE: [Histonet] diastase digestion > > > > > > > > Hi Jason, > > > > > > > > Good to hear your feed back. Great to hear you > have > > a good sense of > > humor from a fellow Oregonian. Some of the people > > in this field haven't > > had your exposure and most likely never will. > > Everyone has a different > > reason for getting into this crazy profession. > > > > > > > > I miss Oregon, it's God's country!. My ex-husband > > Dr. David Tacha of > > Biocare Medical, were from Oregon. I was one of > the > > founders of the > > Portland Saturday Market. I worked up on Pill > Hill, > > Emanuel Hospital, > > as well as setting-up a private derm lab. I > served > > as President of the > > OR Histology Society in 79 & 82 and brought Lee > > Luna, Dezna Sheehan & > > Jules Elias out as speakers. I don't know if I > > consider myself as a"Big > > Dog" I feel that, we all have something to learn > > from each other and > > sometimes it's the puppy that can teach us a thing > > or two. We need to > > have a mind like a parachute. "OPEN" > > > > > > > > I actually took histomicrotechnic at Tokyo > > University from 1966-68. My > > father was stationed in Japan at that time. I > > worked after classes at > > the base hospital. Viet Nam was going on, so I > was > > exposed to quite a > > bit of gruesome stuff. I never thought I would > end > > up in this field. > > > > > > > > After coming back to the states in 1968, I did my > > residency at > > Binghamton General Hospital in NY. Talk about > > exposure! I assisted in > > autopsies too before and after lunch. > > > > > > > > > > > > Best Wishes, > > > > Akemi > > > > > > > > > > > > "Wiese, Jason VHAROS" wrote: > > > > Dinosaur? I graduated from my HT program in 2000. > I > > am but a > > puppy to > > most of you big dogs. However, In the past 6 > years > > of working as > > a > > bench tech, I have found no better digestion > method > > then > > spitting on the > > slide. As far as "the green stuff after lunch > being > > too much for > > the > > youngsters"... try an autopsy with necrotic bowel > > after lunch, > > or why > > not before lunch. > > > > We work in pathology and some of us think spit is > > gross? Maybe > > now is a > > time to re-evaluate your career choice while you > > are still > > young. I was > > in diapers when Bonnie Proctor taught you this > most > > excellent > > method. I > > used it today. Is there a problem with this as > far > > as CAP is > > concerned? > > I have it in my protocol. Even though we all have > a > > different > > enzyme > > make up, I have yet to see different end results > > from different > > people's > > spit. So, as long as I am not breaking some > > cardinal rule I > > say... > > haccc toooowie... :) > > > > > === message truncated === > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy.troiano <@t> yale.edu Thu Jan 11 13:16:20 2007 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Thu Jan 11 13:16:35 2007 Subject: [Histonet] bone adhering to slides during HIER Message-ID: <5.2.1.1.2.20070111141316.027ca7b8@email.med.yale.edu> We adhere our bones to Hybridization slides Cat. #063 from Scientific Device Laboratory, Des Plaines, IL and don't have trouble losing cortical bone from slides during HIER for both paraffin embedded and plastic (MMA) embedded bones. From lpjones <@t> srhs-pa.org Thu Jan 11 13:24:51 2007 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Thu Jan 11 13:25:04 2007 Subject: [Histonet] Is Processed Tissue Biohazardous? Message-ID: <8E7AD740937B954F947F0DB4467EFEE056E9AB@mail.srhs-pa.org> Hi all. We are running into issues surrounding the mailing of paraffin blocks, and whether or not those who package them need the extra training for shipping biohazardous materials. We have always received blocks from other facilities through the mail without any special treatment, and have sent ours the same way. Our safety officer does not agree with this procedure. We do use Shandon's Glyofixx tissue fixative, not formalin, if that makes a difference in your response. We are awaiting a return call from Shandon at this time, but decided to put this out for the opinion of the experts (you!) as well. This is somewhat related to the age old question of when I nick myself on a dirty blade on which I was cutting Glyofixx fixed paraffin embedded tissue, is it a "signifigant exposure"? Our safety people always left this call up to our Pathologist who assured us it was not. Opinions, comments and expert views please. And thanks as always! From cmiller <@t> physlab.com Thu Jan 11 13:37:16 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Jan 11 13:37:28 2007 Subject: [Histonet] diastase Message-ID: <002401c735b7$e6525070$db01a8c0@plab.local> I wish to thank all of you whom sent me product info. I now have several options, although I will miss the "spit time" .Thanks to those of you who agree that spit is the least gross thing we handle everyday. At least I know its mine!!! Let's get this week over, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne From JWEEMS <@t> sjha.org Thu Jan 11 13:39:56 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jan 11 13:40:25 2007 Subject: [Histonet] diastase Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2F84@sjhaexc02.sjha.org> We once had a pathologist tell us our diastase must be getting old, because the stain had not worked so well. We have fun with that tech for years! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cheri Miller Sent: Thursday, January 11, 2007 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I wish to thank all of you whom sent me product info. I now have several options, although I will miss the "spit time" .Thanks to those of you who agree that spit is the least gross thing we handle everyday. At least I know its mine!!! Let's get this week over, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From dpapalegis <@t> gmail.com Thu Jan 11 14:03:23 2007 From: dpapalegis <@t> gmail.com (Derek Papalegis) Date: Thu Jan 11 14:03:30 2007 Subject: [Histonet] thermo vs microm Message-ID: <73382ce30701111203hea60349o4181ace330eb3eaf@mail.gmail.com> Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek From Jason.Wiese <@t> va.gov Thu Jan 11 14:12:49 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Thu Jan 11 14:13:16 2007 Subject: [Histonet] thermo vs microm In-Reply-To: <73382ce30701111203hea60349o4181ace330eb3eaf@mail.gmail.com> References: <73382ce30701111203hea60349o4181ace330eb3eaf@mail.gmail.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12732@VHAV20MSGA3.v20.med.va.gov> I just bought Thermo's Excelsior processor, Gemini stainer, and the Cryotome FE. I love them all. I have had awesome customer service, and the quality of my slides has improved immensely. The processor has reduced waste by more then 75% as you change solutions based on specific gravity, and not on a set schedule. The processor is very user friendly. The Cryotome FE I have only had a couple of months, but it is great. The cryo bar boosts to -50 in minutes. It also has a fumigation cycle that uses only 3mL of formaldehyde solution. My opinion? Thermo rocks! Jason Wiese, BS, HT(ASCP) Roseburg, OR -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Thursday, January 11, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermo vs microm Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Jan 11 14:47:11 2007 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Thu Jan 11 14:46:31 2007 Subject: [Histonet] fish Message-ID: Roxanne, your question may be too general to get a helpful answer. you can view the CAP checklists at http://www.cap.org/apps/cap.portal?_nfpb=true&_pageLabel=accreditation The AP checklist contains some standards for molecular techniques however there is a separate molecular pathology checklist. I would recommend that you look at both. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> 01/11/07 01:17PM >>> Does anybody know what the regs are for FISH? Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jan 11 15:00:30 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 11 15:01:00 2007 Subject: [Histonet] thermo vs microm In-Reply-To: <73382ce30701111203hea60349o4181ace330eb3eaf@mail.gmail.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F7C@EMAIL.archildrens.org> If I were buying a cryostat I would buy a Leica. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Thursday, January 11, 2007 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermo vs microm Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From timothy.macatee <@t> med.nyu.edu Thu Jan 11 15:14:57 2007 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Thu Jan 11 15:16:48 2007 Subject: [Histonet] fish In-Reply-To: Message-ID: They have to go through training like all other histotechs. On 1/11/07 3:47 PM, "Vinnie Della Speranza" wrote: > Roxanne, > your question may be too general to get a helpful answer. > > you can view the CAP checklists at > http://www.cap.org/apps/cap.portal?_nfpb=true&_pageLabel=accreditation > The AP checklist contains some standards for molecular techniques however > there is a separate molecular pathology checklist. I would recommend that you > look at both. > > Vinnie > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, SC 29425 > Ph: 843-792-6353 > fax: 843-792-8974 > >>>> 01/11/07 01:17PM >>> > Does anybody know what the regs are for FISH? > > Roxanne > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and security > tools, free access to millions of high-quality videos from across the web, > free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From rjbuesa <@t> yahoo.com Thu Jan 11 15:49:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 11 15:49:08 2007 Subject: [Histonet] Bone adhesion problems after HIER In-Reply-To: <4D1A82B94D90CC449EC8395E1DCF0C090C51FB@usctmx1134.merck.com> Message-ID: <145622.69493.qm@web61218.mail.yahoo.com> Patrick: I am somewhat confused about your e-mail, because it is titled "bone adhesion problems" and at the end it seems that you solved that problem, but are having problems with the IHC reaction. 1-for the adhesion: I always used 1 mL of Elmer's glue in a regular 2L water bath and "fished" the sections with superfrost + slides (for both nails and bones). Did not lose sections during HIER. 2-about the failure with IHC I cannot try to help you if I do not know what epitope your are looking for. Ren? J. "Leung, Patrick" wrote: Hi, I am having problems with cortical bone detaching from the slide during the antigen retrieval step. The samples I am staining are paraffin embedded mouse tibia. I am currently using Superfrost+ slides. The AR is with citrate buffer (pH6.1) from Dako performed in a waterbath for 15 minutes. The slides are baked overnight at 59C prior to deparaffinizing. I have tried using a steamer and microwave, however I still ended up with the same results. In addition Superfrost GOLD slides did not help in keeping the bone on. I found that performing HIER at 70C for three hrs as suggested in the archives to be ineffective in exposing my antigen (although the bone did stay attached). Do you have any suggestions? Thanks, Patrick ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From mcauliff <@t> umdnj.edu Thu Jan 11 15:50:59 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jan 11 15:50:44 2007 Subject: [Histonet] vibrating microtome blades In-Reply-To: <7dad1aa6170d9.45a4c4bf@med.cornell.edu> References: <7dad1aa6170d9.45a4c4bf@med.cornell.edu> Message-ID: <45A6B143.1040102@umdnj.edu> I like the Schick Platinum Plus blades as well. The thicker injector-style blades flex less than other types. Geoff William Ares wrote: >My lab recently bought a Leica VT1000S vibrating microtome and as I was unhappy with the saphire blade that the model was offered with, we have been circulating through numerous brands of disposable metal blades. Does anyone have a suggestion regarding disposable blades for cutting fixed and live brain tissue? Thx in advance. > >William Ares >Research Technician >Labratory of Molecular and Developmental Neuroscience >Weill Medical College of Cornell University >Tel: (212) 746 5056 >Email: wia2005@med.cornell.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Thu Jan 11 15:55:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 11 15:55:53 2007 Subject: [Histonet] thermo vs microm In-Reply-To: <73382ce30701111203hea60349o4181ace330eb3eaf@mail.gmail.com> Message-ID: <801835.60510.qm@web61211.mail.yahoo.com> Had I been in your position I would buy a Sakura tissue processor and a Leica cryostat. Ren? J. Derek Papalegis wrote: Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From rjbuesa <@t> yahoo.com Thu Jan 11 15:59:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 11 15:59:53 2007 Subject: [Histonet] Is Processed Tissue Biohazardous? In-Reply-To: <8E7AD740937B954F947F0DB4467EFEE056E9AB@mail.srhs-pa.org> Message-ID: <178076.68046.qm@web61213.mail.yahoo.com> There are 2 separate issues: are processed tissues biohazardous?; and how should they be shipped?: 1- except for blocks from known JKD cases, that should be treated very carefully, FFTP are not biohazardous; and 2-shipment of any human material, fresh or otherwise, should be labelled as "biohazardous". Ren? J. "Jones, Laura" wrote: Hi all. We are running into issues surrounding the mailing of paraffin blocks, and whether or not those who package them need the extra training for shipping biohazardous materials. We have always received blocks from other facilities through the mail without any special treatment, and have sent ours the same way. Our safety officer does not agree with this procedure. We do use Shandon's Glyofixx tissue fixative, not formalin, if that makes a difference in your response. We are awaiting a return call from Shandon at this time, but decided to put this out for the opinion of the experts (you!) as well. This is somewhat related to the age old question of when I nick myself on a dirty blade on which I was cutting Glyofixx fixed paraffin embedded tissue, is it a "signifigant exposure"? Our safety people always left this call up to our Pathologist who assured us it was not. Opinions, comments and expert views please. And thanks as always! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From anh2006 <@t> med.cornell.edu Thu Jan 11 16:02:21 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Jan 11 16:02:37 2007 Subject: [Histonet] Bone adhesion problems after HIER In-Reply-To: <145622.69493.qm@web61218.mail.yahoo.com> References: <145622.69493.qm@web61218.mail.yahoo.com> Message-ID: Hi Rene, I think the IHC problems only arose b/c Patrick dropped the temp of retrieval down to 70 deg C. I can confirm from personal experience that for most antigens it doesn't work. Even 80 deg C (the point at which the sections start to come off Plus slides) is largely ineffective. 95-99 deg C is critical retrieval temp in my experience. I am going to try those slides recommended by Nancy, maybe Patrick can do the same (or your Elmer's method which for me isn't an option as we don't cut our own sections anymore so cannot control those parameters too easily). Best, Andrea >Patrick: > I am somewhat confused about your e-mail, >because it is titled "bone adhesion problems" >and at the end it seems that you solved that >problem, but are having problems with the IHC >reaction. > 1-for the adhesion: I always used 1 mL of >Elmer's glue in a regular 2L water bath and >"fished" the sections with superfrost + slides >(for both nails and bones). Did not lose >sections during HIER. > 2-about the failure with IHC I cannot try to >help you if I do not know what epitope your are >looking for. > Ren? J. > >"Leung, Patrick" wrote: > Hi, > >I am having problems with cortical bone detaching from the slide during >the antigen retrieval step. The samples I am staining are paraffin >embedded mouse tibia. > >I am currently using Superfrost+ slides. The AR is with citrate buffer >(pH6.1) from Dako performed in a waterbath for 15 minutes. The slides >are baked overnight at 59C prior to deparaffinizing. > >I have tried using a steamer and microwave, however I still ended up >with the same results. In addition Superfrost GOLD slides did not help >in keeping the bone on. I found that performing HIER at 70C for three >hrs as suggested in the archives to be ineffective in exposing my >antigen (although the bone did stay attached). > >Do you have any suggestions? > >Thanks, >Patrick > -- From godsgalnow <@t> aol.com Thu Jan 11 16:14:26 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jan 11 16:14:42 2007 Subject: [Histonet] thermo vs microm In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F7C@EMAIL.archildrens.org> Message-ID: <8C903E4FD9FE3A9-5D4-67F4@FWM-D44.sysops.aol.com> Ditto Roxanne -----Original Message----- From: HornHV@archildrens.org To: dpapalegis@gmail.com; histonet@lists.utsouthwestern.edu Sent: Thu, 11 Jan 2007 4:00 PM Subject: RE: [Histonet] thermo vs microm If I were buying a cryostat I would buy a Leica. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Thursday, January 11, 2007 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermo vs microm Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From kmilne <@t> bccancer.bc.ca Thu Jan 11 16:40:06 2007 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Jan 11 16:40:14 2007 Subject: [Histonet] B cells in mouse FFPE tumor - retry Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780240D290@srvex03.phsabc.ehcnet.ca> Hi everyone, not sure if this worked last time so I'll try again... I have to look for an antibody to detect B cells in mouse formalin-fixed paraffin embedded tissue and I'm not looking forward to it given the troubles I've had with some other CD markers. Is there anyone that could recommend a reliable antibody, preferrably not a mouse monoclonal as I'd like to avoid the extra cost of bringing in a MOM kit. I was thinking of using CD19 but if anyone can suggest another reliable marker that isn't only present on subsets of cells it would be much appreciated. Thanks, Katy Milne Deeley Research Centre From pardeepjasra <@t> hotmail.com Thu Jan 11 18:02:02 2007 From: pardeepjasra <@t> hotmail.com (Dr.Pardeep jasra) Date: Thu Jan 11 18:34:48 2007 Subject: [Histonet] unsubscribe Message-ID: Dear sir/madam Please remove my name from the mailing list Thanks Pardeep _________________________________________________________________ Be one of the first to try Windows Live Mail. http://ideas.live.com/programpage.aspx?versionId=5d21c51a-b161-4314-9b0e-4911fb2b2e6d From Traczyk7 <@t> aol.com Thu Jan 11 18:34:51 2007 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Thu Jan 11 18:35:09 2007 Subject: [Histonet] Thermo vs. everyone else Message-ID: Thought I would share this. I need to acquire an accessory to use for one of my upcoming demonstrations. Easy enough, get the part number and call Fisher. Our account is already set up, ordered stuff w/o a problems in the past, discount established. Here's where it gets good... I was told by a Fisher CSR that since it was a Thermo part, Fisher could only buy it "at list" from Thermo. It would then be marked up by Fisher before it was sold to me. If I buy direct from Thermo I can get it at list. I thought they were all one big happy family now! Just curious, what's the rumor mill saying about how the product lines will shake out? Happy Friday. Dorothy From anh2006 <@t> med.cornell.edu Thu Jan 11 18:42:09 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Thu Jan 11 18:42:22 2007 Subject: [Histonet] B cells in mouse FFPE tumor - retry In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC065780240D290@srvex03.phsabc.ehcnet.ca> References: <07979E76B0869D4E8C9FE4AA9FC065780240D290@srvex03.phsabc.ehcnet.ca> Message-ID: Rat anti-mouse B220 from BD Pharmingen works like a charm in frozens and PARAFFIN. I promise :) Good luck, Andrea At 2:40 PM -0800 1/11/07, Milne, Katy wrote: > Hi everyone, not sure if this worked last time so I'll try again... > >I have to look for an antibody to detect B cells in mouse formalin-fixed >paraffin embedded tissue and I'm not looking forward to it given the >troubles I've had with some other CD markers. > >Is there anyone that could recommend a reliable antibody, preferrably >not a mouse monoclonal as I'd like to avoid the extra cost of bringing >in a MOM kit. I was thinking of using CD19 but if anyone can suggest >another reliable marker that isn't only present on subsets of cells it >would be much appreciated. > >Thanks, >Katy Milne >Deeley Research Centre -- From jqb7 <@t> cdc.gov Thu Jan 11 19:54:49 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jan 11 19:54:58 2007 Subject: [Histonet] thermo vs microm References: <73382ce30701111203hea60349o4181ace330eb3eaf@mail.gmail.com> Message-ID: I have had great success with Microm microtomes. Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis Sent: Thu 1/11/2007 3:03 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] thermo vs microm Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BJDewe <@t> aol.com Thu Jan 11 20:00:35 2007 From: BJDewe <@t> aol.com (BJDewe@aol.com) Date: Thu Jan 11 20:00:52 2007 Subject: [Histonet] thermo vs microm Message-ID: <3bf.fe1884d.32d845c3@aol.com> It depends on the product since now Thermo, Fisher and Richard Allen are all under one umbrella. I too have always liked the Microm microtomes especially the HM 355S! But don't get sold into their slide waterbath contraption!! Not a good thing ;-) Always, Loralei Dewe Histotechnologist UC Davis, Davis CA From fawaz.zouabi <@t> email.cs.nsw.gov.au Thu Jan 11 20:23:03 2007 From: fawaz.zouabi <@t> email.cs.nsw.gov.au (fawaz) Date: Thu Jan 11 20:27:00 2007 Subject: [Histonet] (no subject) Message-ID: <002401c735f0$96885a10$277b4c98@dofm.cs.nsw.gov.au> I have used microm microtomes for 5 year now. Never complained of any problem with the desing and cutting and it can handle lots of hard work. FAWAZ ZOUABI Histo-Technologist DOFM Glebe,NSW fawaz.zouabi@email.cs.nsw.gov.au From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jan 12 01:59:54 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jan 12 02:00:03 2007 Subject: [Histonet] Is Processed Tissue Biohazardous? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247106@wahtntex2.waht.swest.nhs.uk> Blocks that have been formalin fixed and processed can still retain TB in the calcified areas that has been untouched by chemicals, or is that an 'urban myth'? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com Everybody needs beauty as well as bread, places to play in and pray in, where nature may heal and give strength to body and soul. --John Muir Aint't that the truth!!!!! This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ree3 <@t> leicester.ac.uk Fri Jan 12 03:30:26 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Jan 12 03:30:40 2007 Subject: [Histonet] fish In-Reply-To: <8C903C3D350D4EF-12A0-490D@FWM-D37.sysops.aol.com> Message-ID: Best served with chips, and if feeling the need for healthy eating, mushy peas. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 11 January 2007 18:17 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish Does anybody know what the regs are for FISH? Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Thu Jan 11 19:11:21 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Jan 12 05:27:09 2007 Subject: [Histonet] Updated list of Histology opportunites near you - Should I call you to discuss? Message-ID: Hi - Histonetters - I have both permanent and full time (permanent) jobs for HistoTechs throughout the US. Do you know that I can speak to about these HistoTech jobs ? Should I call you to discuss? These permanent and temporary HistoTech jobs are being filled quickly, don't miss out on your dream job- call today! Histology Temporary Assignments (Updated Jan 11 2007) ------------------------------------------------------- 1. California (Palm Springs) - Hot-Hot- Histotech with ISH experience- 13 weeks- Needs to be filled by 1/19/07 2. Hot ! - Southern Texas, Great Location - Histotech-Must have IHC experience - Call Today- Needs To Be Filled By 1/15/07 3. Texas (Southwest) - 13 weeks -Histo Tech- Filled Permanent Histology Jobs: (Updated Jan 11 2007) ------------------------------------------------------- 01. Central Virginia- Histotech- perm- BrandNew 02. Southern California- Histotech- perm- BrandNew 03. Southern Idaho- Histotech - perm BrandNew- Filled 04. Central Florida - Histotech with some MOHS experience - perm - BrandNew Hot 05. Southern Alabama - Histotech - basic histology with opportunity to learn new and non-routine Histology -perm- BrandNew- Filled 06. Southeast South Dakota- Histotech - Must have ties to South Dakota- perm- BrandNew-Filled 07. Southwest Florida- Histotech Supervisor- perm - BrandNew (Need Florida License)-Filled 08. Southwest Texas- Bench Histotech- perm - BrandNew-Filled 09. Hot! Eastern, Massachusetts Seeking Histotechs of all experience levels, Great Pay, Location And Benefits- Call Today - 15% Pay Raise Guaranteed! 10. NEWJOB! Southeast Florida- Histotech - perm - (Need Florida License) 11. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 12. Northern New Jersey - Histotech - perm-Filled 13. Eastern Mass - Histotech - one Senior Histotech, One not so Senior Histotech- Filled 14. Eastern Mass - Histotech - Histotech -perm- Filled 15. Massachusetts (North of Boston) - perm - Bench Histotech- Filled 16. Central Florida -perm- Histotech (Need Florida License) 17. Southeast Florida - Treasure Coast - perm - Histotech (Need Florida License)- Filled 18. Southeast Florida - perm - Histotech (Need Florida License)-Filled 19. Florida, West Coast - both temp & perm openings- Bench Histotech -Very-Hot 20. New York ( Syracuse area) - Bench Histotech- perm 21. New York City (Long Island) - Bench- perm 22. Central Florida - Bench Histotech- perm 23. Las Vegas - Bench Histotech- perm 24. Wisconsin- Histology Supervisor- BrandNew -Hot Hot Hot 25. Central-Illinois-Bench-Dermpath- BrandNew -Hot 26. Northern California- Bench- Routine Histology- BrandNew -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From yjones2 <@t> csmlab.com Fri Jan 12 06:37:25 2007 From: yjones2 <@t> csmlab.com (Yvonne Jones) Date: Fri Jan 12 06:39:33 2007 Subject: [Histonet] Job Opening Message-ID: <45A73AB50200004F000004F4@GWGATE1.ahm.com> We are currently looking for 2 part-time histotechs. Shifts are as follows: 1. Mon-Fri, 2am to 7am; some weekends as needed 2. Tues-Fri 1:30am to 6am, some weekends as needed Both techs will be required to cut biopsies and routine specimens. Knowledge of special stains and/or IHC a plus!! Please direct all inquiries to... Cytology and Histology Services of Maryland 13900 Laurel Lakes Ave Laurel, MD 20707 Ph:301-206-2555 x48 (ask for Simone or Yvonne) Fx:301-206-2595----------------------------------------- From mhkatie <@t> aol.com Fri Jan 12 07:13:32 2007 From: mhkatie <@t> aol.com (mhkatie@aol.com) Date: Fri Jan 12 07:13:48 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <200701110243.dc45a5eaaee0@rly-mb03.mail.aol.com> References: <200701110243.dc45a5eaaee0@rly-mb03.mail.aol.com> Message-ID: <8C90462981F33FA-7CC-2FA@FWM-D35.sysops.aol.com> Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From c.m.vanderloos <@t> amc.uva.nl Fri Jan 12 08:35:25 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Jan 12 08:35:55 2007 Subject: [Histonet] RE: fluorescent mouse to mouse Message-ID: <45d052458239.45823945d052@amc.uva.nl> Dear Tara, A Zenon kit ([1]www.invitrogen.com and search for "zenon") is a good suggestion. A fluorescein (or Alexa) conjugated anti-mouse Fab fragment is in vitro bound to your primary. After completing the "conjugation" incubate your section with the antibody complex. Be aware you have to check your mouse isotype before purchasing. The protocol for labeling is not included with the kit we purchased (!) and appeared also very difficult to find at their website (ooops): use 5 ul of labeling reagent per ug of mouse IgG (5 min, RT), block with 5 ul of blocking reagent (5 min, RT); then prepare dilution(s). I would not advise an overnight 4C incubation; just 30-60 min at RT. Lots of success! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 11 Jan 2007 10:12:25 -0800 (PST) From: Tara Bullard Subject: [Histonet] fluorescent mouse to mouse To: histonet@lists.utsouthwestern.edu Hello All, I am new to the list. I have 2 separate questions which I will spost separately. The first question- is there a good kit for mouse to mouse fluorescent immunohistochemistry? Thank you, Tara References 1. http://www.invitrogen.com/ From akemiat3377 <@t> yahoo.com Fri Jan 12 08:43:42 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jan 12 08:43:51 2007 Subject: [Histonet] Microm Message-ID: <835966.61234.qm@web31315.mail.mud.yahoo.com> Diddo with info below. The water bath attachment needs a lot of work. I used the microtome for TMA & recuts and loved it! Although, I'd go with the X,Y,Z attachment too, especially if you are doing recuts on precious tissue. Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com It depends on the product since now Thermo, Fisher and Richard Allen are all under one umbrella. I too have always liked the Microm microtomes especially the HM 355S! But don't get sold into their slide waterbath contraption!! Not a good thing ;-) Always, Loralei Dewe Histotechnologist UC Davis, Davis CA From akemiat3377 <@t> yahoo.com Fri Jan 12 08:43:40 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jan 12 08:43:54 2007 Subject: [Histonet] Microm Message-ID: <229504.56436.qm@web31309.mail.mud.yahoo.com> Diddo with info below. The water bath attachment needs a lot of work. I used the microtome for TMA & recuts and loved it! Although, I'd go with the X,Y,Z attachment too, especially if you are doing recuts on precious tissue. Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com It depends on the product since now Thermo, Fisher and Richard Allen are all under one umbrella. I too have always liked the Microm microtomes especially the HM 355S! But don't get sold into their slide waterbath contraption!! Not a good thing ;-) Always, Loralei Dewe Histotechnologist UC Davis, Davis CA From rjbuesa <@t> yahoo.com Fri Jan 12 08:58:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 12 08:58:28 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <8C90462981F33FA-7CC-2FA@FWM-D35.sysops.aol.com> Message-ID: <20070112145820.89118.qmail@web61214.mail.yahoo.com> Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From JEllin <@t> yumaregional.org Fri Jan 12 09:24:10 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Jan 12 09:24:38 2007 Subject: [Histonet] Re: Block indentifiers Message-ID: <29BE166A2CF48D459853F8EC57CD37E87E9570@EXCHANGECLUSTER.yumaregional.local> We are currently going to be putting 2D barcodes on our cassettes to satisfy this requirment, the problem comes when your PIS is down and you are hand writing cassettes. But if the last name is the only thing that you put on this block how is it going to give an Identification. You will need the entire name on there to really have any type of use to find out information. Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 7:58 AM To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Block indentifiers Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From bwhitaker <@t> brownpathology.com Fri Jan 12 09:29:24 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Jan 12 09:24:41 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <20070112145820.89118.qmail@web61214.mail.yahoo.com> Message-ID: <001c01c7365e$70c20e20$3601a8c0@brownpathology.net> I would disagree with this in principle and practice. The last name only doesn't provide any personal protected information. If you have only the last name and the surgical number, you would have to have access to additional (protected) information in order to associate the last name only and a surgical case number with an individual. (At least that is my interpretation of the HIPPA rules and regulations.) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 8:58 AM To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Block indentifiers Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================ == ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Fri Jan 12 09:41:19 2007 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Jan 12 09:41:37 2007 Subject: [Histonet] Unsubscribe Message-ID: Sorry folks I'm changing jobs and will be off line for a while. Juan C. Gutierrez, HT(ASCP) From Nancy.Temple <@t> ssfhs.org Fri Jan 12 09:59:06 2007 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Fri Jan 12 09:59:20 2007 Subject: [Histonet] thermo vs microm Message-ID: We have 2 of the Microm cryostats with vacutomes, and our pathologists really like them. We also have 1 Microm microtome (HM315) and the tech that uses it absolutely loves it. It does cut much better than our other microtomes which are Reichert-Jung 2030, and a Leica RM2135. We would definitely purchase Micron cryostats or microtomes again. Nancy Temple HT(ASCP) Supervisor Histology/Cytology St. Francis Hospital Indianapolis, IN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Thursday, January 11, 2007 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermo vs microm Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barrickstacey <@t> yahoo.com Fri Jan 12 09:59:49 2007 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Fri Jan 12 10:00:00 2007 Subject: [Histonet] Microm In-Reply-To: <229504.56436.qm@web31309.mail.mud.yahoo.com> Message-ID: <825999.20709.qm@web54314.mail.yahoo.com> I agree. we also had LOTS of problems with the slide and waterbath. It was horrible. You have to keep replacing the material on the slide, the water does not flow evenly, etc. Too many problems. Akemi Allison-Tacha wrote: Diddo with info below. The water bath attachment needs a lot of work. I used the microtome for TMA & recuts and loved it! Although, I'd go with the X,Y,Z attachment too, especially if you are doing recuts on precious tissue. Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com It depends on the product since now Thermo, Fisher and Richard Allen are all under one umbrella. I too have always liked the Microm microtomes especially the HM 355S! But don't get sold into their slide waterbath contraption!! Not a good thing ;-) Always, Loralei Dewe Histotechnologist UC Davis, Davis CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From doug <@t> ppspath.com Fri Jan 12 10:10:24 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Jan 12 10:07:57 2007 Subject: [Histonet] Joint guideline on testing for HER2 status in invasive breast cancer Message-ID: Has anyone had a chance to decipher this new guideline for HER2 testing? I think that overall it is a good idea but I have an issue with appendix E. It says that "Fixation times for needle biopsies have not been addressed." Why put out a guideline when the initial HER2 testing is done on the needle biopsy? It also says "Breast specimens, after appropriate gross inspection and designation of margins, should be promptly sliced at 5- to 10-mm intervals and fixed in formalin (unsliced samples should not be fixed). The interval between tissue acquisition and fixation of breast specimens should be as short as possible." Am I off on this one by thinking that "unsliced samples should not be fixed" means that the breast should be delivered fresh? Does this mean that the sample that is not placed in cassettes is not to be fixed? What do you do with the unsliced/unfixed sample then? I need some coffee! Someone straighten me out. http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Heather.D.Renko <@t> osfhealthcare.org Tue Jan 9 13:26:32 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Jan 12 10:42:36 2007 Subject: [Histonet] block Identifiers Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777528D@pmc-rfd-mx01.intranet.osfnet.org> In our lab we hand label all of our cassettes with the Surgical number only-currently. We are exploring the idea of adding another identifier to our cassettes. Can you let me know what all of you are doing and your thoughts. Thank you in advance. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From LGaliotto <@t> nch.org Fri Jan 12 10:51:14 2007 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Fri Jan 12 10:51:22 2007 Subject: [Histonet] Re: Block indentifiers Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B1626C@NCH01EX02.nch.org> Hello Histonetter's I am curious to those that are not using the patient's name as a second identifier. What are you using, since JCAHO is requiring a second identifier? Laura -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 8:58 AM To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Block indentifiers Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From tkngflght <@t> yahoo.com Fri Jan 12 10:57:46 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri Jan 12 10:57:52 2007 Subject: Her2 article in CAP Today RE: [Histonet] Joint guideline on testing for HER2 status in invasivebreast cancer Message-ID: <003601c7366a$c91ef130$6401a8c0@FSDESKTOP> Hey Doug-- See if anyone in your lab subscribes to the publication "CAP Today". The December 06 edition has a (long) article that explains a lot of the 'why' behind the regulations and helps interpret what is being said. There are short interjections by people such as Dr. Allen Gown. This edition also sports a shorter article on the changed to streamline the 'surprise inspection' CAP that started this past summer. Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, January 12, 2007 10:10 AM To: 'histonet' Subject: [Histonet] Joint guideline on testing for HER2 status in invasivebreast cancer Has anyone had a chance to decipher this new guideline for HER2 testing? I think that overall it is a good idea but I have an issue with appendix E. It says that "Fixation times for needle biopsies have not been addressed." Why put out a guideline when the initial HER2 testing is done on the needle biopsy? It also says "Breast specimens, after appropriate gross inspection and designation of margins, should be promptly sliced at 5- to 10-mm intervals and fixed in formalin (unsliced samples should not be fixed). The interval between tissue acquisition and fixation of breast specimens should be as short as possible." Am I off on this one by thinking that "unsliced samples should not be fixed" means that the breast should be delivered fresh? Does this mean that the sample that is not placed in cassettes is not to be fixed? What do you do with the unsliced/unfixed sample then? I need some coffee! Someone straighten me out. http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Jan 12 10:59:38 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri Jan 12 10:59:53 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC304B1626C@NCH01EX02.nch.org> References: <270614B321ACB44D8C1D91F4F921FDC304B1626C@NCH01EX02.nch.org> Message-ID: <003701c7366b$0c344060$6401a8c0@FSDESKTOP> On confidentiality policies-- These policies are to protect patient information from people who do not have the right to see it. Wouldn't anyone handling blocks have not just the right, but the need to validate the ID of the block to the associated patient report? Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Friday, January 12, 2007 10:51 AM To: Rene J Buesa; mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers Hello Histonetter's I am curious to those that are not using the patient's name as a second identifier. What are you using, since JCAHO is requiring a second identifier? Laura -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 8:58 AM To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Block indentifiers Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================ == ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jan 12 11:23:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 12 11:23:29 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <003701c7366b$0c344060$6401a8c0@FSDESKTOP> Message-ID: <20070112172320.19925.qmail@web61224.mail.yahoo.com> Consider the following: the blocks are going to be sent out for a consult: how many people unrelated to the lab will be able to read the name in the block? Do those unrelated people have the "right" to read that name? Even those with that "right" could find out about a certain name and make some commentary to another person without the "right" to know. There is a big diference between the "right" to know and the "need to know". A second log number, a date or a bar code are better and anonymous second identifiers. I would not like my name to be written in a cassette. Ren? J. Cheryl Kerry wrote: On confidentiality policies-- These policies are to protect patient information from people who do not have the right to see it. Wouldn't anyone handling blocks have not just the right, but the need to validate the ID of the block to the associated patient report? Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Friday, January 12, 2007 10:51 AM To: Rene J Buesa; mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers Hello Histonetter's I am curious to those that are not using the patient's name as a second identifier. What are you using, since JCAHO is requiring a second identifier? Laura -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 8:58 AM To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Block indentifiers Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================ == ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From victor <@t> pathology.washington.edu Fri Jan 12 11:35:35 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Jan 12 11:35:49 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <20070112172320.19925.qmail@web61224.mail.yahoo.com> References: <20070112172320.19925.qmail@web61224.mail.yahoo.com> Message-ID: <45A7C6E7.6020005@pathology.washington.edu> Depending upon the situation of the consult, a copy of the report is probably accompanying the block or slide, so what harm is the name? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: > Consider the following: > the blocks are going to be sent out for a consult: how many people unrelated to the lab will be able to read the name in the block? > Do those unrelated people have the "right" to read that name? Even those with that "right" could find out about a certain name and make some commentary to another person without the "right" to know. > There is a big diference between the "right" to know and the "need to know". > A second log number, a date or a bar code are better and anonymous second identifiers. > I would not like my name to be written in a cassette. > Ren? J. > > Cheryl Kerry wrote: > On confidentiality policies-- > > These policies are to protect patient information from people who do not > have the right to see it. Wouldn't anyone handling blocks have not just the > right, but the need to validate the ID of the block to the associated > patient report? > > Cheryl > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, > Laura > Sent: Friday, January 12, 2007 10:51 AM > To: Rene J Buesa; mhkatie@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Block indentifiers > > Hello Histonetter's > I am curious to those that are not using the patient's name as a second > identifier. What are you using, since JCAHO is requiring a second > identifier? > Laura > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Friday, January 12, 2007 8:58 AM > To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: Block indentifiers > > > Word of caution about patient's name in cassettes or slides: > > check you "confidentiality policies" before doing that because the > patient's name will be available to any with access to either the cassette > or the slide. > We never allowed that type of "second" identification. > Ren? J. > > mhkatie@aol.com wrote: > > > Our facility puts the patients last name on the cassettes as the second > identifier. > > Ann Lynde > Medicine Hat Diagnostic Laboratory > Medicine Hat, Alberta > 403-527-3989 > Message: 6 > Date: Wed, 10 Jan 2007 13:50:02 -0600 > From: "Renko, Heather D." > Subject: [Histonet] Block identifiers > To: Histonet@lists.utsouthwestern.edu > Message-ID: > <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> > > Content-Type: text/plain; charset=iso-8859-1 > > I would like some input on handwritten identifiers on blocks/cassettes. Our > facility currently puts the surgical number only on our blocks and is > considering the idea of a second identifier. > > Heather Renko, Histology Coordinator > OSF Saint Anthony Medical Center > 5666 East State Street > Rockford, Illinois 61108 > 815-395-5410 > heather.renko@osfhealthcare.org > > > ============================================================================ > == > The information in this message is confidential and may be legally > privileged. > Access to this message by anyone other than the addressee is not authorized. > If > you are not the intended recipient, or an agent of the intended recipient, > any > disclosure, copying, or distribution of the message or any action or > omission > taken by you in reliance on it, is prohibited and may be unlawful. If you > have > received this message in error, please contact the sender immediately and > permanently delete the original e-mail, attachment(s), and any copies. > ============================================================================ > == > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and security > tools, free access to millions of high-quality videos from across the web, > free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ******************* PLEASE NOTE ******************* > This E-Mail/telefax message and any documents accompanying this transmission > may contain information that is privileged, confidential, and/or exempt from > disclosure under applicable law and is intended solely for the addressee(s) > named above. If you are not the intended addressee/recipient, you are > hereby notified that any use of, disclosure, copying, distribution, or > reliance on the contents of this E-Mail/telefax information is strictly > prohibited and may result in legal action against you. Please reply to the > sender advising of the error in transmission and immediately delete/destroy > the message and any accompanying documents. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > --------------------------------- > Now that's room service! Choose from over 150,000 hotels > in 45,000 destinations on Yahoo! Travel to find your fit. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From asmith <@t> mail.barry.edu Fri Jan 12 11:39:24 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Jan 12 11:39:36 2007 Subject: [Histonet] block identifiers Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4026@exchsrv01.barrynet.barry.edu> It is rather ironic that federal law requires hospitals to risk killing a patient in a mistaken identity incident in order to protect his privacy. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From proctoth <@t> ohsu.edu Fri Jan 12 11:52:31 2007 From: proctoth <@t> ohsu.edu (Thomas Proctor) Date: Fri Jan 12 11:52:52 2007 Subject: [Histonet] Luxol Fast Blue Message-ID: I'm trying to stain mouse spinal cord sections, (7 um), with Luxol Fast Blue and periodic acid/Schiff and hematoxylin. In the literature I've been reading, there are some great examples of a nice pink to red stain of the grey matter, and a nice solid blue stain of the white matter. Mine look not so good, (purple), and I've been struggling for a while with it. I've tried so many adjustments to the protocol that it would be too long to list here. But I think the heart of my problem is with LFB, differentiating the grey matter completely with out fading the blue-stained white matter. Somebody please tell me the secret! I've experimented with the LFB alone, and believe that differentiating in lithium carbonate, with or with out 70% EtOH, should clear the grey matter of the stain, and leave the white matter blue or turquoise. But it only clears it somewhat, and any further attempt to differentiate also quickly fades the white matter. Any help is greatly appreciated. Thomas From dellav <@t> musc.edu Fri Jan 12 11:42:10 2007 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Jan 12 11:56:48 2007 Subject: [Histonet] Joint guideline on testing for HER2 status in invasivebreast cancer Message-ID: Douglas, I have not read through the guideline which is quite a lengthy document but I'll comment on your questions with my best guess. others who've thoroughly studied the guideline are free to disagree. my understanding and what I believe is the basis for the creation of this guideline is that previous lack of correlation of Her 2 results when comparing needle core results with lumpectomy. Our needle cores arrive in formalin and given their relatively small size, concern about adequate fixation of needle cores is much less likely to arise than the lumpectomy samples, which arrive unfixed and may sit before being grossed in and placed into formalin. This variation is largely the root cause of a failure of results to correlate between needle cores and the lumpectomy specimen, in my opinion. In addition, it might be difficult for most labs to determine with any degree of accuracy exactly how much fixation time a needle core received since the lab can't control when the sample is introduced into formalin. not so with lumpectomy samples. I believe the standard intends that lumpectomy specimens arrive at Pathology fresh and that these are to be cut down and placed in fixative with minimum delay. in addition, minimum and maximum fixation times are proposed (no less than six hours in formalin, no more than 48). if you typically start your processor on Friday evening with a delay start with tissues sitting in formalin, this upper fixation limit may be an issue for you. in regard to your question " Does this mean that the sample that is not placed in cassettes is not to be fixed?" the answer is NO. they are just insisting that specimens be cut down to reasonable thicknesses before immersion in formalin. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Douglas D Deltour" 01/12/07 11:10AM >>> Has anyone had a chance to decipher this new guideline for HER2 testing? I think that overall it is a good idea but I have an issue with appendix E. It says that "Fixation times for needle biopsies have not been addressed." Why put out a guideline when the initial HER2 testing is done on the needle biopsy? It also says "Breast specimens, after appropriate gross inspection and designation of margins, should be promptly sliced at 5- to 10-mm intervals and fixed in formalin (unsliced samples should not be fixed). The interval between tissue acquisition and fixation of breast specimens should be as short as possible." Am I off on this one by thinking that "unsliced samples should not be fixed" means that the breast should be delivered fresh? Does this mean that the sample that is not placed in cassettes is not to be fixed? What do you do with the unsliced/unfixed sample then? I need some coffee! Someone straighten me out. http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbradshaw <@t> lcpath.com Fri Jan 12 12:03:09 2007 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri Jan 12 12:03:18 2007 Subject: [Histonet] RE: block identifiers In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E4026@exchsrv01.barrynet.barry.edu> Message-ID: <22fb4f5cba558240a6f9fc6f29c0aa12@mail2.lcpath.com> Hooray! Very well put! Kari L. Bradshaw Laboratory Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360-425-5620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, January 12, 2007 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block identifiers It is rather ironic that federal law requires hospitals to risk killing a patient in a mistaken identity incident in order to protect his privacy. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Rush <@t> sjmcmn.org Fri Jan 12 12:22:54 2007 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Fri Jan 12 12:23:05 2007 Subject: [Histonet] Cassette Identifier Message-ID: <2337362E8548BE4C85EE5DD5D526B085744B76@sjw3smail2.SJMCMN.ORG> Currently we have only the surgical number on our cassettes. I really don't think that there is a problem with having a patient name, also, since cases sent out are, in our institution, for continuation of care. Since this thread has come up it has made me think that a second identifier would probably be a good thing. I'd be very interested in knowing what labeling method folks use, if an instrument, which one?is it interfaced to an AP computer system?, etc. We have Cerner Millennium software and are checking with them to see what is available. Thank you all so much for your help. Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 DISCLAIMER: This email and any files transmitted with it are confidential and intended solely for the use of histonet@lists.utsouthwestern.edu. If you have received this email in error please notify Rush, Joyce and destroy/delete the message. Please note that any views or opinions presented in this email are solely those of the Author and do not necessarily represent those of the company. Finally, the recipient should check this email and any attachments for the presence of viruses. The Medical Center accepts no liability for any damage caused by any virus transmitted by this email. St. Joseph's Medical Center, 523 N. 3rd street, Brainerd, MN 56401 From twheelock <@t> mclean.harvard.edu Fri Jan 12 12:54:41 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Jan 12 12:34:47 2007 Subject: [Histonet] LUXOL FAST BLUE Message-ID: <45A7D971.6070103@mclean.harvard.edu> Hi Thomas: I stain 5 micron paraffin sections of human brain with Luxol Fast Blue routinely. Forget the Lithium. The best differentiator is a 1% hydroquinone, 5% sodium sulfite aqueous solution. This will pull out the Luxol from everything except the white matter, lipofuscin, and elastic fibers in arteries. Works every time. At least with my thin 5 micron sections, you only need 2 dips in the differentiator. Then immediately into 3 changes of tap water of about 20 dips each, once more in tap water for 1 minute, and then a final holding dish with tap water. Your 7 micron sections should behave the same. If you want to add 1 more dip in the differentiator that should be fine too. As for the pink stain that you have seen, it is probably Eosin. I counter-stain the Luxol with: (1) 10 minutes in Gill 3 Hematoxylin (2) 3 minutes in Eosin Y Alcoholic. So I end with a triple stain, an LHE (Luxol Fast Blue-Hematoxylin-Eosin) The PAS should also work nicely once you differentiate the Luxol. But as I hardly ever do this, I am not sure of the protocol. However Sheehan's "Theory and Practice of Histotechnology" should have a Luxol-PAS recipe. Hopes this helps. I will send the entire protocol and an image to your email. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From PMonfils <@t> Lifespan.org Fri Jan 12 12:52:13 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jan 12 12:52:21 2007 Subject: [Histonet] LUXOL FAST BLUE In-Reply-To: <45A7D971.6070103@mclean.harvard.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BF7@LSRIEXCH1.lsmaster.lifespan.org> I also do the Luxol Fast Blue as described by Tim Wheelock. Just wanted to add that I routinely cut my LFB sections at 10 microns, and the two quick dips in the hydroquinone/sodium sulfite differentiator still works fine. From twheelock <@t> mclean.harvard.edu Fri Jan 12 13:13:55 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Jan 12 12:53:59 2007 Subject: [Histonet] LUXOL DIFFERENTIATOR Message-ID: <45A7DDF3.7070505@mclean.harvard.edu> Hi LuAnn: I put the distilled water on a stirrer. Then slowly add the hydroquinone. Then slowly add the sodium sulfite. Then let it stir for a few minutes until all the reagents are disssolved. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From twheelock <@t> mclean.harvard.edu Fri Jan 12 13:33:10 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Jan 12 13:13:18 2007 Subject: [Histonet] DIFFERENTIATOR RECIPE Message-ID: <45A7E276.6030703@mclean.harvard.edu> Hi LuAnn: I start out with 2 liters of distilled water. Then I add 20 grams of hydroquinone. Then I add 100 grams of soldium sulfite. By the way, make sure that it is sodium sulFITE........not sulFATE Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From JMacDonald <@t> mtsac.edu Fri Jan 12 13:26:45 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jan 12 13:26:14 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <8C90462981F33FA-7CC-2FA@FWM-D35.sysops.aol.com> Message-ID: Most facilities have unique identifier numbers for patients, such as the hospital number. Why not use this in adddition to the surgical number. From mtarango <@t> nvcancer.org Fri Jan 12 13:42:45 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Jan 12 13:43:11 2007 Subject: [Histonet] Re: Block indentifiers Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F98FC@NVCIEXCH02.NVCI.org> We should come up with a coded writing that only histotechs can read. :-D Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 9:23 AM To: Cheryl Kerry; 'Galiotto, Laura'; mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers Consider the following: the blocks are going to be sent out for a consult: how many people unrelated to the lab will be able to read the name in the block? Do those unrelated people have the "right" to read that name? Even those with that "right" could find out about a certain name and make some commentary to another person without the "right" to know. There is a big diference between the "right" to know and the "need to know". A second log number, a date or a bar code are better and anonymous second identifiers. I would not like my name to be written in a cassette. Ren? J. Cheryl Kerry wrote: On confidentiality policies-- These policies are to protect patient information from people who do not have the right to see it. Wouldn't anyone handling blocks have not just the right, but the need to validate the ID of the block to the associated patient report? Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Friday, January 12, 2007 10:51 AM To: Rene J Buesa; mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers Hello Histonetter's I am curious to those that are not using the patient's name as a second identifier. What are you using, since JCAHO is requiring a second identifier? Laura -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, January 12, 2007 8:58 AM To: mhkatie@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Block indentifiers Word of caution about patient's name in cassettes or slides: check you "confidentiality policies" before doing that because the patient's name will be available to any with access to either the cassette or the slide. We never allowed that type of "second" identification. Ren? J. mhkatie@aol.com wrote: Our facility puts the patients last name on the cassettes as the second identifier. Ann Lynde Medicine Hat Diagnostic Laboratory Medicine Hat, Alberta 403-527-3989 Message: 6 Date: Wed, 10 Jan 2007 13:50:02 -0600 From: "Renko, Heather D." Subject: [Histonet] Block identifiers To: Histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777529F@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 I would like some input on handwritten identifiers on blocks/cassettes. Our facility currently puts the surgical number only on our blocks and is considering the idea of a second identifier. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. 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Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From melissa.mazan <@t> tufts.edu Fri Jan 12 14:01:25 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Fri Jan 12 14:01:34 2007 Subject: [Histonet] immunocytochemistry Message-ID: <20070112150125.bxbafrwguo8ogck4@webmail.tufts.edu> Hi all, I haven't done immunofluorescence on cultured cells before, and one of my colleagues would like me to do some - he's grown up the cells on cover slips, then fixed with methanol/acetic acid. They've been stored at room temp for the past week. Prior to staining, do I have to rehydrate them with graded alcohols? or just rinse with PBS or PBS/triton-x? Thanks! Melissa Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu From settembr <@t> umdnj.edu Fri Jan 12 14:14:16 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Jan 12 14:15:07 2007 Subject: [Histonet] fish Message-ID: funny. Dana >>> "Edwards, R.E." 01/12/07 4:30 AM >>> Best served with chips, and if feeling the need for healthy eating, mushy peas. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 11 January 2007 18:17 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish Does anybody know what the regs are for FISH? Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Fri Jan 12 14:31:34 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Jan 12 14:32:05 2007 Subject: [Histonet] fish In-Reply-To: References: Message-ID: How about cheesy peas? >Best served with chips, and if feeling the need for healthy eating, >mushy peas. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >godsgalnow@aol.com >Sent: 11 January 2007 18:17 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] fish > > Does anybody know what the regs are for FISH? > >Roxanne >________________________________________________________________________ >Check out the new AOL. Most comprehensive set of free safety and >security tools, free access to millions of high-quality videos from >across the web, free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From funderwood <@t> mcohio.org Fri Jan 12 14:39:10 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Jan 12 14:39:51 2007 Subject: [Histonet] fish Message-ID: Try to visualize whirled peas. >>> Philip Oshel 1/12/2007 3:31 PM >>> How about cheesy peas? >Best served with chips, and if feeling the need for healthy eating, >mushy peas. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >godsgalnow@aol.com >Sent: 11 January 2007 18:17 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] fish > > Does anybody know what the regs are for FISH? > >Roxanne >________________________________________________________________________ >Check out the new AOL. Most comprehensive set of free safety and >security tools, free access to millions of high-quality videos from >across the web, free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jan 12 14:52:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 12 14:52:32 2007 Subject: [Histonet] immunocytochemistry In-Reply-To: <20070112150125.bxbafrwguo8ogck4@webmail.tufts.edu> Message-ID: <91289.45511.qm@web61212.mail.yahoo.com> Melissa: To start, it would have been better if there would have been stored in a refrigerator (4?C). You do not need to rehydrate them, since methanol just fixed them (like a blood smear), so you can start by placing them in PBS. Ren? J. Melissa Mazan wrote: Hi all, I haven't done immunofluorescence on cultured cells before, and one of my colleagues would like me to do some - he's grown up the cells on cover slips, then fixed with methanol/acetic acid. They've been stored at room temp for the past week. Prior to staining, do I have to rehydrate them with graded alcohols? or just rinse with PBS or PBS/triton-x? Thanks! Melissa Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From pruegg <@t> ihctech.net Fri Jan 12 14:54:28 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jan 12 14:54:44 2007 Subject: [Histonet] frozen tissue Message-ID: <008201c7368b$da597bc0$6501a8c0@Patsy> I am having trouble getting tissue to cut that are prepared as such: "Animals were heparinized through the inferior vena cava under Avertin anesthesia. Hearts were removed, cannulated through the aorta, and reverse perfused with cardioplegia solution (physiological buffer containing high concentrations of KCl and EGTA) for 5 min at 2 ml/min to clear blood and fully relax the heart. Perfusion was then switched to 4% paraformaldehyde in PBS for 5 min. Hearts were removed from the perfusion apparatus, ventricles were dissected free of aorta and connective tissue, and hearts were stored overnight at 4C in cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% ethylene glycol)." I took these tissues and snap froze them in liquid nitrogen in cryomolds filled with OCT as I usually do frozen tissue, as I tried to cut them in the cryostat they seemed raw as if the glycol had effected the freezing, the OCT surrounding the tissue was well frozen but the tissue seemed not so well frozen. Does anyone have experience with freezing tissues that have been place in ethylene glycol cryo protectant, I have not seen this before. Any advice would be appreciated. Some of these tissues are still in 30% sucrose which I transferred them to to try and rinse out the glycol. Thanks, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From jfish <@t> gladstone.ucsf.edu Fri Jan 12 15:14:19 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Jan 12 15:15:04 2007 Subject: [Histonet] frozen tissue In-Reply-To: <008201c7368b$da597bc0$6501a8c0@Patsy> Message-ID: <000001c7368e$9f77c130$8903010a@JFISH> Dear Patsy, Your cryoprotectant solution sounds suspiciously like the solution we use to store vibratomed sections at -20C to actually keep them FROM freezing. It allows us to keep them very cold but without the damage of actually freezing them. The only difference is that we add glycerine to the solution. I suspect the ethylene glycol is the problem, I don't think it will allow the tissue to freeze. Try leaving it out of the mix and just make a 30% sucrose/0.1M phosphate buffered solution. Just my thoughts. Take care, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, January 12, 2007 12:54 PM To: 'histonet' Subject: [Histonet] frozen tissue I am having trouble getting tissue to cut that are prepared as such: "Animals were heparinized through the inferior vena cava under Avertin anesthesia. Hearts were removed, cannulated through the aorta, and reverse perfused with cardioplegia solution (physiological buffer containing high concentrations of KCl and EGTA) for 5 min at 2 ml/min to clear blood and fully relax the heart. Perfusion was then switched to 4% paraformaldehyde in PBS for 5 min. Hearts were removed from the perfusion apparatus, ventricles were dissected free of aorta and connective tissue, and hearts were stored overnight at 4C in cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% ethylene glycol)." I took these tissues and snap froze them in liquid nitrogen in cryomolds filled with OCT as I usually do frozen tissue, as I tried to cut them in the cryostat they seemed raw as if the glycol had effected the freezing, the OCT surrounding the tissue was well frozen but the tissue seemed not so well frozen. Does anyone have experience with freezing tissues that have been place in ethylene glycol cryo protectant, I have not seen this before. Any advice would be appreciated. Some of these tissues are still in 30% sucrose which I transferred them to to try and rinse out the glycol. Thanks, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbanwait <@t> buckinstitute.org Fri Jan 12 15:25:28 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Fri Jan 12 15:25:38 2007 Subject: [Histonet] frozen tissue In-Reply-To: <000001c7368e$9f77c130$8903010a@JFISH> Message-ID: Hi There, Yes we also use this solution to 'cryoprotect' (keep tissue from freezing/forming ice crystals) vibratome free floating sections. When running IHC on these samples , we give the tissue thorough washes with PBS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo Dee Fish Sent: Friday, January 12, 2007 1:14 PM To: 'Patsy Ruegg'; 'histonet' Subject: RE: [Histonet] frozen tissue Dear Patsy, Your cryoprotectant solution sounds suspiciously like the solution we use to store vibratomed sections at -20C to actually keep them FROM freezing. It allows us to keep them very cold but without the damage of actually freezing them. The only difference is that we add glycerine to the solution. I suspect the ethylene glycol is the problem, I don't think it will allow the tissue to freeze. Try leaving it out of the mix and just make a 30% sucrose/0.1M phosphate buffered solution. Just my thoughts. Take care, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, January 12, 2007 12:54 PM To: 'histonet' Subject: [Histonet] frozen tissue I am having trouble getting tissue to cut that are prepared as such: "Animals were heparinized through the inferior vena cava under Avertin anesthesia. Hearts were removed, cannulated through the aorta, and reverse perfused with cardioplegia solution (physiological buffer containing high concentrations of KCl and EGTA) for 5 min at 2 ml/min to clear blood and fully relax the heart. Perfusion was then switched to 4% paraformaldehyde in PBS for 5 min. Hearts were removed from the perfusion apparatus, ventricles were dissected free of aorta and connective tissue, and hearts were stored overnight at 4C in cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% ethylene glycol)." I took these tissues and snap froze them in liquid nitrogen in cryomolds filled with OCT as I usually do frozen tissue, as I tried to cut them in the cryostat they seemed raw as if the glycol had effected the freezing, the OCT surrounding the tissue was well frozen but the tissue seemed not so well frozen. Does anyone have experience with freezing tissues that have been place in ethylene glycol cryo protectant, I have not seen this before. Any advice would be appreciated. Some of these tissues are still in 30% sucrose which I transferred them to to try and rinse out the glycol. Thanks, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Jan 12 15:34:35 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Jan 12 15:37:57 2007 Subject: [Histonet] Re: Block indentifiers References: <5AEC610C1CE02945BD63A395BA763EDE1F98FC@NVCIEXCH02.NVCI.org> Message-ID: <08A0A863637F1349BBFD83A96B27A50A11FFE0@uwhis-xchng3.uwhis.hosp.wisc.edu> On our slides for our 2nd ID we put the patient's birthdate, but with no spaces. I guess it could look like an MR# and throw all those nosy people sifting through charts off track. :) Claire Ingles Mohs Clinic UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tarango, Mark Sent: Fri 1/12/2007 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers We should come up with a coded writing that only histotechs can read. :-D Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 From rjbuesa <@t> yahoo.com Fri Jan 12 15:46:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 12 15:46:07 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A11FFE0@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <434945.40117.qm@web61217.mail.yahoo.com> A very, very clever and unique solution, I would say! Ren? J. Ingles Claire wrote: On our slides for our 2nd ID we put the patient's birthdate, but with no spaces. I guess it could look like an MR# and throw all those nosy people sifting through charts off track. :) Claire Ingles Mohs Clinic UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tarango, Mark Sent: Fri 1/12/2007 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers We should come up with a coded writing that only histotechs can read. :-D Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From ander093 <@t> tc.umn.edu Fri Jan 12 15:51:44 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri Jan 12 15:52:18 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A11FFE0@uwhis-xchng3.uwhis. hosp.wisc.edu> References: <5AEC610C1CE02945BD63A395BA763EDE1F98FC@NVCIEXCH02.NVCI.org> <08A0A863637F1349BBFD83A96B27A50A11FFE0@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <6.2.3.4.0.20070112155013.03a7a488@ander093.email.umn.edu> Is a second ID required? It was my understanding that at this point it is merely a suggestion. Albeit, one that is likely to become a requirement in the future. Has it changed? LuAnn At 03:34 PM 1/12/2007, Ingles Claire wrote: >On our slides for our 2nd ID we put the patient's birthdate, but >with no spaces. I guess it could look like an MR# and throw all >those nosy people sifting through charts off track. :) > >Claire Ingles >Mohs Clinic >UW Hospital and Clinics >Madison WI > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tarango, Mark >Sent: Fri 1/12/2007 1:42 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Re: Block indentifiers > > > >We should come up with a coded writing that only histotechs can read. > >:-D > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Jan 12 16:05:23 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jan 12 16:09:52 2007 Subject: [Histonet] frozen tissue In-Reply-To: <008201c7368b$da597bc0$6501a8c0@Patsy> References: <008201c7368b$da597bc0$6501a8c0@Patsy> Message-ID: <45A80623.6090305@umdnj.edu> Ethylene glycol is the main component of automotive anti-freeze. I would omit it from the cryoprotectant. Geoff Patsy Ruegg wrote: >I am having trouble getting tissue to cut that are prepared as such: > >"Animals were heparinized through the inferior vena cava under Avertin >anesthesia. Hearts were removed, cannulated through the aorta, and reverse >perfused with cardioplegia solution (physiological buffer containing high >concentrations of KCl and EGTA) for 5 min at 2 ml/min to clear blood and >fully relax the heart. Perfusion was then switched to 4% paraformaldehyde >in PBS for 5 min. Hearts were removed from the perfusion apparatus, >ventricles were dissected free of aorta and connective tissue, and hearts >were stored overnight at 4C in cryoprotectant solution (0.1 M phosphate >buffer, 30% sucrose, 30% ethylene glycol)." > >I took these tissues and snap froze them in liquid nitrogen in cryomolds >filled with OCT as I usually do frozen tissue, as I tried to cut them in the >cryostat they seemed raw as if the glycol had effected the freezing, the OCT >surrounding the tissue was well frozen but the tissue seemed not so well >frozen. > >Does anyone have experience with freezing tissues that have been place in >ethylene glycol cryo protectant, I have not seen this before. > >Any advice would be appreciated. Some of these tissues are still in 30% >sucrose which I transferred them to to try and rinse out the glycol. > >Thanks, > >Patsy > > > > > >Patsy Ruegg, HT(ASCP)QIHC > >IHCtech, LLC > >12635 Montview Blvd. Ste.215 > >Aurora, Colorado 80045 > >Phone: 720-859-4060 > >Fax: 720-859-4110 > >pruegg@ihctech.net > >www.ihctech.net > >www.ihcrg.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jwatson <@t> gnf.org Fri Jan 12 16:29:54 2007 From: jwatson <@t> gnf.org (James Watson) Date: Fri Jan 12 16:30:16 2007 Subject: [Histonet] frozen tissue Message-ID: Propylene glycol or Ethylene glycol is used in cryoprotectants for brain when the thick sections are cut on a sliding microtome. It keeps the tissue from freezing completely and the section is lifted off the knife with a paint brush then floated onto a slide. This method can produce extremely nice thick section. If you need the exact protocol I would have to dig it out of my files. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, January 12, 2007 2:05 PM To: Patsy Ruegg Cc: 'histonet' Subject: Re: [Histonet] frozen tissue Ethylene glycol is the main component of automotive anti-freeze. I would omit it from the cryoprotectant. Geoff Patsy Ruegg wrote: >I am having trouble getting tissue to cut that are prepared as such: > >"Animals were heparinized through the inferior vena cava under Avertin >anesthesia. Hearts were removed, cannulated through the aorta, and >reverse perfused with cardioplegia solution (physiological buffer >containing high concentrations of KCl and EGTA) for 5 min at 2 ml/min >to clear blood and fully relax the heart. Perfusion was then switched >to 4% paraformaldehyde in PBS for 5 min. Hearts were removed from the >perfusion apparatus, ventricles were dissected free of aorta and >connective tissue, and hearts were stored overnight at 4C in >cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% >ethylene glycol)." > >I took these tissues and snap froze them in liquid nitrogen in >cryomolds filled with OCT as I usually do frozen tissue, as I tried to >cut them in the cryostat they seemed raw as if the glycol had effected >the freezing, the OCT surrounding the tissue was well frozen but the >tissue seemed not so well frozen. > >Does anyone have experience with freezing tissues that have been place >in ethylene glycol cryo protectant, I have not seen this before. > >Any advice would be appreciated. Some of these tissues are still in >30% sucrose which I transferred them to to try and rinse out the >glycol. > >Thanks, > >Patsy > > > > > >Patsy Ruegg, HT(ASCP)QIHC > >IHCtech, LLC > >12635 Montview Blvd. Ste.215 > >Aurora, Colorado 80045 > >Phone: 720-859-4060 > >Fax: 720-859-4110 > >pruegg@ihctech.net > >www.ihctech.net > >www.ihcrg.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jan 12 16:37:48 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jan 12 16:38:00 2007 Subject: [Histonet] frozen tissue In-Reply-To: <45A80623.6090305@umdnj.edu> Message-ID: <00ab01c7369a$49a91630$6501a8c0@Patsy> I have no way to omit the ethylene glycol as these samples have already been exposed to it, I am trying to find out if I can recover these samples for frozen sectioning. The best I could think of was to rinse them in buffer and reinfiltrate in 30% sucrose hoping to wash out the glycol and rehydrate them so that they will freeze and I can get sections, I will let everyone know how this came out, I am not optimistic about the outcome. Patsy -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Friday, January 12, 2007 3:05 PM To: Patsy Ruegg Cc: 'histonet' Subject: Re: [Histonet] frozen tissue Ethylene glycol is the main component of automotive anti-freeze. I would omit it from the cryoprotectant. Geoff Patsy Ruegg wrote: >I am having trouble getting tissue to cut that are prepared as such: > >"Animals were heparinized through the inferior vena cava under Avertin >anesthesia. Hearts were removed, cannulated through the aorta, and reverse >perfused with cardioplegia solution (physiological buffer containing high >concentrations of KCl and EGTA) for 5 min at 2 ml/min to clear blood and >fully relax the heart. Perfusion was then switched to 4% paraformaldehyde >in PBS for 5 min. Hearts were removed from the perfusion apparatus, >ventricles were dissected free of aorta and connective tissue, and hearts >were stored overnight at 4C in cryoprotectant solution (0.1 M phosphate >buffer, 30% sucrose, 30% ethylene glycol)." > >I took these tissues and snap froze them in liquid nitrogen in cryomolds >filled with OCT as I usually do frozen tissue, as I tried to cut them in the >cryostat they seemed raw as if the glycol had effected the freezing, the OCT >surrounding the tissue was well frozen but the tissue seemed not so well >frozen. > >Does anyone have experience with freezing tissues that have been place in >ethylene glycol cryo protectant, I have not seen this before. > >Any advice would be appreciated. Some of these tissues are still in 30% >sucrose which I transferred them to to try and rinse out the glycol. > >Thanks, > >Patsy > > > > > >Patsy Ruegg, HT(ASCP)QIHC > >IHCtech, LLC > >12635 Montview Blvd. Ste.215 > >Aurora, Colorado 80045 > >Phone: 720-859-4060 > >Fax: 720-859-4110 > >pruegg@ihctech.net > >www.ihctech.net > >www.ihcrg.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From liclarke <@t> u.washington.edu Fri Jan 12 17:44:56 2007 From: liclarke <@t> u.washington.edu (Li Clarke) Date: Fri Jan 12 17:45:04 2007 Subject: [Histonet] New assays From R&D to clinical applications Message-ID: <45A81D78.70308@u.washington.edu> We would appreciate some guidance in bringing a "home-grown" assay into clinical use. 1. What are the guidelines regarding FDA approval? 2. How to determine the appropriate CPT code for billing 3. What are regional prices for these types of tests? Thanks. Li -- *~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~* Li Clarke Administrative Director, Pathology Harborview Medical Center Box 359791 Phone: 206.731.2166 / Fax: 206.731.4821 liclarke@u.washington.edu *~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~* Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail and then destroy all copies of the message and any attachments. Thank you. From nienhuis <@t> ucla.edu Fri Jan 12 18:13:33 2007 From: nienhuis <@t> ucla.edu (nienhuis@ucla.edu) Date: Fri Jan 12 18:13:46 2007 Subject: [Histonet] frozen tissue In-Reply-To: References: Message-ID: <20070112161333.hpqqbqn1log0ok4g@mail.ucla.edu> I would appreciate a copy of the exact protocol. Something similar has been suggested as a way of preserving antigenicity of formalin fixed stored tissue. How thick are these thick sections? Bob Nienhuis UCLA / VA Medical Center Quoting James Watson : > Propylene glycol or Ethylene glycol is used in cryoprotectants for brain > when the thick sections are cut on a sliding microtome. It keeps the > tissue from freezing completely and the section is lifted off the knife > with a paint brush then floated onto a slide. This method can produce > extremely nice thick section. If you need the exact protocol I would > have to dig it out of my files. > > James Watson HT, ASCP > Facilities Manager of Histology > GNF, Genomics Institute of the Novartis Research Foundation > Room C015 > 858-332-4647 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff > McAuliffe > Sent: Friday, January 12, 2007 2:05 PM > To: Patsy Ruegg > Cc: 'histonet' > Subject: Re: [Histonet] frozen tissue > > > Ethylene glycol is the main component of automotive anti-freeze. I would > > omit it from the cryoprotectant. > > Geoff > > Patsy Ruegg wrote: > >> I am having trouble getting tissue to cut that are prepared as such: >> >> "Animals were heparinized through the inferior vena cava under Avertin >> anesthesia. Hearts were removed, cannulated through the aorta, and >> reverse perfused with cardioplegia solution (physiological buffer >> containing high concentrations of KCl and EGTA) for 5 min at 2 ml/min >> to clear blood and fully relax the heart. Perfusion was then switched >> to 4% paraformaldehyde in PBS for 5 min. Hearts were removed from the >> perfusion apparatus, ventricles were dissected free of aorta and >> connective tissue, and hearts were stored overnight at 4C in >> cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% >> ethylene glycol)." >> >> I took these tissues and snap froze them in liquid nitrogen in >> cryomolds filled with OCT as I usually do frozen tissue, as I tried to >> cut them in the cryostat they seemed raw as if the glycol had effected >> the freezing, the OCT surrounding the tissue was well frozen but the >> tissue seemed not so well frozen. >> >> Does anyone have experience with freezing tissues that have been place >> in ethylene glycol cryo protectant, I have not seen this before. >> >> Any advice would be appreciated. Some of these tissues are still in >> 30% sucrose which I transferred them to to try and rinse out the >> glycol. >> >> Thanks, >> >> Patsy >> >> >> >> >> >> Patsy Ruegg, HT(ASCP)QIHC >> >> IHCtech, LLC >> >> 12635 Montview Blvd. Ste.215 >> >> Aurora, Colorado 80045 >> >> Phone: 720-859-4060 >> >> Fax: 720-859-4110 >> >> pruegg@ihctech.net >> >> www.ihctech.net >> >> www.ihcrg.org >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dharclerode <@t> cytoritx.com Fri Jan 12 19:36:27 2007 From: dharclerode <@t> cytoritx.com (Donna Harclerode) Date: Fri Jan 12 19:34:56 2007 Subject: [Histonet] Immunocytochemistry Message-ID: <3DE0F644E093DF4BAE80C254176696A51C7927@mp-mailserver.macropore.com> Message: 9 Date: Fri, 12 Jan 2007 15:01:25 -0500 From: Melissa Mazan Subject: [Histonet] immunocytochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <20070112150125.bxbafrwguo8ogck4@webmail.tufts.edu> Content-Type: text/plain; charset=ISO-8859-1; format="flowed" Hi all, I haven't done immunofluorescence on cultured cells before, and one of my colleagues would like me to do some - he's grown up the cells on cover slips, then fixed with methanol/acetic acid. They've been stored at room temp for the past week. Prior to staining, do I have to rehydrate them with graded alcohols? or just rinse with PBS or PBS/triton-x? Thanks! Melissa Hi Melissa You can stain 24 well plates instead of cover glasses if you want. The coverslips can go directly into PBS- Hopefully they were rinsed after fixing and not air dried with the acetic acid left on. The other problem I have had is fixing cells without rinsing the media with FBS or other kind of serum. That tends to leave a bad haze on my cells. I do not let my cells dry out at anytime. I personally will avoid cover glass staining if possible. I stain the plastic plates or use chamber slides. The same reagents could be used for cover glasses. For the antibodies I stain I have not found methanol to be a good fix, especially the cell membrane markers so I do not use it for anything. In the future I would fix your cells, rinse them and hold them in buffer for up to about a week before staining. Many cells will start to lift off the plate or slide after a week. My preferred antibodies are PharMingen and I also use a lot of Lab Vision. Here is the "cheat sheet" I give everyone here- it covers all the IF we do. We stain many plates (1-14 at a time with 1-6 different antibodies per plate) with excellent results. I do cover them when in secondary and store them in the fridge, usually in the cardboard Revco boxes that fit 2 or 3 plates. The formatting got lost so if you want an easier copy to read, let me know and I will attach one. Good luck! Donna Donna Harclerode, HT, (ASCP), HTL, QIHC Scientist / Immunohistochemistry Cytori Therapeutics 3020 Callan Rd. San Diego, CA 92121 858-458-0900 ext 5416 fax 858 200-0945 dharclerode@cytoritx.com Basic Fluorescent Immunocytochemistry for Plates or Chamber Slides DAB on final page Remove media from live cells 1. Rinse live cells in media containing no serum or in PBS 1 times 1-5 minutes. 2. Fix cells in 4% PFA made from concentrate (32% Electron Microscopy Science) using PBS as a diluent for 15 minutes and remove fixative 3. Rinse in PBS 3 time 5 minutes each 4. Make up correct dilution for primary antibody in Dako Antibody diluent (I do not use serum usually in any antibody solution but you can add 5% Normal Donkey serum to the diluent used for the primary antibody- all my secondaries are made in donkey) Just prior to adding antibody gently blot the plates on paper towel to limit dilution of antibodies. and add 100 ul to each chamber on a 24 well plate to be stained. Rotate the plate to be sure the entire well is covered with reagent. FOR OVERNIGHT CHAMBER SLIDES- USE A HUMID CHAMBER AND 100ul per well on a 4 well chamber slide or a 24 well plate or 200ul on a 2 chamber slide TC plates also benefit from wet paper towels being placed on top of each plate 5. Place in 4oC Fridge overnight. Alternately you can use a rocker at room temperature for 2 hours. 6. Remove the primary antibody and rinse 3 times in PBS each for 5 minutes (10 minutes for each rinse may be preferred) 7. Incubate with secondary fluorophore made against the species that the primary antibody is made in and incubate on rocker for 30 minutes to 1 hour. All secondaries are from Jackson ImmuoResearch diluted at 1:100 with Dako Antibody diluent (NO serum) (up to 1:200 dilution of secondary can be used). If desired add 1ul of DAPI solution (frozen aliquots) for each ml of secondary for nuclear detection Unless otherwise requested for specific experiments use whole IgG secondaries. Fab' fragment secondaries have sometimes shown not to be as bright as whole IgG. If there is possible Fc receptor interaction a small comparison study could be used to see if the Fab' secondary decrease background. Whole molecule secondaries are much less expensive than Fab' fragment 8. Rinse plates 3 times with PBS each for 5 minutes 9. Plates can be viewed without coverslipping if desired or a mounting media made for fluorescent dyes may be used. 10. For chamber slides remove the collar rinse again in PBS and coverslip with Aqua Mount or other mountant for fluorescent preparations 11. Slides may be sealed with nail polish, but this will prevent removal of the coverslip and remounting if there are problems with the slide. I highly recommend not ringing the aqueous sections with nail polish for this reason. If they are coverslipped correctly and kept flat in the folders, the covers slips stay where they belong Notes: Do not allow plates to dry out at any time. Adding different antibodies to 24 well plates requires planning and concentration. A rocking plate is used for all rinses and room temperature incubations. An orbital rocker should not be used, as the center of each chamber is likely to have less contact with the antibody >From the secondary antibody (fluorophore) on, keep the slides or plates from direct light exposure. It is not necessary to seal them in foil, but blocking the overhead light with a box or towel is good. Antibody concentrations used for fluorescent tissue sections IHC staining will be close if not exactly the correct concentration for chamber slides Dako diluent contains both a non serum block for background and a detergent for permeablization of the cells. The non serum block will prevent artifact staining of Fc receptors and secondary cross reactivity with the primary. Add antibody and PBS gently on the side of the well so the cells will remain adherent to plate or slide. To remove reagents use an aspirator or just gently turn the plates onto clean papers towels and blot gently. Results tend to be crisper when primary antibody is incubated overnight at 4oC than 2 hours at room temp Do not use any permanent mounting media containing alcohol or other solvent on fluorescent preparations. Chamber slides can have the collars removed before fixation if the antibodies require acetone or any other solvent based fixative. They must then be stained in humid chamber flat and cannot be used with the Sequenza system used for standard slides because of the dividers on the slides. The unfixed chamber slides are very fragile, they must be treated very gently or cells will be knocked off the slide. Any or all PBS rinses can be increased to10 minutes or more if convenient or if any type of matrix is used with the cells. Slides or plates may be stored at 4oC in the fridge for up to 2 months. Some signals may last longer, but these will be on a case by case basis Alternate fixatives to 4% PFA can be used. Acetone -Ethanol 50% of each can be used in plates. We did not find a significant improvement to date with any abs we tested. Methanol is also recommended by some papers. I have found serious problems with methanol fixation and would try it only as a last resort. Ideally plates will be stained immediately after fixation. Plates can be fixed and then stored in PBS after 3 washers at 4 oC for up to one week. Prepared antibodies are stable if made in Dako diluent and stored at 4oC for at least 1 month. Secondary preparations are stable for at least 2 weeks if kept at 4oC and light protected. Step 1-6 Rinse cells, fix plates, wash with PBS 3 times 5 minutes 7. Add 2-3 drops per plate of HRP block (Dako Endogenous Peroxidase block) if there is any chance that there will be RBCs or other HRP cells (granulocytes) for 10 minutes 8. Rinse 3x with PBS (if you have cells with endogenous biotin, you will also need to block for that) 9 Add 100ul properly diluted primary ab to a 24 well plate and incubate on rocker for 1-2 hours 7. Rinse Plate 3 times each for 5 minutes with PBS 8. Add 100ul of secondary antibody to each well (unless using a biotinolated primary) made in Dako diluent at 1:200 biotin anti mouse or rabbit or whatever and incubate for 30 minutes on the rocker 9 Rinse the plates 3 times for 5 minutes each 10. Add 2-3 drops LSAB (labeled strep avidin biotin) to each well and incubate 30 minutes on a rocker 11. Rinse 3 times with PBS each for 5 minutes 12 Always wear gloves and perform the DAB step on paper that will be disposed of after use. It will dye your clothes and is a mild carcinogen. Make up DAB solution 1 ml diluent 1 drop reagent. 4-5 ml is a good amount for a 24 well plate 13 Add 3-5 drops of DAB per well to stain the reactive cells. If all wells are not done at the same time, keep buffer in the well and do not allow to dry out. Stain from 30 seconds to 5 minutes (usual is 1-2 minutes. Rinse plates well in DI water Counterstain with Gill 1 hematoxylin if desired 5-10 seconds Rinse with DI water Blue with Bluing reagent (or sodium bicarbonate of other mild base) Rinse well with DI water and allow drying. DI water can be added to plates if desired for viewing or visualized dry. Do not store wet plates long term as they will eventually grow mold. From akemiat3377 <@t> yahoo.com Fri Jan 12 19:07:48 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jan 12 20:10:04 2007 Subject: [Histonet] Joint guideline on testing for HER2 status in invasivebreast cancer In-Reply-To: References: Message-ID: Leave it to Vinnie to pull the rabbit out of the hat! Akemi ? Specializing in Histology, SS, IHC, & Microarray Akemi Allison-Tacha BS, HT (ASCP) HTL President Madison, WI Cell: (925) 788-0900 E-Mail: akemiat3377@yahoo.com On Jan 12, 2007, at 11:42 AM, Vinnie Della Speranza wrote: > Douglas, > I have not read through the guideline which is quite a lengthy > document but I'll comment on your questions with my best guess. > others who've thoroughly studied the guideline are free to disagree. > > my understanding and what I believe is the basis for the creation > of this guideline is that previous lack of correlation of Her 2 > results when comparing needle core results with lumpectomy. Our > needle cores arrive in formalin and given their relatively small > size, concern about adequate fixation of needle cores is much less > likely to arise than the lumpectomy samples, which arrive unfixed > and may sit before being grossed in and placed into formalin. This > variation is largely the root cause of a failure of results to > correlate between needle cores and the lumpectomy specimen, in my > opinion. > > In addition, it might be difficult for most labs to determine with > any degree of accuracy exactly how much fixation time a needle core > received since the lab can't control when the sample is introduced > into formalin. not so with lumpectomy samples. > > I believe the standard intends that lumpectomy specimens arrive at > Pathology fresh and that these are to be cut down and placed in > fixative with minimum delay. in addition, minimum and maximum > fixation times are proposed (no less than six hours in formalin, no > more than 48). if you typically start your processor on Friday > evening with a delay start with tissues sitting in formalin, this > upper fixation limit may be an issue for you. > > in regard to your question " Does this mean that the sample that is > not placed in cassettes is not to be fixed?" the answer is NO. they > are just insisting that specimens be cut down to reasonable > thicknesses before immersion in formalin. > > Vinnie > > > > > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, SC 29425 > Ph: 843-792-6353 > fax: 843-792-8974 > >>>> "Douglas D Deltour" 01/12/07 11:10AM >>> > Has anyone had a chance to decipher this new guideline for HER2 > testing? I > think that overall it is a good idea but I have an issue with > appendix E. It > says that "Fixation times for needle biopsies have not been > addressed." Why > put out a guideline when the initial HER2 testing is done on the > needle > biopsy? > > > > It also says "Breast specimens, after appropriate gross inspection and > designation of margins, should be promptly sliced at 5- to 10-mm > intervals > and fixed in formalin (unsliced samples should not be fixed). The > interval > between tissue acquisition and fixation of > > breast specimens should be as short as possible." Am I off on this > one by > thinking that "unsliced samples should not be fixed" means that the > breast > should be delivered fresh? Does this mean that the sample that is > not placed > in cassettes is not to be fixed? What do you do with the unsliced/ > unfixed > sample then? I need some coffee! Someone straighten me out. > > > > > > http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf > > > > Douglas D. Deltour HT(ASCP) > > Histology Supervisor > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > (803)252-1913 > > Fax (803)254-3262 > > > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or > entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If > the reader > of this message is not the intended recipient, you are hereby > notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Jan 12 20:50:04 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jan 12 20:50:22 2007 Subject: [Histonet] Re: Block identifiers Message-ID: Could somebody provide chapter and verse for this new JCAHO reg about having two identifiers on every paraffin block? It's important for us to see the exact wording, and anyway JCAHO has become such a bogeyman that it frequently spawns urban legends. Mark Tarango suggests: >>We should come up with a coded writing that only histotechs can read.<< May I suggest Shaw Alphabet - those of us on the listserv think there are about 150 people who can read it - see my page http://members.aol.com/RSRICHMOND/shavian.html Bob Richmond Samurai Pathologist Knoxville TN From lscott <@t> sfcn.org Fri Jan 12 21:15:33 2007 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Fri Jan 12 21:15:56 2007 Subject: [Histonet] Sakura Tape Coverslipper Message-ID: <000c01c736c1$16693490$0200a8c0@scott> Has anyone used the sakura tape coverslipper that can answer a question for me? Scott Hendricksen HT (ASCP) From lscott <@t> sfcn.org Fri Jan 12 21:31:02 2007 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Fri Jan 12 21:31:30 2007 Subject: [Histonet] Sakura Tape coverslipper Help Message-ID: <003701c736c3$3ff3bf90$0200a8c0@scott> I am having a problem with the tape leaving brownish dry spots on harder tissues and even some smaller skin tags. We are using plenty of xylene on each slide. Has anyone seen this Problem using the tape? I am wondering if our xylene is the problem. The xylene we are using is good quality, but it is called XYLENES with an "S" on the end. The coverslipper is brand new and functions great. I just can't figure out the dry spots in the tissue. Plase Help ! Scott Hendricksen HT(ASCP) From rjbuesa <@t> yahoo.com Sat Jan 13 07:35:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 13 07:35:49 2007 Subject: [Histonet] Sakura Tape coverslipper Help In-Reply-To: <003701c736c3$3ff3bf90$0200a8c0@scott> Message-ID: <243033.22635.qm@web61219.mail.yahoo.com> All "xylene" bottles are labelled "xylenes" because they are always a mixture of the forms "ortho", meta" and "para" xylenes (depending on the bonding site), so never mind the "s". I could only think in the possibility of dust particles (provided that you are using the original tape from Sakura). My film coverslippers were always inside a fume hood, and I never experiences those spots you are referring to. Ren? J. Scott Hendricksen wrote: I am having a problem with the tape leaving brownish dry spots on harder tissues and even some smaller skin tags. We are using plenty of xylene on each slide. Has anyone seen this Problem using the tape? I am wondering if our xylene is the problem. The xylene we are using is good quality, but it is called XYLENES with an "S" on the end. The coverslipper is brand new and functions great. I just can't figure out the dry spots in the tissue. Plase Help ! Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From bayoubelle311 <@t> gmail.com Sat Jan 13 11:02:33 2007 From: bayoubelle311 <@t> gmail.com (Missy) Date: Sat Jan 13 11:02:42 2007 Subject: [Histonet] job reqs Message-ID: <398f02c20701130902q61277904o5ea1170ecc58ec12@mail.gmail.com> Question... in the state of Florida is it necessary to hold a license before you can qualify for a histo position? I currently have a bachelor degree plus some graduate work and some non-clinical histopath experience From rjbuesa <@t> yahoo.com Sat Jan 13 12:05:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 13 12:05:26 2007 Subject: [Histonet] job reqs In-Reply-To: <398f02c20701130902q61277904o5ea1170ecc58ec12@mail.gmail.com> Message-ID: <20070113180519.86439.qmail@web61216.mail.yahoo.com> Hiring in Florida requires a license already issued OR if you have applied for it (if you qualify) while waiting to take the examination (provided that you pass). Permanent hiring is conditioned to passing the examination. The bachelor degree by itself does not qualify to work as a histotech. Ren? J. Missy wrote: Question... in the state of Florida is it necessary to hold a license before you can qualify for a histo position? I currently have a bachelor degree plus some graduate work and some non-clinical histopath experience _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From suetrant <@t> telus.net Sat Jan 13 12:41:02 2007 From: suetrant <@t> telus.net (Sue Trant) Date: Sat Jan 13 12:35:16 2007 Subject: [Histonet] sakura coverslipper Message-ID: <000f01c73742$602e6150$0200a8c0@ROGER> Does everyone routinely have there coverslipper in a fume hood? Our coverslippers seems to give off a fair amount of xylene fumes. We do have a ventilation system, but I am wondering how much damage we are doing to ourselves. Sue Trant Histotechnologist -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.16.10/624 - Release Date: 2007-01-12 From mbmphoto <@t> gmail.com Sat Jan 13 12:57:57 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sat Jan 13 12:56:49 2007 Subject: [Histonet] frozen tissue In-Reply-To: References: Message-ID: <93EB7932-1F47-4A1E-A7EF-FCB482F1D79E@gmail.com> Hello, In our lab, we lots (LOTS) of large (3X2.5 inche) primate brain blocks & rat whole brains Here's what we do to get beautiful 40um cryosections. The primate brain blocks are: 1. fixed. 2. washed X2 in working PBS to remove residual fixative and then 3. placed in cryoprotectant solution of PBS & 30 % sucrose until the tissue sinks to the bottom of container @ 4C. This takes several days. 4. tissue blocks are blotted using kimwipes to remove excess sucrose (before) freezing using the liquid nitrogen & metal block method. We use this method of freezing primate blocks because we absolutely required that our tissue have a flat cutting surface. 5. after freezing the blocks are wrapped twice w/foil & placed in freezer zip-loc bag w/silca gel and packed into cryo boxes and stored at -80C freezer - to be cut later on the cryostat. Now for mice or rat brains: 1. after cryoprotectant solution, again whole brains are blotted using kimwipes to remove excess sucrose and then frozen on a cryo chuck using cryo-gel (we don't use OCT - too running). Beside cryo-gel holds much better and keeps tissue colder. 2. during sectioning for both primate & rat brain, we use anti-freeze solution in culture plate well to place serial sections. The anti-freeze solution has ethylene glycol/ glycerin (99%)/PBS. 3. we use a sable brush to wet the knife edge w/anti-freeze solution. As a section is cut, it slides on top of the knife w/anti-freeze solution. Just simply use the brush to lift the section off the knife & place into a well with more anti-freeze solution. **after learning this technique - it's fast, easy to section tissue and not as messy as you might think. 4. the plate wells full of cut sections in anti-freeze solution are sealed with cryo-tape and stored at 4C temp in plastic boxes. 5. we do IHC/IFA on these sections including H&E and Nissl staining - even on those sections stored for several months. **Now, if you need to store the sections for long term in anti-freeze solution at 4C - add a few grains of thymol into the solution as a preservative. I hope this helps. Maria Bartola Mejia Department of Neurosurgery UCSF San Francisco, CA On Jan 12, 2007, at 2:29 PM, James Watson wrote: > Propylene glycol or Ethylene glycol is used in cryoprotectants for > brain > when the thick sections are cut on a sliding microtome. It keeps the > tissue from freezing completely and the section is lifted off the > knife > with a paint brush then floated onto a slide. This method can produce > extremely nice thick section. If you need the exact protocol I would > have to dig it out of my files. > > James Watson HT, ASCP > Facilities Manager of Histology > GNF, Genomics Institute of the Novartis Research Foundation > Room C015 > 858-332-4647 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff > McAuliffe > Sent: Friday, January 12, 2007 2:05 PM > To: Patsy Ruegg > Cc: 'histonet' > Subject: Re: [Histonet] frozen tissue > > > Ethylene glycol is the main component of automotive anti-freeze. I > would > > omit it from the cryoprotectant. > > Geoff > > Patsy Ruegg wrote: > >> I am having trouble getting tissue to cut that are prepared as such: >> >> "Animals were heparinized through the inferior vena cava under >> Avertin >> anesthesia. Hearts were removed, cannulated through the aorta, and >> reverse perfused with cardioplegia solution (physiological buffer >> containing high concentrations of KCl and EGTA) for 5 min at 2 ml/min >> to clear blood and fully relax the heart. Perfusion was then >> switched >> to 4% paraformaldehyde in PBS for 5 min. Hearts were removed from >> the >> perfusion apparatus, ventricles were dissected free of aorta and >> connective tissue, and hearts were stored overnight at 4C in >> cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% >> ethylene glycol)." >> >> I took these tissues and snap froze them in liquid nitrogen in >> cryomolds filled with OCT as I usually do frozen tissue, as I >> tried to >> cut them in the cryostat they seemed raw as if the glycol had >> effected >> the freezing, the OCT surrounding the tissue was well frozen but the >> tissue seemed not so well frozen. >> >> Does anyone have experience with freezing tissues that have been >> place >> in ethylene glycol cryo protectant, I have not seen this before. >> >> Any advice would be appreciated. Some of these tissues are still in >> 30% sucrose which I transferred them to to try and rinse out the >> glycol. >> >> Thanks, >> >> Patsy >> >> >> >> >> >> Patsy Ruegg, HT(ASCP)QIHC >> >> IHCtech, LLC >> >> 12635 Montview Blvd. Ste.215 >> >> Aurora, Colorado 80045 >> >> Phone: 720-859-4060 >> >> Fax: 720-859-4110 >> >> pruegg@ihctech.net >> >> www.ihctech.net >> >> www.ihcrg.org >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From themagoos <@t> rushmore.com Sat Jan 13 15:22:37 2007 From: themagoos <@t> rushmore.com (Jason and Heather) Date: Sat Jan 13 15:23:28 2007 Subject: [Histonet] Sakura Tape coverslipper Help References: <003701c736c3$3ff3bf90$0200a8c0@scott> Message-ID: <002201c73758$f4242e10$102c9e43@magoo> We have had this problem many times. It seems that the culprit is H2O in the Xylene in the staining row or the Xylene that is used before coverslipping. Our solution is more frequent xylene changes. Hope this helps you. Jason McGough, HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th St. Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com ----- Original Message ----- From: "Scott Hendricksen" To: Sent: Friday, January 12, 2007 8:31 PM Subject: [Histonet] Sakura Tape coverslipper Help I am having a problem with the tape leaving brownish dry spots on harder tissues and even some smaller skin tags. We are using plenty of xylene on each slide. Has anyone seen this Problem using the tape? I am wondering if our xylene is the problem. The xylene we are using is good quality, but it is called XYLENES with an "S" on the end. The coverslipper is brand new and functions great. I just can't figure out the dry spots in the tissue. Plase Help ! Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ohenry <@t> dfw.net Sat Jan 13 16:08:35 2007 From: ohenry <@t> dfw.net (Susan Owens) Date: Sat Jan 13 16:11:52 2007 Subject: [Histonet] RE: Sakura Tape coverslipper Help Message-ID: <001401c7375f$c5e4de80$fdf763d8@your4f1261a8e5> >Subject: [Histonet] Sakura Tape coverslipper Help >I am having a problem with the tape leaving brownish dry spots on harder >tissues and even some smaller skin tags. We are using plenty of xylene on >each slide. >Has anyone seen this Problem using the tape? I am wondering >if our xylene is the problem. The xylene we are using is good quality, but >it is called XYLENES >with an "S" on the end. The coverslipper is brand >new and functions great. I just can't figure out the dry spots in the >tissue. Plase Help ! >Scott Hendricksen HT(ASCP) Yes Scott, we've had the same problem for years. BUT ONLY on 'knee' specimens. We tried everything we could think of. Nothing seemed to solve the problem. And it ONLY effects our 'knee' sections. For us, no other tissue from any part of the body is effected. Yes it is a drying, you can actually see it spread across the section if you catch it early.... Since it only effects our 'knee' sections, we bunch the 'knee' slides, stain them together, and hand coverslip them. No problem with the hand coverslipping.... New xylene, distilled xylene, length of time in xylene, thickness of section, nothing we looked into corrected the problem...Now I will say that I seem to remember that ultra thin sections took longer for the effect to happen, but in the end, even the thinnest knee sections showed the drying effect to some degree. Sorry I don't have better news, maybe someone else in HistoLand knows the answer. Susan From barry_m <@t> ozemail.com.au Sat Jan 13 19:48:37 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Sat Jan 13 19:48:50 2007 Subject: [Histonet] cd8 In-Reply-To: <9e5d1b400701101157v14a78adg901d176946e73707@mail.gmail.com> References: <9e5d1b400701101157v14a78adg901d176946e73707@mail.gmail.com> Message-ID: <000601c7377e$1c62ea40$0201010a@WORKSTATION1> We use the CD8 antibody clone 4B11 from Novocastra. Pre treatment using a High pH heat retrieval solution, and at a dilution of 1:40 On the BONDMAX Immunostainer 15 minutes in Primary and using the VisionBiosystem Define Detection System. Regards Barry Madigan QHPS-Central Royal Brisbane Hospital Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heinrich Lob Sent: Thursday, 11 January 2007 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cd8 Hi, does anybody know where I could find CD8 anti-mouse for IHC-paraffin? Heinrich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barry_m <@t> ozemail.com.au Sat Jan 13 19:48:37 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Sat Jan 13 19:48:54 2007 Subject: [Histonet] HSV ! and II In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2EE4@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2EE4@sjhaexc02.sjha.org> Message-ID: <000701c7377e$1ced28e0$0201010a@WORKSTATION1> Sorry it has taken me so long to respond to this Post but time just seems to fly. We have been using a cocktail of HSV1 and HV2 for 8 years or more now here at the Royal Brisbane Hospital using both DAKO antibodies with excellent results. The current method is as follow: A cocktail made of the following DAKO antibodies Polyclonal HSV 1 1:400 and DAKO Polyclonal HSV 2 1:800 No pre treatment. Used on the BONDMAX Immunostainer. [15 minutes in Primary (no heat) with the VisionBiosystem Define Polymer detection system] Regards Barry Madigan Immunohistochemistry QHPS- Central Royal Brisbane Hospital Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Saturday, 6 January 2007 7:42 AM To: Histonet Subject: [Histonet] HSV We have used combined HSV I & II from Biogenex. It has been on backorder and now may be discontinued. We found an RUO from Biocare - which we want to avoid. We can get I and II separately from DAKO and mix them. What are you all doing? Is there a combined non ASR or RUO somewhere? Have a good weekend and thanks for your help, Joyce Joyce Weems Saint Josephs Hospital of Atlanta From sclark59 <@t> hotmail.com Sun Jan 14 13:37:04 2007 From: sclark59 <@t> hotmail.com (Stephen Clark) Date: Sun Jan 14 13:35:36 2007 Subject: [Histonet] DAB brdU protocol Message-ID: Does anyone have a protocol for DAB staining, specifically for mouse OE sections at about 10 or 18um using brdU? I had been using fluorescent staining procedures, but i wanted to try something non-flourescent to elimate any problems with autoflourescence. I have tried it a few times already with a rough protocol supplied by a colleague with little success. From rjbuesa <@t> yahoo.com Sun Jan 14 13:52:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 14 13:52:40 2007 Subject: [Histonet] DAB brdU protocol In-Reply-To: Message-ID: <85194.94093.qm@web61216.mail.yahoo.com> Stephen: Although I do not know what "OE" stands for, I assume that with "DAB" you are referring to "Di-Amino-Benzidine". If this is the case, DAB is the chromogen that you use AFTER completing the whole IHC (ImmunoHistoChemistry) procedure, meaning that you will have to use first a primary antibody (to the specific antigen you want to detect), followed by the detection system you want to use. This detection step is not necessary for fluorescence protocols. If this is the case you need the protocol for the whole procedure, not just for the final step. If what you have been using are fluorescent markers (FITCI conjugated) I really do not know how you can make the DAB react with those FITCI conjugated antibodies. I don't know if this helps you. Ren? J. Stephen Clark wrote: Does anyone have a protocol for DAB staining, specifically for mouse OE sections at about 10 or 18um using brdU? I had been using fluorescent staining procedures, but i wanted to try something non-flourescent to elimate any problems with autoflourescence. I have tried it a few times already with a rough protocol supplied by a colleague with little success. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From sharon.osborn <@t> comcast.net Sun Jan 14 17:04:24 2007 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Sun Jan 14 17:04:34 2007 Subject: [Histonet] setting up lab Message-ID: <011420072304.1404.45AAB6F8000682210000057C2207021553029D010D9C01D202019D0E089C@comcast.net> I wholeheartedly agree with Rene about the Sakura tissue processor and the Leica cryostat. Both are the workhorse standards and reliable ones for the industry. Sharon Osborn Fremont, CA Date: Thu, 11 Jan 2007 13:55:42 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] thermo vs microm To: Derek Papalegis , histonet@lists.utsouthwestern.edu Message-ID: <801835.60510.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Had I been in your position I would buy a Sakura tissue processor and a Leica cryostat. Reni J. Derek Papalegis wrote: Hi All, So I'm in the process of setting up my new lab and am meeting with all kinds of sales reps. Today was the Thermo guy. We liked his products and of course, according to him they are all 100 times better than the competition. We are very impressed with Richard Allens microm line, especially for cryostats and microtomes. Does anybody have any feedback as to which would they prefer? I know Thermo bought Richard Allen recently so it might not matter in the future but any feedback on whether it is well worth the money to spend the extra for microm products would be helpful. thanks, Derek From sharon.osborn <@t> comcast.net Sun Jan 14 17:15:44 2007 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Sun Jan 14 17:15:53 2007 Subject: [Histonet] TTES Train To End Stroke Message-ID: <011420072315.12932.45AAB9A00005745D000032842207021553029D010D9C01D202019D0E089C@comcast.net> HistoNetters, Several weeks ago, I posted that I was training to do a half marathon for The American Stroke Association. I DID IT! Sunday, Jan 7th, in the Orange County Marathon and Half Marathon, I walked the 13.1 miles in 3 hours 45 minutes! That is 17.17 min/mile average. It was an awesome feeling to complete it! A lot of hard work and training which I am so glad I perservered through. All in all we raised about $18,000 for the American Stroke Association and are still taking donations at http://www.bayarea.kintera.org/oc2006/sosborn I encourage any of you who are thinking about participating in a physical activity to raise money for a great cause to do it. You will definitely benefit physically, have fun and raise money for your cause. For me, this was a great start to 2007. May your start be as meaningful for you! Sharon Osborn Fremont, CA From cytologer <@t> msn.com Sun Jan 14 23:55:09 2007 From: cytologer <@t> msn.com (Choi Ul Soo) Date: Sun Jan 14 23:55:20 2007 Subject: [Histonet] TEM for paraffin embedded tissue section?? Message-ID: Hello Dear Histonetters! would anyone there tell me the story about using paraffin embedded tissue section for TEM analysis? I have seen several reports reading that they used paraffin embedded tissues for transmisison electron microscopy, but our technician is not familiar to this method. Would the results be consistent regardless of the objects of interest, fixation or tissues? I'd like to get findings indicative of neuroendocrine origins from a canine lung mass fixed in formalin, and paraffin embedded tisse section. Please share your experiences and give me advice. Any inputs would be very much appreciated. Ul Soo Choi DVM, PhD VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742 Tel. 82-02-880-8688 Mobile. 82-016-9228-8634 Fax. 82-02-880-8662 _________________________________________________________________ ?? ?? ?? ??? ?????? MSN ???? ?????. http://fortune.msn.co.kr/ From ree3 <@t> leicester.ac.uk Mon Jan 15 03:50:25 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Jan 15 05:59:51 2007 Subject: [Histonet] Re: Block indentifiers In-Reply-To: <434945.40117.qm@web61217.mail.yahoo.com> Message-ID: I have successfully employed such a strategy for many years as nobody can read my hand writing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 12 January 2007 21:46 To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers A very, very clever and unique solution, I would say! Ren? J. Ingles Claire wrote: On our slides for our 2nd ID we put the patient's birthdate, but with no spaces. I guess it could look like an MR# and throw all those nosy people sifting through charts off track. :) Claire Ingles Mohs Clinic UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tarango, Mark Sent: Fri 1/12/2007 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Block indentifiers We should come up with a coded writing that only histotechs can read. :-D Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hli <@t> ion.ac.cn Mon Jan 15 04:27:40 2007 From: hli <@t> ion.ac.cn (Hao Li) Date: Mon Jan 15 06:26:02 2007 Subject: [Histonet] Cryostats for insect neuroanatomy? Message-ID: <002001c7388f$c9513650$e9a80a0a@imohas> Hello, I want to do silver staining in Drosophila and I have looked at a number of published protocols. One thing I have noticed is that practically all of them use paraffin or plastics sectioning. The problem is that we have only a cryostat in the lab. So is there anything wrong with cryostats? For example, I have been worrying that perhaps the 30% sucrose treatment will shrink and distort the tissue? Thanks for your help! Hao Li Institute of Neuroscience, CAS From sonya.martin <@t> soton.ac.uk Mon Jan 15 07:43:07 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Jan 15 07:44:31 2007 Subject: [Histonet] Tumour endothelium - Help! Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34D7@ISS-CL-EX-V1.soton.ac.uk> Hi all, I'm working on some human tumours which have been grown in mice and I want to stain the endothelium. Someone gave me an aliquot of tomato lectin which they use for brain tissue. I tried it on two types of tumour without much success (both were FFPE); human melanoma in mouse lung - stained the alveoli and inside layer of the bronchioles, little staining of the melanoma human B cell lympoma - staining throughout the tumour but no staining of what looks like blood vessels Does anyone have any experience using tomato lectin? Does this sound like background staining of does the tomato lectin bind to other cell types? What does anyone else use for endothelium staining of tumours? Thanks Sonya From contact <@t> excaliburpathology.com Mon Jan 15 08:41:10 2007 From: contact <@t> excaliburpathology.com (P Pierce) Date: Mon Jan 15 08:41:20 2007 Subject: [Histonet] TEM for paraffin embedded tissue section?? Message-ID: <625372.19286.qm@web50112.mail.yahoo.com> Paraffin sections will not survive the electron beam. You will need to melt out the paraffin and dissolve out the remaining paraffin in the tissue with xylene. After removing the paraffin, the tissue will need to be taken back through alcohols and rehydrated. Then it will need to be post-fixed in osmium and processed through to a plastic embedding media such as epon or araldite. The micrograph quality will not be as good as the tissue initially being fixed in gluteraldehyde, but it can be done. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From rjbuesa <@t> yahoo.com Mon Jan 15 09:30:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 15 09:30:38 2007 Subject: [Histonet] TEM for paraffin embedded tissue section?? In-Reply-To: Message-ID: <154576.40622.qm@web61211.mail.yahoo.com> Dear Choi: In extreme cases (then there was no more material in the block) we have done that. Place the the embedding cone (with the tip removed) with the not cured plastic over the area of the section you want to process. Holding both secure together, cure the plastic as usual. When curing is completed remove the cone that will now include the area of the section stuck to the cured resine. With extreme care orient the cone and section it with the ultramicrotome. Stain as usual with your opaque stain. Fixation will not be ideal, but some useful images can be obtained. Hope this will help you. Ren? J. Choi Ul Soo wrote: Hello Dear Histonetters! would anyone there tell me the story about using paraffin embedded tissue section for TEM analysis? I have seen several reports reading that they used paraffin embedded tissues for transmisison electron microscopy, but our technician is not familiar to this method. Would the results be consistent regardless of the objects of interest, fixation or tissues? I'd like to get findings indicative of neuroendocrine origins from a canine lung mass fixed in formalin, and paraffin embedded tisse section. Please share your experiences and give me advice. Any inputs would be very much appreciated. Ul Soo Choi DVM, PhD VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742 Tel. 82-02-880-8688 Mobile. 82-016-9228-8634 Fax. 82-02-880-8662 _________________________________________________________________ ???? ???? ???? ?????? ??????????? MSN ???????­ ??????????. http://fortune.msn.co.kr/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From rjbuesa <@t> yahoo.com Mon Jan 15 09:33:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 15 09:33:17 2007 Subject: [Histonet] Tumour endothelium - Help! In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E34D7@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <959454.23291.qm@web61220.mail.yahoo.com> My experience is with Ulex europaeus lectin. Would you consider using it instead of the "tomato" lectin? If so I can send you my protocol. Ren? J. "Martin S." wrote: Hi all, I'm working on some human tumours which have been grown in mice and I want to stain the endothelium. Someone gave me an aliquot of tomato lectin which they use for brain tissue. I tried it on two types of tumour without much success (both were FFPE); human melanoma in mouse lung - stained the alveoli and inside layer of the bronchioles, little staining of the melanoma human B cell lympoma - staining throughout the tumour but no staining of what looks like blood vessels Does anyone have any experience using tomato lectin? Does this sound like background staining of does the tomato lectin bind to other cell types? What does anyone else use for endothelium staining of tumours? Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jan 15 10:00:14 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jan 15 10:00:24 2007 Subject: [Histonet] guidelines Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640916@hpes1.HealthPartners.int> With the new Her2 guidelines from CAP, how is everyone planning on handling not using alcoholic fixation of fatty breast tissue? We have been using Davidson's on mastectomy and lumpectomy specimens (not needle bx.s) and I have a dedicated processor with the first two stations of an alcoholic formalin fixative. My pathologists want these breast specimens to not be placed in these type of fixatives in lieu of the new regs and I am already seeing underprocessed fatty breast tissue. Does anyone have a longer processing time for their breast (fatty) specimens they would be willing to share with me? Also, does anyone have a dedicated pneumatic tube system for your OR directly to histology for small specimens? Our facility is looking into this and I would appreciate any feedback!! Thanks ahead of time for any help in this area..I love this valuable access to my fellow histology lab workers!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From tim.morken <@t> thermofisher.com Mon Jan 15 10:33:00 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Jan 15 10:33:13 2007 Subject: [Histonet] TEM for paraffin embedded tissue section?? In-Reply-To: Message-ID: Ul Soo Choi, Here is a method perfect for your need to see neurosecretory granules. You can skip all the reprocessing from paraffin to EM resin by using 1 percent osmium in xylene to deparaffinize. This was pioneered by Kai Chien of Cedars-Sinai in Los Angeles. 1) Cut paraffin tissue in 1mm cubes. 2) deparaffinize in 1 percent osmium in xylene for several changes. 3) Clear in pure xylene 4) Infiltrate in a xylene/resin mix for several changes. 5) Infiltrate in pure resin 6) embed This will save hours and many of those tedious solution changes. The morphology isn't the great (or even good!) compared to standard EM methods, but it is useful for distinguishing lymphomas from carcinomas (lack of or presence of desmosomes), finding neurosecretory granuals in carcinoids and pre-melanosomes in melanomas. It is really only useful as a last attempt to get information after the specimen was wrongly submitted and all other tests fail to give answers. Reference: Chien, K, R.L. Van de Velde, R.C. Heusser, 1982, A one-step method for re-embedding paraffin-embedded specimens for electron microscopey, p.356-357, Proceedings fo the Electron Microscopy Society of America, 40th Annual Meeting, San Francisco Press, Inc, San Francisco, CA. Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Choi Ul Soo Sent: Sunday, January 14, 2007 9:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TEM for paraffin embedded tissue section?? Hello Dear Histonetters! would anyone there tell me the story about using paraffin embedded tissue section for TEM analysis? I have seen several reports reading that they used paraffin embedded tissues for transmisison electron microscopy, but our technician is not familiar to this method. Would the results be consistent regardless of the objects of interest, fixation or tissues? I'd like to get findings indicative of neuroendocrine origins from a canine lung mass fixed in formalin, and paraffin embedded tisse section. Please share your experiences and give me advice. Any inputs would be very much appreciated. Ul Soo Choi DVM, PhD VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742 Tel. 82-02-880-8688 Mobile. 82-016-9228-8634 Fax. 82-02-880-8662 _________________________________________________________________ ?? ?? ?? ??? ?????? MSN ???? ?????. http://fortune.msn.co.kr/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Mon Jan 15 10:42:05 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Mon Jan 15 10:43:19 2007 Subject: [Histonet] keys for cancers References: Message-ID: can anyone recommend a good reference for keying out pathologies such as cancers?? VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim Sent: Mon 1/15/2007 10:33 AM To: Choi Ul Soo Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TEM for paraffin embedded tissue section?? Ul Soo Choi, Here is a method perfect for your need to see neurosecretory granules. You can skip all the reprocessing from paraffin to EM resin by using 1 percent osmium in xylene to deparaffinize. This was pioneered by Kai Chien of Cedars-Sinai in Los Angeles. 1) Cut paraffin tissue in 1mm cubes. 2) deparaffinize in 1 percent osmium in xylene for several changes. 3) Clear in pure xylene 4) Infiltrate in a xylene/resin mix for several changes. 5) Infiltrate in pure resin 6) embed This will save hours and many of those tedious solution changes. The morphology isn't the great (or even good!) compared to standard EM methods, but it is useful for distinguishing lymphomas from carcinomas (lack of or presence of desmosomes), finding neurosecretory granuals in carcinoids and pre-melanosomes in melanomas. It is really only useful as a last attempt to get information after the specimen was wrongly submitted and all other tests fail to give answers. Reference: Chien, K, R.L. Van de Velde, R.C. Heusser, 1982, A one-step method for re-embedding paraffin-embedded specimens for electron microscopey, p.356-357, Proceedings fo the Electron Microscopy Society of America, 40th Annual Meeting, San Francisco Press, Inc, San Francisco, CA. Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Choi Ul Soo Sent: Sunday, January 14, 2007 9:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TEM for paraffin embedded tissue section?? Hello Dear Histonetters! would anyone there tell me the story about using paraffin embedded tissue section for TEM analysis? I have seen several reports reading that they used paraffin embedded tissues for transmisison electron microscopy, but our technician is not familiar to this method. Would the results be consistent regardless of the objects of interest, fixation or tissues? I'd like to get findings indicative of neuroendocrine origins from a canine lung mass fixed in formalin, and paraffin embedded tisse section. Please share your experiences and give me advice. Any inputs would be very much appreciated. Ul Soo Choi DVM, PhD VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742 Tel. 82-02-880-8688 Mobile. 82-016-9228-8634 Fax. 82-02-880-8662 _________________________________________________________________ ?? ?? ?? ??? ?????? MSN ???? ?????. http://fortune.msn.co.kr/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Jan 15 11:00:12 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jan 15 11:00:56 2007 Subject: [Histonet] guidelines In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2703640916@hpes1.HealthPartners.int> Message-ID: <006c01c738c6$9fdba300$6501a8c0@Patsy> Dorothy et al, One reason for the new Her2 guidelines from CAP is that there has been a discrepancy in results when samples are not adequately fixed in formalin and/or alcohol fixed. If the sample is not adequately cross linked by formalin fixation before it is processed, alcohols can damage or destroy the Her2 protein making it unavailable for detection. Your best bet is to fix the sample for 24 hrs. in formalin, then it will be protected from processing and can be retrieved but at least it is still there. I know, who has time to fix for 24hrs., well an approach to that could be that you take a piece of the sample and process it quickly for the H&E, while leaving another piece to fix for 24hrs., by the time the H&E is read and Her2 ordered your sample will be properly fixed, will withstand paraffin processing, then you do the IHC on the fixed piece. Requiring a minimum of 6 hrs fixation in formalin is how CAP is starting to address this problem. I expect that I am opening up a can of worms here but I think this is what this is about. Studies have been done in labs using alcohol fixatives and/or inadequate formalin fixation that show a drop in up to 30% of positive Her2 cases. That should be telling. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, January 15, 2007 9:00 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] guidelines With the new Her2 guidelines from CAP, how is everyone planning on handling not using alcoholic fixation of fatty breast tissue? We have been using Davidson's on mastectomy and lumpectomy specimens (not needle bx.s) and I have a dedicated processor with the first two stations of an alcoholic formalin fixative. My pathologists want these breast specimens to not be placed in these type of fixatives in lieu of the new regs and I am already seeing underprocessed fatty breast tissue. Does anyone have a longer processing time for their breast (fatty) specimens they would be willing to share with me? Also, does anyone have a dedicated pneumatic tube system for your OR directly to histology for small specimens? Our facility is looking into this and I would appreciate any feedback!! Thanks ahead of time for any help in this area..I love this valuable access to my fellow histology lab workers!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Mon Jan 15 11:56:01 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Jan 15 11:54:06 2007 Subject: [Histonet] guidelines In-Reply-To: <006c01c738c6$9fdba300$6501a8c0@Patsy> Message-ID: You can use an alternative fixative if you would like. This is from APPENDIX E Tissue Handling Requirement and Control Materials "Any alteration of standard conditions, such as use of alternative fixatives, microwave fixation, or alternative processing methods, must be validated against standard methods of testing before a test routinely using these conditions is offered in a laboratory. Validation must consist of testing of the same samples with the alternative fixative buffered formalin using the same HER2 testing method to demonstrate concordance of the result." http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, January 15, 2007 12:00 PM To: 'Webb, Dorothy L'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] guidelines Dorothy et al, One reason for the new Her2 guidelines from CAP is that there has been a discrepancy in results when samples are not adequately fixed in formalin and/or alcohol fixed. If the sample is not adequately cross linked by formalin fixation before it is processed, alcohols can damage or destroy the Her2 protein making it unavailable for detection. Your best bet is to fix the sample for 24 hrs. in formalin, then it will be protected from processing and can be retrieved but at least it is still there. I know, who has time to fix for 24hrs., well an approach to that could be that you take a piece of the sample and process it quickly for the H&E, while leaving another piece to fix for 24hrs., by the time the H&E is read and Her2 ordered your sample will be properly fixed, will withstand paraffin processing, then you do the IHC on the fixed piece. Requiring a minimum of 6 hrs fixation in formalin is how CAP is starting to address this problem. I expect that I am opening up a can of worms here but I think this is what this is about. Studies have been done in labs using alcohol fixatives and/or inadequate formalin fixation that show a drop in up to 30% of positive Her2 cases. That should be telling. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, January 15, 2007 9:00 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] guidelines With the new Her2 guidelines from CAP, how is everyone planning on handling not using alcoholic fixation of fatty breast tissue? We have been using Davidson's on mastectomy and lumpectomy specimens (not needle bx.s) and I have a dedicated processor with the first two stations of an alcoholic formalin fixative. My pathologists want these breast specimens to not be placed in these type of fixatives in lieu of the new regs and I am already seeing underprocessed fatty breast tissue. Does anyone have a longer processing time for their breast (fatty) specimens they would be willing to share with me? Also, does anyone have a dedicated pneumatic tube system for your OR directly to histology for small specimens? Our facility is looking into this and I would appreciate any feedback!! Thanks ahead of time for any help in this area..I love this valuable access to my fellow histology lab workers!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Mon Jan 15 12:16:41 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Jan 15 12:19:07 2007 Subject: [Histonet] guidelines Message-ID: <29BE166A2CF48D459853F8EC57CD37E87E9751@EXCHANGECLUSTER.yumaregional.local> The question I have is what do you do when you have to start moving results times back and the clinicians are already breathing down your neck for the DX. I like the idea that you take a piece of tissue and process it ahead of the rest this solves the issue of getting a diagnosis, or you could even do a frozen section (if possible). Has anyone looked at microwave processing as the answer to this?? Has the FDA approved breast studies on microwave processed tissue? I note the the current quote that was given states, as long as it is in occordance with the same result, that it is ok, does not cut it. What if you are a lab that is sending out blocks to have this study done and you are not fixing the specimen adequatley?? What if you do not do the studies but do the image analysis on the HER2 block?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, January 15, 2007 10:56 AM To: 'Patsy Ruegg'; 'Webb, Dorothy L'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] guidelines You can use an alternative fixative if you would like. This is from APPENDIX E Tissue Handling Requirement and Control Materials "Any alteration of standard conditions, such as use of alternative fixatives, microwave fixation, or alternative processing methods, must be validated against standard methods of testing before a test routinely using these conditions is offered in a laboratory. Validation must consist of testing of the same samples with the alternative fixative buffered formalin using the same HER2 testing method to demonstrate concordance of the result." http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, January 15, 2007 12:00 PM To: 'Webb, Dorothy L'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] guidelines Dorothy et al, One reason for the new Her2 guidelines from CAP is that there has been a discrepancy in results when samples are not adequately fixed in formalin and/or alcohol fixed. If the sample is not adequately cross linked by formalin fixation before it is processed, alcohols can damage or destroy the Her2 protein making it unavailable for detection. Your best bet is to fix the sample for 24 hrs. in formalin, then it will be protected from processing and can be retrieved but at least it is still there. I know, who has time to fix for 24hrs., well an approach to that could be that you take a piece of the sample and process it quickly for the H&E, while leaving another piece to fix for 24hrs., by the time the H&E is read and Her2 ordered your sample will be properly fixed, will withstand paraffin processing, then you do the IHC on the fixed piece. Requiring a minimum of 6 hrs fixation in formalin is how CAP is starting to address this problem. I expect that I am opening up a can of worms here but I think this is what this is about. Studies have been done in labs using alcohol fixatives and/or inadequate formalin fixation that show a drop in up to 30% of positive Her2 cases. That should be telling. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, January 15, 2007 9:00 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] guidelines With the new Her2 guidelines from CAP, how is everyone planning on handling not using alcoholic fixation of fatty breast tissue? We have been using Davidson's on mastectomy and lumpectomy specimens (not needle bx.s) and I have a dedicated processor with the first two stations of an alcoholic formalin fixative. My pathologists want these breast specimens to not be placed in these type of fixatives in lieu of the new regs and I am already seeing underprocessed fatty breast tissue. Does anyone have a longer processing time for their breast (fatty) specimens they would be willing to share with me? Also, does anyone have a dedicated pneumatic tube system for your OR directly to histology for small specimens? Our facility is looking into this and I would appreciate any feedback!! Thanks ahead of time for any help in this area..I love this valuable access to my fellow histology lab workers!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From chengkaz <@t> uci.edu Mon Jan 15 12:47:01 2007 From: chengkaz <@t> uci.edu (Chengkang ZHANG) Date: Mon Jan 15 12:49:20 2007 Subject: [Histonet] Re: BrdU staining Message-ID: <45ABCC25.30003@uci.edu> Hi Stephen, I assume that OE means Olfactory Epithelium. To eliminate the autofluorescence, you can try to incubate your sections in 5mM CuSO4 (50mM NH4OAc, pH5.0), after last step of your immunofluorescence staining, for 30 min to reduce lipofuscin-like autofluorescence, then wash in ddH2O briefly and in PBS for 5 min. This helps a lot in my hands. You can refer this paper for further reference. Stephen A. Schnell, William A. Staines and Martin W. Wessendorf. "Reduction of Lipofuscin-like Autofluorescence in Fluorescently Labeled Tissue", The journal of Histochemistry and Cytechemistry, 47(6), 719-730, 1999. Good luck Chengkang > Message: 1 > Date: Sun, 14 Jan 2007 13:37:04 -0600 > From: "Stephen Clark" > Subject: [Histonet] DAB brdU protocol > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have a protocol for DAB staining, specifically for mouse OE sections at about 10 or 18um using brdU? I had been using fluorescent staining procedures, but i wanted to try something non-flourescent to elimate any problems with autoflourescence. I have tried it a few times already with a rough protocol supplied by a colleague with little success. > -- ================================== Chengkang Zhang Ph.D. Room 357, MedSurge II 19182 Jamboree Rd, Department of Pharmacology University of California, Irvine Irvine, CA 92697-4625 Email: chengkaz@uci.edu Tel: (949)-824-1902 (lab) Fax: (949)-824-4855 ================================== From godsgalnow <@t> aol.com Mon Jan 15 14:10:43 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jan 15 14:11:18 2007 Subject: [Histonet] job reqs In-Reply-To: <398f02c20701130902q61277904o5ea1170ecc58ec12@mail.gmail.com> References: <398f02c20701130902q61277904o5ea1170ecc58ec12@mail.gmail.com> Message-ID: <8C906F85F00E09C-1E4-7CB6@FWM-D32.sysops.aol.com> Yes--you must have a license. Now, they will give a temporary license while you wait for your permanent. But, from my experience, it is not very difficult to get your license, if you meet all the qualifications and get everything to them in a timely manner. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: bayoubelle311@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sat, 13 Jan 2007 12:02 PM Subject: [Histonet] job reqs Question... in the state of Florida is it necessary to hold a license before you can qualify for a histo position? I currently have a bachelor degree plus some graduate work and some non-clinical histopath experience _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From godsgalnow <@t> aol.com Mon Jan 15 14:12:30 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jan 15 14:12:50 2007 Subject: [Histonet] job reqs In-Reply-To: <20070113180519.86439.qmail@web61216.mail.yahoo.com> Message-ID: <8C906F89EDFEFFE-1E4-7CC9@FWM-D32.sysops.aol.com> If you already have a national certification, like ASCP, you do not have to take another test. Florida excepts ASCP (and uses them to give their own test). Roxanne Soto HT(ASCP)QIHC Physicians RightPath Tampa, Florida -----Original Message----- From: rjbuesa@yahoo.com To: bayoubelle311@gmail.com; histonet@lists.utsouthwestern.edu Sent: Sat, 13 Jan 2007 1:05 PM Subject: Re: [Histonet] job reqs Hiring in Florida requires a license already issued OR if you have applied for it (if you qualify) while waiting to take the examination (provided that you pass). Permanent hiring is conditioned to passing the examination. The bachelor degree by itself does not qualify to work as a histotech. Ren? J. Missy wrote: Question... in the state of Florida is it necessary to hold a license before you can qualify for a histo position? I currently have a bachelor degree plus some graduate work and some non-clinical histopath experience _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From heupel <@t> zeiss.de Mon Jan 15 15:03:13 2007 From: heupel <@t> zeiss.de (Heupel, Thorsten) Date: Mon Jan 15 15:00:54 2007 Subject: [Histonet] Dr. Thorsten Heupel ist =?iso-8859-1?q?au=DFer_Haus_/_Dr=2E_Thors?= =?iso-8859-1?q?ten_Heupel_is_out_of_office?= Message-ID: Ich werde ab 15.01.2007 nicht im B?ro sein. Ich kehre zur?ck am 29.01.2007. Ich werde Ihre Nachrichten nach meiner R?ckkehr beantworten. Mit freundlichen Gr??en Thorsten Heupel I am out of the office from 01/15/2007 until 01/29/2007 I will respond to your message after my return. Best regards, Thorsten Heupel ________________________________________ Carl Zeiss MicroImaging GmbH Abteilung MI-VS / Department MI-VS Applikationsspezialist / Application Specialist D r. T h o r s t e n H e u p e l Telefon/Phone: +49 3641 64-2677 Fax: +49 3641 64-3144 mailto: heupel@zeiss.de http://www.zeiss.de/micro From Robert.Fauck <@t> ccdhb.org.nz Mon Jan 15 15:03:46 2007 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck) Date: Mon Jan 15 15:04:10 2007 Subject: [Histonet] Transport Media for Skin Fluorescence Message-ID: Hallo Everybody, Just like to know if this receipe is wright for Transport Media for Skin Fluorescence. Ammonium Sulphate 412.31gms N-Ethylmaleimide 0.65gm Magnesium Sulphate 1.23gm Potassium Citrate 8.11gms Destilled Water 1 Liter pH 7.0 The amount of 412.31 gms of Amm.Sulphate looks an awfull lot to me for 1 Liter, can anybody confirm if the receipe is correct and in which book I can find a reference for it?! Thanking you in advance for your help. Cheers, Robert Fauck Wellington Hospital New Zealand This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) No Viruses were detected in this message. HealthIntelligence eMail Filter Service From tkngflght <@t> yahoo.com Mon Jan 15 16:05:35 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Mon Jan 15 16:07:33 2007 Subject: [Histonet] job reqs In-Reply-To: <8C906F85F00E09C-1E4-7CB6@FWM-D32.sysops.aol.com> References: <398f02c20701130902q61277904o5ea1170ecc58ec12@mail.gmail.com> <8C906F85F00E09C-1E4-7CB6@FWM-D32.sysops.aol.com> Message-ID: <002601c738f1$4913b2d0$6401a8c0@FSDESKTOP> A note on the FL temp license-- Please correct me if I'm wrong but this is how it was explained to me the last time I called in to the Florida Dept of Health--You only qualify for a temporary license if you're trying to go through the experience/education pathways. If you have your ASCP, you are almost guaranteed a license but they no longer give a temporary for those with ASCP registry. Advice to those who go for their Florida certs: Don't send the application until it is complete and as close to perfect on your first try. There are two lovely ladies who staff this office. If something is missing they send a letter and you go back to the bottom of the stack. Make their lives easier by getting everything in at once and you can save yourself a lot of waiting. Cheryl Kerry Full Staff Inc. 800.756.3309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, January 15, 2007 2:11 PM To: bayoubelle311@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] job reqs Yes--you must have a license. Now, they will give a temporary license while you wait for your permanent. But, from my experience, it is not very difficult to get your license, if you meet all the qualifications and get everything to them in a timely manner. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: bayoubelle311@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sat, 13 Jan 2007 12:02 PM Subject: [Histonet] job reqs Question... in the state of Florida is it necessary to hold a license before you can qualify for a histo position? I currently have a bachelor degree plus some graduate work and some non-clinical histopath experience _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Steven2146 <@t> aol.com Mon Jan 15 16:33:42 2007 From: Steven2146 <@t> aol.com (Steven2146@aol.com) Date: Mon Jan 15 16:34:44 2007 Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 22 Message-ID: Hi Derek, I've been a Mohs tech and histotech for many years and have set up path labs in physicians offices. For me, the gold standard is the VIP processor and the Leica cryostats. The only company I've ever dealt with for these instruments is Belair Scientific, out of New Jersey. They also just happen to have the exclusive rights to sell the Leica 1510S Cryostat...an absolute gem of an instrument. They also sell refurbished/reconditioned equipment...the VIP being one of them and to my knowledge, the only company that gives a full one year warranty on every instrument they sell. Call them, I think you'll find them to be a pleasure to deal with. Steven Lee HT ASCP From tkngflght <@t> yahoo.com Mon Jan 15 16:41:12 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Mon Jan 15 16:41:25 2007 Subject: [Histonet] Link to Florida Dept of Health New Licensee Services In-Reply-To: <002601c738f1$4913b2d0$6401a8c0@FSDESKTOP> References: <398f02c20701130902q61277904o5ea1170ecc58ec12@mail.gmail.com><8C906F85F00E09C-1E4-7CB6@FWM-D32.sysops.aol.com> <002601c738f1$4913b2d0$6401a8c0@FSDESKTOP> Message-ID: <004101c738f6$4282ff20$6401a8c0@FSDESKTOP> http://ww2.doh.state.fl.us/mqaservices/PractitionerServices.asp?Index=4 Click (or Alt. + click) on the link..read on! Cheryl From SB_Cohen <@t> fccc.edu Mon Jan 15 17:42:56 2007 From: SB_Cohen <@t> fccc.edu (Cohen, Sherene B.) Date: Mon Jan 15 17:50:18 2007 Subject: [Histonet] Job Opportunity Message-ID: <670B8345AF238F40910FEC4CA7D4B7D2F9E912@exchserver.fccc.edu> Fox Chase Cancer Center in Philadelphia, PA has an opening for a full time histotechnician. Hours are Monday to Friday, no nights, no weekends. If interested, please e-mail resume to: sherene.cohen@fccc.edu. From suetrant <@t> telus.net Mon Jan 15 21:17:06 2007 From: suetrant <@t> telus.net (Sue Trant) Date: Mon Jan 15 21:17:48 2007 Subject: [Histonet] paraffin embedded tissue for EM Message-ID: <001301c7391c$ccf62760$0200a8c0@ROGER> Hi all I also have taken paraffin-B5 fixed embedded blocks back to a state that I can use for electron microscopy. I have had very good results, but it is a long process of approximately 8 hours. You need to use 2-3 changes of xylenes, followed by graded alcohols starting with 100 % and tapering to 25%. This is followed by distilled water. You can then post fix in a 3.5% gluteraldehyde (fixed for 2 days), and proceed through a regime of your regular fixation with an OsO4 solution, etc. Sue Trant EM Histotechnologist-VIHA -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.16.12/628 - Release Date: 2007-01-15 From sonya.martin <@t> soton.ac.uk Tue Jan 16 04:04:23 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Jan 16 04:05:13 2007 Subject: [Histonet] Tumour endothelium - continued Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34DB@ISS-CL-EX-V1.soton.ac.uk> Thanks to everyone for your replies; just one more problem - its mouse endothelium I want to look at rather than human. I think the problem with the tomato lectin is cross-readtivity with epithelial cells. I have found an anti-mouse Factor VIII antibody from Abcam but its quite expensive.....budgets!! Most of the tissues are FFPE and I cant find an anti-mouse CD31 antibdy reported to work realiably in paraffins. Any more suggestions? Cheers Sonya From boor <@t> email.cz Tue Jan 16 08:06:36 2007 From: boor <@t> email.cz (Boor Peter) Date: Tue Jan 16 08:06:58 2007 Subject: [Histonet] DAB black/brown Message-ID: <006301c73977$88e99790$ae0b8286@ZION> Can anybody help? For our IH we're using DAB with Ni chloride to get black color (especially for double stains with AEC). In the last period however we get also brown coloured stainings and even in one run we get some slides with brown some with black color (very bad for our double stains). We used new DAB, made fresh Ni chloride solution and H2O2 but nothing helped till now...we now even got some slides stained half black half brown... This is especially bad for our double stainings (it's very hard to distinguish brown from red). Where could be the problem? Thanks a lot for any suggestions! Peter Boor Peter Boor University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. 30; 52074 Aachen; Germany Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany Mob.: 0049 (0) 177 2345 163 Lab: 0049 (0) 241 80 89670 Fax: 0049 (0) 241 80 82446 ICQ Nr.: 259 368 084 From sbreeden <@t> nmda.nmsu.edu Tue Jan 16 08:32:35 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jan 16 08:36:39 2007 Subject: [Histonet] New Mexico Society for Histology Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB40E@nmdamailsvr.nmda.ad.nmsu.edu> The 2007 Annual Meeting for the NM Society for Histology will be held on Saturday, May 12th in Albuquerque. Planning for the meeting is underway and meeting location, schedule, etc. will be announced. This year marks the 15th anniversary of the formation of NMSH and we're celebrating! Vendors who would like to take part in this event are encouraged to contact me at nmhisto@aol.com so you can be sent the meeting information. Potential speakers who have a burning need to take a little vacation in New Mexico while addressing our Society are also encouraged to contact me. The 2006 Annual Meeting was a wonderful success after a 4-year hiatus, with 30 techs attending and 21 vendors present or represented! We're out to match and exceed those numbers. Mark your calendars and email me! Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From kimtournear <@t> yahoo.com Tue Jan 16 08:36:46 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Jan 16 08:36:55 2007 Subject: [Histonet] square tissue freezing chucks Message-ID: <59318.57283.qm@web37712.mail.mud.yahoo.com> Hi, I'm in need of the square, 25mm freezing discs (chucks) used on the ThermShandon cryostat. I can find the 45mm, but they are too big. Can anyone tell me who is selling them. I've checked the catalogs from Fisher, SurgiPath, IMEB, Richard Allan, but I don't have a Shandon catalog. Are the in there? Any info would be appreciated. Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ Kim ~~ Don't be afraid your life will end, be afraid it will never begin ~~ --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From rjbuesa <@t> yahoo.com Tue Jan 16 08:43:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 16 08:47:29 2007 Subject: [Histonet] DAB black/brown In-Reply-To: <006301c73977$88e99790$ae0b8286@ZION> Message-ID: <9312.87758.qm@web61218.mail.yahoo.com> Peter: Just bear with me if I review with you my protocol: For a second chromogen (double staining) I used: DAB --- 6 mg, dissolved in 10 mL of PBS to which I added 160?L of 3% H2O2 To this mixture I added 120?L of a 16,2% aq. solution of nickel sulfate hexahydrated. I let the solution to act for 6 minutes. Results = BLUE-PURPLISH, but I never got to a BLACK counterstain, which makes me think that you are using too much NiSO4.6H2O In all reality, I think that blue versus brown is a better contrast for a double stain. If you are doing it exactly this way I do not know what to tell you, but I think that if in the same slide you are getting different hues in different areas it may be due to unequal distribution of the solution or an uneven solution distributed evenly. Hope this will help you! Ren? J. Boor Peter wrote: Can anybody help? For our IH we're using DAB with Ni chloride to get black color (especially for double stains with AEC). In the last period however we get also brown coloured stainings and even in one run we get some slides with brown some with black color (very bad for our double stains). We used new DAB, made fresh Ni chloride solution and H2O2 but nothing helped till now...we now even got some slides stained half black half brown... This is especially bad for our double stainings (it's very hard to distinguish brown from red). Where could be the problem? Thanks a lot for any suggestions! Peter Boor Peter Boor University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. 30; 52074 Aachen; Germany Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany Mob.: 0049 (0) 177 2345 163 Lab: 0049 (0) 241 80 89670 Fax: 0049 (0) 241 80 82446 ICQ Nr.: 259 368 084 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From EWURDAK <@t> CSBSJU.EDU Tue Jan 16 09:34:02 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Tue Jan 16 09:34:13 2007 Subject: [Histonet] Botanical stains Message-ID: Hello Histonetters, I would like to express my thanks to all who responded to my request for information about staining plant tissues. We shall be trying out some of the suggested protocols in our histology class. A happy New year to all of you. Elizabeth Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 From Jackie.O'Connor <@t> abbott.com Tue Jan 16 09:59:30 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jan 16 10:04:03 2007 Subject: [Histonet] Tumour endothelium - continued In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E34DB@ISS-CL-EX-V1.soton.ac.uk> Message-ID: Biocare Medical has a working CD31 for mouse FFPE tissues. "Martin S." Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2007 04:04 AM To cc Subject [Histonet] Tumour endothelium - continued Thanks to everyone for your replies; just one more problem - its mouse endothelium I want to look at rather than human. I think the problem with the tomato lectin is cross-readtivity with epithelial cells. I have found an anti-mouse Factor VIII antibody from Abcam but its quite expensive.....budgets!! Most of the tissues are FFPE and I cant find an anti-mouse CD31 antibdy reported to work realiably in paraffins. Any more suggestions? Cheers Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jan 16 10:17:42 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jan 16 10:19:35 2007 Subject: [Histonet] DAB black/brown In-Reply-To: <006301c73977$88e99790$ae0b8286@ZION> References: <006301c73977$88e99790$ae0b8286@ZION> Message-ID: <6.0.0.22.1.20070116090559.01ba03e0@gemini.msu.montana.edu> Chris van der loos teaches (he is the master of double IHC or any multiple staining) that using a blue and a red gives far better contrast along with better tinctorial quality. He also teachs that one antibody be detected with alkaline phosphatase (develop this color first) and the other with peroxidase although careful requenching of peroxidase from the first antibody staining can be done. AEC with Vector Blue is superb although the Vector blue is a bit less sensitive DAB or AEC, which means that the primary antibody titer with this chromogen may have to increase. AEC tends to be orange-red in color, and clashes with the brown of DAB which is what you observed. A better combination with your DAB would be DAKO's Permanent red which give rich reddish -pink tones that separates much better from the brown DAB for the contrast you need. The joy of Permanent red is the sensitivity, maybe even more so than AEC so retitering the antibody may be needed. You would not need to enhance the DAB to black with Permanent red. Good luck Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 07:06 AM 1/16/2007, you wrote: >Can anybody help? > >For our IH we're using DAB with Ni chloride to get black color (especially >for double stains with AEC). >In the last period however we get also brown coloured stainings and even in >one run we get some slides with brown some with black color (very bad for >our double stains). >We used new DAB, made fresh Ni chloride solution and H2O2 but nothing helped >till now...we now even got some slides stained half black half brown... >This is especially bad for our double stainings (it's very hard to >distinguish brown from red). > >Where could be the problem? > >Thanks a lot for any suggestions! > >Peter Boor > > >Peter Boor > >University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. >30; 52074 Aachen; Germany > >Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany > >Mob.: 0049 (0) 177 2345 163 > >Lab: 0049 (0) 241 80 89670 > >Fax: 0049 (0) 241 80 82446 > >ICQ Nr.: 259 368 084 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Jan 16 10:31:30 2007 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Jan 16 10:32:35 2007 Subject: [Histonet] RE: Histonet Digest, Vol 38, Issue 22 Message-ID: Dear Stephen Clark: If by OE, you mean olfactory epithelium, we stain that frequently for BrdU. My first question is, are you decalcifying, and with what? That will critically affect the success of your staining. We have found that you can use either EDTA or formic acid decalcification, but we could NOT get BrdU staining after using other treatments, like the rapid (probably sulfuric acid) decalcifiers. But if you had success with fluorescence, you should have it with DAB. Email me privately and I will be happy to send you some methods. Sarah Pixley, Sarah.pixley@uc.edu Message: 1 Date: Sun, 14 Jan 2007 13:37:04 -0600 From: "Stephen Clark" Subject: [Histonet] DAB brdU protocol To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone have a protocol for DAB staining, specifically for mouse OE sections at about 10 or 18um using brdU? I had been using fluorescent staining procedures, but i wanted to try something non-flourescent to elimate any problems with autoflourescence. I have tried it a few times already with a rough protocol supplied by a colleague with little success. From tp2 <@t> medicine.wisc.edu Tue Jan 16 10:41:39 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Jan 16 10:42:28 2007 Subject: [Histonet] DAB black/brown Message-ID: <45ACABE3020000DF00004303@gwmail.medicine.wisc.edu> If contrast is what you seek, you could always do a DAB, a Vector VIP (very intense purple), and a methyl green counter stain. There will be no doubt as to what is what. Tom Pier >>> Gayle Callis 01/16/07 10:17 AM >>> Chris van der loos teaches (he is the master of double IHC or any multiple staining) that using a blue and a red gives far better contrast along with better tinctorial quality. He also teachs that one antibody be detected with alkaline phosphatase (develop this color first) and the other with peroxidase although careful requenching of peroxidase from the first antibody staining can be done. AEC with Vector Blue is superb although the Vector blue is a bit less sensitive DAB or AEC, which means that the primary antibody titer with this chromogen may have to increase. AEC tends to be orange-red in color, and clashes with the brown of DAB which is what you observed. A better combination with your DAB would be DAKO's Permanent red which give rich reddish -pink tones that separates much better from the brown DAB for the contrast you need. The joy of Permanent red is the sensitivity, maybe even more so than AEC so retitering the antibody may be needed. You would not need to enhance the DAB to black with Permanent red. Good luck Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 07:06 AM 1/16/2007, you wrote: >Can anybody help? > >For our IH we're using DAB with Ni chloride to get black color (especially >for double stains with AEC). >In the last period however we get also brown coloured stainings and even in >one run we get some slides with brown some with black color (very bad for >our double stains). >We used new DAB, made fresh Ni chloride solution and H2O2 but nothing helped >till now...we now even got some slides stained half black half brown... >This is especially bad for our double stainings (it's very hard to >distinguish brown from red). > >Where could be the problem? > >Thanks a lot for any suggestions! > >Peter Boor > > >Peter Boor > >University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. >30; 52074 Aachen; Germany > >Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany > >Mob.: 0049 (0) 177 2345 163 > >Lab: 0049 (0) 241 80 89670 > >Fax: 0049 (0) 241 80 82446 > >ICQ Nr.: 259 368 084 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Tue Jan 16 10:49:36 2007 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Tue Jan 16 10:48:36 2007 Subject: [Histonet] A question to the Histonet Webmaster Message-ID: <5784D843593D874C93E9BADCB87342AB027D99CE@tpiserver03.Coretech-holdings.com> The following was erroneously sent to histonet.org, the site for pictures to be posted by histonetters. I will reply with instructions for getting on the histonet. Meanwhile, does anyone have an answer? textfield = MVaha@aol.com textfield2 = Marianna textfield3 = Thank God I hbe found you. I am looking for certification in California. I have not worked for 10 years as a Histo Tech, since I moved to Maui. At that time there were no labs open all the work was being sent to Oahu. Now I have returned to California and desire to work but find that it is not easy without certification. I have 15 years experience before going to Hawaii. When I last worked I did not need my certification. Please tell me where I can get my certification on line or a local college. Sincerely Mary Vaha PS I have worked with all levels of Histology using sliding microtome (nitrocellulose,rotary, cryostate and all embedding media ... because my work was primarily in research) Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com From gcallis <@t> montana.edu Tue Jan 16 10:51:52 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jan 16 10:51:56 2007 Subject: [Histonet] Luxol Fast Blue In-Reply-To: References: Message-ID: <6.0.0.22.1.20070116092644.01bd6eb0@gemini.msu.montana.edu> Thomas, Fine tuning the luxol fast blue (LFB) can be touchy sometimes. We use the classic Kluver Barrera method LFB overnight in 60C waterbath, Brief 95% ethanol rinse to remove LFB Distilled water Differentiate in 0.05 to 0.08% lithium carbonate approximately 10 to 20 dips, then go to 70% and carefully watch the LFB stream away from the section. Beware, this is FAST!! It is important to NOT dip in 70% ethanol too long. Rinse really well with distilled water (use several changes) and use the microscope to see if myelin is well distinguished or the white matter from gray matter. If you need a bit more blue removed, then repeat the lithium carbonate and 70% alcohol step, very, very carefully. We have found brain differentiates a bit faster than the spinal cord and your sections are a bit thicker although some do 10um sections for brain/spinal cord. You may be overdifferentiating as you want to see the myelin (this tends to look like strands of blue tissue). After rinsing thoroughly with distilled water, do the PAS stain - we like 15 min in 1% periodic acid then 20 minutes in PAS, rinse with running tap water for 10 min. There are other variations on time with PAS. When you are finished, start your dehydration in 95% alcohol then 100% and do these steps very quickly by dipping. We never go back to 70% after just LFB or PAS. By purple color, do you mean more of a gross observation of the section as this would seem normal.. Microscopically you see the blue myelinated areas with reddish pink PAS structures and not a purplish overall color. When we look at our sections macroscopically (no microscope) and with EAE virus murine model, we can still see areas of demyelination on edges of cord, but the overall color of LFB-PAS is a bit purplish. At 10:52 AM 1/12/2007, you wrote: >I'm trying to stain mouse spinal cord sections, (7 um), with Luxol Fast >Blue and periodic acid/Schiff and hematoxylin. In the literature I've >been reading, there are some great examples of a nice pink to red stain of >the grey matter, and a nice solid blue stain of the white matter. > >Mine look not so good, (purple), and I've been struggling for a while >with it. I've tried so many adjustments to the protocol that it would be >too long to list here. But I think the heart of my problem is with LFB, >differentiating the grey matter completely with out fading the >blue-stained white matter. Somebody please tell me the secret! > >I've experimented with the LFB alone, and believe that differentiating in >lithium carbonate, with or with out 70% EtOH, should clear the grey matter >of the stain, and leave the white matter blue or turquoise. But it only >clears it somewhat, and any further attempt to differentiate also quickly >fades the white matter. Any help is greatly appreciated. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From GoodwinD <@t> pahosp.com Tue Jan 16 11:21:16 2007 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Tue Jan 16 11:21:28 2007 Subject: [Histonet] Hacker MARS tissue processor Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8504@uphsmbx2.UPHS.PENNHEALTH.PRV> Greetings, Histonet. Anyone using the Hacker MARS microwave tissue processor? Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From STapper <@t> slhduluth.com Tue Jan 16 11:55:52 2007 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Tue Jan 16 11:56:03 2007 Subject: [Histonet] frozen section fixative Message-ID: What are people using to fix their frozen sections prior to staining with H&E? Thanks! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN 55805 From boor <@t> email.cz Tue Jan 16 12:00:30 2007 From: boor <@t> email.cz (Boor Peter) Date: Tue Jan 16 12:04:35 2007 Subject: [Histonet] VEGFR1 and 2 in rat Message-ID: <008001c73998$35dd3450$ae0b8286@ZION> Hi! Can anyone recommend an antibody for VEGFR 1 and 2 that works well in rat? We only tested anti-human polyclonals (R&D) with various Ag retrieval protocols with no result. Also, there are Ab against human phosphorylated VEGFR that should be apliable for IH (Calbiochem), do they work also in rat tissue? Thanks a lot for any information! Peter Boor Peter Boor University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. 30; 52074 Aachen; Germany Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany Mob.: 0049 (0) 177 2345 163 Lab: 0049 (0) 241 80 89670 Fax: 0049 (0) 241 80 82446 ICQ Nr.: 259 368 084 From godsgalnow <@t> aol.com Tue Jan 16 12:05:08 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Jan 16 12:05:28 2007 Subject: [Histonet] Hacker MARS tissue processor In-Reply-To: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8504@uphsmbx2.UPHS.PENNHEALTH.PRV> References: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8504@uphsmbx2.UPHS.PENNHEALTH.PRV> Message-ID: <8C907AFFDDA1F96-A40-13F1@WEBMAIL-RA12.sysops.aol.com> I am not using it, but I hear it is great. I am trying to set up a demo. I would also be interested in any feedback. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: GoodwinD@pahosp.com To: histonet@lists.utsouthwestern.edu Cc: rsiderit@rwjuhh.edu Sent: Tue, 16 Jan 2007 12:21 PM Subject: [Histonet] Hacker MARS tissue processor Greetings, Histonet. Anyone using the Hacker MARS microwave tissue processor? Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From bhewlett <@t> cogeco.ca Tue Jan 16 12:05:50 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Jan 16 12:06:05 2007 Subject: [Histonet] frozen section fixative References: Message-ID: <002c01c73998$f51f2620$6500a8c0@mainbox> I always used FAA (70% ETOH containing 5% acetic acid and 4% formaldehyde). Fix QS for 30 -60 seconds and proceed with a progressive H&E. Bryan ----- Original Message ----- From: "Tapper, Sheila" To: Sent: Tuesday, January 16, 2007 12:55 PM Subject: [Histonet] frozen section fixative What are people using to fix their frozen sections prior to staining with H&E? Thanks! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN 55805 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boor <@t> email.cz Tue Jan 16 12:00:30 2007 From: boor <@t> email.cz (Boor Peter) Date: Tue Jan 16 12:07:59 2007 Subject: [Histonet] VEGFR1 and 2 in rat Message-ID: <008001c73998$35dd3450$ae0b8286@ZION> Hi! Can anyone recommend an antibody for VEGFR 1 and 2 that works well in rat? We only tested anti-human polyclonals (R&D) with various Ag retrieval protocols with no result. Also, there are Ab against human phosphorylated VEGFR that should be apliable for IH (Calbiochem), do they work also in rat tissue? Thanks a lot for any information! Peter Boor Peter Boor University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. 30; 52074 Aachen; Germany Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany Mob.: 0049 (0) 177 2345 163 Lab: 0049 (0) 241 80 89670 Fax: 0049 (0) 241 80 82446 ICQ Nr.: 259 368 084 From MTrego <@t> chesterriverhealth.org Tue Jan 16 12:10:45 2007 From: MTrego <@t> chesterriverhealth.org (Trego, Melanie) Date: Tue Jan 16 12:10:54 2007 Subject: [Histonet] frozen section fixative Message-ID: <05505FB3EF11404EAE54786F0B76B1725040B2@crhsmail.crhs.org> I found a great F/S fixative from American Master Tech Scientific. Have been using it for several years. It works quickly, does not contain formalin and smells a bit like chocolate. Now I rarely lose a section (mostly cartilage, in which case we use lysine treated slides). Mel Trego Chester River Hospital Center Chestertown, MD 21620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tapper, Sheila Sent: Tuesday, January 16, 2007 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen section fixative What are people using to fix their frozen sections prior to staining with H&E? Thanks! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN 55805 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From gcallis <@t> montana.edu Tue Jan 16 12:22:15 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jan 16 12:22:20 2007 Subject: [Histonet] frozen section fixative In-Reply-To: References: Message-ID: <6.0.0.22.1.20070116112135.01b83708@gemini.msu.montana.edu> neutral buffered formalin for best morphology, but have use 95% ethanol too. At 10:55 AM 1/16/2007, you wrote: >What are people using to fix their frozen sections prior to staining >with H&E? > > > >Thanks! > > > >Sheila Tapper HT(ASCP) > >St. Luke's Hospital > >Duluth, MN 55805 > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From sohail_e <@t> yahoo.com Tue Jan 16 12:18:50 2007 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Tue Jan 16 12:22:55 2007 Subject: [Histonet] Antibody bleaching chemicals Message-ID: <160005.17832.qm@web39512.mail.mud.yahoo.com> Hi I am intesrested in bleaching my slide which is already labelled for some antibodies. This is because i am intrested in using more then ten antibodies on the same slide. I would like to prefer some chemical way to bleach already stained antibodies *I have already tried bleaching by excitation wavelength and now interesed in comparing it with some chemical bleaching way. Please respond to slove the issue. Allis --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From BoozerKA <@t> ah.org Tue Jan 16 12:43:07 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Tue Jan 16 12:44:01 2007 Subject: [Histonet] HP Controls Message-ID: <45ACAC3B020000C0000022E8@ahgwrsvl.ah.org> Someone sent me two blocks - I need a return address to send AFB back. Thanks! Kathy From mtitford <@t> aol.com Tue Jan 16 12:58:54 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Tue Jan 16 12:59:16 2007 Subject: [Histonet] "Home made" IF transport media Message-ID: <8C907B780AA33B9-F18-1836@WEBMAIL-MA07.sysops.aol.com> Robert Fauck in New Zealand asks about "home brewed" immunofluorescence transport media. Dr Elias gave a simlier formula in the very first issue of the Journal of Histotechnology ("New method for shipment of renal biopsies", Elias. J.M., et al. J. Histotechnol 1: 15-16 1977) His was a two part solution of a buffer, and a working solution: Stock buffer 1M Potassium citrate buffer pH 7.0....2.5ml 0.1M Magnesium sulphate................5.0ml 0.1M N-ethyl maleimide....................5.0ml Distilled water..............................87.5ml (Adjust to pH 7.0 with 1M KOH) Working solution Ammonium sulphate (NH4)2SO4...................55 grams Stock buffer..............................................100 ml Hope this helps Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Luis.Chiriboga <@t> med.nyu.edu Tue Jan 16 13:09:27 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Tue Jan 16 13:03:33 2007 Subject: [Histonet] Microtome Accessory Question Message-ID: Does anyone know who manufactures/sells the collars that sit behind the chuck assembly. They are supposed to keep the chuck angle/plane from changing? This would be for a Reichert-Jung 2030 microtome? Thanks in advance Luis From portera <@t> msu.edu Tue Jan 16 13:06:30 2007 From: portera <@t> msu.edu (Amy Porter) Date: Tue Jan 16 13:04:41 2007 Subject: [Histonet] VEGFR1 and 2 in rat References: <008001c73998$35dd3450$ae0b8286@ZION> Message-ID: <001f01c739a1$6e09ea90$8e7a0923@histolab> I would check Santa Cruz www.scbt.com Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Boor Peter" To: Sent: Tuesday, January 16, 2007 1:00 PM Subject: [Histonet] VEGFR1 and 2 in rat > Hi! > > Can anyone recommend an antibody for VEGFR 1 and 2 that works well in rat? > We only tested anti-human polyclonals (R&D) with various Ag retrieval > protocols with no result. > Also, there are Ab against human phosphorylated VEGFR that should be > apliable for IH (Calbiochem), do they work also in rat tissue? > > Thanks a lot for any information! > > Peter Boor > > > Peter Boor > > University Clinic of Aachen, Dept. of Nephrology and Immunology, > Pauwelsstr. > 30; 52074 Aachen; Germany > > Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany > > Mob.: 0049 (0) 177 2345 163 > > Lab: 0049 (0) 241 80 89670 > > Fax: 0049 (0) 241 80 82446 > > ICQ Nr.: 259 368 084 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Tue Jan 16 13:01:14 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 16 13:05:38 2007 Subject: AW: [Histonet] frozen section fixative In-Reply-To: Message-ID: <000901c739a0$bc5cd6e0$c812a8c0@dielangs.at> 37-40% Formaldehyd Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tapper, Sheila Gesendet: Dienstag, 16. J?nner 2007 18:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] frozen section fixative What are people using to fix their frozen sections prior to staining with H&E? Thanks! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN 55805 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Tue Jan 16 13:18:18 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Jan 16 13:18:31 2007 Subject: [Histonet] frozen section fixative In-Reply-To: <000901c739a0$bc5cd6e0$c812a8c0@dielangs.at> References: <000901c739a0$bc5cd6e0$c812a8c0@dielangs.at> Message-ID: I use 10% neutral buffered formalin or 4% PFA for 10 minutes. Works well with paraffin quality results. >-----Urspr?ngliche Nachricht----- >Von: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tapper, >Sheila >Gesendet: Dienstag, 16. J?nner 2007 18:56 >An: histonet@lists.utsouthwestern.edu >Betreff: [Histonet] frozen section fixative > >What are people using to fix their frozen sections prior to staining >with H&E? > > > >Thanks! > > > >Sheila Tapper HT(ASCP) > >St. Luke's Hospital > >Duluth, MN 55805 > > -- From funderwood <@t> mcohio.org Tue Jan 16 13:24:30 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Tue Jan 16 13:24:55 2007 Subject: [Histonet] Microtome Accessory Question Message-ID: Call Leica Microsystems at 1-800-248-0123. I got a chuck for my 2030 from them. Fred >>> Luis Chiriboga 1/16/2007 2:09 PM >>> Does anyone know who manufactures/sells the collars that sit behind the chuck assembly. They are supposed to keep the chuck angle/plane from changing? This would be for a Reichert-Jung 2030 microtome? Thanks in advance Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jan 16 14:00:31 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 16 14:00:38 2007 Subject: [Histonet] A question to the Histonet Webmaster In-Reply-To: <5784D843593D874C93E9BADCB87342AB027D99CE@tpiserver03.Coretech-holdings.com> Message-ID: California does not require certification, but most facilities want ASCP certification. Please go to www.ascp.org to find out how to apply for certification. There are two routes for HT certification eligibility. Route 1 is graduation from a NAACLS accredited program. Route 2 is an associate degree (or equivalent, with 12 semester hours of biology and chemistry) and one year full time experience in a histology laboratory. The only NAACLs accredited program in California is here at Mt. San Antonio College. If you have access to a histology laboratory there are a couple of other options. Check out www.nsh.org for schools that offer alternative education opportunities. Jennifer MacDonald Mt. San Antonio College "Charles Scouten" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2007 08:49 AM To , cc Subject [Histonet] A question to the Histonet Webmaster The following was erroneously sent to histonet.org, the site for pictures to be posted by histonetters. I will reply with instructions for getting on the histonet. Meanwhile, does anyone have an answer? textfield = MVaha@aol.com textfield2 = Marianna textfield3 = Thank God I hbe found you. I am looking for certification in California. I have not worked for 10 years as a Histo Tech, since I moved to Maui. At that time there were no labs open all the work was being sent to Oahu. Now I have returned to California and desire to work but find that it is not easy without certification. I have 15 years experience before going to Hawaii. When I last worked I did not need my certification. Please tell me where I can get my certification on line or a local college. Sincerely Mary Vaha PS I have worked with all levels of Histology using sliding microtome (nitrocellulose,rotary, cryostate and all embedding media ... because my work was primarily in research) Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garret.t.miyamoto <@t> us.army.mil Tue Jan 16 14:14:21 2007 From: garret.t.miyamoto <@t> us.army.mil (garret.t.miyamoto@us.army.mil) Date: Tue Jan 16 14:14:33 2007 Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 23 In-Reply-To: <5j0m71$57apn7@mxoutdr1.us.army.mil> References: <5j0m71$57apn7@mxoutdr1.us.army.mil> Message-ID: Re: frozen section fixative Sheila, We just use 95% ethanol to fix the tissue prior to staining with H&E. Five dips should suffice. Garret ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Tuesday, January 16, 2007 8:10 am Subject: Histonet Digest, Vol 38, Issue 23 From eileen_dusek <@t> yahoo.com Tue Jan 16 14:20:52 2007 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Tue Jan 16 14:20:59 2007 Subject: [Histonet] Bacteria on the slides Message-ID: <406375.87375.qm@web50603.mail.yahoo.com> Hi Everyone, We are having a major problem with AFB, Giemsa and know Fe staining. On the slides its self, outside the tissue, we are getting AFB, Giemsa and Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are stained on a staining rack. Our stainer recently had the water filter changed. This is occurring with all the Techs. We use charged slides for all specials, Could this be in the paraffin? The charged slides? All suggestions are appreciated. Eileen Edward Hospital --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From ryakay <@t> shands.ufl.edu Tue Jan 16 14:28:59 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Tue Jan 16 14:30:20 2007 Subject: [Histonet] job opportunity Message-ID: Two full time histologist openings in the home of the Florida "fighting gators", beautiful Gainesville, Florida. One late evening position and one early morning position. If interested please call for more information. See below.... Kaye Kaye Ryan Histology Manager/Educational Coordinator Rocky Point Laboratories 265-0111, 72093 From liz <@t> premierlab.com Tue Jan 16 15:16:49 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jan 16 15:07:59 2007 Subject: [Histonet] Bacteria on the slides In-Reply-To: <406375.87375.qm@web50603.mail.yahoo.com> Message-ID: <000701c739b3$a2e3dd40$0d00a8c0@domain.Premier> Could be in your water bath, do you change and clean your waterbaths daily? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen dusek Sent: Tuesday, January 16, 2007 1:21 PM To: histonet Subject: [Histonet] Bacteria on the slides Hi Everyone, We are having a major problem with AFB, Giemsa and know Fe staining. On the slides its self, outside the tissue, we are getting AFB, Giemsa and Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are stained on a staining rack. Our stainer recently had the water filter changed. This is occurring with all the Techs. We use charged slides for all specials, Could this be in the paraffin? The charged slides? All suggestions are appreciated. Eileen Edward Hospital --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jan 16 15:25:43 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jan 16 15:25:46 2007 Subject: [Histonet] Bacteria on the slides In-Reply-To: <406375.87375.qm@web50603.mail.yahoo.com> References: <406375.87375.qm@web50603.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070116141359.01b3adb8@gemini.msu.montana.edu> Eileen, I suspect the water is the source of your problem. Distilled water stored in a polypropylene carboys can have saprophytic acid fast organisms growing in the water ( a published observation many years ago). If you use any distilled water from this type of storage source to make up stains, as a buffer for stains or to make up buffers, be sure to wash and Clorox the container before you refill it. I have seen huge distilled water tanks turn green with algae growth also. There may be other bacteria that grow in distilled water too, hence careful washing of waterbath flotation dish. If you add an adhesive (ready to use, liquid form) to the waterbath, that can be a problem too. We had one commercial chrome alum subbing adhesive develop some very nice growth in it. At 01:20 PM 1/16/2007, you wrote: >Hi Everyone, > > We are having a major problem with AFB, Giemsa and know Fe staining. On > the slides its self, outside the tissue, we are getting AFB, Giemsa and > Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are > stained on a staining rack. Our stainer recently had the water filter > changed. This is occurring with all the Techs. We use charged slides for > all specials, > Could this be in the paraffin? The charged slides? > All suggestions are appreciated. > > Eileen > Edward Hospital > > >--------------------------------- >Finding fabulous fares is fun. >Let Yahoo! FareChase search your favorite travel sites to find flight and >hotel bargains. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From dellav <@t> musc.edu Tue Jan 16 15:39:47 2007 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Tue Jan 16 15:42:53 2007 Subject: [Histonet] Bacteria on the slides Message-ID: Eileen, I agree with Liz that maintaining scrupulously clean water baths is a key component to avoiding the problem you are describing. One of our staff had brought in one of those green teflon type scrubbing pads like you migh use at home and we discovered this thing was growing bacteria. imagine what it's doing in your kitchen at home. also, it is quite common to find bacteria of the sort you are describing in your tap water. you mentioned using filtered water for your stainer. are you using the same filtered water for your water baths? if you aren't, tap water is commonly teeming with bugs. even with a filter change, check to see what particle size your filter removes. we added a sub-micron filter to our deionizing system to eliminate particles more than 0.5 micron and larger which eliminates the bacteria getting into the system from the tap water. one last point, to save you another possible headache. when our problem surfaced, one of our pathologists suggested we purchase sterile water for AFB stains. keep in mind sterile water is not guaranteed not to contain bacteria, they just arent' alive so don't assume sterile water will solve your problem. filtering out the bugs and meticulously cleaning the water baths are your best bets. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> eileen dusek 01/16/07 03:20PM >>> Hi Everyone, We are having a major problem with AFB, Giemsa and know Fe staining. On the slides its self, outside the tissue, we are getting AFB, Giemsa and Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are stained on a staining rack. Our stainer recently had the water filter changed. This is occurring with all the Techs. We use charged slides for all specials, Could this be in the paraffin? The charged slides? All suggestions are appreciated. Eileen Edward Hospital --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chris <@t> biocare.net Tue Jan 16 15:46:02 2007 From: chris <@t> biocare.net (Chris Von Vedder) Date: Tue Jan 16 15:46:13 2007 Subject: [Histonet] unsubscribe Message-ID: <20070116214605.TZNC14412.imta06a2.registeredsite.com@CVonVedder> From barry_m <@t> ozemail.com.au Tue Jan 16 16:11:24 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Tue Jan 16 16:11:37 2007 Subject: [Histonet] guidelines In-Reply-To: <006c01c738c6$9fdba300$6501a8c0@Patsy> References: <0E394B648E5284478A6CCB78E5AFDA2703640916@hpes1.HealthPartners.int> <006c01c738c6$9fdba300$6501a8c0@Patsy> Message-ID: <001601c739bb$43554dc0$0201010a@WORKSTATION1> Been on the fixation bandwagon for years ever since the word turn around times became fashionable with management. I certainly notice the effects of lack of adequate fixation and processing on Immunohistochemistry. Regards Barry Madigan Immunohistochemistry QHPS Central Royal Brisbane Hospital Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, 16 January 2007 3:00 AM To: 'Webb, Dorothy L'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] guidelines Dorothy et al, One reason for the new Her2 guidelines from CAP is that there has been a discrepancy in results when samples are not adequately fixed in formalin and/or alcohol fixed. If the sample is not adequately cross linked by formalin fixation before it is processed, alcohols can damage or destroy the Her2 protein making it unavailable for detection. Your best bet is to fix the sample for 24 hrs. in formalin, then it will be protected from processing and can be retrieved but at least it is still there. I know, who has time to fix for 24hrs., well an approach to that could be that you take a piece of the sample and process it quickly for the H&E, while leaving another piece to fix for 24hrs., by the time the H&E is read and Her2 ordered your sample will be properly fixed, will withstand paraffin processing, then you do the IHC on the fixed piece. Requiring a minimum of 6 hrs fixation in formalin is how CAP is starting to address this problem. I expect that I am opening up a can of worms here but I think this is what this is about. Studies have been done in labs using alcohol fixatives and/or inadequate formalin fixation that show a drop in up to 30% of positive Her2 cases. That should be telling. Patsy From Robert.Lott <@t> TriadHospitals.com Tue Jan 16 16:53:41 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Tue Jan 16 16:54:17 2007 Subject: [Histonet] Help finding Bryan Hewlett Message-ID: <673832E27C45FC4D97EF758FFC777C27019E0B04@CPRTEVS01.triadhospitals.net> Hi Everyone, I am interested in finding Bryan Hewlett.... Can anyone help me with a current e-mail address? Bryan R. Hewlett ART, MLT Technical Specialist (Retired) Anatomical Pathology, McMaster University Medical Centre Hamilton, Ontario, Canada Thanks! Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com From mtarango <@t> nvcancer.org Tue Jan 16 19:53:48 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Jan 16 19:54:16 2007 Subject: [Histonet] CD133 Help!! Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990B@NVCIEXCH02.NVCI.org> If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From TMcNemar <@t> lmhealth.org Wed Jan 17 06:53:00 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jan 17 06:55:58 2007 Subject: [Histonet] Bacteria on the slides Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F449@lmhsmail.lmhealth.org> We had a similar problem awhile back. We cut certain controls in bulk and store them in slide boxes. All of a sudden we started getting fungus on our slides. After going through the usual troubleshooting, we traced it back to the storage of the control slides. We had always cut our slides and immediately put them in the boxes while they were still wet. We got new slide boxes and started letting the slides dry completly before putting them away. The problem disappeared and hasn't been seen since. Apparantly, the dampness of the slides combined with the humidity of the sealed slide box was a perfect environment for growing fungus. (duh, imagine that!) Anyway, just a thought. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of eileen dusek Sent: Tuesday, January 16, 2007 3:21 PM To: histonet Subject: [Histonet] Bacteria on the slides Hi Everyone, We are having a major problem with AFB, Giemsa and know Fe staining. On the slides its self, outside the tissue, we are getting AFB, Giemsa and Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are stained on a staining rack. Our stainer recently had the water filter changed. This is occurring with all the Techs. We use charged slides for all specials, Could this be in the paraffin? The charged slides? All suggestions are appreciated. Eileen Edward Hospital --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Derek.Papalegis <@t> tufts.edu Wed Jan 17 08:00:56 2007 From: Derek.Papalegis <@t> tufts.edu (Derek Papalegis) Date: Wed Jan 17 08:01:04 2007 Subject: [Histonet] richard allen processor Message-ID: <20070117090056.nxjxb3oo6koo0s4s@webmail.tufts.edu> Could anyone give me some feedback about Richard Allen's STP 120 taple top spin processor? I have heard mixed things about it. some say the spin processors are junk and some say that this Richard Allen model is great. I would appreciate any feedback at all. Thanks, Derek From twheelock <@t> mclean.harvard.edu Wed Jan 17 08:25:39 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 17 08:05:25 2007 Subject: [Histonet] One more thing regarding Luxol Fast Blue differentiator Message-ID: <45AE31E3.80603@mclean.harvard.edu> Hi Everyone: In regards to the Luxol Fast Blue differentiator that I use (1% hydroquinone, 5% sodium sulfite aqueous) I forgot to mention that you can simply batch the slides through this solution (in fact through the entire protocol). (I use 60 slide racks). You do not have to differentiate them one at a time. Tim Wheelock Harvard Brain Tissue Resource Center 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From lblazek <@t> digestivespecialists.com Wed Jan 17 08:23:18 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jan 17 08:27:10 2007 Subject: [Histonet] richard allen processor Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684CB6@bruexchange.digestivespecialists.com> Derek, I used the 120 until I out grew it and found it to be a good processor. If you don't have a high volume of work it does the job. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Wednesday, January 17, 2007 9:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] richard allen processor Could anyone give me some feedback about Richard Allen's STP 120 taple top spin processor? I have heard mixed things about it. some say the spin processors are junk and some say that this Richard Allen model is great. I would appreciate any feedback at all. Thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 17 09:01:13 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 17 09:01:23 2007 Subject: [Histonet] Bacteria on the slides In-Reply-To: <406375.87375.qm@web50603.mail.yahoo.com> Message-ID: <20070117150113.97376.qmail@web61218.mail.yahoo.com> Eileen: Bacteria can grow on any stored water or buffer solutions and this includes the container itself and the tubbing used with the container. Weekly thorough cleaning of the containers, and regular replacement of the tubbing usualy prevent the bacteria to grow and contaminate your slides. Ren? J. eileen dusek wrote: Hi Everyone, We are having a major problem with AFB, Giemsa and know Fe staining. On the slides its self, outside the tissue, we are getting AFB, Giemsa and Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are stained on a staining rack. Our stainer recently had the water filter changed. This is occurring with all the Techs. We use charged slides for all specials, Could this be in the paraffin? The charged slides? All suggestions are appreciated. Eileen Edward Hospital --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From gcallis <@t> montana.edu Wed Jan 17 09:57:45 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jan 17 09:57:46 2007 Subject: [Histonet] One more thing regarding Luxol Fast Blue differentiator In-Reply-To: <45AE31E3.80603@mclean.harvard.edu> References: <45AE31E3.80603@mclean.harvard.edu> Message-ID: <6.0.0.22.1.20070117085405.01b3f8a0@gemini.msu.montana.edu> Does anyone have a reference for using this differentiator? I have always used the lithium differentiator step with great success and also batch slides. At 07:25 AM 1/17/2007, you wrote: >Hi Everyone: > >In regards to the Luxol Fast Blue differentiator that I use (1% >hydroquinone, 5% sodium sulfite aqueous) I forgot to mention that you can >simply batch the slides through this solution (in fact through the entire >protocol). (I use 60 slide racks). You do not have to differentiate them >one at a time. > >Tim Wheelock >Harvard Brain Tissue Resource Center >617-855-3592 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Nalini.Makhijani <@t> va.gov Wed Jan 17 10:07:44 2007 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Wed Jan 17 10:08:06 2007 Subject: [Histonet] CD133 Help!! In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE1F990B@NVCIEXCH02.NVCI.org> Message-ID: <715FA772CEA81A47B1A762AB6E45123A2733D6@VHAV22MSGA3.v22.med.va.gov> Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fmoraes <@t> igc.gulbenkian.pt Wed Jan 17 10:18:10 2007 From: fmoraes <@t> igc.gulbenkian.pt (Filipa Pontes de Moraes) Date: Wed Jan 17 10:18:32 2007 Subject: [Histonet] Immuno-fluorescence: maximum time in Ab solution? Message-ID: <7FAA680E-4F59-4ECB-B427-6C213B6120D3@igc.gulbenkian.pt> Hi I planning to perform a whole mount double immuno-fluorescence for =20 vessels and smooth muscle cells ( 1Ab solution: rat anti-mouse =20 Pecam) (2nd Ab sol: Goat anti-rat Alexa488 together with Cy3 =20 conjugated mouse antiSMA) using mouse embryos. I would like to know =20 what could be the maximum time of 2ndAb solution? Usually is o/n at =20 4=FBC What could happen if instead of one night the embryos stayed 2 =20 days in 2nd Ab solution? What is the maximum time the embryos could stay mounted in the slides =20= without loosing the signal? I am planning to mount them in methyl =20 salicilate (SIGMA) used as the clearing agent. I am having problems with this clearing agent Methyl salicilate, it =20 damage my embryos i just add drop by drop and it is still very =20 difficult to avoid damaging the E10.5 embryos. Can you advice me =20 other organic clearing agent? i have seen 1 benzil alcohol : 2 =20 benzil benzoate used as cleaing agent and mounting reagent also is it =20= better? Thank You very much Filipa Filipa From rjbuesa <@t> yahoo.com Wed Jan 17 10:23:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 17 10:23:39 2007 Subject: [Histonet] CD133 Help!! In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE1F990B@NVCIEXCH02.NVCI.org> Message-ID: <269181.5019.qm@web61222.mail.yahoo.com> Mark: I have not worked with this Ab but as a rule, when I did not get a clear reaction, I used to work with the HIER pH or the dilution, rather than with the incubation time. No matter how long you increase the reaction time, if there is not enough Ab concentration (or epitope signal for that matter) you will not get an intense result. Ren? J. "Tarango, Mark" wrote: If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From sheila_adey <@t> hotmail.com Wed Jan 17 10:32:10 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Jan 17 10:32:23 2007 Subject: [Histonet] frozen section fixative In-Reply-To: <6.0.0.22.1.20070116112135.01b83708@gemini.msu.montana.edu> Message-ID: We use 10% NBF with 50% alcohol. Half of each Sheila Adey HT MLT Port Huron Hospital Michigan >From: Gayle Callis >To: "Tapper, Sheila" , >Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] frozen section fixative >Date: Tue, 16 Jan 2007 11:22:15 -0700 > >neutral buffered formalin for best morphology, but have use 95% ethanol >too. > >At 10:55 AM 1/16/2007, you wrote: >>What are people using to fix their frozen sections prior to staining >>with H&E? >> >> >> >>Thanks! >> >> >> >>Sheila Tapper HT(ASCP) >> >>St. Luke's Hospital >> >>Duluth, MN 55805 >> >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your opinion matters. Please tell us what you think and be entered into a draw for a grand prize of $500 or one of 20 $50 cash prizes. http://www.youthographyinsiders.com/R.aspx?a=116 From Melissa.Zummak <@t> ssfhs.org Wed Jan 17 10:33:53 2007 From: Melissa.Zummak <@t> ssfhs.org (Zummak Melissa) Date: Wed Jan 17 10:34:01 2007 Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility Message-ID: Hi everyone, Does anyone know where I can find recommendations for checking\monitoring temperature reproducibility for microwaves, I only use the microwave for certain special stains currently. Melissa Zummak Alverno Clinincal Lab From pruegg <@t> ihctech.net Wed Jan 17 10:35:37 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jan 17 10:35:49 2007 Subject: [Histonet] CD133 Help!! In-Reply-To: <715FA772CEA81A47B1A762AB6E45123A2733D6@VHAV22MSGA3.v22.med.va.gov> Message-ID: <002001c73a55$8524a170$6501a8c0@Patsy> Which cd133 are you using? If you are using the antibody from Miltyeni I suggest you toss it and get the one from Abcam rab polyclonal ab19898, I use this at 1:100 overnight after HIER steam in HpH buffer for 25 min. with rab labeled polymer hrp detection for 45 min. and dab for 15 min. This antibody as well as many others has been discussed on the NSH IHC Resource Group list serve, to join this group go to www.ihcrg.org and submit a membership form online. We have a list serve similar to histonet that focuses just on IHC/Molecular Diagnostics isssues. Patsy Ruegg Chair, IHCRG -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Makhijani, Nalini S Sent: Wednesday, January 17, 2007 9:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Jan 17 10:39:45 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jan 17 10:40:06 2007 Subject: [Histonet] Bacteria on the slides In-Reply-To: <6.0.0.22.1.20070116141359.01b3adb8@gemini.msu.montana.edu> Message-ID: <002101c73a56$18c91be0$6501a8c0@Patsy> Deionized water can also be contaminated with organisms and certainly the tap water. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, January 16, 2007 2:26 PM To: eileen dusek; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bacteria on the slides Eileen, I suspect the water is the source of your problem. Distilled water stored in a polypropylene carboys can have saprophytic acid fast organisms growing in the water ( a published observation many years ago). If you use any distilled water from this type of storage source to make up stains, as a buffer for stains or to make up buffers, be sure to wash and Clorox the container before you refill it. I have seen huge distilled water tanks turn green with algae growth also. There may be other bacteria that grow in distilled water too, hence careful washing of waterbath flotation dish. If you add an adhesive (ready to use, liquid form) to the waterbath, that can be a problem too. We had one commercial chrome alum subbing adhesive develop some very nice growth in it. At 01:20 PM 1/16/2007, you wrote: >Hi Everyone, > > We are having a major problem with AFB, Giemsa and know Fe staining. On > the slides its self, outside the tissue, we are getting AFB, Giemsa and > Fe staining. The AFB is brought down to water by hand, AFB and Giemsa are > stained on a staining rack. Our stainer recently had the water filter > changed. This is occurring with all the Techs. We use charged slides for > all specials, > Could this be in the paraffin? The charged slides? > All suggestions are appreciated. > > Eileen > Edward Hospital > > >--------------------------------- >Finding fabulous fares is fun. >Let Yahoo! FareChase search your favorite travel sites to find flight and >hotel bargains. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Jan 17 10:53:15 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jan 17 10:53:29 2007 Subject: [Histonet] VEGFR1 and 2 in rat In-Reply-To: <008001c73998$35dd3450$ae0b8286@ZION> Message-ID: <002301c73a57$fbc75960$6501a8c0@Patsy> Peter, I use Flt-1/Vegfr1 and Flk-1/KDR/Vegfr2 from Lab Vision now ThermoFisher cat #RB1527 andRB9239 respectively. For both, HIER 20' with citrate buffer ab dilution 1:25 - 1:50 for 60' with rab labeled polymer hrp for 30' and dab 10' on rat tissue. Angiosarcoma is a good control for these. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boor Peter Sent: Tuesday, January 16, 2007 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VEGFR1 and 2 in rat Hi! Can anyone recommend an antibody for VEGFR 1 and 2 that works well in rat? We only tested anti-human polyclonals (R&D) with various Ag retrieval protocols with no result. Also, there are Ab against human phosphorylated VEGFR that should be apliable for IH (Calbiochem), do they work also in rat tissue? Thanks a lot for any information! Peter Boor Peter Boor University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. 30; 52074 Aachen; Germany Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany Mob.: 0049 (0) 177 2345 163 Lab: 0049 (0) 241 80 89670 Fax: 0049 (0) 241 80 82446 ICQ Nr.: 259 368 084 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Wed Jan 17 11:15:55 2007 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Wed Jan 17 11:18:02 2007 Subject: [Histonet] RE:Information on Antibodies Message-ID: <20070117.091609.2157.507172@webmail45.nyc.untd.com> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) From rjbuesa <@t> yahoo.com Wed Jan 17 11:28:50 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 17 11:28:57 2007 Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility In-Reply-To: Message-ID: <283520.90961.qm@web61225.mail.yahoo.com> Melissa: You should calibrate your MW oven, meaning, find out the real wattage, and prepare heating curves for different volumes of liquid. Once you have that, prepare a log that will reflect the temperature reached by a given volume of liquid (preferable distilled water) after a selected heating period. The temperature should be the same everytime you perform this task. This will document the reproducibility of your MW oven. If you want I can send you the calibration procedure (although this subject can be found also in Histonet archieves; it has been a frequent topis of discussion). Ren? J. Zummak Melissa wrote: Hi everyone, Does anyone know where I can find recommendations for checking\monitoring temperature reproducibility for microwaves, I only use the microwave for certain special stains currently. Melissa Zummak Alverno Clinincal Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From RBARNHART <@t> summithealth.org Wed Jan 17 11:45:51 2007 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Wed Jan 17 11:47:01 2007 Subject: [Histonet] Bacteria on the slides Message-ID: We had this problem several months ago and determined the bacteria was coming from the water rinse in the stainer. The buckets had a slimmy film we think because the water coming into the stainer does not have a high flow. So now we pull the buckets and bleach every month or so. From failm <@t> musc.edu Wed Jan 17 12:01:10 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Wed Jan 17 12:02:02 2007 Subject: [Histonet] RE:Information on Antibodies Message-ID: Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica <@t> medstaffservices.com Wed Jan 17 12:20:35 2007 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Jan 17 12:20:44 2007 Subject: [Histonet] EMPLOYMENT OPPROTUNITIES Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: ~$30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k 3. Generalist Hours: Monday - Friday, Day Shift Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From twheelock <@t> mclean.harvard.edu Wed Jan 17 12:50:20 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 17 12:30:11 2007 Subject: [Histonet] Luxol Reference Message-ID: <45AE6FEC.3000703@mclean.harvard.edu> Hi Gayle: As I was mentioning to Andi, I myself cannot find a reference for the protocol. I was taught the stain at the Massachusetts General Hospital back in 1984. (It was simply typed on a 3 x 5 file card) I took a copy of the recipe with me when I left the General in 1986. The neuropathology lab was liquidated some years ago, and it's functions integrated into general pathology. Perhaps, someone in Surgical Pathology or Special Stains at MGH could tract it down. The main number at MGH is 617-726-2000 It is very interesting though that the exact same "differentiator" of the Luxol (1% hydroquinone, 5% sodium sulfite, aqueous) is used for the reducing agent in the Bodian protein-silver stain. We used it for both purposes at the MGH., although I use the Bielschowsky silver stain instead here at McLean One can find find a reference for the Bodian, at least, in Sheehan's "Theory and Practice of Histotechnology", second edition,, page 256. By the way, In my first email I did not mean to disparage the use of Lithium Carbonate. Every version of the stain I have seen in the Histology books uses Lithium. So it must work perfectly well in most labs, such as your own. I see my protocol as just an alternative to Lithium just as the Lithium would definitely be an alternative for me if I had problems with the Hydroquinone. Thank you for the detailed description of the Lithium Carbonate technique; I will keep it on hand. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From mtarango <@t> nvcancer.org Wed Jan 17 12:45:21 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Jan 17 12:45:48 2007 Subject: [Histonet] CD133 Help!! Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990D@NVCIEXCH02.NVCI.org> I tried that, but no luck. Did you have some success incubating the primary overnight? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Makhijani, Nalini S [mailto:Nalini.Makhijani@va.gov] Sent: Wednesday, January 17, 2007 8:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From mtarango <@t> nvcancer.org Wed Jan 17 12:47:38 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Jan 17 12:49:14 2007 Subject: [Histonet] CD133 Help!! Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990E@NVCIEXCH02.NVCI.org> I have 3 different CD133/AC133 antibodies. I can't get even one of them to work. I'll have to buy the one you suggest. Thanks Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Wednesday, January 17, 2007 8:36 AM To: 'Makhijani, Nalini S'; Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Which cd133 are you using? If you are using the antibody from Miltyeni I suggest you toss it and get the one from Abcam rab polyclonal ab19898, I use this at 1:100 overnight after HIER steam in HpH buffer for 25 min. with rab labeled polymer hrp detection for 45 min. and dab for 15 min. This antibody as well as many others has been discussed on the NSH IHC Resource Group list serve, to join this group go to www.ihcrg.org and submit a membership form online. We have a list serve similar to histonet that focuses just on IHC/Molecular Diagnostics isssues. Patsy Ruegg Chair, IHCRG -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Makhijani, Nalini S Sent: Wednesday, January 17, 2007 9:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Nalini.Makhijani <@t> va.gov Wed Jan 17 13:48:46 2007 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Wed Jan 17 13:48:55 2007 Subject: [Histonet] CD133 Help!! In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE1F990D@NVCIEXCH02.NVCI.org> Message-ID: <715FA772CEA81A47B1A762AB6E45123A2733D7@VHAV22MSGA3.v22.med.va.gov> I've never used this antibody- I was just suggesting a general technique that I have seen work with overnight incubation rather than with a 1 hour incubation, e.g.with Pecam 1. -----Original Message----- From: Tarango, Mark [mailto:mtarango@nvcancer.org] Sent: Wednesday, January 17, 2007 10:45 AM To: Makhijani, Nalini S; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! I tried that, but no luck. Did you have some success incubating the primary overnight? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Makhijani, Nalini S [mailto:Nalini.Makhijani@va.gov] Sent: Wednesday, January 17, 2007 8:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== From twheelock <@t> mclean.harvard.edu Wed Jan 17 15:02:20 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 17 14:42:03 2007 Subject: [Histonet] LINES IN PARAFFIN SECTIONS Message-ID: <45AE8EDC.7020903@mclean.harvard.edu> Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From rjbuesa <@t> yahoo.com Wed Jan 17 14:52:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 17 14:52:39 2007 Subject: [Histonet] LINES IN PARAFFIN SECTIONS In-Reply-To: <45AE8EDC.7020903@mclean.harvard.edu> Message-ID: <20070117205230.53771.qmail@web61225.mail.yahoo.com> Tim: I would try first changing to another brand of disposable (JUST to see if there is a difference). If the problem persists I would return to your usual disposables and then try adding some bees wax (5%) to your paraffin (JUST to see if there is a difference). If the problem remains it could be some dust in the paraffin or in the molds; have you had problems with the air filters in your lab? Just some ideas to try to isolate the problem. I hope this would help you! Ren? J. Tim Wheelock wrote: Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. From JWEEMS <@t> sjha.org Wed Jan 17 14:59:01 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jan 17 14:59:41 2007 Subject: [Histonet] LINES IN PARAFFIN SECTIONS Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3046@sjhaexc02.sjha.org> And you might run a piece of cork (like from a wine bottle) across your blade before you change them - you may have a lot with rough edges... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, January 17, 2007 3:53 PM To: Tim Wheelock; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] LINES IN PARAFFIN SECTIONS Tim: I would try first changing to another brand of disposable (JUST to see if there is a difference). If the problem persists I would return to your usual disposables and then try adding some bees wax (5%) to your paraffin (JUST to see if there is a difference). If the problem remains it could be some dust in the paraffin or in the molds; have you had problems with the air filters in your lab? Just some ideas to try to isolate the problem. I hope this would help you! Ren? J. Tim Wheelock wrote: Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 17 15:14:46 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Jan 17 15:14:53 2007 Subject: [Histonet] LINES IN PARAFFIN SECTIONS Message-ID: Tim Sounds to me that you have a cutting problem or problems, with thickness variation and lines. I am assuming that your tissue is relatively uniform in consistency? I suggest problem might be one of the following. 1. Is the knife securely clamped into the holder? If there is any movement at all on one side this might explain the thickness differences. 2. Does the holder have wear on one side, either the knife holder or the sliding mechanism it sits on? 3. Are these blades reliable as regards having a sharp edge along entire edge? Rene's suggestion should take care of this. 4. I do not believe that the wax is the problem unless you have poorly mixed wax or separation of the components. If this is the problem then this effect should be seen on different sides of different blocks in a random manner. If this wax is too hard then Rene's suggestion of beeswax should help Would you be kind enough to let us know what size blocks you are cutting? thanks Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 17, 2007 2:53 PM To: Tim Wheelock; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] LINES IN PARAFFIN SECTIONS Tim: I would try first changing to another brand of disposable (JUST to see if there is a difference). If the problem persists I would return to your usual disposables and then try adding some bees wax (5%) to your paraffin (JUST to see if there is a difference). If the problem remains it could be some dust in the paraffin or in the molds; have you had problems with the air filters in your lab? Just some ideas to try to isolate the problem. I hope this would help you! Ren? J. Tim Wheelock wrote: Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Jan 17 15:15:32 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jan 17 15:16:24 2007 Subject: [Histonet] Racemace/p63 cocktail Message-ID: Does any one know of a vendor that has a cocktail for p63 and alpha-Methylacyl-CoA Racemace (AMACR). I need it for FFPE human tissue. I believe that people refer to the latter in many different ways- Racemace; AMACR; P506S Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From pruegg <@t> ihctech.net Wed Jan 17 15:16:11 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jan 17 15:16:29 2007 Subject: [Histonet] looking for Andra Erickson Message-ID: <00c201c73a7c$b75c8c80$6501a8c0@Patsy> Andra, Please email me with your correct email address, the application for the IHC Resource Group did not have a correct email address. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From gcallis <@t> montana.edu Wed Jan 17 15:14:05 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jan 17 15:16:37 2007 Subject: Fwd: Re: [Histonet] LINES IN PARAFFIN SECTIONS Message-ID: <6.0.0.22.1.20070117141120.01b10178@gemini.msu.montana.edu> >Stir the paraffin just before embedding to redistribute the plastic >polymers and other additives in paraffin. These tend to settle out at >melting and being heavier, will settle to bottom of the dispenser. Little >blobs of stuff often appear, but more likely end up in your embedding >molds, creating problems. A cheap spatula does the trick. We just encountered this problem with another brand of paraffin - stirring cured the problem. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From twheelock <@t> mclean.harvard.edu Wed Jan 17 15:57:12 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 17 15:37:03 2007 Subject: [Histonet] LINES IN PARAFFIN SECTIONS 2 Message-ID: <45AE9BB8.4080302@mclean.harvard.edu> Hi Barry: I am cutting blocks that can go up to twice as large as a postage stamp, although some are half that size or less. The brain tissue is somewhat heterogeneous in density because of the variable distribution of gray and matter in the same block. I am also going to try blank blocks, first thing in the morning, to see if there is something in the paraffin. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From DGNajarian <@t> comcast.net Wed Jan 17 15:42:43 2007 From: DGNajarian <@t> comcast.net (Dennis Najarian) Date: Wed Jan 17 15:43:03 2007 Subject: [Histonet] One more thing regarding Luxol Fast Blue differentiator In-Reply-To: <45AE31E3.80603@mclean.harvard.edu> Message-ID: Please unsubscribe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, January 17, 2007 9:26 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing regarding Luxol Fast Blue differentiator Hi Everyone: In regards to the Luxol Fast Blue differentiator that I use (1% hydroquinone, 5% sodium sulfite aqueous) I forgot to mention that you can simply batch the slides through this solution (in fact through the entire protocol). (I use 60 slide racks). You do not have to differentiate them one at a time. Tim Wheelock Harvard Brain Tissue Resource Center 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Jan 17 16:25:46 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jan 17 16:26:01 2007 Subject: FW: Re: [Histonet] Zeiss Microm Cryostat (D6900) Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30EBA@PHSXMB1.partners.org> To All: I am trying to find out who services this cryostat. Zeiss stopped servicing this a while back and a transitional company took over. I do not remember who that was or if they still are the ones to contact--they did not offer service contracts. There is "nothing" wrong with the cryostat, but it could use an overhaul (P.M.). Thank you! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. From Jan.Minshew <@t> leica-microsystems.com Wed Jan 17 18:35:28 2007 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Wed Jan 17 18:35:47 2007 Subject: [Histonet] LINES IN PARAFFIN SECTIONS In-Reply-To: <45AE8EDC.7020903@mclean.harvard.edu> Message-ID: Hi Tim, You have gotten some excellent suggestions so far, but I thought I might ask a few more questions to see if the problem can be isolated a little better. Like Barry, I don't think the problem is caused entirely by the disposable blades or the paraffin. Unfortunately, there are a LOT of other things to consider. Do you turn your paraffin reservoir on the embedding station off and on daily? If so, it can cause problems if the paraffin does not have time to melt completely because different additives have different melting points and come out of the solid block at different times. If you want to save energy and turn it off when you are not using it, use as little paraffin as possible so it will melt completely in the allotted time. As Gayle suggested, you should always stir the paraffin right before you use it to evenly distribute all of the constituents. Do you embed using cassettes? I know this sounds odd, but if your cassettes are not filled up high enough with paraffin the front portion of the block (the part with the specimen) can become loose and have just enough movement to cause problems. If you are making blocks (not using cassettes) are you clamping down directly on the paraffin. Tightening down directly on the paraffin can sometimes cause sectioning variances, especially if you tighten really hard. It's better to mount the block on a wooden, plastic or metal plate and clamp down on that. Are you fixing in 10% neutral buffered formalin? If so, are you going from fixative into an alcohol that is 70% or less in strength. Higher concentrations can cause the buffer salts to precipitate in your solutions and the tissue. This can make your blocks look like shredded wheat when you section them. Which microtome do you use...how old is it...and how well is it maintained? Have you thoroughly inspected the front and back pressure plates on the blade holder? If there are any irregularities or uneven wear, it will cause uneven pressure on the blade and produce uneven sectioning like you described. Does the pressure plate have an adjustment screw (or two) that could be improperly aligned? How much pressure is being exerted on the blade? People think that if a little is good then a lot is better, but that's not correct in this instance. The pressure plate should rest evenly across the blade (the blade should be centered under the plate if possible) and only a slight amount of pressure should be applied--just enough to GENTLY hold the blade in place and not enough to cause the blade to bow. Too much pressure will also cause the problems you described. Are all of the locking mechanisms on the microtome, knife holder base, disposable blade holder, x-y orientation device and specimen holder locking correctly? Do the locking levers feel like they are locking properly--not rotating past the locking point if you push them and not stopping in their rotation before you feel they should? Can you physically move any of the pieces when they are locked down? Please feel free to contact me or one of Leica's excellent Technical Applications Center team members at 800.248.0123 if you are unable to resolve your problem or have any further questions. There are a lot of things that can go wrong and cause sectioning problems and we would be happy to help in any way we can. Best wishes, Jan Minshew, HT(ASCP)HTL Marketing Manager Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 x7051 toll free 847.405.7051 direct 847.405.6560 fax jan.minshew@leica-microsystems.com Tim Wheelock To Sent by: Histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] LINES IN PARAFFIN SECTIONS 2 01/17/2007 03:57 PM Hi Barry: I am cutting blocks that can go up to twice as large as a postage stamp, although some are half that size or less. The brain tissue is somewhat heterogeneous in density because of the variable distribution of gray and matter in the same block. I am also going to try blank blocks, first thing in the morning, to see if there is something in the paraffin. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Tim Wheelock To Sent by: Histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] LINES IN PARAFFIN SECTIONS 01/17/2007 03:02 PM Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From rchiovetti <@t> yahoo.com Wed Jan 17 21:03:04 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Wed Jan 17 21:03:16 2007 Subject: [Histonet] Zeiss Microm Cryostat (D6900) Message-ID: <20070118030305.11663.qmail@web58901.mail.re1.yahoo.com> Peggy, It all depends on how the territory is covered in your area. Microm is now part of Richard-Allan Scientific, which is now part of Thermo... There is undoubtedly a regional rep for Richard-Allan or Thermo somewhere near you, or perhaps a third-party (independent) group who could service the cryostat. You'll probably get more info from others who respond. A starting point is Richard-Allan's website. The page which goes directly to service and contact info is: Hope this helps! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner Southwest Precision Instruments The Desert Southwest's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: "Sherwood, Margaret " To: histonet@lists.utsouthwestern.edu Sent: Wednesday, January 17, 2007 3:25:46 PM Subject: FW: Re: [Histonet] Zeiss Microm Cryostat (D6900) To All: I am trying to find out who services this cryostat. Zeiss stopped servicing this a while back and a transitional company took over. I do not remember who that was or if they still are the ones to contact--they did not offer service contracts. There is "nothing" wrong with the cryostat, but it could use an overhaul (P.M.). Thank you! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news From musselmannk <@t> mail.nih.gov Thu Jan 18 08:06:06 2007 From: musselmannk <@t> mail.nih.gov (Kurt Musselmann) Date: Thu Jan 18 08:07:16 2007 Subject: [Histonet] Help with a Reichart Jung Frigocut 2800 Message-ID: Hello everybody I am new to the list, and was directed to you guys by Alan Bright. I have started using a Reichart Jung Frigocut 2800, and we have a badly chipped anti-roll plate. Our plate measures roughly 5x3x0.3 cm, and I am trying to find a replacement for it. Does anybody know of a company that manufactures anti-roll plates of that size, or would I have to change the whole anti-roll device (I had ordered one from Electron Microscope Sciences (EMS) but it did not fit our machine and had to be returned. I appreciate any input Kurt Musselmann -- Kurt Musselmann Ph.D. NIDCR/NIH 30 Convent Drive Building 30, Room 403 Bethesda, MD 20892-4370 (301) 496 3065 From Melissa.Zummak <@t> ssfhs.org Thu Jan 18 08:09:44 2007 From: Melissa.Zummak <@t> ssfhs.org (Zummak Melissa) Date: Thu Jan 18 08:09:54 2007 Subject: [Histonet] Zeiss Microm Cryostat (D6900) Message-ID: Peggy, I will give you the number of the person who sets up my service contracts for my instruments, he might be able to help you either with the company I use or find someone located close to you. His name is Rob Mills and business number is 866-497-3033. Melissa Zummak AP Supervisor Alverno Clinical labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, January 17, 2007 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Zeiss Microm Cryostat (D6900) To All: I am trying to find out who services this cryostat. Zeiss stopped servicing this a while back and a transitional company took over. I do not remember who that was or if they still are the ones to contact--they did not offer service contracts. There is "nothing" wrong with the cryostat, but it could use an overhaul (P.M.). Thank you! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Thu Jan 18 08:21:32 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Thu Jan 18 08:22:48 2007 Subject: [Histonet] RE:Information on Antibodies Message-ID: <45AF11EC020000D6000306CE@mail.mrl.ubc.ca> Marsha I have used D2-40. What sort of info are you looking for? MArk >>> "Mildred Fail" 1/17/2007 10:01:10 AM >>> Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From settembr <@t> umdnj.edu Thu Jan 18 08:36:28 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu Jan 18 08:41:30 2007 Subject: [Histonet] CK 19 Message-ID: Hello Marsha, I use CK 19 on FFPE human tissue. I use Dako's cat.# M0888 It is an In Vitro Diagnostic antibody. I use it at a dilution of 1:25 and incubate 15 mnutes RT I also use their Target Retreival Solution and use a papillary carcinoma as a positive control. I get nice results. Good Luck Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Mark Elliott 01/18/07 9:21 AM >>> Marsha I have used D2-40. What sort of info are you looking for? MArk >>> "Mildred Fail" 1/17/2007 10:01:10 AM >>> Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Don.Birgerson <@t> leica-microsystems.com Thu Jan 18 08:44:44 2007 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Thu Jan 18 08:45:03 2007 Subject: [Histonet] Help with a Reichert Jung Frigocut 2800 In-Reply-To: Message-ID: Hello Kurt, I believe we still have anti roll plates for most of our cryostats. I will call you on the phone so we can play a game of twenty questions to identify your holder. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 (7:00 to 4:00 CT) Kurt Musselmann To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Help with a Reichart Jung Frigocut 2800 01/18/2007 08:06 AM Hello everybody I am new to the list, and was directed to you guys by Alan Bright. I have started using a Reichart Jung Frigocut 2800, and we have a badly chipped anti-roll plate. Our plate measures roughly 5x3x0.3 cm, and I am trying to find a replacement for it. Does anybody know of a company that manufactures anti-roll plates of that size, or would I have to change the whole anti-roll device (I had ordered one from Electron Microscope Sciences (EMS) but it did not fit our machine and had to be returned. I appreciate any input Kurt Musselmann -- Kurt Musselmann Ph.D. NIDCR/NIH 30 Convent Drive Building 30, Room 403 Bethesda, MD 20892-4370 (301) 496 3065 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From hymclab <@t> hyhc.com Thu Jan 18 08:51:49 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Thu Jan 18 08:46:44 2007 Subject: [Histonet] Microwave procedure for monitoring temperature rep roducibility Message-ID: There is a CLSI (formerly NCCLS) Standard and Guideline book available: Microwave Device Use in the Histology Laboratory; Approved Guidelines. (description: This document provides recommendations for reproducing the performance of microwave-accelereated procedures to prepare biological specimens in the histology laboratory). Go to the CLSI website for info: www.clsi.org. I found this very helpful in fulfilling the new CAP requirements. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Zummak Melissa [mailto:Melissa.Zummak@ssfhs.org] Sent: Wednesday, January 17, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility Hi everyone, Does anyone know where I can find recommendations for checking\monitoring temperature reproducibility for microwaves, I only use the microwave for certain special stains currently. Melissa Zummak Alverno Clinincal Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From pmcardle <@t> ebsciences.com Thu Jan 18 09:24:00 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Thu Jan 18 09:24:13 2007 Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility In-Reply-To: References: Message-ID: <45AF9110.8080104@ebsciences.com> Hello everyone: And now a vendor weighs in: We have several calibration and "accreditation-related" articles available on our website (www.ebsciences.com , http://www.ebsciences.com/papers/microwave_quality.htm , http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ). In addition, we're happy to see that microwave use in the histology laboratory is finally being given the scrutiny it deserves. Many of the suggestions in CLSI's GP-28 guidelines (referenced in other postings) are excellent, for example, troubleshooting, container placement, power output determination, and overall, standardization of procedures and elimination of variables. However, I feel there are some significant problems with this publication; I have previously informed CLSI of my primary concern: Page 11, section 4.7: "A qualified authority CERTIFIED IN USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine microwave leakage..." The problem is, to our knowledge, NO SUCH CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has not been forthcoming with suggestions. This is not to imply the absence of extant legislation, Federal and otherwise, regulating the manufacture and use of microwave devices; manufacturers have to comply with FDA and other regulations regarding microwaves intended for in-vitro diagnostic use. But as for leakage detection in the field, we have yet to come across any relevant (or irrelevant, for that matter) Federal or State certification. As you can imagine, for institutions deciding to adopt the more stringent CLSI guidelines, this poses something of a "Catch-22." In addition, page 17 outlines the use of a neon bulb array, for those who aspire to eliminate "hot spots" in the microwave chamber. However, while this can be a "fun" way to indirectly observe microwave energy, unless the microwave were horribly defective, it is a fairly useless exercise. Any object (including the array itself) placed in the microwave chamber affects the distribution of microwaves, a classic example of attempted observation affecting the phenomenon observed. I want to stress, however, that overall, publication GP-28A is an outstanding effort to provide "recommendations for quality assurance and safety procedures for microwave equipment use" in their words, and to help troubleshoot and eliminate variability in results of microwave usage. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you. Zummak Melissa wrote: > Hi everyone, > > Does anyone know where I can find recommendations for > checking\monitoring temperature reproducibility for microwaves, I only > use the microwave for certain special stains currently. > > Melissa Zummak > Alverno Clinincal Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From Anna.Inman <@t> stmarygj.org Thu Jan 18 09:50:47 2007 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Thu Jan 18 09:51:06 2007 Subject: [Histonet] Microwave procedure for monitoring temperature rep roducibility Message-ID: <2925AE271EAAD440AF48FCCEB8002D090372F4C3@smgmail01.smgj.sclhs.net> What I recall about that publication......it doesn't really address microwave usage for anything other than a specimen processing angle; whereas, labs like mine only use it for heating agar/histogel to make cell blocks and things........ Anna Inman, HT(ASCP) Clinical Resource Specialist SMH Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, January 18, 2007 7:52 AM To: 'Zummak Melissa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave procedure for monitoring temperature rep roducibility There is a CLSI (formerly NCCLS) Standard and Guideline book available: Microwave Device Use in the Histology Laboratory; Approved Guidelines. (description: This document provides recommendations for reproducing the performance of microwave-accelereated procedures to prepare biological specimens in the histology laboratory). Go to the CLSI website for info: www.clsi.org. I found this very helpful in fulfilling the new CAP requirements. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Zummak Melissa [mailto:Melissa.Zummak@ssfhs.org] Sent: Wednesday, January 17, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility Hi everyone, Does anyone know where I can find recommendations for checking\monitoring temperature reproducibility for microwaves, I only use the microwave for certain special stains currently. Melissa Zummak Alverno Clinincal Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tim.morken <@t> thermofisher.com Thu Jan 18 10:33:53 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Jan 18 10:37:34 2007 Subject: [Histonet] Microwave procedure for monitoringtemperature reproducibility In-Reply-To: <45AF9110.8080104@ebsciences.com> Message-ID: Phil wrote: "Page 11, section 4.7: "A qualified authority CERTIFIED IN USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine microwave leakage..." The problem is, to our knowledge, NO SUCH CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has not been forthcoming with suggestions." That's funny, but brings up an interesting question: Who does oversight on a group like CLSI? If they went to the trouble of researching this guideline, how is it that they never (apparently) realized there is no such certification. What made them write such a requirement without knowing if such a certification exisits? Did they just assume it existed? And, if they did any lab work with an actual microwave, how could they have done so without first having it checked by a person certified to do so? The final question. If CLSI can't figure that out, should we believe them on anything else? This is a private group that has set itself up as the final word on laboratory standardization. They charge massive amounts of money for rather average informational documents (most of which, these days, anyone can find on the internet for free with a little persistance) and then something like this makes them look like fools. It seems the only possible oversight is to refuse to buy defective documents. Actually, it kinda reminds me of another body that dreams up fantasy questions to torture lab managers with... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: Thursday, January 18, 2007 7:24 AM To: Zummak Melissa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave procedure for monitoringtemperature reproducibility Hello everyone: And now a vendor weighs in: We have several calibration and "accreditation-related" articles available on our website (www.ebsciences.com , http://www.ebsciences.com/papers/microwave_quality.htm , http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ). In addition, we're happy to see that microwave use in the histology laboratory is finally being given the scrutiny it deserves. Many of the suggestions in CLSI's GP-28 guidelines (referenced in other postings) are excellent, for example, troubleshooting, container placement, power output determination, and overall, standardization of procedures and elimination of variables. However, I feel there are some significant problems with this publication; I have previously informed CLSI of my primary concern: Page 11, section 4.7: "A qualified authority CERTIFIED IN USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine microwave leakage..." The problem is, to our knowledge, NO SUCH CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has not been forthcoming with suggestions. This is not to imply the absence of extant legislation, Federal and otherwise, regulating the manufacture and use of microwave devices; manufacturers have to comply with FDA and other regulations regarding microwaves intended for in-vitro diagnostic use. But as for leakage detection in the field, we have yet to come across any relevant (or irrelevant, for that matter) Federal or State certification. As you can imagine, for institutions deciding to adopt the more stringent CLSI guidelines, this poses something of a "Catch-22." In addition, page 17 outlines the use of a neon bulb array, for those who aspire to eliminate "hot spots" in the microwave chamber. However, while this can be a "fun" way to indirectly observe microwave energy, unless the microwave were horribly defective, it is a fairly useless exercise. Any object (including the array itself) placed in the microwave chamber affects the distribution of microwaves, a classic example of attempted observation affecting the phenomenon observed. I want to stress, however, that overall, publication GP-28A is an outstanding effort to provide "recommendations for quality assurance and safety procedures for microwave equipment use" in their words, and to help troubleshoot and eliminate variability in results of microwave usage. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you. Zummak Melissa wrote: > Hi everyone, > > Does anyone know where I can find recommendations for > checking\monitoring temperature reproducibility for microwaves, I only > use the microwave for certain special stains currently. > > Melissa Zummak > Alverno Clinincal Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schraz13 <@t> yahoo.com Thu Jan 18 08:55:58 2007 From: schraz13 <@t> yahoo.com (Scheherazade Humphrey) Date: Thu Jan 18 10:42:52 2007 Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 26 Message-ID: <363901.75989.qm@web81007.mail.mud.yahoo.com> Can anyone help me find a IHC stain the is performed on the disease Blue Nevus. I am having a hard time finding out if Fontana Masson is the correct one to use and also a possible case study to reference this disease to. I had to reasearch this disease for a school project. If anyone could help me, your participation would be greatly appreciated. Thank You. histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE:Information on Antibodies (Mildred Fail) 2. EMPLOYMENT OPPROTUNITIES (Jessica Hirsch) 3. Luxol Reference (Tim Wheelock) 4. RE: CD133 Help!! (Tarango, Mark) 5. RE: CD133 Help!! (Tarango, Mark) 6. RE: CD133 Help!! (Makhijani, Nalini S) 7. LINES IN PARAFFIN SECTIONS (Tim Wheelock) 8. Re: LINES IN PARAFFIN SECTIONS (Rene J Buesa) 9. RE: LINES IN PARAFFIN SECTIONS (Weems, Joyce) 10. RE: LINES IN PARAFFIN SECTIONS (Rittman, Barry R) 11. Racemace/p63 cocktail (Dana Settembre) 12. looking for Andra Erickson (Patsy Ruegg) 13. Fwd: Re: [Histonet] LINES IN PARAFFIN SECTIONS (Gayle Callis) 14. LINES IN PARAFFIN SECTIONS 2 (Tim Wheelock) 15. RE: One more thing regarding Luxol Fast Blue differentiator (Dennis Najarian) 16. FW: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Sherwood, Margaret ) 17. Re: LINES IN PARAFFIN SECTIONS (Jan.Minshew@leica-microsystems.com) 18. Re: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Robert Chiovetti) 19. Help with a Reichart Jung Frigocut 2800 (Kurt Musselmann) 20. RE: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Zummak Melissa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 17 Jan 2007 13:01:10 -0500 From: "Mildred Fail" Subject: [Histonet] RE:Information on Antibodies To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 17 Jan 2007 13:20:35 -0500 From: "Jessica Hirsch" Subject: [Histonet] EMPLOYMENT OPPROTUNITIES To: Message-ID: Content-Type: text/plain; charset="us-ascii" CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: ~$30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k 3. Generalist Hours: Monday - Friday, Day Shift Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com ------------------------------ Message: 3 Date: Wed, 17 Jan 2007 13:50:20 -0500 From: Tim Wheelock Subject: [Histonet] Luxol Reference To: Histonet@lists.utsouthwestern.edu Message-ID: <45AE6FEC.3000703@mclean.harvard.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Gayle: As I was mentioning to Andi, I myself cannot find a reference for the protocol. I was taught the stain at the Massachusetts General Hospital back in 1984. (It was simply typed on a 3 x 5 file card) I took a copy of the recipe with me when I left the General in 1986. The neuropathology lab was liquidated some years ago, and it's functions integrated into general pathology. Perhaps, someone in Surgical Pathology or Special Stains at MGH could tract it down. The main number at MGH is 617-726-2000 It is very interesting though that the exact same "differentiator" of the Luxol (1% hydroquinone, 5% sodium sulfite, aqueous) is used for the reducing agent in the Bodian protein-silver stain. We used it for both purposes at the MGH., although I use the Bielschowsky silver stain instead here at McLean One can find find a reference for the Bodian, at least, in Sheehan's "Theory and Practice of Histotechnology", second edition,, page 256. By the way, In my first email I did not mean to disparage the use of Lithium Carbonate. Every version of the stain I have seen in the Histology books uses Lithium. So it must work perfectly well in most labs, such as your own. I see my protocol as just an alternative to Lithium just as the Lithium would definitely be an alternative for me if I had problems with the Hydroquinone. Thank you for the detailed description of the Lithium Carbonate technique; I will keep it on hand. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Wed, 17 Jan 2007 10:45:21 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] CD133 Help!! To: "Makhijani, Nalini S" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990D@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii I tried that, but no luck. Did you have some success incubating the primary overnight? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Makhijani, Nalini S [mailto:Nalini.Makhijani@va.gov] Sent: Wednesday, January 17, 2007 8:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 5 Date: Wed, 17 Jan 2007 10:47:38 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] CD133 Help!! To: "Patsy Ruegg" , "Makhijani, Nalini S" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990E@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii I have 3 different CD133/AC133 antibodies. I can't get even one of them to work. I'll have to buy the one you suggest. Thanks Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Wednesday, January 17, 2007 8:36 AM To: 'Makhijani, Nalini S'; Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Which cd133 are you using? If you are using the antibody from Miltyeni I suggest you toss it and get the one from Abcam rab polyclonal ab19898, I use this at 1:100 overnight after HIER steam in HpH buffer for 25 min. with rab labeled polymer hrp detection for 45 min. and dab for 15 min. This antibody as well as many others has been discussed on the NSH IHC Resource Group list serve, to join this group go to www.ihcrg.org and submit a membership form online. We have a list serve similar to histonet that focuses just on IHC/Molecular Diagnostics isssues. Patsy Ruegg Chair, IHCRG -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Makhijani, Nalini S Sent: Wednesday, January 17, 2007 9:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 6 Date: Wed, 17 Jan 2007 11:48:46 -0800 From: "Makhijani, Nalini S" Subject: RE: [Histonet] CD133 Help!! To: "Tarango, Mark" , Message-ID: <715FA772CEA81A47B1A762AB6E45123A2733D7@VHAV22MSGA3.v22.med.va.gov> Content-Type: text/plain; charset="us-ascii" I've never used this antibody- I was just suggesting a general technique that I have seen work with overnight incubation rather than with a 1 hour incubation, e.g.with Pecam 1. -----Original Message----- From: Tarango, Mark [mailto:mtarango@nvcancer.org] Sent: Wednesday, January 17, 2007 10:45 AM To: Makhijani, Nalini S; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! I tried that, but no luck. Did you have some success incubating the primary overnight? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Makhijani, Nalini S [mailto:Nalini.Makhijani@va.gov] Sent: Wednesday, January 17, 2007 8:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== ------------------------------ Message: 7 Date: Wed, 17 Jan 2007 16:02:20 -0500 From: Tim Wheelock Subject: [Histonet] LINES IN PARAFFIN SECTIONS To: Histonet@lists.utsouthwestern.edu Message-ID: <45AE8EDC.7020903@mclean.harvard.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is === message truncated === Scheherazade H. --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From tbraud <@t> holyredeemer.com Thu Jan 18 11:25:54 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 18 11:27:57 2007 Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 26 In-Reply-To: <363901.75989.qm@web81007.mail.mud.yahoo.com> Message-ID: This is a link to a page containing some of the recent publications on blue nevus and its varying forms. http://www.thedoctorsdoctor.com/diseases/bluenevus.html#ipox Since the diagnosis can be malignant to benign, with varying forms in between, microscopic evalutaion with stain correlation is key. Also, because of the various forms, there are no single definitive stains. Good luck with your project. Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scheherazade Humphrey Sent: Thursday, January 18, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 26 Can anyone help me find a IHC stain the is performed on the disease Blue Nevus. I am having a hard time finding out if Fontana Masson is the correct one to use and also a possible case study to reference this disease to. I had to reasearch this disease for a school project. If anyone could help me, your participation would be greatly appreciated. Thank You. histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE:Information on Antibodies (Mildred Fail) 2. EMPLOYMENT OPPROTUNITIES (Jessica Hirsch) 3. Luxol Reference (Tim Wheelock) 4. RE: CD133 Help!! (Tarango, Mark) 5. RE: CD133 Help!! (Tarango, Mark) 6. RE: CD133 Help!! (Makhijani, Nalini S) 7. LINES IN PARAFFIN SECTIONS (Tim Wheelock) 8. Re: LINES IN PARAFFIN SECTIONS (Rene J Buesa) 9. RE: LINES IN PARAFFIN SECTIONS (Weems, Joyce) 10. RE: LINES IN PARAFFIN SECTIONS (Rittman, Barry R) 11. Racemace/p63 cocktail (Dana Settembre) 12. looking for Andra Erickson (Patsy Ruegg) 13. Fwd: Re: [Histonet] LINES IN PARAFFIN SECTIONS (Gayle Callis) 14. LINES IN PARAFFIN SECTIONS 2 (Tim Wheelock) 15. RE: One more thing regarding Luxol Fast Blue differentiator (Dennis Najarian) 16. FW: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Sherwood, Margaret ) 17. Re: LINES IN PARAFFIN SECTIONS (Jan.Minshew@leica-microsystems.com) 18. Re: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Robert Chiovetti) 19. Help with a Reichart Jung Frigocut 2800 (Kurt Musselmann) 20. RE: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Zummak Melissa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 17 Jan 2007 13:01:10 -0500 From: "Mildred Fail" Subject: [Histonet] RE:Information on Antibodies To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 17 Jan 2007 13:20:35 -0500 From: "Jessica Hirsch" Subject: [Histonet] EMPLOYMENT OPPROTUNITIES To: Message-ID: Content-Type: text/plain; charset="us-ascii" CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: ~$30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k 3. Generalist Hours: Monday - Friday, Day Shift Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com ------------------------------ Message: 3 Date: Wed, 17 Jan 2007 13:50:20 -0500 From: Tim Wheelock Subject: [Histonet] Luxol Reference To: Histonet@lists.utsouthwestern.edu Message-ID: <45AE6FEC.3000703@mclean.harvard.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Gayle: As I was mentioning to Andi, I myself cannot find a reference for the protocol. I was taught the stain at the Massachusetts General Hospital back in 1984. (It was simply typed on a 3 x 5 file card) I took a copy of the recipe with me when I left the General in 1986. The neuropathology lab was liquidated some years ago, and it's functions integrated into general pathology. Perhaps, someone in Surgical Pathology or Special Stains at MGH could tract it down. The main number at MGH is 617-726-2000 It is very interesting though that the exact same "differentiator" of the Luxol (1% hydroquinone, 5% sodium sulfite, aqueous) is used for the reducing agent in the Bodian protein-silver stain. We used it for both purposes at the MGH., although I use the Bielschowsky silver stain instead here at McLean One can find find a reference for the Bodian, at least, in Sheehan's "Theory and Practice of Histotechnology", second edition,, page 256. By the way, In my first email I did not mean to disparage the use of Lithium Carbonate. Every version of the stain I have seen in the Histology books uses Lithium. So it must work perfectly well in most labs, such as your own. I see my protocol as just an alternative to Lithium just as the Lithium would definitely be an alternative for me if I had problems with the Hydroquinone. Thank you for the detailed description of the Lithium Carbonate technique; I will keep it on hand. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Wed, 17 Jan 2007 10:45:21 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] CD133 Help!! To: "Makhijani, Nalini S" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990D@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii I tried that, but no luck. Did you have some success incubating the primary overnight? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Makhijani, Nalini S [mailto:Nalini.Makhijani@va.gov] Sent: Wednesday, January 17, 2007 8:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 5 Date: Wed, 17 Jan 2007 10:47:38 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] CD133 Help!! To: "Patsy Ruegg" , "Makhijani, Nalini S" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F990E@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii I have 3 different CD133/AC133 antibodies. I can't get even one of them to work. I'll have to buy the one you suggest. Thanks Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Wednesday, January 17, 2007 8:36 AM To: 'Makhijani, Nalini S'; Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Which cd133 are you using? If you are using the antibody from Miltyeni I suggest you toss it and get the one from Abcam rab polyclonal ab19898, I use this at 1:100 overnight after HIER steam in HpH buffer for 25 min. with rab labeled polymer hrp detection for 45 min. and dab for 15 min. This antibody as well as many others has been discussed on the NSH IHC Resource Group list serve, to join this group go to www.ihcrg.org and submit a membership form online. We have a list serve similar to histonet that focuses just on IHC/Molecular Diagnostics isssues. Patsy Ruegg Chair, IHCRG -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Makhijani, Nalini S Sent: Wednesday, January 17, 2007 9:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 6 Date: Wed, 17 Jan 2007 11:48:46 -0800 From: "Makhijani, Nalini S" Subject: RE: [Histonet] CD133 Help!! To: "Tarango, Mark" , Message-ID: <715FA772CEA81A47B1A762AB6E45123A2733D7@VHAV22MSGA3.v22.med.va.gov> Content-Type: text/plain; charset="us-ascii" I've never used this antibody- I was just suggesting a general technique that I have seen work with overnight incubation rather than with a 1 hour incubation, e.g.with Pecam 1. -----Original Message----- From: Tarango, Mark [mailto:mtarango@nvcancer.org] Sent: Wednesday, January 17, 2007 10:45 AM To: Makhijani, Nalini S; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! I tried that, but no luck. Did you have some success incubating the primary overnight? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Makhijani, Nalini S [mailto:Nalini.Makhijani@va.gov] Sent: Wednesday, January 17, 2007 8:08 AM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD133 Help!! Try letting it go overnight. Nalini Makhijani -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, January 16, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD133 Help!! If anyone has a protocol for CD133 on paraffin OR frozen tissue that works, please send it to me! I've tried so many times w/ this antibody and I'm having a hell of a time getting any staining. Thanks!! Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== ------------------------------ Message: 7 Date: Wed, 17 Jan 2007 16:02:20 -0500 From: Tim Wheelock Subject: [Histonet] LINES IN PARAFFIN SECTIONS To: Histonet@lists.utsouthwestern.edu Message-ID: <45AE8EDC.7020903@mclean.harvard.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Everyone: I have a serious problem with lines/splits forming in my 5 micron sections as they come off the microtome. I just started using Paraplast Xtra Tissue Embedding Medium. My infiltrating wax is regular Paraplast. My disposable blades are Surgipath high profile Teflon coated blades. The microtome is a Microm HM 355S. I cut human brain tissue exclusively. I had been using Surgipath embedding media. I decided to try the X-tra because I had been having problems with the sections being thick and thin on just one side of the section. Also the wax seeming to be thicker (more opaque) on one side of the block. The blocks of tissue also were not laying flat in the mold, hence a lot of trimming after. There was some problem with lines even with the Surgipath but it was intermittent., and not nearly as bad as the X-tra. I notice that trimming requires more physical effort with the Paraplast X-tra. And I have had much more of a problem with lines than with the Surgipath Embedding Media. Does it have a higher % of plastic in it. I cleaned out the reservoir on the embedding center and played with the knife angle but to no avail. I am not sure if the lines have to do with the wax or the blades or both. Thank you for any advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital I Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is === message truncated === Scheherazade H. --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From Lchausse <@t> nmh.org Thu Jan 18 11:46:43 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Thu Jan 18 11:47:12 2007 Subject: [Histonet] Microwave procedure for monitoringtemperature reproducibility Message-ID: The specific CAP req ANP.27720 which requires annual monitoring for microwave leakage was deleted effective 12/12/2006. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: Thursday, January 18, 2007 9:24 AM To: Zummak Melissa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave procedure for monitoringtemperature reproducibility Hello everyone: And now a vendor weighs in: We have several calibration and "accreditation-related" articles available on our website (www.ebsciences.com , http://www.ebsciences.com/papers/microwave_quality.htm , http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ). In addition, we're happy to see that microwave use in the histology laboratory is finally being given the scrutiny it deserves. Many of the suggestions in CLSI's GP-28 guidelines (referenced in other postings) are excellent, for example, troubleshooting, container placement, power output determination, and overall, standardization of procedures and elimination of variables. However, I feel there are some significant problems with this publication; I have previously informed CLSI of my primary concern: Page 11, section 4.7: "A qualified authority CERTIFIED IN USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine microwave leakage..." The problem is, to our knowledge, NO SUCH CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has not been forthcoming with suggestions. This is not to imply the absence of extant legislation, Federal and otherwise, regulating the manufacture and use of microwave devices; manufacturers have to comply with FDA and other regulations regarding microwaves intended for in-vitro diagnostic use. But as for leakage detection in the field, we have yet to come across any relevant (or irrelevant, for that matter) Federal or State certification. As you can imagine, for institutions deciding to adopt the more stringent CLSI guidelines, this poses something of a "Catch-22." In addition, page 17 outlines the use of a neon bulb array, for those who aspire to eliminate "hot spots" in the microwave chamber. However, while this can be a "fun" way to indirectly observe microwave energy, unless the microwave were horribly defective, it is a fairly useless exercise. Any object (including the array itself) placed in the microwave chamber affects the distribution of microwaves, a classic example of attempted observation affecting the phenomenon observed. I want to stress, however, that overall, publication GP-28A is an outstanding effort to provide "recommendations for quality assurance and safety procedures for microwave equipment use" in their words, and to help troubleshoot and eliminate variability in results of microwave usage. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you. Zummak Melissa wrote: > Hi everyone, > > Does anyone know where I can find recommendations for > checking\monitoring temperature reproducibility for microwaves, I only > use the microwave for certain special stains currently. > > Melissa Zummak > Alverno Clinincal Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From John.MacDougall <@t> ipi.com Thu Jan 18 12:04:11 2007 From: John.MacDougall <@t> ipi.com (John MacDougall) Date: Thu Jan 18 12:04:30 2007 Subject: [Histonet] Trying to get consensus - Frozen section immediate post fixation or air dry Message-ID: <781E27B31A89A040BC9AEE38D04AF42E5A38F0@ipimail02.infinitypharm1.com> Hi Histonet I have seen two basic camps regarding the treatment of frozen sections once the section hits the slide - One camp fixes immediately and one camp leaves the section to air dry, sometimes overnight. I realize that there is likely not a "right" answer, but are there advantages to one process method over another? I'm in the midst of trying out both processes and evaluating myself, but in the meantime I'd like to hear feedback from the community. Thanks John John MacDougall, Ph.D. Lead Senior Scientist Infinity Pharmaceuticals 780 Memorial Drive Cambridge, MA 02139 Voice - 617.453.1214 Fax - 617.453.1001 This message and any attachments may contain confidential information and/or privileged material. If you received this message in error, please contact the sender and delete this message and any attachments immediately. Thank you. From gcallis <@t> montana.edu Thu Jan 18 12:21:37 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jan 18 12:21:41 2007 Subject: [Histonet] Trying to get consensus - Frozen section immediate post fixation or air dry In-Reply-To: <781E27B31A89A040BC9AEE38D04AF42E5A38F0@ipimail02.infinityp harm1.com> References: <781E27B31A89A040BC9AEE38D04AF42E5A38F0@ipimail02.infinitypharm1.com> Message-ID: <6.0.0.22.1.20070118111408.01b44af0@gemini.msu.montana.edu> John, How you treat a frozen section is going to depend on what you are planning to do with the frozen section. If you can be more specific that will help. Do you want to immunohistochemical staining on a snap frozen tissue? Do you want to fix and then store these frozen sections for immunohistochemistry? Do you want to store an unfixed frozen section for future immunohistochemical staining? Do you want to do a rapid H&E for diagnostic purposes? We do any one of the above depending on a particular staining procedure needed. At 11:04 AM 1/18/2007, you wrote: >Hi Histonet > >I have seen two basic camps regarding the treatment of frozen sections >once the section hits the slide - One camp fixes immediately and one >camp leaves the section to air dry, sometimes overnight. I realize that >there is likely not a "right" answer, but are there advantages to one >process method over another? I'm in the midst of trying out both >processes and evaluating myself, but in the meantime I'd like to hear >feedback from the community. > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From tbraud <@t> holyredeemer.com Thu Jan 18 12:25:38 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 18 12:27:40 2007 Subject: [Histonet] Trying to get consensus - Frozen section immediate postfixation or air dry In-Reply-To: <781E27B31A89A040BC9AEE38D04AF42E5A38F0@ipimail02.infinitypharm1.com> Message-ID: One person's opinion It depends on the application for me, but generally, if morphology is important, then I fix them right away. Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of John MacDougall Sent: Thursday, January 18, 2007 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trying to get consensus - Frozen section immediate postfixation or air dry Hi Histonet I have seen two basic camps regarding the treatment of frozen sections once the section hits the slide - One camp fixes immediately and one camp leaves the section to air dry, sometimes overnight. I realize that there is likely not a "right" answer, but are there advantages to one process method over another? I'm in the midst of trying out both processes and evaluating myself, but in the meantime I'd like to hear feedback from the community. Thanks John John MacDougall, Ph.D. Lead Senior Scientist Infinity Pharmaceuticals 780 Memorial Drive Cambridge, MA 02139 Voice - 617.453.1214 Fax - 617.453.1001 This message and any attachments may contain confidential information and/or privileged material. If you received this message in error, please contact the sender and delete this message and any attachments immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From Lchausse <@t> nmh.org Thu Jan 18 12:37:46 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Thu Jan 18 12:38:17 2007 Subject: [Histonet] Ventana IHC Staining Message-ID: We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From DGNajarian <@t> comcast.net Thu Jan 18 12:39:28 2007 From: DGNajarian <@t> comcast.net (Dennis Najarian) Date: Thu Jan 18 12:39:43 2007 Subject: [Histonet] UNSUBSCRIBE Message-ID: Please unsubscribe. Thanks. From rjbuesa <@t> yahoo.com Thu Jan 18 12:46:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 18 12:53:05 2007 Subject: [Histonet] Ventana IHC Staining In-Reply-To: Message-ID: <20070118184615.33487.qmail@web61223.mail.yahoo.com> Leslie: I suggest you to go to Histonet Archieves and find out about this very same recurrent problem with the Ventana Benchmark (and a very large and controversial thread about the likes and dislikes about Ventana). I personally do not like closed systems for IHC, I always used and preferred Dako and cannot help you on this problem. Check the Archieves! Ren? J. "Chaussey, Leslie" wrote: We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From Anna.Inman <@t> stmarygj.org Thu Jan 18 13:00:17 2007 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Thu Jan 18 13:00:34 2007 Subject: [Histonet] Small Bx protocol ? Message-ID: <2925AE271EAAD440AF48FCCEB8002D090372F4C5@smgmail01.smgj.sclhs.net> What protocol is being used for tissue processors that only contain "small" biopsies? We have Tissue Tek VIP's if it matters... Thank you in advance Anna Inman, HT(ASCP) Clinical Resource Specialist SMH Pathology Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jmahoney <@t> alegent.org Thu Jan 18 13:12:06 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Jan 18 13:13:39 2007 Subject: [Histonet] Ventana IHC Staining In-Reply-To: <20070118184615.33487.qmail@web61223.mail.yahoo.com> References: <20070118184615.33487.qmail@web61223.mail.yahoo.com> Message-ID: <45AF72260200003C00002824@gwia.alegent.org> Oh Boy, here we go again. I'm a Ventana user and when we have had this occasional problem it has been something simple like needing to do the routine maintenance. Have you performed your function tests, checked your heat pads and decontaminated your instrument? I also have found the Ventana customer service people helpful. Hope this helps you solve your problem. Jan Omaha >>> Rene J Buesa 01/18/2007 12:46 PM >>> Leslie: I suggest you to go to Histonet Archieves and find out about this very same recurrent problem with the Ventana Benchmark (and a very large and controversial thread about the likes and dislikes about Ventana). I personally do not like closed systems for IHC, I always used and preferred Dako and cannot help you on this problem. Check the Archieves! Ren? J. "Chaussey, Leslie" wrote: We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Jan 18 13:17:27 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Jan 18 13:15:00 2007 Subject: [Histonet] Ventana IHC Staining In-Reply-To: Message-ID: A number of things can cause inconsistent staining. Here are a few. Barcode label properties Altered 1) The hydrophobic properties of the labels can cause nonstaining of the issue if it is placed to close to the label. 2) Placement of the labels on the slides-Crooked labels can cause the solutions to wick off the edge of the slides. 3) Cutting the Labels edges-If the labels are cut it increases the slides surface area and can cause light staining. 4) Aging-The glue can get old Silanization of Glass Slides 1) Making of a charged glass slide. Different Vendors have different quality. 2) Consider factors that compromise the adhesion: A. "Double dipping of slides". This is where the control is picked up first, then at a later time the patient is picked up and the slide is wet twice. B. "Stay-On" used with plus slides. 3) Consider the factors that produce unwanted background. A. Gelatin in the waterbath. B. Elmer's Glue in the waterbath. 4) Check the storage conditions, age and lot numbers of the slides. If these steps don't help then do a Vortex Mix test. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chaussey, Leslie Sent: Thursday, January 18, 2007 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana IHC Staining We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wlove <@t> att.net Thu Jan 18 13:17:24 2007 From: wlove <@t> att.net (wlove@att.net) Date: Thu Jan 18 13:17:34 2007 Subject: [Histonet] Re: Microwave procedure for monitoring temperature Message-ID: <011820071917.20643.45AFC7C3000E97DE000050A321603763160A90010499@att.net> Hello, we are a company that designs, deveolps and produces laboratory and industrial microwaves. Between my partner and myself, we have over 50 years experience in microwave equipment used in heating materials, like laboratory microwaves (commonly called ISM equipment). The issue of microwave leakage, their acceptable levels and how/who to measure them has been problematic for the last 20 years or so. Different limits apply to different countries and in some cases individual states in the US. Who tests for the leakage is less important than the type of meter used and under what conditions the test where performed. First be sure to use a microwave leakage meter designed to read microwave fields at 2.45 GHz. Furthermore, the meter should be calibrated and traceable to NIST (US National Institute of Standards and Technology). All of the small cheap meters not calibrated and tracable to NIST we evaluated have given both false positive and negative readings compared to those that are calibrated and tracable to NIST. No matter if the meter operator is trained or certified, if he/she is using an uncalibrated or without tracability to NIST, any readings taken are likely faulty. Next, the load in the microwave should be small as large loads adsorb microwave energy and therefore prevent the microwave energy from leaking from the microwave, hence another potential source of error. Microwave leakage is statistical, meaning just do not do one test and assume everything is OK. Microwave leakage can vary with time (due to the turntable or mode stirrer position, load and the phase of the line cycle at the time of measurement at that instant.). So the test should be repeated more than once for more valid testing. I believe CLSI GP-28A, the standard for using a microwave in the laboratory was written by a number of people working to develop a guideline. As a member of IMPI, International Microwave Power Institute and former board member and section president, to the best of my knowledge the group that developed GP-28A did not contact IMPI for guidance, nor have I heard of anyone whom I consider to be in the ISM community to be contacted. I was able to unofficially read a draft as one of the members was looking for feedback, but this was well after they approved the draft. I agree with Phil McArdle of EB Sciences that much of GP-28A is very good and useful, but there are some serious issues that should be corrected. In the meantime, only calibrated and traceable to NIST leakage meters should be used multiple times to measure leakage. Best regards Wayne Love Microwave Research and Applications, Inc. 8673 Cherry Lane Laurel, MD 20707 Phone 301-953-1771 Fax 301-369-0523 WebSite www.microwaveresearch.com Email info@microwaveresearch.com Cell 630-269-5158 Direct email: wlove@att.net From mrrholgado <@t> yahoo.com Thu Jan 18 13:13:22 2007 From: mrrholgado <@t> yahoo.com (rosadel holgado) Date: Thu Jan 18 13:24:05 2007 Subject: [Histonet] project on microwave Message-ID: <20070118191322.74944.qmail@web37414.mail.mud.yahoo.com> Hi! I am a newbie here and I wonder if it's appropriate to be asking questions here about my project. I am planning to do a project for my MSc soon and I was thinking of doing something about microwave fixation and microwave assisted processing. Does anyone know of a protocol I can cite for using a domestic microwave for the fixation? Thanks! Rosadel Salita Histology Surrey, England From doug <@t> ppspath.com Thu Jan 18 13:46:32 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Jan 18 13:43:59 2007 Subject: [Histonet] Ventana IHC Staining In-Reply-To: <20070118184615.33487.qmail@web61223.mail.yahoo.com> Message-ID: If I recall correctly, Leslie asked for help with the Benchmark. I don't think that she asked for an opinion and for someone to say that that they cannot help. Maybe I am mistaken? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 18, 2007 1:46 PM To: Chaussey, Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana IHC Staining Leslie: I suggest you to go to Histonet Archieves and find out about this very same recurrent problem with the Ventana Benchmark (and a very large and controversial thread about the likes and dislikes about Ventana). I personally do not like closed systems for IHC, I always used and preferred Dako and cannot help you on this problem. Check the Archieves! Ren? J. "Chaussey, Leslie" wrote: We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Derek.Papalegis <@t> tufts.edu Thu Jan 18 13:44:43 2007 From: Derek.Papalegis <@t> tufts.edu (Derek Papalegis) Date: Thu Jan 18 13:44:52 2007 Subject: [Histonet] varistain heat stations Message-ID: <20070118144443.jmxvoutockc84c84@webmail.tufts.edu> I have a question about thermo's veristain gemini stainer. can the heat stations be used in place of an oven? We are trying to save money on equipment and havent found an inexpensive slide drying oven so if we could just use the heat stations, that would work out well. does anyone know where small, relatively inexpensive slide ovens can be purchased at? thanks, derek From rjbuesa <@t> yahoo.com Thu Jan 18 13:51:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 18 13:52:05 2007 Subject: [Histonet] Small Bx protocol ? In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D090372F4C5@smgmail01.smgj.sclhs.net> Message-ID: <20070118195156.61079.qmail@web61224.mail.yahoo.com> My "standard" protocol for small bx. was completed in 2h40min meaning that it should be short, specially in the dehydration steps. I always used Sakura's VIPs. Ren? J. "Inman, Anna" wrote: What protocol is being used for tissue processors that only contain "small" biopsies? We have Tissue Tek VIP's if it matters... Thank you in advance Anna Inman, HT(ASCP) Clinical Resource Specialist SMH Pathology Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From JEllin <@t> yumaregional.org Thu Jan 18 13:53:48 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Jan 18 13:58:10 2007 Subject: [Histonet] Ventana IHC Staining Message-ID: <29BE166A2CF48D459853F8EC57CD37E87E9C4C@EXCHANGECLUSTER.yumaregional.local> To me it sounds that you need to get your mixers checked and make sure that they are not clogged. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, January 18, 2007 12:12 PM To: histonet@pathology.swmed.edu; Rene J Buesa Subject: Re: [Histonet] Ventana IHC Staining Oh Boy, here we go again. I'm a Ventana user and when we have had this occasional problem it has been something simple like needing to do the routine maintenance. Have you performed your function tests, checked your heat pads and decontaminated your instrument? I also have found the Ventana customer service people helpful. Hope this helps you solve your problem. Jan Omaha >>> Rene J Buesa 01/18/2007 12:46 PM >>> Leslie: I suggest you to go to Histonet Archieves and find out about this very same recurrent problem with the Ventana Benchmark (and a very large and controversial thread about the likes and dislikes about Ventana). I personally do not like closed systems for IHC, I always used and preferred Dako and cannot help you on this problem. Check the Archieves! Ren? J. "Chaussey, Leslie" wrote: We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From arvidsonkristen <@t> yahoo.com Thu Jan 18 14:12:50 2007 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Thu Jan 18 14:19:39 2007 Subject: [Histonet] Microwave Processors Message-ID: <304929.95462.qm@web61321.mail.yahoo.com> Hi all, I am inquiring once again about the microwave tissue processors. I wanted to know some specific info if anyone had it. Does anyone do skin? How are the fatty tissues? Quality? Reagents...do you have to buy them directly from the processor company? Cost of reagents compared to the regular ones? Service and maintenance? Specific brands people use. How does it effect tech scheduling? Sorry this seems so random...Thanks for all your help! -Kristen --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From schraz13 <@t> yahoo.com Thu Jan 18 14:29:13 2007 From: schraz13 <@t> yahoo.com (Scheherazade Humphrey) Date: Thu Jan 18 14:29:28 2007 Subject: [Histonet] Blue Nevus Diease-Help? Message-ID: <931881.22835.qm@web81001.mail.mud.yahoo.com> Sorry I forgot to change the subject line for my message. Can anyone help me find a IHC stain the is performed on the disease Blue Nevus. I am having a hard time finding out if Fontana Masson is the correct one to use and also a possible case study to reference this disease to. I had to reasearch this disease for a school project. If anyone could help me, your participation would be greatly appreciated. Thank You. histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE:Information on Antibodies (Mark Elliott) 2. CK 19 (Dana Settembre) 3. Re: Help with a Reichert Jung Frigocut 2800 (Don.Birgerson@leica-microsystems.com) 4. RE: Microwave procedure for monitoring temperature rep roducibility (hymclab) 5. Re: Microwave procedure for monitoring temperature reproducibility (Phil McArdle) 6. RE: Microwave procedure for monitoring temperature rep roducibility (Inman, Anna) 7. RE: Microwave procedure for monitoringtemperature reproducibility (Morken, Tim) 8. Re: Histonet Digest, Vol 38, Issue 26 (Scheherazade Humphrey) 9. RE: Re: Histonet Digest, Vol 38, Issue 26 (Terri Braud) 10. RE: Microwave procedure for monitoringtemperature reproducibility (Chaussey, Leslie) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 Jan 2007 06:21:32 -0800 From: "Mark Elliott" Subject: [Histonet] RE:Information on Antibodies To: , Message-ID: <45AF11EC020000D6000306CE@mail.mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII Marsha I have used D2-40. What sort of info are you looking for? MArk >>> "Mildred Fail" 1/17/2007 10:01:10 AM >>> Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ------------------------------ Message: 2 Date: Thu, 18 Jan 2007 09:36:28 -0500 From: "Dana Settembre" Subject: [Histonet] CK 19 To: , , "Mark Elliott" Message-ID: Content-Type: text/plain; charset=US-ASCII Hello Marsha, I use CK 19 on FFPE human tissue. I use Dako's cat.# M0888 It is an In Vitro Diagnostic antibody. I use it at a dilution of 1:25 and incubate 15 mnutes RT I also use their Target Retreival Solution and use a papillary carcinoma as a positive control. I get nice results. Good Luck Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Mark Elliott 01/18/07 9:21 AM >>> Marsha I have used D2-40. What sort of info are you looking for? MArk >>> "Mildred Fail" 1/17/2007 10:01:10 AM >>> Marsha We use 2. Mesothelial clone HBME-1 from DAKO IVD AB 3. CK 19 clone b170 fromVector RUO Ab Rena Fail >>> "mprice26@juno.com" 01/17/07 12:15PM >>> Hello in Histoland, I need information on the following antibodies: 1. D2-40 2. HBME-1 3. CK-19 Thank you in advance. Marsha Price, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 18 Jan 2007 08:44:44 -0600 From: Don.Birgerson@leica-microsystems.com Subject: Re: [Histonet] Help with a Reichert Jung Frigocut 2800 To: Kurt Musselmann Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hello Kurt, I believe we still have anti roll plates for most of our cryostats. I will call you on the phone so we can play a game of twenty questions to identify your holder. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 (7:00 to 4:00 CT) Kurt Musselmann .nih.gov> To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Help with a Reichart Jung Frigocut 2800 01/18/2007 08:06 AM Hello everybody I am new to the list, and was directed to you guys by Alan Bright. I have started using a Reichart Jung Frigocut 2800, and we have a badly chipped anti-roll plate. Our plate measures roughly 5x3x0.3 cm, and I am trying to find a replacement for it. Does anybody know of a company that manufactures anti-roll plates of that size, or would I have to change the whole anti-roll device (I had ordered one from Electron Microscope Sciences (EMS) but it did not fit our machine and had to be returned. I appreciate any input Kurt Musselmann -- Kurt Musselmann Ph.D. NIDCR/NIH 30 Convent Drive Building 30, Room 403 Bethesda, MD 20892-4370 (301) 496 3065 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 4 Date: Thu, 18 Jan 2007 08:51:49 -0600 From: hymclab Subject: RE: [Histonet] Microwave procedure for monitoring temperature rep roducibility To: 'Zummak Melissa' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain There is a CLSI (formerly NCCLS) Standard and Guideline book available: Microwave Device Use in the Histology Laboratory; Approved Guidelines. (description: This document provides recommendations for reproducing the performance of microwave-accelereated procedures to prepare biological specimens in the histology laboratory). Go to the CLSI website for info: www.clsi.org. I found this very helpful in fulfilling the new CAP requirements. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Zummak Melissa [mailto:Melissa.Zummak@ssfhs.org] Sent: Wednesday, January 17, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility Hi everyone, Does anyone know where I can find recommendations for checking\monitoring temperature reproducibility for microwaves, I only use the microwave for certain special stains currently. Melissa Zummak Alverno Clinincal Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ Message: 5 Date: Thu, 18 Jan 2007 10:24:00 -0500 From: Phil McArdle Subject: Re: [Histonet] Microwave procedure for monitoring temperature reproducibility To: Zummak Melissa Cc: histonet@lists.utsouthwestern.edu Message-ID: <45AF9110.8080104@ebsciences.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello everyone: And now a vendor weighs in: We have several calibration and "accreditation-related" articles available on our website (www.ebsciences.com , http://www.ebsciences.com/papers/microwave_quality.htm , http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ). In addition, we're happy to see that microwave use in the histology laboratory is finally being given the scrutiny it deserves. Many of the suggestions in CLSI's GP-28 guidelines (referenced in other postings) are excellent, for example, troubleshooting, container placement, power output determination, and overall, standardization of procedures and elimination of variables. However, I feel there are some significant problems with this publication; I have previously informed CLSI of my primary concern: Page 11, section 4.7: "A qualified authority CERTIFIED IN USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine microwave leakage..." The problem is, to our knowledge, NO SUCH CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has not been forthcoming with suggestions. This is not to imply the absence of extant legislation, Federal and otherwise, regulating the manufacture and use of microwave devices; manufacturers have to comply with FDA and other regulations regarding microwaves intended for in-vitro diagnostic use. But as for leakage detection in the field, we have yet to come across any relevant (or irrelevant, for that matter) Federal or State certification. As you can imagine, for institutions deciding to adopt the more stringent CLSI guidelines, this poses something of a "Catch-22." In addition, page 17 outlines the use of a neon bulb array, for those who aspire to eliminate "hot spots" in the microwave chamber. However, while this can be a "fun" way to indirectly observe microwave energy, unless the microwave were horribly defective, it is a fairly useless exercise. Any object (including the array itself) placed in the microwave chamber affects the distribution of microwaves, a classic example of attempted observation affecting the phenomenon observed. I want to stress, however, that overall, publication GP-28A is an outstanding effort to provide "recommendations for quality assurance and safety procedures for microwave equipment use" in their words, and to help troubleshoot and eliminate variability in results of microwave usage. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you. Zummak Melissa wrote: > Hi everyone, > > Does anyone know where I can find recommendations for > checking\monitoring temperature reproducibility for microwaves, I only > use the microwave for certain special stains currently. > > Melissa Zummak > Alverno Clinincal Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ------------------------------ Message: 6 Date: Thu, 18 Jan 2007 08:50:47 -0700 From: "Inman, Anna" Subject: RE: [Histonet] Microwave procedure for monitoring temperature rep roducibility To: "hymclab" , "Zummak Melissa" , Message-ID: <2925AE271EAAD440AF48FCCEB8002D090372F4C3@smgmail01.smgj.sclhs.net> Content-Type: text/plain; charset="us-ascii" What I recall about that publication......it doesn't really address microwave usage for anything other than a specimen processing angle; whereas, labs like mine only use it for heating agar/histogel to make cell blocks and things........ Anna Inman, HT(ASCP) Clinical Resource Specialist SMH Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, January 18, 2007 7:52 AM To: 'Zummak Melissa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave procedure for monitoring temperature rep roducibility There is a CLSI (formerly NCCLS) Standard and Guideline book available: Microwave Device Use in the Histology Laboratory; Approved Guidelines. (description: This document provides recommendations for reproducing the performance of microwave-accelereated procedures to prepare biological specimens in the histology laboratory). Go to the CLSI website for info: www.clsi.org. I found this very helpful in fulfilling the new CAP requirements. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Zummak Melissa [mailto:Melissa.Zummak@ssfhs.org] Sent: Wednesday, January 17, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave procedure for monitoring temperature reproducibility Hi everyone, Does anyone know where I can find recommendations for checking\monitoring temperature reproducibility for microwaves, I only use the microwave for certain special stains currently. Melissa Zummak Alverno Clinincal Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 7 Date: Thu, 18 Jan 2007 11:33:53 -0500 From: "Morken, Tim" Subject: RE: [Histonet] Microwave procedure for monitoringtemperature reproducibility To: Message-ID: Content-Type: text/plain; charset="us-ascii" Phil wrote: "Page 11, section 4.7: "A qualified authority CERTIFIED IN USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine microwave leakage..." The problem is, to our knowledge, NO SUCH CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has not been forthcoming with suggestions." That's funny, but brings up an interesting question: Who does oversight on a group like CLSI? If they went to the trouble of researching this guideline, how is it that they never (apparently) realized there is no such certification. What made them write such a requirement without knowing if such a certification exisits? Did they just assume it existed? And, if they did any lab work with an actual microwave, how could they have done so without first having it checked by a person certified to do so? The final question. If CLSI can't figure that out, should we believe them on anything else? This is a private group that has set itself up as the final word on laboratory standardization. They charge massive amounts of money for rather average informational documents (most of which, these days, anyone can find on the internet for free with a little persistance) and then something like this makes them look like fools. It seems the only possible oversight is to refuse to buy defective documents. Actually, it kinda reminds me of another body that dreams up fantasy questions to torture lab managers with... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: Thursday, January 18, 2007 7:24 AM To: Zummak Melissa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave procedure for monitoringtemperature reproducibility Hello everyone: And now a vendor weighs in: We have several calibration and "accreditation-related" articles available on our website (www.ebsciences.com , http://www.ebsciences.com/papers/microwave_quality.htm , http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ). In addition, we're happy to see that microwave use in the histology laboratory is finally being given the scrutiny it deserves. Many of the suggestions in CLSI's GP-28 guidelines (referenced in other postings) are excellent, for example, troubleshooting, container placement, power output determination, and overall, standardization of procedures and elimination of variables. However, I feel there are some significant === message truncated === Scheherazade H. --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From rjbuesa <@t> yahoo.com Thu Jan 18 14:07:41 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 18 14:38:25 2007 Subject: [Histonet] project on microwave In-Reply-To: <20070118191322.74944.qmail@web37414.mail.mud.yahoo.com> Message-ID: <20070118200741.95750.qmail@web61219.mail.yahoo.com> Hi Rosalde Holgado: Word of caution with your MSc project: I do not think it will be accepted because it is already common knowledge that domestic microwaves should NOT be used for any task other than the ones they have been designed for, meaning: heating food in the home setting. If you want to pursue this type of project you should try to obtain a laboratory designed MWO and focus your thesis as an "evaluation" project, where you will process tissues with the MWO and compare the results with those obtained with parallel samples using a conventional tissue processor. This is always interesting and you may even try to get the MWO from a manufacturer interested in such validations, since they are (except for some Milestone and Sakura instruments) inexistent at this moment. Hope this will help you! Ren? J. rosadel holgado wrote: Hi! I am a newbie here and I wonder if it's appropriate to be asking questions here about my project. I am planning to do a project for my MSc soon and I was thinking of doing something about microwave fixation and microwave assisted processing. Does anyone know of a protocol I can cite for using a domestic microwave for the fixation? Thanks! Rosadel Salita Histology Surrey, England _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From HoustonR <@t> chi.osu.edu Thu Jan 18 14:39:40 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Thu Jan 18 14:40:10 2007 Subject: [Histonet] SEM for hair shaft analysis Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20C2DD21E@chi2k3ms01.columbuschildrens.net> Does anyone know where, in the US, we can get SEM done for hair-shaft analysis in alopecia? Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From pmcardle <@t> ebsciences.com Thu Jan 18 14:53:34 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Thu Jan 18 14:53:44 2007 Subject: [Histonet] project on microwave In-Reply-To: <20070118191322.74944.qmail@web37414.mail.mud.yahoo.com> References: <20070118191322.74944.qmail@web37414.mail.mud.yahoo.com> Message-ID: <45AFDE4E.3000906@ebsciences.com> Hello: A microwave vendor weighs in: EBS' position is that domestic microwaves not be used for lab work, but then, we're in the position of manufacturing lab microwaves. :-) That said, in this case I can understand the temptation to do so, given the academic context and undoubtedly inherent budget constraints. I would be careful, however: of all the operations lab microwaves can be used for, fixation and tissue processing are the most demanding and require the tightest temperature control. Besides the issue of a temperature probe (most domestic microwaves don't have one) there is the issue of magnetron cycle time; domestic microwaves' cycle times are typically too long to allow the temperature control required for small, delicate histological samples. The chances of cooking your tissue are great. I'd explore other options: local locations with laboratory microwaves who might let you into their lab. Especially for fixation and processing, a true laboratory microwave is the surest path to success. Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you. rosadel holgado wrote: > Hi! > > I am a newbie here and I wonder if it's appropriate to be asking questions here about my project. I am planning to do a project for my MSc soon and I was thinking of doing something about microwave fixation and microwave assisted processing. Does anyone know of a protocol I can cite for using a domestic microwave for the fixation? > > Thanks! > > Rosadel Salita > Histology > Surrey, England > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Heidi.Miers <@t> NAU.EDU Thu Jan 18 15:18:05 2007 From: Heidi.Miers <@t> NAU.EDU (Heidi Miers) Date: Thu Jan 18 15:18:18 2007 Subject: [Histonet] Rapid H &E Message-ID: <45AFE40D.5090003@nau.edu> I am new to this field and am curious about the rapid H & E. How is it performed and what are the results like? Thanks, Heidi Heidi Miers Research Specialist, Sr. Imaging and Histology Core Facility Discovery Research Laboratories Northern Arizona University Heidi.Miers@nau.edu 928-523-9422 From oshel1pe <@t> cmich.edu Thu Jan 18 15:16:22 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Jan 18 15:20:38 2007 Subject: [Histonet] SEM for hair shaft analysis In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB20C2DD21E@chi2k3ms01.columbuschildrens.net > References: <979FF5962E234F45B06CF0DB7C1AABB20C2DD21E@chi2k3ms01.columbuschildrens.net > Message-ID: What kind of analysis? Imaging? EDS? ... ? We do hairs, among other things, but if you want analysis of trace elements, SEM/EDS likely won't be sensitive enough. Phil >Does anyone know where, in the US, we can get SEM done for >hair-shaft analysis in alopecia? > >Thanks >Ronnie > >Ronnie Houston, MS, HT(ASCP)QIHC >Anatomic Pathology Manager >Columbus Children's Hospital >700 Children's Drive >Columbus, OH 43205 >(614) 722 5465 >houstonr@chi.osu.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From Jackie.O'Connor <@t> abbott.com Thu Jan 18 15:29:09 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jan 18 15:30:26 2007 Subject: [Histonet] Vital stain for mouse v. human cells In-Reply-To: <45AFDE4E.3000906@ebsciences.com> Message-ID: Earlier today I think I read a post from someone about staining only mouse cells in a human xenograft, and of course, I deleted it instead of saving it. Would someone please give me the general gist of how someone is staining only mouse cells? I thought it was a vital stain of some sort. thanks Jackie O' From liz <@t> premierlab.com Thu Jan 18 15:57:23 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 18 15:48:42 2007 Subject: [Histonet] Vital stain for mouse v. human cells In-Reply-To: Message-ID: <000201c73b4b$a25cdf90$0d00a8c0@domain.Premier> Jackie I have not heard of a vital stain, but I know that this can be determined via in-situ hybridization. You can stain for mouse satellite DNA repeat sequences, you need to get an oligo probe that is specific to the mouse genome and will not detect human. We have one that we have used for mouse verses porcine tissue. We get our probes from Genedetect. I also have a few papers on this if you would like them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, January 18, 2007 2:29 PM To: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Vital stain for mouse v. human cells Earlier today I think I read a post from someone about staining only mouse cells in a human xenograft, and of course, I deleted it instead of saving it. Would someone please give me the general gist of how someone is staining only mouse cells? I thought it was a vital stain of some sort. thanks Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From kmilne <@t> bccancer.bc.ca Thu Jan 18 16:06:23 2007 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Jan 18 16:06:32 2007 Subject: [Histonet] Double staining with Mouse Mabs on Ventana discovery Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780240D2E0@srvex03.phsabc.ehcnet.ca> Hi Everyone, I'm wondering if anyone has experience double-staining using 2 mouse monoclonal antibodies (IHC, one for CD4, one for FoxP3) on human FFPE tissue using the Ventana Discovery system? Do you have any protocols you could share? Also, I already have the DAB-MAP kit in house and would like to use that in the detection rather than buying 2 new detection kits, which Ventana detection kit should I use for best contrast and practicality (slides mounted using Cytoseal 60 following alcohol and xylene). Any suggestions would be much appreciated! Katy Milne Deeley Research Centre BC Cancer Agency From JMacDonald <@t> mtsac.edu Thu Jan 18 17:24:51 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 18 17:25:00 2007 Subject: [Histonet] Sudan IV staining Message-ID: A researcher at an area hospital has asked for some help performing Sudan IV staining of rat aortas. She would like to look for atherosclerotic lesions by en face analysis. I am not familiar with this. I have reviewed the paper she is using as a reference and it does not go into detail about the staining protocol for this. The journal article is entitled " Synthetic LXR ligand inhibits the development of atherosclerosis in mice (http://www.pnas.org/cgi/content/abstract/99/11/7604). Is there anyone on the histonet who performs en face analysis and can help? Thank you, Jennifer MacDonald From liz <@t> premierlab.com Thu Jan 18 17:53:34 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 18 17:44:40 2007 Subject: [Histonet] Sudan IV staining In-Reply-To: Message-ID: <000b01c73b5b$dd6bf110$0d00a8c0@domain.Premier> Jennifer We routinely perform this on mouse aortas, it quite labor intensive and requires a person that has the patience and good attention to detail to do the job correctly. It all starts from correctly dissecting out the aorta from the animal initially, but I would guess that rat aortas would be easier than a mouse and we routinely perform this on mouse aortas. I can send the person a powerpoint presentation that might help. I do not have time to help this week, but possibly the middle of next week they could contact me and I could forward the information that I have on the technique. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, January 18, 2007 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sudan IV staining A researcher at an area hospital has asked for some help performing Sudan IV staining of rat aortas. She would like to look for atherosclerotic lesions by en face analysis. I am not familiar with this. I have reviewed the paper she is using as a reference and it does not go into detail about the staining protocol for this. The journal article is entitled " Synthetic LXR ligand inhibits the development of atherosclerosis in mice (http://www.pnas.org/cgi/content/abstract/99/11/7604). Is there anyone on the histonet who performs en face analysis and can help? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jfish <@t> gladstone.ucsf.edu Thu Jan 18 17:49:37 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Thu Jan 18 17:50:28 2007 Subject: [Histonet] Sudan IV staining In-Reply-To: Message-ID: <000601c73b5b$504ec230$8903010a@JFISH> Jennifer, I have not actually done the stain, I dissect and pin out the aortas (100's of them!) and the investigators do the staining and imaging. The paper you referenced was actually edited by our institute President. The protocol we use is: -Place pinned out aortas in 70% EtOH for 5 minutes. -Stain in 0.5% Sudan IV in 1:1 acetone:absolute EtOH. -Differentiate in 80% EtOH for 3 minutes. -Rinse in tap water for 1-2 minutes. -Store in 3% paraformaldehyde in PBS at 4C until imaging can be done. I also googled Sudan IV stain and found a very similar protocol right away at the whitewolf.newcastle.edu.au website, and I'm sure there are others out there. Good luck, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, January 18, 2007 3:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sudan IV staining A researcher at an area hospital has asked for some help performing Sudan IV staining of rat aortas. She would like to look for atherosclerotic lesions by en face analysis. I am not familiar with this. I have reviewed the paper she is using as a reference and it does not go into detail about the staining protocol for this. The journal article is entitled " Synthetic LXR ligand inhibits the development of atherosclerosis in mice (http://www.pnas.org/cgi/content/abstract/99/11/7604). Is there anyone on the histonet who performs en face analysis and can help? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Jan 18 19:59:04 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Jan 18 20:00:06 2007 Subject: [Histonet] Re: Blue Nevus Disease-Help? Message-ID: Scheherazade Humphrey asks about possible immunohistochemical staining of a blue nevus. Blue nevus - an uncommon but not rare benign variant of pigmented nevus - should mark for S100, but it's nearly always an H & E diagnosis. The usual blue nevus isn't a disease, just an isolated skin lesion. Bundles of heavily pigmented spindly melanocytes are present in the dermis but there are usually no nevus cell nests in the basal layer of the epidermis, so the pigment looks bluish when you look at the nevus "on the hoof". Scheherazade - what a name - since my wife's a storyteller - I hear Scheherazade's narrator theme, first stated (I think) by the violin - in Nikolai Rimsky-Korsakoff's orchestral suite of that name - one of my parents' favorite classical pieces, on those fragile old 78 rpm shellac records back when I was a kid in the 1940's - Bob Richmond Samurai Pathologist Knoxville TN From koellinr <@t> amgen.com Thu Jan 18 21:36:08 2007 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Thu Jan 18 21:37:06 2007 Subject: [Histonet] unsubscribe Message-ID: <16834C6DFFA6004C88DE4507FB8AE54405C93B92@wa-mb4-sea.amgen.com> From ree3 <@t> leicester.ac.uk Fri Jan 19 03:27:13 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Jan 19 03:27:28 2007 Subject: [Histonet] project on microwave In-Reply-To: <45AFDE4E.3000906@ebsciences.com> References: <20070118191322.74944.qmail@web37414.mail.mud.yahoo.com> <45AFDE4E.3000906@ebsciences.com> Message-ID: Well a vendor would say that!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: 18 January 2007 20:54 To: rosadel holgado Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] project on microwave Hello: A microwave vendor weighs in: EBS' position is that domestic microwaves not be used for lab work, but then, we're in the position of manufacturing lab microwaves. :-) That said, in this case I can understand the temptation to do so, given the academic context and undoubtedly inherent budget constraints. I would be careful, however: of all the operations lab microwaves can be used for, fixation and tissue processing are the most demanding and require the tightest temperature control. Besides the issue of a temperature probe (most domestic microwaves don't have one) there is the issue of magnetron cycle time; domestic microwaves' cycle times are typically too long to allow the temperature control required for small, delicate histological samples. The chances of cooking your tissue are great. I'd explore other options: local locations with laboratory microwaves who might let you into their lab. Especially for fixation and processing, a true laboratory microwave is the surest path to success. Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you. rosadel holgado wrote: > Hi! > > I am a newbie here and I wonder if it's appropriate to be asking questions here about my project. I am planning to do a project for my MSc soon and I was thinking of doing something about microwave fixation and microwave assisted processing. Does anyone know of a protocol I can cite for using a domestic microwave for the fixation? > > Thanks! > > Rosadel Salita > Histology > Surrey, England > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonyprasad <@t> hotmail.com Fri Jan 19 05:44:41 2007 From: sonyprasad <@t> hotmail.com (sony prasad) Date: Fri Jan 19 05:44:56 2007 Subject: [Histonet] Markers of oxidative damage Message-ID: Hi all, I am trying to get information on markers of oxidative damage (8-OHdG, 4-HNE or any others that might work). I have some formalin fixed paraffin embedded mouse kidneys and I need to assess the oxidative damage to the tissue via immunohistochemistry. If anyone has used such markers and has any protocols or suggestions I would highly appreciate it. Many thanks, Sony Prasad. Research Student, University of Sheffield. _________________________________________________________________ Over 200000 Jobs @ naukri.com ! Choose The Best One http://naukri.com/tieups/tieups.php?othersrcp=4358 From Melissa.Zummak <@t> ssfhs.org Fri Jan 19 07:05:23 2007 From: Melissa.Zummak <@t> ssfhs.org (Zummak Melissa) Date: Fri Jan 19 07:05:39 2007 Subject: [Histonet] Ventana IHC Staining Message-ID: Leslie, I have experienced that problem also and the explanation that was given to us by Ventana was that we must have had an air bubble in the dispenser and unfortunately there is no way for the instrument to sense that there is an air bubble present or that the instrument did not dispense any reagent onto the slide. We always check the dispenser to see if we can see any air bubbles before we use it but that has not always worked. Melissa Zummak AP Supervisor Alverno Clinical Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chaussey, Leslie Sent: Thursday, January 18, 2007 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana IHC Staining We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Fri Jan 19 07:38:51 2007 From: bhewlett <@t> cogeco.ca (bhewlett@cogeco.ca) Date: Fri Jan 19 07:39:09 2007 Subject: [Histonet] Markers of oxidative damage Message-ID: <45b0c9eb.3e7.fba.24717@cogeco.ca> > Hi Sony, 8-OHdG and 8-OG can be demonstrated by means of their biotin-like reactivity. We have seen this in a number of cases when it appeared as a specific background, in both the patient test and negative reagent control when staining for assorted antigens by IHC. See following reference; Struthers L, Patel R, Clark J, Thomas S. Direct detection of 8-oxodeoxyguanosine and 8-oxoguanine by avidin and its analogues. Analytical Biochemistry 1998; 255: 20-31. Regards, bryan > Hi all, > > I am trying to get information on markers of oxidative damage (8-OHdG, 4-HNE > or any others that might work). I have some formalin fixed paraffin embedded > mouse kidneys and I need to assess the oxidative damage to the tissue via > immunohistochemistry. > If anyone has used such markers and has any protocols or suggestions I would > highly appreciate it. > > Many thanks, > Sony Prasad. > Research Student, > University of Sheffield. > > _________________________________________________________________ > Over 200000 Jobs @ naukri.com ! Choose The Best One > http://naukri.com/tieups/tieups.php?othersrcp=4358 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Fri Jan 19 09:27:42 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Jan 19 09:28:03 2007 Subject: [Histonet] Markers of oxidative damage Message-ID: Have you tried a 3-Nitrotyrosine ab? Indirect marker of protein nitration, as a downstream effect of superoxide and NO producing Peroxynitrite (ONOO-) . Dihydrorhodamine 123 could also be used for ONOO-.. Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From wia2005 <@t> med.cornell.edu Fri Jan 19 10:11:49 2007 From: wia2005 <@t> med.cornell.edu (William Ares) Date: Fri Jan 19 10:12:00 2007 Subject: [Histonet] paraffin sectioning troubleshooting Message-ID: As a newbie to paraffin sectioning on a paraffin microtome, I've been plagued with differential results since starting. My current issue is that as they're cut, my sections are rolling back up on themselves instead of coming off the blade with the appearance of a venetian blind (as they were when the sections were looking good). As they appear brittle, I've tried putting the blocks in a humid environment to try to rehydrate them a little, but to no avail. Anyone have any good troubleshooting tips for me? Thanks in advance. William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu From rjbuesa <@t> yahoo.com Fri Jan 19 10:31:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 19 10:31:45 2007 Subject: [Histonet] paraffin sectioning troubleshooting In-Reply-To: Message-ID: <87199.41104.qm@web61213.mail.yahoo.com> William: "Venetian Blind" effect is usualy caused by: 1-blade holder or object too loose 2-too steep blade angle 3-tissue or paraffin too hard 4-calcifiued areas in the block 5-variations in the speed of sectioning 6-too big block or 7-unevenly har tissue The remedies could be, depending on the cause: 1-tighten all movable parts holding blade holder and object 2-reduce the blade angle 3-use "heavy"duty disposable blades 4-rehydrate and decalcify tissue 5-try to cut with "even" strokes 6-if the block is rectangular in shape, set the longer axis parallel to the blade's edge 7-set the hardest areas to be cut first. If sections rolls over themselves this is usually caused by a too thick setting of the "thickness" knob = abobe 10 ?m. Poor infiltration or a hot block can be also the causes. Set your microtome to section at 5 ?m and cool the block adequately. Hope this will help you! Ren? J. William Ares wrote: As a newbie to paraffin sectioning on a paraffin microtome, I've been plagued with differential results since starting. My current issue is that as they're cut, my sections are rolling back up on themselves instead of coming off the blade with the appearance of a venetian blind (as they were when the sections were looking good). As they appear brittle, I've tried putting the blocks in a humid environment to try to rehydrate them a little, but to no avail. Anyone have any good troubleshooting tips for me? Thanks in advance. William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From mari.ann.mailhiot <@t> leica-microsystems.com Fri Jan 19 10:24:32 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Jan 19 10:51:28 2007 Subject: [Histonet] paraffin sectioning troubleshooting In-Reply-To: Message-ID: William Can you tell me what type of tissue are you cutting? Do you do your own tissue processing or is sent to another lab? What paraffin do you use? What blade do you use, disposable or steel. Sometimes brittleness comes from taking the water out of the tissue. That can be helped by improving your processing schedule. Let me know the answers when you can. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com William Ares To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] paraffin sectioning troubleshooting 01/19/2007 10:11 AM As a newbie to paraffin sectioning on a paraffin microtome, I've been plagued with differential results since starting. My current issue is that as they're cut, my sections are rolling back up on themselves instead of coming off the blade with the appearance of a venetian blind (as they were when the sections were looking good). As they appear brittle, I've tried putting the blocks in a humid environment to try to rehydrate them a little, but to no avail. Anyone have any good troubleshooting tips for me? Thanks in advance. William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From asmith <@t> mail.barry.edu Fri Jan 19 11:43:51 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Jan 19 11:43:57 2007 Subject: [Histonet] paraffin sectioning troubleshooting In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E402D@exchsrv01.barrynet.barry.edu> Frequently one can improve the cutting of a problem block by holding the flat side of an ice cube against the block for 1 minute and then cutting a ribbon of 20 to 30 sections before the block has time to warm up. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Ares Sent: Friday, January 19, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin sectioning troubleshooting As a newbie to paraffin sectioning on a paraffin microtome, I've been plagued with differential results since starting. My current issue is that as they're cut, my sections are rolling back up on themselves instead of coming off the blade with the appearance of a venetian blind (as they were when the sections were looking good). As they appear brittle, I've tried putting the blocks in a humid environment to try to rehydrate them a little, but to no avail. Anyone have any good troubleshooting tips for me? Thanks in advance. William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From kfineout <@t> hotmail.com Fri Jan 19 12:08:53 2007 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Fri Jan 19 12:09:12 2007 Subject: [Histonet] FNA Billing Message-ID: I'm wondering what CPT codes you can bill when receiving an FNA into your lab and making Thinprep slides for your pathologist to interpret?? Thanks for your input. Kelly _________________________________________________________________ [1]Get FREE Web site and company branded e-mail from Microsoft Office Live References 1. http://g.msn.com/8HMAENUS/2734??PS=47575 From gcallis <@t> montana.edu Fri Jan 19 12:20:48 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jan 19 12:20:44 2007 Subject: [Histonet] Spam not related to Histonet but is vendor related Message-ID: <6.0.0.22.1.20070119111136.01b3c5d8@gemini.msu.montana.edu> Dear Histonetters, Have you been hit with spam messaging about the new Ventana Pathway, whatever? I have just been spammed, totally unrelated to Histonet for investing, or whatever in Ventana, blah, blah, blah. Being a busy, but TGIF and hating spam with a passion - this really hacked me off big time! Ventana should know better than to allow whoever, or whatever to spam in this willy nilly junk fashion. I would rather have them contact me directly than see them join in with the other boring, excessive spammers. Hopefully, they are looking in on this email and then change their spammy ways. I would be happy to discuss this with them one on one at any time. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From AGrobe2555 <@t> aol.com Fri Jan 19 12:23:22 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Jan 19 12:23:46 2007 Subject: [Histonet] Paraffin sectioning troubleshooting Message-ID: How thick are your sections? Have you tried adjusting your knife angle? I keep my blocks in the refrigerator the day I am sectioning, and take them out just before sectioning. I allow them to warm for a few minutes, and ribbon off my sections. If I start having problems, I put a frozen artificial ice pack on the block surface for 30-60 seconds, allow to warm for a few seconds, then section again. The brittleness of your sections sounds like a processing issue. You may need to adjust your processing schedule if the tissues are brittle, especially if you are using an automatic processor. If the wax is brittle, try another wax formulation with additional plasticizers that serve to "soften" the wax. From plaurie <@t> benaroyaresearch.org Fri Jan 19 13:20:44 2007 From: plaurie <@t> benaroyaresearch.org (Patrick Laurie) Date: Fri Jan 19 13:21:09 2007 Subject: [Histonet] Double staining of 2 antibodies with 2 different retrieval methods Message-ID: <4f7b0fea590ccd4c8d23b73df6d05f14@localhost.localdomain> Hello Histonet, I am trying to do some double labeling of ubiquitin and HLA-DR LN3 antibody on human spinal cord. My PI wants to be able to see both the ubiquitinization of the motor neurons with patients that had ALS, as well as seeing the proliferation and activation of microglia on the same slide, near the same cell. But, I have found that the ubiquitin I am using, (Chemicon's Monoclonal ubiquitin antibody) requires no antigen retrieval (AR), while the HLA-DR LN3 antibody (monoclonal from MP biomedical) works best with a 30 min citrate retrieval. The citrate retrieval with the Ubiquitin creates too much background staining, blocking reagents are powerless to stop it. Sooo, to make a long story short, what would be the easiest way to get my PI's (not my) desired result? He wants to stay with chemicons monoclonal ubiquitin, Dako's is a polyclonal, but it also doesn't want AR. Does anyone have any suggestions, is this a hopeless cause? Thanks in advance for your advice, Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 From hej01 <@t> health.state.ny.us Fri Jan 19 13:32:42 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Fri Jan 19 13:32:56 2007 Subject: [Histonet] Embedding Station Message-ID: Hi histonetters, Our lab would like to purchase a new embedding station. What would you recommend? Helen Johnson (hej01@health.state.ny.us) From Rcartun <@t> harthosp.org Fri Jan 19 13:36:35 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jan 19 13:36:57 2007 Subject: [Histonet] Date of Service Message-ID: <45B0D7730200007700003D8B@hcnwgwds01.hh.chs> When you receive a consult from another institution, what date of service do you use? The date the consult comes in your door or the date on the paperwork? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From JWEEMS <@t> sjha.org Fri Jan 19 13:39:06 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jan 19 13:39:39 2007 Subject: [Histonet] Date of Service Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D308E@sjhaexc02.sjha.org> the date we receive it -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Friday, January 19, 2007 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Date of Service When you receive a consult from another institution, what date of service do you use? The date the consult comes in your door or the date on the paperwork? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Melissa.Gonzalez <@t> cellgenesys.com Fri Jan 19 13:43:31 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Fri Jan 19 13:43:43 2007 Subject: [Histonet] mouse DC marker on FFPE? Message-ID: Hi all, I was wondering if anyone knows of a good product for dendritic cell detection for mouse on formalin fixed paraffin embedded tissues? So far I've had no luck with CD11c's (on paraffin sections). I was thinking of trying one the human CD1a's (preferably not a mouse monoclonal) & seeing if it cross-reacted. For example, we routinely use a rabbit x human CD3e on mouse FFPE and it works fantastic. Thanks a lot, Melissa From tim.morken <@t> thermofisher.com Fri Jan 19 13:58:02 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Fri Jan 19 13:58:36 2007 Subject: [Histonet] QC/QA IHC position open at ThermoFisher Scientific - Lab Vision, California Message-ID: Here is a postion open at Lab Vision (a ThermoFisher Scientific Company). It is all IHC with some sectioning involved. This is a small group so everyone is involved in a variety of interesting projects besides the QC work. Considering the cost of living in the San Francisco area we will probably hire from the local (california) area. Please send resumes to me at the address or email below. QC Research Associate II Summary of Job: Performs quality control tests on antibodies, detection systems, and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. Major Responsibilities: Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs product IHC stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with Lab Vision/NeoMarkers' Quality Policies and Quality Procedures. Able to work closely with other departments in reaching company goals. Education or Equivalence of Experience: BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. Certification as Histotechnologist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Responsibilities: May oversee work of QC Research Associate I. Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From Jackie.O'Connor <@t> abbott.com Fri Jan 19 15:22:19 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jan 19 15:22:53 2007 Subject: [Histonet] Software needed for Shurmark cassette/slide printer In-Reply-To: Message-ID: A friend of mine (sort of) inherited a Shurmark Cassette and Slide printer - however, he was not able to get the software to run the equipment. Does anyone have a copy of the software he can use to install in a PC? Is that possible? Thanks - Jackie O' From mtarango <@t> nvcancer.org Fri Jan 19 16:27:21 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Jan 19 16:27:45 2007 Subject: [Histonet] Date of Service Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F9926@NVCIEXCH02.NVCI.org> Date it comes to us at our lab. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, January 19, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Date of Service When you receive a consult from another institution, what date of service do you use? The date the consult comes in your door or the date on the paperwork? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From jayant_limaye <@t> yahoo.com Sat Jan 20 10:10:53 2007 From: jayant_limaye <@t> yahoo.com (jayant limaye) Date: Sat Jan 20 10:11:02 2007 Subject: [Histonet] Collaboration Solutions for Clinical Labs and BioPharma Message-ID: <20070120161053.40358.qmail@web51310.mail.yahoo.com> Hello Histonet members, Sending an email to this list as an alumni of UT Southwestern and presently founder of a company called Gencache which specializes in collaboration solutions for Clinical labs and Biopharma. Wish to personally connect with you, presenting you with a proposal for your respective labs. **We also wish to discuss opportunities of setting up a lab in Orange County California, affiliated to your respective labs. We have recently helped develop a highly successful end to end LIMS/LIS solution for an oncology lab in Irvine, California. We have been working with a lot of clinical diagnostics and biotechnology companies in the West coast(California) for the last 2 years. Started this group after having been the Principal Software Engineer R&D at Quest Diagnostics (Nasdaq: DGX). We are presently based both in Irvine, California and India. We are planning on introducing a new Research Process Management System. Please do let us know when you would be interested in our platform. We would like to have your teams as beta customers for our Gencache RPMS (Research Process Management System) which promises to be an exciting collaboration platform for Research. We will customize the RPMS for you and your affiliate organizations. This will include custom Lab/Discovery Informatics as well. You will also be very interested in our business model. We have a very unique Business model and proposition to you (in fact please do look at our clientele of diagnostic labs): Only 1 Virtual Resource will be billed to your individual lab at X dollars per hour (this includes Process Management a key factor in the above process, a Project Manager and a multiplex of off-shore programmers. your lab billed for only 1 virtual resource, no matter number of extra developers working on the project). Our primary revenue comes from consulting for diagnostic labs, some of them prominently in the cancer diagnostics space. Here's an interview, the reason for sharing this interview is to introduce my background, my work for Quest Diagnostics and the Human Genome project amongst others in the past. http://www.socaltech.com/fullstory/0001300.html Our team has grown and so have our product offerings and services. We hope we can share a fruitful and effective working relationship with you in the near future. Look forward to hearing back from you, Do call me at (949)-273-0405. Best Regards, Jayant Limaye Gencache LLC, Irvine, California http://www.gencache.com Collaboration Solutions for BioPharma Phone: (949)-273-0405 From POWELL_SA <@t> Mercer.edu Sat Jan 20 13:36:47 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Sat Jan 20 13:37:25 2007 Subject: [Histonet] Georgia Society for Histotechnology Meeting April 13-15, 2007 Message-ID: <01MC5M72GZ428WWY97@Macon2.Mercer.edu> Hi Friends, I am again posting the program information for our meeting. The program and the registration form can be printed from here. If you want the entire program, I can email you individually with it attached. ****Please note, the deadline for registration at Callaway Garden Mountain Creek Inn is March 1st so please make your reservations early. This is their peak season and rooms go fast, but this rate is exceptional. $119 for single, double, triple or quad. Come on down to Georgia. April 13-15, 2007 Shirley Powell GSH Secretary HOTEL INFORMATION Mountain Creek Inn www.callawaygardens.com 1-800 225-5292 for Reservations Please state you are attending the GSH/ Meeting when making reservations in order to get the discounted rate of $119 for single, double, triple or quad. The deadline for reservations is March 1st to take advantage of this rate. This reduced rate includes lodging, admission to the Gardens, use of the Fitness Center, in room coffee, newspaper, and telephone access fees will be waived. Rates are good three days prior to and three days after the conclusion of the meeting. So plan your vacation early. Bring your family to enjoy the Gardens, golfing, fly fishing, bass fishing, educational tours, tennis, shopping, and the beautiful Sibley Horticulture Center, The Callaway Discovery Center, The Pioneer Log Cabin, The Gardens' bike and walking trails, Mr. Cason's Vegetable Garden, The Day Butterfly Center, and the area scenery and flowers. CALL NOW TO GET THE GOOD RATE Georgia Society for Histotechnology 2007 Program April 13-15, 2007 Callaway Gardens, GA Mountain Creek Inn Friday Evening Program Registration: 5 to 7 Vendor's Reception: Friday Evening from 7 to 9 p.m. Saturday Program 7:00-8:00 am Registration 8:00: IHC From a Pathologist Perspective Dr. Michael Howard 8:45: Basic Immunofluorescence Dr. Ana Maria Abreu-Velez 9:30: Disease We Don't Normally See Dr. Sheriff Zaki - CDC (10:00 - 10:30 am Break) 10:30: Research Histology Pat Grier - CDC 12:00 - 1:00-GSH AWARDS LUNCHEON 1:00: Osteoarthritis Research Veterinary Pathology Vicki Kalscheur - UWM Vet School 1:45: What is Veterinary Histology? Vicki Kalscheur - UWM Vet School 1:30-4:30: Workshop: Basic IHC, Staining Methods, Antigen Retrieval/Enzyme Digestion, Dilutions, Automatic and Manual Staining, Troubleshooting. Dave Reberry and Chris Sheeder - DAKO (3:00 - 3:30 am Break) 4:30-5: Pascal Dave Reberry and Chris Sheeder - DAKO 5:00: GSH General Membership Meeting (GSH Board Meeting to Follow) Sunday Program 8:00-12 Noon: Workshop: A Better Understanding of Automated Immunohistochemical Staining This workshop will review the immunostaining process on an automated instrument, and explore the many factors that can effect staining. Beth L. Roche HTL(ASCP)QIHC - Ventana Medical Systems, Inc. 10:00 - 10:30 am Break REGISTRATION FORM Name_________________________________ Address________________________________ ___________________________________ Email________________________________ Home Phone: Employer___________________________ Address_____________________________ ____________________________________ Work Phone_________________________ Fees: Saturday & Sunday $80______ Student: Half price $40______ Course Instructor's signature required here for Student rate: ____________________________________ Non-refundable Registration Fee $25____ Includes Awards Lunch Saturday TOTAL _____ Program Registration Deadline is April 1, 2007 -- Late Registration fee is $15_____ Please make checks payable to: Georgia Society for Histotechnology Mail to Registrar: Shirley Powell, GSH Secretary MUSM 1550 College Street Macon, GA 31207 From proctoth <@t> ohsu.edu Sat Jan 20 17:52:56 2007 From: proctoth <@t> ohsu.edu (Thomas Proctor) Date: Sat Jan 20 17:53:33 2007 Subject: [Histonet] hydorgen peroxide acitvity Message-ID: Can some one explain why, after de-waxing paraffin sections and into 95% ethanol, most protocols say to treat with hydrogen peroxide in mehtanol? It's the methanol part that I'm not understanding. I would appreciate some input on the reason, as well as any alternative methods. Thanks. Thomas From vonavi <@t> inbox.lv Sat Jan 20 20:02:40 2007 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Sat Jan 20 20:02:50 2007 Subject: [Histonet] Technovit 9100 new fails to polymerize Message-ID: <1169344960.45b2c9c096223@www.inbox.lv> Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Now the main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled 3 ml trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard (we cannot use EXAKT Cutting/Grinding system when our blocks are like a jellyfish). We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where the problem is… May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). May be it is not necessary to use sealed moulds (however, in the manufacturer’s protocol it is written to use sealed moulds). What should I do? May be the polymerization of Technovit 9100 New is only theoretically but not practically possible… Thank you in advance, Andrey From laugh <@t> TheWorld.com Sat Jan 20 21:47:42 2007 From: laugh <@t> TheWorld.com (Steve Fossey) Date: Sat Jan 20 21:47:53 2007 Subject: [Histonet] histology equipment Message-ID: <200701210347.l0L3lg0Q6064834@shell01.TheWorld.com> I'll be quite honest, we sell and service pathology equipment. We're always trying to update or offerings. What I'd love to know is, what are your expriences with the Shurwave microwave tissue processor, and the TBS RCM-7000 coverslipper? If you have h ad good or bad experiences, I'd like to know. thanks, Steve Fossey 514-457-4248 laugh@theworld.com From rjbuesa <@t> yahoo.com Sun Jan 21 08:03:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 21 08:03:48 2007 Subject: [Histonet] hydorgen peroxide acitvity In-Reply-To: Message-ID: <207430.99163.qm@web61216.mail.yahoo.com> Thomas: The methanol is not necessary at all. Hydrogen peroxide after dewaxing and before starting the IHC procedure is used, as I am sure you know, to quench the natural peroxidase, an enzyme present in all cells and used in the respiratory cycle. Since the IHC process used peroxidase-anti-peroxidase steps, the internal peroxidase has to be "oxidized" = render inactive with the hydrogen peroxide but, I repeat, the methanol is not necessary; an aqueous 3% solution of H2O2 during 5 minutes will quench the peroxidase in any FFPE tissue section. Hope this will help you! Ren? J. Thomas Proctor wrote: Can some one explain why, after de-waxing paraffin sections and into 95% ethanol, most protocols say to treat with hydrogen peroxide in mehtanol? It's the methanol part that I'm not understanding. I would appreciate some input on the reason, as well as any alternative methods. Thanks. Thomas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From c.m.vanderloos <@t> amc.uva.nl Mon Jan 22 02:42:37 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Jan 22 02:43:20 2007 Subject: [Histonet] RE: Double staining of 2 antibodies with 2 different Message-ID: <7e38087e2502.7e25027e3808@amc.uva.nl> Patrick, With this type of trouble there are usually no solutions. However, in your case there is perhaps one: You may perform your double staining sequentially starting without antigen retrieval. Incubate with the ubiquitin antibody followed by an appropriate polymer/HRP. Visualize HRP activity with DAB (obligatory!). Then perform HIER with citrate and continue with the HLA-DR antibody etc. ending with AP activity in red (allowing a weak hematoxilin counterstain). In general, a brown-red color combination is not suitable to observe real co-localization by mixed colors. However, you said that you are looking for structures near to each other, so this will be no problem for you. Hope this helps! Good luck, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 19 Jan 2007 11:20:44 -0800 From: "Patrick Laurie" Subject: [Histonet] Double staining of 2 antibodies with 2 different retrieval methods To: "histonet@lists.utsouthwestern.edu" Hello Histonet, I am trying to do some double labeling of ubiquitin and HLA-DR LN3 antibody on human spinal cord. My PI wants to be able to see both the ubiquitinization of the motor neurons with patients that had ALS, as well as seeing the proliferation and activation of microglia on the same slide, near the same cell. But, I have found that the ubiquitin I am using, (Chemicon's Monoclonal ubiquitin antibody) requires no antigen retrieval (AR), while the HLA-DR LN3 antibody (monoclonal from MP biomedical) works best with a 30 min citrate retrieval. The citrate retrieval with the Ubiquitin creates too much background staining, blocking reagent! s are pow From MSHERWOOD <@t> PARTNERS.ORG Mon Jan 22 09:23:29 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Jan 22 09:23:42 2007 Subject: [Histonet] Re: Cryostat Service Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30EC8@PHSXMB1.partners.org> Thanks to all who responded to my inquiry re: service for our MICROM cryostat. I was emailed the name of a local rep who will be coming in to do the sercvice. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. From Sheri.Meilus <@t> va.gov Mon Jan 22 10:50:16 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Mon Jan 22 10:58:46 2007 Subject: [Histonet] RE: Dendritic cells In-Reply-To: <5co3fo$26hrd5@mtasac2.sac.net.va.gov> References: <5co3fo$26hrd5@mtasac2.sac.net.va.gov> Message-ID: Hi Melissa, Have you tried S-100 to stain the dendritic cells? Depending on their lineage, dendritic cells are immunoreactive with S-100 protein. Sheri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, January 20, 2007 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 38, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FNA Billing (Kelly Larson) 2. Spam not related to Histonet but is vendor related (Gayle Callis) 3. Paraffin sectioning troubleshooting (AGrobe2555@aol.com) 4. Double staining of 2 antibodies with 2 different retrieval methods (Patrick Laurie) 5. Embedding Station (Helen E Johnson) 6. Date of Service (Richard Cartun) 7. RE: Date of Service (Weems, Joyce) 8. mouse DC marker on FFPE? (Melissa Gonzalez) 9. QC/QA IHC position open at ThermoFisher Scientific - Lab Vision, California (Morken, Tim) 10. Software needed for Shurmark cassette/slide printer (Jackie M O'Connor) 11. RE: Date of Service (Tarango, Mark) 12. Collaboration Solutions for Clinical Labs and BioPharma (jayant limaye) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jan 2007 13:08:53 -0500 From: "Kelly Larson" Subject: [Histonet] FNA Billing To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" I'm wondering what CPT codes you can bill when receiving an FNA into your lab and making Thinprep slides for your pathologist to interpret?? Thanks for your input. Kelly _________________________________________________________________ [1]Get FREE Web site and company branded e-mail from Microsoft Office Live References 1. http://g.msn.com/8HMAENUS/2734??PS=47575 ------------------------------ Message: 2 Date: Fri, 19 Jan 2007 11:20:48 -0700 From: Gayle Callis Subject: [Histonet] Spam not related to Histonet but is vendor related To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070119111136.01b3c5d8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Dear Histonetters, Have you been hit with spam messaging about the new Ventana Pathway, whatever? I have just been spammed, totally unrelated to Histonet for investing, or whatever in Ventana, blah, blah, blah. Being a busy, but TGIF and hating spam with a passion - this really hacked me off big time! Ventana should know better than to allow whoever, or whatever to spam in this willy nilly junk fashion. I would rather have them contact me directly than see them join in with the other boring, excessive spammers. Hopefully, they are looking in on this email and then change their spammy ways. I would be happy to discuss this with them one on one at any time. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 3 Date: Fri, 19 Jan 2007 13:23:22 EST From: AGrobe2555@aol.com Subject: [Histonet] Paraffin sectioning troubleshooting To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" How thick are your sections? Have you tried adjusting your knife angle? I keep my blocks in the refrigerator the day I am sectioning, and take them out just before sectioning. I allow them to warm for a few minutes, and ribbon off my sections. If I start having problems, I put a frozen artificial ice pack on the block surface for 30-60 seconds, allow to warm for a few seconds, then section again. The brittleness of your sections sounds like a processing issue. You may need to adjust your processing schedule if the tissues are brittle, especially if you are using an automatic processor. If the wax is brittle, try another wax formulation with additional plasticizers that serve to "soften" the wax. ------------------------------ Message: 4 Date: Fri, 19 Jan 2007 11:20:44 -0800 From: "Patrick Laurie" Subject: [Histonet] Double staining of 2 antibodies with 2 different retrieval methods To: "histonet@lists.utsouthwestern.edu" Message-ID: <4f7b0fea590ccd4c8d23b73df6d05f14@localhost.localdomain> Content-Type: text/plain; charset="us-ascii" Hello Histonet, I am trying to do some double labeling of ubiquitin and HLA-DR LN3 antibody on human spinal cord. My PI wants to be able to see both the ubiquitinization of the motor neurons with patients that had ALS, as well as seeing the proliferation and activation of microglia on the same slide, near the same cell. But, I have found that the ubiquitin I am using, (Chemicon's Monoclonal ubiquitin antibody) requires no antigen retrieval (AR), while the HLA-DR LN3 antibody (monoclonal from MP biomedical) works best with a 30 min citrate retrieval. The citrate retrieval with the Ubiquitin creates too much background staining, blocking reagents are powerless to stop it. Sooo, to make a long story short, what would be the easiest way to get my PI's (not my) desired result? He wants to stay with chemicons monoclonal ubiquitin, Dako's is a polyclonal, but it also doesn't want AR. Does anyone have any suggestions, is this a hopeless cause? Thanks in advance for your advice, Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 ------------------------------ Message: 5 Date: Fri, 19 Jan 2007 14:32:42 -0500 From: Helen E Johnson Subject: [Histonet] Embedding Station To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi histonetters, Our lab would like to purchase a new embedding station. What would you recommend? Helen Johnson (hej01@health.state.ny.us) ------------------------------ Message: 6 Date: Fri, 19 Jan 2007 14:36:35 -0500 From: "Richard Cartun" Subject: [Histonet] Date of Service To: Message-ID: <45B0D7730200007700003D8B@hcnwgwds01.hh.chs> Content-Type: text/plain; charset=US-ASCII When you receive a consult from another institution, what date of service do you use? The date the consult comes in your door or the date on the paperwork? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 7 Date: Fri, 19 Jan 2007 14:39:06 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Date of Service To: "Richard Cartun" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D308E@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" the date we receive it -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Friday, January 19, 2007 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Date of Service When you receive a consult from another institution, what date of service do you use? The date the consult comes in your door or the date on the paperwork? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 8 Date: Fri, 19 Jan 2007 11:43:31 -0800 From: "Melissa Gonzalez" Subject: [Histonet] mouse DC marker on FFPE? To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, I was wondering if anyone knows of a good product for dendritic cell detection for mouse on formalin fixed paraffin embedded tissues? So far I've had no luck with CD11c's (on paraffin sections). I was thinking of trying one the human CD1a's (preferably not a mouse monoclonal) & seeing if it cross-reacted. For example, we routinely use a rabbit x human CD3e on mouse FFPE and it works fantastic. Thanks a lot, Melissa ------------------------------ Message: 9 Date: Fri, 19 Jan 2007 14:58:02 -0500 From: "Morken, Tim" Subject: [Histonet] QC/QA IHC position open at ThermoFisher Scientific - Lab Vision, California To: Message-ID: Content-Type: text/plain; charset="us-ascii" Here is a postion open at Lab Vision (a ThermoFisher Scientific Company). It is all IHC with some sectioning involved. This is a small group so everyone is involved in a variety of interesting projects besides the QC work. Considering the cost of living in the San Francisco area we will probably hire from the local (california) area. Please send resumes to me at the address or email below. QC Research Associate II Summary of Job: Performs quality control tests on antibodies, detection systems, and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. Major Responsibilities: Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs product IHC stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with Lab Vision/NeoMarkers' Quality Policies and Quality Procedures. Able to work closely with other departments in reaching company goals. Education or Equivalence of Experience: BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. Certification as Histotechnologist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Responsibilities: May oversee work of QC Research Associate I. Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com ------------------------------ Message: 10 Date: Fri, 19 Jan 2007 15:22:19 -0600 From: "Jackie M O'Connor" Subject: [Histonet] Software needed for Shurmark cassette/slide printer To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Cc: andrew.lisowski@abbott.com Message-ID: Content-Type: text/plain; charset="US-ASCII" A friend of mine (sort of) inherited a Shurmark Cassette and Slide printer - however, he was not able to get the software to run the equipment. Does anyone have a copy of the software he can use to install in a PC? Is that possible? Thanks - Jackie O' ------------------------------ Message: 11 Date: Fri, 19 Jan 2007 14:27:21 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] Date of Service To: "Richard Cartun" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F9926@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii Date it comes to us at our lab. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, January 19, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Date of Service When you receive a consult from another institution, what date of service do you use? The date the consult comes in your door or the date on the paperwork? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== ------------------------------ Message: 12 Date: Sat, 20 Jan 2007 08:10:53 -0800 (PST) From: jayant limaye Subject: [Histonet] Collaboration Solutions for Clinical Labs and BioPharma To: Histonet@lists.utsouthwestern.edu Message-ID: <20070120161053.40358.qmail@web51310.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histonet members, Sending an email to this list as an alumni of UT Southwestern and presently founder of a company called Gencache which specializes in collaboration solutions for Clinical labs and Biopharma. Wish to personally connect with you, presenting you with a proposal for your respective labs. **We also wish to discuss opportunities of setting up a lab in Orange County California, affiliated to your respective labs. We have recently helped develop a highly successful end to end LIMS/LIS solution for an oncology lab in Irvine, California. We have been working with a lot of clinical diagnostics and biotechnology companies in the West coast(California) for the last 2 years. Started this group after having been the Principal Software Engineer R&D at Quest Diagnostics (Nasdaq: DGX). We are presently based both in Irvine, California and India. We are planning on introducing a new Research Process Management System. Please do let us know when you would be interested in our platform. We would like to have your teams as beta customers for our Gencache RPMS (Research Process Management System) which promises to be an exciting collaboration platform for Research. We will customize the RPMS for you and your affiliate organizations. This will include custom Lab/Discovery Informatics as well. You will also be very interested in our business model. We have a very unique Business model and proposition to you (in fact please do look at our clientele of diagnostic labs): Only 1 Virtual Resource will be billed to your individual lab at X dollars per hour (this includes Process Management a key factor in the above process, a Project Manager and a multiplex of off-shore programmers. your lab billed for only 1 virtual resource, no matter number of extra developers working on the project). Our primary revenue comes from consulting for diagnostic labs, some of them prominently in the cancer diagnostics space. Here's an interview, the reason for sharing this interview is to introduce my background, my work for Quest Diagnostics and the Human Genome project amongst others in the past. http://www.socaltech.com/fullstory/0001300.html Our team has grown and so have our product offerings and services. We hope we can share a fruitful and effective working relationship with you in the near future. Look forward to hearing back from you, Do call me at (949)-273-0405. Best Regards, Jayant Limaye Gencache LLC, Irvine, California http://www.gencache.com Collaboration Solutions for BioPharma Phone: (949)-273-0405 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 38, Issue 30 **************************************** From tim.morken <@t> thermofisher.com Mon Jan 22 11:26:22 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Jan 22 11:26:43 2007 Subject: [Histonet] Belinda Cairns? Can you contact me off histonet? Message-ID: Belinda, your email does not seem to work. Could you contact me by phone? Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From JWEEMS <@t> sjha.org Mon Jan 22 12:58:12 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jan 22 12:58:57 2007 Subject: [Histonet] Iron and copper code question Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D30A1@sjhaexc02.sjha.org> What is the difference in iron and copper staining that would cause copper and zinc, to to be listed as 88318 and iron listed in 88313 in CPT codes? Seems iron is a histochemical stain also. Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From BNuern <@t> coj.net Mon Jan 22 13:41:23 2007 From: BNuern <@t> coj.net (Nuernberger, Barb) Date: Mon Jan 22 13:41:32 2007 Subject: [Histonet] osmium disposal Message-ID: <66529604C8644845BB24FF6F623566414F5D64@EVS1.coj.net> Does anyone know how to dispose of osmium tetroxide solution that is old? From LRaff <@t> lab.uropartners.com Mon Jan 22 13:50:15 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Mon Jan 22 13:50:23 2007 Subject: [Histonet] fluorescent microscope bulbs Message-ID: <5DA1CA5D0B98A84985B545A24423B822052C7A@UPLAB01.uplab.local> Any recommended vendors for fluorescent microscope bulbs? Also, since these contain mercury, what disposal methods do you use? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From mthomas <@t> littonlab.com Mon Jan 22 15:14:26 2007 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Mon Jan 22 15:14:45 2007 Subject: [Histonet] fluorescent microscope bulbs Message-ID: <000801c73e6a$4c5346d0$9d35a8c0@LittonPath.local> The Missouri Department of Natural Resources has a list of Fluorescent Bulb Recyclers form all over the country. www.dnr.mo.gov/pubs/pub451.pdf Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. From EWURDAK <@t> CSBSJU.EDU Mon Jan 22 15:52:25 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Mon Jan 22 15:53:05 2007 Subject: [Histonet] osmium disposal In-Reply-To: <66529604C8644845BB24FF6F623566414F5D64@EVS1.coj.net> Message-ID: Mix with twice the volume of corn oil and wait for the oil to turn black. Dispose in accordance with local regulations. See pg 57 in the Electron Microscopy Sciences catalog, , for a more detailed description of the procedure and additional references. Elizabeth Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 1/22/07 1:41 PM, "Nuernberger, Barb" wrote: > Does anyone know how to dispose of osmium tetroxide solution that is > old? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Mon Jan 22 16:43:59 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Jan 22 16:44:32 2007 Subject: [Histonet] proficiency handbook Message-ID: <45B4F7DF020000EE000109A3@smtp-gw.hurleymc.com> Hello, I have been asked to compose a handbook for the Michigan Society of Histotechnology on proficiency. There is alot of talent out there, and I am hoping that my fellow histotechs would be willing to share their knowledge. This handbook would be an aid to laboratories to make improvements/monitor on proficiency..........perhaps in checklist format. If you would be able to share what your hospital has, please attach a copy to me. Coming to Michigan?? I'll make you a pie for your help!!! Thanks in advance, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 From debbiekeith <@t> cox.net Mon Jan 22 17:03:01 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Jan 22 17:03:10 2007 Subject: [Histonet] i bought a tissue processor on EBAY! Message-ID: <5.2.0.9.0.20070122154257.02f3ea40@pop.central.cox.net> hey everyone! I've been wrestling with a problem and it finally occurred to me to bring it here. you were all a great resource during the "cryostat debacle of 2006". yes, that time was so traumatic it earned quotation marks.... ;) i've been trying to set up a lab (CHEAP) and i purchased an older shandon hypercenter tissue processor on (GULP) e-bay. it was C-H-E-A-P. it got damaged by the freight company so i'm having to jump through a million hoops to get reimbursed. the key that is required for programming got bent to the point of breaking off... now it is STUCK in the machine. two of the ribbon-cable serial ports were actually bent inward, the reagent hose was broken and the machine used to power up... now turns on to a screen that says "internal error". i have to have a biomedical person look at it... and determine it is not worth repair and send a letter on company letter-head. i called my biomedical person... and they don't work on shandon... NARF! i paid $770 for the thing. what are the odds that i might be able to fix it for less than the price i paid? anyone out there want parts for THEIR hypercenter? maybe it'd be easier to sell what is salvageable AND help other histnetters out there the MIGHT be looking for a reagent reservoir or something? AND if anyone has a shandon citadel laying around they might wanna get rid of... let me know. :) (i work for the CHEAPEST Dr. on the planet!!!!) i appreciate any input... :) debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.17.4/644 - Release Date: 1/22/2007 From debbiekeith <@t> cox.net Mon Jan 22 17:27:04 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Jan 22 17:27:12 2007 Subject: [Histonet] cryo-embedding and other "cool" stuff. :) Message-ID: <5.2.0.9.0.20070122160403.02ebbe18@pop.central.cox.net> for those that helped me with my cryo-issues AND those that were amused/tickled/glad it wasn't them....I realized there was another installation of the "cryostat debacle of 2006". so many people helped me during the "crisis"... and a couple worth mentioning.... Barry (technician) of BelAir, Don (repair technician) from Leica and Jan Minshew (HTL) with Leica. THANK YOU GUYS!!! :) after replacing the parts i'd gotten from BelAir and doing everything Jan suggested, i felt like i sorta figured it all out. the machine still had some odd behaviors, or pecidillos, if you will. overall, i felt i could work around them. maybe even embrace them the way you would the behaviors of your own teenager. begrudginly.... but you'd still hug it when it was behaving. ;) well, after our newfound compatibility.... the ex-sailor was scheduled to work. what could he do in ONE day?! funny you should ask. :) he tightened the screw on top so HARD... that he stripped the threads COMPLETELY out and you could remove the lever but simply lifting it up. eeeeeek. i called the repair folks and they said it would be WEEKS to get the part in. so, i asked "can't i just heli-coil it?" the repair guy was VERY skeptical. he sounded like he didn't even WANT to know what i was up to. (smart man!) i took it home and my hubby fixed it with parts we had in the garage!! (we have a few british bikes in the garage, so there are metric bits/parts all over!) the cryostat was not only GOOD as new... but BETTER! the original part is aluminum.... and the heli-coil is steel... so it is stronger, harder and FASTER. it is the Steve Austin of cryostats. before the holidays i'd been sorta looking at cryo-embedding systems. the ones out there are great... but they aren't really Mohs-Friendly. know what i mean? so i MADE my own. i can't BELIEVE how awesome it IS!!! i did exhaustive research on metals/heat syncs/thermal conductivity... and created a system that reduced my processing time by 50%. my turn--around-time is about 10 minutes from fresh tissue to slides on the scope. (i hand stain!) is it too good to be true....? nope. every day, when i clean the inside of the cryostat... i lovingly caress the chuck holder... and blow a kiss at the cryo-embedding system. (utilizing universal precautions the whole while! natch!) so, now... my slides are purty, my turn-around-time is shorter and my cryostat is making "THE noise". it IS a happy new year, indeed. :) debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.17.4/644 - Release Date: 1/22/2007 From irinarukina <@t> msn.com Mon Jan 22 17:37:37 2007 From: irinarukina <@t> msn.com (Irina Rukina) Date: Mon Jan 22 17:36:19 2007 Subject: [Histonet] Breast Bx stains Message-ID: Hi Everyone! I'm a histotech student and I need some information for my research project. I'm looking at the breast cancer diagnostics from the histological point. What kind of special stains and IHC stains could be done? How often frozen sections are requested? Any kind of information on the subject would be greatly appreciated! Thanks in advance! Irina Rukina Histology Program Argosy University irinarukina@msn.com From webb3655 <@t> sbcglobal.net Mon Jan 22 17:45:30 2007 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Mon Jan 22 17:45:38 2007 Subject: [Histonet] Texas Society for Histotechnology State Convention Message-ID: <625906.24319.qm@web83105.mail.mud.yahoo.com> Save the Date: Texas Society for Histotechnology Annual Convention April 19-22, 2007 Renaissance Houston Hotel Greenway Plaza Programs will be mailed early February. To be added to the mailing list, please email jwebb01@jpshealth.org We are working toward this being the biggest yet! Hope to see you there! Judy Webb McKinney Vice President Texas Society for Histotechnology From omnivore98 <@t> yahoo.com Mon Jan 22 18:23:30 2007 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Mon Jan 22 18:23:48 2007 Subject: [Histonet] embedding stations Message-ID: <579631.41340.qm@web31306.mail.mud.yahoo.com> We just completed the demo on three embedding stations and Sakura won our cap dollars. They were all very nice with different features to please every taste, and the reps were more than accommodating. We have had allot of Sakura instrumentation and they are work horses that seem to last forever. Our old Sakura embedding unit is 10+ years old with rust and has never once had a problem. Heather Renko, HT(ASCP) --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From Melissa.Zummak <@t> ssfhs.org Mon Jan 22 20:07:45 2007 From: Melissa.Zummak <@t> ssfhs.org (Zummak Melissa) Date: Mon Jan 22 20:07:50 2007 Subject: [Histonet] i bought a tissue processor on EBAY! Message-ID: Debbie, I have a Shandon citadel that I would love to get rid of, if you are interested let me know. Melissa Zummak AP Supervisor Alverno Clinical Lab Hammond, IN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Keith Sent: Monday, January 22, 2007 5:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] i bought a tissue processor on EBAY! hey everyone! I've been wrestling with a problem and it finally occurred to me to bring it here. you were all a great resource during the "cryostat debacle of 2006". yes, that time was so traumatic it earned quotation marks.... ;) i've been trying to set up a lab (CHEAP) and i purchased an older shandon hypercenter tissue processor on (GULP) e-bay. it was C-H-E-A-P. it got damaged by the freight company so i'm having to jump through a million hoops to get reimbursed. the key that is required for programming got bent to the point of breaking off... now it is STUCK in the machine. two of the ribbon-cable serial ports were actually bent inward, the reagent hose was broken and the machine used to power up... now turns on to a screen that says "internal error". i have to have a biomedical person look at it... and determine it is not worth repair and send a letter on company letter-head. i called my biomedical person... and they don't work on shandon... NARF! i paid $770 for the thing. what are the odds that i might be able to fix it for less than the price i paid? anyone out there want parts for THEIR hypercenter? maybe it'd be easier to sell what is salvageable AND help other histnetters out there the MIGHT be looking for a reagent reservoir or something? AND if anyone has a shandon citadel laying around they might wanna get rid of... let me know. :) (i work for the CHEAPEST Dr. on the planet!!!!) i appreciate any input... :) debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.17.4/644 - Release Date: 1/22/2007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From farnhaml <@t> smha.org Tue Jan 23 03:44:35 2007 From: farnhaml <@t> smha.org (Farnham, Lori) Date: Tue Jan 23 03:44:53 2007 Subject: [Histonet] Small Hospital Path lab wants to bring in CT/GC and HPV testing Message-ID: Good Morning. Is there any small hosptial labs out there who have brought in CT/GC and HPV testing from a Thin Prep PreservCyt specimen. We currently send out 150CT/GC specimens and 50 HPV specimens a month to a reference lab. We would like to bring this inhouse. If someone out there has done this, what system do you have? Thanks, Lori From HGOOLSBY <@t> PARTNERS.ORG Tue Jan 23 08:42:02 2007 From: HGOOLSBY <@t> PARTNERS.ORG (Goolsby, Holly ) Date: Tue Jan 23 08:42:28 2007 Subject: [Histonet] MGH Neuropath Message-ID: <5F5719B83FF4BF4890D8FFF2552CE28C0276BCCB@PHSXMB17.partners.org> I'd like to let everyone know that MGH Neuropath moved, but never liquidated! Here are some thoughts on Hydroquinone & Lithium Carbonate: Tim, the MGH protocol says that either Hydroquinone or Lithium Carbonate can be used. I think that came from the original lab handbook by DR's Kubik & Richardson. The Kluver-Barrera method uses Lithium Carbonate as from the 1957 AFIP manual and the Holmes Luxol Fast Blue uses the Hydroquinone & Sodium Sulfite. This was a step taken from Bodian procedure. I started using Lithium Carbonate several years ago when I went to use the Hydroquinone/sodium sulfite solution, I discovered that it had turned brown. I didn't have time to remake the solution, so I used the Lithium Carbonate and it worked beautifully. Maybe Kubik & Richardson had the same emergency, I use a super saturated Lithium Carbonate stock solution. Dilute 1:1 before use and filter and use for differentiating the slides after their incubation in Luxol Fast Blue. Harris hematoxylin for 3 min, acid ETOH, lithium carbonate again and then 1% alcoholic eosin with 0.5% acetic acid for 3 min and then into 100% ETOH x 3, xylene x 3. Gill hematoxylin gives poor results for this stain. One last note: pay attention to the washing steps after acid alcohol & lithium carbonate. If not washed for a few minutes between steps, the slides will fade over time. Holly A. Goolsby, HT(ASCP) Sr. Tech Specialist, Neuropathology Pathology, Blake 350 B Massachusetts General Hospital Fruit Street Boston, MA 02114 hgoolsby@partners.org 617-726-3798 fax: 617-726-6829 THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. From Carlos.Prada-Puentes <@t> tst.nhs.uk Tue Jan 23 08:49:17 2007 From: Carlos.Prada-Puentes <@t> tst.nhs.uk (Carlos Prada-Puentes) Date: Tue Jan 23 08:49:55 2007 Subject: [Histonet] WT-1 and others Message-ID: Hello all I was an old member of Histonet back in 1999 and now I need your expertise again. We are doing ThinPrep for most of fluids and FNAs. Our results with immunocytochemistry in ThinPrep are very "inconsistent" especially with nuclear markers like TTF-1, ER and WT-1. With WT-1 we perform some antigen retrieval but it only works with High pH which spoils morphology. I wonder if anyone knows any standarised protocol for ThinPrep and Immuno which works reasonably for most antibodies so our BMS staff can have an easier life. Thanks in advance to all Dr Carlos Prada-Puentes Taunton & Somerset NHS Trust Musgrove Park TAUNTON TA1 5DA Phone 0044 (0) 1823342730 Fax 0044 (0) 1823344431 From relia1 <@t> earthlink.net Tue Jan 23 09:12:12 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 23 09:12:21 2007 Subject: [Histonet] RELIA's Histology Career Bulletin - from Pam Barker 1-23-07 Message-ID: Hi Histonetters!!! Wherever you want to go; Whatever you want to do, if you are looking for a new opportunity I can help. I offer over 20 years of professional recruiting and a recruiting practice dedicated solely to permanent placement in the Histology profession. Introducing: RELIA?s Career Coaching Program. I offer to you FREE of charge: * Assistance with updating or creating your resume * Tips on interviewing * Encouragement and Assistance during the course of your job search * Responsiveness ? I will respond to your e-mails/phone calls within 24 hours or less. * Troubleshooting ? if your job search is stalled or you can?t get in the company you are interested in. * Personalized Job Search ? customized to your experience, wants and needs. * Complete Confidentiality - Your career and my coaching is a relationship of trust. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation and benefits programs. Here are just a few of my current openings: 1. Histology Supervisor ? Massachusetts 2. Histology Supervisor ? Florida 3. Histo Tech ? Florida ? Multiple positions statewide 4. Histo Tech ? Maryland/DC 5. Histo Tech ? Ohio Here are some of my upcoming opportunities: 1. Histology Supervisor ? Georgia 2. Histo Tech ? South Carolina 3. Histo Tech ? California 4. Histo Tech ? Maine 5. Histo Tech ? Massachusetts 6. Histo Tech ? Arizona If you or any of your friends would like more information on RELIA?S Career Coaching or about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look Hope to hear from you soon . Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From rjbuesa <@t> yahoo.com Tue Jan 23 09:16:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 09:16:43 2007 Subject: [Histonet] Iron and copper code question In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D30A1@sjhaexc02.sjha.org> Message-ID: <435380.2673.qm@web61217.mail.yahoo.com> It is probably because iron is almost a routine stain for bone marrow smears and biopsies, while copper (and zinc) are seldom done and always as histochemical stains for specific cases. Any lab may have several iron stains daily, but copper (for Wilson's disease) is perhaps done once every 1 or 2 months. Frecuency in usage may determine the different code. Just an "educated guess" though! Ren? J. "Weems, Joyce" wrote: What is the difference in iron and copper staining that would cause copper and zinc, to to be listed as 88318 and iron listed in 88313 in CPT codes? Seems iron is a histochemical stain also. Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From contact <@t> excaliburpathology.com Tue Jan 23 09:22:04 2007 From: contact <@t> excaliburpathology.com (P Pierce) Date: Tue Jan 23 09:22:15 2007 Subject: [Histonet] i bought a tissue processor on EBAY! Message-ID: <298603.73769.qm@web50113.mail.yahoo.com> Hi Debbie, give me a call. I can walk you through some of your problems. I have 2 Hypercenters I have practically rewired. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com From JaynesF <@t> pediatrics.ohio-state.edu Tue Jan 23 09:53:49 2007 From: JaynesF <@t> pediatrics.ohio-state.edu (Jaynes, Florinda) Date: Tue Jan 23 09:54:23 2007 Subject: [Histonet] VIP or ESCELSIOR ES Message-ID: We have to make a decision very soon to purchase a Processor Vip or Excelsior ES. We would appreciate any comments from user as to wich one they preffer and wy. Thank you in advance for your feedback, F. Jaynes Senior HT CRI Columbus. Ohio From ttroyer <@t> petersonlab.com Tue Jan 23 10:04:52 2007 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Tue Jan 23 10:05:15 2007 Subject: [Histonet] Microwave Tissue Processors Message-ID: <000801c73f08$3e97c310$6601010a@Peterson.local> Our pathologists are interested in purchasing a microwave tissue processor for smaller biopsies. I was wondering if anyone has any positive and negative feedback. Thanks, Travis From rjbuesa <@t> yahoo.com Tue Jan 23 10:06:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 10:06:19 2007 Subject: [Histonet] VIP or ESCELSIOR ES In-Reply-To: Message-ID: <20070123160606.89131.qmail@web61224.mail.yahoo.com> Besides considering the processing capabilities, that essentially are similar in all tissue processors (TP), consider reliability and in that field Sakura VIPs are at the top of the list. I would always lean towards the VIP. Another thing: do not "fall for" high capacity TP because you will always need the same amount of work pre- and post-tissue processing, regardless of the total capacity or the time required to process the tissue. Besides large capacity TP are soldom used at full capacity. Ren? J. "Jaynes, Florinda" wrote: We have to make a decision very soon to purchase a Processor Vip or Excelsior ES. We would appreciate any comments from user as to wich one they preffer and wy. Thank you in advance for your feedback, F. Jaynes Senior HT CRI Columbus. Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never Miss an Email Stay connected with Yahoo! Mail on your mobile. Get started! From 1dpeterson <@t> meriter.com Tue Jan 23 10:50:41 2007 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Tue Jan 23 10:50:53 2007 Subject: [Histonet] HER2 and the 48 hour rule Message-ID: <328CBAE62F31C642B422970E879DFADC01A80174@pcwex01> Hello Histonetters! Maybe this has been discussed before, and if it has, I apologize in advance, but I need a little input. Now that the CAP has mandated a 6 -48 hour window of fixation time for specimens that may have Her-2 neu performed, what are you doing about weekend specimens? We JUST (after 25+ years) got rid of the need for techs to come in on Saturdays, and would like to be able to continue this trend. However if a breast bx is done at an outside account on a Thursday afternoon, and does not get grossed by our staff until Friday, right now a our processors are set to start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So here are my questions: Do you set up the processor to sit in 70% OH after the formalin fix? Do you let it sit in the paraffin from Saturday until Monday? (Is heat too prolonged?) Or do I have to break the news to staff that if their name is up, and a breast bx comes in, they're coming in Saturday am? We do not have a microwave processor (yet), but soon. Any and all responses will be greatly appreciated! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From twheelock <@t> mclean.harvard.edu Tue Jan 23 11:16:38 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Jan 23 10:55:58 2007 Subject: [Histonet] MGH Neuropath and myelin stain history Message-ID: <45B642F6.6050807@mclean.harvard.edu> Holly! It is good to hear from you again! I am sorry for the misinformation. I had heard, several years ago, that, thanks to managed care, MGH Neuropathology had been absorbed into Surgical Histology. I am glad that it has not been; that would have been a shame, especially being the lab that Dr. E. P. Richardson led. Neuropath is a very specialized field in and of itself, even more so when considering muscle and nerve biopsies. Thanks for setting me--and the Histonet community- straight on that. Thanks also for all the very useful information, starting with a history of the stain at MGH. All I remember was the card that Evi had in the lab by the time I arrived on the scene there. (However, maybe Lithium was mentioned on the card). By the way, I also find that the hydroquinone/sodium sulfite solution occasionally turns brown especially if there is not much left in the bottle or it sits for a long time. I myself just discard the solution, rinse the bottle with distilled water a couple of times, and then make a fresh solution with distilled water. But like you said, sometimes there is just not enough time for that. I am also going to keep the information that you and others have given regarding the Lithium procedure, in case I run into a problem with the hydroquinone. It is great hearing from you. Tim Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From rjbuesa <@t> yahoo.com Tue Jan 23 11:10:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 11:10:52 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80174@pcwex01> Message-ID: <457016.82379.qm@web61217.mail.yahoo.com> Dan: Yours is a mixed question between management and technique issues. Once a favourable working condition has been achieved, breaking it could entail "dire" consequences. Personal schedules could be affected, so I would not advise you to try "the weekend honey-moon" is over approach. The technique approach has to consider if the 48h fixation maximum is to be observed and in that sense I think that leaving the specimens in 70% EthOL is more deleterious than leaving it in the molten wax. Heat has not cummulative effect, but tissues in 70% EthOL have been repported to deteriorate. Confronted with your problem I would set the tissue processors to start the protocol without the weekend delay and leave the tissues in the paraffin. As a "gesture of appreciative gratitude" I would ask somebody to start embedding Monday earlier than usual on those cases. Hope this will help you! Ren? J. "Peterson, Dan" <1dpeterson@meriter.com> wrote: Hello Histonetters! Maybe this has been discussed before, and if it has, I apologize in advance, but I need a little input. Now that the CAP has mandated a 6 -48 hour window of fixation time for specimens that may have Her-2 neu performed, what are you doing about weekend specimens? We JUST (after 25+ years) got rid of the need for techs to come in on Saturdays, and would like to be able to continue this trend. However if a breast bx is done at an outside account on a Thursday afternoon, and does not get grossed by our staff until Friday, right now a our processors are set to start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So here are my questions: Do you set up the processor to sit in 70% OH after the formalin fix? Do you let it sit in the paraffin from Saturday until Monday? (Is heat too prolonged?) Or do I have to break the news to staff that if their name is up, and a breast bx comes in, they're coming in Saturday am? We do not have a microwave processor (yet), but soon. Any and all responses will be greatly appreciated! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From lpaveli1 <@t> hurleymc.com Tue Jan 23 11:11:47 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Jan 23 11:12:14 2007 Subject: [Histonet] VIP or ESCELSIOR ES Message-ID: <45B5FB83020000EE000109F1@smtp-gw.hurleymc.com> We have used both. Started out using the VIP, and when it wore out, naturally assumed that we would again purchase another VIP. Then we demo'd the Excelsior and our histotechs would not let me send it back. I believe that there are two key reasons why. The configuration of the cassette baskets leaves some space in between the cassettes, thus allowing better flow of the solutions. We have had a markedly smaller amount of reprocessed specimens. The other reason why the techs loved it is the dramatically less hands on maintenance. As you know, it is a whole different concept using the "alcohol quality" on the Excelsior. I have been around for 34 years in histo, and frankly, this old dog was a skeptic!!! But no more. We've had it for 3 years, and use it daily. Sometimes old dogs need new toys!! Hope I've helped. Lynette >>> "Jaynes, Florinda" 01/23/07 10:53 AM >>> We have to make a decision very soon to purchase a Processor Vip or Excelsior ES. We would appreciate any comments from user as to wich one they preffer and wy. Thank you in advance for your feedback, F. Jaynes Senior HT CRI Columbus. Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Tue Jan 23 11:16:41 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 23 11:16:53 2007 Subject: [Histonet] VIP or ESCELSIOR ES In-Reply-To: Message-ID: I strongly recommend the VIP 5. They provide excellent tech support and service of their products. Other manufacturers could learn a great deal from this company. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Jaynes, Florinda" >To: >Subject: [Histonet] VIP or ESCELSIOR ES >Date: Tue, 23 Jan 2007 10:53:49 -0500 > > > >We have to make a decision very soon to purchase a Processor Vip or >Excelsior ES. >We would appreciate any comments from user as to wich one they preffer >and wy. > >Thank you in advance for your feedback, > >F. Jaynes >Senior HT >CRI >Columbus. Ohio > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata=081805 From Jason.Wiese <@t> va.gov Tue Jan 23 11:25:54 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Jan 23 11:26:08 2007 Subject: [Histonet] VIP or ESCELSIOR ES In-Reply-To: <20070123160606.89131.qmail@web61224.mail.yahoo.com> References: <20070123160606.89131.qmail@web61224.mail.yahoo.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12776@VHAV20MSGA3.v20.med.va.gov> I disagree. I believe the Excelsior is a superior product for one major reason. Reagent consumption. I have used VIPs every where I have been and they are great. Sakura VIPs may be at the top of the reliability list because they have been around since the dinosaurs. However, do not be afraid of something new just because you are comfortable with something that has been around for years. Since purchasing my Excelsior my reagent usage has been cut almost in half. The Excelsior changes solutions based on specific gravity. You do not change on a schedule. With the VIP you are wasting tons of potentially good reagents unless you are doing some painstaking testing which causes lots of unnecessary exposure for the tech. I remember many times pulling each container from the VIP and pouring from one to another, and then refilling through a ridiculous little hole. This is not an issue with the Excelsior. All reagents are stored internal, and the machine auto rotates when it has used its reagent to max capacity. (Just because that solution looks nasty, doesn't mean it isn't working.) When it rotates you simply pull a one gallon jug from the bottom of the unit and replace with another. Done! The machine pulls the new reagent in and replaces the oldest. Everything else rotates to the next position until it reaches its end point, and the cycle is repeated. You can adjust this to you specific needs with ease. Excelsior = Very little exposure of chemicals to techs and complete usage of chemical products. Not to mention a user friendly touch screen and a great service department and 24/7 tech support. If you want my Excelsior you will have to pry it from my cold dead fingers! I'll say it one more time... Thermo Rocks! Jason E. Wiese, BS, HT(ASCP) Roseburg, OR -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 23, 2007 8:06 AM To: Jaynes, Florinda; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] VIP or ESCELSIOR ES Besides considering the processing capabilities, that essentially are similar in all tissue processors (TP), consider reliability and in that field Sakura VIPs are at the top of the list. I would always lean towards the VIP. Another thing: do not "fall for" high capacity TP because you will always need the same amount of work pre- and post-tissue processing, regardless of the total capacity or the time required to process the tissue. Besides large capacity TP are soldom used at full capacity. Ren? J. "Jaynes, Florinda" wrote: We have to make a decision very soon to purchase a Processor Vip or Excelsior ES. We would appreciate any comments from user as to wich one they preffer and wy. Thank you in advance for your feedback, F. Jaynes Senior HT CRI Columbus. Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never Miss an Email Stay connected with Yahoo! Mail on your mobile. Get started! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Tue Jan 23 11:27:24 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Jan 23 11:27:44 2007 Subject: [Histonet] VIP or ESCELSIOR ES In-Reply-To: <45B5FB83020000EE000109F1@smtp-gw.hurleymc.com> References: <45B5FB83020000EE000109F1@smtp-gw.hurleymc.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12777@VHAV20MSGA3.v20.med.va.gov> Welcome to the new millennium! :) Jason -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Tuesday, January 23, 2007 9:12 AM To: Histonet@lists.utsouthwestern.edu; JaynesF@pediatrics.ohio-state.edu Subject: Re: [Histonet] VIP or ESCELSIOR ES We have used both. Started out using the VIP, and when it wore out, naturally assumed that we would again purchase another VIP. Then we demo'd the Excelsior and our histotechs would not let me send it back. I believe that there are two key reasons why. The configuration of the cassette baskets leaves some space in between the cassettes, thus allowing better flow of the solutions. We have had a markedly smaller amount of reprocessed specimens. The other reason why the techs loved it is the dramatically less hands on maintenance. As you know, it is a whole different concept using the "alcohol quality" on the Excelsior. I have been around for 34 years in histo, and frankly, this old dog was a skeptic!!! But no more. We've had it for 3 years, and use it daily. Sometimes old dogs need new toys!! Hope I've helped. Lynette >>> "Jaynes, Florinda" 01/23/07 10:53 AM >>> We have to make a decision very soon to purchase a Processor Vip or Excelsior ES. We would appreciate any comments from user as to wich one they preffer and wy. Thank you in advance for your feedback, F. Jaynes Senior HT CRI Columbus. Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Tue Jan 23 11:29:49 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Jan 23 11:39:42 2007 Subject: [Histonet] VIP or ESCELSIOR ES In-Reply-To: References: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12778@VHAV20MSGA3.v20.med.va.gov> With a good piece of equipment you shouldn't need a whole lot of tech support... I've said my piece... I'll shut up now. Buy the Excelsior... Jason E. Wiese, BS, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, January 23, 2007 9:17 AM To: JaynesF@pediatrics.ohio-state.edu; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] VIP or ESCELSIOR ES I strongly recommend the VIP 5. They provide excellent tech support and service of their products. Other manufacturers could learn a great deal from this company. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Jaynes, Florinda" >To: >Subject: [Histonet] VIP or ESCELSIOR ES >Date: Tue, 23 Jan 2007 10:53:49 -0500 > > > >We have to make a decision very soon to purchase a Processor Vip or >Excelsior ES. >We would appreciate any comments from user as to wich one they preffer >and wy. > >Thank you in advance for your feedback, > >F. Jaynes >Senior HT >CRI >Columbus. Ohio > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata =081805 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Tue Jan 23 11:52:25 2007 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Tue Jan 23 11:53:23 2007 Subject: [Histonet] Shandon vs Sakura cryostat Message-ID: While on the topic I thought I might as well ask. I will be purchasing a new cryostat this year. I like features on both the Shandon and the Sakura but unsure which to get. I will demo both units before deciding. Any opinions of previous or current user would be appreciated. Thanks. From Lchausse <@t> nmh.org Tue Jan 23 12:03:10 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Tue Jan 23 12:03:40 2007 Subject: [Histonet] Transcriptionist inquiry Message-ID: Are there any histonetters out there who work in a facility with transcriptionists doing transcription from home (or off-site?). If so, would you mind sharing what LIS and/or hardware you're using and some feedback on the pros & cons you've experienced? Thanks in advance for your help. Leslie J Chaussey ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From ploykasek <@t> phenopath.com Tue Jan 23 12:06:08 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jan 23 12:06:37 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80174@pcwex01> Message-ID: Dear Dan, Welcome to the new world of Her2 testing! There will definitely be changes in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all techs to get a copy of the guidelines and read them. That being said, the time in fixation is important for Her2 testing. With heat retrieval I think that too little fixation is worse than >48 hours, but for now we must comply with 6-48 hours. I was taught (a long time ago) that it is best practice for the weekend schedule fixation to reflect the rest of the week, and that it is preferable to leave the tissue longer in xylene. That xylene would have less bad effects than longer time in formalin. A longer time in alcohol would be deleterious to many IHC stains, and not recommended for Her2. Whatever schedule changes you decide to implement, hopefully you can test your new schedule on some tissue before full implementation of a new processing schedule. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello Histonetters! > Maybe this has been discussed before, and if it has, I apologize in > advance, but I need a little input. Now that the CAP has mandated a 6 > -48 hour window of fixation time for specimens that may have Her-2 neu > performed, what are you doing about weekend specimens? We JUST (after > 25+ years) got rid of the need for techs to come in on Saturdays, and > would like to be able to continue this trend. However if a breast bx is > done at an outside account on a Thursday afternoon, and does not get > grossed by our staff until Friday, right now a our processors are set to > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > here are my questions: > Do you set up the processor to sit in 70% OH after the formalin fix? Do > you let it sit in the paraffin from Saturday until Monday? (Is heat too > prolonged?) > Or do I have to break the news to staff that if their name is up, and a > breast bx comes in, they're coming in Saturday am? > We do not have a microwave processor (yet), but soon. > Any and all responses will be greatly appreciated! > > Daniel R Peterson HT(ASCP) > Histopathology Section Head > Meriter Laboratories > (608)-267-6557 > 1dpeterson@meriter.com > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > intended for the sole use of the individual and entity to whom it is > addressed. This message may contain information that is confidential and > is protected by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying or distribution of this > message is strictly prohibited. If you received this message in error, > please immediately notify the sender by reply email and then delete the > message. Thank you. > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From cgfields <@t> lexhealth.org Tue Jan 23 12:11:29 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Tue Jan 23 12:11:55 2007 Subject: [Histonet] Shandon vs Sakura cryostat Message-ID: We bought three Microms and really like them. Low maintanence and easy to use. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Tuesday, January 23, 2007 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon vs Sakura cryostat While on the topic I thought I might as well ask. I will be purchasing a new cryostat this year. I like features on both the Shandon and the Sakura but unsure which to get. I will demo both units before deciding. Any opinions of previous or current user would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From doug <@t> ppspath.com Tue Jan 23 12:15:59 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Jan 23 12:13:38 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80174@pcwex01> Message-ID: <1042547480-213493337@pathology.swmed.edu> Dan, If you dissect the HER2 guideline it will state that "Fixation times for needle biopsies have not been addressed". Will they be addressed? Why aren't they addressed? Why put out this half completed guideline? Also it states "Breast specimens, after appropriate gross inspection and designation of margins, should be promptly sliced at 5- to 10-mm intervals and fixed in formalin (unsliced samples should not be fixed)". So what I take from this is that the breast specimens should be received fresh and the cassettes should be placed in formalin. The six hour minimum will start at this time. As for the "unsliced samples should not be fixed", well that is another question that will need to be addressed by CAP before this guideline is due to be in place on 31 Dec 2007. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Tuesday, January 23, 2007 11:51 AM To: histonet@pathology.swmed.edu Subject: [Histonet] HER2 and the 48 hour rule Hello Histonetters! Maybe this has been discussed before, and if it has, I apologize in advance, but I need a little input. Now that the CAP has mandated a 6 -48 hour window of fixation time for specimens that may have Her-2 neu performed, what are you doing about weekend specimens? We JUST (after 25+ years) got rid of the need for techs to come in on Saturdays, and would like to be able to continue this trend. However if a breast bx is done at an outside account on a Thursday afternoon, and does not get grossed by our staff until Friday, right now a our processors are set to start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So here are my questions: Do you set up the processor to sit in 70% OH after the formalin fix? Do you let it sit in the paraffin from Saturday until Monday? (Is heat too prolonged?) Or do I have to break the news to staff that if their name is up, and a breast bx comes in, they're coming in Saturday am? We do not have a microwave processor (yet), but soon. Any and all responses will be greatly appreciated! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Jan 23 12:14:11 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jan 23 12:14:44 2007 Subject: [Histonet] In-situ incubator Message-ID: Hi all. We are looking for a possible alternative to our current in-situ incubation chamber. Currently, we use a micro-probe. While we are quite happy with their performance, acquiring more has become problematic. We placed an order in Sept. 2006 that still has not been filled. I am curious what other labs are using for in-situ incubation chambers. Thank you for the information. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Jackie.O'Connor <@t> abbott.com Tue Jan 23 12:26:13 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jan 23 12:27:01 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: Message-ID: Are you guys saying that after 48 hours in formalin, it is NOT wise to leave samples in 70% etoh? Why? JO'C Patti Loykasek Sent by: histonet-bounces@lists.utsouthwestern.edu 01/23/2007 12:06 PM To "Peterson, Dan" <1dpeterson@meriter.com>, histonet cc Subject Re: [Histonet] HER2 and the 48 hour rule Dear Dan, Welcome to the new world of Her2 testing! There will definitely be changes in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all techs to get a copy of the guidelines and read them. That being said, the time in fixation is important for Her2 testing. With heat retrieval I think that too little fixation is worse than >48 hours, but for now we must comply with 6-48 hours. I was taught (a long time ago) that it is best practice for the weekend schedule fixation to reflect the rest of the week, and that it is preferable to leave the tissue longer in xylene. That xylene would have less bad effects than longer time in formalin. A longer time in alcohol would be deleterious to many IHC stains, and not recommended for Her2. Whatever schedule changes you decide to implement, hopefully you can test your new schedule on some tissue before full implementation of a new processing schedule. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello Histonetters! > Maybe this has been discussed before, and if it has, I apologize in > advance, but I need a little input. Now that the CAP has mandated a 6 > -48 hour window of fixation time for specimens that may have Her-2 neu > performed, what are you doing about weekend specimens? We JUST (after > 25+ years) got rid of the need for techs to come in on Saturdays, and > would like to be able to continue this trend. However if a breast bx is > done at an outside account on a Thursday afternoon, and does not get > grossed by our staff until Friday, right now a our processors are set to > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > here are my questions: > Do you set up the processor to sit in 70% OH after the formalin fix? Do > you let it sit in the paraffin from Saturday until Monday? (Is heat too > prolonged?) > Or do I have to break the news to staff that if their name is up, and a > breast bx comes in, they're coming in Saturday am? > We do not have a microwave processor (yet), but soon. > Any and all responses will be greatly appreciated! > > Daniel R Peterson HT(ASCP) > Histopathology Section Head > Meriter Laboratories > (608)-267-6557 > 1dpeterson@meriter.com > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > intended for the sole use of the individual and entity to whom it is > addressed. This message may contain information that is confidential and > is protected by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying or distribution of this > message is strictly prohibited. If you received this message in error, > please immediately notify the sender by reply email and then delete the > message. Thank you. > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 23 12:34:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 12:34:32 2007 Subject: [Histonet] Shandon vs Sakura cryostat In-Reply-To: Message-ID: <550122.17112.qm@web61218.mail.yahoo.com> Rebecca: Try to get a Leica demo as well. For me they are the best. Ren? J. Rebecca Barnhart wrote: While on the topic I thought I might as well ask. I will be purchasing a new cryostat this year. I like features on both the Shandon and the Sakura but unsure which to get. I will demo both units before deciding. Any opinions of previous or current user would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Tue Jan 23 12:39:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 12:39:27 2007 Subject: [Histonet] In-situ incubator In-Reply-To: Message-ID: <283701.80336.qm@web61211.mail.yahoo.com> Patti: I used a HYBrite from Vysis. Ren? J. Patti Loykasek wrote: Hi all. We are looking for a possible alternative to our current in-situ incubation chamber. Currently, we use a micro-probe. While we are quite happy with their performance, acquiring more has become problematic. We placed an order in Sept. 2006 that still has not been filled. I am curious what other labs are using for in-situ incubation chambers. Thank you for the information. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From doug <@t> ppspath.com Tue Jan 23 12:47:06 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Jan 23 12:44:46 2007 Subject: [Histonet] Shandon vs Sakura cryostat In-Reply-To: <550122.17112.qm@web61218.mail.yahoo.com> Message-ID: I second that. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 23, 2007 1:34 PM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Shandon vs Sakura cryostat Rebecca: Try to get a Leica demo as well. For me they are the best. Ren? J. Rebecca Barnhart wrote: While on the topic I thought I might as well ask. I will be purchasing a new cryostat this year. I like features on both the Shandon and the Sakura but unsure which to get. I will demo both units before deciding. Any opinions of previous or current user would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Gonzalez <@t> cellgenesys.com Tue Jan 23 12:48:41 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Jan 23 12:48:51 2007 Subject: [Histonet] RE: fluorescent microscope bulbs Message-ID: Lester, I use Ushio or Osram. Available for 200 hours of life or 300. You should be able to order them from your local microscope rep. Or google them and you will see suppliers. Melissa ------------------------------ Message: 3 Date: Mon, 22 Jan 2007 13:50:15 -0600 From: "Lester Raff" Subject: [Histonet] fluorescent microscope bulbs To: Message-ID: <5DA1CA5D0B98A84985B545A24423B822052C7A@UPLAB01.uplab.local> Content-Type: text/plain; charset="us-ascii" Any recommended vendors for fluorescent microscope bulbs? Also, since these contain mercury, what disposal methods do you use? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 ------------------------------ Message: 4 Date: Mon, 22 Jan 2007 15:14:26 -0600 From: "Marla Thomas" Subject: [Histonet] fluorescent microscope bulbs To: Message-ID: <000801c73e6a$4c5346d0$9d35a8c0@LittonPath.local> Content-Type: text/plain; charset="US-ASCII" The Missouri Department of Natural Resources has a list of Fluorescent Bulb Recyclers form all over the country. www.dnr.mo.gov/pubs/pub451.pdf Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 ******************** From gentras <@t> vetmed.auburn.edu Tue Jan 23 13:20:19 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Tue Jan 23 13:20:36 2007 Subject: [Histonet] mouse/rat brain pituitary Message-ID: <45B65FF3.500@vetmed.auburn.edu> Hello, does anyone have info on an atlas/manual in which the pituitary of either mouse or rat brain is distinctively displayed? We have a research collaborator who is specifically interested in studying mouse pituitary. But, we have not been able to find an atlas which shows it's exact location in mouse brain. And it is obviously not distinguishable upon gross exam. Your prompt replies will be much appreciated. Atoska :-) -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From tbraud <@t> holyredeemer.com Tue Jan 23 13:31:17 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 23 13:33:53 2007 Subject: [Histonet] Shandon vs Sakura cryostat In-Reply-To: Message-ID: I third that Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Douglas D Deltour Sent: Tuesday, January 23, 2007 1:47 PM To: 'Rene J Buesa'; 'Rebecca Barnhart'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shandon vs Sakura cryostat I second that. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 23, 2007 1:34 PM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Shandon vs Sakura cryostat Rebecca: Try to get a Leica demo as well. For me they are the best. Ren? J. Rebecca Barnhart wrote: While on the topic I thought I might as well ask. I will be purchasing a new cryostat this year. I like features on both the Shandon and the Sakura but unsure which to get. I will demo both units before deciding. Any opinions of previous or current user would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From sheila_adey <@t> hotmail.com Tue Jan 23 13:36:03 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 23 13:36:22 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: Message-ID: This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7207d94d45&mkt=en-ca From sheila_adey <@t> hotmail.com Tue Jan 23 13:42:15 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 23 13:42:27 2007 Subject: [Histonet] VIP or ESCELSIOR ES In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12778@VHAV20MSGA3.v20.med.va.gov> Message-ID: I wish we never had any questions about operating the equipment but we were a group fresh out of school and appreciated the Technical expertise that Sakura offered us. I was not suggesting the equipment was not excellent. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Wiese, Jason VHAROS" >To: "sheila adey" >,, >Subject: RE: [Histonet] VIP or ESCELSIOR ES >Date: Tue, 23 Jan 2007 09:29:49 -0800 > >With a good piece of equipment you shouldn't need a whole lot of tech >support... > >I've said my piece... I'll shut up now. Buy the Excelsior... > >Jason E. Wiese, BS, HT(ASCP) > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila >adey >Sent: Tuesday, January 23, 2007 9:17 AM >To: JaynesF@pediatrics.ohio-state.edu; Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] VIP or ESCELSIOR ES > >I strongly recommend the VIP 5. They provide excellent tech support and >service of their products. Other manufacturers could learn a great deal >from >this company. > > > >Sheila Adey HT MLT >Port Huron Hospital >Michigan > > > > > > >From: "Jaynes, Florinda" > >To: > >Subject: [Histonet] VIP or ESCELSIOR ES > >Date: Tue, 23 Jan 2007 10:53:49 -0500 > > > > > > > >We have to make a decision very soon to purchase a Processor Vip or > >Excelsior ES. > >We would appreciate any comments from user as to wich one they preffer > >and wy. > > > >Thank you in advance for your feedback, > > > >F. Jaynes > >Senior HT > >CRI > >Columbus. Ohio > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________________________________________ >Buy what you want when you want it on Sympatico / MSN Shopping >http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata >=081805 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata=081805 From rjbuesa <@t> yahoo.com Tue Jan 23 13:43:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 13:43:35 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: Message-ID: <20070123194324.915.qmail@web61211.mail.yahoo.com> Sheila: I would never leave tissues in xylene, it makes them very brittle and hard to section. This is one of the reasons why I stopped using xylene altogether in 1998 and substituted it with mineral (where you can leave the tissues for as long as you want). Ren? J. sheila adey wrote: This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From rjbuesa <@t> yahoo.com Tue Jan 23 13:48:29 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 13:48:36 2007 Subject: Fwd: Re: [Histonet] HER2 and the 48 hour rule Message-ID: <638986.20734.qm@web61220.mail.yahoo.com> I meant MINERAL OIL. Ren? J. Rene J Buesa wrote: Date: Tue, 23 Jan 2007 11:43:24 -0800 (PST) From: Rene J Buesa To: sheila adey , ploykasek@phenopath.com, 1dpeterson@meriter.com, histonet@pathology.swmed.edu CC: Subject: Re: [Histonet] HER2 and the 48 hour rule Sheila: I would never leave tissues in xylene, it makes them very brittle and hard to section. This is one of the reasons why I stopped using xylene altogether in 1998 and substituted it with mineral (where you can leave the tissues for as long as you want). Ren? J. sheila adey wrote: This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From Melissa.Zummak <@t> ssfhs.org Tue Jan 23 13:49:47 2007 From: Melissa.Zummak <@t> ssfhs.org (Zummak Melissa) Date: Tue Jan 23 13:49:55 2007 Subject: [Histonet] Microwave Tissue Processors Message-ID: I am in the same boat, we are looking at purchasing new processor(s) very soon to go in the direction of rapid processing and would also like to hear any comments, positive and\or negative about anyone's experience with any model of processor. Anyone had any experience with the Peloris system? Melissa Zummak Alverno Clincal Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, January 23, 2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Tissue Processors Our pathologists are interested in purchasing a microwave tissue processor for smaller biopsies. I was wondering if anyone has any positive and negative feedback. Thanks, Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Jan 23 14:04:27 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Jan 23 14:01:58 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: Message-ID: <1042540977-213884464@pathology.swmed.edu> You can delay start it just as long as the tissue is not in formalin (from gross until it leaves the last formalin on the processor) longer than 48 hours. Let's say the breast hits formalin at 12PM on a Friday. It has to be out of formalin by 12PM on Sunday. Even with the longest processing schedule it will most likely be ready around 12AM Monday morning. Most likely you will not use the maximum 48 hours due to this. Someone will have to come in on the weekend regardless the time. Who has HER2 watch this weekend?? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, January 23, 2007 2:36 PM To: ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] HER2 and the 48 hour rule This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7 207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 23 14:02:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 14:02:39 2007 Subject: [Histonet] Microwave Tissue Processors In-Reply-To: Message-ID: <724843.74525.qm@web61214.mail.yahoo.com> Melissa: Just an aclaration: Peloris is a tissue processor (TP) capable of rapid processing, but it is NOT a microwave TP. According with VisionBioSystems, Peloris can process 1.5 mm biopsies in 50 minutes, 3mm biopsies in 80 minutes and 6mm biopsies in 3 hours. The speed is the result of a proprietary rapid heating capability and high vacuum to accerate infiltration. This particular type of TP or any other for that matter, including microwave TP, do NOT affect the many histology tasks required during the pre-TP and post-TP steps, that remain independent of the processing rate.Tissue processors and how rapid they operate, affect just the time dedicated to TP during the whole workflow, that is independent of all other tasks. Ren? J. Zummak Melissa wrote: I am in the same boat, we are looking at purchasing new processor(s) very soon to go in the direction of rapid processing and would also like to hear any comments, positive and\or negative about anyone's experience with any model of processor. Anyone had any experience with the Peloris system? Melissa Zummak Alverno Clincal Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, January 23, 2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Tissue Processors Our pathologists are interested in purchasing a microwave tissue processor for smaller biopsies. I was wondering if anyone has any positive and negative feedback. Thanks, Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From pkarlisch <@t> psu.edu Tue Jan 23 14:26:54 2007 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Tue Jan 23 14:27:28 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: References: Message-ID: <45B6293E0200008C00036BA8@GWIA02.HERSHEYMED.NET> We do a delay start and leave the specimens in formalin. We do not leave in 70%, xylene or paraffin for any length of time. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "sheila adey" 1/23/2007 2:36 PM >>> This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Jan 23 14:32:37 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Jan 23 14:30:38 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: Message-ID: <1042539258-213987869@pathology.swmed.edu> I have just patented (in my mind) the idea of "HER2-Safe". It is a holding liquid that you can load in your processor. It will be pumped into your retort after the 48 hours of formalin fixation. It will sit in this safe liquid until you are ready to begin normal tissue processing. This liquid will not harm your tissue and it is within CAP guidelines for HER2 testing. No need for your techs to come in over the weekend! Can I get a witness! I am opening up bidding for rights to this idea. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, January 23, 2007 2:36 PM To: ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] HER2 and the 48 hour rule This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7 207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Tue Jan 23 14:30:34 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 23 14:30:48 2007 Subject: Fwd: Re: [Histonet] HER2 and the 48 hour rule In-Reply-To: <638986.20734.qm@web61220.mail.yahoo.com> Message-ID: Hi Rene, I remember you mentioning the mineral Oil in the past. Could you elaborate on the quality of the tissues. I would love to get rid of Xylene. Thanks Sheila Adey HT MLT Port Huron Hospital Michigan >From: Rene J Buesa >To: histonet@lists.utsouthwestern.edu >Subject: Fwd: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 11:48:29 -0800 (PST) > >I meant MINERAL OIL. > René J. > >Rene J Buesa wrote: > Date: Tue, 23 Jan 2007 11:43:24 -0800 (PST) >From: Rene J Buesa >To: sheila adey , ploykasek@phenopath.com, >1dpeterson@meriter.com, histonet@pathology.swmed.edu >CC: >Subject: Re: [Histonet] HER2 and the 48 hour rule > >Sheila: >I would never leave tissues in xylene, it makes them very brittle and hard >to section. This is one of the reasons why I stopped using xylene >altogether in 1998 and substituted it with mineral (where you can leave the >tissues for as long as you want). >René J. > >sheila adey wrote: >This prompts a new question. >I'm curious as to whether other labs that don't work weekends leave their >tissues in formalin with a delayed start or do you start the processor >right >away and leave the tissues in Xylene as stated below? > > > >Sheila Adey HT MLT >Port Huron Hospital >Michigan > > > > > > >From: Patti Loykasek > > >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > > > >Subject: Re: [Histonet] HER2 and the 48 hour rule > >Date: Tue, 23 Jan 2007 10:06:08 -0800 > > > >Dear Dan, > >Welcome to the new world of Her2 testing! There will definitely be >changes > >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge >all > >techs to get a copy of the guidelines and read them. > >That being said, the time in fixation is important for Her2 testing. With > >heat retrieval I think that too little fixation is worse than >48 hours, > >but > >for now we must comply with 6-48 hours. I was taught (a long time ago) >that > >it is best practice for the weekend schedule fixation to reflect the rest > >of > >the week, and that it is preferable to leave the tissue longer in xylene. > >That xylene would have less bad effects than longer time in formalin. A > >longer time in alcohol would be deleterious to many IHC stains, and not > >recommended for Her2. > >Whatever schedule changes you decide to implement, hopefully you can test > >your new schedule on some tissue before full implementation of a new > >processing schedule. > > > > > >Patti Loykasek BS, HTL, QIHC > >PhenoPath Laboratories > >Seattle, WA > > > > > > > > > > > > > > > Hello Histonetters! > > > Maybe this has been discussed before, and if it has, I apologize in > > > advance, but I need a little input. Now that the CAP has mandated a 6 > > > -48 hour window of fixation time for specimens that may have Her-2 neu > > > performed, what are you doing about weekend specimens? We JUST (after > > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > > would like to be able to continue this trend. However if a breast bx >is > > > done at an outside account on a Thursday afternoon, and does not get > > > grossed by our staff until Friday, right now a our processors are set >to > > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > > here are my questions: > > > Do you set up the processor to sit in 70% OH after the formalin fix? >Do > > > you let it sit in the paraffin from Saturday until Monday? (Is heat >too > > > prolonged?) > > > Or do I have to break the news to staff that if their name is up, and >a > > > breast bx comes in, they're coming in Saturday am? > > > We do not have a microwave processor (yet), but soon. > > > Any and all responses will be greatly appreciated! > > > > > > Daniel R Peterson HT(ASCP) > > > Histopathology Section Head > > > Meriter Laboratories > > > (608)-267-6557 > > > 1dpeterson@meriter.com > > > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > > intended for the sole use of the individual and entity to whom it is > > > addressed. This message may contain information that is confidential >and > > > is protected by law. If you are not the intended recipient, you are > > > hereby notified that any disclosure, copying or distribution of this > > > message is strictly prohibited. If you received this message in error, > > > please immediately notify the sender by reply email and then delete >the > > > message. Thank you. > > > > > > > > > > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >------------------------------------------------------------------------- > >This e-mail message, including any attachments, is for the sole use of > >the intended recipients and may contain privileged information. Any > >unauthorized review, use, disclosure or distribution is prohibited. If > >you are not the intended recipient, please contact the sender by e-mail > >and destroy all copies of the original message, or you may call PhenoPath > >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________________________________________ >http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7207d94d45&mkt=en-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Expecting? Get great news right away with email Auto-Check. >Try the Yahoo! Mail Beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Now that's room service! Choose from over 150,000 hotels >in 45,000 destinations on Yahoo! Travel to find your fit. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA From rjbuesa <@t> yahoo.com Tue Jan 23 14:42:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 23 14:42:49 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: <1042539258-213987869@pathology.swmed.edu> Message-ID: <637089.4652.qm@web61214.mail.yahoo.com> When you start commercial production, please send me the price per gallon! Ren? J. Douglas D Deltour wrote: I have just patented (in my mind) the idea of "HER2-Safe". It is a holding liquid that you can load in your processor. It will be pumped into your retort after the 48 hours of formalin fixation. It will sit in this safe liquid until you are ready to begin normal tissue processing. This liquid will not harm your tissue and it is within CAP guidelines for HER2 testing. No need for your techs to come in over the weekend! Can I get a witness! I am opening up bidding for rights to this idea. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, January 23, 2007 2:36 PM To: ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] HER2 and the 48 hour rule This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7 207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From Heather.D.Renko <@t> osfhealthcare.org Tue Jan 23 15:11:42 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Jan 23 15:11:58 2007 Subject: [Histonet] marking pens Message-ID: <40026EDDE64CDA47AB382C52619ACD3C077752DB@pmc-rfd-mx01.intranet.osfnet.org> I would love to hear about a good histo pen for our cassettes. We are currently having problems with smudging. What are the best histo resistant pens out there that don't smudge. We use Tissue Tek cassettes and use your standard processing reagents. Thank you in advance. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From twheelock <@t> mclean.harvard.edu Tue Jan 23 15:22:17 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Jan 23 15:17:52 2007 Subject: [Histonet] PARAFFIN SECTIONING ADVICE SUMMARY Message-ID: <45B67C89.6000600@mclean.harvard.edu> Hi everyone: Thank you for the advice you gave me concerning the sectioning problems that I have been having recently. I am beginning to systematically use them. In the meantime, I thought that I would summarize them by quoting them in roughly the order that I received them. Hopefully, this synopsis will help other people as well. Thanks again, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 > *PROBLEM* > > I have a serious problem with lines/splits forming in my 5 micron > sections as they come off the microtome. > I just started using Paraplast Xtra Tissue Embedding Medium. > My infiltrating wax is regular Paraplast. > My disposable blades are Surgipath high profile Teflon coated blades. > The microtome is a Microm HM 355S. > I cut human brain tissue exclusively. > > I had been using Surgipath embedding media. > I decided to try the X-tra because I had been having problems with the > sections being thick and thin on just one side of the section. > Also the wax seeming to be thicker (more opaque) on one side of the > block. > The blocks of tissue also were not laying flat in the mold, hence a > lot of trimming after. > There was some problem with lines even with the Surgipath but it was > intermittent., and not nearly as bad as the X-tra. > > I notice that trimming requires more physical effort with the > Paraplast X-tra. > And I have had much more of a problem with lines than with the > Surgipath Embedding Media. > Does it have a higher % of plastic in it. > > I cleaned out the reservoir on the embedding center and played with > the knife angle but to no avail. > I am not sure if the lines have to do with the wax or the blades or both. > > > *REPLIES* > > > 1. I believe that it may be a blade problem. You may want to try > switching blades. Currently I am using the Extremus blade from > Sturkey. It has worked wonderfully for me. I also use the Paraplast > extra wax for embedding (Plus for processing). How do your brains > looks - do they cut well other than the lines in the wax?? Just to be > sure it isn't a processing problem. > > > 2. I would try first changing to another brand of disposable (JUST to > see if there is a difference). > If the problem persists I would return to your usual disposables and > then try adding some bees wax (5%) to your paraffin (JUST to see if > there is a difference). > If the problem remains it could be some dust in the paraffin or in the > molds; have you had problems with the air filters in your lab?I also > cut human brain tissue exclusively. For the past 15 years, I have been > using > > 3. TissuePrep 2 from Fisher. It cuts well and is problem free. You > could check and see if they would send you a sample. > I use DuraEdge blades on an old Leitz microtome. > > 4. I started a new job 6 month ago, and we use the same parrafin and > microtome here as you do. I find the parrafin to be so much better > than the Surgipath. We routinely cut at 3 microns with no problems at > all. This parrafin seems to be a bit more "sticky", but it's worth > it. I wonder if the problem doesn't come from the microtome. Did you > try cutting an empty block? We do this to check / reset the angle on > our microtome, and it's also useful to see if you have "grit" in the > parrafin. 5. One sure-fire way to determine if the lines are the result of your disposable blade is to change blocks and see if the lines are in the same place on the block/within the ribbon. Try a few blocks. If the lines are in the same place, most likely the problem lies with the blade. If the lines show up elsewhere, then it's probably not the blades (it's probably something in the paraffin).You can make up a few "blank blocks" (i.e. no tissue) to make it a little easier to see if the lines stay in the same position or if they change. Sometimes tissue can contain natural components that will cause splits in your ribbon (for example, areas of microcalcification). Using a blank block will eliminate that possibility. > > 6. Stir the paraffin just before embedding to redistribute the plastic > polymers and other additives in paraffin. These tend to settle out at > melting, being heavier - a cheap spatula does the trick. > > 7. Forgot to mention - since you use two different types of paraffin > (one for infiltration, one for embedding), try to observe if the lines > or splits occur where the actual tissue is or whether they occur > around the periphery. If, when you change blocks, you observe that > the lines move...and you consequently determine that it's not your > blade...look to see if the lines/splits are predominantly in areas > around the tissue or in areas within the tissue itself. That may help > narrow down whether it's the embedding paraffin or the infiltration > paraffin, respectively. > If you deduce it to be the paraffin, I would contact the > manufacturer. Your cutting angle should have nothing to do with the > artifact. It's usually a manufacturing issue with the blade, a > manufacturing issue with the paraffin, debris in the wax baths of the > processor or the holding tank of the embedding center, or something > inherent in the tissue itself (I'm not an expert at cutting brain > tissue because I only did so every once in a while, so I'm not sure if > the issue would be something within the tissue itself). > > 8. I don't think the problem is caused entirely by the > disposable blades or the paraffin. Unfortunately, there are a LOT of > other > things to consider. > > Do you turn your paraffin reservoir on the embedding station off and on > daily? If so, it can cause problems if the paraffin does not have > time to > melt completely because different additives have different melting points > and come out of the solid block at different times. If you want to save > energy and turn it off when you are not using it, use as little > paraffin as > possible so it will melt completely in the allotted time. As Gayle > suggested, you should always stir the paraffin right before you use it to > evenly distribute all of the constituents. > > Do you embed using cassettes? I know this sounds odd, but if your > cassettes are not filled up high enough with paraffin the front > portion of > the block (the part with the specimen) can become loose and have just > enough movement to cause problems. If you are making blocks (not using > cassettes) are you clamping down directly on the paraffin. Tightening > down > directly on the paraffin can sometimes cause sectioning variances, > especially if you tighten really hard. It's better to mount the block > on a > wooden, plastic or metal plate and clamp down on that. > > Are you fixing in 10% neutral buffered formalin? If so, are you going > from > fixative into an alcohol that is 70% or less in strength. Higher > concentrations can cause the buffer salts to precipitate in your > solutions > and the tissue. This can make your blocks look like shredded wheat when > you section them. > > Which microtome do you use...how old is it...and how well is it > maintained? > Have you thoroughly inspected the front and back pressure plates on the > blade holder? If there are any irregularities or uneven wear, it will > cause uneven pressure on the blade and produce uneven sectioning like you > described. Does the pressure plate have an adjustment screw (or two) > that > could be improperly aligned? > > How much pressure is being exerted on the blade? People think that if a > little is good then a lot is better, but that's not correct in this > instance. The pressure plate should rest evenly across the blade (the > blade should be centered under the plate if possible) and only a slight > amount of pressure should be applied--just enough to GENTLY hold the > blade > in place and not enough to cause the blade to bow. Too much pressure > will > also cause the problems you described. > > Are all of the locking mechanisms on the microtome, knife holder base, > disposable blade holder, x-y orientation device and specimen holder > locking > correctly? Do the locking levers feel like they are locking > properly--not > rotating past the locking point if you push them and not stopping in > their > rotation before you feel they should? Can you physically move any of the > pieces when they are locked down? > > > > 9. Sometimes it is the temperature of the block when using paraplast. > If the block is too cold it will give thick and thin sections. Try > warming the block up a little and see if that helps. I just set a > couple of blocks on top of the microtome by the time they are cut they > are warmer. Trim the block and then rub your finger over it to warm > it up. > These things have worked for me, I use Paraplast for embedding. > > > 10. Sounds to me that you have a cutting problem or problems, with > thickness variation and lines. > I am assuming that your tissue is relatively uniform in consistency? > > I suggest problem might be one of the following. > 1. Is the knife securely clamped into the holder? If there is any > movement at all on one side this might explain the thickness differences. > 2. Does the holder have wear on one side, either the knife holder > or the sliding mechanism it sits on? 3. Are these blades reliable > as regards having a sharp edge along entire edge? Rene's suggestion > should take care of this. > 4. I do not believe that the wax is the problem unless you have > poorly mixed wax or separation of the components. If this is the > problem then this effect should be seen on different sides of > different blocks in a random manner. > If this wax is too hard then Rene's suggestion of beeswax should help > Would you be kind enough to let us know what size blocks you are cutting? > > > > > Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From mcauliff <@t> umdnj.edu Tue Jan 23 15:27:48 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jan 23 15:27:22 2007 Subject: [Histonet] mouse/rat brain pituitary In-Reply-To: <45B65FF3.500@vetmed.auburn.edu> References: <45B65FF3.500@vetmed.auburn.edu> Message-ID: <45B67DD4.5080607@umdnj.edu> Hi Atoska: The pituitary is not in the brain, it is attached to the ventral surface by a stalk, the infindibulum. Once the brain is removed, the stalk breaks and the pituitary is left in the sphenoid bone. If you cut through the brainstem and carefully lift the brain out of the skull, you will see the infindibulum just posterior to the optic chiasm. Geoff Atoska Gentry wrote: > Hello, does anyone have info on an atlas/manual in which the pituitary > of either mouse or rat brain is distinctively displayed? We have a > research collaborator who is specifically interested in studying > mouse pituitary. But, we have not been able to find an atlas which > shows it's exact location in mouse brain. And it is obviously not > distinguishable upon gross exam. Your prompt replies will be much > appreciated. Atoska :-) > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From hhernandez <@t> pathreflab.com Tue Jan 23 16:30:47 2007 From: hhernandez <@t> pathreflab.com (Hector Hernandez) Date: Tue Jan 23 16:29:39 2007 Subject: [Histonet] Texas Society for Histotechnology State Convention In-Reply-To: <625906.24319.qm@web83105.mail.mud.yahoo.com> Message-ID: You all can visit the Texas Society website at www.txsh.org for more info on the upcoming sate meeting. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of judy webb Sent: Monday, January 22, 2007 5:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texas Society for Histotechnology State Convention Save the Date: Texas Society for Histotechnology Annual Convention April 19-22, 2007 Renaissance Houston Hotel Greenway Plaza Programs will be mailed early February. To be added to the mailing list, please email jwebb01@jpshealth.org We are working toward this being the biggest yet! Hope to see you there! Judy Webb McKinney Vice President Texas Society for Histotechnology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From szero1009 <@t> gmail.com Tue Jan 23 16:44:52 2007 From: szero1009 <@t> gmail.com (David Park) Date: Tue Jan 23 16:45:01 2007 Subject: [Histonet] Mouse Lens cryosectioning Message-ID: <92a26eb40701231444q6142be27pf7f6a4814f8afcce@mail.gmail.com> Dear Histonetters, We have been doing some cryosectioning of murine lenses for immunohistochemistry, and have been having much difficulty in getting good looking sections. Of particular problem is the shattering of the lens, especially towards the center. We have attempted a variety of different methods, including frozen sections that are post-fixed with methanol and acetone and previously fixing the lens in 4% paraformaldehyde for an hour. Are there any other kinds of fixative that you may recommend? I have heard of Davidson's fixative and was wondering whether this would disrupt the actual IHC stainings. We have also tried 4% fixation, and while the lens itself looks good when initially cut, giant cracks begin to form over a short period of time while the slides have been in the cryostat. I have also tried leaving the slides out at room temperature after doing sections to see if the shattering is reduced, but the staining was completely gone, I think possibly because the cytoplasm may have leaked out as a result. Any kind of recommendation, techniques, and so forth are definitely welcome. Thanks. David Park szero1009@gmail.com From liz <@t> premierlab.com Tue Jan 23 17:16:51 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jan 23 17:08:09 2007 Subject: [Histonet] Mouse Lens cryosectioning In-Reply-To: <92a26eb40701231444q6142be27pf7f6a4814f8afcce@mail.gmail.com> Message-ID: <000001c73f44$90a5cee0$0d00a8c0@domain.Premier> David How cold is your cryostat? I would try to section in the warmest cryostat as possible, possibly around -18 or so. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Park Sent: Tuesday, January 23, 2007 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse Lens cryosectioning Dear Histonetters, We have been doing some cryosectioning of murine lenses for immunohistochemistry, and have been having much difficulty in getting good looking sections. Of particular problem is the shattering of the lens, especially towards the center. We have attempted a variety of different methods, including frozen sections that are post-fixed with methanol and acetone and previously fixing the lens in 4% paraformaldehyde for an hour. Are there any other kinds of fixative that you may recommend? I have heard of Davidson's fixative and was wondering whether this would disrupt the actual IHC stainings. We have also tried 4% fixation, and while the lens itself looks good when initially cut, giant cracks begin to form over a short period of time while the slides have been in the cryostat. I have also tried leaving the slides out at room temperature after doing sections to see if the shattering is reduced, but the staining was completely gone, I think possibly because the cytoplasm may have leaked out as a result. Any kind of recommendation, techniques, and so forth are definitely welcome. Thanks. David Park szero1009@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From lpwenk <@t> sbcglobal.net Tue Jan 23 17:31:18 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jan 23 17:31:39 2007 Subject: [Histonet] Iron and copper code question In-Reply-To: <435380.2673.qm@web61217.mail.yahoo.com> Message-ID: <001401c73f46$9684e1a0$cdb52e4b@HPPav2> Could it also be the ease of diagnosis? Iron stains are usually easy - there's usually iron, or there isn't, with a slight variation as to how much iron. And because they are ordered every day, the pathologists have a lot of experience with diagnosing with the Prussian blue. And tissue is usually bone marrow, with an occassional lung (heart failure cells) or spleen thrown in, except in cases of looking for old hemhorrages. But in these cases, low power is good enough - just a quick look is often all that is needed. On the other hand, the amount of copper can be minimal. And if there is cirrhosis, is it caused by the copper (Wilson's), or is any copper found in cirrhotic liver (alcoholism, hepatitis) due to being trapped by the cirrhosis, rather than causing the cirrhosis (Wilson's). If it is Wilson's, the minimal amount of copper can be in any tissue (we used a kidney for years as our control). It takes more time, on higher power, to find these minimal deposits. It seems to me that one criteria for the codes appear to be based on how much time a pathologist has to look at a slide to make a diagnosis. After all, a PAS for fungus is a different code (and billing/reimbursement) than PAS for mucin or glycogen. PAS for Mucin and glycogen are - yes it's there, no it's not. PAS for basement membrane is - yes it's there, but how thick. PAS for fungus is not only is it there or not, but which fungus, and some fungi are harder to find than others (size, number, background). Yet for histotechs - basically, a PAS is a PAS is a PAS. Any variations we do are minor (we leave PAS for basement membrane in the Schiff longer, but we're doing it on 3 um kidney, rather than 5 um for mucin/glycogen/fungus). Hands-on time - it's the same. Peggy Wenk -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 23, 2007 10:17 AM To: Weems, Joyce; Histonet Subject: Re: [Histonet] Iron and copper code question It is probably because iron is almost a routine stain for bone marrow smears and biopsies, while copper (and zinc) are seldom done and always as histochemical stains for specific cases. Any lab may have several iron stains daily, but copper (for Wilson's disease) is perhaps done once every 1 or 2 months. Frecuency in usage may determine the different code. Just an "educated guess" though! Ren? J. "Weems, Joyce" wrote: What is the difference in iron and copper staining that would cause copper and zinc, to to be listed as 88318 and iron listed in 88313 in CPT codes? Seems iron is a histochemical stain also. Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Tue Jan 23 17:33:33 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Tue Jan 23 17:33:45 2007 Subject: [Histonet] marking pens Message-ID: <909945.5574.qm@web58907.mail.re1.yahoo.com> Heather, If I recall correctly this was discussed on Histonet some time ago, and a large number of folks said their favorite for marking cassettes was the good ol' fashioned #2 lead pencil. The markings stayed on the cassettes no matter what treatments and reagents were used. This is a separate issue from slide marking, where things get a little more confusing... Quite a few people had good things to say about Secureline marking pens, but I don't recall if the remarks were re: cassettes or slides or both. The Secureline pens are marketed toward the histology/pathology crowd, and I'm sure they're available from several vendors. For more info, see for example: Best regards, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- I would love to hear about a good histo pen for our cassettes. We are currently having problems with smudging. What are the best histo resistant pens out there that don't smudge. We use Tissue Tek cassettes and use your standard processing reagents. Thank you in advance. Heather Renko ____________________________________________________________________________________ Want to start your own business? Learn how on Yahoo! Small Business. http://smallbusiness.yahoo.com/r-index From Megan.Clarke <@t> hnehealth.nsw.gov.au Tue Jan 23 15:36:15 2007 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Tue Jan 23 17:54:52 2007 Subject: [Histonet] Telomerase antibody Message-ID: <45B71A7F0200002500001DD1@domainatrix.HAHS.HEALTH.NSW.GOV.AU> Hi Histonetters I am a registrar doing some imunohistochemistry stains on formalin fixed paraffin embedded tissue. I have been using a home made antibody to hman anti telomerase, but my results are really not optimal. I have tried every possible retrieval / digestion/ technique.... If any one is using a Telomerase antibody and has any success with staining, would you be so kind as to assist me with information. Thank you Chris Shi Anatomical Pathology HAPS Newcastle Australia From RSRICHMOND <@t> aol.com Tue Jan 23 18:23:07 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Jan 23 18:23:21 2007 Subject: [Histonet] Re: HER2 and the 48 hour rule Message-ID: Here's a truly radical solution: if the pathologist on call comes in on Saturday (or Sunday) morning, let the pathologist embed the breast biopsies! It's about time pathologists learned to embed. Short of that revolutionary approach, I'd want the tissue to fix overnight, then be passed to 70% alcohol, then processed Sunday night. - But it isn't known that this approach works, and it doesn't comply with the rules (which aren't new, actually). But it's got to be preferable to leaving the specimen in xylene (or substitute) or in molten wax. Don't forget the issue of non-formalin fixatives, including glyoxal - they haven't been tried. I'm more concerned about tissue that isn't left in formalin for long enough. If it's not, it fixes in the processor in alcohol. I'm even more concerned about delay in fixation. In many radiology services, stereotactic and wire-localization specimens aren't placed in formalin until after specimen radiography is completed and the specimen is leisurely taken to the pathology lab. I was appalled, but not surprised, by the CAP's failure to address any of these issues. Let's remind ourselves what's at stake here. HER2 status determines whether a patient is treated with trastuzumab (Herceptin), a $50,000 treatment with a potential for damaging the heart. Estrogen receptor determination is also affected by fixation, and expensive and hazardous treatment depends on that also. The lack of concern about this issue by primary care physicians, surgeons, and oncologists once again underlines the "redhaired stepchild" status of anatomic pathology in the laboratory and in the health care system. Remember that when you read the newspapers and magazines and read that faceless "technicians" are responsible for all of these procedures. Bob Richmond Samurai Pathologist Knoxville TN From tam_melville <@t> yahoo.com Tue Jan 23 20:30:10 2007 From: tam_melville <@t> yahoo.com (Tamara Melville) Date: Tue Jan 23 20:30:18 2007 Subject: [Histonet] Stains for Lipids Message-ID: <920080.65932.qm@web38013.mail.mud.yahoo.com> Hello, I would like to stain sections of rat aorta and heart for lipids. I am aware that frozen sections are required however in some paraffin sections stained with H&E I saw some structures resembling fat droplets and would like confirmation by using a stain for lipids. I would like to know if there are any methods to stain lipids in paraffin sections. I would also like to know if tissues fixed and preserved in formal saline can be stained with any of the special lipid stains or are these stains specifically for frozen tissues. Thanks in advance for any assistance. --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From syedab <@t> totalise.co.uk Wed Jan 24 07:05:22 2007 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Wed Jan 24 07:05:37 2007 Subject: [Histonet] embedding without a station? References: <579631.41340.qm@web31306.mail.mud.yahoo.com> Message-ID: <003201c73fb8$4e99c0a0$c6c401a3@LENOVO27D521E3> Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed From Luis.Chiriboga <@t> med.nyu.edu Wed Jan 24 07:23:45 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Wed Jan 24 07:17:45 2007 Subject: [Histonet] FYI: National Institutes of Health Reform Act of 2006 Message-ID: From Elias A. Zerhouni, M.D., Director of the National Institutes of Health (NIH) The National Institutes of Health Reform Act of 2006 and Next Steps I am pleased to announce that the President signed the National Institutes of Health Reform Act of 2006 on January 15, 2007. This follows bipartisan support by the Congress. The Act affirms the importance of the NIH and its vital role in advancing biomedical research to improve the health of the Nation. This is only the third omnibus reauthorization in the NIH?s history, and the first in 14 years. We have initiated an implementation process at NIH to carry out the new legislation. The work is already under way. I have formed an Ad Hoc Working Group of the NIH Steering Committee, to be chaired by the NIH Deputy Director, Dr. Raynard Kington, and comprising Institute and Center (IC) Directors and leadership in legislation, policy, management, communications, extramural and intramural activities, budget, and the Office of the General Counsel, to make recommendations on the implementation of the legislation. The Ad Hoc Working Group will be charged to complete a careful, detailed analysis of the legislation and propose plans for its implementation that will aid the NIH in serving the public and our scientific community more effectively. Key provisions in the Act include items related to (1) the Division of Program Coordination, Planning and Strategic Initiatives; (2) the Common Fund; (3) the Council of Councils; (4) the Scientific Management Review Board; (5) Authorization of Appropriations; (6) Reorganization; and (7) Reporting. 1. The Division of Program Coordination, Planning and Strategic Initiatives (DPCPSI) The DPCPSI, within the Office of the Director, is officially established. The purpose of DPCPSI is to identify and report on research that represents important areas of emerging scientific opportunities, rising public health challenges, or knowledge gaps that deserve special emphasis and would benefit from the conduct or support of additional research that involves collaboration between two or more ICs, or would otherwise benefit from strategic coordination and planning. 2. The Common Fund The Common Fund (CF) will support trans-NIH research. CF amounts will be reserved by the NIH Director, subject to any applicable provisions in appropriations Acts, but the amount reserved as a percentage of the total appropriation in any fiscal year may not be less than the percentage from the preceding fiscal year. The first year that the CF reaches the 5 percent mark, the Director will be required, in consultation with the Council of Councils, to submit recommendations to Congress for changes regarding amounts for the CF. 3. Council of Councils A new Council of Councils will advise on research proposals that would be funded by the Common Fund. It will be composed of 27 members selected from the IC Advisory Councils, individuals nominated by OD offices, and members of the NIH Council of Public Representatives. 4. Scientific Management Review Board (SMRB) At least every 7 years, the SMRB will be required to examine the use of the NIH?s organizational authorities, provide a report on the review, and make recommendations regarding the use of such authorities. If the SMRB recommends an organizational change, the process to effect the change must begin within 100 days of the report, and the change must be fully implemented within 3 years. These requirements do not apply if the NIH Director formally objects to all or part of the recommended organizational change within 90 days, and the objection includes a rationale. 5. Authorization of Appropriations Most expired authorizations of appropriations sections relevant to the NIH will be deleted from the Statute and replaced with one authorization of appropriations for the entire Agency for the following amounts: $30,331,309,000 for FY 2007; $32,831,309,000 for FY 2008; and such sums as may be necessary for FY 2009. 6. Reorganization The legislation requires a public process for certain reorganizations and identifies procedures for any reorganization. 7. Reporting Most reports pertaining to NIH in current law will be deleted and replaced by one biennial report to Congress. Additional reports with respect to collaboration with other DHHS agencies, clinical trials, tissue samples, whistleblowers, and experts and consultants are required. Reports will be required from each institution receiving an NIH award for the training of graduate students for doctoral degrees. ICs will also be required to report to the Director of NIH on the amount of that IC?s budget made available for trans-NIH research. Detailed information about these elements and others is available on the Web at http://www.nih.gov/about/reauthorization/. This affirmation from Congress and the President has come at a critical time, and we want to ensure that we take the best possible advantage of this opportunity. We will be communicating with the community regularly as we make progress in this process. /s/ Elias A. Zerhouni, M.D. NIH Director From jmahoney <@t> alegent.org Wed Jan 24 07:24:22 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Jan 24 07:24:43 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <003201c73fb8$4e99c0a0$c6c401a3@LENOVO27D521E3> References: <579631.41340.qm@web31306.mail.mud.yahoo.com> <003201c73fb8$4e99c0a0$c6c401a3@LENOVO27D521E3> Message-ID: <45B709A60200003C00003052@gwia.alegent.org> Reminds me of a time our embedding centers were out and we had to get creative. it is amazing what you can do with a metal pot of paraffin from the oven, an electric skillet and a tray of ice. It can be done. Jan Omaha, NE >>> "Anila Syed" 01/24/2007 7:05 AM >>> Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Wed Jan 24 08:16:31 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 24 07:55:50 2007 Subject: [Histonet] No embedding center OR tissue processor Message-ID: <45B76A3F.5000905@mclean.harvard.edu> Hi Anila When I first started my lab here in 1986, I had no embedding center or processor. For processing specimens, I used jars that I would have to get up and jiggle every 15 minutes or so, including jars of paraffin stored in the oven. For embedding I would use flat metal "cookie sheets" over ice. What I used as a hot plate to manipulate the specimen in the mold I cannot remember. But a skillet, as Jan suggests (or perhaps an electric stirrer with a heating function) should do it. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From twheelock <@t> mclean.harvard.edu Wed Jan 24 08:24:42 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 24 08:04:09 2007 Subject: [Histonet] MICROTOME BLADES Message-ID: <45B76C2A.6040805@mclean.harvard.edu> Hi Everyone: I am trying other brands of disposable microtome blades. I use Surgipath high profile Teflon coated blades now. What would people recommend? Tim Wheelock Harvard Brain Bank McLean Hospital Belmont , MA 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From jlinda <@t> ces.clemson.edu Wed Jan 24 08:17:08 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Wed Jan 24 08:07:54 2007 Subject: [Histonet] RE: embedding without a station? Message-ID: <5.2.1.1.2.20070124085612.01fd3a18@mailhost.ces.clemson.edu> Anila, You wanted to know what embedding method worked before embedding stations. This is bound to bring out replies from the "old timers" (myself included)! We used an electric paraffin pot with spigot (Lipshaw), a bunsen burner, and an insulated asbestos pad which was normally used nearby a stovetop. You will notice that open flames are discouraged in labs and asbestos...well...that is downright outlawed! The procedure went something like this: Open cassette and remove tissue with forceps, briefly pass tissue through the flame of the bunsen burner( nothing smells quite like singed hair), orient in metal base mold, add paraffin and attach embedding ring. We also had a portable cryoplate that we placed all the embedded samples on. Basically, a little creative engineering should solve your problem. You will need something that will keep your paraffin melted at the optimal temperature and a heated vessel to hold your cassettes in and a portable work surface that can be easily cleaned of paraffin debris. I would be curious to know what you come up with. Thanks, Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From ian.montgomery <@t> bio.gla.ac.uk Wed Jan 24 08:09:25 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Jan 24 08:09:37 2007 Subject: Fw: [Histonet] embedding without a station? Message-ID: <004501c73fc1$41662640$4724d182@ibls.gla.ac.uk> Anila, While an embedding station is handy, all you need is an oven and hotplate to keep the wax molten. Embed as normal, remove from the heat, blow gently across the surface to form a skin of wax then submerge in cold water. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Anila Syed" To: Sent: Wednesday, January 24, 2007 1:05 PM Subject: [Histonet] embedding without a station? > Dear All, > > I have hundreds of carotid plaques to embedd. I have a tissue processor, but > no embedding station. Would anyone attempt to do this without an embedding > station or do you think I should go and try to find the facilities > somewhere? > > What did people do before embedding stations? > > Many thanks for your input and opinions, > > Anila Syed > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jlinda <@t> ces.clemson.edu Wed Jan 24 08:32:35 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Wed Jan 24 08:22:48 2007 Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 34 In-Reply-To: <200701231808.l0NI8Fgt010253@ces.clemson.edu> Message-ID: <5.2.1.1.2.20070124093054.01fa6410@mailhost.ces.clemson.edu> Martine, The Shalaby award...can you please give me more details on this? Or put me in touch with someone who can? Thanks, Linda At 01:08 PM 1/23/2007 -0500, you wrote: >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. RE: VIP or ESCELSIOR ES (Wiese, Jason VHAROS) > 2. RE: VIP or ESCELSIOR ES (Wiese, Jason VHAROS) > 3. Shandon vs Sakura cryostat (Rebecca Barnhart) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Tue, 23 Jan 2007 09:27:24 -0800 >From: "Wiese, Jason VHAROS" >Subject: RE: [Histonet] VIP or ESCELSIOR ES >To: "Lynette Pavelich" , > , > >Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12777@VHAV20MSGA3.v20.med.va.gov> >Content-Type: text/plain; charset="us-ascii" > >Welcome to the new millennium! :) > >Jason > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette >Pavelich >Sent: Tuesday, January 23, 2007 9:12 AM >To: Histonet@lists.utsouthwestern.edu; JaynesF@pediatrics.ohio-state.edu >Subject: Re: [Histonet] VIP or ESCELSIOR ES > >We have used both. Started out using the VIP, and when it wore out, >naturally assumed that we would again purchase another VIP. Then we >demo'd the Excelsior and our histotechs would not let me send it back. >I believe that there are two key reasons why. The configuration of the >cassette baskets leaves some space in between the cassettes, thus >allowing better flow of the solutions. We have had a markedly smaller >amount of reprocessed specimens. The other reason why the techs loved >it is the dramatically less hands on maintenance. As you know, it is a >whole different concept using the "alcohol quality" on the Excelsior. I >have been around for 34 years in histo, and frankly, this old dog was a >skeptic!!! But no more. We've had it for 3 years, and use it daily. >Sometimes old dogs need new toys!! >Hope I've helped. > >Lynette > > >>> "Jaynes, Florinda" 01/23/07 >10:53 AM >>> > > >We have to make a decision very soon to purchase a Processor Vip or >Excelsior ES. >We would appreciate any comments from user as to wich one they preffer >and wy. > >Thank you in advance for your feedback, > >F. Jaynes >Senior HT >CRI >Columbus. Ohio > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 2 >Date: Tue, 23 Jan 2007 09:29:49 -0800 >From: "Wiese, Jason VHAROS" >Subject: RE: [Histonet] VIP or ESCELSIOR ES >To: "sheila adey" , > , > >Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12778@VHAV20MSGA3.v20.med.va.gov> >Content-Type: text/plain; charset="us-ascii" > >With a good piece of equipment you shouldn't need a whole lot of tech >support... > >I've said my piece... I'll shut up now. Buy the Excelsior... > >Jason E. Wiese, BS, HT(ASCP) > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila >adey >Sent: Tuesday, January 23, 2007 9:17 AM >To: JaynesF@pediatrics.ohio-state.edu; Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] VIP or ESCELSIOR ES > >I strongly recommend the VIP 5. They provide excellent tech support and >service of their products. Other manufacturers could learn a great deal >from >this company. > > > >Sheila Adey HT MLT >Port Huron Hospital >Michigan > > > > > > >From: "Jaynes, Florinda" > >To: > >Subject: [Histonet] VIP or ESCELSIOR ES > >Date: Tue, 23 Jan 2007 10:53:49 -0500 > > > > > > > >We have to make a decision very soon to purchase a Processor Vip or > >Excelsior ES. > >We would appreciate any comments from user as to wich one they preffer > >and wy. > > > >Thank you in advance for your feedback, > > > >F. Jaynes > >Senior HT > >CRI > >Columbus. Ohio > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________________________________________ >Buy what you want when you want it on Sympatico / MSN Shopping >http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata >=081805 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 3 >Date: Tue, 23 Jan 2007 12:52:25 -0500 >From: "Rebecca Barnhart" >Subject: [Histonet] Shandon vs Sakura cryostat >To: >Message-ID: >Content-Type: text/plain; charset=US-ASCII > >While on the topic I thought I might as well ask. I will be purchasing >a new cryostat this year. I like features on both the Shandon and the >Sakura but unsure which to get. I will demo both units before deciding. > Any opinions of previous or current user would be appreciated. >Thanks. > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 38, Issue 34 >**************************************** Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From RJLevier <@t> LancasterGeneral.org Wed Jan 24 08:36:34 2007 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Wed Jan 24 08:36:47 2007 Subject: [Histonet] Frozen Section H&E's Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2019E3823@MAIL-LR.lha.org> Hi Folks, I need some help, please. Would someone be willing to share reagents and times of the H&E stain for frozen sections if you are using a xylene substitute? We are having a hard time clearing.... Thanks so much in advance. Becky Rebecca J. LeVier BS HT(ASCP) Histology Supervisor Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From TownsendD <@t> childrensdayton.org Wed Jan 24 08:41:32 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Jan 24 08:42:05 2007 Subject: [Histonet] MICROTOME BLADES Message-ID: We use Stuckey blades and we love them Dolores From asmith <@t> mail.barry.edu Wed Jan 24 08:58:37 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jan 24 08:58:47 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <003201c73fb8$4e99c0a0$c6c401a3@LENOVO27D521E3> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4030@exchsrv01.barrynet.barry.edu> I have never felt that embedding stations are worth the counter space they take up. I just barely melt my Paraplast in a Pyrex beaker on a hotplate and maintain its temperature in a 60 degree oven. I set out the mold on the counter and pour in enough Paraplast to quarter fill it. Since the beaker is hot, I keep a cotton glove on my left hand. I leave my right hand bare to handle the forceps.) I warm my forceps in the flame of an alcohol lamp, and position my tissue on the bottom of the mold, set an embedding ring in place, and fill the mold. I wipe the lip of the beaker with a paper towel (usually one that has already been used for drying my hands). Everything except the oven can be put away in a cupboard, leaving me with counter space for other projects. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed Sent: Wednesday, January 24, 2007 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding without a station? Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From asmith <@t> mail.barry.edu Wed Jan 24 09:06:13 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jan 24 09:06:20 2007 Subject: [Histonet] MICROTOME BLADES In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4031@exchsrv01.barrynet.barry.edu> I think she means "Sturkey" (C.L. Sturkey, 834 Cumberland St., Lebanon, PA). I have been very happy with C.L. Sturkey's "gold" blades ever since I switched to disposables. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, January 24, 2007 9:42 AM To: histonet@lists.utsouthwestern.edu; twheelock@mclean.harvard.edu Subject: Re: [Histonet] MICROTOME BLADES We use Stuckey blades and we love them Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From TownsendD <@t> childrensdayton.org Wed Jan 24 09:21:50 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Jan 24 09:22:11 2007 Subject: [Histonet] MICROTOME BLADES Message-ID: Yes, that's what I meant: Sturkey. They have gold and platinum disposable blades and they will be happy to send you some free samples. Their web site: www.sturkey.com Phone: 1/800/274-9446 Dolores >>> "Smith, Allen" 1/24/2007 10:06 AM >>> I think she means "Sturkey" (C.L. Sturkey, 834 Cumberland St., Lebanon, PA). I have been very happy with C.L. Sturkey's "gold" blades ever since I switched to disposables. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, January 24, 2007 9:42 AM To: histonet@lists.utsouthwestern.edu; twheelock@mclean.harvard.edu Subject: Re: [Histonet] MICROTOME BLADES We use Stuckey blades and we love them Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From froyer <@t> bitstream.net Wed Jan 24 09:24:15 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Jan 24 09:24:45 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E4030@exchsrv01.barrynet.barry.edu> References: <003201c73fb8$4e99c0a0$c6c401a3@LENOVO27D521E3> <7DBCCC1FBC77C94F99F920D0CA6400B61E4030@exchsrv01.barrynet.barry.edu> Message-ID: <002101c73fcb$b5c38870$7701a80a@Ford> Doesn't any body remember good 'ole lead "L's"? Stainless steel griffon beaker in the oven worked pretty good, but we where in hog heaven with they broke down and got us a "heated paraffin beaker". The beaker itself wasn't actually heated. There was an electrically heated sleeve that the beaker sat in on the bench that kept the paraffin liquefied. I think that it was on of Dr. McCormick's ingenious inventions... How did we EVER survive? ... Open containers of Xylene, Formalin, Asbestos gloves and pads, Lead "L's", and cigarette ash trays located ant each station. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, January 24, 2007 8:59 AM To: Anila Syed Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? I have never felt that embedding stations are worth the counter space they take up. I just barely melt my Paraplast in a Pyrex beaker on a hotplate and maintain its temperature in a 60 degree oven. I set out the mold on the counter and pour in enough Paraplast to quarter fill it. Since the beaker is hot, I keep a cotton glove on my left hand. I leave my right hand bare to handle the forceps.) I warm my forceps in the flame of an alcohol lamp, and position my tissue on the bottom of the mold, set an embedding ring in place, and fill the mold. I wipe the lip of the beaker with a paper towel (usually one that has already been used for drying my hands). Everything except the oven can be put away in a cupboard, leaving me with counter space for other projects. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed Sent: Wednesday, January 24, 2007 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding without a station? Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Jan 24 09:19:28 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 24 09:30:55 2007 Subject: [Histonet] Stains for Lipids In-Reply-To: <920080.65932.qm@web38013.mail.mud.yahoo.com> References: <920080.65932.qm@web38013.mail.mud.yahoo.com> Message-ID: <45B77900.2000503@umdnj.edu> Hi Tamara: The alcohols used to dehydrate tissue for paraffin embedding dissolve out the lipids. Then the clearing agent (xylene) removes any lipids that might have survived the graded alcohols. Once you get to wax there are (almost) no lipids left to stain. You can treat fixed tissue with osmium (dangerous fumes!) to stain the lipids black, then dehydrate and embed but the tissue will be very brittle and hard to cut. Treating fixed tissue with potassium dichromate will retain some lipids but if you really what to know what is there, frozen sections are the only way to go. Frozen sections of fixed (but not dehydrated) material will work. I suggest you get a hold of "Lipid Histochemistry" by Olga Bayliss High. It is #6 in the Microscopy Handbooks series which was published by the Royal Microscopical Society. If you can't find it in the library try a used book dealer. Geoff Tamara Melville wrote: >Hello, > I would like to stain sections of rat aorta and heart for lipids. I am aware that frozen sections are required however in some paraffin sections stained with H&E I saw some structures resembling fat droplets and would like confirmation by using a stain for lipids. I would like to know if there are any methods to stain lipids in paraffin sections. > I would also like to know if tissues fixed and preserved in formal saline can be stained with any of the special lipid stains or are these stains specifically for frozen tissues. > Thanks in advance for any assistance. > > >--------------------------------- >Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Jackie.O'Connor <@t> abbott.com Wed Jan 24 09:35:32 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jan 24 09:36:28 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <002101c73fcb$b5c38870$7701a80a@Ford> Message-ID: It wasn't that long ago - -1989, when I first started working in Honolulu - the contract lab we worked with used ceramic molds with little tags of paper to ID the blocks. They used the now-common plastic cassettes to process, but then threw them away when they embedded. The thinking was that the ceramic molds used less paraffin. What a quality control nightmare that was. Jackie O' "Ford Royer" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 09:24 AM To cc Subject RE: [Histonet] embedding without a station? Doesn't any body remember good 'ole lead "L's"? Stainless steel griffon beaker in the oven worked pretty good, but we where in hog heaven with they broke down and got us a "heated paraffin beaker". The beaker itself wasn't actually heated. There was an electrically heated sleeve that the beaker sat in on the bench that kept the paraffin liquefied. I think that it was on of Dr. McCormick's ingenious inventions... How did we EVER survive? ... Open containers of Xylene, Formalin, Asbestos gloves and pads, Lead "L's", and cigarette ash trays located ant each station. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, January 24, 2007 8:59 AM To: Anila Syed Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? I have never felt that embedding stations are worth the counter space they take up. I just barely melt my Paraplast in a Pyrex beaker on a hotplate and maintain its temperature in a 60 degree oven. I set out the mold on the counter and pour in enough Paraplast to quarter fill it. Since the beaker is hot, I keep a cotton glove on my left hand. I leave my right hand bare to handle the forceps.) I warm my forceps in the flame of an alcohol lamp, and position my tissue on the bottom of the mold, set an embedding ring in place, and fill the mold. I wipe the lip of the beaker with a paper towel (usually one that has already been used for drying my hands). Everything except the oven can be put away in a cupboard, leaving me with counter space for other projects. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed Sent: Wednesday, January 24, 2007 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding without a station? Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jan 24 09:49:00 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jan 24 09:49:14 2007 Subject: [Histonet] pens Message-ID: <0E394B648E5284478A6CCB78E5AFDA270364097C@hpes1.HealthPartners.int> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From POWELL_SA <@t> Mercer.edu Wed Jan 24 09:49:50 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Wed Jan 24 09:50:51 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <002101c73fcb$b5c38870$7701a80a@Ford> Message-ID: <01MCAZF0AXUA8WW0E8@Macon2.Mercer.edu> Me, me, I do and still have a couple, but that tells you that I am older than dirt. :) sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Wednesday, January 24, 2007 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? Doesn't any body remember good 'ole lead "L's"? Stainless steel griffon beaker in the oven worked pretty good, but we where in hog heaven with they broke down and got us a "heated paraffin beaker". The beaker itself wasn't actually heated. There was an electrically heated sleeve that the beaker sat in on the bench that kept the paraffin liquefied. I think that it was on of Dr. McCormick's ingenious inventions... How did we EVER survive? ... Open containers of Xylene, Formalin, Asbestos gloves and pads, Lead "L's", and cigarette ash trays located ant each station. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, January 24, 2007 8:59 AM To: Anila Syed Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? I have never felt that embedding stations are worth the counter space they take up. I just barely melt my Paraplast in a Pyrex beaker on a hotplate and maintain its temperature in a 60 degree oven. I set out the mold on the counter and pour in enough Paraplast to quarter fill it. Since the beaker is hot, I keep a cotton glove on my left hand. I leave my right hand bare to handle the forceps.) I warm my forceps in the flame of an alcohol lamp, and position my tissue on the bottom of the mold, set an embedding ring in place, and fill the mold. I wipe the lip of the beaker with a paper towel (usually one that has already been used for drying my hands). Everything except the oven can be put away in a cupboard, leaving me with counter space for other projects. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed Sent: Wednesday, January 24, 2007 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding without a station? Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = From pkarlisch <@t> psu.edu Wed Jan 24 09:53:49 2007 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Wed Jan 24 09:54:23 2007 Subject: [Histonet] pens In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270364097C@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA270364097C@hpes1.HealthPartners.int> Message-ID: <45B73ABD0200008C00036DF4@GWIA02.HERSHEYMED.NET> Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Jan 24 09:59:14 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jan 24 09:59:46 2007 Subject: [Histonet] pens Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3104@sjhaexc02.sjha.org> We like the ones from Stat Lab - Item SMP-BK - 800-442-3573 Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 10:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From mcauliff <@t> umdnj.edu Wed Jan 24 10:07:14 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 24 10:06:26 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <01MCAZF0AXUA8WW0E8@Macon2.Mercer.edu> References: <01MCAZF0AXUA8WW0E8@Macon2.Mercer.edu> Message-ID: <45B78432.6070203@umdnj.edu> What's an embedding station? When I learned microtechnique we took a small wooden block and folded paper around it to make a box for the wax and tissue. Really! Geoff -----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed >Sent: Wednesday, January 24, 2007 8:05 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] embedding without a station? > >Dear All, > >I have hundreds of carotid plaques to embedd. I have a tissue processor, but > >no embedding station. Would anyone attempt to do this without an embedding >station or do you think I should go and try to find the facilities >somewhere? > >What did people do before embedding stations? > >Many thanks for your input and opinions, > >Anila Syed > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >The information transmitted is intended only for the person or entity to >which it is addressed and may contain confidential, and/or privileged >material. No confidentiality or privilege is waived or lost by any errant >transmission. If you receive this message in error, please immediately >delete it and all copies of it from your system and notify the sender. >E-mail transmission cannot be guaranteed to be secure or error-free as >information could be intercepted, corrupted, lost, destroyed, arrive late or >incomplete, or contain viruses. >Barry University - Miami Shores, FL (http://www.barry.edu) > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >= > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From TJJ <@t> Stowers-Institute.org Wed Jan 24 10:06:18 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jan 24 10:06:42 2007 Subject: [Histonet] Mouse brain atlas Message-ID: Shame on me for not sharing this with the list. I found a wonderful Mouse Brain atlas if any of you are interested. Here is the reference: ISBN 0-12-547637-X The Mouse Brain in Stereotaxic Coordinates George paxinos and Kieth B.J. Franklin Academic Press Additionally I found an image of what this looks like in a gross photograph online here: http://www.mouseatlas.org/data/mouse/libraries/SM202 Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From gentras <@t> vetmed.auburn.edu Wed Jan 24 10:21:45 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jan 24 10:21:59 2007 Subject: [Histonet] Frozen Section H&E's In-Reply-To: <0FDBF29200C637468CCC1F9E7E85A3D2019E3823@MAIL-LR.lha.org> References: <0FDBF29200C637468CCC1F9E7E85A3D2019E3823@MAIL-LR.lha.org> Message-ID: <45B78799.9070903@vetmed.auburn.edu> Hello, I use the following H&E protocol for frozen sections: 1. Stain in Mayer's hematoxylin 5min. 2. Wash in tap H2O 5min. 3. 1% Eosin 3min. 4. Wash in deionized or distilled H2O 3min. 5. Dehydrate in ascending ethanols (ETOH): (x2) 95%, (x2) 100% all 2 min. each (ea.) 6. Clear in (x2) Hemo-de (xylene substitute) 2 min. ea. then xylene 7. Mount in mounting medium of your choice ( I use cryoseal -60) _1% Eosin _Eosin Y 5g. Distilled H2O 750 ml 2% Acetic Acid 10 ml. 100% ETOH 250 ml. Dissolve Eosin in H2O add alcohol & acid. Ready for immediate use. Best wishes, Atoska LeVier, Rebecca J wrote: > Hi Folks, > > I need some help, please. > > Would someone be willing to share reagents and times of the H&E stain > for frozen sections if you are using a xylene substitute? > > We are having a hard time clearing.... > > Thanks so much in advance. > > Becky > > Rebecca J. LeVier BS HT(ASCP) > Histology Supervisor > Email: rjlevier@lancastergeneral.org > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From asmith <@t> mail.barry.edu Wed Jan 24 10:22:00 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jan 24 10:22:11 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <01MCAZF0AXUA8WW0E8@Macon2.Mercer.edu> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4034@exchsrv01.barrynet.barry.edu> I still use lead L's for embedding really large specimens. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Powell Sent: Wednesday, January 24, 2007 10:50 AM To: 'Ford Royer'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? Me, me, I do and still have a couple, but that tells you that I am older than dirt. :) sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Wednesday, January 24, 2007 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? Doesn't any body remember good 'ole lead "L's"? Stainless steel griffon beaker in the oven worked pretty good, but we where in hog heaven with they broke down and got us a "heated paraffin beaker". The beaker itself wasn't actually heated. There was an electrically heated sleeve that the beaker sat in on the bench that kept the paraffin liquefied. I think that it was on of Dr. McCormick's ingenious inventions... How did we EVER survive? ... Open containers of Xylene, Formalin, Asbestos gloves and pads, Lead "L's", and cigarette ash trays located ant each station. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, January 24, 2007 8:59 AM To: Anila Syed Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] embedding without a station? I have never felt that embedding stations are worth the counter space they take up. I just barely melt my Paraplast in a Pyrex beaker on a hotplate and maintain its temperature in a 60 degree oven. I set out the mold on the counter and pour in enough Paraplast to quarter fill it. Since the beaker is hot, I keep a cotton glove on my left hand. I leave my right hand bare to handle the forceps.) I warm my forceps in the flame of an alcohol lamp, and position my tissue on the bottom of the mold, set an embedding ring in place, and fill the mold. I wipe the lip of the beaker with a paper towel (usually one that has already been used for drying my hands). Everything except the oven can be put away in a cupboard, leaving me with counter space for other projects. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed Sent: Wednesday, January 24, 2007 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding without a station? Dear All, I have hundreds of carotid plaques to embedd. I have a tissue processor, but no embedding station. Would anyone attempt to do this without an embedding station or do you think I should go and try to find the facilities somewhere? What did people do before embedding stations? Many thanks for your input and opinions, Anila Syed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From tes4 <@t> u.washington.edu Wed Jan 24 10:28:48 2007 From: tes4 <@t> u.washington.edu (Tiffany Pitts) Date: Wed Jan 24 10:23:03 2007 Subject: [Histonet] Goldner's Trichrome Staining Message-ID: <002c01c73fd4$b983fcc0$4b00a8c0@gucancer.local> Hello and Good Morning all! I hoped not to have to bother you all with this question but an extensive search through the Histonet archives did not turn up the information that I needed so here I am asking for help. My situation is as follows: I have been cutting MMA embedded mouse bones and staining them with a version of Goldner's trichrome stain. I learned this technique from a colleague who has since moved far far away and is no longer in the sciences. I have been merrily chuffing away on my project when I came to a screeching halt two days ago because I have run out of one of the staining reagents. I thought to just look it up on Stains File and get back to business but every protocol I have found so far differs wildly from the protocol I have been following (that has been giving me pretty satisfactory results) In a nut shell here's what I've been doing: Deplasticize rehydrate wash Weigart's Hematoxilyn wash 1% HCl wash Saturated Lithium carbide Sol'n wash "Goldner's Trichrome Stain" reagent (?!?!?) wash for 1 min dehydrate and coverslip It is the "Goldner's Trichrome Stain" reagent that I have run out of and when I looked up my former colleague's notes she has "See Julie" instead of a recipe. Well, Julie is no longer with us either and I am running out of places to look! Can anybody tell me what they think this All-in-one reagent might be? Or perhaps suggest a different solution (pun not intended)? I would be so grateful. Thank you so much, Tiffany Pitts Department of Urology University of Washington Seattle, WA From Annette_hall <@t> pa-ucl.com Wed Jan 24 10:26:23 2007 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Wed Jan 24 10:26:43 2007 Subject: [Histonet] Technical Component Charges for IHC Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5A4B@mercury.pa-ucl.com> Histonetters, I'm in the process of cost accounting our IHC staining. Based on our figures of expenditure vs. revenue, it looks like we are not covering our costs. Would anyone be willing to share their charges for the technical component of their Diagnostic IHC (88342) and Quantitative IHC (88360)? Thanks, Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 x131 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tbraud <@t> holyredeemer.com Wed Jan 24 10:37:09 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jan 24 10:39:19 2007 Subject: [Histonet] Frozen Section H&E's In-Reply-To: <45B78799.9070903@vetmed.auburn.edu> Message-ID: This is what we use at our lab 1. Immerse slides in 95% alcohol in coplin jar for a min of 10 seconds. 2. Rinse gently in running tap water for 10 seconds. 3. Place slides in Richard Allen Hematoxylin 7211 for 40 seconds. 4. Rinse slides in tap water -5 dips. 5. Rinse slides in tap water -5 dips. 6. Wash slides in Bluing Reagent -Shandon Cat.# 9990176 10 dips. 7. Place slides in tap water -5 dips. 8. Place slides in 1% Alcoholic Eosin stain -10 dips. 9. Place slides in 95% alcohol , 2 changes, -5 dips each. 10. Place slides in 100% alcohol, 2 changes, -10 dips each. 11. Place slides in Clear Rite (xylene substitute), 2 changes, -10 dips each. 12. Coverslip slides with permanent mounting media, compatible with Xylene. -Hope it helps Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 LeVier, Rebecca J wrote: > Hi Folks, > > I need some help, please. > > Would someone be willing to share reagents and times of the H&E stain > for frozen sections if you are using a xylene substitute? > > We are having a hard time clearing.... > > Thanks so much in advance. > > Becky > > Rebecca J. LeVier BS HT(ASCP) > Histology Supervisor > Email: rjlevier@lancastergeneral.org > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From PMonfils <@t> Lifespan.org Wed Jan 24 10:50:21 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jan 24 10:50:34 2007 Subject: [Histonet] embedding without a station? In-Reply-To: <003201c73fb8$4e99c0a0$c6c401a3@LENOVO27D521E3> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C02@LSRIEXCH1.lsmaster.lifespan.org> We didn't have embedding stations when I started in histology. We had three stainless steel pitchers of melted paraffin, maybe 500 ml each, in the paraffin oven. We worked on a large, low temperature hotplate, called a "slide warmer". We would take one of the pitchers to the "work station", place it on the slide warmer. We would take the last paraffin container, containing the processed specimens, off the rotary processor and plug it in next to the hotplate. Take an embedding mold (these were made of paper, folded by the histotechs at the end of the previous workday), write the specimen ID on it, place it on the hotplate, pour paraffin into it from the pitcher, unwrap the tissue sample (which were processed wrapped in lens paper - no such thing as cassettes), place it in the mold, then transfer the mold to a metal plate on top of a cakepan full of ice. When the pitcher of paraffin started to get too cool, replace it in the oven and take another pitcher. Sounds pretty primitive - mainly because it was - but it got the job done. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anila Syed > Sent: Wednesday, January 24, 2007 5:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding without a station? > > Dear All, > > I have hundreds of carotid plaques to embedd. I have a tissue processor, but > no embedding station. Would anyone attempt to do this without an embedding > station or do you think I should go and try to find the facilities > somewhere? > > What did people do before embedding stations? > > Many thanks for your input and opinions, > > Anila Syed > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gentras <@t> vetmed.auburn.edu Wed Jan 24 10:55:00 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jan 24 10:55:14 2007 Subject: [Histonet] mouse/rat brain pituitary In-Reply-To: <45B67DD4.5080607@umdnj.edu> References: <45B65FF3.500@vetmed.auburn.edu> <45B67DD4.5080607@umdnj.edu> Message-ID: <45B78F64.3000300@vetmed.auburn.edu> Thanks Geoff, sorry I misconstrued my message a bit I'm aware of the general location of the pituitary, it's just that sometimes we have it upon EM sectioning from mouse and others we don't. Whereas, this is never a problem in cats or dogs ( our primary area of study). I appreciate your assistance. Atoska Geoff McAuliffe wrote: > Hi Atoska: > > The pituitary is not in the brain, it is attached to the ventral > surface by a stalk, the infindibulum. Once the brain is removed, the > stalk breaks and the pituitary is left in the sphenoid bone. If you > cut through the brainstem and carefully lift the brain out of the > skull, you will see the infindibulum just posterior to the optic chiasm. > > Geoff > > Atoska Gentry wrote: > >> Hello, does anyone have info on an atlas/manual in which the >> pituitary of either mouse or rat brain is distinctively displayed? We >> have a research collaborator who is specifically interested in >> studying mouse pituitary. But, we have not been able to find an atlas >> which shows it's exact location in mouse brain. And it is obviously >> not distinguishable upon gross exam. Your prompt replies will be much >> appreciated. Atoska :-) >> > > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From LSebree <@t> uwhealth.org Wed Jan 24 10:56:16 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Jan 24 10:56:26 2007 Subject: [Histonet] MICROTOME BLADES In-Reply-To: <45B76C2A.6040805@mclean.harvard.edu> Message-ID: AccuEdge Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, January 24, 2007 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MICROTOME BLADES Hi Everyone: I am trying other brands of disposable microtome blades. I use Surgipath high profile Teflon coated blades now. What would people recommend? Tim Wheelock Harvard Brain Bank McLean Hospital Belmont , MA 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Jan 24 10:57:51 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jan 24 10:58:06 2007 Subject: [Histonet] Stains for Lipids In-Reply-To: <920080.65932.qm@web38013.mail.mud.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C03@LSRIEXCH1.lsmaster.lifespan.org> The same staining procedures that are used for frozen sections could be used on the paraffin sections, but normally one would not expect to see any lipids in a paraffin section since the tissue has gone through several changes of lipid solvents enroute to paraffin. Yes, tissues fixed in formalin or formol saline can be frozen sectioned and yield excellent lipid stains. Formalin is a good lipid fixative. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tamara Melville > Sent: Tuesday, January 23, 2007 6:30 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Stains for Lipids > > Hello, > I would like to stain sections of rat aorta and heart for lipids. I am aware that frozen sections are required however in some paraffin sections stained with H&E I saw some structures resembling fat droplets and would like confirmation by using a stain for lipids. I would like to know if there are any methods to stain lipids in paraffin sections. > I would also like to know if tissues fixed and preserved in formal saline can be stained with any of the special lipid stains or are these stains specifically for frozen tissues. > Thanks in advance for any assistance. > > > --------------------------------- > Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From funderwood <@t> mcohio.org Wed Jan 24 11:01:30 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed Jan 24 11:02:06 2007 Subject: [Histonet] restoring dried tissue Message-ID: Hi All, I have some dried tissue that needs to be rehydrated before processing. Mentioned in the Histonet archives was using Ruffer's solution, though I couldn't find a recipe. Can someone share this formula with me, or another method for restoring the sample? Thanks in advance, Fred From sbreeden <@t> nmda.nmsu.edu Wed Jan 24 11:03:28 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 24 11:03:41 2007 Subject: [Histonet] Ye Olde Days Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB427@nmdamailsvr.nmda.ad.nmsu.edu> Oh, goody! A Topic! I've been doing this histology thing since 1969 and BOY! have we come a long way! After a 20-year absence from a lab in Albuquerque, I returned to find all these KIDS doing histo - and MEN to boot! Okay, I'd been in the animal research world for a long time... (and Joyce, I worked in Hawaii in 1977 - we could swap tales of adventure!). So the KIDS asked several of us long-in-the-tooth techs how it was in The Old Days. We made up a story about how we had to keep chickens and bees in the back parking lot for the egg albumin (smearing on slides) and the wax. We almost had 'em going for a while there, because as Experienced Professionals, we could make up a really, really good tale! And I remember the "L" brackets, and the asbestos pad and the Lipshaw dispenser and those huge/tall "rings" where you laid the little piece of paper with the accession number. And, Fred, we all had ashtrays on the ledge above our work station. OMG! It's a wonder we're still alive! My old pathologist-boss still smokes and he's close to 90 - and he plays golf and is as sharp as ever. Aaaaahhhh... Ye Goode Olde Days! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From gu.lang <@t> gmx.at Wed Jan 24 11:10:34 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 24 11:11:03 2007 Subject: AW: [Histonet] Goldner's Trichrome Staining In-Reply-To: <002c01c73fd4$b983fcc0$4b00a8c0@gucancer.local> Message-ID: <000601c73fda$90586100$c812a8c0@dielangs.at> Your protocol sounds like an onestep trichrome, but in my literature Goldner consists of more. nuclei-staining with weigert Staining in a mixture of acid fuchsin and Ponceau de Xylidin (0,2 g Ponceau de Xylidin and 0,1 g Acid fuchsin in 300 ml Aqua dest. Plus 0,6 ml 100% acetic acid) Rinse in 1% acetic acid Differentiate in PMA-Orange G solution (few minutes) (3-5g Phosphormolybdic acid and 2 g Orange G in 100 ml Aqua dest) Rinse in 1% acetic acid Counterstain with lightgreen (0,1% 100 ml plus 0,2 ml 100% acetic acid) 5 min 1% acetic acid Dehydrate, clear and mount Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tiffany Pitts Gesendet: Mittwoch, 24. J?nner 2007 17:29 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Goldner's Trichrome Staining Hello and Good Morning all! I hoped not to have to bother you all with this question but an extensive search through the Histonet archives did not turn up the information that I needed so here I am asking for help. My situation is as follows: I have been cutting MMA embedded mouse bones and staining them with a version of Goldner's trichrome stain. I learned this technique from a colleague who has since moved far far away and is no longer in the sciences. I have been merrily chuffing away on my project when I came to a screeching halt two days ago because I have run out of one of the staining reagents. I thought to just look it up on Stains File and get back to business but every protocol I have found so far differs wildly from the protocol I have been following (that has been giving me pretty satisfactory results) In a nut shell here's what I've been doing: Deplasticize rehydrate wash Weigart's Hematoxilyn wash 1% HCl wash Saturated Lithium carbide Sol'n wash "Goldner's Trichrome Stain" reagent (?!?!?) wash for 1 min dehydrate and coverslip It is the "Goldner's Trichrome Stain" reagent that I have run out of and when I looked up my former colleague's notes she has "See Julie" instead of a recipe. Well, Julie is no longer with us either and I am running out of places to look! Can anybody tell me what they think this All-in-one reagent might be? Or perhaps suggest a different solution (pun not intended)? I would be so grateful. Thank you so much, Tiffany Pitts Department of Urology University of Washington Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jan 24 11:16:19 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 24 11:16:41 2007 Subject: AW: [Histonet] restoring dried tissue In-Reply-To: Message-ID: <000701c73fdb$5ef0b710$c812a8c0@dielangs.at> Dried tissue should be restored in Dimethylsulfoxid (DMSO) with 5% water. Never tried personally. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Fred Underwood Gesendet: Mittwoch, 24. J?nner 2007 18:02 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] restoring dried tissue Hi All, I have some dried tissue that needs to be rehydrated before processing. Mentioned in the Histonet archives was using Ruffer's solution, though I couldn't find a recipe. Can someone share this formula with me, or another method for restoring the sample? Thanks in advance, Fred _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Wed Jan 24 11:21:17 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jan 24 11:21:31 2007 Subject: [Histonet] mouse pituitary Message-ID: <45B7958D.70006@vetmed.auburn.edu> Leon, thanks my apologies my wording was a little off. I should have said it's location in relation to mouse brain. Guess I got caught up with the inability to find it *in* the _Atlas of the Mouse Brain and Spinal Cord_ that we have on hand. I'm aware perfectly aware of it's general location. It's just that sometimes it's present in the samples rec'd from necropsy and other's it's not. And our research though primarily small animal does not routinely involve mice or rats. Thanks for your assistance. Atoska Leon Brokken wrote: The pituitary in both mouse and rats is located _under_ the brain, not _in_. It is rather easy to dissect however. If the skull is opened from the top, one can carefully lift the brain out of the skull after disconnecting the optical nerves. The pituitary will remain situated at the bottom of the brain 'cavity' and can then carefully be taken out as a whole. If you need more detailed help on dissection I can provide these (allthough it has been a while ago since I performed these dissections). Cheers, Leon. Atoska Gentry wrote: > Hello, does anyone have info on an atlas/manual in which the pituitary > of either mouse or rat brain is distinctively displayed? We have a > research collaborator who is specifically interested in studying > mouse pituitary. But, we have not been able to find an atlas which > shows it's exact location in mouse brain. And it is obviously not > distinguishable upon gross exam. Your prompt replies will be much > appreciated. Atoska :-) > > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From oshel1pe <@t> cmich.edu Wed Jan 24 11:28:55 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Jan 24 11:29:17 2007 Subject: [Histonet] Ye Olde Days In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB427@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB427@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: You mean you let them believe you were joshing? Heck, honey is a great preservative (ever seen spoiled honey?), although not the best for morphology. The eggshells are a good source of calcium carbonate for various procedures and if they're opened with due care, each egg yields two embedding cups. Then there was the fun of the debates: Rhode Island Red or Bantam or ... ? Phil >Oh, goody! A Topic! I've been doing this histology thing since 1969 >and BOY! have we come a long way! After a 20-year absence from a lab in >Albuquerque, I returned to find all these KIDS doing histo - and MEN to >boot! Okay, I'd been in the animal research world for a long time... >(and Joyce, I worked in Hawaii in 1977 - we could swap tales of >adventure!). So the KIDS asked several of us long-in-the-tooth techs >how it was in The Old Days. We made up a story about how we had to keep >chickens and bees in the back parking lot for the egg albumin (smearing >on slides) and the wax. We almost had 'em going for a while there, >because as Experienced Professionals, we could make up a really, really >good tale! And I remember the "L" brackets, and the asbestos pad and >the Lipshaw dispenser and those huge/tall "rings" where you laid the >little piece of paper with the accession number. And, Fred, we all had >ashtrays on the ledge above our work station. OMG! It's a wonder we're >still alive! My old pathologist-boss still smokes and he's close to 90 >- and he plays golf and is as sharp as ever. Aaaaahhhh... Ye Goode Olde >Days! > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From tracy.bergeron <@t> crl.com Wed Jan 24 11:38:41 2007 From: tracy.bergeron <@t> crl.com (tracy.bergeron@crl.com) Date: Wed Jan 24 11:39:33 2007 Subject: [Histonet] mouse pituitary In-Reply-To: <45B7958D.70006@vetmed.auburn.edu> Message-ID: The best way to ensure you have pituitary present in your samples that come up from necropsy, is to have your necropsy folks open the skull up so formalin can get to the brain, but not remove the brain from the skull. You have a greater chance of keeping the pituitary intact if the brain is removed after fixation. Also for mouse pituitary we have found that because of it's size it can be very easily damaged if removed from the skull for processing. We generally remove the brain, then decalcify the skull with the pituitary in place (formic acid decal solution). Then process, embedd, and cut the xc of skull containing the pituitary. Since switching to this method we rarely have issues with lost or damaged pituitary tissue. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 Atoska Gentry Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 12:21 PM To Histonet cc Subject [Histonet] mouse pituitary Leon, thanks my apologies my wording was a little off. I should have said it's location in relation to mouse brain. Guess I got caught up with the inability to find it *in* the _Atlas of the Mouse Brain and Spinal Cord_ that we have on hand. I'm aware perfectly aware of it's general location. It's just that sometimes it's present in the samples rec'd from necropsy and other's it's not. And our research though primarily small animal does not routinely involve mice or rats. Thanks for your assistance. Atoska Leon Brokken wrote: The pituitary in both mouse and rats is located _under_ the brain, not _in_. It is rather easy to dissect however. If the skull is opened from the top, one can carefully lift the brain out of the skull after disconnecting the optical nerves. The pituitary will remain situated at the bottom of the brain 'cavity' and can then carefully be taken out as a whole. If you need more detailed help on dissection I can provide these (allthough it has been a while ago since I performed these dissections). Cheers, Leon. Atoska Gentry wrote: > Hello, does anyone have info on an atlas/manual in which the pituitary > of either mouse or rat brain is distinctively displayed? We have a > research collaborator who is specifically interested in studying > mouse pituitary. But, we have not been able to find an atlas which > shows it's exact location in mouse brain. And it is obviously not > distinguishable upon gross exam. Your prompt replies will be much > appreciated. Atoska :-) > > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCollins <@t> palmbeachpath.com Wed Jan 24 11:53:58 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Wed Jan 24 11:54:07 2007 Subject: [Histonet] Microwave Tissue Processors In-Reply-To: Message-ID: We have a Peloris processor. Rene is correct. It does not use microwaves. But we love ours. We are an independent pathology laboratory and most of our specimens are small. We are able to process most of our tissues in 1-2 hours AND, in addition, the processing (which does not use xylene) is generally superior to the older, longer processing. It has enabled our laboratory to change the way we work in huge ways. We no longer have the crazy early morning rush to cut all of our blocks because we are able to process many tissues during the morning and afternoon instead of overnight. We embed and cut them during what used to be down times in the afternoon and early evening. It has worked out very well for us. The processor has been very reliable and it saves money on reagents as well. Judy Collins Palm Beach Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zummak Melissa Sent: Tuesday, January 23, 2007 2:50 PM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave Tissue Processors I am in the same boat, we are looking at purchasing new processor(s) very soon to go in the direction of rapid processing and would also like to hear any comments, positive and\or negative about anyone's experience with any model of processor. Anyone had any experience with the Peloris system? Melissa Zummak Alverno Clincal Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, January 23, 2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Tissue Processors Our pathologists are interested in purchasing a microwave tissue processor for smaller biopsies. I was wondering if anyone has any positive and negative feedback. Thanks, Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryakay <@t> shands.ufl.edu Wed Jan 24 11:57:55 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Wed Jan 24 11:58:20 2007 Subject: [Histonet] Microwave Tissue Processors Message-ID: Hi Travis, We have used the Milestone RHS 1& 2 for our biopsies for the past three years and all I can say is it has been a wonderful experience. Our TAT was greatly reduced, the biopsies cut much better (especially the gastric biopsies), the tissue lays flatter and the staining is beautiful. The other benefit was a good reduction in hazardous chemicals as you may only have to use isopropanol and paraffin (if tissues are well fixed). Our pathologists have been extremely happy with the results. We also did correlations with our immunohistochemistry section and have seen no effect on antigen staining. The tissues actually stay on the slides better. Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Rocky Point Laboratories 265-0111, 72093 >>> "Travis Troyer" 1/23/2007 11:04 AM >>> Our pathologists are interested in purchasing a microwave tissue processor for smaller biopsies. I was wondering if anyone has any positive and negative feedback. Thanks, Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Wed Jan 24 12:04:50 2007 From: chapcl <@t> yahoo.com (William Chappell) Date: Wed Jan 24 12:04:56 2007 Subject: [Histonet] Sakura VIP-5 and Alarm System Message-ID: <866756.76950.qm@web37212.mail.mud.yahoo.com> Greetings all, I have been assigned with the tast of finding a good after market alarm system (i.e. phone dialer, etc.) that pairs with our brand new Sakura VIP-5. Any ideas? Any Favorites? Any that just don't work right? Thanks, Will From tracy.bergeron <@t> crl.com Wed Jan 24 12:04:18 2007 From: tracy.bergeron <@t> crl.com (tracy.bergeron@crl.com) Date: Wed Jan 24 12:05:07 2007 Subject: [Histonet] mouse pituitary In-Reply-To: Message-ID: I did forget to mention we do this with Rat pituitary as well. Someone asked me about trimming. Once the skull is sufficiently decaled that it can be cut with a blade easily we trim away the excess skull, so that the only portion remaining is the xc containing the pituitary gland. Hope this is helpful. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 tracy.bergeron@crl.com Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 12:38 PM To histonet@pathology.swmed.edu cc Subject Re: [Histonet] mouse pituitary The best way to ensure you have pituitary present in your samples that come up from necropsy, is to have your necropsy folks open the skull up so formalin can get to the brain, but not remove the brain from the skull. You have a greater chance of keeping the pituitary intact if the brain is removed after fixation. Also for mouse pituitary we have found that because of it's size it can be very easily damaged if removed from the skull for processing. We generally remove the brain, then decalcify the skull with the pituitary in place (formic acid decal solution). Then process, embedd, and cut the xc of skull containing the pituitary. Since switching to this method we rarely have issues with lost or damaged pituitary tissue. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 Atoska Gentry Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 12:21 PM To Histonet cc Subject [Histonet] mouse pituitary Leon, thanks my apologies my wording was a little off. I should have said it's location in relation to mouse brain. Guess I got caught up with the inability to find it *in* the _Atlas of the Mouse Brain and Spinal Cord_ that we have on hand. I'm aware perfectly aware of it's general location. It's just that sometimes it's present in the samples rec'd from necropsy and other's it's not. And our research though primarily small animal does not routinely involve mice or rats. Thanks for your assistance. Atoska Leon Brokken wrote: The pituitary in both mouse and rats is located _under_ the brain, not _in_. It is rather easy to dissect however. If the skull is opened from the top, one can carefully lift the brain out of the skull after disconnecting the optical nerves. The pituitary will remain situated at the bottom of the brain 'cavity' and can then carefully be taken out as a whole. If you need more detailed help on dissection I can provide these (allthough it has been a while ago since I performed these dissections). Cheers, Leon. Atoska Gentry wrote: > Hello, does anyone have info on an atlas/manual in which the pituitary > of either mouse or rat brain is distinctively displayed? We have a > research collaborator who is specifically interested in studying > mouse pituitary. But, we have not been able to find an atlas which > shows it's exact location in mouse brain. And it is obviously not > distinguishable upon gross exam. Your prompt replies will be much > appreciated. Atoska :-) > > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jan 24 12:05:12 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jan 24 12:05:25 2007 Subject: [Histonet] CPT Code for preparing on slide Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F44D@lmhsmail.lmhealth.org> We may start doing some work for an outside office and I need to create a new billable test. We would be processing blocks, embedding, cutting, and staining for H&E. Could anyone share with me what CPT code you use or how you bill it? Thanks so much. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From syedab <@t> totalise.co.uk Wed Jan 24 12:12:16 2007 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Wed Jan 24 12:11:11 2007 Subject: [Histonet] embedding without a station? References: <4EBFF65383B74D49995298C4976D1D5E273C02@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <009b01c73fe3$2f525740$42640a52@pbn> Wow!! Thanks for all the suggestions and trips down memory lane! I dont get how paper could have been used as moulds!! and I love the teapot idea!! As for the asbestos and lead etc, I won't mention any of that to our safety officer :) Thanks everyone, I will try to go with a beaker in the incubator and a hotplate, but will also try the doughnuts approach :) Thanks Anila ----- Original Message ----- From: "Monfils, Paul" To: Sent: Wednesday, January 24, 2007 4:50 PM Subject: RE: [Histonet] embedding without a station? We didn't have embedding stations when I started in histology. We had three stainless steel pitchers of melted paraffin, maybe 500 ml each, in the paraffin oven. We worked on a large, low temperature hotplate, called a "slide warmer". We would take one of the pitchers to the "work station", place it on the slide warmer. We would take the last paraffin container, containing the processed specimens, off the rotary processor and plug it in next to the hotplate. Take an embedding mold (these were made of paper, folded by the histotechs at the end of the previous workday), write the specimen ID on it, place it on the hotplate, pour paraffin into it from the pitcher, unwrap the tissue sample (which were processed wrapped in lens paper - no such thing as cassettes), place it in the mold, then transfer the mold to a metal plate on top of a cakepan full of ice. When the pitcher of paraffin started to get too cool, replace it in the oven and take another pitcher. Sounds pretty primitive - mainly because it was - but it got the job done. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anila Syed > Sent: Wednesday, January 24, 2007 5:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding without a station? > > Dear All, > > I have hundreds of carotid plaques to embedd. I have a tissue processor, but > no embedding station. Would anyone attempt to do this without an embedding > station or do you think I should go and try to find the facilities > somewhere? > > What did people do before embedding stations? > > Many thanks for your input and opinions, > > Anila Syed > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.17.8/649 - Release Date: 1/23/07 From mtitford <@t> aol.com Wed Jan 24 12:20:49 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jan 24 12:21:07 2007 Subject: [Histonet] Fat stains on P/S. Message-ID: <8C90DFB81F67059-137C-11F5@WEBMAIL-MA20.sysops.aol.com> Tam Melville asks about fat stains on paraffin sections. Some lipids do remain in paraffin sections although most are removed by the organic solvents used in processing. I do not think any "fat droplets" would survive paraffin processing. Compound lipids, like sphingomyelin do survive and can be demonstrated by Sudan black, but the whole thing is less than perfect. They stain a kind of grey color. McManus published a method in which you take P/S sections to 70% alcohol, stain in sat. Sudan black in 70% alcohol for 30 min to 3 hours at 60oC, rinse in 70% alcohol, and then counterstain and mount in aquous mounting medium. I don't have the reference, but it is on page 302 of the 1968 edition of "Handbook of histopathological techniques" by C.F.A. Culling. We do it occasionally when a resident says "D***, I should have done a F/S fat stain on this" Michael Titford Pathology USA Mobile AL ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Jann.Nevard <@t> baycare.org Wed Jan 24 13:09:31 2007 From: Jann.Nevard <@t> baycare.org (Nevard, Jann) Date: Wed Jan 24 13:17:19 2007 Subject: [Histonet] HOSTOLOGIST Opportunities with BayCare Health System Message-ID: <98ECE5B11C59DE449F8949A45C52A8190400F95A@EXCHANGE02-VS.BCAD.BAYCARE.ORG> JOB POSTING FOR BAYCARE HEALTH SYSTEM TAMPA BAY AREA FLORIDA We are the 5th largest not-for-profit health care system in the U.S. and the largest on Florida's West Coast. Our nine area hospitals are leaders in research and teaching, and have been ranked among the nation's best health care providers. We can offer full time, part time or pool. Feel free to visit us on the web at www.baycarejobs.com. If you would like to hear more, please contact me below to set up a telephonic interview. The Histologist is responsible for all phases of tissue processing from fixation to final slide, including special stains and techniques; maintains all files, records, and documentation required by state and federal licensing boards; cleans and maintains equipment and instruments; stocks and maintains supplies and performs other duties as assigned. *Florida Licensure, 2+ years routine and special Histology experience. Work occasional weekend. Education: High school or equivalent Required RELATED FIELD AS Preferred Experience: One year LABORATORY Preferred Licensure: HISTOLOG Required ASCP Preferred CLS Preferred NCA Preferred Specific Skills Required: Computer skill appropriate to position Organizational skills Time management Work independently with minimal supervision Teamwork Interpersonal skills Medical terminology use and understanding Written and verbal communication skills Customer service skills Currently I have needs for day shift and night shift. We offer full benefits with matching 401K, generous relocation allowance, top 100 hospitals, great locations. Please call me at the numbers. Jann Nevard, CIR Regional Source Recruiter BayCare Health System Tel: 727-519-1315 Fax: 727-519-1323 Toll free: 1-866-221-3222 option 1 Web:www.baycarejobs.com e-mail: jann.nevard@baycare.org Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From tbraud <@t> holyredeemer.com Wed Jan 24 13:18:49 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jan 24 13:20:50 2007 Subject: [Histonet] CPT Code for preparing on slide In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F44D@lmhsmail.lmhealth.org> Message-ID: Ahhhh.....the curse of the Histo billed tests. The CPT code for Processing, embedding, cutting and H&e's is based solely on the diagnosis of the type of tissue, per specimen, regardless of the number of blocks and slides produced. If you are doing this work to be read elsewhere, you might do better to come up with a contracted price, billed through separate invoices. Your financial folks should be able to add to capture your billed tests if you are worried about showing workload. If your pathologists are reading the slides, then you will have to bill based on diagnosis. You can arrange discounted pricing on current pricing through contracts from your hospital billing (if that's the route it takes) If I can help you negotiate through this, please just give me a call. Good Luck, T Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Wednesday, January 24, 2007 1:05 PM To: histonet@pathology.swmed.edu Subject: [Histonet] CPT Code for preparing on slide We may start doing some work for an outside office and I need to create a new billable test. We would be processing blocks, embedding, cutting, and staining for H&E. Could anyone share with me what CPT code you use or how you bill it? Thanks so much. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From lblazek <@t> digestivespecialists.com Wed Jan 24 13:20:30 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jan 24 13:20:54 2007 Subject: [Histonet] Ye Olde Days Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684CDA@bruexchange.digestivespecialists.com> Sally, Good grief you're old! But then of course I remember all of those things also so I guess we're not all that old if we can still remember them! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 24, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ye Olde Days Oh, goody! A Topic! I've been doing this histology thing since 1969 and BOY! have we come a long way! After a 20-year absence from a lab in Albuquerque, I returned to find all these KIDS doing histo - and MEN to boot! Okay, I'd been in the animal research world for a long time... (and Joyce, I worked in Hawaii in 1977 - we could swap tales of adventure!). So the KIDS asked several of us long-in-the-tooth techs how it was in The Old Days. We made up a story about how we had to keep chickens and bees in the back parking lot for the egg albumin (smearing on slides) and the wax. We almost had 'em going for a while there, because as Experienced Professionals, we could make up a really, really good tale! And I remember the "L" brackets, and the asbestos pad and the Lipshaw dispenser and those huge/tall "rings" where you laid the little piece of paper with the accession number. And, Fred, we all had ashtrays on the ledge above our work station. OMG! It's a wonder we're still alive! My old pathologist-boss still smokes and he's close to 90 - and he plays golf and is as sharp as ever. Aaaaahhhh... Ye Goode Olde Days! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Jan 24 13:23:12 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jan 24 13:23:20 2007 Subject: [Histonet] embedding without a station? Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684CDB@bruexchange.digestivespecialists.com> Someone needs to teach Anila how to make a boat! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila Syed Sent: Wednesday, January 24, 2007 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] embedding without a station? Wow!! Thanks for all the suggestions and trips down memory lane! I dont get how paper could have been used as moulds!! and I love the teapot idea!! As for the asbestos and lead etc, I won't mention any of that to our safety officer :) Thanks everyone, I will try to go with a beaker in the incubator and a hotplate, but will also try the doughnuts approach :) Thanks Anila ----- Original Message ----- From: "Monfils, Paul" To: Sent: Wednesday, January 24, 2007 4:50 PM Subject: RE: [Histonet] embedding without a station? We didn't have embedding stations when I started in histology. We had three stainless steel pitchers of melted paraffin, maybe 500 ml each, in the paraffin oven. We worked on a large, low temperature hotplate, called a "slide warmer". We would take one of the pitchers to the "work station", place it on the slide warmer. We would take the last paraffin container, containing the processed specimens, off the rotary processor and plug it in next to the hotplate. Take an embedding mold (these were made of paper, folded by the histotechs at the end of the previous workday), write the specimen ID on it, place it on the hotplate, pour paraffin into it from the pitcher, unwrap the tissue sample (which were processed wrapped in lens paper - no such thing as cassettes), place it in the mold, then transfer the mold to a metal plate on top of a cakepan full of ice. When the pitcher of paraffin started to get too cool, replace it in the oven and take another pitcher. Sounds pretty primitive - mainly because it was - but it got the job done. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anila Syed > Sent: Wednesday, January 24, 2007 5:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding without a station? > > Dear All, > > I have hundreds of carotid plaques to embedd. I have a tissue processor, but > no embedding station. Would anyone attempt to do this without an embedding > station or do you think I should go and try to find the facilities > somewhere? > > What did people do before embedding stations? > > Many thanks for your input and opinions, > > Anila Syed > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.17.8/649 - Release Date: 1/23/07 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Jan 24 13:37:33 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Jan 24 13:37:43 2007 Subject: [Histonet] RE: embedding without a station? References: <5.2.1.1.2.20070124085612.01fd3a18@mailhost.ces.clemson.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A11FFE5@uwhis-xchng3.uwhis.hosp.wisc.edu> Once again thank you all for the very interesting stories about the old(er) days. I always like collecting your combined wisdom "Just in case". I have only been in the field for 5 years. I am the only trained histotech in our lab, so I frequently have to help the others with special stains troubleshooting, etc. I feel like they think I know everything abjout histology (almost). I am glad I have somewhere to turn to to put me back in my place once in a while. (and get help if I need it). I have known for a long time how spoiled the current bunch of histotechs coming in to the field are. Thanks again for the unending wisdom. Don't retire too soon either! Claire Ingles UW Hospital and Clinics Madison WI P.S. Anybody thought of starting a Histology history book? From RSRICHMOND <@t> aol.com Wed Jan 24 13:43:36 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Jan 24 13:43:48 2007 Subject: [Histonet] Re: embedding without a station? Message-ID: I've certainly seen an electric fry pan used in place of an embedding station, at a "little histology lab on the prairie" - an ordinary house used as a histology lab (I've seen at least three of these), no ventilation for the formaldehyde, and God help everybody if the open bucket Technicon catches fire. Meanwhile, I suppose, the department of radiology was doing mammograms with the X-rays emitted from the back end of an old color TV set? The red-haired stepchild again. Bob Richmond Samurai Pathologist Knoxville TN From Jackie.O'Connor <@t> abbott.com Wed Jan 24 13:45:28 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jan 24 13:46:04 2007 Subject: [Histonet] Ye Olde Days In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684CDA@bruexchange.digestivespecialists.com> Message-ID: We're not OLD - we're well fixed. "Blazek, Linda" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 01:20 PM To "Breeden, Sara" , cc Subject RE: [Histonet] Ye Olde Days Sally, Good grief you're old! But then of course I remember all of those things also so I guess we're not all that old if we can still remember them! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 24, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ye Olde Days Oh, goody! A Topic! I've been doing this histology thing since 1969 and BOY! have we come a long way! After a 20-year absence from a lab in Albuquerque, I returned to find all these KIDS doing histo - and MEN to boot! Okay, I'd been in the animal research world for a long time... (and Joyce, I worked in Hawaii in 1977 - we could swap tales of adventure!). So the KIDS asked several of us long-in-the-tooth techs how it was in The Old Days. We made up a story about how we had to keep chickens and bees in the back parking lot for the egg albumin (smearing on slides) and the wax. We almost had 'em going for a while there, because as Experienced Professionals, we could make up a really, really good tale! And I remember the "L" brackets, and the asbestos pad and the Lipshaw dispenser and those huge/tall "rings" where you laid the little piece of paper with the accession number. And, Fred, we all had ashtrays on the ledge above our work station. OMG! It's a wonder we're still alive! My old pathologist-boss still smokes and he's close to 90 - and he plays golf and is as sharp as ever. Aaaaahhhh... Ye Goode Olde Days! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Wed Jan 24 13:46:36 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed Jan 24 13:46:50 2007 Subject: [Histonet] mouse pituitary In-Reply-To: Message-ID: <946263.75507.qm@web50308.mail.yahoo.com> It is possible to safely remove the fixed pituitary from the skull--it justs takes a bit of care and one of the microscalpels that one can get from Roboz (I prefer those--no interest other than that). However (and it's a big one) there is always the possibility of losing the pit during processing into paraffin--even using biopsy cassettes for processing. I have wrapped the pit in lens paper (use the Ross lens tissue only) to ensure not losing the pit. However, I have also done the decal route (we use a slow decal called CalRite from Richard Allan--again no interest just that the product works well and preserve soft tissue while giving excellent decalcification results) that Tracy uses. I agree wholeheartedly with not removing the fresh pit from the skull--almost guaranteed damage and possible loss. Roger Moretz, Ph.D. BI Pharmaceuticals Ridgefield, CT --- tracy.bergeron@crl.com wrote: > I did forget to mention we do this with Rat > pituitary as well. > > Someone asked me about trimming. Once the skull > is sufficiently decaled > that it can be cut with a blade easily we trim away > the excess skull, so > that the only portion remaining is the xc containing > the pituitary gland. > > Hope this is helpful. > > Tracy E. Bergeron, BS, HT, HTL (ASCP) > Histotechnologist > Charles River Laboratories > Wilmington, MA > 978-658-6000 x 1229 > > > > tracy.bergeron@crl.com > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/24/2007 12:38 PM > > To > histonet@pathology.swmed.edu > cc > > Subject > Re: [Histonet] mouse pituitary > > > > > > > > The best way to ensure you have pituitary > present in your samples > that come up from necropsy, is to have your necropsy > folks open the skull > up so formalin can get to the brain, but not remove > the brain from the > skull. You have a greater chance of keeping the > pituitary intact if the > brain is removed after fixation. > > Also for mouse pituitary we have found that > because of it's size > it can be very easily damaged if removed from the > skull for processing. We > > generally remove the brain, then decalcify the skull > with the pituitary in > > place (formic acid decal solution). Then process, > embedd, and cut the xc > of skull containing the pituitary. Since switching > to this method we > rarely have issues with lost or damaged pituitary > tissue. > > Tracy E. Bergeron, BS, HT, HTL (ASCP) > Histotechnologist > Charles River Laboratories > Wilmington, MA > 978-658-6000 x 1229 > > > > Atoska Gentry > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/24/2007 12:21 PM > > To > Histonet > cc > > Subject > [Histonet] mouse pituitary > > > > > > > > Leon, thanks my apologies my wording was a little > off. I should have > said it's location in relation to mouse brain. Guess > I got caught up > with the inability to find it *in* the _Atlas of the > Mouse Brain and > Spinal Cord_ that we have on hand. I'm aware > perfectly aware of it's > general location. It's just that sometimes it's > present in the samples > rec'd from necropsy and other's it's not. And our > research though > primarily small animal does not routinely involve > mice or rats. Thanks > for your assistance. Atoska > Leon Brokken wrote: > > The pituitary in both mouse and rats is located > _under_ the brain, not > _in_. It is rather easy to dissect however. If the > skull is opened from > the top, one can carefully lift the brain out of the > skull after > disconnecting the optical nerves. The pituitary will > remain situated at > the bottom of the brain 'cavity' and can then > carefully be taken out as > a whole. > > If you need more detailed help on dissection I can > provide these > (allthough it has been a while ago since I performed > these dissections). > > Cheers, Leon. > > Atoska Gentry wrote: > > > > Hello, does anyone have info on an atlas/manual in > which the pituitary > > of either mouse or rat brain is distinctively > displayed? We have a > > research collaborator who is specifically > interested in studying > > mouse pituitary. But, we have not been able to > find an atlas which > > shows it's exact location in mouse brain. And it > is obviously not > > distinguishable upon gross exam. Your prompt > replies will be much > > appreciated. Atoska :-) > > > > > -- > Atoska S. Gentry, B.S., HT(ASCP) > Research Assistant IV > Scott-Ritchey RSCH Center > College of Vet. Med > Auburn, AL 36849 > PH (334) 844-5579 > FAX (334) 844-5850 > email: gentras@vetmed.auburn.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091 From froyer <@t> bitstream.net Wed Jan 24 14:03:42 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Jan 24 14:04:14 2007 Subject: [Histonet] Ye Olde Days In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684CDA@bruexchange.digestivespecialists.com> References: <6CBA6DC98A079D408C87250591D9DFB802684CDA@bruexchange.digestivespecialists.com> Message-ID: <000d01c73ff2$bf972e20$7701a80a@Ford> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, January 24, 2007 1:21 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ye Olde Days Sally, Good grief you're old! But then of course I remember all of those things also so I guess we're not all that old if we can still remember them! Linda ------------------ Remembering 1969 is EASY! Remembering 1969 is NOT a problem. What is hard these days is trying to remember where I parked the car when I leave work, or (if I get that far)... now where did I put my car keys?! ~ Ford From JMacDonald <@t> mtsac.edu Wed Jan 24 14:11:56 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 24 14:12:04 2007 Subject: [Histonet] Re: embedding without a station? In-Reply-To: Message-ID: I worked at a large hospital in Toronto in the 80s and they were using electric griddles for the embedding, along with metal grids and L brackets for the larger specimens. The labels were small slips of paper tucked in the "block". When the blocks set up they were dumped into a sink of ice water to cool them. Jennifer MacDonald RSRICHMOND@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 11:43 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: embedding without a station? I've certainly seen an electric fry pan used in place of an embedding station, at a "little histology lab on the prairie" - an ordinary house used as a histology lab (I've seen at least three of these), no ventilation for the formaldehyde, and God help everybody if the open bucket Technicon catches fire. Meanwhile, I suppose, the department of radiology was doing mammograms with the X-rays emitted from the back end of an old color TV set? The red-haired stepchild again. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KPierce <@t> cancer-test.com Wed Jan 24 14:15:33 2007 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Wed Jan 24 14:15:43 2007 Subject: [Histonet] histology position in Seattle Message-ID: <9380B79A09A6DD43884D977256FC12020B0633@fleming.mla.local> We have a position available for a day shift histotech at a small reference lab in Seattle Wa coming available in the next month. Position is full time, 6 AM to 2 PM, Mon-Fri. Contact Ken Pierce at kpierce@cancer-test.com for details. From RSRICHMOND <@t> aol.com Wed Jan 24 14:29:08 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Jan 24 14:29:27 2007 Subject: [Histonet] Re: Goldner's Trichrome Staining Message-ID: Tiffany Pitts (University of Washington state) is trying to reconstruct a "Goldner trichrome stain", and Gudrun Lang (Analytikerin in Linz, Germany) assists. (Some history may help - see below.)- I've reproduced their formulas, standardizing English spellings for the convenience of the Googler: Deplasticize, rehydrate, wash stain nuclei in Weigert's hematoxylin wash, 1% HCl, wash blue in saturated lithium carbonate wash stain in "Goldner's Trichrome Stain" reagent (?!?!?) wash for 1 min, dehydrate and coverslip It is the "Goldner's Trichrome Stain" reagent that I have run out of and when I looked up my former colleague's notes she has "See Julie" instead of a recipe. Well, Julie is no longer with us either and I am running out of places to look! nuclei-staining with Weigert hematoxylin Staining in a mixture of acid fuchsin (or fuchsine) and ponceau de xylidine (0.2 g ponceau de xylidine and 0.1 g acid fuchsin in 300 mL distilled water with 0.6 ml 100% [glacial] acetic acid) Rinse in 1% acetic acid Stain in PMA-Orange G solution (few minutes) (3-5g phosphomolybdic acid and 2 g Orange G in 100 mL distilled water) Rinse in 1% acetic acid Counterstain with Light Green SF (0.1% 100 mL plus 0.2 mL 100% acetic acid) 5 min 1% acetic acid Dehydrate, clear and mount ***************** I probably have more information, but I'm away from my books this week. You may have difficulty in obtaining some of these dyes, particularly ponceau de xylidine (probably the same as ponceau red). Pierre Masson in Montreal (1920's - 1930's) is supposed to have developed many variants of his trichrome stain, and there is no one "Masson stain". Goldner's variant, which Gudrun Lang describes, was taken up by Chandler Foot at Co rnell Medical Center/New York Hospital in the 1930's as a general oversight stain in place of H & E, and the stain was sometimes called the Goldner-Foot stain. Chandler Foot, one of the founders of American surgical pathology, died in 1948. Most of his slides were destroyed in a management disaster around 1960, but some still survived in teaching collections when I was a resident there in 1968. - George Papanicolaou, at Cornell around 1940, probably used this stain as the basis of the still-used "Pap stain", though certain historical proof of this point is probably lacking (Gary Gill, do you know?). I would suggest a modern green trichrome stain - they're available commercially, though most pathologists have abandoned them because they aren't good for liver biopsies. I'd advise separating the green dye and the orange G, but in order to reproduce your present stain, you may need to seek out or prepare a stain mixture that combines them. Bob Richmond Samurai Pathologist Knoxville TN From jwatson <@t> gnf.org Wed Jan 24 15:02:28 2007 From: jwatson <@t> gnf.org (James Watson) Date: Wed Jan 24 15:02:40 2007 Subject: [Histonet] Beta Gal staining on formalin fixed mouse embryo's Message-ID: Does anyone have a protocol for Beta Gal staining on formalin fixed tissue, either frozen sections or paraffin. Thank you. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Jan 24 15:31:32 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Jan 24 15:35:47 2007 Subject: [Histonet] Tennessee Society for Histotechnology Call for abstracts Message-ID: <898D946569A27444B65667A49C074052D0DFC3@mailbe06.mc.vanderbilt.edu> The annual TSH meeting will be held June 7,8,9 at the Renaissance Center in Dickson, TN. The Renaissance Center is an arts and technology education and performing arts center just 35 miles west of Nashville on Interstate 40. We are currently seeking presenters. Please contact me if you are interested in presenting at our meeting or if you have questions or comments. Thanks and have a great rest of the week! Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 phone (615) 343-7089 fax TSH President NSH Quality Control Committee Chair From jnocito <@t> satx.rr.com Wed Jan 24 17:06:24 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 24 17:06:43 2007 Subject: [Histonet] Ye Olde Days References: <6CBA6DC98A079D408C87250591D9DFB802684CDA@bruexchange.digestivespecialists.com> <000d01c73ff2$bf972e20$7701a80a@Ford> Message-ID: <00b201c7400c$45a5afa0$649eae18@yourxhtr8hvc4p> the kids that work for me only saw steel blades in text books. When I told them that I used to strop my own knives, they had to look up the "strop" in the dictionary. I remember smoking while coverslipping and having a cigarette in the morgue during an autopsy to get the smell out. Of course, that was before MSDS, lab safety, and jet airplanes. Ahhhh, the good times Joe ----- Original Message ----- From: "Ford Royer" To: Sent: Wednesday, January 24, 2007 2:03 PM Subject: RE: [Histonet] Ye Olde Days > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Wednesday, January 24, 2007 1:21 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ye Olde Days > > Sally, Good grief you're old! But then of course I remember all of > those things also so I guess we're not all that old if we can still > remember them! > > Linda > > > ------------------ > Remembering 1969 is EASY! Remembering 1969 is NOT a problem. > > What is hard these days is trying to remember where I parked the car when > I > leave work, or (if I get that far)... now where did I put my car keys?! > > ~ Ford > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrsseagle <@t> yahoo.com Wed Jan 24 16:51:45 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Wed Jan 24 17:18:34 2007 Subject: [Histonet] Microtome Blades Message-ID: <947051.75167.qm@web51812.mail.yahoo.com> I AGREE YOU JUST CANY HARDLY BEAT ACCUEDGE BLADES Michelle Seagle HT (ASCP) Rutherford Hospital Rutherfordton NC MICHELLE SEAGLE --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From bhewlett <@t> cogeco.ca Wed Jan 24 17:57:36 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed Jan 24 17:57:45 2007 Subject: [Histonet] Re: Goldner's Trichrome Staining References: Message-ID: <000e01c74013$6c7f3220$6500a8c0@mainbox> Hi Bob, Interesting background. All of these dyes are currently available! The problem in obtaining them may be the use of various common names to describe them. The use of the listed name and the CI # and CI name indicates a specific dye, synonyms may be confusing since they can be shared with other non-related dyes. The following is from the latest edition of Conn's Biological Stains. Acid fuchsine, CI 42685, CI Acid violet 19, synonym: acid magenta. Commonly available. Ponceau de xylidine is still available from a number of suppliers. Listed as Ponceau 2R, CI 16150, CI Acid red 26, synonym: ponceau de xylidine. The US Sigma catalogue # is P2395. Orange G, CI 16230, CI Acid orange 10, synonym: wool orange 2G. Commonly available. Light green SF, CI 42095, CI Acid green 5, synonym: light green SF yellowish. Commonly available. Best regards, Bryan ----- Original Message ----- From: To: Sent: Wednesday, January 24, 2007 3:29 PM Subject: [Histonet] Re: Goldner's Trichrome Staining > Tiffany Pitts (University of Washington state) is trying to reconstruct a > "Goldner trichrome stain", and Gudrun Lang (Analytikerin in Linz, Germany) > assists. (Some history may help - see below.)- I've reproduced their > formulas, > standardizing English spellings for the convenience of the Googler: > > Deplasticize, rehydrate, wash > stain nuclei in Weigert's hematoxylin > wash, 1% HCl, wash > blue in saturated lithium carbonate > wash > stain in "Goldner's Trichrome Stain" reagent (?!?!?) > wash for 1 min, dehydrate and coverslip > > It is the "Goldner's Trichrome Stain" reagent that I have run out of and > when > I looked up my former colleague's notes she has "See Julie" instead of a > recipe. Well, Julie is no longer with us either and I am running out of > places to > look! > > nuclei-staining with Weigert hematoxylin > Staining in a mixture of acid fuchsin (or fuchsine) and ponceau de > xylidine > (0.2 g ponceau de xylidine and 0.1 g acid fuchsin in 300 mL distilled > water > with > 0.6 ml 100% [glacial] acetic acid) > Rinse in 1% acetic acid > Stain in PMA-Orange G solution (few minutes) > (3-5g phosphomolybdic acid and 2 g Orange G in 100 mL distilled water) > Rinse in 1% acetic acid > Counterstain with Light Green SF (0.1% 100 mL plus 0.2 mL 100% acetic > acid) > 5 min 1% acetic acid > Dehydrate, clear and mount > ***************** > I probably have more information, but I'm away from my books this week. > You > may have difficulty in obtaining some of these dyes, particularly ponceau > de > xylidine (probably the same as ponceau red). > > Pierre Masson in Montreal (1920's - 1930's) is supposed to have developed > many variants of his trichrome stain, and there is no one "Masson stain". > Goldner's variant, which Gudrun Lang describes, was taken up by Chandler > Foot at Co > rnell Medical Center/New York Hospital in the 1930's as a general > oversight > stain in place of H & E, and the stain was sometimes called the > Goldner-Foot > stain. Chandler Foot, one of the founders of American surgical pathology, > died in > 1948. Most of his slides were destroyed in a management disaster around > 1960, > but some still survived in teaching collections when I was a resident > there in > 1968. - George Papanicolaou, at Cornell around 1940, probably used this > stain > as the basis of the still-used "Pap stain", though certain historical > proof of > this point is probably lacking (Gary Gill, do you know?). > > I would suggest a modern green trichrome stain - they're available > commercially, though most pathologists have abandoned them because they > aren't good for > liver biopsies. I'd advise separating the green dye and the orange G, but > in > order to reproduce your present stain, you may need to seek out or prepare > a > stain mixture that combines them. > > Bob Richmond > Samurai Pathologist > Knoxville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LenaSpencer <@t> insightbb.com Wed Jan 24 19:42:34 2007 From: LenaSpencer <@t> insightbb.com (Lena Spencer) Date: Wed Jan 24 19:42:19 2007 Subject: [Histonet] Bar Code Labels Message-ID: <000601c74022$165c4540$6401a8c0@DCXBDY81> Hi If there is anyone out there who has a Ventana Es and would like some bar code labels let me know. We no longer have the ES and are willing to give these to someone who could use them. Please e-mail me off line if you are interested. Lena From webb3655 <@t> sbcglobal.net Wed Jan 24 21:12:19 2007 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Wed Jan 24 21:12:27 2007 Subject: [Histonet] Robert Lott Message-ID: <20070125031219.93304.qmail@web83115.mail.mud.yahoo.com> Greetings, Does anyone have a phone number or email for Robert Lott? Thanks, Judy (Webb) McKinney 817-927-1024 jwebb01@jpshealth.org From YJONES2 <@t> csmlab.com Thu Jan 25 00:47:16 2007 From: YJONES2 <@t> csmlab.com (Yvonne Jones) Date: Thu Jan 25 00:50:02 2007 Subject: [Histonet] MICROTOME BLADES In-Reply-To: References: <45B76C2A.6040805@mclean.harvard.edu> Message-ID: <45B80C240200004F0000051D@GWGATE1.ahm.com> I know that I'm going to cause a tidal wave, but actually I have recently gotten a new microtome (Finesse 325) and I have had better results with the Dura-Edge High Profile blades. Added benefit, they're cheaper!! >>> "Sebree Linda A." 01/24/2007 11:56 AM >>> AccuEdge Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, January 24, 2007 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MICROTOME BLADES Hi Everyone: I am trying other brands of disposable microtome blades. I use Surgipath high profile Teflon coated blades now. What would people recommend? Tim Wheelock Harvard Brain Bank McLean Hospital Belmont , MA 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet----------------------------------------- From d.a.faichney <@t> stir.ac.uk Thu Jan 25 04:41:22 2007 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Thu Jan 25 04:41:57 2007 Subject: [Histonet] Malt diastase Message-ID: <38E5B431A5228D46A9A99E0206D5788317CEA5@medwin.ad.stir.ac.uk> Dear all, I need to obtain malt diastase for Periodic acid schiff staining for glycogen in blood smears. I am finding this difficult (in the UK) and would like to know from any carbohydate people out there what I could use instead. Thanks Debbie Faichney Institute of Aquaculture Stirling, Scotland -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. From billingconsultants <@t> yahoo.com Thu Jan 25 04:45:20 2007 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Thu Jan 25 04:45:28 2007 Subject: [Histonet] Re: CPT code Message-ID: <456811.36636.qm@web54208.mail.yahoo.com> The CPT code for slide preparation is the level of service (ie: 88305) with the TC modifier attached. That lets the insurance companies know that you prepared the slides for reading elsewhere. Should you have any questions or I may be of further assistance, please let me know. I may be reached at 1-877-822-7100, Kindest Regards, Louri Roberts Billing Consultants, LLC www.billingconsultants.net histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Sakura VIP-5 and Alarm System (William Chappell) 2. Re: mouse pituitary (tracy.bergeron@crl.com) 3. CPT Code for preparing on slide (Tom McNemar) 4. Re: embedding without a station? (Anila Syed) 5. Fat stains on P/S. (mtitford@aol.com) 6. HOSTOLOGIST Opportunities with BayCare Health System (Nevard, Jann) 7. RE: CPT Code for preparing on slide (Terri Braud) 8. RE: Ye Olde Days (Blazek, Linda) 9. RE: embedding without a station? (Blazek, Linda) 10. RE: RE: embedding without a station? (Ingles Claire) 11. Re: embedding without a station? (RSRICHMOND@aol.com) 12. RE: Ye Olde Days (Jackie M O'Connor) 13. Re: mouse pituitary (Roger Moretz) 14. RE: Ye Olde Days (Ford Royer) 15. Re: Re: embedding without a station? (Jennifer MacDonald) 16. histology position in Seattle (Ken Pierce) 17. Re: Goldner's Trichrome Staining (RSRICHMOND@aol.com) 18. Beta Gal staining on formalin fixed mouse embryo's (James Watson) 19. Tennessee Society for Histotechnology Call for abstracts (Hofecker, Jennifer L) 20. Re: Ye Olde Days (Joe Nocito) 21. Microtome Blades (MICHELLE SEAGLE) 22. Re: Re: Goldner's Trichrome Staining (Bryan Hewlett) 23. Bar Code Labels (Lena Spencer) 24. Robert Lott (judy webb) 25. RE: MICROTOME BLADES (Yvonne Jones) ---------------------------------------------------------------------- Message: 1 Date: Wed, 24 Jan 2007 10:04:50 -0800 (PST) From: William Chappell Subject: [Histonet] Sakura VIP-5 and Alarm System To: histonet@lists.utsouthwestern.edu Message-ID: <866756.76950.qm@web37212.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Greetings all, I have been assigned with the tast of finding a good after market alarm system (i.e. phone dialer, etc.) that pairs with our brand new Sakura VIP-5. Any ideas? Any Favorites? Any that just don't work right? Thanks, Will ------------------------------ Message: 2 Date: Wed, 24 Jan 2007 13:04:18 -0500 From: tracy.bergeron@crl.com Subject: Re: [Histonet] mouse pituitary To: histonet@pathology.swmed.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I did forget to mention we do this with Rat pituitary as well. Someone asked me about trimming. Once the skull is sufficiently decaled that it can be cut with a blade easily we trim away the excess skull, so that the only portion remaining is the xc containing the pituitary gland. Hope this is helpful. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 tracy.bergeron@crl.com Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 12:38 PM To histonet@pathology.swmed.edu cc Subject Re: [Histonet] mouse pituitary The best way to ensure you have pituitary present in your samples that come up from necropsy, is to have your necropsy folks open the skull up so formalin can get to the brain, but not remove the brain from the skull. You have a greater chance of keeping the pituitary intact if the brain is removed after fixation. Also for mouse pituitary we have found that because of it's size it can be very easily damaged if removed from the skull for processing. We generally remove the brain, then decalcify the skull with the pituitary in place (formic acid decal solution). Then process, embedd, and cut the xc of skull containing the pituitary. Since switching to this method we rarely have issues with lost or damaged pituitary tissue. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 Atoska Gentry Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2007 12:21 PM To Histonet cc Subject [Histonet] mouse pituitary Leon, thanks my apologies my wording was a little off. I should have said it's location in relation to mouse brain. Guess I got caught up with the inability to find it *in* the _Atlas of the Mouse Brain and Spinal Cord_ that we have on hand. I'm aware perfectly aware of it's general location. It's just that sometimes it's present in the samples rec'd from necropsy and other's it's not. And our research though primarily small animal does not routinely involve mice or rats. Thanks for your assistance. Atoska Leon Brokken wrote: The pituitary in both mouse and rats is located _under_ the brain, not _in_. It is rather easy to dissect however. If the skull is opened from the top, one can carefully lift the brain out of the skull after disconnecting the optical nerves. The pituitary will remain situated at the bottom of the brain 'cavity' and can then carefully be taken out as a whole. If you need more detailed help on dissection I can provide these (allthough it has been a while ago since I performed these dissections). Cheers, Leon. Atoska Gentry wrote: > Hello, does anyone have info on an atlas/manual in which the pituitary > of either mouse or rat brain is distinctively displayed? We have a > research collaborator who is specifically interested in studying > mouse pituitary. But, we have not been able to find an atlas which > shows it's exact location in mouse brain. And it is obviously not > distinguishable upon gross exam. Your prompt replies will be much > appreciated. Atoska :-) > > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 24 Jan 2007 13:05:12 -0500 From: "Tom McNemar" Subject: [Histonet] CPT Code for preparing on slide To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F44D@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" We may start doing some work for an outside office and I need to create a new billable test. We would be processing blocks, embedding, cutting, and staining for H&E. Could anyone share with me what CPT code you use or how you bill it? Thanks so much. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 4 Date: Wed, 24 Jan 2007 18:12:16 -0000 From: "Anila Syed" Subject: Re: [Histonet] embedding without a station? To: Message-ID: <009b01c73fe3$2f525740$42640a52@pbn> Content-Type: text/plain; charset="iso-8859-1" Wow!! Thanks for all the suggestions and trips down memory lane! I dont get how paper could have been used as moulds!! and I love the teapot idea!! As for the asbestos and lead etc, I won't mention any of that to our safety officer :) Thanks everyone, I will try to go with a beaker in the incubator and a hotplate, but will also try the doughnuts approach :) Thanks Anila ----- Original Message ----- From: "Monfils, Paul" To: Sent: Wednesday, January 24, 2007 4:50 PM Subject: RE: [Histonet] embedding without a station? We didn't have embedding stations when I started in histology. We had three stainless steel pitchers of melted paraffin, maybe 500 ml each, in the paraffin oven. We worked on a large, low temperature hotplate, called a "slide warmer". We would take one of the pitchers to the "work station", place it on the slide warmer. We would take the last paraffin container, containing the processed specimens, off the rotary processor and plug it in next to the hotplate. Take an embedding mold (these were made of paper, folded by the histotechs at the end of the previous workday), write the specimen ID on it, place it on the hotplate, pour paraffin into it from the pitcher, unwrap the tissue sample (which were processed wrapped in lens paper - no such thing as cassettes), place it in the mold, then transfer the mold to a metal plate on top of a cakepan full of ice. When the pitcher of paraffin started to get too cool, replace it in the oven and take another pitcher. Sounds pretty primitive - mainly because it was - but it got the job done. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anila Syed > Sent: Wednesday, January 24, 2007 5:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding without a station? > > Dear All, > > I have hundreds of carotid plaques to embedd. I have a tissue processor, but > no embedding station. Would anyone attempt to do this without an embedding > station or do you think I should go and try to find the facilities > somewhere? > > What did people do before embedding stations? > > Many thanks for your input and opinions, > > Anila Syed > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.410 / Virus Database: 268.17.8/649 - Release Date: 1/23/07 ------------------------------ Message: 5 Date: Wed, 24 Jan 2007 13:20:49 -0500 From: mtitford@aol.com Subject: [Histonet] Fat stains on P/S. To: histonet@lists.utsouthwestern.edu Message-ID: <8C90DFB81F67059-137C-11F5@WEBMAIL-MA20.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Tam Melville asks about fat stains on paraffin sections. Some lipids do remain in paraffin sections although most are removed by the organic solvents used in processing. I do not think any "fat droplets" would survive paraffin processing. Compound lipids, like sphingomyelin do survive and can be demonstrated by Sudan black, but the whole thing is less than perfect. They stain a kind of grey color. McManus published a method in which you take P/S sections to 70% alcohol, stain in sat. Sudan black in 70% alcohol for 30 min to 3 hours at 60oC, rinse in 70% alcohol, and then counterstain and mount in aquous mounting medium. I don't have the reference, but it is on page 302 of the 1968 edition of "Handbook of histopathological techniques" by C.F.A. Culling. We do it occasionally when a resident says "D***, I should have done a F/S fat stain on this" Michael Titford Pathology USA Mobile AL ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. ------------------------------ Message: 6 Date: Wed, 24 Jan 2007 14:09:31 -0500 From: "Nevard, Jann" Subject: [Histonet] HOSTOLOGIST Opportunities with BayCare Health System To: Message-ID: <98ECE5B11C59DE449F8949A45C52A8190400F95A@EXCHANGE02-VS.BCAD.BAYCARE.ORG> Content-Type: text/plain; charset="us-ascii" JOB POSTING FOR BAYCARE HEALTH SYSTEM TAMPA BAY AREA FLORIDA We are the 5th largest not-for-profit health care system in the U.S. and the largest on Florida's West Coast. Our nine area hospitals are leaders in research and teaching, and have been ranked among the nation's best health care providers. We can offer full time, part time or pool. Feel free to visit us on the web at www.baycarejobs.com. If you would like to hear more, please contact me below to set up a telephonic interview. The Histologist is responsible for all phases of tissue processing from fixation to final slide, including special stains and techniques; maintains all files, records, and documentation required by state and federal licensing boards; cleans and maintains equipment and instruments; stocks and maintains supplies and performs other duties as assigned. *Florida Licensure, 2+ years routine and special Histology experience. Work occasional weekend. Education: High school or equivalent Required RELATED FIELD AS Preferred Experience: One year LABORATORY Preferred Licensure: HISTOLOG Required ASCP Preferred CLS Preferred NCA Preferred Specific Skills Required: Computer skill appropriate to position Organizational skills Time management Work independently with minimal supervision Teamwork Interpersonal skills Medical terminology use and understanding Written and verbal communication skills Customer service skills Currently I have needs for day shift and night shift. We offer full benefits with matching 401K, generous relocation allowance, top 100 hospitals, great locations. Please call me at the numbers. Jann Nevard, CIR Regional Source Recruiter BayCare Health System Tel: 727-519-1315 Fax: 727-519-1323 Toll free: 1-866-221-3222 option 1 Web:www.baycarejobs.com e-mail: jann.nevard@baycare.org Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. ------------------------------ Message: 7 Date: Wed, 24 Jan 2007 14:18:49 -0500 From: "Terri Braud" Subject: RE: [Histonet] CPT Code for preparing on slide To: "Histonet \(E-mail\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Ahhhh.....the curse of the Histo billed tests. The CPT code for Processing, embedding, cutting and H&e's is based solely on the diagnosis of the type of tissue, per specimen, regardless of the number of blocks and slides produced. If you are doing this work to be read elsewhere, you might do better to come up with a contracted price, billed through separate invoices. Your financial folks should be able to add to capture your billed tests if you are worried about showing workload. If your pathologists are reading the slides, then you will have to bill based on diagnosis. You can arrange discounted pricing on current pricing through contracts from your hospital billing (if that's the route it takes) If I can help you negotiate through this, please just give me a call. Good Luck, T Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Wednesday, January 24, 2007 1:05 PM To: histonet@pathology.swmed.edu Subject: [Histonet] CPT Code for preparing on slide We may start doing some work for an outside office and I need to create a new billable test. We would be processing blocks, embedding, cutting, and staining for H&E. Could anyone share with me what CPT code you use or how you bill it? Thanks so much. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. ------------------------------ Message: 8 Date: Wed, 24 Jan 2007 14:20:30 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Ye Olde Days To: "Breeden, Sara" , Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684CDA@bruexchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Sally, Good grief you're old! But then of course I remember all of those things also so I guess we're not all that old if we can still remember them! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 24, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ye Olde Days Oh, goody! A Topic! I've been doing this histology thing since 1969 and BOY! have we come a long way! After a 20-year absence from a lab in Albuquerque, I returned to find all these KIDS doing histo - and MEN to boot! Okay, I'd been in the animal research world for a long time... (and Joyce, I worked in Hawaii in 1977 - we could swap tales of adventure!). So the KIDS asked several of us long-in-the-tooth techs how it was in The Old Days. We made up a story about how we had to keep chickens and bees in the back parking lot for the egg albumin (smearing on slides) and the wax. We almost had 'em going for a while there, because as Experienced Professionals, we could make up a really, really good tale! And I remember the "L" brackets, and the asbestos pad and the Lipshaw dispenser and those huge/tall "rings" where you laid the little piece of paper with the accession number. And, Fred, we all had ashtrays on the ledge above our work station. OMG! It's a wonder we're still alive! My old pathologist-boss still smokes and he's close to 90 - and he plays golf and is as sharp as ever. Aaaaahhhh... Ye Goode Olde Days! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 24 Jan 2007 14:23:12 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] embedding without a station? To: "Anila Syed" , Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684CDB@bruexchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Someone needs to teach Anila how to make a boat! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anila === message truncated === --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 25 05:26:29 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 25 05:26:39 2007 Subject: [Histonet] blades Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640987@hpes1.HealthPartners.int> I agree with the Dura-edge blades, high profile. We love them and the price is excellent!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Inga.Hansson <@t> neuro.uu.se Thu Jan 25 06:54:26 2007 From: Inga.Hansson <@t> neuro.uu.se (Inga Hansson) Date: Thu Jan 25 06:50:55 2007 Subject: [Histonet] testicles Message-ID: <5ba505dd415e96248c7cbe250d952cab@neuro.uu.se> Hi everyone, A collegue of mine is trying to section frozen rat testicle (perfused and cryoprotected in sucrose) at -30?C. Is that a good temperature to use? The sections seem fragile and tend to break. They?re 10 microns thick. Or is it bad fixation? Thanks in advance for any help! Inga From Susan.Walzer <@t> HCAHealthcare.com Thu Jan 25 07:38:48 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Thu Jan 25 07:38:28 2007 Subject: [Histonet] MICROTOME BLADES In-Reply-To: <45B76C2A.6040805@mclean.harvard.edu> Message-ID: <471953BC63077941B82C26A4338272B42F04E0@ORLEV03.hca.corpad.net> Accu-edge are the ONLY blades and we have over the years tried them all. No comparison. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tim Wheelock Sent: Wednesday, January 24, 2007 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MICROTOME BLADES Hi Everyone: I am trying other brands of disposable microtome blades. I use Surgipath high profile Teflon coated blades now. What would people recommend? Tim Wheelock Harvard Brain Bank McLean Hospital Belmont , MA 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jason.burrill <@t> crl.com Thu Jan 25 07:48:35 2007 From: jason.burrill <@t> crl.com (jason.burrill@crl.com) Date: Thu Jan 25 07:49:23 2007 Subject: [Histonet] re: Beta Gal staining on formalin fixed mouse embryo's Message-ID: I have used this protocol and reagents on formalin fixed tissue with good success. http://www.specialtymedia.com/05Resources/Protocols/Beta%20Gal%20in%20Tissue%20Protocol.htm Jason D. Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 ph: 978-658-6000 ext 1652 fax: 978-988-8793 E-mail: jason.burrill@crl.com From tkngflght <@t> yahoo.com Thu Jan 25 08:58:07 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Jan 25 08:59:42 2007 Subject: [Histonet] Histotemp positions--all shifts (and plenty of permanent jobs) In-Reply-To: Message-ID: <234909.50301.qm@web50913.mail.yahoo.com> Hi everyone! We're growing again and are seeking good techs interested in TRAVEL. I'm happy to spend time with you to answer all your questions if you're even just curious but not yet ready to go. ALL of our services are free and since I am a Histotech--it's first-hand from an experienced tech and traveler. We have all three shifts open in several locations, and we'll even cover half the license fee for anyone interested in working in New York City. We pay for everything and make the travel part easy. We have techs already working in these labs and they are well-managed happy environments--GREAT first assignments. Give me a call and I'll answer your questions--if you're not ready now you'll have more information to make a decision later!! And we have OODLES of permanent positions--it's nice to know what is out there. Current locations include: North Carolina Connecticut New York (we pay half the license fee) Washington DC Indiana Ohio Kansas California Florida Oklahoma Massechusetts Colorado South Carolina Texas And if there is somewhere you WANT to work, we'll find the job for you! Thank you! Cheryl 800.756.3309 Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From YuJ2 <@t> upmc.edu Thu Jan 25 09:06:26 2007 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Thu Jan 25 09:08:14 2007 Subject: [Histonet] A question on bone sections In-Reply-To: <471953BC63077941B82C26A4338272B42F04E0@ORLEV03.hca.corpad.net> Message-ID: <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> Dear all, We have parrafin embedded bone of mouse tibia (decalcified). Can sections (longitudinal) of the bone be cut with a regular microtome blade or a special blade? We intend to do H&E staining on these sections and cut soft tissue sections (mouse) in our own lab routinely. This is the first time we are dealing with bone. Any advice will be very helpful and appreciated. Thanks, Jian ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Phone: 412-623-7786 Email: yuj2@upmc.edu ******************************************************************* From YuJ2 <@t> upmc.edu Thu Jan 25 09:12:25 2007 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Thu Jan 25 09:14:28 2007 Subject: [Histonet] A question on bone sections In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> Message-ID: <7E0A77BFEB9A1E47A63F978E7116821F02AEC668@1upmc-msx11.acct.upmchs.net> Sorry that I forgot to mention that we are interested in looking at bone marrow before and after whole body irradiation. Thank you. Jian -----Original Message----- From: Yu, Jian Sent: Thursday, January 25, 2007 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: A question on bone sections Dear all, We have parrafin embedded bone of mouse tibia (decalcified). Can sections (longitudinal) of the bone be cut with a regular microtome blade or a special blade? We intend to do H&E staining on these sections and cut soft tissue sections (mouse) in our own lab routinely. This is the first time we are dealing with bone. Any advice will be very helpful and appreciated. Thanks, Jian ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Phone: 412-623-7786 Email: yuj2@upmc.edu ******************************************************************* From Histology <@t> acute.sney.nhs.uk Thu Jan 25 09:20:50 2007 From: Histology <@t> acute.sney.nhs.uk (Histology (Path Lab)) Date: Thu Jan 25 09:20:03 2007 Subject: [Histonet] procedure to xray fixed breast tissue Message-ID: <4EAF6D4C18D9384492E6395AF94D81CB06AF8B4A@liam> Hi I would be grateful for any information to help us set up a procedure to x-ray breast tissue here at Scarborough hospital. Any ideas on what is the best type of board to fix the slices of breast to and to cover them with to keep them in the same position to enable the orientation to be maintained whilst being x-rayed, would be greatly appreciated. Thanks Cita Ball Scarborough histology department From mcauliff <@t> umdnj.edu Thu Jan 25 09:05:30 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jan 25 09:24:54 2007 Subject: [Histonet] Malt diastase In-Reply-To: <38E5B431A5228D46A9A99E0206D5788317CEA5@medwin.ad.stir.ac.uk> References: <38E5B431A5228D46A9A99E0206D5788317CEA5@medwin.ad.stir.ac.uk> Message-ID: <45B8C73A.5010102@umdnj.edu> I use Sigma porcine pancreatic amylase, 0.25% in 4mM sodium acetate buffer pH 6.0, for 10-15 minutes at room temp. This works well on liver fixed in buffered formalin. Geoff Deborah Faichney wrote: >Dear all, > >I need to obtain malt diastase for Periodic acid schiff staining for >glycogen in blood smears. I am finding this difficult (in the UK) and >would like to know from any carbohydate people out there what I could >use instead. > >Thanks > >Debbie Faichney >Institute of Aquaculture >Stirling, Scotland > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From joel.thompson <@t> uky.edu Thu Jan 25 09:35:45 2007 From: joel.thompson <@t> uky.edu (Joel Thompson) Date: Thu Jan 25 09:37:42 2007 Subject: [Histonet] disposable knives Message-ID: <9570ccf10701250735x11db649bvfb6119774d5be46@mail.gmail.com> i'm using a microm 315 with disposable knives. how many sections should i expect to get from each knife cutting kidney in paraffin? thanks -- Joel Thompson Research Analyst Cardiovascular Research Center Gill Heart Institute University of Kentucky From PMonfils <@t> Lifespan.org Thu Jan 25 09:49:18 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jan 25 09:53:22 2007 Subject: [Histonet] testicles In-Reply-To: <5ba505dd415e96248c7cbe250d952cab@neuro.uu.se> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C05@LSRIEXCH1.lsmaster.lifespan.org> -30 is too cold to section testicles. I cut them at -15 C. Sometimes even -12 C. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Inga Hansson > Sent: Thursday, January 25, 2007 4:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] testicles > > Hi everyone, > > A collegue of mine is trying to section frozen rat testicle (perfused > and cryoprotected in sucrose) at -30?C. Is that a good temperature to > use? The sections seem fragile and tend to break. They?re 10 microns > thick. Or is it bad fixation? > > Thanks in advance for any help! > > Inga > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From abright <@t> brightinstruments.com Thu Jan 25 10:15:53 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Jan 25 10:16:31 2007 Subject: [Histonet] testicles Message-ID: Dear Inga, On our cryostats I would have the specimen temperature set @ -12?C and the microtome chamber temperature set @ -20?C as this will stop any adherence to the knife and anti-roll plate. You are much too cold at present on the specimen temperature. I see you are a neuro lab these settings would be fine for brain too. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Inga Hansson [mailto:Inga.Hansson@neuro.uu.se] Sent: 25 January 2007 12:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] testicles Hi everyone, A collegue of mine is trying to section frozen rat testicle (perfused and cryoprotected in sucrose) at -30?C. Is that a good temperature to use? The sections seem fragile and tend to break. They?re 10 microns thick. Or is it bad fixation? Thanks in advance for any help! Inga _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Rettman <@t> us.crl.com Thu Jan 25 10:08:31 2007 From: Kathy.Rettman <@t> us.crl.com (Rettman, Kathy A.) Date: Thu Jan 25 11:14:23 2007 Subject: [Histonet] FW: confirm db9b48815ee9266a68edd0803028684deecfea6d Message-ID: <79DC2FFD71913342BB04ED8BBC591F24EE6946@wor-crl-exch1.na01.crl.com> Please unsubscribe me. Thank you, Kathy A. Rettman, HT (ASCP) Laboratory Manager Charles River Laboratories, Preclinical Services 6217 Centre Park Drive West Chester, Ohio 45069 513-779-9600 x228 Fax: 513-779-9603 e-mail: kathy.rettman@us.crl.com -----Original Message----- From: Rettman, Kathy A. Sent: Friday, December 15, 2006 12:15 PM To: 'histonet-request@lists.utsouthwestern.edu' Subject: RE: confirm db9b48815ee9266a68edd0803028684deecfea6d Yes, I would like to be on the list. Thank you, Kathy A. Rettman, HT (ASCP) Laboratory Manager Charles River Laboratories, Preclinical Services 6217 Centre Park Drive West Chester, Ohio 45069 513-779-9600 x228 Fax: 513-779-9603 e-mail: kathy.rettman@us.crl.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, December 15, 2006 8:12 AM To: Rettman, Kathy A. Subject: confirm db9b48815ee9266a68edd0803028684deecfea6d Mailing list subscription confirmation notice for mailing list Histonet We have received a request from 207.247.42.130 for subscription of your email address, "kathy.rettman@us.crl.com", to the histonet@lists.utsouthwestern.edu mailing list. To confirm that you want to be added to this mailing list, simply reply to this message, keeping the Subject: header intact. Or visit this web page: http://lists.utsouthwestern.edu/mailman/confirm/histonet/db9b48815ee9266 a68edd0803028684deecfea6d Or include the following line -- and only the following line -- in a message to histonet-request@lists.utsouthwestern.edu: confirm db9b48815ee9266a68edd0803028684deecfea6d Note that simply sending a `reply' to this message should work from most mail readers, since that usually leaves the Subject: line in the right form (additional "Re:" text in the Subject: is okay). If you do not wish to be subscribed to this list, please simply disregard this message. If you think you are being maliciously subscribed to the list, or have any other questions, send them to histonet-owner@lists.utsouthwestern.edu. From Jessica.Vacca <@t> HCAhealthcare.com Thu Jan 25 12:05:08 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Thu Jan 25 12:06:41 2007 Subject: [Histonet] H.Pylori Message-ID: <41E16A15CE78374EA45B57E0F94339B801F8173F@ORLEV01.hca.corpad.net> Does anyone that works in a Hospital have their Micro department grow H. pylori and create their own controls by injecting into the tissue? If you do, do you mind getting me their procedure for this? Thanks in advance for your help. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From MDiCarlo <@t> KaleidaHealth.Org Thu Jan 25 09:50:06 2007 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Jan 25 12:10:42 2007 Subject: [Histonet] A question on bone sections In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> References: <471953BC63077941B82C26A4338272B42F04E0@ORLEV03.hca.corpad.net> <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> Message-ID: <9B4A77DF11463E4FB723D484214AE9BC02517395@KALEXMB02.KaleidaHealth.org> Jian, Yes, you can cut the decaled mice tibia with a disposable or regular microtome blade. Before cutting, I soak a few of the blocks in decal, in an empty slide box. Set it on top of a block of ice and add some water, soak for about 10 minutes, rinse block under cold water and then it is ready to be cut. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yu, Jian Sent: Thursday, January 25, 2007 10:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question on bone sections Dear all, We have parrafin embedded bone of mouse tibia (decalcified). Can sections (longitudinal) of the bone be cut with a regular microtome blade or a special blade? We intend to do H&E staining on these sections and cut soft tissue sections (mouse) in our own lab routinely. This is the first time we are dealing with bone. Any advice will be very helpful and appreciated. Thanks, Jian ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Phone: 412-623-7786 Email: yuj2@upmc.edu ******************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From PMonfils <@t> Lifespan.org Thu Jan 25 10:02:38 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jan 25 12:16:20 2007 Subject: [Histonet] A question on bone sections In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C06@LSRIEXCH1.lsmaster.lifespan.org> If by a "regular microtome blade" you mean a standard thickness disposable blade, then you will probably have difficulties. Standard disposable blades are too thin to cut very dense tissues without flexing. I do a lot of bone work here , on mice and larger animals. Using a standard disposable blade on bone will often result in chatter or "venetian blind" effect, and sometimes chipping out pieces from the block. For bone I use Surgipath thick blades. They twice as thick as standard blades and much more rigid. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Yu, Jian > Sent: Thursday, January 25, 2007 7:06 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] A question on bone sections > > Dear all, > > We have parrafin embedded bone of mouse tibia (decalcified). Can > sections (longitudinal) of the bone be cut with a regular microtome > blade or a special blade? We intend to do H&E staining on these sections > and cut soft tissue sections (mouse) in our own lab routinely. > > This is the first time we are dealing with bone. Any advice will be > very helpful and appreciated. > > Thanks, Jian > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Phone: 412-623-7786 > Email: yuj2@upmc.edu > ******************************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From FUNKM <@t> mercyhealth.com Thu Jan 25 12:30:31 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jan 25 12:33:06 2007 Subject: [Histonet] (no subject) Message-ID: Looking at purchasing a new tissue processor. Any help will be great. We have had Sakura and hahave not been as happy with the door in front that keeps falling open. Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 From nancy.troiano <@t> yale.edu Thu Jan 25 12:41:34 2007 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Thu Jan 25 12:43:29 2007 Subject: [Histonet] bone sections Message-ID: <5.2.1.1.2.20070125133953.00bcc030@email.med.yale.edu> I would suggest using high profile disposable blades for decalcified bone specimens. They can be cut on a regular paraffin microtome (not motorized). We usually trim the block almost to the area of interest, then place it on crushed ice to chill before cutting. From FUNKM <@t> mercyhealth.com Thu Jan 25 12:38:13 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jan 25 12:52:03 2007 Subject: [Histonet] Looking at auto coverslipper do you have any suggestions ? Message-ID: Looking at auto coverslipper do you have any suggestions ? thanks Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 From Jessica <@t> medstaffservices.com Thu Jan 25 12:47:39 2007 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Thu Jan 25 13:13:44 2007 Subject: [Histonet] CURRENT EMPLOYMENT OPPROTUNITIES Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: ~$30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k 3. Generalist Hours: Monday - Friday, Day Shift Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From rjbuesa <@t> yahoo.com Thu Jan 25 13:22:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 25 13:23:54 2007 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <11555.78393.qm@web61213.mail.yahoo.com> A well cleaned and adjusted door does not fall open easily. Ren? J. Marcia Funk wrote: Looking at purchasing a new tissue processor. Any help will be great. We have had Sakura and hahave not been as happy with the door in front that keeps falling open. Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From KPierce <@t> cancer-test.com Thu Jan 25 13:06:34 2007 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Thu Jan 25 13:42:43 2007 Subject: [Histonet] Histo position in Seattle Message-ID: <9380B79A09A6DD43884D977256FC12020B0640@fleming.mla.local> On Jan. 24, a possible position in Seattle was put on the Histonet. As of today that job will not be availbale. Ken Pierce, Histo Supervisor. From RSRICHMOND <@t> aol.com Thu Jan 25 14:01:23 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Jan 25 14:10:37 2007 Subject: [Histonet] Re: procedure to xray fixed breast tissue Message-ID: Cita Ball asks: I would be grateful for any information to help us set up a procedure to x-ray [fixed] breast tissue here at Scarborough hospital [in the UK]. Any ideas on what is the best type of board to fix the slices of breast to and to cover them with to keep them in the same position to enable the orientation to be maintained whilst being x-rayed, would be greatly appreciated. A surgical pathologists needs to be prepared to X-ray unfixed and fixed wet tissue, as well as paraffin blocks. Usually it's best to lay the tissue down on a piece of plastic (such as a plastic tray) and take the tray to the radiology department and get a specimen radiogram done on it. It's helpful to phone ahead, and if you haven't done it before, you should talk to a radiologist about it. It's a good thing to fix the tissue as flat as possible - on a piece of cardboard - if you anticipate getting specimen radiography done after fixation. A lot of such procedures can be avoided if the radiographers can be persuaded to send the original specimen radiogram along with the wire localization specimen. For convenience they can shoot two films (since there is no radiation exposure to the patient) so that the pathology service can discard the film rather than take the trouble to return and re-file it. You need to have a view box - holding the film up to a fluorescent light and squinting isn't satisfactory. If you can't have a view box (the red-haired stepchild again) go to a photography supply store and buy one of the light units photographers use for looking at negatives and cutting film. Scarborough - is that where they cook chicken with parsley, sage, rosemary, and thyme... the renowned Scarborough Fowl... don't throw that view box at me... Bob Richmond Samurai Pathologist Knoxville TN From hej01 <@t> health.state.ny.us Thu Jan 25 14:34:14 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Jan 25 14:43:39 2007 Subject: [Histonet] nitrile gloves Message-ID: Hi Histonetters, Does anyone know if there is a vendor that sells 1/2 size nitrile gloves? The small size is too tight and the medium is too big to do proper coverslipping. Helen Johnson (hej01@health.state.ny.us) From BoozerKA <@t> ah.org Thu Jan 25 14:45:04 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Thu Jan 25 14:47:31 2007 Subject: [Histonet] Re: procedure to xray fixed breast tissue In-Reply-To: References: Message-ID: <45B8A650020000C000002683@ahgwrsvl.ah.org> We use large culture dishes from Micro and put the smaller of the two on top of the larger to hold the tissue flat. I went to the Dollar Store and bought tortilla warmers and put Biohazard stickers on them to use for transporting the culture dishes back and forth from Radiology. The fluids are kept intact and the specimen is covered during transport. >>> 01/25/07 12:01 PM >>> Cita Ball asks: I would be grateful for any information to help us set up a procedure to x-ray [fixed] breast tissue here at Scarborough hospital [in the UK]. Any ideas on what is the best type of board to fix the slices of breast to and to cover them with to keep them in the same position to enable the orientation to be maintained whilst being x-rayed, would be greatly appreciated. A surgical pathologists needs to be prepared to X-ray unfixed and fixed wet tissue, as well as paraffin blocks. Usually it's best to lay the tissue down on a piece of plastic (such as a plastic tray) and take the tray to the radiology department and get a specimen radiogram done on it. It's helpful to phone ahead, and if you haven't done it before, you should talk to a radiologist about it. It's a good thing to fix the tissue as flat as possible - on a piece of cardboard - if you anticipate getting specimen radiography done after fixation. A lot of such procedures can be avoided if the radiographers can be persuaded to send the original specimen radiogram along with the wire localization specimen. For convenience they can shoot two films (since there is no radiation exposure to the patient) so that the pathology service can discard the film rather than take the trouble to return and re-file it. You need to have a view box - holding the film up to a fluorescent light and squinting isn't satisfactory. If you can't have a view box (the red-haired stepchild again) go to a photography supply store and buy one of the light units photographers use for looking at negatives and cutting film. Scarborough - is that where they cook chicken with parsley, sage, rosemary, and thyme... the renowned Scarborough Fowl... don't throw that view box at me... Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Thu Jan 25 14:54:01 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Thu Jan 25 14:55:33 2007 Subject: [Histonet] Used equipment Message-ID: We have a 6-7 yr. old Mopec grossing station we need to get rid of. According to depreciation from powers that be the unit is still worth a substantial amount. Equipment like that is only worth what someone will give you for it of course. Does anyone out there have any idea who might want this unit or who buys this type of used equipment. Thank you in advance... Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Heather.D.Renko <@t> osfhealthcare.org Thu Jan 25 14:24:41 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Jan 25 15:01:14 2007 Subject: [Histonet] Job Opening Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775302@pmc-rfd-mx01.intranet.osfnet.org> Histotech I position open in Rockford, Illinois. Must be certified HT(ASCP) Full time with benefits and generous PTO. www.osfhealthcare.org Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 heather.renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From PMonfils <@t> Lifespan.org Thu Jan 25 12:48:31 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jan 25 15:01:32 2007 Subject: [Histonet] H.Pylori In-Reply-To: <41E16A15CE78374EA45B57E0F94339B801F8173F@ORLEV01.hca.corpad.net> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C09@LSRIEXCH1.lsmaster.lifespan.org> I haven't done this with H. pylori, but I have done it with other organisms, including gram+ and gram- bacteria, acid-fast organisms, and yeast as a fungus control. I prefer lung as the tissue because there are so many natural spaces to receive the organisms. If you inject into something like liver, the tissue will have to separate to receive the injection, and there will be little to hold the injected culture in place. My preferred method is to take a block of fresh tissue, push the needle almost all the way through, then inject as I withdraw the needle. Do this three or four times, then leave the tissue in a covered petrie dish with a moist piece of paper towel to maintain humidity, at room temperature for a couple of hours. This allows the organisms to settle and adhere to the tissue. Then just drop the tissue into formalin, let it fix, and process as usual. I have also injected into pre-fixed lung and gotten usable results, but not as good as with the above method. In this case there is no need to wait a couple of hours because the organisms will die as soon as they are injected, so just inject and drop into formalin. For gram+ controls I use a mix of Bacillus subtilis and Staphylococcus epidermidis. For gram- a mix of Escherichia coli and Micrococcus luteus. I purchase the cultures from a biological supply company - connval@ctvalleybio.com - for less than $10 each, in liquid medium, not on plates. I spin them down to concentrate the organisms, pour off most of the medium, resuspend by vortexing, mix the cultures if I want a mix, then draw into a syringe and inject as above. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Vacca Jessica > Sent: Thursday, January 25, 2007 10:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H.Pylori > > Does anyone that works in a Hospital have their Micro department grow H. > pylori and create their own controls by injecting into the tissue? If > you do, do you mind getting me their procedure for this? Thanks in > advance for your help. > > Jessica Vacca > Histology Supervisor > Brandon Regional Hospital > 119 Oakfield Dr. > Brandon Fl 33511 > (813) 571-5193 or (813) 681-5551 ext 2454 > Jessica.Vacca@hcahealthcare.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From hkromer <@t> KaiserAssociates.com Thu Jan 25 16:03:00 2007 From: hkromer <@t> KaiserAssociates.com (Heather Kromer) Date: Thu Jan 25 15:57:37 2007 Subject: [Histonet] Easy way to make $50-150 Message-ID: Dear Histonetters, Our firm, Kaiser Associates, is conducting research on Advanced Staining processes, and would like your input. By participating in a survey you will be influencing potential upcoming changes to the market for Advanced Staining products. The completion of this survey should take only 25 minutes, and in return for your participation you will receive an honorarium of US $50 -150.00, depending on your qualifications. If you would like to participate in this survey please reply to Hkromer@kaiserassociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name We will send then you the weblink to the survey. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research based international consulting firm, dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com From kgibbon <@t> qltinc.com Thu Jan 25 16:18:49 2007 From: kgibbon <@t> qltinc.com (Kevin Gibbon) Date: Thu Jan 25 16:19:37 2007 Subject: [SPAM] - [Histonet] nitrile gloves - Email has different SMTP TO: and MIME TO: fields in the email addresses Message-ID: <66A24278845C594C98E5ECB840D7BFB503D9E903@VAN-EXCH-VS1.qltinc.com> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Thursday, January 25, 2007 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM] - [Histonet] nitrile gloves - Email has different SMTP TO: and MIME TO: fields in the email addresses Hi Histonetters, Does anyone know if there is a vendor that sells 1/2 size nitrile gloves? The small size is too tight and the medium is too big to do proper coverslipping. Helen Johnson (hej01@health.state.ny.us) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 25 13:02:34 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 25 17:04:01 2007 Subject: [Histonet] Breast tissues Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640990@hpes1.HealthPartners.int> With the new CAP standards regarding formalin fixation, I have another concern brought to me by our PA's. They are having to basically gross all breast specimens in fresh or barely fixed and are having a hard time getting the proper thickness (or should I say "thinness") due to minimal stabilization of the tissue from not being in a fixative long enough. The interpretation of the standards is that the tumor should be promptly examined grossly and placed in formlain after appropriate sectioning. Does anyone have any suggestions for the PA's to get sections that are the proper thickness with such fresh tissue? We would appreciate any help in this area!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From denise.woodward <@t> uconn.edu Thu Jan 25 11:06:03 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Jan 25 17:11:59 2007 Subject: [Histonet] pens References: <0E394B648E5284478A6CCB78E5AFDA270364097C@hpes1.HealthPartners.int> Message-ID: You can try to "refresh" dried pens by pouring a very thin level of acetone in a beaker and placing the pens, tip down, into the beaker. Cover and leave overnight. The level of the acetone should be no higher than the pen tips. Sometimes it works, sometimes not. Good luck! Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Sciences Storrs, Connecticut -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, January 24, 2007 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] pens We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 25 13:23:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 25 17:24:43 2007 Subject: [Histonet] Looking at auto coverslipper do you have any suggestions ? In-Reply-To: Message-ID: <216449.56935.qm@web61212.mail.yahoo.com> Try the film coverslipper by Sakura. Ren? J. Marcia Funk wrote: Looking at auto coverslipper do you have any suggestions ? thanks Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From cgfields <@t> lexhealth.org Thu Jan 25 14:27:40 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Thu Jan 25 17:29:10 2007 Subject: [Histonet] Used equipment Message-ID: We have a 6-7 yr. old Mopec grossing station we need to get rid of. According to depreciation from powers that be the unit is still worth a substantial amount. Equipment like that is only worth what someone will give you for it of course. Does anyone out there have any idea who might want this unit or who buys this type of used equipment. Thank you in advance... Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From ander093 <@t> tc.umn.edu Thu Jan 25 14:16:52 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Thu Jan 25 17:36:18 2007 Subject: [Histonet] disposable knives In-Reply-To: <9570ccf10701250735x11db649bvfb6119774d5be46@mail.gmail.com > References: <9570ccf10701250735x11db649bvfb6119774d5be46@mail.gmail.com> Message-ID: <6.2.3.4.0.20070125141428.04340850@ander093.email.umn.edu> I prefer the DuraEdge blades as well. They seem to much better than the AccuEdge do. Hence, less frequent changing of blades. I do believe that the type of blade holder plays a part in which blades work well on any given microtome though. LuAnn >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkarlisch <@t> psu.edu Thu Jan 25 17:10:55 2007 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Thu Jan 25 17:43:01 2007 Subject: [Histonet] Grossing Stations Message-ID: <20070125T181055Z_D9B900000000@psu.edu> All, We are considering purchasing two large grossing stations with some extra options. Can any of you recommend the grossing stations that you would prefer. We would like the grossing station to last for a reasonable amount of time, be sturdy in construction, offer options that can be added later but also be user friendly with enough shelf space and work area. Most importantly the ventilation must be of high quality. We have several companies that we are looking at but would like to get an idea of what most folks are using and if you are happy with the units or not. If you could also tell us what features you liked. Thank you in advance, Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From lpwenk <@t> sbcglobal.net Thu Jan 25 17:40:24 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jan 25 17:44:09 2007 Subject: [Histonet] H.Pylori In-Reply-To: <41E16A15CE78374EA45B57E0F94339B801F8173F@ORLEV01.hca.corpad.net> Message-ID: <000b01c740da$30f43f30$5b0d2e4b@HPPav2> I talked with someone in our Microbiology department about this a year or two ago. Basically, the answer is - H. pylori cannot be grown in a routine hospital microbiology lab. Think of the conditions under which it grows in the stomach - acidic environment, mucin around it, very low oxygen, plus it is giving off lots of unusual compounds, such as urease. Routine labs are not set up for this. As a result, Hp are VERY difficult and trick to grow/culture. If given the right environment (extremely difficult conditions), Hp will tend to survive but do not reproduce. The colonies go not get bigger. So, infortunately, histology and microbiology cannot collaborate to make Hp controls blocks like we can make other bacteria control blocks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Thursday, January 25, 2007 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H.Pylori Does anyone that works in a Hospital have their Micro department grow H. pylori and create their own controls by injecting into the tissue? If you do, do you mind getting me their procedure for this? Thanks in advance for your help. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Thu Jan 25 17:44:41 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Jan 25 17:48:29 2007 Subject: [SPAM] - [Histonet] nitrile gloves - Email has different SMTP TO: and MIME TO: fields in the email addresses In-Reply-To: <66A24278845C594C98E5ECB840D7BFB503D9E903@VAN-EXCH-VS1.qlti nc.com> Message-ID: <4.3.2.7.2.20070125163919.02bc87b0@algranth.inbox.email.arizona.edu> We have found there to be big differences in sizes from glove brand to glove brand. Try other brands in both sizes and see if one or another works better for you. I just called my reps or saw them at vendor shows and they sent a few sample gloves for us to try out. In this lab we have a problem getting gloves small enough and not every company has XS size. We finally agreed on MicroFlex UltraSense powder free nitrile gloves from VWR. They are nice and thin for gripping slides and coverslips. Andi At 02:18 PM 1/25/2007 -0800, Kevin Gibbon wrote: > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E >Johnson >Sent: Thursday, January 25, 2007 12:34 PM >To: histonet@lists.utsouthwestern.edu >Subject: [SPAM] - [Histonet] nitrile gloves - Email has different SMTP >TO: and MIME TO: fields in the email addresses > > >Hi Histonetters, > Does anyone know if there is a vendor that sells 1/2 size nitrile >gloves? The small size is too tight and the medium is too big to do >proper coverslipping. > >Helen >Johnson (hej01@health.state.ny.us) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This electronic transmission (including any and all attachments) is >intended solely for the use of the individual or entity to whom it is >addressed and may contain information that is privileged and/or >confidential. If you are not the intended recipient of this electronic >transmission, you are hereby notified that any disclosure, copying or >distribution, or the taking of any action in reliance upon the contents of >this electronic transmission, is strictly prohibited, and you are further >requested to purge this electronic transmission and all copies thereof >from your computer system. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From lpaveli1 <@t> hurleymc.com Thu Jan 25 19:22:30 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Jan 25 19:23:07 2007 Subject: [Histonet] Grossing Stations Message-ID: <45B91187020000EE00010BBE@smtp-gw.hurleymc.com> Pat, We just finished a lab renovation and have installed a Mopec 600, much to our pathology assistant's pleasure. The company was great to work with. Was willing to make any personal alterations and works like a dream. The ventilation (which had always been an issue w/our old system) is now totally solved. Had a air monitoring consultating company in yesterday, and it passed with flying colors. Tell 'em, "Morguebob sent me"!!! (joke) Lynette >>> "Patricia Karlisch" 01/25/07 6:10 PM >>> All, We are considering purchasing two large grossing stations with some extra options. Can any of you recommend the grossing stations that you would prefer. We would like the grossing station to last for a reasonable amount of time, be sturdy in construction, offer options that can be added later but also be user friendly with enough shelf space and work area. Most importantly the ventilation must be of high quality. We have several companies that we are looking at but would like to get an idea of what most folks are using and if you are happy with the units or not. If you could also tell us what features you liked. Thank you in advance, Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From d.fuster <@t> ub.edu Fri Jan 26 01:24:26 2007 From: d.fuster <@t> ub.edu (Dolors) Date: Fri Jan 26 01:24:48 2007 Subject: [Histonet] Re: embedding without a station? Message-ID: <45B9ACAA.8000807@ub.edu> uupss...the old microtome is leitz not leica.....the leica is too modern for us :-) From d.a.faichney <@t> stir.ac.uk Fri Jan 26 04:00:35 2007 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Fri Jan 26 04:00:54 2007 Subject: [Histonet] malt diastase replies Message-ID: <38E5B431A5228D46A9A99E0206D5788317CEAA@medwin.ad.stir.ac.uk> Thanks to all. I should have said that I would spit on my own slides, but this is for a lab of MSc students and the course director doesn't think 20 students spitting on their slides is good idea!!! Although if I can't order alternatives then they might have no choice!! (Geoff, thanks for the porcine amylase suggestion. I'll see if I can order some) Debbie -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. From BMolinari <@t> heart.thi.tmc.edu Fri Jan 26 05:23:55 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Jan 26 05:25:00 2007 Subject: [Histonet] A question on bone sections In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> Message-ID: As long as the bone is decalcified properly you should have no problem cutting the tibia on a regular microtome with a disposable blade. I cut mouse tibia this way and have no problems. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yu, Jian Sent: Thursday, January 25, 2007 9:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question on bone sections Dear all, We have parrafin embedded bone of mouse tibia (decalcified). Can sections (longitudinal) of the bone be cut with a regular microtome blade or a special blade? We intend to do H&E staining on these sections and cut soft tissue sections (mouse) in our own lab routinely. This is the first time we are dealing with bone. Any advice will be very helpful and appreciated. Thanks, Jian ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Phone: 412-623-7786 Email: yuj2@upmc.edu ******************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Jan 26 06:46:30 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jan 26 06:46:40 2007 Subject: [Histonet] Looking at auto coverslipper do you have anysuggestions ? References: <216449.56935.qm@web61212.mail.yahoo.com> Message-ID: We have to have a glass coverslipper and we really love our Leica CV5030. It is very easy to use and works great. And if down the road you need a new H&E stainer, the coverslipper can be attached to it via a transport station and then it's hands-free once you load the slides to stain until they come off coverslipped. Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 1/25/2007 2:23 PM To: Marcia Funk; histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] Looking at auto coverslipper do you have anysuggestions ? Try the film coverslipper by Sakura. Ren? J. Marcia Funk wrote: Looking at auto coverslipper do you have any suggestions ? thanks Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Fri Jan 26 08:34:32 2007 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Jan 26 08:38:39 2007 Subject: [Histonet] Histology Position Opening at Minneapolis VA Message-ID: Dear Histonetters, We have an opening in our Histology Dept. Our lab is staffed by 6 genuinely nice folks and we're looking for a 7th . We staff the lab Monday thru Friday (NO WEEKENDS!) and get 8 paid holidays per year, not to mention terrific benefits and competitive wages. This is a teaching hospital and we have many opportunities to learn, as well as teach. Please see www.usajobs.gov. The posting is either there now or will be soon. . The closing date for applying happens QUICKly after it's posted so if you need some guidance on the application process, feel free to call me at 612-467-2467. Sandy Harrison, Supervisor Anatomic and Surgical Pathology From TownsendD <@t> childrensdayton.org Fri Jan 26 09:23:00 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Jan 26 09:27:16 2007 Subject: [Histonet] pens Message-ID: Xylene also works the same way, with the pens soaking in for an hour or so. Or you can take the back off the pen and add 3-4 drops of xylene, store the pen tip down for a couple of minutes to let it soak through, and it works again. Dolores >>> "Woodward, Denise" 1/25/2007 12:06 PM >>> You can try to "refresh" dried pens by pouring a very thin level of acetone in a beaker and placing the pens, tip down, into the beaker. Cover and leave overnight. The level of the acetone should be no higher than the pen tips. Sometimes it works, sometimes not. Good luck! Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Sciences Storrs, Connecticut -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, January 24, 2007 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] pens We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian.chelack <@t> usask.ca Fri Jan 26 09:24:56 2007 From: brian.chelack <@t> usask.ca (Brian Chelack) Date: Fri Jan 26 11:49:30 2007 Subject: [Histonet] H pylori controls Message-ID: <000501c7415e$22b13ac0$0f13e980@PDS04> Hello Jessica; Funny you should mention the H pylori controls. I have been working this week with one of our graduate students to create some new formalin fixed control blocks. What we have done is grown up some ATCC 26695 H. pylori and mixed it with some low melting point agarose, created small plugs by adding the still melted agarose into 96 well PVC plates, removing the cooled plugs and drop them into formalin and process into paraffin blocks. They are quite nice. I told the grad student that people would probably pay for controls as nice as these so now she has gone and made hundreds of them. I figure that for all her time and initiative that a block like this should be worth $20. If you or any one else is interested, I can give you her email address and you can negotiate. I know that I have enough control material to last for the next 50 years. Brian Chelack Prairie Diagnostic Services 52 Campus Drive Saskatoon, SK S7N 5B4 306-966-7211 Confidentiality Notice: The information contained in this message and/or attachment may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. From Heather.D.Renko <@t> osfhealthcare.org Fri Jan 26 11:24:52 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Jan 26 12:00:23 2007 Subject: [Histonet] coverslipper Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775307@pmc-rfd-mx01.intranet.osfnet.org> Sakura tape/film Coverslipper works very well for us and rarely do we have problems with it, maybe once in the past year and it was something we had failed to do not the instrument. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From KMENDELL <@t> nhs-healthlink.org Fri Jan 26 12:02:26 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Fri Jan 26 12:02:47 2007 Subject: [Histonet] Hope someone out there can let me know what you do. I was having an issue of numbers coming off the Message-ID: <45B9FBE2020000A80001008A@mail.NHS-HEALTHLINK.ORG> Hope someone out there can let me know what you do. I was having an issue of numbers coming off the cassettes during processing. After trying different thing for the last two months the PA informed me that Carnoys was being used (to set the ink) and was not always rinsed off. This may also explain the problem I was having with my recycler (formalin). The PA switch to 4% acetic acid, blocks are better (a little) but she said she still isn't rinsing and there is no need to that others out there are not rinsing. What do others do. Kate ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From jcline <@t> wchsys.org Fri Jan 26 12:35:34 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Jan 26 12:35:48 2007 Subject: [Histonet] Sakura VIP-5 and Alarm System In-Reply-To: <866756.76950.qm@web37212.mail.mud.yahoo.com> Message-ID: <002201c74178$c4f303d0$1d2a14ac@wchsys.org> I have a Sensaphone 1104 by Phonetics,Inc. The Sensaphone calls 3 phone programmed numbers in succession until someone answers the call. I have it programmed to call three different people at home since we do not work evenings. ****************************************************************** Sent: Wednesday, January 24, 2007 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura VIP-5 and Alarm System Greetings all, I have been assigned with the tast of finding a good after market alarm system (i.e. phone dialer, etc.) that pairs with our brand new Sakura VIP-5. Any ideas? Any Favorites? Any that just don't work right? Thanks, Will _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From HoustonR <@t> chi.osu.edu Fri Jan 26 12:39:20 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Fri Jan 26 12:39:49 2007 Subject: [Histonet] histology vacancy Columbus Children's Hospital Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77CEE8@chi2k3ms01.columbuschildrens.net> Columbus Children's Hospital, recently named in the top 10 pediatric hospitals in the nation, has a vacancy in our Anatomic Pathology department for a registered or registry eligible HT. Experience in IHC and/or EM an advantage. Please visit our web-site, http://www.childlab.com/, for more information on the lab and http://www.columbuschildrens.com/ to apply: Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Charles.Embrey <@t> carle.com Fri Jan 26 12:47:30 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Jan 26 12:47:39 2007 Subject: [Histonet] Hope someone out there can let me know what you do. I washaving an issue of numbers coming off the Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4A8@EXCHANGEBE1.carle.com> I don't rinse after acetic acid and have no problem. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate Mendell Sent: Friday, January 26, 2007 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope someone out there can let me know what you do. I washaving an issue of numbers coming off the Hope someone out there can let me know what you do. I was having an issue of numbers coming off the cassettes during processing. After trying different thing for the last two months the PA informed me that Carnoys was being used (to set the ink) and was not always rinsed off. This may also explain the problem I was having with my recycler (formalin). The PA switch to 4% acetic acid, blocks are better (a little) but she said she still isn't rinsing and there is no need to that others out there are not rinsing. What do others do. Kate ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Temple <@t> ssfhs.org Fri Jan 26 08:01:33 2007 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Fri Jan 26 12:57:06 2007 Subject: [Histonet] Grossing Stations Message-ID: When we remodeled our lab, we put in Mopec grossing stations. The company is great to work with. The stations can be built to suit your needs. We have one, I believe it is the 600, that is height adjustable. The other unit is stationary. Ventilation works well on both. Would highly recommend Mopec. Nancy Temple Supervisor Histology/Cytology St. Francis Hospital Indianapolis, In -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Thursday, January 25, 2007 6:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Stations All, We are considering purchasing two large grossing stations with some extra options. Can any of you recommend the grossing stations that you would prefer. We would like the grossing station to last for a reasonable amount of time, be sturdy in construction, offer options that can be added later but also be user friendly with enough shelf space and work area. Most importantly the ventilation must be of high quality. We have several companies that we are looking at but would like to get an idea of what most folks are using and if you are happy with the units or not. If you could also tell us what features you liked. Thank you in advance, Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From Janice.Fightmaster <@t> HCAhealthcare.com Fri Jan 26 14:31:13 2007 From: Janice.Fightmaster <@t> HCAhealthcare.com (Fightmaster Janice) Date: Fri Jan 26 14:30:52 2007 Subject: [Histonet] (no subject) Message-ID: Looking for PCP control slides or blocks. If you can help please contact me. Thanks! Janice Fightmaster Pathology Supervisor Oak Hill Hospital 11375 Cortez Blvd. Brooksville, FL 34613 (352)597-6363 Ext. 3636 From ngianotti <@t> g-com.com Fri Jan 26 16:24:20 2007 From: ngianotti <@t> g-com.com (Nancy Gianotti) Date: Fri Jan 26 16:25:04 2007 Subject: [Histonet] peloris processing Message-ID: <45BA7F94.8060609@g-com.com> Can anyone who has a peloris processor please tell me what your processing times are for many blocks of breast tissue, put in penfix for 2 hours and then put on the processor. Also, what the stirrers are set at medium or high. thanks for your help Nancy Gianotti From Sjohnso616 <@t> aol.com Fri Jan 26 16:29:49 2007 From: Sjohnso616 <@t> aol.com (Sjohnso616@aol.com) Date: Fri Jan 26 16:30:06 2007 Subject: [Histonet] Keratin Artifact Message-ID: Has anyone experienced the following artifact? When performing Keratin IHC stains from previously frozen lymph node tissue in paraffin blocks. I am seeing plasma cells and the occasional histiocyte showing light but positive staining. It is only recently that we have seen this happen. The same artifact is reproducible using Bectin -Dickinson's pre-dilute Cam 5.2 diluted to 1:20 and 1:40 among different lot #'s and unopened antibody vials to eliminate contamination. Instrumentation utilized is the Dako autostainer and Vision BioSystems Bond instrument. The same staining pattern is also seen when stained on Ventana's Nexes instrument using the same Cam 5.2 and again a new unopened antibody. It is also seen when staining on Ventana's Benchmark XT instrument using Ventana's Keratin 5D3 antibody. This seems to happen mostly but not always on tissue previously used for frozen section snap frozen with O.C.T. The frozen tissue is thawed and placed in unbuffered zinc formalin and processed. Comparative studies using NBF netted same results. Keratin stains performed on colon tissue or similar tissue not frozen but processed in the same processor does not show this artifact. Any ideas of what might be the cause would be appreciated. Could this have something to do with radiation procedures of lymphoscintigraphy ? Thanks, James M. Hart Sarasota Pathology Sarasota, Florida From Eric <@t> ategra.com Fri Jan 26 17:37:44 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Jan 26 17:48:04 2007 Subject: [Histonet] Updated list of Histology opportunites near you - Should I call you to discuss? (Update 1/26/07) Message-ID: Hi - Histonetters - I have both permanent and full time (permanent) jobs for HistoTechs throughout the US. Do you know that I can speak to about these HistoTech jobs ? Should I call you to discuss? These permanent and temporary HistoTech jobs are being filled quickly, don't miss out on your dream job- call today! Histology Temporary Assignments (Updated Jan 11 2007) ------------------------------------------------------- 1. California (Palm Springs) - Hot-Hot- Histotech with ISH experience- 13 weeks- Needs to be filled by 1/19/07- Filled 2. Hot ! - Southern Texas, Great Location - Histotech-Must have IHC experience - Call Today- Needs To Be Filled By 1/15/07 (Still looking) 3. Texas (Southwest) - 13 weeks -Histo Tech- Filled Permanent Histology Jobs: (Updated Jan 26 2007) ------------------------------------------------------- New Permanent Jobs Listed below ------------------------------------------------------- 1. Central Ohio- Senior Pathology Assistant - AAPA is Required 2. Central Ohio - A.P Manager - ASCP is required 3. Southern Virginia- Histotech - Bench - Great Location, Great pay- call now! 4.Florida( Tampa Bay area) Histotech- bench- perm 5.Massachusetts (Greater Boston Area) Histotech- Bench - perm 6.Northern Ohio - Histotech - Bench- perm 7. North Carolina (Triangle) - Histotech - Bench - Perm ------------------------------------ Older Jobs I have available ---------------------------------- 01. Central Virginia- Histotech- perm 02. Southern California- Histotech- perm 03. Southern Idaho- Histotech - perm BrandNew- Filled 04. Central Florida - Histotech with some MOHS experience - perm - Hot 05. Southern Alabama - Histotech - basic histology with opportunity to learn new and non-routine Histology -perm- Filled 06. Southeast South Dakota- Histotech - Must have ties to South Dakota- perm- Hot! 07. Southwest Florida- Histotech Supervisor- perm - BrandNew (Need Florida License)-Filled 08. Southwest Texas- Bench Histotech- perm - BrandNew-Filled 09. Hot! Eastern, Massachusetts Seeking Histotechs of all experience levels, Great Pay, Location And Benefits- Call Today - 15% Pay Raise Guaranteed! 10. NEWJOB! Southeast Florida- Histotech - perm - (Need Florida License) 11. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 12. Northern New Jersey - Histotech - perm-Filled 13. Eastern Mass - Histotech - one Senior Histotech, One not so Senior Histotech- Filled 14. Eastern Mass - Histotech - Histotech -perm- Filled 15. Massachusetts (North of Boston) - perm - Bench Histotech- Filled 16. Central Florida -perm- Histotech (Need Florida License) 17. Southeast Florida - Treasure Coast - perm - Histotech (Need Florida License)- Filled 18. Southeast Florida - perm - Histotech (Need Florida License)-Filled 19. Florida, West Coast - both temp & perm openings- Bench Histotech -Very-Hot 20. New York ( Syracuse area) - Bench Histotech- perm 21. New York City (Long Island) - Bench- perm 22. Central Florida - Bench Histotech- perm 23. Las Vegas - Bench Histotech- perm 24. Wisconsin- Histology Supervisor- BrandNew -Hot Hot Hot 25. Central-Illinois-Bench-Dermpath- BrandNew -Hot 26. Northern California- Bench- Routine Histology- BrandNew -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From pruegg <@t> ihctech.net Sat Jan 27 18:44:45 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Jan 27 18:44:55 2007 Subject: [Histonet] pens In-Reply-To: <45B73ABD0200008C00036DF4@GWIA02.HERSHEYMED.NET> Message-ID: <200701280044.l0S0idwd077072@pro12.abac.com> We use pens from StatLabs we like, just have to let the cassettes dry a minute or two before putting them in alcohol waiting for processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 8:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jan 27 19:07:21 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Jan 27 19:07:33 2007 Subject: [Histonet] Re: HER2 and the 48 hour rule In-Reply-To: Message-ID: <200701280107.l0S17F2i084702@pro12.abac.com> I think CAP is addressing the issue of under fixed samples by saying that your methods must be compared or validated against methods using a minimum standard formalin fixation, if you do not fix long enough to protect the sample from alcohol and xylene processing I don't think the samples are going to stand up in this comparison with adequately formalin fixed samples then paraffin processed which you have to demonstrate. Alcohol and glycol fixatives are a problem for her2 as it really loves alcohol and you may get more positives when compared with adequate formalin fixation which should be used as the standard. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Tuesday, January 23, 2007 5:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: HER2 and the 48 hour rule Here's a truly radical solution: if the pathologist on call comes in on Saturday (or Sunday) morning, let the pathologist embed the breast biopsies! It's about time pathologists learned to embed. Short of that revolutionary approach, I'd want the tissue to fix overnight, then be passed to 70% alcohol, then processed Sunday night. - But it isn't known that this approach works, and it doesn't comply with the rules (which aren't new, actually). But it's got to be preferable to leaving the specimen in xylene (or substitute) or in molten wax. Don't forget the issue of non-formalin fixatives, including glyoxal - they haven't been tried. I'm more concerned about tissue that isn't left in formalin for long enough. If it's not, it fixes in the processor in alcohol. I'm even more concerned about delay in fixation. In many radiology services, stereotactic and wire-localization specimens aren't placed in formalin until after specimen radiography is completed and the specimen is leisurely taken to the pathology lab. I was appalled, but not surprised, by the CAP's failure to address any of these issues. Let's remind ourselves what's at stake here. HER2 status determines whether a patient is treated with trastuzumab (Herceptin), a $50,000 treatment with a potential for damaging the heart. Estrogen receptor determination is also affected by fixation, and expensive and hazardous treatment depends on that also. The lack of concern about this issue by primary care physicians, surgeons, and oncologists once again underlines the "redhaired stepchild" status of anatomic pathology in the laboratory and in the health care system. Remember that when you read the newspapers and magazines and read that faceless "technicians" are responsible for all of these procedures. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jan 27 19:11:12 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Jan 27 19:11:21 2007 Subject: [Histonet] Mouse Lens cryosectioning In-Reply-To: <000001c73f44$90a5cee0$0d00a8c0@domain.Premier> Message-ID: <200701280111.l0S1B6Es085930@pro12.abac.com> I do a lot of frozen sections on rabbit corneas and the best method I have found includes overnight fixation in 10% NBF followed by rinses in pbs and then infiltration overnight again in 30% sucrose, I then snap freeze the samples and cut at about -18 as Liz suggested. I do not get any cracks, not done this with murine though. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, January 23, 2007 4:17 PM To: 'David Park'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mouse Lens cryosectioning David How cold is your cryostat? I would try to section in the warmest cryostat as possible, possibly around -18 or so. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Park Sent: Tuesday, January 23, 2007 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse Lens cryosectioning Dear Histonetters, We have been doing some cryosectioning of murine lenses for immunohistochemistry, and have been having much difficulty in getting good looking sections. Of particular problem is the shattering of the lens, especially towards the center. We have attempted a variety of different methods, including frozen sections that are post-fixed with methanol and acetone and previously fixing the lens in 4% paraformaldehyde for an hour. Are there any other kinds of fixative that you may recommend? I have heard of Davidson's fixative and was wondering whether this would disrupt the actual IHC stainings. We have also tried 4% fixation, and while the lens itself looks good when initially cut, giant cracks begin to form over a short period of time while the slides have been in the cryostat. I have also tried leaving the slides out at room temperature after doing sections to see if the shattering is reduced, but the staining was completely gone, I think possibly because the cytoplasm may have leaked out as a result. Any kind of recommendation, techniques, and so forth are definitely welcome. Thanks. David Park szero1009@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jan 27 19:14:32 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Jan 27 19:14:40 2007 Subject: [Histonet] HER2 and the 48 hour rule In-Reply-To: <637089.4652.qm@web61214.mail.yahoo.com> Message-ID: <200701280114.l0S1EQcx086992@pro12.abac.com> If you truly fixed in formalin for 48hrs. wouldn't it be SAFE to leave the sample in 70% alcohol until processing, adequate formalin fixation will protect the sample from alcohol damage to the her2 protein. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 23, 2007 1:43 PM To: Douglas D Deltour; 'sheila adey'; ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: RE: [Histonet] HER2 and the 48 hour rule When you start commercial production, please send me the price per gallon! Ren? J. Douglas D Deltour wrote: I have just patented (in my mind) the idea of "HER2-Safe". It is a holding liquid that you can load in your processor. It will be pumped into your retort after the 48 hours of formalin fixation. It will sit in this safe liquid until you are ready to begin normal tissue processing. This liquid will not harm your tissue and it is within CAP guidelines for HER2 testing. No need for your techs to come in over the weekend! Can I get a witness! I am opening up bidding for rights to this idea. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, January 23, 2007 2:36 PM To: ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] HER2 and the 48 hour rule This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7 207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Sun Jan 28 12:44:20 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sun Jan 28 12:44:30 2007 Subject: [Histonet] HER2 and the 48 hour rule References: <200701280114.l0S1EQcx086992@pro12.abac.com> Message-ID: <000b01c7430c$52f847a0$6500a8c0@mainbox> Yes, it would!! Bryan ----- Original Message ----- From: "patsy ruegg" To: "'Rene J Buesa'" ; "'Douglas D Deltour'" ; "'sheila adey'" ; ; <1dpeterson@meriter.com>; Sent: Saturday, January 27, 2007 8:14 PM Subject: RE: [Histonet] HER2 and the 48 hour rule If you truly fixed in formalin for 48hrs. wouldn't it be SAFE to leave the sample in 70% alcohol until processing, adequate formalin fixation will protect the sample from alcohol damage to the her2 protein. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 23, 2007 1:43 PM To: Douglas D Deltour; 'sheila adey'; ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: RE: [Histonet] HER2 and the 48 hour rule When you start commercial production, please send me the price per gallon! Ren? J. Douglas D Deltour wrote: I have just patented (in my mind) the idea of "HER2-Safe". It is a holding liquid that you can load in your processor. It will be pumped into your retort after the 48 hours of formalin fixation. It will sit in this safe liquid until you are ready to begin normal tissue processing. This liquid will not harm your tissue and it is within CAP guidelines for HER2 testing. No need for your techs to come in over the weekend! Can I get a witness! I am opening up bidding for rights to this idea. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, January 23, 2007 2:36 PM To: ploykasek@phenopath.com; 1dpeterson@meriter.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] HER2 and the 48 hour rule This prompts a new question. I'm curious as to whether other labs that don't work weekends leave their tissues in formalin with a delayed start or do you start the processor right away and leave the tissues in Xylene as stated below? Sheila Adey HT MLT Port Huron Hospital Michigan >From: Patti Loykasek >To: "Peterson, Dan" <1dpeterson@meriter.com>,histonet > >Subject: Re: [Histonet] HER2 and the 48 hour rule >Date: Tue, 23 Jan 2007 10:06:08 -0800 > >Dear Dan, >Welcome to the new world of Her2 testing! There will definitely be changes >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all >techs to get a copy of the guidelines and read them. >That being said, the time in fixation is important for Her2 testing. With >heat retrieval I think that too little fixation is worse than >48 hours, >but >for now we must comply with 6-48 hours. I was taught (a long time ago) that >it is best practice for the weekend schedule fixation to reflect the rest >of >the week, and that it is preferable to leave the tissue longer in xylene. >That xylene would have less bad effects than longer time in formalin. A >longer time in alcohol would be deleterious to many IHC stains, and not >recommended for Her2. >Whatever schedule changes you decide to implement, hopefully you can test >your new schedule on some tissue before full implementation of a new >processing schedule. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > > > Hello Histonetters! > > Maybe this has been discussed before, and if it has, I apologize in > > advance, but I need a little input. Now that the CAP has mandated a 6 > > -48 hour window of fixation time for specimens that may have Her-2 neu > > performed, what are you doing about weekend specimens? We JUST (after > > 25+ years) got rid of the need for techs to come in on Saturdays, and > > would like to be able to continue this trend. However if a breast bx is > > done at an outside account on a Thursday afternoon, and does not get > > grossed by our staff until Friday, right now a our processors are set to > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So > > here are my questions: > > Do you set up the processor to sit in 70% OH after the formalin fix? Do > > you let it sit in the paraffin from Saturday until Monday? (Is heat too > > prolonged?) > > Or do I have to break the news to staff that if their name is up, and a > > breast bx comes in, they're coming in Saturday am? > > We do not have a microwave processor (yet), but soon. > > Any and all responses will be greatly appreciated! > > > > Daniel R Peterson HT(ASCP) > > Histopathology Section Head > > Meriter Laboratories > > (608)-267-6557 > > 1dpeterson@meriter.com > > > > CONFIDENTIALITY NOTICE: This message (including any attachments) is > > intended for the sole use of the individual and entity to whom it is > > addressed. This message may contain information that is confidential and > > is protected by law. If you are not the intended recipient, you are > > hereby notified that any disclosure, copying or distribution of this > > message is strictly prohibited. If you received this message in error, > > please immediately notify the sender by reply email and then delete the > > message. Thank you. > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7 207d94d45&mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmikita <@t> wmcnet.org Mon Jan 29 06:33:11 2007 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Mon Jan 29 06:33:49 2007 Subject: [Histonet] Hope someone out there can let me know what you do. I was Message-ID: Hello, Our PA uses Acetone to fix the ink on the tissue, we just submerge the inked tissue in it for a couple of second. When he cuts the tissue the acetone evaporates before he gets it into the blocks. We are using the Surgipath cassette markers, and have found that if the blocks don't get any formalin (or very little) on the processor, the markers do come off. But have not had any problems with them coming off if the get atleast a half hour on the processor. I have no idea why they come off if not, because they set in formalin, for hours prior to going on the processor. TTYL, Daryl Mikita, HT(ASCP)cm From pkarlisch <@t> psu.edu Mon Jan 29 08:18:07 2007 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Mon Jan 29 08:18:40 2007 Subject: [Histonet] pens References: <45B73ABD0200008C00036DF4@GWIA02.HERSHEYMED.NET> <200701280044.l0S0idwd077072@pro12.abac.com> Message-ID: <20070129T091807Z_D9B900000000@psu.edu> All, I have received good recommendations for cassette pens and it looks like there are many more pens out there then I knew about. We will try each one and see if this alleviates the smudging. Thank you all so much. Pat Karlisch. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "patsy ruegg" 1/27/2007 7:44 PM >>> We use pens from StatLabs we like, just have to let the cassettes dry a minute or two before putting them in alcohol waiting for processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 8:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge <@t> aol.com Mon Jan 29 10:18:46 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Mon Jan 29 10:18:58 2007 Subject: [Histonet] Mouse Testes Fixation and Processing Message-ID: <8C911D8497047E2-1C5C-2A4@FWM-M33.sysops.aol.com> Hello All. I am having an ongoing problem with formalin fixed, paraffin embedded whole mouse testes (Adult mouse and Pups). I need to get this issue resolved and have consistent results for my investigators. They are using these testis for xgal, IF, IHC and In Situ. All my Bouin's fixed testes looks beautiful but the formalin fixed testes are horrible. Bouins is not suitable for much of what they are doing. I have tried several processing schedules and nothing seems to work. Sometimes the FFPE's seem very dry and shrunk, sometimes the cells look like they have big halos around them and you can't distinguish them enough for staging. This is frustrating! All other tissue seems to be fine except these and there is a big variability in how these testes turn out. They are NBF or cold 4%PFA PBS fixed for 24 hours. Typical processing schedule is either 30 minutes each station or 1 hour each station (formalin, 2x70%, 80%, 2x95%, 2x100% reagent alcohol, 2x Xylene, 3x Ameraffin Paraffin (Cardinal cat# M7346-1A). Donna J. Emge, HT(ASCP) Northwestern University Lurie 7-220 303 E. Superior Avenue Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From GMartin <@t> marshallhospital.org Mon Jan 29 11:15:00 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Mon Jan 29 11:13:02 2007 Subject: [Histonet] Xylene substitute Message-ID: We presently use Xylene in our processor. We will be moving to a Xylene substitute. We are a small lab and still hand cover all of our slide. In the cover slip line we do use Sugipath's Sub-x Xylene substitute and are planning on using Sub-x. Can anyone in the group fill me in on their experience with a change over to a Xylene substitute. Thank you Gary Martin El Dorado pathology California. From dcrippen <@t> buckinstitute.org Mon Jan 29 11:27:46 2007 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Mon Jan 29 11:27:56 2007 Subject: [Histonet] Immunogold question Message-ID: Dear EM experts, I've been using the following protocol for immunogold labeling for the past couple years with good success. This morning one of our users has inquired as to why the secondary is incubated at a higher pH than the primary. I do not know the answer...do any of you??? IMMUNOGOLD STAINING TECHNIQUE Cut sections (preferably processed into acrylic resin) and mount on inert grids such as nickel or gold. Rinse grids in distilled water for 10 minutes. Incubate in pH 7.4 T.B.S. ( tris buffered saline) containing 5% normal serum for 30 minutes. (Serum from same animal as secondary antibody). The concentration of the normal serum may have to be increased to up to 50% to prevent background signal. Incubate in specific primary antibody diluted 1 in 5 with pH 7.4 T.B.S., including 0.1% bovine serum albumin, for 30 minutes. (Check pH after preparation). Wash grids in two changes of pH 7.4 T.B.S. for 5 minutes each, then two changes of pH 8.2 T.B.S. for 5 minutes each. Incubate with immunogold conjugated secondary antibody diluted 1 in 50 with pH 8.2 T.B.S., including 0.8% bovine serum albumin, for 1.5 hours. Wash grids in pH 8.2 T.B.S. for 5 minutes x 2. Post fix grids in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer for 15 minutes. Wash grids in two changes of distilled water for 5 minutes each. Stain grids with uranyl acetate and lead citrate. (If using LR White? resin stain in aqueous uranyl acetate.) Many thanks in advance!! Danielle Crippen Morphology and Imaging Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org From djemge <@t> aol.com Mon Jan 29 11:52:11 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Mon Jan 29 11:52:33 2007 Subject: [Histonet] Re: Whole Mouse Testes Message-ID: <8C911E556133E16-ABC-1A86@WEBMAIL-RA18.sysops.aol.com> Thank you everyone for such great help. Hiro if I drive over to U of I at Chicago who would I get in contact with? Everyone so far seems to unanimously think I should focus on the fixation. I am open to follow anyones successful technique since I want to get consistent results. ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From sheila_adey <@t> hotmail.com Mon Jan 29 12:38:44 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Jan 29 12:39:02 2007 Subject: [Histonet] Xylene substitute In-Reply-To: Message-ID: We tried using formula 88 and found it to be also irritating, especially after a spill. It was a problem when we tried to coverslip because our sugipath mounting media is Xylene based but since you have already covered that issue, it would be nice to hear what you think of the quality of the product. Please share after you've been using it. Sheila Adey HT MLT Port Huron Hospital Michigan >From: GMartin@marshallhospital.org >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Xylene substitute >Date: Mon, 29 Jan 2007 09:15:00 -0800 > >We presently use Xylene in our processor. We will be moving to a Xylene >substitute. We are a small lab and still hand cover all of our slide. In >the cover slip line we do use Sugipath's Sub-x Xylene substitute and are >planning on using Sub-x. >Can anyone in the group fill me in on their experience with a change over >to a Xylene substitute. >Thank you >Gary Martin >El Dorado pathology >California. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA From lpaveli1 <@t> hurleymc.com Mon Jan 29 12:41:25 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Jan 29 12:42:18 2007 Subject: [Histonet] Xylene substitute Message-ID: <45BDF985020000EE00010CF6@smtp-gw.hurleymc.com> Hi Gary, We recenty had a change over to a substitute and went with the aliphatic Propar by Anatech. Easy transition, very low oder, docs didn't notice (big plus). We did add an extra container on the stainer when depariffinizing, and on the processor, we have 3 containers of the substitute. We are also able to recycle it. Works well with hand coverslipping media also. You'll be happy with the change. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> 01/29/07 12:15 PM >>> We presently use Xylene in our processor. We will be moving to a Xylene substitute. We are a small lab and still hand cover all of our slide. In the cover slip line we do use Sugipath's Sub-x Xylene substitute and are planning on using Sub-x. Can anyone in the group fill me in on their experience with a change over to a Xylene substitute. Thank you Gary Martin El Dorado pathology California. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Jan 29 13:51:14 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Jan 29 13:54:29 2007 Subject: [Histonet] pens References: <45B73ABD0200008C00036DF4@GWIA02.HERSHEYMED.NET><200701280044.l0S0idwd077072@pro12.abac.com> <20070129T091807Z_D9B900000000@psu.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A73C@fhosxchmb006.ADVENTISTCORP.NET> We have been looking for a similar pen that will stay on the cassettes AND the slides. Right now we use two different pens. Smudging has been a problem, but the Mercedes pens seem like a winner! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patricia Karlisch Sent: Mon 1/29/2007 9:18 AM To: Histonet@lists.utsouthwestern.edu; ruegg, patsy; Webb', 'Dorothy L Subject: RE: [Histonet] pens All, I have received good recommendations for cassette pens and it looks like there are many more pens out there then I knew about. We will try each one and see if this alleviates the smudging. Thank you all so much. Pat Karlisch. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "patsy ruegg" 1/27/2007 7:44 PM >>> We use pens from StatLabs we like, just have to let the cassettes dry a minute or two before putting them in alcohol waiting for processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 8:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From algranth <@t> u.arizona.edu Mon Jan 29 14:33:12 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Jan 29 14:37:10 2007 Subject: [Histonet] Miller's Elastic Stain Message-ID: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Happy Monday! I have had a request to do a Miller's Elastic Stain. I have almost all of the stains and I do have all of the chemicals to do the stain but I have a question. One of the ingredients in the elastic stain is resorcin. I'm not a chemist so I have to ask this question, I have resorcinol in my chemical cabinet - is this the same thing as resorcin? I can find resorcinol in the Sigma cat. but not resorcin. Thanks! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From gamal.akabani <@t> gmail.com Mon Jan 29 14:40:39 2007 From: gamal.akabani <@t> gmail.com (Gamal Akabani) Date: Mon Jan 29 14:40:55 2007 Subject: [Histonet] Hypoxia Message-ID: <4885123C-E70A-41BC-AAE0-1F483B088D7A@gmail.com> Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal From PMonfils <@t> Lifespan.org Mon Jan 29 14:50:40 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jan 29 14:50:50 2007 Subject: [Histonet] Miller's Elastic Stain In-Reply-To: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C0E@LSRIEXCH1.lsmaster.lifespan.org> Yes, resorcinol and resorcin are the same thing, C6H6O2 From tkngflght <@t> yahoo.com Mon Jan 29 14:53:02 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Mon Jan 29 14:53:11 2007 Subject: [Histonet] If you are even remotely curious, please call! Even more temp openings-- In-Reply-To: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> References: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Message-ID: <00b501c743e7$77b140c0$6401a8c0@FSDESKTOP> Hi All! We're swamped and need more great techs!! If you have ever been ever remotely curious about travel temps, I would love to talk with you. Even if you won't be ready for a year or more--let's start the conversation so when you are ready to go, you're comfortable and happy in your decision. I keep in touch. I don't pressure you (it is YOUR life, right?) Someone said I don't come across as a used car salesman.I'm a histotech!! If something doesn't fit--you tell me 'no' and we move on to the next opening. Easy as that. I have EIGHT open positions and need to fill them within the next two weeks. All shifts, all skills. With the changes in the registry and state licensing, we're going to continue to need great techs who want to try new things. Give me a call--it's an investment of about 15 minutes for the basics. I help new travelers get started and we've got a great team. It all starts with a question- "So, tell me about temping?" I'll give you the details of the jobs that fit when we get a chance to chat!! Cheryl :-) Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 800.756.3309 From bhewlett <@t> cogeco.ca Mon Jan 29 14:54:58 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Jan 29 14:55:13 2007 Subject: [Histonet] Miller's Elastic Stain References: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Message-ID: <000d01c743e7$bd2f0470$6500a8c0@mainbox> Andi, Resorcin is the same thing as resorcinol! Bryan ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 3:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew <@t> shaw.ca Mon Jan 29 14:43:26 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Jan 29 15:02:57 2007 Subject: [Histonet] Miller's Elastic Stain References: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Message-ID: <001201c743e6$209f0520$6400a8c0@yourlk4rlmsu> Same thing. Bryan Llewellyn ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 12:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> u.arizona.edu Mon Jan 29 15:19:22 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Jan 29 15:19:40 2007 Subject: [Histonet] Miller's Elastic Stain In-Reply-To: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.ari zona.edu> Message-ID: <4.3.2.7.2.20070129141822.00e1a5e0@algranth.inbox.email.arizona.edu> Thanks! Glad to hear that it is the same thing. Looks like I'm good to go. Andi At 01:33 PM 1/29/2007 -0700, Andrea Grantham wrote: >Happy Monday! >I have had a request to do a Miller's Elastic Stain. I have almost all of >the stains and I do have all of the chemicals to do the stain but I have a >question. >One of the ingredients in the elastic stain is resorcin. I'm not a chemist >so I have to ask this question, I have resorcinol in my chemical cabinet - >is this the same thing as resorcin? I can find resorcinol in the Sigma >cat. but not resorcin. > >Thanks! >Andi Grantham >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From christina.thurby <@t> bms.com Mon Jan 29 15:20:56 2007 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Mon Jan 29 15:21:09 2007 Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? In-Reply-To: <0JCN00H0E632BS@chimera.bms.com> References: <0JCN00H0E632BS@chimera.bms.com> Message-ID: <45BE6538.4090202@bms.com> Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina From babistace52 <@t> yahoo.com Mon Jan 29 15:30:17 2007 From: babistace52 <@t> yahoo.com (Stasha McCollum) Date: Mon Jan 29 15:30:25 2007 Subject: [Histonet] ASCP Exam Message-ID: <20070129213017.85649.qmail@web35009.mail.mud.yahoo.com> Can anyone tell me if the practical portion of the certification exam has been remove? Ther were rumors floating about that change last year at this time. Also if anyone has taken it recently what is the percentage of IHC on the exam Thanks --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From GMartin <@t> marshallhospital.org Mon Jan 29 15:44:41 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Mon Jan 29 15:42:40 2007 Subject: [Histonet] Processing debate Message-ID: We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California From nizarlim <@t> gmail.com Mon Jan 29 15:56:26 2007 From: nizarlim <@t> gmail.com (nizar limaiem) Date: Mon Jan 29 15:56:35 2007 Subject: [Histonet] a question Message-ID: <92805bf10701291356j3e107923md8a75f1a0929f8a7@mail.gmail.com> Dear Doctor I am student in biotechnology (Tunisia), I work on the developpement of the Embryo and Early Larva of a fish species dicentrarchus labrax L (it's egg is about 1mm in diameter). I want to use methods of histology on fertilized eggs. Could some one offer some suggestions on the experimental protocol (fixation/processing/sectioning) Thank you for your reply limaiem nizar. Student in marin biotechnology University of biotechnology, Monastir, Tunisise From ryakay <@t> shands.ufl.edu Mon Jan 29 16:08:03 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Mon Jan 29 16:08:37 2007 Subject: [Histonet] ASCP Exam Message-ID: Hi, Yes, the practical portion of the exam has been done away with. I had a student that just took the exam. There are many more troubleshooting questions now on all aspects of histology. My recommendations based on what the student told me is that you need to really focus on troubleshooting of special stains as well as fixation and cutting. As far as I can tell, the study guide has not been updated and I don't know if it will be. Good luck, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> Stasha McCollum 01/29/07 4:30 PM >>> Can anyone tell me if the practical portion of the certification exam has been remove? Ther were rumors floating about that change last year at this time. Also if anyone has taken it recently what is the percentage of IHC on the exam Thanks --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Jan 29 16:36:54 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Jan 29 16:28:15 2007 Subject: [Histonet] human total IgG Message-ID: <000001c743f5$f9f72280$0d00a8c0@domain.Premier> Hello everyone Does anyone out there know of an antibody that will detect total IgG for human tissue in paraffin sections. I have searched the web and have been unable to come up with one that will detect total IgG so any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From PMonfils <@t> Lifespan.org Mon Jan 29 16:29:30 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jan 29 16:29:40 2007 Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? In-Reply-To: <45BE6538.4090202@bms.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C11@LSRIEXCH1.lsmaster.lifespan.org> Yep, it means the same thing for antibodies that it means for scotch - undiluted - straight from the bottle. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Christina Thurby > Sent: Monday, January 29, 2007 1:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? > > <> > > Hello Histonet Folks!, > Is anyone out there performing flow cytometry that can tell me what a > suggested working dilution "Neat" means. I think it is the use of an > 'undiluted' primary antibody but if someone familiar with this term can > please confirm that I'd be very appreciative. > Thanks, > Christina > > > From graham.brown <@t> ttuhsc.edu Mon Jan 29 16:37:31 2007 From: graham.brown <@t> ttuhsc.edu (Brown, Graham W) Date: Mon Jan 29 16:37:43 2007 Subject: [Histonet] MCP1 positive control Message-ID: <7F7DE44DEE269C4CB34BB8EADE0525EC61E472@BOWIE.ttuhsc.edu> Does anyone know of a positive control tissue for MCP1?? thanks Graham Brown Texas Tech University HSC Garrison Institute on Aging Dept. Neurology Lubbock, TX From laurie.reilly <@t> jcu.edu.au Mon Jan 29 17:19:15 2007 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Mon Jan 29 17:19:28 2007 Subject: [Histonet] Mouse Testes Fixation and Processing In-Reply-To: <8C911D8497047E2-1C5C-2A4@FWM-M33.sysops.aol.com> Message-ID: <5.2.0.9.0.20070130090758.00bea1f0@mail.jcu.edu.au> Donna and All, Bouin's is the fixative of choice for testis, and my experience with formalin fixation of testis tells me that your observations are correct. A formalin-acetic acid-ethanol mix may be better. Maybe Davidson's fixative? Another "trick of the trade" is to start the processing of testis (and other very delicate tissues) in 30% Ethanol then 50%, 70%, 80%, 90%, 95%, 100%, 100% then 50:50 EtOH:Xylene, 2x Xylene 3x Paraffin. For mouse tissues 15 minutes in each of these reagents would be enough. Regards, Laurie. At 11:18 AM 29/01/2007 -0500, djemge@aol.com wrote: >Hello All. I am having an ongoing problem with formalin fixed, paraffin >embedded whole mouse testes (Adult mouse and Pups). I need to get this >issue resolved and have consistent results for my investigators. They are >using these testis for xgal, IF, IHC and In Situ. All my Bouin's fixed >testes looks beautiful but the formalin fixed testes are horrible. Bouins >is not suitable for much of what they are doing. I have tried several >processing schedules and nothing seems to work. Sometimes the FFPE's seem >very dry and shrunk, sometimes the cells look like they have big halos >around them and you can't distinguish them enough for staging. This is >frustrating! All other tissue seems to be fine except these and there is a >big variability in how these testes turn out. They are NBF or cold 4%PFA >PBS fixed for 24 hours. Typical processing schedule is either 30 minutes >each station or 1 hour each station (formalin, 2x70%, 80%, 2x95%, 2x100% >reagent alcohol, 2x Xylene, 3x Ameraffin Paraffin! > (Cardinal cat# M7346-1A). > >Donna J. Emge, HT(ASCP) >Northwestern University >Lurie 7-220 >303 E. Superior Avenue >Chicago, IL 60611 >312-503-2036 >d-emge@northwestern.edu >________________________________________________________________________ >Check out the new AOL. Most comprehensive set of free safety and security >tools, free access to millions of high-quality videos from across the web, >free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 From rchiovetti <@t> yahoo.com Mon Jan 29 17:34:38 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Mon Jan 29 17:34:44 2007 Subject: [Histonet] Immunogold question Message-ID: <825913.37628.qm@web58903.mail.re1.yahoo.com> Hi Danielle, Just a guess here, but the higher pH may help reduce nonspecific background staining. I worked in an EM lab that occasionally played with pH and salt concentrations for immunogold labeling when it was necessary. I know for sure that high salt washes after the primary and secondary Ab's, followed by a return to normal TBS, does a super job of getting rid of background. I'm not so certain about the pH. I only used it a couple of times and I really couldn't tell much difference... Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Danielle Crippen To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 10:27:46 AM Subject: [Histonet] Immunogold question Dear EM experts, I've been using the following protocol for immunogold labeling for the past couple years with good success. This morning one of our users has inquired as to why the secondary is incubated at a higher pH than the primary. I do not know the answer...do any of you??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Want to start your own business? Learn how on Yahoo! Small Business. http://smallbusiness.yahoo.com/r-index From GMartin <@t> marshallhospital.org Mon Jan 29 18:06:53 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Mon Jan 29 18:04:51 2007 Subject: [Histonet] Follow up Message-ID: I'm new to the list and in an effort to keep the list informed I want to thank you for the response to my two questions. 1) concerning Xylene substitutes ... I received a good suggestion on a product FORMULA 83. And I will as suggested ... report back on my success or failures of my transition from Xylene to a substitute. 2) Processing cassettes ... the debate was weather to put them directly into the processing basket or let them float randomly in formalin then put them in the basket. The standard seems to be to load them directly from the grossing table into the processing basket. Thank you all very much Gary Martin El Dorado Pathology From mtarango <@t> nvcancer.org Mon Jan 29 18:31:31 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Jan 29 18:32:03 2007 Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F9951@NVCIEXCH02.NVCI.org> You're right. It means using it straight. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christina Thurby Sent: Monday, January 29, 2007 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From mrsseagle <@t> yahoo.com Mon Jan 29 20:45:11 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Mon Jan 29 20:45:19 2007 Subject: [Histonet] ASCP EXAM Message-ID: <831462.68750.qm@web51812.mail.yahoo.com> I just took HT exam this past december and I dont remember having any IHC related questions on the exam. You just need to know the freida carson book backwards and forwards because they pick the starngest things out of that book that you would not know unless you have just practically memorized the book!! Don't wast your time or money on the ASCP practical exam questions, what a waste. As far as the practical part I am not sure but I believe It has been Removed from the requirements since everthing is so automated now. Good Luck on your Exam Michelle Seagle HT (ASCP) Rutherford Hospital --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From jotahal <@t> epilepsy.biomed.cas.cz Mon Jan 29 23:53:19 2007 From: jotahal <@t> epilepsy.biomed.cas.cz (Jakub Otahal) Date: Tue Jan 30 00:10:53 2007 Subject: [Histonet] Hypoxia Message-ID: <20070130055319.9dbd9a05@epilepsy.biomed.cas.cz> Dear Gamal, have a look at chemicon site (www.chemicon.com) and search for hypoxyprobe. You will have staining kit as well good productsheet describing principles of this method and staining procedure. there is good reference list also. It is good to start with...... Jakub ***************************************** Jakub Otahal MD,PhD Department of Developmental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 14220 Prague 4 jotahal@epilepsy.biomed.cas.cz http://epilepsy.biomed.cas.cz tel: +420 241062495 ***************************************** _____ From: Gamal Akabani [mailto:gamal.akabani@gmail.com] To: Histonet ((E-mail)) [mailto:histonet@lists.utsouthwestern.edu] Sent: Mon, 29 Jan 2007 21:40:39 +0100 Subject: [Histonet] Hypoxia Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HoustonR <@t> chi.osu.edu Tue Jan 30 07:37:27 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Jan 30 07:37:54 2007 Subject: [Histonet] RUO's and diagnostic IHC Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77CEFF@chi2k3ms01.columbuschildrens.net> Here is a reply I received yesterday from Dr Steve Gutman, Director, In Vitro Diagnostics, FDA, regarding the current status of using RUOs in the clinical lab. He did add the disclaimer that these were his views and did not represent official FDA policy; and you thought it was confusing before!!!!! Don't shoot the messenger......... "-----Original Message----- From: Gutman, Steve [mailto:steve.gutman@fda.hhs.gov] Sent: Monday, January 29, 2007 2:52 PM To: Houston, Ronald Subject: RE: RUO's and ASR reagents in diagnostics - FDA disclaimer I have had informal discussions with CAP but FDA has not taken a formal position on use of RUOs to support home brews. In general, we think this is a dreadful choice and clearly a tactic of last resort. There are no regulatory requirements for RUO devices. So the laboratory takes full responsibility for ensuring quality of production of the RUO material being made over time. This seems to me to put laboratories at considerable liability risk since the general controls applied to ASRs don't work here. As we try to review our regulatory policy in this area, it is certainly one we should and will try to clarify. Thanks. Steve ________________________________ From: Houston, Ronald [mailto:HoustonR@chi.osu.edu] Sent: Monday, January 29, 2007 10:23 AM To: Gutman, Steve Subject: RUO's and ASR reagents in diagnostics - FDA disclaimer Importance: High Dr Gutman, There is tremendous confusion in laboratory circles regarding the FDA disclaimer for ASR antibodies and whether or not this same disclaimer can be used for RUO antibodies, particularly in light of CAP's change in interpretation of the guidelines for IHC testing: "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew test only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director. The laboratory must establish or verify the performance characteristics of tests using Class I ASR's and RUO's in accordance with the Method Performance Specifications section of the Laboratory General checklist." Exactly what is the current stance of the FDA, and have any discussions taken place between the FDA and CAP regarding their interpretation? Can results of RUO testing be dictated into a diagnostic report and can the test be billed? Obviously, antibody manufacturers mandate that their RUO antibodies cannot be used for diagnostic purposes, but they are as confused as the laboratorians. Thank you for taking the time to clarify this very confusing situation. Sincerely Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu " Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Tue Jan 30 09:02:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 30 09:03:02 2007 Subject: [Histonet] Processing debate In-Reply-To: Message-ID: <189401.17374.qm@web61213.mail.yahoo.com> Fear the word "random", in histology is almost synonym with "caos".There is absolutely no logic or benefit from placing the cassettes at random. If you use a cassetting log the sequencial and orderly manner of placing the cassettes following the log order is the way of doing things. You can always know where your cassettes are and at the embedding step you can follow the same sequency and easily determine if all the cassettes were processed and embeded. It is absolutely illogical to place anything at random (unless you are selecting something for an experimental design that requires that type of selection!). The only "logical" explanation to your initial question is that it stems from the pathologist's lazyness that prefers to throw the cassettes in the container and letting the histotech to try to organize what should not have been disorganized in the first place. Ren? J. GMartin@marshallhospital.org wrote: We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From kmerriam2003 <@t> yahoo.com Tue Jan 30 09:35:05 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Jan 30 09:35:13 2007 Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Message-ID: <995782.58481.qm@web50313.mail.yahoo.com> Hi Christina, Yes - "neat" means undiluted. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Christina Thurby To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 4:20:56 PM Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091 From Tiffany.Price <@t> thomaswv.org Tue Jan 30 09:53:01 2007 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Tue Jan 30 09:53:08 2007 Subject: [Histonet] RE:xylene substitute and marking pens Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C32139@thm-mail.thomaswv.org> We use the Formula 83 substitute and really like it. It also recycles very well. We use Richard Allen mounting medium to coverslip- it is the most compatible, and recommended by CBG Biotech, the manufacturer of the clearant. We also use SHUR/MARK pens from Triangle Biomedical Sciences- they have a refillable tip that you just replace until the ink runs out of the pen. They come in red or black. I have found that these are the only pens that work with the Formula 83 clearant. Hope this helps! Tiffany -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 30, 2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 38, Issue 46 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Xylene substitute (sheila adey) 2. Re: Xylene substitute (Lynette Pavelich) 3. RE: pens (Bonner, Janet) 4. Miller's Elastic Stain (Andrea Grantham) 5. Hypoxia (Gamal Akabani) 6. RE: Miller's Elastic Stain (Monfils, Paul) 7. If you are even remotely curious, please call! Even more temp openings-- (Cheryl Kerry) 8. Re: Miller's Elastic Stain (Bryan Hewlett) 9. Re: Miller's Elastic Stain (Bryan Llewellyn) 10. Re: Miller's Elastic Stain (Andrea Grantham) 11. Re: Anybody doing flow cytometry? What does Neat mean? (Christina Thurby) 12. ASCP Exam (Stasha McCollum) 13. Processing debate (GMartin@marshallhospital.org) 14. a question (nizar limaiem) 15. Re: ASCP Exam (Kaye Ryan) 16. human total IgG (Liz Chlipala) 17. RE: Re: Anybody doing flow cytometry? What does Neat mean? (Monfils, Paul) 18. MCP1 positive control (Brown, Graham W) 19. Re: Mouse Testes Fixation and Processing (Laurie Reilly) 20. Re: Immunogold question (Robert Chiovetti) 21. Follow up (GMartin@marshallhospital.org) 22. RE: Re: Anybody doing flow cytometry? What does Neat mean? (Tarango, Mark) 23. ASCP EXAM (MICHELLE SEAGLE) 24. Re: Hypoxia (Jakub Otahal) 25. RUO's and diagnostic IHC (Houston, Ronald) 26. Re: Processing debate (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Jan 2007 13:38:44 -0500 From: "sheila adey" Subject: RE: [Histonet] Xylene substitute To: GMartin@marshallhospital.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed We tried using formula 88 and found it to be also irritating, especially after a spill. It was a problem when we tried to coverslip because our sugipath mounting media is Xylene based but since you have already covered that issue, it would be nice to hear what you think of the quality of the product. Please share after you've been using it. Sheila Adey HT MLT Port Huron Hospital Michigan >From: GMartin@marshallhospital.org >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Xylene substitute >Date: Mon, 29 Jan 2007 09:15:00 -0800 > >We presently use Xylene in our processor. We will be moving to a Xylene >substitute. We are a small lab and still hand cover all of our slide. In >the cover slip line we do use Sugipath's Sub-x Xylene substitute and are >planning on using Sub-x. >Can anyone in the group fill me in on their experience with a change over >to a Xylene substitute. >Thank you >Gary Martin >El Dorado pathology >California. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA ------------------------------ Message: 2 Date: Mon, 29 Jan 2007 13:41:25 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Xylene substitute To: , Message-ID: <45BDF985020000EE00010CF6@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Hi Gary, We recenty had a change over to a substitute and went with the aliphatic Propar by Anatech. Easy transition, very low oder, docs didn't notice (big plus). We did add an extra container on the stainer when depariffinizing, and on the processor, we have 3 containers of the substitute. We are also able to recycle it. Works well with hand coverslipping media also. You'll be happy with the change. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> 01/29/07 12:15 PM >>> We presently use Xylene in our processor. We will be moving to a Xylene substitute. We are a small lab and still hand cover all of our slide. In the cover slip line we do use Sugipath's Sub-x Xylene substitute and are planning on using Sub-x. Can anyone in the group fill me in on their experience with a change over to a Xylene substitute. Thank you Gary Martin El Dorado pathology California. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Jan 2007 14:51:14 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] pens To: "Patricia Karlisch" , Histonet@lists.utsouthwestern.edu, "ruegg, patsy" , "Webb', 'Dorothy L" Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A73C@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 We have been looking for a similar pen that will stay on the cassettes AND the slides. Right now we use two different pens. Smudging has been a problem, but the Mercedes pens seem like a winner! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patricia Karlisch Sent: Mon 1/29/2007 9:18 AM To: Histonet@lists.utsouthwestern.edu; ruegg, patsy; Webb', 'Dorothy L Subject: RE: [Histonet] pens All, I have received good recommendations for cassette pens and it looks like there are many more pens out there then I knew about. We will try each one and see if this alleviates the smudging. Thank you all so much. Pat Karlisch. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "patsy ruegg" 1/27/2007 7:44 PM >>> We use pens from StatLabs we like, just have to let the cassettes dry a minute or two before putting them in alcohol waiting for processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 8:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 4 Date: Mon, 29 Jan 2007 13:33:12 -0700 From: Andrea Grantham Subject: [Histonet] Miller's Elastic Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Happy Monday! I have had a request to do a Miller's Elastic Stain. I have almost all of the stains and I do have all of the chemicals to do the stain but I have a question. One of the ingredients in the elastic stain is resorcin. I'm not a chemist so I have to ask this question, I have resorcinol in my chemical cabinet - is this the same thing as resorcin? I can find resorcinol in the Sigma cat. but not resorcin. Thanks! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 5 Date: Mon, 29 Jan 2007 14:40:39 -0600 From: Gamal Akabani Subject: [Histonet] Hypoxia To: "Histonet ((E-mail))" Message-ID: <4885123C-E70A-41BC-AAE0-1F483B088D7A@gmail.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal ------------------------------ Message: 6 Date: Mon, 29 Jan 2007 15:50:40 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Miller's Elastic Stain To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C0E@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Yes, resorcinol and resorcin are the same thing, C6H6O2 ------------------------------ Message: 7 Date: Mon, 29 Jan 2007 14:53:02 -0600 From: "Cheryl Kerry" Subject: [Histonet] If you are even remotely curious, please call! Even more temp openings-- To: Message-ID: <00b501c743e7$77b140c0$6401a8c0@FSDESKTOP> Content-Type: text/plain; charset="US-ASCII" Hi All! We're swamped and need more great techs!! If you have ever been ever remotely curious about travel temps, I would love to talk with you. Even if you won't be ready for a year or more--let's start the conversation so when you are ready to go, you're comfortable and happy in your decision. I keep in touch. I don't pressure you (it is YOUR life, right?) Someone said I don't come across as a used car salesman.I'm a histotech!! If something doesn't fit--you tell me 'no' and we move on to the next opening. Easy as that. I have EIGHT open positions and need to fill them within the next two weeks. All shifts, all skills. With the changes in the registry and state licensing, we're going to continue to need great techs who want to try new things. Give me a call--it's an investment of about 15 minutes for the basics. I help new travelers get started and we've got a great team. It all starts with a question- "So, tell me about temping?" I'll give you the details of the jobs that fit when we get a chance to chat!! Cheryl :-) Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 800.756.3309 ------------------------------ Message: 8 Date: Mon, 29 Jan 2007 15:54:58 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] Miller's Elastic Stain To: , "Andrea Grantham" Message-ID: <000d01c743e7$bd2f0470$6500a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Andi, Resorcin is the same thing as resorcinol! Bryan ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 3:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Mon, 29 Jan 2007 12:43:26 -0800 From: Bryan Llewellyn Subject: Re: [Histonet] Miller's Elastic Stain To: Histonet , Andrea Grantham Message-ID: <001201c743e6$209f0520$6400a8c0@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=response Same thing. Bryan Llewellyn ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 12:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 29 Jan 2007 14:19:22 -0700 From: Andrea Grantham Subject: Re: [Histonet] Miller's Elastic Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20070129141822.00e1a5e0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Thanks! Glad to hear that it is the same thing. Looks like I'm good to go. Andi At 01:33 PM 1/29/2007 -0700, Andrea Grantham wrote: >Happy Monday! >I have had a request to do a Miller's Elastic Stain. I have almost all of >the stains and I do have all of the chemicals to do the stain but I have a >question. >One of the ingredients in the elastic stain is resorcin. I'm not a chemist >so I have to ask this question, I have resorcinol in my chemical cabinet - >is this the same thing as resorcin? I can find resorcinol in the Sigma >cat. but not resorcin. > >Thanks! >Andi Grantham >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 11 Date: Mon, 29 Jan 2007 15:20:56 -0600 From: Christina Thurby Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? To: histonet@lists.utsouthwestern.edu Message-ID: <45BE6538.4090202@bms.com> Content-Type: text/plain; charset="iso-8859-1" Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina ------------------------------ Message: 12 Date: Mon, 29 Jan 2007 13:30:17 -0800 (PST) From: Stasha McCollum Subject: [Histonet] ASCP Exam To: histonet@lists.utsouthwestern.edu Message-ID: <20070129213017.85649.qmail@web35009.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Can anyone tell me if the practical portion of the certification exam has been remove? Ther were rumors floating about that change last year at this time. Also if anyone has taken it recently what is the percentage of IHC on the exam Thanks --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. ------------------------------ Message: 13 Date: Mon, 29 Jan 2007 13:44:41 -0800 From: GMartin@marshallhospital.org Subject: [Histonet] Processing debate To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California ------------------------------ Message: 14 Date: Mon, 29 Jan 2007 22:56:26 +0100 From: "nizar limaiem" Subject: [Histonet] a question To: histonet@lists.utsouthwestern.edu Message-ID: <92805bf10701291356j3e107923md8a75f1a0929f8a7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Doctor I am student in biotechnology (Tunisia), I work on the developpement of the Embryo and Early Larva of a fish species dicentrarchus labrax L (it's egg is about 1mm in diameter). I want to use methods of histology on fertilized eggs. Could some one offer some suggestions on the experimental protocol (fixation/processing/sectioning) Thank you for your reply limaiem nizar. Student in marin biotechnology University of biotechnology, Monastir, Tunisise ------------------------------ Message: 15 Date: Mon, 29 Jan 2007 17:08:03 -0500 From: "Kaye Ryan" Subject: Re: [Histonet] ASCP Exam To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Hi, Yes, the practical portion of the exam has been done away with. I had a student that just took the exam. There are many more troubleshooting questions now on all aspects of histology. My recommendations based on what the student told me is that you need to really focus on troubleshooting of special stains as well as fixation and cutting. As far as I can tell, the study guide has not been updated and I don't know if it will be. Good luck, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> Stasha McCollum 01/29/07 4:30 PM >>> Can anyone tell me if the practical portion of the certification exam has been remove? Ther were rumors floating about that change last year at this time. Also if anyone has taken it recently what is the percentage of IHC on the exam Thanks --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 29 Jan 2007 15:36:54 -0700 From: "Liz Chlipala" Subject: [Histonet] human total IgG To: "'Histonet'" Message-ID: <000001c743f5$f9f72280$0d00a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" Hello everyone Does anyone out there know of an antibody that will detect total IgG for human tissue in paraffin sections. I have searched the web and have been unable to come up with one that will detect total IgG so any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 ------------------------------ Message: 17 Date: Mon, 29 Jan 2007 17:29:30 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C11@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Yep, it means the same thing for antibodies that it means for scotch - undiluted - straight from the bottle. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Christina Thurby > Sent: Monday, January 29, 2007 1:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? > > <> > > Hello Histonet Folks!, > Is anyone out there performing flow cytometry that can tell me what a > suggested working dilution "Neat" means. I think it is the use of an > 'undiluted' primary antibody but if someone familiar with this term can > please confirm that I'd be very appreciative. > Thanks, > Christina > > > ------------------------------ Message: 18 Date: Mon, 29 Jan 2007 16:37:31 -0600 From: "Brown, Graham W" Subject: [Histonet] MCP1 positive control To: Message-ID: <7F7DE44DEE269C4CB34BB8EADE0525EC61E472@BOWIE.ttuhsc.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone know of a positive control tissue for MCP1?? thanks Graham Brown Texas Tech University HSC Garrison Institute on Aging Dept. Neurology Lubbock, TX ------------------------------ Message: 19 Date: Tue, 30 Jan 2007 09:19:15 +1000 From: Laurie Reilly Subject: Re: [Histonet] Mouse Testes Fixation and Processing To: djemge@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <5.2.0.9.0.20070130090758.00bea1f0@mail.jcu.edu.au> Content-Type: text/plain; charset="us-ascii"; format=flowed Donna and All, Bouin's is the fixative of choice for testis, and my experience with formalin fixation of testis tells me that your observations are correct. A formalin-acetic acid-ethanol mix may be better. Maybe Davidson's fixative? Another "trick of the trade" is to start the processing of testis (and other very delicate tissues) in 30% Ethanol then 50%, 70%, 80%, 90%, 95%, 100%, 100% then 50:50 EtOH:Xylene, 2x Xylene 3x Paraffin. For mouse tissues 15 minutes in each of these reagents would be enough. Regards, Laurie. At 11:18 AM 29/01/2007 -0500, djemge@aol.com wrote: >Hello All. I am having an ongoing problem with formalin fixed, paraffin >embedded whole mouse testes (Adult mouse and Pups). I need to get this >issue resolved and have consistent results for my investigators. They are >using these testis for xgal, IF, IHC and In Situ. All my Bouin's fixed >testes looks beautiful but the formalin fixed testes are horrible. Bouins >is not suitable for much of what they are doing. I have tried several >processing schedules and nothing seems to work. Sometimes the FFPE's seem >very dry and shrunk, sometimes the cells look like they have big halos >around them and you can't distinguish them enough for staging. This is >frustrating! All other tissue seems to be fine except these and there is a >big variability in how these testes turn out. They are NBF or cold 4%PFA >PBS fixed for 24 hours. Typical processing schedule is either 30 minutes >each station or 1 hour each station (formalin, 2x70%, 80%, 2x95%, 2x100% >reagent alcohol, 2x Xylene, 3x Ameraffin Paraffin! > (Cardinal cat# M7346-1A). > >Donna J. Emge, HT(ASCP) >Northwestern University >Lurie 7-220 >303 E. Superior Avenue >Chicago, IL 60611 >312-503-2036 >d-emge@northwestern.edu >________________________________________________________________________ >Check out the new AOL. Most comprehensive set of free safety and security >tools, free access to millions of high-quality videos from across the web, >free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 ------------------------------ Message: 20 Date: Mon, 29 Jan 2007 15:34:38 -0800 (PST) From: Robert Chiovetti Subject: Re: [Histonet] Immunogold question To: Danielle Crippen , histonet@lists.utsouthwestern.edu Message-ID: <825913.37628.qm@web58903.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Danielle, Just a guess here, but the higher pH may help reduce nonspecific background staining. I worked in an EM lab that occasionally played with pH and salt concentrations for immunogold labeling when it was necessary. I know for sure that high salt washes after the primary and secondary Ab's, followed by a return to normal TBS, does a super job of getting rid of background. I'm not so certain about the pH. I only used it a couple of times and I really couldn't tell much difference... Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Danielle Crippen To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 10:27:46 AM Subject: [Histonet] Immunogold question Dear EM experts, I've been using the following protocol for immunogold labeling for the past couple years with good success. This morning one of our users has inquired as to why the secondary is incubated at a higher pH than the primary. I do not know the answer...do any of you??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Want to start your own business? Learn how on Yahoo! Small Business. http://smallbusiness.yahoo.com/r-index ------------------------------ Message: 21 Date: Mon, 29 Jan 2007 16:06:53 -0800 From: GMartin@marshallhospital.org Subject: [Histonet] Follow up To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I'm new to the list and in an effort to keep the list informed I want to thank you for the response to my two questions. 1) concerning Xylene substitutes ... I received a good suggestion on a product FORMULA 83. And I will as suggested ... report back on my success or failures of my transition from Xylene to a substitute. 2) Processing cassettes ... the debate was weather to put them directly into the processing basket or let them float randomly in formalin then put them in the basket. The standard seems to be to load them directly from the grossing table into the processing basket. Thank you all very much Gary Martin El Dorado Pathology ------------------------------ Message: 22 Date: Mon, 29 Jan 2007 16:31:31 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? To: "Christina Thurby" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F9951@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii You're right. It means using it straight. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christina Thurby Sent: Monday, January 29, 2007 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 23 Date: Mon, 29 Jan 2007 18:45:11 -0800 (PST) From: MICHELLE SEAGLE Subject: [Histonet] ASCP EXAM To: histonet@lists.utsouthwestern.edu Message-ID: <831462.68750.qm@web51812.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I just took HT exam this past december and I dont remember having any IHC related questions on the exam. You just need to know the freida carson book backwards and forwards because they pick the starngest things out of that book that you would not know unless you have just practically memorized the book!! Don't wast your time or money on the ASCP practical exam questions, what a waste. As far as the practical part I am not sure but I believe It has been Removed from the requirements since everthing is so automated now. Good Luck on your Exam Michelle Seagle HT (ASCP) Rutherford Hospital --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. ------------------------------ Message: 24 Date: Tue, 30 Jan 2007 06:53:19 +0100 From: "Jakub Otahal" Subject: Re: [Histonet] Hypoxia To: histonet@lists.utsouthwestern.edu Message-ID: <20070130055319.9dbd9a05@epilepsy.biomed.cas.cz> Content-Type: text/plain; charset="us-ascii" Dear Gamal, have a look at chemicon site (www.chemicon.com) and search for hypoxyprobe. You will have staining kit as well good productsheet describing principles of this method and staining procedure. there is good reference list also. It is good to start with...... Jakub ***************************************** Jakub Otahal MD,PhD Department of Developmental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 14220 Prague 4 jotahal@epilepsy.biomed.cas.cz http://epilepsy.biomed.cas.cz tel: +420 241062495 ***************************************** _____ From: Gamal Akabani [mailto:gamal.akabani@gmail.com] To: Histonet ((E-mail)) [mailto:histonet@lists.utsouthwestern.edu] Sent: Mon, 29 Jan 2007 21:40:39 +0100 Subject: [Histonet] Hypoxia Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Tue, 30 Jan 2007 08:37:27 -0500 From: "Houston, Ronald" Subject: [Histonet] RUO's and diagnostic IHC To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77CEFF@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Here is a reply I received yesterday from Dr Steve Gutman, Director, In Vitro Diagnostics, FDA, regarding the current status of using RUOs in the clinical lab. He did add the disclaimer that these were his views and did not represent official FDA policy; and you thought it was confusing before!!!!! Don't shoot the messenger......... "-----Original Message----- From: Gutman, Steve [mailto:steve.gutman@fda.hhs.gov] Sent: Monday, January 29, 2007 2:52 PM To: Houston, Ronald Subject: RE: RUO's and ASR reagents in diagnostics - FDA disclaimer I have had informal discussions with CAP but FDA has not taken a formal position on use of RUOs to support home brews. In general, we think this is a dreadful choice and clearly a tactic of last resort. There are no regulatory requirements for RUO devices. So the laboratory takes full responsibility for ensuring quality of production of the RUO material being made over time. This seems to me to put laboratories at considerable liability risk since the general controls applied to ASRs don't work here. As we try to review our regulatory policy in this area, it is certainly one we should and will try to clarify. Thanks. Steve ________________________________ From: Houston, Ronald [mailto:HoustonR@chi.osu.edu] Sent: Monday, January 29, 2007 10:23 AM To: Gutman, Steve Subject: RUO's and ASR reagents in diagnostics - FDA disclaimer Importance: High Dr Gutman, There is tremendous confusion in laboratory circles regarding the FDA disclaimer for ASR antibodies and whether or not this same disclaimer can be used for RUO antibodies, particularly in light of CAP's change in interpretation of the guidelines for IHC testing: "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew test only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director. The laboratory must establish or verify the performance characteristics of tests using Class I ASR's and RUO's in accordance with the Method Performance Specifications section of the Laboratory General checklist." Exactly what is the current stance of the FDA, and have any discussions taken place between the FDA and CAP regarding their interpretation? Can results of RUO testing be dictated into a diagnostic report and can the test be billed? Obviously, antibody manufacturers mandate that their RUO antibodies cannot be used for diagnostic purposes, but they are as confused as the laboratorians. Thank you for taking the time to clarify this very confusing situation. Sincerely Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu " Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 26 Date: Tue, 30 Jan 2007 07:02:53 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Processing debate To: GMartin@marshallhospital.org, histonet@lists.utsouthwestern.edu Message-ID: <189401.17374.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Fear the word "random", in histology is almost synonym with "caos".There is absolutely no logic or benefit from placing the cassettes at random. If you use a cassetting log the sequencial and orderly manner of placing the cassettes following the log order is the way of doing things. You can always know where your cassettes are and at the embedding step you can follow the same sequency and easily determine if all the cassettes were processed and embeded. It is absolutely illogical to place anything at random (unless you are selecting something for an experimental design that requires that type of selection!). The only "logical" explanation to your initial question is that it stems from the pathologist's lazyness that prefers to throw the cassettes in the container and letting the histotech to try to organize what should not have been disorganized in the first place. Ren? J. GMartin@marshallhospital.org wrote: We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 38, Issue 46 **************************************** Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From thaas <@t> mhhcc.org Tue Jan 30 08:54:41 2007 From: thaas <@t> mhhcc.org (thaas@mhhcc.org) Date: Tue Jan 30 09:55:10 2007 Subject: [Histonet] PLEASE DELETE FROM YOUR LIST In-Reply-To: <45BDF985020000EE00010CF6@smtp-gw.hurleymc.com> Message-ID: PLEASE DELETE FROM HISTONET THANK YOU This e-mail is for the use of the intended recipient(s) only. The information contained in this communication may be confidential, privileged, or protected by copyright, and may be subject to confidentiality agreements. If you are the intended recipient and you do not wish to receive similar electronic messages from us in future then please respond to the sender to this effect. If you have received this email in error, please notify the sender immediately and then delete it. If you are not the intended recipient, you must not keep, use, disclose, copy or distribute this email without the author's prior permission. From MDiCarlo <@t> KaleidaHealth.Org Tue Jan 30 09:58:00 2007 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Tue Jan 30 09:58:16 2007 Subject: [Histonet] bone softening Message-ID: <9B4A77DF11463E4FB723D484214AE9BC02517535@KALEXMB02.KaleidaHealth.org> Histonetters, A while ago I remember someone mentioning a method to soften bone after it's been decaled but prior to processing. Can anyone tell me the procedure and does it have any affect on the H&E staining? Lately, my 5 x 7" size bones have been giving me great difficulty when sectioning since they have been very dense and hard. I process with xylene. Thanks for your help. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From akemiat3377 <@t> yahoo.com Tue Jan 30 10:01:37 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Jan 30 10:01:46 2007 Subject: [Histonet] Processing debate In-Reply-To: <189401.17374.qm@web61213.mail.yahoo.com> Message-ID: <572092.59442.qm@web31305.mail.mud.yahoo.com> I totally agree with Rene. Years ago, we used Technicon processors, the mono, duo & ultra. They were certainly the best thing since sliced bread for those times. I can remember the old Lipshaw too! That took alot of muscle pulling & pushing the baskets back to the formalin station! We would have to empty the cassettes from the formalin container the pathologist placed them into and line them up in numerical order. This was done to ensure the priority bx's came out 1st and that all the following cases were together and placed into the basket in order of importance. This took time at the end of the day, but it saved a considerable amount of time in the morning trying to line all the cases together. Then they color coded the cassettes to distinguish the type of cases. Those color coded cassettes & baskets were a God's send. The new equipment processes tissue so efficiently with heat & vacuum, you don't have to worry about the flow of solutions penetrating the tissues. Unless of course, they are so thick they are squeezed into the cassettes. Even a Divine spirit couldn't help that. Although, the new cassettes that Sakura has developed, puts the ball back into the PA or pathologists court. There is enough "Chaos" in this world! I feel there is no place for it in the laboratory environment. Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com Rene J Buesa wrote: Fear the word "random", in histology is almost synonym with "caos".There is absolutely no logic or benefit from placing the cassettes at random. If you use a cassetting log the sequencial and orderly manner of placing the cassettes following the log order is the way of doing things. You can always know where your cassettes are and at the embedding step you can follow the same sequency and easily determine if all the cassettes were processed and embeded. It is absolutely illogical to place anything at random (unless you are selecting something for an experimental design that requires that type of selection!). The only "logical" explanation to your initial question is that it stems from the pathologist's lazyness that prefers to throw the cassettes in the container and letting the histotech to try to organize what should not have been disorganized in the first place. Ren? J. GMartin@marshallhospital.org wrote: We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Tue Jan 30 10:09:54 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jan 30 10:10:03 2007 Subject: [Histonet] Processing debate In-Reply-To: <189401.17374.qm@web61213.mail.yahoo.com> References: <189401.17374.qm@web61213.mail.yahoo.com> Message-ID: <000a01c74489$1437fb90$db01a8c0@plab.local> We place our cassettes in numerical order. We also use s different color cassette for every 20 cases. Seemed silly when I first got here, Now I rely on those colors to guide me to what comes next. We also use a certain color cassette for STATS and ASAP cases. It's like a" heads up people!" this case needs extra attention. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 30, 2007 9:03 AM To: GMartin@marshallhospital.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processing debate Fear the word "random", in histology is almost synonym with "caos".There is absolutely no logic or benefit from placing the cassettes at random. If you use a cassetting log the sequencial and orderly manner of placing the cassettes following the log order is the way of doing things. You can always know where your cassettes are and at the embedding step you can follow the same sequency and easily determine if all the cassettes were processed and embeded. It is absolutely illogical to place anything at random (unless you are selecting something for an experimental design that requires that type of selection!). The only "logical" explanation to your initial question is that it stems from the pathologist's lazyness that prefers to throw the cassettes in the container and letting the histotech to try to organize what should not have been disorganized in the first place. Ren? J. GMartin@marshallhospital.org wrote: We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Tue Jan 30 10:12:01 2007 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Jan 30 10:12:11 2007 Subject: [Histonet] Processing debate Message-ID: <820734.77376.qm@web30401.mail.mud.yahoo.com> We put the cassettes in order in the baskets right away. I think the most important thing is that the formalin be clean and of significant volume so that it is not diluted by tissues in the cassettes, especially if many contain large sections of fresh tissues. An occasional shake of the basket will probably do the same as the random toss in the bucket. The same goes for fixing large bloody specimens. A change of fresh formalin after the specimen has been in fixative for a few hours will improve fixation. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From ree3 <@t> leicester.ac.uk Tue Jan 30 10:17:50 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Jan 30 10:18:08 2007 Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? In-Reply-To: <995782.58481.qm@web50313.mail.yahoo.com> References: <995782.58481.qm@web50313.mail.yahoo.com> Message-ID: the noun "neat" is a ye olde word for cow, bull or heifer, a "neatherd" would look after them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: 30 January 2007 15:35 To: Christina Thurby; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hi Christina, Yes - "neat" means undiluted. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Christina Thurby To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 4:20:56 PM Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ ____________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgibbon <@t> qltinc.com Tue Jan 30 10:24:19 2007 From: kgibbon <@t> qltinc.com (Kevin Gibbon) Date: Tue Jan 30 10:24:35 2007 Subject: [SPAM] - RE: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? - Email has different SMTP TO: and MIME TO: fields in the email addresses Message-ID: <66A24278845C594C98E5ECB840D7BFB503E17484@VAN-EXCH-VS1.qltinc.com> Presumeably where neatsfoot oil comes from :) kevin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, January 30, 2007 8:18 AM To: Kim Merriam; Christina Thurby; histonet@lists.utsouthwestern.edu Subject: [SPAM] - RE: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? - Email has different SMTP TO: and MIME TO: fields in the email addresses the noun "neat" is a ye olde word for cow, bull or heifer, a "neatherd" would look after them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: 30 January 2007 15:35 To: Christina Thurby; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hi Christina, Yes - "neat" means undiluted. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Christina Thurby To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 4:20:56 PM Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ ____________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. From thaas <@t> mhhcc.org Tue Jan 30 09:39:24 2007 From: thaas <@t> mhhcc.org (thaas@mhhcc.org) Date: Tue Jan 30 10:39:49 2007 Subject: [Histonet] UNSUBSCRIBE Message-ID: Tina Haas, HT Pathology Department Memorial Hospital and Health Care Center Jasper, IN 47546 Ph#812-482-0291 Fax#812-482-0447 thaas@mhhcc.org This e-mail is for the use of the intended recipient(s) only. The information contained in this communication may be confidential, privileged, or protected by copyright, and may be subject to confidentiality agreements. If you are the intended recipient and you do not wish to receive similar electronic messages from us in future then please respond to the sender to this effect. If you have received this email in error, please notify the sender immediately and then delete it. If you are not the intended recipient, you must not keep, use, disclose, copy or distribute this email without the author's prior permission. From billingconsultants <@t> yahoo.com Tue Jan 30 10:42:27 2007 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Tue Jan 30 10:42:37 2007 Subject: [Histonet] Used Microscopes: Attn Vendors Message-ID: <715316.38303.qm@web54213.mail.yahoo.com> Hi, We have a client interested in purchasing a used microscope with a miller disc. If you have any used scopes available, please fax your quotes to 706-546-6522. Thanks so much. Louri Roberts Billing Consultants, LLC www.billingconsultants.net histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Xylene substitute (sheila adey) 2. Re: Xylene substitute (Lynette Pavelich) 3. RE: pens (Bonner, Janet) 4. Miller's Elastic Stain (Andrea Grantham) 5. Hypoxia (Gamal Akabani) 6. RE: Miller's Elastic Stain (Monfils, Paul) 7. If you are even remotely curious, please call! Even more temp openings-- (Cheryl Kerry) 8. Re: Miller's Elastic Stain (Bryan Hewlett) 9. Re: Miller's Elastic Stain (Bryan Llewellyn) 10. Re: Miller's Elastic Stain (Andrea Grantham) 11. Re: Anybody doing flow cytometry? What does Neat mean? (Christina Thurby) 12. ASCP Exam (Stasha McCollum) 13. Processing debate (GMartin@marshallhospital.org) 14. a question (nizar limaiem) 15. Re: ASCP Exam (Kaye Ryan) 16. human total IgG (Liz Chlipala) 17. RE: Re: Anybody doing flow cytometry? What does Neat mean? (Monfils, Paul) 18. MCP1 positive control (Brown, Graham W) 19. Re: Mouse Testes Fixation and Processing (Laurie Reilly) 20. Re: Immunogold question (Robert Chiovetti) 21. Follow up (GMartin@marshallhospital.org) 22. RE: Re: Anybody doing flow cytometry? What does Neat mean? (Tarango, Mark) 23. ASCP EXAM (MICHELLE SEAGLE) 24. Re: Hypoxia (Jakub Otahal) 25. RUO's and diagnostic IHC (Houston, Ronald) 26. Re: Processing debate (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Jan 2007 13:38:44 -0500 From: "sheila adey" Subject: RE: [Histonet] Xylene substitute To: GMartin@marshallhospital.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed We tried using formula 88 and found it to be also irritating, especially after a spill. It was a problem when we tried to coverslip because our sugipath mounting media is Xylene based but since you have already covered that issue, it would be nice to hear what you think of the quality of the product. Please share after you've been using it. Sheila Adey HT MLT Port Huron Hospital Michigan >From: GMartin@marshallhospital.org >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Xylene substitute >Date: Mon, 29 Jan 2007 09:15:00 -0800 > >We presently use Xylene in our processor. We will be moving to a Xylene >substitute. We are a small lab and still hand cover all of our slide. In >the cover slip line we do use Sugipath's Sub-x Xylene substitute and are >planning on using Sub-x. >Can anyone in the group fill me in on their experience with a change over >to a Xylene substitute. >Thank you >Gary Martin >El Dorado pathology >California. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA ------------------------------ Message: 2 Date: Mon, 29 Jan 2007 13:41:25 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Xylene substitute To: , Message-ID: <45BDF985020000EE00010CF6@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Hi Gary, We recenty had a change over to a substitute and went with the aliphatic Propar by Anatech. Easy transition, very low oder, docs didn't notice (big plus). We did add an extra container on the stainer when depariffinizing, and on the processor, we have 3 containers of the substitute. We are also able to recycle it. Works well with hand coverslipping media also. You'll be happy with the change. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> 01/29/07 12:15 PM >>> We presently use Xylene in our processor. We will be moving to a Xylene substitute. We are a small lab and still hand cover all of our slide. In the cover slip line we do use Sugipath's Sub-x Xylene substitute and are planning on using Sub-x. Can anyone in the group fill me in on their experience with a change over to a Xylene substitute. Thank you Gary Martin El Dorado pathology California. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Jan 2007 14:51:14 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] pens To: "Patricia Karlisch" , Histonet@lists.utsouthwestern.edu, "ruegg, patsy" , "Webb', 'Dorothy L" Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A73C@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 We have been looking for a similar pen that will stay on the cassettes AND the slides. Right now we use two different pens. Smudging has been a problem, but the Mercedes pens seem like a winner! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patricia Karlisch Sent: Mon 1/29/2007 9:18 AM To: Histonet@lists.utsouthwestern.edu; ruegg, patsy; Webb', 'Dorothy L Subject: RE: [Histonet] pens All, I have received good recommendations for cassette pens and it looks like there are many more pens out there then I knew about. We will try each one and see if this alleviates the smudging. Thank you all so much. Pat Karlisch. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "patsy ruegg" 1/27/2007 7:44 PM >>> We use pens from StatLabs we like, just have to let the cassettes dry a minute or two before putting them in alcohol waiting for processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 8:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 4 Date: Mon, 29 Jan 2007 13:33:12 -0700 From: Andrea Grantham Subject: [Histonet] Miller's Elastic Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Happy Monday! I have had a request to do a Miller's Elastic Stain. I have almost all of the stains and I do have all of the chemicals to do the stain but I have a question. One of the ingredients in the elastic stain is resorcin. I'm not a chemist so I have to ask this question, I have resorcinol in my chemical cabinet - is this the same thing as resorcin? I can find resorcinol in the Sigma cat. but not resorcin. Thanks! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 5 Date: Mon, 29 Jan 2007 14:40:39 -0600 From: Gamal Akabani Subject: [Histonet] Hypoxia To: "Histonet ((E-mail))" Message-ID: <4885123C-E70A-41BC-AAE0-1F483B088D7A@gmail.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal ------------------------------ Message: 6 Date: Mon, 29 Jan 2007 15:50:40 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Miller's Elastic Stain To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C0E@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Yes, resorcinol and resorcin are the same thing, C6H6O2 ------------------------------ Message: 7 Date: Mon, 29 Jan 2007 14:53:02 -0600 From: "Cheryl Kerry" Subject: [Histonet] If you are even remotely curious, please call! Even more temp openings-- To: Message-ID: <00b501c743e7$77b140c0$6401a8c0@FSDESKTOP> Content-Type: text/plain; charset="US-ASCII" Hi All! We're swamped and need more great techs!! If you have ever been ever remotely curious about travel temps, I would love to talk with you. Even if you won't be ready for a year or more--let's start the conversation so when you are ready to go, you're comfortable and happy in your decision. I keep in touch. I don't pressure you (it is YOUR life, right?) Someone said I don't come across as a used car salesman.I'm a histotech!! If something doesn't fit--you tell me 'no' and we move on to the next opening. Easy as that. I have EIGHT open positions and need to fill them within the next two weeks. All shifts, all skills. With the changes in the registry and state licensing, we're going to continue to need great techs who want to try new things. Give me a call--it's an investment of about 15 minutes for the basics. I help new travelers get started and we've got a great team. It all starts with a question- "So, tell me about temping?" I'll give you the details of the jobs that fit when we get a chance to chat!! Cheryl :-) Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 800.756.3309 ------------------------------ Message: 8 Date: Mon, 29 Jan 2007 15:54:58 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] Miller's Elastic Stain To: , "Andrea Grantham" Message-ID: <000d01c743e7$bd2f0470$6500a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Andi, Resorcin is the same thing as resorcinol! Bryan ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 3:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Mon, 29 Jan 2007 12:43:26 -0800 From: Bryan Llewellyn Subject: Re: [Histonet] Miller's Elastic Stain To: Histonet , Andrea Grantham Message-ID: <001201c743e6$209f0520$6400a8c0@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=response Same thing. Bryan Llewellyn ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 12:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 29 Jan 2007 14:19:22 -0700 From: Andrea Grantham Subject: Re: [Histonet] Miller's Elastic Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20070129141822.00e1a5e0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Thanks! Glad to hear that it is the same thing. Looks like I'm good to go. Andi At 01:33 PM 1/29/2007 -0700, Andrea Grantham wrote: >Happy Monday! >I have had a request to do a Miller's Elastic Stain. I have almost all of >the stains and I do have all of the chemicals to do the stain but I have a >question. >One of the ingredients in the elastic stain is resorcin. I'm not a chemist >so I have to ask this question, I have resorcinol in my chemical cabinet - >is this the same thing as resorcin? I can find resorcinol in the Sigma >cat. but not resorcin. > >Thanks! >Andi Grantham >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : === message truncated === --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From SHargrove <@t> urhcs.org Tue Jan 30 11:39:20 2007 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Tue Jan 30 11:39:32 2007 Subject: Subject: [Histonet] Processing debate In-Reply-To: <20070130164923.B50431C8003@zixvpm01.urhcs.org> Message-ID: We currently run 2 VIP processors with different programs. The cassettes are put in the basket, angle up, as they are grossed. Most of the time in a fairly decent order. The basket is in formalin. At the end of the day we can just pull out the ones that go on a separate run and then leave the other ones in order. If you have blocks "floating" in formalin, I would worry about fixation. The only block I see floating are the ones that the person grossing uses dry sponges in. Susie Hargrove Tech Specialist, Histology United Regional Health Care Systems. Wichita Falls, Texas ********************************************************************** The documents inside this electronic transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled, unless otherwise required by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you received this electronic transmission in error, please notify the sender immediately to arrange for return. ********************************************************************** From KMENDELL <@t> nhs-healthlink.org Tue Jan 30 12:21:24 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Tue Jan 30 12:21:49 2007 Subject: [Histonet] Does anyone know where or if Black Warrior #2 pencils can still be bought? If so where? Message-ID: <45BF4654020000A800010345@mail.NHS-HEALTHLINK.ORG> Does anyone know where or if Black Warrior #2 pencils can still be bought? If so where? ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From harrisonburgcytology <@t> yahoo.com Tue Jan 30 12:51:44 2007 From: harrisonburgcytology <@t> yahoo.com (Shirley Martin) Date: Tue Jan 30 12:51:52 2007 Subject: [Histonet] HPV Final Report Using Ventana Message-ID: <942893.88257.qm@web36811.mail.mud.yahoo.com> Hello All, I was wondering if anyone knew what kind of diagnosis wording would work best for reporting HPV results from the Ventana system? The Ventana guys also mentioned a disclaimer that should be included on the final report due to its status as an ASR. Any help would be greatly appreciated. Thanks, Tom Ward M.S. CT(ASCP) Harrisonburg Cytology Services harrisonburgcytology@yahoo.com (540) 289-7558 --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. From Melissa.Zummak <@t> ssfhs.org Tue Jan 30 13:27:07 2007 From: Melissa.Zummak <@t> ssfhs.org (Zummak Melissa) Date: Tue Jan 30 13:27:25 2007 Subject: [Histonet] HPV Final Report Using Ventana Message-ID: I have been using this method for about 3 years now and our reports read for diagnosis: The result for HPV testing by in-situ hybridization for this patient was (either "detected",or "not detected", or we also use "determined equivocal"). The CAP checklist has some suggestions for the disclaimer, we use "This test was developed and its performance characteristics determined by (your lab name). It has not been cleared or approved by the U.S. FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is regulated under CLIA 88 as qualified to perform high complexity clinical testing. Melissa Zummak -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Martin Sent: Tuesday, January 30, 2007 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPV Final Report Using Ventana Hello All, I was wondering if anyone knew what kind of diagnosis wording would work best for reporting HPV results from the Ventana system? The Ventana guys also mentioned a disclaimer that should be included on the final report due to its status as an ASR. Any help would be greatly appreciated. Thanks, Tom Ward M.S. CT(ASCP) Harrisonburg Cytology Services harrisonburgcytology@yahoo.com (540) 289-7558 --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From heyitsra <@t> gmail.com Tue Jan 30 14:09:29 2007 From: heyitsra <@t> gmail.com (Ra) Date: Tue Jan 30 14:09:38 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: <831462.68750.qm@web51812.mail.yahoo.com> References: <831462.68750.qm@web51812.mail.yahoo.com> Message-ID: <358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > From jqb7 <@t> cdc.gov Tue Jan 30 14:21:41 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Jan 30 14:22:00 2007 Subject: [Histonet] ASCP EXAM References: <831462.68750.qm@web51812.mail.yahoo.com> <358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> Message-ID: Are we discussing the HT or the HTL? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra Sent: Tue 1/30/2007 3:09 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] ASCP EXAM Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Tue Jan 30 16:01:04 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Jan 30 16:01:25 2007 Subject: [Histonet] IHC and/or ISH submission worksheets Message-ID: Hi all, I'm currently working on an inhouse LIMS (laboratory information management system) project and I'd like to incorporate a worksheet as part of the electronic sample submission process for IHC and one for ISH requests. If any of you currently have a model you are using and don't mind sharing, I would be most grateful. Additionally, I would also appreciate any ideas, suggestions, and pointers you can throw my way. Our LIMS will run in a browser, in case you were wondering. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From AGrobe2555 <@t> aol.com Tue Jan 30 16:12:15 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Jan 30 16:12:34 2007 Subject: [Histonet] Sheep eNOS IHC Message-ID: Has anyone had any luck doing eNOS IHC on FFPE sheep tissues? If so, would you mind sharing your Ab source and protocol. Thanks, Albert From sterntaler3 <@t> hotmail.com Tue Jan 30 18:36:18 2007 From: sterntaler3 <@t> hotmail.com (gloria @home) Date: Tue Jan 30 18:36:33 2007 Subject: [Histonet] PLEASE DELETE FROM YOUR LIST In-Reply-To: Message-ID: Please delete me too thanks >From: thaas@mhhcc.org >To: >Subject: [Histonet] PLEASE DELETE FROM YOUR LIST >Date: Tue, 30 Jan 2007 09:54:41 -0500 > >PLEASE DELETE FROM HISTONET THANK YOU > > > > > >This e-mail is for the use of the intended recipient(s) only. The >information contained in this communication may be confidential, >privileged, or protected by copyright, and may be subject to >confidentiality agreements. If you are the intended recipient and you do >not wish to receive similar electronic messages from us in future then >please respond to the sender to this effect. If you have received this >email in error, please notify the sender immediately and then delete it. >If you are not the intended recipient, you must not keep, use, disclose, >copy or distribute this email without the author's prior permission. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Find singles online in your area with MSN Dating and Match.com! http://cp.intl.match.com/eng/msn/msnsg/wbc/wbc.html From luke.perkocha <@t> ucsf.edu Tue Jan 30 18:45:07 2007 From: luke.perkocha <@t> ucsf.edu (Luke Perkocha) Date: Tue Jan 30 18:45:37 2007 Subject: [Histonet] Digital Dictation System Message-ID: <6.2.3.4.2.20070130163924.025518e0@exchange.ucsf.edu> We are looking to replace an aging digital dictation system for gross and micro pathology with something that is easy and intuitive to manage and flexible, so dictation can be done from a dedicated unit (plugged into a phone line - no cabling) with a foot pedal, or by dial-in from an outside phone. We also need to be able to manage transcription, send dictations around and select specific dictations for transcription all from multiple sites for both dictation and transcription. It would be nice if the product can be adapted for voice recognition in the future, but we're not planning to do that soon. I googled "digital dictation" and there seem to be a lot of products out there widely ranging in price from high end "enterprise" systems to software in a box. Does anyone have a good solution to recommend, or can you suggest a consultant who can figure this out? Thanks, please copy your response to my e-mail: luke@wpcreno.com Luke From jnocito <@t> satx.rr.com Tue Jan 30 20:55:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jan 30 20:54:55 2007 Subject: [Histonet] HPV Final Report Using Ventana References: Message-ID: <00b501c744e3$3cbc0b10$649eae18@yourxhtr8hvc4p> Shirley, contact Ventana and tell them you want the "white sheets" for the ISH. These have specific results listed. I used these for my procedures and the CAP inspector accepted those results. I was inspected on the 19th. I received my sheets free, but then again :-(> Joe ----- Original Message ----- From: "Zummak Melissa" To: "Shirley Martin" ; Sent: Tuesday, January 30, 2007 1:27 PM Subject: RE: [Histonet] HPV Final Report Using Ventana I have been using this method for about 3 years now and our reports read for diagnosis: The result for HPV testing by in-situ hybridization for this patient was (either "detected",or "not detected", or we also use "determined equivocal"). The CAP checklist has some suggestions for the disclaimer, we use "This test was developed and its performance characteristics determined by (your lab name). It has not been cleared or approved by the U.S. FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is regulated under CLIA 88 as qualified to perform high complexity clinical testing. Melissa Zummak -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Martin Sent: Tuesday, January 30, 2007 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPV Final Report Using Ventana Hello All, I was wondering if anyone knew what kind of diagnosis wording would work best for reporting HPV results from the Ventana system? The Ventana guys also mentioned a disclaimer that should be included on the final report due to its status as an ASR. Any help would be greatly appreciated. Thanks, Tom Ward M.S. CT(ASCP) Harrisonburg Cytology Services harrisonburgcytology@yahoo.com (540) 289-7558 --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Wed Jan 31 02:15:45 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed Jan 31 02:16:13 2007 Subject: [Histonet] RE: Immunogold question Message-ID: <32910d325691.32569132910d@amc.uva.nl> Hi Danielle and Robert, Indeed, Robert is right with his response: the higher pH helps in reducing background staining. It has something to do with the charges introduced by the gold particles. At higher pH they are near neutral, at lower pH the gold particles are more negatively charged causing non-specific binding with the positively charged tissue elements (especially after aldehyde fixation). Changing the pH is certainly not the only thing you need to do to suppress background staining. Additives like BSA-c and Cold Water Fish Skin Gelatin applied in the right incubation step also contributes to a cleaner image. For the experts on this issue go to: [1]www.aurion.nl (English website). Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands From: Danielle Crippen To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 10:27:46 AM Subject: [Histonet] Immunogold question Dear EM experts, I've been using the following protocol for immunogold labeling for the past couple years with good success. This morning one of our users has inquired as to why the secondary is incubated at a higher pH than the primary. I do not know the answer...do any of you??? References 1. http://www.aurion.nl/ From BMolinari <@t> heart.thi.tmc.edu Wed Jan 31 05:34:51 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Jan 31 05:34:57 2007 Subject: [Histonet] RE:xylene substitute and marking pens In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49103C32139@thm-mail.thomaswv.org> Message-ID: I switched to Formula 83 a few months ago because of xylene fume complaints. I have been very pleased as well. I also us RA coverslipping medium. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Price, Tiffany Sent: Tuesday, January 30, 2007 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:xylene substitute and marking pens We use the Formula 83 substitute and really like it. It also recycles very well. We use Richard Allen mounting medium to coverslip- it is the most compatible, and recommended by CBG Biotech, the manufacturer of the clearant. We also use SHUR/MARK pens from Triangle Biomedical Sciences- they have a refillable tip that you just replace until the ink runs out of the pen. They come in red or black. I have found that these are the only pens that work with the Formula 83 clearant. Hope this helps! Tiffany -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 30, 2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 38, Issue 46 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Xylene substitute (sheila adey) 2. Re: Xylene substitute (Lynette Pavelich) 3. RE: pens (Bonner, Janet) 4. Miller's Elastic Stain (Andrea Grantham) 5. Hypoxia (Gamal Akabani) 6. RE: Miller's Elastic Stain (Monfils, Paul) 7. If you are even remotely curious, please call! Even more temp openings-- (Cheryl Kerry) 8. Re: Miller's Elastic Stain (Bryan Hewlett) 9. Re: Miller's Elastic Stain (Bryan Llewellyn) 10. Re: Miller's Elastic Stain (Andrea Grantham) 11. Re: Anybody doing flow cytometry? What does Neat mean? (Christina Thurby) 12. ASCP Exam (Stasha McCollum) 13. Processing debate (GMartin@marshallhospital.org) 14. a question (nizar limaiem) 15. Re: ASCP Exam (Kaye Ryan) 16. human total IgG (Liz Chlipala) 17. RE: Re: Anybody doing flow cytometry? What does Neat mean? (Monfils, Paul) 18. MCP1 positive control (Brown, Graham W) 19. Re: Mouse Testes Fixation and Processing (Laurie Reilly) 20. Re: Immunogold question (Robert Chiovetti) 21. Follow up (GMartin@marshallhospital.org) 22. RE: Re: Anybody doing flow cytometry? What does Neat mean? (Tarango, Mark) 23. ASCP EXAM (MICHELLE SEAGLE) 24. Re: Hypoxia (Jakub Otahal) 25. RUO's and diagnostic IHC (Houston, Ronald) 26. Re: Processing debate (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Jan 2007 13:38:44 -0500 From: "sheila adey" Subject: RE: [Histonet] Xylene substitute To: GMartin@marshallhospital.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed We tried using formula 88 and found it to be also irritating, especially after a spill. It was a problem when we tried to coverslip because our sugipath mounting media is Xylene based but since you have already covered that issue, it would be nice to hear what you think of the quality of the product. Please share after you've been using it. Sheila Adey HT MLT Port Huron Hospital Michigan >From: GMartin@marshallhospital.org >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Xylene substitute >Date: Mon, 29 Jan 2007 09:15:00 -0800 > >We presently use Xylene in our processor. We will be moving to a Xylene >substitute. We are a small lab and still hand cover all of our slide. In >the cover slip line we do use Sugipath's Sub-x Xylene substitute and are >planning on using Sub-x. >Can anyone in the group fill me in on their experience with a change over >to a Xylene substitute. >Thank you >Gary Martin >El Dorado pathology >California. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA ------------------------------ Message: 2 Date: Mon, 29 Jan 2007 13:41:25 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Xylene substitute To: , Message-ID: <45BDF985020000EE00010CF6@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Hi Gary, We recenty had a change over to a substitute and went with the aliphatic Propar by Anatech. Easy transition, very low oder, docs didn't notice (big plus). We did add an extra container on the stainer when depariffinizing, and on the processor, we have 3 containers of the substitute. We are also able to recycle it. Works well with hand coverslipping media also. You'll be happy with the change. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> 01/29/07 12:15 PM >>> We presently use Xylene in our processor. We will be moving to a Xylene substitute. We are a small lab and still hand cover all of our slide. In the cover slip line we do use Sugipath's Sub-x Xylene substitute and are planning on using Sub-x. Can anyone in the group fill me in on their experience with a change over to a Xylene substitute. Thank you Gary Martin El Dorado pathology California. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Jan 2007 14:51:14 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] pens To: "Patricia Karlisch" , Histonet@lists.utsouthwestern.edu, "ruegg, patsy" , "Webb', 'Dorothy L" Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A73C@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 We have been looking for a similar pen that will stay on the cassettes AND the slides. Right now we use two different pens. Smudging has been a problem, but the Mercedes pens seem like a winner! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patricia Karlisch Sent: Mon 1/29/2007 9:18 AM To: Histonet@lists.utsouthwestern.edu; ruegg, patsy; Webb', 'Dorothy L Subject: RE: [Histonet] pens All, I have received good recommendations for cassette pens and it looks like there are many more pens out there then I knew about. We will try each one and see if this alleviates the smudging. Thank you all so much. Pat Karlisch. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "patsy ruegg" 1/27/2007 7:44 PM >>> We use pens from StatLabs we like, just have to let the cassettes dry a minute or two before putting them in alcohol waiting for processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Wednesday, January 24, 2007 8:54 AM To: Dorothy L Webb; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pens Dorothy, Thank you for this information. We are looking for non-smudge pens as well. This will help. Did you find that the Surgipath pens did not smudge or come off during processing? Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Webb, Dorothy L" 1/24/2007 10:49 AM >>> We recently had the same problems and researched several companies and brands and found for our usage the following were the best: Surgipath pens #01880 come in a box of 10 for $27.00 Marketlab pens #ML0479 come in a box of 10 for $37.00 Mercedes Medical has a new pen from Norway that we tried that are only $13 per box of 10, but do not have the order number for them as they are not in stock quite yet. The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did not smear, etc., but dried out so fast and often came already dry in the box. Good Luck!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 4 Date: Mon, 29 Jan 2007 13:33:12 -0700 From: Andrea Grantham Subject: [Histonet] Miller's Elastic Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20070129130923.00e1fec0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Happy Monday! I have had a request to do a Miller's Elastic Stain. I have almost all of the stains and I do have all of the chemicals to do the stain but I have a question. One of the ingredients in the elastic stain is resorcin. I'm not a chemist so I have to ask this question, I have resorcinol in my chemical cabinet - is this the same thing as resorcin? I can find resorcinol in the Sigma cat. but not resorcin. Thanks! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 5 Date: Mon, 29 Jan 2007 14:40:39 -0600 From: Gamal Akabani Subject: [Histonet] Hypoxia To: "Histonet ((E-mail))" Message-ID: <4885123C-E70A-41BC-AAE0-1F483B088D7A@gmail.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal ------------------------------ Message: 6 Date: Mon, 29 Jan 2007 15:50:40 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Miller's Elastic Stain To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C0E@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Yes, resorcinol and resorcin are the same thing, C6H6O2 ------------------------------ Message: 7 Date: Mon, 29 Jan 2007 14:53:02 -0600 From: "Cheryl Kerry" Subject: [Histonet] If you are even remotely curious, please call! Even more temp openings-- To: Message-ID: <00b501c743e7$77b140c0$6401a8c0@FSDESKTOP> Content-Type: text/plain; charset="US-ASCII" Hi All! We're swamped and need more great techs!! If you have ever been ever remotely curious about travel temps, I would love to talk with you. Even if you won't be ready for a year or more--let's start the conversation so when you are ready to go, you're comfortable and happy in your decision. I keep in touch. I don't pressure you (it is YOUR life, right?) Someone said I don't come across as a used car salesman.I'm a histotech!! If something doesn't fit--you tell me 'no' and we move on to the next opening. Easy as that. I have EIGHT open positions and need to fill them within the next two weeks. All shifts, all skills. With the changes in the registry and state licensing, we're going to continue to need great techs who want to try new things. Give me a call--it's an investment of about 15 minutes for the basics. I help new travelers get started and we've got a great team. It all starts with a question- "So, tell me about temping?" I'll give you the details of the jobs that fit when we get a chance to chat!! Cheryl :-) Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 800.756.3309 ------------------------------ Message: 8 Date: Mon, 29 Jan 2007 15:54:58 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] Miller's Elastic Stain To: , "Andrea Grantham" Message-ID: <000d01c743e7$bd2f0470$6500a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Andi, Resorcin is the same thing as resorcinol! Bryan ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 3:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Mon, 29 Jan 2007 12:43:26 -0800 From: Bryan Llewellyn Subject: Re: [Histonet] Miller's Elastic Stain To: Histonet , Andrea Grantham Message-ID: <001201c743e6$209f0520$6400a8c0@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=response Same thing. Bryan Llewellyn ----- Original Message ----- From: "Andrea Grantham" To: Sent: Monday, January 29, 2007 12:33 PM Subject: [Histonet] Miller's Elastic Stain > Happy Monday! > I have had a request to do a Miller's Elastic Stain. I have almost all of > the stains and I do have all of the chemicals to do the stain but I have a > question. > One of the ingredients in the elastic stain is resorcin. I'm not a chemist > so I have to ask this question, I have resorcinol in my chemical cabinet - > is this the same thing as resorcin? I can find resorcinol in the Sigma > cat. but not resorcin. > > Thanks! > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 29 Jan 2007 14:19:22 -0700 From: Andrea Grantham Subject: Re: [Histonet] Miller's Elastic Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20070129141822.00e1a5e0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Thanks! Glad to hear that it is the same thing. Looks like I'm good to go. Andi At 01:33 PM 1/29/2007 -0700, Andrea Grantham wrote: >Happy Monday! >I have had a request to do a Miller's Elastic Stain. I have almost all of >the stains and I do have all of the chemicals to do the stain but I have a >question. >One of the ingredients in the elastic stain is resorcin. I'm not a chemist >so I have to ask this question, I have resorcinol in my chemical cabinet - >is this the same thing as resorcin? I can find resorcinol in the Sigma >cat. but not resorcin. > >Thanks! >Andi Grantham >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 11 Date: Mon, 29 Jan 2007 15:20:56 -0600 From: Christina Thurby Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? To: histonet@lists.utsouthwestern.edu Message-ID: <45BE6538.4090202@bms.com> Content-Type: text/plain; charset="iso-8859-1" Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina ------------------------------ Message: 12 Date: Mon, 29 Jan 2007 13:30:17 -0800 (PST) From: Stasha McCollum Subject: [Histonet] ASCP Exam To: histonet@lists.utsouthwestern.edu Message-ID: <20070129213017.85649.qmail@web35009.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Can anyone tell me if the practical portion of the certification exam has been remove? Ther were rumors floating about that change last year at this time. Also if anyone has taken it recently what is the percentage of IHC on the exam Thanks --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. ------------------------------ Message: 13 Date: Mon, 29 Jan 2007 13:44:41 -0800 From: GMartin@marshallhospital.org Subject: [Histonet] Processing debate To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California ------------------------------ Message: 14 Date: Mon, 29 Jan 2007 22:56:26 +0100 From: "nizar limaiem" Subject: [Histonet] a question To: histonet@lists.utsouthwestern.edu Message-ID: <92805bf10701291356j3e107923md8a75f1a0929f8a7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Doctor I am student in biotechnology (Tunisia), I work on the developpement of the Embryo and Early Larva of a fish species dicentrarchus labrax L (it's egg is about 1mm in diameter). I want to use methods of histology on fertilized eggs. Could some one offer some suggestions on the experimental protocol (fixation/processing/sectioning) Thank you for your reply limaiem nizar. Student in marin biotechnology University of biotechnology, Monastir, Tunisise ------------------------------ Message: 15 Date: Mon, 29 Jan 2007 17:08:03 -0500 From: "Kaye Ryan" Subject: Re: [Histonet] ASCP Exam To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Hi, Yes, the practical portion of the exam has been done away with. I had a student that just took the exam. There are many more troubleshooting questions now on all aspects of histology. My recommendations based on what the student told me is that you need to really focus on troubleshooting of special stains as well as fixation and cutting. As far as I can tell, the study guide has not been updated and I don't know if it will be. Good luck, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> Stasha McCollum 01/29/07 4:30 PM >>> Can anyone tell me if the practical portion of the certification exam has been remove? Ther were rumors floating about that change last year at this time. Also if anyone has taken it recently what is the percentage of IHC on the exam Thanks --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 29 Jan 2007 15:36:54 -0700 From: "Liz Chlipala" Subject: [Histonet] human total IgG To: "'Histonet'" Message-ID: <000001c743f5$f9f72280$0d00a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" Hello everyone Does anyone out there know of an antibody that will detect total IgG for human tissue in paraffin sections. I have searched the web and have been unable to come up with one that will detect total IgG so any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 ------------------------------ Message: 17 Date: Mon, 29 Jan 2007 17:29:30 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C11@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Yep, it means the same thing for antibodies that it means for scotch - undiluted - straight from the bottle. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Christina Thurby > Sent: Monday, January 29, 2007 1:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? > > <> > > Hello Histonet Folks!, > Is anyone out there performing flow cytometry that can tell me what a > suggested working dilution "Neat" means. I think it is the use of an > 'undiluted' primary antibody but if someone familiar with this term can > please confirm that I'd be very appreciative. > Thanks, > Christina > > > ------------------------------ Message: 18 Date: Mon, 29 Jan 2007 16:37:31 -0600 From: "Brown, Graham W" Subject: [Histonet] MCP1 positive control To: Message-ID: <7F7DE44DEE269C4CB34BB8EADE0525EC61E472@BOWIE.ttuhsc.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone know of a positive control tissue for MCP1?? thanks Graham Brown Texas Tech University HSC Garrison Institute on Aging Dept. Neurology Lubbock, TX ------------------------------ Message: 19 Date: Tue, 30 Jan 2007 09:19:15 +1000 From: Laurie Reilly Subject: Re: [Histonet] Mouse Testes Fixation and Processing To: djemge@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <5.2.0.9.0.20070130090758.00bea1f0@mail.jcu.edu.au> Content-Type: text/plain; charset="us-ascii"; format=flowed Donna and All, Bouin's is the fixative of choice for testis, and my experience with formalin fixation of testis tells me that your observations are correct. A formalin-acetic acid-ethanol mix may be better. Maybe Davidson's fixative? Another "trick of the trade" is to start the processing of testis (and other very delicate tissues) in 30% Ethanol then 50%, 70%, 80%, 90%, 95%, 100%, 100% then 50:50 EtOH:Xylene, 2x Xylene 3x Paraffin. For mouse tissues 15 minutes in each of these reagents would be enough. Regards, Laurie. At 11:18 AM 29/01/2007 -0500, djemge@aol.com wrote: >Hello All. I am having an ongoing problem with formalin fixed, paraffin >embedded whole mouse testes (Adult mouse and Pups). I need to get this >issue resolved and have consistent results for my investigators. They are >using these testis for xgal, IF, IHC and In Situ. All my Bouin's fixed >testes looks beautiful but the formalin fixed testes are horrible. Bouins >is not suitable for much of what they are doing. I have tried several >processing schedules and nothing seems to work. Sometimes the FFPE's seem >very dry and shrunk, sometimes the cells look like they have big halos >around them and you can't distinguish them enough for staging. This is >frustrating! All other tissue seems to be fine except these and there is a >big variability in how these testes turn out. They are NBF or cold 4%PFA >PBS fixed for 24 hours. Typical processing schedule is either 30 minutes >each station or 1 hour each station (formalin, 2x70%, 80%, 2x95%, 2x100% >reagent alcohol, 2x Xylene, 3x Ameraffin Paraffin! > (Cardinal cat# M7346-1A). > >Donna J. Emge, HT(ASCP) >Northwestern University >Lurie 7-220 >303 E. Superior Avenue >Chicago, IL 60611 >312-503-2036 >d-emge@northwestern.edu >________________________________________________________________________ >Check out the new AOL. Most comprehensive set of free safety and security >tools, free access to millions of high-quality videos from across the web, >free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 ------------------------------ Message: 20 Date: Mon, 29 Jan 2007 15:34:38 -0800 (PST) From: Robert Chiovetti Subject: Re: [Histonet] Immunogold question To: Danielle Crippen , histonet@lists.utsouthwestern.edu Message-ID: <825913.37628.qm@web58903.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Danielle, Just a guess here, but the higher pH may help reduce nonspecific background staining. I worked in an EM lab that occasionally played with pH and salt concentrations for immunogold labeling when it was necessary. I know for sure that high salt washes after the primary and secondary Ab's, followed by a return to normal TBS, does a super job of getting rid of background. I'm not so certain about the pH. I only used it a couple of times and I really couldn't tell much difference... Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Danielle Crippen To: histonet@lists.utsouthwestern.edu Sent: Monday, January 29, 2007 10:27:46 AM Subject: [Histonet] Immunogold question Dear EM experts, I've been using the following protocol for immunogold labeling for the past couple years with good success. This morning one of our users has inquired as to why the secondary is incubated at a higher pH than the primary. I do not know the answer...do any of you??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Want to start your own business? Learn how on Yahoo! Small Business. http://smallbusiness.yahoo.com/r-index ------------------------------ Message: 21 Date: Mon, 29 Jan 2007 16:06:53 -0800 From: GMartin@marshallhospital.org Subject: [Histonet] Follow up To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I'm new to the list and in an effort to keep the list informed I want to thank you for the response to my two questions. 1) concerning Xylene substitutes ... I received a good suggestion on a product FORMULA 83. And I will as suggested ... report back on my success or failures of my transition from Xylene to a substitute. 2) Processing cassettes ... the debate was weather to put them directly into the processing basket or let them float randomly in formalin then put them in the basket. The standard seems to be to load them directly from the grossing table into the processing basket. Thank you all very much Gary Martin El Dorado Pathology ------------------------------ Message: 22 Date: Mon, 29 Jan 2007 16:31:31 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? To: "Christina Thurby" , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F9951@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii You're right. It means using it straight. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christina Thurby Sent: Monday, January 29, 2007 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Anybody doing flow cytometry? What does Neat mean? Hello Histonet Folks!, Is anyone out there performing flow cytometry that can tell me what a suggested working dilution "Neat" means. I think it is the use of an 'undiluted' primary antibody but if someone familiar with this term can please confirm that I'd be very appreciative. Thanks, Christina "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 23 Date: Mon, 29 Jan 2007 18:45:11 -0800 (PST) From: MICHELLE SEAGLE Subject: [Histonet] ASCP EXAM To: histonet@lists.utsouthwestern.edu Message-ID: <831462.68750.qm@web51812.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I just took HT exam this past december and I dont remember having any IHC related questions on the exam. You just need to know the freida carson book backwards and forwards because they pick the starngest things out of that book that you would not know unless you have just practically memorized the book!! Don't wast your time or money on the ASCP practical exam questions, what a waste. As far as the practical part I am not sure but I believe It has been Removed from the requirements since everthing is so automated now. Good Luck on your Exam Michelle Seagle HT (ASCP) Rutherford Hospital --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. ------------------------------ Message: 24 Date: Tue, 30 Jan 2007 06:53:19 +0100 From: "Jakub Otahal" Subject: Re: [Histonet] Hypoxia To: histonet@lists.utsouthwestern.edu Message-ID: <20070130055319.9dbd9a05@epilepsy.biomed.cas.cz> Content-Type: text/plain; charset="us-ascii" Dear Gamal, have a look at chemicon site (www.chemicon.com) and search for hypoxyprobe. You will have staining kit as well good productsheet describing principles of this method and staining procedure. there is good reference list also. It is good to start with...... Jakub ***************************************** Jakub Otahal MD,PhD Department of Developmental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 14220 Prague 4 jotahal@epilepsy.biomed.cas.cz http://epilepsy.biomed.cas.cz tel: +420 241062495 ***************************************** _____ From: Gamal Akabani [mailto:gamal.akabani@gmail.com] To: Histonet ((E-mail)) [mailto:histonet@lists.utsouthwestern.edu] Sent: Mon, 29 Jan 2007 21:40:39 +0100 Subject: [Histonet] Hypoxia Dear friends, I am want to process some histological samples from glioma patients in order to observe the level or grade of hypoxia on them, if any. I am not a guru. Any help is appreciated on the methods or a specific antibody used to visualize it. Thanks in advanced. Gamal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Tue, 30 Jan 2007 08:37:27 -0500 From: "Houston, Ronald" Subject: [Histonet] RUO's and diagnostic IHC To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77CEFF@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Here is a reply I received yesterday from Dr Steve Gutman, Director, In Vitro Diagnostics, FDA, regarding the current status of using RUOs in the clinical lab. He did add the disclaimer that these were his views and did not represent official FDA policy; and you thought it was confusing before!!!!! Don't shoot the messenger......... "-----Original Message----- From: Gutman, Steve [mailto:steve.gutman@fda.hhs.gov] Sent: Monday, January 29, 2007 2:52 PM To: Houston, Ronald Subject: RE: RUO's and ASR reagents in diagnostics - FDA disclaimer I have had informal discussions with CAP but FDA has not taken a formal position on use of RUOs to support home brews. In general, we think this is a dreadful choice and clearly a tactic of last resort. There are no regulatory requirements for RUO devices. So the laboratory takes full responsibility for ensuring quality of production of the RUO material being made over time. This seems to me to put laboratories at considerable liability risk since the general controls applied to ASRs don't work here. As we try to review our regulatory policy in this area, it is certainly one we should and will try to clarify. Thanks. Steve ________________________________ From: Houston, Ronald [mailto:HoustonR@chi.osu.edu] Sent: Monday, January 29, 2007 10:23 AM To: Gutman, Steve Subject: RUO's and ASR reagents in diagnostics - FDA disclaimer Importance: High Dr Gutman, There is tremendous confusion in laboratory circles regarding the FDA disclaimer for ASR antibodies and whether or not this same disclaimer can be used for RUO antibodies, particularly in light of CAP's change in interpretation of the guidelines for IHC testing: "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew test only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director. The laboratory must establish or verify the performance characteristics of tests using Class I ASR's and RUO's in accordance with the Method Performance Specifications section of the Laboratory General checklist." Exactly what is the current stance of the FDA, and have any discussions taken place between the FDA and CAP regarding their interpretation? Can results of RUO testing be dictated into a diagnostic report and can the test be billed? Obviously, antibody manufacturers mandate that their RUO antibodies cannot be used for diagnostic purposes, but they are as confused as the laboratorians. Thank you for taking the time to clarify this very confusing situation. Sincerely Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu " Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 26 Date: Tue, 30 Jan 2007 07:02:53 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Processing debate To: GMartin@marshallhospital.org, histonet@lists.utsouthwestern.edu Message-ID: <189401.17374.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Fear the word "random", in histology is almost synonym with "caos".There is absolutely no logic or benefit from placing the cassettes at random. If you use a cassetting log the sequencial and orderly manner of placing the cassettes following the log order is the way of doing things. You can always know where your cassettes are and at the embedding step you can follow the same sequency and easily determine if all the cassettes were processed and embeded. It is absolutely illogical to place anything at random (unless you are selecting something for an experimental design that requires that type of selection!). The only "logical" explanation to your initial question is that it stems from the pathologist's lazyness that prefers to throw the cassettes in the container and letting the histotech to try to organize what should not have been disorganized in the first place. Ren? J. GMartin@marshallhospital.org wrote: We presently process with a Tissue Tek Vip 2000. Our pathologist cut the tissue in. the debate is this ... one side says that the cassettes must be placed in a container of formalin to float at random before being placed into the Vip 2000 basket. The other side of the debate says that the tissue cassettes can be placed in order directly into the basket that is submerged in formalin. Can the group shine any light on this so far civil debate :) Thanks Gary Martin El Dorado Pathology California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 38, Issue 46 **************************************** Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From springrosycloud <@t> 126.com Wed Jan 31 06:40:01 2007 From: springrosycloud <@t> 126.com (=?gb2312?B?1qO0us+8?=) Date: Wed Jan 31 06:40:35 2007 Subject: [Histonet] Michel's transport medium Message-ID: <45C08E21.000005.18600@bj126app12.126.com> Hello, everyone. I am going to use Michel's transport medium to send renal tissue samples. I got a protocol on the medium preparations(see belows). When I made the preparation according to the protocol, I met troubles. At the first step(1.0 M potassium citrate buffer pH 7.0), I could not adjust pH using the 1M potassium hydroxide. I had to adjust pH to 7.0 using 10M potassium hydroxide(40ml). And then, I could not dissolve 55 grams of ammonium sulfate in 100 ml washing solution. About half of ammonium sulfate(made in China) kept unsolved, even though I incubated it in heat water and/or mechanical stirring. When I bought a bottle of new ammonium sulfate, it still did not work. Later, I tried to dissolve 55g ammonium sulfate in 100ml distilled water, but I failed. I tried and thought it again and again. I did not know what is the matter. Please tell me what I should do. I am waiting for your kindly reply. Sincerely, Betty Nanjing University School of Medicine, Research institute of Nephrology, Jinling Hospital, Nanjing, China 210002 Tel: 86-025-81765894 Fax: 86-025-84801992The protocol from http://publish.uwo.ca/~jkiernan/faqlist.htm#MICHELammonium sulfate was unsolved. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g citric acid monohydrate (or 19.2 g citric acid anhydrous) in 30 mL of hot deionized or distilled water. Cool. Adjust pH to 7.0 with 1 M potassium hydroxide (about 35 mL) Dilute to 100 mL with more water. Washing solution: 25 mL 1.0 M potassium citrate buffer 50 mL 0.1 M magnesium sulfate heptahydrate (F.W. 246.5) 50 mL 0.1 M N-ethyl maleimide (= 12.5 g in 1 L of water) (Sigma E3876.) Water to make 1 L Adjust to pH 7.0 with 1 M potassium hydroxide Store in refrigerator. (Cost about $50/25 g in 1994.) Transport medium: Dissolve 55 grams of ammonium sulfate in 100 mL washing solution. (Add slowly, with mechanical stirring.) Adjust pH to about 6.9 with 1 M potassium hydroxide (< 2 mL needed)Reference: Michel B. Milner Y. David K. Preservation of tissue-fixed immunoglobulins in skin biopsies of patients with lupus erythematosus and bullous diseases. A preliminary report. J. Invest. Dermatol. 59: 449-452 (1973). From TMcNemar <@t> lmhealth.org Wed Jan 31 08:29:56 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jan 31 08:30:03 2007 Subject: [Histonet] Michel's transport medium Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F452@lmhsmail.lmhealth.org> I would think that whoever you send them to should supply the necessary = transport medias. At least that's the case with everything I send out. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of = springrosycloud@126.com Sent: Wednesday, January 31, 2007 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Michel's transport medium Hello, everyone. I am going to use Michel's transport medium to send = renal tissue samples. I got a protocol on the medium preparations(see = belows). When I made the preparation according to the protocol, I met = troubles. At the first step(1.0 M potassium citrate buffer pH 7.0), I = could not adjust pH using the 1M potassium hydroxide. I had to adjust pH = to 7.0 using 10M potassium hydroxide(40ml). And then, I could not = dissolve 55 grams of ammonium sulfate in 100 ml washing solution. About = half of ammonium sulfate(made in China) kept unsolved, even though I = incubated it in heat water and/or mechanical stirring. When I bought a = bottle of new ammonium sulfate, it still did not work. Later, I tried to = dissolve 55g ammonium sulfate in 100ml distilled water, but I failed. I = tried and thought it again and again. I did not know what is the matter. = Please tell me what I should do. I am waiting for your kindly reply. = Sincerely, =20 Betty =20 Nanjing University School of Medicine,=20 Research institute of Nephrology, Jinling Hospital,=20 Nanjing, China 210002 Tel: 86-025-81765894 Fax: 86-025-84801992The protocol from = http://publish.uwo.ca/~jkiernan/faqlist.htm#MICHELammonium sulfate was = unsolved. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g = citric acid monohydrate (or 19.2 g citric acid anhydrous) in = 30 mL of hot deionized or distilled water. Cool. Adjust pH to 7.0 = with 1 M potassium hydroxide (about 35 mL) Dilute to 100 mL with more = water. Washing solution: 25 mL 1.0 M potassium citrate buffer = 50 mL 0.1 M magnesium sulfate heptahydrate (F.W. 246.5) 50 mL 0.1 = M N-ethyl maleimide (=3D 12.5 g in 1 L of water) (Sigma = E3876.) Water to make 1 L Adjust to pH 7.0 with 1 M potassium = hydroxide Store in refrigerator. (Cost about $50/25 g in 1994.) = Transport medium: Dissolve 55 grams of ammonium sulfate in 100 mL = washing solution. (Add slowly, with mechanical stirring.) = Adjust pH to about 6.9 with 1 M potassium hydroxide (< 2 mL = needed)Reference: Michel B. Milner Y.=20 David K. Preservation of tissue-fixed immunoglobulins in skin biopsies = of patients with lupus erythematosus and bullous diseases. A preliminary = report. J. Invest. Dermatol. 59: 449-452 (1973). =20 =20 =20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 31 08:42:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 31 08:42:45 2007 Subject: [Histonet] Michel's transport medium In-Reply-To: <45C08E21.000005.18600@bj126app12.126.com> Message-ID: <454080.21984.qm@web61216.mail.yahoo.com> I used to prepare Michel's solution in the following way: sol. 1- 1 M sol. of potassium citrate monohydrate----- 32.42 g/100mL dist. water sol. 2- 0.1 M sol. of magnesium sulfate hepta hydrated -------2.46 g/100 mL sol. 3- 0.1 M sol. of N-ethyl maleimide ---1.25 g/100 mL To prepare 1 litre of Michel's add: a- 25 mL of solution 1 b- 50 mL of solution 2 c- 50 mL of solution 3 d- 875 mL of distilled water, and e- 550 g of ammonium sulfate f- when all components are dissolved adjust pH to 7.0 using a 1M solution of potassium hydroxide. The solution should be kept between 0 and 6?C This procedure always worked fine with me. Try to use analysis grade chemicals. Hope this will help you. Ren? J. ?????? wrote: Hello, everyone. I am going to use Michel's transport medium to send renal tissue samples. I got a protocol on the medium preparations(see belows). When I made the preparation according to the protocol, I met troubles. At the first step(1.0 M potassium citrate buffer pH 7.0), I could not adjust pH using the 1M potassium hydroxide. I had to adjust pH to 7.0 using 10M potassium hydroxide(40ml). And then, I could not dissolve 55 grams of ammonium sulfate in 100 ml washing solution. About half of ammonium sulfate(made in China) kept unsolved, even though I incubated it in heat water and/or mechanical stirring. When I bought a bottle of new ammonium sulfate, it still did not work. Later, I tried to dissolve 55g ammonium sulfate in 100ml distilled water, but I failed. I tried and thought it again and again. I did not know what is the matter. Please tell me what I should do. I am waiting for your kindly reply. Sincerely, Betty Nanjing University School of Medicine, Research institute of Nephrology, Jinling Hospital, Nanjing, China 210002 Tel: 86-025-81765894 Fax: 86-025-84801992The protocol from http://publish.uwo.ca/~jkiernan/faqlist.htm#MICHELammonium sulfate was unsolved. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g citric acid monohydrate (or 19.2 g citric acid anhydrous) in 30 mL of hot deionized or distilled water. Cool. Adjust pH to 7.0 with 1 M potassium hydroxide (about 35 mL) Dilute to 100 mL with more water. Washing solution: 25 mL 1.0 M potassium citrate buffer 50 mL 0.1 M magnesium sulfate heptahydrate (F.W. 246.5) 50 mL 0.1 M N-ethyl maleimide (= 12.5 g in 1 L of water) (Sigma E3876.) Water to make 1 L Adjust to pH 7.0 with 1 M potassium hydroxide Store in refrigerator. (Cost about $50/25 g in 1994.) Transport medium: Dissolve 55 grams of ammonium sulfate in 100 mL washing solution. (Add slowly, with mechanical stirring.) Adjust pH to about 6.9 with 1 M potassium hydroxide (< 2 mL needed)Reference: Michel B. Milner Y. David K. Preservation of tissue-fixed immunoglobulins in skin biopsies of patients with lupus erythematosus and bullous diseases. A preliminary report. J. Invest. Dermatol. 59: 449-452 (1973). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never Miss an Email Stay connected with Yahoo! Mail on your mobile. Get started! From cmiller <@t> physlab.com Wed Jan 31 09:37:36 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jan 31 09:37:46 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: References: <831462.68750.qm@web51812.mail.yahoo.com><358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> Message-ID: <000801c7454d$bbc50c80$db01a8c0@plab.local> I cant believe they stopped the practicum part of the test. I just took it a few years back. I would never be the tech I am had I not had to practice and cut hundreds of slides looking for that perfect one or that perfect stain, tissue etc. Just my opinion, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, January 30, 2007 2:22 PM To: Ra; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM Are we discussing the HT or the HTL? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra Sent: Tue 1/30/2007 3:09 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] ASCP EXAM Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Wed Jan 31 09:40:05 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Wed Jan 31 09:40:14 2007 Subject: [Histonet] cytospins Message-ID: <45C0B855.7080809@tufts.edu> Hi all, I'm doing immunofluorescence on cytospins - and have had several problems. First, we're looking at a rare cell from a FACS sort - about 1000-1500 cells per ml. We're getting a big loss when we cytocentrifuge - about 60% loss. Does anyone have a recommendation for a cytocentrifuge that will conserve cells better than this? Second, how important is it to fix cells immediately? Our FACS sorts are done in Boston, then we travel about an hour to our lab. It is really tough to fix cells in Boston - our procedure is Cytofix at 4C for 20 minutes, then acetone at 4C for 10 minutes, followed by PBS wash, block, and overnight primaries. Will we experience serious cell deterioration if we don't fix them for an hour to two hours after sorting? I really appreciate any input. Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Tufts Cummings School of Veterinary Medicine 200 Westboro Road North Grafton, MA 01536 Tel:508-839-5395 Email: melissa.mazan@tufts.edu Fax:508-839-7922 From ryakay <@t> shands.ufl.edu Wed Jan 31 09:49:42 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Wed Jan 31 09:50:10 2007 Subject: [Histonet] ASCP EXAM Message-ID: Actually I totally agree with you. I have seen many people that are great at memorizing information and great at test taking but cannot for the life of them cut a quality section. In our HT program we will NOT get rid of the practicum project they must do while in their clinical rotations. If they don't pass their project, they do not pass the course. I think this change has really put more pressure on the schools to make sure we graduate students that know how to cut a decent section as well as competent in the areas of troubleshooting. We all hope that the schools do attain this goal but being in the profession of so many years I know that true troubleshooting abilities come with experience. Just my thoughts, Kaye Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> "Cheri Miller" 1/31/2007 10:37 AM >>> I cant believe they stopped the practicum part of the test. I just took it a few years back. I would never be the tech I am had I not had to practice and cut hundreds of slides looking for that perfect one or that perfect stain, tissue etc. Just my opinion, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, January 30, 2007 2:22 PM To: Ra; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM Are we discussing the HT or the HTL? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra Sent: Tue 1/30/2007 3:09 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] ASCP EXAM Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jan 31 09:45:11 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jan 31 09:54:18 2007 Subject: [Histonet] ASCP EXAM References: <831462.68750.qm@web51812.mail.yahoo.com><358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> <000801c7454d$bbc50c80$db01a8c0@plab.local> Message-ID: I agree. I know of one individual with a Master's that will be sitting for her HTL soon and has never done a single special in her life, except for grams in micro. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, January 31, 2007 10:38 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); 'Ra'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM I cant believe they stopped the practicum part of the test. I just took it a few years back. I would never be the tech I am had I not had to practice and cut hundreds of slides looking for that perfect one or that perfect stain, tissue etc. Just my opinion, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, January 30, 2007 2:22 PM To: Ra; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM Are we discussing the HT or the HTL? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra Sent: Tue 1/30/2007 3:09 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] ASCP EXAM Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> charter.net Wed Jan 31 09:59:10 2007 From: pathrm35 <@t> charter.net (pathrm35@charter.net) Date: Wed Jan 31 09:59:18 2007 Subject: [Histonet] ASCP EXAM Message-ID: <1891315748.1170259150324.JavaMail.root@fepweb09> I agree with you Kaye. This is the way future techs should be trained. I have some lab assistants where I currently work who want me to OJT them. I refuse to because they are not in school or in a histo program. Just my two cents. Ron Martin, BS, HT (ASCP) HTL, QIHC ---- Kaye Ryan wrote: > Actually I totally agree with you. I have seen many people that are > great at memorizing information and great at test taking but cannot for > the life of them cut a quality section. In our HT program we will NOT > get rid of the practicum project they must do while in their clinical > rotations. If they don't pass their project, they do not pass the > course. I think this change has really put more pressure on the schools > to make sure we graduate students that know how to cut a decent section > as well as competent in the areas of troubleshooting. We all hope that > the schools do attain this goal but being in the profession of so many > years I know that true troubleshooting abilities come with experience. > > Just my thoughts, > Kaye > > Kaye Ryan > Histology Manager/Educational Coordinator > Shands Rocky Point Laboratories > (352) 265-0111, 72093 > > >>> "Cheri Miller" 1/31/2007 10:37 AM >>> > I cant believe they stopped the practicum part of the test. I just took > it > a few years back. I would never be the tech I am had I not had to > practice > and cut hundreds of slides looking for that perfect one or that > perfect > stain, tissue etc. Just my opinion, Cheri > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, > Jeanine (CDC/CCID/NCZVED) > Sent: Tuesday, January 30, 2007 2:22 PM > To: Ra; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP EXAM > > Are we discussing the HT or the HTL? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra > > Sent: Tue 1/30/2007 3:09 PM > To: Histonet@lists.utsouthwestern.edu > Cc: > Subject: Re: [Histonet] ASCP EXAM > > > > Funny how that test is so different every time... I found the > opposite to > be true. I had some questions on my cert. exam that were word > for > word out > of the ASCP book. I found the NSH series to be more of a > waste. > > Good luck to anyone taking the test! > Rhonda B. > > On 1/29/07, MICHELLE SEAGLE wrote: > > > > Don't waste your time or money on the ASCP practical exam > questions, what > > a waste. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Jan 31 10:00:28 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jan 31 10:00:38 2007 Subject: [Histonet] cytospins Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012471CE@wahtntex2.waht.swest.nhs.uk> Hi all, I'm doing immunofluorescence on cytospins - and have had several problems. First, we're looking at a rare cell from a FACS sort - about 1000-1500 cells per ml. We're getting a big loss when we cytocentrifuge - about 60% loss. Does anyone have a recommendation for a cytocentrifuge that will conserve cells better than this? Second, how important is it to fix cells immediately? Our FACS sorts are done in Boston, then we travel about an hour to our lab. It is really tough to fix cells in Boston - our procedure is Cytofix at 4C for 20 minutes, then acetone at 4C for 10 minutes, followed by PBS wash, block, and overnight primaries. Will we experience serious cell deterioration if we don't fix them for an hour to two hours after sorting? I really appreciate any input. Melissa I had similar problems when doing my Masters; cell loss from Cytospins in poorly cellular specimens is a real problem. I was never sure if the cells went into the filter paper or if they fell off. Urine preparation has similar problems; they are poorly cellular and very watery. The usual method is to add bovine albumin so when you spray fix the protein glues the cells to the glass but it was still never satisfactory. We tried Nuclepore preparations and they were better but you had the filter to look through. Liquid based cytology IMHO is the only way to do this; it solves the fixation problem as they should be fixed ASAP and harvests many more cells. If you can't do that then oddly you might get better results by spinning the whole urine sample down and doing direct smears fixed ASAP. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com There are many things in life that will catch your eye, but only a few will catch your heart...pursue those. --Michael Nolan This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ander093 <@t> tc.umn.edu Wed Jan 31 10:23:45 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Jan 31 10:24:06 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: <000801c7454d$bbc50c80$db01a8c0@plab.local> References: <831462.68750.qm@web51812.mail.yahoo.com> <358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> <000801c7454d$bbc50c80$db01a8c0@plab.local> Message-ID: <6.2.3.4.0.20070131102020.03b0ae80@ander093.email.umn.edu> I couldn't agree more. I think that the practical is the more important part of the exam. The stuff on the written can always be looked up--but just knowing what is in a fixative etc. does not mean one can cut good sections or troubleshoot a stain. My boss--a busy Neuropathologist does not relish the idea of having to include cutting, staining as part of a hiring interview!!! LuAnn Anderson HT(ASCP) At 09:37 AM 1/31/2007, Cheri Miller wrote: >I cant believe they stopped the practicum part of the test. I just took it >a few years back. I would never be the tech I am had I not had to practice >and cut hundreds of slides looking for that perfect one or that perfect >stain, tissue etc. Just my opinion, Cheri > >Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >Jeanine (CDC/CCID/NCZVED) >Sent: Tuesday, January 30, 2007 2:22 PM >To: Ra; Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] ASCP EXAM > >Are we discussing the HT or the HTL? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra > Sent: Tue 1/30/2007 3:09 PM > To: Histonet@lists.utsouthwestern.edu > Cc: > Subject: Re: [Histonet] ASCP EXAM > > > > Funny how that test is so different every time... I found the >opposite to > be true. I had some questions on my cert. exam that were word for >word out > of the ASCP book. I found the NSH series to be more of a waste. > > Good luck to anyone taking the test! > Rhonda B. > > On 1/29/07, MICHELLE SEAGLE wrote: > > > > Don't waste your time or money on the ASCP practical exam >questions, what > > a waste. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emilie.belanger.trudelle <@t> UMontreal.CA Wed Jan 31 10:25:51 2007 From: emilie.belanger.trudelle <@t> UMontreal.CA (=?iso-8859-1?b?yW1pbGllIA==?= =?iso-8859-1?b?QulsYW5nZXI=?= Trudelle) Date: Wed Jan 31 10:26:01 2007 Subject: [Histonet] F4/80 help Message-ID: <1170260751.45c0c30f9d21b@www.courrier.umontreal.ca> Hi, I'm using F4/80 (Serotec) for IHC on frozen mouse spleen. I'm using biotinylated anti-rat IgG from Vector and Vectastain ABC kit and DAB also from Vector . I have try a lot of different protocols and incubation time with F4/80 and i always obtain yellow granules around my positive cells and on my isotypic negative control as well. Does any one know what these granules are and how to block their coloration???? Thanks Emilie B?langer Universit? de Montr?al From leswes <@t> shaw.ca Wed Jan 31 10:36:05 2007 From: leswes <@t> shaw.ca (Lesley Weston) Date: Wed Jan 31 10:38:50 2007 Subject: [Histonet] bone softening In-Reply-To: <9B4A77DF11463E4FB723D484214AE9BC02517535@KALEXMB02.KaleidaHealth.org> Message-ID: If you have time, you could try clearing in cedarwood oil instead of xylene, It leaves the tissue much softer and less brittle, but it does take days, even weeks, and it's quite expensive. You might get some improvement by using chloroform to clear instead. Another possibility would be to soak the bone in fabric softener (any brand) for a day or two after decalcifying it and before dehydrating it; I've never done this, but I've heard it works. -- Lesley Weston > From: "DiCarlo, Margaret" > Date: Tue, 30 Jan 2007 10:58:00 -0500 > To: histonet@pathology.swmed.edu > Subject: [Histonet] bone softening > > Histonetters, > > > > A while ago I remember someone mentioning a method to soften bone after > it's been decaled but prior to processing. Can anyone tell me the > procedure and does it have any affect on the H&E staining? Lately, my 5 > x 7" size bones have been giving me great difficulty when sectioning > since they have been very dense and hard. I process with xylene. > > > > Thanks for your help. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedics Bone Lab > > Buffalo General Hospital > > 100 High St. > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. > > Check us out at: www.WesternNYshospitalofchoice.org > > CONFIDENTIALITY NOTICE: > This email transmission and any documents, files, > or previous e-mail messages attached to it are > confidential and intended solely for the use of the > individual or entity to whom they are addressed. > If you are not the intended recipient, or a person > responsible for delivering it to the intended recipient, > you are hereby notified that any further review, > disclosure, copying, dissemination, distribution, or > use of any of the information contained in or attached > to this e-mail transmission is strictly prohibited. > If you have received this message in error, please > notify the sender immediately by e-mail, discard > any paper copies, and delete all electronic files > of the message. If you are unable to contact the > sender or you are not sure as to whether you > are the intended recipient, please e-mail > ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jao7 <@t> columbia.edu Wed Jan 31 11:00:03 2007 From: jao7 <@t> columbia.edu (Juan Oliver M.D.) Date: Wed Jan 31 11:00:11 2007 Subject: [Histonet] DAPI Message-ID: <45C0CB13.4080409@columbia.edu> For immuno fluorescence, does anybody know what (blue) dye effectivelly stains nuclei (DNA) when sections have been treated with 2 N HCl for BrdU detection? Thanks. oliveralonso From JMacDonald <@t> mtsac.edu Wed Jan 31 11:03:15 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 31 11:03:23 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: <1891315748.1170259150324.JavaMail.root@fepweb09> Message-ID: The students in our program must hand in practical slides for grading throughout the semester, for 3 of our techniques classes. They also must complete 240 hours of work experience in a histology laboratory. One of the problems that I saw with the ASCP practical was the amount of cheating that went on. Most are honest and do their best to hand in their own best work, but I know of facilities that were staining the slides and handing them to the applicants. This is not a true measure of what each applicant is capable of. There were many applicants that handed in slides and had no idea what they were. The collection of the tissue is often done by someone else. Automation is used to stain and coverslip and someone other than the applicant reviews the end product. The "new" exam format should test for knowledge and the ability to trouble shoot. The only two routes for the exam are graduation from a NAACLS accredited program or an AS degree (or equivalent) and one year experience. It is expected that in the practical part of a HT program or during the 1 year experience the applicant develops the necessary microtomy skills. Jennifer MacDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 07:59 AM To Cheri Miller , 'Ra' , Histonet@lists.utsouthwestern.edu, "Jeanine (CDC/CCID/NCZVED)' 'Bartlett" , Kaye Ryan cc Subject RE: [Histonet] ASCP EXAM I agree with you Kaye. This is the way future techs should be trained. I have some lab assistants where I currently work who want me to OJT them. I refuse to because they are not in school or in a histo program. Just my two cents. Ron Martin, BS, HT (ASCP) HTL, QIHC ---- Kaye Ryan wrote: > Actually I totally agree with you. I have seen many people that are > great at memorizing information and great at test taking but cannot for > the life of them cut a quality section. In our HT program we will NOT > get rid of the practicum project they must do while in their clinical > rotations. If they don't pass their project, they do not pass the > course. I think this change has really put more pressure on the schools > to make sure we graduate students that know how to cut a decent section > as well as competent in the areas of troubleshooting. We all hope that > the schools do attain this goal but being in the profession of so many > years I know that true troubleshooting abilities come with experience. > > Just my thoughts, > Kaye > > Kaye Ryan > Histology Manager/Educational Coordinator > Shands Rocky Point Laboratories > (352) 265-0111, 72093 > > >>> "Cheri Miller" 1/31/2007 10:37 AM >>> > I cant believe they stopped the practicum part of the test. I just took > it > a few years back. I would never be the tech I am had I not had to > practice > and cut hundreds of slides looking for that perfect one or that > perfect > stain, tissue etc. Just my opinion, Cheri > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, > Jeanine (CDC/CCID/NCZVED) > Sent: Tuesday, January 30, 2007 2:22 PM > To: Ra; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP EXAM > > Are we discussing the HT or the HTL? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra > > Sent: Tue 1/30/2007 3:09 PM > To: Histonet@lists.utsouthwestern.edu > Cc: > Subject: Re: [Histonet] ASCP EXAM > > > > Funny how that test is so different every time... I found the > opposite to > be true. I had some questions on my cert. exam that were word > for > word out > of the ASCP book. I found the NSH series to be more of a > waste. > > Good luck to anyone taking the test! > Rhonda B. > > On 1/29/07, MICHELLE SEAGLE wrote: > > > > Don't waste your time or money on the ASCP practical exam > questions, what > > a waste. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Wed Jan 31 11:00:36 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Wed Jan 31 11:10:10 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: <000801c7454d$bbc50c80$db01a8c0@plab.local> References: <831462.68750.qm@web51812.mail.yahoo.com> <358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> <000801c7454d$bbc50c80$db01a8c0@plab.local> Message-ID: Don't you still have to have at least 2 years experience in the lab before you sit for the exam? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Cheri Miller Date: Wednesday, January 31, 2007 9:48 am Subject: RE: [Histonet] ASCP EXAM To: "'Bartlett, Jeanine (CDC/CCID/NCZVED)'" , 'Ra' , Histonet@lists.utsouthwestern.edu > I cant believe they stopped the practicum part of the test. I just > took it > a few years back. I would never be the tech I am had I not had to > practiceand cut hundreds of slides looking for that perfect one or > that perfect > stain, tissue etc. Just my opinion, Cheri > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett,Jeanine (CDC/CCID/NCZVED) > Sent: Tuesday, January 30, 2007 2:22 PM > To: Ra; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP EXAM > > Are we discussing the HT or the HTL? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra > Sent: Tue 1/30/2007 3:09 PM > To: Histonet@lists.utsouthwestern.edu > Cc: > Subject: Re: [Histonet] ASCP EXAM > > > > Funny how that test is so different every time... I found the > opposite to > be true. I had some questions on my cert. exam that were word for > word out > of the ASCP book. I found the NSH series to be more of a waste. > > Good luck to anyone taking the test! > Rhonda B. > > On 1/29/07, MICHELLE SEAGLE wrote: > > > > Don't waste your time or money on the ASCP practical exam > questions, what > > a waste. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Wed Jan 31 11:17:23 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 31 11:17:29 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: Message-ID: One year is required with an AS degree. The two year requirement was for Route 3, which was discontinued at the end of 2005. Jennifer MacDonald histology@gradymem.org Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 09:00 AM To Cheri Miller cc Histonet@lists.utsouthwestern.edu Subject Re: RE: [Histonet] ASCP EXAM Don't you still have to have at least 2 years experience in the lab before you sit for the exam? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Cheri Miller Date: Wednesday, January 31, 2007 9:48 am Subject: RE: [Histonet] ASCP EXAM To: "'Bartlett, Jeanine (CDC/CCID/NCZVED)'" , 'Ra' , Histonet@lists.utsouthwestern.edu > I cant believe they stopped the practicum part of the test. I just > took it > a few years back. I would never be the tech I am had I not had to > practiceand cut hundreds of slides looking for that perfect one or > that perfect > stain, tissue etc. Just my opinion, Cheri > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett,Jeanine (CDC/CCID/NCZVED) > Sent: Tuesday, January 30, 2007 2:22 PM > To: Ra; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP EXAM > > Are we discussing the HT or the HTL? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra > Sent: Tue 1/30/2007 3:09 PM > To: Histonet@lists.utsouthwestern.edu > Cc: > Subject: Re: [Histonet] ASCP EXAM > > > > Funny how that test is so different every time... I found the > opposite to > be true. I had some questions on my cert. exam that were word for > word out > of the ASCP book. I found the NSH series to be more of a waste. > > Good luck to anyone taking the test! > Rhonda B. > > On 1/29/07, MICHELLE SEAGLE wrote: > > > > Don't waste your time or money on the ASCP practical exam > questions, what > > a waste. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Jan 31 11:23:22 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jan 31 11:23:36 2007 Subject: [Histonet] F4/80 help Message-ID: <813244.20750.qm@web50302.mail.yahoo.com> Those yellow/brown granules are probably hemosiderin, a type of iron pigment that is abundant in mouse spleens. I don't think you can do anything to get rid of them, but maybe someone else on this list knows. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: ?milie B?langer Trudelle To: histonet@lists.utsouthwestern.edu Sent: Wednesday, January 31, 2007 11:25:51 AM Subject: [Histonet] F4/80 help Hi, I'm using F4/80 (Serotec) for IHC on frozen mouse spleen. I'm using biotinylated anti-rat IgG from Vector and Vectastain ABC kit and DAB also from Vector . I have try a lot of different protocols and incubation time with F4/80 and i always obtain yellow granules around my positive cells and on my isotypic negative control as well. Does any one know what these granules are and how to block their coloration???? Thanks Emilie B?langer Universit? de Montr?al _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news From caron_fournier <@t> yahoo.ca Wed Jan 31 11:31:18 2007 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Wed Jan 31 11:31:29 2007 Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 49 Message-ID: <226521.77195.qm@web35403.mail.mud.yahoo.com> I was wondering if anyone has a method for dissolving PMMA and not harming the bone that is embedded in it? Caron Fournier, BSc, RT caron_fournier@yahoo.ca __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ploykasek <@t> phenopath.com Wed Jan 31 11:37:33 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jan 31 11:37:59 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: <6.2.3.4.0.20070131102020.03b0ae80@ander093.email.umn.edu> Message-ID: I was disappointed that the practical was discontinued, but I know there were many logistical and other reasons for this. It has been our practice for several years to administer a microtomy test to all registered histology job applicants. At first, I was uncomfortable asking applicants to do this but thought it was a 'necessary evil'. I have found all skill levels of cutting, and that this does not necessarily relate to level of certification, years of experience or resume. I have not asked anyone to perform any stains, but I do ask questions relating to basic procedures. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I couldn't agree more. I think that the practical is the more > important part of the exam. The stuff on the written can always be > looked up--but just knowing what is in a fixative etc. does not mean > one can cut good sections or troubleshoot a stain. My boss--a busy > Neuropathologist does not relish the idea of having to include > cutting, staining as part of a hiring interview!!! > > LuAnn Anderson HT(ASCP) > > > At 09:37 AM 1/31/2007, Cheri Miller wrote: >> I cant believe they stopped the practicum part of the test. I just took it >> a few years back. I would never be the tech I am had I not had to practice >> and cut hundreds of slides looking for that perfect one or that perfect >> stain, tissue etc. Just my opinion, Cheri >> >> Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Tuesday, January 30, 2007 2:22 PM >> To: Ra; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] ASCP EXAM >> >> Are we discussing the HT or the HTL? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra >> Sent: Tue 1/30/2007 3:09 PM >> To: Histonet@lists.utsouthwestern.edu >> Cc: >> Subject: Re: [Histonet] ASCP EXAM >> >> >> >> Funny how that test is so different every time... I found the >> opposite to >> be true. I had some questions on my cert. exam that were word for >> word out >> of the ASCP book. I found the NSH series to be more of a waste. >> >> Good luck to anyone taking the test! >> Rhonda B. >> >> On 1/29/07, MICHELLE SEAGLE wrote: >>> >>> Don't waste your time or money on the ASCP practical exam >> questions, what >>> a waste. >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From GMartin <@t> marshallhospital.org Wed Jan 31 11:42:05 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Wed Jan 31 11:40:08 2007 Subject: [Histonet] Histo tech requirements Message-ID: What are the requirements for a certified histo tech ... and are there schools in California? From ryaskovich <@t> dir.nidcr.nih.gov Wed Jan 31 11:45:07 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Wed Jan 31 11:45:22 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: References: <6.2.3.4.0.20070131102020.03b0ae80@ander093.email.umn.edu> Message-ID: Why would you get an associates degree, take an exam, then apply for a job you can't do? Just a question. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neurobiology and Pain Therapeutics Branch -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, January 31, 2007 12:38 PM To: histonet Subject: Re: [Histonet] ASCP EXAM I was disappointed that the practical was discontinued, but I know there were many logistical and other reasons for this. It has been our practice for several years to administer a microtomy test to all registered histology job applicants. At first, I was uncomfortable asking applicants to do this but thought it was a 'necessary evil'. I have found all skill levels of cutting, and that this does not necessarily relate to level of certification, years of experience or resume. I have not asked anyone to perform any stains, but I do ask questions relating to basic procedures. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I couldn't agree more. I think that the practical is the more > important part of the exam. The stuff on the written can always be > looked up--but just knowing what is in a fixative etc. does not mean > one can cut good sections or troubleshoot a stain. My boss--a busy > Neuropathologist does not relish the idea of having to include > cutting, staining as part of a hiring interview!!! > > LuAnn Anderson HT(ASCP) > > > At 09:37 AM 1/31/2007, Cheri Miller wrote: >> I cant believe they stopped the practicum part of the test. I just took it >> a few years back. I would never be the tech I am had I not had to practice >> and cut hundreds of slides looking for that perfect one or that perfect >> stain, tissue etc. Just my opinion, Cheri >> >> Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Tuesday, January 30, 2007 2:22 PM >> To: Ra; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] ASCP EXAM >> >> Are we discussing the HT or the HTL? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra >> Sent: Tue 1/30/2007 3:09 PM >> To: Histonet@lists.utsouthwestern.edu >> Cc: >> Subject: Re: [Histonet] ASCP EXAM >> >> >> >> Funny how that test is so different every time... I found the >> opposite to >> be true. I had some questions on my cert. exam that were word for >> word out >> of the ASCP book. I found the NSH series to be more of a waste. >> >> Good luck to anyone taking the test! >> Rhonda B. >> >> On 1/29/07, MICHELLE SEAGLE wrote: >>> >>> Don't waste your time or money on the ASCP practical exam >> questions, what >>> a waste. >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> gmhsc.com Wed Jan 31 12:11:04 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Wed Jan 31 12:11:19 2007 Subject: [Histonet] stain to r/o cat scratch Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F06D@exchange.gmhpost.com> Histonetters, I need your help....... Does anyone know of a stain other than the Warthin-Starry to rule out cat scratch? Thanks in advance... Amy Self/HT(ASCP) Georgetown Memorial Hospital Georgetown, SC NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From tim.morken <@t> thermofisher.com Wed Jan 31 12:29:04 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Jan 31 12:29:07 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: Message-ID: Ruth asks; "Why would you get an associates degree, take an exam, then apply for a job you can't do? Just a question." Most of the resumes I get (dozens) are people with absolutely no experience in histology (most are in biochemistry). Even when I do find a real histotech, the pay compititon is brutal due to all the big biotech firms in the area soaking up all the techs. It is so hard to find experienced histology people that we mostly hire either college grads or people from other fields whom we train. I would say the most valuable skill a current histology manager could have is the ability to train people on the job! The tough part is determining if a person is capable of doing the job - especially sectioning. In order to get excellent sectioning skills we usually end up hiring hospital Histotechs on a part time basis to do only that. So, if a person is interested in histology and takes courses and even a certification test with that in mind, I think that would be a person to take a serious look at since at least they have the theoretical background to understand the job. Tim Morken ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Wednesday, January 31, 2007 9:45 AM To: Patti Loykasek; histonet Subject: RE: [Histonet] ASCP EXAM Why would you get an associates degree, take an exam, then apply for a job you can't do? Just a question. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neurobiology and Pain Therapeutics Branch -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, January 31, 2007 12:38 PM To: histonet Subject: Re: [Histonet] ASCP EXAM I was disappointed that the practical was discontinued, but I know there were many logistical and other reasons for this. It has been our practice for several years to administer a microtomy test to all registered histology job applicants. At first, I was uncomfortable asking applicants to do this but thought it was a 'necessary evil'. I have found all skill levels of cutting, and that this does not necessarily relate to level of certification, years of experience or resume. I have not asked anyone to perform any stains, but I do ask questions relating to basic procedures. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I couldn't agree more. I think that the practical is the more > important part of the exam. The stuff on the written can always be > looked up--but just knowing what is in a fixative etc. does not mean > one can cut good sections or troubleshoot a stain. My boss--a busy > Neuropathologist does not relish the idea of having to include > cutting, staining as part of a hiring interview!!! > > LuAnn Anderson HT(ASCP) > > > At 09:37 AM 1/31/2007, Cheri Miller wrote: >> I cant believe they stopped the practicum part of the test. I just took it >> a few years back. I would never be the tech I am had I not had to practice >> and cut hundreds of slides looking for that perfect one or that perfect >> stain, tissue etc. Just my opinion, Cheri >> >> Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Tuesday, January 30, 2007 2:22 PM >> To: Ra; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] ASCP EXAM >> >> Are we discussing the HT or the HTL? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra >> Sent: Tue 1/30/2007 3:09 PM >> To: Histonet@lists.utsouthwestern.edu >> Cc: >> Subject: Re: [Histonet] ASCP EXAM >> >> >> >> Funny how that test is so different every time... I found the >> opposite to >> be true. I had some questions on my cert. exam that were word for >> word out >> of the ASCP book. I found the NSH series to be more of a waste. >> >> Good luck to anyone taking the test! >> Rhonda B. >> >> On 1/29/07, MICHELLE SEAGLE wrote: >>> >>> Don't waste your time or money on the ASCP practical exam >> questions, what >>> a waste. >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jan 31 12:35:07 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 31 12:35:42 2007 Subject: [Histonet] stain to r/o cat scratch In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F06D@exchange.gmhpost.com> References: <39836CD6DB61654E8F95A35898C9218602E4F06D@exchange.gmhpost.com> Message-ID: <45C09B0B0200007700004012@hcnwgwds01.hh.chs> Immunohistochemistry, in my opinion, is the best test. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Amy Self" 01/31/07 1:11 PM >>> Histonetters, I need your help....... Does anyone know of a stain other than the Warthin-Starry to rule out cat scratch? Thanks in advance... Amy Self/HT(ASCP) Georgetown Memorial Hospital Georgetown, SC NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From DMSmith <@t> ghsnet.org Wed Jan 31 12:48:41 2007 From: DMSmith <@t> ghsnet.org (Donna Smith) Date: Wed Jan 31 12:45:39 2007 Subject: [Histonet] Specimen transport Message-ID: Is anyone transporting surgical and/or cytology specimens (fresh, for frozen, containing formalin) in a pneumatic tube system? If so, how is it working? What did you do to put it in place i.e. test runs, etc?? Thanks Donna M. Smith H.T. (ASCP) Pathology Manager Gwinnett Medical Center 678-442-4206 dmsmith@ghsnet.org This email communication, including any attached files, may contain material that is protected, proprietary,privileged, confidential, or otherwise legally exempt from disclosure. This communication is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient or the person responsible for delivering this communication to the intended recipient, you are prohibited from retaining, using, disseminating, forwarding, printing or copying this communication or taking any action based on the contents of this communication. If you have received this communication in error, please immediately notify the sender by replying to this email, and then delete the original message and attachments. From Jessica.Vacca <@t> HCAhealthcare.com Wed Jan 31 13:07:30 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Wed Jan 31 13:07:06 2007 Subject: [Histonet] Breast implants Message-ID: <41E16A15CE78374EA45B57E0F94339B8020582A8@ORLEV01.hca.corpad.net> I was wondering what other facilities do with their breast implants, I have a bunch that have been stored since 1995...........How have others gone about disposing of them.....send letters to patients, informing them that will be discarded in 30 days, continue saving them........ Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From DOOLEEO <@t> shands.ufl.edu Wed Jan 31 13:10:30 2007 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Wed Jan 31 13:11:54 2007 Subject: [Histonet] trypsin and Chymotrypsin Message-ID: Dear Histonetters, Does anyone know a vendor for anti-Trypsin and anti-Chymotrypsin antibodies that work on formalin fixed paraffin embedded tissue. I have alpha-1 antitrypsin and alpha 1 antichymotrypsin but our pathologists do not want the "alpha 1" type of these antibodies. Thanks in advance Elaine Dooley HTL 352-265-0111 ext 72117 From nfournier <@t> sasktel.net Wed Jan 31 13:28:43 2007 From: nfournier <@t> sasktel.net (N Fournier) Date: Wed Jan 31 13:28:53 2007 Subject: [Histonet] Fisher Superfrost Plus slides Message-ID: <731befd57986.45c0998b@sasktel.net> Hello, I have encountered a few issues with Superfrost Plus slides recently and was wondering if anyone had any suggestios to remedy the situation. I am mounting fixed 40 um thick rat brain sections on to these slides. The instructions indicate that you should use warm dH2O in the mounting bath. However, when we place our tissue into water solution the tissue begins to fold and curl. This increases the level of wrinkling of the section once it has been mounted on to the slide. I have used PB as the bath medium previously and we never encounter this problem; however, experience has taught us that some tissue curling occurs when the slides are run through standard alcohol/xylene steps before coverslipping. So does anyone have specific suggestions? Thanks, Neil From bakevictoria <@t> gmail.com Wed Jan 31 13:34:09 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Jan 31 13:34:17 2007 Subject: [Histonet] Paper biopsy bags also called "tea bags" Message-ID: <4f016b690701311134l7b6bd561nc354cacfea389ca2@mail.gmail.com> Hi Everyone, I'm looking to buy the paper biopsy bags for our smaller tissues. We used to get them from Fisher, but they have discontinued it. Is anyone out there getting these still? Thanks in advance. Vikki Baker From LChen <@t> mednet.ucla.edu Wed Jan 31 13:42:06 2007 From: LChen <@t> mednet.ucla.edu (Chen, Leslie) Date: Wed Jan 31 13:42:26 2007 Subject: [Histonet] ASCP Exam Message-ID: <9F8AE7E7B303F44DB0CAB587F1E96C3F0A58D00D@admedmail3.ad.medctr.ucla.edu> I'm sorry, I'm confused about the conversations regarding the practical portion of the exam. Are you saying that the practical part is no longer required for HT or HTL cert for ASCP? According to their website, the practical is still required. There are practical instructions: https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el igibility/htl.aspx Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From Jackie.O'Connor <@t> abbott.com Wed Jan 31 14:00:16 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jan 31 14:00:52 2007 Subject: [Histonet] Paper biopsy bags also called "tea bags" In-Reply-To: <4f016b690701311134l7b6bd561nc354cacfea389ca2@mail.gmail.com> Message-ID: Surgipath has them. "Victoria Baker" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 01:34 PM To "Histo Net list server" cc Subject [Histonet] Paper biopsy bags also called "tea bags" Hi Everyone, I'm looking to buy the paper biopsy bags for our smaller tissues. We used to get them from Fisher, but they have discontinued it. Is anyone out there getting these still? Thanks in advance. Vikki Baker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jan 31 14:01:45 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 31 14:01:53 2007 Subject: AW: [Histonet] Specimen transport In-Reply-To: Message-ID: <000001c74572$a35a4960$c812a8c0@dielangs.at> Yes, we do so for our frozens. The system is established in the hospital, with some "ports". I don't know the technical background. If you want to send something, you have to dial the right number of the destination, set the "bomb" in the system and "wush".... It works fine with all tissue, that is small enough to fit in the container. The tissue is in plastic bags and I have never seen tissuefluids leaking out. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Donna Smith Gesendet: Mittwoch, 31. J?nner 2007 19:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Specimen transport Is anyone transporting surgical and/or cytology specimens (fresh, for frozen, containing formalin) in a pneumatic tube system? If so, how is it working? What did you do to put it in place i.e. test runs, etc?? Thanks Donna M. Smith H.T. (ASCP) Pathology Manager Gwinnett Medical Center 678-442-4206 dmsmith@ghsnet.org This email communication, including any attached files, may contain material that is protected, proprietary,privileged, confidential, or otherwise legally exempt from disclosure. This communication is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient or the person responsible for delivering this communication to the intended recipient, you are prohibited from retaining, using, disseminating, forwarding, printing or copying this communication or taking any action based on the contents of this communication. If you have received this communication in error, please immediately notify the sender by replying to this email, and then delete the original message and attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DeBrosse_Beatrice <@t> Allergan.com Wed Jan 31 14:13:10 2007 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Wed Jan 31 14:13:47 2007 Subject: [Histonet] Paper biopsy bags also called "tea bags" Message-ID: You can get them at Fisher (1-800-766-7000) #22-26-9314, 1000 per box. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, January 31, 2007 12:00 PM To: Victoria Baker Cc: Histo Net list server; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Paper biopsy bags also called "tea bags" Surgipath has them. "Victoria Baker" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 01:34 PM To "Histo Net list server" cc Subject [Histonet] Paper biopsy bags also called "tea bags" Hi Everyone, I'm looking to buy the paper biopsy bags for our smaller tissues. We used to get them from Fisher, but they have discontinued it. Is anyone out there getting these still? Thanks in advance. Vikki Baker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Wed Jan 31 14:24:10 2007 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Wed Jan 31 14:26:29 2007 Subject: [Histonet] RE: DAKO Vendor Message-ID: <20070131.122427.14831.1809012@webmail38.nyc.untd.com> Histonetters, I need to contact a DAKO Rep for info on the HBME-1 antibody. Anyone have any information for me? Thank you. Marsha Price, HT(ASCP) From rjbuesa <@t> yahoo.com Wed Jan 31 14:40:44 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 31 14:40:53 2007 Subject: [Histonet] stain to r/o cat scratch In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F06D@exchange.gmhpost.com> Message-ID: <628551.1070.qm@web61219.mail.yahoo.com> Amy: I used modified Steiner (phosphotungstic acid instead of uranyl nitrate). Ren? J. Amy Self wrote: Histonetters, I need your help....... Does anyone know of a stain other than the Warthin-Starry to rule out cat scratch? Thanks in advance... Amy Self/HT(ASCP) Georgetown Memorial Hospital Georgetown, SC NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From settembr <@t> umdnj.edu Wed Jan 31 14:40:28 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jan 31 14:41:31 2007 Subject: [Histonet] RE: DAKO Vendor Message-ID: Dako can put you in touch with their rep in your area. 1-800-235-5743 Their technical dept. is also very helpful and can give you info on HBME-1 1-800-424-0021 Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "mprice26@juno.com" 01/31/07 3:24 PM >>> Histonetters, I need to contact a DAKO Rep for info on the HBME-1 antibody. Anyone have any information for me? Thank you. Marsha Price, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 31 14:43:02 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 31 14:43:12 2007 Subject: [Histonet] Breast implants In-Reply-To: <41E16A15CE78374EA45B57E0F94339B8020582A8@ORLEV01.hca.corpad.net> Message-ID: <135635.15762.qm@web61215.mail.yahoo.com> Seek and follow the advise from your legal department. Treatment of breast implants vary widely from one place to the other. Ren? J. Vacca Jessica wrote: I was wondering what other facilities do with their breast implants, I have a bunch that have been stored since 1995...........How have others gone about disposing of them.....send letters to patients, informing them that will be discarded in 30 days, continue saving them........ Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From ekempf <@t> bioanalytical.com Wed Jan 31 15:21:24 2007 From: ekempf <@t> bioanalytical.com (Emily D. Kempf) Date: Wed Jan 31 15:21:32 2007 Subject: [Histonet] Unsubscribe Message-ID: <3100BDBD96C0724D9CE9E476BFB57D9652D5CB@basi10003.BIOANALYTICAL.COM> Please unsubscribe me Emily Kempf, B.S., HT (ASCP) Histology Technician BASi Evansville 10424 Middle Mt. Vernon Road Mt. Vernon, IN 47620 812.985.5900 X148 Fax 812.985.3403 From JMacDonald <@t> mtsac.edu Wed Jan 31 15:32:25 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 31 15:32:31 2007 Subject: [Histonet] ASCP Exam In-Reply-To: <9F8AE7E7B303F44DB0CAB587F1E96C3F0A58D00D@admedmail3.ad.medctr.ucla.edu> Message-ID: The practical will only apply to those that did not pass prior to 2007. Anyone that applies for 2007 and beyond does not do the practical. The instructions state do not download the practical instructions unless told to do so by the ASCP "Chen, Leslie" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 11:42 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] ASCP Exam I'm sorry, I'm confused about the conversations regarding the practical portion of the exam. Are you saying that the practical part is no longer required for HT or HTL cert for ASCP? According to their website, the practical is still required. There are practical instructions: https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el igibility/htl.aspx Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nizarlim <@t> gmail.com Wed Jan 31 15:44:07 2007 From: nizarlim <@t> gmail.com (nizar limaiem) Date: Wed Jan 31 15:44:19 2007 Subject: [Histonet] Help with histo on fish eggs Message-ID: <92805bf10701311344k2e56dc6dw7169a0853532e376@mail.gmail.com> Dear doctor I would thanks you to your rapid replay. I have seen an article in journal of developpement 2001 about the histological study of the developpement of oreochromis niloticus , i am very interesting, so i hope te done a similar work aplied to a fish species dicentrarchus labrax L. in this artical there are no protocol indication. I have applied the protocol witch given me, i rplaced the xylene by toluene because we haven't en our lab. I don't anderstend if the 30 min periode is the time all deshydratation steps (70?,95 and 3x100%) or for each concentration. in my protocol i used 30 min for each concentration. when i use micotome at 5 microns the eggs dont stay in the block as if they are not prfusd by paraffine. the few section abtened colored with H/E showed disordered structure thtcould not be interpreted. so I remplaced the toluene by butanol and 1:1 xylene parafine by ottix parafine (butanol is not missible with paraffine) the section obtened showed clear tissu but not sufficient to be interpeted. thanks in advence I'm afraid may way of expression in nglish are not very good. Sincerely limaiem nizar. Student in marin biotechnology University of biotechnology, Monastir, Tunisia From slappycraw <@t> yahoo.com Wed Jan 31 16:04:41 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Jan 31 16:04:49 2007 Subject: [Histonet] ASCP Exam In-Reply-To: Message-ID: <257081.37475.qm@web53601.mail.yahoo.com> Hate to say it but I think Pathologists have a lot to do with why there isn't mandatory certification for HT and HTL. Jennifer MacDonald wrote: The practical will only apply to those that did not pass prior to 2007. Anyone that applies for 2007 and beyond does not do the practical. The instructions state do not download the practical instructions unless told to do so by the ASCP "Chen, Leslie" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 11:42 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] ASCP Exam I'm sorry, I'm confused about the conversations regarding the practical portion of the exam. Are you saying that the practical part is no longer required for HT or HTL cert for ASCP? According to their website, the practical is still required. There are practical instructions: https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el igibility/htl.aspx Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From shive003 <@t> umn.edu Wed Jan 31 16:08:44 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jan 31 16:08:52 2007 Subject: [Histonet] Minute Virus of Canines IHC Message-ID: <012d01c74584$5fa27370$a1065486@auxs.umn.edu> Does anyone have experience IHC-staining for Minute Virus of Canines (MVC), as opposed to regular canine parvovirus (CPV)? If so, would you share with me your vendor source? Thanks so much in advance. Jan Shivers IHC/Histo/EM Section Head UMN Vet Diag Lab St. Paul, MN 55108 shive003@umn.edu From GMartin <@t> marshallhospital.org Wed Jan 31 16:30:05 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Wed Jan 31 16:28:04 2007 Subject: [Histonet] Paraplast Plus Message-ID: Does any know if embedding wax (Paraplast Plus) can be used in making jewelry by the lost wax method. This would not be used paraffin. We had a flood and several bags of paraffin were damaged ... I don't want to use them for processing. A local high school teacher would like to use them in class to make jewelry. Gary Martin From jnocito <@t> satx.rr.com Wed Jan 31 16:39:14 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 31 16:39:08 2007 Subject: [Histonet] ASCP EXAM References: <1891315748.1170259150324.JavaMail.root@fepweb09> Message-ID: <012101c74588$a36fa420$649eae18@yourxhtr8hvc4p> I was told the reason why they cut out the practical was because it was too expensive to bring everyone to Chicago to score the slides. My question was why doesn't ASCP mail the slides to the people instead of bringing the people to the slides. I was told that everyone had to be in one place. Which leads me to another question. Why not changes the rules? I know, I know. I'm missing the point or something. Is it Friday yet? Joe ----- Original Message ----- From: To: "Cheri Miller" ; "'Ra'" ; ; "Jeanine (CDC/CCID/NCZVED)' 'Bartlett" ; "Kaye Ryan" Sent: Wednesday, January 31, 2007 9:59 AM Subject: RE: [Histonet] ASCP EXAM >I agree with you Kaye. This is the way future techs should be trained. I >have some lab assistants where I currently work who want me to OJT them. I >refuse to because they are not in school or in a histo program. > Just my two cents. > Ron Martin, BS, HT (ASCP) HTL, QIHC > > ---- Kaye Ryan wrote: >> Actually I totally agree with you. I have seen many people that are >> great at memorizing information and great at test taking but cannot for >> the life of them cut a quality section. In our HT program we will NOT >> get rid of the practicum project they must do while in their clinical >> rotations. If they don't pass their project, they do not pass the >> course. I think this change has really put more pressure on the schools >> to make sure we graduate students that know how to cut a decent section >> as well as competent in the areas of troubleshooting. We all hope that >> the schools do attain this goal but being in the profession of so many >> years I know that true troubleshooting abilities come with experience. >> >> Just my thoughts, >> Kaye >> >> Kaye Ryan >> Histology Manager/Educational Coordinator >> Shands Rocky Point Laboratories >> (352) 265-0111, 72093 >> >> >>> "Cheri Miller" 1/31/2007 10:37 AM >>> >> I cant believe they stopped the practicum part of the test. I just took >> it >> a few years back. I would never be the tech I am had I not had to >> practice >> and cut hundreds of slides looking for that perfect one or that >> perfect >> stain, tissue etc. Just my opinion, Cheri >> >> Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Tuesday, January 30, 2007 2:22 PM >> To: Ra; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] ASCP EXAM >> >> Are we discussing the HT or the HTL? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra >> >> Sent: Tue 1/30/2007 3:09 PM >> To: Histonet@lists.utsouthwestern.edu >> Cc: >> Subject: Re: [Histonet] ASCP EXAM >> >> >> >> Funny how that test is so different every time... I found the >> opposite to >> be true. I had some questions on my cert. exam that were word >> for >> word out >> of the ASCP book. I found the NSH series to be more of a >> waste. >> >> Good luck to anyone taking the test! >> Rhonda B. >> >> On 1/29/07, MICHELLE SEAGLE wrote: >> > >> > Don't waste your time or money on the ASCP practical exam >> questions, what >> > a waste. >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jan 31 16:43:36 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 31 16:43:59 2007 Subject: [Histonet] ASCP EXAM References: <831462.68750.qm@web51812.mail.yahoo.com> <358e3b6c0701301209v3250521bu3e8c26ebf78110ab@mail.gmail.com> <000801c7454d$bbc50c80$db01a8c0@plab.local> Message-ID: <012b01c74589$3f4b15f0$649eae18@yourxhtr8hvc4p> which brings me to another question. Before ASCP took away the practical for IHC, you had to have histology experience because they graded not only on staining quality, but cutting quality. Any one who was ASCP registered in any discipline could apply for the IHC. Yeah right. Would have loved to seen an microbiology or a blood bank tech pass that test. I know, I know. Shut up Joe before you get into trouble again. ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Cheri Miller" ; "Ra" ; Sent: Wednesday, January 31, 2007 9:45 AM Subject: RE: [Histonet] ASCP EXAM I agree. I know of one individual with a Master's that will be sitting for her HTL soon and has never done a single special in her life, except for grams in micro. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, January 31, 2007 10:38 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); 'Ra'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM I cant believe they stopped the practicum part of the test. I just took it a few years back. I would never be the tech I am had I not had to practice and cut hundreds of slides looking for that perfect one or that perfect stain, tissue etc. Just my opinion, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, January 30, 2007 2:22 PM To: Ra; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM Are we discussing the HT or the HTL? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra Sent: Tue 1/30/2007 3:09 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] ASCP EXAM Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed Jan 31 16:45:37 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Jan 31 16:45:47 2007 Subject: [Histonet] antibody for E. coli Message-ID: We have a student that is interested in a general antibody that can be used to detect all strains of E. coli. She has samples she wants to screen for E. coli and then she will do more specific IHC staining later. She has expressed an interest in using florescent antibodies. Would this be the way to go? Do you have any suggestions on what antibody she can use for screening? Thanks in advance for the help. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From rjbuesa <@t> yahoo.com Wed Jan 31 16:58:19 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 31 16:58:25 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: Message-ID: <20070131225819.72393.qmail@web61224.mail.yahoo.com> Wax is wax is wax. Ren? J. GMartin@marshallhospital.org wrote: Does any know if embedding wax (Paraplast Plus) can be used in making jewelry by the lost wax method. This would not be used paraffin. We had a flood and several bags of paraffin were damaged ... I don't want to use them for processing. A local high school teacher would like to use them in class to make jewelry. Gary Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. From tkngflght <@t> yahoo.com Wed Jan 31 17:12:11 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Wed Jan 31 17:12:20 2007 Subject: [Histonet] Paraplast Plus/Lost wax In-Reply-To: <20070131225819.72393.qmail@web61224.mail.yahoo.com> References: <20070131225819.72393.qmail@web61224.mail.yahoo.com> Message-ID: <007101c7458d$3d926ca0$6401a8c0@FSDESKTOP> Lost wax methods require the wax 'burn out' completely when heated prior to casting. With the plastics in the wax--there might be a residue. Only one way to find out--try it! Cheryl Full Staff Inc 281.852.9457 From caron_fournier <@t> yahoo.ca Wed Jan 31 17:59:39 2007 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Wed Jan 31 17:59:46 2007 Subject: [Histonet] re: PMMA removal Message-ID: <17743.75358.qm@web35414.mail.mud.yahoo.com> Does anyone have a method for removing polymerized PMMA from bone and not harming the bone? Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jnocito <@t> satx.rr.com Wed Jan 31 19:04:27 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 31 19:04:08 2007 Subject: [Histonet] ASCP Exam References: <257081.37475.qm@web53601.mail.yahoo.com> Message-ID: <002d01c7459c$ec97e220$649eae18@yourxhtr8hvc4p> of course. They don't want to pay the price. It always comes down to $$$$$$ Joe ----- Original Message ----- From: "Larry Woody" To: "Jennifer MacDonald" ; "Chen, Leslie" Cc: ; Sent: Wednesday, January 31, 2007 4:04 PM Subject: Re: [Histonet] ASCP Exam > Hate to say it but I think Pathologists have a lot to do with why there > isn't mandatory certification for HT and HTL. > > Jennifer MacDonald wrote: The practical will only > apply to those that did not pass prior to 2007. > Anyone that applies for 2007 and beyond does not do the practical. > The instructions state do not download the practical instructions unless > told to do so by the ASCP > > > > > > > "Chen, Leslie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/31/2007 11:42 AM > > To > "'histonet@lists.utsouthwestern.edu'" > cc > > Subject > [Histonet] ASCP Exam > > > > > > > I'm sorry, I'm confused about the conversations regarding the practical > portion of the exam. Are you saying that the practical part is no longer > required for HT or HTL cert for ASCP? According to their website, the > practical is still required. There are practical instructions: > https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el > > igibility/htl.aspx > Thanks. > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. If you are not the intended > recipient, please immediately notify us by return email, and delete this > message from your computer. > ---------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Don't be flakey. Get Yahoo! Mail for Mobile and > always stay connected to friends. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cytologer <@t> msn.com Wed Jan 31 19:06:57 2007 From: cytologer <@t> msn.com (Choi Ul Soo) Date: Wed Jan 31 19:07:14 2007 Subject: [Histonet] anti-bombesion antibody for dog tissue Message-ID: Hi histonetters, Thank you for your precious replies to my 'parafffin embedded section for TEM anaylsis' question! I am trying to do IHC on canine tissues with anti bombesin antibody. Does anyone out there have information on anti-bombesin antibody appliable to dog tissue? Let me hear your experiences on this antibody. Thank you. Ulsoo Choi DVM, PhD VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742 Tel. 82-02-880-8688 Mobile. 82-016-9228-8634 Fax. 82-02-880-8662 _________________________________________________________________ ?? ???? ??? MSN SMS ???? ?????. http://im.msn.co.kr/new/function/function_01_02.asp From JMacDonald <@t> mtsac.edu Wed Jan 31 19:15:38 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 31 19:15:46 2007 Subject: [Histonet] ASCP Exam In-Reply-To: <257081.37475.qm@web53601.mail.yahoo.com> Message-ID: Lack of a practical has nothing to do with mandatory certification. The practical was a component of the HT/HTL (ASCP) certification exam. Passing of the exam gives the applicant national certification with the ASCP. Mandating certification may very well be dictated by pathologists, but it has ABSOLUTELY NOTHING to do with the practical aspect of the HT/HTL exam being discontinued. It is interesting to note that no other laboratory science certification requires a practical. Does that make those certified applicants any less competent? Expense was not the only criteria that was used to make the decision to discontinue the practical. There were many factors. With HIIPA regulations obtaining the tissue was also becoming difficult for applicants. Some applicants have access to fully automated labs, while others don't. Some out there are unethical and don't do any of the work themselves. What makes it a fair and valid exam. At least with the "written" portion applicants must show knowledge of the principles and procedures and the ability to recognize problems and to troubleshoot them. Sending the slides to the graders is not an option. The ASCP had a very sophisticated grading system to ensure anonymity to each applicant and to ensure that the grading was equitable. Sending out slides to graders would have compromised a fair system. You also open up the possibility of breakage and lost slides/blocks. There is no method of examination that will please everyone. The ASCP, who had feedback from many people in the histology field, made the decision that they felt would be fair to MOST applicants. The ability to cut good sections does not make one a good histotech, just a good microtomist. Just as the ability to pass the computer portion of the exam does not make one a good histotech, but the odds are better for a well rounded employee. One needs to understand and interpret the stains they are performing, not just load them on the Stainer. Just my opinion, Jennifer MacDonald Larry Woody 01/31/2007 02:04 PM To Jennifer MacDonald , "Chen, Leslie" cc "'histonet@lists.utsouthwestern.edu'" , histonet-bounces@lists.utsouthwestern.edu Subject Re: [Histonet] ASCP Exam Hate to say it but I think Pathologists have a lot to do with why there isn't mandatory certification for HT and HTL. Jennifer MacDonald wrote: The practical will only apply to those that did not pass prior to 2007. Anyone that applies for 2007 and beyond does not do the practical. The instructions state do not download the practical instructions unless told to do so by the ASCP "Chen, Leslie" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2007 11:42 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] ASCP Exam I'm sorry, I'm confused about the conversations regarding the practical portion of the exam. Are you saying that the practical part is no longer required for HT or HTL cert for ASCP? According to their website, the practical is still required. There are practical instructions: https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el igibility/htl.aspx Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From jnocito <@t> satx.rr.com Wed Jan 31 19:47:18 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 31 19:47:02 2007 Subject: [Histonet] ASCP Exam References: Message-ID: <004e01c745a2$eaf016d0$649eae18@yourxhtr8hvc4p> It is Friday!!!!! Jennifer, I must respectfully disagree with you. I have two techs that obtained tissue from the same autopsy, processed, embedded, stained and coverslipped said tissue at the same time. I reviewed the slides as did my medical director. The techs were getting mad at me because I kept kicking slides back for one reason or another. One passed the practical, one didn't. I still don't understand how that happened. In essence, who ever graded those slides said that my pathologist and I didn't know what we were doing. Now, on the other hand, all prospective employees that apply at my lab sit down and cut a few blocks to demonstrate that they know how to handle a microtome. Book knowledge is one thing, but you have to agree in the histo lab, skill is very important. Just my 3 cents. Joe "let the flaming begin, again" BS, PA, HT(ASCP)QIHC ----- Original Message ----- From: "Jennifer MacDonald" To: "Larry Woody" Cc: ; ; "Chen, Leslie" Sent: Wednesday, January 31, 2007 7:15 PM Subject: Re: [Histonet] ASCP Exam > Lack of a practical has nothing to do with mandatory certification. The > practical was a component of the HT/HTL (ASCP) certification exam. Passing > of the exam gives the applicant national certification with the ASCP. > Mandating certification may very well be dictated by pathologists, but it > has ABSOLUTELY NOTHING to do with the practical aspect of the HT/HTL exam > being discontinued. > It is interesting to note that no other laboratory science certification > requires a practical. Does that make those certified applicants any less > competent? > Expense was not the only criteria that was used to make the decision to > discontinue the practical. There were many factors. With HIIPA > regulations obtaining the tissue was also becoming difficult for > applicants. Some applicants have access to fully automated labs, while > others don't. Some out there are unethical and don't do any of the work > themselves. What makes it a fair and valid exam. At least with the > "written" portion applicants must show knowledge of the principles and > procedures and the ability to recognize problems and to troubleshoot them. > > Sending the slides to the graders is not an option. The ASCP had a very > sophisticated grading system to ensure anonymity to each applicant and to > ensure that the grading was equitable. Sending out slides to graders > would have compromised a fair system. You also open up the possibility of > breakage and lost slides/blocks. > There is no method of examination that will please everyone. The ASCP, > who had feedback from many people in the histology field, made the > decision that they felt would be fair to MOST applicants. > The ability to cut good sections does not make one a good histotech, just > a good microtomist. Just as the ability to pass the computer portion of > the exam does not make one a good histotech, but the odds are better for a > well rounded employee. One needs to understand and interpret the stains > they are performing, not just load them on the Stainer. > Just my opinion, > Jennifer MacDonald > > > > > Larry Woody > 01/31/2007 02:04 PM > > To > Jennifer MacDonald , "Chen, Leslie" > > cc > "'histonet@lists.utsouthwestern.edu'" , > histonet-bounces@lists.utsouthwestern.edu > Subject > Re: [Histonet] ASCP Exam > > > > > > > Hate to say it but I think Pathologists have a lot to do with why there > isn't mandatory certification for HT and HTL. > > Jennifer MacDonald wrote: > The practical will only apply to those that did not pass prior to 2007. > Anyone that applies for 2007 and beyond does not do the practical. > The instructions state do not download the practical instructions unless > told to do so by the ASCP > > > > > > > "Chen, Leslie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/31/2007 11:42 AM > > To > "'histonet@lists.utsouthwestern.edu'" > cc > > Subject > [Histonet] ASCP Exam > > > > > > > I'm sorry, I'm confused about the conversations regarding the practical > portion of the exam. Are you saying that the practical part is no longer > required for HT or HTL cert for ASCP? According to their website, the > practical is still required. There are practical instructions: > https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el > > > igibility/htl.aspx > Thanks. > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. If you are not the intended > recipient, please immediately notify us by return email, and delete this > message from your computer. > ---------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Don't be flakey. Get Yahoo! Mail for Mobile and > always stay connected to friends. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Jan 31 19:56:56 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Jan 31 19:57:20 2007 Subject: [Histonet] Re: Paper biopsy bags also called "tea bags" Message-ID: Vikki Baker asks: >>I'm looking to buy the paper biopsy bags for our smaller tissues. We used to get them from Fisher, but they have discontinued it. Is anyone out there getting these still?<< These are actually made of a porous nylon mesh. There's more than one size, and you want the smallest. It's handy to have a small plastic funnel to pour the fixative and the specimen through the bag. The mesh is very fine, and almost nothing escapes from it. These were available from Shandon about six years ago. I guess you can still get them from whatever Shandon is called this week. I don't have a catalog. They're called "tea bags" because a lot of people used to buy empty tea bags to enclose small specimens, more convenient for both the grosser and the embedder than the traditional lens paper. Tea bags are made out of very long-staple abaca fiber. I think they're obsolete in the histologiy lab, and I don't know whether they're still available at all. I've recently acquired an elderly locum tenens client (sheesh, he's only a year and a half younger than ME) who still insists on using lens paper. Bob Richmond Samurai Pathologist Knoxville TN From JMacDonald <@t> mtsac.edu Wed Jan 31 20:05:45 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 31 20:05:53 2007 Subject: [Histonet] ASCP Exam In-Reply-To: <004e01c745a2$eaf016d0$649eae18@yourxhtr8hvc4p> Message-ID: Joe, I agree with you that skill is very important to be a competent histotech. What I disagree with is that the ASCP practical exam was a fair measure of the competence of the skill of the histotech. Your employees' situation is a classic example. Do you feel that one was more competent than the other? Was one set a little better than the other? I also know of an example where a tech with more than 25 years experience did the practical for an individual and the applicant failed. Time on the bench is also not a good measure of skill. I have graded slides at the ASCP and I have to tell you that it is very difficult to give a failing grade. Others must agree with you before you can deduct "big" points. Also no one person grades a set of slides. A minimum of three graders will see the slides. How close were the scores, if you don't mind me asking? When grading the slides the graders are not looking for slides of diagnostic value, they are looking for perfect. Right or wrong that was the standard that every applicant was held to. As I said, there is no perfect way to test the competence of applicants. In my opinion the practical needed to go. That is not to say that when interviewing job applicants you cannot ask them to prove their microtomy skills. In my experience if an applicant has a strong working knowledge of the theory and some microtomy experience it is easier to train them than it is to train someone with only microtomy skills. It also depends on what you want them to accomplish in the lab. If you strictly need a good microtomist than really good microtomy skills are necessary. If you need someone to be able to troubleshoot staining problems the good microtomist would not be the person for the job. That does not make one a better tech than the other. It all depends on the needs and priorities of the situation. Jennifer "Joe Nocito" 01/31/2007 05:47 PM To "Jennifer MacDonald" , "Larry Woody" cc , , "Chen, Leslie" Subject Re: [Histonet] ASCP Exam It is Friday!!!!! Jennifer, I must respectfully disagree with you. I have two techs that obtained tissue from the same autopsy, processed, embedded, stained and coverslipped said tissue at the same time. I reviewed the slides as did my medical director. The techs were getting mad at me because I kept kicking slides back for one reason or another. One passed the practical, one didn't. I still don't understand how that happened. In essence, who ever graded those slides said that my pathologist and I didn't know what we were doing. Now, on the other hand, all prospective employees that apply at my lab sit down and cut a few blocks to demonstrate that they know how to handle a microtome. Book knowledge is one thing, but you have to agree in the histo lab, skill is very important. Just my 3 cents. Joe "let the flaming begin, again" BS, PA, HT(ASCP)QIHC ----- Original Message ----- From: "Jennifer MacDonald" To: "Larry Woody" Cc: ; ; "Chen, Leslie" Sent: Wednesday, January 31, 2007 7:15 PM Subject: Re: [Histonet] ASCP Exam > Lack of a practical has nothing to do with mandatory certification. The > practical was a component of the HT/HTL (ASCP) certification exam. Passing > of the exam gives the applicant national certification with the ASCP. > Mandating certification may very well be dictated by pathologists, but it > has ABSOLUTELY NOTHING to do with the practical aspect of the HT/HTL exam > being discontinued. > It is interesting to note that no other laboratory science certification > requires a practical. Does that make those certified applicants any less > competent? > Expense was not the only criteria that was used to make the decision to > discontinue the practical. There were many factors. With HIIPA > regulations obtaining the tissue was also becoming difficult for > applicants. Some applicants have access to fully automated labs, while > others don't. Some out there are unethical and don't do any of the work > themselves. What makes it a fair and valid exam. At least with the > "written" portion applicants must show knowledge of the principles and > procedures and the ability to recognize problems and to troubleshoot them. > > Sending the slides to the graders is not an option. The ASCP had a very > sophisticated grading system to ensure anonymity to each applicant and to > ensure that the grading was equitable. Sending out slides to graders > would have compromised a fair system. You also open up the possibility of > breakage and lost slides/blocks. > There is no method of examination that will please everyone. The ASCP, > who had feedback from many people in the histology field, made the > decision that they felt would be fair to MOST applicants. > The ability to cut good sections does not make one a good histotech, just > a good microtomist. Just as the ability to pass the computer portion of > the exam does not make one a good histotech, but the odds are better for a > well rounded employee. One needs to understand and interpret the stains > they are performing, not just load them on the Stainer. > Just my opinion, > Jennifer MacDonald > > > > > Larry Woody > 01/31/2007 02:04 PM > > To > Jennifer MacDonald , "Chen, Leslie" > > cc > "'histonet@lists.utsouthwestern.edu'" , > histonet-bounces@lists.utsouthwestern.edu > Subject > Re: [Histonet] ASCP Exam > > > > > > > Hate to say it but I think Pathologists have a lot to do with why there > isn't mandatory certification for HT and HTL. > > Jennifer MacDonald wrote: > The practical will only apply to those that did not pass prior to 2007. > Anyone that applies for 2007 and beyond does not do the practical. > The instructions state do not download the practical instructions unless > told to do so by the ASCP > > > > > > > "Chen, Leslie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/31/2007 11:42 AM > > To > "'histonet@lists.utsouthwestern.edu'" > cc > > Subject > [Histonet] ASCP Exam > > > > > > > I'm sorry, I'm confused about the conversations regarding the practical > portion of the exam. Are you saying that the practical part is no longer > required for HT or HTL cert for ASCP? According to their website, the > practical is still required. There are practical instructions: > https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el > > > igibility/htl.aspx > Thanks. > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. If you are not the intended > recipient, please immediately notify us by return email, and delete this > message from your computer. > ---------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Don't be flakey. Get Yahoo! Mail for Mobile and > always stay connected to friends. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Jan 31 21:07:47 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Jan 31 21:07:55 2007 Subject: [Histonet] ASCP Exam In-Reply-To: Message-ID: <790816.10876.qm@web53613.mail.yahoo.com> Academics and skill, what a combination of talent yet so elusive in pathology labs across the country. Getting rid of the practical will make no difference in the number of people going into the field and gets us no closer to what we really need.$$$$$$ and yes Ray it's me and I use my home address for the Histonet because as you know I'm much too busy at Amgen . Jennifer MacDonald wrote: Joe, I agree with you that skill is very important to be a competent histotech. What I disagree with is that the ASCP practical exam was a fair measure of the competence of the skill of the histotech. Your employees' situation is a classic example. Do you feel that one was more competent than the other? Was one set a little better than the other? I also know of an example where a tech with more than 25 years experience did the practical for an individual and the applicant failed. Time on the bench is also not a good measure of skill. I have graded slides at the ASCP and I have to tell you that it is very difficult to give a failing grade. Others must agree with you before you can deduct "big" points. Also no one person grades a set of slides. A minimum of three graders will see the slides. How close were the scores, if you don't mind me asking? When grading the slides the graders are not looking for slides of diagnostic value, they are looking for perfect. Right or wrong that was the standard that every applicant was held to. As I said, there is no perfect way to test the competence of applicants. In my opinion the practical needed to go. That is not to say that when interviewing job applicants you cannot ask them to prove their microtomy skills. In my experience if an applicant has a strong working knowledge of the theory and some microtomy experience it is easier to train them than it is to train someone with only microtomy skills. It also depends on what you want them to accomplish in the lab. If you strictly need a good microtomist than really good microtomy skills are necessary. If you need someone to be able to troubleshoot staining problems the good microtomist would not be the person for the job. That does not make one a better tech than the other. It all depends on the needs and priorities of the situation. Jennifer "Joe Nocito" 01/31/2007 05:47 PM To "Jennifer MacDonald" , "Larry Woody" cc , , "Chen, Leslie" Subject Re: [Histonet] ASCP Exam It is Friday!!!!! Jennifer, I must respectfully disagree with you. I have two techs that obtained tissue from the same autopsy, processed, embedded, stained and coverslipped said tissue at the same time. I reviewed the slides as did my medical director. The techs were getting mad at me because I kept kicking slides back for one reason or another. One passed the practical, one didn't. I still don't understand how that happened. In essence, who ever graded those slides said that my pathologist and I didn't know what we were doing. Now, on the other hand, all prospective employees that apply at my lab sit down and cut a few blocks to demonstrate that they know how to handle a microtome. Book knowledge is one thing, but you have to agree in the histo lab, skill is very important. Just my 3 cents. Joe "let the flaming begin, again" BS, PA, HT(ASCP)QIHC ----- Original Message ----- From: "Jennifer MacDonald" To: "Larry Woody" Cc: ; ; "Chen, Leslie" Sent: Wednesday, January 31, 2007 7:15 PM Subject: Re: [Histonet] ASCP Exam > Lack of a practical has nothing to do with mandatory certification. The > practical was a component of the HT/HTL (ASCP) certification exam. Passing > of the exam gives the applicant national certification with the ASCP. > Mandating certification may very well be dictated by pathologists, but it > has ABSOLUTELY NOTHING to do with the practical aspect of the HT/HTL exam > being discontinued. > It is interesting to note that no other laboratory science certification > requires a practical. Does that make those certified applicants any less > competent? > Expense was not the only criteria that was used to make the decision to > discontinue the practical. There were many factors. With HIIPA > regulations obtaining the tissue was also becoming difficult for > applicants. Some applicants have access to fully automated labs, while > others don't. Some out there are unethical and don't do any of the work > themselves. What makes it a fair and valid exam. At least with the > "written" portion applicants must show knowledge of the principles and > procedures and the ability to recognize problems and to troubleshoot them. > > Sending the slides to the graders is not an option. The ASCP had a very > sophisticated grading system to ensure anonymity to each applicant and to > ensure that the grading was equitable. Sending out slides to graders > would have compromised a fair system. You also open up the possibility of > breakage and lost slides/blocks. > There is no method of examination that will please everyone. The ASCP, > who had feedback from many people in the histology field, made the > decision that they felt would be fair to MOST applicants. > The ability to cut good sections does not make one a good histotech, just > a good microtomist. Just as the ability to pass the computer portion of > the exam does not make one a good histotech, but the odds are better for a > well rounded employee. One needs to understand and interpret the stains > they are performing, not just load them on the Stainer. > Just my opinion, > Jennifer MacDonald > > > > > Larry Woody > 01/31/2007 02:04 PM > > To > Jennifer MacDonald , "Chen, Leslie" > > cc > "'histonet@lists.utsouthwestern.edu'" , > histonet-bounces@lists.utsouthwestern.edu > Subject > Re: [Histonet] ASCP Exam > > > > > > > Hate to say it but I think Pathologists have a lot to do with why there > isn't mandatory certification for HT and HTL. > > Jennifer MacDonald wrote: > The practical will only apply to those that did not pass prior to 2007. > Anyone that applies for 2007 and beyond does not do the practical. > The instructions state do not download the practical instructions unless > told to do so by the ASCP > > > > > > > "Chen, Leslie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/31/2007 11:42 AM > > To > "'histonet@lists.utsouthwestern.edu'" > cc > > Subject > [Histonet] ASCP Exam > > > > > > > I'm sorry, I'm confused about the conversations regarding the practical > portion of the exam. Are you saying that the practical part is no longer > required for HT or HTL cert for ASCP? According to their website, the > practical is still required. There are practical instructions: > https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el > > > igibility/htl.aspx > Thanks. > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. 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