[Histonet] formalin fixed mouse ears-help

[Yaskovich, Ruth A (NIH/NIDCR) [E]]

Mary,
If you can give them a decal soak. I use Immunocal. I keep playing with
the water bath temperature and then take each section one at a time.
Mine are not splitting at all but they are fixed in 4% Para. (which I
don't think matters for the cutting)
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Crainiofacial Research
Neurobiology and Pain Therapeutics Branch

-----Original Message-----
From: Mary Beauchamp [mailto:mbeaucha <@t> benaroyaresearch.org] 
Sent: Thursday, February 08, 2007 5:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] formalin fixed mouse ears-help

Hi there,

 

I'm having problems with 10% neutral buffered formalin fixed mouse ears
embedded in paraffin. They are splitting from the cartilage as soon, and
I mean, as soon as they hit the water bath.

 

I'm open to any suggestions, no matter how silly or simple. This is a
chronic problem and these mouse ears will continue to be submitted to me
for a long time.

 

Please help!

 

-Mary B


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