From lpwenk <@t> sbcglobal.net Thu Feb 1 05:18:28 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Feb 1 05:18:49 2007 Subject: [Histonet] ASCP EXAM In-Reply-To: <000801c7454d$bbc50c80$db01a8c0@plab.local> Message-ID: <002401c745f2$b375c330$83e72d4b@HPPav2> I'm going to wade in a little. Jennifer MacDonald has done a great job with a lot of the information. (Thanks, Jennifer) So, I'm just going to make a couple of comments about several questions that have been brought up by various people. No, someone can't get a degree, take the test, then apply for a job. For both HT and HTL, they need to get the degree (AA/AS for HT, BA/BS for HTL), get 1 year's full-time experience in histotechnology, THEN they can take the exam. Yes, labs will hire people with the degree and train them on-the-job, OJT. Most institutions have to - there aren't enough NAACLS accredited HT and HTL schools in the country. Open and active, there are 24 HT and 3 HTL programs in the US. That's not enough. So the majority are still OJT, with great variation as to level of training and exposure to different tissues and stains. Correct, no other lab discipline has to "prove" they can "do" the job with a practical. Microbiologists don't have to plate, phlebotomists don't have to draw blood, etc. They rely on a written exam only. And now HT/HTL does also. Passing - if you think about it, there are only 4 different results: - Pass both written and practical - Fail both written and practical - Pass written, fail practical - Pass practical, fail written The one category that had the LEAST people was passing the written and failing the practical. Most of the time, either the candidate knew how to do the practical AND they knew the theory (pass both), or they didn't know either (fail both). Then there was the group of people who could do the work, but didn't know what they were doing or why, or how to problem solve/troubleshoot when things went wrong (pass practical/fail written). Very few people would "know the theory" and pass the written, but then not know how to do the stains or cut (fail the practical). Usually, if they never did staining, they didn't know what was in the stain or how to troubleshoot, so they usually didn't pass the written either. So the deciding factor turned out to be passing the written part. If candidates could pass the written, they knew the theory of staining and troubleshooting microtomy issues, and this usually translated over to doing a good job on the practical, so they usually passed. If someone didn't know theory, they usually didn't know what a good stain looked like or what good tissue looked like, so they failed both the written and the practical. Of course, there are going to be people responding to this who know someone who is an example of just the opposite of what I just said. No test - written or practical - is a 100% determiner. After all, I passed physics, and I still don't understand electricity - theory or practice. Also - there is a part of the HT/HTL application where the person attests, and so does their supervisor/pathologist, that the candidate has experience in ALL aspects of histotechnology - fixation, processing, microtomy, special stains. And they have to document their experience. Yes, I know, they can lie. But these are the same people and institutions that would have someone else do the stains and sectioning on the practical exam. But with the new format of relying just on a written exam, the candidate won't be passing the practical that someone else did. They have to pass a written exam taken under very controlled circumstances - 2 ID's, fingerprint, etc. So the person taking the exam really is that person. One last thing - in my program, I'm still having the students turn in a set of H&E's and special stains, similar to the ASCP exam. About 25 different tissues with H&E's and about 10 different special stains (they actually learn about 50 special stains with me, but they only have to do a practical of 10 special stain). And I still grade these slides - and they better be great. This is their last "final" from me - they have to prove that they do know what good microtomy is, what a great H&E looks like, what good special stains look like. From what I hear, most accredited HT and HTL programs do something similar. So hiring a NAACLS accredited graduate should get you someone with experience and knowledge in sectioning and special stains. (Of course, there is still personality issues and different levels of ability - but that's the same as someone learning OJT.) So what we need are more NAACLS-accredited HT and HTL programs in each state - in my humble opionion. Peggy Wenk -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, January 31, 2007 10:38 AM To: 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Ra'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM I cant believe they stopped the practicum part of the test. I just took it a few years back. I would never be the tech I am had I not had to practice and cut hundreds of slides looking for that perfect one or that perfect stain, tissue etc. Just my opinion, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, January 30, 2007 2:22 PM To: Ra; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP EXAM Are we discussing the HT or the HTL? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ra Sent: Tue 1/30/2007 3:09 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] ASCP EXAM Funny how that test is so different every time... I found the opposite to be true. I had some questions on my cert. exam that were word for word out of the ASCP book. I found the NSH series to be more of a waste. Good luck to anyone taking the test! Rhonda B. On 1/29/07, MICHELLE SEAGLE wrote: > > Don't waste your time or money on the ASCP practical exam questions, what > a waste. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Thu Feb 1 06:30:43 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Feb 1 06:30:53 2007 Subject: [Histonet] Michel's transport medium Message-ID: Betty, We purchase our Michel's transport media from Newcomer supply. I would imagine they could ship to China! Anyway maybe they can give you some tech tips. www.newcomersupply.com Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 . Message: 13 Date: Wed, 31 Jan 2007 20:40:01 +0800 (CST) From: "=?gb2312?B?1qO0us+8?=" Subject: [Histonet] Michel's transport medium To: histonet@lists.utsouthwestern.edu Message-ID: <45C08E21.000005.18600@bj126app12.126.com> Content-Type: text/plain; charset="gb2312" Hello, everyone. I am going to use Michel's transport medium to send renal tissue samples. I got a protocol on the medium preparations(see belows).. When I made the preparation according to the protocol, I met troubles. At the first step(1.0 M potassium citrate buffer pH 7.0), I could not adjust pH using the 1M potassium hydroxide. I had to adjust pH to 7.0 using 10M potassium hydroxide(40ml). And then, I could not dissolve 55 grams of ammonium sulfate in 100 ml washing solution. About half of ammonium sulfate(made in China) kept unsolved, even though I incubated it in heat water and/or mechanical stirring. When I bought a bottle of new ammonium sulfate, it still did not work. Later, I tried to dissolve 55g ammonium sulfate in 100ml distilled water, but I failed. I tried and thought it again and again. I did not know what is the matter. Please tell me what I should do. I am waiting for your kindly reply. Sincerely, Betty Nanjing University School of Medicine, Research institute of Nephrology, Jinling Hospital, Nanjing, China 210002 Tel: 86-025-81765894 Fax: 86-025-84801992 From sheila_adey <@t> hotmail.com Thu Feb 1 08:19:01 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Feb 1 08:19:15 2007 Subject: [Histonet] Re: Paper biopsy bags also called "tea bags" In-Reply-To: Message-ID: We just started purchasing these biopsy bags from Mercedes medical. Their price was almost half of what our other quotes were. Sheila Adey HT MLT Port Huron Hospital Michigan >From: RSRICHMOND@aol.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Paper biopsy bags also called "tea bags" >Date: Wed, 31 Jan 2007 20:56:56 EST > >Vikki Baker asks: > >>I'm looking to buy the paper biopsy bags for our smaller tissues. We >used >to get them from Fisher, but they have discontinued it. Is anyone out there >getting these still?<< > >These are actually made of a porous nylon mesh. There's more than one size, >and you want the smallest. It's handy to have a small plastic funnel to >pour >the fixative and the specimen through the bag. The mesh is very fine, and >almost >nothing escapes from it. > >These were available from Shandon about six years ago. I guess you can >still >get them from whatever Shandon is called this week. I don't have a catalog. > >They're called "tea bags" because a lot of people used to buy empty tea >bags >to enclose small specimens, more convenient for both the grosser and the >embedder than the traditional lens paper. Tea bags are made out of very >long-staple >abaca fiber. I think they're obsolete in the histologiy lab, and I don't >know >whether they're still available at all. > >I've recently acquired an elderly locum tenens client (sheesh, he's only a >year and a half younger than ME) who still insists on using lens paper. > >Bob Richmond >Samurai Pathologist >Knoxville TN >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA From tbraud <@t> holyredeemer.com Thu Feb 1 08:32:50 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Feb 1 08:33:05 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: <20070131225819.72393.qmail@web61224.mail.yahoo.com> Message-ID: I'm responding to this from 2 angles. One, as a Histotech with 30 years exp, and the other as a scupture in Bronze, using the lost wax method for almost as long. DO NOT!!!!! use paraplast plus, or any other Histology wax for lost wax casting. The additional components in the Histology wax are entirely unsuitable for the high temps used to mold, carve and otherwise form the wax piece. My suggestion for the teacher is to go to the Dick Blick Art Catalogue and order a large supply of French Wax (the red one) which is formulated for that specific use. 25lbs, is very very cheap, and poses no risks to use at high temps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, January 31, 2007 5:58 PM To: GMartin@marshallhospital.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Plus Wax is wax is wax. Ren? J. GMartin@marshallhospital.org wrote: Does any know if embedding wax (Paraplast Plus) can be used in making jewelry by the lost wax method. This would not be used paraffin. We had a flood and several bags of paraffin were damaged ... I don't want to use them for processing. A local high school teacher would like to use them in class to make jewelry. Gary Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From pmarcum <@t> vet.upenn.edu Thu Feb 1 08:46:21 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Feb 1 08:46:32 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: References: <20070131225819.72393.qmail@web61224.mail.yahoo.com> Message-ID: <6.2.5.6.2.20070201094459.01c5c398@vet.upenn.edu> I agree with Terri. Paraplus Plus contains DMSO and is for recommended for reuse. Pam At 09:32 AM 2/1/2007, Terri Braud wrote: >I'm responding to this from 2 angles. >One, as a Histotech with 30 years exp, and the >other as a scupture in Bronze, using the lost wax method for almost as long. >DO NOT!!!!! use paraplast plus, or any other >Histology wax for lost wax casting. The >additional components in the Histology wax are >entirely unsuitable for the high temps used to >mold, carve and otherwise form the wax piece. >My suggestion for the teacher is to go to the >Dick Blick Art Catalogue and order a large >supply of French Wax (the red one) which is >formulated for that specific use. 25lbs, is >very very cheap, and poses no risks to use at high temps. >Terri L. Braud, HT(ASCP) >Anatomic Pathology Supv. >Laboratory, Holy Redeemer Hospital >1648 Huntingdon Pike >Meadowbrook, PA 19046 >(215) 938-3689 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Wednesday, January 31, 2007 5:58 PM >To: GMartin@marshallhospital.org; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Paraplast Plus > > >Wax is wax is wax. > Ren? J. > >GMartin@marshallhospital.org wrote: > Does any know if embedding wax (Paraplast Plus) can be used in making >jewelry by the lost wax method. This would not be used paraffin. We had a >flood and several bags of paraffin were damaged ... I don't want to use >them for processing. A local high school teacher would like to use them in >class to make jewelry. >Gary Martin > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Food fight? Enjoy some healthy debate >in the Yahoo! Answers Food & Drink Q&A. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to which >it is addressed. It may contain information that >is privileged and/or confidential, >and the use or disclosure of such information >may also be restricted under applicable >federal and state law. If you received this >communication in error, please do not >distribute any part of it or retain any copies, >and delete the original E-Mail. >Please notify the sender of any error by E-Mail >at the electronic address shown. > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From froyer <@t> bitstream.net Thu Feb 1 08:48:16 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Feb 1 08:48:53 2007 Subject: [Histonet] Paraplast Plus/Lost wax In-Reply-To: <007101c7458d$3d926ca0$6401a8c0@FSDESKTOP> References: <20070131225819.72393.qmail@web61224.mail.yahoo.com> <007101c7458d$3d926ca0$6401a8c0@FSDESKTOP> Message-ID: <003801c74610$0263e270$7701a80a@Ford> My first thought was the same as Cheryl's that there may be unwanted residue (from the polymers & plastics) left over from the "burn out". Also, the wax used in the "Lost Wax" method is normally a combination of pure paraffin and bees wax in a ratio of about 60/40. But as Cheryl suggests - try it out on a practice piece and see what happens. If it works with no residue, I would then recommend that the instructor procure some bees wax (available at all craft stores) and add that to their Paraplast for even better results. They may find that Paraplast alone is too brittle to handle when harden. The bees wax makes the mixture more malleable for easier finishing touch-ups before final molding. If the Paraplast is found unsuitable due to the aforementioned concerns, then there are other things that the school could use it for. I donated some old embedding media to my kid's school to make candles and fire starters that they sold at a school-wide crafts fair as a fund raiser. Contact me "Off List" for more detail if interested. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Kerry Sent: Wednesday, January 31, 2007 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraplast Plus/Lost wax Lost wax methods require the wax 'burn out' completely when heated prior to casting. With the plastics in the wax--there might be a residue. Only one way to find out--try it! Cheryl Full Staff Inc 281.852.9457 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Feb 1 09:02:37 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Feb 1 09:03:20 2007 Subject: [Histonet] Paraplast Plus/Lost wax In-Reply-To: <003801c74610$0263e270$7701a80a@Ford> Message-ID: And if the kids start to taste almonds while working with the DMSO, they should probably stop. "Ford Royer" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/01/2007 08:48 AM To cc Subject RE: [Histonet] Paraplast Plus/Lost wax My first thought was the same as Cheryl's that there may be unwanted residue (from the polymers & plastics) left over from the "burn out". Also, the wax used in the "Lost Wax" method is normally a combination of pure paraffin and bees wax in a ratio of about 60/40. But as Cheryl suggests - try it out on a practice piece and see what happens. If it works with no residue, I would then recommend that the instructor procure some bees wax (available at all craft stores) and add that to their Paraplast for even better results. They may find that Paraplast alone is too brittle to handle when harden. The bees wax makes the mixture more malleable for easier finishing touch-ups before final molding. If the Paraplast is found unsuitable due to the aforementioned concerns, then there are other things that the school could use it for. I donated some old embedding media to my kid's school to make candles and fire starters that they sold at a school-wide crafts fair as a fund raiser. Contact me "Off List" for more detail if interested. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Kerry Sent: Wednesday, January 31, 2007 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraplast Plus/Lost wax Lost wax methods require the wax 'burn out' completely when heated prior to casting. With the plastics in the wax--there might be a residue. Only one way to find out--try it! Cheryl Full Staff Inc 281.852.9457 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Feb 1 09:05:54 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Feb 1 09:06:37 2007 Subject: [Histonet] Paraplast Plus/Lost wax In-Reply-To: <003801c74610$0263e270$7701a80a@Ford> Message-ID: Oh- sorry, that's a strong garlic taste - -almond taste is cyanide, I think. I've only tasted the DMSO when working with my horse. I wear gloves now. Never really tasted cyanide. From pmarcum <@t> vet.upenn.edu Thu Feb 1 09:15:46 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Feb 1 09:16:24 2007 Subject: [Histonet] Paraplast Plus/Lost wax In-Reply-To: References: <003801c74610$0263e270$7701a80a@Ford> Message-ID: <6.2.5.6.2.20070201101532.01c5bb40@vet.upenn.edu> Tastes like garlic At 10:05 AM 2/1/2007, Jackie M O'Connor wrote: >Oh- sorry, that's a strong garlic taste - -almond taste is cyanide, I >think. I've only tasted the DMSO when working with my horse. I wear >gloves now. Never really tasted cyanide. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From tbraud <@t> holyredeemer.com Thu Feb 1 09:32:45 2007 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Feb 1 09:32:58 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: Message-ID: "as doing sculpture" and "2.5 pounds" my spelling sucks, and spellcheck? pffffftttttt...... the teacher also might be able to procure some french wax from a local jeweler in small quantities -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Terri Braud Sent: Thursday, February 01, 2007 9:33 AM To: Histonet (E-mail) Subject: RE: [Histonet] Paraplast Plus I'm responding to this from 2 angles. One, as a Histotech with 30 years exp, and the other as a scupture in Bronze, using the lost wax method for almost as long. DO NOT!!!!! use paraplast plus, or any other Histology wax for lost wax casting. The additional components in the Histology wax are entirely unsuitable for the high temps used to mold, carve and otherwise form the wax piece. My suggestion for the teacher is to go to the Dick Blick Art Catalogue and order a large supply of French Wax (the red one) which is formulated for that specific use. 25lbs, is very very cheap, and poses no risks to use at high temps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, January 31, 2007 5:58 PM To: GMartin@marshallhospital.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Plus Wax is wax is wax. Ren? J. GMartin@marshallhospital.org wrote: Does any know if embedding wax (Paraplast Plus) can be used in making jewelry by the lost wax method. This would not be used paraffin. We had a flood and several bags of paraffin were damaged ... I don't want to use them for processing. A local high school teacher would like to use them in class to make jewelry. Gary Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From GMartin <@t> marshallhospital.org Thu Feb 1 09:41:43 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Thu Feb 1 09:39:40 2007 Subject: [Histonet] Paraplast Message-ID: Thank you very much for all the responses. The decision is to not use the wax. Gary Martin From froyer <@t> bitstream.net Thu Feb 1 09:42:27 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Feb 1 09:43:06 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: <6.2.5.6.2.20070201094459.01c5c398@vet.upenn.edu> References: <20070131225819.72393.qmail@web61224.mail.yahoo.com> <6.2.5.6.2.20070201094459.01c5c398@vet.upenn.edu> Message-ID: <004401c74617$94069540$7701a80a@Ford> Clarification please... In mentioning "DMSO" are we talking about Dimethyl Sulfoxide? ~ Ford Minnesota Medical, Inc. Golden Valley, MN 55427-3601 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, February 01, 2007 8:46 AM To: Terri Braud; Histonet (E-mail) Subject: RE: [Histonet] Paraplast Plus I agree with Terri. Paraplus Plus contains DMSO and is for recommended for reuse. Pam From pmarcum <@t> vet.upenn.edu Thu Feb 1 10:01:45 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Feb 1 10:02:23 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: <004401c74617$94069540$7701a80a@Ford> References: <20070131225819.72393.qmail@web61224.mail.yahoo.com> <6.2.5.6.2.20070201094459.01c5c398@vet.upenn.edu> <004401c74617$94069540$7701a80a@Ford> Message-ID: <6.2.5.6.2.20070201110130.01c8ac98@vet.upenn.edu> Yes, that is DMSO. Pam At 10:42 AM 2/1/2007, Ford Royer wrote: >Clarification please... In mentioning "DMSO" are we talking about Dimethyl >Sulfoxide? > >~ Ford > >Minnesota Medical, Inc. >Golden Valley, MN 55427-3601 >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela >Marcum >Sent: Thursday, February 01, 2007 8:46 AM >To: Terri Braud; Histonet (E-mail) >Subject: RE: [Histonet] Paraplast Plus > >I agree with Terri. Paraplus Plus contains DMSO >and is for recommended for reuse. Pam > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From jqb7 <@t> cdc.gov Thu Feb 1 09:58:54 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Feb 1 10:02:55 2007 Subject: [Histonet] Paraplast Plus References: <20070131225819.72393.qmail@web61224.mail.yahoo.com><6.2.5.6.2.20070201094459.01c5c398@vet.upenn.edu> <004401c74617$94069540$7701a80a@Ford> Message-ID: Yep! But in a very small quantity. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Thursday, February 01, 2007 10:42 AM To: 'Histonet (E-mail)' Subject: RE: [Histonet] Paraplast Plus Clarification please... In mentioning "DMSO" are we talking about Dimethyl Sulfoxide? ~ Ford Minnesota Medical, Inc. Golden Valley, MN 55427-3601 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, February 01, 2007 8:46 AM To: Terri Braud; Histonet (E-mail) Subject: RE: [Histonet] Paraplast Plus I agree with Terri. Paraplus Plus contains DMSO and is for recommended for reuse. Pam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Feb 1 10:17:10 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Feb 1 10:17:16 2007 Subject: [Histonet] ASCP Exam Long opinion Message-ID: Joe You owe me big on this as I'm sure that it will take the flaming away from you. My personal opinion is that what is needed for the entire system is a good enema! First I have a lot of sympathy and admiration for people who prepare and mark examinations, after all I do this a lot and it is a thankless task. However, the concept of having an examination without a practical component to certify individuals as competent is one of the most stupid things I have ever heard (bearing in mind that I am in my late 60s and have worked in labs since 1957 that should give you some idea of how stupid I feel this is.) I also felt that being able to send microscope slides in for evaluation and being able use automatic slide stainers for preparation of such slides comes a close second. >From many comments I am assuming that what is behind this entire movement to dumb down the process is financial. This is the same mentality that is used in education nowadays. The question that is being asked seems to be what can we do with what we have? Put another way, how can we for example expand the work but use the same number of people? The question that should be asked is what resources do we need to get the job done most efficiently? I feel that most jobs can be most efficiently carried out with highly trained and happy individuals. The careers and well being of individuals involved in the process appears in may labs to not be a high priority. I was trained in England and so I feel that I perhaps have a broader view of the training that is carried out in the States and I have seen two retrograde steps. The first was to remove histology from the med lab tech curriculum. The second was to have evaluation of histotechs under the jurisdiction of ASCP. I think that ASCP does a great job in many ways, however this is akin to having the fox in charge of the henhouse. In many ways I feel that this has directly or indirectly contributed to the low salaries for many histotechs. I feel that what is required is a training and an examination system that is on a national level and that will maintain standards of excellence. I am not certain of the same system I trained under in England is still in operation but I felt that it was a system that benefited both employees and employers. If you were employed in any medically associated laboratory it was mandatory for you to have one day and 1 evening of your own time for training at a nationally recognized facility. The employer paid for your day off and the main requirement was that you maintained good grades. This training covered several disciplines e.g. histopathology, hematology and blood banking, histopathology, bacteriology, clinical chemistry etc. Training took three years. At the end of three years you took a written examination over all topics and if you passed this a practical examination. The practical examinations were at local centers. You were in a lab where you were presented with fresh tissue, fluids, and supplies and a list of tasks to accomplish in a morning. You multitasked - the order you carried out these tasks were entirely up to you. In the afternoon you had an oral examination from a panel of three people. If you passed all parts you were recognized as a qualified Med Lab Tech. You could go into any lab in the country and would be guaranteed a salary range and more importantly the laboratory you went to would know that, regardless of the lab you had worked in, that you had a set of uniform skills in the entire area. Everyone benefited from this. If you wished you could carry out advanced training in areas such as histopathology, bacteriology etc. this required a further two years. The net result of all this was that many labs has people at all levels of training who acted as mentors. There were clear cut career paths. I hope that the employers who survived a hear attack at the prospect of implementing such a system see the underlying message. First you need to train people and not just in a limited area. Second that such training is often not available at the lab you are working in and this requires a standardized training and evaluation system. Lastly that a specific career path is established for employees from day one with obligations form both the employer and the employee. While the federal government would totally screw up such a system we do have an NSH that could set standards and allow each state to enforce such standards. Thank y'all who have read these ramblings. I promise you that I am not smoking anything. Barry From jreichensperger <@t> siumed.edu Thu Feb 1 10:21:19 2007 From: jreichensperger <@t> siumed.edu (Joel Reichensperger) Date: Thu Feb 1 10:21:30 2007 Subject: [Histonet] Alizarin Red stain Message-ID: <45C2137F.9010007@siumed.edu> Hi everyone, We are currently trying to stain osteoblasts for calcium using Alizarin Red S at 2%, but recently we have been having problems with the stain starting to gel within 10 minutes of making it and it also is not staining. I am not sure what we are doing wrong. It worked fine until about 3 weeks ago. Nothing has changed. Normally we see nice red areas of calcium and now we have no staining at all. We add 2g of Alizarin red S to 100ml of dH2O and then adjust the pH to 4.1-4.3 using ammonium hydroxide. Is anyone else using this stain and how do you make it? -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute 217-545-7309 (Office) 217-545-1824 (Fax) From froyer <@t> bitstream.net Thu Feb 1 10:22:25 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Feb 1 10:23:00 2007 Subject: [Histonet] Paraplast Plus In-Reply-To: <6.2.5.6.2.20070201110130.01c8ac98@vet.upenn.edu> References: <20070131225819.72393.qmail@web61224.mail.yahoo.com> <6.2.5.6.2.20070201094459.01c5c398@vet.upenn.edu> <004401c74617$94069540$7701a80a@Ford> <6.2.5.6.2.20070201110130.01c8ac98@vet.upenn.edu> Message-ID: <004601c7461d$28d9d970$7701a80a@Ford> That's what I thought, but I wanted to make sure. I personally believe that the "hazards" of DMSO are highly overrated (other than the garlic-breath side affects... eeuuwgh phew). I put DMSO toxicity in the same category of amalgam dental fillings and fluoridation of public water. I believe the jury is still out on DMSO. But... it is true that the first city in the USA that fluoridated their public water system was Royersford, PA in 1859. It is also a matter of public record that NONE of those people are alive today! So caution is the better part of valor here. Move over JOE! I'm headed to join you in the flame resistant bunker!! ;-) ~ Ford Minnesota Medical, Inc. Golden Valley, MN 55427-3601 -----Original Message----- From: Pamela Marcum [mailto:pmarcum@vet.upenn.edu] Sent: Thursday, February 01, 2007 10:02 AM To: Ford Royer; 'Histonet (E-mail)' Subject: RE: [Histonet] Paraplast Plus Yes, that is DMSO. Pam At 10:42 AM 2/1/2007, Ford Royer wrote: >Clarification please... In mentioning "DMSO" are we talking about Dimethyl >Sulfoxide? > >~ Ford From KMH.02 <@t> ex.uchs.org Thu Feb 1 10:29:33 2007 From: KMH.02 <@t> ex.uchs.org (Hopkins, Karen) Date: Thu Feb 1 10:29:47 2007 Subject: [Histonet] RE: Histonet Digest, Vol 39, Issue 1 HT PRACTICAL Message-ID: <640B23A5DC4B234BB065E56F2DB30596028AB438@uchex2ucmc.uchs.org> What does it really prove when a potential histotech can take days and numerous runs to produce one perfect slide for the practical. It does not replicate the real world. I think the amount of job related histology experience will be your indicator and even that is not a given but it is more in line with reality. From jmahoney <@t> alegent.org Thu Feb 1 10:33:12 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Feb 1 10:33:27 2007 Subject: [Histonet] ASCP Exam In-Reply-To: References: <004e01c745a2$eaf016d0$649eae18@yourxhtr8hvc4p> Message-ID: <45C1C1E80200003C00003AB7@gwia.alegent.org> As a Histology manager, I want techs that have it all. A person who is a good microtomist is valuable only to a point and a tech who has all the knowledge and low skill is only half a tech. It is up to the employers to assure competency in all areas, not the ASCP. When you hire a new tech it is always a gamble even with the best references and screening process. I have hired registered HT's who don't know or remember squat and I have hired "unregistered" techs who have it all. We have a probationary period during which I would not hesitate to let someone go if they showed no promise of being competent in all areas. Hire slowly, fire quickly is a good motto. I personally am an advocate of requiring HT(ASCP) certification because it raises the field to a more professional level. I know this would leave some labs in dire straights for techs. I would advocate for a grandfather clause. Maybe it is Friday....... Jan Omaha >>> Jennifer MacDonald 01/31/2007 8:05 PM >>> Joe, I agree with you that skill is very important to be a competent histotech. What I disagree with is that the ASCP practical exam was a fair measure of the competence of the skill of the histotech. Your employees' situation is a classic example. Do you feel that one was more competent than the other? Was one set a little better than the other? I also know of an example where a tech with more than 25 years experience did the practical for an individual and the applicant failed. Time on the bench is also not a good measure of skill. I have graded slides at the ASCP and I have to tell you that it is very difficult to give a failing grade. Others must agree with you before you can deduct "big" points. Also no one person grades a set of slides. A minimum of three graders will see the slides. How close were the scores, if you don't mind me asking? When grading the slides the graders are not looking for slides of diagnostic value, they are looking for perfect. Right or wrong that was the standard that every applicant was held to. As I said, there is no perfect way to test the competence of applicants. In my opinion the practical needed to go. That is not to say that when interviewing job applicants you cannot ask them to prove their microtomy skills. In my experience if an applicant has a strong working knowledge of the theory and some microtomy experience it is easier to train them than it is to train someone with only microtomy skills. It also depends on what you want them to accomplish in the lab. If you strictly need a good microtomist than really good microtomy skills are necessary. If you need someone to be able to troubleshoot staining problems the good microtomist would not be the person for the job. That does not make one a better tech than the other. It all depends on the needs and priorities of the situation. Jennifer "Joe Nocito" 01/31/2007 05:47 PM To "Jennifer MacDonald" , "Larry Woody" cc , , "Chen, Leslie" Subject Re: [Histonet] ASCP Exam It is Friday!!!!! Jennifer, I must respectfully disagree with you. I have two techs that obtained tissue from the same autopsy, processed, embedded, stained and coverslipped said tissue at the same time. I reviewed the slides as did my medical director. The techs were getting mad at me because I kept kicking slides back for one reason or another. One passed the practical, one didn't. I still don't understand how that happened. In essence, who ever graded those slides said that my pathologist and I didn't know what we were doing. Now, on the other hand, all prospective employees that apply at my lab sit down and cut a few blocks to demonstrate that they know how to handle a microtome. Book knowledge is one thing, but you have to agree in the histo lab, skill is very important. Just my 3 cents. Joe "let the flaming begin, again" BS, PA, HT(ASCP)QIHC ----- Original Message ----- From: "Jennifer MacDonald" To: "Larry Woody" Cc: ; ; "Chen, Leslie" Sent: Wednesday, January 31, 2007 7:15 PM Subject: Re: [Histonet] ASCP Exam > Lack of a practical has nothing to do with mandatory certification. The > practical was a component of the HT/HTL (ASCP) certification exam. Passing > of the exam gives the applicant national certification with the ASCP. > Mandating certification may very well be dictated by pathologists, but it > has ABSOLUTELY NOTHING to do with the practical aspect of the HT/HTL exam > being discontinued. > It is interesting to note that no other laboratory science certification > requires a practical. Does that make those certified applicants any less > competent? > Expense was not the only criteria that was used to make the decision to > discontinue the practical. There were many factors. With HIIPA > regulations obtaining the tissue was also becoming difficult for > applicants. Some applicants have access to fully automated labs, while > others don't. Some out there are unethical and don't do any of the work > themselves. What makes it a fair and valid exam. At least with the > "written" portion applicants must show knowledge of the principles and > procedures and the ability to recognize problems and to troubleshoot them. > > Sending the slides to the graders is not an option. The ASCP had a very > sophisticated grading system to ensure anonymity to each applicant and to > ensure that the grading was equitable. Sending out slides to graders > would have compromised a fair system. You also open up the possibility of > breakage and lost slides/blocks. > There is no method of examination that will please everyone. The ASCP, > who had feedback from many people in the histology field, made the > decision that they felt would be fair to MOST applicants. > The ability to cut good sections does not make one a good histotech, just > a good microtomist. Just as the ability to pass the computer portion of > the exam does not make one a good histotech, but the odds are better for a > well rounded employee. One needs to understand and interpret the stains > they are performing, not just load them on the Stainer. > Just my opinion, > Jennifer MacDonald > > > > > Larry Woody > 01/31/2007 02:04 PM > > To > Jennifer MacDonald , "Chen, Leslie" > > cc > "'histonet@lists.utsouthwestern.edu'" , > histonet-bounces@lists.utsouthwestern.edu > Subject > Re: [Histonet] ASCP Exam > > > > > > > Hate to say it but I think Pathologists have a lot to do with why there > isn't mandatory certification for HT and HTL. > > Jennifer MacDonald wrote: > The practical will only apply to those that did not pass prior to 2007. > Anyone that applies for 2007 and beyond does not do the practical. > The instructions state do not download the practical instructions unless > told to do so by the ASCP > > > > > > > "Chen, Leslie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/31/2007 11:42 AM > > To > "'histonet@lists.utsouthwestern.edu'" > cc > > Subject > [Histonet] ASCP Exam > > > > > > > I'm sorry, I'm confused about the conversations regarding the practical > portion of the exam. Are you saying that the practical part is no longer > required for HT or HTL cert for ASCP? According to their website, the > practical is still required. There are practical instructions: > https://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/el > > > igibility/htl.aspx > Thanks. > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. If you are not the intended > recipient, please immediately notify us by return email, and delete this > message from your computer. > ---------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Don't be flakey. Get Yahoo! Mail for Mobile and > always stay connected to friends. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Thu Feb 1 10:40:11 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Feb 1 10:40:23 2007 Subject: [Histonet] RE: Histonet Digest, Vol 39, Issue 1 HT PRACTICAL In-Reply-To: <640B23A5DC4B234BB065E56F2DB30596028AB438@uchex2ucmc.uchs.org> Message-ID: <672418.5543.qm@web53615.mail.yahoo.com> I don't care what kind of exams there are, there will still be people getting in the field who are great at the job and ones who aren't. What we really need are incentives for more talent to enter the field from the start and in today's world that is $$$. There are more people leaving via retirement, vacating, other professions, than there are people coming in and you don't need an exam to figure out why. I have worked with a lot of great techs over the years who have left because of better opportunities and more $$$. "Hopkins, Karen" wrote: What does it really prove when a potential histotech can take days and numerous runs to produce one perfect slide for the practical. It does not replicate the real world. I think the amount of job related histology experience will be your indicator and even that is not a given but it is more in line with reality. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. From dahmed <@t> mdanderson.org Thu Feb 1 10:28:45 2007 From: dahmed <@t> mdanderson.org (dahmed@mdanderson.org) Date: Thu Feb 1 10:43:01 2007 Subject: [Histonet] Congo Red controls Message-ID: Does anyone know of a good supply company for Congo Red controls? Thank you. David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center From ree3 <@t> leicester.ac.uk Thu Feb 1 10:38:59 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Feb 1 10:50:05 2007 Subject: [Histonet] ASCP Exam In-Reply-To: <45C1C1E80200003C00003AB7@gwia.alegent.org> References: <004e01c745a2$eaf016d0$649eae18@yourxhtr8hvc4p> <45C1C1E80200003C00003AB7@gwia.alegent.org> Message-ID: Rubbish in(unqualified staff) rubbish out(crappy sections), it's not rocket science to work that out. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: 01 February 2007 16:33 To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Joe Nocito Cc: histonet@lists.utsouthwestern.edu; Leslie Chen Subject: Re: [Histonet] ASCP Exam As a Histology manager, I want techs that have it all. A person who is a good microtomist is valuable only to a point and a tech who has all the knowledge and low skill is only half a tech. It is up to the employers to assure competency in all areas, not the ASCP. When you hire a new tech it is always a gamble even with the best references and screening process. I have hired registered HT's who don't know or remember squat and I have hired "unregistered" techs who have it all. We have a probationary period during which I would not hesitate to let someone go if they showed no promise of being competent in all areas. Hire slowly, fire quickly is a good motto. I personally am an advocate of requiring HT(ASCP) certification because it raises the field to a more professional level. I know this would leave some labs in dire straights for techs. I would advocate for a grandfather clause. Maybe it is Friday....... Jan Omaha >>> Jennifer MacDonald 01/31/2007 8:05 PM >>> Joe, I agree with you that skill is very important to be a competent histotech. What I disagree with is that the ASCP practical exam was a fair measure of the competence of the skill of the histotech. Your employees' situation is a classic example. Do you feel that one was more competent than the other? Was one set a little better than the other? I also know of an example where a tech with more than 25 years experience did the practical for an individual and the applicant failed. Time on the bench is also not a good measure of skill. I have graded slides at the ASCP and I have to tell you that it is very difficult to give a failing grade. Others must agree with you before you can deduct "big" points. Also no one person grades a set of slides. A minimum of three graders will see the slides. How close were the scores, if you don't mind me asking? When grading the slides the graders are not looking for slides of diagnostic value, they are looking for perfect. Right or wrong that was the standard that every applicant was held to. As I said, there is no perfect way to test the competence of applicants. In my opinion the practical needed to go. That is not to say that when interviewing job applicants you cannot ask them to prove their microtomy skills. In my experience if an applicant has a strong working knowledge of the theory and some microtomy experience it is easier to train them than it is to train someone with only microtomy skills. It also depends on what you want them to accomplish in the lab. If you strictly need a good microtomist than really good microtomy skills are necessary. If you need someone to be able to troubleshoot staining problems the good microtomist would not be the person for the job. That does not make one a better tech than the other. It all depends on the needs and priorities of the situation. Jennifer "Joe Nocito" 01/31/2007 05:47 PM To "Jennifer MacDonald" , "Larry Woody" cc , , "Chen, Leslie" Subject Re: [Histonet] ASCP Exam It is Friday!!!!! Jennifer, I must respectfully disagree with you. I have two techs that obtained tissue from the same autopsy, processed, embedded, stained and coverslipped said tissue at the same time. I reviewed the slides as did my medical director. The techs were getting mad at me because I kept kicking slides back for one reason or another. One passed the practical, one didn't. I still don't understand how that happened. In essence, who ever graded those slides said that my pathologist and I didn't know what we were doing. Now, on the other hand, all prospective employees that apply at my lab sit down and cut a few blocks to demonstrate that they know how to handle a microtome. Book knowledge is one thing, but you have to agree in the histo lab, skill is very important. Just my 3 cents. Joe "let the flaming begin, again" BS, PA, HT(ASCP)QIHC ----- Original Message ----- From: "Jennifer MacDonald" To: "Larry Woody" Cc: ; ; "Chen, Leslie" Sent: Wednesday, January 31, 2007 7:15 PM Subject: Re: [Histonet] ASCP Exam > Lack of a practical has nothing to do with mandatory certification. The > practical was a component of the HT/HTL (ASCP) certification exam. Passing > of the exam gives the applicant national certification with the ASCP. > Mandating certification may very well be dictated by pathologists, but it > has ABSOLUTELY NOTHING to do with the practical aspect of the HT/HTL exam > being discontinued. > It is interesting to note that no other laboratory science certification > requires a practical. Does that make those certified applicants any less > competent? > Expense was not the only criteria that was used to make the decision to > discontinue the practical. There were many factors. With HIIPA > regulations obtaining the tissue was also becoming difficult for > applicants. Some applicants have access to fully automated labs, while > others don't. Some out there are unethical and don't do any of the work > themselves. What makes it a fair and valid exam. At least with the > "written" portion applicants must show knowledge of the principles and > procedures and the ability to recognize problems and to troubleshoot them. > > Sending the slides to the graders is not an option. The ASCP had a very > sophisticated grading system to ensure anonymity to each applicant and to > ensure that the grading was equitable. Sending out slides to graders > would have compromised a fair system. You also open up the possibility of > breakage and lost slides/blocks. > There is no method of examination that will please everyone. The ASCP, > who had feedback from many people in the histology field, made the > decision that they felt would be fair to MOST applicants. > The ability to cut good sections does not make one a good histotech, just > a good microtomist. Just as the ability to pass the computer portion of > the exam does not make one a good histotech, but the odds are better for a > well rounded employee. One needs to understand and interpret the stains > they are performing, not just load them on the Stainer. > Just my opinion, > Jennifer MacDonald > > > > > Larry Woody > 01/31/2007 02:04 PM > > To > Jennifer MacDonald , "Chen, Leslie" > > cc > "'histonet@lists.utsouthwestern.edu'" , > histonet-bounces@lists.utsouthwestern.edu > Subject > Re: [Histonet] ASCP Exam > > > > > > > Hate to say it but I think Pathologists have a lot to do with why there > isn't mandatory certification for HT and HTL. > > Jennifer MacDonald wrote: > The practical will only apply to those that did not pass prior to 2007. > Anyone that applies for 2007 and beyond does not do the practical. > The instructions state do not download the practical instructions unless > told to do so by the ASCP > > > > > > > "Chen, Leslie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/31/2007 11:42 AM > > To > "'histonet@lists.utsouthwestern.edu'" > cc > > Subject > [Histonet] ASCP Exam > > > > > > > I'm sorry, I'm confused about the conversations regarding the practical > portion of the exam. Are you saying that the practical part is no longer > required for HT or HTL cert for ASCP? According to their website, the > practical is still required. There are practical instructions: > https://www.ascp.org/Certification/CertifyingExaminations/cert_procedure s/el > > > igibility/htl.aspx > Thanks. > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. If you are not the intended > recipient, please immediately notify us by return email, and delete this > message from your computer. > ---------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Don't be flakey. Get Yahoo! Mail for Mobile and > always stay connected to friends. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Thu Feb 1 11:11:32 2007 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Thu Feb 1 11:13:52 2007 Subject: [Histonet] RE: DAKO Vendor Message-ID: <20070201.091218.18248.1815579@webmail05.nyc.untd.com> Thanks to all who gave me info on the DAKO Vendor. Marsha From hmyers-bird <@t> rrmc.org Thu Feb 1 11:16:53 2007 From: hmyers-bird <@t> rrmc.org (Heidi Myers-Bird) Date: Thu Feb 1 11:18:23 2007 Subject: [Histonet] RE: Histonet Digest, Vol 39, Issue 1 Message-ID: Hello histo world, is there anyone out there that does either double or triple staining using the Dako autostainer. We would like to start trying it and would like some feedback from others that they be doing the same. thanks, Heidi Bird, Rutland, VT This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From rjbuesa <@t> yahoo.com Thu Feb 1 11:32:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 1 11:32:27 2007 Subject: [Histonet] ASCP Exam Message-ID: <199620.77200.qm@web61211.mail.yahoo.com> Hi fellow "histonetters": Although late into this thread I would like to add "my 2 cents" (or "3 cents" due to inflation as Joe Nocito once said to me): A certification, a credential, a diploma does not bestow competence or ability, not only in histology, but in any profession. There are licensed physicians I would not let treat my cat, or attorneys with full bar credentials that could lead you directly to the gallows. This does not mean that a HT should be technology ignorant and just with great dextereity.There are HT that do beautiful histochemistry procedures but do not have the slightiest idea of the chemistry involved, and this is not only bad, but unacceptable. The HT, as well as any practicing member of any profession, should know the science and technique behind the profession, but the practical ability is not obtained by a test, as a matter of fact, tests and grades are usually poor indicators of personal ability and ulterior success in each profession. A HT or HTL (ASCP) cerification is a wonderful salary bargaining "tool" but does not guarantee quality of work. Quality of work is only obtained through practice, by following standards of performance, with PI programs and close supervision, "seasoned" with a decent salary and deserved commendations and encouragement. While in New York the great violinist Isaak Stern was asked by somebody in the street instructions of how to get to Carnegie Hall, and the violinist's answer was: "Practice, practice, practice". Ren? J. --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From ian.montgomery <@t> bio.gla.ac.uk Thu Feb 1 11:51:39 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Feb 1 11:51:54 2007 Subject: Fw: [Histonet] ASCP Exam Long opinion Message-ID: <009401c74629$a06ab660$4724d182@ibls.gla.ac.uk> Joe, I'm with Barry on this, training and good training is essential. I came through the UK university system and like Barry it was paid day-release and evening classes spread over a number of years. Educationally my path was clear, ONC, HNC, BSc and finally PhD, all paid for by my employer. This was coupled with high quality on-job training in whatever discipline you had chosen. Benefit for me, educated and highly trained. My employer, a hard working and loyal employee of forty years. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Rittman, Barry R" To: Sent: Thursday, February 01, 2007 4:17 PM Subject: [Histonet] ASCP Exam Long opinion Joe You owe me big on this as I'm sure that it will take the flaming away from you. My personal opinion is that what is needed for the entire system is a good enema! First I have a lot of sympathy and admiration for people who prepare and mark examinations, after all I do this a lot and it is a thankless task. However, the concept of having an examination without a practical component to certify individuals as competent is one of the most stupid things I have ever heard (bearing in mind that I am in my late 60s and have worked in labs since 1957 that should give you some idea of how stupid I feel this is.) I also felt that being able to send microscope slides in for evaluation and being able use automatic slide stainers for preparation of such slides comes a close second. >From many comments I am assuming that what is behind this entire movement to dumb down the process is financial. This is the same mentality that is used in education nowadays. The question that is being asked seems to be what can we do with what we have? Put another way, how can we for example expand the work but use the same number of people? The question that should be asked is what resources do we need to get the job done most efficiently? I feel that most jobs can be most efficiently carried out with highly trained and happy individuals. The careers and well being of individuals involved in the process appears in may labs to not be a high priority. I was trained in England and so I feel that I perhaps have a broader view of the training that is carried out in the States and I have seen two retrograde steps. The first was to remove histology from the med lab tech curriculum. The second was to have evaluation of histotechs under the jurisdiction of ASCP. I think that ASCP does a great job in many ways, however this is akin to having the fox in charge of the henhouse. In many ways I feel that this has directly or indirectly contributed to the low salaries for many histotechs. I feel that what is required is a training and an examination system that is on a national level and that will maintain standards of excellence. I am not certain of the same system I trained under in England is still in operation but I felt that it was a system that benefited both employees and employers. If you were employed in any medically associated laboratory it was mandatory for you to have one day and 1 evening of your own time for training at a nationally recognized facility. The employer paid for your day off and the main requirement was that you maintained good grades. This training covered several disciplines e.g. histopathology, hematology and blood banking, histopathology, bacteriology, clinical chemistry etc. Training took three years. At the end of three years you took a written examination over all topics and if you passed this a practical examination. The practical examinations were at local centers. You were in a lab where you were presented with fresh tissue, fluids, and supplies and a list of tasks to accomplish in a morning. You multitasked - the order you carried out these tasks were entirely up to you. In the afternoon you had an oral examination from a panel of three people. If you passed all parts you were recognized as a qualified Med Lab Tech. You could go into any lab in the country and would be guaranteed a salary range and more importantly the laboratory you went to would know that, regardless of the lab you had worked in, that you had a set of uniform skills in the entire area. Everyone benefited from this. If you wished you could carry out advanced training in areas such as histopathology, bacteriology etc. this required a further two years. The net result of all this was that many labs has people at all levels of training who acted as mentors. There were clear cut career paths. I hope that the employers who survived a hear attack at the prospect of implementing such a system see the underlying message. First you need to train people and not just in a limited area. Second that such training is often not available at the lab you are working in and this requires a standardized training and evaluation system. Lastly that a specific career path is established for employees from day one with obligations form both the employer and the employee. While the federal government would totally screw up such a system we do have an NSH that could set standards and allow each state to enforce such standards. Thank y'all who have read these ramblings. I promise you that I am not smoking anything. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Feb 1 12:13:13 2007 From: jnocito <@t> satx.rr.com (jnocito@satx.rr.com) Date: Thu Feb 1 12:13:21 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: References: Message-ID: ok Barry, how does lunch sound? I'll buy lunch on Saturday during the TSH. How's that? No, no, you don't have to thank me. It'll be my honor. ----- Original Message ----- From: "Rittman, Barry R" Date: Thursday, February 1, 2007 10:22 am Subject: [Histonet] ASCP Exam Long opinion To: Histonet@lists.utsouthwestern.edu > Joe > You owe me big on this as I'm sure that it will take the flaming away > from you. > > My personal opinion is that what is needed for the entire system > is a > good enema! > > First I have a lot of sympathy and admiration for people who > prepare and > mark examinations, after all I do this a lot and it is a thankless > task. > However, the concept of having an examination without a practical > component to certify individuals as competent is one of the most > stupidthings I have ever heard (bearing in mind that I am in my > late 60s and > have worked in labs since 1957 that should give you some idea of how > stupid I feel this is.) > I also felt that being able to send microscope slides in for > evaluationand being able use automatic slide stainers for > preparation of such > slides comes a close second. > >From many comments I am assuming that what is behind this entire > movement to dumb down the process is financial. > This is the same mentality that is used in education nowadays. > The question that is being asked seems to be what can we do with > what we > have? Put another way, how can we for example expand the work but use > the same number of people? > The question that should be asked is what resources do we need to get > the job done most efficiently? > I feel that most jobs can be most efficiently carried out with highly > trained and happy individuals. The careers and well being of > individualsinvolved in the process appears in may labs to not be a > high priority. > > I was trained in England and so I feel that I perhaps have a broader > view of the training that is carried out in the States and I have seen > two retrograde steps. > The first was to remove histology from the med lab tech > curriculum. The > second was to have evaluation of histotechs under the jurisdiction of > ASCP. > I think that ASCP does a great job in many ways, however this is > akin to > having the fox in charge of the henhouse. > In many ways I feel that this has directly or indirectly > contributed to > the low salaries for many histotechs. > I feel that what is required is a training and an examination system > that is on a national level and that will maintain standards of > excellence. > I am not certain of the same system I trained under in England is > stillin operation but I felt that it was a system that benefited both > employees and employers. > If you were employed in any medically associated laboratory it was > mandatory for you to have one day and 1 evening of your own time for > training at a nationally recognized facility. > The employer paid for your day off and the main requirement was > that you > maintained good grades. This training covered several disciplines e.g. > histopathology, hematology and blood banking, histopathology, > bacteriology, clinical chemistry etc. Training took three years. > At the > end of three years you took a written examination over all topics > and if > you passed this a practical examination. The practical > examinations were > at local centers. You were in a lab where you were presented with > freshtissue, fluids, and supplies and a list of tasks to > accomplish in a > morning. You multitasked - the order you carried out these tasks were > entirely up to you. > In the afternoon you had an oral examination from a panel of three > people. > > If you passed all parts you were recognized as a qualified Med Lab > Tech.You could go into any lab in the country and would be > guaranteed a > salary range and more importantly the laboratory you went to would > knowthat, regardless of the lab you had worked in, that you had a > set of > uniform skills in the entire area. Everyone benefited from this. > If you wished you could carry out advanced training in areas such as > histopathology, bacteriology etc. this required a further two years. > The net result of all this was that many labs has people at all levels > of training who acted as mentors. There were clear cut career paths. > I hope that the employers who survived a hear attack at the > prospect of > implementing such a system see the underlying message. > First you need to train people and not just in a limited area. > Second that such training is often not available at the lab you are > working in and this requires a standardized training and evaluation > system. > Lastly that a specific career path is established for employees > from day > one with obligations form both the employer and the employee. > While the federal government would totally screw up such a system > we do > have an NSH that could set standards and allow each state to enforce > such standards. > Thank y'all who have read these ramblings. > I promise you that I am not smoking anything. > Barry > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJasper <@t> smdc.org Thu Feb 1 12:17:20 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Thu Feb 1 12:18:14 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A4FB@SCREECH.ntcampus.smdc.org> Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Thursday, February 01, 2007 10:17 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP Exam Long opinion Joe You owe me big on this as I'm sure that it will take the flaming away from you. My personal opinion is that what is needed for the entire system is a good enema! First I have a lot of sympathy and admiration for people who prepare and mark examinations, after all I do this a lot and it is a thankless task. However, the concept of having an examination without a practical component to certify individuals as competent is one of the most stupid things I have ever heard (bearing in mind that I am in my late 60s and have worked in labs since 1957 that should give you some idea of how stupid I feel this is.) I also felt that being able to send microscope slides in for evaluation and being able use automatic slide stainers for preparation of such slides comes a close second. >From many comments I am assuming that what is behind this entire movement to dumb down the process is financial. This is the same mentality that is used in education nowadays. The question that is being asked seems to be what can we do with what we have? Put another way, how can we for example expand the work but use the same number of people? The question that should be asked is what resources do we need to get the job done most efficiently? I feel that most jobs can be most efficiently carried out with highly trained and happy individuals. The careers and well being of individuals involved in the process appears in may labs to not be a high priority. I was trained in England and so I feel that I perhaps have a broader view of the training that is carried out in the States and I have seen two retrograde steps. The first was to remove histology from the med lab tech curriculum. The second was to have evaluation of histotechs under the jurisdiction of ASCP. I think that ASCP does a great job in many ways, however this is akin to having the fox in charge of the henhouse. In many ways I feel that this has directly or indirectly contributed to the low salaries for many histotechs. I feel that what is required is a training and an examination system that is on a national level and that will maintain standards of excellence. I am not certain of the same system I trained under in England is still in operation but I felt that it was a system that benefited both employees and employers. If you were employed in any medically associated laboratory it was mandatory for you to have one day and 1 evening of your own time for training at a nationally recognized facility. The employer paid for your day off and the main requirement was that you maintained good grades. This training covered several disciplines e.g. histopathology, hematology and blood banking, histopathology, bacteriology, clinical chemistry etc. Training took three years. At the end of three years you took a written examination over all topics and if you passed this a practical examination. The practical examinations were at local centers. You were in a lab where you were presented with fresh tissue, fluids, and supplies and a list of tasks to accomplish in a morning. You multitasked - the order you carried out these tasks were entirely up to you. In the afternoon you had an oral examination from a panel of three people. If you passed all parts you were recognized as a qualified Med Lab Tech. You could go into any lab in the country and would be guaranteed a salary range and more importantly the laboratory you went to would know that, regardless of the lab you had worked in, that you had a set of uniform skills in the entire area. Everyone benefited from this. If you wished you could carry out advanced training in areas such as histopathology, bacteriology etc. this required a further two years. The net result of all this was that many labs has people at all levels of training who acted as mentors. There were clear cut career paths. I hope that the employers who survived a hear attack at the prospect of implementing such a system see the underlying message. First you need to train people and not just in a limited area. Second that such training is often not available at the lab you are working in and this requires a standardized training and evaluation system. Lastly that a specific career path is established for employees from day one with obligations form both the employer and the employee. While the federal government would totally screw up such a system we do have an NSH that could set standards and allow each state to enforce such standards. Thank y'all who have read these ramblings. I promise you that I am not smoking anything. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From pathrm35 <@t> charter.net Thu Feb 1 12:35:32 2007 From: pathrm35 <@t> charter.net (pathrm35@charter.net) Date: Thu Feb 1 12:35:42 2007 Subject: [Histonet] RE: Histonet Digest, Vol 39, Issue 1 HT PRACTICAL Message-ID: <1121758254.1170354932773.JavaMail.root@fepweb02> In theory it would seem logical that a properly educated tech (both academics and technical) would be a well rounded potential employee. I think the majority are. I have seen some OJT techs who are more technically competent than some college grads. However, they do lack the theory behind what they are doing and do have difficulty troubleshooting problems. I have also seen college grads who are very sound academically but not technically. The bottom line is that if you have a good tech who is sound academically and technically, hold on to him/her because they are worth their weight in gold. I have seen many poor techs get paid very well for poor work just because of the supply and demand situation we are in. Just my two cents. Ron Martin --- Larry Woody wrote: > I don't care what kind of exams there are, there will still be people getting in the field who are great at the job and ones who aren't. What we really need are incentives for more talent to enter the field from the start and in today's world that is $$$. There are more people leaving via retirement, vacating, other professions, than there are people coming in and you don't need an exam to figure out why. I have worked with a lot of great techs over the years who have left because of better opportunities and more $$$. > > "Hopkins, Karen" wrote: What does it really prove when a potential histotech can take days and > numerous runs to produce one perfect slide for the practical. It does > not replicate the real world. I think the amount of job related > histology experience will be your indicator and even that is not a given > but it is more in line with reality. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Feb 1 12:37:12 2007 From: jnocito <@t> satx.rr.com (jnocito@satx.rr.com) Date: Thu Feb 1 12:37:25 2007 Subject: Fw: [Histonet] ASCP Exam Long opinion In-Reply-To: <009401c74629$a06ab660$4724d182@ibls.gla.ac.uk> References: <009401c74629$a06ab660$4724d182@ibls.gla.ac.uk> Message-ID: Ian, I envy people like you and Barry. Getting funds to attend a state and/or national meeting is like cutting undecalified femur. However, I do get all the coffee I can drink Joe ----- Original Message ----- From: Ian Montgomery Date: Thursday, February 1, 2007 11:54 am Subject: Fw: [Histonet] ASCP Exam Long opinion To: Histonet > Joe, > I'm with Barry on this, training and good training is > essential. I > came through the UK university system and like Barry it was paid > day-release > and evening classes spread over a number of years. Educationally > my path was > clear, ONC, HNC, BSc and finally PhD, all paid for by my employer. > This was > coupled with high quality on-job training in whatever discipline > you had > chosen. Benefit for me, educated and highly trained. My employer, > a hard > working and loyal employee of forty years. > Ian. > > > Dr. Ian Montgomery, > Histotechnology, > IBLS Support Services, > Thomson Building, > University of Glasgow, > Tel:01413398855 > Extn: 8511. > ----- Original Message ----- > From: "Rittman, Barry R" > To: > Sent: Thursday, February 01, 2007 4:17 PM > Subject: [Histonet] ASCP Exam Long opinion > > > Joe > You owe me big on this as I'm sure that it will take the flaming away > from you. > > My personal opinion is that what is needed for the entire system > is a > good enema! > > First I have a lot of sympathy and admiration for people who > prepare and > mark examinations, after all I do this a lot and it is a thankless > task. > However, the concept of having an examination without a practical > component to certify individuals as competent is one of the most > stupidthings I have ever heard (bearing in mind that I am in my > late 60s and > have worked in labs since 1957 that should give you some idea of how > stupid I feel this is.) > I also felt that being able to send microscope slides in for > evaluationand being able use automatic slide stainers for > preparation of such > slides comes a close second. > >From many comments I am assuming that what is behind this entire > movement to dumb down the process is financial. > This is the same mentality that is used in education nowadays. > The question that is being asked seems to be what can we do with > what we > have? Put another way, how can we for example expand the work but use > the same number of people? > The question that should be asked is what resources do we need to get > the job done most efficiently? > I feel that most jobs can be most efficiently carried out with highly > trained and happy individuals. The careers and well being of > individualsinvolved in the process appears in may labs to not be a > high priority. > > I was trained in England and so I feel that I perhaps have a broader > view of the training that is carried out in the States and I have seen > two retrograde steps. > The first was to remove histology from the med lab tech > curriculum. The > second was to have evaluation of histotechs under the jurisdiction of > ASCP. > I think that ASCP does a great job in many ways, however this is > akin to > having the fox in charge of the henhouse. > In many ways I feel that this has directly or indirectly > contributed to > the low salaries for many histotechs. > I feel that what is required is a training and an examination system > that is on a national level and that will maintain standards of > excellence. > I am not certain of the same system I trained under in England is > stillin operation but I felt that it was a system that benefited both > employees and employers. > If you were employed in any medically associated laboratory it was > mandatory for you to have one day and 1 evening of your own time for > training at a nationally recognized facility. > The employer paid for your day off and the main requirement was > that you > maintained good grades. This training covered several disciplines e.g. > histopathology, hematology and blood banking, histopathology, > bacteriology, clinical chemistry etc. Training took three years. > At the > end of three years you took a written examination over all topics > and if > you passed this a practical examination. The practical > examinations were > at local centers. You were in a lab where you were presented with > freshtissue, fluids, and supplies and a list of tasks to > accomplish in a > morning. You multitasked - the order you carried out these tasks were > entirely up to you. > In the afternoon you had an oral examination from a panel of three > people. > > If you passed all parts you were recognized as a qualified Med Lab > Tech.You could go into any lab in the country and would be > guaranteed a > salary range and more importantly the laboratory you went to would > knowthat, regardless of the lab you had worked in, that you had a > set of > uniform skills in the entire area. Everyone benefited from this. > If you wished you could carry out advanced training in areas such as > histopathology, bacteriology etc. this required a further two years. > The net result of all this was that many labs has people at all levels > of training who acted as mentors. There were clear cut career paths. > I hope that the employers who survived a hear attack at the > prospect of > implementing such a system see the underlying message. > First you need to train people and not just in a limited area. > Second that such training is often not available at the lab you are > working in and this requires a standardized training and evaluation > system. > Lastly that a specific career path is established for employees > from day > one with obligations form both the employer and the employee. > While the federal government would totally screw up such a system > we do > have an NSH that could set standards and allow each state to enforce > such standards. > Thank y'all who have read these ramblings. > I promise you that I am not smoking anything. > Barry > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Thu Feb 1 12:43:09 2007 From: jnocito <@t> satx.rr.com (jnocito@satx.rr.com) Date: Thu Feb 1 12:43:17 2007 Subject: [Histonet] ASCP Exam In-Reply-To: <199620.77200.qm@web61211.mail.yahoo.com> References: <199620.77200.qm@web61211.mail.yahoo.com> Message-ID: Rene, once again, I have to agree with you. There are some doctors that I have come across that I wouldn't let them take my temperature. I wonder how they made it through med school and a residency. Good one on the violinist. Joe "keeping my foot in my mouth" Nocito (I really need to watch where I'm stepping) ----- Original Message ----- From: Rene J Buesa Date: Thursday, February 1, 2007 11:36 am Subject: [Histonet] ASCP Exam To: histonet@lists.utsouthwestern.edu > Hi fellow "histonetters": > Although late into this thread I would like to add "my 2 cents" > (or "3 cents" due to inflation as Joe Nocito once said to me): > A certification, a credential, a diploma does not bestow > competence or ability, not only in histology, but in any profession. > There are licensed physicians I would not let treat my cat, or > attorneys with full bar credentials that could lead you directly > to the gallows. > This does not mean that a HT should be technology ignorant and > just with great dextereity.There are HT that do beautiful > histochemistry procedures but do not have the slightiest idea of > the chemistry involved, and this is not only bad, but > unacceptable. > The HT, as well as any practicing member of any profession, > should know the science and technique behind the profession, but > the practical ability is not obtained by a test, as a matter of > fact, tests and grades are usually poor indicators of personal > ability and ulterior success in each profession. > A HT or HTL (ASCP) cerification is a wonderful salary bargaining > "tool" but does not guarantee quality of work. Quality of work is > only obtained through practice, by following standards of > performance, with PI programs and close supervision, "seasoned" > with a decent salary and deserved commendations and encouragement. > > While in New York the great violinist Isaak Stern was asked by > somebody in the street instructions of how to get to Carnegie > Hall, and the violinist's answer was: "Practice, practice, practice". > Ren? J. > > > > > --------------------------------- > Don't be flakey. Get Yahoo! Mail for Mobile and > always stay connected to friends. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ploykasek <@t> phenopath.com Thu Feb 1 13:20:59 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Feb 1 13:21:26 2007 Subject: [Histonet] EBER Message-ID: I have an inquiry for anyone doing EBV in-situ hybridization. What type of probe are you using? How is your probe labeled? Are you using a kit & if so, what kit? We are looking to optimize our sensitivity. Currently we are using a digoxigen labeled EBV probe that we have made for us, detection is via an anti-dig link and then Envision+-HRP, followed by DAB. Thanks for the info. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mauger <@t> email.chop.edu Thu Feb 1 13:58:22 2007 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Thu Feb 1 13:59:09 2007 Subject: [Histonet] EBER Message-ID: Patti, We have been using Dako's PNA ISH kit and their Eber PNA probe with great success for the last 2 years.It is very easy to do and reproducible. Jo Mauger From GDawson <@t> dynacaremilwaukee.com Thu Feb 1 14:08:24 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Feb 1 14:08:40 2007 Subject: [Histonet] EBER Message-ID: I use this one too & I've been very happy with it. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joanne Mauger Sent: Thursday, February 01, 2007 1:58 PM To: histonet@pathology.swmed.edu; ploykasek@phenopath.com Subject: Re: [Histonet] EBER Patti, We have been using Dako's PNA ISH kit and their Eber PNA probe with great success for the last 2 years.It is very easy to do and reproducible. Jo Mauger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwatson <@t> gnf.org Thu Feb 1 14:10:29 2007 From: jwatson <@t> gnf.org (James Watson) Date: Thu Feb 1 14:10:43 2007 Subject: [Histonet] Goat negative control Message-ID: I recently tried to order Goat supersensitive negative control from Biogenex and they have discontinued it. I have started using normal goat serum as my negative control, is this acceptable? If not do you have a source for it. Thank you. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org From shive003 <@t> umn.edu Thu Feb 1 14:30:53 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Feb 1 14:31:05 2007 Subject: [Histonet] Goat negative control References: Message-ID: <00a601c7463f$df24bfc0$a1065486@auxs.umn.edu> James, I use normal goat serum as my negative control serum (when using detection kits that have the link Ab made in goat). Just be sure it's diluted out to the same "IgG concentration" as your primary antiserum working solution. Jan Shivers UMN Vet Diag Lab St. Paul, MN ----- Original Message ----- From: "James Watson" To: Sent: Thursday, February 01, 2007 2:10 PM Subject: [Histonet] Goat negative control I recently tried to order Goat supersensitive negative control from Biogenex and they have discontinued it. I have started using normal goat serum as my negative control, is this acceptable? If not do you have a source for it. Thank you. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Thu Feb 1 14:39:26 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu Feb 1 14:40:23 2007 Subject: [Histonet] EBER Message-ID: Hello Patti, I use Dako's PNA ISH Detection kit Cat# K5201 along with their EBV EBER PNA probe Cat# Y5200 Their protocol is very easy to use. It is a one day run. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Patti Loykasek 02/01/07 2:20 PM >>> I have an inquiry for anyone doing EBV in-situ hybridization. What type of probe are you using? How is your probe labeled? Are you using a kit & if so, what kit? We are looking to optimize our sensitivity. Currently we are using a digoxigen labeled EBV probe that we have made for us, detection is via an anti-dig link and then Envision+-HRP, followed by DAB. Thanks for the info. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bamoe <@t> gundluth.org Thu Feb 1 15:41:50 2007 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Thu Feb 1 15:43:01 2007 Subject: [Histonet] ER control tissue Message-ID: Hello all - Does anyone know of a source to order ER controls for IHC -- other than Pantomics in California? Ideally I'm looking for an array that would contain a strong (3+), moderate (2+), and low/negative (1+) representation. Thanks for any help! Barb Moe Gundersen Lutheran Medical Center La Crosse WI From rjbuesa <@t> yahoo.com Thu Feb 1 16:07:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 1 16:08:03 2007 Subject: [Histonet] Goat negative control In-Reply-To: Message-ID: <352832.44170.qm@web61215.mail.yahoo.com> I used one from DAKO that is multiple (mouse, rabbit and goat) that works very well. Ren? J. James Watson wrote: I recently tried to order Goat supersensitive negative control from Biogenex and they have discontinued it. I have started using normal goat serum as my negative control, is this acceptable? If not do you have a source for it. Thank you. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From histotech <@t> charter.net Thu Feb 1 16:50:42 2007 From: histotech <@t> charter.net (histotech@charter.net) Date: Thu Feb 1 16:50:52 2007 Subject: [Histonet] Frozen Section Stain Message-ID: <904645651.1170370242637.JavaMail.root@fepweb12> We are presently using an auto linear stainer for our frozen sections but would like to change due to the evaporation of the reagents. Would you please share your methods and opinions on other staining procedures. Thanks, DDietz Morristown-Hamblen Histology From RSRICHMOND <@t> aol.com Thu Feb 1 18:29:15 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Feb 1 18:29:33 2007 Subject: [Histonet] Re: Congo Red controls Message-ID: David Ahmed at MDA asks, as so many have, about congo red controls. Human amyloidosis isn't very common, and tissue often isn't very good. It's fairly easy to induce amyloidosis in experimental animals (such as mice). Does anybody use such material for clinical controls? I would think it scientifically acceptable to do so. Bob Richmond Samurai Pathologist Knoxville TN From Luis.Chiriboga <@t> med.nyu.edu Thu Feb 1 12:41:41 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Thu Feb 1 19:13:27 2007 Subject: [Histonet] ASCP Exam.....Tangent In-Reply-To: <199620.77200.qm@web61211.mail.yahoo.com> Message-ID: Hi Everyone I would like to pass along a reference to an editorial piece written by Kevin Roth, Editor-in-Chief of the Journal of Histochemistry and Cytochemistry [V54(10) 1073-74 2006] entitled "A Beautiful Science". I think that most of us will agree with its message. Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, February 01, 2007 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP Exam Hi fellow "histonetters": Although late into this thread I would like to add "my 2 cents" (or "3 cents" due to inflation as Joe Nocito once said to me): A certification, a credential, a diploma does not bestow competence or ability, not only in histology, but in any profession. There are licensed physicians I would not let treat my cat, or attorneys with full bar credentials that could lead you directly to the gallows. This does not mean that a HT should be technology ignorant and just with great dextereity.There are HT that do beautiful histochemistry procedures but do not have the slightiest idea of the chemistry involved, and this is not only bad, but unacceptable. The HT, as well as any practicing member of any profession, should know the science and technique behind the profession, but the practical ability is not obtained by a test, as a matter of fact, tests and grades are usually poor indicators of personal ability and ulterior success in each profession. A HT or HTL (ASCP) cerification is a wonderful salary bargaining "tool" but does not guarantee quality of work. Quality of work is only obtained through practice, by following standards of performance, with PI programs and close supervision, "seasoned" with a decent salary and deserved commendations and encouragement. While in New York the great violinist Isaak Stern was asked by somebody in the street instructions of how to get to Carnegie Hall, and the violinist's answer was: "Practice, practice, practice". Ren? J. --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Fri Feb 2 05:43:08 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Feb 2 05:43:22 2007 Subject: [Histonet] Fw: Reading Leica LIF files - general question on databasing Message-ID: <007f01c746bf$4faa30c0$4724d182@ibls.gla.ac.uk> Rosemary, I'm another Catalyser user and have been for many years. It's flexible, friendly and easy to use. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Sebastian Frische" To: Sent: Friday, February 02, 2007 10:50 AM Subject: Re: Reading Leica LIF files - general question on databasing Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Rosemary You might consider looking at "Catalyzer" from Axiope (www.axiope.com). It is a very versatile cataloging system, and can be downloaded for free for use on a single computer, if you want to try it, but the full version runs with a server. I'm currently implementing Catalyzer in my own work, and I find it very easy to use and extremely flexible. It comes with Leica-import filters, although I haven't tried those yet. (I have no commercial interest) best wishes Sebastian >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Dear all, > >Has anyone solved the problem of a user-friendly image or other type of >database system that can incorporate attached spreadsheets, text documents, >manuscripts, pdfs, etc. in a useful way? I work in a government lab in >which we are supposed to document rigorously everything we do in a standard >A4 lab book, which is eventually transduced into an electronic record which, >in theory, is kept forever. This record-keeping system is called TRIM, and >I know nothing about it except that the archivist rolled her eyes when I >suggested that they incorporate some or all of the digital image data people >generate these days. > >The system was started before the flood of digital images plus image >analyses, etc. began. I'd like at least our microscopy lab to switch to a >fully electronic system - no hard copy lab book. Anyone know of a good >system that molecular biologists and agronomists and everyone in between >would actually use? > >Seems like the main requirement of any system is that people will use it.... > >thanks for any ideas, >cheers, >Rosemary > > > >Dr Rosemary White rosemary.white@csiro.au >CSIRO Plant Industry ph. 61 (0)2-6246 5475 >GPO Box 1600 fax. 61 (0)2-6246 5334 >Canberra, ACT 2601 >Australia -- Sebastian Frische, PhD Assistant Professor The Water and Salt Research Center Institute of Anatomy University of Aarhus Universitetsparken Bygn. 1234 8000 ?rhus C Denmark tel +45 89423025 fax + 45 86198664 From Anna.Inman <@t> stmarygj.org Fri Feb 2 06:42:48 2007 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Fri Feb 2 06:43:07 2007 Subject: [Histonet] Sticky Paraffin Message-ID: <2925AE271EAAD440AF48FCCEB8002D090372F512@smgmail01.smgj.sclhs.net> Happy Friday Histonetters! Recently, our techs have been encountering what is termed here as "sticky paraffin." It is sticking more than usual to the embedder and more importantly to the cutters and creating difficulties at the microtome. We are also encountering much more scratchy tissue that will not ribbon appropriately and I am not sure if the problems are related (maybe a processing issue or entirely separate issues??). It is not everyday but seems to be increasingly worse. We are using Richard Allen Type 9 media. The question has been posed ...should we be using a paraffin with less polymers? Could the sticky paraffin be a result of the weather (air too dry?) Any thoughts? Thank you all for your help! Anna Inman, HT(ASCP) Clinical Resource Specialist SMH Pathology Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tracy.bergeron <@t> crl.com Fri Feb 2 07:53:30 2007 From: tracy.bergeron <@t> crl.com (tracy.bergeron@crl.com) Date: Fri Feb 2 07:54:20 2007 Subject: [Histonet] HT/HTL practical exam - different opinion In-Reply-To: <672418.5543.qm@web53615.mail.yahoo.com> Message-ID: I learned histology as a 1/2 semester independent study while I was in college, back in the early 90's. I figured I would learn it to use as a back up career. Little did I know it would end up as a career. But 99.9% of my experience was OJT, and all of my OJT was with animal tissue. I have always worked in veterinary and research fields of histology. (pays better than clinical - at least in New England anyway) This experience leaves one at a definitely disadvantage when it comes to certification. As far as I know the ACVP has no interest for what ever reason, in offering it's own histology certification programs. So those of us with no human tissue experience who want to get certified are stuck, figuratively speaking, taking the ASCP cert. exams. I am not saying this is a bad thing, but when it comes to taking the practical examination, it can be very difficult to get tissues that fit the size requirements when all the tissue you have access to is from rodents, and occasionally something a little larger. I was lucky enough to have some contacts and was able to get the human tissue needed for both of my practicals, but I have heard of others who have not had such an easy time. By eliminating the practical, in some respects it partially levels the playing field between techs trained in the veterinary and research communities and those trained in a clinical setting. All of the information for the computer examination can be found in text books, and the internet. There is still a lot of information on both exams (more in the HTL than HT), that puts the clinical trained tech at an advantage to the veterinary/research tech. There were a lot of things I had to learn for both exams that I will probably never have to deal with, worry about, or even know, as I have no interest in entering a clinical environment. Now I am in no way complaining about the exams, leaning towards one area above the other because the exams are offered by the ASCP a clinical pathology organization. But at least the veterinary/research folks no longer have to scrounge for tissues to fit the size requirements which are based on human tissue. Figured I would send out a different opinion on the practical. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 From ree3 <@t> leicester.ac.uk Fri Feb 2 08:08:33 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Feb 2 08:08:49 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A4FB@SCREECH.ntcampus.smdc.org> References: <1A9F2A6C5762524799A816F1F09744CF0143A4FB@SCREECH.ntcampus.smdc.org> Message-ID: Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Thursday, February 01, 2007 10:17 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP Exam Long opinion Joe You owe me big on this as I'm sure that it will take the flaming away from you. My personal opinion is that what is needed for the entire system is a good enema! First I have a lot of sympathy and admiration for people who prepare and mark examinations, after all I do this a lot and it is a thankless task. However, the concept of having an examination without a practical component to certify individuals as competent is one of the most stupid things I have ever heard (bearing in mind that I am in my late 60s and have worked in labs since 1957 that should give you some idea of how stupid I feel this is.) I also felt that being able to send microscope slides in for evaluation and being able use automatic slide stainers for preparation of such slides comes a close second. >From many comments I am assuming that what is behind this entire movement to dumb down the process is financial. This is the same mentality that is used in education nowadays. The question that is being asked seems to be what can we do with what we have? Put another way, how can we for example expand the work but use the same number of people? The question that should be asked is what resources do we need to get the job done most efficiently? I feel that most jobs can be most efficiently carried out with highly trained and happy individuals. The careers and well being of individuals involved in the process appears in may labs to not be a high priority. I was trained in England and so I feel that I perhaps have a broader view of the training that is carried out in the States and I have seen two retrograde steps. The first was to remove histology from the med lab tech curriculum. The second was to have evaluation of histotechs under the jurisdiction of ASCP. I think that ASCP does a great job in many ways, however this is akin to having the fox in charge of the henhouse. In many ways I feel that this has directly or indirectly contributed to the low salaries for many histotechs. I feel that what is required is a training and an examination system that is on a national level and that will maintain standards of excellence. I am not certain of the same system I trained under in England is still in operation but I felt that it was a system that benefited both employees and employers. If you were employed in any medically associated laboratory it was mandatory for you to have one day and 1 evening of your own time for training at a nationally recognized facility. The employer paid for your day off and the main requirement was that you maintained good grades. This training covered several disciplines e.g. histopathology, hematology and blood banking, histopathology, bacteriology, clinical chemistry etc. Training took three years. At the end of three years you took a written examination over all topics and if you passed this a practical examination. The practical examinations were at local centers. You were in a lab where you were presented with fresh tissue, fluids, and supplies and a list of tasks to accomplish in a morning. You multitasked - the order you carried out these tasks were entirely up to you. In the afternoon you had an oral examination from a panel of three people. If you passed all parts you were recognized as a qualified Med Lab Tech. You could go into any lab in the country and would be guaranteed a salary range and more importantly the laboratory you went to would know that, regardless of the lab you had worked in, that you had a set of uniform skills in the entire area. Everyone benefited from this. If you wished you could carry out advanced training in areas such as histopathology, bacteriology etc. this required a further two years. The net result of all this was that many labs has people at all levels of training who acted as mentors. There were clear cut career paths. I hope that the employers who survived a hear attack at the prospect of implementing such a system see the underlying message. First you need to train people and not just in a limited area. Second that such training is often not available at the lab you are working in and this requires a standardized training and evaluation system. Lastly that a specific career path is established for employees from day one with obligations form both the employer and the employee. While the federal government would totally screw up such a system we do have an NSH that could set standards and allow each state to enforce such standards. Thank y'all who have read these ramblings. I promise you that I am not smoking anything. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Feb 2 08:53:57 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Feb 2 08:54:16 2007 Subject: [Histonet] Re: ASCP Exam Long opinion Message-ID: Comments on this topic have been thoughtful, interesting, and informative! The American Society for Clinical Pathology (ASCP, it was the American Society of Clinical Pathologists when I joined it in 1966, and the change of name tells you a lot) is not I think responsible for the lamentably low pay histotechnologists receive. I do think that pathologists need to be much more involved in this issue. Most pathologists know little about what goes on the the histology laboratory, and this problem needs to be addressed in residency programs. So we have the common situation in pathology laboratories - the histotechnologist never looks at a slide, and the pathologist never contributes anything but abuse. I've helped enough candidates find tissue for the practical exam (no, we'll have to get tissue from three or four uteri before we get just the right one - no, I can't get you a lumbar spinal cord from my next autopsy because I haven't got the tools and am not allowed to get them) - and I was surprised to see the practical exam eliminated. I think that there could have been some way to get the slides looked at without calling a convention. I'd have been happy to participate in the judging, but I'm not in anybody's inner circle. I wonder if it's time for some organization besides ASCP to set up an alternative path to certification, to counter some of the problems developing in the ASCP certification. As Peggy Wenk points out, formal training programs are badly needed - 25 of them for the whole USA is a pathetic number. I'd love to work with a community college on such a program. Bob Richmond Samurai Pathologist Knoxville TN From Barry.R.Rittman <@t> uth.tmc.edu Fri Feb 2 08:59:07 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Feb 2 08:59:18 2007 Subject: [Histonet] ASCP Exam Long opinion Message-ID: Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN From ree3 <@t> leicester.ac.uk Fri Feb 2 09:24:05 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Feb 2 09:24:41 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: References: Message-ID: Or blue and pink; if the day ever comes when the individual manning the staining machine 'phones up the service engineer complaining "that the blue stuff is not appearing on the small panes of glass with slices on, and what do I do about it??" is the day I hope never to hear about!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: 02 February 2007 14:59 To: Edwards, R.E.; Jasper, Thomas G. Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 2 09:34:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 2 09:35:01 2007 Subject: [Histonet] Sticky Paraffin In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D090372F512@smgmail01.smgj.sclhs.net> Message-ID: <728328.65035.qm@web61219.mail.yahoo.com> Usually paraffin of low melting point is appreciated as sticky. It could be that you have received a batch of lower than usual melting point, or that your paraffin is being contaminated with the antemedium. Also higher than usual temperatures in the sectioning area causes the paraffin to appear sticky. Ren? J. "Inman, Anna" wrote: Happy Friday Histonetters! Recently, our techs have been encountering what is termed here as "sticky paraffin." It is sticking more than usual to the embedder and more importantly to the cutters and creating difficulties at the microtome. We are also encountering much more scratchy tissue that will not ribbon appropriately and I am not sure if the problems are related (maybe a processing issue or entirely separate issues??). It is not everyday but seems to be increasingly worse. We are using Richard Allen Type 9 media. The question has been posed ...should we be using a paraffin with less polymers? Could the sticky paraffin be a result of the weather (air too dry?) Any thoughts? Thank you all for your help! Anna Inman, HT(ASCP) Clinical Resource Specialist SMH Pathology Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From JWEEMS <@t> sjha.org Fri Feb 2 09:34:37 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 2 09:35:09 2007 Subject: [Histonet] HT/HTL practical exam - different opinion Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D324B@sjhaexc02.sjha.org> I feel a need to add my 2 cents... We have whined for years about not being considered as professional as Med Techs... Not having to do a practical is a step closer to being treated the same. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From slappycraw <@t> yahoo.com Fri Feb 2 09:43:21 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Feb 2 09:43:33 2007 Subject: [Histonet] Re: ASCP Exam Long opinion In-Reply-To: Message-ID: <493870.55088.qm@web53614.mail.yahoo.com> The histotechnologist never looks at a slide and most Pathologists know little about what goes on in the histology lab. I guess if I'm looking at a morpheaform bcc stained with an H&E I won't know what I'm looking at and the pathologist that I'm looking at the slide with will have no idea where the slide came from. If it happens to need IHC well forget about it! There are people in the field who don't care to learn what they are doing and that along with no mandatory requirements has kept the compensation low. There are plenty of Pathologists out there willing to look at slides with techs and teach them what's going on and vice versa, as well as plenty of Pathologists willing to hire anybody to cut slides on the cheap. Find out how many Pathologists would be in favor of mandatory requirements for Histotechs and you have your answer. RSRICHMOND@aol.com wrote: Comments on this topic have been thoughtful, interesting, and informative! The American Society for Clinical Pathology (ASCP, it was the American Society of Clinical Pathologists when I joined it in 1966, and the change of name tells you a lot) is not I think responsible for the lamentably low pay histotechnologists receive. I do think that pathologists need to be much more involved in this issue. Most pathologists know little about what goes on the the histology laboratory, and this problem needs to be addressed in residency programs. So we have the common situation in pathology laboratories - the histotechnologist never looks at a slide, and the pathologist never contributes anything but abuse. I've helped enough candidates find tissue for the practical exam (no, we'll have to get tissue from three or four uteri before we get just the right one - no, I can't get you a lumbar spinal cord from my next autopsy because I haven't got the tools and am not allowed to get them) - and I was surprised to see the practical exam eliminated. I think that there could have been some way to get the slides looked at without calling a convention. I'd have been happy to participate in the judging, but I'm not in anybody's inner circle. I wonder if it's time for some organization besides ASCP to set up an alternative path to certification, to counter some of the problems developing in the ASCP certification. As Peggy Wenk points out, formal training programs are badly needed - 25 of them for the whole USA is a pathetic number. I'd love to work with a community college on such a program. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From godsgalnow <@t> aol.com Fri Feb 2 10:13:51 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Feb 2 10:14:07 2007 Subject: [Histonet] anyone still doing cytospins Message-ID: <8C914FC434BAEB5-18EC-10AC@WEBMAIL-RA06.sysops.aol.com> We have switched to the thinprep and still have about 1200 Physician collection kits that we send to out clients that have the saccamanno. We cannot use it. These kits are worth about $2000. If anyone is interested in them, I will sell them to you for $1000 and I will pay to ship them to you. Please let me know ASAP. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, FLorida 813-549-1050 ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From dellav <@t> musc.edu Fri Feb 2 10:19:35 2007 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Feb 2 10:20:08 2007 Subject: [Histonet] pathology list Message-ID: greetings everyone, years ago I used to subscribe to the Patho-L list. then went through a period when I couldn't keep up with both lists and unsubscribed. can anyone tell me if this list still exists and how to subscribe? thanks Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 From rosa_113 <@t> hotmail.com Fri Feb 2 10:34:11 2007 From: rosa_113 <@t> hotmail.com (Rosa Bryant) Date: Fri Feb 2 10:34:23 2007 Subject: [Histonet] A question.. Message-ID: What is the science behind soaking the slides in buffer before IHC procedures? I can't find much of a reference about this, what are the advantages to this step instead of putting the slides back into water? Thanks Rosa Fields _________________________________________________________________ Get the new Windows Live Messenger! http://get.live.com/messenger/overview From GDawson <@t> dynacaremilwaukee.com Fri Feb 2 10:43:45 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Feb 2 10:44:01 2007 Subject: [Histonet] A question.. Message-ID: Rosa, One of the main things is that the detergent in buffer breaks down surface tension allowing subsequent reagent to spread more evenly over the whole slide. It isn't necessary to "soak" them for an extended period of time but I've noticed that, with water only, reagent tends to bead up which can lead to some of the tissue on the slide receiving inadequate treatment. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rosa Bryant Sent: Friday, February 02, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question.. What is the science behind soaking the slides in buffer before IHC procedures? I can't find much of a reference about this, what are the advantages to this step instead of putting the slides back into water? Thanks Rosa Fields _________________________________________________________________ Get the new Windows Live Messenger! http://get.live.com/messenger/overview_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KirkyDog2 <@t> aol.com Fri Feb 2 12:01:02 2007 From: KirkyDog2 <@t> aol.com (KirkyDog2@aol.com) Date: Fri Feb 2 12:01:35 2007 Subject: [Histonet] Histogel and microwave processing Message-ID: Hello everyone, I need help if anyone is willing. We currently process our FNA cell blocks in "Histogel" using an overnight cycle, our cytopathologist would like his results faster. Does anyone know if histogel is compatible with microwave processing techniques, and if the cellular morphology is compromised in any way with such a method? Thank you in advance for your input. T. Kirk From tkngflght <@t> yahoo.com Fri Feb 2 10:24:24 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri Feb 2 12:11:16 2007 Subject: [Histonet] Losing our art form--manual vs. automated In-Reply-To: References: Message-ID: <003d01c746e6$9abe3e90$6401a8c0@FSDESKTOP> Did you all hear the story (true) about the group of newly certified techs and the cover slips? One day the block load was larger than usual and, of course, the coverslipper broke. The newly certified techs were standing around, hands in pockets, because they couldn't turn out the slides. When the old-school manager came in and asked what the holdup was, they responded "We can't turn anything out--they aren't coverslipped." Flabbergasted, the manager donned gloves, sat down and showed them how to apply coverslips the old fashioned way. ________________ I interview 5 or 6 techs a day. I'm finding many have never had the experience the fine art of the hand-stained H&E. I know these machines save time, increase production, decrease variation, reduce chemical exposure--the benefit list is endless. But shouldn't we all at least KNOW what these machines are doing for us? My two cents... Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 Sign up for the FREE e-newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Lab. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From JMyers1 <@t> aol.com Fri Feb 2 12:17:33 2007 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Feb 2 12:17:48 2007 Subject: RE [Histonet] A question...(about IHC buffer) Message-ID: The primary purpose of placing slides in buffer (for a short time) prior to IHC staining is to equilibrate the specimen -- that is, create a suitable environment for the immunologic reagents to interact as they would in nature. Although the first reagent that is applied may work just fine without the pre-soak step, its considered 'good science' to equilibrate beforehand... Joe Myers, M.S., CT(ASCP) ----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rosa Bryant Sent: Friday, February 02, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question.. What is the science behind soaking the slides in buffer before IHC procedures? I can't find much of a reference about this, what are the advantages to this step instead of putting the slides back into water? Thanks Rosa Fields From rjbuesa <@t> yahoo.com Fri Feb 2 12:23:19 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 2 12:23:26 2007 Subject: [Histonet] Losing our art form--manual vs. automated In-Reply-To: <003d01c746e6$9abe3e90$6401a8c0@FSDESKTOP> Message-ID: <423673.30992.qm@web61225.mail.yahoo.com> Those in that real story are happy they did not work under my supervisoin, because they would have been fired on the spot, NOT for NOT knowing how to hand-coverslip, but for the lack of initiative and not even bothering to look for the supervisor and ask what to do. They chose to iddle and enjoy payment witout work. Absolutely disgusting and unacceptable! Ren? J. Cheryl Kerry wrote: Did you all hear the story (true) about the group of newly certified techs and the cover slips? One day the block load was larger than usual and, of course, the coverslipper broke. The newly certified techs were standing around, hands in pockets, because they couldn't turn out the slides. When the old-school manager came in and asked what the holdup was, they responded "We can't turn anything out--they aren't coverslipped." Flabbergasted, the manager donned gloves, sat down and showed them how to apply coverslips the old fashioned way. ________________ I interview 5 or 6 techs a day. I'm finding many have never had the experience the fine art of the hand-stained H&E. I know these machines save time, increase production, decrease variation, reduce chemical exposure--the benefit list is endless. But shouldn't we all at least KNOW what these machines are doing for us? My two cents... Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 Sign up for the FREE e-newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Lab. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From pruegg <@t> ihctech.net Fri Feb 2 12:57:57 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Feb 2 12:58:29 2007 Subject: [Histonet] Histogel and microwave processing In-Reply-To: Message-ID: <003b01c746fc$101cbc10$6501a8c0@Patsy> I don't know about mw processing of histogel cell blocks but I use a very short cycle of 20 min per station starting with 70% etoh after the cell block is well fixed which comes down to about 3.5 hrs. processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KirkyDog2@aol.com Sent: Friday, February 02, 2007 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histogel and microwave processing Hello everyone, I need help if anyone is willing. We currently process our FNA cell blocks in "Histogel" using an overnight cycle, our cytopathologist would like his results faster. Does anyone know if histogel is compatible with microwave processing techniques, and if the cellular morphology is compromised in any way with such a method? Thank you in advance for your input. T. Kirk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Feb 2 13:01:56 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Feb 2 13:02:08 2007 Subject: [Histonet] Histogel and microwave processing References: Message-ID: We have the Sakura Xpress and the Histogel processes fine. If you have an Xpress just be sure you use a minimal time in the pre-processing solution as the morphology changes slightly if there is over-use. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KirkyDog2@aol.com Sent: Friday, February 02, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histogel and microwave processing Hello everyone, I need help if anyone is willing. We currently process our FNA cell blocks in "Histogel" using an overnight cycle, our cytopathologist would like his results faster. Does anyone know if histogel is compatible with microwave processing techniques, and if the cellular morphology is compromised in any way with such a method? Thank you in advance for your input. T. Kirk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From proctoth <@t> ohsu.edu Fri Feb 2 13:05:25 2007 From: proctoth <@t> ohsu.edu (Thomas Proctor) Date: Fri Feb 2 13:05:53 2007 Subject: [Histonet] Losing our art form--manual vs. automated Message-ID: I do not have any automated equipment; I routinely process paraffin and plastic resin and frozen blocks by hand, from dissection to sections to stains to micrographs. Although I have over 5 years experience as a lab tech, I just started a new position running a histology core for an exclusive group of five investigators. At times, it definately seems much more like art than science! I've never used automated equipment, and I'm sure the benefits are great. But I don't like the idea of not being in control, e.g. when machines break... Those in that real story are happy they did not work under my supervisoin, because they would have been fired on the spot, NOT for NOT knowing how to hand-coverslip, but for the lack of initiative and not even bothering to look for the supervisor and ask what to do. They chose to iddle and enjoy payment witout work. Absolutely disgusting and unacceptable! Ren? J. Cheryl Kerry wrote: Did you all hear the story (true) about the group of newly certified techs and the cover slips? One day the block load was larger than usual and, of course, the coverslipper broke. The newly certified techs were standing around, hands in pockets, because they couldn't turn out the slides. When the old-school manager came in and asked what the holdup was, they responded "We can't turn anything out--they aren't coverslipped." Flabbergasted, the manager donned gloves, sat down and showed them how to apply coverslips the old fashioned way. ________________ I interview 5 or 6 techs a day. I'm finding many have never had the experience the fine art of the hand-stained H&E. I know these machines save time, increase production, decrease variation, reduce chemical exposure--the benefit list is endless. But shouldn't we all at least KNOW what these machines are doing for us? My two cents... Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 Sign up for the FREE e-newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Lab. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From pruegg <@t> ihctech.net Fri Feb 2 13:08:15 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Feb 2 13:08:30 2007 Subject: [Histonet] Histogel and microwave processing In-Reply-To: <003b01c746fc$101cbc10$6501a8c0@Patsy> Message-ID: <004101c746fd$7e9ff1b0$6501a8c0@Patsy> Actually I would worry about mw processing of histogel cell blocks as they liquefy when heated. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, February 02, 2007 11:58 AM To: KirkyDog2@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histogel and microwave processing I don't know about mw processing of histogel cell blocks but I use a very short cycle of 20 min per station starting with 70% etoh after the cell block is well fixed which comes down to about 3.5 hrs. processing. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KirkyDog2@aol.com Sent: Friday, February 02, 2007 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histogel and microwave processing Hello everyone, I need help if anyone is willing. We currently process our FNA cell blocks in "Histogel" using an overnight cycle, our cytopathologist would like his results faster. Does anyone know if histogel is compatible with microwave processing techniques, and if the cellular morphology is compromised in any way with such a method? Thank you in advance for your input. T. Kirk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hkromer <@t> KaiserAssociates.com Fri Feb 2 13:19:40 2007 From: hkromer <@t> KaiserAssociates.com (Heather Kromer) Date: Fri Feb 2 13:13:38 2007 Subject: [Histonet] Last Chance! $50-150 honorarium Message-ID: Dear Histonnetters, This is your last chance to participate in this survey and make $50-150 (please do not respond if you have already). Our firm, Kaiser Associates, is conducting research on Advanced Staining processes, and would like your input. By participating in a survey you will be influencing potential upcoming changes to the market for Advanced Staining products. The completion of this survey should take only 25 minutes, and in return for your participation you will receive an honorarium of US $50 -150.00, depending on your qualifications. If you would like to participate in this survey please reply to Hkromer@kaiserassociates.com with your job title and company/hospital name and we will send you the weblink to the survey. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research based international consulting firm, dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com From bads27 <@t> msn.com Fri Feb 2 13:48:42 2007 From: bads27 <@t> msn.com (BETH DELESCAVAGE) Date: Fri Feb 2 13:48:54 2007 Subject: [Histonet] Nuclear Fast Red Message-ID: Hello All- We are looking for a new Nuclear Fast Red. The one we are currently using is from J.T. Baker (cat#S635-01 lot#X49673), when put into solution the liquid looks like grape juice instead of the little red/pink we are used to seeing. Can anyone recommend a good brand? We have remade the solution twice to insure it was not a heat or a reagent issue, we are sure it is the brand. Thank you. -Beth Delescavage, BS, HTL (ASCP) PhenoPath Labs From MSHERWOOD <@t> PARTNERS.ORG Fri Feb 2 14:14:47 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Feb 2 14:15:10 2007 Subject: [Histonet] Nuclear Fast Red In-Reply-To: Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30F0F@PHSXMB1.partners.org> We use Nuclear Fast Red (Kernechtrot 0.1%) from Poly Scientific (Bay Shore, NY)--already to use. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of BETH DELESCAVAGE Sent: Friday, February 02, 2007 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Fast Red Hello All- We are looking for a new Nuclear Fast Red. The one we are currently using is from J.T. Baker (cat#S635-01 lot#X49673), when put into solution the liquid looks like grape juice instead of the little red/pink we are used to seeing. Can anyone recommend a good brand? We have remade the solution twice to insure it was not a heat or a reagent issue, we are sure it is the brand. Thank you. -Beth Delescavage, BS, HTL (ASCP) PhenoPath Labs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. From anitathorn <@t> comcast.net Fri Feb 2 14:16:10 2007 From: anitathorn <@t> comcast.net (anitathorn@comcast.net) Date: Fri Feb 2 14:16:18 2007 Subject: [Histonet] Italy Message-ID: <020220072016.25645.45C39C09000EE9BD0000642D2213575333029D01089B0E9B07020E@comcast.net> Hi everyone, Does anyone out there work in a histology lab in Italy or know someone who does that I can correspond with (preferably in English!) From mcauliff <@t> umdnj.edu Fri Feb 2 14:22:19 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 2 14:22:36 2007 Subject: [Histonet] Nuclear Fast Red In-Reply-To: References: Message-ID: <45C39D7B.40809@umdnj.edu> Instead of NFR I suggest you try "Scarba Red", much more intense red/orange stain made with neutral red, analine and phenol. See Slidders et al, J. Pathol. Bact. 75:476-478, 1958. If you like I can send you the recipe. Geoff BETH DELESCAVAGE wrote: > Hello All- > We are looking for a new Nuclear Fast Red. The one we are currently > using is from J.T. Baker (cat#S635-01 lot#X49673), when put into > solution the liquid looks like grape juice instead of the little > red/pink we are used to seeing. Can anyone recommend a good brand? > We have remade the solution twice to insure it was not a heat or a > reagent issue, we are sure it is the brand. Thank you. > -Beth Delescavage, BS, HTL (ASCP) > PhenoPath Labs > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From PMonfils <@t> Lifespan.org Fri Feb 2 14:40:46 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Feb 2 14:40:53 2007 Subject: [Histonet] Nuclear Fast Red In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C17@LSRIEXCH1.lsmaster.lifespan.org> I get nuclear fast red, and many of my other stains, from Rowley Biochemical Institute. Histological stains and dyes are their business, and I have never been dissatisfied with their products. http://www.rowleybio.com > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of BETH DELESCAVAGE > Sent: Friday, February 2, 2007 11:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Nuclear Fast Red > > Hello All- > We are looking for a new Nuclear Fast Red. The one we are currently using > is from J.T. Baker (cat#S635-01 lot#X49673), when put into solution the > liquid looks like grape juice instead of the little red/pink we are used to > seeing. Can anyone recommend a good brand? We have remade the solution > twice to insure it was not a heat or a reagent issue, we are sure it is the > brand. Thank you. > -Beth Delescavage, BS, HTL (ASCP) > PhenoPath Labs > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JWilkinson <@t> adclinic.com Fri Feb 2 14:50:46 2007 From: JWilkinson <@t> adclinic.com (Wilkinson, Joyce E) Date: Fri Feb 2 14:51:27 2007 Subject: [Histonet] starting a new lab Message-ID: Hi I'm starting up a brand new Histology lab in a clinic ( I have never done this before) serving approx. 120 physicians and an outpatient surgery center. I will be doing mostly biopsies, skins, bone marrows and our largest specimen will be a gallbladder from the out patient surgery center. I will be using a refurbish Sakura Tissue-Tek VIP E150 processor. My question is how do I set up the processor. I would like the end time to be approx. 4:00 am. How many xylenes, alcohols etc. Also, set up for a short run, what could be the shortest time for a biopsy, starting in formalin. What kind of paraffin? Thanks Joyce From froyer <@t> bitstream.net Fri Feb 2 15:01:51 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Feb 2 15:02:26 2007 Subject: [Histonet] starting a new lab In-Reply-To: References: Message-ID: <001801c7470d$5ca28450$7701a80a@Ford> Joyce, Did you not receive an Operator's Manual with the refurbished VIP E-150? There was also a Training Manual that went with this model. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wilkinson, Joyce E Sent: Friday, February 02, 2007 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] starting a new lab Hi I'm starting up a brand new Histology lab in a clinic ( I have never done this before) serving approx. 120 physicians and an outpatient surgery center. I will be doing mostly biopsies, skins, bone marrows and our largest specimen will be a gallbladder from the out patient surgery center. I will be using a refurbish Sakura Tissue-Tek VIP E150 processor. My question is how do I set up the processor. I would like the end time to be approx. 4:00 am. How many xylenes, alcohols etc. Also, set up for a short run, what could be the shortest time for a biopsy, starting in formalin. What kind of paraffin? Thanks Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Fri Feb 2 15:02:49 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Feb 2 15:03:00 2007 Subject: [Histonet] Nuclear Fast Red In-Reply-To: References: Message-ID: Vector labs. We buy premade. It's very good. >Hello All- >We are looking for a new Nuclear Fast Red. The one we are currently >using is from J.T. Baker (cat#S635-01 lot#X49673), when put into >solution the liquid looks like grape juice instead of the little >red/pink we are used to seeing. Can anyone recommend a good brand? >We have remade the solution twice to insure it was not a heat or a >reagent issue, we are sure it is the brand. Thank you. >-Beth Delescavage, BS, HTL (ASCP) >PhenoPath Labs -- From godsgalnow <@t> aol.com Fri Feb 2 15:13:15 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Feb 2 15:13:28 2007 Subject: [Histonet] starting a new lab In-Reply-To: References: Message-ID: <8C9152616A50854-180C-2534@WEBMAIL-RA17.sysops.aol.com> Joyce, There are many variations to programs out there, it depends on if you want to use formalin or formalin substitutes, xylene or xylene substitues, etc. But typically, for a normal run depending on how thick the skins are cut in (I personally would do bone marrow on a separate run omitting the formalins, but that is my bias). Here is my opinion: formalin 1 hour formalin 1 hour 70 % alcohol 45 minutes 95 % alcohol 1 hour 95 % alcohol 45 minutes 100 % alcohol 45 minutes 100 % alcohol 1 hour 1/2 100 % alcohol % 1/2 xylene 45 minutes xylene 45 minutes xylene 1 hour paraplast plus 45 minutes at 59 degrees paraplast plus 45 minutes ditto paraplast plus 45 minutes ditto for a biopsy run I would do the same thing but cut the time down to 15 - 20 minutes per station If I can be of any other help, let me know...... Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa -----Original Message----- From: JWilkinson@adclinic.com To: histonet@lists.utsouthwestern.edu Sent: Fri, 2 Feb 2007 3:50 PM Subject: [Histonet] starting a new lab Hi I'm starting up a brand new Histology lab in a clinic ( I have never done this before) serving approx. 120 physicians and an outpatient surgery center. I will be doing mostly biopsies, skins, bone marrows and our largest specimen will be a gallbladder from the out patient surgery center. I will be using a refurbish Sakura Tissue-Tek VIP E150 processor. My question is how do I set up the processor. I would like the end time to be approx. 4:00 am. How many xylenes, alcohols etc. Also, set up for a short run, what could be the shortest time for a biopsy, starting in formalin. What kind of paraffin? Thanks Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From ahaines <@t> vims.edu Fri Feb 2 15:19:58 2007 From: ahaines <@t> vims.edu (ahaines@vims.edu) Date: Fri Feb 2 15:20:21 2007 Subject: [Histonet] Causes of antigen masking (IF on sections vs. flow cytometry) Message-ID: <20070202161958.ANJ10108@mir450.vims.edu> This may be in part a philosophical debate, but... There are some mixed messages in the literature (both scientific and protocol manuals) about the cause of antigen masking. Most state that it is caused by fixation (for example see below), but others include paraffin embedding as a cause as well. I rather thought that the problem with embedding might be antigen loss due to heat rather than masking, although with low melting point paraffin it shouldn't be a big contibutor... Anyway, here's the question: We are sucessfully doing immunonostaining for an intrancellular antigen on cell suspensions for flow cytometry. Preparation steps include ~2% paraformaldehyde fixation and permeabilization by methanol. Next we hope to localize our positive cells within the organ (kidney) by doing immunofluorescence on tissue sections. Would you agree that, based on our success with staining cell suspensions, we should not expect to encounter problems with antigen masking in the tissue sections? Is paraffin embedding likely to contribute to antigen loss/masking? Alternatively, does the thickness of a paraffin section compared to a cell suspension greatly influence the ability to permeabilize? In theory the ethanol steps in embedding and deparaffinizing should more than adequately permeabilize the cells... Your thoughts would be appreciated. Ashley From: Phyllis Davie Date: Fri, 02 Mar 2001 11:54:54 -0800 To: Gerard Spoelstra , Subject: Re: Antigen Retreival Solution Enclosed please find our methods for antigen retrieval. We use several different methods. Please feel free to contact me if you have any questions. Best of luck, Phyllis Davie PhenoPath Laboratories--Seattle, WA pdavie@phenopath.com PRETREATMENT, ANTIGEN RETRIEVAL HEAT INDUCED EPITOPE RETRIEVAL (HIER) Purpose: The Heat Induced Epitope Retrieval (HIER) procedure is used to unmask or retrieve epitopes of poorly fixed or over-fixed tissues, as well as unmask epitopes in previously non-reactive tissue. It is especially useful in formalin fixed tissues. Formalin fixes tissues by blocking amido groups and by forming methylene bridges between several amino acids in polypeptides. By this "cross-linking" mechanism, formalin effects a variable number of chemical links not only within a protein molecule but also with adjacent proteins. These cross-linked proteins are believed to block access of antibodies to their target epitopes, a process often called "masking" of antigens. Although the exact mechanism by which HIER unmasks epitopes is unknown, many studies have demonstrated improved immunoreactivity of tissue sections following HIER. From themagoos <@t> rushmore.com Fri Feb 2 15:28:39 2007 From: themagoos <@t> rushmore.com (themagoos) Date: Fri Feb 2 15:28:44 2007 Subject: [Histonet] Position opening for HT/HTL or eligible Message-ID: <45c3ad07.3f.f16.1494501511@rushmore.com> see attached ad From proctoth <@t> ohsu.edu Fri Feb 2 15:38:11 2007 From: proctoth <@t> ohsu.edu (Thomas Proctor) Date: Fri Feb 2 15:38:45 2007 Subject: [Histonet] slides for paraffin Message-ID: I would greatly appreciate some input on the best brand and/or type of microscope slides to use for paraffin sections, that reduces the incidence of secitons peeling off of the slide during staining and processing; I typically do H & E, LFB/PAS, and immunostaining, and my sections are typically 7 microns. Thank you. Thomas From themagoos <@t> rushmore.com Fri Feb 2 16:32:04 2007 From: themagoos <@t> rushmore.com (themagoos) Date: Fri Feb 2 16:32:09 2007 Subject: [Histonet] Position opening for HT/HTL or eligible Message-ID: <45c3bbe4.186.3652.2034100854@rushmore.com> Clinical Laboratory of the Black Hills is a growing, independent pathology practice providing AP services to Rapid City, South Dakota and surrounding communities. Rapid City is the gateway to the Black Hills and offers a variety of four season, family friendly activities. Our histology department processes over 26,000 surgical cases, over 200 autopsies and an additional 16,000 dermatology specimens per year. We also have a progressive IHC department, and perform a variety of special stains and frozen sections. HISTOTECH Immediate opening for a HT/HTL (ASCP) or eligible. Experience in all technical aspects of routine histology and immunohistochemical stains preferred. Associate Degree in related field a plus.F/T - Day shifts only. Competitive wage and excellent benefit package No state income tax Relocation assistance available Immediate 401K participation Send resume to: Robin Mutschelknaus, HT (ASCP) Histology Supervisor Clinical Laboratory of the Black Hills 2805 5th Street, Suite 210 Rapid City, South Dakota 57701 Fax: 605-342-0418; Phone: 605-343-2267 Email: rmutschelknaus@clinlab.com From carl.hobbs <@t> kcl.ac.uk Fri Feb 2 16:38:02 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Feb 2 16:38:24 2007 Subject: [Histonet] re: hypoxyprobe Message-ID: <007a01c7471a$ce7a1ea0$4101a8c0@carlba65530bda> the hypoxia story: you give the patient iv pimonidazole (the hypoxia marker). after some hours you take the biopsy and you add the antibody that recognizes the pimonizadole bound. if there is no pimonidazole there is no reason to proceed with the antibody. these chaps have a brain biopsy material and they want to see if there is hypoxia. i am afraid is not possible without the soministration of pmn before the biopsy. That's from a Great Reseacher who I am working with From mrsseagle <@t> yahoo.com Fri Feb 2 17:44:36 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Fri Feb 2 17:44:47 2007 Subject: [Histonet] Sentinel node protocol? Message-ID: <351317.23495.qm@web51809.mail.yahoo.com> I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill. thanks Michelle Seagle Rutherford Hospital HT (ASCP) --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From conniegrubaugh <@t> hotmail.com Fri Feb 2 18:12:39 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Feb 2 18:12:53 2007 Subject: [Histonet] HT/HTL practical exam - different opinion In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D324B@sjhaexc02.sjha.org> Message-ID: Hear Hear!!!!! I agree with you. Thanks for making it short, sweet and to the point. Connie Grubaugh Las Vegas Nv. >From: "Weems, Joyce" >To: , >, >Subject: RE: [Histonet] HT/HTL practical exam - different opinion >Date: Fri, 2 Feb 2007 10:34:37 -0500 > >I feel a need to add my 2 cents... > >We have whined for years about not being considered as professional as >Med Techs... > >Not having to do a practical is a step closer to being treated the same. > > >Joyce > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital of Atlanta >404-851-7376 > > >Confidentiality Notice ** The information contained in this message may be >privileged and is confidential information intended for the use of the >addressee listed above. If you are neither the intended recipient nor the >employee or agent responsible for delivering this message to the intended >recipient, you are hereby notified that any disclosure, copying, >distribution or the taking of any action in reliance on the contents of >this information is strictly prohibited. If you have received this >communication in error, please notify us immediately by replying to the >message and deleting it from your computer. Thank you. Saint Joseph's >Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get in the mood for Valentine's Day. View photos, recipes and more on your Live.com page. http://www.live.com/?addTemplate=ValentinesDay&ocid=T001MSN30A0701 From jnocito <@t> satx.rr.com Fri Feb 2 19:02:14 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Feb 2 19:02:37 2007 Subject: [Histonet] ASCP Exam Long opinion References: Message-ID: <011701c7472e$f2b05aa0$649eae18@yourxhtr8hvc4p> I agree with you Barry. Techs need to know how the procedure is working to be able to troubleshoot when something happens. Is it the machine or the procedure? That's what I stressed when I was giving my intro to IHC lectures. Something intricate like immunos should be done manually until the tech is familiar with the procedure before they put slides on a machine. But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, I think I'll have a scotch. Enjoy Joe ----- Original Message ----- From: "Rittman, Barry R" To: "Edwards, R.E." ; "Jasper, Thomas G." Cc: Sent: Friday, February 02, 2007 8:59 AM Subject: RE: [Histonet] ASCP Exam Long opinion Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Feb 2 22:28:54 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Feb 2 22:29:13 2007 Subject: [Histonet] Re: Nuclear Fast Red Message-ID: Beth Delescavage asks about sources for nuclear fast red. Nuclear fast red is a dye whose future is pretty uncertain. Anatech offers an alternative, "Brazilliant", brazilin in alum. Brazilin is a natural dye botanically and chemically related to hematoxylin, but it's red. Has anybody on the list tried this stuff? With what results? (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN From RSRICHMOND <@t> aol.com Fri Feb 2 22:39:08 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Feb 2 22:39:32 2007 Subject: [Histonet] Re: Sentinel node protocol Message-ID: Michelle Seagle at Rutherford Hospital (where?) asks:?>>I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill.<< This topic is very controversial and very little standardized. The service I just left did a great many of these, many breast cases, many with multiple sentinel nodes. They did one H & E, with S100 and MELAN-A for melanoma, and cytokeratin AE1/AE3 for breast and squamous carcinoma. Unfortunately, frozen sections were also performed, more often than not. My personal preference would be no frozen section (except for nodes grossly positive on gross examination, which should be done with great care), overnight fixation before processing, with the slides as above, and the histotechnologist checking them for adequacy before handing them to me. If Michelle Seagle's question includes how to deal with the radioactivity of the specimen: ignore it. Bob Richmond Samurai Pathologist Knoxville TN From rjbuesa <@t> yahoo.com Sat Feb 3 06:42:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 3 06:42:37 2007 Subject: [Histonet] Sentinel node protocol? In-Reply-To: <351317.23495.qm@web51809.mail.yahoo.com> Message-ID: <811041.97172.qm@web61211.mail.yahoo.com> Michelle: Not knowing what do you refer to as "over kill" I cannot commento on your particular protocol BUT on the other hand I do think that being as thorough as possible with all LN is worth the "troub;e" for the patient involved. Ren? J. MICHELLE SEAGLE wrote: I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill. thanks Michelle Seagle Rutherford Hospital HT (ASCP) --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From JWEEMS <@t> sjha.org Sat Feb 3 09:11:17 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Feb 3 09:11:47 2007 Subject: [Histonet] Re: Sentinel node protocol Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3256@sjhaexc02.sjha.org> If I understand this procedure correctly it was originally intended to eliminate further surgery at the time of the procedure by doing the frozen section on the sentinel node. Rapid immunos were promoted. If there was not malignancy, no further suregery would be done. Then, fear of the radioactive component became a problem and somehow it become distorted as to what is required. In my opinion, frozens do not need to be done unless it will eliminate further surgery. It is just extra cost and may compromise immunos for the permanent section. We do not do frozens. We do the cytokeratin immunos for breast and melanoma panel for melanomas. I have not tracked statistics for how many require more surgery. This seems to be a procedure that could use standardization, instead of the government trying to eliminate payment for biopsies that need separate diagnoses. (MUEs) In my humble opinion.... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Friday, February 02, 2007 11:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sentinel node protocol Michelle Seagle at Rutherford Hospital (where?) asks:?>>I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill.<< This topic is very controversial and very little standardized. The service I just left did a great many of these, many breast cases, many with multiple sentinel nodes. They did one H & E, with S100 and MELAN-A for melanoma, and cytokeratin AE1/AE3 for breast and squamous carcinoma. Unfortunately, frozen sections were also performed, more often than not. My personal preference would be no frozen section (except for nodes grossly positive on gross examination, which should be done with great care), overnight fixation before processing, with the slides as above, and the histotechnologist checking them for adequacy before handing them to me. If Michelle Seagle's question includes how to deal with the radioactivity of the specimen: ignore it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From asmith <@t> mail.barry.edu Sat Feb 3 11:13:40 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sat Feb 3 11:13:48 2007 Subject: [Histonet] Re: Nuclear Fast Red In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E403F@exchsrv01.barrynet.barry.edu> I have not used Anatech's "Brazilliant", but I have used homemade alum brazilin with good results. I assume that Anatech's premix would be as good or better than my home brew. Because the stain is not as dense as hematoxylin, it is very good for thick sections. Nuclear detail is a little sharper than what one gets with nuclear faxt red. My home brewed brazillin is not completely selective for nuclei: it stains keratin and stains collagen lightly. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Friday, February 02, 2007 11:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Nuclear Fast Red Beth Delescavage asks about sources for nuclear fast red. Nuclear fast red is a dye whose future is pretty uncertain. Anatech offers an alternative, "Brazilliant", brazilin in alum. Brazilin is a natural dye botanically and chemically related to hematoxylin, but it's red. Has anybody on the list tried this stuff? With what results? (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From gu.lang <@t> gmx.at Sat Feb 3 12:38:56 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 3 12:39:04 2007 Subject: AW: [Histonet] Sentinel node protocol? In-Reply-To: <351317.23495.qm@web51809.mail.yahoo.com> Message-ID: <001301c747c2$919b9ab0$eeeea8c0@dielangs.at> Last year we started a program for the breast sentinels with serial cuts in 50 ?m steps. The first and the last were HE stained, the others Pancytokeratin. The pathologists wanted to see, if there is any significant differenc in the results to our former protocol with 250?m steps. Now we turned again to the larger distance. We also do frozens on the half part of the sentinel if it is smaller than 5 mm, and on the middle 2mm-part if it is larger. We perform also one slide as rapid ck-pan. But for all good reasons to get the information of the frozen, we cannot be sure about the result until the last immuno-slide. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von MICHELLE SEAGLE Gesendet: Samstag, 03. Februar 2007 00:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sentinel node protocol? I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill. thanks Michelle Seagle Rutherford Hospital HT (ASCP) --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Feb 3 14:05:18 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Feb 3 14:05:43 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: <011701c7472e$f2b05aa0$649eae18@yourxhtr8hvc4p> Message-ID: <200702032005.l13K5CC3070217@pro12.abac.com> I think I am even more of a dinosaur than you Joe. I think all the automation is taking us farther and farther away from connecting with the patient which is after all why we do what we do. I came from a unique situation, early in my histology career I took a job at the U of Colorado with Dr. Matthew Block in the department of Hematology/Oncology. Dr. Block was a practicing hematologist/oncologist who also became a board certified Pathologist and set up his own histology lab because he did not like the service he got from the pathology/histology department. He literally treated the patient and did his own path processing and report. We would go with Dr. Block to the patient and assist with taking a bone marrow biopsy or lymph node biopsy, take it back to the lab, process it into GMA plastic in those days because Dr. Block wanted really thin tissue sections. He was a hematologist who looked at everything under oil immersion and insisted on impeccable cell morphology. We saw the faces of the people we were preparing tissue sections for. I remember helping mothers hold babies while bone marrow was taken. I think these experiences continue to remind me that there is a patient whose parts we are attending to. I know this experience is not common and certainly not practical anymore but I think the more and more we push buttons and don't look at our slides the farther we are removed from remembering that there are patients we need to do our best for. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, February 02, 2007 6:02 PM To: Rittman, Barry R; Edwards, R.E.; Jasper, Thomas G. Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Exam Long opinion I agree with you Barry. Techs need to know how the procedure is working to be able to troubleshoot when something happens. Is it the machine or the procedure? That's what I stressed when I was giving my intro to IHC lectures. Something intricate like immunos should be done manually until the tech is familiar with the procedure before they put slides on a machine. But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, I think I'll have a scotch. Enjoy Joe ----- Original Message ----- From: "Rittman, Barry R" To: "Edwards, R.E." ; "Jasper, Thomas G." Cc: Sent: Friday, February 02, 2007 8:59 AM Subject: RE: [Histonet] ASCP Exam Long opinion Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpgarcia <@t> salk.edu Sat Feb 3 19:35:05 2007 From: cpgarcia <@t> salk.edu (Carlos G. Perez-Garcia) Date: Sat Feb 3 19:35:12 2007 Subject: [Histonet] peptide-blocking Message-ID: <7BDD827B-AF0D-49C4-9C52-46D43BF326CF@salk.edu> hi everybody i need to do a peptide-antibody blocking experiment to check the specificity of an antibody i made. i wonder if somebody has experience on that. i suppose that just adding 5-10 times more peptide than antibody in the solution will be enough right? does anybody have any protocol or suggestion? thanks in advance Carlos Carlos G. Perez-Garcia, Ph.D. From lpaveli1 <@t> hurleymc.com Sat Feb 3 21:41:39 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Sat Feb 3 21:42:21 2007 Subject: [Histonet] Re: Nuclear Fast Red Message-ID: <45C50FA4020000EE00011055@smtp-gw.hurleymc.com> I have used "Brazilliant" for a few years, and has given good nuclear staining. No complaints from the docs. Lynette >>> 02/02/07 11:28 PM >>> Beth Delescavage asks about sources for nuclear fast red. Nuclear fast red is a dye whose future is pretty uncertain. Anatech offers an alternative, "Brazilliant", brazilin in alum. Brazilin is a natural dye botanically and chemically related to hematoxylin, but it's red. Has anybody on the list tried this stuff? With what results? (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sun Feb 4 09:46:01 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Feb 4 09:48:32 2007 Subject: [Histonet] ASCP Exam Long opinion References: <200702032005.l13K5CC3070217@pro12.abac.com> Message-ID: <006601c74873$941dac40$649eae18@yourxhtr8hvc4p> Patsy, I am closer to you than you think. At my first lab after histology school at Keesler AFB, MS, I had to assist on bone marrows and worked in the blood drawing room when I had no pathologist. I saw how bone marrows were performed and it scared me to death when I had to embed and cut the blocks. I didn't not want to mess it up and then have the patient return for another procedure because of my mistake. There were many retirees in Miami that were getting cancer treatment at the base. Often, the regular patients would request me to draw their blood because I also joked with them and was able to get the blood on the first try. I remember one lady was trying to fix me up with her granddaughter, a total knockout. She had told her granddaughter so much about me that the granddaughter was coming done for the summer. Unfortunately, the grandmother succumbed to cancer and I never had the chance to meet her. The attending wanted an autopsy. I was the only tech so I had to assist on it. When the pathologist and I were about to start, I got teary eyed. The doc asked what was up, so I told him. He told me to go back to the lab and he would get someone else to put her back in the cooler when he was done, all I had to do was clean up. Number 1= you are right, we just don't have the patient interaction we used to. It is hard for tech to try in put a face with the block. Still, to this day, 3 years later, I remember those experiences. Experiences that students today may or may not experience. Number 2= some pathologists today would not have understood and would tell me to suck it up. Today it's more of a turn around time/insurance payment issue than ever. Joe ----- Original Message ----- From: "patsy ruegg" To: "'Joe Nocito'" ; "'Rittman, Barry R'" ; "'Edwards, R.E.'" ; "'Jasper, Thomas G.'" Cc: Sent: Saturday, February 03, 2007 2:05 PM Subject: RE: [Histonet] ASCP Exam Long opinion >I think I am even more of a dinosaur than you Joe. > I think all the automation is taking us farther and farther away from > connecting with the patient which is after all why we do what we do. > I came from a unique situation, early in my histology career I took a job > at > the U of Colorado with Dr. Matthew Block in the department of > Hematology/Oncology. Dr. Block was a practicing hematologist/oncologist > who > also became a board certified Pathologist and set up his own histology lab > because he did not like the service he got from the pathology/histology > department. He literally treated the patient and did his own path > processing and report. We would go with Dr. Block to the patient and > assist > with taking a bone marrow biopsy or lymph node biopsy, take it back to the > lab, process it into GMA plastic in those days because Dr. Block wanted > really thin tissue sections. He was a hematologist who looked at > everything > under oil immersion and insisted on impeccable cell morphology. We saw > the > faces of the people we were preparing tissue sections for. I remember > helping mothers hold babies while bone marrow was taken. I think these > experiences continue to remind me that there is a patient whose parts we > are > attending to. I know this experience is not common and certainly not > practical anymore but I think the more and more we push buttons and don't > look at our slides the farther we are removed from remembering that there > are patients we need to do our best for. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Friday, February 02, 2007 6:02 PM > To: Rittman, Barry R; Edwards, R.E.; Jasper, Thomas G. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP Exam Long opinion > > I agree with you Barry. Techs need to know how the procedure is working to > be able to troubleshoot when something happens. Is it the machine or the > procedure? That's what I stressed when I was giving my intro to IHC > lectures. Something intricate like immunos should be done manually until > the > > tech is familiar with the procedure before they put slides on a machine. > But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, > I > > think I'll have a scotch. Enjoy > > Joe > ----- Original Message ----- > From: "Rittman, Barry R" > To: "Edwards, R.E." ; "Jasper, Thomas G." > > Cc: > Sent: Friday, February 02, 2007 8:59 AM > Subject: RE: [Histonet] ASCP Exam Long opinion > > > Nothing is black and white nowadays and I think that the current > discussion on Histonet is important in putting forward all points of > view.. > > I agree that automation is very important for producing consistent > results and in many ways these have relieved us of tedious, repetitive > tasks. > I also respect the supervisors who are often stuck between a rock and a > hard place. > > The problem I currently see is that in many cases these machines may be > operated in a robotic fashion. If the individual operating the machine > is knowledgeable about the process taking place be it histochemical, > immunochemical or special stains and can take appropriate steps if > problems arise then there should be no problem. > Unfortunately in today's market, not just for histology, the emphasis > appears to be on minimal qualifications to carry out a task. This may > work perfectly well in those cases where instructions are concise and > easily followed and solutions changed at specific intervals and times > adhered to. > However, I believe something is lost to the histotechs in such a > situation. > > After all I operate my car with minimal knowledge of the mechanics and > electronics. However were I to get paid to operate my car I would hope > that I had time and make the effort to try understand the processes > involved. I would consider this part of responsibilities and also a > bonus in enhancing my work experience. > My point is, are we going to "progress" into an era where we are just > button pushers? > This automation also releases individual so that many histotechs may > carry out tasks that never used to be their responsibility. > This appears to be a national trend. As an example, I am paid as a > faculty member here but my time spent includes memos, filing etc. a task > originally carried out by secretarial staff. It is not that I resent > doing such tasks but after all I am paid more than secretaries and the > "secretarial tasks" never appear anywhere in my yearly report of > activities. > > Perhaps we will end up with histotechs plus a second group of button > pushers? Perhaps secretaries will be reincarnated - probably not. > > I guess that I am from an era where work was varied and pleasurable > rather than one in which the bottom line was the aim, and where employee > and employees worked as a team. > Hoe that this makes sense, this has been a one coffee morning so far. > Barry > > > -----Original Message----- > From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] > Sent: Friday, February 02, 2007 8:09 AM > To: Jasper, Thomas G.; Rittman, Barry R > Cc: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP Exam Long opinion > > Laboratory automation surely is as much due to obtaining > reproducibility of results than saving on staff costs, as to date > at least, all machines need human minders, who earn their corn > when the machine malfunctions and for example they have to do a > batch of H@Es by hand. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, > Thomas G. > Sent: 01 February 2007 18:17 > To: Rittman, Barry R > Cc: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP Exam Long opinion > > Hey Barry, > > I appreciate your long opinion, at times life requires long opinions. I > agree with much of what you say and I will try to concisely explain how > I see things. I totally agree that elimination of the practical was a > bad idea. And I understand the arguments for getting rid of the > practical. Let's start with automated staining. Unfortunately most > labs in the clinical world of the US, Can., UK, Western Europe, S. > Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned > to this technology out of necessity. This is due to staff shortages and > for cost containment. The consistency and reproducibility are greatly > enhanced by reduction of variables (I know more science than art). But > there you are, so a lab's automated stainer produces a stain, candidates > submit. If this is the technology a candidate will likely use, that > should be taken into consideration. > I expect a candidate to cut their own sections. That would be subject > to evaluation as it always was. And I expect someone to know what an H > and E or any other stain is supposed to look like, automated or not. > With so much emphasis on academics they should know what the stain looks > like and why. Heck, with all this automation you could even expect a > basic level of understanding about the mechanics involved and make that > part of the written exam. > Perhaps the elimination of the practical was not necessary, but a > reassessment of the whole thing, or as you so cleverly stated "...what > is needed for the entire system is a good enema!" I agree with the > statements from others that they want to know that folks who are > certified can cut sections. It is more balanced and despite all of our > wonderful automation Histology is the one laboratory discipline that > still requires a deft hand and an artistic eye. I'm not aware of any > automated substitute for manual dexterity. > I also agree with this whole ASCP/fox in the henhouse analogy of yours. > I understand we've got good intentions here, but there is a mindset, > amongst certain pathologists, about cheap labor. Despite pay increases > in recent years (and I'm grateful) Histotechs overall, are the lowest > paid laboratorians. Increased educational requirements (which I believe > in) still have not eradicated this mindset. Please understand, I am not > speaking about all pathologists, but there are enough to validate the > analogy. > Now Barry, I think your educational background is great and I suspect > you're a better man because of it. It seems to me in this day and age > that it would be near impossible to pull off. We've taken incredible > leaps in technology just for Histology alone. I would be wary of an MLT > or MT today that thought they could come into our Histology lab and > perform at the level I expect (that whole dexterity thing again). And > frankly, I think it would be extremely difficult to go into the General > Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any > other sub-discipline and perform at an acceptable level. Now do I think > folks should have an understanding and appreciation for these other > disciplines? Absolutely. And maybe that needs to be incorporated in > Histology training at an academic level. But doing the work is another > thing entirely. > Anyway, I don't know if my opinion will count for much, but there you > have it. It would be nice to see some changes and I think reinstating > the practical is worth considering. I understand that logistical > problems exist as well, along with some of the other subjective > variables that Joe Nocito mentioned. Maybe judges could be sent > regional sites or something. Anyway it's food for thought. > Thanks for letting me ramble. > > Thomas Jasper HT (ASCP) BAS > AP Supervisor > SMDC - Duluth, MN > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Feb 4 10:42:31 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Feb 4 10:42:54 2007 Subject: [Histonet] EBER In-Reply-To: References: Message-ID: <45C5C6A70200007700004153@hcnwgwds01.hh.chs> Hi Patti: I have been very impressed with Vision BioSystems' EBER detection technology. We have been evaluating their EBER technology on their Bond Max instrument and the results have been spectacular. There are only two reagents that you need in addition to their IHC detection kit; the FITC-labeled EBER probe and an anti-FITC antibody. Everything except coverslipping is performed on the instrument. I can't believe how easy it is to do. I remember years ago when I had to "baby sit" the slides for the better part of two days. Now, I can get a result in approximately 4 hours completely automated. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Patti Loykasek 02/01/07 2:20 PM >>> I have an inquiry for anyone doing EBV in-situ hybridization. What type of probe are you using? How is your probe labeled? Are you using a kit & if so, what kit? We are looking to optimize our sensitivity. Currently we are using a digoxigen labeled EBV probe that we have made for us, detection is via an anti-dig link and then Envision+-HRP, followed by DAB. Thanks for the info. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Rcartun <@t> harthosp.org Sun Feb 4 10:58:43 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Feb 4 10:59:17 2007 Subject: [Histonet] ER control tissue In-Reply-To: References: Message-ID: <45C5CA73020000770000415C@hcnwgwds01.hh.chs> As we move forward with standardization in IHC testing (HER2, EGFr, ER, PR, CD20, CD117, etc.), we really need to start thinking about using control tissue (including TMAs) fixed and processed in your own laboratory. Although useful for proficiency testing, results obtained on someone else's tissue will not completely validate your testing. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 02/01/07 4:41 PM >>> Hello all - Does anyone know of a source to order ER controls for IHC -- other than Pantomics in California? Ideally I'm looking for an array that would contain a strong (3+), moderate (2+), and low/negative (1+) representation. Thanks for any help! Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From bhewlett <@t> cogeco.ca Sun Feb 4 11:33:40 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sun Feb 4 11:33:45 2007 Subject: [Histonet] ER control tissue References: <45C5CA73020000770000415C@hcnwgwds01.hh.chs> Message-ID: <001101c74882$9cf68b20$6500a8c0@mainbox> Hi Rich, I completely agree with your final sentence. However, complete in-house standardization of IHC testing (HER2, EGFr, ER, PR, CD20, CD117, etc.), absolutely has to include standardization of the in-house fixation and processing! Only once this has been achieved, and the controls in use are prepared in a truly similar manner to the test material, will we be able to have a standardized IHC test! Anything less means that we will continue to be shooting at a wildly moving target. Best regards, Bryan ----- Original Message ----- From: "Richard Cartun" To: ; Sent: Sunday, February 04, 2007 11:58 AM Subject: Re: [Histonet] ER control tissue As we move forward with standardization in IHC testing (HER2, EGFr, ER, PR, CD20, CD117, etc.), we really need to start thinking about using control tissue (including TMAs) fixed and processed in your own laboratory. Although useful for proficiency testing, results obtained on someone else's tissue will not completely validate your testing. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 02/01/07 4:41 PM >>> Hello all - Does anyone know of a source to order ER controls for IHC -- other than Pantomics in California? Ideally I'm looking for an array that would contain a strong (3+), moderate (2+), and low/negative (1+) representation. Thanks for any help! Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sun Feb 4 12:21:54 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Feb 4 12:24:12 2007 Subject: [Histonet] ER control tissue References: <45C5CA73020000770000415C@hcnwgwds01.hh.chs> <001101c74882$9cf68b20$6500a8c0@mainbox> Message-ID: <001401c74889$5b165c60$649eae18@yourxhtr8hvc4p> wouldn't using someone else's tissue kind of invalidate those trying to establish ASRs? Just wondering. Joe ----- Original Message ----- From: "Bryan Hewlett" To: "Richard Cartun" ; ; Sent: Sunday, February 04, 2007 11:33 AM Subject: Re: [Histonet] ER control tissue > Hi Rich, > > I completely agree with your final sentence. > However, complete in-house standardization of IHC testing (HER2, EGFr, ER, > PR, CD20, CD117, etc.), > absolutely has to include standardization of the in-house fixation and > processing! > Only once this has been achieved, and the controls in use are prepared in > a truly similar manner to the test material, > will we be able to have a standardized IHC test! > Anything less means that we will continue to be shooting at a wildly > moving target. > > Best regards, > Bryan > > ----- Original Message ----- > From: "Richard Cartun" > To: ; > Sent: Sunday, February 04, 2007 11:58 AM > Subject: Re: [Histonet] ER control tissue > > > As we move forward with standardization in IHC testing (HER2, EGFr, ER, > PR, CD20, CD117, etc.), we really need to start thinking about using > control tissue (including TMAs) fixed and processed in your own > laboratory. Although useful for proficiency testing, results obtained on > someone else's tissue will not completely validate your testing. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> 02/01/07 4:41 PM >>> > > > > > Hello all - > > Does anyone know of a source to order ER controls for IHC -- other > than > Pantomics in California? Ideally I'm looking for an array that would > contain a strong (3+), moderate (2+), and low/negative (1+) > representation. > > Thanks for any help! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schraz13 <@t> yahoo.com Sun Feb 4 12:37:38 2007 From: schraz13 <@t> yahoo.com (Scheherazade Humphrey) Date: Sun Feb 4 12:37:54 2007 Subject: [Histonet] Re: Blue Nevus Message-ID: <20070204183738.36451.qmail@web81013.mail.mud.yahoo.com> Hello Histoneters: I would like to know if anyone can help me with some specific information for my research paper/capstone on Blue Nevus. I have found information on doctor's doctor website and on emedicine, but the one thing that i am not finding is a case report in histological detail Preparation Assessment: (Fixing ? Staining) Evaluate the effectiveness of each histological tissue preparation step and its effect on a future patient diagnosis. Identify and describe histological tissue preparations unable to perform (and why) that would enhance the likelihood for a more accurate diagnosis. If anyone can help me out with my question, that would be greatly appreciated. Thanks in advance. Ms. S. Humphrey, Argosy U Student histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. AW: [Histonet] Sentinel node protocol? (Gudrun Lang) 2. RE: ASCP Exam Long opinion (patsy ruegg) 3. peptide-blocking (Carlos G. Perez-Garcia) 4. Re: Re: Nuclear Fast Red (Lynette Pavelich) 5. Re: ASCP Exam Long opinion (Joe Nocito) 6. Re: EBER (Richard Cartun) 7. Re: ER control tissue (Richard Cartun) 8. Re: ER control tissue (Bryan Hewlett) ---------------------------------------------------------------------- Message: 1 Date: Sat, 3 Feb 2007 19:38:56 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Sentinel node protocol? To: Message-ID: <001301c747c2$919b9ab0$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Last year we started a program for the breast sentinels with serial cuts in 50 ?m steps. The first and the last were HE stained, the others Pancytokeratin. The pathologists wanted to see, if there is any significant differenc in the results to our former protocol with 250?m steps. Now we turned again to the larger distance. We also do frozens on the half part of the sentinel if it is smaller than 5 mm, and on the middle 2mm-part if it is larger. We perform also one slide as rapid ck-pan. But for all good reasons to get the information of the frozen, we cannot be sure about the result until the last immuno-slide. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von MICHELLE SEAGLE Gesendet: Samstag, 03. Februar 2007 00:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sentinel node protocol? I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill. thanks Michelle Seagle Rutherford Hospital HT (ASCP) --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Sat, 3 Feb 2007 13:05:18 -0700 From: "patsy ruegg" Subject: RE: [Histonet] ASCP Exam Long opinion To: "'Joe Nocito'" , "'Rittman, Barry R'" , "'Edwards, R.E.'" , "'Jasper, Thomas G.'" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <200702032005.l13K5CC3070217@pro12.abac.com> Content-Type: text/plain; charset="US-ASCII" I think I am even more of a dinosaur than you Joe. I think all the automation is taking us farther and farther away from connecting with the patient which is after all why we do what we do. I came from a unique situation, early in my histology career I took a job at the U of Colorado with Dr. Matthew Block in the department of Hematology/Oncology. Dr. Block was a practicing hematologist/oncologist who also became a board certified Pathologist and set up his own histology lab because he did not like the service he got from the pathology/histology department. He literally treated the patient and did his own path processing and report. We would go with Dr. Block to the patient and assist with taking a bone marrow biopsy or lymph node biopsy, take it back to the lab, process it into GMA plastic in those days because Dr. Block wanted really thin tissue sections. He was a hematologist who looked at everything under oil immersion and insisted on impeccable cell morphology. We saw the faces of the people we were preparing tissue sections for. I remember helping mothers hold babies while bone marrow was taken. I think these experiences continue to remind me that there is a patient whose parts we are attending to. I know this experience is not common and certainly not practical anymore but I think the more and more we push buttons and don't look at our slides the farther we are removed from remembering that there are patients we need to do our best for. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, February 02, 2007 6:02 PM To: Rittman, Barry R; Edwards, R.E.; Jasper, Thomas G. Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Exam Long opinion I agree with you Barry. Techs need to know how the procedure is working to be able to troubleshoot when something happens. Is it the machine or the procedure? That's what I stressed when I was giving my intro to IHC lectures. Something intricate like immunos should be done manually until the tech is familiar with the procedure before they put slides on a machine. But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, I think I'll have a scotch. Enjoy Joe ----- Original Message ----- From: "Rittman, Barry R" To: "Edwards, R.E." ; "Jasper, Thomas G." Cc: Sent: Friday, February 02, 2007 8:59 AM Subject: RE: [Histonet] ASCP Exam Long opinion Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 3 Feb 2007 17:35:05 -0800 From: "Carlos G. Perez-Garcia" Subject: [Histonet] peptide-blocking To: histonet@lists.utsouthwestern.edu Message-ID: <7BDD827B-AF0D-49C4-9C52-46D43BF326CF@salk.edu> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed hi everybody i need to do a peptide-antibody blocking experiment to check the specificity of an antibody i made. i wonder if somebody has experience on that. i suppose that just adding 5-10 times more peptide than antibody in the solution will be enough right? does anybody have any protocol or suggestion? thanks in advance Carlos Carlos G. Perez-Garcia, Ph.D. ------------------------------ Message: 4 Date: Sat, 03 Feb 2007 22:41:39 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Re: Nuclear Fast Red To: , Message-ID: <45C50FA4020000EE00011055@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII I have used "Brazilliant" for a few years, and has given good nuclear staining. No complaints from the docs. Lynette >>> 02/02/07 11:28 PM >>> Beth Delescavage asks about sources for nuclear fast red. Nuclear fast red is a dye whose future is pretty uncertain. Anatech offers an alternative, "Brazilliant", brazilin in alum. Brazilin is a natural dye botanically and chemically related to hematoxylin, but it's red. Has anybody on the list tried this stuff? With what results? (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 4 Feb 2007 09:46:01 -0600 From: "Joe Nocito" Subject: Re: [Histonet] ASCP Exam Long opinion To: "patsy ruegg" , "'Rittman, Barry R'" , "'Edwards, R.E.'" , "'Jasper, Thomas G.'" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <006601c74873$941dac40$649eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Patsy, I am closer to you than you think. At my first lab after histology school at Keesler AFB, MS, I had to assist on bone marrows and worked in the blood drawing room when I had no pathologist. I saw how bone marrows were performed and it scared me to death when I had to embed and cut the blocks. I didn't not want to mess it up and then have the patient return for another procedure because of my mistake. There were many retirees in Miami that were getting cancer treatment at the base. Often, the regular patients would request me to draw their blood because I also joked with them and was able to get the blood on the first try. I remember one lady was trying to fix me up with her granddaughter, a total knockout. She had told her granddaughter so much about me that the granddaughter was coming done for the summer. Unfortunately, the grandmother succumbed to cancer and I never had the chance to meet her. The attending wanted an autopsy. I was the only tech so I had to assist on it. When the pathologist and I were about to start, I got teary eyed. The doc asked what was up, so I told him. He told me to go back to the lab and he would get someone else to put her back in the cooler when he was done, all I had to do was clean up. Number 1= you are right, we just don't have the patient interaction we used to. It is hard for tech to try in put a face with the block. Still, to this day, 3 years later, I remember those experiences. Experiences that students today may or may not experience. Number 2= some pathologists today would not have understood and would tell me to suck it up. Today it's more of a turn around time/insurance payment issue than ever. Joe ----- Original Message ----- From: "patsy ruegg" To: "'Joe Nocito'" ; "'Rittman, Barry R'" ; "'Edwards, R.E.'" ; "'Jasper, Thomas G.'" Cc: Sent: Saturday, February 03, 2007 2:05 PM Subject: RE: [Histonet] ASCP Exam Long opinion >I think I am even more of a dinosaur than you Joe. > I think all the automation is taking us farther and farther away from > connecting with the patient which is after all why we do what we do. > I came from a unique situation, early in my histology career I took a job > at > the U of Colorado with Dr. Matthew Block in the department of > Hematology/Oncology. Dr. Block was a practicing hematologist/oncologist > who > also became a board certified Pathologist and set up his own histology lab > because he did not like the service he got from the pathology/histology > department. He literally treated the patient and did his own path > processing and report. We would go with Dr. Block to the patient and > assist > with taking a bone marrow biopsy or lymph node biopsy, take it back to the > lab, process it into GMA plastic in those days because Dr. Block wanted > really thin tissue sections. He was a hematologist who looked at > everything > under oil immersion and insisted on impeccable cell morphology. We saw > the > faces of the people we were preparing tissue sections for. I remember > helping mothers hold babies while bone marrow was taken. I think these > experiences continue to remind me that there is a patient whose parts we > are > attending to. I know this experience is not common and certainly not > practical anymore but I think the more and more we push buttons and don't > look at our slides the farther we are removed from remembering that there > are patients we need to do our best for. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Friday, February 02, 2007 6:02 PM > To: Rittman, Barry R; Edwards, R.E.; Jasper, Thomas G. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP Exam Long opinion > > I agree with you Barry. Techs need to know how the procedure is working to > be able to troubleshoot when something happens. Is it the machine or the > procedure? That's what I stressed when I was giving my intro to IHC > lectures. Something intricate like immunos should be done manually until > the > > tech is familiar with the procedure before they put slides on a machine. > But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, > I > > think I'll have a scotch. Enjoy > > Joe > ----- Original Message ----- > From: "Rittman, Barry R" > To: "Edwards, R.E." ; "Jasper, Thomas G." > > Cc: > Sent: Friday, February 02, 2007 8:59 AM > Subject: RE: [Histonet] ASCP Exam Long opinion > > > Nothing is black and white nowadays and I think that the current === message truncated === Scheherazade H. --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From rjbuesa <@t> yahoo.com Sun Feb 4 12:38:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 4 12:38:17 2007 Subject: [Histonet] ER control tissue In-Reply-To: <001401c74889$5b165c60$649eae18@yourxhtr8hvc4p> Message-ID: <18778.48471.qm@web61221.mail.yahoo.com> I agree that there is no better control tissue for an inhouse IHC test than a positive control processed just like the tissue to be tested BUT if this approach is to be always used, where this "connects" with tests like the Her2Neu provided by Dako with a FAD approved protocol that uses as controls their cultured cells in pellets processed in a different way? Either the FDA will have to extend approval to our processing protocols and to used our own positive cases, or Dako has to explain their processing protocol to be followed by all labs (fat change of either!). Just a thoght! Ren? J. Joe Nocito wrote: wouldn't using someone else's tissue kind of invalidate those trying to establish ASRs? Just wondering. Joe ----- Original Message ----- From: "Bryan Hewlett" To: "Richard Cartun" ; ; Sent: Sunday, February 04, 2007 11:33 AM Subject: Re: [Histonet] ER control tissue > Hi Rich, > > I completely agree with your final sentence. > However, complete in-house standardization of IHC testing (HER2, EGFr, ER, > PR, CD20, CD117, etc.), > absolutely has to include standardization of the in-house fixation and > processing! > Only once this has been achieved, and the controls in use are prepared in > a truly similar manner to the test material, > will we be able to have a standardized IHC test! > Anything less means that we will continue to be shooting at a wildly > moving target. > > Best regards, > Bryan > > ----- Original Message ----- > From: "Richard Cartun" > To: ; > Sent: Sunday, February 04, 2007 11:58 AM > Subject: Re: [Histonet] ER control tissue > > > As we move forward with standardization in IHC testing (HER2, EGFr, ER, > PR, CD20, CD117, etc.), we really need to start thinking about using > control tissue (including TMAs) fixed and processed in your own > laboratory. Although useful for proficiency testing, results obtained on > someone else's tissue will not completely validate your testing. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> 02/01/07 4:41 PM >>> > > > > > Hello all - > > Does anyone know of a source to order ER controls for IHC -- other > than > Pantomics in California? Ideally I'm looking for an array that would > contain a strong (3+), moderate (2+), and low/negative (1+) > representation. > > Thanks for any help! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From bhewlett <@t> cogeco.ca Sun Feb 4 13:37:02 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sun Feb 4 13:37:11 2007 Subject: [Histonet] ER control tissue References: <18778.48471.qm@web61221.mail.yahoo.com> Message-ID: <002501c74893$db995540$6500a8c0@mainbox> Ren?, Dako DOES specify fixation time, i.e. 16-24 hours in NBF prior to processing! I suspect that not many clinical labs follow this ! If they did, then the target would be much more reproducibly demonstrated! Bryan ----- Original Message ----- From: Rene J Buesa To: Joe Nocito ; Bryan Hewlett ; Richard Cartun ; bamoe@gundluth.org ; histonet@lists.utsouthwestern.edu Sent: Sunday, February 04, 2007 1:38 PM Subject: Re: [Histonet] ER control tissue I agree that there is no better control tissue for an inhouse IHC test than a positive control processed just like the tissue to be tested BUT if this approach is to be always used, where this "connects" with tests like the Her2Neu provided by Dako with a FAD approved protocol that uses as controls their cultured cells in pellets processed in a different way? Either the FDA will have to extend approval to our processing protocols and to used our own positive cases, or Dako has to expla in their processing protocol to be followed by all labs (fat change of either!). Just a thoght! Ren? J. Joe Nocito wrote: wouldn't using someone else's tissue kind of invalidate those trying to establish ASRs? Just wondering. Joe ----- Original Message ----- From: "Bryan Hewlett" To: "Richard Cartun" ; ; Sent: Sunday, February 04, 2007 11:33 AM Subject: Re: [Histonet] ER control tissue > Hi Rich, > > I completely agree with your final sentence. > However, complete in-house standardization of IHC testing (HER2, EGFr, ER, > PR, CD20, CD117, etc.), > absolutely has to include standardization of the in-house fixation and < BR>> processing! > Only once this has been achieved, and the controls in use are prepared in > a truly similar manner to the test material, > will we be able to have a standardized IHC test! > Anything less means that we will continue to be shooting at a wildly > moving target. > > Best regards, > Bryan > > ----- Original Message ----- > From: "Richard Cartun" > To: ; > Sent: Sunday, February 04, 2007 11:58 AM > Subject: Re: [Histonet] ER control tissue > > > As we move forward with standardization in IHC testing (HER2, EGFr, ER, > PR, CD20, CD117, etc.), we really need to start thinking about using > control tissue (including TMAs) fixed and processed in your own > laboratory. Although useful for proficiency testing, results obtained on > someone else's t issue will not completely validate your testing. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> 02/01/07 4:41 PM >>> > > > > > Hello all - > > Does anyone know of a source to order ER controls for IHC -- other > than > Pantomics in California? Ideally I'm looking for an array that would > contain a strong (3+), moderate (2+), and low/negative (1+) > representation. > > Thanks for any help! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > _______________________________________________ > Histonet mailing list > Histonet@lists. utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo /histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From pruegg <@t> ihctech.net Sun Feb 4 13:54:02 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Feb 4 13:54:09 2007 Subject: [Histonet] EBER In-Reply-To: <45C5C6A70200007700004153@hcnwgwds01.hh.chs> Message-ID: <200702041953.l14JruX0095180@pro12.abac.com> Rich, This kind of use of automation after you have manually gone thru the steps before hand, and I know that you look at the slides and have certain expectations of performance, is the kind of automation that can help us move forward. When I express concerns about automation I am not talking about this, I am talking about instances were there is no practical training before using automation, and the users do not know what to expect or even look to see what they got. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Sunday, February 04, 2007 9:43 AM To: histonet; Patti Loykasek Subject: Re: [Histonet] EBER Hi Patti: I have been very impressed with Vision BioSystems' EBER detection technology. We have been evaluating their EBER technology on their Bond Max instrument and the results have been spectacular. There are only two reagents that you need in addition to their IHC detection kit; the FITC-labeled EBER probe and an anti-FITC antibody. Everything except coverslipping is performed on the instrument. I can't believe how easy it is to do. I remember years ago when I had to "baby sit" the slides for the better part of two days. Now, I can get a result in approximately 4 hours completely automated. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Patti Loykasek 02/01/07 2:20 PM >>> I have an inquiry for anyone doing EBV in-situ hybridization. What type of probe are you using? How is your probe labeled? Are you using a kit & if so, what kit? We are looking to optimize our sensitivity. Currently we are using a digoxigen labeled EBV probe that we have made for us, detection is via an anti-dig link and then Envision+-HRP, followed by DAB. Thanks for the info. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Feb 5 07:21:09 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Feb 5 07:21:19 2007 Subject: [Histonet] Art vs Science Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB453@nmdamailsvr.nmda.ad.nmsu.edu> I work for three vet pathologists in a State lab. My work has been described by one tech, as rotating med tech students rotate through our facility, as an "artist". I find myself being very annoyed at this description of what I do. Although there is beauty in the end product as far as colors go, what I do is a science. I've never worked as hard at anything as I did when I studied for my written/practical exam back in 1969. I sweat bullets! I earned that "HT ASCP" that I'm proud to add to my signature and calling me an "artist" is not complimentary as far as I'm concerned. And there's my two cents and Happy Monday to all! I'm just happy that Joe is back... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From lblazek <@t> digestivespecialists.com Mon Feb 5 07:35:22 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Feb 5 07:32:36 2007 Subject: [Histonet] Art vs Science References: <4D14F0FC9316DD41972D5F03C070908B0DB453@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684D01@bruexchange.digestivespecialists.com> I second that Sally! Both the artist and Joe parts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, February 05, 2007 8:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Art vs Science I work for three vet pathologists in a State lab. My work has been described by one tech, as rotating med tech students rotate through our facility, as an "artist". I find myself being very annoyed at this description of what I do. Although there is beauty in the end product as far as colors go, what I do is a science. I've never worked as hard at anything as I did when I studied for my written/practical exam back in 1969. I sweat bullets! I earned that "HT ASCP" that I'm proud to add to my signature and calling me an "artist" is not complimentary as far as I'm concerned. And there's my two cents and Happy Monday to all! I'm just happy that Joe is back... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Mon Feb 5 07:48:39 2007 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Mon Feb 5 07:48:53 2007 Subject: [Histonet] archives Message-ID: <9B4A77DF11463E4FB723D484214AE9BC025176D3@KALEXMB02.KaleidaHealth.org> Please tell me how I can access the histonet archives for information? Thank you. Peggy DiCarlo HT (ASCP) Orthopaedics Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Barry.R.Rittman <@t> uth.tmc.edu Mon Feb 5 07:54:38 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Feb 5 07:54:43 2007 Subject: [Histonet] Art vs Science Message-ID: I see nothing wrong in being called an artist. Artists if experts in their profession, use a great deal of science in their art. You are of course correct that what we do has a strong science footing but if we merely view what we do as science then something is lost. If you do not wonder at the organization of cells and tissues, the color during staining then you are missing one of the benefits of the job. If you fail to see such beauty in your everyday work then please look at some preparations of diatoms. These were arranged in a painstaking but artistic fashion and also provided a resolution check for objectives due to the uniformity of their foramina. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, February 05, 2007 7:35 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Art vs Science I second that Sally! Both the artist and Joe parts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, February 05, 2007 8:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Art vs Science I work for three vet pathologists in a State lab. My work has been described by one tech, as rotating med tech students rotate through our facility, as an "artist". I find myself being very annoyed at this description of what I do. Although there is beauty in the end product as far as colors go, what I do is a science. I've never worked as hard at anything as I did when I studied for my written/practical exam back in 1969. I sweat bullets! I earned that "HT ASCP" that I'm proud to add to my signature and calling me an "artist" is not complimentary as far as I'm concerned. And there's my two cents and Happy Monday to all! I'm just happy that Joe is back... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 5 08:00:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 5 08:00:34 2007 Subject: [Histonet] archives In-Reply-To: <9B4A77DF11463E4FB723D484214AE9BC025176D3@KALEXMB02.KaleidaHealth.org> Message-ID: <820276.95627.qm@web61213.mail.yahoo.com> Go to the main web-site and "click" on the main topic. That will lead you to a page were "Arcieves" are included. Ren? J. "DiCarlo, Margaret" wrote: Please tell me how I can access the histonet archives for information? Thank you. Peggy DiCarlo HT (ASCP) Orthopaedics Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From pmarcum <@t> vet.upenn.edu Mon Feb 5 08:44:34 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Feb 5 08:44:48 2007 Subject: [Histonet] Shirley Powell Message-ID: <6.2.5.6.2.20070205094324.01c6d288@vet.upenn.edu> I am trying to reach Shirley Powell in Georgia. Shirley Chould you please contact me ASAP? I need to ask you a question. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From pjfnefro <@t> duke.edu Mon Feb 5 09:12:04 2007 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Mon Feb 5 09:12:14 2007 Subject: [Histonet] Art vs Science In-Reply-To: References: Message-ID: <73994750-AE59-4A84-BB98-99C094BB73B2@duke.edu> I have to agree with Barry on this one. While the science of histology can be learned, albeit sometimes with great effort, and those who have expended that considerable effort to acquire proficiency at tissue preparation and staining can be justifiably proud of their accomplishment, being considered an artist is recognition by others that your work transcends proficiency. An artist is not only a competent technician or craftsman, but also possesses an innate ability to produce work that is aesthetically pleasing and has value beyond the diagnostic. I would hope all of us would aspire to produce work that is not only acceptable and useful, but actually beautiful. Personally, I can think of no greater compliment to a scientist than that his or her work is beautiful. -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center (although I went to UNC-CH) Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 Cell: (919)606-0163 Bet that's more than you ever wanted to know about me! On Feb 5, 2007, at 8:54 AM, Rittman, Barry R wrote: > I see nothing wrong in being called an artist. > Artists if experts in their profession, use a great deal of science in > their art. > You are of course correct that what we do has a strong science footing > but if we merely view what we do as science then something is lost. > If you do not wonder at the organization of cells and tissues, the > color > during staining then you are missing one of the benefits of the job. > If you fail to see such beauty in your everyday work then please > look at > some preparations of diatoms. > These were arranged in a painstaking but artistic fashion and also > provided a resolution check for objectives due to the uniformity of > their foramina. > Barry > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, > Sara > Sent: Monday, February 05, 2007 8:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Art vs Science > > I work for three vet pathologists in a State lab. My work has been > described by one tech, as rotating med tech students rotate through > our > facility, as an "artist". I find myself being very annoyed at this > description of what I do. Although there is beauty in the end product > as far as colors go, what I do is a science. I've never worked as > hard > at anything as I did when I studied for my written/practical exam back > in 1969. I sweat bullets! I earned that "HT ASCP" that I'm proud to > add to my signature and calling me an "artist" is not complimentary as > far as I'm concerned. And there's my two cents and Happy Monday to > all! > > > > I'm just happy that Joe is back... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 From HornHV <@t> archildrens.org Mon Feb 5 09:16:12 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Feb 5 09:16:36 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: <006601c74873$941dac40$649eae18@yourxhtr8hvc4p> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6FB8@EMAIL.archildrens.org> I too had patient contact many years ago when I worked at Children's hospital in Oklahoma City. We went to pick up renal and liver biopsies and got to see the procedure and the patient. I enjoyed the patient contact and it made me more aware of always doing my best with our specimens. I wish all techs could have this experience because it does "bring it all home" that a real patient is behind every sample. As a breast cancer survivor (over 6 years, stage III) I am very interested in the discussions on processing breast and node specimens. (our hospital does not get those specimens) All of the patient's treatment depends upon the pathology report. I can't stress enough the need to follow all of the guidelines for ER/PR and Her2 processing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Sunday, February 04, 2007 9:46 AM To: patsy ruegg; 'Rittman, Barry R'; 'Edwards, R.E.'; 'Jasper, Thomas G.' Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Exam Long opinion Patsy, I am closer to you than you think. At my first lab after histology school at Keesler AFB, MS, I had to assist on bone marrows and worked in the blood drawing room when I had no pathologist. I saw how bone marrows were performed and it scared me to death when I had to embed and cut the blocks. I didn't not want to mess it up and then have the patient return for another procedure because of my mistake. There were many retirees in Miami that were getting cancer treatment at the base. Often, the regular patients would request me to draw their blood because I also joked with them and was able to get the blood on the first try. I remember one lady was trying to fix me up with her granddaughter, a total knockout. She had told her granddaughter so much about me that the granddaughter was coming done for the summer. Unfortunately, the grandmother succumbed to cancer and I never had the chance to meet her. The attending wanted an autopsy. I was the only tech so I had to assist on it. When the pathologist and I were about to start, I got teary eyed. The doc asked what was up, so I told him. He told me to go back to the lab and he would get someone else to put her back in the cooler when he was done, all I had to do was clean up. Number 1= you are right, we just don't have the patient interaction we used to. It is hard for tech to try in put a face with the block. Still, to this day, 3 years later, I remember those experiences. Experiences that students today may or may not experience. Number 2= some pathologists today would not have understood and would tell me to suck it up. Today it's more of a turn around time/insurance payment issue than ever. Joe ----- Original Message ----- From: "patsy ruegg" To: "'Joe Nocito'" ; "'Rittman, Barry R'" ; "'Edwards, R.E.'" ; "'Jasper, Thomas G.'" Cc: Sent: Saturday, February 03, 2007 2:05 PM Subject: RE: [Histonet] ASCP Exam Long opinion >I think I am even more of a dinosaur than you Joe. > I think all the automation is taking us farther and farther away from > connecting with the patient which is after all why we do what we do. > I came from a unique situation, early in my histology career I took a job > at > the U of Colorado with Dr. Matthew Block in the department of > Hematology/Oncology. Dr. Block was a practicing hematologist/oncologist > who > also became a board certified Pathologist and set up his own histology lab > because he did not like the service he got from the pathology/histology > department. He literally treated the patient and did his own path > processing and report. We would go with Dr. Block to the patient and > assist > with taking a bone marrow biopsy or lymph node biopsy, take it back to the > lab, process it into GMA plastic in those days because Dr. Block wanted > really thin tissue sections. He was a hematologist who looked at > everything > under oil immersion and insisted on impeccable cell morphology. We saw > the > faces of the people we were preparing tissue sections for. I remember > helping mothers hold babies while bone marrow was taken. I think these > experiences continue to remind me that there is a patient whose parts we > are > attending to. I know this experience is not common and certainly not > practical anymore but I think the more and more we push buttons and don't > look at our slides the farther we are removed from remembering that there > are patients we need to do our best for. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Friday, February 02, 2007 6:02 PM > To: Rittman, Barry R; Edwards, R.E.; Jasper, Thomas G. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP Exam Long opinion > > I agree with you Barry. Techs need to know how the procedure is working to > be able to troubleshoot when something happens. Is it the machine or the > procedure? That's what I stressed when I was giving my intro to IHC > lectures. Something intricate like immunos should be done manually until > the > > tech is familiar with the procedure before they put slides on a machine. > But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, > I > > think I'll have a scotch. Enjoy > > Joe > ----- Original Message ----- > From: "Rittman, Barry R" > To: "Edwards, R.E." ; "Jasper, Thomas G." > > Cc: > Sent: Friday, February 02, 2007 8:59 AM > Subject: RE: [Histonet] ASCP Exam Long opinion > > > Nothing is black and white nowadays and I think that the current > discussion on Histonet is important in putting forward all points of > view.. > > I agree that automation is very important for producing consistent > results and in many ways these have relieved us of tedious, repetitive > tasks. > I also respect the supervisors who are often stuck between a rock and a > hard place. > > The problem I currently see is that in many cases these machines may be > operated in a robotic fashion. If the individual operating the machine > is knowledgeable about the process taking place be it histochemical, > immunochemical or special stains and can take appropriate steps if > problems arise then there should be no problem. > Unfortunately in today's market, not just for histology, the emphasis > appears to be on minimal qualifications to carry out a task. This may > work perfectly well in those cases where instructions are concise and > easily followed and solutions changed at specific intervals and times > adhered to. > However, I believe something is lost to the histotechs in such a > situation. > > After all I operate my car with minimal knowledge of the mechanics and > electronics. However were I to get paid to operate my car I would hope > that I had time and make the effort to try understand the processes > involved. I would consider this part of responsibilities and also a > bonus in enhancing my work experience. > My point is, are we going to "progress" into an era where we are just > button pushers? > This automation also releases individual so that many histotechs may > carry out tasks that never used to be their responsibility. > This appears to be a national trend. As an example, I am paid as a > faculty member here but my time spent includes memos, filing etc. a task > originally carried out by secretarial staff. It is not that I resent > doing such tasks but after all I am paid more than secretaries and the > "secretarial tasks" never appear anywhere in my yearly report of > activities. > > Perhaps we will end up with histotechs plus a second group of button > pushers? Perhaps secretaries will be reincarnated - probably not. > > I guess that I am from an era where work was varied and pleasurable > rather than one in which the bottom line was the aim, and where employee > and employees worked as a team. > Hoe that this makes sense, this has been a one coffee morning so far. > Barry > > > -----Original Message----- > From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] > Sent: Friday, February 02, 2007 8:09 AM > To: Jasper, Thomas G.; Rittman, Barry R > Cc: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP Exam Long opinion > > Laboratory automation surely is as much due to obtaining > reproducibility of results than saving on staff costs, as to date > at least, all machines need human minders, who earn their corn > when the machine malfunctions and for example they have to do a > batch of H@Es by hand. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, > Thomas G. > Sent: 01 February 2007 18:17 > To: Rittman, Barry R > Cc: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP Exam Long opinion > > Hey Barry, > > I appreciate your long opinion, at times life requires long opinions. I > agree with much of what you say and I will try to concisely explain how > I see things. I totally agree that elimination of the practical was a > bad idea. And I understand the arguments for getting rid of the > practical. Let's start with automated staining. Unfortunately most > labs in the clinical world of the US, Can., UK, Western Europe, S. > Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned > to this technology out of necessity. This is due to staff shortages and > for cost containment. The consistency and reproducibility are greatly > enhanced by reduction of variables (I know more science than art). But > there you are, so a lab's automated stainer produces a stain, candidates > submit. If this is the technology a candidate will likely use, that > should be taken into consideration. > I expect a candidate to cut their own sections. That would be subject > to evaluation as it always was. And I expect someone to know what an H > and E or any other stain is supposed to look like, automated or not. > With so much emphasis on academics they should know what the stain looks > like and why. Heck, with all this automation you could even expect a > basic level of understanding about the mechanics involved and make that > part of the written exam. > Perhaps the elimination of the practical was not necessary, but a > reassessment of the whole thing, or as you so cleverly stated "...what > is needed for the entire system is a good enema!" I agree with the > statements from others that they want to know that folks who are > certified can cut sections. It is more balanced and despite all of our > wonderful automation Histology is the one laboratory discipline that > still requires a deft hand and an artistic eye. I'm not aware of any > automated substitute for manual dexterity. > I also agree with this whole ASCP/fox in the henhouse analogy of yours. > I understand we've got good intentions here, but there is a mindset, > amongst certain pathologists, about cheap labor. Despite pay increases > in recent years (and I'm grateful) Histotechs overall, are the lowest > paid laboratorians. Increased educational requirements (which I believe > in) still have not eradicated this mindset. Please understand, I am not > speaking about all pathologists, but there are enough to validate the > analogy. > Now Barry, I think your educational background is great and I suspect > you're a better man because of it. It seems to me in this day and age > that it would be near impossible to pull off. We've taken incredible > leaps in technology just for Histology alone. I would be wary of an MLT > or MT today that thought they could come into our Histology lab and > perform at the level I expect (that whole dexterity thing again). And > frankly, I think it would be extremely difficult to go into the General > Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any > other sub-discipline and perform at an acceptable level. Now do I think > folks should have an understanding and appreciation for these other > disciplines? Absolutely. And maybe that needs to be incorporated in > Histology training at an academic level. But doing the work is another > thing entirely. > Anyway, I don't know if my opinion will count for much, but there you > have it. It would be nice to see some changes and I think reinstating > the practical is worth considering. I understand that logistical > problems exist as well, along with some of the other subjective > variables that Joe Nocito mentioned. Maybe judges could be sent > regional sites or something. Anyway it's food for thought. > Thanks for letting me ramble. > > Thomas Jasper HT (ASCP) BAS > AP Supervisor > SMDC - Duluth, MN > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From slappycraw <@t> yahoo.com Mon Feb 5 09:35:28 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Feb 5 09:35:36 2007 Subject: [Histonet] Art vs Science In-Reply-To: <73994750-AE59-4A84-BB98-99C094BB73B2@duke.edu> Message-ID: <255476.77105.qm@web53613.mail.yahoo.com> I have heard the work describe as artistic before although I've never considered myself an artist while in the lab even though I've been tempted many times to photograph a slide and sell it on e-bay. I made my own X-mas cards over the holidays from scratch and got in touch with my artistic and creative side. What we do is somewhat an artform because it is creative but I don't think the line between art and science is as close as some may think. On the other note, I went into Histology specifically because there was no patient contact but that doesn't mean I'm not aware of the importance behind each and every specimen I handle. I've always treated every specimen as if it were my own and my life depended on it. Pat Flannery wrote: I have to agree with Barry on this one. While the science of histology can be learned, albeit sometimes with great effort, and those who have expended that considerable effort to acquire proficiency at tissue preparation and staining can be justifiably proud of their accomplishment, being considered an artist is recognition by others that your work transcends proficiency. An artist is not only a competent technician or craftsman, but also possesses an innate ability to produce work that is aesthetically pleasing and has value beyond the diagnostic. I would hope all of us would aspire to produce work that is not only acceptable and useful, but actually beautiful. Personally, I can think of no greater compliment to a scientist than that his or her work is beautiful. -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center (although I went to UNC-CH) Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 Cell: (919)606-0163 Bet that's more than you ever wanted to know about me! On Feb 5, 2007, at 8:54 AM, Rittman, Barry R wrote: > I see nothing wrong in being called an artist. > Artists if experts in their profession, use a great deal of science in > their art. > You are of course correct that what we do has a strong science footing > but if we merely view what we do as science then something is lost. > If you do not wonder at the organization of cells and tissues, the > color > during staining then you are missing one of the benefits of the job. > If you fail to see such beauty in your everyday work then please > look at > some preparations of diatoms. > These were arranged in a painstaking but artistic fashion and also > provided a resolution check for objectives due to the uniformity of > their foramina. > Barry > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, > Sara > Sent: Monday, February 05, 2007 8:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Art vs Science > > I work for three vet pathologists in a State lab. My work has been > described by one tech, as rotating med tech students rotate through > our > facility, as an "artist". I find myself being very annoyed at this > description of what I do. Although there is beauty in the end product > as far as colors go, what I do is a science. I've never worked as > hard > at anything as I did when I studied for my written/practical exam back > in 1969. I sweat bullets! I earned that "HT ASCP" that I'm proud to > add to my signature and calling me an "artist" is not complimentary as > far as I'm concerned. And there's my two cents and Happy Monday to > all! > > > > I'm just happy that Joe is back... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From rbruggeman <@t> psu.edu Mon Feb 5 09:58:39 2007 From: rbruggeman <@t> psu.edu (Richard Bruggeman) Date: Mon Feb 5 09:59:14 2007 Subject: [Histonet] Dako Reagent Vials Message-ID: <20070205T105839Z_48B8000B0000@psu.edu> If you are like me and are tired of dealing with Dako, then you will appreciate this notice. Dako reagent vials (the round 15mL vials with caps that are used on the Autostainer) can be purchased directly from Labvision (the manufacturer of the Dako Autostainer.) This solves the headache of having to wait on hold with Dako customer service and the inevitable back orders that will follow. I have repeatedly had reagent vials placed on back order with no clear or accurate indication as to when these items would actually arrive. NO MORE! I will now order all of my reagent vials directly from Labvision. In addition to having the vials in stock, the entire order process took only a bit over five minutes to complete, the vials will be FedExed to me, and on top of all that the exact same item cost 31% less from Labvision. How does Dako ever expect to compete? It's only a matter of time until customers are fed up with Dako's mistakes and will begin to look elsewhere for suppliers who can deliver. Trey Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 From TJJ <@t> Stowers-Institute.org Mon Feb 5 10:09:35 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Feb 5 10:10:11 2007 Subject: [Histonet] RE: Art vs. Science Message-ID: Pat wrote: >> I have to agree with Barry on this one. While the science of histology can be learned, albeit sometimes with great effort, and those who have expended that considerable effort to acquire proficiency at tissue preparation and staining can be justifiably proud of their accomplishment, being considered an artist is recognition by others that your work transcends proficiency. An artist is not only a competent technician or craftsman, but also possesses an innate ability to produce work that is aesthetically pleasing and has value beyond the diagnostic. I would hope all of us would aspire to produce work that is not only acceptable and useful, but actually beautiful. Personally, I can think of no greater compliment to a scientist than that his or her work is beautiful.<< Bravo this! And I love your sig as well. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Luis.Chiriboga <@t> med.nyu.edu Mon Feb 5 10:17:41 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Mon Feb 5 10:11:46 2007 Subject: [Histonet] Art vs Science In-Reply-To: Message-ID: Barry, I agree....everyone should appreciate the beauty and complexity in what we do. However,(here it comes) I think one of the issues is the perception that other scientist have of this type of work and of us as scientist. To quote Kevin Roth in his recent editorial "Labeling what we do as "art" absolves those not willing to approach molecular morphology as a scientific discipline, because after all, its art not science" I recently posted the citation to this article on the histonet. Its well worth the read. If you don't have access please let me know and I'll forward. Best Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Monday, February 05, 2007 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Art vs Science I see nothing wrong in being called an artist. Artists if experts in their profession, use a great deal of science in their art. You are of course correct that what we do has a strong science footing but if we merely view what we do as science then something is lost. If you do not wonder at the organization of cells and tissues, the color during staining then you are missing one of the benefits of the job. If you fail to see such beauty in your everyday work then please look at some preparations of diatoms. These were arranged in a painstaking but artistic fashion and also provided a resolution check for objectives due to the uniformity of their foramina. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, February 05, 2007 7:35 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Art vs Science I second that Sally! Both the artist and Joe parts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, February 05, 2007 8:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Art vs Science I work for three vet pathologists in a State lab. My work has been described by one tech, as rotating med tech students rotate through our facility, as an "artist". I find myself being very annoyed at this description of what I do. Although there is beauty in the end product as far as colors go, what I do is a science. I've never worked as hard at anything as I did when I studied for my written/practical exam back in 1969. I sweat bullets! I earned that "HT ASCP" that I'm proud to add to my signature and calling me an "artist" is not complimentary as far as I'm concerned. And there's my two cents and Happy Monday to all! I'm just happy that Joe is back... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Mon Feb 5 10:42:15 2007 From: bill501 <@t> mindspring.com (Bill) Date: Mon Feb 5 10:42:30 2007 Subject: [Histonet] Art vs Science In-Reply-To: <73994750-AE59-4A84-BB98-99C094BB73B2@duke.edu> References: <73994750-AE59-4A84-BB98-99C094BB73B2@duke.edu> Message-ID: At 10:12 AM -0500 2/5/07, Pat Flannery wrote: >Personally, I can think of no greater compliment to a scientist than >that his or her work is beautiful. Whenever I have made that comment, I have meant it as a compliment. I would certainly rather be considered an artist than a scientist, better both. But, 'art' implies creativity, and while I encourage creativity in my technicians and in myself, the #$%@ bureaucraps and regulators do everything in their power to stamp it out. They are the priests of mediocrity. Of course all of this is meaningless unless we understand 'art' and 'science' in the same way as individuals. -- ______________ Bill Blank, MD Heartland Lab From Jason.Wiese <@t> va.gov Mon Feb 5 10:54:35 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Mon Feb 5 10:55:46 2007 Subject: [Histonet] Art vs Science In-Reply-To: References: <73994750-AE59-4A84-BB98-99C094BB73B2@duke.edu> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E127B3@VHAV20MSGA3.v20.med.va.gov> I agree... all it takes to be a great scientist is a sharp mind and an analytical personality. To be an artist requires a passion for what you do. I don't think anyone was slamming you by calling you an artist. Rather, it was obvious to them, you take pride in your work. We are artists in the sense we become more skilled with time. We are artists in the sense not everyone can do what we do to the same degree of excellence. My 2 cents... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, February 05, 2007 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Art vs Science At 10:12 AM -0500 2/5/07, Pat Flannery wrote: >Personally, I can think of no greater compliment to a scientist than >that his or her work is beautiful. Whenever I have made that comment, I have meant it as a compliment. I would certainly rather be considered an artist than a scientist, better both. But, 'art' implies creativity, and while I encourage creativity in my technicians and in myself, the #$%@ bureaucraps and regulators do everything in their power to stamp it out. They are the priests of mediocrity. Of course all of this is meaningless unless we understand 'art' and 'science' in the same way as individuals. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 5 11:07:12 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 5 11:07:21 2007 Subject: [Histonet] The art of histology! Message-ID: <94092.43031.qm@web61219.mail.yahoo.com> Dear fellow histonetters: In 2003 I published a paper in the JOH titled; "Histochemistry: a case of unappreciated beauty?" where I deal with this issue and how our work is in all reality an art (more than medicine, that is also ofted described as the "art of medicine"). Calling an artist as such, is in no way detrimental (ask Rembrandt or Rubens if you could!) Those of you who would like a copy, please let me know. Ren? J. --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From alaskagirl1950 <@t> yahoo.com Mon Feb 5 11:02:17 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Mon Feb 5 11:15:07 2007 Subject: [Histonet] Art vs Science In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E127B3@VHAV20MSGA3.v20.med.va.gov> Message-ID: <20070205170217.79297.qmail@web52515.mail.yahoo.com> Very well said! Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail beta. http://new.mail.yahoo.com From sweaver <@t> bbpllab.com Mon Feb 5 11:11:28 2007 From: sweaver <@t> bbpllab.com (Steve Weaver) Date: Mon Feb 5 11:30:54 2007 Subject: [Histonet] FW: Clinic Message-ID: <813FB33DA405334F947F8BFC6EBD0B2A8490CA@bbplsrv1.bbpl> Adrian, this is Scott's email for the clinic. It may be full but you may want to try....also, we play the Orangemen back-to-back. Rising Stars-Coleman play the same team back-to-back, same dates as us, along with two other teams at 2pm. I think the other Rising Stars are boys...and I don't think we are ready for them. There is a way for the schedule to play out in two Sundays where no one plays the same team twice. Hopefully, this makes sense. If not, give me a call tonight. Thanks. Steve. Oh, our bench has not scored a point since Napheesa left (opposing team has scored more on themselves (2 pts in Fulton) than our bench has scored). I'm taking Cheyanna to the Arc Friday night for a shoot-around/fun. Anyone is welcome to attend. Steve -----Original Message----- From: Nielson, Scott [mailto:nielsons@missouri.edu] Sent: Monday, February 05, 2007 8:32 AM To: Steve Weaver Subject: RE: Clinic No problem Steve. I'm glad your daughter is practicing up on her skills. The February clinic is on Wednesday, February 21st from 6:30-7:30. Its prior to the 8:00 Mizzou Women's Basketball game against Kansas State that evening. I'll be in contact about a week prior with all the additional details you'll need. Thanks. Scott Nielson Assistant Marketing Director University of Missouri Department of Athletics Mizzou Arena 1 Champions Drive, Suite 200 Columbia, MO 65211 573-882-0362 (phone) 573-884-8885 (fax) nielsons@missouri.edu _____ From: Steve Weaver [mailto:sweaver@bbpllab.com] Sent: Saturday, February 03, 2007 6:10 PM To: Nielson, Scott Subject: Clinic Scott, I've misplaced the email you sent me for the February Women's Basketball Clinic for my daughter. What's the date again? (Sorry). She really enjoyed the one in January and has been dribbling between her legs since. Many thanks to you and Mizzou Athletics! Steve Weaver. From TownsendD <@t> childrensdayton.org Mon Feb 5 11:31:23 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Mon Feb 5 11:32:04 2007 Subject: [Histonet] Art vs Science Message-ID: And we are artists in the sense that we can come up with creative solutions when something breaks or falls appart. Think of Leonardo Da Vinci, artist, scientist and genius. My own 2 cents Dolores >>> "Wiese, Jason VHAROS" 2/5/2007 11:54 AM >>> I agree... all it takes to be a great scientist is a sharp mind and an analytical personality. To be an artist requires a passion for what you do. I don't think anyone was slamming you by calling you an artist. Rather, it was obvious to them, you take pride in your work. We are artists in the sense we become more skilled with time. We are artists in the sense not everyone can do what we do to the same degree of excellence. My 2 cents... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, February 05, 2007 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Art vs Science At 10:12 AM -0500 2/5/07, Pat Flannery wrote: >Personally, I can think of no greater compliment to a scientist than >that his or her work is beautiful. Whenever I have made that comment, I have meant it as a compliment. I would certainly rather be considered an artist than a scientist, better both. But, 'art' implies creativity, and while I encourage creativity in my technicians and in myself, the #$%@ bureaucraps and regulators do everything in their power to stamp it out. They are the priests of mediocrity. Of course all of this is meaningless unless we understand 'art' and 'science' in the same way as individuals. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Mon Feb 5 12:08:54 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Feb 5 12:08:58 2007 Subject: [Histonet] Immuno Qc Message-ID: <41E16A15CE78374EA45B57E0F94339B802058760@ORLEV01.hca.corpad.net> When testing Control QC- do you test each antibody for general controls...... Skins (ae1/ae3,cam 5.2) tonsils (cd's,lca,vim) or do you just test AE!/AE3 for skin and LCA for the tonsils for control positivity? When we receive new antibody we perform a test to see that the antibody worked........ I hope I'm making sense.....it was an early morning! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From ROrr <@t> enh.org Mon Feb 5 12:16:19 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Feb 5 12:16:28 2007 Subject: [Histonet] Sentinel node procedure Message-ID: We use a method modified from Cochran (March AJSP 30(3); 419-420.2006 (letter) Our modification is as follows. 10 levels are cut from designated block. (We use an identifier on the block to highlight the special handling needed) The depth between each level varies with the thickness of the sections. I generally try to take a section at around 50-80 micron intervals. The first sections taken from the block should not resemble the last sections. (making sure you have gone "deep" enough) All sections are placed on "plus" slides H/E on levels 1, 3, 5 and 10 Level 2 one slide for S100 (keep an extra unstained) Level 4 and 6 for Pan Melanoma Cocktail- Biocare (keep an extra unstained) Levels 7, 8, 9 remain unstained in case there's a need for further study. I believe the Cochran method describes using HMB-45 at level 4 and Mart-1 at level 6 Hope this helps! Brrrrrrrrrr! Becky Becky Orr CLA, HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 ----- Message: 24 Date: Fri, 2 Feb 2007 15:44:36 -0800 (PST) From: MICHELLE SEAGLE Subject: [Histonet] Sentinel node protocol? To: histonet@lists.utsouthwestern.edu Message-ID: <351317.23495.qm@web51809.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I was interested in knowing what other hospital labs use for their sentinel lymph node protocols for breast cases and melanoma cases. I think our hospital's is a little over kill. thanks Michelle Seagle Rutherford Hospital HT (ASCP) From debbiekeith <@t> cox.net Mon Feb 5 12:17:35 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Feb 5 12:17:43 2007 Subject: [Histonet] leasing microwave histoprocessors Message-ID: <5.2.0.9.0.20070205111543.03043550@pop.central.cox.net> hi histonetters! i'm interested in leasing a milestone RHS processor. the rep is in a training seminar all day... and i am impatient. :) do any of you lease your RHS? what is the typical lease price-length? i'm trying to SELL this to a cheap Dr.... it's gonna be tough! debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.411 / Virus Database: 268.17.26/670 - Release Date: 2/5/2007 From cpgarcia <@t> salk.edu Mon Feb 5 12:21:46 2007 From: cpgarcia <@t> salk.edu (Carlos G. Perez-Garcia) Date: Mon Feb 5 12:21:54 2007 Subject: [Histonet] peptide-ab blocking Message-ID: <6242E215-E22E-4716-97F1-6948A3A50135@salk.edu> hi everybody i need to do a peptide-antibody blocking experiment to check the specificity of an antibody i made. i wonder if somebody has experience on that. i suppose that just adding 5-10 times more peptide than antibody in the solution will be enough right? does anybody have any protocol or suggestion? thanks in advance Carlos Carlos G. Perez-Garcia, Ph.D. Carlos G. Perez-Garcia, Ph.D. Molecular Neurobiology Lab (MNL-O) The Salk Institute 10010 North Torrey Pines Road 92037 La Jolla, CA USA cpgarcia@salk.edu Fax: 858 558 6207 From JMacDonald <@t> mtsac.edu Mon Feb 5 12:25:22 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 5 12:25:38 2007 Subject: [Histonet] ASCP Exam Long opinion In-Reply-To: <011701c7472e$f2b05aa0$649eae18@yourxhtr8hvc4p> Message-ID: Joe, In our program students are required to do all staining by hand. This includes H&E's, specials, and IHC staining. They are also required to coverslip by hand. Jennifer "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/02/2007 05:02 PM To "Rittman, Barry R" , "Edwards, R.E." , "Jasper, Thomas G." cc Histonet@lists.utsouthwestern.edu Subject Re: [Histonet] ASCP Exam Long opinion I agree with you Barry. Techs need to know how the procedure is working to be able to troubleshoot when something happens. Is it the machine or the procedure? That's what I stressed when I was giving my intro to IHC lectures. Something intricate like immunos should be done manually until the tech is familiar with the procedure before they put slides on a machine. But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, I think I'll have a scotch. Enjoy Joe ----- Original Message ----- From: "Rittman, Barry R" To: "Edwards, R.E." ; "Jasper, Thomas G." Cc: Sent: Friday, February 02, 2007 8:59 AM Subject: RE: [Histonet] ASCP Exam Long opinion Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Feb 5 12:35:31 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Feb 5 12:35:41 2007 Subject: [Histonet] UNCLE! Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB461@nmdamailsvr.nmda.ad.nmsu.edu> I have read with interest the responses to my Innocent Post about "art vs science" and I am a Changed Woman. My original thought was not that I did not appreciate being called an "artist", but simply that the remark was made without regard to the years of training and experience that got me to the point that I can be called such. There is not a single day that I do not look at my simple little H&Es, ready to go to the docs, and experience a surge of pride that I do beautiful work (humbly speaking, of course). My point was that I was being portrayed (there's that Art thing again...) as being able to produce such work without giving credence to the training and experience necessary to do so. I do not believe for a minute that the observer meant any harm at all! I am quite happy that my work is beautiful (and my bosses have used that term many a time - for which I am humbled but proud), but also that it gives them the wherewithal to use their expertise to solve a mystery. I am happy to have had so much response to this, giving me the opportunity to reevaluate the subject and perhaps see it from another perspective. So, although my queries are not always technically-based and requiring highly technical input, it is these small subjects that allow us to exchange views and see the world differently. I shall now shake off my apron and dust off my easel and do some special stains. And wonder at their beauty. Thank you - everyone. This is a unique and valuable resource, this Histonet. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From pwg1 <@t> cdc.gov Mon Feb 5 12:57:25 2007 From: pwg1 <@t> cdc.gov (Greer, Patricia (CDC/CCID/NCZVED)) Date: Mon Feb 5 12:58:42 2007 Subject: [Histonet] Dako Reagent Vials References: <20070205T105839Z_48B8000B0000@psu.edu> Message-ID: Not only that but you can buy them directly from the manufacturer, Sarstedt (catalog # 62-732-012) and they are much cheaper there. Pat Greer Infectious Disease Pathology Branch Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Bruggeman Sent: Monday, February 05, 2007 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Reagent Vials If you are like me and are tired of dealing with Dako, then you will appreciate this notice. Dako reagent vials (the round 15mL vials with caps that are used on the Autostainer) can be purchased directly from Labvision (the manufacturer of the Dako Autostainer.) This solves the headache of having to wait on hold with Dako customer service and the inevitable back orders that will follow. I have repeatedly had reagent vials placed on back order with no clear or accurate indication as to when these items would actually arrive. NO MORE! I will now order all of my reagent vials directly from Labvision. In addition to having the vials in stock, the entire order process took only a bit over five minutes to complete, the vials will be FedExed to me, and on top of all that the exact same item cost 31% less from Labvision. How does Dako ever expect to compete? It's only a matter of time until customers are fed up with Dako's mistakes and will begin to look elsewhere for suppliers who can deliver. Trey Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 From tim.morken <@t> thermofisher.com Mon Feb 5 13:05:26 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Feb 5 13:05:28 2007 Subject: [Histonet] UNCLE! In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB461@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB461@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sally, maybe the misconception is that an artist does not have training and experience but just makes it up. Actually art is an exploration just like science and good artists make the difficult look easy, just like an experienced, knowledgable technologist. There was a story a few years ago about an artist who figured out how acrylic polymerizes. Chemists had not been able to control the polymerization of acrylics to make large structures - it was always getting too hot (polymerizing too fast) and cracking or staying gooy. This artist was trying to make a large solid clear acrylic sculpture but had the same problems. He studied and experimented with acrylics for years and one day it all came together for him. He was able to cast large structures that were perfectly polymerized with no defects. He went on to make different things that way and finally met someone who had another use for it - submarines. No one had ever been able to make a large window for submersibles. With the artists method they were able to and submersibiles with large bubble windows were developed. (http://www.brucebeasley.com/home.htm, look under Bathysphere). It's all the same, only the medium is different. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, February 05, 2007 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] UNCLE! I have read with interest the responses to my Innocent Post about "art vs science" and I am a Changed Woman. My original thought was not that I did not appreciate being called an "artist", but simply that the remark was made without regard to the years of training and experience that got me to the point that I can be called such. There is not a single day that I do not look at my simple little H&Es, ready to go to the docs, and experience a surge of pride that I do beautiful work (humbly speaking, of course). My point was that I was being portrayed (there's that Art thing again...) as being able to produce such work without giving credence to the training and experience necessary to do so. I do not believe for a minute that the observer meant any harm at all! I am quite happy that my work is beautiful (and my bosses have used that term many a time - for which I am humbled but proud), but also that it gives them the wherewithal to use their expertise to solve a mystery. I am happy to have had so much response to this, giving me the opportunity to reevaluate the subject and perhaps see it from another perspective. So, although my queries are not always technically-based and requiring highly technical input, it is these small subjects that allow us to exchange views and see the world differently. I shall now shake off my apron and dust off my easel and do some special stains. And wonder at their beauty. Thank you - everyone. This is a unique and valuable resource, this Histonet. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Mon Feb 5 13:56:32 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Mon Feb 5 13:56:51 2007 Subject: [Histonet] Re: Art vs Science Message-ID: Worthwhile to clarify some words here. Until fairly recently there wasn't any "Art with a capital A" - paintings by some guy with a beret starving in a garret and poisoning himself with lead and chromium and maybe cutting an ear off every now and then. In olden times when people called something an "art", all they meant was that it was a skill someone had learned. The word needed is "craft". The distinction between "fine art" and "humble craft" is a recent one, and in my opinion one we'd all be better off without. Pathology, histotechnology, and in fact all of medicine are crafts, helped along by varying amounts of science. A well known aphorism of Hippocrates (the half-legendary Greek physician over two thousand years ago) is that "life is short and art is long" (in Latin: vita brevis, ars longa). In Hippocrates' original Greek the word "art" is techne (skill or craft), and that's what the Latin means also. Bob Richmond Samurai Pathologist Knoxville TN From NMargaryan <@t> childrensmemorial.org Mon Feb 5 14:01:54 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Feb 5 14:02:36 2007 Subject: [Histonet] RE: Art vs Science References: Message-ID: <63B8B599DE283148B92E83C78B32C15D045A7908@cmhexbe2.childrensmemorial.org> I have to agree with Patrick and Barry too. Yes, I am an artist when see my beautiful staining in the paper. It is my art, it is our art. If your work could be called as an art it is the best compliment I have ever had. Naira's art, as my colleges called my pictures in the papers. Have good one too, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL? 60614-4314 Message: 12 Date: Mon, 5 Feb 2007 10:12:04 -0500 From: Pat Flannery Subject: Re: [Histonet] Art vs Science To: histonet@lists.utsouthwestern.edu Message-ID: <73994750-AE59-4A84-BB98-99C094BB73B2@duke.edu> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed I have to agree with Barry on this one. While the science of histology can be learned, albeit sometimes with great effort, and those who have expended that considerable effort to acquire proficiency at tissue preparation and staining can be justifiably proud of their accomplishment, being considered an artist is recognition by others that your work transcends proficiency. An artist is not only a competent technician or craftsman, but also possesses an innate ability to produce work that is aesthetically pleasing and has value beyond the diagnostic. I would hope all of us would aspire to produce work that is not only acceptable and useful, but actually beautiful. Personally, I can think of no greater compliment to a scientist than that his or her work is beautiful. -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center (although I went to UNC-CH) Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 Cell: (919)606-0163 Bet that's more than you ever wanted to know about me! On Feb 5, 2007, at 8:54 AM, Rittman, Barry R wrote: > I see nothing wrong in being called an artist. > Artists if experts in their profession, use a great deal of science in > their art. > You are of course correct that what we do has a strong science footing > but if we merely view what we do as science then something is lost. > If you do not wonder at the organization of cells and tissues, the > color > during staining then you are missing one of the benefits of the job. > If you fail to see such beauty in your everyday work then please > look at > some preparations of diatoms. > These were arranged in a painstaking but artistic fashion and also > provided a resolution check for objectives due to the uniformity of > their foramina. > Barry From blord <@t> swmail.sw.org Mon Feb 5 14:07:25 2007 From: blord <@t> swmail.sw.org (Barbara Lord) Date: Mon Feb 5 14:07:50 2007 Subject: [Histonet] Artist Message-ID: Here's my two cents The pathologist I trained under back in 1969 said it very well. Anybody can cut sections, but it takes an artist to do it well. Barbara TX From GMartin <@t> marshallhospital.org Mon Feb 5 14:19:17 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Mon Feb 5 14:17:12 2007 Subject: [Histonet] Art vs. Message-ID: I agree with Bob ... I have been waiting for some to mention that most likely closer to a craft. I'm one of those folks that seem to have been thrust into cutting tissue. I come form a high level craft back ground (art restoration) and found it quite easy to produce slides. I believe that is because of an extensive craft back ground. Gary Martin California From PMonfils <@t> Lifespan.org Mon Feb 5 14:46:06 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Feb 5 14:46:15 2007 Subject: [Histonet] UNCLE! In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C1B@LSRIEXCH1.lsmaster.lifespan.org> Perhaps a little experience I had some years ago will help put this in perspective. One day I observed one of our new residents carefully looking over my tissue processor. He looked at the front, both sides, and underneath. Finally I went over and said "can I help you?". He said (believe it or not) "I was looking for where the blocks come out". He figured you put the cassettes in the machine and the machine then spit out finished paraffin blocks. I explained to him that we make the blocks manually, one by one, and also section them, stain the slides and coverslip them manually. To which he responded, "so there is really an art to this histology business". He was right on this point. An artist takes raw materials and with his/her own hands and mind, creates something that was not there before. Histology requires scientific knowledge, manual dexterity and many fine skills which can be perfected only by long hours of practice. Other areas of medical technology also require knowledge, but for the most part fewer fine skills. You don't really need much manual skill to drop a tube into a machine that analyses the contents and prints out the results. From Barry.R.Rittman <@t> uth.tmc.edu Mon Feb 5 14:55:24 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Feb 5 14:55:30 2007 Subject: [Histonet] UNCLE! Message-ID: That is an improvement over some who have come to the lab and seemed to have their head stuck in a dark part of their digestive tract. Hopefully Darwinism will take care of that. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Monday, February 05, 2007 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] UNCLE! Perhaps a little experience I had some years ago will help put this in perspective. One day I observed one of our new residents carefully looking over my tissue processor. He looked at the front, both sides, and underneath. Finally I went over and said "can I help you?". He said (believe it or not) "I was looking for where the blocks come out". He figured you put the cassettes in the machine and the machine then spit out finished paraffin blocks. I explained to him that we make the blocks manually, one by one, and also section them, stain the slides and coverslip them manually. To which he responded, "so there is really an art to this histology business". He was right on this point. An artist takes raw materials and with his/her own hands and mind, creates something that was not there before. Histology requires scientific knowledge, manual dexterity and many fine skills which can be perfected only by long hours of practice. Other areas of medical technology also require knowledge, but for the most part fewer fine skills. You don't really need much manual skill to drop a tube into a machine that analyses the contents and prints out the results. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMcCormick <@t> schosp.org Mon Feb 5 15:15:56 2007 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Mon Feb 5 15:16:07 2007 Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filter detected spam In-Reply-To: References: Message-ID: <913FAC2B773C19488E26AE6572180FA509EA9ED6@exch01.schosp.org> ALL histonet friends, Who are interested in an "artform" known as "Histotechnology". Rather than beg the point of Hippocrates or the comments of Lettered Professors.........I would go on record as saying I am the oldest among you who aspires to observe and admire the beauty that is hidden in the most minute of natures gifts. The beauty of humming bird feathers. The opalescence of a butterfly egg. A germ cell in mitosis. Polarized light refracted from a thin ground specimen. ALL of nature, as revealed throug the naked eye, or microscopic view, holds beauty and "art" in it's hand. And, when the "hand" prepares this gift for viewing it is indeed an artisans hand. Behold the Gomori trichrome with added aldehyde fuchsin to separate elastic fibers or beta cells of the pancreas. Tell me now.... is this not an artform given by the gifted hands of an artist?????? Beauty, indeed, is within the eyes of the beholder and I am pleased to have the gift of eyes. May I refer you to a web site that will "blow you away" My friend Klaus Kemp prepares slides that are each an art offering with a palate of natures gifts. The likes of these have not been seen since the late 19 century when time permitted a "micro-artform" to be the ultimate discovery and appreciation/expression of natures secrets. Do Take a Look and "hold your breath" It's amazing. www.diatoms.co.uk I will be interested in your response, J.B.McCormick, M.D. Histotechnologist, first. Pathologist of 60 years, second. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Monday, February 05, 2007 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filter detected spam Worthwhile to clarify some words here. Until fairly recently there wasn't any "Art with a capital A" - paintings by some guy with a beret starving in a garret and poisoning himself with lead and chromium and maybe cutting an ear off every now and then. In olden times when people called something an "art", all they meant was that it was a skill someone had learned. The word needed is "craft". The distinction between "fine art" and "humble craft" is a recent one, and in my opinion one we'd all be better off without. Pathology, histotechnology, and in fact all of medicine are crafts, helped along by varying amounts of science. A well known aphorism of Hippocrates (the half-legendary Greek physician over two thousand years ago) is that "life is short and art is long" (in Latin: vita brevis, ars longa). In Hippocrates' original Greek the word "art" is techne (skill or craft), and that's what the Latin means also. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From JMacDonald <@t> mtsac.edu Mon Feb 5 15:36:32 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 5 15:36:39 2007 Subject: [Histonet] California Society for Histotechnology Message-ID: The California Society for Histotechnology is seeking nominations for the Board of Directors. Positions include: President, Vice President, Treasurer, Secretary, and Nominations and Elections Chair. We will also be seeking people to chair some of the committees. Also really needed is an editor for the newsletter. If interested please contact: Jennifer MacDonald at jmacdonald@mtsac.edu Lynne Kertamus-Porter at kertamus@juno.com From jmahoney <@t> alegent.org Mon Feb 5 15:41:37 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Mon Feb 5 15:42:04 2007 Subject: [Histonet] Science Message-ID: <45C750310200003C00003F45@gwia.alegent.org> I have always thought of Histology as part art, part science. The more I read everyone's comments here I'm leaning toward Histotechnologists being scientists with exceptional manual dexterity. We are not really artists in the true sense of the word, but more like skilled craftsmen. In my mind this is just as valuable and should be as highly regarded as the science aspect. The issue here is first of all, being called "artists" or "craftsmen" is not as highly regarded in the medical community. Secondly, we can't just be artists, we also need to be scientists to be fully competent technologists. We will never be shown the respect or earn the pay if we don't embrace the science and have the education to back it up. This is becoming more and more important as time goes on. When we are fully automated, which we will be, the ones of us who do not know and understand the science will be obsolete, like the knife sharpeners and autotechnicons of our past. I know there are many people out there who are still sharpening knives, etc. but for the majority of the Histotechnologists, we see more and more automation as each year passes. Processing, staining, embedding and yes, even cutting, will become obsolete. We, as Histotechnologists will have to know how to trouble shoot problems, verify that the instrumentation is producing quality results, assure specimen integrity, many of the things med techs do today. We will have to know the science. I'm just as nostalgic about the past as the next guy, but I also see and embrace the inevitable and exciting changes that are about to happen in this wonderful field. We are growing by giant steps and it has only just begun. I hope I'm around long enough to see The field of Histotechnology finally come into it's own. Wow, how was that for a long reply. Jan Omaha From japoteete <@t> saintfrancis.com Mon Feb 5 15:42:41 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Mon Feb 5 15:44:57 2007 Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam Message-ID: Who says there is no art in science? Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCormick, James Sent: Monday, February 05, 2007 3:16 PM To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam ALL histonet friends, Who are interested in an "artform" known as "Histotechnology". Rather than beg the point of Hippocrates or the comments of Lettered Professors.........I would go on record as saying I am the oldest among you who aspires to observe and admire the beauty that is hidden in the most minute of natures gifts. The beauty of humming bird feathers. The opalescence of a butterfly egg. A germ cell in mitosis. Polarized light refracted from a thin ground specimen. ALL of nature, as revealed throug the naked eye, or microscopic view, holds beauty and "art" in it's hand. And, when the "hand" prepares this gift for viewing it is indeed an artisans hand. Behold the Gomori trichrome with added aldehyde fuchsin to separate elastic fibers or beta cells of the pancreas. Tell me now.... is this not an artform given by the gifted hands of an artist?????? Beauty, indeed, is within the eyes of the beholder and I am pleased to have the gift of eyes. May I refer you to a web site that will "blow you away" My friend Klaus Kemp prepares slides that are each an art offering with a palate of natures gifts. The likes of these have not been seen since the late 19 century when time permitted a "micro-artform" to be the ultimate discovery and appreciation/expression of natures secrets. Do Take a Look and "hold your breath" It's amazing. www.diatoms.co.uk I will be interested in your response, J.B.McCormick, M.D. Histotechnologist, first. Pathologist of 60 years, second. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Monday, February 05, 2007 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filter detected spam Worthwhile to clarify some words here. Until fairly recently there wasn't any "Art with a capital A" - paintings by some guy with a beret starving in a garret and poisoning himself with lead and chromium and maybe cutting an ear off every now and then. In olden times when people called something an "art", all they meant was that it was a skill someone had learned. The word needed is "craft". The distinction between "fine art" and "humble craft" is a recent one, and in my opinion one we'd all be better off without. Pathology, histotechnology, and in fact all of medicine are crafts, helped along by varying amounts of science. A well known aphorism of Hippocrates (the half-legendary Greek physician over two thousand years ago) is that "life is short and art is long" (in Latin: vita brevis, ars longa). In Hippocrates' original Greek the word "art" is techne (skill or craft), and that's what the Latin means also. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Mon Feb 5 15:56:35 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Feb 5 15:56:42 2007 Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam In-Reply-To: Message-ID: <538702.51108.qm@web53611.mail.yahoo.com> Once there was an artist standing in front of a car with the hood up staring at the engine. Along came a Histotech and he asked the artist what he was doing. The artist replied I need to add some oil to the engine but I don't have a funnel. The Histotech right away offered up by saying he had one in his trunk that he normally used for measuring out alcohol, ect. Well the funnel turned out to be too large so they waited for the tow driver/mechanic to arrive. Once he got there he pulled out a funnel the same size as the one they had already tried so of course they told him it was too large to which he just smiled and said OK as he put it in the hole. Turns out the artist and the Histotech were trying to put the funnel in the hole where the dipstick went, so yes we are very much like artists. "Poteete, Jacquie A." wrote: Who says there is no art in science? Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCormick, James Sent: Monday, February 05, 2007 3:16 PM To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam ALL histonet friends, Who are interested in an "artform" known as "Histotechnology". Rather than beg the point of Hippocrates or the comments of Lettered Professors.........I would go on record as saying I am the oldest among you who aspires to observe and admire the beauty that is hidden in the most minute of natures gifts. The beauty of humming bird feathers. The opalescence of a butterfly egg. A germ cell in mitosis. Polarized light refracted from a thin ground specimen. ALL of nature, as revealed throug the naked eye, or microscopic view, holds beauty and "art" in it's hand. And, when the "hand" prepares this gift for viewing it is indeed an artisans hand. Behold the Gomori trichrome with added aldehyde fuchsin to separate elastic fibers or beta cells of the pancreas. Tell me now.... is this not an artform given by the gifted hands of an artist?????? Beauty, indeed, is within the eyes of the beholder and I am pleased to have the gift of eyes. May I refer you to a web site that will "blow you away" My friend Klaus Kemp prepares slides that are each an art offering with a palate of natures gifts. The likes of these have not been seen since the late 19 century when time permitted a "micro-artform" to be the ultimate discovery and appreciation/expression of natures secrets. Do Take a Look and "hold your breath" It's amazing. www.diatoms.co.uk I will be interested in your response, J.B.McCormick, M.D. Histotechnologist, first. Pathologist of 60 years, second. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Monday, February 05, 2007 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filter detected spam Worthwhile to clarify some words here. Until fairly recently there wasn't any "Art with a capital A" - paintings by some guy with a beret starving in a garret and poisoning himself with lead and chromium and maybe cutting an ear off every now and then. In olden times when people called something an "art", all they meant was that it was a skill someone had learned. The word needed is "craft". The distinction between "fine art" and "humble craft" is a recent one, and in my opinion one we'd all be better off without. Pathology, histotechnology, and in fact all of medicine are crafts, helped along by varying amounts of science. A well known aphorism of Hippocrates (the half-legendary Greek physician over two thousand years ago) is that "life is short and art is long" (in Latin: vita brevis, ars longa). In Hippocrates' original Greek the word "art" is techne (skill or craft), and that's what the Latin means also. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From jmahoney <@t> alegent.org Mon Feb 5 16:24:53 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Mon Feb 5 16:25:13 2007 Subject: [Histonet] Mucicarmine Message-ID: <45C75A550200003C00003F58@gwia.alegent.org> Have you all read the latest edition of The Innovator from Anatech? I don't mean to show any vendor preferance but WOW, what a great article. Thanks Anatech, for the wonderful story and the wonderful information. Jan Omaha From laurie.reilly <@t> jcu.edu.au Mon Feb 5 17:56:30 2007 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Mon Feb 5 17:56:44 2007 Subject: [Histonet] Sectioning tendon Message-ID: <5.2.0.9.0.20070206095506.03306cd8@mail.jcu.edu.au> Can anyone help with tips to get good teaching sections of tendon. Thanks in advance, Laurie. Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 From rjbuesa <@t> yahoo.com Tue Feb 6 07:00:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 6 07:00:46 2007 Subject: [Histonet] Science In-Reply-To: <45C750310200003C00003F45@gwia.alegent.org> Message-ID: <437950.37792.qm@web61223.mail.yahoo.com> Automatic sectioning???? I do not foresee that happening, at least NOT until a microtome is equipment with the computerized software that allows: 1- locate the tissue in the wax; 2- decide "a priori" HOW DEEP to section into that tissue (what information could be provided to the software to decide that?) during trimming; and 3- decide which sections to discard FOR EVER and which to use to stain. Since all recognition software have to be "fed" with initial information to enable the recognition, the only way would be to "feed" the information at the moment of cassetting. Later, that automaton will have also to decide when and how much to cool the block to section among many other decisions to take! Those are only few of the decisions that the automaton would have to able to take. Again, I do not foresee that happening any time soon (and probably never). Not even from the economic point of view. A good "reliable" histo-artist (or artisan if you prefer) will always be more reliable and cheap than that monstruous and unlikely to be developed automaton. Just a thought! Ren? J. Janice Mahoney wrote: I have always thought of Histology as part art, part science. The more I read everyone's comments here I'm leaning toward Histotechnologists being scientists with exceptional manual dexterity. We are not really artists in the true sense of the word, but more like skilled craftsmen. In my mind this is just as valuable and should be as highly regarded as the science aspect. The issue here is first of all, being called "artists" or "craftsmen" is not as highly regarded in the medical community. Secondly, we can't just be artists, we also need to be scientists to be fully competent technologists. We will never be shown the respect or earn the pay if we don't embrace the science and have the education to back it up. This is becoming more and more important as time goes on. When we are fully automated, which we will be, the ones of us who do not know and understand the science will be obsolete, like the knife sharpeners and autotechnicons of our past. I know there are many people out there who are still sharpening knives, etc. but for the majority of the Histotechnologists, we see more and more automation as each year passes. Processing, staining, embedding and yes, even cutting, will become obsolete. We, as Histotechnologists will have to know how to trouble shoot problems, verify that the instrumentation is producing quality results, assure specimen integrity, many of the things med techs do today. We will have to know the science. I'm just as nostalgic about the past as the next guy, but I also see and embrace the inevitable and exciting changes that are about to happen in this wonderful field. We are growing by giant steps and it has only just begun. I hope I'm around long enough to see The field of Histotechnology finally come into it's own. Wow, how was that for a long reply. Jan Omaha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From Derek.Papalegis <@t> tufts.edu Tue Feb 6 07:34:13 2007 From: Derek.Papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Feb 6 07:34:24 2007 Subject: [Histonet] frozens on fat Message-ID: <20070206083413.qbe7ke8jk48skkk0@webmail.tufts.edu> I just got asked today if it is possible to cut frozens on brown fat in mice. In the past, in a clinical setting, I know when cutting lymph nodes, we never were able to get any of the fat with them on the slides. Does anyone know if this is possible to do? If so, what techniques are you using to get the frozen fat sections? If it is not possible, can anyone offer any alternatives that I can give to the investigator? Thanks, Derek From ree3 <@t> leicester.ac.uk Tue Feb 6 07:55:24 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Feb 6 07:55:36 2007 Subject: [Histonet] frozens on fat In-Reply-To: <20070206083413.qbe7ke8jk48skkk0@webmail.tufts.edu> References: <20070206083413.qbe7ke8jk48skkk0@webmail.tufts.edu> Message-ID: We have successfully cut cryostat sections of mouse brown fat, using standard methods, freezing down in dry ice, embedded in OCT, followed by Oil Red O, was not a problem.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: 06 February 2007 13:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozens on fat I just got asked today if it is possible to cut frozens on brown fat in mice. In the past, in a clinical setting, I know when cutting lymph nodes, we never were able to get any of the fat with them on the slides. Does anyone know if this is possible to do? If so, what techniques are you using to get the frozen fat sections? If it is not possible, can anyone offer any alternatives that I can give to the investigator? Thanks, Derek _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Tue Feb 6 09:28:00 2007 From: bill501 <@t> mindspring.com (Bill) Date: Tue Feb 6 09:28:13 2007 Subject: [Histonet] frozens on fat In-Reply-To: References: <20070206083413.qbe7ke8jk48skkk0@webmail.tufts.edu> Message-ID: At 1:55 PM +0000 2/6/07, Edwards, R.E. wrote: >We have successfully cut cryostat sections of mouse brown fat, >using standard methods, freezing down in dry ice, embedded in >OCT, followed by Oil Red O, was not a problem.... I think the secret here is "freezing down in dry ice" rather than using just the cryostat. Freezing in a slurry of isopentane in liquid nitrogen also works for hi fat tissues, though that may be overkill for adipose tissue. It works wonders for brain FS. -- ______________ Bill Blank, MD Heartland Lab From PMonfils <@t> Lifespan.org Tue Feb 6 09:48:09 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Feb 6 09:48:19 2007 Subject: [Histonet] frozens on fat In-Reply-To: <20070206083413.qbe7ke8jk48skkk0@webmail.tufts.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C1D@LSRIEXCH1.lsmaster.lifespan.org> You can usually get this kind of tissue cold enough to section by spraying it thoroughly with one of the refrigerant sprays that most biological supply companies sell. This will bring the tissue down to -50 C. Then take the section quickly, before the block has a chance to start warming up. If you use an anti-roll plate on your cryostat, spray that also. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis > Sent: Tuesday, February 6, 2007 5:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] frozens on fat > > I just got asked today if it is possible to cut frozens on brown fat in > mice. In the past, in a clinical setting, I know when cutting lymph > nodes, we never were able to get any of the fat with them on the > slides. Does anyone know if this is possible to do? If so, what > techniques are you using to get the frozen fat sections? If it is not > possible, can anyone offer any alternatives that I can give to the > investigator? > > Thanks, > > Derek > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From pruegg <@t> ihctech.net Tue Feb 6 10:00:50 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Feb 6 10:01:19 2007 Subject: [Histonet] Sectioning tendon In-Reply-To: <5.2.0.9.0.20070206095506.03306cd8@mail.jcu.edu.au> Message-ID: <002901c74a07$f9d8ccf0$6501a8c0@Patsy> Reilly, That is tuff, literally, I have gotten decent sections by embedding tendons in GMA in the past, but no so good in paraffin. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Reilly Sent: Monday, February 05, 2007 4:57 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Sectioning tendon Can anyone help with tips to get good teaching sections of tendon. Thanks in advance, Laurie. Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ladylaynah <@t> yahoo.com Tue Feb 6 10:10:56 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Tue Feb 6 10:11:05 2007 Subject: [Histonet] frozens on fat In-Reply-To: <20070206083413.qbe7ke8jk48skkk0@webmail.tufts.edu> Message-ID: <456313.84953.qm@web37007.mail.mud.yahoo.com> Derek, I do Moh's Histology (human tissue, not mouse) where we encounter large fatty specimens from time to time. We orient the tissue then immerse it for approx 10 seconds in Lq nitrogen. We are able to get some pretty nice sections of fat when it is really cold. However, our fat sections tend to be on the thick side. Our good fatty sections range from 20 - 30 microns thick. Also, this is not brown fat. I'm not certain how brown fat will cut, but I do know that the colder the speicmen the better it will cut. Just take great care not to get freeze artifact in the cells from over doing the freezing. good luck! Connie McManus, HT University of Utah Dermatology Dept Salt Lake City, UT --- Derek Papalegis wrote: > I just got asked today if it is possible to cut > frozens on brown fat in > mice. In the past, in a clinical setting, I know > when cutting lymph > nodes, we never were able to get any of the fat with > them on the > slides. Does anyone know if this is possible to do? > If so, what > techniques are you using to get the frozen fat > sections? If it is not > possible, can anyone offer any alternatives that I > can give to the > investigator? > > Thanks, > > Derek > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. http://tv.yahoo.com/collections/265 From JMcCormick <@t> schosp.org Tue Feb 6 10:17:21 2007 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Tue Feb 6 10:17:33 2007 Subject: [SPAM-HC] - RE: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam - Email found in subject In-Reply-To: References: Message-ID: <913FAC2B773C19488E26AE6572180FA509EAA0EE@exch01.schosp.org> ALL, And Webster says " Art is the power of performing certain actions esp. as acquired by experience,study,or observation." and I say " This may often produce Art Work......ie prepared microscope slides to observe the gifts of nature." JBMc -----Original Message----- From: Poteete, Jacquie A. [mailto:japoteete@saintfrancis.com] Sent: Monday, February 05, 2007 3:43 PM To: McCormick, James; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam - Email found in subject Who says there is no art in science? Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCormick, James Sent: Monday, February 05, 2007 3:16 PM To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filterdetected spam ALL histonet friends, Who are interested in an "artform" known as "Histotechnology". Rather than beg the point of Hippocrates or the comments of Lettered Professors.........I would go on record as saying I am the oldest among you who aspires to observe and admire the beauty that is hidden in the most minute of natures gifts. The beauty of humming bird feathers. The opalescence of a butterfly egg. A germ cell in mitosis. Polarized light refracted from a thin ground specimen. ALL of nature, as revealed throug the naked eye, or microscopic view, holds beauty and "art" in it's hand. And, when the "hand" prepares this gift for viewing it is indeed an artisans hand. Behold the Gomori trichrome with added aldehyde fuchsin to separate elastic fibers or beta cells of the pancreas. Tell me now.... is this not an artform given by the gifted hands of an artist?????? Beauty, indeed, is within the eyes of the beholder and I am pleased to have the gift of eyes. May I refer you to a web site that will "blow you away" My friend Klaus Kemp prepares slides that are each an art offering with a palate of natures gifts. The likes of these have not been seen since the late 19 century when time permitted a "micro-artform" to be the ultimate discovery and appreciation/expression of natures secrets. Do Take a Look and "hold your breath" It's amazing. www.diatoms.co.uk I will be interested in your response, J.B.McCormick, M.D. Histotechnologist, first. Pathologist of 60 years, second. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Monday, February 05, 2007 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-Bayesian] - [Histonet] Re: Art vs Science - Bayesian Filter detected spam Worthwhile to clarify some words here. Until fairly recently there wasn't any "Art with a capital A" - paintings by some guy with a beret starving in a garret and poisoning himself with lead and chromium and maybe cutting an ear off every now and then. In olden times when people called something an "art", all they meant was that it was a skill someone had learned. The word needed is "craft". The distinction between "fine art" and "humble craft" is a recent one, and in my opinion one we'd all be better off without. Pathology, histotechnology, and in fact all of medicine are crafts, helped along by varying amounts of science. A well known aphorism of Hippocrates (the half-legendary Greek physician over two thousand years ago) is that "life is short and art is long" (in Latin: vita brevis, ars longa). In Hippocrates' original Greek the word "art" is techne (skill or craft), and that's what the Latin means also. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From sonya.martin <@t> soton.ac.uk Tue Feb 6 10:45:46 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Feb 6 10:46:02 2007 Subject: [Histonet] Staining frozen sections without fixation Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34FD@ISS-CL-EX-V1.soton.ac.uk> Hi All, I'm trying to get an aPDCA-1 antibody to work on frozen mouse spleen sections. The antibody is normally used for FACS however the company says that it works for immunhistochemistry. They recommend staining before fixation. Usually I cut frozen sections, dry overnight (room temp) and fix in acetone (10min at room temp) before putting the sections in PBS and continuing with staining procedure. If I stain before fixation; Will the sections survive? When should I fix - after primary, secondary etc? What should I fix with? Any help greatly appreciated. Sonya From sonya.martin <@t> soton.ac.uk Tue Feb 6 10:48:59 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Feb 6 10:50:25 2007 Subject: [Histonet] 33D1 DC marker Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34FE@ISS-CL-EX-V1.soton.ac.uk> Hi All, Has anyone used 33D1 (mouse DC marker) antibody from BD Pharmingen on frozen sections? I've tried it with the secondary they recommend (mouse anti-rat IgG 2b) but I dont see anything. I tried it on isolated DC's growing on coverslips and got a very faint staining. Any comments? Sonya From AGrobe2555 <@t> aol.com Tue Feb 6 11:25:40 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Feb 6 11:26:00 2007 Subject: [Histonet] Re:33D1 DC Marker Message-ID: Sonya, Have you tried an amplification system for your secondary? If you gave a bit more detail on your staining protocol, it would be helpful for trouble-shooting...... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From koellingr <@t> comcast.net Tue Feb 6 11:57:58 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Feb 6 11:58:10 2007 Subject: [Histonet] 33D1 DC marker Message-ID: <020620071757.15673.45C8C1A600009DFC00003D3922007481849D09020704040A0105@comcast.net> Sonya, What were the frozen sections of tissue-wise? 33D1 is a fairly recent and I've used it to stain in the IHC configuration you tried but remember that that target is not at all well characterized. It certainly doesn't appear to be a structural or housekeeping protein expressed in abundance. 1) Look at flow diagrams of something like spleen stained 33D1 and that 1 log shift in "dendritic cells?" is a really minute portion of the population and flow people are looking at 5-10K gated events (cells). It is possible that depending on what tissue you section and how big it is, there just may be very few 33D1+ cells around to see. 2) Again, the jury is out on what 33D1 even is. Certainly it can be upregulated or downregulated (see articles on GM-CSF stimulation or IL-4 induction of down-regulation available in literature). 3) How were your coverslip DC's isolated? Certainly they cannot, after multiple sorts or purifications, be very similar to in-vivo DC's. 4) to be sure to get enough to see, I'd do in-vivo stimulation of the mouse. There is literature on how to do that. 5) there are other companies that sell 33D1, if you are convinced BD (that I think is great) 33D1 is not working. Ray unemployed and at home so no current lab affiliation to list -------------- Original message -------------- From: "Martin S." > Hi All, > > Has anyone used 33D1 (mouse DC marker) antibody from BD Pharmingen on > frozen sections? I've tried it with the secondary they recommend (mouse > anti-rat IgG 2b) but I dont see anything. > I tried it on isolated DC's growing on coverslips and got a very faint > staining. > > Any comments? > > Sonya > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Feb 6 12:02:36 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Feb 6 12:03:01 2007 Subject: [Histonet] Novolink Message-ID: I was wondering if anyone has tried Vector's IHC polymer detection "Novolink" and their opinion of it. We are testing various detections for a project. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From coleman_manufacturing <@t> yahoo.com Tue Feb 6 12:04:33 2007 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Tue Feb 6 12:04:44 2007 Subject: [Histonet] Microwave Processing Message-ID: <335273.41626.qm@web37614.mail.mud.yahoo.com> To all Milestone users: Coleman Manufacturing & Design supplies all glass beakers to all Tissue processing microwave units. Our price is almost half the cost of the OEM (Milestone, TBS, Richard-Allen, and many more); plus the beaker is the same. Email or contact our office if you are interested. We also make glass beakers to customer specifications Thanks alot, Justin Coleman Coleman Manufacturing & Design 1131 US 321 Business By-Pass Winnsboro, SC 29180 803.633.2124 Office 803.635.9401 Fax From JMitchell <@t> uwhealth.org Tue Feb 6 12:05:50 2007 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Tue Feb 6 12:06:02 2007 Subject: [Histonet] MHC-1 Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB103558239@uwhis-xchng4.uwhis.hosp.wisc.edu> Would appreciate any input on sources for MHC-1 antibody expression in muscle. Thanks much! Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital and Clinics Neuromuscular Laboratory Manager Madison, WI From Heidi.Miers <@t> NAU.EDU Tue Feb 6 12:41:05 2007 From: Heidi.Miers <@t> NAU.EDU (Heidi Miers) Date: Tue Feb 6 12:41:18 2007 Subject: [Histonet] Ovary fixation Message-ID: <45D44662@webmail.nau.edu> I section dog and cat ovaries on a regular basis and am unhappy with the quality of the follicles. The primordial follicles are found near the surface and when I look at them under the microscope they all look like they have vacuoles in the oocytes. Some ovaries come out looking OK. I am wondering if anyone has suggestions. Could it be fixation? We use 10% formalin for at least 3 days. How about processing? Could it be that the xylene substitute I use isn't clearing to allow proper paraffin infiltration? Also, for those who use a regressive H&E-what type of eosin and for how long to you let it stain? Help! Thanks, Heidi Research Specialist, Sr. Imaging and Histology Core Facility Northern Arizona University Heidi.Miers@nau.edu 928-523-9422 From TJJ <@t> Stowers-Institute.org Tue Feb 6 12:46:46 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Feb 6 12:47:11 2007 Subject: [Histonet] RE: Staining frozen sections without fixation Message-ID: Sonya, You wrote: >>I'm trying to get an aPDCA-1 antibody to work on frozen mouse spleen sections. The antibody is normally used for FACS however the company says that it works for immunhistochemistry. They recommend staining before fixation. Usually I cut frozen sections, dry overnight (room temp) and fix in acetone (10min at room temp) before putting the sections in PBS and continuing with staining procedure. If I stain before fixation; Will the sections survive? When should I fix - after primary, secondary etc? What should I fix with?<< Have you tried it using acetone fixed frozen sections yet? If so, did you get any staining? You might need to use the antibody at a higher concentration than what you are accustomed to using for IHC. Are you using conjugated primary antibody? What detection method are you using? If you are using a rat anti-mouse PDCA-1, be sure to use a mouse-adsorbed anti-rat secondary antibody for better species specificity. It might be interesting to try doing whole mount incubation of say a FITC-labeled antibody in mouse spleen, overnight at 4 degrees C, rinses in PBS or TBS, then brief formalin fixation followed by sucrose cryoprotection**, freezing, and then sectioning. Use an anti-FITC AP (not HRP, so you don't have to worry about quenching endogenous peroxidase) or anti-FITC Alexa 488 for fluorescent detection. You can also try doing the entire immuno procedure on whole mount prior to fixation. Alkaline phosphatase activity and some fluorescence is maintained after formalin fixation and cryosectioning. Remember to run a negative control spleen sample in parallel with non-immune serum for the primary antibody incubation. **I believe with the immersion of the tissue in the buffer for an extended period of time (overnight), if you do not fix and cryoprotect you will see lots of freezing artifact due to the buffers soaking up into the tissue. I've never done anything like this before, so all I can offer are suggestions and a hearty "good luck!". Hopefully Gayle Callis will chime in with some other suggestions. It wouldn't surprise me to hear she's done something like this successfully in the past. FWIW, I'm not usually very optimistic when trying an antibody optimized for FACS in IHC. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From FUNKM <@t> mercyhealth.com Tue Feb 6 13:10:30 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Feb 6 13:11:05 2007 Subject: [Histonet] CAP Breast fixation for Her/2 Message-ID: Good Day, We are working on our CAP checklist and with the new guideline for breast tissue fixation for her/2. What are your thoughts and how best to document the time the breast tissue went in to formalin ? When it comes from Xray they do list the time and also surgery. Are we missing something ? Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 From asmith <@t> mail.barry.edu Tue Feb 6 14:28:14 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Feb 6 14:28:24 2007 Subject: [Histonet] art & science Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4040@exchsrv01.barrynet.barry.edu> Art, like science, takes training. Professional artists usually have 4 to 6 years of training, either as a B.F.A. or M.F.A. or as an apprenticeship for roughly the same length of time. (My aunt, a professional artist, went via formal training. My daughter, a histotech, took the apprenticeship route.) Art hopes to move the viewer; science hopes to educate him. Frequently, art also educates: Esteban Murillo's smaller paintings are masterful studies of human psychology. David Hogarth's etchings often educate more than they move. Science also moves: Movat's pentachrome is done as often for its beauty as for the sake of gaining information. Scientists' overwhelming preference for Schroedinger's wave equation over Dirac's equally correct matrix mechanics is esthetic. The study of free-living protozoa is driven primarily by awe at sheer variety of living things. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From rjbuesa <@t> yahoo.com Tue Feb 6 14:38:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 6 14:38:11 2007 Subject: [Histonet] Ovary fixation In-Reply-To: <45D44662@webmail.nau.edu> Message-ID: <22779.20784.qm@web61214.mail.yahoo.com> Try infiltration with a mixture of ethanol (E) + isopropanol (I) + mineral oil (M) at: E:I:M (2:3:1) at 45?C for 1.50 hours followed by E:I:M (1:3:2) at 50?C for 1.25h Complete with 4 paraffin baths at 58?C of 0.75, 0.30, 0.30 and 0.50 h No xylene or substitutes are needed. More gentler and better infiltration is obtained. Ren? J. Heidi Miers wrote: I section dog and cat ovaries on a regular basis and am unhappy with the quality of the follicles. The primordial follicles are found near the surface and when I look at them under the microscope they all look like they have vacuoles in the oocytes. Some ovaries come out looking OK. I am wondering if anyone has suggestions. Could it be fixation? We use 10% formalin for at least 3 days. How about processing? Could it be that the xylene substitute I use isn't clearing to allow proper paraffin infiltration? Also, for those who use a regressive H&E-what type of eosin and for how long to you let it stain? Help! Thanks, Heidi Research Specialist, Sr. Imaging and Histology Core Facility Northern Arizona University Heidi.Miers@nau.edu 928-523-9422 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From AnthonyH <@t> chw.edu.au Tue Feb 6 16:37:36 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Feb 6 16:37:51 2007 Subject: [Histonet] Staining frozen sections without fixation Message-ID: Sonya, Air-drying frozen sections is a form of fixation. Acetone is a poor fixative. It probably has some action by dehydrating tissues. It may also aid in the removal of fat and other hydrophobic cellular components, thus aiding access of hydrophilic antibodies. I would not leave sections to air-dry so long, 30 mins would suffice and I would not bother fixing after the IPX procedure. Morphology will not change. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 > Bandaged Bear Day - Friday 30th March 2007 > For more information and to order merchandise visit www.bandagedbearday.com.au > > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Wednesday, 7 February 2007 3:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining frozen sections without fixation Hi All, I'm trying to get an aPDCA-1 antibody to work on frozen mouse spleen sections. The antibody is normally used for FACS however the company says that it works for immunhistochemistry. They recommend staining before fixation. Usually I cut frozen sections, dry overnight (room temp) and fix in acetone (10min at room temp) before putting the sections in PBS and continuing with staining procedure. If I stain before fixation; Will the sections survive? When should I fix - after primary, secondary etc? What should I fix with? Any help greatly appreciated. Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From laurie.colbert <@t> huntingtonhospital.com Tue Feb 6 17:09:37 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Feb 6 17:09:48 2007 Subject: [Histonet] Microwave Testing Message-ID: <57BE698966D5C54EAE8612E8941D76831998C1@EXCHANGE3.huntingtonhospital.com> I know this topic has been discussed before, but I can't find my info. What are others doing regarding the CAP questions regarding microwave leakage and temperature reproducibility? There is no one in my hospital that monitors microwaves - I will have to do it myself. Does anyone have info on ordering some kind of monitor? Do these questions apply only to those lab using microwaves for processing and/or staining? We will occasionally perform a special stain in the microwave (not regularly) - we normally just heat water, agar, etc. Laurie Colbert From gcallis <@t> montana.edu Tue Feb 6 17:29:42 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Feb 6 17:30:00 2007 Subject: [Histonet] immunostaining unfixed frozen sections- long email Message-ID: <6.0.0.22.1.20070206140921.01b11e08@gemini.msu.montana.edu> Dear Sonya and Teri, Teri, yes I have done this, but is tricky to keep section on the slide and was a bit tedious with the fear of losing the section. We prefer to do either acetone or acetone alcohol fixation of an overnight air dried frozen section, then and a standard immunofluorescence staining with primary and a secondary conjugated to a fluorophore. As for a direct FITC conjugated primary antibody, you may have problems if the FITC quenches itself. This happens with CD4-FITC antibodies (also CD8-FITC), The problem is caused by the FITC molecule in close proximity to other adjacent FITC molecules ( as my physical chemist hubby explained) the FITC quenches. This is NOT photobleaching, which is cause by exposure of fluorophores to room light and/or UV excitation light during microscopy. For unfixed frozen section staining, I worked with a purified CD4 antibody at a very high concentration 3 ug/ml (do a dilution panel), then after staining, fixed with formalin or paraformaldehyde and come back after fixation with a secondary antibody. You could try a direct FITC conjugated primary, then fix, rinse and mount a coverslip. If you get no staining, you may want to consider the quenching problem. Now for unfixed frozen section immunostaining particulars Frozen section mounted on Plus charge slide. This may be the time to try the Plus Gold slides which are touted to hold frozen sections better. Air dry the frozen section (overnight at RT should work). Draw lines across slide above and below the section so you can add 100 ul of any reagent, ImmEdge hydrophobic barrier pens from Vector are excellent, and it is easier to blot from a corner rather than a circles edge. CD4 antibody (not a FITC conjugate) was at 3 ug/ml concentration. Diluent for CD4 was 5 to 10% goat/2.5% mouse serum and is the normal serum block. You can try a normal serum block and in this case it would have been the 5 to 10% goat serum with 2.5% mouse, change the normal serum, we do not use super blocks for this protocol Primary incubations are done at 4C, and the buffer contained normal serum (0.1 to 0.2% matched to host of secondary or goat serum). We avoided detergent due to the delicate frozen section. If you want to do an overnight incubation or enzyme method after fixation, you need to add sodium azide, 0.1% to retard any bacterial growth. Azide also retards endogenous peroxidase. Protocol 1. Lay slides flat in humidity chamber 2. Add buffer with a plastic Pasteur pipette introduced from side of section rather than squirt on top of section - very slow flow. This will rinse away OCT, let stand approx 1 minute then pick up slide, drain it onto a towel, blot from corner. Work one slide at a time. 3. Add normal serum block in same manner, incubate at 4C for 20 min or so 4. Blot off normal serum block, blot from corner, don't rinse! 5. Add primary antibody and incubate for 30 to 60 minutes (you will have to do a time study to see what is best for your antibody) 6. Drain off antibody, add rinse buffer gently for a minute or so, drain off and repeat. Don't use coplin jar rinsing, this is too vigorous. 7. Fix in 2% NBF by immersing slides into fixative for 8 to 10 min. This is diluted NBF or you can make it up this way. If you plan to do HRP method after fixation, you could try a gentle say 0.03% H2O2 in buffer or do AP method to avoid this block. Do not use a methanol peroxidase block, opt for buffer/H2O2 instead. 8. After fixation, resume a normal immunostaining protocol by adding a secondary conjugated to a fluorophore or even enzyme or biotin, then proceed with Strepavidin-HRP, etc. The fellow who taught me this used Vector ABC kit. If you use this, I suggest using an F(ab')2 secondary and if we used a fluorophore conjugated secondary antibody it was F(ab')2 frag of IgG and adsorbed to mouse. You will have to adjust your antibody concentrations, maybe even the secondary antibody. Good luck Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 Sonya, You wrote: >>I'm trying to get an aPDCA-1 antibody to work on frozen mouse spleen sections. The antibody is normally used for FACS however the company says that it works for immunhistochemistry. They recommend staining before fixation. Usually I cut frozen sections, dry overnight (room temp) and fix in acetone (10min at room temp) before putting the sections in PBS and continuing with staining procedure. If I stain before fixation; Will the sections survive? When should I fix - after primary, secondary etc? What should I fix with?<< Have you tried it using acetone fixed frozen sections yet? If so, did you get any staining? You might need to use the antibody at a higher concentration than what you are accustomed to using for IHC. Are you using conjugated primary antibody? What detection method are you using? If you are using a rat anti-mouse PDCA-1, be sure to use a mouse-adsorbed anti-rat secondary antibody for better species specificity. It might be interesting to try doing whole mount incubation of say a FITC-labeled antibody in mouse spleen, overnight at 4 degrees C, rinses in PBS or TBS, then brief formalin fixation followed by sucrose cryoprotection**, freezing, and then sectioning. Use an anti-FITC AP (not HRP, so you don't have to worry about quenching endogenous peroxidase) or anti-FITC Alexa 488 for fluorescent detection. You can also try doing the entire immuno procedure on whole mount prior to fixation. Alkaline phosphatase activity and some fluorescence is maintained after formalin fixation and cryosectioning. Remember to run a negative control spleen sample in parallel with non-immune serum for the primary antibody incubation. **I believe with the immersion of the tissue in the buffer for an extended period of time (overnight), if you do not fix and cryoprotect you will see lots of freezing artifact due to the buffers soaking up into the tissue. I've never done anything like this before, so all I can offer are suggestions and a hearty "good luck!". Hopefully Gayle Callis will chime in with some other suggestions. It wouldn't surprise me to hear she's done something like this successfully in the past. FWIW, I'm not usually very optimistic when trying an antibody optimized for FACS in IHC. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------- Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From RSRICHMOND <@t> aol.com Wed Feb 7 01:08:53 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Feb 7 01:09:11 2007 Subject: [Histonet] Re: frozens on fat Message-ID: In 1970 I worked with a research histochemist, Bob Wyllie of blest memory, an Australian who I wish had published some of the things he knew. He took one of the old 1960's Damon-IEC black-box cryostats, cleaned all the lubricant out of it, and lubricated it (daily, admittedly an inconvenience) with a silicone lubricant, packed the cryostat with dry ice, and cut thin sections of straight fat. I may have the specs for the lubricant around here someplace - I tried to steal all his secrets that year - he was very helpful about that - Only English speaker I ever met who could cuss in Vietnamese. Bob Richmond Samurai Pathologist Knoxville TN From ian.montgomery <@t> bio.gla.ac.uk Wed Feb 7 02:59:10 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Feb 7 02:59:24 2007 Subject: Fw: [Histonet] Ovary fixation Message-ID: <003901c74a96$3b687600$4724d182@ibls.gla.ac.uk> Heidi, Ovaries, I normally fix using Bouin. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Heidi Miers" To: Sent: Tuesday, February 06, 2007 6:41 PM Subject: [Histonet] Ovary fixation > I section dog and cat ovaries on a regular basis and am unhappy with the > quality of the follicles. The primordial follicles are found near the surface > and when I look at them under the microscope they all look like they have > vacuoles in the oocytes. Some ovaries come out looking OK. I am wondering if > anyone has suggestions. Could it be fixation? We use 10% formalin for at least > 3 days. How about processing? Could it be that the xylene substitute I use > isn't clearing to allow proper paraffin infiltration? > Also, for those who use a regressive H&E-what type of eosin and for how long > to you let it stain? > Help! > Thanks, Heidi > > Research Specialist, Sr. > Imaging and Histology Core Facility > Northern Arizona University > Heidi.Miers@nau.edu > 928-523-9422 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sonya.martin <@t> soton.ac.uk Wed Feb 7 06:42:18 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Feb 7 06:42:38 2007 Subject: [Histonet] 33D1 DC marker- further info References: <020620071757.15673.45C8C1A600009DFC00003D3922007481849D09020704040A0105@comcast.net> Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3500@ISS-CL-EX-V1.soton.ac.uk> Hi All, Some further info; The sections were mouse spleen - we did some flow analysis with 33D1 of splenocytes but didnt see anything - hard to know if this is because there are just so few 33D1+ cells or if theres a problem with the antibody. There are a few papers out there with quite a lot of staining with 33D1 in mouse spleen - most recently in Science (Dudziak et al Vol 315 Jan 2007). For frozen sections I have tried 33D1 + mouse anti-rat IgG2b:Biotin with Streptavidin Alexa488 and also 33D1 + rb anti-rat:Biotin with tyramide amplification and Streptavidin Alexa488. The coverslip DC's were BMDC's - BM cells were harveted from mice and grown with GMCSF for 5 days before transfering cells to PLL coated coverslips. Cells were allowed to attach for 2hrs and were then fixed with 4% PFA, 10min. Basically we want to look at DC's in the spleen in conjuction with a number of other cell markers. We are using CD11c and Dec205 antibodies but wanted an additional DC marker. Thanks for all your suggestions/comments Sonya From DMSmith <@t> ghsnet.org Wed Feb 7 07:44:55 2007 From: DMSmith <@t> ghsnet.org (Donna Smith) Date: Wed Feb 7 07:41:31 2007 Subject: [Histonet] Question Message-ID: Does anyone know if there is this type of list serve for clinical laboratory (especially chemistry)? Thanks Donna M. Smith H.T. (ASCP) Pathology Manager Gwinnett Medical Center 678-442-4206 dmsmith@ghsnet.org This email communication, including any attached files, may contain material that is protected, proprietary,privileged, confidential, or otherwise legally exempt from disclosure. This communication is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient or the person responsible for delivering this communication to the intended recipient, you are prohibited from retaining, using, disseminating, forwarding, printing or copying this communication or taking any action based on the contents of this communication. If you have received this communication in error, please immediately notify the sender by replying to this email, and then delete the original message and attachments. From koellingr <@t> comcast.net Wed Feb 7 08:50:08 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Feb 7 08:50:20 2007 Subject: [Histonet] 33D1 DC marker- further info Message-ID: <020720071450.15110.45C9E7200003FBDE00003B0622070016419D09020704040A0105@comcast.net> Sonya, I guess my very greatest concern is from your first sentence. You did flow but "didn't see anything". I guess I would never even attempt IHC if I didn't see target verified by flow for an uncharacterized target. Mouse spleenocytes stained with 33D1 do give a one log shift and there are enough of them you can see them. This is not a shoulder off of the negative peak. I've seen numbers of target sites per cell estimated at 10,000 molecules per cell so this should be seen. Assuming you are not using a very strange strain of mouse since haplotype restriction has not been fully assessed. As far as the BMDC's plated to glass after GMCSF stimulation. Were these verified? In-vivo stimulations sometimes do fail with resultant lack of expansion of the targeted population. My cell culture experimental stimulations were never 100% successful. No ones are due to multiple reasons. After 5 days in GMCSF, were those cells flowed? I think originally you said the coverslip staining was "weak" and that shouldn't be. Check them by flow first. Bad GMCSF, wrong dilution calculation? Your stain setup looks good - it does and should work. The markers CD11c and CD205 are fine and MIDC-8 is another. But I guess my bottom line first and foremost is I wouldn't waste even another second doing IHC until I can stain 33D1 in flow and on those coverslips just to be sure my control standards are there and functioning. If you are flowing mouse splenocytes, can pick up a dendritic cell population by CD11c or 205, and then can't see any 33D1 at all - something is wrong long before IHC problems are there. Bad mouse, bad 33D1, bad Alexa 488, bad something but it is a lot easier to troubleshoot flow or coverslip staining of stimmed BMDC's and get that working than to troubleshoot IHC staining up front. Ray -------------- Original message -------------- From: "Martin S." Hi All, Some further info; The sections were mouse spleen - we did some flow analysis with 33D1 of splenocytes but didnt see anything - hard to know if this is because there are just so few 33D1+ cells or if theres a problem with the antibody. There are a few papers out there with quite a lot of staining with 33D1 in mouse spleen - most recently in Science (Dudziak et al Vol 315 Jan 2007). For frozen sections I have tried 33D1 + mouse anti-rat IgG2b:Biotin with Streptavidin Alexa488 and also 33D1 + rb anti-rat:Biotin with tyramide amplification and Streptavidin Alexa488. The coverslip DC's were BMDC's - BM cells were harveted from mice and grown with GMCSF for 5 days before transfering cells to PLL coated coverslips. Cells were allowed to attach for 2hrs and were then fixed with 4% PFA, 10min. Basically we want to look at DC's in the spleen in conjuction with a number of other cell markers. We are using CD11c and Dec205 antibodies but wanted an additional DC marker. Thanks for all your suggestions/comments Sonya From koellingr <@t> comcast.net Wed Feb 7 09:08:26 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Feb 7 09:08:34 2007 Subject: [Histonet] 33D1 DC marker- further info Message-ID: <020720071508.18892.45C9EB6A0002F0CD000049CC22070016419D09020704040A0105@comcast.net> Sorry, when talking about the cell-culture, of course I meant in-vitro and not in-vivo. Ray -------------- Original message -------------- From: koellingr@comcast.net > Sonya, > I guess my very greatest concern is from your first sentence. You did flow but > "didn't see anything". I guess I would never even attempt IHC if I didn't see > target verified by flow for an uncharacterized target. Mouse spleenocytes > stained with 33D1 do give a one log shift and there are enough of them you can > see them. This is not a shoulder off of the negative peak. I've seen numbers > of target sites per cell estimated at 10,000 molecules per cell so this should > be seen. Assuming you are not using a very strange strain of mouse since > haplotype restriction has not been fully assessed. As far as the BMDC's plated > to glass after GMCSF stimulation. Were these verified? In-vivo stimulations > sometimes do fail with resultant lack of expansion of the targeted population. > My cell culture experimental stimulations were never 100% successful. No ones > are due to multiple reasons. After 5 days in GMCSF, were those cells flowed? I > think originally you said the coverslip staining was > "weak" and that shouldn't be. Check them by flow first. Bad GMCSF, wrong > dilution calculation? Your stain setup looks good - it does and should work. > The markers CD11c and CD205 are fine and MIDC-8 is another. But I guess my > bottom line first and foremost is I wouldn't waste even another second doing IHC > until I can stain 33D1 in flow and on those coverslips just to be sure my > control standards are there and functioning. If you are flowing mouse > splenocytes, can pick up a dendritic cell population by CD11c or 205, and then > can't see any 33D1 at all - something is wrong long before IHC problems are > there. Bad mouse, bad 33D1, bad Alexa 488, bad something but it is a lot easier > to troubleshoot flow or coverslip staining of stimmed BMDC's and get that > working than to troubleshoot IHC staining up front. > Ray > > -------------- Original message -------------- > From: "Martin S." > > Hi All, > > Some further info; > > The sections were mouse spleen - we did some flow analysis with 33D1 of > splenocytes but didnt see anything - hard to know if this is because there are > just so few 33D1+ cells or if theres a problem with the antibody. There are a > few papers out there with quite a lot of staining with 33D1 in mouse spleen - > most recently in Science (Dudziak et al Vol 315 Jan 2007). For frozen sections I > have tried 33D1 + mouse anti-rat IgG2b:Biotin with Streptavidin Alexa488 and > also 33D1 + rb anti-rat:Biotin with tyramide amplification and Streptavidin > Alexa488. > The coverslip DC's were BMDC's - BM cells were harveted from mice and grown with > GMCSF for 5 days before transfering cells to PLL coated coverslips. Cells were > allowed to attach for 2hrs and were then fixed with 4% PFA, 10min. > Basically we want to look at DC's in the spleen in conjuction with a number of > other cell markers. We are using CD11c and Dec205 antibodies but wanted an > additional DC marker. > > Thanks for all your suggestions/comments > > Sonya > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Wed Feb 7 09:16:44 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed Feb 7 09:16:55 2007 Subject: [Histonet] Microwave Testing In-Reply-To: <57BE698966D5C54EAE8612E8941D76831998C1@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76831998C1@EXCHANGE3.huntingtonhospital.com> Message-ID: <45C9ED5C.8070005@ebsciences.com> Hello: I'm with a vendor, BUT - Although leakage measurement is always a good idea, on 12/12/2006, CAP's microwave leakage checklist item was deleted (without much fanfare, apparently). So the pressure's off for CAP-accredited labs. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway; please do the right thing and make it go away. Thank you. Laurie Colbert wrote: > I know this topic has been discussed before, but I can't find my info. What are others doing regarding the CAP questions regarding microwave leakage and temperature reproducibility? There is no one in my hospital that monitors microwaves - I will have to do it myself. Does anyone have info on ordering some kind of monitor? Do these questions apply only to those lab using microwaves for processing and/or staining? We will occasionally perform a special stain in the microwave (not regularly) - we normally just heat water, agar, etc. > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From Lchausse <@t> nmh.org Wed Feb 7 09:51:05 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Wed Feb 7 09:51:34 2007 Subject: [Histonet] Microwave Testing Message-ID: For those being inspected on AP CAP regs effective 12/06. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: Wednesday, February 07, 2007 9:17 AM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave Testing Hello: I'm with a vendor, BUT - Although leakage measurement is always a good idea, on 12/12/2006, CAP's microwave leakage checklist item was deleted (without much fanfare, apparently). So the pressure's off for CAP-accredited labs. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway; please do the right thing and make it go away. Thank you. Laurie Colbert wrote: > I know this topic has been discussed before, but I can't find my info. What are others doing regarding the CAP questions regarding microwave leakage and temperature reproducibility? There is no one in my hospital that monitors microwaves - I will have to do it myself. Does anyone have info on ordering some kind of monitor? Do these questions apply only to those lab using microwaves for processing and/or staining? We will occasionally perform a special stain in the microwave (not regularly) - we normally just heat water, agar, etc. > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From pex0220 <@t> yahoo.com.cn Wed Feb 7 09:55:08 2007 From: pex0220 <@t> yahoo.com.cn (docqian) Date: Wed Feb 7 09:55:22 2007 Subject: [Histonet] alkaline phosphatase antibody Message-ID: <122923.7945.qm@web15202.mail.cnb.yahoo.com> Dear all,=0A=0AI would like to use alkaline phosphatase antibody to label t= he early osteoblasts by immunofluorescence, however, I do not find a good a= ntibody which is suitable for my aim.=0AIf you have some ideas or you have = used it in your work, please give some suggestions.=0AThank you.=0A=0AGuofe= ng=0A=0A=0A=09=09=0A_______________________________________________________= ____ =0A=D1=C5=BB=A2=C3=E2=B7=D1=D3=CA=CF=E4-3.5G=C8=DD=C1=BF=A3=AC20M=B8= =BD=BC=FE =0Ahttp://cn.mail.yahoo.com/From denise.woodward <@t> uconn.edu Wed Feb 7 09:55:40 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Wed Feb 7 09:55:46 2007 Subject: [Histonet] ASCP Exam Long opinion References: Message-ID: Ditto for Community College of Rhode Island HT students! Denise Long Woodward -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, February 05, 2007 1:25 PM To: Joe Nocito Cc: Histonet@lists.utsouthwestern.edu; Edwards, R.E.; histonet-bounces@lists.utsouthwestern.edu; Rittman,Barry R; Jasper,Thomas G. Subject: Re: [Histonet] ASCP Exam Long opinion Joe, In our program students are required to do all staining by hand. This includes H&E's, specials, and IHC staining. They are also required to coverslip by hand. Jennifer "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/02/2007 05:02 PM To "Rittman, Barry R" , "Edwards, R.E." , "Jasper, Thomas G." cc Histonet@lists.utsouthwestern.edu Subject Re: [Histonet] ASCP Exam Long opinion I agree with you Barry. Techs need to know how the procedure is working to be able to troubleshoot when something happens. Is it the machine or the procedure? That's what I stressed when I was giving my intro to IHC lectures. Something intricate like immunos should be done manually until the tech is familiar with the procedure before they put slides on a machine. But, then again, I've been told that I'm a dinosaur. Oh well, it's Friday, I think I'll have a scotch. Enjoy Joe ----- Original Message ----- From: "Rittman, Barry R" To: "Edwards, R.E." ; "Jasper, Thomas G." Cc: Sent: Friday, February 02, 2007 8:59 AM Subject: RE: [Histonet] ASCP Exam Long opinion Nothing is black and white nowadays and I think that the current discussion on Histonet is important in putting forward all points of view.. I agree that automation is very important for producing consistent results and in many ways these have relieved us of tedious, repetitive tasks. I also respect the supervisors who are often stuck between a rock and a hard place. The problem I currently see is that in many cases these machines may be operated in a robotic fashion. If the individual operating the machine is knowledgeable about the process taking place be it histochemical, immunochemical or special stains and can take appropriate steps if problems arise then there should be no problem. Unfortunately in today's market, not just for histology, the emphasis appears to be on minimal qualifications to carry out a task. This may work perfectly well in those cases where instructions are concise and easily followed and solutions changed at specific intervals and times adhered to. However, I believe something is lost to the histotechs in such a situation. After all I operate my car with minimal knowledge of the mechanics and electronics. However were I to get paid to operate my car I would hope that I had time and make the effort to try understand the processes involved. I would consider this part of responsibilities and also a bonus in enhancing my work experience. My point is, are we going to "progress" into an era where we are just button pushers? This automation also releases individual so that many histotechs may carry out tasks that never used to be their responsibility. This appears to be a national trend. As an example, I am paid as a faculty member here but my time spent includes memos, filing etc. a task originally carried out by secretarial staff. It is not that I resent doing such tasks but after all I am paid more than secretaries and the "secretarial tasks" never appear anywhere in my yearly report of activities. Perhaps we will end up with histotechs plus a second group of button pushers? Perhaps secretaries will be reincarnated - probably not. I guess that I am from an era where work was varied and pleasurable rather than one in which the bottom line was the aim, and where employee and employees worked as a team. Hoe that this makes sense, this has been a one coffee morning so far. Barry -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Friday, February 02, 2007 8:09 AM To: Jasper, Thomas G.; Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Laboratory automation surely is as much due to obtaining reproducibility of results than saving on staff costs, as to date at least, all machines need human minders, who earn their corn when the machine malfunctions and for example they have to do a batch of H@Es by hand. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: 01 February 2007 18:17 To: Rittman, Barry R Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Exam Long opinion Hey Barry, I appreciate your long opinion, at times life requires long opinions. I agree with much of what you say and I will try to concisely explain how I see things. I totally agree that elimination of the practical was a bad idea. And I understand the arguments for getting rid of the practical. Let's start with automated staining. Unfortunately most labs in the clinical world of the US, Can., UK, Western Europe, S. Africa, Aus., NZ and Japan (sorry if I've omitted someone) have turned to this technology out of necessity. This is due to staff shortages and for cost containment. The consistency and reproducibility are greatly enhanced by reduction of variables (I know more science than art). But there you are, so a lab's automated stainer produces a stain, candidates submit. If this is the technology a candidate will likely use, that should be taken into consideration. I expect a candidate to cut their own sections. That would be subject to evaluation as it always was. And I expect someone to know what an H and E or any other stain is supposed to look like, automated or not. With so much emphasis on academics they should know what the stain looks like and why. Heck, with all this automation you could even expect a basic level of understanding about the mechanics involved and make that part of the written exam. Perhaps the elimination of the practical was not necessary, but a reassessment of the whole thing, or as you so cleverly stated "...what is needed for the entire system is a good enema!" I agree with the statements from others that they want to know that folks who are certified can cut sections. It is more balanced and despite all of our wonderful automation Histology is the one laboratory discipline that still requires a deft hand and an artistic eye. I'm not aware of any automated substitute for manual dexterity. I also agree with this whole ASCP/fox in the henhouse analogy of yours. I understand we've got good intentions here, but there is a mindset, amongst certain pathologists, about cheap labor. Despite pay increases in recent years (and I'm grateful) Histotechs overall, are the lowest paid laboratorians. Increased educational requirements (which I believe in) still have not eradicated this mindset. Please understand, I am not speaking about all pathologists, but there are enough to validate the analogy. Now Barry, I think your educational background is great and I suspect you're a better man because of it. It seems to me in this day and age that it would be near impossible to pull off. We've taken incredible leaps in technology just for Histology alone. I would be wary of an MLT or MT today that thought they could come into our Histology lab and perform at the level I expect (that whole dexterity thing again). And frankly, I think it would be extremely difficult to go into the General Lab, Blood Bank, Chemistry, Special Hematology, Microbiology or any other sub-discipline and perform at an acceptable level. Now do I think folks should have an understanding and appreciation for these other disciplines? Absolutely. And maybe that needs to be incorporated in Histology training at an academic level. But doing the work is another thing entirely. Anyway, I don't know if my opinion will count for much, but there you have it. It would be nice to see some changes and I think reinstating the practical is worth considering. I understand that logistical problems exist as well, along with some of the other subjective variables that Joe Nocito mentioned. Maybe judges could be sent regional sites or something. Anyway it's food for thought. Thanks for letting me ramble. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC - Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Feb 7 13:27:23 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 7 13:27:45 2007 Subject: [Histonet] alkaline phosphatase antibody In-Reply-To: <122923.7945.qm@web15202.mail.cnb.yahoo.com> Message-ID: <004101c74aed$feff7e90$6501a8c0@Patsy> I am not aware of an antibody for the alk phos enzyme histochemical detection we used to label osteoblasts, when I used this method it was a enzyme histochemical method not IHC. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of docqian Sent: Wednesday, February 07, 2007 8:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] alkaline phosphatase antibody Dear all, I would like to use alkaline phosphatase antibody to label the early osteoblasts by immunofluorescence, however, I do not find a good = antibody which is suitable for my aim. If you have some ideas or you have used it in your work, please give = some suggestions. Thank you. Guofeng =09 ___________________________________________________________=20 =D1=C5=BB=A2=C3=E2=B7=D1=D3=CA=CF=E4-3.5G=C8=DD=C1=BF=A3=AC20M=B8=BD=BC=FE= =20 http://cn.mail.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Feb 7 14:46:01 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Feb 7 14:46:15 2007 Subject: [Histonet] Histology in Texas, Tech Service and Training. Can you help? Message-ID: Hi Histonetters, I was hoping you could help me, I have a unique opportunity for someone interested in getting out of the lab. This is a Technical Service/Training Rep for a major histology services vendor. Do you know anyone who might be interested if so please pass my information along. If you might be interested please contact me at 866-607-3542 or relia1@earthlink.net Thanks for taking the time to read my e-mail. Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From PMonfils <@t> Lifespan.org Wed Feb 7 14:51:33 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Feb 7 14:51:46 2007 Subject: [Histonet] Vibratome sectioning of lung Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C23@LSRIEXCH1.lsmaster.lifespan.org> Has anyone sectioned lung on a vibratome or similar machine? I have used the vibratome for many years on various kinds of tissue, such as liver, kidney, brain, etc., both unembedded and embedded in agarose, agar, or gelatin. Generally speaking, few problems. But now someone has asked me to section some agarose-embedded mouse lungs and I'm not having any success at all. Regardless of what speed and oscillation settings I use and regardless of what type of knife I use, the tissue compresses on the knife edge instead of slicing, and eventually pulls out of the block. Any recommendations would be appreciated. From tncperfect2 <@t> comcast.net Wed Feb 7 19:39:32 2007 From: tncperfect2 <@t> comcast.net (tncperfect2@comcast.net) Date: Wed Feb 7 19:44:44 2007 Subject: [Histonet] Re: Histonet Digest, Vol 39, Issue 12 Message-ID: <020820070139.16617.45CA7F5400037685000040E92200735834CD9B0C0A009D0A9F0C029B@comcast.net> This message has been processed by Symantec's AntiVirus Technology. Unknown00000000.data was not scanned for viruses because too many nested levels of files were found. For more information on antivirus tips and technology, visit http://ses.symantec.com/ From wim.vandenbroeck <@t> UGent.be Thu Feb 8 02:01:07 2007 From: wim.vandenbroeck <@t> UGent.be (Prof. dr. Wim Van den Broeck) Date: Thu Feb 8 02:01:31 2007 Subject: [Histonet] tissue processor Message-ID: <001701c74b57$4a768df0$58b3c19d@PC20DI03> Dear colleagues, dear friends, We are lucky to have a budget to buy a new tissue processor. At the moment, we are interested in the following instruments (in alphabetical order): * Sakura Tissue-Tek VIP 5 Vacuum Infiltration processor * Shandon Excelsior * Leica ASP 300 advanced smart tissue processor * Microm STP 420 enclosed tissue processor I would like to hear your comments, experience with the different instruments, the pro's en con's, etc. Thanks in advance for your helpful reactions. Kind regards, Wim. --- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be From louise.renton <@t> gmail.com Thu Feb 8 05:16:11 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Feb 8 05:16:23 2007 Subject: [Histonet] recording images Message-ID: Hi all, I am interested to find out how other labs record images of gross specimens (usually complex ones)- primarily at grossing so that the blocks taken correlate to the actual specimen. When I was in a general lab the tech would have to sketch the specimen (shows how long ago that was - the Prof didn't even use gloves for put through) to show which margins etc were being sampled. thanks -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From abright <@t> brightinstruments.com Thu Feb 8 05:38:30 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Feb 8 05:37:02 2007 Subject: [Histonet] PMV450 Cryomicrotome Message-ID: I hope one of you can help me. I have had a very distressed UK technician contact me about a PMV450 Cryomicrotome (heavy duty, sledge motorised cabinet Cryomicrotome) that he is looking to get serviced and some user training too as the instrument is down at present. The well know company that should deal with this, will not support this request and are only interested in replacing it with a new ?150000 plus model. I have contacted other users in the same boat. Therefore I would like to hear from any of you that may know of a company somewhere in Europe that could fulfil this request to help these users out. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Feb 8 05:48:07 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Feb 8 05:48:20 2007 Subject: [Histonet] PMV450 Cryomicrotome Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124722E@wahtntex2.waht.swest.nhs.uk> There are loads of independent service companies that service these things, doesn't have to be the makers. I'm sure there was one in East Lancs that did it; if Dave at Queens Park still logs on then he may help, but if he doesn't give him a ring at the Pathology Department, Histology Department at Queens Park Hospital at Blackburn in East Lancs and I'm sure he will help. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com To be truly radical is to make hope possible, rather than despair convincing. --Raymond Williams This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From abright <@t> brightinstruments.com Thu Feb 8 06:08:15 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Feb 8 06:06:45 2007 Subject: [Histonet] PMV450 Cryomicrotome Message-ID: Dear Kemlo, Thank you for your prompt reply. I hope that Dave @ Queens Park still logs in to Histonet and contacts me, as the company in East Lancs (Themometric) is long gone and their service chap in Sussex is many years into his retirement. I know it does not have to be the makers that service this cryostat. As they are unwilling, my aim is to find one of these loads of independent service companies you mention, that can service the PMV450 Cryomicrotome. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 08 February 2007 11:48 To: Alan Bright; HistoNet Server Subject: RE: [Histonet] PMV450 Cryomicrotome There are loads of independent service companies that service these things, doesn't have to be the makers. I'm sure there was one in East Lancs that did it; if Dave at Queens Park still logs on then he may help, but if he doesn't give him a ring at the Pathology Department, Histology Department at Queens Park Hospital at Blackburn in East Lancs and I'm sure he will help. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com To be truly radical is to make hope possible, rather than despair convincing. --Raymond Williams This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From lblazek <@t> digestivespecialists.com Thu Feb 8 06:21:41 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Feb 8 06:22:08 2007 Subject: [Histonet] tissue processor References: <001701c74b57$4a768df0$58b3c19d@PC20DI03> Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684D17@bruexchange.digestivespecialists.com> I have had the Microm STP 420 for 6 weeks now and we love it. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim Van den Broeck Sent: Thursday, February 08, 2007 3:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue processor Dear colleagues, dear friends, We are lucky to have a budget to buy a new tissue processor. At the moment, we are interested in the following instruments (in alphabetical order): * Sakura Tissue-Tek VIP 5 Vacuum Infiltration processor * Shandon Excelsior * Leica ASP 300 advanced smart tissue processor * Microm STP 420 enclosed tissue processor I would like to hear your comments, experience with the different instruments, the pro's en con's, etc. Thanks in advance for your helpful reactions. Kind regards, Wim. --- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beldorth.msu+hist <@t> gmail.com Thu Feb 8 09:01:18 2007 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Thu Feb 8 09:01:29 2007 Subject: [Histonet] Is Nicole Schmitz still around? Message-ID: Hi All, I am trying to locate Nicole Schmitz in regards to an in Situ problem she encountered several years ago using NBT/BCIP for Alkaline Phosphatase detection. Can anyone help me locate her? Thanks, Ion From gcallis <@t> montana.edu Thu Feb 8 09:07:09 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Feb 8 09:07:24 2007 Subject: [Histonet] Replies to Histonet Digest make Symantec's antivirus technology very unhappy! In-Reply-To: <020820070139.16617.45CA7F5400037685000040E92200735834CD9B0 C0A009D0A9F0C029B@comcast.net> References: <020820070139.16617.45CA7F5400037685000040E92200735834CD9B0C0A009D0A9F0C029B@comcast.net> Message-ID: <6.0.0.22.1.20070208080347.01b2d0f0@gemini.msu.montana.edu> When Histonetters reply to the digest, the backlog of messages for that day are included in their message. Symantec antivirus did NOT like this, consequently, if you needed an answer or are replying to a specific subject line, I suggest you do NOT reply to the digest, but send a message directly to Histonet. At 06:39 PM 2/7/2007, you wrote: >This message has been processed by Symantec's AntiVirus Technology. > >Unknown00000000.data was not scanned for viruses because too many nested >levels of files were found. > > >For more information on antivirus tips and technology, visit >http://ses.symantec.com/_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From timothy.macatee <@t> med.nyu.edu Thu Feb 8 09:24:34 2007 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Thu Feb 8 09:26:11 2007 Subject: [Histonet] Processing.. In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E4031@exchsrv01.barrynet.barry.edu> Message-ID: Hi Everyone, I'm working with a new faculty member here who is doing fluorescent immunohistochemistry with tiramide amplification. We've been troubleshooting the histology that we've been doing for him, so he can get the same results he got at his old institution. We are following the same processing schedule for his spleen, and lymph nodes that his old place did. Recently, however, we found that the old place was doing manual processing with 30 minute steps for dehydration and clearing. Barring any inherent problems that always seem to happen when you move labs, or what I like to call SMURFS (Small Molecular Un-reactive Factors). Does the same schedule in the processor compare in any way to a manual processing? Should I shorten things up? Also, I've been changing solutions in the processor after 250 to 300 cassettes are run. Would changing solutions more often improve the chances of getting better immuno results? Thanks. Tim -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From lubit1sr <@t> cmich.edu Thu Feb 8 09:34:13 2007 From: lubit1sr <@t> cmich.edu (Sarah R Lubitz) Date: Thu Feb 8 09:34:26 2007 Subject: [Histonet] CMU Microscopy Grad Looking for Job Opportunities in Florida Message-ID: <20070208103413.AEL59635@student-e.cmich.edu> I will be graduating from Central Michigan University in May '07 with a B.S. in biology with an emphasis in microscopy. After graduation I will be moving to Orlando, FL. I would like to know of any job opportunities that would be available in this area. Please contact me with any suggestions or helpful hints. Thanks Sarah Lubitz Central Michigan University Mt. Pleasant, MI Lubit1sr@cmich.edu From lesley.bechtold <@t> jax.org Thu Feb 8 10:30:11 2007 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Thu Feb 8 10:32:12 2007 Subject: [Histonet] portable darkrooms Message-ID: <20070208113011463.00000006020@spikey> If anyone has any information on small, stand-alone darkrooms, I would appreciate hearing from you. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From ree3 <@t> leicester.ac.uk Thu Feb 8 10:40:01 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Feb 8 10:40:16 2007 Subject: [Histonet] portable darkrooms In-Reply-To: <20070208113011463.00000006020@spikey> References: <20070208113011463.00000006020@spikey> Message-ID: A tent, covered with lightproof curtain linings?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: 08 February 2007 16:30 To: Histology Network Subject: [Histonet] portable darkrooms If anyone has any information on small, stand-alone darkrooms, I would appreciate hearing from you. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 8 10:54:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 8 10:54:22 2007 Subject: [Histonet] tissue processor In-Reply-To: <001701c74b57$4a768df0$58b3c19d@PC20DI03> Message-ID: <521365.56061.qm@web61224.mail.yahoo.com> Dear Prof. van den Broek: During my career I have used many tissue processors and the one I would recommed you is the Sakura VIP (any model/capacity). It has been for me the most reliable and because of high quality processing. It is what we call here "a work horse". Ren? J. "Prof. dr. Wim Van den Broeck" wrote: Dear colleagues, dear friends, We are lucky to have a budget to buy a new tissue processor. At the moment, we are interested in the following instruments (in alphabetical order): * Sakura Tissue-Tek VIP 5 Vacuum Infiltration processor * Shandon Excelsior * Leica ASP 300 advanced smart tissue processor * Microm STP 420 enclosed tissue processor I would like to hear your comments, experience with the different instruments, the pro's en con's, etc. Thanks in advance for your helpful reactions. Kind regards, Wim. --- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From mgolbaba <@t> uoguelph.ca Thu Feb 8 11:08:15 2007 From: mgolbaba <@t> uoguelph.ca (mgolbaba@uoguelph.ca) Date: Thu Feb 8 11:08:34 2007 Subject: [Histonet] Who knows? Message-ID: <20070208120815.k6wtq519q8ssk8c4@webmail.uoguelph.ca> Hi Everyone, I want to remove LR white resin from my sample (wheat straw) for staining. Please let me know how I can do it? What is the best method? Thanks a lot. Best Regards, Mahsa From alaskagirl1950 <@t> yahoo.com Thu Feb 8 12:15:44 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Feb 8 12:15:55 2007 Subject: [Histonet] tissue processor In-Reply-To: <521365.56061.qm@web61224.mail.yahoo.com> Message-ID: <249138.65084.qm@web52501.mail.yahoo.com> I totally agree, the VIP is a wonderful long lived machine, out lives replacement parts. Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. http://tools.search.yahoo.com/toolbar/features/mail/ From sbreeden <@t> nmda.nmsu.edu Thu Feb 8 12:53:27 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Feb 8 12:53:36 2007 Subject: [Histonet] Joe Nocito, please Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB476@nmdamailsvr.nmda.ad.nmsu.edu> Joe, would you be kind enough to email me so I can contact you directly; my subject is the NM Society for Histology membership. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From atebo <@t> aahs.org Thu Feb 8 13:18:44 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Thu Feb 8 13:19:03 2007 Subject: [Histonet] tissue processor In-Reply-To: <521365.56061.qm@web61224.mail.yahoo.com> References: <001701c74b57$4a768df0$58b3c19d@PC20DI03> <521365.56061.qm@web61224.mail.yahoo.com> Message-ID: <00790F10D0600A41AEE8899901E533A61044B48A@aamcexch.aamc.org> I agree! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 08, 2007 11:54 AM To: wim.vandenbroeck@UGent.be; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue processor Dear Prof. van den Broek: During my career I have used many tissue processors and the one I would recommed you is the Sakura VIP (any model/capacity). It has been for me the most reliable and because of high quality processing. It is what we call here "a work horse". Ren? J. "Prof. dr. Wim Van den Broeck" wrote: Dear colleagues, dear friends, We are lucky to have a budget to buy a new tissue processor. At the moment, we are interested in the following instruments (in alphabetical order): * Sakura Tissue-Tek VIP 5 Vacuum Infiltration processor * Shandon Excelsior * Leica ASP 300 advanced smart tissue processor * Microm STP 420 enclosed tissue processor I would like to hear your comments, experience with the different instruments, the pro's en con's, etc. Thanks in advance for your helpful reactions. Kind regards, Wim. --- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From Jason.Wiese <@t> va.gov Thu Feb 8 13:31:25 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Thu Feb 8 13:31:52 2007 Subject: [Histonet] tissue processor In-Reply-To: <001701c74b57$4a768df0$58b3c19d@PC20DI03> References: <001701c74b57$4a768df0$58b3c19d@PC20DI03> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E127CE@VHAV20MSGA3.v20.med.va.gov> Hello Sir: I would suggest a demo of at least two before you decide... I purchased an Excelsior from Shandon about 8 months ago, and I am very happy with it. I understand your position as I work for a VA, and though the government loves to spend money... it is hard to get them to spend it on equipment to better care for our veterans rather then on bombers and the payloads they drop. Anyway, I would suggest doing a demo of a couple different processors. I have worked with the Sakura VIP processors for years. I do like them as well, but they waste a lot of reagent. The Excelsior works on specific gravity and auto rotates. This means you get maximum usage out of you reagents. It has cut my usage of processing reagents in half. I wouldn't trade it for two VIP's. My 2 cents... Jason Jason E. Wiese, BS, HT(ASCP) VAROS Histology/Cytology 913 NW Garden Valley Blvd. Roseburg, OR 97470 (541)440-1000 ext. 44751 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim Van den Broeck Sent: Thursday, February 08, 2007 12:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue processor Dear colleagues, dear friends, We are lucky to have a budget to buy a new tissue processor. At the moment, we are interested in the following instruments (in alphabetical order): * Sakura Tissue-Tek VIP 5 Vacuum Infiltration processor * Shandon Excelsior * Leica ASP 300 advanced smart tissue processor * Microm STP 420 enclosed tissue processor I would like to hear your comments, experience with the different instruments, the pro's en con's, etc. Thanks in advance for your helpful reactions. Kind regards, Wim. --- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakerj <@t> umich.edu Thu Feb 8 14:04:31 2007 From: bakerj <@t> umich.edu (John Baker) Date: Thu Feb 8 14:09:06 2007 Subject: [Histonet] Fwd: Osteoblast Staining Message-ID: <0fe97b843954cd7339d9807657148088@umich.edu> One of our graduate students has a question that I hoped someone in histonetland might be able to answer from their experience. Thank you in advance, John > > Subject: Osteoblast Staining > > I have been trying to do Alk.Phos. staining of paraffin embedded, EDTA > decalcified rat tibiae, using the Sigma kit #86 without success. I was > wondering if anyone has had any success with this? Also, I was > wondering if anyone had tried to do osteoblast staining with X-Gal, or > any other stain? Thank you for your assistance! Erik Waldorff > > > John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From AGrobe2555 <@t> aol.com Thu Feb 8 14:30:30 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Feb 8 14:30:49 2007 Subject: [Histonet] Re:Osteoblast staining Message-ID: John, I don't have a good answer, but instead a question. Does Alkaline phosphatase survive de-cal and paraffin processing? It seems that all/most enzymatic activity would be killed by this processing..... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From bwhitaker <@t> brownpathology.com Thu Feb 8 14:36:07 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Feb 8 14:31:16 2007 Subject: [Histonet] tissue processor In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E127CE@VHAV20MSGA3.v20.med.va.gov> Message-ID: <000601c74bc0$c2ca2460$3601a8c0@brownpathology.net> I agree!! I love my 2 Excelsiors!! I've had them for about 3 years. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Thursday, February 08, 2007 1:31 PM To: wim.vandenbroeck@UGent.be; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissue processor Hello Sir: I would suggest a demo of at least two before you decide... I purchased an Excelsior from Shandon about 8 months ago, and I am very happy with it. I understand your position as I work for a VA, and though the government loves to spend money... it is hard to get them to spend it on equipment to better care for our veterans rather then on bombers and the payloads they drop. Anyway, I would suggest doing a demo of a couple different processors. I have worked with the Sakura VIP processors for years. I do like them as well, but they waste a lot of reagent. The Excelsior works on specific gravity and auto rotates. This means you get maximum usage out of you reagents. It has cut my usage of processing reagents in half. I wouldn't trade it for two VIP's. My 2 cents... Jason Jason E. Wiese, BS, HT(ASCP) VAROS Histology/Cytology 913 NW Garden Valley Blvd. Roseburg, OR 97470 (541)440-1000 ext. 44751 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim Van den Broeck Sent: Thursday, February 08, 2007 12:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue processor Dear colleagues, dear friends, We are lucky to have a budget to buy a new tissue processor. At the moment, we are interested in the following instruments (in alphabetical order): * Sakura Tissue-Tek VIP 5 Vacuum Infiltration processor * Shandon Excelsior * Leica ASP 300 advanced smart tissue processor * Microm STP 420 enclosed tissue processor I would like to hear your comments, experience with the different instruments, the pro's en con's, etc. Thanks in advance for your helpful reactions. Kind regards, Wim. --- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Thu Feb 8 14:39:17 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Feb 8 14:39:35 2007 Subject: [Histonet] iNOS control tissue Message-ID: I will be staining sheep tissues for iNOS and would like to include a positive sheep control tissue to verify antibody specificity. Would spleen be adequate, or is there a better tissue? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From dfinkelstein <@t> mhri.edu.au Thu Feb 8 15:59:00 2007 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Thu Feb 8 15:59:37 2007 Subject: [Histonet] Vibratome sectioning of lung Message-ID: <000801c74bcc$56d5eb20$16a503cb@davidfink> Hi Paul Have you tried mounting your block stuck in front of to a piece of polystyrene foam? The foam won't damage the blade and limits the movement of the tissue block. Regards David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA dfinkelstein@mhri.edu.au From Barry.R.Rittman <@t> uth.tmc.edu Thu Feb 8 16:07:21 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Feb 8 16:07:30 2007 Subject: [Histonet] Re:Osteoblast staining Message-ID: I believe that with most types of fixation and processing that alkaline phosphatase is destroyed. However, if memory serves me correctly, a method was used in which tissue was fixed in acetone and straight into wax. Short time in wax. Believe that it was stated that 70% of the alkaline phosphatase was preserved. I did carry out this method on kidney and it was successful. However, tissue is difficult to cut when fixed and processed in this manner. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Thursday, February 08, 2007 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Osteoblast staining John, I don't have a good answer, but instead a question. Does Alkaline phosphatase survive de-cal and paraffin processing? It seems that all/most enzymatic activity would be killed by this processing..... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrsseagle <@t> yahoo.com Thu Feb 8 16:42:10 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Thu Feb 8 16:42:21 2007 Subject: [Histonet] ASCP EXAM Message-ID: <97400.41652.qm@web51810.mail.yahoo.com> I was referring to the online study questions that you can purchase from the ascp website. The freida carson practice question booklet is wonderful. Michelle Seagle HT (ASCP) Rutherford Hospital Rutherfordton NC Michelle, I am not sure about what you mean that the ASCP practical questions are waste of time, I am pretty sure that Freida Carson wrote those and they are very similar to the questions in her book. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE SEAGLE Sent: Monday, January 29, 2007 7:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP EXAM I just took HT exam this past december and I dont remember having any IHC related questions on the exam. You just need to know the freida carson book backwards and forwards because they pick the starngest things out of that book that you would not know unless you have just practically memorized the book!! Don't wast your time or money on the ASCP practical exam questions, what a waste. As far as the practical part I am not sure but I believe It has been Removed from the requirements since everthing is so automated now. Good Luck on your Exam Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From mbeaucha <@t> benaroyaresearch.org Thu Feb 8 16:57:02 2007 From: mbeaucha <@t> benaroyaresearch.org (Mary Beauchamp) Date: Thu Feb 8 16:52:16 2007 Subject: [Histonet] formalin fixed mouse ears-help Message-ID: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> Hi there, I'm having problems with 10% neutral buffered formalin fixed mouse ears embedded in paraffin. They are splitting from the cartilage as soon, and I mean, as soon as they hit the water bath. I'm open to any suggestions, no matter how silly or simple. This is a chronic problem and these mouse ears will continue to be submitted to me for a long time. Please help! -Mary B _____ I am using the free version of SPAMfighter for private users. It has removed 78 spam emails to date. Paying users do not have this message in their emails. Try SPAMfighter for free now! From pruegg <@t> ihctech.net Thu Feb 8 17:01:22 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Feb 8 17:01:43 2007 Subject: [Histonet] iNOS control tissue In-Reply-To: Message-ID: <200702082301.l18N1JqR025043@pro12.abac.com> I do iNOS, I will check to see what is recommended for a + control when I get back to work tomorrow. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Thursday, February 08, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] iNOS control tissue I will be staining sheep tissues for iNOS and would like to include a positive sheep control tissue to verify antibody specificity. Would spleen be adequate, or is there a better tissue? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Feb 8 17:04:32 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Feb 8 17:04:52 2007 Subject: [Histonet] Fwd: Osteoblast Staining In-Reply-To: <0fe97b843954cd7339d9807657148088@umich.edu> Message-ID: <200702082304.l18N4T4d026701@pro12.abac.com> We had to process bone with cold methyl alcohol fixation into glycol methacrylate (GMA) without decal to get the enzyme alk phos method to work. It is also very sensitive to heat so you really have to avoid heat during polymerization and other times. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Baker Sent: Thursday, February 08, 2007 1:05 PM To: Histonet@pathology.swmed.edu Cc: ewaldorf@engin.umich.edu Subject: [Histonet] Fwd: Osteoblast Staining One of our graduate students has a question that I hoped someone in histonetland might be able to answer from their experience. Thank you in advance, John > > Subject: Osteoblast Staining > > I have been trying to do Alk.Phos. staining of paraffin embedded, EDTA > decalcified rat tibiae, using the Sigma kit #86 without success. I was > wondering if anyone has had any success with this? Also, I was > wondering if anyone had tried to do osteoblast staining with X-Gal, or > any other stain? Thank you for your assistance! Erik Waldorff > > > John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Feb 8 17:10:03 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Feb 8 17:10:11 2007 Subject: [Histonet] formalin fixed mouse ears-help In-Reply-To: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> Message-ID: <200702082310.l18NA03E029815@pro12.abac.com> Cartilage does separate from the rest of the tissue very easily, you may need to process in GMA, even in GMA it can be a problem. I had to make elmers glue coated slides (dip in a 5% solution in dih20, then airdry slides overnight before using them to pick up sentions) dry the sections flat at 37dc overnight. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Beauchamp Sent: Thursday, February 08, 2007 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin fixed mouse ears-help Hi there, I'm having problems with 10% neutral buffered formalin fixed mouse ears embedded in paraffin. They are splitting from the cartilage as soon, and I mean, as soon as they hit the water bath. I'm open to any suggestions, no matter how silly or simple. This is a chronic problem and these mouse ears will continue to be submitted to me for a long time. Please help! -Mary B _____ I am using the free version of SPAMfighter for private users. It has removed 78 spam emails to date. Paying users do not have this message in their emails. Try SPAMfighter for free now! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Feb 8 17:12:22 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Feb 8 17:12:34 2007 Subject: [Histonet] Fwd: Osteoblast Staining In-Reply-To: <200702082304.l18N4T4d026701@pro12.abac.com> Message-ID: <200702082312.l18NCJsu030925@pro12.abac.com> Another way to preserve alk phos enzyme in osteoblasts is to do frozen sections on unfixed bone with the tape transfer system of course. This method yields so much alk phos it is over whelming. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy ruegg Sent: Thursday, February 08, 2007 4:05 PM To: 'John Baker'; Histonet@pathology.swmed.edu Cc: ewaldorf@engin.umich.edu Subject: RE: [Histonet] Fwd: Osteoblast Staining We had to process bone with cold methyl alcohol fixation into glycol methacrylate (GMA) without decal to get the enzyme alk phos method to work. It is also very sensitive to heat so you really have to avoid heat during polymerization and other times. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Baker Sent: Thursday, February 08, 2007 1:05 PM To: Histonet@pathology.swmed.edu Cc: ewaldorf@engin.umich.edu Subject: [Histonet] Fwd: Osteoblast Staining One of our graduate students has a question that I hoped someone in histonetland might be able to answer from their experience. Thank you in advance, John > > Subject: Osteoblast Staining > > I have been trying to do Alk.Phos. staining of paraffin embedded, EDTA > decalcified rat tibiae, using the Sigma kit #86 without success. I was > wondering if anyone has had any success with this? Also, I was > wondering if anyone had tried to do osteoblast staining with X-Gal, or > any other stain? Thank you for your assistance! Erik Waldorff > > > John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbeaucha <@t> benaroyaresearch.org Thu Feb 8 17:51:59 2007 From: mbeaucha <@t> benaroyaresearch.org (Mary Beauchamp) Date: Thu Feb 8 17:47:13 2007 Subject: [Histonet] mouse ears again Message-ID: <0b62fbde392bdd47b3f25a063a0f0e39@localhost.localdomain> Thanks for the replies so far. I generally have my water bath temp at 38-40 C. Although with the splitting problem, I've reduced the temp dramatically. It's been suggested that I use a detergent, Tween for example, diluted in room temp water, and float the section on a slide and gently heat the slide on a slide warmer. I have also played with a few different fixatives, and carefully controlled the fixation time before processing. I use a Leica ASP 300 processor and change the solutions on a regular basis. Keep the suggestions coming, please. -Mary _____ I am using the free version of SPAMfighter for private users. It has removed 78 spam emails to date. Paying users do not have this message in their emails. Try SPAMfighter for free now! From marjoh3 <@t> telus.net Thu Feb 8 18:02:42 2007 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Thu Feb 8 18:03:37 2007 Subject: [Histonet] Thermoplastic Cementing of Otoliths Message-ID: <000a01c74bdd$a02eb660$6401a8c0@VALUED20606295> Hi Histonetters, A University student that is currently working in our Fish & Wildlife Lab is doing some work with embedding Fat Head Minnow otoliths on a slide. They are trying to get a layer on top of the original embedded layer to bond together. The otoliths first have to be "sanded" in the original layer before they try to add the top layer. Any info from someone using this technique would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada From kathyg <@t> eoni.com Thu Feb 8 18:13:01 2007 From: kathyg <@t> eoni.com (Kathy Gorham) Date: Thu Feb 8 18:13:13 2007 Subject: [Histonet] Grossing Stations References: FB239D467A25DE40A08FADAA3192FD020174950C@oaintsedb1.ssfhs.org Message-ID: <003d01c74bdf$130148f0$693ffea9@kathy83b707eca> We have a 600 Mopec grossing station also but we are having some problem with the ventilation. Is your self contained or is it vented into your hospital vent system? Our's is self contained. Is anyone having the same problem or have any suggestions? Thanks Kathy Gorham Supervisor Histology Grande Ronde Hospital LaGrande Or ----- Original Message ----- From: "Temple Nancy" To: Sent: Friday, January 26, 2007 6:01 AM Subject: RE: [Histonet] Grossing Stations When we remodeled our lab, we put in Mopec grossing stations. The company is great to work with. The stations can be built to suit your needs. We have one, I believe it is the 600, that is height adjustable. The other unit is stationary. Ventilation works well on both. Would highly recommend Mopec. Nancy Temple Supervisor Histology/Cytology St. Francis Hospital Indianapolis, In -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Thursday, January 25, 2007 6:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Stations All, We are considering purchasing two large grossing stations with some extra options. Can any of you recommend the grossing stations that you would prefer. We would like the grossing station to last for a reasonable amount of time, be sturdy in construction, offer options that can be added later but also be user friendly with enough shelf space and work area. Most importantly the ventilation must be of high quality. We have several companies that we are looking at but would like to get an idea of what most folks are using and if you are happy with the units or not. If you could also tell us what features you liked. Thank you in advance, Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Thu Feb 8 21:54:46 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Thu Feb 8 21:54:53 2007 Subject: [Histonet] Grossing Stations Message-ID: <445944.81820.qm@web58909.mail.re1.yahoo.com> Kathy, Are you smelling formalin in the lab? If so, check the condition of the filter, or check when the last time the filter was changed. The filters in the Mopec self-contained ventilation systems contain potassium iodide to trap formalin vapors. Maybe the filter is exhausted. Just a thought... Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Kathy Gorham To: Temple Nancy ; histonet@lists.utsouthwestern.edu Sent: Thursday, February 8, 2007 5:13:01 PM Subject: Re: [Histonet] Grossing Stations We have a 600 Mopec grossing station also but we are having some problem with the ventilation. Is your self contained or is it vented into your hospital vent system? Our's is self contained. Is anyone having the same problem or have any suggestions? Thanks Kathy Gorham Supervisor Histology Grande Ronde Hospital LaGrande Or ----- Original Message ----- From: "Temple Nancy" To: Sent: Friday, January 26, 2007 6:01 AM Subject: RE: [Histonet] Grossing Stations When we remodeled our lab, we put in Mopec grossing stations. The company is great to work with. The stations can be built to suit your needs. We have one, I believe it is the 600, that is height adjustable. The other unit is stationary. Ventilation works well on both. Would highly recommend Mopec. Nancy Temple Supervisor Histology/Cytology St. Francis Hospital Indianapolis, In -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Thursday, January 25, 2007 6:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Stations All, We are considering purchasing two large grossing stations with some extra options. Can any of you recommend the grossing stations that you would prefer. We would like the grossing station to last for a reasonable amount of time, be sturdy in construction, offer options that can be added later but also be user friendly with enough shelf space and work area. Most importantly the ventilation must be of high quality. We have several companies that we are looking at but would like to get an idea of what most folks are using and if you are happy with the units or not. If you could also tell us what features you liked. Thank you in advance, Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/newmail_tools.html From ryaskovich <@t> dir.nidcr.nih.gov Fri Feb 9 08:32:52 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Feb 9 08:33:05 2007 Subject: [Histonet] formalin fixed mouse ears-help In-Reply-To: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> References: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> Message-ID: Mary, If you can give them a decal soak. I use Immunocal. I keep playing with the water bath temperature and then take each section one at a time. Mine are not splitting at all but they are fixed in 4% Para. (which I don't think matters for the cutting) Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neurobiology and Pain Therapeutics Branch -----Original Message----- From: Mary Beauchamp [mailto:mbeaucha@benaroyaresearch.org] Sent: Thursday, February 08, 2007 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin fixed mouse ears-help Hi there, I'm having problems with 10% neutral buffered formalin fixed mouse ears embedded in paraffin. They are splitting from the cartilage as soon, and I mean, as soon as they hit the water bath. I'm open to any suggestions, no matter how silly or simple. This is a chronic problem and these mouse ears will continue to be submitted to me for a long time. Please help! -Mary B _____ I am using the free version of SPAMfighter for private users. It has removed 78 spam emails to date. Paying users do not have this message in their emails. Try SPAMfighter for free now! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atebo <@t> aahs.org Fri Feb 9 08:36:28 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Fri Feb 9 08:38:49 2007 Subject: [Histonet] FW: Leica Coverslipper Message-ID: <00790F10D0600A41AEE8899901E533A61044B497@aamcexch.aamc.org> > ______________________________________________ > From: Tebo, Andrea > Sent: Thursday, February 08, 2007 2:26 PM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: Leica Coverslipper > > > We are looking at replacing our Sakura tape coverslipper and we have > the Leica Autostainer XL for our H&E staining. Does anyone have the > coverslipper that attaches to the Autostainer XL? How does it work > and do you like it? > > Thanks for your time and input, > > Andrea Tebo > Supervisor Pathology > Anne Arundel Medical Center > 443-481-4240 > ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From jqb7 <@t> cdc.gov Fri Feb 9 08:51:27 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Feb 9 08:57:26 2007 Subject: [Histonet] FW: Leica Coverslipper References: <00790F10D0600A41AEE8899901E533A61044B497@aamcexch.aamc.org> Message-ID: We have it and it works beautifully! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tebo, Andrea Sent: Friday, February 09, 2007 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Leica Coverslipper > ______________________________________________ > From: Tebo, Andrea > Sent: Thursday, February 08, 2007 2:26 PM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: Leica Coverslipper > > > We are looking at replacing our Sakura tape coverslipper and we have > the Leica Autostainer XL for our H&E staining. Does anyone have the > coverslipper that attaches to the Autostainer XL? How does it work > and do you like it? > > Thanks for your time and input, > > Andrea Tebo > Supervisor Pathology > Anne Arundel Medical Center > 443-481-4240 > ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Feb 9 09:03:04 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Feb 9 09:09:57 2007 Subject: [Histonet] FW: Leica Coverslipper References: <00790F10D0600A41AEE8899901E533A61044B497@aamcexch.aamc.org> Message-ID: Sorry, I didn't answer your whole question. You must purchase the coverslipper and a separate transfer station. You then program your H&E stainer to end at the coverslip station. Once it completes it's time in the last xylene the rack is carried to the transfer station where it is loaded onto the coverslipper. It coverslips the slides and there they are waiting for you in the output rack. No contact with the xylene from beginning to end. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, February 09, 2007 9:51 AM To: Tebo, Andrea; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Leica Coverslipper We have it and it works beautifully! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tebo, Andrea Sent: Friday, February 09, 2007 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Leica Coverslipper > ______________________________________________ > From: Tebo, Andrea > Sent: Thursday, February 08, 2007 2:26 PM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: Leica Coverslipper > > > We are looking at replacing our Sakura tape coverslipper and we have > the Leica Autostainer XL for our H&E staining. Does anyone have the > coverslipper that attaches to the Autostainer XL? How does it work > and do you like it? > > Thanks for your time and input, > > Andrea Tebo > Supervisor Pathology > Anne Arundel Medical Center > 443-481-4240 > ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> slhduluth.com Fri Feb 9 09:12:34 2007 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Fri Feb 9 09:12:50 2007 Subject: [Histonet] FW: Leica Coverslipper In-Reply-To: <00790F10D0600A41AEE8899901E533A61044B497@aamcexch.aamc.org> Message-ID: We just got the coverslipper - it replaces the older model CV5000 that served in the department for 9 years. We will be installing the multi-stainer and transfer station soon. The coverslipper works very well. Our pathologists and storage conditions dictate that we use glass coverslips. I would recommend the Leica. Sheila Tapper HT(ASCP) Histology Technical Specialist St. Luke's Hospital Duluth, MN 55805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tebo, Andrea Sent: Friday, February 09, 2007 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Leica Coverslipper > ______________________________________________ > From: Tebo, Andrea > Sent: Thursday, February 08, 2007 2:26 PM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: Leica Coverslipper > > > We are looking at replacing our Sakura tape coverslipper and we have > the Leica Autostainer XL for our H&E staining. Does anyone have the > coverslipper that attaches to the Autostainer XL? How does it work > and do you like it? > > Thanks for your time and input, > > Andrea Tebo > Supervisor Pathology > Anne Arundel Medical Center > 443-481-4240 > ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Feb 9 09:20:13 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Feb 9 09:20:27 2007 Subject: [Histonet] formalin fixed mouse ears-help In-Reply-To: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> References: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> Message-ID: <6.0.0.22.1.20070209080907.01b28190@gemini.msu.montana.edu> Mary, Since you did not say what your processing schedule is for these ears, I suspect infiltration in paraffin is not very good so extend the time there and use a vacuum. This still denser tissue even though it appears very thin, and those layers can be a problem. Try at least three changes under vacuum. Hopefully you have vacuum and pressure on your processor, and don't add heat to the processing solvents, these are skinny dry tissues to begin with. 4. Lower the temperature of the water bath and don't over soak a trimmed block on ice water. Nothing is silly or simple if you are having problems. Good luck >I'm having problems with 10% neutral buffered formalin fixed mouse ears >embedded in paraffin. They are splitting from the cartilage as soon, and >I mean, as soon as they hit the water bath. > >I'm open to any suggestions, no matter how silly or simple. This is a >chronic problem and these mouse ears will continue to be submitted to me >for a long time. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From galinadeyneko <@t> yahoo.com Fri Feb 9 09:51:32 2007 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Fri Feb 9 09:51:44 2007 Subject: [Histonet] smooth muscle cell actin for rabbit Message-ID: <842576.99403.qm@web33114.mail.mud.yahoo.com> Dear Colleagues, I am looking for primary antibody against smooth muscle cell actin in FFPE rabbit aorta with athero lesion. I have used the mouse monoclonal primary AB from Biocare #CM001, clone 1A4, reacts with human ,rat, rabbit etc. with following protocol: No pre-treatment. Peroxidase blocking Protein blocking Incubation with AB 2 hours at RT Detection with MACH 2 polymer -HRP (Biocare) Chromogen: DAB. AB gives non-specific staining in macrophages, and foam cells, and SMC are stained light brown. With mouse aorta and sinus with MOM kit this AB works very well. The same results with monkey's coronary arteries. Any information and protocols for both species are highly appreciated. Sincerely. Galina Deyneko CardioVasccular Department Novartis, Cambridge, MA 617-871-7613. --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From corycody <@t> msn.com Fri Feb 9 10:26:49 2007 From: corycody <@t> msn.com (Bly Haverland) Date: Fri Feb 9 10:27:04 2007 Subject: [Histonet] 2007 Region III meeting in NC Message-ID: The North Carolina Society of Histopathology Technologists is pleased to announce it will be hosting the 2007 Region III meeting this year taking place March 22-24, 2007 in Research Triangle Park, North Carolina at the Raleigh-Durham Airport Hilton. The Society invites anyone interested in histology to attend. Membership is not required for participation. The officers and volunteers of NCSHT have put together an exciting program this year, and they look forward to as many participants as possible. A complete listing of the Region III program along with registration forms is posted on the NSH website at www.nsh.org Thank you. Bly Haverland HT, HTL Triangle Histology Services, Inc. corycody@msn.com From cnunes <@t> ipimar.pt Fri Feb 9 10:27:09 2007 From: cnunes <@t> ipimar.pt (Cristina Nunes) Date: Fri Feb 9 10:27:46 2007 Subject: [Histonet] AFA fixative Message-ID: <200702091631.l19GVtPK002913@neptuno.ipimar.pt> Dear histoneters, For several years, in the Institute where I am working, the main fixative used for the preservation of gonads of fish was AFA, with apparently good results. Then, at a given moment (before I arrived to the Institute), it was decided to change from AFA to a formalin solution because of the higher toxicity of the former. The histological results were slightly less good but for practical reasons (most fish tissue fixations occur at sea, on board the research vessels), it was considered to be the best solution. However, nobody was able to explain me why AFA is much more toxic than a formalin solution (I am sorry for my ignorance in terms of chemistry). Could anyone clarify me on that point? Many thanks in advance and a very nice week-end. Regards, Cristina Nunes. ><<<> ......................................................................><<<> Cristina De Amaral P. Nunes INIAP-IPIMAR Fisheries and Sea Research Institute Marine Resources Department Avenida Bras?lia 1449-006 Lisboa Portugal Tel: + 351 21 302 71 55 Fax: + 351 21 301 59 48 Email: cnunes@ipimar.pt ><<<> .....................................................................><<<> From AGrobe2555 <@t> aol.com Fri Feb 9 10:36:41 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Feb 9 10:36:58 2007 Subject: [Histonet] Re:Rabbit Smooth Muscle actin Message-ID: I've used the monoclonal anti-smooth muscle cell actin (A2547) from SIGMA with great success in rabbit tissues fixed in Histochoice. Might work for you. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From mcauliff <@t> umdnj.edu Fri Feb 9 10:43:22 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 9 10:42:35 2007 Subject: [Histonet] AFA fixative In-Reply-To: <200702091631.l19GVtPK002913@neptuno.ipimar.pt> References: <200702091631.l19GVtPK002913@neptuno.ipimar.pt> Message-ID: <45CCA4AA.3000402@umdnj.edu> Hi Christina. Well, AFA is alcohol + formaldehyde + acetic acid, none of these are good for you but neither is buffered formalin. That said, the alcohol in AFA will "carry" the other components across skin by acting as a solvent to the lipids in skin. Aqueous fixed like buffered formalin are much less likely to do this. So the good penetration properties of AFA could render it more toxic. Gloves would solve the problem. Geoff Cristina Nunes wrote: >Dear histoneters, >For several years, in the Institute where I am working, the main fixative >used for the preservation of gonads of fish was AFA, with apparently good >results. Then, at a given moment (before I arrived to the Institute), it was >decided to change from AFA to a formalin solution because of the higher >toxicity of the former. The histological results were slightly less good but >for practical reasons (most fish tissue fixations occur at sea, on board the >research vessels), it was considered to be the best solution. However, >nobody was able to explain me why AFA is much more toxic than a formalin >solution (I am sorry for my ignorance in terms of chemistry). Could anyone >clarify me on that point? >Many thanks in advance and a very nice week-end. >Regards, >Cristina Nunes. > > > >><<<> >> >> >......................................................................><<<> >Cristina De Amaral P. Nunes >INIAP-IPIMAR Fisheries and Sea Research Institute >Marine Resources Department >Avenida Bras?lia >1449-006 Lisboa >Portugal >Tel: + 351 21 302 71 55 >Fax: + 351 21 301 59 48 >Email: cnunes@ipimar.pt > > >><<<> >> >> >.....................................................................><<<> > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From cmccord <@t> ameripath.com Fri Feb 9 11:07:27 2007 From: cmccord <@t> ameripath.com (McCord, Cherie) Date: Fri Feb 9 11:07:35 2007 Subject: [Histonet] Techs & Transcriptionists Message-ID: Anyone know of a Tech who would be interested in working in the Atlanta area (Marietta, GA) to be exact. The hours are 10pm - 6:30am. Right now you would be the only person. Grossing, cutting, staining and IHC on GI biopsies. We are also in desperate need of 2 transcriptionists. Any contacts would be greatly appreciated. Denise McCord Lab Manager DermPath Diagnostics (A division of AmeriPath, Inc.) Marietta, GA 30067 cmccord@AmeriPath.com 770-612-1395 ext. 212 From POWELL_SA <@t> Mercer.edu Fri Feb 9 11:27:01 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Feb 9 11:31:46 2007 Subject: [Histonet] GSH 2007 meeting REMINDER Message-ID: <01MCXFGX2OC48WWK57@Macon2.Mercer.edu> Hi Friends, Yes I am shouting. I am again posting the program information for our meeting. The program in .PDF format can be found on our new website www.histosearch.com/gsh. Click on GSH Symposium and the program can be downloaded from there. ****Please note, the deadline for registration at Callaway Garden Mountain Creek Inn is March 1st so please make your reservations early. This is their peak season and rooms go fast, but this rate is exceptional. $119 for single, double, triple or quad. Come on down to Georgia April 13-15, 2007 to the Georgia Society for Histotechnology meeting. Shirley Powell GSH Secretary PS: Check out the facilities at Mountain Creek Inn www.callawaygardens.com. Call 1-800 225-5292 for Reservations. Please state you are attending the GSH/ Meeting when making reservations in order to get the discounted rate of $119 for single, double, triple or quad. Again, the deadline for reservations is March 1st to take advantage of this rate. From weneng2004 <@t> yahoo.com Fri Feb 9 11:49:59 2007 From: weneng2004 <@t> yahoo.com (wen eng) Date: Fri Feb 9 11:50:07 2007 Subject: [Histonet] table for microscope Message-ID: <681386.52941.qm@web55308.mail.re4.yahoo.com> Hello, We are looking for a table for my microscope. We are moving to the 5th floor and my microscope is Nikon E800. I was told I need to concern about the vibration in tall building. So I want to ask the experts here what kind of table is good enough for this. Thanks in advance. Wen ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news From mtitford <@t> aol.com Fri Feb 9 12:03:01 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Feb 9 12:03:16 2007 Subject: [Histonet] Alk. Phos. on P/S. Message-ID: <8C91A8BAC81DD98-978-A88B@WEBMAIL-DC13.sysops.aol.com> John Baker and Erik Waldorff ask about alkaline phosphatase on paraffin sections- Light years ago we used to do alkaline phosphatase on paraffin sections using a method of Gormori, modified by Lillie. There were some caveats though: 1) The fixative was cold acetone 2) The time in paraffin was was short 3) It used the old calcium phosphate method with sodium beta glycerophosphate as the substrate. Then we counterstained with methyl green. It worked just fine, and we got excellent results. EDTA decalcification is pretty benign and should not affect it much. I think the Sigma kit is for detecting alkaline phosphatase in blood smears which have been briefly fixed. I forgot my biochemistry years ago, but there also may be different alkaline phosphastases so you must select the right substrate. The procedure is on page 243 of Drury & Wallingtons "Carleton's Histological Technique" fourth edition. Oxford University Press 1967 Mike Titford Pathology USA Mobile AL USA ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From PMonfils <@t> Lifespan.org Fri Feb 9 12:14:57 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Feb 9 12:15:05 2007 Subject: [Histonet] formalin fixed mouse ears-help In-Reply-To: <5faddeb5511e5c418b5370dd72c1a740@localhost.localdomain> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C2A@LSRIEXCH1.lsmaster.lifespan.org> I had the same problem a couple of years ago. I found I could largely avoid it by processing the ears on a longer schedule, 40 hours instead of overnight (monday at quitting time through wednesday morning for example), presumably getting more thorough infiltration of the cartilage. As for those already embedded, try putting a drop of liquid dish detergent in the water bath to reduce the surface tension, and make sure the bath is not warmer than it has to be. If that doesn't help with these blocks I would melt them and put the tissue back in paraffin, preferably under vacuum, for several more hours before re-embedding. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mary Beauchamp > Sent: Thursday, February 8, 2007 2:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] formalin fixed mouse ears-help > > Hi there, > > > > I'm having problems with 10% neutral buffered formalin fixed mouse ears > embedded in paraffin. They are splitting from the cartilage as soon, and > I mean, as soon as they hit the water bath. > > > > I'm open to any suggestions, no matter how silly or simple. This is a > chronic problem and these mouse ears will continue to be submitted to me > for a long time. > > > > Please help! > > > > -Mary B > > > _____ > > I am using the free version of SPAMfighter for private users. > It has removed 78 spam emails to date. > Paying users do not have this message in their emails. > Try SPAMfighter for free now! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From contact <@t> excaliburpathology.com Fri Feb 9 12:20:51 2007 From: contact <@t> excaliburpathology.com (P Pierce) Date: Fri Feb 9 12:20:59 2007 Subject: [Histonet] table for microscope Message-ID: <159831.35180.qm@web50112.mail.yahoo.com> Eons ago the brilliant powers that were placed our TEM and SEM scopes on the 4th floor. This was fine for just viewing the specimens on the screen. However, while taking pictures, if someone walked down the hall, the SEM polaroids and TEM negatives had light and dark striations. I did have a marble table for the ultramicrotome. For your purposes, a good heavy table, not neccessarily marble, should do just fine. I assume your photomicrographs will be digital and slow exposure time not a factor. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com excaliburpathology.com From lu_ze <@t> sbcglobal.net Fri Feb 9 12:27:14 2007 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Fri Feb 9 12:27:22 2007 Subject: [Histonet] Mouse blood chemistry analysis References: <200702091759.l19Hx1g7032462@nlpi046.sbcis.sbc.com> Message-ID: <01b201c74c77$ec175600$0902a8c0@OPTIMUM2> Histonet friends, We have a Endocheck plus blood chemistry analyzer in our laboratory. In one of our project, we need to use to analyze mouse plasma samples. We have problems to get consistant results. And the reproducibility is poor with large variation. I am not sure whether someone has experience with this machine. Is there any better blood chemistry analyzer for mous plasma analysis? We have limited sample volume. Anyone want to share some experience? Thanks. =========================== Ze Lu, Ph.D. Optimum Therapeutics, LLC ----- Original Message ----- From: To: Sent: Friday, February 09, 2007 12:59 PM Subject: Histonet Digest, Vol 39, Issue 15 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. formalin fixed mouse ears-help (Gayle Callis) > 2. smooth muscle cell actin for rabbit (Galina Deyneko) > 3. 2007 Region III meeting in NC (Bly Haverland) > 4. AFA fixative (Cristina Nunes) > 5. Re:Rabbit Smooth Muscle actin (AGrobe2555@aol.com) > 6. Re: AFA fixative (Geoff McAuliffe) > 7. Techs & Transcriptionists (McCord, Cherie) > 8. GSH 2007 meeting REMINDER (Shirley Powell) > 9. table for microscope (wen eng) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 09 Feb 2007 08:20:13 -0700 > From: Gayle Callis > Subject: [Histonet] formalin fixed mouse ears-help > To: "Mary Beauchamp" , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070209080907.01b28190@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Mary, > > Since you did not say what your processing schedule is for these ears, I > suspect infiltration in paraffin is not very good so extend the time there > and use a vacuum. This still denser tissue even though it appears very > thin, and those layers can be a problem. Try at least three changes > under > vacuum. Hopefully you have vacuum and pressure on your processor, and > don't add heat to the processing solvents, these are skinny dry tissues to > begin with. > > 4. Lower the temperature of the water bath and don't over soak a trimmed > block on ice water. > > Nothing is silly or simple if you are having problems. Good luck > >>I'm having problems with 10% neutral buffered formalin fixed mouse ears >>embedded in paraffin. They are splitting from the cartilage as soon, and >>I mean, as soon as they hit the water bath. >> >>I'm open to any suggestions, no matter how silly or simple. This is a >>chronic problem and these mouse ears will continue to be submitted to me >>for a long time. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 9 Feb 2007 07:51:32 -0800 (PST) > From: Galina Deyneko > Subject: [Histonet] smooth muscle cell actin for rabbit > To: histonet@lists.utsouthwestern.edu > Message-ID: <842576.99403.qm@web33114.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Colleagues, > I am looking for primary antibody against smooth muscle cell actin in > FFPE rabbit aorta with athero lesion. I have used the mouse monoclonal > primary AB from Biocare #CM001, clone 1A4, reacts with human ,rat, rabbit > etc. with following protocol: > No pre-treatment. > Peroxidase blocking > Protein blocking > Incubation with AB 2 hours at RT > Detection with MACH 2 polymer -HRP (Biocare) > Chromogen: DAB. > AB gives non-specific staining in macrophages, and foam cells, and SMC > are stained light brown. > With mouse aorta and sinus with MOM kit this AB works very well. > The same results with monkey's coronary arteries. > Any information and protocols for both species are highly appreciated. > Sincerely. > Galina Deyneko > CardioVasccular Department > Novartis, Cambridge, MA > 617-871-7613. > > > > > > --------------------------------- > Bored stiff? Loosen up... > Download and play hundreds of games for free on Yahoo! Games. > > ------------------------------ > > Message: 3 > Date: Fri, 09 Feb 2007 11:26:49 -0500 > From: "Bly Haverland" > Subject: [Histonet] 2007 Region III meeting in NC > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > The North Carolina Society of Histopathology Technologists is pleased to > announce it will be hosting the 2007 Region III meeting this year taking > place March 22-24, 2007 in Research Triangle Park, North Carolina at the > Raleigh-Durham Airport Hilton. The Society invites anyone interested in > histology to attend. Membership is not required for participation. The > officers and volunteers of NCSHT have put together an exciting program > this > year, and they look forward to as many participants as possible. A > complete > listing of the Region III program along with registration forms is posted > on > the NSH website at www.nsh.org > > Thank you. > Bly Haverland HT, HTL > Triangle Histology Services, Inc. > corycody@msn.com > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 9 Feb 2007 16:27:09 -0000 > From: "Cristina Nunes" > Subject: [Histonet] AFA fixative > To: > Message-ID: <200702091631.l19GVtPK002913@neptuno.ipimar.pt> > Content-Type: text/plain; charset="iso-8859-1" > > Dear histoneters, > For several years, in the Institute where I am working, the main fixative > used for the preservation of gonads of fish was AFA, with apparently good > results. Then, at a given moment (before I arrived to the Institute), it > was > decided to change from AFA to a formalin solution because of the higher > toxicity of the former. The histological results were slightly less good > but > for practical reasons (most fish tissue fixations occur at sea, on board > the > research vessels), it was considered to be the best solution. However, > nobody was able to explain me why AFA is much more toxic than a formalin > solution (I am sorry for my ignorance in terms of chemistry). Could anyone > clarify me on that point? > Many thanks in advance and a very nice week-end. > Regards, > Cristina Nunes. > >><<<> > ......................................................................><<<> > Cristina De Amaral P. Nunes > INIAP-IPIMAR Fisheries and Sea Research Institute > Marine Resources Department > Avenida Bras?lia > 1449-006 Lisboa > Portugal > Tel: + 351 21 302 71 55 > Fax: + 351 21 301 59 48 > Email: cnunes@ipimar.pt >><<<> > .....................................................................><<<> > > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 9 Feb 2007 11:36:41 EST > From: AGrobe2555@aol.com > Subject: [Histonet] Re:Rabbit Smooth Muscle actin > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > I've used the monoclonal anti-smooth muscle cell actin (A2547) from SIGMA > with great success in rabbit tissues fixed in Histochoice. Might work for > you. > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > ------------------------------ > > Message: 6 > Date: Fri, 09 Feb 2007 11:43:22 -0500 > From: Geoff McAuliffe > Subject: Re: [Histonet] AFA fixative > To: Cristina Nunes > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <45CCA4AA.3000402@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > Hi Christina. > > Well, AFA is alcohol + formaldehyde + acetic acid, none of these are > good for you but neither is buffered formalin. That said, the alcohol in > AFA will "carry" the other components across skin by acting as a solvent > to the lipids in skin. Aqueous fixed like buffered formalin are much > less likely to do this. So the good penetration properties of AFA could > render it more toxic. Gloves would solve the problem. > > Geoff > > Cristina Nunes wrote: > >>Dear histoneters, >>For several years, in the Institute where I am working, the main fixative >>used for the preservation of gonads of fish was AFA, with apparently good >>results. Then, at a given moment (before I arrived to the Institute), it >>was >>decided to change from AFA to a formalin solution because of the higher >>toxicity of the former. The histological results were slightly less good >>but >>for practical reasons (most fish tissue fixations occur at sea, on board >>the >>research vessels), it was considered to be the best solution. However, >>nobody was able to explain me why AFA is much more toxic than a formalin >>solution (I am sorry for my ignorance in terms of chemistry). Could anyone >>clarify me on that point? >>Many thanks in advance and a very nice week-end. >>Regards, >>Cristina Nunes. >> >> >> >>><<<> >>> >>> >>......................................................................><<<> >>Cristina De Amaral P. Nunes >>INIAP-IPIMAR Fisheries and Sea Research Institute >>Marine Resources Department >>Avenida Bras?lia >>1449-006 Lisboa >>Portugal >>Tel: + 351 21 302 71 55 >>Fax: + 351 21 301 59 48 >>Email: cnunes@ipimar.pt >> >> >>><<<> >>> >>> >>.....................................................................><<<> >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > > ------------------------------ > > Message: 7 > Date: Fri, 9 Feb 2007 11:07:27 -0600 > From: "McCord, Cherie" > Subject: [Histonet] Techs & Transcriptionists > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Anyone know of a Tech who would be interested in working in the Atlanta > area (Marietta, GA) to be exact. The hours are 10pm - 6:30am. Right > now you would be the only person. Grossing, cutting, staining and IHC > on GI biopsies. We are also in desperate need of 2 transcriptionists. > Any contacts would be greatly appreciated. > > > > Denise McCord > > Lab Manager > > DermPath Diagnostics > > (A division of AmeriPath, Inc.) > > Marietta, GA 30067 > > cmccord@AmeriPath.com > > 770-612-1395 ext. 212 > > > > > > ------------------------------ > > Message: 8 > Date: Fri, 09 Feb 2007 12:27:01 -0500 > From: Shirley Powell > Subject: [Histonet] GSH 2007 meeting REMINDER > To: histonet@lists.utsouthwestern.edu > Message-ID: <01MCXFGX2OC48WWK57@Macon2.Mercer.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi Friends, > > Yes I am shouting. I am again posting the program information for our > meeting. The program in .PDF format can be found on our new website > www.histosearch.com/gsh. Click on GSH Symposium and the program can be > downloaded from there. > > ****Please note, the deadline for registration at Callaway Garden Mountain > Creek Inn is March 1st so please make your reservations early. This is > their peak season and rooms go fast, but this rate is exceptional. $119 > for > single, double, triple or quad. > > Come on down to Georgia April 13-15, 2007 to the Georgia Society for > Histotechnology meeting. > > Shirley Powell > GSH Secretary > > PS: Check out the facilities at Mountain Creek Inn > www.callawaygardens.com. > Call 1-800 225-5292 for Reservations. Please state you are attending the > GSH/ Meeting when making reservations in order to get the discounted rate > of > $119 for single, double, triple or quad. > > Again, the deadline for reservations is March 1st to take advantage of > this > rate. > > > > > ------------------------------ > > Message: 9 > Date: Fri, 9 Feb 2007 09:49:59 -0800 (PST) > From: wen eng > Subject: [Histonet] table for microscope > To: histonet > Message-ID: <681386.52941.qm@web55308.mail.re4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello, > > We are looking for a table for my microscope. We are moving to the 5th > floor and my microscope is Nikon E800. I was told I need to concern about > the vibration in tall building. So I want to ask the experts here what > kind of table is good enough for this. > > Thanks in advance. > Wen > > > > ____________________________________________________________________________________ > 8:00? 8:25? 8:40? Find a flick in no time > with the Yahoo! Search movie showtime shortcut. > http://tools.search.yahoo.com/shortcuts/#news > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 39, Issue 15 > **************************************** From kbowden <@t> ucsd.edu Fri Feb 9 12:40:27 2007 From: kbowden <@t> ucsd.edu (kbowden) Date: Fri Feb 9 12:40:48 2007 Subject: [Histonet] table for microscope In-Reply-To: <681386.52941.qm@web55308.mail.re4.yahoo.com> References: <681386.52941.qm@web55308.mail.re4.yahoo.com> Message-ID: <45CCC01B.8030804@ucsd.edu> We had to have a five inch thick marble table. Karen Bowden Staff Research Associate II University of CA, San Diego 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 Voice 858-534-5304 Fax kbowden@ucsd.edu CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER ANDDELETE THE MATERIAL FROM ANY COMPUTER. wen eng wrote: > Hello, > > We are looking for a table for my microscope. We are moving to the 5th floor and my microscope is Nikon E800. I was told I need to concern about the vibration in tall building. So I want to ask the experts here what kind of table is good enough for this. > > Thanks in advance. > Wen > > > > ____________________________________________________________________________________ > 8:00? 8:25? 8:40? Find a flick in no time > with the Yahoo! Search movie showtime shortcut. > http://tools.search.yahoo.com/shortcuts/#news > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From settembr <@t> umdnj.edu Fri Feb 9 12:54:30 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Feb 9 12:55:29 2007 Subject: [Histonet] Fli-1 vendors? Message-ID: I am looking for IVD Fli-1 to use on formalin fixed paraffin embeded human tissue. Is there such an animal? I have tried Santa Cruz (not IVD) Thank you, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From caron_fournier <@t> yahoo.ca Fri Feb 9 13:17:08 2007 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Fri Feb 9 13:17:21 2007 Subject: [Histonet] Re: PMMA removal Message-ID: <275776.31186.qm@web35409.mail.mud.yahoo.com> I was wondering if anyone has a method for removing PMMA from bone without harming the bone. Thank you for your help. Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ----- __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From robert.kan <@t> us.army.mil Fri Feb 9 13:20:23 2007 From: robert.kan <@t> us.army.mil (Kan, Robert K Dr USAMRICD) Date: Fri Feb 9 13:20:59 2007 Subject: [Histonet] RE: Histonet Digest, Vol 39, Issue 15 In-Reply-To: <5j0n9n$6mu71i@mxoutdr1.us.army.mil> References: <5j0n9n$6mu71i@mxoutdr1.us.army.mil> Message-ID: Hello All, We are conducting a study to evaluate the amount of exudate or degree of pulmonary edema in the rat. What is the best fixative for fixing the exudate in the alveolar space without compromising immunohistochemical procedures. Any help you can give me would be appreciated. Robert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 09, 2007 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 39, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. formalin fixed mouse ears-help (Gayle Callis) 2. smooth muscle cell actin for rabbit (Galina Deyneko) 3. 2007 Region III meeting in NC (Bly Haverland) 4. AFA fixative (Cristina Nunes) 5. Re:Rabbit Smooth Muscle actin (AGrobe2555@aol.com) 6. Re: AFA fixative (Geoff McAuliffe) 7. Techs & Transcriptionists (McCord, Cherie) 8. GSH 2007 meeting REMINDER (Shirley Powell) 9. table for microscope (wen eng) ---------------------------------------------------------------------- Message: 1 Date: Fri, 09 Feb 2007 08:20:13 -0700 From: Gayle Callis Subject: [Histonet] formalin fixed mouse ears-help To: "Mary Beauchamp" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070209080907.01b28190@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Mary, Since you did not say what your processing schedule is for these ears, I suspect infiltration in paraffin is not very good so extend the time there and use a vacuum. This still denser tissue even though it appears very thin, and those layers can be a problem. Try at least three changes under vacuum. Hopefully you have vacuum and pressure on your processor, and don't add heat to the processing solvents, these are skinny dry tissues to begin with. 4. Lower the temperature of the water bath and don't over soak a trimmed block on ice water. Nothing is silly or simple if you are having problems. Good luck >I'm having problems with 10% neutral buffered formalin fixed mouse ears >embedded in paraffin. They are splitting from the cartilage as soon, >and I mean, as soon as they hit the water bath. > >I'm open to any suggestions, no matter how silly or simple. This is a >chronic problem and these mouse ears will continue to be submitted to >me for a long time. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 2 Date: Fri, 9 Feb 2007 07:51:32 -0800 (PST) From: Galina Deyneko Subject: [Histonet] smooth muscle cell actin for rabbit To: histonet@lists.utsouthwestern.edu Message-ID: <842576.99403.qm@web33114.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Colleagues, I am looking for primary antibody against smooth muscle cell actin in FFPE rabbit aorta with athero lesion. I have used the mouse monoclonal primary AB from Biocare #CM001, clone 1A4, reacts with human ,rat, rabbit etc. with following protocol: No pre-treatment. Peroxidase blocking Protein blocking Incubation with AB 2 hours at RT Detection with MACH 2 polymer -HRP (Biocare) Chromogen: DAB. AB gives non-specific staining in macrophages, and foam cells, and SMC are stained light brown. With mouse aorta and sinus with MOM kit this AB works very well. The same results with monkey's coronary arteries. Any information and protocols for both species are highly appreciated. Sincerely. Galina Deyneko CardioVasccular Department Novartis, Cambridge, MA 617-871-7613. --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. ------------------------------ Message: 3 Date: Fri, 09 Feb 2007 11:26:49 -0500 From: "Bly Haverland" Subject: [Histonet] 2007 Region III meeting in NC To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed The North Carolina Society of Histopathology Technologists is pleased to announce it will be hosting the 2007 Region III meeting this year taking place March 22-24, 2007 in Research Triangle Park, North Carolina at the Raleigh-Durham Airport Hilton. The Society invites anyone interested in histology to attend. Membership is not required for participation. The officers and volunteers of NCSHT have put together an exciting program this year, and they look forward to as many participants as possible. A complete listing of the Region III program along with registration forms is posted on the NSH website at www.nsh.org Thank you. Bly Haverland HT, HTL Triangle Histology Services, Inc. corycody@msn.com ------------------------------ Message: 4 Date: Fri, 9 Feb 2007 16:27:09 -0000 From: "Cristina Nunes" Subject: [Histonet] AFA fixative To: Message-ID: <200702091631.l19GVtPK002913@neptuno.ipimar.pt> Content-Type: text/plain; charset="iso-8859-1" Dear histoneters, For several years, in the Institute where I am working, the main fixative used for the preservation of gonads of fish was AFA, with apparently good results. Then, at a given moment (before I arrived to the Institute), it was decided to change from AFA to a formalin solution because of the higher toxicity of the former. The histological results were slightly less good but for practical reasons (most fish tissue fixations occur at sea, on board the research vessels), it was considered to be the best solution. However, nobody was able to explain me why AFA is much more toxic than a formalin solution (I am sorry for my ignorance in terms of chemistry). Could anyone clarify me on that point? Many thanks in advance and a very nice week-end. Regards, Cristina Nunes. ><<<> ......................................................................><<<> Cristina De Amaral P. Nunes INIAP-IPIMAR Fisheries and Sea Research Institute Marine Resources Department Avenida Bras?lia 1449-006 Lisboa Portugal Tel: + 351 21 302 71 55 Fax: + 351 21 301 59 48 Email: cnunes@ipimar.pt ><<<> .....................................................................><<<> ------------------------------ Message: 5 Date: Fri, 9 Feb 2007 11:36:41 EST From: AGrobe2555@aol.com Subject: [Histonet] Re:Rabbit Smooth Muscle actin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I've used the monoclonal anti-smooth muscle cell actin (A2547) from SIGMA with great success in rabbit tissues fixed in Histochoice. Might work for you. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ------------------------------ Message: 6 Date: Fri, 09 Feb 2007 11:43:22 -0500 From: Geoff McAuliffe Subject: Re: [Histonet] AFA fixative To: Cristina Nunes Cc: histonet@lists.utsouthwestern.edu Message-ID: <45CCA4AA.3000402@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Hi Christina. Well, AFA is alcohol + formaldehyde + acetic acid, none of these are good for you but neither is buffered formalin. That said, the alcohol in AFA will "carry" the other components across skin by acting as a solvent to the lipids in skin. Aqueous fixed like buffered formalin are much less likely to do this. So the good penetration properties of AFA could render it more toxic. Gloves would solve the problem. Geoff Cristina Nunes wrote: >Dear histoneters, >For several years, in the Institute where I am working, the main >fixative used for the preservation of gonads of fish was AFA, with >apparently good results. Then, at a given moment (before I arrived to >the Institute), it was decided to change from AFA to a formalin >solution because of the higher toxicity of the former. The histological >results were slightly less good but for practical reasons (most fish >tissue fixations occur at sea, on board the research vessels), it was >considered to be the best solution. However, nobody was able to explain >me why AFA is much more toxic than a formalin solution (I am sorry for >my ignorance in terms of chemistry). Could anyone clarify me on that point? >Many thanks in advance and a very nice week-end. >Regards, >Cristina Nunes. > > > >><<<> >> >> >......................................................................> ><<<> >Cristina De Amaral P. Nunes >INIAP-IPIMAR Fisheries and Sea Research Institute Marine Resources >Department Avenida Bras?lia >1449-006 Lisboa >Portugal >Tel: + 351 21 302 71 55 >Fax: + 351 21 301 59 48 >Email: cnunes@ipimar.pt > > >><<<> >> >> >.....................................................................>< ><<> > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 7 Date: Fri, 9 Feb 2007 11:07:27 -0600 From: "McCord, Cherie" Subject: [Histonet] Techs & Transcriptionists To: Message-ID: Content-Type: text/plain; charset="us-ascii" Anyone know of a Tech who would be interested in working in the Atlanta area (Marietta, GA) to be exact. The hours are 10pm - 6:30am. Right now you would be the only person. Grossing, cutting, staining and IHC on GI biopsies. We are also in desperate need of 2 transcriptionists. Any contacts would be greatly appreciated. Denise McCord Lab Manager DermPath Diagnostics (A division of AmeriPath, Inc.) Marietta, GA 30067 cmccord@AmeriPath.com 770-612-1395 ext. 212 ------------------------------ Message: 8 Date: Fri, 09 Feb 2007 12:27:01 -0500 From: Shirley Powell Subject: [Histonet] GSH 2007 meeting REMINDER To: histonet@lists.utsouthwestern.edu Message-ID: <01MCXFGX2OC48WWK57@Macon2.Mercer.edu> Content-Type: text/plain; charset=US-ASCII Hi Friends, Yes I am shouting. I am again posting the program information for our meeting. The program in .PDF format can be found on our new website www.histosearch.com/gsh. Click on GSH Symposium and the program can be downloaded from there. ****Please note, the deadline for registration at Callaway Garden Mountain Creek Inn is March 1st so please make your reservations early. This is their peak season and rooms go fast, but this rate is exceptional. $119 for single, double, triple or quad. Come on down to Georgia April 13-15, 2007 to the Georgia Society for Histotechnology meeting. Shirley Powell GSH Secretary PS: Check out the facilities at Mountain Creek Inn www.callawaygardens.com. Call 1-800 225-5292 for Reservations. Please state you are attending the GSH/ Meeting when making reservations in order to get the discounted rate of $119 for single, double, triple or quad. Again, the deadline for reservations is March 1st to take advantage of this rate. ------------------------------ Message: 9 Date: Fri, 9 Feb 2007 09:49:59 -0800 (PST) From: wen eng Subject: [Histonet] table for microscope To: histonet Message-ID: <681386.52941.qm@web55308.mail.re4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, We are looking for a table for my microscope. We are moving to the 5th floor and my microscope is Nikon E800. I was told I need to concern about the vibration in tall building. So I want to ask the experts here what kind of table is good enough for this. Thanks in advance. Wen ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 15 **************************************** From lu_ze <@t> sbcglobal.net Fri Feb 9 13:38:54 2007 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Fri Feb 9 13:38:59 2007 Subject: [Histonet] Mouse blood chemistry analysis Message-ID: <01eb01c74c81$eeb574f0$0902a8c0@OPTIMUM2> Histonet friends, We have a Endocheck plus blood chemistry analyzer in our laboratory. In one of our project, we need to use to analyze mouse plasma samples. We have problems to get consistant results. And the reproducibility is poor with large variation. I am not sure whether someone has experience with this machine. Is there any better blood chemistry analyzer for mous plasma analysis? We have limited sample volume. Anyone want to share some experience? Thanks. =========================== Ze Lu, Ph.D. Optimum Therapeutics, LLC From mauger <@t> email.chop.edu Fri Feb 9 13:39:47 2007 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Feb 9 13:40:23 2007 Subject: [Histonet] Fli-1 vendors? Message-ID: Dana, I am having success with one from BD Biosciences, but it's not IVD. Cat#554266, cloneG146-222. It does work on FFPE with antigen retrieval(EDTA pH8) Jo Mauger From gcallis <@t> montana.edu Fri Feb 9 13:44:00 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Feb 9 13:44:12 2007 Subject: [Histonet] Re: PMMA removal In-Reply-To: <275776.31186.qm@web35409.mail.mud.yahoo.com> References: <275776.31186.qm@web35409.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20070209123638.01b16e58@gemini.msu.montana.edu> Caron, Question: Are you asking about large bone samples or thin, 5 um microtomed bone sections? It is really not possible to totally remove infiltrated and then polymerized PMMA from larger bone samples or slabs once it is inside the boney structures/components. However, one can remove rapidly polymerized bubbled PMMA from outer surfaces of PMMA infiltrated and then polymerized bone sample without hurting the bone. This required grinding away every extra fragment of PMMA down to the bone, then immersing it into either pure monomer. Removing PMMA from a microtomed section mounted on a slide is done by immersion into hot (60C) xylene, several changes. Other solvents can be used for this purpose, and is a technic Neil Hand used before doing immunohistochemical staining on PMMA embedded tissues. At 12:17 PM 2/9/2007, you wrote: >I was wondering if anyone has a method for removing PMMA from bone without >harming the bone. Thank you for your help. > >Caron Fournier, BSc, R.T. > > >Department of Orthopaedics, > > >Division of Orthopaedic Engineering Research, > > >U.B.C. > > > > > > >----- > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From PMonfils <@t> Lifespan.org Fri Feb 9 13:59:40 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Feb 9 13:59:47 2007 Subject: [Histonet] Re: PMMA removal In-Reply-To: <275776.31186.qm@web35409.mail.mud.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C2E@LSRIEXCH1.lsmaster.lifespan.org> There are a number of solvents that will dissolve PMMA without harming the tissue, including toluene, xylene, chloroform, dichloromethane and ethyl acetate. Some work faster than others, and of course they work faster warm than cold, but keep in mind that they are all flammable. My preference is 2-methoxyethyl acetate, which works relatively rapidly. I use it both for deplasticizing sections before staining, and occasionally for dissolving whole blocks. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of caron fournier > Sent: Friday, February 9, 2007 11:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: PMMA removal > > I was wondering if anyone has a method for removing PMMA from bone without harming the bone. Thank you for your help. > > Caron Fournier, BSc, R.T. > > > Department of Orthopaedics, > > > Division of Orthopaedic Engineering Research, > > > U.B.C. > > > > > > > ----- > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TJasper <@t> smdc.org Fri Feb 9 14:00:05 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Feb 9 14:00:20 2007 Subject: [Histonet] Latest Pay Range Survey Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A510@SCREECH.ntcampus.smdc.org> Hello All, Would someone be able to direct me to a recent pay range survey? This would be for the US and I am specifically interested in compensation rates for Moh's Technicians. I did search about on the ASCP website, but to no avail. I also know this has been asked before and I do appreciate someone taking the time to provide assistance to me. Thank you in advance, Thomas Jasper Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org From tbullard1231 <@t> yahoo.com Fri Feb 9 15:13:10 2007 From: tbullard1231 <@t> yahoo.com (Tara Bullard) Date: Fri Feb 9 15:13:19 2007 Subject: [Histonet] antibody question Message-ID: <71137.77052.qm@web50915.mail.yahoo.com> Hi All, When choosing an antibody for immunofluorescence which is better full length, N-term or C-term? Thanks So Much! Tara --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From mcauliff <@t> umdnj.edu Fri Feb 9 15:44:15 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 9 15:43:31 2007 Subject: [Histonet] carbowax/polyester wax Message-ID: <45CCEB2F.6030901@umdnj.edu> Greetings all: We want to try embedding zebrafish in polyester wax/carbowax. Any thoughts, suggestions, protocols, references, vendors, etc. would be welcome. Thanks! Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From akbitting <@t> geisinger.edu Fri Feb 9 15:53:34 2007 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Feb 9 15:53:53 2007 Subject: [Histonet] Paraform cassettes Message-ID: <45CCA70E020000C900005968@GHSGWIANW5V.GEISINGER.EDU> Has anyone had experience with laser printing on the Paraform cassettes from Sakura? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Eric <@t> ategra.com Fri Feb 9 18:26:33 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Feb 9 19:53:32 2007 Subject: [Histonet] Histology opportunities in your area? (Update 2/9/07) Message-ID: Hi - Histonetters - I have both permanent and full time (permanent) jobs for HistoTechs throughout the US. Are you still working as a HistoTech at ? These permanent and temporary HistoTech jobs are being filled quickly, don't miss out on your dream job- call today! Permanent Histology Jobs: (Updated Feb 9 2007) ------------------------------------------------------- New Permanent Jobs Listed below ------------------------------------------------------- 1. Central Ohio- Senior Pathology Assistant - AAPA is Required 2. Central Ohio - A.P Manager - ASCP is required 3. Southern Virginia- Histotech - Bench - Great Location, Great pay- call now! 4.Florida( Tampa Bay area) Histotech- bench- perm 5.Massachusetts (Greater Boston Area) Histotech- Bench - perm 6.Massachusetts ( Greater Boston Area) Histotech Supervisor 7.Northern Ohio - Histotech - Bench- perm ------------------------------------ Older Jobs I have available ---------------------------------- 01. Central Virginia- Histotech- perm 02. Southern California- Histotech- perm 03. Central Florida - Histotech with some MOHS experience - perm - Hot 04. Southeast South Dakota- Histotech - Must have ties to South Dakota- perm- Hot! 05. Hot! Eastern, Massachusetts Seeking Histotechs of all experience levels, Great Pay, Location And Benefits- Call Today - 15% Pay Raise Guaranteed! 06.NEWJOB! Southeast Florida- Histotech - perm - (Need Florida License) 07.Ohio (Southern) - perm - Bench Histotech ( 2 openings) 08.Ohio (Northern) perm- Bench 09.Ohio (Central) perm- Bench 10.Central Florida -perm- Histotech (Need Florida License) 11. Florida (Tampa Bay area) 12. Florida, West Coast - both temp & perm openings- Bench Histotech -Very-Hot 13. New York ( Syracuse area) - Bench Histotech- perm 14. New York City (Long Island) - Bench- perm 15. Las Vegas - Bench Histotech- perm 16. Wisconsin- Histology Supervisor- BrandNew -Hot Hot Hot 17. Central-Illinois-Bench-Dermpath- BrandNew -Hot 18. Northern California- Bench- Routine Histology- BrandNew -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From jhnspam <@t> aol.com Fri Feb 9 21:51:19 2007 From: jhnspam <@t> aol.com (jhnspam@aol.com) Date: Fri Feb 9 21:51:35 2007 Subject: [Histonet] Help with fat tissue from mice Message-ID: I am cutting frozen sections of fat from mice. Since I turned the temp to -30 I have had great results getting the tissue to section. The problem I am having is that it is washing off the slide once the investigator starts doing her immuno's. I am using positively charged slides. Does anyone have any ideas to help keep the tissue to stay on the slide? Thanks, Pam From jhnspam <@t> aol.com Fri Feb 9 21:57:36 2007 From: jhnspam <@t> aol.com (jhnspam@aol.com) Date: Fri Feb 9 21:57:49 2007 Subject: [Histonet] Animal Tissue Message-ID: I need to get some input on cutting animal tissue. I have always worked in a clinical setting and have recently moved into a research lab. I find that the tissue is very brittle and needs to be iced for an extremely amount of time. Can any of you animal cutting histo techs please give me some advice on what type of paraffin you are using and what processing schedule you are using. I have recently changed to Richard Allen type 9 paraffin and it seems to have helped some. I would appreciate any advice you can give me. Thanks, Pam From rjbuesa <@t> yahoo.com Sat Feb 10 07:30:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 10 07:31:00 2007 Subject: [Histonet] Mouse blood chemistry analysis In-Reply-To: <01b201c74c77$ec175600$0902a8c0@OPTIMUM2> Message-ID: <20070210133053.71548.qmail@web61216.mail.yahoo.com> My cat's veterinary has a blood analyzer that seems to work fine. Why don't you ask in a veterinary school? For sure they will know about reliable analyzers. Ren? J. Ze Lu wrote: Histonet friends, We have a Endocheck plus blood chemistry analyzer in our laboratory. In one of our project, we need to use to analyze mouse plasma samples. We have problems to get consistant results. And the reproducibility is poor with large variation. I am not sure whether someone has experience with this machine. Is there any better blood chemistry analyzer for mous plasma analysis? We have limited sample volume. Anyone want to share some experience? Thanks. =========================== Ze Lu, Ph.D. Optimum Therapeutics, LLC ----- Original Message ----- From: To: Sent: Friday, February 09, 2007 12:59 PM Subject: Histonet Digest, Vol 39, Issue 15 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. formalin fixed mouse ears-help (Gayle Callis) > 2. smooth muscle cell actin for rabbit (Galina Deyneko) > 3. 2007 Region III meeting in NC (Bly Haverland) > 4. AFA fixative (Cristina Nunes) > 5. Re:Rabbit Smooth Muscle actin (AGrobe2555@aol.com) > 6. Re: AFA fixative (Geoff McAuliffe) > 7. Techs & Transcriptionists (McCord, Cherie) > 8. GSH 2007 meeting REMINDER (Shirley Powell) > 9. table for microscope (wen eng) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 09 Feb 2007 08:20:13 -0700 > From: Gayle Callis > Subject: [Histonet] formalin fixed mouse ears-help > To: "Mary Beauchamp" , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070209080907.01b28190@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Mary, > > Since you did not say what your processing schedule is for these ears, I > suspect infiltration in paraffin is not very good so extend the time there > and use a vacuum. This still denser tissue even though it appears very > thin, and those layers can be a problem. Try at least three changes > under > vacuum. Hopefully you have vacuum and pressure on your processor, and > don't add heat to the processing solvents, these are skinny dry tissues to > begin with. > > 4. Lower the temperature of the water bath and don't over soak a trimmed > block on ice water. > > Nothing is silly or simple if you are having problems. Good luck > >>I'm having problems with 10% neutral buffered formalin fixed mouse ears >>embedded in paraffin. They are splitting from the cartilage as soon, and >>I mean, as soon as they hit the water bath. >> >>I'm open to any suggestions, no matter how silly or simple. This is a >>chronic problem and these mouse ears will continue to be submitted to me >>for a long time. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 9 Feb 2007 07:51:32 -0800 (PST) > From: Galina Deyneko > Subject: [Histonet] smooth muscle cell actin for rabbit > To: histonet@lists.utsouthwestern.edu > Message-ID: <842576.99403.qm@web33114.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Colleagues, > I am looking for primary antibody against smooth muscle cell actin in > FFPE rabbit aorta with athero lesion. I have used the mouse monoclonal > primary AB from Biocare #CM001, clone 1A4, reacts with human ,rat, rabbit > etc. with following protocol: > No pre-treatment. > Peroxidase blocking > Protein blocking > Incubation with AB 2 hours at RT > Detection with MACH 2 polymer -HRP (Biocare) > Chromogen: DAB. > AB gives non-specific staining in macrophages, and foam cells, and SMC > are stained light brown. > With mouse aorta and sinus with MOM kit this AB works very well. > The same results with monkey's coronary arteries. > Any information and protocols for both species are highly appreciated. > Sincerely. > Galina Deyneko > CardioVasccular Department > Novartis, Cambridge, MA > 617-871-7613. > > > > > > --------------------------------- > Bored stiff? Loosen up... > Download and play hundreds of games for free on Yahoo! Games. > > ------------------------------ > > Message: 3 > Date: Fri, 09 Feb 2007 11:26:49 -0500 > From: "Bly Haverland" > Subject: [Histonet] 2007 Region III meeting in NC > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > The North Carolina Society of Histopathology Technologists is pleased to > announce it will be hosting the 2007 Region III meeting this year taking > place March 22-24, 2007 in Research Triangle Park, North Carolina at the > Raleigh-Durham Airport Hilton. The Society invites anyone interested in > histology to attend. Membership is not required for participation. The > officers and volunteers of NCSHT have put together an exciting program > this > year, and they look forward to as many participants as possible. A > complete > listing of the Region III program along with registration forms is posted > on > the NSH website at www.nsh.org > > Thank you. > Bly Haverland HT, HTL > Triangle Histology Services, Inc. > corycody@msn.com > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 9 Feb 2007 16:27:09 -0000 > From: "Cristina Nunes" > Subject: [Histonet] AFA fixative > To: > Message-ID: <200702091631.l19GVtPK002913@neptuno.ipimar.pt> > Content-Type: text/plain; charset="iso-8859-1" > > Dear histoneters, > For several years, in the Institute where I am working, the main fixative > used for the preservation of gonads of fish was AFA, with apparently good > results. Then, at a given moment (before I arrived to the Institute), it > was > decided to change from AFA to a formalin solution because of the higher > toxicity of the former. The histological results were slightly less good > but > for practical reasons (most fish tissue fixations occur at sea, on board > the > research vessels), it was considered to be the best solution. However, > nobody was able to explain me why AFA is much more toxic than a formalin > solution (I am sorry for my ignorance in terms of chemistry). Could anyone > clarify me on that point? > Many thanks in advance and a very nice week-end. > Regards, > Cristina Nunes. > >><<<> > ......................................................................><<<> > Cristina De Amaral P. Nunes > INIAP-IPIMAR Fisheries and Sea Research Institute > Marine Resources Department > Avenida Bras?lia > 1449-006 Lisboa > Portugal > Tel: + 351 21 302 71 55 > Fax: + 351 21 301 59 48 > Email: cnunes@ipimar.pt >><<<> > .....................................................................><<<> > > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 9 Feb 2007 11:36:41 EST > From: AGrobe2555@aol.com > Subject: [Histonet] Re:Rabbit Smooth Muscle actin > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > I've used the monoclonal anti-smooth muscle cell actin (A2547) from SIGMA > with great success in rabbit tissues fixed in Histochoice. Might work for > you. > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > ------------------------------ > > Message: 6 > Date: Fri, 09 Feb 2007 11:43:22 -0500 > From: Geoff McAuliffe > Subject: Re: [Histonet] AFA fixative > To: Cristina Nunes > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <45CCA4AA.3000402@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > Hi Christina. > > Well, AFA is alcohol + formaldehyde + acetic acid, none of these are > good for you but neither is buffered formalin. That said, the alcohol in > AFA will "carry" the other components across skin by acting as a solvent > to the lipids in skin. Aqueous fixed like buffered formalin are much > less likely to do this. So the good penetration properties of AFA could > render it more toxic. Gloves would solve the problem. > > Geoff > > Cristina Nunes wrote: > >>Dear histoneters, >>For several years, in the Institute where I am working, the main fixative >>used for the preservation of gonads of fish was AFA, with apparently good >>results. Then, at a given moment (before I arrived to the Institute), it >>was >>decided to change from AFA to a formalin solution because of the higher >>toxicity of the former. The histological results were slightly less good >>but >>for practical reasons (most fish tissue fixations occur at sea, on board >>the >>research vessels), it was considered to be the best solution. However, >>nobody was able to explain me why AFA is much more toxic than a formalin >>solution (I am sorry for my ignorance in terms of chemistry). Could anyone >>clarify me on that point? >>Many thanks in advance and a very nice week-end. >>Regards, >>Cristina Nunes. >> >> >> >>><<<> >>> >>> >>......................................................................><<<> >>Cristina De Amaral P. Nunes >>INIAP-IPIMAR Fisheries and Sea Research Institute >>Marine Resources Department >>Avenida Bras?lia >>1449-006 Lisboa >>Portugal >>Tel: + 351 21 302 71 55 >>Fax: + 351 21 301 59 48 >>Email: cnunes@ipimar.pt >> >> >>><<<> >>> >>> >>.....................................................................><<<> >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > > ------------------------------ > > Message: 7 > Date: Fri, 9 Feb 2007 11:07:27 -0600 > From: "McCord, Cherie" > Subject: [Histonet] Techs & Transcriptionists > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Anyone know of a Tech who would be interested in working in the Atlanta > area (Marietta, GA) to be exact. The hours are 10pm - 6:30am. Right > now you would be the only person. Grossing, cutting, staining and IHC > on GI biopsies. We are also in desperate need of 2 transcriptionists. > Any contacts would be greatly appreciated. > > > > Denise McCord > > Lab Manager > > DermPath Diagnostics > > (A division of AmeriPath, Inc.) > > Marietta, GA 30067 > > cmccord@AmeriPath.com > > 770-612-1395 ext. 212 > > > > > > ------------------------------ > > Message: 8 > Date: Fri, 09 Feb 2007 12:27:01 -0500 > From: Shirley Powell > Subject: [Histonet] GSH 2007 meeting REMINDER > To: histonet@lists.utsouthwestern.edu > Message-ID: <01MCXFGX2OC48WWK57@Macon2.Mercer.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi Friends, > > Yes I am shouting. I am again posting the program information for our > meeting. The program in .PDF format can be found on our new website > www.histosearch.com/gsh. Click on GSH Symposium and the program can be > downloaded from there. > > ****Please note, the deadline for registration at Callaway Garden Mountain > Creek Inn is March 1st so please make your reservations early. This is > their peak season and rooms go fast, but this rate is exceptional. $119 > for > single, double, triple or quad. > > Come on down to Georgia April 13-15, 2007 to the Georgia Society for > Histotechnology meeting. > > Shirley Powell > GSH Secretary > > PS: Check out the facilities at Mountain Creek Inn > www.callawaygardens.com. > Call 1-800 225-5292 for Reservations. Please state you are attending the > GSH/ Meeting when making reservations in order to get the discounted rate > of > $119 for single, double, triple or quad. > > Again, the deadline for reservations is March 1st to take advantage of > this > rate. > > > > > ------------------------------ > > Message: 9 > Date: Fri, 9 Feb 2007 09:49:59 -0800 (PST) > From: wen eng > Subject: [Histonet] table for microscope > To: histonet > Message-ID: <681386.52941.qm@web55308.mail.re4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello, > > We are looking for a table for my microscope. We are moving to the 5th > floor and my microscope is Nikon E800. I was told I need to concern about > the vibration in tall building. So I want to ask the experts here what > kind of table is good enough for this. > > Thanks in advance. > Wen > > > > ____________________________________________________________________________________ > 8:00? 8:25? 8:40? Find a flick in no time > with the Yahoo! Search movie showtime shortcut. > http://tools.search.yahoo.com/shortcuts/#news > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 39, Issue 15 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From lpwenk <@t> sbcglobal.net Sat Feb 10 07:55:14 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Feb 10 07:55:48 2007 Subject: [Histonet] Latest Pay Range Survey In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A510@SCREECH.ntcampus.smdc.org> Message-ID: <000001c74d1b$17fdc030$5e0e2e4b@HPPav2> The ASCP surveys are published about 1 year after the survey. So the latest survey was published in August 2006, from statistics gathered in 2005. http://www.ascp.org/Certification/ForProgramDirectors/research/documents/wva c2005.pdf Peggy A. Wenk, HTL(ASCP) William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: Friday, February 09, 2007 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Latest Pay Range Survey Hello All, Would someone be able to direct me to a recent pay range survey? This would be for the US and I am specifically interested in compensation rates for Moh's Technicians. I did search about on the ASCP website, but to no avail. I also know this has been asked before and I do appreciate someone taking the time to provide assistance to me. Thank you in advance, Thomas Jasper Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Sat Feb 10 09:18:05 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sat Feb 10 09:18:19 2007 Subject: [Histonet] Iron Control Message-ID: Hello All, We are almost out of Iron Control. We have been using Lung with a chronic hemosiderosis condition. The Docs say that it could be a while before we find a suitable control. Could anyone tell me what they are using that might be easier to find? Thanks in advance Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA From RSRICHMOND <@t> aol.com Sat Feb 10 09:33:05 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Feb 10 09:33:18 2007 Subject: [Histonet] Re: AFA fixative Message-ID: Cristina De Amaral P. Nunes at the INIAP-IPIMAR Fisheries and Sea Research Institute Marine In Lisbon, Portugal asks: >>For several years, in the Institute where I am working, the main fixative used for the preservation of gonads of fish was AFA, with apparently good results. Then, at a given moment (before I arrived to the Institute), it was decided to change from AFA to a formalin solution because of the higher toxicity of the former. The histological results were slightly less good but for practical reasons (most fish tissue fixations occur at sea, on board the research vessels), it was considered to be the best solution.<< and Geoff McAuliffe notes that >>AFA is alcohol + formaldehyde + acetic acid.< < Assuming that AFA is indeed similar to Davidson's fixative, it would probably be a better fixative for gonadal tissue. I think there are two reasons not to use it for tissue fixation at sea, partly its toxicity (including possible fire hazard, depending on its composition), but also because the fixative will damage tissue (with loss of nuclear basophilia) if exposure to the fixative is continued much past 24 hours. I think that phosphate buffered formalin (neutral buffered formalin) would probably be the fixative of choice. AFA would be more toxic than neutral buffered formalin because of its higher formaldehyde content, its acidity, and the presence of alcohol, to say nothing of its unpleasant and penetrating ("airplane dope") odor. Pathologists use it to locate lymph nodes in fatty tissue, and it's unpleasant to handle even with good ventilation and high quality gloves - it degrades gloves (including some nitrile rubber gloves) rapidly Bob Richmond Samurai Pathologist Knoxville, Tennessee USA From rjbuesa <@t> yahoo.com Sat Feb 10 10:38:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 10 10:38:47 2007 Subject: [Histonet] Iron Control In-Reply-To: Message-ID: <331893.41496.qm@web61219.mail.yahoo.com> Positive bone marrow biopsies and liver are easier to find. Ren? J. sheila adey wrote: Hello All, We are almost out of Iron Control. We have been using Lung with a chronic hemosiderosis condition. The Docs say that it could be a while before we find a suitable control. Could anyone tell me what they are using that might be easier to find? Thanks in advance Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://discoverspaces.live.com/?loc=en-CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From Godsgalnow <@t> aol.com Sat Feb 10 10:49:01 2007 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Sat Feb 10 10:49:27 2007 Subject: [Histonet] Latest Pay Range Survey Message-ID: I use a web site called salary.com and you can put in your zip code and pull up the ranges for your area for all the job codes..........I find that the salary surveys are not always helpful. Roxanne From esther.peters <@t> verizon.net Sat Feb 10 13:43:28 2007 From: esther.peters <@t> verizon.net (Esther Peters) Date: Sat Feb 10 13:43:28 2007 Subject: [Histonet] Animal Tissue References: Message-ID: <45CE2060.70203@verizon.net> Pam, What animal tissue are you using? I recall learning from HistoNet a while back that rodent tissues should be processed for shorter periods (30 min. per solution and paraffin change compared to 60 min.) because of potential hardening issues. This is also noted in several mouse/rat or small animal processing schedules provided in the Animal Processing Manual published by the National Society for Histotechnology's Veterinary, Industry and Research Committee, edited by Gayle Callis and Diane Sterchi (along with many other helpful tips for non-human tissue handling!). I think it (or an updated version) is still available from NSH (Gayle, Diane?). Esther Peters, Ph.D. George Mason University jhnspam@aol.com wrote: > I need to get some input on cutting animal tissue. I have always worked in a > clinical setting and have recently moved into a research lab. I find that the > tissue is very brittle and needs to be iced for an extremely amount of time. > Can any of you animal cutting histo techs please give me some advice on what > type of paraffin you are using and what processing schedule you are using. I > have recently changed to Richard Allen type 9 paraffin and it seems to have > helped some. I would appreciate any advice you can give me. > > Thanks, > Pam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From amosbrooks <@t> gmail.com Sat Feb 10 16:22:48 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Feb 10 16:22:56 2007 Subject: [Histonet] Animal Tissue Message-ID: <582736990702101422u355b9e06rd0c40e0c575e8e97@mail.gmail.com> Hi, If anyone has any replies to this please do it to the list (or include me as well) as I seem to be in a similar boat having recently accepted a transfer (within the same company) to a research lab. I must admit to some trepidation about animal tissue not having much experience with it. My experiences are primarily clinical. I've heard some stories of great frustration about animal tx. I'm sure I can handle it but there is always a learning curve. Thanks Amos Brooks Message: 19 Date: Fri, 9 Feb 2007 22:57:36 EST From: jhnspam@aol.com Subject: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I need to get some input on cutting animal tissue. I have always worked in a clinical setting and have recently moved into a research lab. I find that the tissue is very brittle and needs to be iced for an extremely amount of time. Can any of you animal cutting histo techs please give me some advice on what type of paraffin you are using and what processing schedule you are using. I have recently changed to Richard Allen type 9 paraffin and it seems to have helped some. I would appreciate any advice you can give me. Thanks, Pam From JMacDonald <@t> mtsac.edu Sat Feb 10 19:49:46 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Feb 10 19:49:55 2007 Subject: [Histonet] Iron Control Message-ID: Spleen makes for a sensitive iron control. The amount of ir sufficient, but not overwhelming. Jennifer -----histonet-bounces@lists.utsou To: histonet@lists.utsouthwestern.edu From: "sheila Sent by: histonet-bounces@lists. Date: 02/10/2007 07:18AM Subject: [Histonet] Iron Hello All, We are almost out of Iron Control. We h chronic hemosiderosis condition. The Docs sa before we find a suitable control. Could any that might be easier to find? Th Sheila Adey HT MLT Port Huron Hospital Mic ____________________ ______________________ 5F__ ____________________ Your Space. Windows Live Spaces. _____ ______________________ 5F__ Histonet mailing lis Histonet@lists.utsouthwestern.edu [2]http://lists.utsouthw References 1. 3D"http://discoverspaces.live.com/?loc=en-CA" 2. 3D"http://lists.utsouthwe=/ From raj <@t> bluemarble.net Sat Feb 10 20:58:51 2007 From: raj <@t> bluemarble.net (raj) Date: Sat Feb 10 20:47:08 2007 Subject: [Histonet] Job opening Message-ID: <45CE866B.3B47CBA9@bluemarble.net> We have a opening at Bloomington Hospital for a reg. HT. Bloomington, IN. It is a day shift job. Bloomington is about 50 miles south of Indianapolis,IN. It the home of Indiana University. If interested please reply and I will direct you to the HR dept. Thank You Rebecca A. Johnson From ana.merino-trigo <@t> wanadoo.fr Sun Feb 11 10:09:40 2007 From: ana.merino-trigo <@t> wanadoo.fr (Ana MERINO-TRIGO) Date: Sun Feb 11 10:09:50 2007 Subject: [Histonet] GPR58 antibody Message-ID: <24357752.403521171210180349.JavaMail.www@wwinf1603> Hello Histonet, I was wondering if anyone has experience with GPR58, G protein coupled receptor (alias TAAR2, trace amine associated receptor 2) human antibody from LifeSpan using Ventana. So far, I've had no luck on paraffin sections. I will need to test the expression on human pancreas (paraffin sections). I'm using human cerebelum as positive control, as it's described in the literature to be expressed in this tissue. I've tested, CC1, CC2 and protease using DAB kit for the developing. With CC2 I do lose mostly of my tissues, no sure if is a buffer batch problem or if conditions are really strong for cerebellum tissue. Any advice it will be really appreciate it. thanks a lot, Ana From pamvlies <@t> sbcglobal.net Sun Feb 11 18:46:02 2007 From: pamvlies <@t> sbcglobal.net (Pam V) Date: Sun Feb 11 18:46:11 2007 Subject: [Histonet] Softening animal tissue Message-ID: <42638.38994.qm@web81115.mail.mud.yahoo.com> Hi all... One of the things I've done for years is soak tissue with a dilute glycerin. Not exactly sure why, but the cells do not blow up, nor is the staining affected, neither routine nor immunos..Sections adhere to the slides just fine. I'm at Evanston Northwestern Hospital in Evanston Illinois...we do some work on animal tissue also and this has worked well for me. It also works on decals, and very well on blood, such as bone marrow clots, as well as lymph nodes. It also allows thinner sectioning. I haven't done any recent searches for the use of glycerin and some people are hesitant, but I did see that it's suggested by Frieda Carson and is a question in the ASCP Board of Registry practice exam text. Pam Vlies HTASCP ENH Histology lab.. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 11, 2007 11:59:26 AM Subject: Histonet Digest, Vol 39, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Animal Tissue (Esther Peters) 2. Re: Animal Tissue (Amos Brooks) 3. Re: Iron Control (Jennifer MacDonald) 4. Job opening (raj) 5. GPR58 antibody (Ana MERINO-TRIGO) ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Feb 2007 14:43:28 -0500 From: Esther Peters Subject: Re: [Histonet] Animal Tissue To: jhnspam@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <45CE2060.70203@verizon.net> Content-Type: text/plain; charset=us-ascii; format=flowed Pam, What animal tissue are you using? I recall learning from HistoNet a while back that rodent tissues should be processed for shorter periods (30 min. per solution and paraffin change compared to 60 min.) because of potential hardening issues. This is also noted in several mouse/rat or small animal processing schedules provided in the Animal Processing Manual published by the National Society for Histotechnology's Veterinary, Industry and Research Committee, edited by Gayle Callis and Diane Sterchi (along with many other helpful tips for non-human tissue handling!). I think it (or an updated version) is still available from NSH (Gayle, Diane?). Esther Peters, Ph.D. George Mason University jhnspam@aol.com wrote: > I need to get some input on cutting animal tissue. I have always worked in a > clinical setting and have recently moved into a research lab. I find that the > tissue is very brittle and needs to be iced for an extremely amount of time. > Can any of you animal cutting histo techs please give me some advice on what > type of paraffin you are using and what processing schedule you are using. I > have recently changed to Richard Allen type 9 paraffin and it seems to have > helped some. I would appreciate any advice you can give me. > > Thanks, > Pam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Sat, 10 Feb 2007 17:22:48 -0500 From: "Amos Brooks" Subject: Re: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: <582736990702101422u355b9e06rd0c40e0c575e8e97@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi, If anyone has any replies to this please do it to the list (or include me as well) as I seem to be in a similar boat having recently accepted a transfer (within the same company) to a research lab. I must admit to some trepidation about animal tissue not having much experience with it. My experiences are primarily clinical. I've heard some stories of great frustration about animal tx. I'm sure I can handle it but there is always a learning curve. Thanks Amos Brooks Message: 19 Date: Fri, 9 Feb 2007 22:57:36 EST From: jhnspam@aol.com Subject: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I need to get some input on cutting animal tissue. I have always worked in a clinical setting and have recently moved into a research lab. I find that the tissue is very brittle and needs to be iced for an extremely amount of time. Can any of you animal cutting histo techs please give me some advice on what type of paraffin you are using and what processing schedule you are using. I have recently changed to Richard Allen type 9 paraffin and it seems to have helped some. I would appreciate any advice you can give me. Thanks, Pam ------------------------------ Message: 3 Date: Sat, 10 Feb 2007 17:49:46 -0800 From: Jennifer MacDonald Subject: Re: [Histonet] Iron Control To: "sheila adey" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="UTF-8" Spleen makes for a sensitive iron control. The amount of ir sufficient, but not overwhelming. Jennifer -----histonet-bounces@lists.utsou To: histonet@lists.utsouthwestern.edu From: "sheila Sent by: histonet-bounces@lists. Date: 02/10/2007 07:18AM Subject: [Histonet] Iron Hello All, We are almost out of Iron Control. We h chronic hemosiderosis condition. The Docs sa before we find a suitable control. Could any that might be easier to find? Th Sheila Adey HT MLT Port Huron Hospital Mic ____________________ ______________________ 5F__ ____________________ Your Space. Windows Live Spaces. _____ ______________________ 5F__ Histonet mailing lis Histonet@lists.utsouthwestern.edu [2]http://lists.utsouthw References 1. 3D"http://discoverspaces.live.com/?loc=en-CA"; 2. 3D"http://lists.utsouthwe=/ ------------------------------ Message: 4 Date: Sat, 10 Feb 2007 21:58:51 -0500 From: raj Subject: [Histonet] Job opening To: histonet Message-ID: <45CE866B.3B47CBA9@bluemarble.net> Content-Type: text/plain; charset=us-ascii We have a opening at Bloomington Hospital for a reg. HT. Bloomington, IN. It is a day shift job. Bloomington is about 50 miles south of Indianapolis,IN. It the home of Indiana University. If interested please reply and I will direct you to the HR dept. Thank You Rebecca A. Johnson ------------------------------ Message: 5 Date: Sun, 11 Feb 2007 17:09:40 +0100 (CET) From: Ana MERINO-TRIGO Subject: [Histonet] GPR58 antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: <24357752.403521171210180349.JavaMail.www@wwinf1603> Content-Type: text/plain; charset=UTF-8 Hello Histonet, I was wondering if anyone has experience with GPR58, G protein coupled receptor (alias TAAR2, trace amine associated receptor 2) human antibody from LifeSpan using Ventana. So far, I've had no luck on paraffin sections. I will need to test the expression on human pancreas (paraffin sections). I'm using human cerebelum as positive control, as it's described in the literature to be expressed in this tissue. I've tested, CC1, CC2 and protease using DAB kit for the developing. With CC2 I do lose mostly of my tissues, no sure if is a buffer batch problem or if conditions are really strong for cerebellum tissue. Any advice it will be really appreciate it. thanks a lot, Ana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 18 **************************************** From Diane.Gladney <@t> se.amedd.army.mil Mon Feb 12 05:57:20 2007 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Mon Feb 12 06:01:28 2007 Subject: [Histonet] Softening animal tissue In-Reply-To: <42638.38994.qm@web81115.mail.mud.yahoo.com> References: <42638.38994.qm@web81115.mail.mud.yahoo.com> Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302013F0CB8@amedmlsermc133> Pam, What is the dilution of glycerin (strength) that you use? I would be interested in trying this out. Any other guidance such as how long do you soak your blocks, etc would be helpful also. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology P.O. Box 484 4500 Stuart St. Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam V Sent: Sunday, February 11, 2007 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Softening animal tissue Hi all... One of the things I've done for years is soak tissue with a dilute glycerin. Not exactly sure why, but the cells do not blow up, nor is the staining affected, neither routine nor immunos..Sections adhere to the slides just fine. I'm at Evanston Northwestern Hospital in Evanston Illinois...we do some work on animal tissue also and this has worked well for me. It also works on decals, and very well on blood, such as bone marrow clots, as well as lymph nodes. It also allows thinner sectioning. I haven't done any recent searches for the use of glycerin and some people are hesitant, but I did see that it's suggested by Frieda Carson and is a question in the ASCP Board of Registry practice exam text. Pam Vlies HTASCP ENH Histology lab.. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 11, 2007 11:59:26 AM Subject: Histonet Digest, Vol 39, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Animal Tissue (Esther Peters) 2. Re: Animal Tissue (Amos Brooks) 3. Re: Iron Control (Jennifer MacDonald) 4. Job opening (raj) 5. GPR58 antibody (Ana MERINO-TRIGO) ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Feb 2007 14:43:28 -0500 From: Esther Peters Subject: Re: [Histonet] Animal Tissue To: jhnspam@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <45CE2060.70203@verizon.net> Content-Type: text/plain; charset=us-ascii; format=flowed Pam, What animal tissue are you using? I recall learning from HistoNet a while back that rodent tissues should be processed for shorter periods (30 min. per solution and paraffin change compared to 60 min.) because of potential hardening issues. This is also noted in several mouse/rat or small animal processing schedules provided in the Animal Processing Manual published by the National Society for Histotechnology's Veterinary, Industry and Research Committee, edited by Gayle Callis and Diane Sterchi (along with many other helpful tips for non-human tissue handling!). I think it (or an updated version) is still available from NSH (Gayle, Diane?). Esther Peters, Ph.D. George Mason University jhnspam@aol.com wrote: > I need to get some input on cutting animal tissue. I have always worked in a > clinical setting and have recently moved into a research lab. I find that the > tissue is very brittle and needs to be iced for an extremely amount of time. > Can any of you animal cutting histo techs please give me some advice on what > type of paraffin you are using and what processing schedule you are using. I > have recently changed to Richard Allen type 9 paraffin and it seems to have > helped some. I would appreciate any advice you can give me. > > Thanks, > Pam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Sat, 10 Feb 2007 17:22:48 -0500 From: "Amos Brooks" Subject: Re: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: <582736990702101422u355b9e06rd0c40e0c575e8e97@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi, If anyone has any replies to this please do it to the list (or include me as well) as I seem to be in a similar boat having recently accepted a transfer (within the same company) to a research lab. I must admit to some trepidation about animal tissue not having much experience with it. My experiences are primarily clinical. I've heard some stories of great frustration about animal tx. I'm sure I can handle it but there is always a learning curve. Thanks Amos Brooks Message: 19 Date: Fri, 9 Feb 2007 22:57:36 EST From: jhnspam@aol.com Subject: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I need to get some input on cutting animal tissue. I have always worked in a clinical setting and have recently moved into a research lab. I find that the tissue is very brittle and needs to be iced for an extremely amount of time. Can any of you animal cutting histo techs please give me some advice on what type of paraffin you are using and what processing schedule you are using. I have recently changed to Richard Allen type 9 paraffin and it seems to have helped some. I would appreciate any advice you can give me. Thanks, Pam ------------------------------ Message: 3 Date: Sat, 10 Feb 2007 17:49:46 -0800 From: Jennifer MacDonald Subject: Re: [Histonet] Iron Control To: "sheila adey" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="UTF-8" Spleen makes for a sensitive iron control. The amount of ir sufficient, but not overwhelming. Jennifer -----histonet-bounces@lists.utsou To: histonet@lists.utsouthwestern.edu From: "sheila Sent by: histonet-bounces@lists. Date: 02/10/2007 07:18AM Subject: [Histonet] Iron Hello All, We are almost out of Iron Control. We h chronic hemosiderosis condition. The Docs sa before we find a suitable control. Could any that might be easier to find? Th Sheila Adey HT MLT Port Huron Hospital Mic ____________________ ______________________ 5F__ ____________________ Your Space. Windows Live Spaces. _____ ______________________ 5F__ Histonet mailing lis Histonet@lists.utsouthwestern.edu [2]http://lists.utsouthw References 1. 3D"http://discoverspaces.live.com/?loc=en-CA"; 2. 3D"http://lists.utsouthwe=/ ------------------------------ Message: 4 Date: Sat, 10 Feb 2007 21:58:51 -0500 From: raj Subject: [Histonet] Job opening To: histonet Message-ID: <45CE866B.3B47CBA9@bluemarble.net> Content-Type: text/plain; charset=us-ascii We have a opening at Bloomington Hospital for a reg. HT. Bloomington, IN. It is a day shift job. Bloomington is about 50 miles south of Indianapolis,IN. It the home of Indiana University. If interested please reply and I will direct you to the HR dept. Thank You Rebecca A. Johnson ------------------------------ Message: 5 Date: Sun, 11 Feb 2007 17:09:40 +0100 (CET) From: Ana MERINO-TRIGO Subject: [Histonet] GPR58 antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: <24357752.403521171210180349.JavaMail.www@wwinf1603> Content-Type: text/plain; charset=UTF-8 Hello Histonet, I was wondering if anyone has experience with GPR58, G protein coupled receptor (alias TAAR2, trace amine associated receptor 2) human antibody from LifeSpan using Ventana. So far, I've had no luck on paraffin sections. I will need to test the expression on human pancreas (paraffin sections). I'm using human cerebelum as positive control, as it's described in the literature to be expressed in this tissue. I've tested, CC1, CC2 and protease using DAB kit for the developing. With CC2 I do lose mostly of my tissues, no sure if is a buffer batch problem or if conditions are really strong for cerebellum tissue. Any advice it will be really appreciate it. thanks a lot, Ana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 18 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 12 08:11:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 12 08:11:42 2007 Subject: [Histonet] Softening animal tissue In-Reply-To: <4D55B2E997EFAE4DA6081DDE100B8302013F0CB8@amedmlsermc133> Message-ID: <613076.11854.qm@web61222.mail.yahoo.com> Glycerin or glycerol is used at a 5% concentration in 70% ethanol to store blocks for long periods of time. It has proven to be advantageous to get rid of the brittleness produced in the processed tissue during dehydration. Why an alcohol (as glycerine is) would reduce the dehydration brittlenes? It is believed that is due to a change in the surface tension, to its "softness" to the touch. It is also the result of the "feeling" of change in resistance whyle sectioning a very dry tissue. It is part of the "witchcraft" side of our art, when we have been doing things for many years without knowing exactly why, without bothering much to find the explanation, and we keep doing them because it serves to the final purpose of our art, the production of a perfect section and a beautiful slide. On the other hand glycerine is used as a clearing agent, a dehydratant and a component of many staining solutoin, and those who have worked with hardened frog embryos have learned to appreciate the softening effect of glycerine. Why? Does it matter why? Ren? J. "Gladney, Diane C Ms MACH" wrote: Pam, What is the dilution of glycerin (strength) that you use? I would be interested in trying this out. Any other guidance such as how long do you soak your blocks, etc would be helpful also. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology P.O. Box 484 4500 Stuart St. Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam V Sent: Sunday, February 11, 2007 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Softening animal tissue Hi all... One of the things I've done for years is soak tissue with a dilute glycerin. Not exactly sure why, but the cells do not blow up, nor is the staining affected, neither routine nor immunos..Sections adhere to the slides just fine. I'm at Evanston Northwestern Hospital in Evanston Illinois...we do some work on animal tissue also and this has worked well for me. It also works on decals, and very well on blood, such as bone marrow clots, as well as lymph nodes. It also allows thinner sectioning. I haven't done any recent searches for the use of glycerin and some people are hesitant, but I did see that it's suggested by Frieda Carson and is a question in the ASCP Board of Registry practice exam text. Pam Vlies HTASCP ENH Histology lab.. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 11, 2007 11:59:26 AM Subject: Histonet Digest, Vol 39, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Animal Tissue (Esther Peters) 2. Re: Animal Tissue (Amos Brooks) 3. Re: Iron Control (Jennifer MacDonald) 4. Job opening (raj) 5. GPR58 antibody (Ana MERINO-TRIGO) ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Feb 2007 14:43:28 -0500 From: Esther Peters Subject: Re: [Histonet] Animal Tissue To: jhnspam@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <45CE2060.70203@verizon.net> Content-Type: text/plain; charset=us-ascii; format=flowed Pam, What animal tissue are you using? I recall learning from HistoNet a while back that rodent tissues should be processed for shorter periods (30 min. per solution and paraffin change compared to 60 min.) because of potential hardening issues. This is also noted in several mouse/rat or small animal processing schedules provided in the Animal Processing Manual published by the National Society for Histotechnology's Veterinary, Industry and Research Committee, edited by Gayle Callis and Diane Sterchi (along with many other helpful tips for non-human tissue handling!). I think it (or an updated version) is still available from NSH (Gayle, Diane?). Esther Peters, Ph.D. George Mason University jhnspam@aol.com wrote: > I need to get some input on cutting animal tissue. I have always worked in a > clinical setting and have recently moved into a research lab. I find that the > tissue is very brittle and needs to be iced for an extremely amount of time. > Can any of you animal cutting histo techs please give me some advice on what > type of paraffin you are using and what processing schedule you are using. I > have recently changed to Richard Allen type 9 paraffin and it seems to have > helped some. I would appreciate any advice you can give me. > > Thanks, > Pam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Sat, 10 Feb 2007 17:22:48 -0500 From: "Amos Brooks" Subject: Re: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: <582736990702101422u355b9e06rd0c40e0c575e8e97@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi, If anyone has any replies to this please do it to the list (or include me as well) as I seem to be in a similar boat having recently accepted a transfer (within the same company) to a research lab. I must admit to some trepidation about animal tissue not having much experience with it. My experiences are primarily clinical. I've heard some stories of great frustration about animal tx. I'm sure I can handle it but there is always a learning curve. Thanks Amos Brooks Message: 19 Date: Fri, 9 Feb 2007 22:57:36 EST From: jhnspam@aol.com Subject: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I need to get some input on cutting animal tissue. I have always worked in a clinical setting and have recently moved into a research lab. I find that the tissue is very brittle and needs to be iced for an extremely amount of time. Can any of you animal cutting histo techs please give me some advice on what type of paraffin you are using and what processing schedule you are using. I have recently changed to Richard Allen type 9 paraffin and it seems to have helped some. I would appreciate any advice you can give me. Thanks, Pam ------------------------------ Message: 3 Date: Sat, 10 Feb 2007 17:49:46 -0800 From: Jennifer MacDonald Subject: Re: [Histonet] Iron Control To: "sheila adey" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="UTF-8" Spleen makes for a sensitive iron control. The amount of ir sufficient, but not overwhelming. Jennifer -----histonet-bounces@lists.utsou To: histonet@lists.utsouthwestern.edu From: "sheila Sent by: histonet-bounces@lists. Date: 02/10/2007 07:18AM Subject: [Histonet] Iron Hello All, We are almost out of Iron Control. We h chronic hemosiderosis condition. The Docs sa before we find a suitable control. Could any that might be easier to find? Th Sheila Adey HT MLT Port Huron Hospital Mic ____________________ ______________________ 5F__ ____________________ Your Space. Windows Live Spaces. _____ ______________________ 5F__ Histonet mailing lis Histonet@lists.utsouthwestern.edu [2]http://lists.utsouthw References 1. 3D"http://discoverspaces.live.com/?loc=en-CA"; 2. 3D"http://lists.utsouthwe=/ ------------------------------ Message: 4 Date: Sat, 10 Feb 2007 21:58:51 -0500 From: raj Subject: [Histonet] Job opening To: histonet Message-ID: <45CE866B.3B47CBA9@bluemarble.net> Content-Type: text/plain; charset=us-ascii We have a opening at Bloomington Hospital for a reg. HT. Bloomington, IN. It is a day shift job. Bloomington is about 50 miles south of Indianapolis,IN. It the home of Indiana University. If interested please reply and I will direct you to the HR dept. Thank You Rebecca A. Johnson ------------------------------ Message: 5 Date: Sun, 11 Feb 2007 17:09:40 +0100 (CET) From: Ana MERINO-TRIGO Subject: [Histonet] GPR58 antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: <24357752.403521171210180349.JavaMail.www@wwinf1603> Content-Type: text/plain; charset=UTF-8 Hello Histonet, I was wondering if anyone has experience with GPR58, G protein coupled receptor (alias TAAR2, trace amine associated receptor 2) human antibody from LifeSpan using Ventana. So far, I've had no luck on paraffin sections. I will need to test the expression on human pancreas (paraffin sections). I'm using human cerebelum as positive control, as it's described in the literature to be expressed in this tissue. I've tested, CC1, CC2 and protease using DAB kit for the developing. With CC2 I do lose mostly of my tissues, no sure if is a buffer batch problem or if conditions are really strong for cerebellum tissue. Any advice it will be really appreciate it. thanks a lot, Ana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 18 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From Michelle.Perrins <@t> uct.ac.za Mon Feb 12 08:27:00 2007 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Mon Feb 12 08:27:25 2007 Subject: [Histonet] Hi Message-ID: <45D09553.A704.0070.0@uct.ac.za> Hi I am trying out a xylene substitute, namely Ottix Plus as well as Ottix Shaper(alcohol substitute) in my rotary tissue processor and in my automatic stainer. I would like to know if anybody has heard of/tried this product or any other xylene substitute and what your comments are. Many thanks Regards Michelle Perrins Forensic Pathology Services Department of Health Western Cape South Africa 021 447 1496 fax: 021 448 1249 From rjbuesa <@t> yahoo.com Mon Feb 12 08:34:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 12 08:34:40 2007 Subject: [Histonet] Hi In-Reply-To: <45D09553.A704.0070.0@uct.ac.za> Message-ID: <128737.49540.qm@web61223.mail.yahoo.com> Michelle: First of all try to get the MSDS of the product you are going to start using because some of them are not as innocuous as they alledge to be; some may have TWA and STEL values as high as the product they are intended to substitute. Beware! Ren? J. Michelle Perrins wrote: Hi I am trying out a xylene substitute, namely Ottix Plus as well as Ottix Shaper(alcohol substitute) in my rotary tissue processor and in my automatic stainer. I would like to know if anybody has heard of/tried this product or any other xylene substitute and what your comments are. Many thanks Regards Michelle Perrins Forensic Pathology Services Department of Health Western Cape South Africa 021 447 1496 fax: 021 448 1249 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never Miss an Email Stay connected with Yahoo! Mail on your mobile. Get started! From Chris.Goodall <@t> bristol.ac.uk Mon Feb 12 08:37:37 2007 From: Chris.Goodall <@t> bristol.ac.uk (CL Goodall, Anatomy) Date: Mon Feb 12 08:38:08 2007 Subject: [Histonet] PMV Cryomicrotome Message-ID: Hi histonetters, does anyone know of a service engineer for the PMV450 whole body cryomicrotome? PMV were taken over by LKB, who were subsequently taken over by Leica and they no longer service PMV equipment. Any info would be gratefully received. ---------------------- CL Goodall, Anatomy Chris.Goodall@bristol.ac.uk From wiscarrow <@t> serha.ca Mon Feb 12 08:38:41 2007 From: wiscarrow <@t> serha.ca (Scarrow, William (R1SE)) Date: Mon Feb 12 08:38:54 2007 Subject: [Histonet] (no subject) Message-ID: <2BCC0130EEF44B47BDE1B9715C1D56B0810811@RHAEX1.RHA-RRS.CA> Was not aware of the large number of continuous e-mails that I would receive....please delete me from the mailing list thank you WG SCARROW SUPERVISOR HISTOPATHOLOGY SERHA ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain priviledged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From joost.bruijntjes <@t> tno.nl Mon Feb 12 09:23:00 2007 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Mon Feb 12 09:23:17 2007 Subject: [Histonet] (no subject) Message-ID: <1A7F09509ACB294B835A94B2C8EB50F402BBAD4C@MS-DT01VS01.tsn.tno.nl> Hi All Is anyone of you aware of a good and general article about in situ hybridization? Thanks in advance Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From gcallis <@t> montana.edu Mon Feb 12 09:59:25 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Feb 12 09:59:40 2007 Subject: sectioning Re: [Histonet] Animal Tissue In-Reply-To: References: Message-ID: <6.0.0.22.1.20070212085617.01b26030@gemini.msu.montana.edu> Animal tissue is very lean, and you probably are over dehydrating or drying out the tissue during processing. Our mouse tissues are on a much shorter processing schedule than we ever used for human tissues (which tend to be a bit more fatty). Do not add heat to processing schedule (solvents), and cut back on times in all solvents. The smaller the tissue i.e. lymph nodes and slices of brain is on a much shorter schedule here. You paraffin temperatures should be at 60C or less. At 08:57 PM 2/9/2007, you wrote: >I need to get some input on cutting animal tissue. I have always worked in a >clinical setting and have recently moved into a research lab. I find that the > tissue is very brittle and needs to be iced for an extremely amount of > time. >Can any of you animal cutting histo techs please give me some advice on what >type of paraffin you are using and what processing schedule you are using. I >have recently changed to Richard Allen type 9 paraffin and it seems to have >helped some. I would appreciate any advice you can give me. > >Thanks, >Pam >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From TillRenee <@t> uams.edu Mon Feb 12 10:08:51 2007 From: TillRenee <@t> uams.edu (Till, Renee) Date: Mon Feb 12 10:09:08 2007 Subject: [Histonet] Prolong gold tips Message-ID: <11F927674DEBDC43B960809A7403C5D2035F8B81@MAILPED.ad.uams.edu> Quite a few people seem to use this so I thought I'd try it and see if there is any difference from Vectashield. I have tried the versions of both that have DAPI. Couple of questions about the prolong. How long do you usually let it cure before looking at the slides? How long before you seal the slides? Also, it says to remove the excess moisture. How picky is it on this? The first few slides I tried were not optimally done, but still it did not seem as good as some of my slides with Vectashield. The DAPI was less bright and the intensity varied in areas. I wondered if this was because I might have got parts of the tissue drier than others (I blotted with a kimwipe). Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 From tp2 <@t> medicine.wisc.edu Mon Feb 12 10:17:39 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Mon Feb 12 10:18:17 2007 Subject: [Histonet] Prolong gold tips Message-ID: <45D03EC3020000DF00004E85@gwmail.medicine.wisc.edu> I've used Prolong Gold before. Typically, I put it on the slide, coverslip, turn the slide upside down on a wipe all, press down on the slide to remove excess, then put the slide in a 37 degree oven for about a half an hour. You might also try SlowFade Gold w/ DAPI. Tom Pier >>> "Till, Renee" 02/12/07 10:08 AM >>> Quite a few people seem to use this so I thought I'd try it and see if there is any difference from Vectashield. I have tried the versions of both that have DAPI. Couple of questions about the prolong. How long do you usually let it cure before looking at the slides? How long before you seal the slides? Also, it says to remove the excess moisture. How picky is it on this? The first few slides I tried were not optimally done, but still it did not seem as good as some of my slides with Vectashield. The DAPI was less bright and the intensity varied in areas. I wondered if this was because I might have got parts of the tissue drier than others (I blotted with a kimwipe). Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wiscarrow <@t> serha.ca Mon Feb 12 10:28:56 2007 From: wiscarrow <@t> serha.ca (Scarrow, William (R1SE)) Date: Mon Feb 12 10:31:06 2007 Subject: [Histonet] unsubscribe Message-ID: <2BCC0130EEF44B47BDE1B9715C1D56B0810815@RHAEX1.RHA-RRS.CA> WG SCARROW SUPERVISOR HISTOPATHOLOGY SERHA ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain priviledged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From jwatson <@t> gnf.org Mon Feb 12 10:35:47 2007 From: jwatson <@t> gnf.org (James Watson) Date: Mon Feb 12 10:36:02 2007 Subject: [Histonet] Softening animal tissue Message-ID: Diane, For years I have been using 5% glycerin in my absolute alcohol on the tissue processor for animal tissue here is a the program for mouse tissue. Mouse Tissue Processing Schedule Station Reagent Temperature Vacuum Time Drain Time Agitate 1 70% ETOH Ambient OFF 0:30 30 Off 2 80% ETOH Ambient Vacuum 0:30 30 Stirred 3 95% ETOH Ambient Vacuum 0:30 30 Stirred 4 95% ETOH Ambient Vacuum 0:45 45 T&S 5 5% Glycerin in100% ETOH Ambient Vacuum 0:30 30 Stirred 6 5% Glycerin in 100% ETOH Ambient Vacuum 0:30 30 Stirred 7 5% Glycerin in 100% ETOH Ambient V&P 0:45 100 T&S 8 Xylene Ambient Vacuum 0:30 45 Stirred 9 Xylene Ambient Vacuum 0:30 45 Stirred 10 Xylene Ambient V&P 0:45 120 T&S 11 Paraffin 60 V&P 0:30 120 Stirred 12 Paraffin 60 V&P 0:30 120 Stirred 13 Paraffin 60 V&P 0:45 120 Stirred 14 Paraffin 60 V&P 0:45 120 Stirred You will still need to soak the tissue before cutting, but it gives good infiltration and soaking times are decreased. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gladney, Diane C Ms MACH Sent: Monday, February 12, 2007 3:57 AM To: Pam V; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Softening animal tissue Pam, What is the dilution of glycerin (strength) that you use? I would be interested in trying this out. Any other guidance such as how long do you soak your blocks, etc would be helpful also. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology P.O. Box 484 4500 Stuart St. Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam V Sent: Sunday, February 11, 2007 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Softening animal tissue Hi all... One of the things I've done for years is soak tissue with a dilute glycerin. Not exactly sure why, but the cells do not blow up, nor is the staining affected, neither routine nor immunos..Sections adhere to the slides just fine. I'm at Evanston Northwestern Hospital in Evanston Illinois...we do some work on animal tissue also and this has worked well for me. It also works on decals, and very well on blood, such as bone marrow clots, as well as lymph nodes. It also allows thinner sectioning. I haven't done any recent searches for the use of glycerin and some people are hesitant, but I did see that it's suggested by Frieda Carson and is a question in the ASCP Board of Registry practice exam text. Pam Vlies HTASCP ENH Histology lab.. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 11, 2007 11:59:26 AM Subject: Histonet Digest, Vol 39, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Animal Tissue (Esther Peters) 2. Re: Animal Tissue (Amos Brooks) 3. Re: Iron Control (Jennifer MacDonald) 4. Job opening (raj) 5. GPR58 antibody (Ana MERINO-TRIGO) ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Feb 2007 14:43:28 -0500 From: Esther Peters Subject: Re: [Histonet] Animal Tissue To: jhnspam@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <45CE2060.70203@verizon.net> Content-Type: text/plain; charset=us-ascii; format=flowed Pam, What animal tissue are you using? I recall learning from HistoNet a while back that rodent tissues should be processed for shorter periods (30 min. per solution and paraffin change compared to 60 min.) because of potential hardening issues. This is also noted in several mouse/rat or small animal processing schedules provided in the Animal Processing Manual published by the National Society for Histotechnology's Veterinary, Industry and Research Committee, edited by Gayle Callis and Diane Sterchi (along with many other helpful tips for non-human tissue handling!). I think it (or an updated version) is still available from NSH (Gayle, Diane?). Esther Peters, Ph.D. George Mason University jhnspam@aol.com wrote: > I need to get some input on cutting animal tissue. I have always worked in a > clinical setting and have recently moved into a research lab. I find that the > tissue is very brittle and needs to be iced for an extremely amount of time. > Can any of you animal cutting histo techs please give me some advice on what > type of paraffin you are using and what processing schedule you are using. I > have recently changed to Richard Allen type 9 paraffin and it seems to have > helped some. I would appreciate any advice you can give me. > > Thanks, > Pam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Sat, 10 Feb 2007 17:22:48 -0500 From: "Amos Brooks" Subject: Re: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: <582736990702101422u355b9e06rd0c40e0c575e8e97@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi, If anyone has any replies to this please do it to the list (or include me as well) as I seem to be in a similar boat having recently accepted a transfer (within the same company) to a research lab. I must admit to some trepidation about animal tissue not having much experience with it. My experiences are primarily clinical. I've heard some stories of great frustration about animal tx. I'm sure I can handle it but there is always a learning curve. Thanks Amos Brooks Message: 19 Date: Fri, 9 Feb 2007 22:57:36 EST From: jhnspam@aol.com Subject: [Histonet] Animal Tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I need to get some input on cutting animal tissue. I have always worked in a clinical setting and have recently moved into a research lab. I find that the tissue is very brittle and needs to be iced for an extremely amount of time. Can any of you animal cutting histo techs please give me some advice on what type of paraffin you are using and what processing schedule you are using. I have recently changed to Richard Allen type 9 paraffin and it seems to have helped some. I would appreciate any advice you can give me. Thanks, Pam ------------------------------ Message: 3 Date: Sat, 10 Feb 2007 17:49:46 -0800 From: Jennifer MacDonald Subject: Re: [Histonet] Iron Control To: "sheila adey" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="UTF-8" Spleen makes for a sensitive iron control. The amount of ir sufficient, but not overwhelming. Jennifer -----histonet-bounces@lists.utsou To: histonet@lists.utsouthwestern.edu From: "sheila Sent by: histonet-bounces@lists. Date: 02/10/2007 07:18AM Subject: [Histonet] Iron Hello All, We are almost out of Iron Control. We h chronic hemosiderosis condition. The Docs sa before we find a suitable control. Could any that might be easier to find? Th Sheila Adey HT MLT Port Huron Hospital Mic ____________________ ______________________ 5F__ ____________________ Your Space. Windows Live Spaces. _____ ______________________ 5F__ Histonet mailing lis Histonet@lists.utsouthwestern.edu [2]http://lists.utsouthw References 1. 3D"http://discoverspaces.live.com/?loc=en-CA"; 2. 3D"http://lists.utsouthwe=/ ------------------------------ Message: 4 Date: Sat, 10 Feb 2007 21:58:51 -0500 From: raj Subject: [Histonet] Job opening To: histonet Message-ID: <45CE866B.3B47CBA9@bluemarble.net> Content-Type: text/plain; charset=us-ascii We have a opening at Bloomington Hospital for a reg. HT. Bloomington, IN. It is a day shift job. Bloomington is about 50 miles south of Indianapolis,IN. It the home of Indiana University. If interested please reply and I will direct you to the HR dept. Thank You Rebecca A. Johnson ------------------------------ Message: 5 Date: Sun, 11 Feb 2007 17:09:40 +0100 (CET) From: Ana MERINO-TRIGO Subject: [Histonet] GPR58 antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: <24357752.403521171210180349.JavaMail.www@wwinf1603> Content-Type: text/plain; charset=UTF-8 Hello Histonet, I was wondering if anyone has experience with GPR58, G protein coupled receptor (alias TAAR2, trace amine associated receptor 2) human antibody from LifeSpan using Ventana. So far, I've had no luck on paraffin sections. I will need to test the expression on human pancreas (paraffin sections). I'm using human cerebelum as positive control, as it's described in the literature to be expressed in this tissue. I've tested, CC1, CC2 and protease using DAB kit for the developing. With CC2 I do lose mostly of my tissues, no sure if is a buffer batch problem or if conditions are really strong for cerebellum tissue. Any advice it will be really appreciate it. thanks a lot, Ana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 18 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> gmhsc.com Mon Feb 12 10:56:22 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Mon Feb 12 10:56:36 2007 Subject: [Histonet] deio. or tap water on H&E's Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From pruegg <@t> ihctech.net Mon Feb 12 10:56:47 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Feb 12 10:57:03 2007 Subject: [Histonet] (no subject) In-Reply-To: <1A7F09509ACB294B835A94B2C8EB50F402BBAD4C@MS-DT01VS01.tsn.tno.nl> Message-ID: <005d01c74ec6$c90d9470$6501a8c0@Patsy> Here is a really good handbook on ISH. In Situ Hybridization. Royal Microscopical Society Microscopy Handbooks. Leitch, A.R et al. Bios Scientific Publishers Ltd. 1994. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Monday, February 12, 2007 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi All Is anyone of you aware of a good and general article about in situ hybridization? Thanks in advance Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Mon Feb 12 11:01:02 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Feb 12 11:02:07 2007 Subject: [Histonet] deio. or tap water on H&E's In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30F49@PHSXMB1.partners.org> Tap water for washing. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy Self Sent: Monday, February 12, 2007 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] deio. or tap water on H&E's Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION. From wiscarrow <@t> serha.ca Mon Feb 12 11:06:50 2007 From: wiscarrow <@t> serha.ca (Scarrow, William (R1SE)) Date: Mon Feb 12 11:07:01 2007 Subject: [Histonet] unsubscribe Message-ID: <2BCC0130EEF44B47BDE1B9715C1D56B0810816@RHAEX1.RHA-RRS.CA> ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain priviledged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From TJJ <@t> Stowers-Institute.org Mon Feb 12 11:06:48 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Feb 12 11:07:11 2007 Subject: [Histonet] Re: Animal tissue Message-ID: Consider changing your clearants from xylene to the xylene substitutes, it'll help make your tissues less brittle. We have one station of xylene (due to a relatively high summer humidity - and its increased tolerance for water carryover) and then two stations of aliphatic hydrocarbon (the stuff that does not smell like oranges). Although the Limonenes would work very well, many people have a hypersensitivity to the odor. I ditto what everybody else said about less time in the dehydrating stations as well. Most of our mouse samples do well with 20-30 minutes per station. We do recommend you dissect larger organs, like liver, and bisect things like kidney, testis, heart and brain for better fluid penetration. We fix our samples off the processor for whatever the recommended time is, and store them in 70% alcohol until ready to process. Our first station on the processor is 70% alcohol. We have also added glycerol to 100% alcohol (5% v/v) for processing some samples like pancreas (20 minutes/station) and femur (more extended processing schedule). For sectioning samples that seem to be a bit too dry, brief soaking with ice water or dilute downy usually works well. You might also benefit by using a different paraffin, one especially formulated for infiltration. As a last bit of advice, keep your embedding paraffin stirred well before using to keep the polymers in solution for easier sectioning. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From TownsendD <@t> childrensdayton.org Mon Feb 12 11:21:48 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Mon Feb 12 11:22:17 2007 Subject: [Histonet] deio. or tap water on H&E's Message-ID: We use tap water: unless you have very chlorinated water, there is no need for deionized water. Beside, tap water helps in the bluing after hematoxylin. Dolores From Wanda.Smith <@t> HCAhealthcare.com Mon Feb 12 12:12:25 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Mon Feb 12 12:12:49 2007 Subject: [Histonet] deio. or tap water on H&E's In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> References: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> Message-ID: Amy, We have tap water hooked up to our Leica AutoStainer. Wanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Monday, February 12, 2007 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] deio. or tap water on H&E's Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duncanwood1 <@t> fsmail.net Mon Feb 12 12:29:16 2007 From: duncanwood1 <@t> fsmail.net (Duncan Wood) Date: Mon Feb 12 12:29:27 2007 Subject: [Histonet] RE: Histonet Digest, Vol 39, Issue 20 Message-ID: <15428984.461701171304956415.JavaMail.www@wwinf3101> You need either tap water (if you are in a hard water area) or use Scott's Tap Water substitute to get consistent 'bluing' results. Regards Duncan wood Consultant, Clin-Tech Ltd (UK) ======================================== Message Received: Feb 12 2007, 06:01 PM From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Cc: Subject: Histonet Digest, Vol 39, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: deio. or tap water on H&E's (Dolores Townsend) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Feb 2007 12:21:48 -0500 From: "Dolores Townsend" Subject: Re: [Histonet] deio. or tap water on H&E's To: ASelf@gmhsc.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII We use tap water: unless you have very chlorinated water, there is no need for deionized water. Beside, tap water helps in the bluing after hematoxylin. Dolores ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 20 **************************************** From GDawson <@t> dynacaremilwaukee.com Mon Feb 12 12:30:59 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Feb 12 12:31:17 2007 Subject: [Histonet] deio. or tap water on H&E's Message-ID: TAP H2O. Glen Dawson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy Self Sent: Monday, February 12, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] deio. or tap water on H&E's Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Feb 12 13:02:32 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Feb 12 13:02:51 2007 Subject: [Histonet] MIB-1 IHC on porcine tissue? Message-ID: <45D073780200007700004394@hcnwgwds01.hh.chs> Does Ki-67 (clone MIB-1 ) work on formalin-fixed, paraffin-embedded porcine tissue? If not, are there any antibodies out there that you can use to study cellular proliferation in porcine tissue? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Diane.Gladney <@t> se.amedd.army.mil Mon Feb 12 13:20:46 2007 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Mon Feb 12 13:21:28 2007 Subject: [Histonet] deio. or tap water on H&E's In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> References: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> Message-ID: <4D55B2E997EFAE4DA6081DDE100B83020142D7DD@amedmlsermc133> We use tap water which is hooked up to our Leica Auto-stainer. We have never had a problem with too much chlorine in the water. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC? 29207 Email:? diane.gladney@se.amedd.army.mil Phone:? 803-751-2530 FAX:???? 803-751-7829 DSN:??? 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Monday, February 12, 2007 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] deio. or tap water on H&E's Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shawnster73 <@t> aol.com Mon Feb 12 13:47:50 2007 From: shawnster73 <@t> aol.com (shawnster73@aol.com) Date: Mon Feb 12 13:48:05 2007 Subject: [Histonet] HELP!!! vertical IHC staining artifact and steamer retrival Message-ID: <8C91CF5CFC8E63F-14F0-554D@MBLK-M42.sysops.aol.com> I'm hoping that you will be able to help me with my staining problem. I am using a vector ABC kit and staining rat lung with ULEX stain. I am using a steamer for 50 minutes for retrival before staining. I retriving in staining dishes that hold 20 slides. I have tried just putting one dish into the steamer and tried puttng 2 dishes into the steamer at a time and still get the same results. I have also tried using the same staining dishes in the same steamer, using the same protocol, but only stained 2 slides and the staining came out beautiful. I have had suggestions of everything from the pap pen caused the problem to the retrival caused it. I am suspicious of the steamer. If you were using a rack in the steamer, is there a distace that you would keep between the slides to ensure proper solution movement so that the slides are not cooked? Any suggestions at all that might help solve my problem would be greatly appreciated. ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From vazquezr <@t> ohsu.edu Mon Feb 12 13:53:39 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Feb 12 13:54:15 2007 Subject: [Histonet] deio. or tap water on H&E's Message-ID: Amy, I use tap for rinsing and deionized in my bluing. Robyn CHH of OHSU >>> "Amy Self" 2/12/2007 8:56 AM >>> Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon Feb 12 14:12:53 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Feb 12 14:13:25 2007 Subject: [Histonet] Bar code readers Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D336A@sjhaexc02.sjha.org> Would those of you using bar code readers/scanners please share your experience with me? We are upgrading our LIS and hopefully will be able to bar code cases, specimens, blocks and slides. Any information would be greatly appreciated. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From SBaldwin <@t> compucyte.com Mon Feb 12 13:38:25 2007 From: SBaldwin <@t> compucyte.com (Scott Baldwin) Date: Mon Feb 12 14:23:31 2007 Subject: [Histonet] Cold Spring Harbor Symposium and Agenda Message-ID: This is a quick email to invite you to attend the Quantitative Imaging Cytometry Symposium hosted by Cold Spring Harbor Laboratories taking place in Woodbury, Long Island, NY on February 20. The scientific content of the symposium reflects focus on targeted drug discovery and companion biomarker validation, more specifically tissue and cell-based biomarkers. Presentations relevant to tissue analysis are highlighted below: 1. "An introduction to Laser Scanning Cytometry: Individuals Cells and Whole Tissues" (LSC technology and applications update, with focus on apoptosis and tissue analysis), William Telford, PhD, Core Flow Cytometry, NIH/NCI, Bethesda, MD 2. "Quantitative analysis of JNK signaling in a mouse model of Parkinsons disease by Laser Scanning Cytometry" - Deirdre Buckley, PhD, Cold Spring Harbor Laborator y 3. "Pharmacodynamic monitoring of molecular cancer therapeutics at the preclinical and early clinical stages of development" - David W Hedley, MD, Dept. of Medical Oncology and Hematology, Division of Experimental Therapeutics, Princess Margaret Hospital/Ontario Cancer Institute, University of Toronto 4. "An automated method to monitor skin mast cells by Laser Scanning Cytometry" Gloria Juan, Sr. Scientist, Clinical Immunology, Amgen 5. "LSC analysis of tissue microarrays: Application to subcellular localization of p27 and prostate cancer recurrence" - Peter Gann, MD, ScD, Department of Pathology, College of Medicine, University of Illinois at Chicago 6. "Quantitative Imaging Cytometry: Technology to support the new role of pathology in the era of specific targeted therapies"- William Geddie, MD, Department of Laboratory Medicine and Pathobiology, University of Toronto 7. "Quantification of Three Color Quantum Dot Labeled Pancreatic Hormones using the Laser Scanning Cytometer" - David Krull, Senior Scientist, Molecular and Ultrastructural Pathology, GlaxoSmithKline Safety Assessment, Research Triangle Park, NC Full agenda and registration: http://www.compucyte.com/Agenda%20-%20CSH%20Advances%20in%20Laser%20Cytometry-2007.htm Scott Baldwin MT(ASCP) From Charlene.Henry <@t> STJUDE.ORG Mon Feb 12 15:05:16 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Feb 12 15:05:25 2007 Subject: [Histonet] Bar code readers. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1FEE@SJMEMXMB02.stjude.sjcrh.local> I would like to hear your responses also especially if anyone is using the "Specimen Verification System" from Thermo Electron. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 12, 2007 2:13 PM To: Histonet Subject: [Histonet] Bar code readers. . Would those of you using bar code readers/scanners please share your experience with me? We are upgrading our LIS and hopefully will be able to bar code cases, specimens, blocks and slides. Any information would be greatly appreciated. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From mward <@t> wfubmc.edu Mon Feb 12 15:08:56 2007 From: mward <@t> wfubmc.edu (Martha Ward) Date: Mon Feb 12 15:09:08 2007 Subject: [Histonet] FAS (CD95) Message-ID: <61135F0455D33347B5AAE209B903A304190A855D@EXCHVS2.medctr.ad.wfubmc.edu> I have been contacted about doing a research project with this antibody. They provided me with one from Chemicon, but I am having no luck with staining. I would appreciate any information about getting this to work in ffpe samples. Thank you in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From Vickroy.Jim <@t> mhsil.com Mon Feb 12 15:10:12 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Feb 12 15:10:21 2007 Subject: [Histonet] interaction of techs with pathologist assistants Message-ID: I would appreciate information from other institutions on how much technical assistance a pathologist assistant requires or gets when doing grossing of specimens. Our pa's handle most of the larger specimens and many of my techs think that the pa's need more assistance here than at other institutions. I realize that duties and expectations probably vary but I am trying to get a handle on usual practices. Most of us have been around before pa's existed and have always assisted pathologists but are somewhat vague on how much help a pa should have. I would appreciate any information you would have. Currently we having techs grossing small surgicals by themselves and assisting pa's with the larger surgical specimens. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From doug <@t> ppspath.com Mon Feb 12 15:27:18 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Feb 12 15:25:09 2007 Subject: [Histonet] interaction of techs with pathologist assistants In-Reply-To: Message-ID: I am not sure what type of assistance you are referring to but the PA's that I have been around are pretty self sufficient. They can make their own cassettes and clean up their own bench. Can you afford taking your techs away from technical work to assist a PA? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, February 12, 2007 4:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interaction of techs with pathologist assistants I would appreciate information from other institutions on how much technical assistance a pathologist assistant requires or gets when doing grossing of specimens. Our pa's handle most of the larger specimens and many of my techs think that the pa's need more assistance here than at other institutions. I realize that duties and expectations probably vary but I am trying to get a handle on usual practices. Most of us have been around before pa's existed and have always assisted pathologists but are somewhat vague on how much help a pa should have. I would appreciate any information you would have. Currently we having techs grossing small surgicals by themselves and assisting pa's with the larger surgical specimens. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Mon Feb 12 15:28:51 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Feb 12 15:29:04 2007 Subject: [Histonet] interaction of techs with pathologist assistants Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4AF@EXCHANGEBE1.carle.com> I am not sure what you actually mean by assist. Everywhere I have worked the techs helped me the same as they would have a pathologist. Here at Carle the lab assistants accession the cases, label the blocks and set up the grossing areas. After I gross they move the blocks to the processors. Before I started here the pathologists had a tech stand there when they grossed and put the lids on the cassettes as they were filled. I didn't really want that much help and put my own lids on. Other than that the system hasn't changed here. Charles Embrey, PA(ASCP) Carle Clinic Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, February 12, 2007 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interaction of techs with pathologist assistants I would appreciate information from other institutions on how much technical assistance a pathologist assistant requires or gets when doing grossing of specimens. Our pa's handle most of the larger specimens and many of my techs think that the pa's need more assistance here than at other institutions. I realize that duties and expectations probably vary but I am trying to get a handle on usual practices. Most of us have been around before pa's existed and have always assisted pathologists but are somewhat vague on how much help a pa should have. I would appreciate any information you would have. Currently we having techs grossing small surgicals by themselves and assisting pa's with the larger surgical specimens. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mary.Vaughan <@t> RoswellPark.org Mon Feb 12 15:50:15 2007 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Mon Feb 12 15:50:27 2007 Subject: [Histonet] HPV Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE0BE0F7F6@VERITY.roswellpark.org> Hi everyone - I was hoping some of you... my clinical friends, could recommend a good HPV antibody. I'm in research and have a human project that requires me to do this Ab. Thanks. Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From macantu <@t> mdanderson.org Mon Feb 12 16:13:01 2007 From: macantu <@t> mdanderson.org (macantu@mdanderson.org) Date: Mon Feb 12 16:13:27 2007 Subject: [Histonet] PD-L1 in paraffin Message-ID: Dear Histonetters, This is my first time using this histosearch email. I just discovered it a few weeks ago. My lab is wanting to start looking for B7-H1 (PD-L1) in human tumor tissues that were fixed in paraffin. I have this working in frozen tissue ( HRP detection kit from BD) but haven't been able to get it to work in paraffin. And I cant find a commercially available antibody that says it works in paraffin. Does anyone know of a commercially available B7-H1 (PD-L1) antibody for human paraffin tissues? Thank you in advance, Mayra Research Assistant II Dept. of Melanoma Medical Oncology MD Anderson Cancer Center Houston, Texas Tel. 713-563-9177 From katherine-walters <@t> uiowa.edu Mon Feb 12 16:23:26 2007 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Feb 12 16:23:39 2007 Subject: [Histonet] circularly polarized light microscope Message-ID: Hi everyone, I am looking for a microscope to use for quantifying my Sirius Red stained samples. I have looked on line and in the Histonet archives, but I am unsure of what type of quality microscope I need to get a good quantitation. I have seen some relatively inexpensive scopes from a company called LOMO, but I don't know anything about them. Has anyone done this before? Do you know if I can get this type of information from just throwing in a couple of quarter-wave plates to our linearly polarized microscope? Any comments would be appreciated. Thanks, Kathy From soofias2 <@t> yahoo.com Mon Feb 12 16:54:59 2007 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Mon Feb 12 16:55:07 2007 Subject: [Histonet] FAS (CD95) In-Reply-To: <61135F0455D33347B5AAE209B903A304190A855D@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <516594.83092.qm@web39501.mail.mud.yahoo.com> I am using CD95 from from Dako # M3554 and from Alexis cat# 805-022C100,but I am using on human skin frozen sections. You may contact companies ands ask about your specific use. Good Luck! soofia Martha Ward wrote: I have been contacted about doing a research project with this antibody. They provided me with one from Chemicon, but I am having no luck with staining. I would appreciate any information about getting this to work in ffpe samples. Thank you in advance for your help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From jnocito <@t> satx.rr.com Mon Feb 12 17:10:33 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Feb 12 17:09:53 2007 Subject: [Histonet] deio. or tap water on H&E's References: <39836CD6DB61654E8F95A35898C9218602E4F081@exchange.gmhpost.com> Message-ID: <003e01c74efa$fff3d970$649eae18@yourxhtr8hvc4p> definitely tap. Water has this bad habit of trying to equalize itself. Running DI water through a stainer will eventually corrode the metal fittings because the water will pick up metal molecules from the metal fittings. Just my 3 cents. Joe ----- Original Message ----- From: "Amy Self" To: Sent: Monday, February 12, 2007 10:56 AM Subject: [Histonet] deio. or tap water on H&E's Dear Histonetters, How many of you use deionized water and how many use tap water in your H&E stain set-up? Thanks in advance, Amy Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Feb 12 17:13:42 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Feb 12 17:13:01 2007 Subject: [Histonet] Hi References: <128737.49540.qm@web61223.mail.yahoo.com> Message-ID: <005b01c74efb$70e4d030$649eae18@yourxhtr8hvc4p> Michelle, I have to agree with Rene on this. Some products are not what they are supposed to be and may be as hazardous as say xylene or 10% NBF. I'm not mentioning any products. Y'all understand, don't you? Joe, not ready to be nuked again ----- Original Message ----- From: "Rene J Buesa" To: "Michelle Perrins" ; Sent: Monday, February 12, 2007 8:34 AM Subject: Re: [Histonet] Hi > Michelle: > First of all try to get the MSDS of the product you are going to start > using because some of them are not as innocuous as they alledge to be; > some may have TWA and STEL values as high as the product they are intended > to substitute. Beware! > Ren? J. > > Michelle Perrins wrote: > Hi > I am trying out a xylene substitute, namely Ottix Plus as well as Ottix > Shaper(alcohol substitute) in my rotary tissue processor and in my > automatic stainer. > I would like to know if anybody has heard of/tried this product or any > other xylene substitute and what your comments are. > Many thanks > > Regards > > Michelle Perrins > Forensic Pathology Services > Department of Health > Western Cape > South Africa > > 021 447 1496 > > fax: 021 448 1249 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Never Miss an Email > Stay connected with Yahoo! Mail on your mobile. Get started! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> bidmc.harvard.edu Mon Feb 12 20:16:16 2007 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Mon Feb 12 20:14:07 2007 Subject: [Histonet] rabbit noggin antibody Message-ID: Hello, Can anyone recommend a good antibody for noggin? It will be used for western blot and staining in rabbit tissues. Thanks, Caroline From louise.renton <@t> gmail.com Tue Feb 13 01:21:34 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Feb 13 01:21:41 2007 Subject: [Histonet] shark work Message-ID: Hi all, is anybody out there doing histology on shark tissue? I recall that there was someone, but I have lost all details. If you are still around could you drop me a line as well as anyone else? bets regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From abright <@t> brightinstruments.com Tue Feb 13 03:10:40 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Feb 13 03:09:12 2007 Subject: [Histonet] PMV Cryomicrotome Message-ID: Dear Chris, I posted the same question as you last week for some other users that could not locate any service support. I did not receive any feedback apart from refrigeration service which is well served by local refrigeration service companies. If I do manage to find anyone I will let you have the information, otherwise you have the ?150000 plus option from the same manufacturer or our 8250 cryostat that will section up to 250 X 110mm and can also be fitted with an anti-roll system for very much less money. Please let me know if you find a service company. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: CL Goodall, Anatomy [mailto:Chris.Goodall@bristol.ac.uk] Sent: 12 February 2007 14:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PMV Cryomicrotome Hi histonetters, does anyone know of a service engineer for the PMV450 whole body cryomicrotome? PMV were taken over by LKB, who were subsequently taken over by Leica and they no longer service PMV equipment. Any info would be gratefully received. ---------------------- CL Goodall, Anatomy Chris.Goodall@bristol.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Tue Feb 13 08:33:51 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Feb 13 08:34:22 2007 Subject: [Histonet] rabbit noggin antibody Message-ID: <021320071433.15952.45D1CC4F000409C200003E5022070209539D09020704040A0105@comcast.net> Caroline, I realize you asked about anti-rabbit noggin but 2 professional lives ago, I used a great anti-mouse noggin for both Westerns and IHC. From R&D Systems it was a goat anti-mouse noggin. Worked well in my Westerns down to a few ng detection. Since it worked well under both denatured and non-denaturing conditions, thought of it for IHC. Indeed it worked in frozen and also paraffin with retrieval. Just went after it with an anti-goat secondary pre-absorbed to mouse. Mammalian noggin is nearly homologous to even something like Xenopus noggin and indeed since many embryonic spacial-fate specification molecules are conserved across species, the rabbit noggin target (I never attempted)should be quite similar to mouse noggin (I did successfully in Western and IHC). If no one answers specifically regarding rabbit noggin antibodies, you might give the R&D Systems goat anti-mouse noggin a try. Seems like a logical and reasonable attempt to me. Raymond Koelling Currently Employment Challenged Seattle, WA -------------- Original message -------------- From: Caroline Bass > Hello, > > Can anyone recommend a good antibody for noggin? It will be used for > western blot and staining in rabbit tissues. > > Thanks, > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Feb 13 08:40:47 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Feb 13 08:41:02 2007 Subject: [Histonet] rabbit noggin antibody In-Reply-To: References: Message-ID: No, but I do have a book about Noggin the Nog, from my youth.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: 13 February 2007 02:16 To: histonet@pathology.swmed.edu Subject: [Histonet] rabbit noggin antibody Hello, Can anyone recommend a good antibody for noggin? It will be used for western blot and staining in rabbit tissues. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wiscarrow <@t> serha.ca Tue Feb 13 08:42:11 2007 From: wiscarrow <@t> serha.ca (Scarrow, William (R1SE)) Date: Tue Feb 13 08:43:18 2007 Subject: [Histonet] UNSUBSCRIBE Message-ID: <2BCC0130EEF44B47BDE1B9715C1D56B081081C@RHAEX1.RHA-RRS.CA> ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain priviledged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From koellingr <@t> comcast.net Tue Feb 13 08:51:06 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Feb 13 08:51:18 2007 Subject: [Histonet] PD-L1 in paraffin Message-ID: <021320071451.13308.45D1D05A000724B3000033FC22070209539D09020704040A0105@comcast.net> Mayra, While it never hurts to ask, I'm not optimistic that you will find one yet. My work with B7-H1 (PD-L1, newer nomenclature CD274) was always in frozen or non-parrafin tissue format like cell culture monolayers. I used several clones, always unsuccessful with attempts at localization in formalin fixed paraffin. Indeed if you survey the world literature, all others are unsuccessful. A big urology study in 2005 looking at B7-H1 in Renal Cell Carcinoma states that patient tissue must be fresh frozen as no clones are available for archived paraffin blocks even though attempts to raise such antibodies are underway. Many other studies over 2005-2006 to study expression in various human neoplasms, all say same thing. Use frozen because current antibodies don't work in paraffin. Obviously, the immunogenically dominant epitopes of that molecule are spatial in some way and just don't stand up to any kind of fixation, processing, retrieval. Surely someone, somewhere, sometime will make CD274 antibodies that work in parrafin, but if so it is an extremely recent and current development. Good luck. Ray Koelling Currently Employment Challenged Seattle, WA -------------- Original message -------------- From: macantu@mdanderson.org > Dear Histonetters, > > This is my first time using this histosearch email. I just discovered it > a few weeks ago. My lab is wanting to start looking for B7-H1 (PD-L1) in > human tumor tissues that were fixed in paraffin. I have this working in > frozen tissue ( HRP detection kit from BD) but haven't been able to get it > to work in paraffin. And I cant find a commercially available antibody > that says it works in paraffin. Does anyone know of a commercially > available B7-H1 (PD-L1) antibody for human paraffin tissues? > > Thank you in advance, > > Mayra > > Research Assistant II > Dept. of Melanoma Medical Oncology > MD Anderson Cancer Center > Houston, Texas > Tel. 713-563-9177 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From galinadeyneko <@t> yahoo.com Tue Feb 13 10:03:20 2007 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Feb 13 10:03:37 2007 Subject: [Histonet] Re: Animal tissues. Message-ID: <17764.26169.qm@web33113.mail.mud.yahoo.com> Dear Colleagues. A also would like to share my positive experience for softening animal tissues with glycerin solution. I worked with mouse,rat, rabbit and monkeys. I do not use exact percent solution, but let say 1 part of glycerol, 1part of soft liquid soap and 2 parts of distilled water. Usually, I wet the several layers of paper towel with this solution, placed on flat tray or cold plate, place the trimmed blocks upside down on the wet towel and place a tray at 4C overnight. Of cause, be careful with first section, tissue might be swelling. I have no problems with IHC or standard staining methods. In Russia we made the old archive formalin fixed specimens more soft by placing them in castor oil overnight before processing (we did processing manually in the ovens 37 C and 56 C), but I am not sure, that such method is suitable for research. Many thanks for James Watson for the idea with glycerin, because I had a lot of problems with rodent hearts. best regards. Galina Deyneko. Novartis,Cambridge MA, 617-871-7613 --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From rjbuesa <@t> yahoo.com Tue Feb 13 10:49:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 13 10:49:36 2007 Subject: [Histonet] interaction of techs with pathologist assistants In-Reply-To: Message-ID: <109736.87704.qm@web61211.mail.yahoo.com> The assistance varies from place to place. From 3 institutions I can talk about here is how it was done: 1- the techs did the cassetting from specimens selected and left in jars by the PA (hours after that selection took place); 2- the PAs did everything, including cassetting and placing them into the baskets (only the baskets were handled by the techs); and 3- a tech worked side by side with the PA and prepared the cassettes the moment the grossing was completed. The most efficient way is in "case 2". Ren? J. "Vickroy, Jim" wrote: I would appreciate information from other institutions on how much technical assistance a pathologist assistant requires or gets when doing grossing of specimens. Our pa's handle most of the larger specimens and many of my techs think that the pa's need more assistance here than at other institutions. I realize that duties and expectations probably vary but I am trying to get a handle on usual practices. Most of us have been around before pa's existed and have always assisted pathologists but are somewhat vague on how much help a pa should have. I would appreciate any information you would have. Currently we having techs grossing small surgicals by themselves and assisting pa's with the larger surgical specimens. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From rjbuesa <@t> yahoo.com Tue Feb 13 10:52:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 13 10:55:58 2007 Subject: [Histonet] HPV In-Reply-To: <6FF91AE4F1DC7743A6466E334EB865AE0BE0F7F6@VERITY.roswellpark.org> Message-ID: <607139.55075.qm@web61217.mail.yahoo.com> I always used with success the Dako Rabbit Ab at a dilution of 1:75 and HIER at pH6 Ren? J. "Vaughan, Mary" wrote: Hi everyone - I was hoping some of you... my clinical friends, could recommend a good HPV antibody. I'm in research and have a human project that requires me to do this Ab. Thanks. Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From histotech <@t> hotmail.com Tue Feb 13 10:59:45 2007 From: histotech <@t> hotmail.com (Pam Wirth) Date: Tue Feb 13 10:59:59 2007 Subject: [Histonet] shandon laser microwriter Message-ID: Dear Histonetters We are selling our Shandon Laser Microwriter due to space limitations. It is capable of printing 3 slides per second or one slide per second if using the barcode system. Unfortunately our lab is 800sq ft and we process less than 50 blocks a day on average. This is a great instrument for high volume labs with enough space. The thermoshandon website lists more detailed information. Due to its size it may be best for a local laboratory (we are in Nashville, TN). Thanks Pam Wirth, Laboratory Manager Immunohistochemistry Core Laboratory Vanderbilt University Nashville, TN _________________________________________________________________ >From predictions to trailers, check out the MSN Entertainment Guide to the Academy Awards® http://movies.msn.com/movies/oscars2007/?icid=ncoscartagline1 From JEllin <@t> yumaregional.org Tue Feb 13 11:17:30 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Feb 13 11:33:06 2007 Subject: [Histonet] interaction of techs with pathologist assistants Message-ID: <29BE166A2CF48D459853F8EC57CD37E88A06A8@EXCHANGECLUSTER.yumaregional.local> Speaking from experience, doing the gross, I like to make my own cassettes. This allows me the security that I am the one writing the number on the case and that there is no discrepancy with this. Secondly we currently are completely automated which makes things even better I order my own cassettes on every case and also put them into the basket so they are lined up by numberical oreder as well as in order of priorty. This helps the techs in the morning with cutting. But I have to agree with Renee, route 2 is better. The only people that get any help at the gross room is the Pathologist, but they are even starting to use the computer to order cassettes while grossing. IF you have any questions please feel free to give me a call. Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 13, 2007 9:49 AM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interaction of techs with pathologist assistants The assistance varies from place to place. From 3 institutions I can talk about here is how it was done: 1- the techs did the cassetting from specimens selected and left in jars by the PA (hours after that selection took place); 2- the PAs did everything, including cassetting and placing them into the baskets (only the baskets were handled by the techs); and 3- a tech worked side by side with the PA and prepared the cassettes the moment the grossing was completed. The most efficient way is in "case 2". Ren? J. "Vickroy, Jim" wrote: I would appreciate information from other institutions on how much technical assistance a pathologist assistant requires or gets when doing grossing of specimens. Our pa's handle most of the larger specimens and many of my techs think that the pa's need more assistance here than at other institutions. I realize that duties and expectations probably vary but I am trying to get a handle on usual practices. Most of us have been around before pa's existed and have always assisted pathologists but are somewhat vague on how much help a pa should have. I would appreciate any information you would have. Currently we having techs grossing small surgicals by themselves and assisting pa's with the larger surgical specimens. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From deb.vaneyck <@t> phci.org Tue Feb 13 12:35:43 2007 From: deb.vaneyck <@t> phci.org (Van Eyck, Deb) Date: Tue Feb 13 12:37:36 2007 Subject: [Histonet] tissue cassettes with pegs In-Reply-To: <200702131800.l1DI0KFK007280@mx.phci.org> Message-ID: Hello there, Is anyone aware of an embedding cassette that has small pegs? in it for alignment of tissue sections or cores?? Someone told me there is one available? If so please share the name and company selling. Thanks Deb This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From opiecurt <@t> yahoo.com Tue Feb 13 12:50:38 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Tue Feb 13 12:50:48 2007 Subject: [Histonet] anyone looking for work in So. Cal? Message-ID: <46631.91324.qm@web81610.mail.mud.yahoo.com> We may be looking to add another tech or two in the next month, anyone looking for a job? reply to the email first and we'll go from there. curt --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From Derek.Papalegis <@t> tufts.edu Tue Feb 13 13:12:10 2007 From: Derek.Papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Feb 13 13:12:18 2007 Subject: [Histonet] Hard tissue network Message-ID: <20070213141210.3whpb80n44oock4c@webmail.tufts.edu> Hi All, I will soon be working with hard tissues and cutting plastics. I was told that through NSH, I should sign up for the Hard Tissue Network as well as the VIR network. I have been unable to find these anywhere. Does anyone know anything about these 2 resources and how I can join them? Thanks, Derek From Annette_hall <@t> pa-ucl.com Tue Feb 13 13:32:00 2007 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Tue Feb 13 13:32:25 2007 Subject: [Histonet] Tissue Microarray tools Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5AB8@mercury.pa-ucl.com> Can anyone recommend a good system for making tissue microarrays. We are not a large volume lab (approx 250 IHC per month), so I would be looking at manual methods for accomplishing this. My planned uses would be for new antibody evaluations and controls. Thanks, Annette Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tp2 <@t> medicine.wisc.edu Tue Feb 13 13:39:54 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Feb 13 13:40:13 2007 Subject: [Histonet] Tissue Microarray tools Message-ID: <45D1BFAA020000DF00004F0C@gw.medicine.wisc.edu> Annette, You might want to look into Beecher Instruments. I use their manual arrayer and have been happy with it so far. http://www.beecherinstruments.com/index.html Tom Pier >>> Annette Hall 02/13/07 1:32 PM >>> Can anyone recommend a good system for making tissue microarrays. We are not a large volume lab (approx 250 IHC per month), so I would be looking at manual methods for accomplishing this. My planned uses would be for new antibody evaluations and controls. Thanks, Annette Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Feb 13 14:58:06 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Feb 13 14:58:34 2007 Subject: [Histonet] Slide printer Message-ID: Hi all. Am desperate for info on slide printers. Currently have one from Sakura that has broken down (again). We would be printing 300-500 slides a day with path #, Antibody names, possible more info on slides. Need something fast and reliable. Thanks so much. Hate to pay registered techs to write up these slides! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From AGrobe2555 <@t> aol.com Tue Feb 13 15:55:41 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Feb 13 15:56:01 2007 Subject: [Histonet] Re:Tissue arrays Message-ID: Annette, The journal of Histotechnology Vol 14 #2 of June 1991 has an article entitled "Parallel Array Method of Embedding Multiple Tissue Samples in a Single Paraffin Block" by True et.al. This might work for you, or at least get you going.... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From rfisher <@t> gbmc.org Wed Feb 14 07:58:45 2007 From: rfisher <@t> gbmc.org (RENEE FISHER) Date: Wed Feb 14 07:59:15 2007 Subject: [Histonet] Special Stainers Message-ID: <45D2CF44.09C2.00CA.0@gbmc.org> Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From doug <@t> ppspath.com Wed Feb 14 08:21:38 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Feb 14 08:23:06 2007 Subject: [Histonet] Special Stainers In-Reply-To: <45D2CF44.09C2.00CA.0@gbmc.org> Message-ID: Renee, Contact Ventana about the NexES special stainer. Toll Free (800) 227-2155 Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ____________________________________________________________________________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From roosmith1 <@t> hotmail.com Wed Feb 14 09:07:43 2007 From: roosmith1 <@t> hotmail.com (Jackie Smith) Date: Wed Feb 14 09:07:59 2007 Subject: [Histonet] Special Stainers In-Reply-To: Message-ID: I would additionally contact someone from Dako regarding their Artisan Special Stainer. Their stain selection as well as the quality of stains is far superior to the Nexes...speaking from experience as a user of both systems. Dako does offer several different options for leasing, RAA or purchasing. I'm sure both vendors would bring theri systems in to do a side by side demo. We were able to keep our unit for a 60 day demo/evaluation. Good Luck, Eric Smith Wilmington Pathology Associates ______________________________________________________________ From: "Douglas D Deltour" To: "'RENEE FISHER'" , Subject: RE: [Histonet] Special Stainers Date: Wed, 14 Feb 2007 09:21:38 -0500 Renee, Contact Ventana about the NexES special stainer. Toll Free (800) 227-2155 Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ___________________________________________________________________ _________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]From predictions to trailers, check out the MSN Entertainment Guide to the Academy Awards® References 1. http://g.msn.com/8HMBENUS/2752??PS=47575 From ryakay <@t> shands.ufl.edu Wed Feb 14 09:08:52 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Wed Feb 14 09:09:42 2007 Subject: [Histonet] Special Stainers In-Reply-To: <45D2CF44.09C2.00CA.0@gbmc.org> References: <45D2CF44.09C2.00CA.0@gbmc.org> Message-ID: <45D2DFB4020000D80000087D@gw-fs1.shands.ufl.edu> We have 2 of the Dako Artisan special stainers and love them.. they can be reached at 800-235-5743. Kaye Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> "RENEE FISHER" 2/14/2007 8:58 AM >>> Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Feb 14 09:57:18 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Wed Feb 14 09:57:39 2007 Subject: [Histonet] Special Stainers In-Reply-To: <45D2CF44.09C2.00CA.0@gbmc.org> Message-ID: <200702141557.l1EFvFW0089962@pro12.abac.com> I would vote for the Dako Artisan as well. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ____________________________________________________________________________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgolbaba <@t> uoguelph.ca Wed Feb 14 10:09:22 2007 From: mgolbaba <@t> uoguelph.ca (mgolbaba@uoguelph.ca) Date: Wed Feb 14 10:09:32 2007 Subject: [Histonet] Permanent microscopic slide Message-ID: <20070214110922.82u5tcsrkgo8oosw@webmail.uoguelph.ca> Hello everyone, I am interested in preparing permanent microscopic slides of my plant specimens (size = nm). Would you let me know about its method or any necessary info? Thanks a lot. Mahsa ----------------------------- Mahsa Golbabaie MSc Student, Department of Plant Agriculture, University of Guelph, Canada Department of Chemical Engineering, University of Waterloo, Canada Email: mgolbaba@uoguelph.ca mgolbaba@engmail.uwaterloo.ca From Paula.F.Conlon <@t> Lahey.org Wed Feb 14 10:13:25 2007 From: Paula.F.Conlon <@t> Lahey.org (Conlon, Paula F.) Date: Wed Feb 14 10:13:32 2007 Subject: [Histonet] surgical brain specimens Message-ID: Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites= , staff, services and career opportunities.=0A= =0A= THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED.= IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM= DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, you= r use of this message for any purpose is strictly prohibited. If you have re= ceived this communication in error, please delete the message and notify the= sender so that we may correct our records. From JWEEMS <@t> sjha.org Wed Feb 14 10:10:38 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Feb 14 10:15:08 2007 Subject: [Histonet] Special Stainers Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33BC@sjhaexc02.sjha.org> Me too!!! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of patsy ruegg Sent: Wednesday, February 14, 2007 10:57 AM To: 'RENEE FISHER'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Special Stainers I would vote for the Dako Artisan as well. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ____________________________________________________________________________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Feb 14 10:16:29 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Feb 14 10:17:04 2007 Subject: [Histonet] surgical brain specimens Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33BF@sjhaexc02.sjha.org> My experience has always been that brain works beeter if you can air dry the sections before heat drying, even with charged slides. If you have time to do this you might want to air dry overnight, then continue with your usual procedure. Good luck, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Conlon, Paula F. Sent: Wednesday, February 14, 2007 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surgical brain specimens Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Feb 14 10:20:01 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Feb 14 10:20:29 2007 Subject: [Histonet] surgical brain specimens Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33C1@sjhaexc02.sjha.org> Oops - that would be BETTER!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, February 14, 2007 11:16 AM To: Conlon, Paula F.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surgical brain specimens My experience has always been that brain works beeter if you can air dry the sections before heat drying, even with charged slides. If you have time to do this you might want to air dry overnight, then continue with your usual procedure. Good luck, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Conlon, Paula F. Sent: Wednesday, February 14, 2007 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surgical brain specimens Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jmahoney <@t> alegent.org Wed Feb 14 10:22:24 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Feb 14 10:22:41 2007 Subject: [Histonet] surgical brain specimens In-Reply-To: References: Message-ID: <45D2E2E00200003C0000525D@gwia.alegent.org> The first and probably most obvious would be that if you are using an adhesive in your waterbath you should not use it with plus slides. The second would be to let your sections dry at room temp for a while before putting gin the oven. Hope this gives you a place to start. Jan Omaha >>> "Conlon, Paula F." 02/14/2007 10:13 AM >>> Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Wed Feb 14 10:25:35 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Feb 14 10:29:50 2007 Subject: [Histonet] Special Stainers In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33BC@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33BC@sjhaexc02.sjha.org> Message-ID: <45D2E39F0200003C00005267@gwia.alegent.org> We have tried several and my techs keep preferring Ventana's Nexis. It is so easy to set up and really is walk away. The only down side is that it is not continuous feed but the runs are relatively short and we can do many runs a day if needed. Jan Omaha >>> "Weems, Joyce" 02/14/2007 10:10 AM >>> Me too!!! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of patsy ruegg Sent: Wednesday, February 14, 2007 10:57 AM To: 'RENEE FISHER'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Special Stainers I would vote for the Dako Artisan as well. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ____________________________________________________________________________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jmahoney <@t> alegent.org Wed Feb 14 10:31:02 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Feb 14 10:31:19 2007 Subject: [Histonet] surgical brain specimens In-Reply-To: <45D2E2E00200003C0000525D@gwia.alegent.org> References: <45D2E2E00200003C0000525D@gwia.alegent.org> Message-ID: <45D2E4E60200003C00005284@gwia.alegent.org> OOOPS for me too. I don't like my gin in the oven, I prefer it with tonic over ice. >>> "Janice Mahoney" 02/14/2007 10:22 AM >>> The first and probably most obvious would be that if you are using an adhesive in your waterbath you should not use it with plus slides. The second would be to let your sections dry at room temp for a while before putting gin the oven. Hope this gives you a place to start. Jan Omaha >>> "Conlon, Paula F." 02/14/2007 10:13 AM >>> Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wqi <@t> biocare.net Wed Feb 14 10:33:34 2007 From: wqi <@t> biocare.net (Weimin Qi) Date: Wed Feb 14 10:37:00 2007 Subject: [Histonet] Astrocytoma slides In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33C1@sjhaexc02.sjha.org> Message-ID: <20070214163254.ONYK9790.imta01a2.registeredsite.com@Wqi> Does any one know a good source to get astrocytoma slides? Thank you in advance. Weimin From portera <@t> msu.edu Wed Feb 14 10:40:57 2007 From: portera <@t> msu.edu (Amy Porter) Date: Wed Feb 14 10:41:09 2007 Subject: [Histonet] surgical brain specimens References: Message-ID: <002401c75056$e705e040$8e7a0923@histolab> Paula - it would definately slow your TAT - here at MSU we used to house the Mid Michigan Dementia Network. We sectioned all of our well fixede routinely processed brains at the appropriate thickness for the stain to be applied and ALWAYS let them air dry completely overnight before placing in drying oven. This usually gave us about a 95% success rate for section retention. Hope this helps out. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Conlon, Paula F." To: Sent: Wednesday, February 14, 2007 11:13 AM Subject: [Histonet] surgical brain specimens Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Feb 14 10:38:45 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Feb 14 10:42:53 2007 Subject: [Histonet] Special Stainers Message-ID: Renee, The Artisan sold by Dako produces great results. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 7:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 14 10:44:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 14 10:51:14 2007 Subject: [Histonet] Permanent microscopic slide In-Reply-To: <20070214110922.82u5tcsrkgo8oosw@webmail.uoguelph.ca> Message-ID: <902777.15249.qm@web61220.mail.yahoo.com> Masha: Such a broad question would require a broader answer, i.e. a book about plant microscopy, and it would also depend on what are you trying to make permanent slides from. The size you specify, is it "nm" = nanometer or was it a "wondering" finger trying to write "mm" = millimeter? The approach would be completelly different. If your plant material is in the "mm" (or even "nm" size range) you would have to go with the classical methods used for diatoms and use a water soluble medium and seal the coverslip (ideally round) with a liquid sealant that will harden when dried. You could try to dehydrate and "clear" your material and for that purpose the drying and clearing (i.e. subject the material to an agent miscible with the final anhydrous mounting medium) will have to be done in centrifuge tubes and the process carried out by centrifugation that will assure to keep the material and not lose it while being transferred from one step to the next. If this is what you are going to do, after each centrifugation use a dropper to remove the medium (which ever it is for the step) and add the next, until you get to the final one when you are going to extract as much as possible and with the same dropper get a drop with your specimens, place then in a slide, add the mounting medium, either Canada balsam (even if this sound too old fashioned), of resin of Damar or Permount. Cover with the coverslip afterwards, remove the excess around the coverslip and let it dry (the drying time depending on the medium). Good luck with your permanent slides! Ren? J. "mgolbaba@uoguelph.ca" wrote: Hello everyone, I am interested in preparing permanent microscopic slides of my plant specimens (size = nm). Would you let me know about its method or any necessary info? Thanks a lot. Mahsa ----------------------------- Mahsa Golbabaie MSc Student, Department of Plant Agriculture, University of Guelph, Canada Department of Chemical Engineering, University of Waterloo, Canada Email: mgolbaba@uoguelph.ca mgolbaba@engmail.uwaterloo.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From rjbuesa <@t> yahoo.com Wed Feb 14 10:57:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 14 10:58:01 2007 Subject: [Histonet] surgical brain specimens In-Reply-To: Message-ID: <348626.51157.qm@web61223.mail.yahoo.com> Paula: You ask about better sections or better retention? We used to section brain at more than 5 ?m, specially if there was the idea of silver staining for Alzheimer's; 5 ?m are too thin to visualize the tangles. For retention we always stained the slides the next day they were cut. The slides were placed vertical on the smaller side for about 1 hour before being put in the trays, that were left outside the oven during the night. The next day they were placed in the oven at 60?C for 30 minutes before being stained (either routine ot HC). Ren? J. "Conlon, Paula F." wrote: Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From akemiat3377 <@t> yahoo.com Wed Feb 14 11:15:45 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Feb 14 11:15:56 2007 Subject: [Histonet] Special Stainers In-Reply-To: <45D2E39F0200003C00005267@gwia.alegent.org> Message-ID: <641260.60275.qm@web31303.mail.mud.yahoo.com> How are the VVG (Elastic) stains on the Ventana? Are the elastic fibers lavender or black? Also, the amyloid stains? Akemi Allison-Tacha Janice Mahoney wrote: We have tried several and my techs keep preferring Ventana's Nexis. It is so easy to set up and really is walk away. The only down side is that it is not continuous feed but the runs are relatively short and we can do many runs a day if needed. Jan Omaha >>> "Weems, Joyce" 02/14/2007 10:10 AM >>> Me too!!! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of patsy ruegg Sent: Wednesday, February 14, 2007 10:57 AM To: 'RENEE FISHER'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Special Stainers I would vote for the Dako Artisan as well. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ____________________________________________________________________________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CCross6032 <@t> aol.com Wed Feb 14 11:16:59 2007 From: CCross6032 <@t> aol.com (CCross6032@aol.com) Date: Wed Feb 14 11:17:19 2007 Subject: [Histonet] caspase 3 and background staining Message-ID: Hi everyone - I see that there are several successful caspase 3 protocols in rodents running; my question is - in my control tissues the respiratory epithelium, and interstitial cells of the lung (they look like histiocytes microscopically) light up; i am looking primarily at the heart and there is an acceptable level of background for interpretation (in my opinion anyway :) ). I have done searches and cannot find if these are normal regions to light up - if so, why? we are using the active caspase 3 antibody and the Dako envision system. any references as to the normal caspase 3 positive cells would be great! thank you! Cheryl Cross UTCVM From cmiller <@t> physlab.com Wed Feb 14 11:28:15 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Feb 14 11:32:19 2007 Subject: [Histonet] Special Stainers In-Reply-To: <641260.60275.qm@web31303.mail.mud.yahoo.com> References: <45D2E39F0200003C00005267@gwia.alegent.org> <641260.60275.qm@web31303.mail.mud.yahoo.com> Message-ID: <001201c7505d$829f0a30$db01a8c0@plab.local> Love the Ventana....I used it and maintained it for over 4 years and I never had a problem. I didn't like the dako, to much time to set it up. Ventana is load and Go. We can do 3 runs per shift if need be. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, February 14, 2007 11:16 AM To: Janice Mahoney Cc: histonet Subject: RE: [Histonet] Special Stainers How are the VVG (Elastic) stains on the Ventana? Are the elastic fibers lavender or black? Also, the amyloid stains? Akemi Allison-Tacha Janice Mahoney wrote: We have tried several and my techs keep preferring Ventana's Nexis. It is so easy to set up and really is walk away. The only down side is that it is not continuous feed but the runs are relatively short and we can do many runs a day if needed. Jan Omaha >>> "Weems, Joyce" 02/14/2007 10:10 AM >>> Me too!!! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of patsy ruegg Sent: Wednesday, February 14, 2007 10:57 AM To: 'RENEE FISHER'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Special Stainers I would vote for the Dako Artisan as well. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ____________________________________________________________________________ ___________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From awatanabe <@t> tgen.org Wed Feb 14 11:31:27 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Wed Feb 14 11:35:37 2007 Subject: [Histonet] Re: Tissue Microarray tools In-Reply-To: <20070214164817.1B1182005017@mr1.tgen.org> Message-ID: I use the arrayer originally sold by Chemicon but is now sold by EDM. I also work at a facility where we make TMAs for other labs such as your self for a fee. We construct the TMAs using your own blocks and a few of ours to fill out the control section. We then cut the TMA for you and dip them in paraffin for storage if you wish. You can find our facility information at www.tgen.org. Look for the Tissue Microarray Facility on the website. You can also contact me at this address and I can give you further information. On 2/14/07 9:48 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. tissue cassettes with pegs (Van Eyck, Deb) > 2. anyone looking for work in So. Cal? (curt tague) > 3. Hard tissue network (Derek Papalegis) > 4. Tissue Microarray tools (Annette Hall) > 5. Re: Tissue Microarray tools (Thomas Pier) > 6. Slide printer (Patti Loykasek) > 7. Re:Tissue arrays (AGrobe2555@aol.com) > 8. Special Stainers (RENEE FISHER) > 9. RE: Special Stainers (Douglas D Deltour) > 10. RE: Special Stainers (Jackie Smith) > 11. Re: Special Stainers (Kaye Ryan) > 12. RE: Special Stainers (patsy ruegg) > 13. Permanent microscopic slide (mgolbaba@uoguelph.ca) > 14. surgical brain specimens (Conlon, Paula F.) > 15. RE: Special Stainers (Weems, Joyce) > 16. RE: surgical brain specimens (Weems, Joyce) > 17. RE: surgical brain specimens (Weems, Joyce) > 18. Re: surgical brain specimens (Janice Mahoney) > 19. RE: Special Stainers (Janice Mahoney) > 20. Re: surgical brain specimens (Janice Mahoney) > 21. Astrocytoma slides (Weimin Qi) > 22. Re: surgical brain specimens (Amy Porter) > 23. RE: Special Stainers (Dawson, Glen) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 13 Feb 2007 12:35:43 -0600 > From: "Van Eyck, Deb" > Subject: [Histonet] tissue cassettes with pegs > To: > Message-ID: > > Content-Type: text/plain; charset=iso-8859-1 > > Hello there, > > Is anyone aware of an embedding cassette that has small pegs? in it for > alignment of tissue sections or cores?? Someone told me there is one > available? If so please share the name and company selling. Thanks Deb > > > This information is confidential and intended solely for the use of the > individual or entity to whom it is addressed. If you have received this email > in error please notify the sender or our Customer Support Center at (262) > 928-2777. We have scanned this e-mail and its attachments for malicious > content. However, the recipient should check this email and any attachments > for the presence of viruses. ProHealth Care accepts no liability for any > damage caused by any virus transmitted by this email. > > > > ------------------------------ > > Message: 2 > Date: Tue, 13 Feb 2007 10:50:38 -0800 (PST) > From: curt tague > Subject: [Histonet] anyone looking for work in So. Cal? > To: histonet@lists.utsouthwestern.edu > Message-ID: <46631.91324.qm@web81610.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > We may be looking to add another tech or two in the next month, anyone looking > for a job? > reply to the email first and we'll go from there. > > curt > > > --------------------------------- > Don't pick lemons. > See all the new 2007 cars at Yahoo! Autos. > > ------------------------------ > > Message: 3 > Date: Tue, 13 Feb 2007 14:12:10 -0500 > From: Derek Papalegis > Subject: [Histonet] Hard tissue network > To: histonet@lists.utsouthwestern.edu > Message-ID: <20070213141210.3whpb80n44oock4c@webmail.tufts.edu> > Content-Type: text/plain; charset=ISO-8859-1; format="flowed" > > Hi All, > I will soon be working with hard tissues and cutting plastics. I was > told that through NSH, I should sign up for the Hard Tissue Network as > well as the VIR network. I have been unable to find these anywhere. > Does anyone know anything about these 2 resources and how I can join > them? > > Thanks, > > Derek > > > > > ------------------------------ > > Message: 4 > Date: Tue, 13 Feb 2007 13:32:00 -0600 > From: Annette Hall > Subject: [Histonet] Tissue Microarray tools > To: histonet@lists.utsouthwestern.edu > Message-ID: > <9FC023A4AB52BB4D87DC6456081A822C012B5AB8@mercury.pa-ucl.com> > Content-Type: text/plain; charset="iso-8859-1" > > > Can anyone recommend a good system for making tissue microarrays. We are not > a large volume lab (approx 250 IHC per month), so I would be looking at > manual methods for accomplishing this. My planned uses would be for new > antibody evaluations and controls. > > Thanks, Annette > > Annette J Hall, MT > Micro/Cyto/Histo Supervisor > United Clinical Labs > 205 Bluff Street > Dubuque, IA 52001 > Phone: 563.556.2010 > Cell: 563.580.9751 > Fax: 563.584.2085 > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > ------------------------------ > > Message: 5 > Date: Tue, 13 Feb 2007 13:39:54 -0600 > From: "Thomas Pier" > Subject: Re: [Histonet] Tissue Microarray tools > To: , > Message-ID: <45D1BFAA020000DF00004F0C@gw.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Annette, > You might want to look into Beecher Instruments. I use their manual arrayer > and have been happy with it so far. > > http://www.beecherinstruments.com/index.html > > Tom Pier > >>>> Annette Hall 02/13/07 1:32 PM >>> > > Can anyone recommend a good system for making tissue microarrays. We are not > a large volume lab (approx 250 IHC per month), so I would be looking at > manual methods for accomplishing this. My planned uses would be for new > antibody evaluations and controls. > > Thanks, Annette > > Annette J Hall, MT > Micro/Cyto/Histo Supervisor > United Clinical Labs > 205 Bluff Street > Dubuque, IA 52001 > Phone: 563.556.2010 > Cell: 563.580.9751 > Fax: 563.584.2085 > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Tue, 13 Feb 2007 12:58:06 -0800 > From: Patti Loykasek > Subject: [Histonet] Slide printer > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi all. Am desperate for info on slide printers. Currently have one from > Sakura that has broken down (again). We would be printing 300-500 slides a > day with path #, Antibody names, possible more info on slides. Need > something fast and reliable. Thanks so much. Hate to pay registered techs to > write up these slides! > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > ------------------------------ > > Message: 7 > Date: Tue, 13 Feb 2007 16:55:41 EST > From: AGrobe2555@aol.com > Subject: [Histonet] Re:Tissue arrays > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Annette, > The journal of Histotechnology Vol 14 #2 of June 1991 has an article > entitled "Parallel Array Method of Embedding Multiple Tissue Samples in a > Single > Paraffin Block" by True et.al. This might work for you, or at least get you > going.... > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > ------------------------------ > > Message: 8 > Date: Wed, 14 Feb 2007 08:58:45 -0500 > From: "RENEE FISHER" > Subject: [Histonet] Special Stainers > To: > Message-ID: <45D2CF44.09C2.00CA.0@gbmc.org> > Content-Type: text/plain; charset=US-ASCII > > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ______________________________________________________________________________ > _________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > > > ------------------------------ > > Message: 9 > Date: Wed, 14 Feb 2007 09:21:38 -0500 > From: "Douglas D Deltour" > Subject: RE: [Histonet] Special Stainers > To: "'RENEE FISHER'" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Renee, > > Contact Ventana about the NexES special stainer. Toll Free (800) 227-2155 > > > Douglas D. Deltour HT(ASCP) > Histology Supervisor > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER > Sent: Wednesday, February 14, 2007 8:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Stainers > > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ____________________________________________________________________________ > ___________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > Message: 10 > Date: Wed, 14 Feb 2007 10:07:43 -0500 > From: "Jackie Smith" > Subject: RE: [Histonet] Special Stainers > To: doug@ppspath.com, rfisher@gbmc.org, > histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > I would additionally contact someone from Dako regarding their Artisan > Special Stainer. Their stain selection as well as the quality of > stains is far superior to the Nexes...speaking from experience as a > user of both systems. > > Dako does offer several different options for leasing, RAA or > purchasing. I'm sure both vendors would bring theri systems in to do a > side by side demo. We were able to keep our unit for a 60 day > demo/evaluation. > > Good Luck, > > Eric Smith > > Wilmington Pathology Associates > ______________________________________________________________ > > From: "Douglas D Deltour" > To: "'RENEE FISHER'" > , > Subject: RE: [Histonet] Special Stainers > Date: Wed, 14 Feb 2007 09:21:38 -0500 > Renee, > Contact Ventana about the NexES special stainer. Toll Free (800) > 227-2155 > Douglas D. Deltour HT(ASCP) > Histology Supervisor > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or > entity to > which it is addressed and may contain information that is > privileged, > confidential and exempt from disclosure under applicable law. If > the reader > of this message is not the intended recipient, you are hereby > notified that > any dissemination, distribution, or copying of this communication > is > strictly prohibited by law. If you have received this communication > in > error, please notify me immediately. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > RENEE FISHER > Sent: Wednesday, February 14, 2007 8:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Stainers > Greetings All- > We are considering a reagent rental on an automatic special > stainer. > Does anyone have a good experience with a particular instrument and > if > so can we get the contact information? > Thanks > ___________________________________________________________________ > _________ > ___________ > This email may contain confidential protected health information > and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party > unless > required to do so by law. If you are not the intended recipient, > you are > hereby notified that any disclosure, copying, distribution, or > action > taken in reliance on the contents of this email is strictly > prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > > [1]From predictions to trailers, check out the MSN Entertainment Guide > to the Academy Awards? > > References > > 1. http://g.msn.com/8HMBENUS/2752??PS=47575 > > > ------------------------------ > > Message: 11 > Date: Wed, 14 Feb 2007 10:08:52 -0500 > From: "Kaye Ryan" > Subject: Re: [Histonet] Special Stainers > To: "RENEE FISHER" , > > Message-ID: <45D2DFB4020000D80000087D@gw-fs1.shands.ufl.edu> > Content-Type: text/plain; charset=US-ASCII > > We have 2 of the Dako Artisan special stainers and love them.. they can be > reached at 800-235-5743. > > Kaye > > Kaye Ryan > Histology Manager/Educational Coordinator > Shands Rocky Point Laboratories > (352) 265-0111, 72093 > >>>> "RENEE FISHER" 2/14/2007 8:58 AM >>> > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ______________________________________________________________________________ > _________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 12 > Date: Wed, 14 Feb 2007 08:57:18 -0700 > From: "patsy ruegg" > Subject: RE: [Histonet] Special Stainers > To: "'RENEE FISHER'" , > > Message-ID: <200702141557.l1EFvFW0089962@pro12.abac.com> > Content-Type: text/plain; charset="us-ascii" > > I would vote for the Dako Artisan as well. > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER > Sent: Wednesday, February 14, 2007 6:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Stainers > > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ____________________________________________________________________________ > ___________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Wed, 14 Feb 2007 11:09:22 -0500 > From: "mgolbaba@uoguelph.ca" > Subject: [Histonet] Permanent microscopic slide > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20070214110922.82u5tcsrkgo8oosw@webmail.uoguelph.ca> > Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; > format="flowed" > > Hello everyone, > > I am interested in preparing permanent microscopic slides of my plant > specimens (size = nm). Would you let me know about its method or any > necessary info? > Thanks a lot. > > Mahsa > > > ----------------------------- > Mahsa Golbabaie > MSc Student, > Department of Plant Agriculture, University of Guelph, Canada > Department of Chemical Engineering, University of Waterloo, Canada > Email: mgolbaba@uoguelph.ca > mgolbaba@engmail.uwaterloo.ca > > > > > > > > ------------------------------ > > Message: 14 > Date: Wed, 14 Feb 2007 11:13:25 -0500 > From: "Conlon, Paula F." > Subject: [Histonet] surgical brain specimens > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters, We are receiving more surgical brain sections than we > have in the past. We process the specimens on the Pathos Automatic > Microwave Histoprocessor and we cut the sections at 5 microns. The > sections sometimes do not stay on the slides even though we use Plus > slides. The Neuropathologist is not happy and she has asked me to find > out if there is some way to get better sections. Thank you in advance > for your help. > > Paula Conlon > > Histology Supervisor > > Lahey Clinic > > 41 Mall Road > > Burlington, MA > > 01805 > > 781-744-1345 > > > > > See our web page at http://www.lahey.org for a full directory of Lahey sites= > , staff, services and career opportunities.=0A= > =0A= > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED.= > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM= > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, you= > r use of this message for any purpose is strictly prohibited. If you have re= > ceived this communication in error, please delete the message and notify the= > sender so that we may correct our records. > > > ------------------------------ > > Message: 15 > Date: Wed, 14 Feb 2007 11:10:38 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Special Stainers > To: "RENEE FISHER" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33BC@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Me too!!! > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of patsy > ruegg > Sent: Wednesday, February 14, 2007 10:57 AM > To: 'RENEE FISHER'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Special Stainers > > > I would vote for the Dako Artisan as well. > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER > Sent: Wednesday, February 14, 2007 6:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Stainers > > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ____________________________________________________________________________ > ___________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information is > strictly prohibited. If you have received this communication in error, please > notify us immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 16 > Date: Wed, 14 Feb 2007 11:16:29 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] surgical brain specimens > To: "Conlon, Paula F." , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33BF@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > My experience has always been that brain works beeter if you can air dry the > sections before heat drying, even with charged slides. If you have time to do > this you might want to air dry overnight, then continue with your usual > procedure. > > Good luck, > Joyce > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Conlon, > Paula F. > Sent: Wednesday, February 14, 2007 11:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] surgical brain specimens > > > Hi Histonetters, We are receiving more surgical brain sections than we > have in the past. We process the specimens on the Pathos Automatic > Microwave Histoprocessor and we cut the sections at 5 microns. The > sections sometimes do not stay on the slides even though we use Plus > slides. The Neuropathologist is not happy and she has asked me to find > out if there is some way to get better sections. Thank you in advance > for your help. > > Paula Conlon > > Histology Supervisor > > Lahey Clinic > > 41 Mall Road > > Burlington, MA > > 01805 > > 781-744-1345 > > > > > See our web page at http://www.lahey.org for a full directory of Lahey sites, > staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT > MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify the > sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information is > strictly prohibited. If you have received this communication in error, please > notify us immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 17 > Date: Wed, 14 Feb 2007 11:20:01 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] surgical brain specimens > To: > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32037D33C1@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Oops - that would be BETTER!!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, > Joyce > Sent: Wednesday, February 14, 2007 11:16 AM > To: Conlon, Paula F.; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] surgical brain specimens > > > My experience has always been that brain works beeter if you can air dry the > sections before heat drying, even with charged slides. If you have time to do > this you might want to air dry overnight, then continue with your usual > procedure. > > Good luck, > Joyce > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Conlon, > Paula F. > Sent: Wednesday, February 14, 2007 11:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] surgical brain specimens > > > Hi Histonetters, We are receiving more surgical brain sections than we > have in the past. We process the specimens on the Pathos Automatic > Microwave Histoprocessor and we cut the sections at 5 microns. The > sections sometimes do not stay on the slides even though we use Plus > slides. The Neuropathologist is not happy and she has asked me to find > out if there is some way to get better sections. Thank you in advance > for your help. > > Paula Conlon > > Histology Supervisor > > Lahey Clinic > > 41 Mall Road > > Burlington, MA > > 01805 > > 781-744-1345 > > > > > See our web page at http://www.lahey.org for a full directory of Lahey sites, > staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT > MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify the > sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information is > strictly prohibited. If you have received this communication in error, please > notify us immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information is > strictly prohibited. If you have received this communication in error, please > notify us immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 18 > Date: Wed, 14 Feb 2007 10:22:24 -0600 > From: "Janice Mahoney" > Subject: Re: [Histonet] surgical brain specimens > To: "Paula F. Conlon" , > > Message-ID: <45D2E2E00200003C0000525D@gwia.alegent.org> > Content-Type: text/plain; charset=US-ASCII > > The first and probably most obvious would be that if you are using an > adhesive in your waterbath you should not use it with plus slides. > The second would be to let your sections dry at room temp for a while > before putting gin the oven. > Hope this gives you a place to start. > Jan > Omaha > > >>>> "Conlon, Paula F." 02/14/2007 10:13 AM >>>> > Hi Histonetters, We are receiving more surgical brain sections than we > have in the past. We process the specimens on the Pathos Automatic > Microwave Histoprocessor and we cut the sections at 5 microns. The > sections sometimes do not stay on the slides even though we use Plus > slides. The Neuropathologist is not happy and she has asked me to find > out if there is some way to get better sections. Thank you in advance > for your help. > > Paula Conlon > > Histology Supervisor > > Lahey Clinic > > 41 Mall Road > > Burlington, MA > > 01805 > > 781-744-1345 > > > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL > AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the > intended recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 19 > Date: Wed, 14 Feb 2007 10:25:35 -0600 > From: "Janice Mahoney" > Subject: RE: [Histonet] Special Stainers > To: "RENEE FISHER" , > , "Joyce Weems" > Message-ID: <45D2E39F0200003C00005267@gwia.alegent.org> > Content-Type: text/plain; charset=US-ASCII > > We have tried several and my techs keep preferring Ventana's Nexis. It > is so easy to set up and really is walk away. The only down side is > that it is not continuous feed but the runs are relatively short and we > can do many runs a day if needed. > Jan > Omaha > >>>> "Weems, Joyce" 02/14/2007 10:10 AM >>> > Me too!!! > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of patsy > ruegg > Sent: Wednesday, February 14, 2007 10:57 AM > To: 'RENEE FISHER'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Special Stainers > > > I would vote for the Dako Artisan as well. > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE > FISHER > Sent: Wednesday, February 14, 2007 6:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Stainers > > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ____________________________________________________________________________ > ___________ > > This email may contain confidential protected health information > and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you > are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly > prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. Thank you. > Saint Joseph's Health System, Inc. > > > > > ------------------------------ > > Message: 20 > Date: Wed, 14 Feb 2007 10:31:02 -0600 > From: "Janice Mahoney" > Subject: Re: [Histonet] surgical brain specimens > To: "Janice Mahoney" , "Paula F. Conlon" > , > Message-ID: <45D2E4E60200003C00005284@gwia.alegent.org> > Content-Type: text/plain; charset=US-ASCII > > OOOPS for me too. I don't like my gin in the oven, I prefer it with > tonic over ice. > >>>> "Janice Mahoney" 02/14/2007 10:22 AM >>> > The first and probably most obvious would be that if you are using an > adhesive in your waterbath you should not use it with plus slides. > The second would be to let your sections dry at room temp for a while > before putting gin the oven. > Hope this gives you a place to start. > Jan > Omaha > > >>>> "Conlon, Paula F." 02/14/2007 10:13 AM >>>> > Hi Histonetters, We are receiving more surgical brain sections than we > have in the past. We process the specimens on the Pathos Automatic > Microwave Histoprocessor and we cut the sections at 5 microns. The > sections sometimes do not stay on the slides even though we use Plus > slides. The Neuropathologist is not happy and she has asked me to find > out if there is some way to get better sections. Thank you in advance > for your help. > > Paula Conlon > > Histology Supervisor > > Lahey Clinic > > 41 Mall Road > > Burlington, MA > > 01805 > > 781-744-1345 > > > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL > AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the > intended recipient, your use of this message for any purpose is > strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 21 > Date: Wed, 14 Feb 2007 08:33:34 -0800 > From: "Weimin Qi" > Subject: [Histonet] Astrocytoma slides > To: > Message-ID: <20070214163254.ONYK9790.imta01a2.registeredsite.com@Wqi> > Content-Type: text/plain; charset="us-ascii" > > Does any one know a good source to get astrocytoma slides? Thank you in > advance. > > Weimin > > > > > > ------------------------------ > > Message: 22 > Date: Wed, 14 Feb 2007 11:40:57 -0500 > From: "Amy Porter" > Subject: Re: [Histonet] surgical brain specimens > To: "Conlon, Paula F." , > > Message-ID: <002401c75056$e705e040$8e7a0923@histolab> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Paula - it would definately slow your TAT - here at MSU we used to house the > Mid Michigan Dementia Network. We sectioned all of our well fixede > routinely processed brains at the appropriate thickness for the stain to be > applied and ALWAYS let them air dry completely overnight before placing in > drying oven. This usually gave us about a 95% success rate for section > retention. Hope this helps out. Amy > > Amy S. Porter, HT (ASCP) QIHC > Investigative HistoPathology Laboratory - Supervisor > 2201 Biomedical Physical Sciences Bldg. Rm #2133 > East Lansing, MI 48824-3320 > Phone: (517) 355-6475 ext 1480 > Fax: (517) 432-1368 > Email: portera@msu.edu > Web: www.humanpathology.msu.edu > ----- Original Message ----- > From: "Conlon, Paula F." > To: > Sent: Wednesday, February 14, 2007 11:13 AM > Subject: [Histonet] surgical brain specimens > > > Hi Histonetters, We are receiving more surgical brain sections than we > have in the past. We process the specimens on the Pathos Automatic > Microwave Histoprocessor and we cut the sections at 5 microns. The > sections sometimes do not stay on the slides even though we use Plus > slides. The Neuropathologist is not happy and she has asked me to find > out if there is some way to get better sections. Thank you in advance > for your help. > > Paula Conlon > > Histology Supervisor > > Lahey Clinic > > 41 Mall Road > > Burlington, MA > > 01805 > > 781-744-1345 > > > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 23 > Date: Wed, 14 Feb 2007 10:38:45 -0600 > From: "Dawson, Glen" > Subject: RE: [Histonet] Special Stainers > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Renee, > > The Artisan sold by Dako produces great results. > > Glen Dawson > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of RENEE > FISHER > Sent: Wednesday, February 14, 2007 7:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Stainers > > > Greetings All- > > We are considering a reagent rental on an automatic special stainer. > Does anyone have a good experience with a particular instrument and if > so can we get the contact information? > > Thanks > > ______________________________________________________________________________ > _________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally > privileged under local, state, or federal law. This information is > intended only for the use of the individual or individuals who have > received this. The authorized recipient of this information is > prohibited from disclosing this information to any other party unless > required to do so by law. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. > If you have received this email in error, please notify the sender > immediately to arrange for the disposal of this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 39, Issue 23 > **************************************** Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From HoustonR <@t> chi.osu.edu Wed Feb 14 11:46:26 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Wed Feb 14 11:46:57 2007 Subject: [Histonet] Special Stainers Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77CF81@chi2k3ms01.columbuschildrens.net> The only special stainer to consider, in my opinion, is the Artisan from Dako. Quality of preparations is far superior to anything else on the market. Ronnie Houston MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Wednesday, February 14, 2007 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers Greetings All- We are considering a reagent rental on an automatic special stainer. Does anyone have a good experience with a particular instrument and if so can we get the contact information? Thanks ________________________________________________________________________ _______________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. 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From asmith <@t> mail.barry.edu Wed Feb 14 12:17:09 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Feb 14 12:17:21 2007 Subject: [Histonet] surgical brain specimens In-Reply-To: <348626.51157.qm@web61223.mail.yahoo.com> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4047@exchsrv01.barrynet.barry.edu> I have found that Vector Labs "Vectabond" holds sections on through almost any abuse. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 14, 2007 11:58 AM To: Conlon, Paula F.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surgical brain specimens Paula: You ask about better sections or better retention? We used to section brain at more than 5 ?m, specially if there was the idea of silver staining for Alzheimer's; 5 ?m are too thin to visualize the tangles. For retention we always stained the slides the next day they were cut. The slides were placed vertical on the smaller side for about 1 hour before being put in the trays, that were left outside the oven during the night. The next day they were placed in the oven at 60?C for 30 minutes before being stained (either routine ot HC). Ren? J. "Conlon, Paula F." wrote: Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! 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Barry University - Miami Shores, FL (http://www.barry.edu) From gagnone <@t> KGH.KARI.NET Wed Feb 14 14:23:21 2007 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Feb 14 14:23:24 2007 Subject: [Histonet] p16 Pap Smears on Ventana Benchmark XT Message-ID: Hi Fellow Histonetters, We are currently attempting p16 immunostaining on Pap smears which have been spray-fixed with a 95% ethanol spray fixative then run on the Ventana Benchmark XT. I should add that the slides are post-fixed in formalin, and run with cell conditioning (antigen retrieval). This protocol has worked well for most slides so far. There are some cases in which there are some positive cells staining, but it appears that other cells in other locations on the slide show cells that should likely stain, but aren't. Eventually, ThinPreps will be used in place of Pap smears, and would provide a more uniform preparation. For now, we are going to be attempting to add more antisera to each slide, to try to eliminate possible non-spreading of antisera on the slide. Our other markers, including p16 on paraffin sections are working fine, so we have ruled out mechanical/instrument-related factors. Has anyone else experienced problems with (notoriously irregular) Pap smears for p16, and what worked for you? Thanks for your assistance, Eric Gagnon, MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From JWilkinson <@t> adclinic.com Wed Feb 14 14:26:20 2007 From: JWilkinson <@t> adclinic.com (Wilkinson, Joyce E) Date: Wed Feb 14 14:26:51 2007 Subject: [Histonet] xylene substitute Message-ID: Hello Everyone, I know that this has been asked before. But, what is the best xylene substitute for surgical specimens. Thank you Joyce From froyer <@t> bitstream.net Wed Feb 14 14:26:51 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Feb 14 14:27:02 2007 Subject: [Histonet] Texas Histo Meeting In-Reply-To: References: Message-ID: <001601c75076$75cab390$7701a80a@Ford> Would the Exhibits Chairperson for the Texas Society of Histotechnology please contact me? Thank you, ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com From DELONG_CYNTHIA_A <@t> LILLY.COM Wed Feb 14 14:37:43 2007 From: DELONG_CYNTHIA_A <@t> LILLY.COM (Cynthia A Delong) Date: Wed Feb 14 14:40:14 2007 Subject: [Histonet] GFP antibody Message-ID: Hello all, I am looking for a good working protocol for GFP antibody with possibly a photo. I am working a little blind because of no control. I am under the understanding that when you fix the GFP mouse that there is a loss of staining. My question is can you pull it back out with a GFP antibody since the sites should still be there. I have non fixed frozen brain sections cut at 10 microns. Any advice would be appreciated. Thank you, Cindy From jlinda <@t> ces.clemson.edu Wed Feb 14 14:39:24 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Wed Feb 14 14:40:18 2007 Subject: [Histonet] RE: Hard Tissue Network Message-ID: <5.2.1.1.2.20070214152918.01faf170@mailhost.ces.clemson.edu> Hi, Derek(soon-to-be another "bonehead)! Go to: http://www.e-guana.net/organizations.php3?action=printContentItem&orgid=111&typeID=1162&itemID=17789 for the Hard Tissue website. Go to: http://www.e-guana.net/organizations.php3?action=printContentItem&orgid=111&typeID=1162&itemID=17800 for VIR. These are both very useful committees, especially if you are just getting started with plastic. Also, the Journal of Histotechnology has a wealth of information on plastics. Best wishes and here's hoping your plastic sections cure without bubbles...LOL Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From jbrod <@t> tvmdl.tamu.edu Wed Feb 14 15:03:28 2007 From: jbrod <@t> tvmdl.tamu.edu (Jordan Brod) Date: Wed Feb 14 15:04:17 2007 Subject: [Histonet] Shandon Pathcentre Baskets Message-ID: <45D324C00200005300000276@CS_OFFICE.tamu.edu> Good Afternoon All: I would like to replace the stainless baskets for a Shandon Pathcentre tissue processor. Does anyone have any baskets that they are not using and would like to get rid of? I also am in the market for the "holder" of the baskets and the clips. Please reply to my message if you can help. Thanks. Jordan TVMDL-College Station, TX From ander093 <@t> tc.umn.edu Wed Feb 14 15:38:14 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Feb 14 15:42:28 2007 Subject: [Histonet] surgical brain specimens In-Reply-To: <348626.51157.qm@web61223.mail.yahoo.com> References: <348626.51157.qm@web61223.mail.yahoo.com> Message-ID: <6.2.3.4.0.20070214153342.03ba65e0@ander093.email.umn.edu> Hi Rene and Paula, I have been using 5 micron sections of brain for Bielschowski Silver stain for the last 15 years. The tangles show up just fine. I do let my special's slides air dry overnight though. I also use a small amount of gelatin in my water bath and use regular, non charged slides. If I cut H&E's and need to stain the same day, I let them dry well at RT or in a 40 degree drying oven before putting in 60 degree oven and staining. I rarely (can't remember the last time) lose tissue. LuAnn Anderson HT(ASCP) Neuropathology Lab University of MInnesota At 10:57 AM 2/14/2007, Rene J Buesa wrote: >Paula: > You ask about better sections or better retention? > We used to section brain at more than 5 ?m, > specially if there was the idea of silver > staining for Alzheimer's; 5 ?m are too thin to visualize the tangles. > For retention we always stained the slides > the next day they were cut. The slides were > placed vertical on the smaller side for about 1 > hour before being put in the trays, that were > left outside the oven during the night. The > next day they were placed in the oven at 60?C > for 30 minutes before being stained (either routine ot HC). > Ren? J. > >"Conlon, Paula F." wrote: > Hi Histonetters, We are receiving more surgical brain sections than we >have in the past. We process the specimens on the Pathos Automatic >Microwave Histoprocessor and we cut the sections at 5 microns. The >sections sometimes do not stay on the slides even though we use Plus >slides. The Neuropathologist is not happy and she has asked me to find >out if there is some way to get better sections. Thank you in advance >for your help. > >Paula Conlon > >Histology Supervisor > >Lahey Clinic > >41 Mall Road > >Burlington, MA > >01805 > >781-744-1345 > > > > >See our web page at http://www.lahey.org for a >full directory of Lahey sites, staff, services and career opportunities. > >THIS MESSAGE IS INTENDED FOR THE USE OF THE >PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN >INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND >EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If >you are not the intended recipient, your use of >this message for any purpose is strictly >prohibited. If you have received this >communication in error, please delete the >message and notify the sender so that we may correct our records. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >The fish are biting. > Get more visitors on your site using Yahoo! Search Marketing. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DELONG_CYNTHIA_A <@t> LILLY.COM Wed Feb 14 16:03:57 2007 From: DELONG_CYNTHIA_A <@t> LILLY.COM (Cynthia A Delong) Date: Wed Feb 14 16:02:32 2007 Subject: [Histonet] GFP - found information in archive Message-ID: Sorry all, I believe I found the information I needed in the wonderful Histonet archive. That is what I get for being in a hurry when a post doc says Do you know anything about and can you.......... I thougth the histonet would be the fastest way and forgetting about the archive. Sorry for bothering anyone but if anyone has a NEW and improved way and wish to share feel free to send anything my way I am always looking for the better and faster way of doing things. One question what is the best thickness to view GFP? Thanks again Cindy Cynthia A DeLong LRL - Corporate Center DC0533 - 48B/3042 355 E Merrill St Indianapolis, IN 46225 phone: 317-276-7635 fax: 317-277-6146 From plaurie <@t> benaroyaresearch.org Wed Feb 14 17:24:53 2007 From: plaurie <@t> benaroyaresearch.org (Patrick Laurie) Date: Wed Feb 14 17:28:58 2007 Subject: [Histonet] Reason for lengthy primary antibody incubation? Message-ID: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Hello histonet, I've been a clinical histotech for about 6 years, and I made a transition into research histology about 2 years ago. I hadn't done any IHC at my research postion until recently, and I have noticed a couple of strange differences. First, there are a wide variety of IHC protocols different methods, etc. and I understand the reason for that. But some of them have dramatic differences. One collaborating lab has an incubation of the primary antibody (Ubiquitin) overnight (16-20 hrs) at 4 degrees in a "Hydrated chamber". The primary is quite dilute (1:40,000), and another lab adds a similar step for the secondary antibody. In my clinical experience, I had primarily used a kit, either the Dako Envision or LSAB kit, or the Vector elite kit. My PI, who has no IHC experience until now, had me try the protocol with an overnight incubation, and now since it worked, he wants me to do every antibody from now on like that. He was told by various post docs, etc who have been doing research histology that doing a diluted overnight antibody will make the primary stick better. I know that research has a lot of un-optimized antibodies that might be created in-house or might be for another purpose, so a lengthy primary might make sense. But is there any reason to do this method for clinically tried and true antibodies? I would appreciate all opinions. Thanks, Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 From pruegg <@t> ihctech.net Wed Feb 14 18:09:46 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Wed Feb 14 18:09:56 2007 Subject: [Histonet] Reason for lengthy primary antibody incubation? In-Reply-To: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Message-ID: <200702150009.l1F09igV025076@pro12.abac.com> Your PI will probably want to see for their if it makes a difference, if you have the change I would run side by side test of overnight incubation with very dilute antibody and the usual more speedy method to show him. You might learn something yourself that way as well or not. Using very dilute antibodies will definitely save $ and can cut down on non specific background problems. One of the first things I do to optimize a new antibody is overnight incubation of the primary antibody at least. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Wednesday, February 14, 2007 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reason for lengthy primary antibody incubation? Hello histonet, I've been a clinical histotech for about 6 years, and I made a transition into research histology about 2 years ago. I hadn't done any IHC at my research postion until recently, and I have noticed a couple of strange differences. First, there are a wide variety of IHC protocols different methods, etc. and I understand the reason for that. But some of them have dramatic differences. One collaborating lab has an incubation of the primary antibody (Ubiquitin) overnight (16-20 hrs) at 4 degrees in a "Hydrated chamber". The primary is quite dilute (1:40,000), and another lab adds a similar step for the secondary antibody. In my clinical experience, I had primarily used a kit, either the Dako Envision or LSAB kit, or the Vector elite kit. My PI, who has no IHC experience until now, had me try the protocol with an overnight incubation, and now since it worked, he wants me to do every antibody from now on like that. He was told by various post docs, etc who have been doing research histology that doing a diluted overnight antibody will make the primary stick better. I know that research has a lot of un-optimized antibodies that might be created in-house or might be for another purpose, so a lengthy primary might make sense. But is there any reason to do this method for clinically tried and true antibodies? I would appreciate all opinions. Thanks, Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From coleman_manufacturing <@t> yahoo.com Wed Feb 14 19:08:26 2007 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Wed Feb 14 19:12:12 2007 Subject: [Histonet] Microwave Processing accessories.....thanks ! Message-ID: <808623.90644.qm@web37611.mail.mud.yahoo.com> To all customers: From everyone here at Coleman Manufacturing & Design we would like to thank all who order plastic microwave accessories from our company. Coleman gained an upper hand in supplying accessories against OEM manufacturers in 2006; we expect 2007 to be even better. Which means, Coleman will save customers 45% just on plastic accessories. Only six months into 2006 with our accessory sales, we saved customers a combined total of $203,567.36. The savings are significant. In our eyes, if a laboratory or hospital can order accessories from Coleman verses the OEM of the Tissue Processor and save on an average 45% per order with maintaining the same durability and longevity, why not? Again, thanks to all who order here at Coleman. For those of you who do not know about us please call and request a catalog. We supply plastic accessories that accommodate ALL TISSUE PROCESSING MICROWAVES; EVEN GLASS BEAKERS FOR VACUUM AND NON-VACUUM. Best regards, CMD From barb94117 <@t> msn.com Wed Feb 14 19:37:08 2007 From: barb94117 <@t> msn.com (barbara albert) Date: Wed Feb 14 19:38:42 2007 Subject: [Histonet] position in San Francisco Message-ID: We have openings for routine bench histotechs here at UCSF. search for job # 21074BR Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco From barb94117 <@t> msn.com Wed Feb 14 19:37:08 2007 From: barb94117 <@t> msn.com (barbara albert) Date: Wed Feb 14 19:39:09 2007 Subject: [Histonet] position in San Francisco Message-ID: We have openings for routine bench histotechs here at UCSF. search for job # 21074BR Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco From ree3 <@t> leicester.ac.uk Thu Feb 15 03:24:42 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Feb 15 03:25:18 2007 Subject: [Histonet] Reason for lengthy primary antibody incubation? In-Reply-To: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> References: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Message-ID: In immunohistochemistry the following truism is applicable "What works for you works for you; what works for me, works for me" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: 14 February 2007 23:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reason for lengthy primary antibody incubation? Hello histonet, I've been a clinical histotech for about 6 years, and I made a transition into research histology about 2 years ago. I hadn't done any IHC at my research postion until recently, and I have noticed a couple of strange differences. First, there are a wide variety of IHC protocols different methods, etc. and I understand the reason for that. But some of them have dramatic differences. One collaborating lab has an incubation of the primary antibody (Ubiquitin) overnight (16-20 hrs) at 4 degrees in a "Hydrated chamber". The primary is quite dilute (1:40,000), and another lab adds a similar step for the secondary antibody. In my clinical experience, I had primarily used a kit, either the Dako Envision or LSAB kit, or the Vector elite kit. My PI, who has no IHC experience until now, had me try the protocol with an overnight incubation, and now since it worked, he wants me to do every antibody from now on like that. He was told by various post docs, etc who have been doing research histology that doing a diluted overnight antibody will make the primary stick better. I know that research has a lot of un-optimized antibodies that might be created in-house or might be for another purpose, so a lengthy primary might make sense. But is there any reason to do this method for clinically tried and true antibodies? I would appreciate all opinions. Thanks, Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Feb 15 06:53:39 2007 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Thu Feb 15 06:57:05 2007 Subject: [Histonet] Reason for lengthy primary antibody incubation? In-Reply-To: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> References: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Message-ID: <1171544019.45d457d34ced2@imp.vet.upenn.edu> Hi Patrick, I agree with Patsy and would add we often extend a very expensive anitbody to overnight. They do to allow very extended dilutions out to the 1:40,000 is an extreme example and allows more slides to reacted. Research generally has more time to do these longer times and the mind set. We are in the position in research of needing to have a patient's slides completed on the same time day. I have some antibodies we use on the short clinical type time frame and some we extend to allow the use of less anitbody in the dilutions. I do use some of the secondary kits however as we do a large range of animals and no mice or rats we have to be very careful which animals are used in these kits to avoid heavy background. Best instance is using the Vector kit when we are doing equine material as it is a horse serum block and some cross reactivity of the second antibody. Research is a very different mindset from clinical and although we have time pressures at times just like clinical. However, we also the time to workout protocols and make use of some methods not used in clinical due the time issues. Pamela Marcun UPENN New Bolton Center quoting Patrick Laurie : > Hello histonet, > > > > I've been a clinical histotech for about 6 years, and I made a > transition into research histology about 2 years ago. I hadn't done any > IHC at my research postion until recently, and I have noticed a couple > of strange differences. First, there are a wide variety of IHC > protocols different methods, etc. and I understand the reason for that. > But some of them have dramatic differences. One collaborating lab has > an incubation of the primary antibody (Ubiquitin) overnight (16-20 hrs) > at 4 degrees in a "Hydrated chamber". The primary is quite dilute > (1:40,000), and another lab adds a similar step for the secondary > antibody. In my clinical experience, I had primarily used a kit, either > the Dako Envision or LSAB kit, or the Vector elite kit. My PI, who has > no IHC experience until now, had me try the protocol with an overnight > incubation, and now since it worked, he wants me to do every antibody > from now on like that. He was told by various post docs, etc who have > been doing research histology that doing a diluted overnight antibody > will make the primary stick better. I know that research has a lot of > un-optimized antibodies that might be created in-house or might be for > another purpose, so a lengthy primary might make sense. But is there > any reason to do this method for clinically tried and true antibodies? > I would appreciate all opinions. > > > > Thanks, > > > > Patrick Laurie, HT (ASCP) > Neurogenomics Laboratory > Benaroya Research Institute > 1201 9th Ave > Seattle, Wa 98101 > (206) 341-0681 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anh2006 <@t> med.cornell.edu Thu Feb 15 08:06:42 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Thu Feb 15 08:10:57 2007 Subject: [Histonet] Reason for lengthy primary antibody incubation? In-Reply-To: <1171544019.45d457d34ced2@imp.vet.upenn.edu> References: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> <1171544019.45d457d34ced2@imp.vet.upenn.edu> Message-ID: 1:40,000 means nothing without knowing the stock concentration of the antibody. If the antibody was 10mg/ml (as it sometimes the case especially with antibodies made in house) using it at 1:40,000 would not be so unbelievable as a stock of 500ug/ml being used at 1:40,000. In research the people doing IHC are often the same ones doing every other type of experiment in the lab that day. What I am saying that often times the slides are put up overnight simply b/c time runs out. I sometimes do my blocking step overnight and sometimes antibody. To me, I have rarely seen overnight make any major "night-and-day" sort of difference. For example, if my staining didn't work at all with a 1-2 hr at RT incubation it wasn't going to magically appear all beautiful after an overnight incubation. At 7:53 AM -0500 2/15/07, pmarcum@vet.upenn.edu wrote: >quoting Patrick Laurie : > > > Hello histonet, > > > > > > > > I've been a clinical histotech for about 6 years, and I made a > > transition into research histology about 2 years ago. I hadn't done any > > IHC at my research postion until recently, and I have noticed a couple > > of strange differences. First, there are a wide variety of IHC > > protocols different methods, etc. and I understand the reason for that. > > But some of them have dramatic differences. One collaborating lab has >> an incubation of the primary antibody (Ubiquitin) overnight (16-20 hrs) >> at 4 degrees in a "Hydrated chamber". The primary is quite dilute >> (1:40,000), and another lab adds a similar step for the secondary >> antibody. In my clinical experience, I had primarily used a kit, either >> the Dako Envision or LSAB kit, or the Vector elite kit. My PI, who has >> no IHC experience until now, had me try the protocol with an overnight >> incubation, and now since it worked, he wants me to do every antibody >> from now on like that. He was told by various post docs, etc who have >> been doing research histology that doing a diluted overnight antibody >> will make the primary stick better. I know that research has a lot of >> un-optimized antibodies that might be created in-house or might be for >> another purpose, so a lengthy primary might make sense. But is there >> any reason to do this method for clinically tried and true antibodies? >> I would appreciate all opinions. >> >> >> >> Thanks, >> >> >> >> Patrick Laurie, HT (ASCP) >> Neurogenomics Laboratory >> Benaroya Research Institute >> 1201 9th Ave >> Seattle, Wa 98101 > > (206) 341-0681 -- From akemiat3377 <@t> yahoo.com Thu Feb 15 08:30:34 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Feb 15 08:37:23 2007 Subject: [Histonet] looking for histology management position on the west coast Message-ID: <467424.6773.qm@web31301.mail.mud.yahoo.com> Hello Histonetters--I know of a histology manager that is looking for a position on the west coast. The candidate has extensive management expierience in Biotech histology, IHC & TMA. If any of you know of an availability, please contact me via e-mail or phone. Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com From hfedor <@t> jhmi.edu Thu Feb 15 08:45:49 2007 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Feb 15 08:50:09 2007 Subject: [Histonet] Tissue Microarray tools In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C012B5AB8@mercury.pa-ucl.com> References: <9FC023A4AB52BB4D87DC6456081A822C012B5AB8@mercury.pa-ucl.com> Message-ID: <45D42BB0.61A1.0088.3@jhmi.edu> Dear Annette, We have been using the Beecher system and have made over 450 TMA's with the instrument, It is reliable and durable. Best Regards, Helen Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. >>> Annette Hall 2/13/2007 2:32 PM >>> Can anyone recommend a good system for making tissue microarrays. We are not a large volume lab (approx 250 IHC per month), so I would be looking at manual methods for accomplishing this. My planned uses would be for new antibody evaluations and controls. Thanks, Annette Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From apothad <@t> gmail.com Thu Feb 15 09:00:05 2007 From: apothad <@t> gmail.com (Adam Kirk) Date: Thu Feb 15 09:00:17 2007 Subject: [Histonet] Aquaporin 2 Message-ID: <40e76ce80702150700m64574729kc3f8a714877dcc44@mail.gmail.com> Hi there Histonetters, I am working human tissue and trying to use the Abcam aquaporin 2 ab (15081). To date I have had absolutely no staining in glycolmethacrylate-embedded tissue. Does anyone have any experience with this specific ab, or are you using the millipore/chemicon one? and if you have success, what preperation have you used for the tissue? I'm starting to lose my hair!!! Thanks Adam Kirk -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 From katherine-walters <@t> uiowa.edu Thu Feb 15 09:58:22 2007 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Thu Feb 15 09:58:36 2007 Subject: [Histonet] Aquaporin 2 In-Reply-To: <40e76ce80702150700m64574729kc3f8a714877dcc44@mail.gmail.com> Message-ID: Hi Adam, I have done quite a bit of Aquaporin labeling (1,2,3,&4). I tried Alpha Diagnostic (AQP23-A), Chemicon (AB3274-50ul) and Santa Cruz (sc-9880). I had no luck with Alpha Diagnostics or Santa Cruz, but very good labeling with the Chemicon antibody. I use it at 1:500 with heat retrieval on paraffin sections. I hope this will save what's left of your hair, I lost a little myself with this one!! Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam Kirk Sent: Thursday, February 15, 2007 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aquaporin 2 Hi there Histonetters, I am working human tissue and trying to use the Abcam aquaporin 2 ab (15081). To date I have had absolutely no staining in glycolmethacrylate-embedded tissue. Does anyone have any experience with this specific ab, or are you using the millipore/chemicon one? and if you have success, what preperation have you used for the tissue? I'm starting to lose my hair!!! Thanks Adam Kirk -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Feb 15 09:57:30 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Feb 15 10:01:33 2007 Subject: [Histonet] Tissue Microarray tools In-Reply-To: <45D42BB0.61A1.0088.3@jhmi.edu> Message-ID: <382680.55290.qm@web31313.mail.mud.yahoo.com> Helen, I just saw a new "TMA Builder" that Lab Vision has available cat# TMA-001 under Research Tools in their new catalog. It looks similar to what woorie-medic's tissue punch extractor looked like. The mold is totally different though, it has a metal clamp & a metal recipient base mold with 24 core indentations. Have you seen it? Any comments? Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com Helen Fedor wrote: Dear Annette, We have been using the Beecher system and have made over 450 TMA's with the instrument, It is reliable and durable. Best Regards, Helen Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. >>> Annette Hall 2/13/2007 2:32 PM >>> Can anyone recommend a good system for making tissue microarrays. We are not a large volume lab (approx 250 IHC per month), so I would be looking at manual methods for accomplishing this. My planned uses would be for new antibody evaluations and controls. Thanks, Annette Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 15 10:48:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 15 10:48:55 2007 Subject: [Histonet] Reason for lengthy primary antibody incubation? In-Reply-To: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Message-ID: <354764.48293.qm@web61222.mail.yahoo.com> Laurie: Incubation time sometimes depend on the setting you are doing the IHC. Research setting usually has no big time constrains, but a clinical setting does. In the clinical setting overnight incubation is simply a nightmare because of the required (and patient deserved) short TAT. On the other hand for both manual and automated IHC protocols, ALL incubation times should be the same for ALL antibodies, otherwise it will be just be very difficult, and prone to mistakes, having different times for each Ab. He (or she) who told you that overnight incubation makes Ab to "stick better" does not know what is talking about. This is not a "sticking" issue; Ab will react to the antigenic sites and once they do, they do, regardless. On the other hand incubating at 4?C means that the reaction will take at least twice slower than at room temperature (van't Hoff, remember?). Great dilution will cut costs and may reduce background noise for the reaction, but is not a great advantage. Finally a piece of advise: you could tell you reseracher what I used to say to those who wanted to dictate me how to work: I always told them, "tell me what you want me to do, but do not tell me how to do it!" Hope this will help you! Ren? J. Patrick Laurie wrote: Hello histonet, I've been a clinical histotech for about 6 years, and I made a transition into research histology about 2 years ago. I hadn't done any IHC at my research postion until recently, and I have noticed a couple of strange differences. First, there are a wide variety of IHC protocols different methods, etc. and I understand the reason for that. But some of them have dramatic differences. One collaborating lab has an incubation of the primary antibody (Ubiquitin) overnight (16-20 hrs) at 4 degrees in a "Hydrated chamber". The primary is quite dilute (1:40,000), and another lab adds a similar step for the secondary antibody. In my clinical experience, I had primarily used a kit, either the Dako Envision or LSAB kit, or the Vector elite kit. My PI, who has no IHC experience until now, had me try the protocol with an overnight incubation, and now since it worked, he wants me to do every antibody from now on like that. He was told by various post docs, etc who have been doing research histology that doing a diluted overnight antibody will make the primary stick better. I know that research has a lot of un-optimized antibodies that might be created in-house or might be for another purpose, so a lengthy primary might make sense. But is there any reason to do this method for clinically tried and true antibodies? I would appreciate all opinions. Thanks, Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. From lpaveli1 <@t> hurleymc.com Thu Feb 15 11:03:01 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Feb 15 11:03:42 2007 Subject: [Histonet] xylene substitute Message-ID: <45D44BF5020000EE00011643@smtp-gw.hurleymc.com> We recently changed from xylene to a substitute called Propar from Anatech. Easy transition, and actually have found specimens to not be as "dry" as when we used xylene. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Wilkinson, Joyce E" 02/14/07 3:26 PM >>> Hello Everyone, I know that this has been asked before. But, what is the best xylene substitute for surgical specimens. Thank you Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Thu Feb 15 11:31:17 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Feb 15 11:31:28 2007 Subject: [Histonet] Thermometers Message-ID: <5DA1CA5D0B98A84985B545A24423B822052D11@UPLAB01.uplab.local> Do labs generally recalibrate/recertify NIST calibrated thermometers? If so, how and how often? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From hymclab <@t> hyhc.com Thu Feb 15 11:39:52 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Thu Feb 15 11:33:38 2007 Subject: [Histonet] xylene substitute Message-ID: We have used the Thermo brand (used to be called Histosolve, now just Xylene Substitute) for the last 16 years. We love it!! Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Lynette Pavelich [mailto:lpaveli1@hurleymc.com] Sent: Thursday, February 15, 2007 11:03 AM To: JWilkinson@adclinic.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] xylene substitute We recently changed from xylene to a substitute called Propar from Anatech. Easy transition, and actually have found specimens to not be as "dry" as when we used xylene. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Wilkinson, Joyce E" 02/14/07 3:26 PM >>> Hello Everyone, I know that this has been asked before. But, what is the best xylene substitute for surgical specimens. Thank you Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From liz <@t> premierlab.com Thu Feb 15 12:16:54 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 15 12:07:47 2007 Subject: [Histonet] Thermometers In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822052D11@UPLAB01.uplab.local> Message-ID: <000001c7512d$7af456c0$0d00a8c0@domain.Premier> It depends upon your SOP's most calibrated thermometers have a little statement at the bottom of the certification that states that most institutions recalibrate once a year but you must follows your own SOP's. You then need to take into consideration what guidelines you are following and what the standards are. What I'm saying is that you can write in your SOP's that you are going to calibrate once every 5 years, but when you get inspected will the inspector think that is good enough. The kicker is the cost to recalibrate is more than a new thermometer, we just purchase a new calibrated thermometer each year and that's what is in our SOP's. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff Sent: Thursday, February 15, 2007 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermometers Do labs generally recalibrate/recertify NIST calibrated thermometers? If so, how and how often? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From steven.p.postl <@t> abbott.com Thu Feb 15 12:58:43 2007 From: steven.p.postl <@t> abbott.com (Steven P Postl) Date: Thu Feb 15 12:59:20 2007 Subject: [Histonet] Looking for Contact Message-ID: If Mary Gessford is out there, could you please contact me? Thanks. From making <@t> ufl.edu Thu Feb 15 13:17:16 2007 From: making <@t> ufl.edu (MKing) Date: Thu Feb 15 13:12:04 2007 Subject: [Histonet] primary incubation times Message-ID: <45D4B1BC.2050305@ufl.edu> Patrick, I remember reading in one of the early immunohistochemistry texts that the reaction between a primary antibody and its epitope was believed to require 96 hrs. at 4 deg. C to reach equilibration. Whether or not that's true for your antibodies, it is the kind of precedent that underlies the procedures typically used today. The other issue is penetration--if you need tissue to be evenly labeled throughout the depth of the specimen then section thickness and embedding material may require long incubations (at least). If surface labeling of an abundant epitope is enough to tell you what you want to know, a good primary may require less than half an hour! Happy histo, Mike King UF Neuroscience ---------------------------------------- Date: Wed, 14 Feb 2007 15:24:53 -0800 From: "Patrick Laurie" Subject: [Histonet] Reason for lengthy primary antibody incubation? To: "histonet@lists.utsouthwestern.edu" Message-ID: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Content-Type: text/plain; charset="us-ascii" From joseph.tamasi <@t> bms.com Thu Feb 15 14:26:10 2007 From: joseph.tamasi <@t> bms.com (Joseph Tamasi) Date: Thu Feb 15 14:26:52 2007 Subject: [Histonet] New Jersey Symposium Message-ID: <45D4C1E2.208@bms.com> Dear Histonet Subscribers that live in or around New Jersey: The New Jersey Society for Histotechnology is offering a two day symposium on March 16-17, 2007 at the Clarion Hotel and Conference Center in Cherry Hill, NJ. You can view the complete program and get registration information on the NSH website. Go to www.NSH.org, then on the left hand side of the page click on NSH Regions / Meeting Calendar / Region II / NJSH Symposium. Scroll down for the link to view the full program. From Annette_hall <@t> pa-ucl.com Thu Feb 15 14:50:13 2007 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Thu Feb 15 14:50:25 2007 Subject: [Histonet] Tissue Microarray tools Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5AD8@mercury.pa-ucl.com> I just received the catalog yesterday and was thrilled to see this product. Although a bit pricey-$1530, we should be able to capture the cost with reagent and materials savings, as well as tech time. Thanks to all of you who responded to my inquiry, Annette -----Original Message----- From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Thursday, February 15, 2007 9:58 AM To: Helen Fedor Cc: histonet Subject: Re: [Histonet] Tissue Microarray tools Helen, I just saw a new "TMA Builder" that Lab Vision has available cat# TMA-001 under Research Tools in their new catalog. It looks similar to what woorie-medic's tissue punch extractor looked like. The mold is totally different though, it has a metal clamp & a metal recipient base mold with 24 core indentations. Have you seen it? Any comments? Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com Helen Fedor wrote: Dear Annette, We have been using the Beecher system and have made over 450 TMA's with the instrument, It is reliable and durable. Best Regards, Helen Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. >>> Annette Hall 2/13/2007 2:32 PM >>> Can anyone recommend a good system for making tissue microarrays. We are not a large volume lab (approx 250 IHC per month), so I would be looking at manual methods for accomplishing this. My planned uses would be for new antibody evaluations and controls. Thanks, Annette Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From JGREWE <@t> OhioHealth.com Thu Feb 15 15:02:50 2007 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Thu Feb 15 15:03:02 2007 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 02/14/2007 and will not return until 02/19/2007. I will respond to your message when I return. Thanks, Jackie From jamie.erickson <@t> abbott.com Thu Feb 15 16:40:24 2007 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu Feb 15 16:40:32 2007 Subject: [Histonet] Freezing human skin biopsies for cytokine ICC Message-ID: Hello histonetters, I hope someone can help me with a question about how to freeze a human skin biopsy. I am in research but am in collaboration with a clinical lab that will be collecting human skin biopsies for me to do cytokine ICC of TNF,IFN-g, among others and possibly cell makers. My question is how do I instruct them in freezing the skin so that I get the best sample for this staining. I am going to embed the sample once it is in my lab for cryosectioning. Also if people know of manufactures of antibodies that sell TNF,IFN-g that work in human tissue that would also be helpful, I have found a few but many different protocols and fixations. Any thoughts how be helpful. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From gentras <@t> vetmed.auburn.edu Thu Feb 15 17:01:45 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Feb 15 17:05:53 2007 Subject: [Histonet] gelatinized slides Message-ID: <45D4E659.1070909@vetmed.auburn.edu> hello, one of our collaborating researchers has me searching for gelatinized slides to be used for Golgi staining for cryosectioning 100 micron mouse spines. It appears that she came up with this idea after reading about a similar protocol on brain. I have searched the histonet archives and the only thing that I can come up with is info on gelatin subbed slides. Thus far I've only found one company that has commercial gelatinized slides. Please pardon my ignorance but is there a significant difference between the two whereas one is preferable for certain protocols for which the other is not? And if any of you are currently using commercial gelatinized slides will you provide me with contact info on your source? Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From ckduffynw <@t> aol.com Fri Feb 16 10:34:17 2007 From: ckduffynw <@t> aol.com (ckduffynw@aol.com) Date: Fri Feb 16 10:34:33 2007 Subject: [Histonet] HT position in Seattle Message-ID: <8C91FFF6FD678A6-764-19B2@WEBMAIL-RC06.sysops.aol.com> One part-time and one full time position needed immediately in Edmonds area. ASCP cert required. Small lab, excellent benefits, competitve salary. Contact Kay at ckduffynw@aol.com. ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From soofias2 <@t> yahoo.com Fri Feb 16 11:00:17 2007 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Fri Feb 16 11:30:57 2007 Subject: [Histonet] Thermometers In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822052D11@UPLAB01.uplab.local> Message-ID: <201359.87917.qm@web39507.mail.mud.yahoo.com> Initially I found to get new thermometer each year was easier and economical, but after some research I came up with a cheaper solution. Now in our lab we recalibrate NIST calibrated thermometer every year. We keep two sets of thermometers for each freezer and fridge and recalibrate and use them alternate year.One which is currently in use is just got calibrated in January,07and will expire in January,08. The other one which is expiring in Feb ,07 we will send for recalibration in December ,07 which will come back by January,08 and we will use recalibrated one for the next year. We send our thermometer.to the following company. . Calibration Services Control Company 4455 Rex Road Friendswood, TX 77546 They charge $15 for each thermometer and $8 for shipping. We get two thermometer done in $50 including shipping from both ways. I found it cheaper that way then buying new thermometer each year. I believe that calibration can be done in house too. I am sure there are very smart people out there in this field who may have better ways to deal with this issue. Soofia Lester Raff wrote: Do labs generally recalibrate/recertify NIST calibrated thermometers? If so, how and how often? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From HDOWNS <@t> PARTNERS.ORG Fri Feb 16 12:08:38 2007 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Fri Feb 16 12:08:57 2007 Subject: [Histonet] Freezing human skin biopsies for cytokine ICC In-Reply-To: <20070216180105.EDAF5539DB0@phsmgmx2.partners.org> Message-ID: <54DC0778AC30D4418FFF52A7FD2008750220B3DF@PHSXMB19.partners.org> We often section human skin punch biopsy, using a sliding microtome and dry ice. We fix our tissue for 12 hours in PLP, followed by a wash in Sorenson's and placed in croprotectant for 24 hours before either cutting it, or freezing @-20. We do a free floating immunostain and cut at 50 microns with no problems. HD -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 16, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 39, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Thermometers (Liz Chlipala) 2. Looking for Contact (Steven P Postl) 3. primary incubation times (MKing) 4. New Jersey Symposium (Joseph Tamasi) 5. RE: Tissue Microarray tools (Annette Hall) 6. Jacquelyn Grewe/Staff/OhioHealth is out of the office . (JGREWE@OhioHealth.com) 7. Freezing human skin biopsies for cytokine ICC (Jamie E Erickson) 8. gelatinized slides (Atoska Gentry) 9. HT position in Seattle (ckduffynw@aol.com) 10. Re: Thermometers (soofia siddiqui) ---------------------------------------------------------------------- Message: 1 Date: Thu, 15 Feb 2007 11:16:54 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Thermometers To: "'Lester Raff'" , Message-ID: <000001c7512d$7af456c0$0d00a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" It depends upon your SOP's most calibrated thermometers have a little statement at the bottom of the certification that states that most institutions recalibrate once a year but you must follows your own SOP's. You then need to take into consideration what guidelines you are following and what the standards are. What I'm saying is that you can write in your SOP's that you are going to calibrate once every 5 years, but when you get inspected will the inspector think that is good enough. The kicker is the cost to recalibrate is more than a new thermometer, we just purchase a new calibrated thermometer each year and that's what is in our SOP's. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff Sent: Thursday, February 15, 2007 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermometers Do labs generally recalibrate/recertify NIST calibrated thermometers? If so, how and how often? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com ------------------------------ Message: 2 Date: Thu, 15 Feb 2007 12:58:43 -0600 From: Steven P Postl Subject: [Histonet] Looking for Contact To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" If Mary Gessford is out there, could you please contact me? Thanks. ------------------------------ Message: 3 Date: Thu, 15 Feb 2007 14:17:16 -0500 From: MKing Subject: [Histonet] primary incubation times To: histonet@lists.utsouthwestern.edu Message-ID: <45D4B1BC.2050305@ufl.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Patrick, I remember reading in one of the early immunohistochemistry texts that the reaction between a primary antibody and its epitope was believed to require 96 hrs. at 4 deg. C to reach equilibration. Whether or not that's true for your antibodies, it is the kind of precedent that underlies the procedures typically used today. The other issue is penetration--if you need tissue to be evenly labeled throughout the depth of the specimen then section thickness and embedding material may require long incubations (at least). If surface labeling of an abundant epitope is enough to tell you what you want to know, a good primary may require less than half an hour! Happy histo, Mike King UF Neuroscience ---------------------------------------- Date: Wed, 14 Feb 2007 15:24:53 -0800 From: "Patrick Laurie" Subject: [Histonet] Reason for lengthy primary antibody incubation? To: "histonet@lists.utsouthwestern.edu" Message-ID: <420f1b520f9ee44994edfc63ad644f47@localhost.localdomain> Content-Type: text/plain; charset="us-ascii" ------------------------------ Message: 4 Date: Thu, 15 Feb 2007 15:26:10 -0500 From: Joseph Tamasi Subject: [Histonet] New Jersey Symposium To: histonet@lists.utsouthwestern.edu Message-ID: <45D4C1E2.208@bms.com> Content-Type: text/plain; charset="iso-8859-1" Dear Histonet Subscribers that live in or around New Jersey: The New Jersey Society for Histotechnology is offering a two day symposium on March 16-17, 2007 at the Clarion Hotel and Conference Center in Cherry Hill, NJ. You can view the complete program and get registration information on the NSH website. Go to www.NSH.org, then on the left hand side of the page click on NSH Regions / Meeting Calendar / Region II / NJSH Symposium. Scroll down for the link to view the full program. ------------------------------ Message: 5 Date: Thu, 15 Feb 2007 14:50:13 -0600 From: Annette Hall Subject: RE: [Histonet] Tissue Microarray tools To: 'Akemi Allison-Tacha' , Helen Fedor Cc: histonet Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5AD8@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" I just received the catalog yesterday and was thrilled to see this product. Although a bit pricey-$1530, we should be able to capture the cost with reagent and materials savings, as well as tech time. Thanks to all of you who responded to my inquiry, Annette -----Original Message----- From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Thursday, February 15, 2007 9:58 AM To: Helen Fedor Cc: histonet Subject: Re: [Histonet] Tissue Microarray tools Helen, I just saw a new "TMA Builder" that Lab Vision has available cat# TMA-001 under Research Tools in their new catalog. It looks similar to what woorie-medic's tissue punch extractor looked like. The mold is totally different though, it has a metal clamp & a metal recipient base mold with 24 core indentations. Have you seen it? Any comments? Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, IHC & TMA Madison, WI Cell: (925)788-0900 E-mail: akemiat3377@yahoo.com Helen Fedor wrote: Dear Annette, We have been using the Beecher system and have made over 450 TMA's with the instrument, It is reliable and durable. Best Regards, Helen Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. >>> Annette Hall 2/13/2007 2:32 PM >>> Can anyone recommend a good system for making tissue microarrays. We are not a large volume lab (approx 250 IHC per month), so I would be looking at manual methods for accomplishing this. My planned uses would be for new antibody evaluations and controls. Thanks, Annette Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 Cell: 563.580.9751 Fax: 563.584.2085 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 6 Date: Thu, 15 Feb 2007 16:02:50 -0500 From: JGREWE@OhioHealth.com Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 02/14/2007 and will not return until 02/19/2007. I will respond to your message when I return. Thanks, Jackie ------------------------------ Message: 7 Date: Thu, 15 Feb 2007 17:40:24 -0500 From: Jamie E Erickson Subject: [Histonet] Freezing human skin biopsies for cytokine ICC To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello histonetters, I hope someone can help me with a question about how to freeze a human skin biopsy. I am in research but am in collaboration with a clinical lab that will be collecting human skin biopsies for me to do cytokine ICC of TNF,IFN-g, among others and possibly cell makers. My question is how do I instruct them in freezing the skin so that I get the best sample for this staining. I am going to embed the sample once it is in my lab for cryosectioning. Also if people know of manufactures of antibodies that sell TNF,IFN-g that work in human tissue that would also be helpful, I have found a few but many different protocols and fixations. Any thoughts how be helpful. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com ------------------------------ Message: 8 Date: Thu, 15 Feb 2007 17:01:45 -0600 From: Atoska Gentry Subject: [Histonet] gelatinized slides To: Histonet Message-ID: <45D4E659.1070909@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, one of our collaborating researchers has me searching for gelatinized slides to be used for Golgi staining for cryosectioning 100 micron mouse spines. It appears that she came up with this idea after reading about a similar protocol on brain. I have searched the histonet archives and the only thing that I can come up with is info on gelatin subbed slides. Thus far I've only found one company that has commercial gelatinized slides. Please pardon my ignorance but is there a significant difference between the two whereas one is preferable for certain protocols for which the other is not? And if any of you are currently using commercial gelatinized slides will you provide me with contact info on your source? Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu ------------------------------ Message: 9 Date: Fri, 16 Feb 2007 11:34:17 -0500 From: ckduffynw@aol.com Subject: [Histonet] HT position in Seattle To: histonet@lists.utsouthwestern.edu Message-ID: <8C91FFF6FD678A6-764-19B2@WEBMAIL-RC06.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" One part-time and one full time position needed immediately in Edmonds area. ASCP cert required. Small lab, excellent benefits, competitve salary. Contact Kay at ckduffynw@aol.com. ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. ------------------------------ Message: 10 Date: Fri, 16 Feb 2007 09:00:17 -0800 (PST) From: soofia siddiqui Subject: Re: [Histonet] Thermometers To: Lester Raff , histonet@lists.utsouthwestern.edu Message-ID: <201359.87917.qm@web39507.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Initially I found to get new thermometer each year was easier and economical, but after some research I came up with a cheaper solution. Now in our lab we recalibrate NIST calibrated thermometer every year. We keep two sets of thermometers for each freezer and fridge and recalibrate and use them alternate year.One which is currently in use is just got calibrated in January,07and will expire in January,08. The other one which is expiring in Feb ,07 we will send for recalibration in December ,07 which will come back by January,08 and we will use recalibrated one for the next year. We send our thermometer.to the following company. . Calibration Services Control Company 4455 Rex Road Friendswood, TX 77546 They charge $15 for each thermometer and $8 for shipping. We get two thermometer done in $50 including shipping from both ways. I found it cheaper that way then buying new thermometer each year. I believe that calibration can be done in house too. I am sure there are very smart people out there in this field who may have better ways to deal with this issue. Soofia Lester Raff wrote: Do labs generally recalibrate/recertify NIST calibrated thermometers? If so, how and how often? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 26 **************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From Allison_Scott <@t> hchd.tmc.edu Fri Feb 16 12:43:25 2007 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Feb 16 12:47:31 2007 Subject: [Histonet] Mortech Grossing Stations Message-ID: <1872B4A455B7974391609AD8034C79FC0670FB@LBEXCH01.hchd.local> Hello to all in histoloand. Is there anyone that is using the Mortech grossing stations. We just received ours on yesterday, and there are problems already. These are not the stations that I requested. We wanted Thermo Shandon's grosslab Senior. This is what we had on capitol equipment, but in the end this is what we got. I have never heard of this company, and don't know the reliability of the equipment. If any one is using them please contact me. Thanks in advance. Allison Scott CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From dpahisto <@t> yahoo.com Fri Feb 16 12:57:14 2007 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Fri Feb 16 13:01:19 2007 Subject: [Histonet] Thermometers & CLIAA Message-ID: <82726.59317.qm@web33409.mail.mud.yahoo.com> I found it interesting that there is a discussion on thermometers going on. We had a CLIAA inspection yesterday. Our inspector told us we weren't "Certified" to read analog thermometers and we must use digital thermometers for our daily temp readings of all equipment including our refridgerator and waterbaths. Cindy DuBois Integrated Pathology Stockton, CA --------------------------------- Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. From TJJ <@t> Stowers-Institute.org Fri Feb 16 13:20:36 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Feb 16 13:21:00 2007 Subject: [Histonet] Re: Reason for lengthy primary antibody Message-ID: >From the Chemicon website (http://www.chemicon.com/resource/ANT101/a1.asp): >>The time taken to reach equilibrium is dependent on the rate of diffusion and the affinity of the antibody for the antigen, and can vary widely. The affinity constant for antibody-antigen binding can span a wide range, extending from below 105 mol-1 to above 1012 mol-1. Affinity constants can be affected by temperature, pH and solvent. Affinity constants can be determined for monoclonal antibodies, but not for polyclonal antibodies, as multiple bondings take place between polyclonal antibodies and their antigens. << Take the time to read the entire text from this website. It's very good information regarding antibodies and antigens. I agree with many that it's an issue of time convenience with research. The more methods and materials you read in publications, the more you realize that overnight at 4 degrees C is the standard. Why? Some because of time management. I suspect mostly it's because it's always been done that way. It is very useful to only have to do an IHC once (if you are so lucky), and if you have a better chance of getting binding overnight in the refrigerator than 30-60 minutes at room temp, then do it. We have had one antibody we could not get to work, until we incubated it 48 hours in the refrigerator and used a polymer detection. Amazingly, 1 hour at RT and 24 hours in the fridge did not produce any staining. So, yes, it might well make a difference. Having said all that, we routinely do many of our antibody procedures using 1 hour at room temp. But we do not dismiss the need for longer, extended incubation times in some cases. Additionally, all of our whole mount staining is always done overnight. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From micro <@t> superlink.net Fri Feb 16 13:43:32 2007 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Fri Feb 16 13:43:43 2007 Subject: [Histonet] Thermometers & CLIAA References: <82726.59317.qm@web33409.mail.mud.yahoo.com> Message-ID: <021901c75202$bdc97fe0$6b893cd1@DJ4VDH31> Who "Certified" the inspector??? Or: How crazy do we get?? ----- Original Message ----- From: "Cindy DuBois" To: "Histonet" Sent: Friday, February 16, 2007 1:57 PM Subject: [Histonet] Thermometers & CLIAA >I found it interesting that there is a discussion on thermometers going on. >We had a CLIAA inspection yesterday. Our inspector told us we weren't >"Certified" to read analog thermometers and we must use digital >thermometers for our daily temp readings of all equipment including our >refridgerator and waterbaths. > > Cindy DuBois > Integrated Pathology > Stockton, CA > > > --------------------------------- > Need a quick answer? Get one in minutes from people who know. Ask your > question on Yahoo! Answers. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From japoteete <@t> saintfrancis.com Fri Feb 16 13:56:57 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Feb 16 13:59:58 2007 Subject: [Histonet] Thermometers & CLIAA Message-ID: Did the inspector own stock in the medical equipment company selling digital thermometers? Most of us have more years of experience in reading analog thermometers as they have been around since dinosaurs roamed the earth (almost!). Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Friday, February 16, 2007 1:44 PM To: Cindy DuBois; Histonet Subject: Re: [Histonet] Thermometers & CLIAA Who "Certified" the inspector??? Or: How crazy do we get?? ----- Original Message ----- From: "Cindy DuBois" To: "Histonet" Sent: Friday, February 16, 2007 1:57 PM Subject: [Histonet] Thermometers & CLIAA >I found it interesting that there is a discussion on thermometers going >on. >We had a CLIAA inspection yesterday. Our inspector told us we weren't >"Certified" to read analog thermometers and we must use digital >thermometers for our daily temp readings of all equipment including our >refridgerator and waterbaths. > > Cindy DuBois > Integrated Pathology > Stockton, CA > > > --------------------------------- > Need a quick answer? Get one in minutes from people who know. Ask your > question on Yahoo! Answers. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Feb 16 14:13:25 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 16 14:14:00 2007 Subject: [Histonet] Thermometers & CLIAA References: Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684D44@bruexchange.digestivespecialists.com> And my children all survived even though I wasn't "certified" to read an analog thermometer! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Friday, February 16, 2007 2:57 PM To: Markus F. Meyenhofer; Cindy DuBois; Histonet Subject: RE: [Histonet] Thermometers & CLIAA Did the inspector own stock in the medical equipment company selling digital thermometers? Most of us have more years of experience in reading analog thermometers as they have been around since dinosaurs roamed the earth (almost!). Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Friday, February 16, 2007 1:44 PM To: Cindy DuBois; Histonet Subject: Re: [Histonet] Thermometers & CLIAA Who "Certified" the inspector??? Or: How crazy do we get?? ----- Original Message ----- From: "Cindy DuBois" To: "Histonet" Sent: Friday, February 16, 2007 1:57 PM Subject: [Histonet] Thermometers & CLIAA >I found it interesting that there is a discussion on thermometers going >on. >We had a CLIAA inspection yesterday. Our inspector told us we weren't >"Certified" to read analog thermometers and we must use digital >thermometers for our daily temp readings of all equipment including our >refridgerator and waterbaths. > > Cindy DuBois > Integrated Pathology > Stockton, CA > > > --------------------------------- > Need a quick answer? Get one in minutes from people who know. Ask your > question on Yahoo! Answers. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Fri Feb 16 14:19:55 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Feb 16 14:24:03 2007 Subject: [Histonet] Thermometers & CLIAA Message-ID: This reminds me of one of my CAP inspection issues. It sounds good to have accurate pipetters in the lab so, of course, another regulation shows up on pipette calibration. Now, not only to we have to calibrate our pipettes, but we have to calibrate the pipette calibrating equipment as well. Never mind the fact that actually changing the "calibration" on a pipetter that you have used for years and years will actually wind up throwing off all of your current dilutions that were made with that pipette (pre-calibration of course). I look forward to seeing what kind of new regulations are in store for the 2007 CAP inspections. Just More Regulatory Madness, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cindy DuBois Sent: Friday, February 16, 2007 12:57 PM To: Histonet Subject: [Histonet] Thermometers & CLIAA I found it interesting that there is a discussion on thermometers going on. We had a CLIAA inspection yesterday. Our inspector told us we weren't "Certified" to read analog thermometers and we must use digital thermometers for our daily temp readings of all equipment including our refridgerator and waterbaths. Cindy DuBois Integrated Pathology Stockton, CA --------------------------------- Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Feb 16 16:08:14 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Feb 16 16:12:05 2007 Subject: [Histonet] Freezing human skin biopsies for cytokine ICC In-Reply-To: Message-ID: <006501c75216$f4f4a3a0$6501a8c0@Patsy> Jamie, You might check out Peprotech.com for cytokine antibodies that work on human tissue. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jamie E Erickson Sent: Thursday, February 15, 2007 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing human skin biopsies for cytokine ICC Hello histonetters, I hope someone can help me with a question about how to freeze a human skin biopsy. I am in research but am in collaboration with a clinical lab that will be collecting human skin biopsies for me to do cytokine ICC of TNF,IFN-g, among others and possibly cell makers. My question is how do I instruct them in freezing the skin so that I get the best sample for this staining. I am going to embed the sample once it is in my lab for cryosectioning. Also if people know of manufactures of antibodies that sell TNF,IFN-g that work in human tissue that would also be helpful, I have found a few but many different protocols and fixations. Any thoughts how be helpful. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Feb 17 03:58:01 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 17 03:58:12 2007 Subject: AW: [Histonet] Freezing human skin biopsies for cytokine ICC In-Reply-To: Message-ID: <001101c7527a$1cb63760$6412a8c0@dielangs.at> First I have to admit that I have no idea how sensible these cytokines are. We do immunofluorescence on skin biopsies (immunoglobulins, fibrinogen). The skin biopsies are sent to us in an humid chamber (a tube with NaCl-humid gaze, not swimming) within 30 min after excision. We orientate and freeze them like other tissue, where frozen cuts are required: put them on the chuck, surround with OCT and let freeze at -20 degree in the cryocut. Afterwards we put two layers parafilm around the frozen chuck and store them in the refrigerator -20?C until we do the IF (within a few days). You can collect the OCT-blocks and transport them in a cryobox with dry ice. If the storage time is prolonged the temperature should proprably be at -80?. Perhaps it's an idea to let them freeze in embedding molds. It is easy to get the OCT-blocks out again. And then make little parafilm-parcels. Hope this helps Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jamie E Erickson Gesendet: Donnerstag, 15. Februar 2007 23:40 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Freezing human skin biopsies for cytokine ICC Hello histonetters, I hope someone can help me with a question about how to freeze a human skin biopsy. I am in research but am in collaboration with a clinical lab that will be collecting human skin biopsies for me to do cytokine ICC of TNF,IFN-g, among others and possibly cell makers. My question is how do I instruct them in freezing the skin so that I get the best sample for this staining. I am going to embed the sample once it is in my lab for cryosectioning. Also if people know of manufactures of antibodies that sell TNF,IFN-g that work in human tissue that would also be helpful, I have found a few but many different protocols and fixations. Any thoughts how be helpful. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Feb 18 07:25:13 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Feb 18 07:25:36 2007 Subject: [Histonet] Mortech Grossing Stations In-Reply-To: <1872B4A455B7974391609AD8034C79FC0670FB@LBEXCH01.hchd.local> Message-ID: <001c01c75360$397b0d80$fff82d4b@HPPav2> Our experience with Mopec has been very good. All of our grossing and mortuary tables are made by Mopec. Any modifications that we wanted or needed, before and after the tables arrived, Mopec was more than willing and able to accommodate. I would suggest that you communicate with them yourself, directly, rather than going through the Purchasing department. We found this works the best, as we know what we need and want, and Mopec people have always understood our "tech talk" very well. Peggy Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 16, 2007 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mortech Grossing Stations Hello to all in histoloand. Is there anyone that is using the Mortech grossing stations. We just received ours on yesterday, and there are problems already. These are not the stations that I requested. We wanted Thermo Shandon's grosslab Senior. This is what we had on capitol equipment, but in the end this is what we got. I have never heard of this company, and don't know the reliability of the equipment. If any one is using them please contact me. Thanks in advance. Allison Scott CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Mon Feb 19 04:05:33 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Feb 19 04:05:55 2007 Subject: [Histonet] Background in frozen pancreas sections Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E350B@ISS-CL-EX-V1.soton.ac.uk> Hi All, I've been doing some CD8 staining of frozen sections of mouse pancreas with autoimmune insulitis. The CD8 staining is good (using rat antiCD8 + goat anti rat:Biotin + ABC/HRP + DAB) but there is high background staining. The problem is I get a lot of brown/grey background in the exocrine cells but the islets are completely clean. I've tried numerous different blocks containing BSA, goat serum and/or mouse serum and/or Tween I've also tried using avidin/biotin block (Vector), this actually increased the background. Any suggestions? Thanks Sonya From sonya.martin <@t> soton.ac.uk Mon Feb 19 04:28:58 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Feb 19 04:29:28 2007 Subject: [Histonet] Background in frozen pancreas sections (continued...) Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E350C@ISS-CL-EX-V1.soton.ac.uk> Sorry just thought some additional info might be useful; The pancreas tissue was frozen in OCT in a bath of isopentane on dry ice, 10um sections were cut, air dried for 1hr, then fixed in acetone for 10mins. I quenched endogenous peroxidase with Pierce Peroxidase Suppressor. Sections without primary or secondary antibodies still have a littel background but its worse with the secondary (I have tried rabbit anti-rat:Biotin and it was worse). Ta From sonya.martin <@t> soton.ac.uk Mon Feb 19 05:53:04 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Feb 19 05:53:37 2007 Subject: [Histonet] Background in frozen pancreas sections References: <71437982F5B13A4D9A5B2669BDB89EE4023E350B@ISS-CL-EX-V1.soton.ac.uk> <1A7F09509ACB294B835A94B2C8EB50F402BBB837@MS-DT01VS01.tsn.tno.nl> Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E350D@ISS-CL-EX-V1.soton.ac.uk> Thanks Joost, I dilute my secondary in normal mouse serum (10%) and it has decreased the background but have not tried pre-incubating the secondary for 1hr as you suggest - will try doing this today! Thanks Sonya -----Original Message----- From: Bruijntjes, J.P. (Joost) [mailto:joost.bruijntjes@tno.nl] Sent: 19 February 2007 10:34 To: Martin S. Subject: RE: [Histonet] Background in frozen pancreas sections Hi Sonya My two cents: have you ever tried to reduced the background staining by preincubating your second antibody with normal mouse serum. I had similar problems with rat tissues incubating them with monoclonal (mouse) antibodies. But there is a strong similarity between the rat and mouse immunoglobulins. When I preincubate my secondary step for 1 hour with a certain amount of rat serum the background is vanished, and my lymphocytes are beautiful. Joost -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: maandag 19 februari 2007 11:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background in frozen pancreas sections Hi All, I've been doing some CD8 staining of frozen sections of mouse pancreas with autoimmune insulitis. The CD8 staining is good (using rat antiCD8 + goat anti rat:Biotin + ABC/HRP + DAB) but there is high background staining. The problem is I get a lot of brown/grey background in the exocrine cells but the islets are completely clean. I've tried numerous different blocks containing BSA, goat serum and/or mouse serum and/or Tween I've also tried using avidin/biotin block (Vector), this actually increased the background. Any suggestions? Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From dbpiontek <@t> hotmail.com Mon Feb 19 07:23:38 2007 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Mon Feb 19 07:24:05 2007 Subject: [Histonet] IgY Message-ID: Dear Histonet: I am looking for those out there who have worked with IgY antibodies with success. I am currently getting a significant amount background staining, despite regular reduction methods. Any recipes or tips are greatly appreciated Denise Bland-Piontek, HTL(ASCP)CTBS(AATB) NIBRI, Sr. Scientist, Pathology From anh2006 <@t> med.cornell.edu Mon Feb 19 07:55:22 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Feb 19 07:55:45 2007 Subject: [Histonet] Background in frozen pancreas sections (continued...) Message-ID: It's likely your secondary (anti-rat IgG) is cross reacting with mouse IgG in the tissue. I would suggest you purchase an anti-rat IgG which is highly cross adsorbed to many species INCLUDING mouse. This can be purchased from Jackson ImmunoResearch for example. Also, with pancreas you definitely need to do an AB block. You said it increased background? That's odd and concerning ... how are you using the kit? Lots of washes? I perform my AB block specifically after serum block and before primary (with loads of washing) and am able to get very little background. ----- Original Message ----- From: "Martin S." Date: Monday, February 19, 2007 5:28 am Subject: [Histonet] Background in frozen pancreas sections (continued...) > Sorry just thought some additional info might be useful; > > The pancreas tissue was frozen in OCT in a bath of isopentane on dry > ice, 10um sections were cut, air dried for 1hr, then fixed in acetone > for 10mins. I quenched endogenous peroxidase with Pierce Peroxidase > Suppressor. > Sections without primary or secondary antibodies still have a littel > background but its worse with the secondary (I have tried rabbit > anti-rat:Biotin and it was worse). > > Ta > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sonya.martin <@t> soton.ac.uk Mon Feb 19 10:31:03 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Feb 19 10:31:20 2007 Subject: [Histonet] Background in frozen pancreas sections (continued...) References: Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E350E@ISS-CL-EX-V1.soton.ac.uk> Thanks Andrea, My goat anti-rat is from Jackson (F(ab)2, mouse adsorbed) but I find that if I don't dilute it in 10% normal mouse serum I get a lot of background - diluted in this way it seems clean on spleen sections but maybe its different for pancreas. I used Vector's avidin/biotin blocking kit after serum block but before primary - I will try this again concentrating on the washes. Thanks again Sonya -----Original Message----- From: Andrea Hooper [mailto:anh2006@med.cornell.edu] Sent: 19 February 2007 13:55 To: Martin S. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Background in frozen pancreas sections (continued...) It's likely your secondary (anti-rat IgG) is cross reacting with mouse IgG in the tissue. I would suggest you purchase an anti-rat IgG which is highly cross adsorbed to many species INCLUDING mouse. This can be purchased from Jackson ImmunoResearch for example. Also, with pancreas you definitely need to do an AB block. You said it increased background? That's odd and concerning ... how are you using the kit? Lots of washes? I perform my AB block specifically after serum block and before primary (with loads of washing) and am able to get very little background. ----- Original Message ----- From: "Martin S." Date: Monday, February 19, 2007 5:28 am Subject: [Histonet] Background in frozen pancreas sections (continued...) > Sorry just thought some additional info might be useful; > > The pancreas tissue was frozen in OCT in a bath of isopentane on dry > ice, 10um sections were cut, air dried for 1hr, then fixed in acetone > for 10mins. I quenched endogenous peroxidase with Pierce Peroxidase > Suppressor. > Sections without primary or secondary antibodies still have a littel > background but its worse with the secondary (I have tried rabbit > anti-rat:Biotin and it was worse). > > Ta > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dorothy.L.Webb <@t> HealthPartners.Com Mon Feb 19 12:13:38 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Feb 19 12:13:49 2007 Subject: [Histonet] DECALCIFICATION Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640A52@hpes1.HealthPartners.int> I would appreciate any helpful hints on decalcification of all types of bone. We are a surgical pathology lab and receive bone from all sources. We use RDO on larger bones and sections from the jaw or skull which tend to be of greater density. We use a product called Rapid Cal Immuno from BBC Biochemical Co. for softer bones, bone marrow cores, and smaller samples or any bone that is highly suspicious of tumor for possible IHC staining. We have never had the problems that we seem to be having as of late with greater TAT on the bone samples or samples that after decalcification and processing end up very hard and problematic to section. I have looked at my variables and do not see anything that stands out as problematic in our process. We have changed nothing in our processes as of late. Any ideas or suggestions anyone?? Thanks for your time!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Anna.Inman <@t> stmarygj.org Mon Feb 19 12:50:46 2007 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Mon Feb 19 12:54:50 2007 Subject: [Histonet] Reporting Send Outs Message-ID: <2925AE271EAAD440AF48FCCEB8002D090372F58B@smgmail01.smgj.sclhs.net> On cases that require further studies at a reference lab.....do you report Flow, Cytogenetic, FISH, HPV, etc.. results on the original surgical/cytology report (with an addendum) or do you send a completely separate report with the reference lab results? Thank you for your input. Anna Inman Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JWEEMS <@t> sjha.org Mon Feb 19 13:00:05 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Feb 19 13:04:26 2007 Subject: [Histonet] Reporting Send Outs Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3451@sjhaexc02.sjha.org> We report ours separately - as a case with "R" prefix. When the pathologist looks at patient history, it is obvious there is a reference report without looking for an addendum report on the case. We have found this to work for us.. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Inman, Anna Sent: Monday, February 19, 2007 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reporting Send Outs On cases that require further studies at a reference lab.....do you report Flow, Cytogenetic, FISH, HPV, etc.. results on the original surgical/cytology report (with an addendum) or do you send a completely separate report with the reference lab results? Thank you for your input. Anna Inman Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jmahoney <@t> alegent.org Mon Feb 19 13:13:46 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Mon Feb 19 13:17:48 2007 Subject: [Histonet] Reporting Send Outs In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D090372F58B@smgmail01.smgj.sclhs.net> References: <2925AE271EAAD440AF48FCCEB8002D090372F58B@smgmail01.smgj.sclhs.net> Message-ID: <45D9A28A0200003C000058C4@gwia.alegent.org> Anna, In some cases, if the Pathologist knows the IHC will take another day to complete, he/she will issue a preliminary report and state that a final will be issued upon review of the IHC. Most of our IHC is done same day. For FISH, FLOW and Cytogenetics that take longer, we issue addendum reports. Jan Omaha >>> "Inman, Anna" 02/19/2007 12:50 PM >>> On cases that require further studies at a reference lab.....do you report Flow, Cytogenetic, FISH, HPV, etc.. results on the original surgical/cytology report (with an addendum) or do you send a completely separate report with the reference lab results? Thank you for your input. Anna Inman Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 19 14:36:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 19 14:36:33 2007 Subject: [Histonet] DECALCIFICATION In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2703640A52@hpes1.HealthPartners.int> Message-ID: <966215.26822.qm@web61219.mail.yahoo.com> Dorothy: As well as you, I used RDO for compact bones (of all types) and EDTA for delicate decalcifications (speciall bone marrow biopsies). The thing is that all our decalcifications were done with constant movement, and we changed the decalcifiers after 10 cassettes were processed. If you are also doing decalcification with movement and change the decalcifiers regularly I don't think you should have problems. Ren? J. "Webb, Dorothy L" wrote: I would appreciate any helpful hints on decalcification of all types of bone. We are a surgical pathology lab and receive bone from all sources. We use RDO on larger bones and sections from the jaw or skull which tend to be of greater density. We use a product called Rapid Cal Immuno from BBC Biochemical Co. for softer bones, bone marrow cores, and smaller samples or any bone that is highly suspicious of tumor for possible IHC staining. We have never had the problems that we seem to be having as of late with greater TAT on the bone samples or samples that after decalcification and processing end up very hard and problematic to section. I have looked at my variables and do not see anything that stands out as problematic in our process. We have changed nothing in our processes as of late. Any ideas or suggestions anyone?? Thanks for your time!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. From rjbuesa <@t> yahoo.com Mon Feb 19 14:40:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 19 14:40:51 2007 Subject: [Histonet] Reporting Send Outs In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D090372F58B@smgmail01.smgj.sclhs.net> Message-ID: <665821.81118.qm@web61221.mail.yahoo.com> Before we started doing our own FISH, the report was sent separate (because it took sometimes 2 weeks to get the results), the same for EM on kidney biopsies, otherwise any special procedure was "announced" in the original report ("Addendum about----" to follow), and when ready was issued as an addendum. Ren? J. "Inman, Anna" wrote: On cases that require further studies at a reference lab.....do you report Flow, Cytogenetic, FISH, HPV, etc.. results on the original surgical/cytology report (with an addendum) or do you send a completely separate report with the reference lab results? Thank you for your input. Anna Inman Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Have a burning question? Go to Yahoo! Answers and get answers from real people who know. From LRaff <@t> lab.uropartners.com Mon Feb 19 16:02:53 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Mon Feb 19 16:03:02 2007 Subject: [Histonet] IHC trouble shooting question Message-ID: <5DA1CA5D0B98A84985B545A24423B822052D27@UPLAB01.uplab.local> Hello to the Histonet, We are having intermittent difficulties with IHC staining. We are staining well fixed prostate core biopsies for AMACR and p63 using the PIN2 cocktail. We do a pressure cooker HIER and stain on the Dako Autostainer. We use slides that have the control tissue on the top, patient tissue on the lower 2/3rds. We are running into a condition we call "train-tracking". Looking at a core longitudinally, the center will stain, but the edges will not. The artifact is very linear, with sharp cut-off between staining and non-staining. We do not see this artifact with our CK stain, which we do not use retrieval for. Dako has been out to level the racks and check the probe tips, but the problem keeps coming back. Anyone else deal with something like this? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From plott <@t> uab.edu Mon Feb 19 16:56:33 2007 From: plott <@t> uab.edu (Patricia F Lott) Date: Mon Feb 19 17:00:35 2007 Subject: [Histonet] baby mouse whole body mounts Message-ID: <3C51FAE2D392504D9960390865494C137924D6@UABEXMB4.ad.uab.edu> Can anyone give me a protocol for whole body mounts of baby (1-day old) mouse pups? From wayneozanne <@t> sympatico.ca Mon Feb 19 17:38:30 2007 From: wayneozanne <@t> sympatico.ca (W. Ozanne) Date: Mon Feb 19 17:38:39 2007 Subject: [Histonet] IHC-CD117 (c-kit) Message-ID: We are using the Dako c-kit with the predilute antibody. We are staining on the Dako Autostainer using the Dako Target Retrieval, Envision + detection kit and DAB+ chromogen. We are experiencing inconsistent staining with the positive control slide provided with the kit as well as a known positive tissue control that we use. Today the positive control slide was negative, the known positive patient case control slide containing a previously identified GIST was very weakly positive (however the mast cells were nicely positive). Any suggestions or helpful hints from my colleagues would be so greatly appreciated. Thanks, Wayne Ozanne Chief Technologist, Pathology Department St. Joseph's Health Centre Toronto, ON From debbiekeith <@t> cox.net Mon Feb 19 19:07:19 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Feb 19 19:07:28 2007 Subject: [Histonet] Processing with Clear-Rite Message-ID: <5.2.0.9.0.20070219180515.02a743b0@pop.central.cox.net> hey all! i know someone asked this not long ago... but i can't find any responses! :) can anyone share their protocol for processing small skin biopsies using Clear-Rite in an automatic tissue processor? pros? cons? thanks in advance... :) deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.412 / Virus Database: 268.18.2/692 - Release Date: 2/18/2007 From slappycraw <@t> yahoo.com Mon Feb 19 22:11:21 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Feb 19 22:11:32 2007 Subject: [Histonet] IHC-CD117 (c-kit) In-Reply-To: Message-ID: <510476.50356.qm@web53611.mail.yahoo.com> I would order another CD117 antibody (not a predilute) and run a parallel test with both controls and then go from there. How far in advance do you cut your slides and where do you store them, may be another angle I would look at. How old is the kit? "W. Ozanne" wrote: We are using the Dako c-kit with the predilute antibody. We are staining on the Dako Autostainer using the Dako Target Retrieval, Envision + detection kit and DAB+ chromogen. We are experiencing inconsistent staining with the positive control slide provided with the kit as well as a known positive tissue control that we use. Today the positive control slide was negative, the known positive patient case control slide containing a previously identified GIST was very weakly positive (however the mast cells were nicely positive). Any suggestions or helpful hints from my colleagues would be so greatly appreciated. Thanks, Wayne Ozanne Chief Technologist, Pathology Department St. Joseph's Health Centre Toronto, ON _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From JEllin <@t> yumaregional.org Tue Feb 20 08:48:36 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Feb 20 08:48:40 2007 Subject: [Histonet] Histo tech assistant vs Technician vs technologist, HELP!!!!!!!1 Message-ID: <29BE166A2CF48D459853F8EC57CD37E88F6A3E@EXCHANGECLUSTER.yumaregional.local> I am look for help people, currently in our facility we are trying to hire for a histotech assistant in our lab. I was wondering if people would be willing to share salary ranges with me. Based on our current problem they are showing that the histotech assistant is to be paid the same as our technician job slot. This to me is the biggest problem of all. what defines assistant vs technician vs technologist. I have looked at the qualification for each one, the defining mark being certified, AAS, and BAS, but then how do you handle a perosn with 10 to 20 years of experiance with no education, just everyday savy knowledge, which to me is worth just as much as a AAS or BAS. Also what i have looked at the last ASCP wage and vacancy vs the Advance article and there is a huge discrepany in pay. HELP PLEASE!!!!!! Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From Vickroy.Jim <@t> mhsil.com Tue Feb 20 09:22:49 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Feb 20 09:29:52 2007 Subject: [Histonet] formalin at grossing stations Message-ID: We have had borderline formalin levels at one of our grossing stations and one of my techs has asked an interesting question. Currently we place cassettes of grossed tissue in baskets at the grossing station and then the cassettes are transferred to larger baskets underneath a hood. The "temporary holding baskets" at the work stations are in covered containers of formalin. The question he asked is would it cause any problems with the tissue if the "temporary holding areas" had water in them instead of formalin? My first thought is that most likely the tissue would not be appreciably affected since the small surgical biopsies are properly fixed before the grossing occurs. Storage in the temporary containers is less than a half hour. We have also heard that some folks soak their "blue sponges" in water instead of formalin in order to help the formalin levels. Of course we have tried all of the usual things to reduce formalin levels, including better ventilation, formalin neutralizing pads, etc, etc, etc. I am interested in other's thoughts and what kind of setups do they have at their work grossing stations. As a final note one of our PA's has been using water at her station for over a year and we have not seen any differences in her tissue. However she is generally working with larger tissue specimens and not small biopsies. Thanks for your comments. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From pruegg <@t> ihctech.net Tue Feb 20 09:42:40 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Feb 20 09:42:56 2007 Subject: [Histonet] formalin at grossing stations In-Reply-To: Message-ID: <200702201542.l1KFgexN059826@pro12.abac.com> Jim, We use 70% alcohol as holding reagent before grossing, but you need to make sure the tissues are really fixed before doing that or the alcohol can damage surface proteins for IHC. I would think that if you left a little formalin in the basket and used water for a short time before grossing and then continued fixing with the formalin you would be alright. How long are your samples fixed in formalin before you would want to use water for up to 30 min? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, February 20, 2007 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin at grossing stations We have had borderline formalin levels at one of our grossing stations and one of my techs has asked an interesting question. Currently we place cassettes of grossed tissue in baskets at the grossing station and then the cassettes are transferred to larger baskets underneath a hood. The "temporary holding baskets" at the work stations are in covered containers of formalin. The question he asked is would it cause any problems with the tissue if the "temporary holding areas" had water in them instead of formalin? My first thought is that most likely the tissue would not be appreciably affected since the small surgical biopsies are properly fixed before the grossing occurs. Storage in the temporary containers is less than a half hour. We have also heard that some folks soak their "blue sponges" in water instead of formalin in order to help the formalin levels. Of course we have tried all of the usual things to reduce formalin levels, including better ventilation, formalin neutralizing pads, etc, etc, etc. I am interested in other's thoughts and what kind of setups do they have at their work grossing stations. As a final note one of our PA's has been using water at her station for over a year and we have not seen any differences in her tissue. However she is generally working with larger tissue specimens and not small biopsies. Thanks for your comments. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Feb 20 09:52:58 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Feb 20 09:53:16 2007 Subject: [Histonet] Histo tech assistant vs Technician vs technologist, HELP!!!!!!!1 In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E88F6A3E@EXCHANGECLUSTER.yumaregional.local> Message-ID: <200702201553.l1KFqxOQ065463@pro12.abac.com> Jesus, I agree a person with that kind of practical experience is valuable. At the University we hired people with experience without certification at a lower rate and stipulated that they get HT certification within 1 yr then they were giving a raise and paid the same as a certified tech. with the new ASCP requirements for college AS degrees before you can take the HT exam I don't know what is going to happen to those with 20 yrs experience and no certification, I am sure someone will pay them what they are worth because of the shortage of experienced HT's but they still won't be able to gross without the degree. Since CAP does not require that HT's be certified we can have anyone do histology work. Here in Colorado I would bet that a person with 20 yrs experience in histology work would get a job and be paid as well as a certified HT even if they did not have the certification, I could be wrong about that but I don't think so. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Tuesday, February 20, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histo tech assistant vs Technician vs technologist, HELP!!!!!!!1 I am look for help people, currently in our facility we are trying to hire for a histotech assistant in our lab. I was wondering if people would be willing to share salary ranges with me. Based on our current problem they are showing that the histotech assistant is to be paid the same as our technician job slot. This to me is the biggest problem of all. what defines assistant vs technician vs technologist. I have looked at the qualification for each one, the defining mark being certified, AAS, and BAS, but then how do you handle a perosn with 10 to 20 years of experiance with no education, just everyday savy knowledge, which to me is worth just as much as a AAS or BAS. Also what i have looked at the last ASCP wage and vacancy vs the Advance article and there is a huge discrepany in pay. HELP PLEASE!!!!!! Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Feb 20 10:00:16 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Feb 20 10:00:40 2007 Subject: [Histonet] Freezing human skin biopsies for cytokine ICC In-Reply-To: <001101c7527a$1cb63760$6412a8c0@dielangs.at> Message-ID: <200702201600.l1KG0GUG069578@pro12.abac.com> Jamie, I would ask that the samples be surrounded (cryoprotected) in OCT, then snap frozen and then sent to you on dry ice. Peprotech may have some of the cytokines that may work even on ffpe tissues, on frozens for sure. We are helping them to optimize some of their antibodies usually used in Elisa/westerns to tissue. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, February 17, 2007 2:58 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Freezing human skin biopsies for cytokine ICC First I have to admit that I have no idea how sensible these cytokines are. We do immunofluorescence on skin biopsies (immunoglobulins, fibrinogen). The skin biopsies are sent to us in an humid chamber (a tube with NaCl-humid gaze, not swimming) within 30 min after excision. We orientate and freeze them like other tissue, where frozen cuts are required: put them on the chuck, surround with OCT and let freeze at -20 degree in the cryocut. Afterwards we put two layers parafilm around the frozen chuck and store them in the refrigerator -20?C until we do the IF (within a few days). You can collect the OCT-blocks and transport them in a cryobox with dry ice. If the storage time is prolonged the temperature should proprably be at -80?. Perhaps it's an idea to let them freeze in embedding molds. It is easy to get the OCT-blocks out again. And then make little parafilm-parcels. Hope this helps Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jamie E Erickson Gesendet: Donnerstag, 15. Februar 2007 23:40 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Freezing human skin biopsies for cytokine ICC Hello histonetters, I hope someone can help me with a question about how to freeze a human skin biopsy. I am in research but am in collaboration with a clinical lab that will be collecting human skin biopsies for me to do cytokine ICC of TNF,IFN-g, among others and possibly cell makers. My question is how do I instruct them in freezing the skin so that I get the best sample for this staining. I am going to embed the sample once it is in my lab for cryosectioning. Also if people know of manufactures of antibodies that sell TNF,IFN-g that work in human tissue that would also be helpful, I have found a few but many different protocols and fixations. Any thoughts how be helpful. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Feb 20 10:02:59 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Feb 20 10:03:19 2007 Subject: [Histonet] surgical brain specimens In-Reply-To: Message-ID: <200702201603.l1KG2xiw071164@pro12.abac.com> Paula, Brain sections can be difficult to keep on the slide. We drain/air dry the section really well (for hours) before baking at 60dc also for hours or overnight if possible, on plus slides of course. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conlon, Paula F. Sent: Wednesday, February 14, 2007 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surgical brain specimens Hi Histonetters, We are receiving more surgical brain sections than we have in the past. We process the specimens on the Pathos Automatic Microwave Histoprocessor and we cut the sections at 5 microns. The sections sometimes do not stay on the slides even though we use Plus slides. The Neuropathologist is not happy and she has asked me to find out if there is some way to get better sections. Thank you in advance for your help. Paula Conlon Histology Supervisor Lahey Clinic 41 Mall Road Burlington, MA 01805 781-744-1345 See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 20 10:05:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 20 10:09:44 2007 Subject: [Histonet] IHC trouble shooting question In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822052D27@UPLAB01.uplab.local> Message-ID: <660362.78908.qm@web61219.mail.yahoo.com> I would look into the surfactant (Tween 20) level (concentration). It seems that at least the Ab is not spreading adequately over the section. Just a thought! Ren? J. Lester Raff wrote: Hello to the Histonet, We are having intermittent difficulties with IHC staining. We are staining well fixed prostate core biopsies for AMACR and p63 using the PIN2 cocktail. We do a pressure cooker HIER and stain on the Dako Autostainer. We use slides that have the control tissue on the top, patient tissue on the lower 2/3rds. We are running into a condition we call "train-tracking". Looking at a core longitudinally, the center will stain, but the edges will not. The artifact is very linear, with sharp cut-off between staining and non-staining. We do not see this artifact with our CK stain, which we do not use retrieval for. Dako has been out to level the racks and check the probe tips, but the problem keeps coming back. Anyone else deal with something like this? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From eca9 <@t> georgetown.edu Tue Feb 20 10:14:08 2007 From: eca9 <@t> georgetown.edu (Eva Andersson) Date: Tue Feb 20 10:14:24 2007 Subject: [Histonet] EGFR antibody? Message-ID: <45DB1E50.2050008@georgetown.edu> Good morning, I am looking for an antibody for EGFR. Does anyone have one that works well on FFPE tissue? If you do could you please share your antibody cat.no. and company as well as your protocol. Thank you for your help, Eva From rjbuesa <@t> yahoo.com Tue Feb 20 10:15:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 20 10:19:25 2007 Subject: [Histonet] IHC-CD117 (c-kit) In-Reply-To: Message-ID: <147587.9949.qm@web61216.mail.yahoo.com> I never used prediluted antibodies. For CD117 I used the concentrated Ab from Dako, diluted 1:50 with HIER at pH6 Try to buy the concentrated Ab. Ren? J. "W. Ozanne" wrote: We are using the Dako c-kit with the predilute antibody. We are staining on the Dako Autostainer using the Dako Target Retrieval, Envision + detection kit and DAB+ chromogen. We are experiencing inconsistent staining with the positive control slide provided with the kit as well as a known positive tissue control that we use. Today the positive control slide was negative, the known positive patient case control slide containing a previously identified GIST was very weakly positive (however the mast cells were nicely positive). Any suggestions or helpful hints from my colleagues would be so greatly appreciated. Thanks, Wayne Ozanne Chief Technologist, Pathology Department St. Joseph's Health Centre Toronto, ON _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. From rjbuesa <@t> yahoo.com Tue Feb 20 10:27:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 20 10:31:34 2007 Subject: [Histonet] Histo tech assistant vs Technician vs technologist, HELP!!!!!!!1 In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E88F6A3E@EXCHANGECLUSTER.yumaregional.local> Message-ID: <244263.52875.qm@web61221.mail.yahoo.com> Jesus: Your problem is a difficult one because unfortunatelly there are no salary standards and sometimes seniority take precedent, as well as previous good evaluations determine final rates with a great dispariry amongst people sometimes doing the same job. Having said all that, at one point I was able to have approved a salary range for starting personnel, how it developed from that moment on was determined by salary increaments after the annual evaluations. The salary range was: 1-auxiliary personnel :$15/hour 2-bench histotech for general tasks: $20/hour 3-histotech for special procedures like HC and IHC: $25/hour. 4-histotech for special procedures like ISH, FISH and TEM: $30/hour. 5-supervisor and manager, being a non hourly administrative position, was negotiated with the administration (at the end the supervisor/manager ended earning less money that any of the HTs due to excess non-compensated time dedicated to administrative tasks). Hope this will help you. Ren? J. Ren? J. Jesus Ellin wrote: I am look for help people, currently in our facility we are trying to hire for a histotech assistant in our lab. I was wondering if people would be willing to share salary ranges with me. Based on our current problem they are showing that the histotech assistant is to be paid the same as our technician job slot. This to me is the biggest problem of all. what defines assistant vs technician vs technologist. I have looked at the qualification for each one, the defining mark being certified, AAS, and BAS, but then how do you handle a perosn with 10 to 20 years of experiance with no education, just everyday savy knowledge, which to me is worth just as much as a AAS or BAS. Also what i have looked at the last ASCP wage and vacancy vs the Advance article and there is a huge discrepany in pay. HELP PLEASE!!!!!! Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From eca9 <@t> georgetown.edu Tue Feb 20 09:57:02 2007 From: eca9 <@t> georgetown.edu (Eva Andersson) Date: Tue Feb 20 10:34:22 2007 Subject: [Histonet] EGFR antibody? Message-ID: <45DB1A4E.4040501@georgetown.edu> Good morning, I am looking for an antibody for EGFR. Does anyone have one that works well on FFPE tissue? If you do could you please share your antibody cat.no. and company as well as your protocol. Thank you for your help, Eva From settembr <@t> umdnj.edu Tue Feb 20 11:40:43 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Feb 20 11:41:51 2007 Subject: [Histonet] EGFR antibody? Message-ID: Hello Eva, I use Dako's EGFR on FFPE human tissue with a placenta as a control. Their cat.# is M3563. I treat with their ready-to-use Prot. K for 5 min and use EGFR at a 1:20 dilution for 15 min RT. It is for IVD use. Works well. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Eva Andersson 02/20/07 11:14 AM >>> Good morning, I am looking for an antibody for EGFR. Does anyone have one that works well on FFPE tissue? If you do could you please share your antibody cat.no. and company as well as your protocol. Thank you for your help, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From es144131 <@t> bcm.tmc.edu Tue Feb 20 11:42:04 2007 From: es144131 <@t> bcm.tmc.edu (Stephens, Elizabeth Humes) Date: Tue Feb 20 11:42:18 2007 Subject: [Histonet] non-specific red staining in Movat Message-ID: <10C24F7C4D05EB45B5F0E1B3978978490131F08F@BCMEVS7.ad.bcm.edu> 1) Does anyone else have problems with non-specific red staining on Movat? We work on heart valves which don't have muscle or fibrin, but tend to get red staining on top of the collagen. We've been decreasing the amount of time in crocein scarlet from 2 min to ~30 sec-1 min, but still have some residual staining. 2) Our collagen saffron stain also doesn't seem very strong. We've "aged" the saffron in 100% EtOH in our incubator and made sure we do extra 100% EtOH washes to make sure there's no residual water, but still don't get much of a collagen stain. I've been doing ~15 minutes in the incubator. If I do more than that my whole slide seems to turn green (we mostly get alcian blue staining in our sections) Thanks!! Elizabeth From lilbullrider00 <@t> yahoo.com Tue Feb 20 11:44:15 2007 From: lilbullrider00 <@t> yahoo.com (brent hart) Date: Tue Feb 20 11:54:35 2007 Subject: [Histonet] texas meeting info Message-ID: <276462.85218.qm@web53412.mail.yahoo.com> Good afternoon all; I hope someone out here can help. I am looking for a schedual of classes that will be held at the Houston Meeting in April. I have checked the TSH website and have had no luck. I hate to ask for this info but I need it to get founding clearence from out hospital admin. any info would be greatly appreciated thanks Brent --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From rgarhart <@t> system1.net Tue Feb 20 12:18:01 2007 From: rgarhart <@t> system1.net (Robert Garhart) Date: Tue Feb 20 12:14:31 2007 Subject: [Histonet] texas meeting info In-Reply-To: <276462.85218.qm@web53412.mail.yahoo.com> Message-ID: Try contacting Dr. Mamoun Younes at Baylor or Dr. Linda Green at the Houston VA medical center. As I understand it they have info on the Houston Society of Clinical Pathology meeting in April if that is what you are talking about. Robert Garhart Executive Recruiter System 1 Search 864.627.0012 Phone 864.627.0013 Fax 866.797.8361 Toll Free rgarhart@system1.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart Sent: Tuesday, February 20, 2007 9:44 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] texas meeting info Good afternoon all; I hope someone out here can help. I am looking for a schedual of classes that will be held at the Houston Meeting in April. I have checked the TSH website and have had no luck. I hate to ask for this info but I need it to get founding clearence from out hospital admin. any info would be greatly appreciated thanks Brent --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From am3309 <@t> uga.edu Tue Feb 20 12:21:54 2007 From: am3309 <@t> uga.edu (Abigail M. Butler) Date: Tue Feb 20 12:25:43 2007 Subject: [Histonet] (no subject) Message-ID: <20070220132154.APV51824@punts1.cc.uga.edu> I also agree with the other lady that said you may want to add Tween 20 into your wash buffer. Another thought........are you getting this effect on the control tissue only or on all of your tissue? If it's only your control it could be that your level of antigen retrieval buffer is going down and not covering your sections. Or you are letting your slides dry out somewhere. Make sure your slides are completely covered at all times. Hope you figure it out! Abbie Butler, HT (ASCP) University of Georgia College of Veterinary Medicine We are having intermittent difficulties with IHC staining. We are staining well fixed prostate core biopsies for AMACR and p63 using the PIN2 cocktail. We do a pressure cooker HIER and stain on the Dako Autostainer. We use slides that have the control tissue on the top, patient tissue on the lower 2/3rds. We are running into a condition we call "train-tracking". Looking at a core longitudinally, the center will stain, but the edges will not. The artifact is very linear, with sharp cut-off between staining and non-staining. We do not see this artifact with our CK stain, which we do not use retrieval for. Dako has been out to level the racks and check the probe tips, but the problem keeps coming back. Anyone else deal with something like this? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From gu.lang <@t> gmx.at Tue Feb 20 13:20:35 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Feb 20 13:20:39 2007 Subject: AW: [Histonet] non-specific red staining in Movat In-Reply-To: <10C24F7C4D05EB45B5F0E1B3978978490131F08F@BCMEVS7.ad.bcm.edu> Message-ID: <000301c75524$32b5b020$6412a8c0@dielangs.at> Aged collagen and collagen in tention is said to react not typical and takes up the red colour. Age: Aminogroups are oxidized and stains have no reaction partner Tension: There should be a piezzo-effect, that changes the charge of collagen. To intensify the collagen-stain, you have to prolong the phosphotungstenacid-step. That is at least true with the anilinblue-methods. Phosphotungstenacid also differentiates the red stain, especially out of the collagen. After the theories about trichromes too long staining with "big" dyes will result in over-all staining. As you have seen in green combination of alcianblue and saffron. Tell me, if that helps. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stephens, Elizabeth Humes Gesendet: Dienstag, 20. Februar 2007 18:42 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] non-specific red staining in Movat 1) Does anyone else have problems with non-specific red staining on Movat? We work on heart valves which don't have muscle or fibrin, but tend to get red staining on top of the collagen. We've been decreasing the amount of time in crocein scarlet from 2 min to ~30 sec-1 min, but still have some residual staining. 2) Our collagen saffron stain also doesn't seem very strong. We've "aged" the saffron in 100% EtOH in our incubator and made sure we do extra 100% EtOH washes to make sure there's no residual water, but still don't get much of a collagen stain. I've been doing ~15 minutes in the incubator. If I do more than that my whole slide seems to turn green (we mostly get alcian blue staining in our sections) Thanks!! Elizabeth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SBarnes <@t> elch.org Tue Feb 20 13:29:05 2007 From: SBarnes <@t> elch.org (Sue Barnes) Date: Tue Feb 20 13:29:25 2007 Subject: [Histonet] IHC for cat scratch fever Message-ID: Is anyone using the IHC for cat scratch fever on the Ventana Nexus? Would like some workup information. Have tried many variations and have not been able to get it to work. I am using the antibody from Biocare. Thanks Sue From AGrobe2555 <@t> aol.com Tue Feb 20 14:05:27 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Feb 20 14:05:52 2007 Subject: [Histonet] Heart valves and Movats Pentachrome.... Message-ID: Elizabeth, I also work with heart valves and I am confused as to your statement "that heart valves don't contain muscle", since the walls of the valves are full of smooth muscle cells (more or less depending on the valve type), and I would expect to see red staining for cytoplasm and muscle. Could you elaborate a bit? Albert


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Check out free AOL at http://free.aol.com/thenewaol/index.adp. Most comprehensive set of free safety and security tools, millions of free high-quality videos from across the web, free AOL Mail and much more. From MLashus <@t> pathgroup.com Tue Feb 20 14:49:40 2007 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Tue Feb 20 14:49:49 2007 Subject: [Histonet] Job Posting Message-ID: We are looking for a full time Pathologist Assistant. If interested please let me know. --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From Sheri.Meilus <@t> va.gov Tue Feb 20 14:59:16 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Tue Feb 20 15:00:49 2007 Subject: [Histonet] Online BS in Histotechnology Message-ID: Dear Histonetters, I've been asked by a university who has permission to offer 4 year degrees in areas where there is great need (and we are all too familiar with the shortages in our profession) to survey the Histology community regarding interest in an online opportunity to obtain a BS in Histotechnology. They would like letters expressing interest in such a program. They already have a 2 year HT program that culminates in an AS in Histotechnology. If all of you who might be interested in pursuing a BS via this route would please email me a brief (or verbose if you choose) response that you might be interested in them implementing such a program, I'll gladly forward all responses to them. You can email me privately at sheri.meilus@va.gov. S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 From FUNKM <@t> mercyhealth.com Tue Feb 20 15:01:27 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Feb 20 15:02:10 2007 Subject: [Histonet] Histo Aid Message-ID: We are looking at adding a Histology Aid, we need help with accessing in the gross room. What other duties do you have your aids help with in Histology. thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 From MLashus <@t> pathgroup.com Tue Feb 20 15:01:18 2007 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Tue Feb 20 15:05:21 2007 Subject: [Histonet] Job Posting Message-ID: Sorry, I forgot to include the information. You may email dbelew@pathgroup.com if interested. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Labs Chattanooga, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mighnon Lashus Sent: Tuesday, February 20, 2007 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Posting We are looking for a full time Pathologist Assistant. If interested please let me know. --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From FUNKM <@t> mercyhealth.com Tue Feb 20 15:07:52 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Feb 20 15:12:18 2007 Subject: [Histonet] We have had trouble with our Amcar also. Control on top and patient on bottom. Message-ID: We have had trouble with our Amcar also. Control on top and patient on bottom. We are using the red slides. I would like to get away from them because of the cost. What are you using for your IHC and specials for control slides ? Thanks marcia From Reuel.Cornelia <@t> tsrh.org Tue Feb 20 15:48:40 2007 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Tue Feb 20 15:54:37 2007 Subject: [Histonet] Thermo Excelsior vs Leica ASP300 S Message-ID: <45DB1858020000C500007C67@MAIL.TSRH.ORG> Hello histonetters, Do you have anything to share and compare the two instrument with regards to their productivity and reliability. I am caught in a dilemma of my decision which one is best as both machine have successfully shown the quality of a machine we are looking for but there must be a loop hole to distinguished the two. Your honest opinion will be highly appreciated.Please do not compare these two to other machine like VIP. I am sorry if I am putting anyone of you on this as this is my first highly priced equipment to be purchased under my care. I will based your opinion on my records so please users bring it on. Thank you very much. Reuel Cornelia Texas Scottish Rite Hospital 2222 Welborn Street Dallas, TX 75219 214-559-7766 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From Jason.PALMER <@t> svhm.org.au Tue Feb 20 16:40:26 2007 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Tue Feb 20 16:40:40 2007 Subject: [Histonet] HIF-1alpha immunohistochemistry in FFPE in mouse Message-ID: Hi. We are looking for an antibody that will label HIF-1alpha in paraffin sections in mouse tissue. Would prefer not to do mouse on mouse. There are 2 goat polyclonals I found in a search, from Santa Cruz - prodcuts sc-8711 and sc-12542. The data sheets don't mention any use with immuno's on FFPE, but a paper I recently came across uses one of them (can't be sure which one yet...) on FFPE sections of mouse pancreatic islets. Also in my searching I found a Chemicon rabbit polyclonal - AB3883 - which they claim has been used with immuno's on FFPE sections. Has anybody had success with any of these products? Any feedback greatly appreciated. We did try a Novus Biologicals monoclonal (NB 100-123) on rat tissue a few years back with little success. My impression is that HIF1-a immunostaining generally speaking is not easy to achieve... Thanks, Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From katri <@t> cogeco.ca Tue Feb 20 18:27:20 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Tue Feb 20 18:32:21 2007 Subject: [Histonet] IHC trouble shooting question References: <5DA1CA5D0B98A84985B545A24423B822052D27@UPLAB01.uplab.local> Message-ID: <004201c7554f$0cd91880$6a9a9618@Katri> Lester, We have noticed similar kind of artifact with some of the heat retrieved tissues. We seem to be able to control it by: 1. Rinsing the slides well in TBS/Tween buffer before putting them in the retrieval buffer. 2. We also add Tween in the home made retrieval buffers, commercial ones usually have a surfactant in them. If we are careless with pre-rinsing the artifact returns. The slides are very close to each other in the racks we use for retrieval. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Lester Raff" To: Sent: Monday, February 19, 2007 5:02 PM Subject: [Histonet] IHC trouble shooting question Hello to the Histonet, We are having intermittent difficulties with IHC staining. We are staining well fixed prostate core biopsies for AMACR and p63 using the PIN2 cocktail. We do a pressure cooker HIER and stain on the Dako Autostainer. We use slides that have the control tissue on the top, patient tissue on the lower 2/3rds. We are running into a condition we call "train-tracking". Looking at a core longitudinally, the center will stain, but the edges will not. The artifact is very linear, with sharp cut-off between staining and non-staining. We do not see this artifact with our CK stain, which we do not use retrieval for. Dako has been out to level the racks and check the probe tips, but the problem keeps coming back. Anyone else deal with something like this? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Feb 20 18:34:07 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Feb 20 18:37:56 2007 Subject: [Histonet] EGFR antibody? In-Reply-To: <45DB1A4E.4040501@georgetown.edu> Message-ID: <200702210034.l1L0Y7uo071798@pro12.abac.com> I use egfr from Zymed contact me and I can send you the details. We also use the pharmDx kit from Dako for some that require the FDA approved very expensive test, but for research we prefer the Zymed antibody which is way cheaper and in our hands actually better. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Andersson Sent: Tuesday, February 20, 2007 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EGFR antibody? Good morning, I am looking for an antibody for EGFR. Does anyone have one that works well on FFPE tissue? If you do could you please share your antibody cat.no. and company as well as your protocol. Thank you for your help, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From apothad <@t> gmail.com Wed Feb 21 03:40:29 2007 From: apothad <@t> gmail.com (Adam Kirk) Date: Wed Feb 21 03:40:40 2007 Subject: [Histonet] Aquaporin 2 Message-ID: <40e76ce80702210140n4ae18420lc2778e01f99dcf0a@mail.gmail.com> I NEED HELP PLEASE. I am looking at aquaporin 2 in human tissue embedded in GMA. To date I have used the standard GMA protocol with both the Abcam and Chemicon antibodies. I have tried these at 1/100 stock soltuin out to 1/1000. Then I increased this to 1/20 and di double titrations out to 1/160 top overlap with the 1/100. None of this has worked. Can anyone help? >From the papers i notice that a lot of people using aquaporin2 are producing their own antibody. I don't have the time to do this as my PhD is coming to an end quickly. So if anyone has any ideas for aquaporin in GMA or any antibody they know works on human tissue (and coul spare any), please could you get in touch. PLEASE. It would be very kind and save the ever decreasing amounts of hair I'm suffering with! Thanks Adam -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 From jnocito <@t> satx.rr.com Wed Feb 21 04:38:30 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Feb 21 04:38:39 2007 Subject: [Histonet] texas meeting info References: <276462.85218.qm@web53412.mail.yahoo.com> Message-ID: <002a01c755a4$6e6e2c20$649eae18@yourxhtr8hvc4p> Brent, The TSH meeting is being held in Houston April 20-22 Joe Nocito ----- Original Message ----- From: "brent hart" To: Sent: Tuesday, February 20, 2007 11:44 AM Subject: [Histonet] texas meeting info > Good afternoon all; > > I hope someone out here can help. I am looking for a schedual of > classes that will be held at the Houston Meeting in April. I have checked > the TSH website and have had no luck. > I hate to ask for this info but I need it to get founding clearence from > out hospital admin. > any info would be greatly appreciated > thanks > > Brent > > > > --------------------------------- > Never miss an email again! > Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Feb 21 05:38:11 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Feb 21 05:39:02 2007 Subject: [Histonet] non-specific red staining in Movat In-Reply-To: <10C24F7C4D05EB45B5F0E1B3978978490131F08F@BCMEVS7.ad.bcm.edu> Message-ID: Elizabeth, I also do Movats on a regular basis. Our Crocein Scarlet- Acid Fuschin solution is an A&B solution of 8 parts A(crocein scarlet) with 2 parts Solution B (acid fuschin). I stain for 1 1/2 min. Then rinse in DH2O 3x, followed by a rinse in 0.5% acetic acid,then 2 changes 5 min each of 5 % phosphotungstic acid followed by another rinse in 0.5% acetic acid. Rinse 3x in 100% ETOH then in Safran (0.9 gm in 100% ETOH incubated in 58 degree incubator for 48 hrs) for 15 min,rinsed quickly in 3 chages of 100%ETOH the xylene. Safran staining is not too intense, but it don't think it is meant to be. Hope this helps Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephens, Elizabeth Humes Sent: Tuesday, February 20, 2007 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-specific red staining in Movat 1) Does anyone else have problems with non-specific red staining on Movat? We work on heart valves which don't have muscle or fibrin, but tend to get red staining on top of the collagen. We've been decreasing the amount of time in crocein scarlet from 2 min to ~30 sec-1 min, but still have some residual staining. 2) Our collagen saffron stain also doesn't seem very strong. We've "aged" the saffron in 100% EtOH in our incubator and made sure we do extra 100% EtOH washes to make sure there's no residual water, but still don't get much of a collagen stain. I've been doing ~15 minutes in the incubator. If I do more than that my whole slide seems to turn green (we mostly get alcian blue staining in our sections) Thanks!! Elizabeth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Wed Feb 21 06:30:25 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Feb 21 06:30:39 2007 Subject: [Histonet] Removing "build up" from reagent containers Message-ID: Hi Histonetters, We get a "build up" on the edges of our 70% alcohol container on our stainer but no "build up" on the 70% alcohol container on the processor. (we use tap water to make up the 70%) The 1% Lithium Carbonate that we use for bluing in the frozen room also develops this "build up"? We've tried so many ways to clean these containers but have not found an effective solution. Any advice would be greatly appreciated. Have a great day!! Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Windows Live Spaces: share your New Year pictures! http://spaces.live.com/?mkt=en-ca From ASelf <@t> gmhsc.com Wed Feb 21 06:54:21 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Wed Feb 21 06:54:33 2007 Subject: [Histonet] Thermo Excelsior Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F0A1@exchange.gmhpost.com> Histonetters, I am writing for a friend of mine that doesn't have access to the histonet. She would like some feedback - good or bad - on the Thermo Excelsior Processor? Thanks in advance, Amy NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From sharon.willman <@t> bms.com Wed Feb 21 06:51:54 2007 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Wed Feb 21 06:55:33 2007 Subject: [Histonet] PASH Staining in Rat Testes Message-ID: <45DC406A.1090404@bms.com> Hi, We have successfully been doing the PASH stain in rat testes for some time. All of a sudden we cannot get our controls to stain positive. The controls are fixed in Modified Davidson's fixative. We have made new solutions, checked the ph of the water, ordered new Schiff's, and used new rat control blocks. We ran a positive colon control along with the testes control. It stained very well. Any thoughts on what might be the cause of our problem? I would appreciate your feedback. Thanks, Sharon From rjbuesa <@t> yahoo.com Wed Feb 21 07:41:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 21 08:12:17 2007 Subject: [Histonet] Histo Aid In-Reply-To: Message-ID: <263043.37310.qm@web61223.mail.yahoo.com> Marcia: I always trained my aids to take care of operating all automatic instruments in the lab, filing of all types, collating reports with slides, preparing all clerical tasks and liberating the HTs from those tasks in order to obtain a better return from the HT salary. Ren? J. Marcia Funk wrote: We are looking at adding a Histology Aid, we need help with accessing in the gross room. What other duties do you have your aids help with in Histology. thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never Miss an Email Stay connected with Yahoo! Mail on your mobile. Get started! From Susan.Walzer <@t> HCAHealthcare.com Wed Feb 21 08:16:38 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Wed Feb 21 08:20:42 2007 Subject: [Histonet] Job Opening Message-ID: <471953BC63077941B82C26A4338272B42F04FA@ORLEV03.hca.corpad.net> We are looking for a histo-tech at St Pete. General Hospital. St Pete., FL . Apply at: http://www.stpetegeneralhospital.com/ From anh2006 <@t> med.cornell.edu Wed Feb 21 09:28:39 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Feb 21 09:28:45 2007 Subject: [Histonet] oligo-1 for oligodendrocytes Message-ID: Dear Histonet, Is anyone using Oligo-1 antibody from chemicon to stain mouse brain? If so, can you please contact me as we are having some protocol problems and I wanted advice (fixative, protocol etc). Otherwise, if anyone else is using another marker to stain oligodendrocytes in mouse brain please let me know (clone, source, catalog # etc). Thanks! -- From eca9 <@t> georgetown.edu Wed Feb 21 09:29:13 2007 From: eca9 <@t> georgetown.edu (Eva Andersson) Date: Wed Feb 21 09:29:30 2007 Subject: [Histonet] SKP2 antibody problems Message-ID: <45DC6549.2080107@georgetown.edu> Hi, I have been trying to get a Skp2 antibody from Zymed (cat.no. 18-0307) to work on FFPE tissues. Anyone who have been able to get this antibody to work and who would be willing to share their protocol? If you are using another Skp2 antibody that is working I would love to hear from you too. Thanks, Eva Andersson Georgetown University From sbanwait <@t> buckinstitute.org Wed Feb 21 09:47:55 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Wed Feb 21 09:51:59 2007 Subject: [Histonet] oligo-1 for oligodendrocytes In-Reply-To: Message-ID: Hi There We use chemicon MAB1580 at a dilution of 1:200 on paraformaldehyde fixed paraffin embedded mouse brain tissue with great success. -Surita ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Wednesday, February 21, 2007 7:29 AM To: Histonet Subject: [Histonet] oligo-1 for oligodendrocytes Dear Histonet, Is anyone using Oligo-1 antibody from chemicon to stain mouse brain? If so, can you please contact me as we are having some protocol problems and I wanted advice (fixative, protocol etc). Otherwise, if anyone else is using another marker to stain oligodendrocytes in mouse brain please let me know (clone, source, catalog # etc). Thanks! -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Feb 21 10:03:10 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Feb 21 10:04:04 2007 Subject: [Histonet] Removing "build up" from reagent containers In-Reply-To: References: Message-ID: <472DF215-4A6F-43A1-840A-0C637F55962D@yahoo.com> I am curious why you are using TAP WATER instead of DI water to make your 70% alcohol. There has been a great deal of discussion regarding water in the lab arena. It's all in the WATER!! Everything is made with WATER!! Your area of the country has a tremendous amount of minerals in the water. (Lithium Carbonate) is also a mineral and brings your water to a high pH to make it a bluing solution, around pH 9.0. Besides that, there are alot of other undesirable components & Critters in TAP WATER. A residual will appear as the bluing solution with (Lithium Carbonate) stands for a period of time. This is normal and to be expected. As well as the fact that pH can change seasonally and from morning to afternoon. When I was doing research on my H&E project in 2002 & 2003, I talked to over 300 histotechs around the country. I found out that in California & Fort Bragg, NC local water providers, flush the water systems with chlorine (bleach) periodically. This is maddening when your slides are washing in tap water after staining in hematoxylin. All of a sudden your nuclear staining is stripped-out because of the chlorine content in the water. Think about it, it's doing the same thing as when you differentiate in acid alcohol. Some proactive supervisors have contacted the local water providers to give them a heads-up when they flush their system. They then wash with bottled water. Think about it, your DI water is affected too. The chlorine runs through your DI purification system. Ventana's tech support will always question the water when they are doing their trouble-shooting. They have had tons of instrument issues because of BAD water. Food for thought.... Phoenix Lab Consulting Specializing in Histology, SS, IHC, & Microarray Madison, WI Akemi Allison-Tacha BS, HT (ASCP) HTL President Cell: (925) 788-0900 E-Mail: akemiat3377@yahoo.com On Feb 21, 2007, at 6:30 AM, sheila adey wrote: > > Hi Histonetters, > > We get a "build up" on the edges of our 70% alcohol container on > our stainer but no "build up" on the 70% alcohol container on the > processor. (we use tap water to make up the 70%) > The 1% Lithium Carbonate that we use for bluing in the frozen room > also develops this "build up"? > We've tried so many ways to clean these containers but have not > found an effective solution. > Any advice would be greatly appreciated. Have a great day!! > > > Sheila Adey HT MLT > Port Huron Hospital > Michigan > > _________________________________________________________________ > Windows Live Spaces: share your New Year pictures! http:// > spaces.live.com/?mkt=en-ca > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Wed Feb 21 10:06:18 2007 From: portera <@t> msu.edu (Amy Porter) Date: Wed Feb 21 10:06:40 2007 Subject: [Histonet] Thermo Excelsior References: <39836CD6DB61654E8F95A35898C9218602E4F0A1@exchange.gmhpost.com> Message-ID: <000301c755d2$38ec1e80$8e7a0923@histolab> Have had an Excelsior in our lab for about 4-5 years - love it!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Amy Self" To: Sent: Wednesday, February 21, 2007 7:54 AM Subject: [Histonet] Thermo Excelsior Histonetters, I am writing for a friend of mine that doesn't have access to the histonet. She would like some feedback - good or bad - on the Thermo Excelsior Processor? Thanks in advance, Amy NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Wed Feb 21 10:27:08 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Feb 21 10:27:19 2007 Subject: [Histonet] Uroplakin vendor Message-ID: Could any of you please send me your vendor for Uroplakin Antibody, I'm looking for a mouse monoclonal. Our application would be for human tissue, in a clinical lab (research use only would be fine) Thanks Bec Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From JHarvey <@t> pathgroup.com Wed Feb 21 10:29:03 2007 From: JHarvey <@t> pathgroup.com (Jeff Harvey) Date: Wed Feb 21 10:29:47 2007 Subject: [Histonet] cassettes and metal lids Message-ID: We have just changed over to all plastic unicassettes and have several boxes of the old cassettes which use the metal lids. If anyone would like some of these cassettes and the metal lids we can negotiate a price. Please contact me by email at jharvey@pathgroup.com. Thank you. --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From ancillarypath <@t> mac.com Wed Feb 21 10:32:37 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Wed Feb 21 10:32:46 2007 Subject: [Histonet] Re: [IHCRG] Uroplakin vendor In-Reply-To: References: Message-ID: There you go.. Good luck with the validation. This is not a very sensitive antibody. It's good on the low-grade tumors, where it's not needed. Hadi ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. ANTIBODIES (anti-Human and others as indicated) RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. Uroplakin II clone AU1 (available as 50ug purified or 5ml Ready To Use TCS format) Mab to Uroplakin III (human/bovine) Cat#RDI-PRO651108 $345.00/vial 5ml TCS formulation cat#RDI-PRO610108 $410.00/1vial $375/vial 10+ 50ug (lyophilized) with 0.5%BSA & 0.09% NaN3 Clone AU1 Category Mouse monoclonal Immunoglobulin Class IgG1 Form/Purification Hybridoma culture supernatant Antigen Asymetric unit membrane (AUM) preparation from bovine urinary bladder Description/Specificity Mab AU1 reacts specifically with uroplakin III present in the superficial cell layer of the urothelium. Together with uroplakins UP 1A, UP 1b, and UP II, uroplakin III contributes in constituting the asymetrical unti membrane of the plaques of urothelial glycoprotein (47kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1983, 1994). Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and uretha. About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until know, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al 1995). Uroplakin III may thus serve as a specific urothelial differentiaition marker in cases of metastatic carcinomas with unclear primary tumor Antigen Recognized in Species (tested so far) Human, bovine, rat (canine reported) Application Suitable for ? immunohistochemistry on paraffin-embedded tissue (NOT ON FROZEN SECTIONS) ? immunoblotting (Western) Working Dilution Ready-to-use for immunohistochemistry (cat#RDI-PRO651108) 1:10 for histochemistry (cat#RDI-PRO610108) Incubation Time 1 h at RT for immunohistochemistry Storage At 2-8?C On Feb 21, 2007, at 11:27 AM, Orr, Rebecca wrote: > Could any of you please send me your vendor for Uroplakin Antibody, > > I?m looking for a mouse monoclonal. > > > > Our application would be for human tissue, in a clinical lab > (research use only would be fine) > > > > Thanks > > Bec > > > > Becky Orr CLA,HT(ASCP)QIHC > > Assistant Manager, Anatomic Pathology > > Evanston Northwestern Healthcare > > 847-570-2771 > > > > > --~--~---------~--~----~------------~-------~--~----~ > You received this message because you are subscribed to the Google > Groups "ihcrg" group. > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to ihcrg- > unsubscribe@googlegroups.com > For more options, visit this group at http://groups.google.com/ > group/ihcrg?hl=en > -~----------~----~----~----~------~----~------~--~--- > From mthomas <@t> littonlab.com Wed Feb 21 10:41:39 2007 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Wed Feb 21 10:41:52 2007 Subject: [Histonet] microwave processor Message-ID: <004401c755d7$28d02e10$9d35a8c0@LittonPath.local> Hello to all, We have a EBSciences model H2850 (purchased in 2002) that we are going to sell. It has a basket that will hold 64 cassettes. If anyone is interested please contact myself or Denise VanEaton off line at the number listed below. Thanks Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. From akemiat3377 <@t> yahoo.com Wed Feb 21 10:45:55 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Feb 21 10:46:04 2007 Subject: [Histonet] Removing "build up" from reagent containers In-Reply-To: <472DF215-4A6F-43A1-840A-0C637F55962D@yahoo.com> Message-ID: <927439.61107.qm@web31311.mail.mud.yahoo.com> Sorry--In the lab settings I have worked in, we would thoroughly wash and scrub the stain line and processing containers with a 5% bleach and soap mixture, followed by washing with copius amounts of tap water then DI water. Your bluing solution container most likely has a crusting residual and you may not be able to remove it if the container hasn't been scrubbed for a while. Akemi Allison-Tacha --- Akemi Allison-Tacha wrote: > I am curious why you are using TAP WATER instead of > DI water to make > your 70% alcohol. There has been a great deal of > discussion > regarding water in the lab arena. It's all in the > WATER!! > Everything is made with WATER!! Your area of the > country has a > tremendous amount of minerals in the water. (Lithium > Carbonate) is > also a mineral and brings your water to a high pH to > make it a bluing > solution, around pH 9.0. Besides that, there are > alot of other > undesirable components & Critters in TAP WATER. A > residual will > appear as the bluing solution with (Lithium > Carbonate) stands for a > period of time. This is normal and to be expected. > > As well as the fact that pH can change seasonally > and from morning to > afternoon. When I was doing research on my H&E > project in 2002 & > 2003, I talked to over 300 histotechs around the > country. I found > out that in California & Fort Bragg, NC local water > providers, flush > the water systems with chlorine (bleach) > periodically. This is > maddening when your slides are washing in tap water > after staining in > hematoxylin. All of a sudden your nuclear staining > is stripped-out > because of the chlorine content in the water. Think > about it, it's > doing the same thing as when you differentiate in > acid alcohol. Some > proactive supervisors have contacted the local water > providers to > give them a heads-up when they flush their system. > They then wash > with bottled water. Think about it, your DI water > is affected too. > The chlorine runs through your DI purification > system. Ventana's > tech support will always question the water when > they are doing their > trouble-shooting. They have had tons of instrument > issues because > of BAD water. Food for thought.... > > > Phoenix Lab Consulting > Specializing in Histology, SS, IHC, & Microarray > Madison, WI > Akemi Allison-Tacha BS, HT (ASCP) HTL > President > Cell: (925) 788-0900 > E-Mail: akemiat3377@yahoo.com > > On Feb 21, 2007, at 6:30 AM, sheila adey wrote: > > > > > Hi Histonetters, > > > > We get a "build up" on the edges of our 70% > alcohol container on > > our stainer but no "build up" on the 70% alcohol > container on the > > processor. (we use tap water to make up the 70%) > > The 1% Lithium Carbonate that we use for bluing in > the frozen room > > also develops this "build up"? > > We've tried so many ways to clean these containers > but have not > > found an effective solution. > > Any advice would be greatly appreciated. Have a > great day!! > > > > > > Sheila Adey HT MLT > > Port Huron Hospital > > Michigan > > > > > _________________________________________________________________ > > Windows Live Spaces: share your New Year pictures! > http:// > > spaces.live.com/?mkt=en-ca > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJJ <@t> Stowers-Institute.org Wed Feb 21 10:49:26 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Feb 21 10:49:54 2007 Subject: [Histonet] EM Specialist Position - Kansas City, MO Message-ID: Electron Microscopy Specialist The Stowers Institute for Medical Research has an opening for an Electron Microscopy (EM) Specialist to oversee day-to-day operations of the new electron microscopy service in the Histology Core Facility. Responsibilities include providing high quality research EM services on biological samples using established protocols; daily operation of the electron microscopy lab; using and maintaining the microscopes and ancillary equipment; assisting researchers with specimen preparation; sample preparation, including sample receipt, fixation, processing, and embedding; development of protocols; training SIMR research members and other users of techniques and equipment; development of seminars and/or presentations for internal training; purchasing supplies; and maintaining records/archives of the EM lab operation. In addition to excellent organizational, communication, and problem solving skills, the successful candidate should be familiar with operation of all applicable specimen preparation equipment and microscopes (TEM and SEM); skilled at standard specimen preparation protocols and identifying associated artifacts; have experience in high pressure freezing, cryoEM techniques, and immuno EM; be able to lift in excess of 30 pounds; and be able work overtime, including weekday, weekend, or on call work. The minimum requirements include an Associates Degree in a biological science and four years experience in research EM. Or a four year degree in a biological science and two years experience in research EM. To apply, visit: http://www.stowers-institute.org/ScientistsSought/ScientistsSought.asp#r esume From tkngflght <@t> yahoo.com Wed Feb 21 10:50:03 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Wed Feb 21 10:50:18 2007 Subject: [Histonet] Three temp openings--anyone interested? In-Reply-To: References: Message-ID: <00e101c755d8$586e77c0$6401a8c0@FSDESKTOP> Hi Everyone--Happy 'Almost Spring'! We have three open temporary travel positions left to fill--give me a call if you want to learn more about a specific opening or travel in general-- --Boston area, afternoons. Embed cut stain--nice smaller lab. Duration under discussion but probably 8 or 13 weeks --East Texas, days. Larger lab in a specialty area. Mostly cutting. 8 weeks and possible renewals. --Ohio, nights. Larger lab but easy to work in as I've benched it myself recently. Embed and cut is pretty much the job list. 13 weeks, maybe longer if you like it. To all those who have been in contact--I'm working with new software. I've not been able to send out a newsletter for a while--so that will come out hopefully soon. I'll be calling or emailing as I migrate everyone's contact information so don't hesistate to call if you are interested in one of these openings or even just to say HI ~ Thank you for your patience! Computers...can't live without 'em...too costly to drop kick out a 10th floor window, no matter how tempting. Thank you! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 800.756.3309 fax and alternate phone 281.883.7704 cell From cblack <@t> med.wayne.edu Wed Feb 21 10:55:42 2007 From: cblack <@t> med.wayne.edu (Black, Carolyn) Date: Wed Feb 21 10:59:57 2007 Subject: [Histonet] astrocytes in suspension Message-ID: <682B2B8F328A234CAEF60C4CB7D58AEA024CD17F@MED-CORE03-MS3.med.wayne.edu> Hi All, I am fairly new to using any kind of histology methods at all (engineering background) so I was hoping someone could help me out. I am interested in staining astrocytes that are in suspension with a fluorescent tag so I can then compare amounts of fluorescence between samples with a fluorometer. So, I know I would use GFAP (correct me if I'm wrong) and a fluorescent secondary would fluoresce what was tagged with GFAP. Would I have to fix these cells? If not, would I use some sort of live cell stain, like GFP? Please help me out, I'm at a loss! Thanks! Carolyn Black cblack@med.wayne.edu From kvieskaite <@t> yahoo.com Wed Feb 21 11:23:57 2007 From: kvieskaite <@t> yahoo.com (Vilma Kvieskaite) Date: Wed Feb 21 11:28:01 2007 Subject: [Histonet] About Pathcentre reagent rotation Message-ID: <708494.83608.qm@web38501.mail.mud.yahoo.com> Hi everybody who has Shandon Pathcentre! Can you tel me your experience in regents change (or rotation). How often do you that? Every x cassettes? What is your procedure? Vilma --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From Sheri.Meilus <@t> va.gov Wed Feb 21 11:49:10 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Wed Feb 21 11:53:25 2007 Subject: [Histonet] Responses to survey for BS in Histotechnology Online Message-ID: Dear Histonetters, I am receiving many positive responses from you all. Actually far too many to respond to personally, but I wanted to thank those of you who have responded, and to encourage those of you who haven't yet but might be interested, to please email me indicating your interest. I will continue to compile your emails and promise to forward all of them to the college. I'd also like to ask for some additional input from those of you who are able to find the time to help me demonstrate that the area of Histology is an "area of great need". This is a critical parameter to the college's consideration to implement the program. They want to see nationwide interest in a 4 year Histotechnology program. So, let's give it to them!! Keep your emails coming, and thank you in advance for helping to validate that there are critical shortages of Histotechs that can be addressed by the implementation of this program. Good luck to all of us! S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 From SAllen <@t> exchange.hsc.mb.ca Wed Feb 21 11:53:42 2007 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Feb 21 11:54:11 2007 Subject: [Histonet] unsubscribe Message-ID: Please delete me from Histonet. This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. From Shirley_PHUA <@t> hsa.gov.sg Wed Feb 21 12:10:02 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed Feb 21 12:10:22 2007 Subject: [Histonet] Shirley is away 21 February 2007 afternoon ... Message-ID: I will be out of the office from 21-02-2007 to 22-02-2007. I will return on 22 Februay 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From eca9 <@t> georgetown.edu Wed Feb 21 12:17:02 2007 From: eca9 <@t> georgetown.edu (Eva Andersson) Date: Wed Feb 21 12:21:29 2007 Subject: [Histonet] SKP2 antibody problems??? Message-ID: <45DC8C9E.4060202@georgetown.edu> Hi, I have been trying to get a Skp2 antibody from Zymed (cat.no. 18-0307) to work on FFPE tissues. Anyone who have been able to get this antibody to work and who would be willing to share their protocol? If you are using another Skp2 antibody that is working I would love to hear from you too. Thanks, Eva Andersson Georgetown University From katherine-walters <@t> uiowa.edu Wed Feb 21 12:38:16 2007 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Wed Feb 21 12:38:28 2007 Subject: [Histonet] laboratory microwave rates Message-ID: Hi, I am trying to determine how to recover some of the costs for our laboratory microwave. Does any one have a method for the billing of outside users for this service? Thanks for any information. Kathy From jane.moose <@t> newberryhospital.org Wed Feb 21 16:03:55 2007 From: jane.moose <@t> newberryhospital.org (Jane Moose) Date: Wed Feb 21 13:03:21 2007 Subject: [Histonet] cryostat Message-ID: <004701c75604$2defd9e0$18041f0a@LAB2> We have been approved for a new cryostat! We are small histology department and have an old-old Tissue Tek II---which still works fine. We only do a few frozen section cases a week, so not a big part of our work (2500 cases a year total). However, does anyone have any advice on which one to look at and which ones to avoid. Thanks in advance. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 803-405-7129 ************************************************************************************ This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From asmith <@t> mail.barry.edu Wed Feb 21 13:11:53 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Feb 21 13:12:03 2007 Subject: [Histonet] smooth muscle in heart valves Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E404B@exchsrv01.barrynet.barry.edu> My experience has been that unless one does the differentiatiation step under a microscope, all trichrome stains sometimes give false colors. However, if the "false color" always appears in the same place, it may not be false. Filip, Radu, & Simionescu found smooth muscle in human heart valves (Circ. Res. 59: 310-320, 1986). Their finding has been confirmed in subsequent papers from other laboratories, most recently by Borin, Vanhercke, & Weyns (Acta Cardiol. 61: 463-469, 2006) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From pruegg <@t> ihctech.net Wed Feb 21 14:08:22 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 21 14:08:36 2007 Subject: [Histonet] RAT cytokine called CINC-2beta. Message-ID: <00a001c755f4$0a56a460$6501a8c0@Patsy> Anybody done IHC on tissue using an antibody to this: RAT cytokine called CINC-2beta. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From rjbuesa <@t> yahoo.com Wed Feb 21 14:26:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 21 14:26:55 2007 Subject: [Histonet] laboratory microwave rates In-Reply-To: Message-ID: <711248.89316.qm@web61221.mail.yahoo.com> Kathy: As far as I know there is not a billing code for MW ovens, in the same way that there are no special billing codes for the different instruments used in the lab. You will recover your money when your TAT is reduced because of the MW and you can obtain more specimens processed by the same personnel in less time. Your compensation will come as a "by product" of productivity increment. Ren? J. "Walters, Katherine S" wrote: Hi, I am trying to determine how to recover some of the costs for our laboratory microwave. Does any one have a method for the billing of outside users for this service? Thanks for any information. Kathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From rjbuesa <@t> yahoo.com Wed Feb 21 14:29:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 21 14:29:41 2007 Subject: [Histonet] cryostat In-Reply-To: <004701c75604$2defd9e0$18041f0a@LAB2> Message-ID: <615952.98664.qm@web61225.mail.yahoo.com> Try to obtain a demo instrument from Leica but, since you do that few FS, consider a comprehensive overhawl of your Tissue Tek II (I like them just behind the Leica). Ren? J. Jane Moose wrote: We have been approved for a new cryostat! We are small histology department and have an old-old Tissue Tek II---which still works fine. We only do a few frozen section cases a week, so not a big part of our work (2500 cases a year total). However, does anyone have any advice on which one to look at and which ones to avoid. Thanks in advance. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 803-405-7129 ************************************************************************************ This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never Miss an Email Stay connected with Yahoo! Mail on your mobile. Get started! From Jessica <@t> medstaffservices.com Wed Feb 21 14:49:49 2007 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Feb 21 14:50:02 2007 Subject: [Histonet] Current Histology and Related Employment Opportunities Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: based on experience, very competitive Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: ~$30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable (60-80k, based on experience) Benefits: Medical, Dental, 401k 3. Generalist Hours: Monday - Friday, Day Shift Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Major Medical, Dental, Vision, 401k 3. Histology Supervisor Hours: Monday - Friday (shift to be announced) Pay: Negotiable Benefits: Major Medical, Dental, Vison, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 From Jackie.O'Connor <@t> abbott.com Wed Feb 21 14:52:17 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Feb 21 14:52:47 2007 Subject: [Histonet] Pre-filled 10% NBF containers In-Reply-To: Message-ID: Can someone give me an idea which company might sell pre-filled formalin containers that are actually filled with formalin? Most of the pre-filled containers I've been buying are only filled with 1/2 the volume of the container. I'll be happy if I can get a 60ml vial with 40 ml of formalin - 30 just isn't enough. I know, I know - 1:20. Jackie O' From doug <@t> ppspath.com Wed Feb 21 15:25:11 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Feb 21 15:22:47 2007 Subject: [Histonet] Pre-filled 10% NBF containers In-Reply-To: Message-ID: The same thing with those bags of chips! It is all air. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 21, 2007 3:52 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Pre-filled 10% NBF containers Can someone give me an idea which company might sell pre-filled formalin containers that are actually filled with formalin? Most of the pre-filled containers I've been buying are only filled with 1/2 the volume of the container. I'll be happy if I can get a 60ml vial with 40 ml of formalin - 30 just isn't enough. I know, I know - 1:20. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histomat77 <@t> hotmail.com Wed Feb 21 15:26:48 2007 From: histomat77 <@t> hotmail.com (Matt Hicks) Date: Wed Feb 21 15:27:01 2007 Subject: [Histonet] FTE Workload Message-ID: Hello to all the helpful histonetters! I am a new supervisor to histology. I have been asked a question, by my lab director, that I thought you all might be able to help me with. It deals with justification for a new FTE. What is the amount of work that can be produced by one FTE in an 8 hour period? This question deals specifically with Forensic autopsy cases, so it would include trimming of blocks and loading them on the processor for the next day, embedding, cutting, staining, slide turn-in and filing of blocks and slides. I am looking specifically for a number of blocks. Thanks in advance for all your help, Matt Hicks Supervisor Anatomic Pathology Sparrow Health System Lansing, Mi. _________________________________________________________________ Find what you need at prices you’ll love. Compare products and save at MSN® Shopping. http://shopping.msn.com/default/shp/?ptnrid=37,ptnrdata=24102&tcode=T001MSN20A0701 From dpahisto <@t> yahoo.com Wed Feb 21 15:47:20 2007 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Wed Feb 21 15:47:28 2007 Subject: [Histonet] Trying to locate.. Message-ID: <20070221214720.32177.qmail@web33413.mail.mud.yahoo.com> I am trying to locate an e-mail address for Sheron Lear. She works at University of KY? Cindy DuBois Integrated Pathology Stockton, CA --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From ploykasek <@t> phenopath.com Wed Feb 21 16:53:38 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Feb 21 16:54:11 2007 Subject: [Histonet] TX meeting Message-ID: Hi all. I would appreciate any information on the seminars being presented at the Texas State histology meeting. Thank you. Patti Loykasek PhenoPath Laboratories ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From MVaughan4 <@t> ucok.edu Wed Feb 21 16:59:42 2007 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Wed Feb 21 17:00:01 2007 Subject: [Histonet] Peripheral nerve stain In-Reply-To: Message-ID: Histonetters, I would like to stain a skin section to view nerve fibers, endings or corpuscles. Are there any specific stains that will pick up these structures in paraffin embedded, formalin-fixed tissues? One stain I have seen listed is erythrosin B and methylene blue, but I haven't seen a protocol for this stain. Any others? Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From mgolbaba <@t> uoguelph.ca Wed Feb 21 17:09:05 2007 From: mgolbaba <@t> uoguelph.ca (mgolbaba@uoguelph.ca) Date: Wed Feb 21 17:09:13 2007 Subject: [Histonet] Stain for... Message-ID: <20070221180905.6799twhrc4so4sww@webmail.uoguelph.ca> Hello everyone, I am looking for a specific stain to observe the cellulose (fibers), hemicellulose, and pectin separately in my plant specimens which are dried and ground wheat straw. I greatly appreciate if you let me know your recommended stains and their protocols. Actually, I have found several stains for lignin (e.g. phloroglucinol) and pectin not cellulose and hemicellulose but, unfortunately, they were for live tissues. Many thanks in advance. Best Regards, Mahsa ----------------------------- Mahsa Golbabaie MSc Student, Department of Plant Agriculture, University of Guelph, Canada Department of Chemical Engineering, University of Waterloo, Canada Email: mgolbaba@uoguelph.ca mgolbaba@engmail.uwaterloo.ca From ihctech2000 <@t> yahoo.com Wed Feb 21 17:47:25 2007 From: ihctech2000 <@t> yahoo.com (Sun Zhon) Date: Wed Feb 21 17:47:34 2007 Subject: [Histonet] anti-rat CD31 Message-ID: <329791.11737.qm@web50202.mail.yahoo.com> Hi All, Does anyone know whether there is a anti-rat CD31 antibody that works in FFPE tissues for IHC? Thank you in advance for the help. --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From asmith <@t> mail.barry.edu Wed Feb 21 19:18:46 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Feb 21 19:18:55 2007 Subject: [Histonet] Peripheral nerve stain In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E404C@exchsrv01.barrynet.barry.edu> Kiernan's "physical developer method" for axons (J.A. Kiernan HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., 1999, pp. 371-373)is the most sensitive stain that I know of. It will beautifully demonstrate tiny nerve endings that the Holmes method misses and the Winkelmann method just barely stains. Expect it to take several tries until you get the timing of it just right for your tissue. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MVaughan4@ucok.edu Sent: Wednesday, February 21, 2007 6:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral nerve stain Histonetters, I would like to stain a skin section to view nerve fibers, endings or corpuscles. Are there any specific stains that will pick up these structures in paraffin embedded, formalin-fixed tissues? One stain I have seen listed is erythrosin B and methylene blue, but I haven't seen a protocol for this stain. Any others? Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From stevenk <@t> med.usyd.edu.au Wed Feb 21 19:33:51 2007 From: stevenk <@t> med.usyd.edu.au (stevenk) Date: Wed Feb 21 19:32:10 2007 Subject: [Histonet] Re: Sperm DNA extraction Message-ID: Hi all I was wondering if anyone had any protocols for extracting DNA from human sperm. Thanks in advance Stephen -- |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9351 6143 |Fax:+61 2 9351 3429 From Malcolm.McCallum <@t> tamut.edu Wed Feb 21 19:57:10 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Wed Feb 21 20:01:05 2007 Subject: [Histonet] help with tumor ID References: <7DBCCC1FBC77C94F99F920D0CA6400B61E404C@exchsrv01.barrynet.barry.edu> Message-ID: Hi, I had some students doing skeletochronology on frog legs. I have a strange section that appears to be possible hyperplasia of the periosteum. It apears to be a proliferation of poorly-staining connective tissue (H-E stain). Not sure though. Anyone out there willing to take a look at a jpg and tell me what they think? VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html Spring Teaching Schedule & Office Hours: Monday: Genetics 1-2:40 pm Office Hours 4-6 pm Landscape Ecology 6-9:40 pm Tuesday Ichthyology 10-11:40 pm Office Hours/Student Research 1-2:30 pm Seminar 2:30-3:30 pm Wednesday Genetics 1-2:40 pm Office Hours/Student Research 3-5 pm Thursday Ichthyology 10-11:40 pm Office Hours/Student Research 1-4:30 pm ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Smith, Allen Sent: Wed 2/21/2007 7:18 PM To: MVaughan4@ucok.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peripheral nerve stain Kiernan's "physical developer method" for axons (J.A. Kiernan HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., 1999, pp. 371-373)is the most sensitive stain that I know of. It will beautifully demonstrate tiny nerve endings that the Holmes method misses and the Winkelmann method just barely stains. Expect it to take several tries until you get the timing of it just right for your tissue. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MVaughan4@ucok.edu Sent: Wednesday, February 21, 2007 6:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral nerve stain Histonetters, I would like to stain a skin section to view nerve fibers, endings or corpuscles. Are there any specific stains that will pick up these structures in paraffin embedded, formalin-fixed tissues? One stain I have seen listed is erythrosin B and methylene blue, but I haven't seen a protocol for this stain. Any others? Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Feb 22 02:35:47 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Feb 22 02:36:03 2007 Subject: Fw: [Histonet] cryostat Message-ID: <00bc01c7565c$73aac5a0$4724d182@ibls.gla.ac.uk> Jane, Make sure you have a look at Bright cryostats, you'll not be disappointed. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Jane Moose" To: Sent: Wednesday, February 21, 2007 10:03 PM Subject: [Histonet] cryostat We have been approved for a new cryostat! We are small histology department and have an old-old Tissue Tek II---which still works fine. We only do a few frozen section cases a week, so not a big part of our work (2500 cases a year total). However, does anyone have any advice on which one to look at and which ones to avoid. Thanks in advance. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 803-405-7129 **************************************************************************** ******** This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrcraigwbarlow <@t> googlemail.com Thu Feb 22 07:32:46 2007 From: mrcraigwbarlow <@t> googlemail.com (CRAIG BARLOW) Date: Thu Feb 22 07:32:56 2007 Subject: [Histonet] Melanin Bleaching Epitope retrieval and tissue lifting Message-ID: <22482b560702220532n6d5757f2g65fba5132fb799c4@mail.gmail.com> Dear Histonetters, Does anybody have any tips of how to keep tissue sections on the slides during heat mediated epitope retrieval after bleaching section with oxalic acid and portassium permanganate. The sections are skin and are only bleached for a matter of minutes. Any help would be welcome. Kind reagrds Craig Barlow From LSebree <@t> uwhealth.org Thu Feb 22 07:45:29 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Feb 22 07:45:42 2007 Subject: [Histonet] NeuN Message-ID: Does anyone know of a source for anti-neuronal nuclei (NeuN) antibody for FFPE tissue other than Chemicon? Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From Diane.Gladney <@t> se.amedd.army.mil Thu Feb 22 08:19:20 2007 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Thu Feb 22 08:23:49 2007 Subject: [Histonet] Thermo Excelsior vs Leica ASP300 S In-Reply-To: <46A361E17129F14087566B88D6E43D5705B39EB5@amedmlsermc133> References: <45DB1858020000C500007C67@MAIL.TSRH.ORG> <46A361E17129F14087566B88D6E43D5705B39EB5@amedmlsermc133> Message-ID: <4D55B2E997EFAE4DA6081DDE100B83020146262C@amedmlsermc133> Forgot to give you my wonderful Leica (Vashaw) representative's name. Please see revised email below. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology P.O. Box 484 4500 Stuart St. Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: Gladney, Diane C Ms MACH Sent: Wednesday, February 21, 2007 6:46 AM To: Reuel Cornelia Subject: RE: [Histonet] Thermo Excelsior vs Leica ASP300 S Reuel, We have 2 Leica ASP 300 Tissue Processors. The newest one was installed yesterday. The ease of operation and the reliability are beyond compare in my opinion. The remote fill and drain make it so easy to change out the solutions. I have been most impressed with this tissue processor. What few problems that I have had were immediately addressed and repairs made in a timely fashion. The technical support is superior and have been most helpful to me. My Leica (Vashaw) Representative, Tonia Crook, is top notch in her support and I can call on her at any time for assistance. Although the instrument reliability and ease of function is important, if you don't have the support of the company in resolving any problems, the instrument is worthless. In my opinion, you could not buy a better tissue processor. Its versatility in being able to program different protocols is a definite plus. I'm sure that you are aware of all of this if you have had the opportunity to demo this processor. Our ASP 300 has been a true work horse, easy to operate and maintain. I hope that this information is useful to you. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC? 29207 Email:? diane.gladney@se.amedd.army.mil Phone:? 803-751-2530 FAX:???? 803-751-7829 DSN:??? 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Tuesday, February 20, 2007 4:49 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Thermo Excelsior vs Leica ASP300 S Hello histonetters, Do you have anything to share and compare the two instrument with regards to their productivity and reliability. I am caught in a dilemma of my decision which one is best as both machine have successfully shown the quality of a machine we are looking for but there must be a loop hole to distinguished the two. Your honest opinion will be highly appreciated.Please do not compare these two to other machine like VIP. I am sorry if I am putting anyone of you on this as this is my first highly priced equipment to be purchased under my care. I will based your opinion on my records so please users bring it on. Thank you very much. Reuel Cornelia Texas Scottish Rite Hospital 2222 Welborn Street Dallas, TX 75219 214-559-7766 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Feb 22 08:31:15 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Feb 22 08:31:26 2007 Subject: [Histonet] Rae Staskiewicz, Please? Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB487@nmdamailsvr.nmda.ad.nmsu.edu> Rae - please "e" me at this address; I can't find your "civilian" email address and need to ask you a BVD question. Thanks! From apothad <@t> gmail.com Thu Feb 22 08:37:00 2007 From: apothad <@t> gmail.com (Adam Kirk) Date: Thu Feb 22 08:37:06 2007 Subject: [Histonet] Glycolmethacrylate and antibodies Message-ID: <40e76ce80702220637g6bd2770cjafa0ebab6698609d@mail.gmail.com> Dear histonetters, I am using aquaporin 2 antibodies that are working in paraffin but not in GMA. I understand it's a resin and its permeation into the tissue is very good. It seems in this case too good and is consequently masking the antigenic site, or reacting with the antibody to prevent its identification of the aquaporin protein. HAs anyone got any ideas to de-mask when tissue is embedded in GMA? Thanks Adam -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 From algranth <@t> u.arizona.edu Thu Feb 22 09:01:32 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Feb 22 09:01:43 2007 Subject: [Histonet] Stain for... In-Reply-To: <20070221180905.6799twhrc4so4sww@webmail.uoguelph.ca> Message-ID: <4.3.2.7.2.20070222075508.00daad68@algranth.inbox.email.arizona.edu> There is a stain protocol for pectin in Ruzin's book, Plant Microtechnique and Microscopy, page 152. The protocol indicates that it can be used for paraffin sections. There are also stains for lignin (I've done these) in the book as well. For cellulose try a PAS (Ruzin, page 148). Andi Grantham At 06:09 PM 2/21/2007 -0500, mgolbaba@uoguelph.ca wrote: >Hello everyone, > >I am looking for a specific stain to observe the cellulose (fibers), >hemicellulose, and pectin separately in my plant specimens which are >dried and ground wheat straw. >I greatly appreciate if you let me know your recommended stains and >their protocols. >Actually, I have found several stains for lignin (e.g. phloroglucinol) >and pectin not cellulose and hemicellulose but, unfortunately, they >were for live tissues. >Many thanks in advance. > >Best Regards, >Mahsa > >----------------------------- >Mahsa Golbabaie >MSc Student, >Department of Plant Agriculture, University of Guelph, Canada >Department of Chemical Engineering, University of Waterloo, Canada >Email: mgolbaba@uoguelph.ca >mgolbaba@engmail.uwaterloo.ca > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From staceylburton <@t> yahoo.com Thu Feb 22 10:21:33 2007 From: staceylburton <@t> yahoo.com (Stacey Burton) Date: Thu Feb 22 10:21:44 2007 Subject: [Histonet] New Microwave Cap Regulation Message-ID: <933773.18110.qm@web30002.mail.mud.yahoo.com> I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ____________________________________________________________________________________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 From tmmrosla <@t> healtheast.org Thu Feb 22 10:32:55 2007 From: tmmrosla <@t> healtheast.org (Mrosla, Tina M) Date: Thu Feb 22 10:35:56 2007 Subject: [Histonet] FDA approved recyclers Message-ID: <63FDBA3ACC67464B8F6672582299580601A146B6@EXCHCLUS.healtheast.loc> We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. From rjbuesa <@t> yahoo.com Thu Feb 22 11:01:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 22 11:01:33 2007 Subject: [Histonet] FTE Workload In-Reply-To: Message-ID: <455699.10215.qm@web61217.mail.yahoo.com> Matt: Under separate cover I am sending a paper of mine about staffing a histology that includes FTEs threshols, i.e. how much work for FTE can be done. Ren? J. Matt Hicks wrote: Hello to all the helpful histonetters! I am a new supervisor to histology. I have been asked a question, by my lab director, that I thought you all might be able to help me with. It deals with justification for a new FTE. What is the amount of work that can be produced by one FTE in an 8 hour period? This question deals specifically with Forensic autopsy cases, so it would include trimming of blocks and loading them on the processor for the next day, embedding, cutting, staining, slide turn-in and filing of blocks and slides. I am looking specifically for a number of blocks. Thanks in advance for all your help, Matt Hicks Supervisor Anatomic Pathology Sparrow Health System Lansing, Mi. _________________________________________________________________ Find what you need at prices you?ll love. Compare products and save at MSN? Shopping. http://shopping.msn.com/default/shp/?ptnrid=37,ptnrdata=24102&tcode=T001MSN20A0701 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From ryakay <@t> shands.ufl.edu Thu Feb 22 11:06:09 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Thu Feb 22 11:06:31 2007 Subject: [Histonet] New Microwave Cap Regulation In-Reply-To: <933773.18110.qm@web30002.mail.mud.yahoo.com> References: <933773.18110.qm@web30002.mail.mud.yahoo.com> Message-ID: <45DD8731020000D800000FB9@gw-fs1.shands.ufl.edu> Hi Stacey, If you look at ANP.27170 you will see the question "Are microwave devices used in accordance with manufacturer's instructions. I interpret this as meaning if you have a "kitchen" microwave then you should be using it for purposes in the kitchen (preparation of food). It was not intended for laboratory purposes no matter how you vent it. The note underneath states "Microwave devices should be used in accordance with manufacturer's instruction, unless CAP requirements are more stringent." We have all used these for laboratory purposes but with the new CAP requirements we have purchased a laboratory grade microwave. They are already vented. Kaye Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> Stacey Burton 2/22/2007 11:21 AM >>> I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ____________________________________________________________________________________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Feb 22 11:32:19 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Feb 22 11:32:27 2007 Subject: [Histonet] MART-1 staining Message-ID: <08A0A863637F1349BBFD83A96B27A50A11FFF7@uwhis-xchng3.uwhis.hosp.wisc.edu> Greetings all: I have recently been the lucky one to be given the task of trying to make MART-1 staining in our lab a reality. (or try anyway) I have all the protocols down for the most part. The only question I have is if I have to do any kind of enzyme digestion to open up the epitopes, etc and if so what kind. I am working on frozen skin sections. They are frozen in the cryostat and cut as soon as the tissue is brought into the lab. We are a Mohs lab, so the tissue hasn't been off the patient more than 10 minutes when we get it. I am currently trying out the Biocare prediluted MART-1, with the AP kit using Vulcan Fast Red (permanent) as a chromogen. I pH'd the buffer too before using and it was within acceptable range. So far there has been no specific staining, and only a little of what I would call background or non-specific staining along the basal layer where most of the melanocytes are located. The staining stains the entire basal layer, not individual areas where the known Melanoma areas are located. I have also tried the Innovex HMB-45 antibody with AP and fast red chromogen (aqueous) with even less of a reaction. Those sections are completely clean of any staining. Please help. I would also appreciate any other suggestions you may have. Thanks a bunch, Claire Ingles Mohs Clinic UW Madison, WI From David.Edmondson <@t> christie-tr.nwest.nhs.uk Thu Feb 22 11:39:02 2007 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Thu Feb 22 11:40:51 2007 Subject: [Histonet] Melanin Bleaching Epitope retrieval and tissue lifting References: <22482b560702220532n6d5757f2g65fba5132fb799c4@mail.gmail.com> Message-ID: <8B54F5975FC1314BBE8105F8FFC595FD2EC45A@cht-mail3-2k.xchristie.nhs.uk> Hi Craig We went for Vector's VIP alternative chromogens when, years ago we found the bleaching problem but when, out of the blue, I tried bleaching the other week the sections stayed on the DAKO ChemMate capillary gap slides and I wondered that the problem had somehow gone. At the time of problems we had been using gelatine chrome alum or Poly-lysine coated slides. So try a few coatings and see Dave Christie Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CRAIG BARLOW Sent: 22 February 2007 13:33 To: histonet Subject: [Histonet] Melanin Bleaching Epitope retrieval and tissue lifting Dear Histonetters, Does anybody have any tips of how to keep tissue sections on the slides during heat mediated epitope retrieval after bleaching section with oxalic acid and portassium permanganate. The sections are skin and are only bleached for a matter of minutes. Any help would be welcome. Kind reagrds Craig Barlow _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From rjbuesa <@t> yahoo.com Thu Feb 22 11:49:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 22 11:49:42 2007 Subject: [Histonet] FDA approved recyclers In-Reply-To: <63FDBA3ACC67464B8F6672582299580601A146B6@EXCHCLUS.healtheast.loc> Message-ID: <720711.6036.qm@web61214.mail.yahoo.com> Tina: I also used a B/R recycler for more than 10 years, we were inspected frequently in our hospital and we were never told of a FDA requirement. If that information cost you any money, ask for your money back! Ren? J. "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From POWELL_SA <@t> Mercer.edu Thu Feb 22 11:49:24 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Feb 22 11:54:14 2007 Subject: [Histonet] Meeting Reminder Message-ID: <01MDFM338NYW8WY035@Macon2.Mercer.edu> Hi Guys, Just a friendly reminder to make your reservations for GSH 's meeting in April. The programs and vendor registration forms can be downloaded from our website. The deadline for the hotel reservations is March 1st, that is next Thursday, in order to take advantage of the great rate for our meeting - $119 for single, double, triple or quad, and this is their peak season and after March 1st, the rates go up. Vendors, you can download the exhibit registration form from the symposium page. Go to www.histosearch.com/gsh and click on the GSH symposium link for complete information. Callaway Gardens GA is a beautiful place to visit this time of year. The gardens and The Day Butterfly Center are outstanding. The other attractions are the golfing, fly fishing, bass fishing, educational tours, tennis, swimming, boating, shopping, and the beautiful Sibley Horticulture Center, The Callaway Discovery Center, The Pioneer Log Cabin, The Gardens' bike and walking trails, Mr. Cason's Vegetable Garden, and all of the area's scenery and flowers. Bring your family to enjoy the Gardens. Shirley Powell GSH Secretary/Meeting Registrar From rjbuesa <@t> yahoo.com Thu Feb 22 11:55:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 22 11:55:50 2007 Subject: [Histonet] New Microwave Cap Regulation In-Reply-To: <933773.18110.qm@web30002.mail.mud.yahoo.com> Message-ID: <436636.48258.qm@web61213.mail.yahoo.com> Stacey: "Kitchen" MW ovens are designed to be used in the kitchem and at this moment any inspector will be quite "shocked" to find a kitchen MW used for laboratory tasks. They cannot be vented, although some people circumvent this issue by placing the oven inside the fumes hood. For lab tasks a lab designed (and factory prepared to be vented) oven has to be used, "no buts or ifs"! Ren? J. Stacey Burton wrote: I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ____________________________________________________________________________________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From rsrichmond <@t> aol.com Thu Feb 22 12:02:43 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Feb 22 12:02:53 2007 Subject: [Histonet] Re: cryostat In-Reply-To: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> References: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> Message-ID: <8C924C2C8E4DABE-1784-3195@MBLK-M22.sysops.aol.com> Jane Moose at Newberry County Memorial Hospital in Newberry, SC notes that she's been approved for a new cryostat. This reminds me of a reason to replace old cryostats - they use the refrigerant gas R-12, which is no longer legal in the USA (though still available) because it's a prohibited fluorocarbon. The information as to what refrigerant is used is on the base plate of the cryostat. Bob Richmond Samurai Pathologist Knoxville TN (alas - no SC medical license) ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From rsrichmond <@t> aol.com Thu Feb 22 12:09:24 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Feb 22 12:09:39 2007 Subject: [Histonet] PAS stain for nail fungus In-Reply-To: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> References: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> Message-ID: <8C924C3B79DE816-1784-31C9@MBLK-M22.sysops.aol.com> Dr. Allen Smith's recent post reminds me of a question I've been meaning to post to Histonet. Podiatrists are now performing biopsies of toenails with nail fungus, in order to get a fungal stain (PAS is being requested, I think) done. Apparently the insurance companies are now requiring a biopsy or culture diagnosis of fungal disease before they'll pay for the very expensive systemic drugs for nail fungus like terbinafine (Lamisil). Is anyone on Histonet getting these requests? What protocols do you use to cut and stain these difficult specimens? Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From ljwh2 <@t> cam.ac.uk Thu Feb 22 12:13:18 2007 From: ljwh2 <@t> cam.ac.uk (Laura Harris) Date: Thu Feb 22 12:13:53 2007 Subject: [Histonet] Stain for cerebral blood vessels Message-ID: <45DDDD3E.4000708@cam.ac.uk> Dear Histonet, I'm a molecular biologist working at the University of Cambridge, UK. I was wondering if anyone could recommend a histochemical stain that would work for cerebral blood vessels, preferably in frozen sections? I've been trying alkaline phosphatase staining but with mixed results. Would a collagen stain like Fast Green FGF work in isolation (rather than as part of a trichrome stain)? It doesn't need to be completely specific for vessels, as long as they are clearly visible. The other point is that is needs to be as quick and simple as possible, as I need to extract protein from the tissue afterwards. Immunostaining is no good for this application. All suggestions welcome as I will probably need to test several things to find one that works. Best wishes, Laura Harris From jcline <@t> wchsys.org Thu Feb 22 12:22:56 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Feb 22 12:23:07 2007 Subject: [Histonet] IHC ER/PR controls Message-ID: <000401c756ae$79576f60$1d2a14ac@wchsys.org> This question is for anyone who uses a Benchmark XT. We would like to know at what percentages your pathologists prefer their controls. 11-25% weak staining or do they prefer the ER/PR controls with a stronger percentage? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From LSebree <@t> uwhealth.org Thu Feb 22 12:35:47 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Feb 22 12:35:54 2007 Subject: [Histonet] IHC ER/PR controls In-Reply-To: <000401c756ae$79576f60$1d2a14ac@wchsys.org> Message-ID: We usually go with stronger controls (scores of 12 out of 12 or slightly lower). This probably isn't the best; an array of intensities would be but no one is complaining. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, February 22, 2007 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC ER/PR controls This question is for anyone who uses a Benchmark XT. We would like to know at what percentages your pathologists prefer their controls. 11-25% weak staining or do they prefer the ER/PR controls with a stronger percentage? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Feb 22 12:54:53 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Feb 22 12:55:00 2007 Subject: AW: [Histonet] Stain for cerebral blood vessels In-Reply-To: <45DDDD3E.4000708@cam.ac.uk> Message-ID: <001601c756b2$f25f5590$6412a8c0@dielangs.at> I think vanGieson is the shortest collagen-stain. Staining with FGF or Anilinblue alone would perhaps lead to an overall stain, more in the collagen fibers less in the rest of the tissue. These are links to the excellent pathopic-picture-archive. Perhaps you find the right stain for your needs. HE: http://alf3.urz.unibas.ch/pathopic/getpic-fra.cfm?id=006513 PAS: http://alf3.urz.unibas.ch/pathopic/getpic-fra.cfm?id=006507 Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Laura Harris Gesendet: Donnerstag, 22. Februar 2007 19:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Stain for cerebral blood vessels Dear Histonet, I'm a molecular biologist working at the University of Cambridge, UK. I was wondering if anyone could recommend a histochemical stain that would work for cerebral blood vessels, preferably in frozen sections? I've been trying alkaline phosphatase staining but with mixed results. Would a collagen stain like Fast Green FGF work in isolation (rather than as part of a trichrome stain)? It doesn't need to be completely specific for vessels, as long as they are clearly visible. The other point is that is needs to be as quick and simple as possible, as I need to extract protein from the tissue afterwards. Immunostaining is no good for this application. All suggestions welcome as I will probably need to test several things to find one that works. Best wishes, Laura Harris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Feb 22 13:33:16 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Feb 22 13:33:28 2007 Subject: [Histonet] New Microwave Cap Regulation Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFCC5@IRMEXCH01.irm.inhs.org> Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From staceylburton <@t> yahoo.com Thu Feb 22 14:08:20 2007 From: staceylburton <@t> yahoo.com (Stacey Burton) Date: Thu Feb 22 14:08:33 2007 Subject: [Histonet] New Microwave Cap Regulation Message-ID: <537399.57004.qm@web30003.mail.mud.yahoo.com> ----- Forwarded Message ---- From: Bonnie Whitaker To: Stacey Burton Sent: Thursday, February 22, 2007 1:45:13 PM Subject: RE: [Histonet] New Microwave Cap Regulation Hi Stacey, My understanding is that this is N/A unless you are processing in your microwave, or using other "dangerous" stuff. My lab is considering this not applicable because we only use the microwave to heat water, and a few stains. We don't heat Bouins, or any other fixative, dehydrant or clearing agents. I can let you know how if this is acceptable after our inspection that could be any time from now until May. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ____________________________________________________________________________ ________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. http://autos.yahoo.com/new_cars.html From rjbuesa <@t> yahoo.com Thu Feb 22 14:18:36 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 22 14:18:45 2007 Subject: [Histonet] New Microwave Cap Regulation In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCEFCC5@IRMEXCH01.irm.inhs.org> Message-ID: <439567.55282.qm@web61214.mail.yahoo.com> Greg: The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab. To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment). Just my opinion though! Ren? J. "Luck, Greg D." wrote: Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From garciaa <@t> mskcc.org Thu Feb 22 14:25:06 2007 From: garciaa <@t> mskcc.org (garciaa@mskcc.org) Date: Thu Feb 22 14:25:25 2007 Subject: [Histonet] Dulbecco's PBS Message-ID: <597B9BC80B36084781D3951A88205F12316878@SMSKPEXMBX8.MSKCC.ROOT.MSKCC.ORG> Hi, everyone. I have a few questions I'm hoping you'll be able to help me out with. I have 10+ years doing immunohistochemistry on PFA perfused, frozen brain sections, but a few things I'd like clarified. 1) Does it matter whether one uses PBS with or without Mg and Ca for immunos? I have always done it with PBS without Mg and Ca, and have never heard of anyone doing it with Mg and Ca, but have recently encountered a scenario in which this was the only PBS available. Wondering whether this will make a difference in my staining. 2) Can anyone explain the need for dehydrating and defatting slides (after using such substrates as DAB for color development) before coverslipping? Aside from the obvious that the sections look pretty crappy without these steps, can anyone explain the science behind this? 3) Because my tissue is perfused, I typically store the brains in 30% sucrose (with a little bit of sodium azide) in 4 degrees, until I am ready to cut the blocks. At this point, brains are usually frozen in the cryostat just before cutting 40um thick sections. I have obtained wonderful staining and morphology with this technique, however I have been recently advised to store the brains embedded in OCT (or similar) in a -20 degree freezer, rather than in 4 degrees. I am skeptical and concerned that this will damage the morphology and produce that "swiss cheese" freezer artifact (you know, where there are all these holes in the tissue?) Can anyone provide any advice, insight as to the benefits and drawbacks to either/both of these methods? Thanks everyone! Any advice, insight, comments are greatly appreciated. D. Garcia ===================================================================== Please note that this e-mail and any files transmitted with it may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. From kvieskaite <@t> yahoo.com Thu Feb 22 14:25:55 2007 From: kvieskaite <@t> yahoo.com (Vilma Kvieskaite) Date: Thu Feb 22 14:26:08 2007 Subject: [Histonet] FDA approved recyclers In-Reply-To: <63FDBA3ACC67464B8F6672582299580601A146B6@EXCHCLUS.healtheast.loc> Message-ID: <20070222202555.55831.qmail@web38502.mail.mud.yahoo.com> Hallo, Maybe do you have some time to answer few questions? Do you use B/R Procycler 9700 for buffered formalin recycling? It is worth? Do you have some problems with this equipment? Do you filtrate formalin before recycling? What filters do you use? How do you make buffered formalin after recycling- maybe do you have special enclosed system for buffer preparation? Thanks, Vilma "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From rjbuesa <@t> yahoo.com Thu Feb 22 14:47:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 22 14:47:49 2007 Subject: [Histonet] FDA approved recyclers In-Reply-To: <20070222202555.55831.qmail@web38502.mail.mud.yahoo.com> Message-ID: <938619.58926.qm@web61211.mail.yahoo.com> Vilma: I never recycled formalin and let me tell you why: in the first place you BUY the neutral (buffered) formalin to avoid exposing yourself and staff to formalin. If you recycle it you defeat the whole initial purpose because at the end you will end with formalin that YOU will have to dilute, that YOU will have to neutralize and YOU will end doing what you wanted to avoid in the first place. Although it is expensive to dispose of formalin (via a specialized company) it is worth it! At least that is how I saw the whole issue and decided NOT to recycle formalin. With regards to ethanol I did not recycle it either because it takes TWICE the amount of time to recycle ethanol than to recycle xylene and is cheaper to dilute it and dispose it to the sewer system. I only recycled xylene and my B/R recycled was paid off in juts 34 months. Ren? J. Vilma Kvieskaite wrote: Hallo, Maybe do you have some time to answer few questions? Do you use B/R Procycler 9700 for buffered formalin recycling? It is worth? Do you have some problems with this equipment? Do you filtrate formalin before recycling? What filters do you use? How do you make buffered formalin after recycling- maybe do you have special enclosed system for buffer preparation? Thanks, Vilma "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. From bwhitaker <@t> brownpathology.com Thu Feb 22 15:56:21 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Feb 22 15:51:37 2007 Subject: [Histonet] FW: Microwave use Message-ID: <000a01c756cc$4993d250$3601a8c0@brownpathology.net> Well, it looks like I am wrong about being able to use my microwave to heat water, buffer, etc.... at least I checked and the Instruction Booklet does say "not for laboratory use" in the Safety Instructions portion. See a vendor's private message to me below (thanks, Donna). Bonnie Whitaker -----Original Message----- From: Donna Willis [mailto:donna@milestonemed.com] Sent: Thursday, February 22, 2007 2:52 PM To: 'Bonnie Whitaker' Subject: Microwave use Bonnie, I decided to email you direct on this issue. Your onsite inspection will be with the 2005 CAP regulations. They have changed as of Dec 2006 and your next year self inspection will be with the new regs. The regs say that you have to use the microwave according to manufacture guidelines. Household units have in their warranty that they are for food processing, do not use for laboratory use. This has always been an issue with OSHA but never mentioned in CAP. CAP has changed and added the new question. OSHA 29 CFR 1910.303(b)(2) (listed or labeled equipment shall be used or installed in accordance with any instructions included in the listing or labeling) has always been an issue but rarely enforced. As far as OSHA is concerned you should never use any piece of equipment out side of the manufacture listing. This would mean no rice steamers in the lab for AR. I have put the new regs in my workshop. I'll let you know the reaction of folks as I give them this year. Being a vendor I felt it would be better to just e-mail you myself instead of everyone on the histonet. If you feel you want to post this to the net, it is up to you. Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell From thackett <@t> ils-inc.com Thu Feb 22 16:15:22 2007 From: thackett <@t> ils-inc.com (Theleria Hackett) Date: Thu Feb 22 16:16:01 2007 Subject: [Histonet] unsubscribe Message-ID: <8C8BF3DF11143944BFD681B6FD64A51A051BDB@ILSExchange.ILS.Mail> Theleria R. Hackett, B.S., HT (ASCP)CM Program Manager, Histology Comparative Molecular Pathology Division ILS, Inc. P.O. Box 13501 Research Triangle Park, NC 27709 (919) 281-1110 ext. 730 (919) 281-1118 Fax thackett@ils-inc.com www.ils-inc.com THIS MESSAGE IS INTENDED ONLY FOR THE USE OF THE PARTY TO WHOM IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL, AND PROTECTED FROM DISCLOSURE UNDER LAW. If you are not the addressee, or a person authorized to deliver the document to the addressee, you are hereby notified that any review, disclosure, dissemination, copying, or other action based on the content of this communication is not authorized. If you have received this document in error, please immediately notify us by email or telephone. From hhernandez <@t> pathreflab.com Thu Feb 22 16:19:54 2007 From: hhernandez <@t> pathreflab.com (Hector Hernandez) Date: Thu Feb 22 16:18:18 2007 Subject: [Histonet] New Microwave Cap Regulation In-Reply-To: <439567.55282.qm@web61214.mail.yahoo.com> Message-ID: ANP.29430 Phase 1 This question doesn't state laboratory or home grade microwaves. Microwave should be placed in an appropriate ventilated hood to contain airborne chemical contaminates and potential infectious agents. Microwaves used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. This doesn't say microwave has to be laboratory grade, only the fume extractor. This checklist question doesn't apply if only non-hazardous reagents are used in the device (microwave) (e.g. water, certain biological stains) In my opinion you can use any microwave (laboratory or house microwave) as long as you can document: 1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface. 2. Periodically monitor for temperature reproducibility. 3. All containers used in the microwave are made from microwave-transparent material/ microwave proof. Don't make it any harder than it is. Hope this clears a few things, Hector -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 22, 2007 2:19 PM To: Luck, Greg D.; Stacey Burton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Microwave Cap Regulation Greg: The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab. To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment). Just my opinion though! Ren? J. "Luck, Greg D." wrote: Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Feb 22 17:03:08 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Feb 22 17:03:14 2007 Subject: [Histonet] PAS stain for nail fungus References: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> <8C924C3B79DE816-1784-31C9@MBLK-M22.sysops.aol.com> Message-ID: <00c901c756d5$9e5609d0$649eae18@yourxhtr8hvc4p> Hey Doc, you have the South Texas Toenail lab guy right here. That's why I'm known as "Joe the Toe". We soak our nails in 20% ammonium hydroxide for at least an hour before we put the blocks on the machine to process. We've been getting nailed for a couple of years and perform a PAS for Fungus on each case that we do. Reimbursement must be good, we keep getting nailed. Joe the Toe or just JTT ----- Original Message ----- From: To: Sent: Thursday, February 22, 2007 12:09 PM Subject: [Histonet] PAS stain for nail fungus > Dr. Allen Smith's recent post reminds me of a question I've been meaning > to post to Histonet. > > Podiatrists are now performing biopsies of toenails with nail fungus, in > order to get a fungal stain (PAS is being requested, I think) done. > Apparently the insurance companies are now requiring a biopsy or culture > diagnosis of fungal disease before they'll pay for the very expensive > systemic drugs for nail fungus like terbinafine (Lamisil). > > Is anyone on Histonet getting these requests? What protocols do you use to > cut and stain these difficult specimens? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and security > tools, free access to millions of high-quality videos from across the web, > free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kildee6093 <@t> mac.com Thu Feb 22 17:47:21 2007 From: kildee6093 <@t> mac.com (Marilyn McDonald) Date: Thu Feb 22 17:48:02 2007 Subject: [Histonet] LIS programs Message-ID: <2E9C4E57-F430-4180-AF78-43EE869A89B5@mac.com> What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks From akemiat3377 <@t> yahoo.com Thu Feb 22 17:51:19 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Feb 22 17:51:32 2007 Subject: [Histonet] PAS stain for nail fungus In-Reply-To: <00c901c756d5$9e5609d0$649eae18@yourxhtr8hvc4p> Message-ID: <86477.62101.qm@web31309.mail.mud.yahoo.com> Hi Joe, I guess the histology field still uses the old methods. I was taught that back in 1974 at OHSU. FYI-We also used soak in NAIR HAIR REMOVAL to soften nails. Akemi Allison-Tacha --- Joe Nocito wrote: > Hey Doc, > you have the South Texas Toenail lab guy right here. > That's why I'm known as > "Joe the Toe". > We soak our nails in 20% ammonium hydroxide for > at least an hour before > we put the blocks on the machine to process. We've > been getting nailed for a > couple of years and perform a PAS for Fungus on each > case that we do. > Reimbursement must be good, we keep getting nailed. > > Joe the Toe or just JTT > > ----- Original Message ----- > From: > To: > Sent: Thursday, February 22, 2007 12:09 PM > Subject: [Histonet] PAS stain for nail fungus > > > > Dr. Allen Smith's recent post reminds me of a > question I've been meaning > > to post to Histonet. > > > > Podiatrists are now performing biopsies of > toenails with nail fungus, in > > order to get a fungal stain (PAS is being > requested, I think) done. > > Apparently the insurance companies are now > requiring a biopsy or culture > > diagnosis of fungal disease before they'll pay for > the very expensive > > systemic drugs for nail fungus like terbinafine > (Lamisil). > > > > Is anyone on Histonet getting these requests? What > protocols do you use to > > cut and stain these difficult specimens? > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > ________________________________________________________________________ > > Check out the new AOL. Most comprehensive set of > free safety and security > > tools, free access to millions of high-quality > videos from across the web, > > free AOL Mail and more. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mc549 <@t> hotmail.com Thu Feb 22 17:56:11 2007 From: mc549 <@t> hotmail.com (M C) Date: Thu Feb 22 17:56:24 2007 Subject: [Histonet] I have an E300 Message-ID: Is anyone in the market for an ASP-300 Tissue Processor. Contact: mc549@hotmail.com for more details. -Marco Corona _________________________________________________________________ Want a degree but can't afford to quit? Top school degrees online - in as fast as 1 year http://forms.nextag.com/goto.jsp?url=/serv/main/buyer/education.jsp?doSearch=n&tm=y&search=education_text_links_88_h288c&s=4079&p=5116 From bwhitaker <@t> brownpathology.com Thu Feb 22 18:35:41 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Feb 22 18:31:00 2007 Subject: [Histonet] LIS programs In-Reply-To: <2E9C4E57-F430-4180-AF78-43EE869A89B5@mac.com> Message-ID: <000e01c756e2$8c8df660$3601a8c0@brownpathology.net> We LOVE WinSurge!! (If you are asking for Anatomic Path only). Feel free to contact me with any particular questions. Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 5:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Feb 22 18:39:54 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Feb 22 18:40:06 2007 Subject: [Histonet] PAS stain for nail fungus References: <86477.62101.qm@web31309.mail.mud.yahoo.com> Message-ID: <001001c756e3$23859370$649eae18@yourxhtr8hvc4p> we tried Nair, but we are getting results with the 20% NH3OH JTT ----- Original Message ----- From: "Akemi Allison-Tacha" To: "Joe Nocito" ; ; Sent: Thursday, February 22, 2007 5:51 PM Subject: Re: [Histonet] PAS stain for nail fungus > Hi Joe, > > I guess the histology field still uses the old > methods. I was taught that back in 1974 at OHSU. > FYI-We also used soak in NAIR HAIR REMOVAL to soften > nails. > Akemi Allison-Tacha > > > --- Joe Nocito wrote: > >> Hey Doc, >> you have the South Texas Toenail lab guy right here. >> That's why I'm known as >> "Joe the Toe". >> We soak our nails in 20% ammonium hydroxide for >> at least an hour before >> we put the blocks on the machine to process. We've >> been getting nailed for a >> couple of years and perform a PAS for Fungus on each >> case that we do. >> Reimbursement must be good, we keep getting nailed. >> >> Joe the Toe or just JTT >> >> ----- Original Message ----- >> From: >> To: >> Sent: Thursday, February 22, 2007 12:09 PM >> Subject: [Histonet] PAS stain for nail fungus >> >> >> > Dr. Allen Smith's recent post reminds me of a >> question I've been meaning >> > to post to Histonet. >> > >> > Podiatrists are now performing biopsies of >> toenails with nail fungus, in >> > order to get a fungal stain (PAS is being >> requested, I think) done. >> > Apparently the insurance companies are now >> requiring a biopsy or culture >> > diagnosis of fungal disease before they'll pay for >> the very expensive >> > systemic drugs for nail fungus like terbinafine >> (Lamisil). >> > >> > Is anyone on Histonet getting these requests? What >> protocols do you use to >> > cut and stain these difficult specimens? >> > >> > Bob Richmond >> > Samurai Pathologist >> > Knoxville TN >> > >> > ________________________________________________________________________ >> > Check out the new AOL. Most comprehensive set of >> free safety and security >> > tools, free access to millions of high-quality >> videos from across the web, >> > free AOL Mail and more. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> From lpwenk <@t> sbcglobal.net Fri Feb 23 04:59:51 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Feb 23 05:00:28 2007 Subject: [Histonet] FDA approved recyclers In-Reply-To: <720711.6036.qm@web61214.mail.yahoo.com> Message-ID: <000701c75739$bea957f0$86f62d4b@HPPav2> Going to put in my 2 cents, which may not be too accurate. The FDA (Food and Drug Administration) covers topics that relate to Food and Drugs and medical devices that go INTO a HUMAN BEING, and anything else that touches these foods and drugs and medical devices that could effect a person's health. So as far as "recycling" would go, the FDA would be concerned about food or drugs that were being gathered after NOT being used and "recycled" for reuse (think of drugs after expiration date, or that had already been given to a patient and returned back to pharmacy). The FDA is also concerned about the plastic that goes around the food and drugs, as I remember they were concerned about new "recycled" plastic that the food and drugs are packaged in, and whether recycled plastic emit anything that would compromise the food or drugs and make it a health hazard. I know the FDA was concerned about medical devices (artificial hips, pace makers, etc.) being removed from people, cleaned up, and "recycled" for use in another person. As for a xylene/alcohol/formalin recylcer - I personally think this is out of the jurisdication of the FDA. The equipment itself does not go inside a person, nor is the intended use of these chemicals that are being recycled intended to human consumption. Neither the recycler nor the chemicals are intended for human USE. On the other hand, - OSHA has lots laws about chemical exposure to people that would apply - EPA would be concerned if the recycled chemicals got into the atmosphere or the water, and care about the proper disposal of waste - CAP has checklist questions for safety and use of equipment, electricity, chemicals, disposal, etc. that would apply. - I've never read the GLP, put I'm certain they have similar rules about chemicals and equipment. - Local fire marshals and the hospital's insurance company may have their own concern, especially as to where the recycler is positioned - but this is for scenarios of catching on fire and explosions. If someone knows of the FDA's concern in recycling chemicals in the laboratory, please inform HistoNet. Thanks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 22, 2007 12:50 PM To: Mrosla, Tina M; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FDA approved recyclers Tina: I also used a B/R recycler for more than 10 years, we were inspected frequently in our hospital and we were never told of a FDA requirement. If that information cost you any money, ask for your money back! Ren? J. "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Feb 23 05:24:22 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 23 05:24:52 2007 Subject: [Histonet] LIS programs Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D34F6@sjhaexc02.sjha.org> Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP programs. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From debbiekeith <@t> cox.net Fri Feb 23 07:25:22 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Fri Feb 23 07:25:33 2007 Subject: [Histonet] specimen clamps Message-ID: <5.2.0.9.0.20070223062151.02ca7ca8@pop.central.cox.net> hey! i have a Reichert-Jung 820 that has a screw specimen clamp and would like to replace it with a quick-release version. is the accu-edge universal quick-release adapter compatible? deb :) -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.412 / Virus Database: 268.18.3/697 - Release Date: 2/22/2007 From KMH.02 <@t> ex.uchs.org Fri Feb 23 07:26:03 2007 From: KMH.02 <@t> ex.uchs.org (Hopkins, Karen) Date: Fri Feb 23 07:26:34 2007 Subject: [Histonet] toenails In-Reply-To: Message-ID: <640B23A5DC4B234BB065E56F2DB30596028AB48A@uchex2ucmc.uchs.org> We used to cut toenails by the hundreds and all we ever did was process on a routine processing cycle then embed as usual but on edge horizontally in the cassette. We put albumin on our slides to hold the nail on and stained them routinely. They were no problem once you got used to them. Also, I agree with Hector concerning microwaves- I do as he suggested. Karen Hopkins Histology Supervisor Upper Chesapeake Medical Center & Harford Memorial Hospital p. 443-643-1454 f. 443-643-1450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 23, 2007 6:26 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 39, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: cryostat (rsrichmond@aol.com) 2. PAS stain for nail fungus (rsrichmond@aol.com) 3. Stain for cerebral blood vessels (Laura Harris) 4. IHC ER/PR controls (Joyce Cline) 5. RE: IHC ER/PR controls (Sebree Linda A.) 6. AW: [Histonet] Stain for cerebral blood vessels (Gudrun Lang) 7. RE: New Microwave Cap Regulation (Luck, Greg D.) 8. New Microwave Cap Regulation (Stacey Burton) 9. RE: New Microwave Cap Regulation (Rene J Buesa) 10. Dulbecco's PBS (garciaa@mskcc.org) 11. Re: FDA approved recyclers (Vilma Kvieskaite) 12. Re: FDA approved recyclers (Rene J Buesa) 13. FW: Microwave use (Bonnie Whitaker) 14. unsubscribe (Theleria Hackett) 15. RE: New Microwave Cap Regulation (Hector Hernandez) 16. Re: PAS stain for nail fungus (Joe Nocito) 17. LIS programs (Marilyn McDonald) 18. Re: PAS stain for nail fungus (Akemi Allison-Tacha) 19. I have an E300 (M C) 20. RE: LIS programs (Bonnie Whitaker) 21. Re: PAS stain for nail fungus (Joe Nocito) 22. RE: FDA approved recyclers (Lee & Peggy Wenk) 23. RE: LIS programs (Weems, Joyce) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 Feb 2007 13:02:43 -0500 From: rsrichmond@aol.com Subject: [Histonet] Re: cryostat To: histonet@lists.utsouthwestern.edu Message-ID: <8C924C2C8E4DABE-1784-3195@MBLK-M22.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Jane Moose at Newberry County Memorial Hospital in Newberry, SC notes that she's been approved for a new cryostat. This reminds me of a reason to replace old cryostats - they use the refrigerant gas R-12, which is no longer legal in the USA (though still available) because it's a prohibited fluorocarbon. The information as to what refrigerant is used is on the base plate of the cryostat. Bob Richmond Samurai Pathologist Knoxville TN (alas - no SC medical license) ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. ------------------------------ Message: 2 Date: Thu, 22 Feb 2007 13:09:24 -0500 From: rsrichmond@aol.com Subject: [Histonet] PAS stain for nail fungus To: histonet@lists.utsouthwestern.edu Message-ID: <8C924C3B79DE816-1784-31C9@MBLK-M22.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Dr. Allen Smith's recent post reminds me of a question I've been meaning to post to Histonet. Podiatrists are now performing biopsies of toenails with nail fungus, in order to get a fungal stain (PAS is being requested, I think) done. Apparently the insurance companies are now requiring a biopsy or culture diagnosis of fungal disease before they'll pay for the very expensive systemic drugs for nail fungus like terbinafine (Lamisil). Is anyone on Histonet getting these requests? What protocols do you use to cut and stain these difficult specimens? Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. ------------------------------ Message: 3 Date: Thu, 22 Feb 2007 18:13:18 +0000 From: Laura Harris Subject: [Histonet] Stain for cerebral blood vessels To: histonet@lists.utsouthwestern.edu Message-ID: <45DDDD3E.4000708@cam.ac.uk> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Histonet, I'm a molecular biologist working at the University of Cambridge, UK. I was wondering if anyone could recommend a histochemical stain that would work for cerebral blood vessels, preferably in frozen sections? I've been trying alkaline phosphatase staining but with mixed results. Would a collagen stain like Fast Green FGF work in isolation (rather than as part of a trichrome stain)? It doesn't need to be completely specific for vessels, as long as they are clearly visible. The other point is that is needs to be as quick and simple as possible, as I need to extract protein from the tissue afterwards. Immunostaining is no good for this application. All suggestions welcome as I will probably need to test several things to find one that works. Best wishes, Laura Harris ------------------------------ Message: 4 Date: Thu, 22 Feb 2007 13:22:56 -0500 From: "Joyce Cline" Subject: [Histonet] IHC ER/PR controls To: Message-ID: <000401c756ae$79576f60$1d2a14ac@wchsys.org> Content-Type: text/plain;charset="us-ascii" This question is for anyone who uses a Benchmark XT. We would like to know at what percentages your pathologists prefer their controls. 11-25% weak staining or do they prefer the ER/PR controls with a stronger percentage? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 5 Date: Thu, 22 Feb 2007 12:35:47 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] IHC ER/PR controls To: "Joyce Cline" , Message-ID: Content-Type: text/plain; charset="us-ascii" We usually go with stronger controls (scores of 12 out of 12 or slightly lower). This probably isn't the best; an array of intensities would be but no one is complaining. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, February 22, 2007 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC ER/PR controls This question is for anyone who uses a Benchmark XT. We would like to know at what percentages your pathologists prefer their controls. 11-25% weak staining or do they prefer the ER/PR controls with a stronger percentage? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 22 Feb 2007 19:54:53 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Stain for cerebral blood vessels To: Message-ID: <001601c756b2$f25f5590$6412a8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I think vanGieson is the shortest collagen-stain. Staining with FGF or Anilinblue alone would perhaps lead to an overall stain, more in the collagen fibers less in the rest of the tissue. These are links to the excellent pathopic-picture-archive. Perhaps you find the right stain for your needs. HE: http://alf3.urz.unibas.ch/pathopic/getpic-fra.cfm?id=006513 PAS: http://alf3.urz.unibas.ch/pathopic/getpic-fra.cfm?id=006507 Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Laura Harris Gesendet: Donnerstag, 22. Februar 2007 19:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Stain for cerebral blood vessels Dear Histonet, I'm a molecular biologist working at the University of Cambridge, UK. I was wondering if anyone could recommend a histochemical stain that would work for cerebral blood vessels, preferably in frozen sections? I've been trying alkaline phosphatase staining but with mixed results. Would a collagen stain like Fast Green FGF work in isolation (rather than as part of a trichrome stain)? It doesn't need to be completely specific for vessels, as long as they are clearly visible. The other point is that is needs to be as quick and simple as possible, as I need to extract protein from the tissue afterwards. Immunostaining is no good for this application. All suggestions welcome as I will probably need to test several things to find one that works. Best wishes, Laura Harris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 22 Feb 2007 11:33:16 -0800 From: "Luck, Greg D." Subject: RE: [Histonet] New Microwave Cap Regulation To: "Stacey Burton" , Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFCC5@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="us-ascii" Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 22 Feb 2007 12:08:20 -0800 (PST) From: Stacey Burton Subject: [Histonet] New Microwave Cap Regulation To: Histonet Listserver Message-ID: <537399.57004.qm@web30003.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ----- Forwarded Message ---- From: Bonnie Whitaker To: Stacey Burton Sent: Thursday, February 22, 2007 1:45:13 PM Subject: RE: [Histonet] New Microwave Cap Regulation Hi Stacey, My understanding is that this is N/A unless you are processing in your microwave, or using other "dangerous" stuff. My lab is considering this not applicable because we only use the microwave to heat water, and a few stains. We don't heat Bouins, or any other fixative, dehydrant or clearing agents. I can let you know how if this is acceptable after our inspection that could be any time from now until May. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ____________________________________________________________________________ ________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. http://autos.yahoo.com/new_cars.html ------------------------------ Message: 9 Date: Thu, 22 Feb 2007 12:18:36 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] New Microwave Cap Regulation To: "Luck, Greg D." , Stacey Burton , histonet@lists.utsouthwestern.edu Message-ID: <439567.55282.qm@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Greg: The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab. To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment). Just my opinion though! Ren? J. "Luck, Greg D." wrote: Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. ------------------------------ Message: 10 Date: Thu, 22 Feb 2007 15:25:06 -0500 From: garciaa@mskcc.org Subject: [Histonet] Dulbecco's PBS To: histonet@lists.utsouthwestern.edu Message-ID: <597B9BC80B36084781D3951A88205F12316878@SMSKPEXMBX8.MSKCC.ROOT.MSKCC.ORG> Content-Type: text/plain; charset=iso-8859-1 Hi, everyone. I have a few questions I'm hoping you'll be able to help me out with. I have 10+ years doing immunohistochemistry on PFA perfused, frozen brain sections, but a few things I'd like clarified. 1) Does it matter whether one uses PBS with or without Mg and Ca for immunos? I have always done it with PBS without Mg and Ca, and have never heard of anyone doing it with Mg and Ca, but have recently encountered a scenario in which this was the only PBS available. Wondering whether this will make a difference in my staining. 2) Can anyone explain the need for dehydrating and defatting slides (after using such substrates as DAB for color development) before coverslipping? Aside from the obvious that the sections look pretty crappy without these steps, can anyone explain the science behind this? 3) Because my tissue is perfused, I typically store the brains in 30% sucrose (with a little bit of sodium azide) in 4 degrees, until I am ready to cut the blocks. At this point, brains are usually frozen in the cryostat just before cutting 40um thick sections. I have obtained wonderful staining and morphology with this technique, however I have been recently advised to store the brains embedded in OCT (or similar) in a -20 degree freezer, rather than in 4 degrees. I am skeptical and concerned that this will damage the morphology and produce that "swiss cheese" freezer artifact (you know, where there are all these holes in the tissue?) Can anyone provide any advice, insight as to the benefits and drawbacks to either/both of these methods? Thanks everyone! Any advice, insight, comments are greatly appreciated. D. Garcia ===================================================================== Please note that this e-mail and any files transmitted with it may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. ------------------------------ Message: 11 Date: Thu, 22 Feb 2007 12:25:55 -0800 (PST) From: Vilma Kvieskaite Subject: Re: [Histonet] FDA approved recyclers To: histonet@lists.utsouthwestern.edu Cc: tmmrosla@healtheast.org Message-ID: <20070222202555.55831.qmail@web38502.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hallo, Maybe do you have some time to answer few questions? Do you use B/R Procycler 9700 for buffered formalin recycling? It is worth? Do you have some problems with this equipment? Do you filtrate formalin before recycling? What filters do you use? How do you make buffered formalin after recycling- maybe do you have special enclosed system for buffer preparation? Thanks, Vilma "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. ------------------------------ Message: 12 Date: Thu, 22 Feb 2007 12:47:42 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] FDA approved recyclers To: Vilma Kvieskaite , histonet@lists.utsouthwestern.edu Cc: tmmrosla@healtheast.org Message-ID: <938619.58926.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Vilma: I never recycled formalin and let me tell you why: in the first place you BUY the neutral (buffered) formalin to avoid exposing yourself and staff to formalin. If you recycle it you defeat the whole initial purpose because at the end you will end with formalin that YOU will have to dilute, that YOU will have to neutralize and YOU will end doing what you wanted to avoid in the first place. Although it is expensive to dispose of formalin (via a specialized company) it is worth it! At least that is how I saw the whole issue and decided NOT to recycle formalin. With regards to ethanol I did not recycle it either because it takes TWICE the amount of time to recycle ethanol than to recycle xylene and is cheaper to dilute it and dispose it to the sewer system. I only recycled xylene and my B/R recycled was paid off in juts 34 months. Ren? J. Vilma Kvieskaite wrote: Hallo, Maybe do you have some time to answer few questions? Do you use B/R Procycler 9700 for buffered formalin recycling? It is worth? Do you have some problems with this equipment? Do you filtrate formalin before recycling? What filters do you use? How do you make buffered formalin after recycling- maybe do you have special enclosed system for buffer preparation? Thanks, Vilma "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. ------------------------------ Message: 13 Date: Thu, 22 Feb 2007 15:56:21 -0600 From: "Bonnie Whitaker" Subject: [Histonet] FW: Microwave use To: Cc: staceylburton@yahoo.com Message-ID: <000a01c756cc$4993d250$3601a8c0@brownpathology.net> Content-Type: text/plain; charset="us-ascii" Well, it looks like I am wrong about being able to use my microwave to heat water, buffer, etc.... at least I checked and the Instruction Booklet does say "not for laboratory use" in the Safety Instructions portion. See a vendor's private message to me below (thanks, Donna). Bonnie Whitaker -----Original Message----- From: Donna Willis [mailto:donna@milestonemed.com] Sent: Thursday, February 22, 2007 2:52 PM To: 'Bonnie Whitaker' Subject: Microwave use Bonnie, I decided to email you direct on this issue. Your onsite inspection will be with the 2005 CAP regulations. They have changed as of Dec 2006 and your next year self inspection will be with the new regs. The regs say that you have to use the microwave according to manufacture guidelines. Household units have in their warranty that they are for food processing, do not use for laboratory use. This has always been an issue with OSHA but never mentioned in CAP. CAP has changed and added the new question. OSHA 29 CFR 1910.303(b)(2) (listed or labeled equipment shall be used or installed in accordance with any instructions included in the listing or labeling) has always been an issue but rarely enforced. As far as OSHA is concerned you should never use any piece of equipment out side of the manufacture listing. This would mean no rice steamers in the lab for AR. I have put the new regs in my workshop. I'll let you know the reaction of folks as I give them this year. Being a vendor I felt it would be better to just e-mail you myself instead of everyone on the histonet. If you feel you want to post this to the net, it is up to you. Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell ------------------------------ Message: 14 Date: Thu, 22 Feb 2007 17:15:22 -0500 From: "Theleria Hackett" Subject: [Histonet] unsubscribe To: Message-ID: <8C8BF3DF11143944BFD681B6FD64A51A051BDB@ILSExchange.ILS.Mail> Content-Type: text/plain; charset="iso-8859-1" Theleria R. Hackett, B.S., HT (ASCP)CM Program Manager, Histology Comparative Molecular Pathology Division ILS, Inc. P.O. Box 13501 Research Triangle Park, NC 27709 (919) 281-1110 ext. 730 (919) 281-1118 Fax thackett@ils-inc.com www.ils-inc.com THIS MESSAGE IS INTENDED ONLY FOR THE USE OF THE PARTY TO WHOM IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL, AND PROTECTED FROM DISCLOSURE UNDER LAW. If you are not the addressee, or a person authorized to deliver the document to the addressee, you are hereby notified that any review, disclosure, dissemination, copying, or other action based on the content of this communication is not authorized. If you have received this document in error, please immediately notify us by email or telephone. ------------------------------ Message: 15 Date: Thu, 22 Feb 2007 16:19:54 -0600 From: "Hector Hernandez" Subject: RE: [Histonet] New Microwave Cap Regulation To: "'Rene J Buesa'" , "'Luck, Greg D.'" , "'Stacey Burton'" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" ANP.29430 Phase 1 This question doesn't state laboratory or home grade microwaves. Microwave should be placed in an appropriate ventilated hood to contain airborne chemical contaminates and potential infectious agents. Microwaves used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. This doesn't say microwave has to be laboratory grade, only the fume extractor. This checklist question doesn't apply if only non-hazardous reagents are used in the device (microwave) (e.g. water, certain biological stains) In my opinion you can use any microwave (laboratory or house microwave) as long as you can document: 1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface. 2. Periodically monitor for temperature reproducibility. 3. All containers used in the microwave are made from microwave-transparent material/ microwave proof. Don't make it any harder than it is. Hope this clears a few things, Hector -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 22, 2007 2:19 PM To: Luck, Greg D.; Stacey Burton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Microwave Cap Regulation Greg: The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab. To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment). Just my opinion though! Ren? J. "Luck, Greg D." wrote: Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 22 Feb 2007 17:03:08 -0600 From: "Joe Nocito" Subject: Re: [Histonet] PAS stain for nail fungus To: , Message-ID: <00c901c756d5$9e5609d0$649eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hey Doc, you have the South Texas Toenail lab guy right here. That's why I'm known as "Joe the Toe". We soak our nails in 20% ammonium hydroxide for at least an hour before we put the blocks on the machine to process. We've been getting nailed for a couple of years and perform a PAS for Fungus on each case that we do. Reimbursement must be good, we keep getting nailed. Joe the Toe or just JTT ----- Original Message ----- From: To: Sent: Thursday, February 22, 2007 12:09 PM Subject: [Histonet] PAS stain for nail fungus > Dr. Allen Smith's recent post reminds me of a question I've been meaning > to post to Histonet. > > Podiatrists are now performing biopsies of toenails with nail fungus, in > order to get a fungal stain (PAS is being requested, I think) done. > Apparently the insurance companies are now requiring a biopsy or culture > diagnosis of fungal disease before they'll pay for the very expensive > systemic drugs for nail fungus like terbinafine (Lamisil). > > Is anyone on Histonet getting these requests? What protocols do you use to > cut and stain these difficult specimens? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and security > tools, free access to millions of high-quality videos from across the web, > free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 22 Feb 2007 17:47:21 -0600 From: Marilyn McDonald Subject: [Histonet] LIS programs To: histonet@lists.utsouthwestern.edu Message-ID: <2E9C4E57-F430-4180-AF78-43EE869A89B5@mac.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks ------------------------------ Message: 18 Date: Thu, 22 Feb 2007 15:51:19 -0800 (PST) From: Akemi Allison-Tacha Subject: Re: [Histonet] PAS stain for nail fungus To: Joe Nocito , rsrichmond@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <86477.62101.qm@web31309.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Joe, I guess the histology field still uses the old methods. I was taught that back in 1974 at OHSU. FYI-We also used soak in NAIR HAIR REMOVAL to soften nails. Akemi Allison-Tacha --- Joe Nocito wrote: > Hey Doc, > you have the South Texas Toenail lab guy right here. > That's why I'm known as > "Joe the Toe". > We soak our nails in 20% ammonium hydroxide for > at least an hour before > we put the blocks on the machine to process. We've > been getting nailed for a > couple of years and perform a PAS for Fungus on each > case that we do. > Reimbursement must be good, we keep getting nailed. > > Joe the Toe or just JTT > > ----- Original Message ----- > From: > To: > Sent: Thursday, February 22, 2007 12:09 PM > Subject: [Histonet] PAS stain for nail fungus > > > > Dr. Allen Smith's recent post reminds me of a > question I've been meaning > > to post to Histonet. > > > > Podiatrists are now performing biopsies of > toenails with nail fungus, in > > order to get a fungal stain (PAS is being > requested, I think) done. > > Apparently the insurance companies are now > requiring a biopsy or culture > > diagnosis of fungal disease before they'll pay for > the very expensive > > systemic drugs for nail fungus like terbinafine > (Lamisil). > > > > Is anyone on Histonet getting these requests? What > protocols do you use to > > cut and stain these difficult specimens? > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > ________________________________________________________________________ > > Check out the new AOL. Most comprehensive set of > free safety and security > > tools, free access to millions of high-quality > videos from across the web, > > free AOL Mail and more. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 19 Date: Thu, 22 Feb 2007 15:56:11 -0800 From: "M C" Subject: [Histonet] I have an E300 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Is anyone in the market for an ASP-300 Tissue Processor. Contact: mc549@hotmail.com for more details. -Marco Corona _________________________________________________________________ Want a degree but can't afford to quit? Top school degrees online - in as fast as 1 year http://forms.nextag.com/goto.jsp?url=/serv/main/buyer/education.jsp?doSearch=n&tm=y&search=education_text_links_88_h288c&s=4079&p=5116 ------------------------------ Message: 20 Date: Thu, 22 Feb 2007 18:35:41 -0600 From: "Bonnie Whitaker" Subject: RE: [Histonet] LIS programs To: "'Marilyn McDonald'" , Message-ID: <000e01c756e2$8c8df660$3601a8c0@brownpathology.net> Content-Type: text/plain; charset="us-ascii" We LOVE WinSurge!! (If you are asking for Anatomic Path only). Feel free to contact me with any particular questions. Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 5:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 22 Feb 2007 18:39:54 -0600 From: "Joe Nocito" Subject: Re: [Histonet] PAS stain for nail fungus To: "Akemi Allison-Tacha" , , Message-ID: <001001c756e3$23859370$649eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original we tried Nair, but we are getting results with the 20% NH3OH JTT ----- Original Message ----- From: "Akemi Allison-Tacha" To: "Joe Nocito" ; ; Sent: Thursday, February 22, 2007 5:51 PM Subject: Re: [Histonet] PAS stain for nail fungus > Hi Joe, > > I guess the histology field still uses the old > methods. I was taught that back in 1974 at OHSU. > FYI-We also used soak in NAIR HAIR REMOVAL to soften > nails. > Akemi Allison-Tacha > > > --- Joe Nocito wrote: > >> Hey Doc, >> you have the South Texas Toenail lab guy right here. >> That's why I'm known as >> "Joe the Toe". >> We soak our nails in 20% ammonium hydroxide for >> at least an hour before >> we put the blocks on the machine to process. We've >> been getting nailed for a >> couple of years and perform a PAS for Fungus on each >> case that we do. >> Reimbursement must be good, we keep getting nailed. >> >> Joe the Toe or just JTT >> >> ----- Original Message ----- >> From: >> To: >> Sent: Thursday, February 22, 2007 12:09 PM >> Subject: [Histonet] PAS stain for nail fungus >> >> >> > Dr. Allen Smith's recent post reminds me of a >> question I've been meaning >> > to post to Histonet. >> > >> > Podiatrists are now performing biopsies of >> toenails with nail fungus, in >> > order to get a fungal stain (PAS is being >> requested, I think) done. >> > Apparently the insurance companies are now >> requiring a biopsy or culture >> > diagnosis of fungal disease before they'll pay for >> the very expensive >> > systemic drugs for nail fungus like terbinafine >> (Lamisil). >> > >> > Is anyone on Histonet getting these requests? What >> protocols do you use to >> > cut and stain these difficult specimens? >> > >> > Bob Richmond >> > Samurai Pathologist >> > Knoxville TN >> > >> > ________________________________________________________________________ >> > Check out the new AOL. Most comprehensive set of >> free safety and security >> > tools, free access to millions of high-quality >> videos from across the web, >> > free AOL Mail and more. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ------------------------------ Message: 22 Date: Fri, 23 Feb 2007 05:59:51 -0500 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] FDA approved recyclers To: "'Rene J Buesa'" , "'Mrosla, Tina M'" , Message-ID: <000701c75739$bea957f0$86f62d4b@HPPav2> Content-Type: text/plain; charset="iso-8859-1" Going to put in my 2 cents, which may not be too accurate. The FDA (Food and Drug Administration) covers topics that relate to Food and Drugs and medical devices that go INTO a HUMAN BEING, and anything else that touches these foods and drugs and medical devices that could effect a person's health. So as far as "recycling" would go, the FDA would be concerned about food or drugs that were being gathered after NOT being used and "recycled" for reuse (think of drugs after expiration date, or that had already been given to a patient and returned back to pharmacy). The FDA is also concerned about the plastic that goes around the food and drugs, as I remember they were concerned about new "recycled" plastic that the food and drugs are packaged in, and whether recycled plastic emit anything that would compromise the food or drugs and make it a health hazard. I know the FDA was concerned about medical devices (artificial hips, pace makers, etc.) being removed from people, cleaned up, and "recycled" for use in another person. As for a xylene/alcohol/formalin recylcer - I personally think this is out of the jurisdication of the FDA. The equipment itself does not go inside a person, nor is the intended use of these chemicals that are being recycled intended to human consumption. Neither the recycler nor the chemicals are intended for human USE. On the other hand, - OSHA has lots laws about chemical exposure to people that would apply - EPA would be concerned if the recycled chemicals got into the atmosphere or the water, and care about the proper disposal of waste - CAP has checklist questions for safety and use of equipment, electricity, chemicals, disposal, etc. that would apply. - I've never read the GLP, put I'm certain they have similar rules about chemicals and equipment. - Local fire marshals and the hospital's insurance company may have their own concern, especially as to where the recycler is positioned - but this is for scenarios of catching on fire and explosions. If someone knows of the FDA's concern in recycling chemicals in the laboratory, please inform HistoNet. Thanks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 22, 2007 12:50 PM To: Mrosla, Tina M; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FDA approved recyclers Tina: I also used a B/R recycler for more than 10 years, we were inspected frequently in our hospital and we were never told of a FDA requirement. If that information cost you any money, ask for your money back! Ren? J. "Mrosla, Tina M" wrote: We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval? Tina Mrosla, HTL (ASCP) Histotechnologist St. Joseph's Hospital Healtheast Care System 651-232-3575 fax 232-4543 tmmrosla@healtheast.org Passion for Caring and Service The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Fri, 23 Feb 2007 06:24:22 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] LIS programs To: "Marilyn McDonald" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D34F6@sjhaexc02.sjha.org> Content-Type: text/plain; charset="us-ascii" Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP programs. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 35 **************************************** From TJJ <@t> Stowers-Institute.org Fri Feb 23 08:14:57 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Feb 23 08:15:37 2007 Subject: [Histonet] RE: Dulbecco's PBS References: Message-ID: D. Garcia, You asked: 1) Does it matter whether one uses PBS with or without Mg and Ca for immunos? I have always done it with PBS without Mg and Ca, and have never heard of anyone doing it with Mg and Ca, but have recently encountered a scenario in which this was the only PBS available. Wondering whether this will make a difference in my staining. I don't know, I've never used Dulbecco's PBS. Gayle Callis uses this, perhaps she can address this point. 2) Can anyone explain the need for dehydrating and defatting slides (after using such substrates as DAB for color development) before coverslipping? Aside from the obvious that the sections look pretty crappy without these steps, can anyone explain the science behind this? I don't think there is really anything scientific about this practice. At this point we are not using alcohol and xylene to defat the slides. I think it's more that after counterstaining, the slides are in water and water is incompatible with xylene or toluene based mounting media. Therefore, we dehydrate into graded alcohols. Xylene is used as the intermediary step when the mountant is incompatible with alcohol, and the slides are coverslipped wet from this reagent. Surgipath has an mounting medium, Clearium, that you can coverslip from alcohol. While it is possible to air dry the slides after the water rinses after hematoxylin (or other) counterstain, and dry coverslip them using permanent mounting medium, we routinely dehydrate, clear and mount to coverslip H&Es and other histochemical stains. Why should we treat the immunostained slides differently? (Unless, of course, the chromogen is soluble in organic solvents.) It is possible there are imaging issues that might crop up by coverslipping air-dried slides, but I'm personally not aware of any. 3) Because my tissue is perfused, I typically store the brains in 30% sucrose (with a little bit of sodium azide) in 4 degrees, until I am ready to cut the blocks. At this point, brains are usually frozen in the cryostat just before cutting 40um thick sections. I have obtained wonderful staining and morphology with this technique, however I have been recently advised to store the brains embedded in OCT (or similar) in a -20 degree =66reezer, rather than in 4 degrees. I am skeptical and concerned that this will damage the morphology and produce that "swiss cheese" freezer artifact (you know, where there are all these holes in the tissue?) Can anyone provide any advice, insight as to the benefits and drawbacks to either/both of these methods? If you snap-freeze your samples and store them properly in a -20 (or -80) degree C freezer that is not self-defrosting, your samples will be fine. The freezer artifact would come from improper snap freezing, or repeated freeze/thaw cycles upon storage. I have found that samples stored long term in -80 may be more difficult to section. While it doesn't damage the sample, it does change it's sectioning characteristics as compared to samples stored at -20. It would be my preference to store them long term frozen in OCT than in an aqueous solution. Although your tissue is perfusion fixed, it may still be possible to reverse the aldehyde cross-links with long term storage in aqueous solutions. I don't know for sure if the sucrose blocks that possibility or eventuality, and because I don't know that, I'd rather be safe than introduce another variable into a research hypothesis. Do you know for sure there is no difference if you store Sample A for 2 weeks in sucrose in the fridge, and when you repeat the experiment with Sample B (which was harvested at the same time as sample A), it has been stored in the fridge for an addition week (or two or three), etc.? How long do you store them in the refrigerator? You don't mention this. I would worry about solubility of certain antigens or proteins as time in storage was increased. Again, I have no evidence to suggest it happens, as proving that for particular antigens or proteins is a research experiment in itself. Our responsibility to the researchers here is to preserve the integrity of their sample and not introduce variables from sample to sample as much as possible. The best way to accomplish that is to treat all samples the same, and our practice is to freeze them after storage in 30% sucrose after 24 hours. My first thought about storage in 30% sucrose was microbial growth, but I see you use sodium azide in your solution. Have you seen any evidence sodium azide remains in your sample, possibly inhibiting HRP in your subsequent immunostaining? Best wishes, Teri Johnson Stowers Institute for Medical Research Kansas City, MO 64110 From LGaliotto <@t> nch.org Fri Feb 23 08:35:58 2007 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Fri Feb 23 08:36:15 2007 Subject: [Histonet] Pneumocystis controls Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B16327@NCH01EX02.nch.org> Hello and Happy Friday, Can anyone suggest a vendor that supplies Pneumocystis (smear) controls? One that does not have problems with supply demand. If so please forward me the company name. Thank you Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From alaskagirl1950 <@t> yahoo.com Fri Feb 23 08:55:13 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Feb 23 08:55:28 2007 Subject: [Histonet] Bone Necrosis Message-ID: <605000.29042.qm@web52503.mail.yahoo.com> Hello everyone, It is finally Friday! My second Friday this week, since I had myself convinced that yesterday was also Friday....bummer! Now my question to everyone! A dog came into the Vet School Clinic and had a bone biopsy on the right front leg. My Pathologist says that it looks like bone necrosis or osteoporosis. Is there a stain that I can do to show bone necrosis? In looking at the X-rays a bullet was found, and she is wondering if it could be necrosis because of the bullet instead of osteoporosis. Does anyone have suggestions? Thank you in advance, Patricia Adams, HT (ASCP) Tuskegee University School of Veterinary Medicine ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 From rjbuesa <@t> yahoo.com Fri Feb 23 09:04:59 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 23 09:05:07 2007 Subject: [Histonet] Bone Necrosis In-Reply-To: <605000.29042.qm@web52503.mail.yahoo.com> Message-ID: <753138.15813.qm@web61217.mail.yahoo.com> Patricia: Excuse my ignorance but, as far as I know, necrosis is one thing and osteoporosis is another. Necrosis is decayed bone due to a dead structure and osteoporosis is the loss of bone due to decalcification usually an aging process. Perhaps you could clarify me that. About the stain: any stain for bone (or even a simple H&E) will stain the structures, it is then that the pathologist, based in what she sees, to stablish the diagnosis. Also if what she sees in the X-rays is localized and near the bullet, that should be the cause. Osteoporosis is a more generalized process that will be seen even far from the bullet. Just my 2 cents! Ren? J. Patricia Adams wrote: Hello everyone, It is finally Friday! My second Friday this week, since I had myself convinced that yesterday was also Friday....bummer! Now my question to everyone! A dog came into the Vet School Clinic and had a bone biopsy on the right front leg. My Pathologist says that it looks like bone necrosis or osteoporosis. Is there a stain that I can do to show bone necrosis? In looking at the X-rays a bullet was found, and she is wondering if it could be necrosis because of the bullet instead of osteoporosis. Does anyone have suggestions? Thank you in advance, Patricia Adams, HT (ASCP) Tuskegee University School of Veterinary Medicine ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. From Barry.R.Rittman <@t> uth.tmc.edu Fri Feb 23 09:19:31 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Feb 23 09:19:36 2007 Subject: [Histonet] Bone Necrosis In-Reply-To: <753138.15813.qm@web61217.mail.yahoo.com> Message-ID: I am not a pathologist but if there is osteoporosis I believe that this would generally be in more than one site and may be shown with X-rays. Necrosis on the other hand, I would assume in this case, to be more localized and due to the local injury. (Necrosis is also classified as various types). A general histological examination of the area, presence of cell debris, apoptotic cells, inflammatory cells and their types, etc. should allow you to distinguish between necrosis and osteoporosis. Let's have comments from pathologists dealing with bone please. A small point is that here in Texas (and I'm sure that Rene would agree) we like to be specific, was the bullet a 38, 44, 45, 9mm etc? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 23, 2007 9:05 AM To: Patricia Adams; HistoNet Subject: Re: [Histonet] Bone Necrosis Patricia: Excuse my ignorance but, as far as I know, necrosis is one thing and osteoporosis is another. Necrosis is decayed bone due to a dead structure and osteoporosis is the loss of bone due to decalcification usually an aging process. Perhaps you could clarify me that. About the stain: any stain for bone (or even a simple H&E) will stain the structures, it is then that the pathologist, based in what she sees, to stablish the diagnosis. Also if what she sees in the X-rays is localized and near the bullet, that should be the cause. Osteoporosis is a more generalized process that will be seen even far from the bullet. Just my 2 cents! Ren? J. Patricia Adams wrote: Hello everyone, It is finally Friday! My second Friday this week, since I had myself convinced that yesterday was also Friday....bummer! Now my question to everyone! A dog came into the Vet School Clinic and had a bone biopsy on the right front leg. My Pathologist says that it looks like bone necrosis or osteoporosis. Is there a stain that I can do to show bone necrosis? In looking at the X-rays a bullet was found, and she is wondering if it could be necrosis because of the bullet instead of osteoporosis. Does anyone have suggestions? Thank you in advance, Patricia Adams, HT (ASCP) Tuskegee University School of Veterinary Medicine ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peak at the forecast with theYahoo! Search weather shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Fri Feb 23 09:22:54 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Feb 23 09:23:06 2007 Subject: [Histonet] tampa employment opportunity Message-ID: <8C925759FE27B3A-1220-294E@WEBMAIL-RB17.sysops.aol.com> We are rapidly growing and are seeking candidates for the following positions: Histotechs Cytotechs Lab Aides Cyto Prep techs Data Entry Clerks Couriers If you or anyone you know are interested, please reply directly to me...... Thank you Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From staceylburton <@t> yahoo.com Fri Feb 23 10:05:10 2007 From: staceylburton <@t> yahoo.com (Stacey Burton) Date: Fri Feb 23 10:05:20 2007 Subject: [Histonet] New Microwave Cap Regulation Message-ID: <878938.82563.qm@web30007.mail.mud.yahoo.com> Thank you Hector. This brings me to a new question - How do I test for leakage is less than 5mW/cm2 at a distance of 5 cm from the surface? Or what type of service to I call to help me with that? -Stacey ----- Original Message ---- From: Hector Hernandez To: Rene J Buesa ; "Luck, Greg D." ; Stacey Burton ; histonet@lists.utsouthwestern.edu Sent: Thursday, February 22, 2007 4:19:54 PM Subject: RE: [Histonet] New Microwave Cap Regulation ANP.29430 Phase 1 This question doesn't state laboratory or home grade microwaves. Microwave should be placed in an appropriate ventilated hood to contain airborne chemical contaminates and potential infectious agents. Microwaves used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. This doesn't say microwave has to be laboratory grade, only the fume extractor. This checklist question doesn't apply if only non-hazardous reagents are used in the device (microwave) (e.g. water, certain biological stains) In my opinion you can use any microwave (laboratory or house microwave) as long as you can document: 1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface. 2. Periodically monitor for temperature reproducibility. 3. All containers used in the microwave are made from microwave-transparent material/ microwave proof. Don't make it any harder than it is. Hope this clears a few things, Hector -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 22, 2007 2:19 PM To: Luck, Greg D.; Stacey Burton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Microwave Cap Regulation Greg: The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab. To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment). Just my opinion though! Ren? J. "Luck, Greg D." wrote: Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Don't get soaked. Take a quick peak at the forecast with the Yahoo! Search weather shortcut. http://tools.search.yahoo.com/shortcuts/#loc_weather From jmahoney <@t> alegent.org Fri Feb 23 10:05:47 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Feb 23 10:06:13 2007 Subject: [Histonet] LIS programs In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D34F6@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D34F6@sjhaexc02.sjha.org> Message-ID: <45DEBC7B0200003C00006365@gwia.alegent.org> We have Cerner millennium and I absolutely love it. If CoPath is the Mercedes Cerner is the Maserati, in my opinion. Please feel free to call me if you want to know details. Jan Omaha 402-717-2889 >>> "Weems, Joyce" 02/23/2007 5:24 AM >>> Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP programs. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Fri Feb 23 10:13:45 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Feb 23 10:13:55 2007 Subject: [Histonet] Bone Necrosis In-Reply-To: Message-ID: <625614.74511.qm@web52509.mail.yahoo.com> I agree that the cacaliberf the bullet is of importance, I grew up on a homestead in Alaska. Any time my siblings and I went walking, I had to carry my fathers 357 in a holster....quite a few years ago. Had to protect ourselves from moose and bear! Also didn't have elelectricitynd running water (did have indoor plumbing). I just spoke to the Pathologist and she said the bullet had been removed before, but seem that the bone reaction is close to that area. She had to teach a class, when I am able to sit and talk to her I will try to understand more what she is looking for. Thank you so much for the help so far. Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. http://games.yahoo.com/games/front From RohrT <@t> nyackhospital.org Fri Feb 23 10:14:34 2007 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Fri Feb 23 10:17:15 2007 Subject: [Histonet] Extended time in paraffin Message-ID: I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? I have only found two clear commentaries. Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. Can any of you offer me any further references and/or assistance or your own methods/experiences? Thank you so much for your time and assistance Theresa Rohr Nyack Hospital, NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From ian.montgomery <@t> bio.gla.ac.uk Fri Feb 23 10:20:50 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Feb 23 10:21:06 2007 Subject: [Histonet] Alkaline Phosphatase. Message-ID: <00a101c75766$95950300$4724d182@ibls.gla.ac.uk> Several weeks ago there was a request for an alkaline phosphatase technique that could be used on wax sections. My humble apologies but I overlooked replying. My method of choice is, McGadey,J. 1970. Histochemie. 23. 180-184. Tetrazolium method for non-specific Alkaline Phosphatase. I've used the technique on cryostat sections, fresh or lightly fixed in Baker's formol calcium, carefully processed paraffin sections and resin embedded sections. In all instances the method worked superbly. If you're interested and cannot get the journal let me know and I'll e-mail the technique. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. From JWEEMS <@t> sjha.org Fri Feb 23 10:21:11 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 23 10:21:47 2007 Subject: [Histonet] Extended time in paraffin Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D34FF@sjhaexc02.sjha.org> Number 2 would be the option I recommend. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Theresa Rohr Sent: Friday, February 23, 2007 11:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Extended time in paraffin I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? I have only found two clear commentaries. Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. Can any of you offer me any further references and/or assistance or your own methods/experiences? Thank you so much for your time and assistance Theresa Rohr Nyack Hospital, NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From MadaryJ <@t> MedImmune.com Fri Feb 23 10:33:13 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Feb 23 10:33:28 2007 Subject: [Histonet] recycling argument for Message-ID: <8F3E1865E343C943BB38506D56FF015157B5A4@MD1EV002.medimmune.com> Seems like a couple of folks are against recycling, but in reality it does save money, and reduce the stress we place on Earth. As far as exposure, it can be minimized if we use proper safety techniques. I saw someone said we buy formalin to reduce out exposure, and although I cannot argue with that, we use it because there are no substitutes out there that we feel are better than NBF. From LRaff <@t> lab.uropartners.com Fri Feb 23 10:33:52 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Fri Feb 23 10:34:01 2007 Subject: [Histonet] Extended time in paraffin Message-ID: <5DA1CA5D0B98A84985B545A24423B822052D5C@UPLAB01.uplab.local> We were not happy with our results (fragments were drying out) when tissue sat in formalin in our VIP processor over the weekend, so now we run our weekend cases (all small biopsies) on Friday afternoon, and then follow your option #2. It works well for us. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa Rohr Sent: Friday, February 23, 2007 10:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Extended time in paraffin I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? I have only found two clear commentaries. Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. Can any of you offer me any further references and/or assistance or your own methods/experiences? Thank you so much for your time and assistance Theresa Rohr Nyack Hospital, NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Feb 23 10:36:20 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 23 10:36:52 2007 Subject: [Histonet] LIS programs Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3504@sjhaexc02.sjha.org> Cerner bought CoPath, so maybe they combined the two to make the Maserati! -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Friday, February 23, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu; Marilyn McDonald; Weems, Joyce Subject: RE: [Histonet] LIS programs We have Cerner millennium and I absolutely love it. If CoPath is the Mercedes Cerner is the Maserati, in my opinion. Please feel free to call me if you want to know details. Jan Omaha 402-717-2889 >>> "Weems, Joyce" 02/23/2007 5:24 AM >>> Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP programs. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From BoozerKA <@t> ah.org Fri Feb 23 10:39:34 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Fri Feb 23 10:40:26 2007 Subject: [Histonet] PAS stain for nail fungus In-Reply-To: <8C924C3B79DE816-1784-31C9@MBLK-M22.sysops.aol.com> References: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> <8C924C3B79DE816-1784-31C9@MBLK-M22.sysops.aol.com> Message-ID: <45DEA845.4AA8.00C0.0@ah.org> Nair is placed on the nail when grossed by the pathology assistant for 1 hour and then cut. The histotechs put nair on it after it is faced for a couple of minutes and cut it on eith + or poly-l-Lysine slides. A PAS is done and it stays on great! >>> 02/22/2007 10:09 >>> Dr. Allen Smith's recent post reminds me of a question I've been meaning to post to Histonet. Podiatrists are now performing biopsies of toenails with nail fungus, in order to get a fungal stain (PAS is being requested, I think) done. Apparently the insurance companies are now requiring a biopsy or culture diagnosis of fungal disease before they'll pay for the very expensive systemic drugs for nail fungus like terbinafine (Lamisil). Is anyone on Histonet getting these requests? What protocols do you use to cut and stain these difficult specimens? Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From valleygal <@t> aol.com Fri Feb 23 10:42:28 2007 From: valleygal <@t> aol.com (valleygal@aol.com) Date: Fri Feb 23 10:42:55 2007 Subject: [Histonet] Extended time in paraffin In-Reply-To: References: Message-ID: <8C92580BD6C25F1-10D0-581E@mblk-r13.sysops.aol.com> Can you select the number of the station that you want to delay the tissues in until processing starts? If you can, you could have the tissues go through the formalins like usual and delay when they get to the first alcohol station. The processor should then start up and continue on with the processing in time for the tissues to be done early Mon AM. Otherwise, either option 2 or 3 would work. 2 would take less time to remelt. Andi Grantham -----Original Message----- From: RohrT@nyackhospital.org To: Histonet@lists.utsouthwestern.edu Sent: Fri, 23 Feb 2007 9:14 AM Subject: [Histonet] Extended time in paraffin I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? I have only found two clear commentaries. Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. Can any of you offer me any further references and/or assistance or your own methods/experiences? Thank you so much for your time and assistance Theresa Rohr Nyack Hospital, NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From godsgalnow <@t> aol.com Fri Feb 23 10:51:05 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Feb 23 10:51:21 2007 Subject: [Histonet] Extended time in paraffin In-Reply-To: References: Message-ID: <8C92581F1B5F83F-1220-33FB@WEBMAIL-RB17.sysops.aol.com> I vore for option number 2. Roxanne -----Original Message----- From: RohrT@nyackhospital.org To: Histonet@lists.utsouthwestern.edu Sent: Fri, 23 Feb 2007 11:14 AM Subject: [Histonet] Extended time in paraffin I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? I have only found two clear commentaries. Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. Can any of you offer me any further references and/or assistance or your own methods/experiences? Thank you so much for your time and assistance Theresa Rohr Nyack Hospital, NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From akemiat3377 <@t> yahoo.com Fri Feb 23 10:53:36 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Feb 23 10:58:06 2007 Subject: [Histonet] PAS stain for nail fungus In-Reply-To: <45DEA845.4AA8.00C0.0@ah.org> References: <200702221122.60a45ddc35333c@rly-xm04.mx.aol.com> <8C924C3B79DE816-1784-31C9@MBLK-M22.sysops.aol.com> <45DEA845.4AA8.00C0.0@ah.org> Message-ID: <1EC6D9C6-12AC-48F7-BF20-4088278BD968@yahoo.com> Hi Kathy, Since you too are from Portland, OR, that technique was paced down to most of the histotechs in OR years ago. If I can move the cobwebs from my brain around, Lee Luna gave us that tip when he came out to give a talk in the early 80's. Are you still at Portland Adventist? Akemi Phoenix Lab Consulting Specializing in Histology, SS, IHC, & Microarray Madison, WI Akemi Allison-Tacha BS, HT (ASCP) HTL President Cell: (925) 788-0900 E-Mail: akemiat3377@yahoo.com On Feb 23, 2007, at 10:39 AM, Kathleen Boozer wrote: > Nair is placed on the nail when grossed by the pathology assistant > for 1 hour and then cut. The histotechs put nair on it after it is > faced for a couple of minutes and cut it on eith + or poly-l-Lysine > slides. A PAS is done and it stays on great! > >>>> 02/22/2007 10:09 >>> > Dr. Allen Smith's recent post reminds me of a question I've been > meaning to post to Histonet. > > Podiatrists are now performing biopsies of toenails with nail > fungus, in order to get a fungal stain (PAS is being requested, I > think) done. Apparently the insurance companies are now requiring a > biopsy or culture diagnosis of fungal disease before they'll pay > for the very expensive systemic drugs for nail fungus like > terbinafine (Lamisil). > > Is anyone on Histonet getting these requests? What protocols do you > use to cut and stain these difficult specimens? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ______________________________________________________________________ > __ > Check out the new AOL. Most comprehensive set of free safety and > security tools, free access to millions of high-quality videos from > across the web, free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Fri Feb 23 10:59:40 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Feb 23 11:00:05 2007 Subject: [Histonet] recycling argument for In-Reply-To: <8F3E1865E343C943BB38506D56FF015157B5A4@MD1EV002.medimmune.com> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A534@SCREECH.ntcampus.smdc.org> I agree with you Joseph. We may have to take safety precautions with formalin. But, at least we know that. I worry about other reagents (new formalin substitutes) only because we don't and won't know the effects until some time has gone by. Formalin is easy to neutralize as well. It's like many other things that must be handled properly. I understand the idea about limiting exposure by not recycling, but there is a down side as well. Somebody (environmental services personnel, e.g.)has to handle the formalin you are not recycling, so you've only shifted the exposure. If you have your financial people (beancounters) perform a cost analysis recyclers generally end up paying for themselves over a set period of time. This is doubly green, in both an environmental and fiscal sense. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, Joseph Sent: Friday, February 23, 2007 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycling argument for Seems like a couple of folks are against recycling, but in reality it does save money, and reduce the stress we place on Earth. As far as exposure, it can be minimized if we use proper safety techniques. I saw someone said we buy formalin to reduce out exposure, and although I cannot argue with that, we use it because there are no substitutes out there that we feel are better than NBF. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From sbreeden <@t> nmda.nmsu.edu Fri Feb 23 11:15:43 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Feb 23 11:15:53 2007 Subject: [Histonet] "Joe The Toe" Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB492@nmdamailsvr.nmda.ad.nmsu.edu> Well, that clarifies THAT! And what a relief, as I had other reasons for that nickname in MY mind! TGIF and then some! Anybody need to Fume? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From funderwood <@t> mcohio.org Fri Feb 23 11:28:49 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Feb 23 11:31:02 2007 Subject: [Histonet] "Joe The Toe" Message-ID: I thought maybe Joe kicked for the 1963 Cleveland Browns. >>> "Breeden, Sara" 2/23/2007 12:15 PM >>> Well, that clarifies THAT! And what a relief, as I had other reasons for that nickname in MY mind! TGIF and then some! Anybody need to Fume? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Feb 23 11:32:31 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Feb 23 11:32:51 2007 Subject: [Histonet] "Joe The Toe" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB492@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB492@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <45DED0D00200003C00006391@gwia.alegent.org> Uh Oh...here we go, it's Friday. Time to digress. I'm ready for a glass of wine and its only 11:30am in Omaha. Jan >>> "Breeden, Sara" 02/23/2007 11:15 AM >>> Well, that clarifies THAT! And what a relief, as I had other reasons for that nickname in MY mind! TGIF and then some! Anybody need to Fume? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Fri Feb 23 11:37:05 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Feb 23 11:37:14 2007 Subject: [Histonet] recycling argument for Message-ID: All, Speaking of formalin...did anyone go through the new HER2 guidlines for breast. They just re-enforce my belief that it would serve ALL of us best if we could stay with formalin as our fixative and concentrate on things like recycling it rather than on formalin substitutes. Fixation in anything other than formalin can create issues with so many tests down the line. If we all used formalin, we could freely send our blocks to each other with less worry from testing variations due to differing fixation. Just My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jasper, Thomas G. Sent: Friday, February 23, 2007 11:00 AM To: Madary, Joseph Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recycling argument for I agree with you Joseph. We may have to take safety precautions with formalin. But, at least we know that. I worry about other reagents (new formalin substitutes) only because we don't and won't know the effects until some time has gone by. Formalin is easy to neutralize as well. It's like many other things that must be handled properly. I understand the idea about limiting exposure by not recycling, but there is a down side as well. Somebody (environmental services personnel, e.g.)has to handle the formalin you are not recycling, so you've only shifted the exposure. If you have your financial people (beancounters) perform a cost analysis recyclers generally end up paying for themselves over a set period of time. This is doubly green, in both an environmental and fiscal sense. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, Joseph Sent: Friday, February 23, 2007 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycling argument for Seems like a couple of folks are against recycling, but in reality it does save money, and reduce the stress we place on Earth. As far as exposure, it can be minimized if we use proper safety techniques. I saw someone said we buy formalin to reduce out exposure, and although I cannot argue with that, we use it because there are no substitutes out there that we feel are better than NBF. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Feb 23 11:38:22 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Feb 23 11:38:45 2007 Subject: Fwd: RE: [Histonet] LIS programs References: Message-ID: <45DED22E0200003C00006397@gwia.alegent.org> I was on our hospital system's LIS build team. PathNet is a very deep system with many capabilities. It can be built to serve your needs. What I like is that flexibility. To each his own, but if you have somebody who knows what you want in a LIS for anatomic pathology (histo tech) actually building the system or at the very least very directly involved, it is a very user friendly system with endless capabilities. We looked at Copath when we were choosing a system. CoPath is very good and i know a lot of people like it but I'm glad we decided on PathNet. Jan Omaha >>> "Chaussey, Leslie" 02/23/2007 10:51 AM >>> Actually, they're distinct systems. I've worked with both MiSys CoPath and Cerner PathNet. I generally describe PathNet as the Kia of the AP LIS world. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, February 23, 2007 10:36 AM To: Janice Mahoney; histonet@lists.utsouthwestern.edu; Marilyn McDonald Subject: RE: [Histonet] LIS programs Cerner bought CoPath, so maybe they combined the two to make the Maserati! -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Friday, February 23, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu; Marilyn McDonald; Weems, Joyce Subject: RE: [Histonet] LIS programs We have Cerner millennium and I absolutely love it. If CoPath is the Mercedes Cerner is the Maserati, in my opinion. Please feel free to call me if you want to know details. Jan Omaha 402-717-2889 >>> "Weems, Joyce" 02/23/2007 5:24 AM >>> Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP programs. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From MMargiotta <@t> bmhmc.org Fri Feb 23 11:39:51 2007 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Fri Feb 23 11:39:59 2007 Subject: [Histonet] Formalin waste Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF5F4@bmh_exchange.bmhmc.org> Hi All, Just wondering how most labs deal with disposing of their tissue specimens that have been fixed in formalin. We have to pour off the formalin to separate the tissue so that it can be disposed of in red bag garbage. This is such a time consuming and dirty job. Adequate ventilation is also a problem. Does anyone out there have a different method? Thanks, Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From jayant_limaye <@t> yahoo.com Fri Feb 23 11:44:37 2007 From: jayant_limaye <@t> yahoo.com (jayant limaye) Date: Fri Feb 23 11:44:48 2007 Subject: Fwd: RE: [Histonet] LIS programs In-Reply-To: <45DED22E0200003C00006397@gwia.alegent.org> Message-ID: <855744.76891.qm@web51311.mail.yahoo.com> Hi Marilyn, Janice, i run a small group out of Irvine California with development work done out of India. Was with Quest Diagnostics before starting this group 2 years back. If you or any of the fellow Histonet members need to build a customized LIS system, we would be glad to help. You can call me at (949)-273-0405. Regards, Jayant Gencache http://www.gencache.com --- Janice Mahoney wrote: > > I was on our hospital system's LIS build team. > PathNet is a very deep > system with many capabilities. It can be built to > serve your needs. > What I like is that flexibility. To each his own, > but if you have > somebody who knows what you want in a LIS for > anatomic pathology > (histo tech) actually building the system or at the > very least very > directly involved, it is a very user friendly system > with endless > capabilities. > We looked at Copath when we were choosing a system. > CoPath is very > good and i know a lot of people like it but I'm glad > we decided on > PathNet. > Jan > Omaha > > >>> "Chaussey, Leslie" 02/23/2007 > 10:51 AM >>> > Actually, they're distinct systems. I've worked > with both MiSys > CoPath > and Cerner PathNet. I generally describe PathNet as > the Kia of the AP > LIS world. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Weems, > Joyce > Sent: Friday, February 23, 2007 10:36 AM > To: Janice Mahoney; > histonet@lists.utsouthwestern.edu; Marilyn > McDonald > Subject: RE: [Histonet] LIS programs > > Cerner bought CoPath, so maybe they combined the two > to make the > Maserati! > > -----Original Message----- > From: Janice Mahoney [mailto:jmahoney@alegent.org] > Sent: Friday, February 23, 2007 11:06 AM > To: histonet@lists.utsouthwestern.edu; Marilyn > McDonald; Weems, Joyce > Subject: RE: [Histonet] LIS programs > > > We have Cerner millennium and I absolutely love it. > If CoPath is the > Mercedes Cerner is the Maserati, in my opinion. > Please feel free to call me if you want to know > details. > Jan > Omaha > 402-717-2889 > > >>> "Weems, Joyce" 02/23/2007 5:24 > AM >>> > Triple G is NOT a good system. I hear that CoPath is > the Mercedes of > AP > programs. > > Good luck! > > Joyce > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital of Atlanta > 404-851-7376 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of > Marilyn > McDonald > Sent: Thursday, February 22, 2007 6:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] LIS programs > > What is your favorite Pathology program? What do you > like about it? > I am familiar with Tamtron (Powerpath). A friend > has asked me so I > decided to post it on the Histonet. No vendors > please. > Thanks > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained > in this message > may > be privileged and is confidential information > intended for the use of > the addressee listed above. If you are neither the > intended recipient > nor the employee or agent responsible for delivering > this message to > the > intended recipient, you are hereby notified that any > disclosure, > copying, distribution or the taking of any action in > reliance on the > contents of this information is strictly prohibited. > If you have > received this communication in error, please notify > us immediately by > replying to the message and deleting it from your > computer. Thank you. > Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Confidentiality Notice ** The information contained > in this message > may > be privileged and is confidential information > intended for the use of > the addressee listed above. If you are neither the > intended recipient > nor the employee or agent responsible for delivering > this message to > the > intended recipient, you are hereby notified that any > disclosure, > copying, distribution or the taking of any action in > reliance on the > contents of this information is strictly prohibited. > If you have > received this communication in error, please notify > us immediately by > replying to the message and deleting it from your > computer. Thank you. > Saint Joseph's Health System, Inc. > > ----------------------------------------- > This message and any included attachments are > intended only for the > addressee. The information contained in this message > is > confidential and may constitute proprietary or > non-public > information under international, federal, or state > laws. > Unauthorized forwarding, printing, copying, > distribution, or use of > such information is strictly prohibited and may be > unlawful. If you > are not the addressee, please promptly delete this > message and > notify the sender of the delivery error by e-mail. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barry.R.Rittman <@t> uth.tmc.edu Fri Feb 23 11:45:08 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Feb 23 11:45:17 2007 Subject: [Histonet] Formalin waste In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF5F4@bmh_exchange.bmhmc.org> Message-ID: In our institute the Hazardous waste people deal with this. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Friday, February 23, 2007 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin waste Hi All, Just wondering how most labs deal with disposing of their tissue specimens that have been fixed in formalin. We have to pour off the formalin to separate the tissue so that it can be disposed of in red bag garbage. This is such a time consuming and dirty job. Adequate ventilation is also a problem. Does anyone out there have a different method? Thanks, Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Fri Feb 23 11:46:42 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Fri Feb 23 11:46:51 2007 Subject: [Histonet] Lab Vision Bond system for Immunostaining Message-ID: <9E6D52F532809247BDA1783680E92C560B70279E@CHWEXC.chwi.chswi.org> I am in the process of evaluating immuno stainers and would like input from users of the Bond system as well as other stainers. Thank you, Annette Foshey, HT Histology Lab Children's Hospital of Wisconsin ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From bhewlett <@t> cogeco.ca Fri Feb 23 11:47:53 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Feb 23 11:48:01 2007 Subject: [Histonet] recycling argument for References: Message-ID: <006e01c75772$bef89980$6500a8c0@mainbox> Glen, I agree!! That would just leave the duration of fixation in NBF as the major issue! Bryan ----- Original Message ----- From: "Dawson, Glen" Cc: Sent: Friday, February 23, 2007 12:37 PM Subject: RE: [Histonet] recycling argument for All, Speaking of formalin...did anyone go through the new HER2 guidlines for breast. They just re-enforce my belief that it would serve ALL of us best if we could stay with formalin as our fixative and concentrate on things like recycling it rather than on formalin substitutes. Fixation in anything other than formalin can create issues with so many tests down the line. If we all used formalin, we could freely send our blocks to each other with less worry from testing variations due to differing fixation. Just My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jasper, Thomas G. Sent: Friday, February 23, 2007 11:00 AM To: Madary, Joseph Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recycling argument for I agree with you Joseph. We may have to take safety precautions with formalin. But, at least we know that. I worry about other reagents (new formalin substitutes) only because we don't and won't know the effects until some time has gone by. Formalin is easy to neutralize as well. It's like many other things that must be handled properly. I understand the idea about limiting exposure by not recycling, but there is a down side as well. Somebody (environmental services personnel, e.g.)has to handle the formalin you are not recycling, so you've only shifted the exposure. If you have your financial people (beancounters) perform a cost analysis recyclers generally end up paying for themselves over a set period of time. This is doubly green, in both an environmental and fiscal sense. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, Joseph Sent: Friday, February 23, 2007 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycling argument for Seems like a couple of folks are against recycling, but in reality it does save money, and reduce the stress we place on Earth. As far as exposure, it can be minimized if we use proper safety techniques. I saw someone said we buy formalin to reduce out exposure, and although I cannot argue with that, we use it because there are no substitutes out there that we feel are better than NBF. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Feb 23 11:48:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 23 11:48:33 2007 Subject: [Histonet] Formalin waste Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3507@sjhaexc02.sjha.org> We pour off the larger specimens, but dump smaller containers, formalin and all into double red bags sprinkled with Neutralex. No problems so far. (That I know of!) j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margiotta, Michele Sent: Friday, February 23, 2007 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin waste Hi All, Just wondering how most labs deal with disposing of their tissue specimens that have been fixed in formalin. We have to pour off the formalin to separate the tissue so that it can be disposed of in red bag garbage. This is such a time consuming and dirty job. Adequate ventilation is also a problem. Does anyone out there have a different method? Thanks, Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Lance.Erickson <@t> intermountainmail.org Fri Feb 23 11:58:19 2007 From: Lance.Erickson <@t> intermountainmail.org (Lance Erickson) Date: Fri Feb 23 11:58:50 2007 Subject: [Histonet] New Microwave Cap Regulation Message-ID: <8B08EC394B366D4A895DD5686D6AFE4A57FFE6@LP-EXCHVS08.CO.IHC.COM> Be careful and check the new CAP checklist that went into effect December of 2006. ANP 29430 about the venting is not the question that addresses the lab vs. home grade microwaves. The new checklist question is as follows: **NEW** 12/12/2006 ANP.27170 Phase I N/A YES NO Are microwave devices used in accordance with manufacturer's instructions? NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. And Stacey the question about microwave leakage ANP 27720 for measuring the devices annually to ensure the 5mW/cm2 leakage from the surface has been deleted. The leakage has been incorporated into ANP 28290 that is as follows: **NEW** 10/06/2005 **REVISED** 12/12/2006 ANP.28290 Phase I N/A YES NO Are microwave devices periodically monitored for reproducibility? NOTE: "Reproducibility" is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist question (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. It is a good idea to go to the CAP website and print off the changes to the AP checklist. There is also changes to ANP 28860 about containers in the microwave. Hope this info is helpful. Lance Erickson, PA, HTL (ASCP) Primary Children's Medical Center Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Friday, February 23, 2007 9:05 AM To: Hector Hernandez; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New Microwave Cap Regulation Thank you Hector. This brings me to a new question - How do I test for leakage is less than 5mW/cm2 at a distance of 5 cm from the surface? Or what type of service to I call to help me with that? -Stacey ----- Original Message ---- From: Hector Hernandez To: Rene J Buesa ; "Luck, Greg D." ; Stacey Burton ; histonet@lists.utsouthwestern.edu Sent: Thursday, February 22, 2007 4:19:54 PM Subject: RE: [Histonet] New Microwave Cap Regulation ANP.29430 Phase 1 This question doesn't state laboratory or home grade microwaves. Microwave should be placed in an appropriate ventilated hood to contain airborne chemical contaminates and potential infectious agents. Microwaves used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. This doesn't say microwave has to be laboratory grade, only the fume extractor. This checklist question doesn't apply if only non-hazardous reagents are used in the device (microwave) (e.g. water, certain biological stains) In my opinion you can use any microwave (laboratory or house microwave) as long as you can document: 1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface. 2. Periodically monitor for temperature reproducibility. 3. All containers used in the microwave are made from microwave-transparent material/ microwave proof. Don't make it any harder than it is. Hope this clears a few things, Hector -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 22, 2007 2:19 PM To: Luck, Greg D.; Stacey Burton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Microwave Cap Regulation Greg: The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab. To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment). Just my opinion though! Ren? J. "Luck, Greg D." wrote: Hello all, Would venting or use of a "Laboratory Grade" microwave be required if all that it is used for is to heat water or PBS or Tris buffers? Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Burton Sent: Thursday, February 22, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Microwave Cap Regulation I am curious to know how labs have responding to the CAP Question ANT.29430 which asks "Are microwave devices properly vented?". Have the labs continued to utilized their regular "kitchen model" microwaves and applied duct work to them to be vented out of the building? Please comment. Thank you, Stacey Burton, H.T. ASCP Precision Pathology Services San Antonio Texas ________________________________________________________________________ ____________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Don't get soaked. Take a quick peak at the forecast with the Yahoo! Search weather shortcut. http://tools.search.yahoo.com/shortcuts/#loc_weather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Feb 23 12:18:05 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Feb 23 12:18:16 2007 Subject: [Histonet] Extended time in paraffin In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C3F@LSRIEXCH1.lsmaster.lifespan.org> Personally I don't believe that a couple of days in formalin will "overfix" anything, and large fatty tissues like breast and colon specimens can only benefit from additional fixation time. That having been said, if you do want to limit the fixation time, why don't you program the processor to keep the tissue in the fixative in station 1 for the desired length of time, then go into an extended 70% ethanol in station 2, where the tissue can be held safely for as long as necessary before proceeding into 95% ethanol and the rest of the cycle on sunday night as usual. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Theresa Rohr > Sent: Friday, February 23, 2007 8:14 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Extended time in paraffin > > I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. > > The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! > > The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis > > 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? > 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? > 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? > > I have only found two clear commentaries. > Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. > > Can any of you offer me any further references and/or assistance or your own methods/experiences? > > Thank you so much for your time and assistance > Theresa Rohr > Nyack Hospital, NY > rohrt@nyackhospital.org > > Theresa Rohr, BA, HT(ASCP) > Section Head, Histology > Nyack Hospital > 160 North Midland Avenue > Nyack, New York 10960 > phone 845-348-2276 > fax 845-348-8430 > > Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From bhewlett <@t> cogeco.ca Fri Feb 23 12:29:11 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Feb 23 12:29:21 2007 Subject: [Histonet] Extended time in paraffin References: <4EBFF65383B74D49995298C4976D1D5E273C3F@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <008e01c75778$8478cdb0$6500a8c0@mainbox> Paul, Ahh! the voice of reason! No kidding, even 5-7 days or more won't 'overfix' because there is NO such thing as 'overfixation' in NBF. Bryan ----- Original Message ----- From: "Monfils, Paul" To: Sent: Friday, February 23, 2007 1:18 PM Subject: RE: [Histonet] Extended time in paraffin Personally I don't believe that a couple of days in formalin will "overfix" anything, and large fatty tissues like breast and colon specimens can only benefit from additional fixation time. That having been said, if you do want to limit the fixation time, why don't you program the processor to keep the tissue in the fixative in station 1 for the desired length of time, then go into an extended 70% ethanol in station 2, where the tissue can be held safely for as long as necessary before proceeding into 95% ethanol and the rest of the cycle on sunday night as usual. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Theresa Rohr > Sent: Friday, February 23, 2007 8:14 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Extended time in paraffin > > I have a problem with processing breast tissue over a weekend. Breast > tissue is run in a separate processor from our Sugical and endo specimens. > We do not have weekend staff. We usually run a two day delay with the > processing starting Sunday night and both processors ending early Monday > AM. > > The current guidelines suggest this delay of breast tissue sitting in > formalin could result in over fixed breast tissue. The pathologists want > to end the cycle on Saturday but we have no staff here to remove and/or > embed the tissue. The pathologists themselves would have to remove the > cassettes from the paraffin! > > The questions are what can they do with these cassettes? Again, the tissue > is breast tissue, mainly cores or target blocks or tumor that may need IHC > and/or FISH on diagnosis > > 1. Can they just leave the cassettes on the processor in warm paraffin > (60 degrees) from Saturday until Monday? > 2. Can they remove them from the paraffin and let them harden at room > temperature and then be heated, melted and properly embedded on Monday? > 3. Can they put the cassettes in a container of warm paraffin "to cover" > and then let the whole container solidify and on Monday Histo melts the > container, removes the cassettes and embeds? > > I have only found two clear commentaries. > Sheehan and Hrapchak who say extended time in paraffin will cause > shrinkage and hardening. There is also a reference in Carson, saying > tissue should remain in paraffin the shortest time necessary for good > infiltration as prolonged heat causes shrinkage and hardening. > > Can any of you offer me any further references and/or assistance or your > own methods/experiences? > > Thank you so much for your time and assistance > Theresa Rohr > Nyack Hospital, NY > rohrt@nyackhospital.org > > Theresa Rohr, BA, HT(ASCP) > Section Head, Histology > Nyack Hospital > 160 North Midland Avenue > Nyack, New York 10960 > phone 845-348-2276 > fax 845-348-8430 > > Nyack Hospital is required by Law to protect the privacy of health > information under HIPAA Rules and Regulations Part 11, Article 45 CFR, > Parts 160-164. This email, including attachments, is intended for the > exclusive use of the person or the entity to which it is addressed. It may > contain confidential or legally protected information. The authorized > recipient is prohibited from disclosing this information to any other > party and is required to destroy this information after its stated need > has been fulfilled. If the recipient or reader of this email is not the > intended recipient or her/his authorized agent, the reader is hereby > notified that any dissemination, distribution or copying of this email is > prohibited. If you believe that you have received this email in error, > please advise the sender immediately by reply email and then delete this > email immediately. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atebo <@t> aahs.org Fri Feb 23 12:52:40 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Fri Feb 23 12:52:54 2007 Subject: [Histonet] Her2 guidelines Message-ID: <00790F10D0600A41AEE8899901E533A61044B4E0@aamcexch.aamc.org> We hardly ever to Her2 on the large breast specimens. Only when we do not have the results from the core biopsy at our facility or the facility at which the core biopsy was done did not perform the testing. Most of our specimens for Her2 are the small core biopsies that usually go between that 6-48 hour time period anyway. Our Genzyme rep was actually here yesterday and she said that some of the labs are choosing to skip the IHC and go straight for the FISH. But from what I understand, the time limit also applies to Her2 by FISH as well. Is this correct? Andrea Tebo Supervisor Pathology Anne Arundel Medical Center 443-481-4240 ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From JWEEMS <@t> sjha.org Fri Feb 23 12:55:56 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 23 12:56:30 2007 Subject: [Histonet] Extended time in paraffin Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D350B@sjhaexc02.sjha.org> I believe this has to do with the new FDA ruling rather than "overfixing" as we think of it. It just has to do with standardization. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bryan Hewlett Sent: Friday, February 23, 2007 1:29 PM To: Monfils, Paul; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extended time in paraffin Paul, Ahh! the voice of reason! No kidding, even 5-7 days or more won't 'overfix' because there is NO such thing as 'overfixation' in NBF. Bryan ----- Original Message ----- From: "Monfils, Paul" To: Sent: Friday, February 23, 2007 1:18 PM Subject: RE: [Histonet] Extended time in paraffin Personally I don't believe that a couple of days in formalin will "overfix" anything, and large fatty tissues like breast and colon specimens can only benefit from additional fixation time. That having been said, if you do want to limit the fixation time, why don't you program the processor to keep the tissue in the fixative in station 1 for the desired length of time, then go into an extended 70% ethanol in station 2, where the tissue can be held safely for as long as necessary before proceeding into 95% ethanol and the rest of the cycle on sunday night as usual. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Theresa Rohr > Sent: Friday, February 23, 2007 8:14 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Extended time in paraffin > > I have a problem with processing breast tissue over a weekend. Breast > tissue is run in a separate processor from our Sugical and endo specimens. > We do not have weekend staff. We usually run a two day delay with the > processing starting Sunday night and both processors ending early Monday > AM. > > The current guidelines suggest this delay of breast tissue sitting in > formalin could result in over fixed breast tissue. The pathologists want > to end the cycle on Saturday but we have no staff here to remove and/or > embed the tissue. The pathologists themselves would have to remove the > cassettes from the paraffin! > > The questions are what can they do with these cassettes? Again, the tissue > is breast tissue, mainly cores or target blocks or tumor that may need IHC > and/or FISH on diagnosis > > 1. Can they just leave the cassettes on the processor in warm paraffin > (60 degrees) from Saturday until Monday? > 2. Can they remove them from the paraffin and let them harden at room > temperature and then be heated, melted and properly embedded on Monday? > 3. Can they put the cassettes in a container of warm paraffin "to cover" > and then let the whole container solidify and on Monday Histo melts the > container, removes the cassettes and embeds? > > I have only found two clear commentaries. > Sheehan and Hrapchak who say extended time in paraffin will cause > shrinkage and hardening. There is also a reference in Carson, saying > tissue should remain in paraffin the shortest time necessary for good > infiltration as prolonged heat causes shrinkage and hardening. > > Can any of you offer me any further references and/or assistance or your > own methods/experiences? > > Thank you so much for your time and assistance > Theresa Rohr > Nyack Hospital, NY > rohrt@nyackhospital.org > > Theresa Rohr, BA, HT(ASCP) > Section Head, Histology > Nyack Hospital > 160 North Midland Avenue > Nyack, New York 10960 > phone 845-348-2276 > fax 845-348-8430 > > Nyack Hospital is required by Law to protect the privacy of health > information under HIPAA Rules and Regulations Part 11, Article 45 CFR, > Parts 160-164. This email, including attachments, is intended for the > exclusive use of the person or the entity to which it is addressed. It may > contain confidential or legally protected information. The authorized > recipient is prohibited from disclosing this information to any other > party and is required to destroy this information after its stated need > has been fulfilled. If the recipient or reader of this email is not the > intended recipient or her/his authorized agent, the reader is hereby > notified that any dissemination, distribution or copying of this email is > prohibited. If you believe that you have received this email in error, > please advise the sender immediately by reply email and then delete this > email immediately. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pmcardle <@t> ebsciences.com Fri Feb 23 10:46:13 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Feb 23 13:05:57 2007 Subject: [Histonet] New Microwave Cap Regulation In-Reply-To: <878938.82563.qm@web30007.mail.mud.yahoo.com> References: <878938.82563.qm@web30007.mail.mud.yahoo.com> Message-ID: <45DF1A55.4070301@ebsciences.com> Here's a "vendor" reply: First of all, CAP's annual leakage measurement requirement was deleted 12.12.06. Second, should you still desire to check for leakage (always a good idea), a local appliance repair shop should be able to do it, but they may not wish to from a liability standpoint. There are also many sources for leakage measurement devices you can use yourself. The cheapest are prone to false positives; avoid "passive" type devices (don't require a battery) and "idiot-light" type. Expensive devices that "sum" and have built-in measurement spacers are available, but we feel are "overkill" for this application. Apologies for the following "shameless commerce," but EBS offers a "good enough" active (battery powered) handheld unit that provides a digital readout (model H2501) for $53, with instructions included that are tailored to a pathology lab context. Best regards, Phil McArdle -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway; please do the right thing and make this e-mail go away. Thank you. Stacey Burton wrote: > Thank you Hector. > This brings me to a new question - How do I test for leakage is less than 5mW/cm2 at a distance of 5 cm from the surface? Or what type of service to I call to help me with that? > -Stacey > > > ----- Original Message ---- > From: Hector Hernandez > To: Rene J Buesa ; "Luck, Greg D." ; Stacey Burton ; histonet@lists.utsouthwestern.edu > Sent: Thursday, February 22, 2007 4:19:54 PM > Subject: RE: [Histonet] New Microwave Cap Regulation > > > ANP.29430 Phase 1 > This question doesn't state laboratory or home grade microwaves. Microwave > should be placed in an appropriate ventilated hood to contain airborne > chemical contaminates and potential infectious agents. Microwaves used > outside a fume hood should have an integral fume extractor that is certified > by the manufacturer for use in a clinical laboratory. This doesn't say > microwave has to be laboratory grade, only the fume extractor. This > checklist question doesn't apply if only non-hazardous reagents are used in > the device (microwave) (e.g. water, certain biological stains) > > In my opinion you can use any microwave (laboratory or house microwave) as > long as you can document: > 1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface. > 2. Periodically monitor for temperature reproducibility. > 3. All containers used in the microwave are made from microwave-transparent > material/ microwave proof. > Don't make it any harder than it is. > > Hope this clears a few things, > > Hector > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, February 22, 2007 2:19 PM > To: Luck, Greg D.; Stacey Burton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New Microwave Cap Regulation > > Greg: > The new CAP regulation stems from the fact that many labs used the "house > microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, > manufacturers and a MW task force intervened with the valid warning that > fixing and processing, and even staining when the solutions are alcoholic, > are unsafe procedures to be done in a regular > HMWO,and that those procedures really require an instrument with a venting > capability that will take the noxious vapors out of the lab. > To just heat water or buffers for HIER I did that for many years and no > inspector ever said anything. Perhaps they have become more demanding > recently on this regulation, but for me there is nothing noxious to vent > when only water, PBS or buffers are heated. In your house you also heat > water and there is nothing noxious about it (and we are talking about the > "sacred" home environment). > Just my opinion though! > Ren? J. > > "Luck, Greg D." wrote: > Hello all, > Would venting or use of a "Laboratory Grade" microwave be required if > all that it is used for is to heat water or PBS or Tris buffers? > Thanks, Greg > Greg Luck, B.S., HT(ASCP) > Anatomic Pathology Supervisor > Deaconess Med Cntr > 800 W. 5th Ave > Spokane, WA 99204 > Offc 509.473.7077 > Fax 509.473.7133 > luckg@empirehealth.org > www.deaconessmc.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey > Burton > Sent: Thursday, February 22, 2007 8:22 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Microwave Cap Regulation > > I am curious to know how labs have responding to the CAP Question > ANT.29430 which asks "Are microwave devices properly vented?". > Have the labs continued to utilized their regular "kitchen model" > microwaves and applied duct work to them to be vented out of the > building? > Please comment. > > Thank you, > Stacey Burton, H.T. ASCP > Precision Pathology Services > San Antonio Texas > > > > ________________________________________________________________________ > ____________ > Food fight? Enjoy some healthy debate > in the Yahoo! Answers Food & Drink Q&A. > http://answers.yahoo.com/dir/?link=list&sid=396545367 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Now that's room service! Choose from over 150,000 hotels > in 45,000 destinations on Yahoo! Travel to find your fit. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ____________________________________________________________________________________ > Don't get soaked. Take a quick peak at the forecast > with the Yahoo! Search weather shortcut. > http://tools.search.yahoo.com/shortcuts/#loc_weather > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kjohnson <@t> covx.com Fri Feb 23 13:17:26 2007 From: kjohnson <@t> covx.com (Kimberly Johnson) Date: Fri Feb 23 13:17:34 2007 Subject: [Histonet] random PFA filtering question In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E127CE@VHAV20MSGA3.v20.med.va.gov> Message-ID: Hi, When I make my 4% PFA I usually filter with the little coffee filter looking things. Today I decided to use the vacuum filter that I use for my cell culture media for sterile filtering so it would go faster. I noticed that it doesn't have that really strong PFA smell that I am used to. Did I ruin my PFA, or is it ok? Thanks so much! Kim Kimberly Johnson Research Associate II CovX Ph. (858)964-2050 Fax (858)964-2090 The information contained in this e-mail message may be privileged and confidential information and is intended only for the use of the individual and/or entity identified in the alias address of this message. If the reader of this message is not the intended recipient, or an employee or agent responsible to deliver it to the intended recipient, you are hereby requested not to distribute or copy this communication. If you have received this communication in error, please notify us immediately by telephone or return e-mail and delete the original message from your system. From LINDA.MARGRAF <@t> childrens.com Fri Feb 23 13:18:47 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri Feb 23 13:19:07 2007 Subject: [Histonet] lab manager position Message-ID: <45DEE9B7020000DA00006373@CNET3.CHILDRENS.COM> Dear Histonetters; Here is a job opportunity I was asked to put on the list. Please contact Myrle Cummings (info below) and not me if you are interested. Thanks. Linda M Histonet administrator Histology Laboratory Manager Washington Animal Disease Diagnostic Laboratory College of Veterinary Medicine, Pullman, WA The histology laboratory in the Washington Disease Diagnostic Laboratory (WADDL) is currently seeking applicants for the full-time position of histology laboratory manager. This position directs the histology laboratory operations and involves staff supervision and oversight of histology procedures, budget planning, and laboratory policies. The WADDL is associated with the WSU College of Veterinary Medicine and multiple university research programs both internal and external to Washington State University. The manager is expected to perform routine and special histological procedures as well as be on the forefront of new procedures, tests, and services needed for WADDL clients. MINIMUM QUALIFICATIONS Position requires a Bachelor?s degree in a relevant field and four (4) years of progressively responsible experience as a working histotechnician or histotechnologist, which has included at least one (1) year of supervisory experience. Any combination of relevant education and experience may be substituted for educational requirement on a year-for-year basis. Experience working as a histotechnologist in high volume setting. Supervisory experience in a multiple-employee laboratory setting. PREFERRED QUALIFICATIONS ASCP Certification as a Histotechnician or Histotechnologist. (While ASCP certification is not required to qualify for this position, applicant is expected to have the qualifications and obtain ASCP certification within 1 year of hire.) Experience in standard operating procedure (SOP) development and implementation for laboratory quality assurance and quality control practices. Familiarity with accredited histology laboratory procedures, including working in Biosafety Level 2 facilities. Experience with the coordination and implementation of laboratory health and environmental safety programs SALARY: Salary is commensurate with candidate?s training, professional qualifications, and experience. APPLICATION: Screening of application materials will begin April 1, 2007. Please send a letter of application addressing all minimum and preferred qualifications for this position, a r?sum? and contact information for three professional references (include name, addresses, telephone numbers and email addresses) to: Ms. Myrle Cummings Washington Animal Disease Diagnostic Laboratory Washington State University PO Box 647034 Pullman, WA 99164-7034 Phone: 509-335-3374, Fax: 335-7424 or mlc@vetmed.wsu.edu A complete position description may be requested via mail, fax or email. WASHINGTON STATE UNIVERSITY IS AN EQUAL OPPORTUNITY/AFFIRMATIVE ACTION EDUCATOR AND EMPLOYER. Members of ethnic minorities, women, Vietnam-era disabled veterans, persons of disability, and/or persons age 40 and over are encouraged to apply. WSU employs only U.S. citizens and lawfully authorized non-U.S. citizens. All new employees must show employment eligibility verification as required by the U.S. Citizenship and Immigration Services. From bhewlett <@t> cogeco.ca Fri Feb 23 13:19:43 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Feb 23 13:19:51 2007 Subject: [Histonet] Extended time in paraffin References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D350B@sjhaexc02.sjha.org> Message-ID: <00a201c7577f$928855e0$6500a8c0@mainbox> Joyce, The original questioner mentioned so called 'overfixation' and asked how to prevent the possibility. If you, or anyone else, think that a fixation time just anywhere in the wide range of 8-48 hours constitutes standardization, the word needs to be totally redefined!!! Bryan ----- Original Message ----- From: "Weems, Joyce" To: "Bryan Hewlett" ; "Monfils, Paul" ; Sent: Friday, February 23, 2007 1:55 PM Subject: RE: [Histonet] Extended time in paraffin >I believe this has to do with the new FDA ruling rather than "overfixing" >as we think of it. It just has to do with standardization. j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bryan > Hewlett > Sent: Friday, February 23, 2007 1:29 PM > To: Monfils, Paul; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extended time in paraffin > > > Paul, > > Ahh! the voice of reason! > No kidding, even 5-7 days or more won't 'overfix' because there is NO such > thing as 'overfixation' in NBF. > > > Bryan > > > ----- Original Message ----- > From: "Monfils, Paul" > To: > Sent: Friday, February 23, 2007 1:18 PM > Subject: RE: [Histonet] Extended time in paraffin > > > Personally I don't believe that a couple of days in formalin will > "overfix" > anything, and large fatty tissues like breast and colon specimens can only > benefit from additional fixation time. That having been said, if you do > want > to limit the fixation time, why don't you program the processor to keep > the > tissue in the fixative in station 1 for the desired length of time, then > go > into an extended 70% ethanol in station 2, where the tissue can be held > safely for as long as necessary before proceeding into 95% ethanol and the > rest of the cycle on sunday night as usual. > >> ---------- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Theresa Rohr >> Sent: Friday, February 23, 2007 8:14 AM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Extended time in paraffin >> >> I have a problem with processing breast tissue over a weekend. Breast >> tissue is run in a separate processor from our Sugical and endo >> specimens. >> We do not have weekend staff. We usually run a two day delay with the >> processing starting Sunday night and both processors ending early Monday >> AM. >> >> The current guidelines suggest this delay of breast tissue sitting in >> formalin could result in over fixed breast tissue. The pathologists want >> to end the cycle on Saturday but we have no staff here to remove and/or >> embed the tissue. The pathologists themselves would have to remove the >> cassettes from the paraffin! >> >> The questions are what can they do with these cassettes? Again, the >> tissue >> is breast tissue, mainly cores or target blocks or tumor that may need >> IHC >> and/or FISH on diagnosis >> >> 1. Can they just leave the cassettes on the processor in warm paraffin >> (60 degrees) from Saturday until Monday? >> 2. Can they remove them from the paraffin and let them harden at room >> temperature and then be heated, melted and properly embedded on Monday? >> 3. Can they put the cassettes in a container of warm paraffin "to cover" >> and then let the whole container solidify and on Monday Histo melts the >> container, removes the cassettes and embeds? >> >> I have only found two clear commentaries. >> Sheehan and Hrapchak who say extended time in paraffin will cause >> shrinkage and hardening. There is also a reference in Carson, saying >> tissue should remain in paraffin the shortest time necessary for good >> infiltration as prolonged heat causes shrinkage and hardening. >> >> Can any of you offer me any further references and/or assistance or your >> own methods/experiences? >> >> Thank you so much for your time and assistance >> Theresa Rohr >> Nyack Hospital, NY >> rohrt@nyackhospital.org >> >> Theresa Rohr, BA, HT(ASCP) >> Section Head, Histology >> Nyack Hospital >> 160 North Midland Avenue >> Nyack, New York 10960 >> phone 845-348-2276 >> fax 845-348-8430 >> >> Nyack Hospital is required by Law to protect the privacy of health >> information under HIPAA Rules and Regulations Part 11, Article 45 CFR, >> Parts 160-164. This email, including attachments, is intended for the >> exclusive use of the person or the entity to which it is addressed. It >> may >> contain confidential or legally protected information. The authorized >> recipient is prohibited from disclosing this information to any other >> party and is required to destroy this information after its stated need >> has been fulfilled. If the recipient or reader of this email is not the >> intended recipient or her/his authorized agent, the reader is hereby >> notified that any dissemination, distribution or copying of this email is >> prohibited. If you believe that you have received this email in error, >> please advise the sender immediately by reply email and then delete this >> email immediately. >> >> > >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > From tkngflght <@t> yahoo.com Fri Feb 23 12:25:31 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri Feb 23 13:25:43 2007 Subject: [Histonet] KOH in processing for Nail fungus In-Reply-To: <9E6D52F532809247BDA1783680E92C560B70279E@CHWEXC.chwi.chswi.org> References: <9E6D52F532809247BDA1783680E92C560B70279E@CHWEXC.chwi.chswi.org> Message-ID: <009301c75778$0136a940$6401a8c0@FSDESKTOP> Nail biters anonymous-- I haven't been following this conversation so if this solution has already been posted, I apologize for the repeat. The best nail processing I've ever experienced first hand was in a Texas lab last year and again in another lab in the Northeast--so it is very reproducible. They grossed the nail, soaked the cassette with the representative sections in a low percentage (I think it was 10%) of Potassium Hydroxide for one hour (no more than 90 minutes), rinsed and added to the processors with all the rest. They cut like BUTTER to where I'd VOLUNTEER to cut nails. They stayed on the charged slides even during the PAS water rinses...amazing. It did not interfere with PAS staining but I can't speak to any IHC testing. The only negative I saw was if you forgot to pull them they'd miss the run and maybe get squishy. I'll try to find the actual percentage for you--but 10% is what sticks in my head--must be all the cobwebs? Happy Friday! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From KMENDELL <@t> nhs-healthlink.org Fri Feb 23 14:06:36 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Fri Feb 23 14:06:56 2007 Subject: [Histonet] Does BerEP4 go by any other name and who makes it? Message-ID: <45DF02FC020000A8000117F6@mail.NHS-HEALTHLINK.ORG> Does BerEP4 go by any other name and who makes it? ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From jnocito <@t> satx.rr.com Fri Feb 23 14:22:29 2007 From: jnocito <@t> satx.rr.com (jnocito@satx.rr.com) Date: Fri Feb 23 14:22:38 2007 Subject: [Histonet] "Joe The Toe" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB492@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB492@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: no, no, no. I was a running back when I played high school football (American not soccor). I lasted one year because I was a small guy and the linebackers were eating me for a snack. I decided that I had to do something else because I was going to get killed (actually, my knee got messed up a couple of times). After quitting for health reasons, I realized that I could've become a place kicker. You know kick extra points and fieldgoals. I could have become "Joe The Toe". Now, I'm JTT for a different reason, a reason that would never cross my mind in a million years. Boy I need a Scotch. JTT ----- Original Message ----- From: "Breeden, Sara" Date: Friday, February 23, 2007 11:18 am Subject: [Histonet] "Joe The Toe" To: histonet@lists.utsouthwestern.edu > Well, that clarifies THAT! And what a relief, as I had other reasons > for that nickname in MY mind! > > > > TGIF and then some! Anybody need to Fume? > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From awatanabe <@t> tgen.org Fri Feb 23 14:22:37 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Fri Feb 23 14:22:44 2007 Subject: [Histonet] Re: Vision Biosystems Bondmax for immunostaining In-Reply-To: <20070223175014.C6A8A202464E@mr1.tgen.org> Message-ID: I use the BondMax from Vision Biosystems. I really like the machine, it's very reproducible and great for a clinical/hospital lab. The Refine detection kit is very good. You can use less reagents than some of the other systems out there. I evaluated the BondMax against the Nemesis from Biocare (similar to DAKO platform) and found that we could get more specific staining with the BondMax and push our titers out further. I would recommend the BondMax. I hope this helps some. > > Message: 19 > Date: Fri, 23 Feb 2007 11:46:42 -0600 > From: "Foshey, Annette" > Subject: [Histonet] Lab Vision Bond system for Immunostaining > To: > Message-ID: > <9E6D52F532809247BDA1783680E92C560B70279E@CHWEXC.chwi.chswi.org> > Content-Type: text/plain; charset="us-ascii" > > I am in the process of evaluating immuno stainers and would like input > from users of the Bond system as well as other stainers. > > Thank you, > Annette Foshey, HT > Histology Lab > Children's Hospital of Wisconsin > > ************************** > > Children's Hospital and Health System recognizes that unencrypted e-mail is > insecure and does not guarantee confidentiality. The confidentiality of > replies to this message cannot be guaranteed unless the replies are encrypted. > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 39, Issue 37 > **************************************** Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From rjbuesa <@t> yahoo.com Fri Feb 23 14:25:29 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 23 14:25:36 2007 Subject: [Histonet] Extended time in paraffin In-Reply-To: Message-ID: <229452.25494.qm@web61222.mail.yahoo.com> I have never liked a cassette with the tissue to harden by itself. I would recommend your option 3 The reference is my personal experience. Ren? J. Theresa Rohr wrote: I have a problem with processing breast tissue over a weekend. Breast tissue is run in a separate processor from our Sugical and endo specimens. We do not have weekend staff. We usually run a two day delay with the processing starting Sunday night and both processors ending early Monday AM. The current guidelines suggest this delay of breast tissue sitting in formalin could result in over fixed breast tissue. The pathologists want to end the cycle on Saturday but we have no staff here to remove and/or embed the tissue. The pathologists themselves would have to remove the cassettes from the paraffin! The questions are what can they do with these cassettes? Again, the tissue is breast tissue, mainly cores or target blocks or tumor that may need IHC and/or FISH on diagnosis 1. Can they just leave the cassettes on the processor in warm paraffin (60 degrees) from Saturday until Monday? 2. Can they remove them from the paraffin and let them harden at room temperature and then be heated, melted and properly embedded on Monday? 3. Can they put the cassettes in a container of warm paraffin "to cover" and then let the whole container solidify and on Monday Histo melts the container, removes the cassettes and embeds? I have only found two clear commentaries. Sheehan and Hrapchak who say extended time in paraffin will cause shrinkage and hardening. There is also a reference in Carson, saying tissue should remain in paraffin the shortest time necessary for good infiltration as prolonged heat causes shrinkage and hardening. Can any of you offer me any further references and/or assistance or your own methods/experiences? Thank you so much for your time and assistance Theresa Rohr Nyack Hospital, NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From JPCOLEMA <@t> sentara.com Fri Feb 23 14:35:08 2007 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Fri Feb 23 14:35:39 2007 Subject: [Histonet] Re: Histonet Digest, Vol 39, Issue 38 In-Reply-To: <200702231826.l1NIQAf23019@sdclin01.sdc.sentara.com> References: <200702231826.l1NIQAf23019@sdclin01.sdc.sentara.com> Message-ID: 1) Breast processing- pulling them out of paraffin and leaving them to harden in cassettes is what I have done with many tissues with no ill effect. Alternatively, you can fix in formalin for the minimum 6 hours in a tray in the grossing room, and since you have a dedicated processor, you can take the formalin out of the first processing station and replace it with a 70% alcohol holding solution, set your second station (formalin) for 12 hours, and have a fixation time of about 18 hours. The first 6 hours of formalin fixation should guard against any coagulative fixative effect of the 70% step, and 70% alc is the recommended post fix holding solution to prevent over fixation issues. Set the endtime for when you need em out and you should be good to go. 2) Microwave testing- I bought 4 testers for 64 bucks each from Lab Safety Supply part number 27879, phone #1800-240-6373. We test monthly. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)287-6657 pager: (757)456-6695 office:-(757)388-3295 From tmhallada <@t> gratiothealth.com Fri Feb 23 14:53:49 2007 From: tmhallada <@t> gratiothealth.com (Teresa M Hallada) Date: Fri Feb 23 14:54:45 2007 Subject: [Histonet] Grossing high complexity testing Message-ID: Does anyone know if "Grossing" is considered high complexity testing only by CAP and not by CLIAA or JACHO? Teri Hallada MTCT (ASCP) From ElizabethWyand <@t> texashealth.org Fri Feb 23 15:26:18 2007 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Fri Feb 23 15:26:29 2007 Subject: [Histonet] PA weekend coverage Message-ID: <26BE9ACC202D29479B0A144066A733E50223FE2F@phdex01.txhealth.org> Looking for some extra spending money? We are looking for additional Sunday PA gross coverage in our Plano, TX lab. Very competitive pay. Interested parties - please contact Kip Asbury or Alison Harden at 972.981.8065 or kipasbury@texashealth.org alisonharden@texashealth.org Thanks and Happy Friday! The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From ElizabethWyand <@t> texashealth.org Fri Feb 23 15:52:58 2007 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Fri Feb 23 15:53:06 2007 Subject: [Histonet] Histotech needed Message-ID: <26BE9ACC202D29479B0A144066A733E50223FE32@phdex01.txhealth.org> PRN histotech needed for our Plano, TX lab due to retiring tech. Interested parties please contact Genie at 972.981.3108 or geniejacobs@texashealth.org The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Charles.Embrey <@t> carle.com Fri Feb 23 16:05:26 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Feb 23 16:05:39 2007 Subject: [Histonet] Grossing high complexity testing In-Reply-To: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4B7@EXCHANGEBE1.carle.com> CLIA '88 considers all grossing to be "high complexity testing". CAP, just last year, has made a statement that they consider small specimens to be considered less than grossing and therefore not high complexity testing. CAP still considers larger specimens as grossing. The problem you run into is that if you are required to have CLIA certification as well as CAP you must still meet the requirements for CLIA. As far as I know JACHO is staying out of the conflict. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa M Hallada Sent: Friday, February 23, 2007 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing high complexity testing Does anyone know if "Grossing" is considered high complexity testing only by CAP and not by CLIAA or JACHO? Teri Hallada MTCT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Fri Feb 23 16:52:56 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Feb 23 16:53:07 2007 Subject: [Histonet] Does BerEP4 go by any other name and who makes it? Message-ID: <022320072252.22858.45DF70480005224F0000594A22007636929D09020704040A0105@comcast.net> Kate, Its tricky for a specific synonym but I believe BerEP4 (specificity uncharacterized) will turn out to be a clone that describes an epitope of what some call Ep-CAM (epithelial cellular adhesion molecule). Look for Ep-CAM in Lab Vision or Abcam sites and they describe the 25+ and growing synonyms to Ep-CAM, BerEP4 being one of them. This is a glycoprotein in epithelia that was discovered in the 70's and with each new "discovery" gets another name. Sort of like when you were reading journal articles in the 80's, all of which seemed curiously to be talking about the same cell or cellular phenotype, but each researcher having their own designation and it was utterly confusing. Until they got the CD workshops going in the 80's and figured out that hundreds of labs, all calling their antibody clone or target by their own name, were actually describing the very same target (CD4, or CD5 or CD8, etc). No one has taken the time to do such efficient catagorization with the molecules you a re asking about but they are increasingly interesting to cancer biologists. So I think this nomenclature can of worms is soon to be worked out. CD326 (EpCAM) is now a current nomenclature I believe. It turns out that a clone that we developed and I worked on in grad school, that stained the surface of cells of high endothelial venules in lymph nodes, probably was just our name for this very target and we didn't realize it. Ray no affiliation or employer yet (but almost do now so my golf playing free time is about over) Seattle, WA -------------- Original message -------------- From: "Kate Mendell" > Does BerEP4 go by any other name and who makes it? > > Øòis message and its contents are confidential and are intended for the > use of the addressee only, and may contain information that is > privileged, confidential and exempt from disclosure under applicable > law. If you are not the intended recipient, this serves as notice that > any unauthorized distribution, duplication, printing, or any other use > is strictly prohibited. If you feel you have received this email in > error, please delete the message and notify the sender so that we may > prevent future occurrences. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Fri Feb 23 17:10:08 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Feb 23 17:10:17 2007 Subject: [Histonet] lab manager position In-Reply-To: <45DEE9B7020000DA00006373@CNET3.CHILDRENS.COM> References: <45DEE9B7020000DA00006373@CNET3.CHILDRENS.COM> Message-ID: <45DF7450.5040408@pathology.washington.edu> I thought I'd throw in my .02. I was the manager there from 1990-99. WADDL is a great organization, staffed with exceptional people. The workload was very diverse among species and there was a broad range of research projects. This is an academic facility with residents in training. The only reason I left was to relocate near the grandkids. This is small college town living, population approx. 30,000. The summers are very quiet with the students gone. For you sports fans, Cougar basketball is currently ranked #10 and the football team holds its' own in the PAC-10. The closest big town is Spokane to the north. Definite 4 season weather. Feel free to contact me if you have any questions. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. LINDA MARGRAF wrote: > Dear Histonetters; > Here is a job opportunity I was asked to put on the list. Please contact Myrle Cummings (info below) and not me if you are interested. Thanks. > Linda M > Histonet administrator > > > Histology Laboratory Manager > Washington Animal Disease Diagnostic Laboratory > College of Veterinary Medicine, Pullman, WA > > The histology laboratory in the Washington Disease Diagnostic Laboratory (WADDL) is currently seeking applicants for the full-time position of histology laboratory manager. This position directs the histology laboratory operations and involves staff supervision and oversight of histology procedures, budget planning, and laboratory policies. The WADDL is associated with the WSU College of Veterinary Medicine and multiple university research programs both internal and external to Washington State University. The manager is expected to perform routine and special histological procedures as well as be on the forefront of new procedures, tests, and services needed for WADDL clients. > > MINIMUM QUALIFICATIONS > Position requires a Bachelor?s degree in a relevant field and four (4) years of progressively responsible experience as a working histotechnician or histotechnologist, which has included at least one (1) year of supervisory experience. Any combination of relevant education and experience may be substituted for educational requirement on a year-for-year basis. Experience working as a histotechnologist in high volume setting. Supervisory experience in a multiple-employee laboratory setting. > > PREFERRED QUALIFICATIONS > ASCP Certification as a Histotechnician or Histotechnologist. (While ASCP certification is not required to qualify for this position, applicant is expected to have the qualifications and obtain ASCP certification within 1 year of hire.) Experience in standard operating procedure (SOP) development and implementation for laboratory quality assurance and quality control practices. Familiarity with accredited histology laboratory procedures, including working in Biosafety Level 2 facilities. Experience with the coordination and implementation of laboratory health and environmental safety programs > > SALARY: Salary is commensurate with candidate?s training, professional qualifications, and experience. > > APPLICATION: Screening of application materials will begin April 1, 2007. > > Please send a letter of application addressing all minimum and preferred qualifications for this position, a r?sum? and contact information for three professional references (include name, addresses, telephone numbers and email addresses) to: > > Ms. Myrle Cummings > Washington Animal Disease Diagnostic Laboratory > Washington State University > PO Box 647034 > Pullman, WA 99164-7034 > Phone: 509-335-3374, Fax: 335-7424 or mlc@vetmed.wsu.edu > > A complete position description may be requested via mail, fax or email. > > > WASHINGTON STATE UNIVERSITY IS AN EQUAL > OPPORTUNITY/AFFIRMATIVE ACTION EDUCATOR AND EMPLOYER. > Members of ethnic minorities, women, Vietnam-era disabled veterans, persons > of disability, and/or persons age 40 and over are encouraged to apply. WSU employs only U.S. citizens and lawfully authorized non-U.S. citizens. All new employees must show employment eligibility verification as required by the U.S. Citizenship and Immigration Services. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From es144131 <@t> bcm.tmc.edu Fri Feb 23 18:21:44 2007 From: es144131 <@t> bcm.tmc.edu (Stephens, Elizabeth Humes) Date: Fri Feb 23 18:21:58 2007 Subject: [Histonet] varying time in formalin Message-ID: <10C24F7C4D05EB45B5F0E1B3978978490131F0C3@BCMEVS7.ad.bcm.edu> Hi everyone! I am wondering whether anyone has looked at how much length of time in formalin affects the ability to do immunohistochemistry? For instance, I want to do a pathology study on heart valves but have hearts that have been in formalin 1 year, 2 years, 3 years and am afraid this will affect my results. I would be interested in IHC staining of matrix markers including various collagen types, elastin, fibrillin, proteoglycans, glycoaminoglycans, smooth muscle alpha actin, lysyl oxidase, prolyl 4-hydroxylase. Thank you!! From jcolclefa <@t> aol.com Fri Feb 23 18:45:27 2007 From: jcolclefa <@t> aol.com (John PJ Coleman) Date: Fri Feb 23 18:46:18 2007 Subject: [Histonet] Re: Histonet Digest, Vol 39, Issue 35 In-Reply-To: <200702230625.1b045decf391af@rly-yc01.mail.aol.com> References: <200702230625.1b045decf391af@rly-yc01.mail.aol.com> Message-ID: <8D1DF9FF-A135-4C3D-B5C8-DEEFDDC2C360@aol.com> 1) Nail Fungus: In our lab we use PAS with a light green counterstain. We soften them in "Nair" first. 10 min 1% periodic acid, rinse DIH2O, 20 min schiff reagent, Develop in warm standing tap water, 2% Alc Lt green solution 2-3 min, run down , cover. Charged slides so they don't wash off. 2) ER/PR- our docs get nervous if the control isn't at least 50% strongly pos. The best option is a mixed block with multiple cases of varying expression. Embed 6 slivers from 6 separate cases, document your "reactivity map" include liver or tonsil which should always be negative, and voila! multiple ranges and neg tissue control on one slide. 3) Microwave venting: Our microwave processors are vented to outside. If the stains you use in the microwave include AFB (Carbol fuchsin=phenolic) PAS (schiff= sulfite) or GMS (methenamine) then you may have to at least use them under a hood. 4) IHC/DAB- I've coverslipped DAB IHC's after drying well with no ill effect, no alcohol or xylene. 5) Cheese brains- If your primary freeze produces no artefact, (cheese holes) then holding at a colder temp after adding OCT will not create them. Your sucrose treatment already prevents big ice crystals from forming in the brain/producing cheese holes when you freeze in the cryostat. (Wood frogs, Rana sylvatica, hibernate completely frozen using this method, defrost in the spring and walk away with no cheesy brain holes) The faster the freeze, the smaller the crystal. We freeze in isopentane cooled with LN2, then add OCT, then store @ -70 and get no freeze cheese. 6) Recycler- Anatech sells buffer packs for recycled formalin, and FDA doesn't certify alcohol, xylene or formalin, but may restrict the use of such in FDA approved procedures, such as Herceptest or PathVysion, etc. Check the package inserts for your FDA approved tests to see if recycled stuff is allowed or not. PS- recycling formalin= bad idea. The theory doesn't make sense to me. It isn't a solvent, formaldeyhde molecules are lost in fixation to the tissue, is't more pain than it's worth. I liken it to distilling urine to save on distilled water costs. I'd much rather spend that time on making my hematoxylin. Now THAT's fun! From bhewlett <@t> cogeco.ca Fri Feb 23 19:08:51 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Feb 23 19:09:02 2007 Subject: [Histonet] Does BerEP4 go by any other name and who makes it? References: <45DF02FC020000A8000117F6@mail.NHS-HEALTHLINK.ORG> Message-ID: <002001c757b0$5954f8b0$6500a8c0@mainbox> Kate, Dako lists this antibody as Epithelial Antigen clone Ber-EP4. I have used this product for a number of years, it works well. Bryan ----- Original Message ----- From: "Kate Mendell" To: Sent: Friday, February 23, 2007 3:06 PM Subject: [Histonet] Does BerEP4 go by any other name and who makes it? Does BerEP4 go by any other name and who makes it? ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ancillarypath <@t> mac.com Fri Feb 23 19:13:50 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Fri Feb 23 19:14:02 2007 Subject: [Histonet] Re: Her2 guidelines In-Reply-To: <200702232317.l1NNHSbF023373@mac.com> References: <200702232317.l1NNHSbF023373@mac.com> Message-ID: Dear Andrea, The fixation guidelines are applicable to specimen processing and fixation, regardless of the test. If your lab opts to do FISH as a first-line test, you will still be require to adhere by the guidelines by the end of 2007. What was stated by your rep is a misconception. If labs decide to go straight to FISH, they will still have to abide by all the requirements set forth by the CAP/ASCO task force. Best regards, ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com > Message: 3 > Date: Fri, 23 Feb 2007 13:52:40 -0500 > From: "Tebo, Andrea" > Subject: [Histonet] Her2 guidelines > To: > Message-ID: > <00790F10D0600A41AEE8899901E533A61044B4E0@aamcexch.aamc.org> > Content-Type: text/plain; charset="us-ascii" > > > We hardly ever to Her2 on the large breast specimens. Only when we do > not have the results from the core biopsy at our facility or the > facility at which the core biopsy was done did not perform the > testing. > Most of our specimens for Her2 are the small core biopsies that > usually > go between that 6-48 hour time period anyway. Our Genzyme rep was > actually here yesterday and she said that some of the labs are > choosing > to skip the IHC and go straight for the FISH. But from what I > understand, the time limit also applies to Her2 by FISH as well. Is > this correct? > > Andrea Tebo > Supervisor Pathology > Anne Arundel Medical Center > 443-481-4240 From pathrm35 <@t> charter.net Fri Feb 23 23:56:53 2007 From: pathrm35 <@t> charter.net (pathrm35@charter.net) Date: Fri Feb 23 23:57:02 2007 Subject: [Histonet] Re: Vision Biosystems Bondmax for immunostaining Message-ID: <1132468236.1172296613600.JavaMail.root@fepweb12> I had an opportunity to set up a lab in Florida in which we had the Bond IHC stainer. The Florida field service specialist, Steve Westra, is excellent. He was there for about two weeks straight setting everything up. He actually showed up at 10 PM one night to fix a problem!!! The equipment is very good with good staining results and easy to use. I also went to their headquarters in Mass. for a week of training.They were all very professional and very focused on their training. I would recommend this product and company including their other products such as the Peloris tissue processor. Ron Martin --- Aprill Watanabe wrote: > I use the BondMax from Vision Biosystems. I really like the machine, it's > very reproducible and great for a clinical/hospital lab. The Refine > detection kit is very good. You can use less reagents than some of the > other systems out there. I evaluated the BondMax against the Nemesis from > Biocare (similar to DAKO platform) and found that we could get more specific > staining with the BondMax and push our titers out further. I would > recommend the BondMax. I hope this helps some. > > > > > > > Message: 19 > > Date: Fri, 23 Feb 2007 11:46:42 -0600 > > From: "Foshey, Annette" > > Subject: [Histonet] Lab Vision Bond system for Immunostaining > > To: > > Message-ID: > > <9E6D52F532809247BDA1783680E92C560B70279E@CHWEXC.chwi.chswi.org> > > Content-Type: text/plain; charset="us-ascii" > > > > I am in the process of evaluating immuno stainers and would like input > > from users of the Bond system as well as other stainers. > > > > Thank you, > > Annette Foshey, HT > > Histology Lab > > Children's Hospital of Wisconsin > > > > ************************** > > > > Children's Hospital and Health System recognizes that unencrypted e-mail is > > insecure and does not guarantee confidentiality. The confidentiality of > > replies to this message cannot be guaranteed unless the replies are encrypted. > > > > > > ------------------------------ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > End of Histonet Digest, Vol 39, Issue 37 > > **************************************** > > Aprill Watanabe, B.S. > Research Associate > Tissue Microarray Center (TMA) > Translational Genomics Research Institute (TGen) > 602-343-8822 > awatanabe@tgen.org > www.tgen.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Feb 24 02:49:25 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 24 02:49:28 2007 Subject: AW: [Histonet] varying time in formalin In-Reply-To: <10C24F7C4D05EB45B5F0E1B3978978490131F0C3@BCMEVS7.ad.bcm.edu> Message-ID: <000101c757f0$b092d990$6412a8c0@dielangs.at> Yes, it makes a difference how long the tissue was in formalin, especially as you say for years. I would recommand to stain the tissue first with an antibody, that has to have a positiv result and look how the tissue reacts. In a workshop the trainer showed us biopsies for a study, that were in formalin for years. She had to do a very crued antigenretrieval to get the ihc working (I'm sorry I don't remember the detailled protocol). Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stephens, Elizabeth Humes Gesendet: Samstag, 24. Februar 2007 01:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] varying time in formalin Hi everyone! I am wondering whether anyone has looked at how much length of time in formalin affects the ability to do immunohistochemistry? For instance, I want to do a pathology study on heart valves but have hearts that have been in formalin 1 year, 2 years, 3 years and am afraid this will affect my results. I would be interested in IHC staining of matrix markers including various collagen types, elastin, fibrillin, proteoglycans, glycoaminoglycans, smooth muscle alpha actin, lysyl oxidase, prolyl 4-hydroxylase. Thank you!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Feb 24 02:58:21 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 24 02:58:19 2007 Subject: AW: [Histonet] Extended time in paraffin In-Reply-To: <00a201c7577f$928855e0$6500a8c0@mainbox> Message-ID: <000201c757f1$f0235250$6412a8c0@dielangs.at> Isn't it a little bit contraproductive to invite a bigger number of varying protocols to meet the required fixation time? I always thought standardization is the point that matters. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 From vanann702 <@t> skmc.gov.ae Sat Feb 24 05:34:17 2007 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Sat Feb 24 05:34:10 2007 Subject: [Histonet] extended time in paraffin Message-ID: For anything which is longer than the normal overnight processing, I have programmed our VIP5 for the tissue to spend 12 hours in formalin, then they stay in 70% alcohol for an extended period of time before the rest of the normal processing programme kicks in I prefer not to leave tissue in paraffin wax for too long - so the extra time is allocated to the 70% have had no problems at all with breast bx's or subsequent IHC Annie From rjbuesa <@t> yahoo.com Sat Feb 24 08:10:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 24 08:10:16 2007 Subject: [Histonet] varying time in formalin In-Reply-To: <10C24F7C4D05EB45B5F0E1B3978978490131F0C3@BCMEVS7.ad.bcm.edu> Message-ID: <647525.74774.qm@web61218.mail.yahoo.com> Elizabeth: As you well know, croslinkage by formalin during fixation (as well as xylene as an antemedium) has cause the development of the Heat Induced Epitope Retrieval (HIER) and, although there is not such a thing as "overfixation", many consider that the longer a tissue is in formalin, the more IHC is "affected". What I would suggest you to do is to get a fresh specimen, fix it for just 6 hours, process it and use it as a positive control against the specimen kept the longest in formalin. Then you will be able to see the difference, if any at all! Just a thoght! Ren? J. "Stephens, Elizabeth Humes" wrote: Hi everyone! I am wondering whether anyone has looked at how much length of time in formalin affects the ability to do immunohistochemistry? For instance, I want to do a pathology study on heart valves but have hearts that have been in formalin 1 year, 2 years, 3 years and am afraid this will affect my results. I would be interested in IHC staining of matrix markers including various collagen types, elastin, fibrillin, proteoglycans, glycoaminoglycans, smooth muscle alpha actin, lysyl oxidase, prolyl 4-hydroxylase. Thank you!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. From Laurie <@t> conxis.com Sat Feb 24 08:20:35 2007 From: Laurie <@t> conxis.com (Laurie Popp) Date: Sat Feb 24 08:20:44 2007 Subject: [Histonet] PAS stain for nail fungus Message-ID: <003e01c7581e$f3f150d0$9700a8c0@laurie> We also frequently cut toenails for PAS Staining among others.... we use the liquid dial handsoap from our handwashing area mixed with very little water to make a paste consistency and soak the block in that for about 20 minutes and then cut at our workstations... works like a charm and doesn't affect staining. Laurie Popp HT Candidate, Mayo Clinic From Laurie <@t> conxis.com Sat Feb 24 08:24:01 2007 From: Laurie <@t> conxis.com (Laurie Popp) Date: Sat Feb 24 08:24:09 2007 Subject: [Histonet] PAS stain for nail fungus Message-ID: <004301c7581f$6ef15a50$9700a8c0@laurie> Woops it's early I forgot one important step... we also use a very thin layer of elmer's school glue on the slide for adherence... has some background staining but it's negligible. We also do GMS on these slides. _____ From: Laurie Popp [mailto:Laurie@conxis.com] Sent: Saturday, February 24, 2007 8:21 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] PAS stain for nail fungus We also frequently cut toenails for PAS Staining among others.... we use the liquid dial handsoap from our handwashing area mixed with very little water to make a paste consistency and soak the block in that for about 20 minutes and then cut at our workstations... works like a charm and doesn't affect staining. Laurie Popp HT Candidate, Mayo Clinic From turkekul <@t> gmail.com Sat Feb 24 10:25:50 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Sat Feb 24 10:25:58 2007 Subject: [Histonet] (no subject) Message-ID: From mkaulahao <@t> bellsouth.net Sun Feb 25 05:24:57 2007 From: mkaulahao <@t> bellsouth.net (mkaulahao@bellsouth.net) Date: Sun Feb 25 05:25:07 2007 Subject: [Histonet] Microtome problems Message-ID: <20070225112457.GPR12624.ibm64aec.bellsouth.net@mail.bellsouth.net> Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth From dlcowie <@t> prodigy.net Sun Feb 25 07:32:06 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Sun Feb 25 07:32:47 2007 Subject: [Histonet] Microtome problems In-Reply-To: <20070225112457.GPR12624.ibm64aec.bellsouth.net@mail.bellsouth.net> Message-ID: <748960.32009.qm@web81001.mail.mud.yahoo.com> hi Mary Beth, If you think humidity may be the problem, try placing a piece of warm damp gauze on either side of the microtome (as close to the blade holder as possible). This has worked for me sometimes. You can also try spraying static guard around the blade holder area. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, FL mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 25 09:19:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 25 10:19:37 2007 Subject: [Histonet] Microtome problems In-Reply-To: <20070225112457.GPR12624.ibm64aec.bellsouth.net@mail.bellsouth.net> Message-ID: <20070225151926.72418.qmail@web61217.mail.yahoo.com> How many times your Leitz microtome has been serviced since 1994? I think you should have it serviced (overhawlled) before starting to worry about humidity. If the microtome has some adjustment problems they will be more evident in large blocks than in smaller ones (biopsies) due to less resistance in the smaller ones. Also think that if you are in a new lab your microtome had to be MOVED from the old one, and some disadjustments may hace occurred while moving to the new lab. Have it serviced! Ren? J. mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From sohail_e <@t> yahoo.com Sun Feb 25 12:40:48 2007 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Sun Feb 25 12:40:57 2007 Subject: [Histonet] antibody specific for retinal Message-ID: <20070225184049.72948.qmail@web39509.mail.mud.yahoo.com> Hello everyone I'm looking for an antibody specific for retinal ganglion cells. Does anyone have a source for this antibody? Any info you can give me is most appreciated. Again thanks for any information you can give me. all the best Sohail --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From pamvlies <@t> sbcglobal.net Sun Feb 25 13:08:18 2007 From: pamvlies <@t> sbcglobal.net (Pam V) Date: Sun Feb 25 13:08:28 2007 Subject: [Histonet] Re: Histonet Digest, Vol 39, Issue 41 Message-ID: <60097.40577.qm@web81108.mail.mud.yahoo.com> Hi Mary Beth.. That's a hazard of a well ventilated lab...the ribbons may blow all over the place so it seems you cut everything twice and waste tissue..and cutting thin is a challenge. If blowing on the ribbon as I'm cutting doesn't help, one of my tricks is to use a 50/50 dilution of fabric softener spritzed onto a kimwipe, gauze, tissue or paper towel and place them in the paraffin shavings catcher thingy..but we have Microms which have the waste catcher built in.. Sometimes in utter frustration, I've sprayed and wiped down the entire front of the machine.. Keeping moisture near the blade but not touching it- either with water or the Downey solution - seem to help..most of the techs I work with notice the difference. I compare it to putting a plant on stones and putting water in the stones to provide an area of humidity around the plant which needs the extra moisture but not water in it's root system. It's either THAT or just might could be that the histology gremlins like the smell of the Downey ;-) ALSO, we clean the microtome with oil..that wintergreen smelling stuff? - instead of xylene..I think that makes a difference as well..perhaps the oil helps manage the static a bit.. I've known techs to swear by Static guard and one of the vendors makes an anti static solution that probably costs a fortune and is nothing more than DI water..Another tech I knew wiped the front of the blade holder with absolute alcohol. Another little trick I use to cut thin sections is to use a dilute glycerin/water solution to wet the faced off block with. I try not to soak anything with water. You could try a buffer solution instead of water. Good luck !! Cheers... Pam Vlies HTASCP Evanston Northwestern Healthcare Evanston Il pamvlies@sbcglobal.net ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 25, 2007 11:59:12 AM Subject: Histonet Digest, Vol 39, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Microtome problems (mkaulahao@bellsouth.net) 2. Re: Microtome problems (Dawn Cowie) 3. Re: Microtome problems (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sun, 25 Feb 2007 6:24:57 -0500 From: Subject: [Histonet] Microtome problems To: Message-ID: <20070225112457.GPR12624.ibm64aec.bellsouth.net@mail.bellsouth.net> Content-Type: text/plain; charset=ISO-8859-1 Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth ------------------------------ Message: 2 Date: Sun, 25 Feb 2007 05:32:06 -0800 (PST) From: Dawn Cowie Subject: Re: [Histonet] Microtome problems To: mkaulahao@bellsouth.net, histonet@lists.utsouthwestern.edu Message-ID: <748960.32009.qm@web81001.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 hi Mary Beth, If you think humidity may be the problem, try placing a piece of warm damp gauze on either side of the microtome (as close to the blade holder as possible). This has worked for me sometimes. You can also try spraying static guard around the blade holder area. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, FL mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 25 Feb 2007 07:19:26 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Microtome problems To: mkaulahao@bellsouth.net, histonet@lists.utsouthwestern.edu Message-ID: <20070225151926.72418.qmail@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 How many times your Leitz microtome has been serviced since 1994? I think you should have it serviced (overhawlled) before starting to worry about humidity. If the microtome has some adjustment problems they will be more evident in large blocks than in smaller ones (biopsies) due to less resistance in the smaller ones. Also think that if you are in a new lab your microtome had to be MOVED from the old one, and some disadjustments may hace occurred while moving to the new lab. Have it serviced! Ren? J. mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 41 **************************************** From katherine_dubrall <@t> yahoo.com Sun Feb 25 13:11:33 2007 From: katherine_dubrall <@t> yahoo.com (katherine_dubrall@yahoo.com) Date: Sun Feb 25 13:11:41 2007 Subject: [Histonet] k wants to share sites with you... Message-ID: k (katherine_dubrall@yahoo.com) has invited you to StumbleUpon! You can see my other favorites here: http://erzulieloo.stumbleupon.com Thanks, erzulieloo --- StumbleUpon lets you discover great sites with a single click. Give it a try at: http://www.stumbleupon.com/join.php?friend=2168114&emailcode=6h7fknljvdtty7a4 From valleygal <@t> aol.com Sun Feb 25 13:30:30 2007 From: valleygal <@t> aol.com (valleygal@aol.com) Date: Sun Feb 25 13:30:49 2007 Subject: [Histonet] Microtome problems In-Reply-To: <748960.32009.qm@web81001.mail.mud.yahoo.com> Message-ID: <8C9272A8B405D69-5F0-8421@mblk-r14.sysops.aol.com> In Arizona we deal with dry air much of the year. I have a waste tray that slides in under and around my Microm microtome and I place water soaked gauze or kimwipes in it - not just wet but sopping wet. I don't know for sure but I feel like this helps. I also keep the knife and knife holder clean - brushing it off frequently with a moistened brush. Andi University of Arizona -----Original Message----- From: dlcowie@prodigy.net To: mkaulahao@bellsouth.net; histonet@lists.utsouthwestern.edu Sent: Sun, 25 Feb 2007 6:32 AM Subject: Re: [Histonet] Microtome problems hi Mary Beth, If you think humidity may be the problem, try placing a piece of warm damp gauze on either side of the microtome (as close to the blade holder as possible). This has worked for me sometimes. You can also try spraying static guard around the blade holder area. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, FL mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From pruegg <@t> ihctech.net Sun Feb 25 13:56:14 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Feb 25 13:56:31 2007 Subject: [Histonet] varying time in formalin In-Reply-To: <647525.74774.qm@web61218.mail.yahoo.com> Message-ID: <200702251956.l1PJu8MS073161@pro12.abac.com> Elizabeth, It depends on which marker you want to use, some are more masked by long formalin fixation than others. Most all can be retrieved with enough pretreatment which may require enzyme digestion and HIER methods combined, some are best retrieved with lower heat for longer periods of time, I have done some hier in a waterbath set at 37dc overnight, or a wb at 72dc for 3 hrs., etc. There is one marker (Ki67) that I was not able to demonstrate after 1 yr in formalin, Chris van de Loos did some fixation studies and found that ki67 was lost or not accessible after 3 mos in formalin, most all others I have tried at least have been demonstratable even after 1 yr in formalin. Under fixation is more of a concern for me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, February 24, 2007 7:10 AM To: Stephens, Elizabeth Humes; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] varying time in formalin Elizabeth: As you well know, croslinkage by formalin during fixation (as well as xylene as an antemedium) has cause the development of the Heat Induced Epitope Retrieval (HIER) and, although there is not such a thing as "overfixation", many consider that the longer a tissue is in formalin, the more IHC is "affected". What I would suggest you to do is to get a fresh specimen, fix it for just 6 hours, process it and use it as a positive control against the specimen kept the longest in formalin. Then you will be able to see the difference, if any at all! Just a thoght! Ren? J. "Stephens, Elizabeth Humes" wrote: Hi everyone! I am wondering whether anyone has looked at how much length of time in formalin affects the ability to do immunohistochemistry? For instance, I want to do a pathology study on heart valves but have hearts that have been in formalin 1 year, 2 years, 3 years and am afraid this will affect my results. I would be interested in IHC staining of matrix markers including various collagen types, elastin, fibrillin, proteoglycans, glycoaminoglycans, smooth muscle alpha actin, lysyl oxidase, prolyl 4-hydroxylase. Thank you!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nickandmanda <@t> paradise.net.nz Mon Feb 26 02:47:20 2007 From: nickandmanda <@t> paradise.net.nz (nick bowden) Date: Mon Feb 26 02:47:53 2007 Subject: [Histonet] (no subject) Message-ID: <0JE200JYKB3A2T20@smtp5.clear.net.nz> Hello fellow histonetters. Help!!! We have some strange, random, staining issues!!! we are getting pale patchy staining which seems most noticeable in poc's and mucin type sections, with some obvious uneven or patchy staining which recurs when repeated. SSSooo we are thinking fixation? Processing? The Solutions are all changed on processor and stainer, Haem and Eosin new, and staining control can look fine, only to get more random bad staining. Any ideas would be appreciated.maybe it's the water!! Baffled . From Jacqueline.Malam <@t> rli.mbht.nhs.uk Mon Feb 26 03:00:20 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Mon Feb 26 03:02:14 2007 Subject: [Histonet] RE: Ber EP4 Message-ID: We use the clone Ber EP4 from Lab Vision but it is called Epithelial Specific antigen on their data sheet Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From ales.kladnik <@t> bf.uni-lj.si Mon Feb 26 04:30:15 2007 From: ales.kladnik <@t> bf.uni-lj.si (=?UTF-8?B?QWxlxaEgS2xhZG5paw==?=) Date: Mon Feb 26 04:30:25 2007 Subject: [Histonet] TUNEL stanining - trouble with background Message-ID: <45E2B6B7.4010806@bf.uni-lj.si> Dear Histonetters! For detection of fragmented DNA in tissue sections I'm using TUNEL kit from Roche, with fluorescein labeled nucleotides. The tissue is corn seed, and the problem is that fluorescent nucleotides stick massively to amyloplasts (starch grains). The reaction is not specific, since they don't even contain visible amounts of DNA (determined by DAPI staining). Did anyone of you experienced such a problem with non-specific binding of nucleotides to tissue structures? I've found a reference stating that the problem might be due to calcium in the tissue structures, and the non-specific binding of nucleotides may be abolished by treatment with EDTA or citric acid: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8669464&dopt=Abstract Did anyone try this and willing to share their experience on this? Thank you! -- Ale? Kladnik University of Ljubljana, Biotechnical Faculty, Department of Biology Ve?na pot 111, SI-1000 Ljubljana, Slovenia tel: +386 1 4233388, fax: +386 1 2573390 url: http://botanika.biologija.org/ skype: fridjo, msn: aleskladnik@hotmail.com From abright <@t> brightinstruments.com Mon Feb 26 05:10:49 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Mon Feb 26 05:09:02 2007 Subject: [Histonet] Microtome problems Message-ID: Dear Mary Beth, I agree with Rene's reply email on this, but to be sure remove the cutting knife or blade, cover the specimen clamp and hold firmly with a cloth to eliminate frost bite and in the mid cutting position holding the cutting handwheel too, try to move the specimen clamp from side to side then up and down to see if there is a slight amount of movement in the slideways or bearings. If it is side to side movement the vertical slidways will need adjusting if up and down movement the microtome will need a bearing changed. One other thing to note is that this microtome was fitted in too high a position in the microtome chamber, to over come this design fault they were fitted with a refrigerated specimen holder with many developing leaks due to the constant movement during sectioning, if your ventilation is too close to the opening of the microtome chamber and disturbing the air in this chamber it would also cause sectioning problems. I hope this assist you in overcoming your problem Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: mkaulahao@bellsouth.net [mailto:mkaulahao@bellsouth.net] Sent: 25 February 2007 11:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome problems Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon Feb 26 05:45:29 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Feb 26 05:46:01 2007 Subject: [Histonet] Microtome problems Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3524@sjhaexc02.sjha.org> Gently blowing on the block as you cut also helps provide the right humidity for cutting. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of valleygal@aol.com Sent: Sunday, February 25, 2007 2:31 PM To: dlcowie@prodigy.net; mkaulahao@bellsouth.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome problems In Arizona we deal with dry air much of the year. I have a waste tray that slides in under and around my Microm microtome and I place water soaked gauze or kimwipes in it - not just wet but sopping wet. I don't know for sure but I feel like this helps. I also keep the knife and knife holder clean - brushing it off frequently with a moistened brush. Andi University of Arizona -----Original Message----- From: dlcowie@prodigy.net To: mkaulahao@bellsouth.net; histonet@lists.utsouthwestern.edu Sent: Sun, 25 Feb 2007 6:32 AM Subject: Re: [Histonet] Microtome problems hi Mary Beth, If you think humidity may be the problem, try placing a piece of warm damp gauze on either side of the microtome (as close to the blade holder as possible). This has worked for me sometimes. You can also try spraying static guard around the blade holder area. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, FL mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From RJLevier <@t> LancasterGeneral.org Mon Feb 26 08:58:30 2007 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Mon Feb 26 08:58:43 2007 Subject: [Histonet] Purple Haze Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2019E38E1@MAIL-LR.lha.org> Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JCollins <@t> palmbeachpath.com Mon Feb 26 09:18:35 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Mon Feb 26 09:18:42 2007 Subject: [Histonet] Repeat E-mails Message-ID: Hello all, Am I the only one who is getting the set of 3 E-mails from Paul Monfils, Theresa Rohr and Joyce Reems regarding extended time in paraffin almost hourly? This has been going on for several days now. What is going on? Judy Collins From mcauliff <@t> umdnj.edu Mon Feb 26 09:45:09 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Feb 26 09:44:13 2007 Subject: [Histonet] /patchy staining In-Reply-To: <0JE200JYKB3A2T20@smtp5.clear.net.nz> References: <0JE200JYKB3A2T20@smtp5.clear.net.nz> Message-ID: <45E30085.8010601@umdnj.edu> I suspect that you are not getting all of the paraffin out of the sections before staining. Geoff nick bowden wrote: >Hello fellow histonetters. > > > >Help!!! We have some strange, random, staining issues!!! we are getting >pale patchy staining which seems most noticeable in poc's and mucin type >sections, with some obvious uneven or patchy staining which recurs when >repeated. SSSooo we are thinking fixation? Processing? The Solutions are >all changed on processor and stainer, Haem and Eosin new, and staining >control can look fine, only to get more random bad staining. Any ideas would >be appreciated.maybe it's the water!! > > > >Baffled . > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From ree3 <@t> leicester.ac.uk Mon Feb 26 09:20:40 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Feb 26 09:52:07 2007 Subject: [Histonet] Purple Haze In-Reply-To: <0FDBF29200C637468CCC1F9E7E85A3D2019E38E1@MAIL-LR.lha.org> References: <0FDBF29200C637468CCC1F9E7E85A3D2019E38E1@MAIL-LR.lha.org> Message-ID: Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From danma <@t> reg2.health.nb.ca Mon Feb 26 10:01:29 2007 From: danma <@t> reg2.health.nb.ca (Danahy, Mary-Lee (R2)) Date: Mon Feb 26 10:01:38 2007 Subject: [Histonet] SV40 antibody for Simian Virus Message-ID: <414FA20EC03C3140924A19ACF103D2974C1290@RHAEX2.RHA-RRS.CA> Hi Guys First time at this! Does anybody do any Immunohistochemistry using the SV40 antibody for the Simian virus? If so where do you get the antibody, what is the clone that you use, which pretreatment and dilution do you use, and what do you use for a control ? I would appreciate any help!! Thanks Mary Lee Mary Lee Danahy MLT Immunohistochemistry/Molecular Laboratory Anatomical Pathology Saint John Regional Hospital Phone 506-648-6604 Fax 506-648-6514 danma@reg2.health.nb.ca -------------R2 DISCLAIMER------------- This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any action related with this communication as well as any reproduction, transmission and/or dissemination in whole or in part is strictly prohibited. If you have received this communication in error please notify the sender and delete this email immediately. Thank you. Ce message ?lectronique et tout fichier qui y est joint pourraient contenir des renseignements privil?gi?s ou confidentiels destin?s uniquement ? la personne ou ? l'organisme nomm? ci-dessus. Toute action entreprise relativement ? ce message ainsi que toute reproduction, transmission ou diffusion partielle ou totale de celui-ci sont strictement d?fendues. Si vous avez re?u ce message ?lectronique par erreur, veuillez en informer l'exp?diteur et d?truire le message imm?diatement. Merci. From rjbuesa <@t> yahoo.com Mon Feb 26 10:11:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 26 10:11:55 2007 Subject: [Histonet] (no subject) In-Reply-To: <0JE200JYKB3A2T20@smtp5.clear.net.nz> Message-ID: <145381.57206.qm@web61218.mail.yahoo.com> Check the dewaxing step. Ren? J. nick bowden wrote: Hello fellow histonetters. Help!!! We have some strange, random, staining issues!!! we are getting pale patchy staining which seems most noticeable in poc's and mucin type sections, with some obvious uneven or patchy staining which recurs when repeated. SSSooo we are thinking fixation? Processing? The Solutions are all changed on processor and stainer, Haem and Eosin new, and staining control can look fine, only to get more random bad staining. Any ideas would be appreciated.maybe it's the water!! Baffled . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From turkekul <@t> gmail.com Mon Feb 26 10:22:24 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Mon Feb 26 10:22:36 2007 Subject: [Histonet] purkinje cell staining for processes Message-ID: Hi, I am trying to stain the processes of purkinje cells in paraffin sections of mouse cerebellum. I tried cresyl violet but it stains only the nuclei. Has anybody tried PAS, Golgi stain or some impregnation methods? I am open to suggestions. Regards, Mesruh Turkekul Molecular Cytology Memorial Slaon-Kettering Cancer Center New York, NY 10021 646-888-2209 From LewisS <@t> pediatrics.ohio-state.edu Mon Feb 26 10:46:10 2007 From: LewisS <@t> pediatrics.ohio-state.edu (Lewis, Sarah) Date: Mon Feb 26 10:46:40 2007 Subject: [Histonet] Muscle lab positions available Message-ID: <714B9F12B4E18C4C843B66E8E190F2AD01340ABE@res2k3ms01.CRII.ORG> We currently have a position available at Columbus Childrens Hospital in the Neuromuscular Lab. We specialize in muscle and nerve processing. The person we are looking for will be assisting in the lab for clinical histology needs (Frozen sections, Immunohistochemistry, Enzyme histochemistry and some basic histology). This person will also be responsible for specialized assays specific for muscle proteins. This position will come with extensive training, and requires someone with a scientific background. We also have an Research assistant/animal technician position available. The person will assist in multiple research projects, involving gene therapy applications. The person will be responsible for running basic and molecular biology experiments. They will also assist with research animal surgeries and housing. We have a wonderful working environment here, with lots of exciting clinical diagnostics and research going on. If you are interested in either of these positions please contact Chris Shilling at ShillinC@ccri.net . Sarah E. Lewis Histotechnician Childrens Research Center for Gene Therapy 700 Childrens Dr Rm WA3112 Columbus OH 43205 (614)-722-2204 LewisS@ccri.net From wqi <@t> biocare.net Mon Feb 26 10:57:48 2007 From: wqi <@t> biocare.net (Weimin Qi) Date: Mon Feb 26 10:57:18 2007 Subject: [Histonet] unsubscribe Message-ID: <20070226165709.TJXU2180.imta02a2.registeredsite.com@Wqi> Please remove me from the list. Thanks, Weimin Qi From rjbuesa <@t> yahoo.com Mon Feb 26 11:27:12 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 26 11:27:21 2007 Subject: [Histonet] purkinje cell staining for processes In-Reply-To: Message-ID: <416562.48222.qm@web61217.mail.yahoo.com> Use Bielchowski's Ren? J. mesruh turkekul wrote: Hi, I am trying to stain the processes of purkinje cells in paraffin sections of mouse cerebellum. I tried cresyl violet but it stains only the nuclei. Has anybody tried PAS, Golgi stain or some impregnation methods? I am open to suggestions. Regards, Mesruh Turkekul Molecular Cytology Memorial Slaon-Kettering Cancer Center New York, NY 10021 646-888-2209 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From fhansen <@t> ventanamed.com Mon Feb 26 12:08:49 2007 From: fhansen <@t> ventanamed.com (Frances Hansen) Date: Mon Feb 26 12:08:58 2007 Subject: [Histonet] Surgipath Auto Glass Coverslipper Message-ID: <4F9A1711C1095543B652EC7EEEC157D0146E124B@BLUEMAX.VENTANA.VENTANAMED.COM> I was just wondering if anyone had any feedback on Surgipath glass coverslipper, we were interested in purchasing this year. Any comments would be helpful, the good or the bad. Thanks Frances Hansen From ASenn <@t> mercy.pmhs.org Mon Feb 26 12:34:38 2007 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Mon Feb 26 12:34:55 2007 Subject: [Histonet] unsubscribe Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61AB4@pmhs2kxch03.pmhs.org> Unsubscribe please Thanks, I'm re-subscribing with my home email! :) Amy R. Senn, Surgical Pathology The Mercy Hospital of Pittsburgh 1400 Locust Street Pittsburgh, PA 15219-5166 412-232-7847 ASenn@mercy.pmhs.org Heart Disease is the leading cause of death among men & women in the U.S. This month-American Heart Month-the physicians at Mercys Heart Institute ask you to be more active in your heart health. Call for more info 1-800-232-5660 www.mercylink.org From rjbuesa <@t> yahoo.com Mon Feb 26 12:36:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 26 12:36:54 2007 Subject: [Histonet] Surgipath Auto Glass Coverslipper In-Reply-To: <4F9A1711C1095543B652EC7EEEC157D0146E124B@BLUEMAX.VENTANA.VENTANAMED.COM> Message-ID: <45986.94184.qm@web61225.mail.yahoo.com> Would you consider to try out either the Sakura or the Leica as well? Both are quite reliable. Ren? J. Frances Hansen wrote: I was just wondering if anyone had any feedback on Surgipath glass coverslipper, we were interested in purchasing this year. Any comments would be helpful, the good or the bad. Thanks Frances Hansen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From JKOBLER <@t> PARTNERS.ORG Mon Feb 26 12:43:50 2007 From: JKOBLER <@t> PARTNERS.ORG (Kobler, James) Date: Mon Feb 26 12:45:42 2007 Subject: [Histonet] Looking to equip an histology lab In-Reply-To: <20070226180212.B10C922D549@phsmgmx6.partners.org> Message-ID: <6D1DFC2837CEAE4BBBDED59607143085156CF8@PHSXMB4.partners.org> Hello - I'm am looking to outfit a new histology lab and am looking for a Leica 2245 (or older similar model) and a cryostat such as the Leica CM1850. I also need a complement of staining dishes and slide racks for manual staining. I would appreciate any advice about where I might find a good deal on such items. Thanks, Jim Kobler Mass General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From amylee779 <@t> yahoo.com Mon Feb 26 12:47:56 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Feb 26 12:48:04 2007 Subject: [Histonet] block/slides storage Message-ID: <935308.36729.qm@web38011.mail.mud.yahoo.com> Hello histonetters, I am looking for a block/slides storage system. I work in industry. Could anybody recommend a vender? Thanks, Amy --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From rjbuesa <@t> yahoo.com Mon Feb 26 12:53:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 26 12:53:08 2007 Subject: [Histonet] Looking to equip an histology lab In-Reply-To: <6D1DFC2837CEAE4BBBDED59607143085156CF8@PHSXMB4.partners.org> Message-ID: <900926.99258.qm@web61225.mail.yahoo.com> There are some vendors of refurbished instruments in EBay. Ren? J. "Kobler, James" wrote: Hello - I'm am looking to outfit a new histology lab and am looking for a Leica 2245 (or older similar model) and a cryostat such as the Leica CM1850. I also need a complement of staining dishes and slide racks for manual staining. I would appreciate any advice about where I might find a good deal on such items. Thanks, Jim Kobler Mass General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From MLashus <@t> pathgroup.com Mon Feb 26 13:02:35 2007 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Mon Feb 26 13:02:43 2007 Subject: [Histonet] Grossing Specimens Message-ID: What are the education requirements for grossing specimens? CLIA and CAP? Thanks, Mighnon Lashus Laboratory Manager PathGroup Lab Chattanooga, TN --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From gu.lang <@t> gmx.at Mon Feb 26 13:12:00 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Feb 26 13:11:56 2007 Subject: [Histonet] forensic histotechniques? Message-ID: <000401c759d9$fe61b520$6412a8c0@dielangs.at> Hi, I am interested in the differences between surgical and forensic histotechnology. What are the main indications for a histo in this field? What are the main staining methods that are used? What are the difficulties of handling this tissue? Thanks Gudrun Lang From staceylburton <@t> yahoo.com Mon Feb 26 13:27:40 2007 From: staceylburton <@t> yahoo.com (Stacey Burton) Date: Mon Feb 26 13:27:54 2007 Subject: [Histonet] staining problems Message-ID: <590591.84933.qm@web30010.mail.mud.yahoo.com> Nick, I agree with the advise given so far. Your problem is most likely in the "deparaffinization" process. Try changing out and using fresh xylene - maybe bump the time up a little also by one - two minutes. * Are you using a xylene substitute? If so, this could also be your problem - try changing back to good old xylene for paraffin removal on your slides for IHC staining - 4 changes @ 3 - minutes each. -Stacey ____________________________________________________________________________________ TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. http://tv.yahoo.com/ From Luis.Chiriboga <@t> med.nyu.edu Mon Feb 26 13:54:11 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Mon Feb 26 13:49:02 2007 Subject: [Histonet] New York State License Message-ID: Dear Fellow Histonetters As you may or may not know; in 2006, New York State passed the Clinical Laboratory Technology Act (Chapter 755 of the laws of 2004, Article 165). The law requires that all laboratory personnel in New York State be licensed in order to practice. However, the legislation did not take into account the unique educational requirements of histotechnology or recognize histotechnology as a separate entity with in laboratory medicine. As a result, the law has no provisions for the education of histotechnologist. Clearly, this will only serve to intensify the current shortages and increase the burden of already overworked staff. The New York State Histotechnology Society is extremely concerned with the impact that this legislation will have on the histology profession and patient care in New York State. The society has created a task force to address this issue (additional information is available on the NYSHS website http://www.nyhisto.org/ ). We are actively engaged in discussions with the legislation to modify the current language. However, we need your support in order for our voice to be heard in the legislature. In order to facilitate communication and keep individuals in New York State informed, the Society has created a message board to keep everyone up to date and allow concerns, suggestions, questions and answers to be posted regarding licensing (and any other issue). The message board is open to all interested individuals (licensed or not). To join the website, please visits: http://tech.groups.yahoo.com/group/NYSHS1972/ For security reasons, you will be required to create a yahoo ID. You do not have to subscribe to Yahoo services. Creating the ID will allow you to visit all pages within the group website as well as modify your user preferences. Alternatively, you can send a message to: NYSHS1972-subscribe@yahoogroups.com If you have any questions or difficulty subscribing or logging on, please contact the list-owner by sending a message to: NYSHS1972-owner@yahoogroups.com You can also contact the owner directly at: litepath2000@yahoo.com Thanks and regards to all Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 From TJasper <@t> smdc.org Mon Feb 26 14:29:15 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Mon Feb 26 14:29:29 2007 Subject: [Histonet] LIS programs In-Reply-To: <45DEBC7B0200003C00006365@gwia.alegent.org> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A53A@SCREECH.ntcampus.smdc.org> This is interesting, we have Tamtron (PowerPath) and like it very much. Does anyone have an opinion about SoftPath? Thanks, TomJ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Janice Mahoney Sent: Friday, February 23, 2007 10:06 AM To: histonet@lists.utsouthwestern.edu; Marilyn McDonald; Joyce Weems Subject: RE: [Histonet] LIS programs We have Cerner millennium and I absolutely love it. If CoPath is the Mercedes Cerner is the Maserati, in my opinion. Please feel free to call me if you want to know details. Jan Omaha 402-717-2889 >>> "Weems, Joyce" 02/23/2007 5:24 AM >>> Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP programs. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From plucas <@t> biopath.org Mon Feb 26 14:38:12 2007 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon Feb 26 14:38:25 2007 Subject: [Histonet] Job Opportunity in Southern California Message-ID: <20070226203812.57CBC2344@courageux.cnchost.com> Attention- We will have an opening for a Histotech in our laboratory. We are located in Fountain Valley, California. We've been in business for 28 years and we serve Fountain Valley Hospital, Bellflower Medical Center and surrounding Surgery Centers. If interested, please let me know and I will give you more detailed information. Paula Lucas Lab Manager Bio-Path Medical Group From gtesdall <@t> yahoo.com Mon Feb 26 14:50:32 2007 From: gtesdall <@t> yahoo.com (greg tesdall) Date: Mon Feb 26 14:50:41 2007 Subject: [Histonet] Cryocut 1800 accessories Message-ID: <638369.11671.qm@web33512.mail.mud.yahoo.com> I'm looking to purchase 30mm specimen discs for a Leica Cryocut 1800 cryostat. Can anyone direct me to a vender. Thanks, Greg --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From splaza <@t> tylerpathology.com Mon Feb 26 15:15:43 2007 From: splaza <@t> tylerpathology.com (Susan Plaza) Date: Mon Feb 26 15:26:35 2007 Subject: [Histonet] RE: Removing "build up" from reagent containers In-Reply-To: <200702211641.l1LGfPLN020577@inbound-mx46.atl.registeredsite.com> Message-ID: <001201c759eb$46ee08a0$800aa8c0@domain.local> We remove lithium carbonate build up from our frozen room containers with vinegar. It works wonders when you do not have time to soak over night. As for the 70% alchol, I'm not sure. Good luck. Susan Plaza, HT(ASCP), QIHC Pathology Associates of Tyler Tyler, TX Message: 18 Date: Wed, 21 Feb 2007 07:30:25 -0500 From: "sheila adey" Subject: [Histonet] Removing "build up" from reagent containers To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Hi Histonetters, We get a "build up" on the edges of our 70% alcohol container on our stainer but no "build up" on the 70% alcohol container on the processor. (we use tap water to make up the 70%) The 1% Lithium Carbonate that we use for bluing in the frozen room also develops this "build up"? We've tried so many ways to clean these containers but have not found an effective solution. Any advice would be greatly appreciated. Have a great day!! Sheila Adey HT MLT Port Huron Hospital Michigan From hawkmoon15 <@t> cox.net Mon Feb 26 15:36:14 2007 From: hawkmoon15 <@t> cox.net (Sarah Jones) Date: Mon Feb 26 15:37:10 2007 Subject: [Histonet] Questions for John Kiernan Message-ID: <20070226213702.DTLS6078.fed1rmmtao101.cox.net@fed1rmimpo02.cox.net> Hello John, I have a couple of questions for you, if you would be so kind as to answer: 1) Is there a problem with the Alcian Blue that's on the market today? We are having problems with the acid mucin being masked by the hematoxylin. I am aware that some hematoxylins do stain mucin, but this just doesn't look like the AB/PAS-H I have been used to seeing over the years. 2) Is there a treatment for aspergillus that will cause it not to stain with Schiff's? I have used aspergillus for a PAS fungus control for years, and have never seen this problem before. It stains fine with a GMS. Thank you for any help you can give me. I have your book Histological and Histochemical Methods Theory & Practice and have not found the answer to my fungus question in it, although you explain about Alcian Blue and I found reading that helpful. Thank you again! Sarah A. Jones From relia1 <@t> earthlink.net Mon Feb 26 15:51:06 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Feb 26 15:51:16 2007 Subject: [Histonet] RELIA's Histology Careers Bulletin from Pam Barker 2-26-07 Message-ID: Hello Histonetters, Timing is Everything Sometimes your next opportunity is just a matter of being in the right place at the right time. That is what we can do for you at RELIA. Stay in touch with RELIA and stay in touch with what is going on in the histology job market. Remember we offer Resume help, Career coaching and Personalized Job Searches. All of the permanent positions that I represent are with great companies that provide excellent compensation benefits and relocation assistance. These positions are day shift and fulltime unless otherwise indicated. Here is a list of the current opportunities I can show you. ***Permanent*** Histo Tech ? Illinois ? positions statewide Histo Tech ? Central New Hampshire IHC support and training Tech ? Dallas/Houston Histo Tech ? Washington D.C. Histology Supervisor ? Boston *Histology Supervisor ? Central Florida *Histo Tech ? Northern Central Florida *Histo Tech/PA ? West Central Florida *Histo Tech ? Southwestern Florida. *Note on Florida Licensing, In our experience Florida licensing takes about 6 weeks to process if your application is filled out properly and we can help you with that. ***Travel/Temp*** Travel tech openings are available in: Dallas Boston Kansas Ohio Remember if nothing sounds interesting on my current list you can pass it on to your friends and wait for the next one OR give me a call or shoot me an e-mail telling me what you are looking for in your next opportunity. Remember timing is everything. Hope to hear from you soon . Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From rchiovetti <@t> yahoo.com Mon Feb 26 17:09:46 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Mon Feb 26 17:09:55 2007 Subject: [Histonet] Cryocut 1800 accessories Message-ID: <92676.70030.qm@web58901.mail.re1.yahoo.com> Greg, I can't tell where you're located from your email address, so contact Leica Customer Service Directly at: 800-248-0123 Best, Bob Chiovetti Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Tucson, AZ Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: greg tesdall To: histonet@lists.utsouthwestern.edu Sent: Monday, February 26, 2007 1:50:32 PM Subject: [Histonet] Cryocut 1800 accessories I'm looking to purchase 30mm specimen discs for a Leica Cryocut 1800 cryostat. Can anyone direct me to a vender. Thanks, Greg --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/features_spam.html From barbara <@t> ponath.com Mon Feb 26 17:14:57 2007 From: barbara <@t> ponath.com (Barbara Ponath) Date: Mon Feb 26 17:15:36 2007 Subject: [Histonet] Unsubscribe Message-ID: <0JE300MURF8XWEK9@vms115.mailsrvcs.net> From jnocito <@t> satx.rr.com Mon Feb 26 17:39:53 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Feb 26 17:40:04 2007 Subject: [Histonet] TSH meeting schedule Message-ID: <000b01c759ff$6a8ce6a0$649eae18@yourxhtr8hvc4p> I know some of you have been asking about the Texas meeting. The program is now on our website at www.txsh.org If you go to www.tsh.org, you'll get a whole bunch of information about the thyroid, which is ok if you want info on the thyroid, but if you want info on the Texas meeting, you need to put in that pesky "x". Hope to see a whole bunch in April JTT (Joe the Toe) From jnocito <@t> satx.rr.com Mon Feb 26 17:57:40 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Feb 26 17:57:55 2007 Subject: [Histonet] Purple Haze References: <0FDBF29200C637468CCC1F9E7E85A3D2019E38E1@MAIL-LR.lha.org> Message-ID: <007401c75a01$e6bc8490$649eae18@yourxhtr8hvc4p> is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon Feb 26 20:26:01 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Feb 26 20:26:35 2007 Subject: [Histonet] Purple Haze Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3559@sjhaexc02.sjha.org> Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Feb 27 01:58:36 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Feb 27 01:58:49 2007 Subject: [Histonet] Purple Haze Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012472FE@wahtntex2.waht.swest.nhs.uk> Yes I agree, purple haze occurs either with 'badly blued' H&Es or when using 'overoxidised' haematoxylin. Fresh Haematoxylin and running water of adequate pressure usually eradicates the problem with the latter being patchy in distribution. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com Science is organized knowledge. Wisdom is organized life. --Immanuel Kant This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Feb 27 02:13:38 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Feb 27 02:13:48 2007 Subject: [Histonet] /patchy staining Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247302@wahtntex2.waht.swest.nhs.uk> I suspect that you are not getting all of the paraffin out of the sections before staining. Geoff That's one solution but I would have thought unlikely as you would notice it wouldn't you? I've seen similar occurrences with poor fixation whereby staining has been patchy (red in areas, blue in others). Sometimes disappears with restaining but that could be a function of actually removing the paraffin as you say or there may even be a link to the staining problem also being discussed (bluing water not bluing). Does the problem disappear if the sections are restained? If it does I'd go for the paraffin, if not I'd go for fixation. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com Science is organized knowledge. Wisdom is organized life. --Immanuel Kant This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ree3 <@t> leicester.ac.uk Tue Feb 27 03:09:22 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Feb 27 03:09:37 2007 Subject: [Histonet] Purple Haze In-Reply-To: <007401c75a01$e6bc8490$649eae18@yourxhtr8hvc4p> References: <007401c75a01$e6bc8490$649eae18@yourxhtr8hvc4p> Message-ID: Well it was in my youth, Cardiff 1966, supporting the Walker Brothers, those were the days!. -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: 26 February 2007 23:58 To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Feb 27 04:23:25 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Feb 27 04:23:33 2007 Subject: [Histonet] smad 6 and smad7 Message-ID: Hi all, I'm just going through a spine chilling moment. After ordering the above antibodies for IHC, I read the data sheet only to see that they are recommended for immunofluorescence and western blotting. Is there any way I can retrieve the situation? Is it worth trying to optimise or is this a lost cause? Failing that - can anybody recommend a source of smad 6 and smad7 antibodies for immuno in FFPE tissue? Thank you in advance for (hopefully) saving my bacon (apologies to kosher & halaal friends) -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Eric <@t> ategra.com Mon Feb 26 14:58:07 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Tue Feb 27 05:43:32 2007 Subject: [Histonet] Revised - (2/26/07) - Current Histology Temporary/Travel Assignments - Should I call you to discuss ? Message-ID: Hi, Histonetters - I am presently on a search for a couple of my best client companies who are seeking to hire Travel Histotechs. Sorry for the duplicate email, I had to add a new positon in Southeast Florida. I have two HistoTech Temporary/Travel assignments open: 1. HistoTech Travel Assignment #1 - Western PA - State of the art lab - 3-6 month assignment 2. HistoTech Travel Assignment #2 - Central Virginia - 3 month assignment - State of the art lab 3. HistoTech Travel Assignment #3 - Southeast Coastal Florida Central Virginia - 6 weeks assignment 4. Other HistoTech Travel Assignments - various locations are opening up soon - please call me to discuss. Are you interested ? Should I call you to discuss, or would you rather call me ? What is the best number to reach you ? We provide all housing, meals, per-diem and travel exp, and of course a very high hourly rate ! If you would you rather call me, please call me at: 800-466-9919 ext223 (office) - Eric Dye, Senior Recruiter, PH: 800-466-9919 ext 223, CELL: 407-756-5507 PS: Because of the deadlines, Please call me ASAP to get you started. --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing PHONE: 800-466-9919 ext 223 FAX: 407-671-6075 To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu -------------------------------------------------------------------------------------- From lblazek <@t> digestivespecialists.com Tue Feb 27 06:18:35 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 27 06:19:01 2007 Subject: [Histonet] Purple Haze References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3559@sjhaexc02.sjha.org> Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684D55@bruexchange.digestivespecialists.com> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Tue Feb 27 07:11:46 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Tue Feb 27 07:13:44 2007 Subject: [Histonet] Purple Haze Message-ID: How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lelmgren <@t> sunriselab.com Tue Feb 27 08:52:17 2007 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Tue Feb 27 08:54:06 2007 Subject: [Histonet] Thermo Shandon Laser Slide Labeller Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E321C4B7@MailPDC.sunriselab.com> Has anyone had any experience, comments about the Thermo Shandon Laser Slide Labeller? We would like to purchase it for use in a cyto and pathology lab setting. Very pricey, is it worth it? Laurie From settembr <@t> umdnj.edu Tue Feb 27 09:58:34 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Feb 27 09:59:34 2007 Subject: [Histonet] PMS2 for IVD Message-ID: Hello, I am looking for PMS2, colon cancer screening marker for in vitro diagnostic use on FFPE human tissue. Is there any? I've looking and looking. Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From mc549 <@t> hotmail.com Tue Feb 27 10:23:46 2007 From: mc549 <@t> hotmail.com (M C) Date: Tue Feb 27 10:24:00 2007 Subject: [Histonet] Histology Technicians Needed Message-ID: ***Open Positions for Laboratory Technicians available in New Histology Lab located in Southern California. Licensing not necessary. For more details, please reply to e-mail.*** -Marco Corona _________________________________________________________________ With tax season right around the corner, make sure to follow these few simple tips. http://articles.moneycentral.msn.com/Taxes/PreparationTips/PreparationTips.aspx?icid=HMFebtagline From histologyfield <@t> yahoo.com Tue Feb 27 10:30:10 2007 From: histologyfield <@t> yahoo.com (Histology Field) Date: Tue Feb 27 10:30:20 2007 Subject: [Histonet] please unsubscribe Message-ID: <781988.39440.qm@web59201.mail.re1.yahoo.com> --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From mc549 <@t> hotmail.com Tue Feb 27 10:42:41 2007 From: mc549 <@t> hotmail.com (M C) Date: Tue Feb 27 10:42:55 2007 Subject: [Histonet] Anyone need some help... Message-ID: If anyone needs some help finding these pieces of equipment: Linistat H&E Stainer E300 DRS-601 VIP2 Microm HM 355 Microtome I have them. Reply to my post for more details. Thanks, Marco _________________________________________________________________ With tax season right around the corner, make sure to follow these few simple tips. http://articles.moneycentral.msn.com/Taxes/PreparationTips/PreparationTips.aspx?icid=HMFebtagline From Crystalmo <@t> fmchealth.org Tue Feb 27 10:59:54 2007 From: Crystalmo <@t> fmchealth.org (Crystal Morris) Date: Tue Feb 27 11:00:01 2007 Subject: [Histonet] Blue Feulgen Stain Message-ID: <8F60E26ED772F54C8B1D39AAEC6ADA4901C34CF7@ex03.fmchealth.org> Is there anyone who is currently doing the blue feulgen stain and scoring it on the Chromavision Acis or an equivalent imaging system? If so can you tell me how this is going for your facility and in what capacity are you using it? Our pathologists want to use it for the bronchial biopsies that are going to be collected using an UV light endoscope. Is anyone doing this? Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From FUNKM <@t> mercyhealth.com Tue Feb 27 11:29:41 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Feb 27 11:30:16 2007 Subject: [Histonet] Dispensable knife Message-ID: <45E4162502000016003EB537@nodcmsngwia1.trinity-health.org> Howdy, I would like to look at different microtome blades. We are using Accu-Edge Low Profile and they seem a bit pricy. I would like info on what types your lab is using . From jmahoney <@t> alegent.org Tue Feb 27 11:45:33 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Tue Feb 27 11:45:57 2007 Subject: [Histonet] Purple Haze In-Reply-To: References: Message-ID: <45E419DD0200003C00006841@gwia.alegent.org> Ah, How about the 60's. I can tell some of you younger people don't know the difference between Purple Haze and Purple Rain. Purple Haze is indeed Jimmy and I'm sure the cause of Purple Haze in those days was LSD not paraffin. >>> "Fred Underwood" 02/27/2007 7:11 AM >>> How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Feb 27 11:49:05 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Feb 27 11:49:35 2007 Subject: [Histonet] Purple Haze Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D356D@sjhaexc02.sjha.org> oh my goodness... I am one of those... Joe the Toe.. I apologize. I should know better but I am too young.....j -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Tuesday, February 27, 2007 12:46 PM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; Fred Underwood; jnocito@satx.rr.com; Weems, Joyce Subject: RE: [Histonet] Purple Haze Ah, How about the 60's. I can tell some of you younger people don't know the difference between Purple Haze and Purple Rain. Purple Haze is indeed Jimmy and I'm sure the cause of Purple Haze in those days was LSD not paraffin. >>> "Fred Underwood" 02/27/2007 7:11 AM >>> How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From tkngflght <@t> yahoo.com Tue Feb 27 11:58:20 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Tue Feb 27 11:58:31 2007 Subject: [Histonet] Dispensable knife In-Reply-To: <45E4162502000016003EB537@nodcmsngwia1.trinity-health.org> References: <45E4162502000016003EB537@nodcmsngwia1.trinity-health.org> Message-ID: <001201c75a98$df189ed0$6401a8c0@FSDESKTOP> While I don't have a list of other blade brands for you-- I do have a few things to add to your list when considering cost of blades in general. Have you calculated how many slides or blocks your techs produce per box of blades? AccuEdge blades do seem to be pricey but I've always gone through more of most of the other kinds...price per slide was uniformly better with low-profile AccuEdge and a thicker one like Sturkey for bone and hard tissue. Also consider the frustration factor. I don't care HOW much the blades cost when I'm trying to get 40 slides off a hair-thin prostate biopsy! In fairness, it takes a couple of days with a new blade to get used to the differences. Having both types around during a new blade trial reduces the fear factor and keeps people's minds a little more receptive to the changes. Some labs stock three or four different types--each tech picks what works for them and is responsible to keep the order clerk informed of when they need more. My two cents :-) Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, February 27, 2007 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dispensable knife Howdy, I would like to look at different microtome blades. We are using Accu-Edge Low Profile and they seem a bit pricy. I would like info on what types your lab is using . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Feb 27 12:11:42 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Feb 27 12:10:28 2007 Subject: [Histonet] Purple Haze In-Reply-To: Message-ID: Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atebo <@t> aahs.org Tue Feb 27 13:09:19 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Tue Feb 27 13:09:38 2007 Subject: [Histonet] Her2 Guidelines Message-ID: <00790F10D0600A41AEE8899901E533A61044B4FB@aamcexch.aamc.org> I am not sure how many of you get the Archives of Pathology and Laboratory Medicine, but there is an article in the January 2007 edition which states that "Fixation times for needle biopsies has not been addressed". It is on page 38 under Appendix E. With this statement, if we are doing Her2 on breast core biopsies, nothing needs to change and the guideline would only pertain to specimens which require sectioning in the grossing stage. What do you think? Curious, Andrea Tebo Supervisor Pathology Anne Arundel Medical Center 443-481-4240 ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From jflinn <@t> gmu.edu Tue Feb 27 13:37:18 2007 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Feb 27 13:39:56 2007 Subject: [Histonet] Dispensable knife In-Reply-To: <001201c75a98$df189ed0$6401a8c0@FSDESKTOP> References: <45E4162502000016003EB537@nodcmsngwia1.trinity-health.org> <001201c75a98$df189ed0$6401a8c0@FSDESKTOP> Message-ID: What type of disposable blades for a cryostat would you recommend? We are slicing mouse brains. Leica high profile have been suggested. jane Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Cheryl Kerry Date: Tuesday, February 27, 2007 12:58 pm Subject: RE: [Histonet] Dispensable knife > While I don't have a list of other blade brands for you-- I do > have a few > things to add to your list when considering cost of blades in general. > > > > Have you calculated how many slides or blocks your techs produce > per box of > blades? AccuEdge blades do seem to be pricey but I've always gone > throughmore of most of the other kinds...price per slide was > uniformly better with > low-profile AccuEdge and a thicker one like Sturkey for bone and hard > tissue. > > > > Also consider the frustration factor. I don't care HOW much the > blades cost > when I'm trying to get 40 slides off a hair-thin prostate biopsy! In > fairness, it takes a couple of days with a new blade to get used > to the > differences. Having both types around during a new blade trial > reduces the > fear factor and keeps people's minds a little more receptive to > the changes. > > > > Some labs stock three or four different types--each tech picks > what works > for them and is responsible to keep the order clerk informed of > when they > need more. > > > > My two cents :-) > > > > Cheryl > > > > Cheryl Kerry, HT(ASCP) > > Full Staff Inc. > > 281.852.9457 > > 800.756.3309 > > Sign up for the FREE newsletter AP News--updates, tricks of the > trade and > current issues for Anatomic Pathology Clinical Labs. Send a > 'subscribe'request to APNews@fullstaff.org. Please include your > name and specialty in > the body of the email. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marcia Funk > Sent: Tuesday, February 27, 2007 11:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dispensable knife > > > > Howdy, > > > > I would like to look at different microtome blades. We are using > Accu-Edge > Low Profile and they > > seem a bit pricy. I would like info on what types your lab is > using . > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Tue Feb 27 13:49:58 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 27 13:50:11 2007 Subject: [Histonet] Dispensable knife In-Reply-To: Message-ID: <147041.26110.qm@web61214.mail.yahoo.com> A tough large high profile disposable blade is always better: it will cool better and will maintain the low temperature better. Ren? J. Jane M Flinn wrote: What type of disposable blades for a cryostat would you recommend? We are slicing mouse brains. Leica high profile have been suggested. jane Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Cheryl Kerry Date: Tuesday, February 27, 2007 12:58 pm Subject: RE: [Histonet] Dispensable knife > While I don't have a list of other blade brands for you-- I do > have a few > things to add to your list when considering cost of blades in general. > > > > Have you calculated how many slides or blocks your techs produce > per box of > blades? AccuEdge blades do seem to be pricey but I've always gone > throughmore of most of the other kinds...price per slide was > uniformly better with > low-profile AccuEdge and a thicker one like Sturkey for bone and hard > tissue. > > > > Also consider the frustration factor. I don't care HOW much the > blades cost > when I'm trying to get 40 slides off a hair-thin prostate biopsy! In > fairness, it takes a couple of days with a new blade to get used > to the > differences. Having both types around during a new blade trial > reduces the > fear factor and keeps people's minds a little more receptive to > the changes. > > > > Some labs stock three or four different types--each tech picks > what works > for them and is responsible to keep the order clerk informed of > when they > need more. > > > > My two cents :-) > > > > Cheryl > > > > Cheryl Kerry, HT(ASCP) > > Full Staff Inc. > > 281.852.9457 > > 800.756.3309 > > Sign up for the FREE newsletter AP News--updates, tricks of the > trade and > current issues for Anatomic Pathology Clinical Labs. Send a > 'subscribe'request to APNews@fullstaff.org. Please include your > name and specialty in > the body of the email. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marcia Funk > Sent: Tuesday, February 27, 2007 11:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dispensable knife > > > > Howdy, > > > > I would like to look at different microtome blades. We are using > Accu-Edge > Low Profile and they > > seem a bit pricy. I would like info on what types your lab is > using . > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From ftulenko06 <@t> jcu.edu Tue Feb 27 14:15:03 2007 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Tue Feb 27 14:15:14 2007 Subject: [Histonet] serial sections Message-ID: <20070227151503.ACL64347@mirapoint.jcu.edu> Dear all, I have been trying to use serial sections (generated from paraplast-embedded chick embryos) to make three dimensional reconstructions of various skeletal structures. The problem I encounter is that the sections seem to move on the slides during drying (thus making smooth reconstructions impossible). Does anyone have advice on how to minimize section movement on slides? The slides I am using are Fisherbrand superfrost/Plus slides. Thank you for your times and suggestions. Sincerely, Frank From Tiffany.Price <@t> thomaswv.org Tue Feb 27 14:18:05 2007 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Tue Feb 27 14:18:06 2007 Subject: [Histonet] RE: microtome problems In-Reply-To: <20070225180328.1D39E1348055@mail15-dub.bigfish.com> Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C32188@thm-mail.thomaswv.org> I had the same problem, and we had our Leica microtome serviced- the block holder was not holding the blocks tight enough, and when the service tech replaced it, problem solved. Tiffany -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, February 25, 2007 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 39, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Microtome problems (mkaulahao@bellsouth.net) 2. Re: Microtome problems (Dawn Cowie) 3. Re: Microtome problems (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sun, 25 Feb 2007 6:24:57 -0500 From: Subject: [Histonet] Microtome problems To: Message-ID: <20070225112457.GPR12624.ibm64aec.bellsouth.net@mail.bellsouth.net> Content-Type: text/plain; charset=ISO-8859-1 Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth ------------------------------ Message: 2 Date: Sun, 25 Feb 2007 05:32:06 -0800 (PST) From: Dawn Cowie Subject: Re: [Histonet] Microtome problems To: mkaulahao@bellsouth.net, histonet@lists.utsouthwestern.edu Message-ID: <748960.32009.qm@web81001.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 hi Mary Beth, If you think humidity may be the problem, try placing a piece of warm damp gauze on either side of the microtome (as close to the blade holder as possible). This has worked for me sometimes. You can also try spraying static guard around the blade holder area. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, FL mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 25 Feb 2007 07:19:26 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Microtome problems To: mkaulahao@bellsouth.net, histonet@lists.utsouthwestern.edu Message-ID: <20070225151926.72418.qmail@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 How many times your Leitz microtome has been serviced since 1994? I think you should have it serviced (overhawlled) before starting to worry about humidity. If the microtome has some adjustment problems they will be more evident in large blocks than in smaller ones (biopsies) due to less resistance in the smaller ones. Also think that if you are in a new lab your microtome had to be MOVED from the old one, and some disadjustments may hace occurred while moving to the new lab. Have it serviced! Ren? J. mkaulahao@bellsouth.net wrote: Hi everyone, This is my first message. I just found this site and hope anyone can help..and I'm a histotech NOT a speller. We moved into a new lab in October 2006. It is really nice compared to our old one. The ventalation is great. There is no fume smell at all. Also there is no humidity. I have NEVER had so much trouble cutting. We got a humidifier but it doesn't seem to do any good. I can't get a ribbon to form but only on bigger specimens. The biopsies (gi,bladder etc) seem to cut ok. Now suddenly the pathologist is asking for thinner sections. He is convinced we need a new mictotome that ours is not cutting right. We have a Lietz and have had it since 1994, We cut on 4um. It takes us twice as long to cut as it ever has. I have spoken to the engineers and they say they can't put any moisture in the air. How can the biopsies cut ok but the big specimens be too thick? Mary Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 41 **************************************** Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From liz <@t> premierlab.com Tue Feb 27 14:35:23 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Feb 27 14:27:40 2007 Subject: [Histonet] serial sections In-Reply-To: <20070227151503.ACL64347@mirapoint.jcu.edu> Message-ID: <000001c75aae$ce0c9950$0d00a8c0@domain.Premier> Frank We have done 3D construction in the past with mouse aortas in Image Pro Plus, when we first did it we had to manually line up the images, which took more time. When we went to do this again we processed and embedded marker tissues in the block for orientation, we used three long pieces of liver around the specimen to help line up the sections. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ftulenko06@jcu.edu Sent: Tuesday, February 27, 2007 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] serial sections Dear all, I have been trying to use serial sections (generated from paraplast-embedded chick embryos) to make three dimensional reconstructions of various skeletal structures. The problem I encounter is that the sections seem to move on the slides during drying (thus making smooth reconstructions impossible). Does anyone have advice on how to minimize section movement on slides? The slides I am using are Fisherbrand superfrost/Plus slides. Thank you for your times and suggestions. Sincerely, Frank _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From rjbuesa <@t> yahoo.com Tue Feb 27 14:40:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 27 14:40:16 2007 Subject: [Histonet] serial sections In-Reply-To: <20070227151503.ACL64347@mirapoint.jcu.edu> Message-ID: <589011.94737.qm@web61223.mail.yahoo.com> To avoid sections to move while drying there has to be eliminated the water underneath the section first. To accomplish this set the slides with the sections vertically (on the 1 inch end) for several minutes. After that take the slides and give them a gently shake (to eliminate any water near the bottom end), place them in the racks and let them dry overnight at room temperature. Next morning to the oven at 60?C for 30 minutes. It has worked always fine for me. Ren? J. ftulenko06@jcu.edu wrote: Dear all, I have been trying to use serial sections (generated from paraplast-embedded chick embryos) to make three dimensional reconstructions of various skeletal structures. The problem I encounter is that the sections seem to move on the slides during drying (thus making smooth reconstructions impossible). Does anyone have advice on how to minimize section movement on slides? The slides I am using are Fisherbrand superfrost/Plus slides. Thank you for your times and suggestions. Sincerely, Frank _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From pamvlies <@t> sbcglobal.net Tue Feb 27 17:02:10 2007 From: pamvlies <@t> sbcglobal.net (Pam V) Date: Tue Feb 27 17:02:24 2007 Subject: [Histonet] Re: Histonet Digest, Vol 39, Issue 44 Message-ID: <983214.69240.qm@web81108.mail.mud.yahoo.com> Are you Experienced is a Jimi Hendrix album...His first I believe...awesome stuff... Pam Vlies HT-ASCP Evanston Northwestern Hospital Evanston, IL pamvlies@sbcglobal.net ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 27, 2007 12:00:50 PM Subject: Histonet Digest, Vol 39, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Purple Haze (Fred Underwood) 2. Thermo Shandon Laser Slide Labeller (Laurie Elmgren) 3. PMS2 for IVD (Dana Settembre) 4. Histology Technicians Needed (M C) 5. please unsubscribe (Histology Field) 6. Anyone need some help... (M C) 7. Blue Feulgen Stain (Crystal Morris) 8. Dispensable knife (Marcia Funk) 9. RE: Purple Haze (Janice Mahoney) 10. RE: Purple Haze (Weems, Joyce) 11. RE: Dispensable knife (Cheryl Kerry) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Feb 2007 08:11:46 -0500 From: "Fred Underwood" Subject: RE: [Histonet] Purple Haze To: , , , , , Message-ID: Content-Type: text/plain; charset=US-ASCII How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 27 Feb 2007 09:52:17 -0500 From: "Laurie Elmgren" Subject: [Histonet] Thermo Shandon Laser Slide Labeller To: Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E321C4B7@MailPDC.sunriselab.com> Content-Type: text/plain; charset="iso-8859-1" Has anyone had any experience, comments about the Thermo Shandon Laser Slide Labeller? We would like to purchase it for use in a cyto and pathology lab setting. Very pricey, is it worth it? Laurie ------------------------------ Message: 3 Date: Tue, 27 Feb 2007 10:58:34 -0500 From: "Dana Settembre" Subject: [Histonet] PMS2 for IVD To: "louise renton" , Cc: Catherine Susan Delia , Meera Hameed Message-ID: Content-Type: text/plain; charset=US-ASCII Hello, I am looking for PMS2, colon cancer screening marker for in vitro diagnostic use on FFPE human tissue. Is there any? I've looking and looking. Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA ------------------------------ Message: 4 Date: Tue, 27 Feb 2007 08:23:46 -0800 From: "M C" Subject: [Histonet] Histology Technicians Needed To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed ***Open Positions for Laboratory Technicians available in New Histology Lab located in Southern California. Licensing not necessary. For more details, please reply to e-mail.*** -Marco Corona _________________________________________________________________ With tax season right around the corner, make sure to follow these few simple tips. http://articles.moneycentral.msn.com/Taxes/PreparationTips/PreparationTips.aspx?icid=HMFebtagline ------------------------------ Message: 5 Date: Tue, 27 Feb 2007 08:30:10 -0800 (PST) From: Histology Field Subject: [Histonet] please unsubscribe To: histonet@lists.utsouthwestern.edu Message-ID: <781988.39440.qm@web59201.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. ------------------------------ Message: 6 Date: Tue, 27 Feb 2007 08:42:41 -0800 From: "M C" Subject: [Histonet] Anyone need some help... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed If anyone needs some help finding these pieces of equipment: Linistat H&E Stainer E300 DRS-601 VIP2 Microm HM 355 Microtome I have them. Reply to my post for more details. Thanks, Marco _________________________________________________________________ With tax season right around the corner, make sure to follow these few simple tips. http://articles.moneycentral.msn.com/Taxes/PreparationTips/PreparationTips.aspx?icid=HMFebtagline ------------------------------ Message: 7 Date: Tue, 27 Feb 2007 11:59:54 -0500 From: "Crystal Morris" Subject: [Histonet] Blue Feulgen Stain To: Message-ID: <8F60E26ED772F54C8B1D39AAEC6ADA4901C34CF7@ex03.fmchealth.org> Content-Type: text/plain; charset="iso-8859-1" Is there anyone who is currently doing the blue feulgen stain and scoring it on the Chromavision Acis or an equivalent imaging system? If so can you tell me how this is going for your facility and in what capacity are you using it? Our pathologists want to use it for the bronchial biopsies that are going to be collected using an UV light endoscope. Is anyone doing this? Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." ------------------------------ Message: 8 Date: Tue, 27 Feb 2007 12:29:41 -0500 From: "Marcia Funk" Subject: [Histonet] Dispensable knife To: histonet@lists.utsouthwestern.edu Message-ID: <45E4162502000016003EB537@nodcmsngwia1.trinity-health.org> Content-Type: text/plain; charset=us-ascii Howdy, I would like to look at different microtome blades. We are using Accu-Edge Low Profile and they seem a bit pricy. I would like info on what types your lab is using . ------------------------------ Message: 9 Date: Tue, 27 Feb 2007 11:45:33 -0600 From: "Janice Mahoney" Subject: RE: [Histonet] Purple Haze To: , ,, , "Fred Underwood" , , Message-ID: <45E419DD0200003C00006841@gwia.alegent.org> Content-Type: text/plain; charset=US-ASCII Ah, How about the 60's. I can tell some of you younger people don't know the difference between Purple Haze and Purple Rain. Purple Haze is indeed Jimmy and I'm sure the cause of Purple Haze in those days was LSD not paraffin. >>> "Fred Underwood" 02/27/2007 7:11 AM >>> How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 27 Feb 2007 12:49:05 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Purple Haze To: "Janice Mahoney" , , , , , "Fred Underwood" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D356D@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" oh my goodness... I am one of those... Joe the Toe.. I apologize. I should know better but I am too young.....j -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Tuesday, February 27, 2007 12:46 PM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; Fred Underwood; jnocito@satx.rr.com; Weems, Joyce Subject: RE: [Histonet] Purple Haze Ah, How about the 60's. I can tell some of you younger people don't know the difference between Purple Haze and Purple Rain. Purple Haze is indeed Jimmy and I'm sure the cause of Purple Haze in those days was LSD not paraffin. >>> "Fred Underwood" 02/27/2007 7:11 AM >>> How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 11 Date: Tue, 27 Feb 2007 11:58:20 -0600 From: "Cheryl Kerry" Subject: RE: [Histonet] Dispensable knife To: "'Marcia Funk'" , Message-ID: <001201c75a98$df189ed0$6401a8c0@FSDESKTOP> Content-Type: text/plain; charset="us-ascii" While I don't have a list of other blade brands for you-- I do have a few things to add to your list when considering cost of blades in general. Have you calculated how many slides or blocks your techs produce per box of blades? AccuEdge blades do seem to be pricey but I've always gone through more of most of the other kinds...price per slide was uniformly better with low-profile AccuEdge and a thicker one like Sturkey for bone and hard tissue. Also consider the frustration factor. I don't care HOW much the blades cost when I'm trying to get 40 slides off a hair-thin prostate biopsy! In fairness, it takes a couple of days with a new blade to get used to the differences. Having both types around during a new blade trial reduces the fear factor and keeps people's minds a little more receptive to the changes. Some labs stock three or four different types--each tech picks what works for them and is responsible to keep the order clerk informed of when they need more. My two cents :-) Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, February 27, 2007 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dispensable knife Howdy, I would like to look at different microtome blades. We are using Accu-Edge Low Profile and they seem a bit pricy. I would like info on what types your lab is using . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 39, Issue 44 **************************************** From laurie.reilly <@t> jcu.edu.au Tue Feb 27 17:17:44 2007 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Tue Feb 27 17:17:53 2007 Subject: [Histonet] Omega recyclers Message-ID: <000701c75ac5$7c6fb020$de55db89@health.ad.jcu.edu.au> Histonetters, Has anyone had any experience using an "Omega" recycler. Regards, Laurie. Mr. Laurie Reilly School of Veterinary & Biomedical Sciences James Cook University Townsville Queensland 4811 Australia Phone 07 4781 4468 Fax 07 4779 1526 From Malcolm.McCallum <@t> tamut.edu Tue Feb 27 21:08:07 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Tue Feb 27 21:09:33 2007 Subject: [Histonet] help with tumor ID References: <7DBCCC1FBC77C94F99F920D0CA6400B61E404C@exchsrv01.barrynet.barry.edu> Message-ID: My email was down for the four days after I sent this, so I figured I'ld send again! I had some students doing skeletochronology on frog legs. I have a strange section that appears to be possible hyperplasia of the periosteum. It apears to be a proliferation of poorly-staining connective tissue (H-E stain). Not sure though. Anyone out there willing to take a look at a jpg and tell me what they think? VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html Spring Teaching Schedule & Office Hours: Monday: Genetics 1-2:40 pm Office Hours 4-6 pm Landscape Ecology 6-9:40 pm Tuesday Ichthyology 10-11:40 pm Office Hours/Student Research 1-2:30 pm Seminar 2:30-3:30 pm Wednesday Genetics 1-2:40 pm Office Hours/Student Research 3-5 pm Thursday Ichthyology 10-11:40 pm Office Hours/Student Research 1-4:30 pm ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Malcolm McCallum Sent: Wed 2/21/2007 7:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help with tumor ID Hi, I had some students doing skeletochronology on frog legs. I have a strange section that appears to be possible hyperplasia of the periosteum. It apears to be a proliferation of poorly-staining connective tissue (H-E stain). Not sure though. Anyone out there willing to take a look at a jpg and tell me what they think? VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html Spring Teaching Schedule & Office Hours: Monday: Genetics 1-2:40 pm Office Hours 4-6 pm Landscape Ecology 6-9:40 pm Tuesday Ichthyology 10-11:40 pm Office Hours/Student Research 1-2:30 pm Seminar 2:30-3:30 pm Wednesday Genetics 1-2:40 pm Office Hours/Student Research 3-5 pm Thursday Ichthyology 10-11:40 pm Office Hours/Student Research 1-4:30 pm ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Smith, Allen Sent: Wed 2/21/2007 7:18 PM To: MVaughan4@ucok.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peripheral nerve stain Kiernan's "physical developer method" for axons (J.A. Kiernan HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., 1999, pp. 371-373)is the most sensitive stain that I know of. It will beautifully demonstrate tiny nerve endings that the Holmes method misses and the Winkelmann method just barely stains. Expect it to take several tries until you get the timing of it just right for your tissue. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MVaughan4@ucok.edu Sent: Wednesday, February 21, 2007 6:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral nerve stain Histonetters, I would like to stain a skin section to view nerve fibers, endings or corpuscles. Are there any specific stains that will pick up these structures in paraffin embedded, formalin-fixed tissues? One stain I have seen listed is erythrosin B and methylene blue, but I haven't seen a protocol for this stain. Any others? Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Tue Feb 27 21:14:40 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Feb 27 21:14:46 2007 Subject: [Histonet] reference for the nail protocol Message-ID: We are using this Ammonium hydroxide protocol, but I don't have a clear reference... We've "been doing it forever".... Do you think I'll get dinged by CAP if my procedure manual indicates "Joe the Toe" as my reference? Bec Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 Message: 16 Date: Thu, 22 Feb 2007 17:03:08 -0600 From: "Joe Nocito" Subject: Re: [Histonet] PAS stain for nail fungus To: , Message-ID: <00c901c756d5$9e5609d0$649eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hey Doc, you have the South Texas Toenail lab guy right here. That's why I'm known as "Joe the Toe". We soak our nails in 20% ammonium hydroxide for at least an hour before we put the blocks on the machine to process. We've been getting nailed for a couple of years and perform a PAS for Fungus on each case that we do. Reimbursement must be good, we keep getting nailed. Joe the Toe or just JTT From vazquezr <@t> ohsu.edu Wed Feb 28 09:11:19 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Feb 28 09:11:55 2007 Subject: [Histonet] Cryocut 1800 accessories Message-ID: Greg, I got mine from Thermo and they were quite cheaper. The shaft was a little long, I had someone in facilities to cut them to the right length. It was worth the price difference. Robyn OHSU >>> "greg tesdall" 2/26/2007 12:50 PM >>> I'm looking to purchase 30mm specimen discs for a Leica Cryocut 1800 cryostat. Can anyone direct me to a vender. Thanks, Greg --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wickersham <@t> olympus-sis.com Wed Feb 28 09:25:32 2007 From: Jason.Wickersham <@t> olympus-sis.com (Jason Wickersham) Date: Wed Feb 28 09:26:14 2007 Subject: [Histonet] Purple Haze In-Reply-To: <200702271828.l1RIS616009669@mail.soft-imaging.de> References: <200702271828.l1RIS616009669@mail.soft-imaging.de> Message-ID: <78B53BA1C5A2D9449EDA30A98800EC947FC14B@ms-s-gws.soft-imaging.net> ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Wed Feb 28 09:34:39 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Feb 28 09:35:02 2007 Subject: [Histonet] Missouri Soc. For Histotechnology Symposium 2007 Message-ID: Missouri Society for Histotechnology 30th ANNUAL SPRING SYMPOSIUM Thursday, May 31st June 1st and 2nd, 2007 Lodge of the Ozarks 3431 W. Hwy 76 Branson, MO 65616 1-800-213-2584 Speakers Include: Stanley Weglarz, James B. McCormick, M.D., Lee Dickey, H. Skip Brown Joel Martinez, Pam Hesch, Jean-Philippe REY, Lisa Jackson, Sharad Mathur, M.D., Charlie Dorner, Jan Mahoney, Konnie Zeitner Thursday Evening Topic: Instrument Maintenance & Repair Friday Topics Include: Techniques for Management of Small Tissue Specimens Immunohistochemistry Math in the Laboratory Motivation and Team Development Basic Immunohistochemistry & More Saturday Topics Include: Birth of a Protein Fixation & Processing Moh's Micrographic Surgery. What is it? Algorithmic Methods for IHC (A practical approach to IHC Panels) Lab Safety (It's mostly a chemical thing or how not to experience the "big bang") How "Lean" can be Applied to the Histology Lab Introduction to Immunohistochemistry. (ie: where do I find it and what do I do with it?) Final Brochure to be mailed in mid March! For Information Contact: Sharon Walsh userwalsh(at)sbcglobal(dot)net Teri Johnson tjj(at)stowers-institute(dot)org Interested exhibitors not already contacted: JP Rey jpr(at)stowers-institute(dot)org From ree3 <@t> leicester.ac.uk Wed Feb 28 09:36:45 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Feb 28 09:36:56 2007 Subject: [Histonet] Purple Haze In-Reply-To: <78B53BA1C5A2D9449EDA30A98800EC947FC14B@ms-s-gws.soft-imaging.net> References: <200702271828.l1RIS616009669@mail.soft-imaging.de> <78B53BA1C5A2D9449EDA30A98800EC947FC14B@ms-s-gws.soft-imaging.net> Message-ID: HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kjohnson <@t> covx.com Wed Feb 28 10:37:47 2007 From: kjohnson <@t> covx.com (Kimberly Johnson) Date: Wed Feb 28 10:37:53 2007 Subject: [Histonet] question for all the mouse brain people out there! In-Reply-To: <26BE9ACC202D29479B0A144066A733E50223FE2F@phdex01.txhealth.org> Message-ID: Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim From TJJ <@t> Stowers-Institute.org Wed Feb 28 10:54:23 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Feb 28 10:54:48 2007 Subject: [Histonet] Effect of formamide solutions on proteins Message-ID: To all you chemist-types out there, What effect might 50% formamide solutions have on lightly formalin-fixed proteins? I know what affect is has on DNA, but what about other structural proteins in the cell? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From JWEEMS <@t> sjha.org Wed Feb 28 11:00:52 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Feb 28 11:01:30 2007 Subject: [Histonet] question for all the mouse brain people out there! Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D35B1@sjhaexc02.sjha.org> You must quit calling names if you want them to help!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kimberly Johnson Sent: Wednesday, February 28, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question for all the mouse brain people out there! Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From PMonfils <@t> Lifespan.org Wed Feb 28 11:07:35 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Feb 28 11:07:44 2007 Subject: [Histonet] question for all the mouse brain people out there! In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C4C@LSRIEXCH1.lsmaster.lifespan.org> Leaving them for 4 days won't make any difference compared with leaving them for 2 days. In 2 days they are completely infiltrated. In 4 days they will still be completely infiltrated. (I assume you are keeping them in the refrigerator!) > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kimberly Johnson > Sent: Wednesday, February 28, 2007 8:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] question for all the mouse brain people out there! > > Hello Histonet! > I have a quick question for you mouse brain people out there who have > been so very helpful to me in the past. I usually keep my brains in 30% > sucrose for 2 days, then freeze them for sectioning. Would I ruin them > if I kept them in the sucrose for 4 days? I am trying to get out of > coming in on the weekend, so all you input would be greatly appreciated! > > Thanks so much! > > Kim > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Wed Feb 28 11:10:09 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 28 11:10:17 2007 Subject: [Histonet] question for all the mouse brain people out there! In-Reply-To: Message-ID: <167250.20591.qm@web61219.mail.yahoo.com> Kimberly: You know that the circumstances vary from lab to lab. In your case I would do as follows: instead on relying on somebody elses experience on the subject, I woul keep one mouse brain in sucrose for 4 days (under my specific set of circumstances and environment characteristics) and determine what happens. Just a thought. Ren? J. Kimberly Johnson wrote: Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From TJJ <@t> Stowers-Institute.org Mon Feb 26 14:23:09 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Feb 28 11:35:26 2007 Subject: [Histonet] Missouri Soc. For Histotechnology Symposium 2007 Message-ID: Missouri Society for Histotechnology 30th ANNUAL SPRING SYMPOSIUM Thursday, May 31st June 1st and 2nd, 2007 Lodge of the Ozarks 3431 W. Hwy 76 Branson, MO 65616 1-800-213-2584 Speakers Include: Stanley Weglarz, James B. McCormick, M.D., Lee Dickey, H. Skip Brown Joel Martinez, Pam Hesch, Jean-Philippe REY, Lisa Jackson, Sharad Mathur, M.D., Charlie Dorner, Jan Mahoney, Konnie Zeitner Thursday Evening Topic: Instrument Maintenance & Repair Friday Topics Include: Techniques for Management of Small Tissue Specimens Immunohistochemistry Math in the Laboratory Motivation and Team Development Basic Immunohistochemistry & More Saturday Topics Include: Birth of a Protein Fixation & Processing Moh's Micrographic Surgery. What is it? Algorithmic Methods for IHC (A practical approach to IHC Panels) Lab Safety (It's mostly a chemical thing or how not to experience the "big bang") How "Lean" can be Applied to the Histology Lab Introduction to Immunohistochemistry. (ie: where do I find it and what do I do with it?) Final Brochure to be mailed in mid March! For Information Contact: Sharon Walsh userwalsh(at)sbcglobal(dot)net Teri Johnson tjj(at)stowers-institute(dot)org Interested exhibitors not already contacted: JP Rey jpr(at)stowers-institute(dot)org From cwscouten <@t> myneurolab.com Wed Feb 28 11:56:12 2007 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Wed Feb 28 11:55:44 2007 Subject: [Histonet] question for all the mouse brain people out there! References: <167250.20591.qm@web61219.mail.yahoo.com> Message-ID: <5784D843593D874C93E9BADCB87342AB027D9C3A@tpiserver03.Coretech-holdings.com> However, if you need some information right now, if they are fixed in formaldehyde before going in the sucrose, they will keep for months, but to some degree depending on what you want to do with them. Antigenicity might change, Nissl staining won't. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 28, 2007 11:10 AM To: Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question for all the mouse brain people out there! Kimberly: You know that the circumstances vary from lab to lab. In your case I would do as follows: instead on relying on somebody elses experience on the subject, I woul keep one mouse brain in sucrose for 4 days (under my specific set of circumstances and environment characteristics) and determine what happens. Just a thought. Ren? J. Kimberly Johnson wrote: Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jengirl1014 <@t> yahoo.com Wed Feb 28 12:12:52 2007 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Wed Feb 28 12:13:03 2007 Subject: [Histonet] shipment Message-ID: <950521.7322.qm@web62401.mail.re1.yahoo.com> I need to ship some mouse kidney. I know the protocol that we have requires that it sits in the 4% PFA for approximately 5 - 6 hours. They want to process it themselves. How would you recommend I do this? Thanks, Jen --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From POWELL_SA <@t> Mercer.edu Wed Feb 28 12:29:01 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Wed Feb 28 12:29:40 2007 Subject: [Histonet] Deadline tomorrow Message-ID: <01MDO179HCEK8WYHQP@Macon2.Mercer.edu> Hi Guys, Another friendly reminder to make your reservations for GSH 's meeting April 13-15 at Callaway Gardens GA. The programs and vendor registration forms can be downloaded from our website at www.histosearch.com/gsh. The deadline for the hotel reservations is tomorrow, March 1st, in order to take advantage of the great rate for our meeting - $119 for single, double, triple or quad, and this is their peak season and after tomorrow the rates go up. Vendors can download the exhibit registration form and sponsorship form from the symposium page on our site. Callaway Gardens GA is a beautiful place to visit this time of year. The gardens and The Day Butterfly Center are outstanding. The other attractions are the golfing, fly fishing, bass fishing, educational tours, tennis, swimming, boating, shopping, and the beautiful Sibley Horticulture Center, The Callaway Discovery Center, The Pioneer Log Cabin, the Gardens' bike and walking trails, Mr. Cason's Vegetable Garden, and all of the area's scenery and flowers. Bring your family to enjoy the Gardens and the beautiful weather we are having here in Georgia. In the 70s today. Shirley Powell GSH Secretary/Meeting Registrar From MDiCarlo <@t> KaleidaHealth.Org Wed Feb 28 13:06:08 2007 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Wed Feb 28 13:06:22 2007 Subject: [Histonet] fabric softener Message-ID: <9B4A77DF11463E4FB723D484214AE9BC02897A89@KALEXMB02.KaleidaHealth.org> Histonetters, After I'm done decaling a large human bone, rinse in running tap H2O for two hours, then soak in 1% fabric softener for an hour, how long should I rinse the bone after the fabric softener before I start processing in 70%? I've never done this but lately I have had great difficulty sectioning large bones from being so hard that it skips over part of the bone. Thanks for your help. Peggy DiCarlo HT (ASCP) Orthopaedics Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From sbreeden <@t> nmda.nmsu.edu Wed Feb 28 13:15:54 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Feb 28 13:16:03 2007 Subject: [Histonet] GRAM Stain Timing Question Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F435F@nmdamailsvr.nmda.ad.nmsu.edu> I seem to do a lot of GRAM stains and although I've modified some of the times to suit my pathologists' tastes, I have never been able to pin down the first differentiation time in 100% alcohol. Today, I'm using 10 quick dips although it's only because the wind is blowing and the moon is on the rise. Can someone give me a more specific guideline for differentiating after the Gram's Iodine and before the Safranin? I'd like to leave a more definite legacy when I retire than "differentiate in 100% alcohol or acetone"... I'm thankin' you in advance. And I think we all know who the Purple Haze Generation are on THIS media, don't we??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From AGrobe2555 <@t> aol.com Wed Feb 28 13:25:09 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Feb 28 13:25:33 2007 Subject: [Histonet] Shipment Message-ID: I would call the people who need the tissue and ask them. Fill them in on your protocol and ask them how they prefer their tissue.....


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AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From lblazek <@t> digestivespecialists.com Wed Feb 28 13:47:58 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Feb 28 13:44:32 2007 Subject: [Histonet] GRAM Stain Timing Question References: <4D14F0FC9316DD41972D5F03C070908B8F435F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6CBA6DC98A079D408C87250591D9DFB802360DFC@bruexchange.digestivespecialists.com> Can't your legacy be "differentiate until it looks right"? If I recall right (and that is a question in it's self) when the color stops running off the slide the differentiation is complete. To go any further you will decolorize the gram-positive thingy bobs. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 28, 2007 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GRAM Stain Timing Question I seem to do a lot of GRAM stains and although I've modified some of the times to suit my pathologists' tastes, I have never been able to pin down the first differentiation time in 100% alcohol. Today, I'm using 10 quick dips although it's only because the wind is blowing and the moon is on the rise. Can someone give me a more specific guideline for differentiating after the Gram's Iodine and before the Safranin? I'd like to leave a more definite legacy when I retire than "differentiate in 100% alcohol or acetone"... I'm thankin' you in advance. And I think we all know who the Purple Haze Generation are on THIS media, don't we??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.owen <@t> fda.hhs.gov Wed Feb 28 13:34:11 2007 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Wed Feb 28 13:46:40 2007 Subject: [Histonet] GRAM Stain Timing Question In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F435F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <15B0433665D1724BAE49F6E4476AD81C4D107A@FMD3VS012.fda.gov> Dear Sara, In my laboratory personnel when using Coplin jars Gram staining is usually performed as below. I have good results most of the time following this procedure. Crystal Violet: 60 seconds Tap Water Rinse Iodine: 60 seconds Tap Water Rinse 50% w/v Acetone-95% Ethanol: 40 seconds Tap Water Rinse Safranin: 60 seconds Tap Water Rinse and Air Dry My laboratory usually has Remel or Difco/BD Gram stain kits. The decolorizer runs out quickly therefore I often make my own that is equivalent to ones found in these kits. Sincerely, Michael Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From rjbuesa <@t> yahoo.com Wed Feb 28 14:40:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 28 14:40:24 2007 Subject: [Histonet] GRAM Stain Timing Question In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F435F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <870079.89942.qm@web61219.mail.yahoo.com> Sara: This is how I used to do it (to everybody's acceptance): 1-dewax 2-slides horizontal in a rack flooded with crystal violet and 5 drops of bicarbonate-phenol x 5 min 3-rinse one at a time in running water and return to the staining rack to be flooded with Grams's iodine x 5 min 4- one at a time rinse in tap water and BLOT with paper towels 5- one at a time immerse in acetone UNTIL NO MORE BLUE comes out of the slide (usually 1-2 dips in acetone) 6- quick rinse in tap water and place in Coplin jar 7- add 40 mL of distilled water in the Colplin jar + 3 mL of alcoholic basic fuchsin x 5 min 8-after that blot (do not rinse) with paper towels one at a time 9- prepare 5 Coplin jars (3 with acetone + 2 with xylene) 10-in the first one add 40 mL of acetone + 7 mL of stock picro-acetone sol. 11-one at a time run the slides in the 5 Coplin jars. In the first immerse the slides until no more red comes out (usually 1-2 dips). 12-after that move quickly the slides through the other 4 Coplin jars 13-leave in xylene until coverslip. To your specific question: see #5 (until no more blue comes out of the section). Hope this will help you! Ren? J. "Breeden, Sara" wrote: I seem to do a lot of GRAM stains and although I've modified some of the times to suit my pathologists' tastes, I have never been able to pin down the first differentiation time in 100% alcohol. Today, I'm using 10 quick dips although it's only because the wind is blowing and the moon is on the rise. Can someone give me a more specific guideline for differentiating after the Gram's Iodine and before the Safranin? I'd like to leave a more definite legacy when I retire than "differentiate in 100% alcohol or acetone"... I'm thankin' you in advance. And I think we all know who the Purple Haze Generation are on THIS media, don't we??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From pruegg <@t> ihctech.net Wed Feb 28 14:44:30 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 28 14:44:46 2007 Subject: [Histonet] question for all the mouse brain people out there! In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D35B1@sjhaexc02.sjha.org> Message-ID: <001d01c75b79$3f6013a0$6501a8c0@Patsy> That made me laugh Joyce! Patsy Are you a mouse brained person??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 28, 2007 10:01 AM To: Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! You must quit calling names if you want them to help!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kimberly Johnson Sent: Wednesday, February 28, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question for all the mouse brain people out there! Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pruegg <@t> ihctech.net Wed Feb 28 14:48:46 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 28 14:48:54 2007 Subject: [Histonet] Effect of formamide solutions on proteins In-Reply-To: Message-ID: <001e01c75b79$d83dfce0$6501a8c0@Patsy> Teri, I am not much of a chemist-type but if the proteins are not protected by cross-linking with formaldehyde then using any solvent especially one a potent as formamide can really reek havoc, especially on surface proteins, sounds like a bad idea to me, without 24hr in formalin. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Wednesday, February 28, 2007 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Effect of formamide solutions on proteins To all you chemist-types out there, What effect might 50% formamide solutions have on lightly formalin-fixed proteins? I know what affect is has on DNA, but what about other structural proteins in the cell? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Feb 28 14:49:59 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Feb 28 14:50:31 2007 Subject: [Histonet] question for all the mouse brain people out there! Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D35BE@sjhaexc02.sjha.org> No, just a bird brain...:>) getting more like a turkey every day. -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Wednesday, February 28, 2007 3:45 PM To: Weems, Joyce; 'Kimberly Johnson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! That made me laugh Joyce! Patsy Are you a mouse brained person??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 28, 2007 10:01 AM To: Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! You must quit calling names if you want them to help!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kimberly Johnson Sent: Wednesday, February 28, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question for all the mouse brain people out there! Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Eric.C.Kellar <@t> questdiagnostics.com Wed Feb 28 14:59:17 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Wed Feb 28 14:59:43 2007 Subject: [Histonet] GRAM Stain Timing Question Message-ID: <6843061CE6B98E4B96590D4F299618F801583C05@qdcws0117.us.qdx.com> According to Bartholomew and Mittwer (1950,1951), the mechanism of stainability can be explained by differences in the permeability of the organism membrane. The bacteria stain by linkage between acid groups of the bacteria and alkaline groups of dye. Iodine forms a complex with the dye and this complex is dissociated by alcohol. If alcohol passes easily through the membrane, decolorization is rapid and the reaction is Gram negative. If the membrane is hardly or not at all penetrated by alcohol, the reaction is Gram positive. The condition of the membrane of the organism is the determining factor. Horobin and Bancroft say that, if specimen slides are taken into differentiator when still wet, then de-colorization can be many times faster, leading to dye loss and thus an apparent lack of Gram positive organisms. Standardize your procedure by always introducing specimens into differentiator either wet or blotted dry and always run a known Gram positive/negative control. The histologist Gram who introduced the method in 1884 states that if you differentiate in alcohol, "Differentiate until no more stain comes away". If using acetone, "Flood slides for not more than 2-5 seconds". Still works for me! Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 28, 2007 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GRAM Stain Timing Question I seem to do a lot of GRAM stains and although I've modified some of the times to suit my pathologists' tastes, I have never been able to pin down the first differentiation time in 100% alcohol. Today, I'm using 10 quick dips although it's only because the wind is blowing and the moon is on the rise. Can someone give me a more specific guideline for differentiating after the Gram's Iodine and before the Safranin? I'd like to leave a more definite legacy when I retire than "differentiate in 100% alcohol or acetone"... I'm thankin' you in advance. And I think we all know who the Purple Haze Generation are on THIS media, don't we??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From LSebree <@t> uwhealth.org Wed Feb 28 15:08:36 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Feb 28 15:08:46 2007 Subject: [Histonet] Secondary antibody for Ventana detection kits Message-ID: Hello, I have the need to optimize a goat anti-human antibody on the Ventana XT immunostainer. In order to incorporate an anti-goat secondary I am using their "open secondary" protocol with an iView DAB detection kit. I am wondering whose antibody people are using in these circumstances. Ventana says that people have used Jackson ImmunoResearch's biotinylated R x G F(ab')2 (cat. #: 305-066-003) for this purpose with success. I would like to actually talk to anyone who has done this as I have concerns about their storage requirements and putting this antibody in a dispenser. Thank you in advance for your help, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From rgrow <@t> bmnet.com Wed Feb 28 15:37:37 2007 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Wed Feb 28 15:37:48 2007 Subject: [Histonet] Re: LIS porgrams In-Reply-To: Message-ID: We use WindoPath by Psyche Systems. It opens to an office display. You click on the microscope to ascession, a file drawer to review current cases, a file cabinet for archives, a photo to find physicians contact info, and a bookcase to /build list sources/charge codes! You can add/delete blocks, special stains, IHC, etc. The charge code is attached to the source, so if the pathologist decides the alcian blue wasn't necessary, when the stain is removed, so is the charge code. It can be customized to fit your institution needs, and if you have any problems, it's possible the owner/developer of the system may be the one to solve your problem! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn McDonald Sent: Thursday, February 22, 2007 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS programs What is your favorite Pathology program? What do you like about it? I am familiar with Tamtron (Powerpath). A friend has asked me so I decided to post it on the Histonet. No vendors please. Thanks From SDrew <@t> uwhealth.org Wed Feb 28 15:53:19 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Feb 28 15:53:29 2007 Subject: [Histonet] anti-SSX staining Message-ID: Is anyone running anti-SSX on the Ventana immunostainer? I am trying to get Santa Cruz SSX(FL-188) to work-I tried it on breast carcinoma slides and a couple of rhabdomyosarcoma slides with no luck. Thank you for anything you've got to say... Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From SDrew <@t> uwhealth.org Wed Feb 28 15:55:35 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Feb 28 15:55:43 2007 Subject: [Histonet] major oops-I meant MDM2 Message-ID: I'm working on too many antibodies at once! I actually am asking about LabVision/NeoMarker's MDM2 antibody! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From pruegg <@t> ihctech.net Wed Feb 28 16:14:57 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 28 16:15:10 2007 Subject: [Histonet] Secondary antibody for Ventana detection kits In-Reply-To: Message-ID: <005801c75b85$e233ebe0$6501a8c0@Patsy> Linda, Can't help you with Ventana application but I did want to mention that BioCare now sells an anti-goat labeled polymer hrp detection system that works well. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Wednesday, February 28, 2007 2:09 PM To: Histonet Subject: [Histonet] Secondary antibody for Ventana detection kits Hello, I have the need to optimize a goat anti-human antibody on the Ventana XT immunostainer. In order to incorporate an anti-goat secondary I am using their "open secondary" protocol with an iView DAB detection kit. I am wondering whose antibody people are using in these circumstances. Ventana says that people have used Jackson ImmunoResearch's biotinylated R x G F(ab')2 (cat. #: 305-066-003) for this purpose with success. I would like to actually talk to anyone who has done this as I have concerns about their storage requirements and putting this antibody in a dispenser. Thank you in advance for your help, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Wed Feb 28 17:19:10 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Feb 28 17:19:49 2007 Subject: [Histonet] Secondary antibody for Ventana detection kits Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F99BA@NVCIEXCH02.NVCI.org> I've done this before. I just filled a prep kit with a the biotin-conjugated secondary against goat (I used one from miltinyi and I think the dilution I used was 1:200), then I photocopied the bar code from Ventana's biotinylated secondary, put it on the prep kit, filled it up, and replaced Ventana's secondary. I ran it by alone since that secondary wouldn't pick up a signal from my normal mouse and rabbit antibodies. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Wednesday, February 28, 2007 1:09 PM To: Histonet Subject: [Histonet] Secondary antibody for Ventana detection kits Hello, I have the need to optimize a goat anti-human antibody on the Ventana XT immunostainer. In order to incorporate an anti-goat secondary I am using their "open secondary" protocol with an iView DAB detection kit. I am wondering whose antibody people are using in these circumstances. Ventana says that people have used Jackson ImmunoResearch's biotinylated R x G F(ab')2 (cat. #: 305-066-003) for this purpose with success. I would like to actually talk to anyone who has done this as I have concerns about their storage requirements and putting this antibody in a dispenser. Thank you in advance for your help, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From ladylaynah <@t> yahoo.com Wed Feb 28 21:56:26 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Wed Feb 28 21:56:39 2007 Subject: [Histonet] MART-1 staining In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A11FFF7@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <639935.14218.qm@web37003.mail.mud.yahoo.com> Claire, I am doing the same thing in our Mohs lab, except that we are using MiTF (microphthalmia transcription factor from LabVision - NeoMarkers) antibody insteadof MART-1 because my doc's lit search showed that MART-1 tends to stain more promiscuously leading to ambiguity in the interpretation. Since you are using unfixed tissue, epitope retrieval is not needed. If you did an epitope retrieval step, that is one possibility to explain the staining problems. Epitope retrieval is used on fixed tissues. Did you optimize the antibody dilution? Did you block for catalase and protein? These may be some other causes of your problems. I was told to use HRP rather than AP on this assay, I did not get any good explanation why . Skin should be fine with AP, but maybe it has something to do with the antibody itself. Also, you didn't mention your sections. Everything I have read says that the frozen sections need to be nearly perfect and cut between 2 -4 microns, the thinner the better. If you want my protocol, email me and i will be happy to share. Connie McManus, HT University of Utah School of Medicine Dermatology --- Ingles Claire wrote: > Greetings all: > I have recently been the lucky one to be given the > task of trying to make MART-1 staining in our lab a > reality. (or try anyway) I have all the protocols > down for the most part. The only question I have is > if I have to do any kind of enzyme digestion to open > up the epitopes, etc and if so what kind. I am > working on frozen skin sections. They are frozen in > the cryostat and cut as soon as the tissue is > brought into the lab. We are a Mohs lab, so the > tissue hasn't been off the patient more than 10 > minutes when we get it. I am currently trying out > the Biocare prediluted MART-1, with the AP kit using > Vulcan Fast Red (permanent) as a chromogen. I pH'd > the buffer too before using and it was within > acceptable range. So far there has been no specific > staining, and only a little of what I would call > background or non-specific staining along the basal > layer where most of the melanocytes are located. The > staining stains the entire basal layer, not > individual areas where the known Melanoma areas are > located. I have also tried the Innovex HMB-45 > antibody with AP and fast red chromogen (aqueous) > with even less of a reaction. Those sections are > completely clean of any staining. Please help. I > would also appreciate any other suggestions you may > have. > > Thanks a bunch, > > Claire Ingles > Mohs Clinic > UW Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news From tahseen <@t> brain.net.pk Wed Feb 28 22:07:22 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Wed Feb 28 22:13:10 2007 Subject: [Histonet] block/slides storage References: <935308.36729.qm@web38011.mail.mud.yahoo.com> Message-ID: <008201c75bb7$1ed50c10$711480cb@piii> We are useing BioOptica storage system. Tahseen ----- Original Message ----- From: "Amy Lee" To: "histonet" Sent: Monday, February 26, 2007 11:47 PM Subject: [Histonet] block/slides storage > Hello histonetters, > > I am looking for a block/slides storage system. I work in industry. Could > anybody recommend a vender? > > Thanks, > Amy > > > --------------------------------- > Expecting? Get great news right away with email Auto-Check. > Try the Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Wed Feb 28 22:17:55 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Wed Feb 28 22:23:40 2007 Subject: [Histonet] External diameter of large vein Message-ID: <00a201c75bb8$971f00d0$711480cb@piii> Dear All, How can the external diameter of large vein like renal vein be measured on slide under light microscope? As the vein is large and measures several millimeters the micrometer scale cannot be used. we also do not want to measure grossly using a venire calipre. Our aim is to measure the vertical, oblique and transverse external diameter on the slide through microscope. Please also give the reference of the method. Thanks Dr Robina Shaheen Abbottabad, Pakistan From rchiovetti <@t> yahoo.com Wed Feb 28 23:57:57 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Wed Feb 28 23:58:03 2007 Subject: [Histonet] External diameter of large vein Message-ID: <18091.96451.qm@web58915.mail.re1.yahoo.com> Dr. Tahseen, Are you able to make digital pictures of the large veins at low magnification on the microscope? I would make a suggestion that you investigate using this approach. Even if you have to take several pictures to cover the entire field, the pictures can be combined or "stitched" together for measurement. Taking pictures in digital format, or taking pictures with film and then scanning them (to convert them to a digital format) would give you lots of possibilities to then use a computer to make the measurements. You would require measuring software to do this, and the software would have to be calibrated so it would give you the proper measurements and units. There are several good packages that would do this. A good place to start is the link below. The Software is called Image J. It is free, at least in the United States. It was developed by our National Institutes of Health. I don't know whether the link will allow you to download from a foreign country, but it's worth looking into. You can get details at: http://rsbweb.nih.gov/ij/features.html Best regards, Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Tahseen To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 28, 2007 9:17:55 PM Subject: [Histonet] External diameter of large vein Dear All, How can the external diameter of large vein like renal vein be measured on slide under light microscope? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 From Melanie.Black <@t> uct.ac.za Tue Feb 27 04:11:24 2007 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Fri Mar 2 04:05:32 2007 Subject: [Histonet] UNSUBSCRIBE!!!!! Message-ID: PLEASE UNSUBSCRIBE ME!!! Melanie Black Melanie.Black@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6589 021-448 5935 (fax) Mobile 0824693352 From Melanie.Black <@t> uct.ac.za Tue Feb 27 04:11:24 2007 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Fri Mar 2 04:05:37 2007 Subject: [Histonet] UNSUBSCRIBE!!!!! Message-ID: PLEASE UNSUBSCRIBE ME!!! Melanie Black Melanie.Black@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6589 021-448 5935 (fax) Mobile 0824693352