From yvan_lindekens <@t> yahoo.com Sat Dec 1 05:41:42 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Sat Dec 1 05:41:46 2007 Subject: [Histonet] Double skeletal stain in Zebrafish In-Reply-To: Message-ID: <707575.8313.qm@web30901.mail.mud.yahoo.com> http://www.bio.net/bionet/mm/zbrafish/1994-October/002903.html --- "Dickey, Coral" wrote: > > Hello, > > I am looking for a protocol for Alizarian red/Alcian > blue double skeletal stain on whole zebrafish. I > have a protocol for chick and mice embryos, but > wanted something more specific to the species I am > working with. > > Thanks, > > Coral Dickey > Histology Specialist I > > Stowers Institute for Medical Research > 1000 E. 50th Street > Kansas City, Missouri 64110 > Phone: 816-926-4305 > e-mail: cad@stowers-institute.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. http://mobile.yahoo.com/sports;_ylt=At9_qDKvtAbMuh1G1SQtBI7ntAcJ From rjbuesa <@t> yahoo.com Sat Dec 1 07:13:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 1 07:13:27 2007 Subject: [Histonet] Slide quality In-Reply-To: <648380.1758.qm@web57412.mail.re1.yahoo.com> Message-ID: <938416.498.qm@web61215.mail.yahoo.com> And that is the way it should be: every time a poor quality slide is produced, it should be documented but more importantly, it should be the "trigger" to re-train people. We all should try to get perfect slides. Ren? J. Sandy Smith wrote: When should the pathologist document wrinkles/folds? Only when it interferes with the diagnosis? Or any time there is a wrinkle or fold? Also how many histo techs out there get 100% wrinkle free sections? I have had discussions with my pathologists about these issues and would like others input. I believe any time there is a wrinkle or fold it should be documented. ____________________________________________________________________________________ Get easy, one-click access to your favorites. Make Yahoo! your homepage. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From cmiller <@t> physlab.com Sat Dec 1 08:23:49 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Sat Dec 1 08:23:47 2007 Subject: [Histonet] Slide quality In-Reply-To: <938416.498.qm@web61215.mail.yahoo.com> References: <648380.1758.qm@web57412.mail.re1.yahoo.com> <938416.498.qm@web61215.mail.yahoo.com> Message-ID: <008101c83425$ca65c280$3402a8c0@plab.local> How many wrinkles are we talking about??? I strive for perfect sections. I teach my students and preach to my techs the same. But to be realistic you could lose tissue in search of that perfect section, especially when your equipment is older and no funds to replace them. Some of us soak before we cut, others use ammonia. If perfect sections with no wrinkles are so easy to obtain just by technique alone, then why was our practicum so difficult?? I hear techs (older techs) talk about the hundreds of slides they cut before the "perfect one" I feel some expectations here are not realistic. Our pathologists are usually complimentary of our sections. Also, fixation is a huge factor and we don?t always have control of how the tissue was handled before we get it. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 01, 2007 7:13 AM To: Sandy Smith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide quality And that is the way it should be: every time a poor quality slide is produced, it should be documented but more importantly, it should be the "trigger" to re-train people. We all should try to get perfect slides. Ren? J. Sandy Smith wrote: When should the pathologist document wrinkles/folds? Only when it interferes with the diagnosis? Or any time there is a wrinkle or fold? Also how many histo techs out there get 100% wrinkle free sections? I have had discussions with my pathologists about these issues and would like others input. I believe any time there is a wrinkle or fold it should be documented. ____________________________________________________________________________ ________ Get easy, one-click access to your favorites. Make Yahoo! your homepage. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From amosbrooks <@t> gmail.com Sat Dec 1 21:35:20 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Dec 1 21:35:29 2007 Subject: [Histonet] Re: Histonet Digest, Vol 49, Issue 1 In-Reply-To: <47516e80.1186460a.5f86.620fSMTPIN_ADDED@mx.google.com> References: <47516e80.1186460a.5f86.620fSMTPIN_ADDED@mx.google.com> Message-ID: <582736990712011935u52e14b4dhc02ea098c95ed78@mail.gmail.com> Sandy, I think that we should all strive for perfect sections. Quality is important, but it is also important to factor in other issues. As has been previously mentioned the tissue loss can be an issue. It is also important to recognize the reason for imperfect sections. Unless you happen to live in the UK where everyone seems to have the time to manually process their tissue based on tissue type (wink, wink), odds are tissues are processed together and only slightly separated between biopsy and everything else. Good luck getting a perfectly sectioned spleen, breast, artery, muscle, and bone when they have all been processed together. Lets now toss in the docs submitting poorly trimmed tissue and not letting them fix. Obviously, the blame often (OK let's say it *usually*) rests on an impatent tech that just wants to get the pile of blocks cut. We need to be mindful of the results of haste. Try for perfect, of course. Settle for crap, never! We can only do our best and be open about learning from each other. If there's a problem fix it. I want people to point out the flaws in my sectioning so I know if I need to fix something. I also check the slides regularly before sending them out of my lab for this very reason. So record keeping can help there. We need to learn from our mistakes. But, you'll be really busy if you try to document every wrinkle, and unless you are very careful about how the issues are addressed (assuming every imperfection is noted), it will make for some very aprehensive techs. Balance is everything, Amos Brooks > > Message: 17 > Date: Fri, 30 Nov 2007 15:15:20 -0800 (PST) > From: Sandy Smith > Subject: RE: [Histonet] Slide quality > To: histonet@lists.utsouthwestern.edu > Message-ID: <648380.1758.qm@web57412.mail.re1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > When should the pathologist document wrinkles/folds? Only when it > interferes with the diagnosis? Or any time there is a wrinkle or fold? > Also how many histo techs out there get 100% wrinkle free sections? I have > had discussions with my pathologists about these issues and would like > others input. I believe any time there is a wrinkle or fold it should be > documented. From kemlo <@t> f2s.com Sun Dec 2 04:13:45 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sun Dec 2 04:14:28 2007 Subject: [Histonet] Re: Histonet Digest, Vol 49, Issue 1 In-Reply-To: <582736990712011935u52e14b4dhc02ea098c95ed78@mail.gmail.com> References: <47516e80.1186460a.5f86.620fSMTPIN_ADDED@mx.google.com> <582736990712011935u52e14b4dhc02ea098c95ed78@mail.gmail.com> Message-ID: <8E80221F387149D88F0350AEA70A7799@KemloPC> Unless you happen to live in the UK where everyone seems to have the time to manually process their tissue based on tissue type (wink, wink), odds are tissues are processed together and only slightly separated between biopsy and everything else. Good luck getting a perfectly sectioned spleen, breast, artery, muscle, and bone when they have all been processed together. Lets now toss in the docs submitting poorly trimmed tissue and not letting them fix. Amos" Oh I see fighting talk!! But as I note not only is it the processing that is suboptimal in the US but the fixation and care with sectioning too. I rest my case. Kemlo From sccrshlly <@t> yahoo.com Sun Dec 2 13:09:27 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Sun Dec 2 13:09:34 2007 Subject: [Histonet] Re: Orientation of Endoscopic Biopsies Message-ID: <694061.98105.qm@web90314.mail.mud.yahoo.com> Just a note on endoscopic specimens...I work in a lab that processes these specimens exclusively and we have found that using cassettes that do not require the use of sponges or filter paper or biopsy bags seem to allow the tissue to maintain it's natural shape. When a biopsy is taken from the GE tract, the tissue tends to curl into a "C" shape due to the tissue contracting when the biopsy is taken. This "C" shape is what helps us orient these biopsies so well. If you ensure the the "C" is put on edge, then the orientation will be correct. You can also face all the "C"'s in the same direction to ensure the epitheliums all line up (I know--just a little anal, but it all depends on what your pathologist wants :) )The other method we use is a piece of black paper at the embedding station. The tech who uses this actually places the biopsies on the paper. This allows her (she needs reading glasses) to be able to see the orientation of the tissue more clearly. Good luck Shelly From: "Walzer Susan" Subject: RE: [Histonet] ORIENTATION OF ENDOSCOPIC BIOPSIES CC: Date: Fri, 30 Nov 2007 03:40:45 -0500 To: I was under the impression that most biopsies done with this procedure were like skin shave bx's, that is that they are disc shaped usually and need to be embedded on end in order to see all layers of mucosa. Often they come in all shapes and we do the best we can. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, November 29, 2007 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ORIENTATION OF ENDOSCOPIC BIOPSIES Hi Does anyone have any suggetions (other than eosin in the processor) to assist in have the endoscopic biopsies orientated on the proper plane when embedded. I feel we have too high of a percentage that are not getting embedded properly. diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From ohenry <@t> dfw.net Sun Dec 2 13:17:30 2007 From: ohenry <@t> dfw.net (Susan Owens) Date: Sun Dec 2 13:15:38 2007 Subject: [Histonet] RE: Slide Quality Message-ID: <000701c83517$fc878940$1bf332d8@your4f1261a8e5> There should be a QC chart sent to the Pathologist daily with someone selecting a random H & E case..The Pathologist can check off such things as stain quality, wrinkles/folds etc....Poor sections should be addressed whether it interferes with the diagnosis or not..... There can be a problem within a certain section that makes it's early impossible for a wrinkle/fold fee selection...BUT, that would not be a daily issue...I would say any good Histo tech can and should have at least 99.% wrinkle/fold fee sections. If wrinkles/folds are routine, then that's a big problem that needs to be addressed and addressed now. >>> others input. I believe any time there is a wrinkle or fold it should >>> be >>> documented. I agree Sandy, BUT don't start a "witch hunt"...If you fine the poor quality sections coming from the same person(s) then a sit down talk with a walk down memory lane( as in back to basics) would be in order....Not a burning on the cross(at least not yet)... If wrinkles/folds are a big daily issue, then yes document, investigate and correct the problems..But if your talking about one wrinkle on one slide out of many slides.....then you can document, but don't go to war over it..... Susan > Message: 17 > Date: Fri, 30 Nov 2007 15:15:20 -0800 (PST) > From: Sandy Smith > Subject: RE: [Histonet] Slide quality > To: histonet@lists.utsouthwestern.edu > Message-ID: <648380.1758.qm@web57412.mail.re1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > When should the pathologist document wrinkles/folds? Only when it > interferes with the diagnosis? Or any time there is a wrinkle or fold? > Also how many histo techs out there get 100% wrinkle free sections? I > have > had discussions with my pathologists about these issues and would like > others input. I believe any time there is a wrinkle or fold it should be > documented. From rjbuesa <@t> yahoo.com Sun Dec 2 13:25:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Dec 2 13:25:26 2007 Subject: [Histonet] RE: Slide Quality In-Reply-To: <000701c83517$fc878940$1bf332d8@your4f1261a8e5> Message-ID: <291872.16288.qm@web61211.mail.yahoo.com> After many years struggling with this issue, including the usual pathologists' reluctance to review/grade slides, I came up with a method that satisfied everybody, and it was as follows: to let the pathologist REJECT slides (without being specific, just that it was below quality and could affect diagnosis). Those rejected slides were the ones I reviewed, graded, and discussed with the HT that prepared them. This constituted my QA and my training/evaluation tool. I worked fine for me. Other methods, like ramdomly selecting slides to review, took so much valuable time for me as manager/supervisor and rendered so few results (most of the slides were OK) and there were always slides I did not select to review that were rejected by the pathologists. Let the PT reject slides and deal with them afterwards. Ren? J. Susan Owens wrote: There should be a QC chart sent to the Pathologist daily with someone selecting a random H & E case..The Pathologist can check off such things as stain quality, wrinkles/folds etc....Poor sections should be addressed whether it interferes with the diagnosis or not..... There can be a problem within a certain section that makes it's early impossible for a wrinkle/fold fee selection...BUT, that would not be a daily issue...I would say any good Histo tech can and should have at least 99.% wrinkle/fold fee sections. If wrinkles/folds are routine, then that's a big problem that needs to be addressed and addressed now. >>> others input. I believe any time there is a wrinkle or fold it should >>> be >>> documented. I agree Sandy, BUT don't start a "witch hunt"...If you fine the poor quality sections coming from the same person(s) then a sit down talk with a walk down memory lane( as in back to basics) would be in order....Not a burning on the cross(at least not yet)... If wrinkles/folds are a big daily issue, then yes document, investigate and correct the problems..But if your talking about one wrinkle on one slide out of many slides.....then you can document, but don't go to war over it..... Susan > Message: 17 > Date: Fri, 30 Nov 2007 15:15:20 -0800 (PST) > From: Sandy Smith > Subject: RE: [Histonet] Slide quality > To: histonet@lists.utsouthwestern.edu > Message-ID: <648380.1758.qm@web57412.mail.re1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > When should the pathologist document wrinkles/folds? Only when it > interferes with the diagnosis? Or any time there is a wrinkle or fold? > Also how many histo techs out there get 100% wrinkle free sections? I > have > had discussions with my pathologists about these issues and would like > others input. I believe any time there is a wrinkle or fold it should be > documented. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get easy, one-click access to your favorites. Make Yahoo! your homepage. From amosbrooks <@t> gmail.com Sun Dec 2 20:03:40 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Dec 2 20:03:46 2007 Subject: [Histonet] Path from accross the pond Message-ID: <582736990712021803y684c68b2o76420b0e75d9c927@mail.gmail.com> Sorry Kelmo... I just couldn't resist that jab. It just lined up so perfectly Amos From Daivik_Shah <@t> rush.edu Sun Dec 2 20:41:38 2007 From: Daivik_Shah <@t> rush.edu (Daivik_Shah@rush.edu) Date: Sun Dec 2 20:41:53 2007 Subject: [Histonet] Problems in Neurofilaments-31 Staining Message-ID: Hi guys, We are right now working on Neurofilaments SMI-31 staining from Abcam laboratory on brain tissues. We had used 1:100, 1:500, 1:1000 dilutions of that stain. But still, it is very light. We had also did pre-treatment with citrate buffer and also with TBS buffer. Can anyone give suggestions about this staining? Do you have also some problem with this antibody or from same company? Thanks. Daivik ________________________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. From mariatere <@t> infovia.com.ar Sun Dec 2 21:22:08 2007 From: mariatere <@t> infovia.com.ar (Teresa Dominguez) Date: Sun Dec 2 21:22:12 2007 Subject: [Histonet] unsuscribe me Message-ID: <004801c8355b$b150d5a0$4e02fea9@tere> Please, unsuscribe me this time. From cateatmuse <@t> gmail.com Sun Dec 2 22:31:22 2007 From: cateatmuse <@t> gmail.com (Sydney Huang) Date: Sun Dec 2 22:31:26 2007 Subject: [Histonet] Trouble in staining the cytoplasma using Masson's trichrome Message-ID: Hi everyone, I am a student in Taiwan and currently studying the effects of nanoparticles on mice. Paraffin embedded lung section of mouse is used for the stain. I use the commercial Masson's trichrome kit (H15) from Sigma-Aldrich for the stain and followed the manufacturer's instruction. But the problem is that the cytoplasma showed little or no staining at all. So I tried to extend the time of Biebrich Scarlet upt to 30min but it showed no improvement. Can someone give me a hint what might happened? If the chemicals are just expired (may) will it affect the results? From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Dec 3 02:09:12 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Dec 3 02:09:19 2007 Subject: [Histonet] Path from accross the pond Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2A2@wahtntex2.waht.swest.nhs.uk> No problemo Amos; I was getting really worried over the Americans lack of English sarcasm; never apologise for a good example but cherish it as it is so rare from that side of the pond. As is good wrinkle free flat well stained sections apparently . Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Andrew.Prior <@t> Smith-Nephew.com Mon Dec 3 02:43:14 2007 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Mon Dec 3 02:43:29 2007 Subject: [Histonet] RE:Slide quality -wrinkles In-Reply-To: <20071202180153.CBD16208DFC6@spam.smith-nephew.com> References: <20071202180153.CBD16208DFC6@spam.smith-nephew.com> Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF370450B91F@EHS021.wound.san> I picked up this useful tip at the NSH conference in Denver. Thanks to whoever it was that suggested that tip. It's been a godsend these last two weeks cutting tricky bone samples. Once you pick up your section from the waterbath, place on a hotplate at 70deg C for 10-15 seconds before moving to your normal drying set-up. This brief heating is enough to melt the wax and most small wrinkles disappear. I've not seen any negative effect on the tissue quality or staining, just nice smooth sections. Of course you should check if the problems are with processing or a specific tech, but sometimes the tissue doesn't want to cut nicely and this helps. Andrew Prior Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From settembr <@t> umdnj.edu Mon Dec 3 06:18:39 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Dec 3 06:19:06 2007 Subject: [Histonet] CDX2 and HCA (hepatocellular carcinoma) Message-ID: I use Novocastra's CDX2 and I use Dako's Anti-Huan Hepatocyte, or Hep Par 1, or HCA Hope that helps. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Piche-Grocki, Jessica" 11/30/07 12:33 PM >>> Hello, I am having a hard time finding information for our pathologists on CDX2 and HCA antibodies. If anyone has any information they can share that would be great. I am especially having a hard time with the HCA(hepatocellular carcinoma antigen). Thanks and have a great weekend!! Jessica Piche-Grocki, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Mon Dec 3 06:16:53 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Dec 3 06:19:07 2007 Subject: [Histonet] MSI testing Message-ID: What vendors are people using for MSI testing? I am looking at: MLH-1 MSH-2 MSH-6 and PMS2 Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From relia1 <@t> earthlink.net Mon Dec 3 07:31:19 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Dec 3 07:31:25 2007 Subject: [Histonet] Junior Histotech - Opportunity to learn MOHS! -- Los Angeles near UCLA Message-ID: Hi Histonetters! I hope everyone had a great weekend. I am working with a dermpath group in Los Angeles that is looking for a full time person to work in their office doing MOHS and some medical assisting. They are looking for either an entry level or junior tech with basic histology skills that they can train in MOHS. This is a full time position and the hours are Monday thru Friday 8-5. At this point I think they prefer a local candidate. ASCP eligibility is not an issue for them. They offer excellent benefits and again the opportunity to learn Mohs. If you are interested or know someone who might be please contact me at relia1@earthlink.net or toll free at 866-607-3542. The position is open for someone to start immediately or after the holidays whichever is more convenient. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From rjbuesa <@t> yahoo.com Mon Dec 3 07:32:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 3 07:32:15 2007 Subject: [Histonet] Trouble in staining the cytoplasma using Masson's trichrome In-Reply-To: Message-ID: <776350.51484.qm@web61221.mail.yahoo.com> Probably your kit is old (expired) because it is very difficult NOT tostain with Biebrich Scarlet, it always work. Get a new kit or prepare your solutions yourself. Ren? J. Sydney Huang wrote: Hi everyone, I am a student in Taiwan and currently studying the effects of nanoparticles on mice. Paraffin embedded lung section of mouse is used for the stain. I use the commercial Masson's trichrome kit (H15) from Sigma-Aldrich for the stain and followed the manufacturer's instruction. But the problem is that the cytoplasma showed little or no staining at all. So I tried to extend the time of Biebrich Scarlet upt to 30min but it showed no improvement. Can someone give me a hint what might happened? If the chemicals are just expired (may) will it affect the results? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. From JWEEMS <@t> sjha.org Mon Dec 3 07:47:33 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Dec 3 07:47:57 2007 Subject: [Histonet] MSI testing In-Reply-To: References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F43BA@sjhaexc02.sjha.org> I use Genzyme -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Monday, December 03, 2007 7:17 AM To: histonet@lists.utsouthwestern.edu; Jessica Piche-Grocki Subject: [Histonet] MSI testing What vendors are people using for MSI testing? I am looking at: MLH-1 MSH-2 MSH-6 and PMS2 Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From cmiller <@t> physlab.com Mon Dec 3 08:33:49 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Dec 3 08:33:50 2007 Subject: [Histonet] RE: Slide Quality In-Reply-To: <000701c83517$fc878940$1bf332d8@your4f1261a8e5> References: <000701c83517$fc878940$1bf332d8@your4f1261a8e5> Message-ID: <010201c835b9$84dd0bc0$3402a8c0@plab.local> I agree with everything you have said. If it is a trend with a particular tech then obviously you address that. We also have a Daily QC that the path grades on total slide quality, processing, embedding, sectioning, staining, cover slipping and labeling. Isn't that the purpose of the daily QC sheet? The question was who gets 100% wrinkle and folds free. I was saying to get that one perfect slide , one might cut away tissue if it was a difficult block....sometimes its better to get "the best" section you can. If it's not a trend with a tech why document it?? The path has already done so in the daily QC haven't they?? Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Sunday, December 02, 2007 1:18 PM To: Histonet Subject: [Histonet] RE: Slide Quality There should be a QC chart sent to the Pathologist daily with someone selecting a random H & E case..The Pathologist can check off such things as stain quality, wrinkles/folds etc....Poor sections should be addressed whether it interferes with the diagnosis or not..... There can be a problem within a certain section that makes it's early impossible for a wrinkle/fold fee selection...BUT, that would not be a daily issue...I would say any good Histo tech can and should have at least 99.% wrinkle/fold fee sections. If wrinkles/folds are routine, then that's a big problem that needs to be addressed and addressed now. >>> others input. I believe any time there is a wrinkle or fold it should >>> be >>> documented. I agree Sandy, BUT don't start a "witch hunt"...If you fine the poor quality sections coming from the same person(s) then a sit down talk with a walk down memory lane( as in back to basics) would be in order....Not a burning on the cross(at least not yet)... If wrinkles/folds are a big daily issue, then yes document, investigate and correct the problems..But if your talking about one wrinkle on one slide out of many slides.....then you can document, but don't go to war over it..... Susan > Message: 17 > Date: Fri, 30 Nov 2007 15:15:20 -0800 (PST) > From: Sandy Smith > Subject: RE: [Histonet] Slide quality > To: histonet@lists.utsouthwestern.edu > Message-ID: <648380.1758.qm@web57412.mail.re1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > When should the pathologist document wrinkles/folds? Only when it > interferes with the diagnosis? Or any time there is a wrinkle or fold? > Also how many histo techs out there get 100% wrinkle free sections? I > have > had discussions with my pathologists about these issues and would like > others input. I believe any time there is a wrinkle or fold it should be > documented. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From talulahgosh <@t> gmail.com Mon Dec 3 09:10:41 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Dec 3 09:10:46 2007 Subject: [Histonet] unsuscribe me In-Reply-To: <004801c8355b$b150d5a0$4e02fea9@tere> References: <004801c8355b$b150d5a0$4e02fea9@tere> Message-ID: ATTENTION! Click on the link in the emails to unsubscribe. DO NOT WRITE TO THE LIST. On Dec 2, 2007 10:22 PM, Teresa Dominguez wrote: > Please, unsuscribe me this time. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- crow tom mike and joel trapped in space the endless void we've got movie sign! mst3k haiku brought to you by joe wiencis From gu.lang <@t> gmx.at Mon Dec 3 10:38:05 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Dec 3 10:38:18 2007 Subject: AW: [Histonet] Trouble in staining the cytoplasma using Masson'strichrome In-Reply-To: Message-ID: <004501c835ca$e1e3d590$6412a8c0@dielangs.at> Check the pH of the Biebrich Scarlet. It should be around 2,5. Prolong the PMA/PTA-step, that enhances the cytoplasma-staining and decolorize the collagen-fibers. How long was the tissue fixed? What fixative? - Fixing the deparaffinized slides 1-2 hours in Bouin at 60?C, or overnight at roomtemperature should emprove the staining. Very long (months) fixation in formaldehyd could cause a weak cytoplasma staining. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sydney Huang Gesendet: Montag, 03. Dezember 2007 05:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Trouble in staining the cytoplasma using Masson'strichrome Hi everyone, I am a student in Taiwan and currently studying the effects of nanoparticles on mice. Paraffin embedded lung section of mouse is used for the stain. I use the commercial Masson's trichrome kit (H15) from Sigma-Aldrich for the stain and followed the manufacturer's instruction. But the problem is that the cytoplasma showed little or no staining at all. So I tried to extend the time of Biebrich Scarlet upt to 30min but it showed no improvement. Can someone give me a hint what might happened? If the chemicals are just expired (may) will it affect the results? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Mon Dec 3 12:14:35 2007 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Mon Dec 3 12:14:41 2007 Subject: [Histonet] Florida licensure question Message-ID: <4754478B.6010407@gmail.com> I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth From ROrr <@t> enh.org Mon Dec 3 13:17:59 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Dec 3 13:18:06 2007 Subject: [Histonet] cardboard storage boxes Message-ID: Hi Everyone, I am in search of a specific type of storage box. It's a cardboard box that holds 100 scintillation bottles. Square 12" x 12" x 2.5" H I don't need the bottles; I just need the box that has the 100 spaced dividers (empty box) I have asked help from the manufacturer of the bottles as well as the actual box supplier, alas, to no avail. If anyone has this type of box I would be very appreciative and would remember you fondly in my last will and testament. Please let me know and I'll arrange to have it sent to me. And don't ask me why I need it, it's a long long boring story. Thanks Bec Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From carl.hobbs <@t> kcl.ac.uk Mon Dec 3 14:13:17 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Dec 3 14:14:02 2007 Subject: [Histonet] re:Problems in Neurofilaments-31 Staining Message-ID: <005701c835e8$f1b00a70$4101a8c0@carlba65530bda> Hi. If you want to demonstrate NFs in general, I am surprised that you have chosen to test an Ab reagent that has not been validated for pwax sections, by Abcam. It may well be that this SMI31 clone is not pwax-reactive. If I am wrong..my apologies. Sigma's anti NF 68 (N-5139), NF160 ( N-5264), NF200 ( N-0142) alone or in a cocktail are very good. Also, "RMO" clone (NF160) and RT97 clone ( NF200) are superb Ab reagents. Imho, these are the "standards" for Neurofilament detection. Carl From Ronald.Houston <@t> nationwidechildrens.org Mon Dec 3 14:31:05 2007 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Dec 3 14:31:41 2007 Subject: [Histonet] SMI-31 phosphorylated neurofilament Message-ID: <979FF5962E234F45B06CF0DB7C1AABB213B9CAE6@chi2k3ms01.columbuschildrens.net> Sorry deleted original message so do not know who posted the query but use the antibody from Covance, cat # SMI-31R; excellent results with 1:800 dilution after EDTA retrieval. No experience with this antibody from AbCam but have had good results with other antibodies from them. http://store.crpinc.com/pdfdatasheet.aspx?catalogno=SMI-31R Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org From FMonson <@t> wcupa.edu Mon Dec 3 14:38:31 2007 From: FMonson <@t> wcupa.edu (Monson, Frederick ) Date: Mon Dec 3 14:38:39 2007 Subject: [Histonet] RE: Retic jargon In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09DC4A@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C0A09DC4A@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A42F@wcu-ex-emp2.PASSHE.LCL> Maresch (1905) was an old dead guy when I was a young guy in 1965 and working with the "Silver Impregnation for Retiuclin" - by Pearse (1968) out of Maresch? Book is gone, and I'm just lingering. Two historic points. 1. The above impregnation - of only meager vicarious value - 'showed' fine fibers/fibrils around cells (especially in hematopoietic tissue) that were generally unstained by other prevailing methods, AND did NOT 'color' collagen except to a 'light tan'. 2. Even though Watson and Crick presented the model for DNA in 1953, it did not inhabit many undergraduate classrooms until the mid-1960's - acknowledging exceptions such as Havahd and Yail. Thus, even though silver impregnation of reticular fibers, reticulin or a reticulum is still performed, the procedure does not beat the MAb for Collagen III. Although, I must add, a notable MAb for an (human) elastin epitope proved conclusively (and negatively!) in the mid 1990's that the rabbit lacked elastin in its urinary bladder despite proof to the contrary from a widely accepted Gomori's Aldehyde Fuchsin stain. I still say, if we just stay focused on the significant surgical importance of dissection vs. disection, we will be far better off in the long run. We absolutely must dissuade young medical students from either enunciating or doing dIsections. In the domain of the physical sciences, we must pass a law that prevents anyone who can't pronounce the word 'NUCLEAR', from ever purchasing or having access to a red telephone. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Microanalysis and Imaging Research and Training Center (MIRTC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl Web Page: http://lexspiac.wcupa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, November 30, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Retic jargon We say "Reticulum" ??? The term reticulin was coined in 1892 by M. Siegfried.[2] Today the term reticulin or reticular fiber is restricted to fibers composed of type III collagen . However, during the pre-molecular era, there was confusion in the use of the term 'reticulin', which was used to describe two structures: * the argyrophilic (silver staining) fibrous structures present in basement membranes * histologically similar fibers present in developing connective tissue[3] . The history of the reticulin silver stain is reviewed by Puchtler et al. (1978).[4] The abstract of this paper says: Maresch (1905) introduced Bielschowsky's silver impregnation technic for neurofibrils as a stain for reticulum fibers, but emphasized the nonspecificity of such procedures. This lack of specificity has been confirmed repeatedly. Yet, since the 1920's the definition of "reticulin" and studies of its distribution were based solely on silver impregnation technics. The chemical mechanism and specificity of this group of stains is obscure. Application of Gomori's and Wilder's methods to human tissues showed variations of staining patterns with the fixatives and technics employed. Besides reticulum fibers, various other tissue structures, e.g. I bands of striated muscle, fibers in nervous tissues, and model substances, e.g. polysaccharides, egg white, gliadin, were also stained. Deposition of silver compounds on reticulum fibers was limited to an easily removable substance; the remaining collagen component did not bind silver. These histochemical studies indicate that silver impregnation technics for reticulum fibers have no chemical significance and cannot be considered as histochemical technics for "reticulin" or type III collagen. Who'd a thunk it? TGIF Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FMonson <@t> wcupa.edu Mon Dec 3 14:39:50 2007 From: FMonson <@t> wcupa.edu (Monson, Frederick ) Date: Mon Dec 3 14:40:02 2007 Subject: [Histonet] RE: Retic jargon - Wiki In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09DC4A@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C0A09DC4A@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A431@wcu-ex-emp2.PASSHE.LCL> Further, if we don't know more than is given on Wikipedia, we deserve to believe what is written there as Gospel. Frederick C. Monson, PhD Technical Director Microanalysis and Imaging Research and Training Center (MIRTC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl Web Page: http://lexspiac.wcupa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, November 30, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Retic jargon We say "Reticulum" ??? The term reticulin was coined in 1892 by M. Siegfried.[2] Today the term reticulin or reticular fiber is restricted to fibers composed of type III collagen . However, during the pre-molecular era, there was confusion in the use of the term 'reticulin', which was used to describe two structures: * the argyrophilic (silver staining) fibrous structures present in basement membranes * histologically similar fibers present in developing connective tissue[3] . The history of the reticulin silver stain is reviewed by Puchtler et al. (1978).[4] The abstract of this paper says: Maresch (1905) introduced Bielschowsky's silver impregnation technic for neurofibrils as a stain for reticulum fibers, but emphasized the nonspecificity of such procedures. This lack of specificity has been confirmed repeatedly. Yet, since the 1920's the definition of "reticulin" and studies of its distribution were based solely on silver impregnation technics. The chemical mechanism and specificity of this group of stains is obscure. Application of Gomori's and Wilder's methods to human tissues showed variations of staining patterns with the fixatives and technics employed. Besides reticulum fibers, various other tissue structures, e.g. I bands of striated muscle, fibers in nervous tissues, and model substances, e.g. polysaccharides, egg white, gliadin, were also stained. Deposition of silver compounds on reticulum fibers was limited to an easily removable substance; the remaining collagen component did not bind silver. These histochemical studies indicate that silver impregnation technics for reticulum fibers have no chemical significance and cannot be considered as histochemical technics for "reticulin" or type III collagen. Who'd a thunk it? TGIF Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Mon Dec 3 15:11:28 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Dec 3 15:11:56 2007 Subject: [Histonet] CD26 In-Reply-To: <641CEFFC7E5B6C42AB59539653FD08230517A431@wcu-ex-emp2.PASSHE.LCL> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B7001@NVCIEXCH02.NVCI.org> Has anyone ever stained for CD26 in FFPE human tissue? If so, please share your experiences. I'm supposed to get this stain up and running for a clinical trial that is starting up and I'm not having much luck. The Japanese group we're working with is coming to town in a few weeks and I'm getting a little nervous. I need to stain in kidney and some other normal tissues, but I want to see it in tonsil first so I can really have some faith in the stain. I have several antibodies, including the humanized version that is the drug. The humanized antibody is biotinylated. Thanks, Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Jeannette.Mitchell <@t> vtmednet.org Mon Dec 3 15:26:50 2007 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Mon Dec 3 15:26:49 2007 Subject: [Histonet] H Pylori In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE011B7001@NVCIEXCH02.NVCI.org> References: <641CEFFC7E5B6C42AB59539653FD08230517A431@wcu-ex-emp2.PASSHE.LCL> <5AEC610C1CE02945BD63A395BA763EDE011B7001@NVCIEXCH02.NVCI.org> Message-ID: Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone?? Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05403 From rjbuesa <@t> yahoo.com Mon Dec 3 15:32:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 3 15:32:41 2007 Subject: [Histonet] H Pylori In-Reply-To: Message-ID: <601152.81805.qm@web61217.mail.yahoo.com> If you are using silver (Steiner) it can be the silver solution and the developer. Ren? J. "Mitchell, Jeannette M." wrote: Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone?? Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Mon Dec 3 15:33:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 3 15:33:41 2007 Subject: [Histonet] Florida licensure question In-Reply-To: <4754478B.6010407@gmail.com> Message-ID: <280640.93519.qm@web61223.mail.yahoo.com> E-mail the Florida Histotechnology Society. They are very helpful. Ren? J. Beth Cox wrote: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. From JMeade0710 <@t> aol.com Mon Dec 3 16:02:17 2007 From: JMeade0710 <@t> aol.com (JMeade0710@aol.com) Date: Mon Dec 3 16:11:44 2007 Subject: [Histonet] Florida licensure question Message-ID: I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) From rjbuesa <@t> yahoo.com Mon Dec 3 16:23:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 3 16:23:39 2007 Subject: [Histonet] Florida licensure question In-Reply-To: Message-ID: <778435.73033.qm@web61212.mail.yahoo.com> Same advise, e-mail the Florida Society for Histotechnologist. Ren? J. JMeade0710@aol.com wrote: I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From taben.hale <@t> gmail.com Mon Dec 3 16:25:55 2007 From: taben.hale <@t> gmail.com (Taben Hale) Date: Mon Dec 3 16:26:07 2007 Subject: [Histonet] Schiff's reagent Message-ID: What is the shelf-life and stability of Schiff's reagent? Can it be used multiple times? -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 From rjbuesa <@t> yahoo.com Mon Dec 3 16:29:41 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 3 16:29:53 2007 Subject: [Histonet] Schiff's reagent In-Reply-To: Message-ID: <453373.58567.qm@web61216.mail.yahoo.com> It has to be kept in the refrigerator, and yes, it can be used several times. You can test how is working by adding a few drops of the Schiff's reagent to NBF, it it reacts quickly with a magenta color, is working OK, if not, discard it. Ren? J. Taben Hale wrote: What is the shelf-life and stability of Schiff's reagent? Can it be used multiple times? -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. From algranth <@t> u.arizona.edu Mon Dec 3 16:50:16 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Dec 3 16:56:15 2007 Subject: [Histonet] uranium - question for microscope slide manufacturers possibly Message-ID: <6.2.3.4.1.20071203153611.01ef6358@algranth.inbox.email.arizona.edu> I have a question about slides containing concentrations of uranium. One of my investigators is working on a project where they are lasering tissue off of slides and checking it for the amount of uranium in the tissue. The laser actually vaporizes the tissue and it goes into a machine where it is tested for uranium content. They were getting some strange readings and tested the slides alone without tissue and found that the slides alone had a pretty high uranium content. So she is asking - Is there some coating to put on the slides to block the uranium? My question is this - is there really uranium in slides and just how much uranium is in a glass slide? Can this be dangerous? Are there some slides out there that do not contain uranium? I hope somebody has an answer for my weird question of the day. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From algranth <@t> u.arizona.edu Mon Dec 3 16:55:01 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Dec 3 17:00:59 2007 Subject: [Histonet] Schiff's reagent In-Reply-To: <453373.58567.qm@web61216.mail.yahoo.com> References: <453373.58567.qm@web61216.mail.yahoo.com> Message-ID: <6.2.3.4.1.20071203155105.01fc65a8@algranth.inbox.email.arizona.edu> I'm using a Schiff's from Newcomer Supply that has a pretty long shelf life. It can be stored at RT and can be used several times. We always put the "used" Schiff's into a different bottle as to not contaminate the unused solution. Andi Grantham At 03:29 PM 12/3/2007, Rene J Buesa wrote: >It has to be kept in the refrigerator, and yes, it can be used several times. > You can test how is working by adding a few > drops of the Schiff's reagent to NBF, it it > reacts quickly with a magenta color, is working OK, if not, discard it. > Ren? J. > >Taben Hale wrote: > What is the shelf-life and stability of Schiff's reagent? Can it be used >multiple times? > >-- >Taben M Hale, PhD >Postdoctoral Fellow >Universite de Montreal >Dept Pharmacologie >(514)343-6111 ext. 4968 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------- >Be a better sports nut! Let your teams follow >you with Yahoo Mobile. Try it now. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From tkngflght <@t> yahoo.com Mon Dec 3 16:32:45 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Mon Dec 3 18:22:52 2007 Subject: [Histonet] Schiff's reagent In-Reply-To: Message-ID: <00c701c835fc$6d8b3710$6901a8c0@CHERYLSLAPTOP> Hello Taben- The easiest way to determine Schiff's reactivity is to drip a little formalin into it. Should turn BRIGHT purple. Try it fresh and remember what it looked like for a baseline as it ages. If you're making it yourself (great fun--don't break the flask!!)--shelf life can vary. Keep it in the fridge and it'll last 6 mos to a year. It CAN be used multiple times but it will be less reactive each time. Many labs I know use it daily for two weeks at a time but you have to be careful to verify intensity with the control tissue. More consistent results are had by placing the slides on their backs and dropping about 2-5 ml on the tissue for the proscribed time, then rinsing in warm running water to develop the color and clean the slide before counterstaining. I'm sure you'll get a variety of responses--hope this one helps. Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taben Hale Sent: Monday, December 03, 2007 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Schiff's reagent What is the shelf-life and stability of Schiff's reagent? Can it be used multiple times? -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peddinti_2002us <@t> yahoo.co.in Mon Dec 3 19:14:17 2007 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Mon Dec 3 19:14:33 2007 Subject: [Histonet] massons trichrome Message-ID: <345369.95578.qm@web8703.mail.in.yahoo.com> Differentiate in phosphomolybdic-phosphotungstic acid solution for 15 minutes or until collagen and cytoplasm is not red. Transfer sections directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse briefly in distilled water and differentiate in 1% acetic acid solution for 2-5 minutes. Also I would suggest you to use another control to confirm(Fixation and Processing). Regards, --------------------------------- Why delete messages? Unlimited storage is just a click away. From kwuny <@t> email.cs.nsw.gov.au Mon Dec 3 21:20:56 2007 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Mon Dec 3 21:21:34 2007 Subject: [Histonet] Permanent Red stain Message-ID: <200712041420715.SM01624@csls2816> Dear Histonetters, One of my colleagues is having a problem with Dako's Permanent Liquid Red staining. She told me that she was getting a heavy background around the tissues. She is using DKDH's Untra-V-Block goat serum for blocking and the detection system is DKSH's ONE Alkaline Phosphatase Polymer. I use Dako's Fast Red Substrate system with Dako's labeled Polymer, AP (anti-mouse & anti-rabbit) without such problem. I was wondering what could cause such problems. I understand that Liquid Red is basically the same as the Fast Red. Is that true? Thank you in advance for your suggestion. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au From louise.renton <@t> gmail.com Tue Dec 4 01:09:12 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Dec 4 01:09:29 2007 Subject: [Histonet] uranium - question for microscope slide manufacturers possibly In-Reply-To: <6.2.3.4.1.20071203153611.01ef6358@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20071203153611.01ef6358@algranth.inbox.email.arizona.edu> Message-ID: This is absolute free thinking here....... could this be contamination of some sort? Perhaps the lasering process "kicks" off some cells containing U if they are not tightly bound to the slide and they float around to settle and upset everyones measurements Try testing slides form a new, unopened box perferably from another distant lab or dierctly from the supplier or another supplier altogether. What is the background U content in Arizona? Think of the example of arsenic in exhumed bodies. The amount found in the tissue can be rendered meaningless if the levels in the surrounding soil around the bodies is significant. recalibrate the equipment with a known standard and retest Just my 2 cents worth On 12/4/07, Andrea Grantham wrote: > > I have a question about slides containing concentrations of uranium. > > One of my investigators is working on a project where they are > lasering tissue off of slides and checking it for the amount of > uranium in the tissue. The laser actually vaporizes the tissue and it > goes into a machine where it is tested for uranium content. They were > getting some strange readings and tested the slides alone without > tissue and found that the slides alone had a pretty high uranium content. > > So she is asking - Is there some coating to put on the slides to > block the uranium? > > My question is this - is there really uranium in slides and just how > much uranium is in a glass slide? Can this be dangerous? > > Are there some slides out there that do not contain uranium? > > I hope somebody has an answer for my weird question of the day. > > Thanks. > > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Dec 4 06:04:11 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Dec 4 06:04:26 2007 Subject: [Histonet] Schiff's reagent Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2BA@wahtntex2.waht.swest.nhs.uk> Used to make this myself by bubbling SO2 through basic fuchsin, really good fun, but we can't do it now. The stock solution should be kept in the fridge and is OK when clear (if it starts to go pink discard). We used to satin up to 5 slides in a coplin jar which was stored in the fridge in between times. But then we just dripped a bit onto the section as it was felt it was better to waste small aliquots of the stain rather than risking the coplin jar contents becoming 'weak'. Schiff's was cheapish then as we made it ourselves but I guess it now costs a lot more made up? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From dawn_cowie <@t> yahoo.com Tue Dec 4 06:41:52 2007 From: dawn_cowie <@t> yahoo.com (Dawn Cowie) Date: Tue Dec 4 06:42:06 2007 Subject: [Histonet] Florida licensure question In-Reply-To: <778435.73033.qm@web61212.mail.yahoo.com> Message-ID: <668430.6557.qm@web45012.mail.sp1.yahoo.com> 2 companies that I have used. Florida Excel and Anderson Continuing Ed. They both sell CE materials and are recognised by state of Florida for obtaining your license. You can contact them both via internet. Dawn Rene J Buesa wrote: Same advise, e-mail the Florida Society for Histotechnologist. Ren? J. JMeade0710@aol.com wrote: I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get easy, one-click access to your favorites. Make Yahoo! your homepage. From rjbuesa <@t> yahoo.com Tue Dec 4 07:03:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 07:03:16 2007 Subject: [Histonet] uranium - question for microscope slide manufacturers possibly In-Reply-To: <6.2.3.4.1.20071203153611.01ef6358@algranth.inbox.email.arizona.edu> Message-ID: <919617.96798.qm@web61217.mail.yahoo.com> I don't think that any coating will "block" the uranium if it is present (a physics thing). Try to contact the manufacturer to find aout about the uranium contents of their raw (silicon) material. Ren? J. Andrea Grantham wrote: I have a question about slides containing concentrations of uranium. One of my investigators is working on a project where they are lasering tissue off of slides and checking it for the amount of uranium in the tissue. The laser actually vaporizes the tissue and it goes into a machine where it is tested for uranium content. They were getting some strange readings and tested the slides alone without tissue and found that the slides alone had a pretty high uranium content. So she is asking - Is there some coating to put on the slides to block the uranium? My question is this - is there really uranium in slides and just how much uranium is in a glass slide? Can this be dangerous? Are there some slides out there that do not contain uranium? I hope somebody has an answer for my weird question of the day. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. From rjbuesa <@t> yahoo.com Tue Dec 4 07:04:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 07:04:21 2007 Subject: [Histonet] Permanent Red stain In-Reply-To: <200712041420715.SM01624@csls2816> Message-ID: <327436.88165.qm@web61213.mail.yahoo.com> My advise is to contact DAKO. They are very cooperative and knowledgeable of their products. Ren? J. Young Kwun wrote: Dear Histonetters, One of my colleagues is having a problem with Dako's Permanent Liquid Red staining. She told me that she was getting a heavy background around the tissues. She is using DKDH's Untra-V-Block goat serum for blocking and the detection system is DKSH's ONE Alkaline Phosphatase Polymer. I use Dako's Fast Red Substrate system with Dako's labeled Polymer, AP (anti-mouse & anti-rabbit) without such problem. I was wondering what could cause such problems. I understand that Liquid Red is basically the same as the Fast Red. Is that true? Thank you in advance for your suggestion. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. From GDawson <@t> dynacaremilwaukee.com Tue Dec 4 07:44:29 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Dec 4 07:44:44 2007 Subject: [Histonet] H Pylori In-Reply-To: Message-ID: Jeannette, We had a similar problem and solved it by switching to non-charged slides for our silver stains. It seems that the adhesive coating was taking up the stain. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mitchell, Jeannette M. Sent: Monday, December 03, 2007 3:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] H Pylori Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone?? Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Tue Dec 4 08:22:49 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Dec 4 08:23:02 2007 Subject: [Histonet] CD26 Message-ID: Has anyone ever stained for CD26 in FFPE human tissue? If so, please share your experiences. I'm supposed to get this stain up and running for a clinical trial that is starting up and I'm not having much luck. The Japanese group we're working with is coming to town in a few weeks and I'm getting a little nervous. I need to stain in kidney and some other normal tissues, but I want to see it in tonsil first so I can really have some faith in the stain. I have several antibodies, including the humanized version that is the drug. The humanized antibody is biotinylated. Thanks, Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 Mark, I just started working up CD26 this week, using the rabbit polyclonal catalog # sc9153 from Santa Cruz. The brief data in the 2006 catalog indicates this would have been the most appropriate antibody to use on FFPE tonsil. However, after I purchased the product, I went onto their website www.scbt.com and found that the description of this particular rabbit ab had changed and it was no longer recommended for FFPE. They did have a mouse ab that was described as working with FFPE. In hindsight, I would have most likely purchased the mouse ab first off with this information. My result from this rabbit ab is "decent" the stain is not as well defined as I'm used to seeing on CD T cell markers, I may ask them for a sample of the mouse just to see if that's the problem. I have results with a dilution range of 1:200 to 1:500 on tonsil (depends on how strong you'd like the staining) Dilute with pbs based diluent (biocare green) HIER was done with Reveal and Borg, (biocare) in a decloaker The Borg (hi pH) gave a more distinct stain but the reveal had better morphology.... Detection was Mach4/DAB (biocare) Hope this helps. Becky Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From karenadams <@t> comcast.net Tue Dec 4 09:01:46 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Tue Dec 4 09:01:59 2007 Subject: [Histonet] recording Bx pieces Message-ID: <120420071501.23363.47556BDA000DE29F00005B4322134843739C030E0B0E020A9D0E05@comcast.net> Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From Terry.Marshall <@t> rothgen.nhs.uk Tue Dec 4 09:16:55 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Dec 4 09:17:10 2007 Subject: [Histonet] recording Bx pieces Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AEA6@TRFT-EX01.xRothGen.nhs.uk> The question would have been better in plain English, but even then, is missing essential detail without which the question is unanswerable. Submitted to whom, by whom, in what time interval, and what is being regarded as a biopsy in this particular context? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: 04 December 2007 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Dec 4 09:26:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Dec 4 09:26:30 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <120420071501.23363.47556BDA000DE29F00005B4322134843739C030E0B0E020A9D0E05@comcast.net> References: <120420071501.23363.47556BDA000DE29F00005B4322134843739C030E0B0E020A9D0E05@comcast.net> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4402@sjhaexc02.sjha.org> Because you want to be able to account for all pieces when embedding. If you don't know how many you had to start with, you won't know how many to look for when embedding and how many are lost or flipped away, etc. The surgeon records the number of pieces and if more are submitted than are reviewed, it could be a big problem! That would be my reason.... Cheers! J -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: Tuesday, December 04, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Mark.Frei <@t> sial.com Tue Dec 4 09:42:55 2007 From: Mark.Frei <@t> sial.com (Mark Frei) Date: Tue Dec 4 09:43:31 2007 Subject: [Histonet] Re: Histonet Digest, Retic jargon In-Reply-To: <200712041348.lB4DmCBx029176@stlspfw1.sial.com> Message-ID: Just to throw a new wrinkle into the retic nomenclature, I was performing retic staining (reticulocytes using new methylene blue) in hematology decades before becoming involved in histology. Since I dealt with both disciplines, people talking about "retic" staining caused me some momentary confusion in the beginning. Mark Frei MT(ASCP) Sigma-Aldrich Corporation 3050 Spruce Street St. Louis, MO 63103 (314) 286-8080 Message: 5 Date: Mon, 3 Dec 2007 15:38:31 -0500 From: "Monson, Frederick " Subject: RE: [Histonet] RE: Retic jargon To: "Renko, Heather D." , Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A42F@wcu-ex-emp2.PASSHE.LCL> Content-Type: text/plain; charset="us-ascii" Maresch (1905) was an old dead guy when I was a young guy in 1965 and working with the "Silver Impregnation for Retiuclin" - by Pearse (1968) out of Maresch? Book is gone, and I'm just lingering. Two historic points. 1. The above impregnation - of only meager vicarious value - 'showed' fine fibers/fibrils around cells (especially in hematopoietic tissue) that were generally unstained by other prevailing methods, AND did NOT 'color' collagen except to a 'light tan'. 2. Even though Watson and Crick presented the model for DNA in 1953, it did not inhabit many undergraduate classrooms until the mid-1960's - acknowledging exceptions such as Havahd and Yail. Thus, even though silver impregnation of reticular fibers, reticulin or a reticulum is still performed, the procedure does not beat the MAb for Collagen III. Although, I must add, a notable MAb for an (human) elastin epitope proved conclusively (and negatively!) in the mid 1990's that the rabbit lacked elastin in its urinary bladder despite proof to the contrary from a widely accepted Gomori's Aldehyde Fuchsin stain. I still say, if we just stay focused on the significant surgical importance of dissection vs. disection, we will be far better off in the long run. We absolutely must dissuade young medical students from either enunciating or doing dIsections. In the domain of the physical sciences, we must pass a law that prevents anyone who can't pronounce the word 'NUCLEAR', from ever purchasing or having access to a red telephone. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Microanalysis and Imaging Research and Training Center (MIRTC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl Web Page: http://lexspiac.wcupa.edu This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From rjbuesa <@t> yahoo.com Tue Dec 4 09:44:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 09:44:51 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <120420071501.23363.47556BDA000DE29F00005B4322134843739C030E0B0E020A9D0E05@comcast.net> Message-ID: <953583.62768.qm@web61223.mail.yahoo.com> Because that is the only objective way you have to determine if you processed them all! Ren? J. karenadams@comcast.net wrote: Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From eileen_dusek <@t> yahoo.com Tue Dec 4 10:03:37 2007 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Tue Dec 4 10:03:47 2007 Subject: [Histonet] fresh kidney bx Message-ID: <314881.98862.qm@web50610.mail.re2.yahoo.com> Hi Everyone, I have been in Histology for a looong time and have met my match. I recently have new duties involving kidney bxs. We receive a formalin, EM, and Michels (Zuess) fixed specimens. The Formalin and EM bx are not an issue, the Michels is kicking by butt! I transfer the specimen from Michels to PBS, to rinse the fixative, for about 2-3 mins of agitation. After the PBS the specimen goes into the first OCT. This is swirled for another 2-3mins. I repeat this process again. My problem is I get specimens that have too much freezing artifact. The structures are "blown up" and not compact. I appreciate any suggestions to help the problem Thanks Eileen Dusek --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Sheri.Meilus <@t> va.gov Tue Dec 4 10:10:00 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Tue Dec 4 10:10:18 2007 Subject: [Histonet] RE: Florida licensure question In-Reply-To: <6br0om$a32ub@mtadal2.dal.net.va.gov> References: <6br0om$a32ub@mtadal2.dal.net.va.gov> Message-ID: Anderson Continuing Education offers a 2 hour course on Medical Errors. It's called Prevention of Medical Errors 2007. Their web site is www.andersonCE.com They are recognized by Florida as a provider for CEUS. Relatively inexpensive too. S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, December 04, 2007 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 49, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Florida licensure question (Beth Cox) 2. cardboard storage boxes (Orr, Rebecca) 3. re:Problems in Neurofilaments-31 Staining (Carl Hobbs) 4. SMI-31 phosphorylated neurofilament (Houston, Ronald) 5. RE: RE: Retic jargon (Monson, Frederick ) 6. RE: RE: Retic jargon - Wiki (Monson, Frederick ) 7. CD26 (Tarango, Mark) 8. H Pylori (Mitchell, Jeannette M.) 9. Re: H Pylori (Rene J Buesa) 10. Re: Florida licensure question (Rene J Buesa) 11. Re: Florida licensure question (JMeade0710@aol.com) 12. Re: Florida licensure question (Rene J Buesa) 13. Schiff's reagent (Taben Hale) 14. Re: Schiff's reagent (Rene J Buesa) 15. uranium - question for microscope slide manufacturers possibly (Andrea Grantham) 16. Re: Schiff's reagent (Andrea Grantham) 17. RE: Schiff's reagent (Cheryl R. Kerry) 18. massons trichrome (kamal prasad) 19. Permanent Red stain (Young Kwun) 20. Re: uranium - question for microscope slide manufacturers possibly (louise renton) 21. RE: Schiff's reagent (Kemlo Rogerson) 22. Re: Florida licensure question (Dawn Cowie) 23. Re: uranium - question for microscope slide manufacturers possibly (Rene J Buesa) 24. Re: Permanent Red stain (Rene J Buesa) 25. RE: H Pylori (Dawson, Glen) ---------------------------------------------------------------------- Message: 1 Date: Mon, 03 Dec 2007 13:14:35 -0500 From: Beth Cox Subject: [Histonet] Florida licensure question To: histonet@lists.utsouthwestern.edu Message-ID: <4754478B.6010407@gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth ------------------------------ Message: 2 Date: Mon, 3 Dec 2007 13:17:59 -0600 From: "Orr, Rebecca" Subject: [Histonet] cardboard storage boxes To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Everyone, I am in search of a specific type of storage box. It's a cardboard box that holds 100 scintillation bottles. Square 12" x 12" x 2.5" H I don't need the bottles; I just need the box that has the 100 spaced dividers (empty box) I have asked help from the manufacturer of the bottles as well as the actual box supplier, alas, to no avail. If anyone has this type of box I would be very appreciative and would remember you fondly in my last will and testament. Please let me know and I'll arrange to have it sent to me. And don't ask me why I need it, it's a long long boring story. Thanks Bec Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 3 Date: Mon, 3 Dec 2007 20:13:17 -0000 From: "Carl Hobbs" Subject: [Histonet] re:Problems in Neurofilaments-31 Staining To: "Histonet" Message-ID: <005701c835e8$f1b00a70$4101a8c0@carlba65530bda> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi. If you want to demonstrate NFs in general, I am surprised that you have chosen to test an Ab reagent that has not been validated for pwax sections, by Abcam. It may well be that this SMI31 clone is not pwax-reactive. If I am wrong..my apologies. Sigma's anti NF 68 (N-5139), NF160 ( N-5264), NF200 ( N-0142) alone or in a cocktail are very good. Also, "RMO" clone (NF160) and RT97 clone ( NF200) are superb Ab reagents. Imho, these are the "standards" for Neurofilament detection. Carl ------------------------------ Message: 4 Date: Mon, 3 Dec 2007 15:31:05 -0500 From: "Houston, Ronald" Subject: [Histonet] SMI-31 phosphorylated neurofilament To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB213B9CAE6@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Sorry deleted original message so do not know who posted the query but use the antibody from Covance, cat # SMI-31R; excellent results with 1:800 dilution after EDTA retrieval. No experience with this antibody from AbCam but have had good results with other antibodies from them. http://store.crpinc.com/pdfdatasheet.aspx?catalogno=SMI-31R Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org ------------------------------ Message: 5 Date: Mon, 3 Dec 2007 15:38:31 -0500 From: "Monson, Frederick " Subject: RE: [Histonet] RE: Retic jargon To: "Renko, Heather D." , Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A42F@wcu-ex-emp2.PASSHE.LCL> Content-Type: text/plain; charset="us-ascii" Maresch (1905) was an old dead guy when I was a young guy in 1965 and working with the "Silver Impregnation for Retiuclin" - by Pearse (1968) out of Maresch? Book is gone, and I'm just lingering. Two historic points. 1. The above impregnation - of only meager vicarious value - 'showed' fine fibers/fibrils around cells (especially in hematopoietic tissue) that were generally unstained by other prevailing methods, AND did NOT 'color' collagen except to a 'light tan'. 2. Even though Watson and Crick presented the model for DNA in 1953, it did not inhabit many undergraduate classrooms until the mid-1960's - acknowledging exceptions such as Havahd and Yail. Thus, even though silver impregnation of reticular fibers, reticulin or a reticulum is still performed, the procedure does not beat the MAb for Collagen III. Although, I must add, a notable MAb for an (human) elastin epitope proved conclusively (and negatively!) in the mid 1990's that the rabbit lacked elastin in its urinary bladder despite proof to the contrary from a widely accepted Gomori's Aldehyde Fuchsin stain. I still say, if we just stay focused on the significant surgical importance of dissection vs. disection, we will be far better off in the long run. We absolutely must dissuade young medical students from either enunciating or doing dIsections. In the domain of the physical sciences, we must pass a law that prevents anyone who can't pronounce the word 'NUCLEAR', from ever purchasing or having access to a red telephone. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Microanalysis and Imaging Research and Training Center (MIRTC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl Web Page: http://lexspiac.wcupa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, November 30, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Retic jargon We say "Reticulum" ??? The term reticulin was coined in 1892 by M. Siegfried.[2] Today the term reticulin or reticular fiber is restricted to fibers composed of type III collagen . However, during the pre-molecular era, there was confusion in the use of the term 'reticulin', which was used to describe two structures: * the argyrophilic (silver staining) fibrous structures present in basement membranes * histologically similar fibers present in developing connective tissue[3] . The history of the reticulin silver stain is reviewed by Puchtler et al. (1978).[4] The abstract of this paper says: Maresch (1905) introduced Bielschowsky's silver impregnation technic for neurofibrils as a stain for reticulum fibers, but emphasized the nonspecificity of such procedures. This lack of specificity has been confirmed repeatedly. Yet, since the 1920's the definition of "reticulin" and studies of its distribution were based solely on silver impregnation technics. The chemical mechanism and specificity of this group of stains is obscure. Application of Gomori's and Wilder's methods to human tissues showed variations of staining patterns with the fixatives and technics employed. Besides reticulum fibers, various other tissue structures, e.g. I bands of striated muscle, fibers in nervous tissues, and model substances, e.g. polysaccharides, egg white, gliadin, were also stained. Deposition of silver compounds on reticulum fibers was limited to an easily removable substance; the remaining collagen component did not bind silver. These histochemical studies indicate that silver impregnation technics for reticulum fibers have no chemical significance and cannot be considered as histochemical technics for "reticulin" or type III collagen. Who'd a thunk it? TGIF Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 3 Dec 2007 15:39:50 -0500 From: "Monson, Frederick " Subject: RE: [Histonet] RE: Retic jargon - Wiki To: "Renko, Heather D." , Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A431@wcu-ex-emp2.PASSHE.LCL> Content-Type: text/plain; charset="us-ascii" Further, if we don't know more than is given on Wikipedia, we deserve to believe what is written there as Gospel. Frederick C. Monson, PhD Technical Director Microanalysis and Imaging Research and Training Center (MIRTC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl Web Page: http://lexspiac.wcupa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, November 30, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Retic jargon We say "Reticulum" ??? The term reticulin was coined in 1892 by M. Siegfried.[2] Today the term reticulin or reticular fiber is restricted to fibers composed of type III collagen . However, during the pre-molecular era, there was confusion in the use of the term 'reticulin', which was used to describe two structures: * the argyrophilic (silver staining) fibrous structures present in basement membranes * histologically similar fibers present in developing connective tissue[3] . The history of the reticulin silver stain is reviewed by Puchtler et al. (1978).[4] The abstract of this paper says: Maresch (1905) introduced Bielschowsky's silver impregnation technic for neurofibrils as a stain for reticulum fibers, but emphasized the nonspecificity of such procedures. This lack of specificity has been confirmed repeatedly. Yet, since the 1920's the definition of "reticulin" and studies of its distribution were based solely on silver impregnation technics. The chemical mechanism and specificity of this group of stains is obscure. Application of Gomori's and Wilder's methods to human tissues showed variations of staining patterns with the fixatives and technics employed. Besides reticulum fibers, various other tissue structures, e.g. I bands of striated muscle, fibers in nervous tissues, and model substances, e.g. polysaccharides, egg white, gliadin, were also stained. Deposition of silver compounds on reticulum fibers was limited to an easily removable substance; the remaining collagen component did not bind silver. These histochemical studies indicate that silver impregnation technics for reticulum fibers have no chemical significance and cannot be considered as histochemical technics for "reticulin" or type III collagen. Who'd a thunk it? TGIF Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 3 Dec 2007 13:11:28 -0800 From: "Tarango, Mark" Subject: [Histonet] CD26 To: histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B7001@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii Has anyone ever stained for CD26 in FFPE human tissue? If so, please share your experiences. I'm supposed to get this stain up and running for a clinical trial that is starting up and I'm not having much luck. The Japanese group we're working with is coming to town in a few weeks and I'm getting a little nervous. I need to stain in kidney and some other normal tissues, but I want to see it in tonsil first so I can really have some faith in the stain. I have several antibodies, including the humanized version that is the drug. The humanized antibody is biotinylated. Thanks, Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 8 Date: Mon, 3 Dec 2007 16:26:50 -0500 From: "Mitchell, Jeannette M." Subject: [Histonet] H Pylori To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone?? Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05403 ------------------------------ Message: 9 Date: Mon, 3 Dec 2007 13:32:28 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] H Pylori To: "Mitchell, Jeannette M." , "'histonet@lists.utsouthwestern.edu'" Message-ID: <601152.81805.qm@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If you are using silver (Steiner) it can be the silver solution and the developer. Ren? J. "Mitchell, Jeannette M." wrote: Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone?? Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ Message: 10 Date: Mon, 3 Dec 2007 13:33:27 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Florida licensure question To: Beth Cox , histonet@lists.utsouthwestern.edu Message-ID: <280640.93519.qm@web61223.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 E-mail the Florida Histotechnology Society. They are very helpful. Ren? J. Beth Cox wrote: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. ------------------------------ Message: 11 Date: Mon, 3 Dec 2007 17:02:17 EST From: JMeade0710@aol.com Subject: Re: [Histonet] Florida licensure question To: bethcoxx@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) ------------------------------ Message: 12 Date: Mon, 3 Dec 2007 14:23:26 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Florida licensure question To: JMeade0710@aol.com, bethcoxx@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: <778435.73033.qm@web61212.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Same advise, e-mail the Florida Society for Histotechnologist. Ren? J. JMeade0710@aol.com wrote: I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 13 Date: Mon, 3 Dec 2007 17:25:55 -0500 From: "Taben Hale" Subject: [Histonet] Schiff's reagent To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 What is the shelf-life and stability of Schiff's reagent? Can it be used multiple times? -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 ------------------------------ Message: 14 Date: Mon, 3 Dec 2007 14:29:41 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Schiff's reagent To: Taben Hale , histonet@lists.utsouthwestern.edu Message-ID: <453373.58567.qm@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It has to be kept in the refrigerator, and yes, it can be used several times. You can test how is working by adding a few drops of the Schiff's reagent to NBF, it it reacts quickly with a magenta color, is working OK, if not, discard it. Ren? J. Taben Hale wrote: What is the shelf-life and stability of Schiff's reagent? Can it be used multiple times? -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. ------------------------------ Message: 15 Date: Mon, 03 Dec 2007 15:50:16 -0700 From: Andrea Grantham Subject: [Histonet] uranium - question for microscope slide manufacturers possibly To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.1.20071203153611.01ef6358@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I have a question about slides containing concentrations of uranium. One of my investigators is working on a project where they are lasering tissue off of slides and checking it for the amount of uranium in the tissue. The laser actually vaporizes the tissue and it goes into a machine where it is tested for uranium content. They were getting some strange readings and tested the slides alone without tissue and found that the slides alone had a pretty high uranium content. So she is asking - Is there some coating to put on the slides to block the uranium? My question is this - is there really uranium in slides and just how much uranium is in a glass slide? Can this be dangerous? Are there some slides out there that do not contain uranium? I hope somebody has an answer for my weird question of the day. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 16 Date: Mon, 03 Dec 2007 15:55:01 -0700 From: Andrea Grantham Subject: Re: [Histonet] Schiff's reagent To: Rene J Buesa ,Taben Hale , histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.1.20071203155105.01fc65a8@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed I'm using a Schiff's from Newcomer Supply that has a pretty long shelf life. It can be stored at RT and can be used several times. We always put the "used" Schiff's into a different bottle as to not contaminate the unused solution. Andi Grantham At 03:29 PM 12/3/2007, Rene J Buesa wrote: >It has to be kept in the refrigerator, and yes, it can be used several times. > You can test how is working by adding a few > drops of the Schiff's reagent to NBF, it it > reacts quickly with a magenta color, is working OK, if not, discard it. > Ren? J. > >Taben Hale wrote: > What is the shelf-life and stability of Schiff's reagent? Can it be used >multiple times? > >-- >Taben M Hale, PhD >Postdoctoral Fellow >Universite de Montreal >Dept Pharmacologie >(514)343-6111 ext. 4968 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------- >Be a better sports nut! Let your teams follow >you with Yahoo Mobile. Try it now. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 17 Date: Mon, 3 Dec 2007 16:32:45 -0600 From: "Cheryl R. Kerry" Subject: RE: [Histonet] Schiff's reagent To: Message-ID: <00c701c835fc$6d8b3710$6901a8c0@CHERYLSLAPTOP> Content-Type: text/plain; charset="US-ASCII" Hello Taben- The easiest way to determine Schiff's reactivity is to drip a little formalin into it. Should turn BRIGHT purple. Try it fresh and remember what it looked like for a baseline as it ages. If you're making it yourself (great fun--don't break the flask!!)--shelf life can vary. Keep it in the fridge and it'll last 6 mos to a year. It CAN be used multiple times but it will be less reactive each time. Many labs I know use it daily for two weeks at a time but you have to be careful to verify intensity with the control tissue. More consistent results are had by placing the slides on their backs and dropping about 2-5 ml on the tissue for the proscribed time, then rinsing in warm running water to develop the color and clean the slide before counterstaining. I'm sure you'll get a variety of responses--hope this one helps. Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taben Hale Sent: Monday, December 03, 2007 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Schiff's reagent What is the shelf-life and stability of Schiff's reagent? Can it be used multiple times? -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 4 Dec 2007 01:14:17 +0000 (GMT) From: kamal prasad Subject: [Histonet] massons trichrome To: histonet@lists.utsouthwestern.edu Message-ID: <345369.95578.qm@web8703.mail.in.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Differentiate in phosphomolybdic-phosphotungstic acid solution for 15 minutes or until collagen and cytoplasm is not red. Transfer sections directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse briefly in distilled water and differentiate in 1% acetic acid solution for 2-5 minutes. Also I would suggest you to use another control to confirm(Fixation and Processing). Regards, --------------------------------- Why delete messages? Unlimited storage is just a click away. ------------------------------ Message: 19 Date: Tue, 4 Dec 2007 14:20:56 +1100 From: "Young Kwun" Subject: [Histonet] Permanent Red stain To: "Histonet" Message-ID: <200712041420715.SM01624@csls2816> Content-Type: text/plain; charset="US-ASCII" Dear Histonetters, One of my colleagues is having a problem with Dako's Permanent Liquid Red staining. She told me that she was getting a heavy background around the tissues. She is using DKDH's Untra-V-Block goat serum for blocking and the detection system is DKSH's ONE Alkaline Phosphatase Polymer. I use Dako's Fast Red Substrate system with Dako's labeled Polymer, AP (anti-mouse & anti-rabbit) without such problem. I was wondering what could cause such problems. I understand that Liquid Red is basically the same as the Fast Red. Is that true? Thank you in advance for your suggestion. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au ------------------------------ Message: 20 Date: Tue, 4 Dec 2007 09:09:12 +0200 From: "louise renton" Subject: Re: [Histonet] uranium - question for microscope slide manufacturers possibly To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 This is absolute free thinking here....... could this be contamination of some sort? Perhaps the lasering process "kicks" off some cells containing U if they are not tightly bound to the slide and they float around to settle and upset everyones measurements Try testing slides form a new, unopened box perferably from another distant lab or dierctly from the supplier or another supplier altogether. What is the background U content in Arizona? Think of the example of arsenic in exhumed bodies. The amount found in the tissue can be rendered meaningless if the levels in the surrounding soil around the bodies is significant. recalibrate the equipment with a known standard and retest Just my 2 cents worth On 12/4/07, Andrea Grantham wrote: > > I have a question about slides containing concentrations of uranium. > > One of my investigators is working on a project where they are > lasering tissue off of slides and checking it for the amount of > uranium in the tissue. The laser actually vaporizes the tissue and it > goes into a machine where it is tested for uranium content. They were > getting some strange readings and tested the slides alone without > tissue and found that the slides alone had a pretty high uranium content. > > So she is asking - Is there some coating to put on the slides to > block the uranium? > > My question is this - is there really uranium in slides and just how > much uranium is in a glass slide? Can this be dangerous? > > Are there some slides out there that do not contain uranium? > > I hope somebody has an answer for my weird question of the day. > > Thanks. > > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 21 Date: Tue, 4 Dec 2007 12:04:11 -0000 From: "Kemlo Rogerson" Subject: RE: [Histonet] Schiff's reagent To: "Taben Hale" , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2BA@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" Used to make this myself by bubbling SO2 through basic fuchsin, really good fun, but we can't do it now. The stock solution should be kept in the fridge and is OK when clear (if it starts to go pink discard). We used to satin up to 5 slides in a coplin jar which was stored in the fridge in between times. But then we just dripped a bit onto the section as it was felt it was better to waste small aliquots of the stain rather than risking the coplin jar contents becoming 'weak'. Schiff's was cheapish then as we made it ourselves but I guess it now costs a lot more made up? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 22 Date: Tue, 4 Dec 2007 04:41:52 -0800 (PST) From: Dawn Cowie Subject: Re: [Histonet] Florida licensure question To: Rene J Buesa , JMeade0710@aol.com, bethcoxx@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: <668430.6557.qm@web45012.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 2 companies that I have used. Florida Excel and Anderson Continuing Ed. They both sell CE materials and are recognised by state of Florida for obtaining your license. You can contact them both via internet. Dawn Rene J Buesa wrote: Same advise, e-mail the Florida Society for Histotechnologist. Ren? J. JMeade0710@aol.com wrote: I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get easy, one-click access to your favorites. Make Yahoo! your homepage. ------------------------------ Message: 23 Date: Tue, 4 Dec 2007 05:03:03 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] uranium - question for microscope slide manufacturers possibly To: Andrea Grantham , histonet@lists.utsouthwestern.edu Message-ID: <919617.96798.qm@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I don't think that any coating will "block" the uranium if it is present (a physics thing). Try to contact the manufacturer to find aout about the uranium contents of their raw (silicon) material. Ren? J. Andrea Grantham wrote: I have a question about slides containing concentrations of uranium. One of my investigators is working on a project where they are lasering tissue off of slides and checking it for the amount of uranium in the tissue. The laser actually vaporizes the tissue and it goes into a machine where it is tested for uranium content. They were getting some strange readings and tested the slides alone without tissue and found that the slides alone had a pretty high uranium content. So she is asking - Is there some coating to put on the slides to block the uranium? My question is this - is there really uranium in slides and just how much uranium is in a glass slide? Can this be dangerous? Are there some slides out there that do not contain uranium? I hope somebody has an answer for my weird question of the day. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. ------------------------------ Message: 24 Date: Tue, 4 Dec 2007 05:04:08 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Permanent Red stain To: Young Kwun , Histonet Message-ID: <327436.88165.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My advise is to contact DAKO. They are very cooperative and knowledgeable of their products. Ren? J. Young Kwun wrote: Dear Histonetters, One of my colleagues is having a problem with Dako's Permanent Liquid Red staining. She told me that she was getting a heavy background around the tissues. She is using DKDH's Untra-V-Block goat serum for blocking and the detection system is DKSH's ONE Alkaline Phosphatase Polymer. I use Dako's Fast Red Substrate system with Dako's labeled Polymer, AP (anti-mouse & anti-rabbit) without such problem. I was wondering what could cause such problems. I understand that Liquid Red is basically the same as the Fast Red. Is that true? Thank you in advance for your suggestion. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. ------------------------------ Message: 25 Date: Tue, 4 Dec 2007 07:44:29 -0600 From: "Dawson, Glen" Subject: RE: [Histonet] H Pylori To: "Mitchell, Jeannette M." , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Jeannette, We had a similar problem and solved it by switching to non-charged slides for our silver stains. It seems that the adhesive coating was taking up the stain. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mitchell, Jeannette M. Sent: Monday, December 03, 2007 3:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] H Pylori Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone?? Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 49, Issue 4 *************************************** From Jeannette.Mitchell <@t> vtmednet.org Tue Dec 4 10:18:27 2007 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Tue Dec 4 10:18:22 2007 Subject: [Histonet] fresh kidney bx In-Reply-To: <314881.98862.qm@web50610.mail.re2.yahoo.com> References: <314881.98862.qm@web50610.mail.re2.yahoo.com> Message-ID: We have gotten freezing artifact when the isopentane is not cold enough. We charge the isopentane with some dry ice and freezing artifact disappears. We also wash solution cat# 0103, Zeus Scientific and not PBS. Jeannette Mitchell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen dusek Sent: Tuesday, December 04, 2007 11:04 AM To: histonet Subject: [Histonet] fresh kidney bx Hi Everyone, I have been in Histology for a looong time and have met my match. I recently have new duties involving kidney bxs. We receive a formalin, EM, and Michels (Zuess) fixed specimens. The Formalin and EM bx are not an issue, the Michels is kicking by butt! I transfer the specimen from Michels to PBS, to rinse the fixative, for about 2-3 mins of agitation. After the PBS the specimen goes into the first OCT. This is swirled for another 2-3mins. I repeat this process again. My problem is I get specimens that have too much freezing artifact. The structures are "blown up" and not compact. I appreciate any suggestions to help the problem Thanks Eileen Dusek --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Dec 4 10:33:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 10:33:38 2007 Subject: [Histonet] fresh kidney bx In-Reply-To: <314881.98862.qm@web50610.mail.re2.yahoo.com> Message-ID: <164358.83295.qm@web61223.mail.yahoo.com> First of all, 2-3 minutes in PBS to wash out the Michell solution is too littler. I used to wash it on an agitation platform for 30 minutes. After that another wash and into distilled water and, believe it or not, I did not use OCT. In the cold chuck on the cold bar of the cryostat, I placed a small drop of distilled water, placed the biopsy (cut in half if it was a punch Bx, or on edge it is was a shave Bx), INTO de drop of water and very gently froze the drop of water, along with the Bx, with bursts of the Histofreeze. It worked wonders, and I did not have to remove the OCT afterwards. After many years doing it this way, I started using OCT and in the same way; OCT on the frozen chuck, Bx inside the OCT and freezing both with the coolant bursts. BUT, for you, at this moment, first try increasing the Michell washing time. Ren? J. eileen dusek wrote: Hi Everyone, I have been in Histology for a looong time and have met my match. I recently have new duties involving kidney bxs. We receive a formalin, EM, and Michels (Zuess) fixed specimens. The Formalin and EM bx are not an issue, the Michels is kicking by butt! I transfer the specimen from Michels to PBS, to rinse the fixative, for about 2-3 mins of agitation. After the PBS the specimen goes into the first OCT. This is swirled for another 2-3mins. I repeat this process again. My problem is I get specimens that have too much freezing artifact. The structures are "blown up" and not compact. I appreciate any suggestions to help the problem Thanks Eileen Dusek --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get easy, one-click access to your favorites. Make Yahoo! your homepage. From gagnone <@t> KGH.KARI.NET Tue Dec 4 11:54:07 2007 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Dec 4 11:54:21 2007 Subject: [Histonet] fresh kidney bx Message-ID: Eileen, we use commercially-prepared Michel's Transport Medium from Newcomer Supply. The renal biopsy is placed in this solution when taken and sent to our laboratory. Then we wash the specimen in Newcomer Supply's Michel's Buffered Wash Solution, for 3 x 10 minute washes (30 minutes total) and freeze the biopsy in OCT and cut for immunofluorescence. We've had very few preservation problems using these solutions. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From Bauer.Karen <@t> mayo.edu Tue Dec 4 12:19:54 2007 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Tue Dec 4 12:20:41 2007 Subject: [Histonet] Tissue Sections Message-ID: Hi to all... I know we've all talked about this before, and I've spent the last hour searching the archives for any information that I could use. Since there were no real answers to help me out, I'm turning to the pros for assistance. Lately we have been experiencing a lot of headaches with sections falling off, bad sections, and folding around the tissue edges. We have not changed anything that we have been doing and it's not all the slides. I asked the pathologist how many slides he's finding like that and he stated probably 15 to 20 slides a day. 15-20 out of 200 plus are coming out crappy, when all of the others are fine. Everything is processed, embedded, cut, stained and coverslipped the same, but some come out beautiful and some come out very bad. Whenever they ask for recuts on the bad slides, they say they are always beautiful. A lot of times, the recuts are cut by the same tech that cut it the first time. I've talked to the staff a lot about this occurrence and know all are knowledgeable of proper cutting techniques. They all turn in quality work. We have really slowed down our cutting in the morning in order to hand in that "perfect" slide. They macroscopically look great when we put them on the slide, but when the doctor gets them, crappy ones show up. We've thought about water trapped under the section and have let them drain vertically and air dry longer before we put them in the oven. We've let them cool after the oven to make sure the sections are adhered securely before putting the slides in xylene. We stain by hand, so we make sure we gently dip the slides and not dip aggressively. We coverslip by hand also. While coverslipping, there are many times that I see the edges of tissues move slightly when I put the coverslip on, so I know the tissue is not adhering. We have a slide etcher, so we use the ColorMark slides with the black backing. We use charged slides for all of our prostate cores, breast cores, and any small core biopsies that usually require special stains or IPs. We use plain ColorMark slides for all other routine Histology cases. We've tried using charged slides for all tissues, but were still getting bad slides here and there. As I said, all tissues are treated the same, but only 15-20 slides out of 200+ are coming out bad. Our techs rotate cutting, so I know it's not just cutting techniques. It's happening to all of us. It's gotten to the point that our doctors are focusing on the few bad slides and it's driving them crazy. I'm open for any suggestions. We are thinking about trying different slides or maybe putting an adhesive in the waterbath (yep, I know about adhesive and not using charged slides). Vendors who have different black backed slides than ColorMark for etching are welcome to reply. Thanks in advance, Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From mtarango <@t> nvcancer.org Tue Dec 4 13:22:09 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Dec 4 13:22:32 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <5C0BED61F529364E86309CADEA63FEF2F3AEA6@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B700D@NVCIEXCH02.NVCI.org> Sounds to me like she's working with some lazy Pathologists whom do not want to record the number of pieces during grossing. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Tuesday, December 04, 2007 7:17 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces The question would have been better in plain English, but even then, is missing essential detail without which the question is unanswerable. Submitted to whom, by whom, in what time interval, and what is being regarded as a biopsy in this particular context? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: 04 December 2007 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Janet.Bonner <@t> flhosp.org Tue Dec 4 13:42:56 2007 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Tue Dec 4 13:43:51 2007 Subject: [Histonet] Florida licensure question References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E11DE@fhosxchmb006.ADVENTISTCORP.NET> Anderson Continuing Education has that course. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of JMeade0710@aol.com Sent: Mon 12/3/2007 5:02 PM To: bethcoxx@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Florida licensure question I too am a traveler, and applying for the Florida license and in the same position that Beth is in. Any help would be appreciated. Jerry Meade In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time, bethcoxx@gmail.com writes: I work as a traveling tech, and do temporary assignments all over the country. I want to apply for my Florida license, but I need to fulfill that requirement for CE credits in "medical error prevention". Do any of you Florida people have suggestions on how/where I can get that without too much hassle?? Maybe something online? Thanks, Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************Check out AOL's list of 2007's hottest products. (http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From hej01 <@t> health.state.ny.us Tue Dec 4 14:03:56 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue Dec 4 14:04:09 2007 Subject: [Histonet] nasal passage frozen section Message-ID: Hi Histonetters, Is it possible to do frozen sections on nasal passages? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From FMonson <@t> wcupa.edu Tue Dec 4 14:45:39 2007 From: FMonson <@t> wcupa.edu (Monson, Frederick ) Date: Tue Dec 4 14:45:55 2007 Subject: [Histonet] uranium - question for microscope slide manufacturerspossibly In-Reply-To: <919617.96798.qm@web61217.mail.yahoo.com> References: <6.2.3.4.1.20071203153611.01ef6358@algranth.inbox.email.arizona.edu> <919617.96798.qm@web61217.mail.yahoo.com> Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A689@wcu-ex-emp2.PASSHE.LCL> Before the hysteria sets in, I have a suggestion. 1. Find out what levels of Uranium is being detected, and what the isotopes are. URL: http://www.atsdr.cdc.gov/tfacts150.pdf 2. Check for the radioactivity of the glass by grinding a gram of it to a powder and using a scintillation detector to determine the radioactivity. Read the attachment. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Light, Electron, X-Ray and Scanning Probe Imaging and Analysis Center (LEXSPIAC) Large Scientific Instrument Core West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu URL: http://lexspiac.wcupa.edu/casi ============================================ I have chosen to celebrate, during this Christmas season, the salvation of the Grinch as described by Dr. Seuss! ============================================ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2007 8:03 AM To: Andrea Grantham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] uranium - question for microscope slide manufacturerspossibly I don't think that any coating will "block" the uranium if it is present (a physics thing). Try to contact the manufacturer to find aout about the uranium contents of their raw (silicon) material. Ren? J. Andrea Grantham wrote: I have a question about slides containing concentrations of uranium. One of my investigators is working on a project where they are lasering tissue off of slides and checking it for the amount of uranium in the tissue. The laser actually vaporizes the tissue and it goes into a machine where it is tested for uranium content. They were getting some strange readings and tested the slides alone without tissue and found that the slides alone had a pretty high uranium content. So she is asking - Is there some coating to put on the slides to block the uranium? My question is this - is there really uranium in slides and just how much uranium is in a glass slide? Can this be dangerous? Are there some slides out there that do not contain uranium? I hope somebody has an answer for my weird question of the day. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FMonson <@t> wcupa.edu Tue Dec 4 14:49:20 2007 From: FMonson <@t> wcupa.edu (Monson, Frederick ) Date: Tue Dec 4 14:49:33 2007 Subject: [Histonet] Tissue Sections In-Reply-To: References: Message-ID: <641CEFFC7E5B6C42AB59539653FD08230517A68B@wcu-ex-emp2.PASSHE.LCL> Sounds like 10% dirty/oily slides, unless you clean them. Frederick C. Monson, PhD Technical Director Light, Electron, X-Ray and Scanning Probe Imaging and Analysis Center (LEXSPIAC) Large Scientific Instrument Core West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu URL: http://lexspiac.wcupa.edu/casi ============================================ I have chosen to celebrate, during this Christmas season, the salvation of the Grinch as described by Dr. Seuss! ============================================ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Tuesday, December 04, 2007 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Sections Hi to all... I know we've all talked about this before, and I've spent the last hour searching the archives for any information that I could use. Since there were no real answers to help me out, I'm turning to the pros for assistance. Lately we have been experiencing a lot of headaches with sections falling off, bad sections, and folding around the tissue edges. We have not changed anything that we have been doing and it's not all the slides. I asked the pathologist how many slides he's finding like that and he stated probably 15 to 20 slides a day. 15-20 out of 200 plus are coming out crappy, when all of the others are fine. Everything is processed, embedded, cut, stained and coverslipped the same, but some come out beautiful and some come out very bad. Whenever they ask for recuts on the bad slides, they say they are always beautiful. A lot of times, the recuts are cut by the same tech that cut it the first time. I've talked to the staff a lot about this occurrence and know all are knowledgeable of proper cutting techniques. They all turn in quality work. We have really slowed down our cutting in the morning in order to hand in that "perfect" slide. They macroscopically look great when we put them on the slide, but when the doctor gets them, crappy ones show up. We've thought about water trapped under the section and have let them drain vertically and air dry longer before we put them in the oven. We've let them cool after the oven to make sure the sections are adhered securely before putting the slides in xylene. We stain by hand, so we make sure we gently dip the slides and not dip aggressively. We coverslip by hand also. While coverslipping, there are many times that I see the edges of tissues move slightly when I put the coverslip on, so I know the tissue is not adhering. We have a slide etcher, so we use the ColorMark slides with the black backing. We use charged slides for all of our prostate cores, breast cores, and any small core biopsies that usually require special stains or IPs. We use plain ColorMark slides for all other routine Histology cases. We've tried using charged slides for all tissues, but were still getting bad slides here and there. As I said, all tissues are treated the same, but only 15-20 slides out of 200+ are coming out bad. Our techs rotate cutting, so I know it's not just cutting techniques. It's happening to all of us. It's gotten to the point that our doctors are focusing on the few bad slides and it's driving them crazy. I'm open for any suggestions. We are thinking about trying different slides or maybe putting an adhesive in the waterbath (yep, I know about adhesive and not using charged slides). Vendors who have different black backed slides than ColorMark for etching are welcome to reply. Thanks in advance, Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Dec 4 17:48:22 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Dec 4 17:48:35 2007 Subject: [Histonet] Re: Retic jargon Message-ID: Gitterfaser-Faerbung, reports Gudrun Lang in Linz, Austria - literally trellis fiber coloring. (Is "Faerbung" the correct German word for silver impregnation?) I too find the word "retic" easily confused between reticulocytes and reticulum stains. I'm surprised to learn that there is a significant amount of uranium in glass slides. Small amounts of uranyl ion give glass a fluorescent yellow color - that's "vaseline glass", also called "canary glass". Its manufacture was banned at the onset of WW II, and though it eventually became legal to make it again, the old pieces became eminently collectible - I have a few of them in fact - eBay usually provides a fine selection. I suppose that present day uranium salts are made from U235-depleted uranium (we have something like a billion pounds of depleted UF6 at Oak Ridge). That shouldn't change the radioactivity problems, since radium 226 (which accounts for most of the radioactivity of old stocks of uranium salts) is a decay product of U238. I agree with Fred Monson about nuke-u-lar. This barbarism is now beginning to appear in biomedical usage, for the cell nucleus. The first person I ever heard say nuke-you-lar was President Lyndon Johnson. Bob Richmond Samurai Pathologist Knoxville TN From RSRICHMOND <@t> aol.com Tue Dec 4 18:02:03 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Dec 4 18:02:15 2007 Subject: [Histonet] Re: Amyloid Control Message-ID: Lorraine Cornett in Kingsport TN (right up the road from Knoxville) asked for and got (yay Histonet) an amyloid control. She noted that paraffin sections do not last more than a couple of weeks, and need to be cut fairly fresh. Everybody has trouble getting amyloid controls - a famous academic surgical pathologist who first made his reputation with amyloid in George Glenner's laboratory, told me he didn't have one. What I don't understand is - amyloidosis is rather easily produced in some experimental animals by repeated injection of proteins such as casein. Why isn't experimental animal material offered commercially as an amyloid control? Medullary carcinomas of the thyroid usually contain some amyloid, and I've seen them successfully used as amyloid controls. There are many other problems with amyloid staining. Congo red staining requires a polarizing microscope, and many pathologists in small pathology services aren't allowed to have one of these. Does anybody have any experience with Anatech's Amyloid Red? Bob Richmond Samurai Pathologist Knoxville TN From Jason.Burrill <@t> crl.com Tue Dec 4 18:49:39 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Tue Dec 4 18:50:03 2007 Subject: [Histonet] Flammable Liquids Storage Question Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** From Annette.Fletcher <@t> providence.org Tue Dec 4 18:41:42 2007 From: Annette.Fletcher <@t> providence.org (Fletcher, Annette M) Date: Tue Dec 4 18:52:15 2007 Subject: [Histonet] 2 Histotech positions on Oregon Message-ID: <284596E350CB0743BB500B18475E7A0B479303@wn1223.or.providence.org> Providence Health and Services, a not-for-profit Healthcare System, is seeking 2 Histotechs in Oregon. We have a full-time, day-shift position in Southern, OR (Medford) which is rich with outdoor adventures and cultural amenities - golfing, fishing, rafting and snow skiing as well as world class theater and music venues. In Portland, OR we are looking for a full-time night shift Histotech. I'd love to answer any questions you have about these positions as well as Providence and the area. We are very competitive in terms of pay and have excellent benefits! Please contact me with any questions, Annette Fletcher Senior Recruiter Providence Regional Employment 1235 NE 47th Avenue, Ste 200 Portland, OR 97214 (503) 215-5840 annette.fletcher@providence.org DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From mickie25 <@t> netzero.net Tue Dec 4 19:02:17 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Dec 4 19:02:32 2007 Subject: [Histonet] Re: Amyloid Control In-Reply-To: References: Message-ID: Another source for Amyloid is tissues from an autopsy of a person who died of multiple myeloma. Often the kidneys are full of Amyloid. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, December 04, 2007 4:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Amyloid Control Lorraine Cornett in Kingsport TN (right up the road from Knoxville) asked for and got (yay Histonet) an amyloid control. She noted that paraffin sections do not last more than a couple of weeks, and need to be cut fairly fresh. Everybody has trouble getting amyloid controls - a famous academic surgical pathologist who first made his reputation with amyloid in George Glenner's laboratory, told me he didn't have one. What I don't understand is - amyloidosis is rather easily produced in some experimental animals by repeated injection of proteins such as casein. Why isn't experimental animal material offered commercially as an amyloid control? Medullary carcinomas of the thyroid usually contain some amyloid, and I've seen them successfully used as amyloid controls. There are many other problems with amyloid staining. Congo red staining requires a polarizing microscope, and many pathologists in small pathology services aren't allowed to have one of these. Does anybody have any experience with Anatech's Amyloid Red? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Tue Dec 4 19:13:23 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Dec 4 19:13:35 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <953583.62768.qm@web61223.mail.yahoo.com> References: <120420071501.23363.47556BDA000DE29F00005B4322134843739C030E0B0E020A9D0E05@comcast.net> <953583.62768.qm@web61223.mail.yahoo.com> Message-ID: Hi Histonetters! I think medically-legally, one has no defense if one is accused of loosing a diagnostic GI or liver biopsy piece, if the numbers of pieces received and processed (embedded, cut and stained) are not documented and I personally would want to be secure in the knowledge that ALL of my biopsy pieces were evaluated for cancer or some other disease. So there are two 'really good reasons' to document every scrap of biopsy tissue received. Now a days, diagnoses are made on tiny pieces of GI and prostate needle biopsy material we would have considered debris in the 'good old days' eh? If I sound like I'm flaming (sp?) I am not really, just serious about what a pathology team is responsible for and should be about. Thanks, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2007 7:45 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] recording Bx pieces Because that is the only objective way you have to determine if you processed them all! Ren? J. karenadams@comcast.net wrote: Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Dec 5 02:53:08 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Dec 5 02:53:23 2007 Subject: [Histonet] Flammable Liquids Storage Question Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2C4@wahtntex2.waht.swest.nhs.uk> "I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason" Makes no sense! If the alcohol is supplied in a container fit for purpose then why put such a potentially dangerous step into the procedure; pouring it into a safety can? If the containers are not fit for purpose then you shouldn't have received the alcohol in them in the first place. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From lpwenk <@t> sbcglobal.net Wed Dec 5 04:23:16 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Dec 5 04:23:29 2007 Subject: [Histonet] NSH Teleconference Message-ID: <000c01c83728$d981c880$0202a8c0@HPPav2> Blatant push for NSH teleconferences, so click delete now if you don't want to know anything more about them. The 2008 (and Dec. 2007) teleconferences are now listed on the NSH webpage http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&type ID=1183&itemID=17772 Or, go to http://www.nsh.org Click on Meetings on left Click on Teleconferences Held once a month on the fourth Wednesday of the month, from 1-2 pm Eastern time. Exceptions: - no teleconference in Sept. 2008 due to NSH S/C - Nov and Dec. 2008 will be the 3rd Wednesday, due to the holidays. Topics range from - special stains (bacteria, carbohydrates, kidney biopsies) - techniques (frozen sectioning, radiofrequency energy in surgery) - IHC (cytology specimens, prostate, her2/neu validation) - safety (substitutes, solvent recycling) - management (CPT coding) All those who attend, or who later listen to the CD and submit the test, receive 1 hour continuing education (CE), which can be used to satisfy CE requirements for your work, state licensure, or ASCP CMP. Cost is $125 for each, or $1100 for all 11 (that's only $100 each, for a savings of $275, if sign up before Jan. 18, 2008). Before the teleconference, you receive an email link to the PowerPoint presentation, handouts, sign in sheet and instruction sheet. After the teleconference, you receive a CD with all the same material PLUS the audio of the teleconference with a test. For anyone who could not attend the teleconference, they can take the CD, watch the PowerPoint, listen to the audio, read the handouts, take the test, and fax it to NSH and still receive 1 hour CE, up to 2 years after the original date of the teleconference. (And now the disclaimer - I'm the NSH Teleconference Coordinator, but no, I don't make any money by doing the responsibilities of this position or by getting more people to sign up for teleconference. My only goal in this is to help people obtain CE and become more knowledgable in our field.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From jcbook <@t> gmail.com Wed Dec 5 05:10:30 2007 From: jcbook <@t> gmail.com (J C) Date: Wed Dec 5 05:10:40 2007 Subject: [Histonet] CD 26 Message-ID: <52cb0f130712050310s18c89d19i489491195f05bf5e@mail.gmail.com> Dear Mark! I did not do it ever, but i know that many researches check CD26 by histochemistry and not by immunohistochemistry. (look e.g. Hepatology 2007 vol 47;p1971 in Supplements) or look other articles about DPPIV knockout. Julie Carmel . From jnocito <@t> satx.rr.com Wed Dec 5 05:59:41 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Dec 5 05:59:58 2007 Subject: [Histonet] Flammable Liquids Storage Question References: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> Message-ID: <003601c83736$51d98270$0302a8c0@yourxhtr8hvc4p> Jason, it's not required. Through all the inspections I've conducted and all the inspections I've been through, I never was cited for it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Burrill, Jason" To: Sent: Tuesday, December 04, 2007 6:49 PM Subject: [Histonet] Flammable Liquids Storage Question I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Dec 5 07:06:09 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Dec 5 07:06:25 2007 Subject: [Histonet] Flammable Liquids Storage Question In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F2C4@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F2C4@wahtntex2.waht.swest.nhs.uk> Message-ID: I agree! The only solvent we transferred to a red can was iso-pentane which came in a glass bottle. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Wednesday, December 05, 2007 12:53 AM To: Burrill, Jason; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flammable Liquids Storage Question "I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason" Makes no sense! If the alcohol is supplied in a container fit for purpose then why put such a potentially dangerous step into the procedure; pouring it into a safety can? If the containers are not fit for purpose then you shouldn't have received the alcohol in them in the first place. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 5 07:58:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 5 07:58:45 2007 Subject: [Histonet] Flammable Liquids Storage Question In-Reply-To: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> Message-ID: <724915.55355.qm@web61218.mail.yahoo.com> Plastic containers received from the manufacturer are deemed "safe" and can be used and stored as such. Safety containers are needed when you receive the flammables in large containers (like ethanol in drums) and you have to pump them out TO a small safety container that is going to be stores / used in the lab. You do not, and should not transfer a flammable from the original (usually 1 gal) plastic container to a metal safety container. For sure you are going to spill some amount and wil worsen the whole situation. Ren? J. "Burrill, Jason" wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From cmiller <@t> physlab.com Wed Dec 5 09:14:39 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Dec 5 09:15:19 2007 Subject: [Histonet] Flammable Liquids Storage Question In-Reply-To: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> References: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> Message-ID: <004801c83751$8e349af0$3402a8c0@plab.local> That makes no sense......seems like you would be OVER handling flammable liquids! Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burrill, Jason Sent: Tuesday, December 04, 2007 6:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable Liquids Storage Question I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From b-frederick <@t> northwestern.edu Wed Dec 5 09:49:03 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Dec 5 09:49:17 2007 Subject: [Histonet] Slide quality In-Reply-To: <648380.1758.qm@web57412.mail.re1.yahoo.com> Message-ID: <000101c83756$5c6cabc0$d00f7ca5@lurie.northwestern.edu> If you were doing your registry and had a SINGLE wrinkle you'd hear about it!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandy Smith Sent: Friday, November 30, 2007 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide quality When should the pathologist document wrinkles/folds? Only when it interferes with the diagnosis? Or any time there is a wrinkle or fold? Also how many histo techs out there get 100% wrinkle free sections? I have had discussions with my pathologists about these issues and would like others input. I believe any time there is a wrinkle or fold it should be documented. ____________________________________________________________________________ ________ Get easy, one-click access to your favorites. Make Yahoo! your homepage. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Wed Dec 5 10:04:44 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Wed Dec 5 10:07:13 2007 Subject: [Histonet] Slide quality In-Reply-To: <000101c83756$5c6cabc0$d00f7ca5@lurie.northwestern.edu> Message-ID: We are not permitted to have any wrinkles or folds for immunos. I think they will allow very small ones for all the H&Es and other special stains. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, December 05, 2007 9:49 AM To: 'Sandy Smith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide quality If you were doing your registry and had a SINGLE wrinkle you'd hear about it!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandy Smith Sent: Friday, November 30, 2007 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide quality When should the pathologist document wrinkles/folds? Only when it interferes with the diagnosis? Or any time there is a wrinkle or fold? Also how many histo techs out there get 100% wrinkle free sections? I have had discussions with my pathologists about these issues and would like others input. I believe any time there is a wrinkle or fold it should be documented. ________________________________________________________________________ ____ ________ Get easy, one-click access to your favorites. Make Yahoo! your homepage. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Dec 5 10:21:12 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Dec 5 10:21:34 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <120420071501.23363.47556BDA000DE29F00005B4322134843739C030E0B0E020A9D0E05@comcast.net> Message-ID: <000d01c8375a$dcf7c9b0$d00f7ca5@lurie.northwestern.edu> To cover your own butt! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: Tuesday, December 04, 2007 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Dec 5 10:29:21 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Dec 5 10:29:39 2007 Subject: [Histonet] Re: Amyloid Control In-Reply-To: Message-ID: <000f01c8375c$004f6020$d00f7ca5@lurie.northwestern.edu> When I was in Denver I was told that NSH has a control bank. Did anyone try them? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, December 04, 2007 6:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Amyloid Control Lorraine Cornett in Kingsport TN (right up the road from Knoxville) asked for and got (yay Histonet) an amyloid control. She noted that paraffin sections do not last more than a couple of weeks, and need to be cut fairly fresh. Everybody has trouble getting amyloid controls - a famous academic surgical pathologist who first made his reputation with amyloid in George Glenner's laboratory, told me he didn't have one. What I don't understand is - amyloidosis is rather easily produced in some experimental animals by repeated injection of proteins such as casein. Why isn't experimental animal material offered commercially as an amyloid control? Medullary carcinomas of the thyroid usually contain some amyloid, and I've seen them successfully used as amyloid controls. There are many other problems with amyloid staining. Congo red staining requires a polarizing microscope, and many pathologists in small pathology services aren't allowed to have one of these. Does anybody have any experience with Anatech's Amyloid Red? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed Dec 5 10:28:26 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Dec 5 10:30:09 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE011B700D@NVCIEXCH02.NVCI.org> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE576@EXCHANGEBE1.carle.com> I have been reading this with great interest. I think the issue of documenting number of biopsy pieces is more complex than it appears on the surface and the practice could actually become a liability as well as a help. Let's just say, for example, you document 6 prostate core biopsies and during processing one of those little blighters breaks in two. Now the block and slide have what looks like 7 prostate biopsies. If one of those cores show cancer and a lawyer looks at the records he could argue that the seventh core on the slide is actually from a different case and you have just caused his client to suffer great emotional stress. How many times has that little fleck of gi biopsy turned out to be mucus only to dissolve and vanish in the processor. Suddenly the 8 gi biopsy fragments become 7. My thoughts and feelings are that if I document a specific number at gross I have now obligated myself to that specific number even though things, beyond anyone's control, can occur during processing to change that number and come back to haunt me. Once something is documented and on the report you will be held accountable for that documentation and any variation will have to be explained and documented also. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, December 04, 2007 1:22 PM To: Marshall Terry Dr, Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces Sounds to me like she's working with some lazy Pathologists whom do not want to record the number of pieces during grossing. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Tuesday, December 04, 2007 7:17 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces The question would have been better in plain English, but even then, is missing essential detail without which the question is unanswerable. Submitted to whom, by whom, in what time interval, and what is being regarded as a biopsy in this particular context? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: 04 December 2007 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Dec 5 10:34:17 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Dec 5 10:34:36 2007 Subject: [Histonet] Re: Off topic-Radioactive jargon Message-ID: <120520071634.15319.4756D309000A1DED00003BD722007343649D09020704040A0105@comcast.net> All, just a bit of further explanation so people don't come up with misconceptions in trivial pursuit games about radioactive jargon or facts, there cannot be "billions" of pounds of UF6 (uranium hexaflouride which is a gas) in the world much less in Oak Ridge. I'm assuming Bob is using "billions" as a substitute word for large amounts. Even so, that is a stretch. UF6 is the gaseous uranium state used in a gaseous diffusion set-up to enrich for bomb grade uranium at Oak Ridge for WWII and (now in other places in the world????). Depleted gas is reduced to metal state simply to reduce the volume of the gas. There for sure is depleted U-238 around with the halting of the production of Pu-239 actually from feeder U-238. Or so says a nuclear physicist/engineer who has made his way around Oak Ridge, Hanford and Los Alamos and many other nuclear sites over the last 40 years. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Robert Richmond" > Gitterfaser-Faerbung, reports Gudrun Lang in Linz, Austria - literally > trellis fiber coloring. (Is "Faerbung" the correct German word for > silver impregnation?) > > I too find the word "retic" easily confused between reticulocytes and > reticulum stains. > > I'm surprised to learn that there is a significant amount of uranium > in glass slides. Small amounts of uranyl ion give glass a fluorescent > yellow color - that's "vaseline glass", also called "canary glass". > Its manufacture was banned at the onset of WW II, and though it > eventually became legal to make it again, the old pieces became > eminently collectible - I have a few of them in fact - eBay usually > provides a fine selection. > > I suppose that present day uranium salts are made from U235-depleted > uranium (we have something like a billion pounds of depleted UF6 at > Oak Ridge). That shouldn't change the radioactivity problems, since > radium 226 (which accounts for most of the radioactivity of old stocks > of uranium salts) is a decay product of U238. > > I agree with Fred Monson about nuke-u-lar. This barbarism is now > beginning to appear in biomedical usage, for the cell nucleus. The > first person I ever heard say nuke-you-lar was President Lyndon > Johnson. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Burrill <@t> crl.com Wed Dec 5 10:43:06 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Wed Dec 5 10:43:27 2007 Subject: [Histonet] Flammable Liquids Storage Question In-Reply-To: <003601c83736$51d98270$0302a8c0@yourxhtr8hvc4p> References: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> <003601c83736$51d98270$0302a8c0@yourxhtr8hvc4p> Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E86109A@shr-exch2.na01.crl.com> Joe and others, I completely agree. We just had a rather large vendor that makes safety cans and other flammable storage units come through my lab during an informal audit and say that we had to transfer all remaining Class I flammables from opened the original manufacturer's containers after the seal of the container was broken. I know that this is surprising to hear that a company would try to use this type of scare tactic to get you to buy their products. I asked them to site either the OSHA standard, which I am very familiar with, or the NFPA code that mandates this has to be done and they told me it was OSHA 29cfr 1910.106 and NFPA code 30 but when I looked at both of these neither one said that you had to transfer flammable liquids in 5 liters or less into safety cans. I also sent this person a link to the OSHA letter of interpretation that states that it is not necessary to transfer these liquids if it would compromise the purity of those reagents or result in corroding the safety can. I just wanted to see if others had been approached with this bogus claim and to just warn of vendors trying to use non-compliance as a way to sell their products. Thanks again for your responses, Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, December 05, 2007 7:00 AM To: Burrill, Jason; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquids Storage Question Jason, it's not required. Through all the inspections I've conducted and all the inspections I've been through, I never was cited for it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Burrill, Jason" To: Sent: Tuesday, December 04, 2007 6:49 PM Subject: [Histonet] Flammable Liquids Storage Question I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Wed Dec 5 10:44:11 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Dec 5 10:44:56 2007 Subject: [Histonet] recording Bx pieces In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE576@EXCHANGEBE1.carle.com> References: <5AEC610C1CE02945BD63A395BA763EDE011B700D@NVCIEXCH02.NVCI.org> <44780C571F28624DBB446DE55C4D733A1FE576@EXCHANGEBE1.carle.com> Message-ID: <000901c8375e$10345de0$3402a8c0@plab.local> Well said. We have come to the same conclusion. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, December 05, 2007 10:28 AM To: Tarango, Mark; Marshall Terry Dr,Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces I have been reading this with great interest. I think the issue of documenting number of biopsy pieces is more complex than it appears on the surface and the practice could actually become a liability as well as a help. Let's just say, for example, you document 6 prostate core biopsies and during processing one of those little blighters breaks in two. Now the block and slide have what looks like 7 prostate biopsies. If one of those cores show cancer and a lawyer looks at the records he could argue that the seventh core on the slide is actually from a different case and you have just caused his client to suffer great emotional stress. How many times has that little fleck of gi biopsy turned out to be mucus only to dissolve and vanish in the processor. Suddenly the 8 gi biopsy fragments become 7. My thoughts and feelings are that if I document a specific number at gross I have now obligated myself to that specific number even though things, beyond anyone's control, can occur during processing to change that number and come back to haunt me. Once something is documented and on the report you will be held accountable for that documentation and any variation will have to be explained and documented also. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, December 04, 2007 1:22 PM To: Marshall Terry Dr, Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces Sounds to me like she's working with some lazy Pathologists whom do not want to record the number of pieces during grossing. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Tuesday, December 04, 2007 7:17 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces The question would have been better in plain English, but even then, is missing essential detail without which the question is unanswerable. Submitted to whom, by whom, in what time interval, and what is being regarded as a biopsy in this particular context? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: 04 December 2007 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Dec 5 10:46:27 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Dec 5 10:46:43 2007 Subject: [Histonet] Re: Amyloid Control In-Reply-To: <000f01c8375c$004f6020$d00f7ca5@lurie.northwestern.edu> Message-ID: <898D946569A27444B65667A49C074052013D7623@mailbe06.mc.vanderbilt.edu> The NSH Quality Control committee does maintain a control bank. In this particular case, I actually extracted the original post from Ms. Cornett and forwarded it to our "librarian". We operate by members donating to the bank when they have excess control tissue. Likewise, if you need a control block, you can either ask the NSH office, or ask me, or post on histonet which I try to monitor for requests. There is a request form on the NSH webpage located on the QC committee page. We are here to help, if we can, when you need control blocks. Our librarian is amazing and responds promptly. If anyone has any extra control tissue to donate, please let us know. Have a great week. Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, December 05, 2007 10:29 AM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Amyloid Control When I was in Denver I was told that NSH has a control bank. Did anyone try them? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, December 04, 2007 6:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Amyloid Control Lorraine Cornett in Kingsport TN (right up the road from Knoxville) asked for and got (yay Histonet) an amyloid control. She noted that paraffin sections do not last more than a couple of weeks, and need to be cut fairly fresh. Everybody has trouble getting amyloid controls - a famous academic surgical pathologist who first made his reputation with amyloid in George Glenner's laboratory, told me he didn't have one. What I don't understand is - amyloidosis is rather easily produced in some experimental animals by repeated injection of proteins such as casein. Why isn't experimental animal material offered commercially as an amyloid control? Medullary carcinomas of the thyroid usually contain some amyloid, and I've seen them successfully used as amyloid controls. There are many other problems with amyloid staining. Congo red staining requires a polarizing microscope, and many pathologists in small pathology services aren't allowed to have one of these. Does anybody have any experience with Anatech's Amyloid Red? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 5 10:49:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 5 10:49:21 2007 Subject: [Histonet] Flammable Liquids Storage Question In-Reply-To: <1AD4E907E9B6F648AEF1B3A20A9B0E1E86109A@shr-exch2.na01.crl.com> Message-ID: <880077.16521.qm@web61211.mail.yahoo.com> Did you say this guy SELLS safety containers? Ren? J. "Burrill, Jason" wrote: Joe and others, I completely agree. We just had a rather large vendor that makes safety cans and other flammable storage units come through my lab during an informal audit and say that we had to transfer all remaining Class I flammables from opened the original manufacturer's containers after the seal of the container was broken. I know that this is surprising to hear that a company would try to use this type of scare tactic to get you to buy their products. I asked them to site either the OSHA standard, which I am very familiar with, or the NFPA code that mandates this has to be done and they told me it was OSHA 29cfr 1910.106 and NFPA code 30 but when I looked at both of these neither one said that you had to transfer flammable liquids in 5 liters or less into safety cans. I also sent this person a link to the OSHA letter of interpretation that states that it is not necessary to transfer these liquids if it would compromise the purity of those reagents or result in corroding the safety can. I just wanted to see if others had been approached with this bogus claim and to just warn of vendors trying to use non-compliance as a way to sell their products. Thanks again for your responses, Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, December 05, 2007 7:00 AM To: Burrill, Jason; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquids Storage Question Jason, it's not required. Through all the inspections I've conducted and all the inspections I've been through, I never was cited for it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Burrill, Jason" To: Sent: Tuesday, December 04, 2007 6:49 PM Subject: [Histonet] Flammable Liquids Storage Question I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Terry.Marshall <@t> rothgen.nhs.uk Wed Dec 5 10:54:26 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Dec 5 10:54:43 2007 Subject: [Histonet] recording Bx pieces Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AEAE@TRFT-EX01.xRothGen.nhs.uk> Charles, I think that although your points are well taken (and partly addressed by me in a previous post), you are over-dramatising, It is not written in stone that what goes in has to come out. Since it is well known that bits disappear because they are mucus, fibrin, faeces or whatever, or that parts break off, then what is really the problem? In the scenario you outline, there is no reason for a lawyer to get involved, and if he did, no reason to pursue the line you talk about, and even less reason to jump to the conclusion that one (the extra) piece is from elsewhere. If you worry about such things, you should get yourself a calliper with a Vernier and measure the fragments carefully; then a little arithmetic will see things right:-) Certainly, these worries do not override the necessity for all bits to be looked at, and you need to know how many there were to miss one. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: 05 December 2007 16:28 To: Tarango, Mark; Marshall Terry Dr, Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces I have been reading this with great interest. I think the issue of documenting number of biopsy pieces is more complex than it appears on the surface and the practice could actually become a liability as well as a help. Let's just say, for example, you document 6 prostate core biopsies and during processing one of those little blighters breaks in two. Now the block and slide have what looks like 7 prostate biopsies. If one of those cores show cancer and a lawyer looks at the records he could argue that the seventh core on the slide is actually from a different case and you have just caused his client to suffer great emotional stress. How many times has that little fleck of gi biopsy turned out to be mucus only to dissolve and vanish in the processor. Suddenly the 8 gi biopsy fragments become 7. My thoughts and feelings are that if I document a specific number at gross I have now obligated myself to that specific number even though things, beyond anyone's control, can occur during processing to change that number and come back to haunt me. Once something is documented and on the report you will be held accountable for that documentation and any variation will have to be explained and documented also. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, December 04, 2007 1:22 PM To: Marshall Terry Dr, Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces Sounds to me like she's working with some lazy Pathologists whom do not want to record the number of pieces during grossing. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Tuesday, December 04, 2007 7:17 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces The question would have been better in plain English, but even then, is missing essential detail without which the question is unanswerable. Submitted to whom, by whom, in what time interval, and what is being regarded as a biopsy in this particular context? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: 04 December 2007 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Dec 5 11:00:20 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Dec 5 11:00:32 2007 Subject: [Histonet] Re: recording Bx pieces Message-ID: Karen Adams at Pathology Laboratories West (in Knoxville TN, with walking distance of where I live - had my aortic valve replacement at Park West 5 years ago) asks: >>Could someone please give me a really good reason to relay to a pathologist as to why it is beneficial to record the number of [biopsy specimens] submitted?<< Let me turn this question around - why can't I get the embedder to embed using my laboriously handwritten record of how many specimens I put in the cassette, and use magnification while embedding them? Because that's not the way we did it at dear old Siwash. As biopsy specimens get smaller and smaller, it's too easy to lose them in process. It doesn't make sense to subject a patient to a procedure costing many thousands of dollars and much discomfort, and then not bother to find and account for all the specimens. The submitting clinical service needs to specify the number of specimens in the bottle. The pathologist (or pathologist's assistant) needs to count them, and record the count. And the embedder needs to have that count record in front of them when they embed. I think anything less is below standard of care. Vox clamantis in deserto. Bob Richmond Samurai Pathologist Knoxville TN From RSRICHMOND <@t> aol.com Wed Dec 5 11:06:57 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Dec 5 11:07:09 2007 Subject: [Histonet] Re: nasal passage frozen section Message-ID: Hazel Johnson asks if it's possible to do a frozen section on a nasal passage specimen. Physically, it doesn't present a problem unless there's bone or a lot of cartilage in it. Diagnostically, it could be quite useful. If getting a frozen section is going to change what the surgeon does to the patient at that same procedure, then the frozen section should be done. But this is a question for a pathologist, a question that shouldn't be dumped onto a histotechnologist! Bob Richmond Samurai Pathologist Knoxville TN From Jonathan.Arzt <@t> ARS.USDA.GOV Wed Dec 5 11:20:12 2007 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Wed Dec 5 11:22:06 2007 Subject: [Histonet] HT Job Opening, USDA, Plum Island, NY/CT Message-ID: <6B4AF69268EFAE4C80A786BA99F7A66701530077@MD-MAIL-01.ARSNET.ARS.USDA.GOV> BIODEFENSE HT/HTL POSITION AVAILABLE (PLUM ISLAND, NY/CT) A unique, full-time, permanent histotech. position is immediately available and advertised by the pathology division of the USDA, Animal Research Service on Plum Island, NY. The position will be offered at the grades of GS-7/9/11 conferring salary of $39K-$76K commensurate with experience and training. Benefits and promotion potential are excellent. Licensed HT and HTL preferred, but unlicensed candidates with appropriate experience will be considered and are encouraged to apply. The primary mission of the research group is investigation of veterinary diseases of potential threat to US agriculture interests. Currently the greatest emphases are directed towards characterizing the pathogenesis of foot-and-mouth disease and classical swine fever. The Plum Island Animal Disease Center is located off the East end of Long Island, NY. Transport to and from the island is provided free to employees aboard government-operated ferries servicing Orient, NY and Old Saybrook, CT. Rural and suburban communities are abundant near both the NY and CT sides of the ferries with access to scenic beaches and vineyards. New York City and Boston are both within 2-4hrs drive. Activities will be varied but will include conventional histotech work with paraffin-embedded and frozen tissues, immunohistochemistry, in-situ hybridization, confocal microscopy, and assistance with animal necropsies and sample collection. The position is currently advertised at: www.usajobs.com as announcement #: ARS-X8E-0001R . Open period for application is through December 31, 2007. For more information, email to: Jonathan.Arzt@ARS.USDA.GOV If applying, please email application materials directly to this address in addition to filing the application as described at USAJOBS. Note: this is an informational notice only and does not constitute an official advertisement of any position. From RSRICHMOND <@t> aol.com Wed Dec 5 11:23:23 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Dec 5 11:23:34 2007 Subject: [Histonet] Re: Slide quality Message-ID: You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Bob Richmond Samurai Pathologist Knoxville TN From Terry.Marshall <@t> rothgen.nhs.uk Wed Dec 5 11:39:10 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Dec 5 11:39:26 2007 Subject: [Histonet] Re: Slide quality Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AEAF@TRFT-EX01.xRothGen.nhs.uk> Endorse those views wholeheartedly. Wrinkles and scores don't spoil the slide for diagnosis - there's a whole heap of other ways to do that. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 05 December 2007 17:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wstover <@t> vet.uga.edu Wed Dec 5 12:27:50 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Wed Dec 5 12:28:03 2007 Subject: [Histonet] Immunohistochemistry. Message-ID: The University of Georgia will be holding an immunohistochemistry workshop on Jan. 12, 2008. It will provide 7.5 CEU. If possible (but not necessary) please register by Dec. 14, 2007. Please use link below for all info and registration. Thanks and hope to see you there!! Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From CIngles <@t> uwhealth.org Wed Dec 5 12:25:47 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Dec 5 12:30:51 2007 Subject: [Histonet] nasal passage frozen section References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200AB@uwhis-xchng3.uwhis.hosp.wisc.edu> Helen: Where on the nose are you talking? Is it more towards the tip, or the deep sinius areas inside the skull? The former, yes. We routinely do frozen sections of the Turbinate "bone" area, as it is only cartilage. The latter probably needs to be decaled before sectioning, depending on size. I am assuming you are talking about human tissue here. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Helen E Johnson Sent: Tue 12/4/2007 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nasal passage frozen section Hi Histonetters, Is it possible to do frozen sections on nasal passages? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Dec 5 12:45:57 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Dec 5 12:46:09 2007 Subject: [Histonet] buffered verses unbuffered EDTA for decalcification for tunel Message-ID: I need some help in determining if I need to use buffered or unbuffered EDTA to decal samples for tunel. I have searched the histonet and there are some protocols for buffered EDTA, but I have also seen protocols that the EDTA is unbuffered. Any help is appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From Rcartun <@t> harthosp.org Wed Dec 5 14:12:44 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 5 14:13:03 2007 Subject: [Histonet] CDX2 and HCA (hepatocellular carcinoma) In-Reply-To: <0A57D6AEAE4CBA4A984D27257160A72D01069D0B@win03exchange01.wtbyhosp.org> References: <0A57D6AEAE4CBA4A984D27257160A72D01069D0B@win03exchange01.wtbyhosp.org> Message-ID: <4756BFEC02000077000098C7@gwmail4.harthosp.org> We use a CDX2 mAb from BioGenex. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Piche-Grocki, Jessica" 11/30/07 12:33 PM >>> Hello, I am having a hard time finding information for our pathologists on CDX2 and HCA antibodies. If anyone has any information they can share that would be great. I am especially having a hard time with the HCA(hepatocellular carcinoma antigen). Thanks and have a great weekend!! Jessica Piche-Grocki, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Wed Dec 5 14:24:51 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 5 14:25:01 2007 Subject: [Histonet] buffered verses unbuffered EDTA for decalcification for tunel In-Reply-To: Message-ID: <146887.84996.qm@web61217.mail.yahoo.com> EDTA in itself is a buffered solution once you equilibrate its pH during preparation. Ren? J. Liz Chlipala wrote: I need some help in determining if I need to use buffered or unbuffered EDTA to decal samples for tunel. I have searched the histonet and there are some protocols for buffered EDTA, but I have also seen protocols that the EDTA is unbuffered. Any help is appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From gmartin <@t> marshallmedical.org Wed Dec 5 15:14:06 2007 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Dec 5 15:14:17 2007 Subject: [Histonet] Variation in staining Message-ID: <6ED9D4252F278841A0593D3D788AF24C0152DBEC@mailsvr.MARSHMED.local> In the last two days we have noticed a problem with our sections having areas that do not take up the Hematoxylin. This seems to be geographic pattern that varies at different levels. For example one level on the same slide may show this effect, while the next level will be fine. The Eosin is not effected. We have had no changes in our processing. I did search the archives but was unable to get results. Thank you From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Dec 6 03:58:57 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Dec 6 03:59:13 2007 Subject: [Histonet] Re: Slide quality Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2DD@wahtntex2.waht.swest.nhs.uk> You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Robert I'm surprised that you don't look at the slides with the Tech; how does he/ she know if they are doing a good job. In my experience when my Pathologist was kind enough to teach me some smatterings of Dermatopathology it was stunningly powerful in showing me the error of my ways. I don't understand why Techs/ BMSs don't look at slides before they go to the Pathologist and even take a crack at the diagnosis. On one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't spotted by the Pathologist but then I was a Cytologist; wouldn't be any good at anything non- cervical; but I could learn!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Dec 6 04:10:11 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Dec 6 04:10:26 2007 Subject: [Histonet] Re: Slide quality Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2DE@wahtntex2.waht.swest.nhs.uk> I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Bob Why would they feel the need to be abusive? Why would the Technologist find it unendurable? Tell you one thing Bob, it wouldn't happen with me! I'm not impressed with abuse nor am I likely to find it unendurable. If both tribes could learn to speak properly to each other then maybe both could learn something from each other. Maybe the quality of the slides would increase too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Dec 6 04:14:08 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Dec 6 04:14:23 2007 Subject: [Histonet] Variation in staining Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F2DF@wahtntex2.waht.swest.nhs.uk> In the last two days we have noticed a problem with our sections having areas that do not take up the Hematoxylin. This seems to be geographic pattern that varies at different levels. For example one level on the same slide may show this effect, while the next level will be fine. The Eosin is not effected. We have had no changes in our processing. Odd problem. I do remember having similar problems of patchy staining when using a rather 'weak' batch of haematoxylin (I grew my own); the non patchy nature of the eosin suggests the usual culprit of poor dewaxing of the slides is not responsible. Have you changed the batch of Haematoxylin recently? Is it past its sell by date? Try a new batch or another make. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jnocito <@t> satx.rr.com Thu Dec 6 06:06:37 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 6 06:06:55 2007 Subject: [Histonet] Re: Slide quality References: <5C0BED61F529364E86309CADEA63FEF2F3AEAF@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <009301c83800$746ac950$0302a8c0@yourxhtr8hvc4p> have you seen some of the printed photomics lately in journals and even in some texts? I was flipping through a placenta pathology book and I couldn't count the number of wrinkles and folds in the umbilical cords and membrane rolls. And this was in PRINT!!! I recently left a place where the standards were set so high ( or they had me to complain to) that even though 97% of ALL material being produced was of excellent quality, everyone focused on the other 3%. I was told that I needed to strive for 100%. I told the medical director that if I had 97% through out college, I'd be a pathologist. With the shortage of techs, the time constraints that most of us are under and the pressure to do more with less, something has to give. Sometimes do do happens and then you step in it. There are a lot of unreasonable people out there. Do the best you can every time, know in your heart that you did your best for that day and get some sleep. I learned the hard way that you can't please all the people all the time. There is a country song from Aaron Tippin that has a line "what ever you do during the day, you'll have to sleep with at night" That's my philosophy now and I'm sticking to it. OK, I'm off my soap box now. Is it Friday yet? JTT ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Robert Richmond" ; Sent: Wednesday, December 05, 2007 11:39 AM Subject: RE: [Histonet] Re: Slide quality Endorse those views wholeheartedly. Wrinkles and scores don't spoil the slide for diagnosis - there's a whole heap of other ways to do that. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 05 December 2007 17:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Dec 6 06:08:45 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 6 06:09:01 2007 Subject: [Histonet] Flammable Liquids Storage Question References: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> <003601c83736$51d98270$0302a8c0@yourxhtr8hvc4p> <1AD4E907E9B6F648AEF1B3A20A9B0E1E86109A@shr-exch2.na01.crl.com> Message-ID: <00a501c83800$c0818bd0$0302a8c0@yourxhtr8hvc4p> let the flaming begin (get it? I'm such the knee slapper). Who is this company? Let me stir the pot. JTT ----- Original Message ----- From: "Burrill, Jason" To: "Joe Nocito" ; Sent: Wednesday, December 05, 2007 10:43 AM Subject: RE: [Histonet] Flammable Liquids Storage Question Joe and others, I completely agree. We just had a rather large vendor that makes safety cans and other flammable storage units come through my lab during an informal audit and say that we had to transfer all remaining Class I flammables from opened the original manufacturer's containers after the seal of the container was broken. I know that this is surprising to hear that a company would try to use this type of scare tactic to get you to buy their products. I asked them to site either the OSHA standard, which I am very familiar with, or the NFPA code that mandates this has to be done and they told me it was OSHA 29cfr 1910.106 and NFPA code 30 but when I looked at both of these neither one said that you had to transfer flammable liquids in 5 liters or less into safety cans. I also sent this person a link to the OSHA letter of interpretation that states that it is not necessary to transfer these liquids if it would compromise the purity of those reagents or result in corroding the safety can. I just wanted to see if others had been approached with this bogus claim and to just warn of vendors trying to use non-compliance as a way to sell their products. Thanks again for your responses, Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, December 05, 2007 7:00 AM To: Burrill, Jason; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquids Storage Question Jason, it's not required. Through all the inspections I've conducted and all the inspections I've been through, I never was cited for it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Burrill, Jason" To: Sent: Tuesday, December 04, 2007 6:49 PM Subject: [Histonet] Flammable Liquids Storage Question I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Dec 6 06:14:02 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 6 06:14:19 2007 Subject: [Histonet] recording Bx pieces References: <5C0BED61F529364E86309CADEA63FEF2F3AEAE@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <00d401c83801$7deff620$0302a8c0@yourxhtr8hvc4p> what I do at grossing is write in pencil, on the side of the cassette, the number of pieces in the cassette, especially for G.I bxs. What I was putting in, wasn't coming out. A thorough investigation was done finding out that the embedder wasn't looking at the lids and sure enough, a piece was stuck there. I did correct the problem. JTT ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Charles.Embrey" ; "Tarango, Mark" ; ; Sent: Wednesday, December 05, 2007 10:54 AM Subject: RE: [Histonet] recording Bx pieces Charles, I think that although your points are well taken (and partly addressed by me in a previous post), you are over-dramatising, It is not written in stone that what goes in has to come out. Since it is well known that bits disappear because they are mucus, fibrin, faeces or whatever, or that parts break off, then what is really the problem? In the scenario you outline, there is no reason for a lawyer to get involved, and if he did, no reason to pursue the line you talk about, and even less reason to jump to the conclusion that one (the extra) piece is from elsewhere. If you worry about such things, you should get yourself a calliper with a Vernier and measure the fragments carefully; then a little arithmetic will see things right:-) Certainly, these worries do not override the necessity for all bits to be looked at, and you need to know how many there were to miss one. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: 05 December 2007 16:28 To: Tarango, Mark; Marshall Terry Dr, Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces I have been reading this with great interest. I think the issue of documenting number of biopsy pieces is more complex than it appears on the surface and the practice could actually become a liability as well as a help. Let's just say, for example, you document 6 prostate core biopsies and during processing one of those little blighters breaks in two. Now the block and slide have what looks like 7 prostate biopsies. If one of those cores show cancer and a lawyer looks at the records he could argue that the seventh core on the slide is actually from a different case and you have just caused his client to suffer great emotional stress. How many times has that little fleck of gi biopsy turned out to be mucus only to dissolve and vanish in the processor. Suddenly the 8 gi biopsy fragments become 7. My thoughts and feelings are that if I document a specific number at gross I have now obligated myself to that specific number even though things, beyond anyone's control, can occur during processing to change that number and come back to haunt me. Once something is documented and on the report you will be held accountable for that documentation and any variation will have to be explained and documented also. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Tuesday, December 04, 2007 1:22 PM To: Marshall Terry Dr, Consultant Histopathologist; karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces Sounds to me like she's working with some lazy Pathologists whom do not want to record the number of pieces during grossing. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Tuesday, December 04, 2007 7:17 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] recording Bx pieces The question would have been better in plain English, but even then, is missing essential detail without which the question is unanswerable. Submitted to whom, by whom, in what time interval, and what is being regarded as a biopsy in this particular context? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: 04 December 2007 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recording Bx pieces Could someone please give me a "really" good reason to relay to a pathologists as to why it is beneficial to record the # of bx's submitted?? ASAP! :) -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Thu Dec 6 06:41:49 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Dec 6 06:42:06 2007 Subject: [Histonet] Re: Slide quality In-Reply-To: <009301c83800$746ac950$0302a8c0@yourxhtr8hvc4p> References: <5C0BED61F529364E86309CADEA63FEF2F3AEAF@TRFT-EX01.xRothGen.nhs.uk> <009301c83800$746ac950$0302a8c0@yourxhtr8hvc4p> Message-ID: Three Cheers Joe! My realization came 8 years ago. I 'retired' and look what that has got me into! :) You don't know what is around the corner until you look. For the record, I would like to know who the metal can vendor is too! Flame On! Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 06, 2007 4:07 AM To: Marshall Terry Dr,Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Slide quality have you seen some of the printed photomics lately in journals and even in some texts? I was flipping through a placenta pathology book and I couldn't count the number of wrinkles and folds in the umbilical cords and membrane rolls. And this was in PRINT!!! I recently left a place where the standards were set so high ( or they had me to complain to) that even though 97% of ALL material being produced was of excellent quality, everyone focused on the other 3%. I was told that I needed to strive for 100%. I told the medical director that if I had 97% through out college, I'd be a pathologist. With the shortage of techs, the time constraints that most of us are under and the pressure to do more with less, something has to give. Sometimes do do happens and then you step in it. There are a lot of unreasonable people out there. Do the best you can every time, know in your heart that you did your best for that day and get some sleep. I learned the hard way that you can't please all the people all the time. There is a country song from Aaron Tippin that has a line "what ever you do during the day, you'll have to sleep with at night" That's my philosophy now and I'm sticking to it. OK, I'm off my soap box now. Is it Friday yet? JTT ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Robert Richmond" ; Sent: Wednesday, December 05, 2007 11:39 AM Subject: RE: [Histonet] Re: Slide quality Endorse those views wholeheartedly. Wrinkles and scores don't spoil the slide for diagnosis - there's a whole heap of other ways to do that. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 05 December 2007 17:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Burrill <@t> crl.com Thu Dec 6 07:18:48 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Thu Dec 6 07:19:04 2007 Subject: [Histonet] Flammable Liquids Storage Question In-Reply-To: <00a501c83800$c0818bd0$0302a8c0@yourxhtr8hvc4p> References: <1AD4E907E9B6F648AEF1B3A20A9B0E1E74BD3A@shr-exch2.na01.crl.com> <003601c83736$51d98270$0302a8c0@yourxhtr8hvc4p> <1AD4E907E9B6F648AEF1B3A20A9B0E1E86109A@shr-exch2.na01.crl.com> <00a501c83800$c0818bd0$0302a8c0@yourxhtr8hvc4p> Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E861250@shr-exch2.na01.crl.com> Joe, It was local representative of this company that made this claim so I don't want to throw an umbrella over the entire company just in case. I will tell you that they sell the lion's share of all flame cabinets used in Histology Labs. Sorry I can't fan your flaming. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Thursday, December 06, 2007 7:09 AM To: Burrill, Jason; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquids Storage Question let the flaming begin (get it? I'm such the knee slapper). Who is this company? Let me stir the pot. JTT ----- Original Message ----- From: "Burrill, Jason" To: "Joe Nocito" ; Sent: Wednesday, December 05, 2007 10:43 AM Subject: RE: [Histonet] Flammable Liquids Storage Question Joe and others, I completely agree. We just had a rather large vendor that makes safety cans and other flammable storage units come through my lab during an informal audit and say that we had to transfer all remaining Class I flammables from opened the original manufacturer's containers after the seal of the container was broken. I know that this is surprising to hear that a company would try to use this type of scare tactic to get you to buy their products. I asked them to site either the OSHA standard, which I am very familiar with, or the NFPA code that mandates this has to be done and they told me it was OSHA 29cfr 1910.106 and NFPA code 30 but when I looked at both of these neither one said that you had to transfer flammable liquids in 5 liters or less into safety cans. I also sent this person a link to the OSHA letter of interpretation that states that it is not necessary to transfer these liquids if it would compromise the purity of those reagents or result in corroding the safety can. I just wanted to see if others had been approached with this bogus claim and to just warn of vendors trying to use non-compliance as a way to sell their products. Thanks again for your responses, Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, December 05, 2007 7:00 AM To: Burrill, Jason; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquids Storage Question Jason, it's not required. Through all the inspections I've conducted and all the inspections I've been through, I never was cited for it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Burrill, Jason" To: Sent: Tuesday, December 04, 2007 6:49 PM Subject: [Histonet] Flammable Liquids Storage Question I have a question regarding the transfer of flammable liquids (reagent alcohol, methanol or xylene) to safety cans. Has anyone been required to transfer the remaining flammable liquids from the one gallon plastic or glass bottle it was supplied in from the vendor to a safety can after dispensing any amount from it? Thanks in advance for all your responses. Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcokie <@t> yahoo.com Thu Dec 6 08:19:42 2007 From: jcokie <@t> yahoo.com (Jeanenne Ely) Date: Thu Dec 6 08:19:57 2007 Subject: [Histonet] Job opening in Houston Message-ID: <470357.54325.qm@web81410.mail.mud.yahoo.com> Hello Histonetters- We have an opening for a Research Histology Technician at MD Anderson Cancer Center in Houston, TX, in the Veterinary Medicine and Surgery Department. This is a full time position, and a detailed job description can be accessed at www.mdanderson.org. If interested, you may apply online. Thanks, Jeanenne Ely, BS, HT (ASCP), QIHC Chief Histology Lab Vet Med & Surgery MD Anderson Cancer Center 1515 Holcombe Blvd Houston, TX 77030 --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From POWELL_SA <@t> Mercer.edu Thu Dec 6 09:36:38 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Dec 6 09:40:20 2007 Subject: [Histonet] IHC workshop basics Message-ID: <01MOKF63KOKG001IUP@Macon2.Mercer.edu> There will be an IHC basic workshop at the University of Georgia in Athens, Georgia Saturday January 12, 2008. This will be a great workshop for those just beginning in immunohistochemistry, students of histotechnology, research techs, and anyone else interested in the basics. Sessions to be held at the College of Veterinary Medicine in Room H-237 unless otherwise stated on program. Please go to http://www.georgiacenter.uga.edu/conferences/2008/Jan/12/immuno.phtml for information on the program, speakers, registration form, fees and lodging. From mlm11 <@t> cornell.edu Thu Dec 6 10:18:13 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Thu Dec 6 10:17:14 2007 Subject: [Histonet] want to buy processor Message-ID: <6.2.1.2.2.20071206103944.0405c858@postoffice9.mail.cornell.edu> Hello, It's time for me buy a tissue processor. Due to lack of room, I need a benchtop dunk and dip. I have a fume hood. Would all vendors of new and/or refurbished equipment send me quotes please? Thank you very much, Mary Lou Norman From cmiller <@t> physlab.com Thu Dec 6 11:21:46 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Dec 6 11:21:43 2007 Subject: [Histonet] reticulin/snooks Message-ID: <000601c8382c$7a494dc0$3402a8c0@plab.local> Who uses Snook's method for their retic stain?? If you do where do you get your uranium nitrate?? I ask Rowley and they don't supply it anymore because of it's hazards?? Am I missing something??? Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From nbroadbent <@t> ucsd.edu Thu Dec 6 12:03:15 2007 From: nbroadbent <@t> ucsd.edu (Nicola J. Broadbent Ph.D) Date: Thu Dec 6 12:01:12 2007 Subject: [Histonet] Interrupted thionin staining Message-ID: <002d01c83832$46d7b0c0$8a4cef84@Nicola> Hi, I would like to know whether it is ok to place rat brain tissue (50um slices on glass gelatin coated slides) directly from xylene to 100% alcholol to start the thionin staining process. Normally, we defat in a mix of chloroform and alcohol (approx 1 hr; which we did do with these brain sections) but due to being evacuated from our building we had to stop the staining process and in to store the tissue (as we were unsure how long we would be away from the lab) we placed the tissue in the zylene. The sections ended up being in zylene for approx 24 hrs. Would it be ok just to start the staining process from this point i.e. go straight to the 100%, 95% 70% alcohol steps from the zylene? Thanks so much for your help, Nicola Nicola J. Broadbent Ph.D, Asst. Project Scientist Department of Psychiatry 0603, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA, 92093-0603 Email: nbroadbent@ucsd.edu Phone: (858) 642 3628 or (858) 552 8585 x 7853 Fax: (858) 552 7457 From mhasu <@t> ottawaheart.ca Thu Dec 6 12:33:35 2007 From: mhasu <@t> ottawaheart.ca (Mirela Hasu) Date: Thu Dec 6 12:34:12 2007 Subject: [Histonet] immunohitochem for IFN gamma Message-ID: Hello, Does anybody have performed the immunohistochemistry for Interferon gamma on mice heart tissue and on aortic lesions. Would be very good if I can get a protocol that worked well. Thank you, Mirela From oshel1pe <@t> cmich.edu Thu Dec 6 12:34:00 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Dec 6 12:34:26 2007 Subject: [Histonet] reticulin/snooks In-Reply-To: <000601c8382c$7a494dc0$3402a8c0@plab.local> References: <000601c8382c$7a494dc0$3402a8c0@plab.local> Message-ID: Cheri, The electron microscopy supply companies are the usual places for uranyl salts. These are Biological Stain Commission certified, but they are the pure (or nearly) powders. Electron Microscopy Supplies http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx Structure Probe, Inc. aka SPI http://www.2spi.com/catalog/chem/stain.shtml Also, regular chemical companies like Sigma-Aldrich have uranyl nitrate. http://www.sigmaaldrich.com/catalog/search/SearchResultsPage/PricingAvailability/FLUKA;94270 Phil >Who uses Snook's method for their retic stain?? If you do where do you get >your uranium nitrate?? I ask Rowley and they don't supply it anymore because >of it's hazards?? Am I missing something??? > >Cheri Miller HT ASCP > >Histology Supervisor >Physicians Laboratory Services, Inc. > >Omaha, NE 68117 > >402 738 5052 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From gmartin <@t> marshallmedical.org Thu Dec 6 13:27:02 2007 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Dec 6 13:27:15 2007 Subject: [Histonet] Patchy staining Message-ID: <6ED9D4252F278841A0593D3D788AF24C0152E46B@mailsvr.MARSHMED.local> Thank you to all who responded to my request for help concerning the voids/patchy staining. It seems as though we were delinquent in changing our reagents ... that solved the problem. The wet noodle has been activated :) Much thanks . Gary From nbroadbent <@t> ucsd.edu Thu Dec 6 13:35:06 2007 From: nbroadbent <@t> ucsd.edu (Nicola J. Broadbent Ph.D) Date: Thu Dec 6 13:33:05 2007 Subject: [Histonet] Re:Interrupted thionin staining Message-ID: <003a01c8383f$1bc1cd00$8a4cef84@Nicola> Thank you to everyone who responded to my request regarding thionin staining and zylene. I appreciate all the advice! The staining worked out great! Nicola Nicola J. Broadbent Ph.D, Asst. Project Scientist Department of Psychiatry 0603, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA, 92093-0603 Email: nbroadbent@ucsd.edu Phone: (858) 642 3628 or (858) 552 8585 x 7853 Fax: (858) 552 7457 From failm <@t> musc.edu Thu Dec 6 14:07:32 2007 From: failm <@t> musc.edu (Fail, Mildred M.) Date: Thu Dec 6 14:09:36 2007 Subject: [Histonet] Re: Slide quality In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F2DD@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F2DD@wahtntex2.waht.swest.nhs.uk> Message-ID: More emphasis is placed on quantity rather than quality. Here the main histology lab is responsible for cutting everthing but controls for IHC/SS. Attempts to catch bad slides before staining were met with contempt, even though my superior had directed me to check the slides. Quite frankly I had to stop checking the slides, it just created too much animosity. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, December 06, 2007 4:59 AM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Robert I'm surprised that you don't look at the slides with the Tech; how does he/ she know if they are doing a good job. In my experience when my Pathologist was kind enough to teach me some smatterings of Dermatopathology it was stunningly powerful in showing me the error of my ways. I don't understand why Techs/ BMSs don't look at slides before they go to the Pathologist and even take a crack at the diagnosis. On one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't spotted by the Pathologist but then I was a Cytologist; wouldn't be any good at anything non- cervical; but I could learn!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MITCHELLJA <@t> email.chop.edu Thu Dec 6 14:21:59 2007 From: MITCHELLJA <@t> email.chop.edu (Janice Mitchell) Date: Thu Dec 6 14:21:32 2007 Subject: [Histonet] What is the CAP required water quality for a histology lab? We have been told we need to test our D Message-ID: What is the CAP required water quality for a histology lab? We have been told we need to test our DI water monthly but no one seems to know what quality level our water should be. Thanks, Janice From AnthonyH <@t> chw.edu.au Thu Dec 6 15:05:21 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Dec 6 15:06:54 2007 Subject: [Histonet] Re: Slide quality Message-ID: A good histotechnologists must be able to recognise tissue types. They must know when a section is adequate (nay should I say perfect?) for diagnosis. In my laboratories, my staff hate it when a medico tells them that a section is inadequate. Hey we know more about the science of Histopathology than a medico, that's why we are scientists or technicians. Granted some things slip through since we can only check a sample of the days work but without basic histology knowledge how can one differentiate a trichrome, or elastc stain? Solving problems is our job. Take the GI Bx problem. We have used a Lieca caroussel-type processor for many years to process these. It is a lot gentler on the tissue but for placenta and large thick tissue blocks we use a Shandon enclosed processor (more umph!). Train your staff to recognise good sections, give them spot quizzes on unknown tissues. More importantly when a problem is noted and you know how to solve it, don't. Let your staff knuckle it out. Eg, the eosin staining tray in the automatic stainer was low and only part of the section stained, yet the previous run with a full rack of slides was OK. Why? You will be surprised at some of the answers. Here is where you show your staff how to solve the problems. It gets them thinking. It provides a work environment that is interesting since to learn something new everyday, especially when they don't realise they are doing it, really can induce a productive and responsive workforce. Interesting I even wonder at some of our own ?experts in our External QAP. An assessment of a Perl's stain (stained in a coplin jar, not on a rack) stated that staining was uneven across the section. On review, it was noted that the slide stained (supplied by the QAP) was of uneven thickness. Couldn't the assessors pick this up? Anyway enough flamming before I get into real trouble!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, 6 December 2007 8:59 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Robert I'm surprised that you don't look at the slides with the Tech; how does he/ she know if they are doing a good job. In my experience when my Pathologist was kind enough to teach me some smatterings of Dermatopathology it was stunningly powerful in showing me the error of my ways. I don't understand why Techs/ BMSs don't look at slides before they go to the Pathologist and even take a crack at the diagnosis. On one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't spotted by the Pathologist but then I was a Cytologist; wouldn't be any good at anything non- cervical; but I could learn!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From scoop <@t> mail.nih.gov Thu Dec 6 16:42:21 2007 From: scoop <@t> mail.nih.gov (Sharon) Date: Thu Dec 6 16:42:51 2007 Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person Message-ID: Dear Histonetters, I have some questions about Nissl staining - (I have looked these issues up in histology texts but am still confused because I have received contradictory information - please clarify) Here are my ridiculous questions: 1. Is crystal violet ever used to stain epon embedded tissue for pathology? (I don't think so) 2. Is toluidine blue used as a Nissl stain? (I think so) 3. Are there advantages to Nissl staining with Cresyl Violet vs. Toluidine blue? (when you are just looking for pathology with no other stain - eg. not using the Toluidine blue or Cresyl Violet as a counter stain for some other stain). 4. Is Cresyl Violet used for anything other than a Nissl stain in brain? Thanks for your indulgence. Sharon From bob.nienhuis <@t> gmail.com Thu Dec 6 19:26:23 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Thu Dec 6 19:26:35 2007 Subject: [Histonet] Golgi slide bubbles Message-ID: <45109da50712061726t4434605ak1396208e3039d7@mail.gmail.com> Doing Golgi-Cox staining of mouse brains and am having a problem with bubbles appearing under the coverslip after va few days. I'm guessing it's due to the solvent evaporating from the thick mounting medium. Sections were cut frozen using the Kolb and McClimans (1986) cryostat technique. 100 micron sections, DPX mounting medium. Any suggestions? Bob Nienhuis From jnocito <@t> satx.rr.com Thu Dec 6 21:57:59 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 6 21:58:12 2007 Subject: [Histonet] What is the CAP required water quality for a histology lab? We have been told we need to test our D References: Message-ID: <005201c83885$5bf33350$0302a8c0@yourxhtr8hvc4p> Janice, as luck would have it, I just happen to have the new checklist in front of me (no, really, I do) CAP revised this requirement on the new 9/27/07 edition GEN.41500 Clinical Laboratory Reagent Water (CLRW) is the old Type I water which CAP has graciously enclosed in the checklist. Special Reagent Water (SRW) is now defined by laboratory procedures that need different specifications than CLRW, but CAP does not graciously enclose that requirement Instrument Feed Water (IFW) is determined by the equipment manufacturer. They reference the CLSI Guideline for information on special reagent water, whatever that means. Glad I could clear that up for you. No need to thank me, really. Now, I have to go find the CLSI Guideline myself. And people wonder why I have a problem with CAP. They couldn't leave well enough alone, could they? JTT ----- Original Message ----- From: "Janice Mitchell" To: Sent: Thursday, December 06, 2007 2:21 PM Subject: [Histonet] What is the CAP required water quality for a histology lab? We have been told we need to test our D > What is the CAP required water quality for a histology lab? We have > been told we need to test our DI water monthly but no one seems to know > what quality level our water should be. Thanks, Janice > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From daliaelrouby <@t> hotmail.com Thu Dec 6 23:00:33 2007 From: daliaelrouby <@t> hotmail.com (Dalia El Rouby) Date: Thu Dec 6 23:00:46 2007 Subject: [Histonet] bromephenol blue Message-ID: DEar Collegues: i am in urgent need for the detailed procedure for bromephenol blue stain used to detect protein. waiting for your kind reply _________________________________________________________________ News, entertainment and everything you care about at Live.com. Get it now! http://www.live.com/getstarted.aspx From shavaki <@t> med.uoa.gr Fri Dec 7 07:17:43 2007 From: shavaki <@t> med.uoa.gr (Sofia Havaki) Date: Fri Dec 7 07:14:29 2007 Subject: [Histonet] CK8 immunofluorescence problem Message-ID: <00f301c838d3$8d6f0fa0$817386c3@Tom> Dear Histonetters, I applied immunofluorescence on primary cultures of breast cancer cells and on cells from the established cell line MDA-MB-231 for the localization of cytokeratin 8, using the primary monoclonal antibody human anti-cytokeratin 8, purchased from Sigma (code No: C5301). My problem is that the immunofluorescence protocol that I followed, gave perfect results in the breast cancer cells of the culture cell line, while in the cells of the primary culture it didn't work (the immunofluorescence was very weak and obscure). The cells are permeabilized with Triton X-100 0.5 % diluted in PBS for 5min. Could the cells of the primary culture need different permeabilization time compared with those of the cell line? Could someone help?? Thank you in advance Sophia Havaki From valeria.berno <@t> embl.it Fri Dec 7 07:32:22 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Fri Dec 7 07:32:35 2007 Subject: [Histonet] c-fos Message-ID: <1806.10.251.1.146.1197034342.squirrel@10.251.1.146> Hi there, one of the user I am working with is having tough time to found a good antibody for c-fos to use in cryosection of mouse brain (30micron size) in immunofluorescence. Any suggestion? should she use a different protocol?The protocol right now is perfusion with PFA, sucrose, OCT,cutting, permebilization with 0.3%triton, blocking with serum and secondary A488. thanks in advance Valeria From asmith <@t> mail.barry.edu Fri Dec 7 09:00:05 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Dec 7 09:00:49 2007 Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person In-Reply-To: Message-ID: My cytology professor, Seymour Gelfant, recommended toluidine blue as a Nissl stain. Lillie's HISTOPATHOLOGIC TECHNIC also says that toluidine blue can be used as a Nissl stain. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Sent: Thursday, December 06, 2007 5:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person Dear Histonetters, I have some questions about Nissl staining - (I have looked these issues up in histology texts but am still confused because I have received contradictory information - please clarify) Here are my ridiculous questions: 1. Is crystal violet ever used to stain epon embedded tissue for pathology? (I don't think so) 2. Is toluidine blue used as a Nissl stain? (I think so) 3. Are there advantages to Nissl staining with Cresyl Violet vs. Toluidine blue? (when you are just looking for pathology with no other stain - eg. not using the Toluidine blue or Cresyl Violet as a counter stain for some other stain). 4. Is Cresyl Violet used for anything other than a Nissl stain in brain? Thanks for your indulgence. Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Dec 7 12:08:42 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Dec 7 12:09:18 2007 Subject: [Histonet] Golgi Cox mountant Message-ID: <8CA071355D14870-F48-7BC7@WEBMAIL-DC13.sysops.aol.com> Bob Nienhuis asks about a suitable mountant for Golgi Cox preparations: Years ago, when we did Golgi's we used Canada balsam as the mountant. It did not dry back as badly as synthetic mountants and was suitable for thick sections and things like parasite whole mounts. Trouble is, I don't know if it is still available. Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://o.aolcdn.com/cdn.webmail.aol.com/mailtour/aol/en-us/text.htm?ncid=aolcmp00050000000003 From koellingr <@t> comcast.net Fri Dec 7 12:26:25 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Dec 7 12:26:39 2007 Subject: [Histonet] CK8 immunofluorescence problem Message-ID: <120720071826.39.47599051000D567E0000002722007637049D09020704040A0105@comcast.net> Sofia, I'm not so sure that you need to change your procedure so much as to first entertain the possibility that you are looking for a one-to-one-to one correspondence between what are apples-oranges-and banana's. In-vivo there is some established, if not dynamic, level of expression of proteins including CK8. Pull those cells out and put them in primary culture and they can lose (or gain) some expressions. The very methodology you use to start the primary culture can cause loss of expression. And even if you hold the primary culture long term, no matter what you add to the culture in-vitro will never exactly parallel its in-vivo environment. The MDA's are still even more different being "not normal" in their very transformed existence. I've used MDA'a a lot and although not for CK8 expression, can tell you that what they express can certainly be different from the primary cells of their derivation or from in-vivo expression. There are ways t o check if you actually have the same " amounts" of CK8 between MDA-231's and primary cells from a more molecular sense. It is harder than tweaking a protocol but that is where I would search first. "Very weak and obscure" CK8 in primary cells compared to primary culture cells may be true biological fact. For many things I looked for in MDA's, that was the case. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: "Sofia Havaki" > Dear Histonetters, > > > > I applied immunofluorescence on primary cultures of breast cancer cells and on > cells from the established cell line MDA-MB-231 for the localization of > cytokeratin 8, using the primary monoclonal antibody human anti-cytokeratin 8, > purchased from Sigma (code No: C5301). My problem is that the immunofluorescence > protocol that I followed, gave perfect results in the breast cancer cells of the > culture cell line, while in the cells of the primary culture it didn't work (the > immunofluorescence was very weak and obscure). > > The cells are permeabilized with Triton X-100 0.5 % diluted in PBS for 5min. > Could the cells of the primary culture need different permeabilization time > compared with those of the cell line? > > > > Could someone help?? > > > > > > Thank you in advance > > Sophia Havaki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wstover <@t> vet.uga.edu Fri Dec 7 12:37:23 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Fri Dec 7 12:37:38 2007 Subject: [Histonet] UGA Workshop Message-ID: Reposting due to link failure: The University of Georgia will be hosting an immunohistochemistry workshop Jan. 12,2008. All that can register by Dec. 14, 2007 (but not necessary) will be greatly appreciated. See link for all info and to register. http://www.georgiacenter.uga.edu/conferences/2008/Jan/12/immuno.phtml Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From oshel1pe <@t> cmich.edu Fri Dec 7 12:40:37 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Dec 7 12:40:59 2007 Subject: [Histonet] Golgi slide bubbles In-Reply-To: <45109da50712061726t4434605ak1396208e3039d7@mail.gmail.com> References: <45109da50712061726t4434605ak1396208e3039d7@mail.gmail.com> Message-ID: Are these bubbles forming within the section, or intruding from the edges? If the former, all I can think of is Don Ho and "Tiny Bubbles". It's Friday and too few students are worrying about finishing their term projects. If the latter: I used to have this problem mounting 50 to 200 micron Golgi sections of goldfish brains. The solution was to put a 1/2 to 1 oz (14 to 28 g, keep it SIU) weight (fishing line weights work well) on the coverslip, and add mountant as needed to fill in the bubbles from the edge. Phil >Doing Golgi-Cox staining of mouse brains and am having a problem with >bubbles appearing under the coverslip after va few days. I'm >guessing it's due to the solvent evaporating from the thick mounting >medium. Sections were cut frozen using the Kolb and McClimans (1986) >cryostat technique. 100 micron sections, DPX mounting medium. > >Any suggestions? > >Bob Nienhuis -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From mcauliff <@t> umdnj.edu Fri Dec 7 13:13:01 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Dec 7 13:13:26 2007 Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person In-Reply-To: References: Message-ID: <47599B3D.2050106@umdnj.edu> HI Sharon: 1. Crystal violet does not enjoy wide use in pathology. However, you can use it any way you want. 2. Toluidine blue is a good Nissl stain but cresyl violet is more common. 3. Cresyl violet is more common, I suppose most prefer the violet color to blue. Cresyl violet gives better contrast with Luxol fast blue and I think cresyl violet is easier to differentiate with alcohols. 4. Probably. Geoff Are getting crystal violet and cresyl violet confused? Sharon wrote: > Dear Histonetters, > > I have some questions about Nissl staining - (I have looked these > issues up in histology texts but am still confused because I have > received contradictory information - please clarify) > Here are my ridiculous questions: > 1. Is crystal violet ever used to stain epon embedded tissue for > pathology? (I don't think so) > 2. Is toluidine blue used as a Nissl stain? (I think so) > 3. Are there advantages to Nissl staining with Cresyl Violet vs. > Toluidine blue? (when you are just looking for pathology with no other > stain - eg. not using the Toluidine blue or Cresyl Violet as a counter > stain for some other stain). > 4. Is Cresyl Violet used for anything other than a Nissl stain in brain? > > Thanks for your indulgence. > > Sharon > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From HDOWNS <@t> PARTNERS.ORG Fri Dec 7 13:14:01 2007 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Fri Dec 7 13:14:22 2007 Subject: [Histonet] Re: What is the CAP required water quality for a histology In-Reply-To: <6g2c7j$26irpr@phsmgmx9.partners.org> References: <6g2c7j$26irpr@phsmgmx9.partners.org> Message-ID: I think what they want you to do is have it cultured to make sure that there is no bacteria growing in it that could influence a stain. JACHO also has a requirement that our DI be sent to the micro lab once a month for culture, but we just supersede it by purchasing DI from a manufacturer, and then the quality control is on them not us. Heather Downs Nerve Injury Unit Massachusetts General Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, December 07, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 49, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Interrupted thionin staining (Nicola J. Broadbent Ph.D) 2. immunohitochem for IFN gamma (Mirela Hasu) 3. Re: reticulin/snooks (Philip Oshel) 4. Patchy staining (Martin, Gary) 5. Re:Interrupted thionin staining (Nicola J. Broadbent Ph.D) 6. RE: Re: Slide quality (Fail, Mildred M.) 7. What is the CAP required water quality for a histology lab? We have been told we need to test our D (Janice Mitchell) 8. RE: Re: Slide quality (Tony Henwood) 9. Nissl staining question: ignorant question from an ignorant person (Sharon) 10. Golgi slide bubbles (Bob Nienhuis) 11. Re: What is the CAP required water quality for a histology lab? We have been told we need to test our D (Joe Nocito) 12. bromephenol blue (Dalia El Rouby) 13. CK8 immunofluorescence problem (Sofia Havaki) 14. c-fos (Valeria Berno) 15. RE: Nissl staining question: ignorant question from an ignorant person (Smith, Allen) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Dec 2007 10:03:15 -0800 From: "Nicola J. Broadbent Ph.D" Subject: [Histonet] Interrupted thionin staining To: Message-ID: <002d01c83832$46d7b0c0$8a4cef84@Nicola> Content-Type: text/plain; charset="us-ascii" Hi, I would like to know whether it is ok to place rat brain tissue (50um slices on glass gelatin coated slides) directly from xylene to 100% alcholol to start the thionin staining process. Normally, we defat in a mix of chloroform and alcohol (approx 1 hr; which we did do with these brain sections) but due to being evacuated from our building we had to stop the staining process and in to store the tissue (as we were unsure how long we would be away from the lab) we placed the tissue in the zylene. The sections ended up being in zylene for approx 24 hrs. Would it be ok just to start the staining process from this point i.e. go straight to the 100%, 95% 70% alcohol steps from the zylene? Thanks so much for your help, Nicola Nicola J. Broadbent Ph.D, Asst. Project Scientist Department of Psychiatry 0603, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA, 92093-0603 Email: nbroadbent@ucsd.edu Phone: (858) 642 3628 or (858) 552 8585 x 7853 Fax: (858) 552 7457 ------------------------------ Message: 2 Date: Thu, 06 Dec 2007 13:33:35 -0500 From: "Mirela Hasu" Subject: [Histonet] immunohitochem for IFN gamma To: Message-ID: Content-Type: text/plain; charset=US-ASCII Hello, Does anybody have performed the immunohistochemistry for Interferon gamma on mice heart tissue and on aortic lesions. Would be very good if I can get a protocol that worked well. Thank you, Mirela ------------------------------ Message: 3 Date: Thu, 6 Dec 2007 13:34:00 -0500 From: Philip Oshel Subject: Re: [Histonet] reticulin/snooks To: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Cheri, The electron microscopy supply companies are the usual places for uranyl salts. These are Biological Stain Commission certified, but they are the pure (or nearly) powders. Electron Microscopy Supplies http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx Structure Probe, Inc. aka SPI http://www.2spi.com/catalog/chem/stain.shtml Also, regular chemical companies like Sigma-Aldrich have uranyl nitrate. http://www.sigmaaldrich.com/catalog/search/SearchResultsPage/PricingAvailability /FLUKA;94270 Phil >Who uses Snook's method for their retic stain?? If you do where do you get >your uranium nitrate?? I ask Rowley and they don't supply it anymore because >of it's hazards?? Am I missing something??? > >Cheri Miller HT ASCP > >Histology Supervisor >Physicians Laboratory Services, Inc. > >Omaha, NE 68117 > >402 738 5052 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 ------------------------------ Message: 4 Date: Thu, 6 Dec 2007 11:27:02 -0800 From: "Martin, Gary" Subject: [Histonet] Patchy staining To: Message-ID: <6ED9D4252F278841A0593D3D788AF24C0152E46B@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" Thank you to all who responded to my request for help concerning the voids/patchy staining. It seems as though we were delinquent in changing our reagents ... that solved the problem. The wet noodle has been activated :) Much thanks . Gary ------------------------------ Message: 5 Date: Thu, 6 Dec 2007 11:35:06 -0800 From: "Nicola J. Broadbent Ph.D" Subject: [Histonet] Re:Interrupted thionin staining To: Message-ID: <003a01c8383f$1bc1cd00$8a4cef84@Nicola> Content-Type: text/plain; charset="us-ascii" Thank you to everyone who responded to my request regarding thionin staining and zylene. I appreciate all the advice! The staining worked out great! Nicola Nicola J. Broadbent Ph.D, Asst. Project Scientist Department of Psychiatry 0603, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA, 92093-0603 Email: nbroadbent@ucsd.edu Phone: (858) 642 3628 or (858) 552 8585 x 7853 Fax: (858) 552 7457 ------------------------------ Message: 6 Date: Thu, 6 Dec 2007 15:07:32 -0500 From: "Fail, Mildred M." Subject: RE: [Histonet] Re: Slide quality To: "Kemlo Rogerson" , "Robert Richmond" , Message-ID: Content-Type: text/plain; charset="US-ASCII" More emphasis is placed on quantity rather than quality. Here the main histology lab is responsible for cutting everthing but controls for IHC/SS. Attempts to catch bad slides before staining were met with contempt, even though my superior had directed me to check the slides. Quite frankly I had to stop checking the slides, it just created too much animosity. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, December 06, 2007 4:59 AM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Robert I'm surprised that you don't look at the slides with the Tech; how does he/ she know if they are doing a good job. In my experience when my Pathologist was kind enough to teach me some smatterings of Dermatopathology it was stunningly powerful in showing me the error of my ways. I don't understand why Techs/ BMSs don't look at slides before they go to the Pathologist and even take a crack at the diagnosis. On one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't spotted by the Pathologist but then I was a Cytologist; wouldn't be any good at anything non- cervical; but I could learn!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 06 Dec 2007 15:21:59 -0500 From: "Janice Mitchell" Subject: [Histonet] What is the CAP required water quality for a histology lab? We have been told we need to test our D To: Message-ID: Content-Type: text/plain; charset=US-ASCII What is the CAP required water quality for a histology lab? We have been told we need to test our DI water monthly but no one seems to know what quality level our water should be. Thanks, Janice ------------------------------ Message: 8 Date: Fri, 7 Dec 2007 08:05:21 +1100 From: "Tony Henwood" Subject: RE: [Histonet] Re: Slide quality To: "Kemlo Rogerson" , "Robert Richmond" , Message-ID: Content-Type: text/plain; charset="us-ascii" A good histotechnologists must be able to recognise tissue types. They must know when a section is adequate (nay should I say perfect?) for diagnosis. In my laboratories, my staff hate it when a medico tells them that a section is inadequate. Hey we know more about the science of Histopathology than a medico, that's why we are scientists or technicians. Granted some things slip through since we can only check a sample of the days work but without basic histology knowledge how can one differentiate a trichrome, or elastc stain? Solving problems is our job. Take the GI Bx problem. We have used a Lieca caroussel-type processor for many years to process these. It is a lot gentler on the tissue but for placenta and large thick tissue blocks we use a Shandon enclosed processor (more umph!). Train your staff to recognise good sections, give them spot quizzes on unknown tissues. More importantly when a problem is noted and you know how to solve it, don't. Let your staff knuckle it out. Eg, the eosin staining tray in the automatic stainer was low and only part of the section stained, yet the previous run with a full rack of slides was OK. Why? You will be surprised at some of the answers. Here is where you show your staff how to solve the problems. It gets them thinking. It provides a work environment that is interesting since to learn something new everyday, especially when they don't realise they are doing it, really can induce a productive and responsive workforce. Interesting I even wonder at some of our own ?experts in our External QAP. An assessment of a Perl's stain (stained in a coplin jar, not on a rack) stated that staining was uneven across the section. On review, it was noted that the slide stained (supplied by the QAP) was of uneven thickness. Couldn't the assessors pick this up? Anyway enough flamming before I get into real trouble!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, 6 December 2007 8:59 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Slide quality You couldn't get the average pathologist, definitely including this one, to even notice most of the folds and wrinkles that occur in sections. We've become so used to ignoring them that when we teach photomicrography to residents, we have to remind them not to photograph areas in the slide with wrinkles in them - they look like hell when they're projected. I think that asking the pathologist to document wrinkles and folds is unreasonable. In the various labs I do pathology in, the recurrent problem is GI biopsies with shatter and "window-blind" artifact. My requests to address the problem are usually ignored. Very few pathology services do separate processor runs for small specimens, and I've never been able to get a laboratory to even consider it. If I ran the zoo, I'd have a double headed microscope (not permitted for pathologists in small pathology services), and I'd look at the day's run of slides with a senior histotechnologist nearly every day. That to my mind might launch an effective quality assurance program. I agree with you (before you argue with me!) that most pathologists in this circumstance would be so abusive that the exercise would be quite unendurable for the technologist. Robert I'm surprised that you don't look at the slides with the Tech; how does he/ she know if they are doing a good job. In my experience when my Pathologist was kind enough to teach me some smatterings of Dermatopathology it was stunningly powerful in showing me the error of my ways. I don't understand why Techs/ BMSs don't look at slides before they go to the Pathologist and even take a crack at the diagnosis. On one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't spotted by the Pathologist but then I was a Cytologist; wouldn't be any good at anything non- cervical; but I could learn!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 9 Date: Thu, 6 Dec 2007 17:42:21 -0500 From: Sharon Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person To: Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Dear Histonetters, I have some questions about Nissl staining - (I have looked these issues up in histology texts but am still confused because I have received contradictory information - please clarify) Here are my ridiculous questions: 1. Is crystal violet ever used to stain epon embedded tissue for pathology? (I don't think so) 2. Is toluidine blue used as a Nissl stain? (I think so) 3. Are there advantages to Nissl staining with Cresyl Violet vs. Toluidine blue? (when you are just looking for pathology with no other stain - eg. not using the Toluidine blue or Cresyl Violet as a counter stain for some other stain). 4. Is Cresyl Violet used for anything other than a Nissl stain in brain? Thanks for your indulgence. Sharon ------------------------------ Message: 10 Date: Thu, 6 Dec 2007 17:26:23 -0800 From: "Bob Nienhuis" Subject: [Histonet] Golgi slide bubbles To: Histonet Message-ID: <45109da50712061726t4434605ak1396208e3039d7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Doing Golgi-Cox staining of mouse brains and am having a problem with bubbles appearing under the coverslip after va few days. I'm guessing it's due to the solvent evaporating from the thick mounting medium. Sections were cut frozen using the Kolb and McClimans (1986) cryostat technique. 100 micron sections, DPX mounting medium. Any suggestions? Bob Nienhuis ------------------------------ Message: 11 Date: Thu, 6 Dec 2007 21:57:59 -0600 From: "Joe Nocito" Subject: Re: [Histonet] What is the CAP required water quality for a histology lab? We have been told we need to test our D To: "Janice Mitchell" , Message-ID: <005201c83885$5bf33350$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Janice, as luck would have it, I just happen to have the new checklist in front of me (no, really, I do) CAP revised this requirement on the new 9/27/07 edition GEN.41500 Clinical Laboratory Reagent Water (CLRW) is the old Type I water which CAP has graciously enclosed in the checklist. Special Reagent Water (SRW) is now defined by laboratory procedures that need different specifications than CLRW, but CAP does not graciously enclose that requirement Instrument Feed Water (IFW) is determined by the equipment manufacturer. They reference the CLSI Guideline for information on special reagent water, whatever that means. Glad I could clear that up for you. No need to thank me, really. Now, I have to go find the CLSI Guideline myself. And people wonder why I have a problem with CAP. They couldn't leave well enough alone, could they? JTT ----- Original Message ----- From: "Janice Mitchell" To: Sent: Thursday, December 06, 2007 2:21 PM Subject: [Histonet] What is the CAP required water quality for a histology lab? We have been told we need to test our D > What is the CAP required water quality for a histology lab? We have > been told we need to test our DI water monthly but no one seems to know > what quality level our water should be. Thanks, Janice > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 7 Dec 2007 07:00:33 +0200 From: Dalia El Rouby Subject: [Histonet] bromephenol blue To: Message-ID: Content-Type: text/plain; charset="windows-1256" DEar Collegues: i am in urgent need for the detailed procedure for bromephenol blue stain used to detect protein. waiting for your kind reply _________________________________________________________________ News, entertainment and everything you care about at Live.com. Get it now! http://www.live.com/getstarted.aspx ------------------------------ Message: 13 Date: Fri, 7 Dec 2007 15:17:43 +0200 From: "Sofia Havaki" Subject: [Histonet] CK8 immunofluorescence problem To: Message-ID: <00f301c838d3$8d6f0fa0$817386c3@Tom> Content-Type: text/plain; charset="iso-8859-7" Dear Histonetters, I applied immunofluorescence on primary cultures of breast cancer cells and on cells from the established cell line MDA-MB-231 for the localization of cytokeratin 8, using the primary monoclonal antibody human anti-cytokeratin 8, purchased from Sigma (code No: C5301). My problem is that the immunofluorescence protocol that I followed, gave perfect results in the breast cancer cells of the culture cell line, while in the cells of the primary culture it didn't work (the immunofluorescence was very weak and obscure). The cells are permeabilized with Triton X-100 0.5 % diluted in PBS for 5min. Could the cells of the primary culture need different permeabilization time compared with those of the cell line? Could someone help?? Thank you in advance Sophia Havaki ------------------------------ Message: 14 Date: Fri, 7 Dec 2007 14:32:22 +0100 (CET) From: "Valeria Berno" Subject: [Histonet] c-fos To: histonet@lists.utsouthwestern.edu Message-ID: <1806.10.251.1.146.1197034342.squirrel@10.251.1.146> Content-Type: text/plain;charset=iso-8859-1 Hi there, one of the user I am working with is having tough time to found a good antibody for c-fos to use in cryosection of mouse brain (30micron size) in immunofluorescence. Any suggestion? should she use a different protocol?The protocol right now is perfusion with PFA, sucrose, OCT,cutting, permebilization with 0.3%triton, blocking with serum and secondary A488. thanks in advance Valeria ------------------------------ Message: 15 Date: Fri, 7 Dec 2007 10:00:05 -0500 From: "Smith, Allen" Subject: RE: [Histonet] Nissl staining question: ignorant question from an ignorant person To: 'Sharon' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" My cytology professor, Seymour Gelfant, recommended toluidine blue as a Nissl stain. Lillie's HISTOPATHOLOGIC TECHNIC also says that toluidine blue can be used as a Nissl stain. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Sent: Thursday, December 06, 2007 5:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person Dear Histonetters, I have some questions about Nissl staining - (I have looked these issues up in histology texts but am still confused because I have received contradictory information - please clarify) Here are my ridiculous questions: 1. Is crystal violet ever used to stain epon embedded tissue for pathology? (I don't think so) 2. Is toluidine blue used as a Nissl stain? (I think so) 3. Are there advantages to Nissl staining with Cresyl Violet vs. Toluidine blue? (when you are just looking for pathology with no other stain - eg. not using the Toluidine blue or Cresyl Violet as a counter stain for some other stain). 4. Is Cresyl Violet used for anything other than a Nissl stain in brain? Thanks for your indulgence. Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 49, Issue 9 *************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From b-frederick <@t> northwestern.edu Fri Dec 7 15:22:55 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Dec 7 15:23:15 2007 Subject: [Histonet] Removing DAB Message-ID: <000001c83917$586c6930$d00f7ca5@lurie.northwestern.edu> Question: I need to restain slides for IHC. How do I get rid of DAB prior to restaining without killing the tissue? Tried a slide I did not need using DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the DAB out ,but also took the tissue. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From rjbuesa <@t> yahoo.com Fri Dec 7 15:55:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 7 15:55:42 2007 Subject: [Histonet] Removing DAB In-Reply-To: <000001c83917$586c6930$d00f7ca5@lurie.northwestern.edu> Message-ID: <769698.54559.qm@web61218.mail.yahoo.com> Once DAB reacts with the Ag-Ab-detection complex, "that's it", it cannot be removed without at least removing the IHC complex. Ren? J. Bernice Frederick wrote: Question: I need to restain slides for IHC. How do I get rid of DAB prior to restaining without killing the tissue? Tried a slide I did not need using DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the DAB out ,but also took the tissue. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mpence <@t> grhs.net Fri Dec 7 15:59:59 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Dec 7 16:00:15 2007 Subject: [Histonet] Removing DAB In-Reply-To: <000001c83917$586c6930$d00f7ca5@lurie.northwestern.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C7DF@IS-E2K3.grhs.net> As far as I know, you CANNOT restain once DAB has been on a tissue. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, December 07, 2007 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing DAB Question: I need to restain slides for IHC. How do I get rid of DAB prior to restaining without killing the tissue? Tried a slide I did not need using DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the DAB out ,but also took the tissue. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fong <@t> zoology.ubc.ca Fri Dec 7 16:05:48 2007 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Fri Dec 7 16:06:22 2007 Subject: [Histonet] c-fos In-Reply-To: <1806.10.251.1.146.1197034342.squirrel@10.251.1.146> References: <1806.10.251.1.146.1197034342.squirrel@10.251.1.146> Message-ID: <4759C3BC.1070406@zoology.ubc.ca> Hi, We've used a rabbit anti-c Fos antibody from Santa Cruz (SC-52) on rat brain tissue that has undergone similar protocol as what you describe. The immunofluorescent labelling (visualised with Cy3) worked well for us. Cheers Angelina > Hi there, > > one of the user I am working with is having tough time to found a good > antibody for c-fos to use in cryosection of mouse brain (30micron size) in > immunofluorescence. > > Any suggestion? should she use a different protocol?The protocol right now > is perfusion with PFA, sucrose, OCT,cutting, permebilization with > 0.3%triton, blocking with serum and secondary A488. > > thanks in advance > > Valeria > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5990 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From thisisann <@t> aol.com Fri Dec 7 17:52:46 2007 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Dec 7 17:53:03 2007 Subject: [Histonet] Supervisor Position in PA Message-ID: <8CA0743666E3BAC-3B4-41C6@webmail-de03.sysops.aol.com> I am currently looking for an experienced Histotechnologist, proficient in all aspects of Histology,? to run a small Histology laboratory in Rosemont, PA.? This person should possess a minimum of 5 years supervisory experience as well as the ability to work with minimal supervision. If you possess these skills, please e-mail your resume to?? ThisisAnn@aol.com. ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://o.aolcdn.com/cdn.webmail.aol.com/mailtour/aol/en-us/text.htm?ncid=aolcmp00050000000003 From gu.lang <@t> gmx.at Sat Dec 8 02:12:36 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Dec 8 02:12:51 2007 Subject: AW: [Histonet] Removing DAB In-Reply-To: <000001c83917$586c6930$d00f7ca5@lurie.northwestern.edu> Message-ID: <001a01c83972$181bf700$6412a8c0@dielangs.at> If you have the possibility take another kit, that results in red staining of the epitop. You have to do a double-stain in this case. The second has to be started with an heating step (HIER), that destroys all the antibodies from the first(DAB)part in the tissue. Then do ihc as usual and hopefully the red stain is not at the same place at the brown to differentiate the result. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bernice Frederick Gesendet: Freitag, 07. Dezember 2007 22:23 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Removing DAB Question: I need to restain slides for IHC. How do I get rid of DAB prior to restaining without killing the tissue? Tried a slide I did not need using DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the DAB out ,but also took the tissue. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.nixon <@t> beatson.gla.ac.uk Mon Dec 10 02:30:56 2007 From: c.nixon <@t> beatson.gla.ac.uk (Colin Nixon) Date: Mon Dec 10 02:31:12 2007 Subject: [Histonet] Processing schedule for mouse tissue. Message-ID: <0E5DFBD0E5E27443A0987F7664787163BAC890@exchange-be4.centre.ad.gla.ac.uk> Hi, I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor. 70% Alcohol 35 mins 90% Alcohol 40 mins 95% Alcohol 45 mins 100% Alcohol 60 mins 100% Alcohol 60 mins 100% Alcohol 60 mins Xylene 45 mins Xylene 60 mins Xylene 60 mins Wax 90 mins Wax 90 mins Wax 90 mins many thanks, Colin From kmerriam2003 <@t> yahoo.com Mon Dec 10 07:10:21 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Dec 10 07:10:34 2007 Subject: [Histonet] Processing schedule for mouse tissue. Message-ID: <114727.62674.qm@web50304.mail.re2.yahoo.com> I have a VIP, so I don't know about your specific processor; but I would decrease your times to about 30 minutes per station for the first 3 alchohols and to a total of about 90 minutes for the 100% alchohol, xylene and paraffin (for example, 30-30-30, or something like that). I am sure someone with your processor can chime in with a more specific mouse schedule. Good luck! Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Colin Nixon To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2007 3:30:56 AM Subject: [Histonet] Processing schedule for mouse tissue. Hi, I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor. 70% Alcohol 35 mins 90% Alcohol 40 mins 95% Alcohol 45 mins 100% Alcohol 60 mins 100% Alcohol 60 mins 100% Alcohol 60 mins Xylene 45 mins Xylene 60 mins Xylene 60 mins Wax 90 mins Wax 90 mins Wax 90 mins many thanks, Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From John.McGinley <@t> ColoState.EDU Mon Dec 10 09:13:04 2007 From: John.McGinley <@t> ColoState.EDU (McGinley,John) Date: Mon Dec 10 09:13:16 2007 Subject: [Histonet] Image analysis sample size Message-ID: Hi, I have a question regarding image analysis and sample size (microscopic fields). Our lab has been estimating proliferation indices in IHC stained (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x microscopic fields chosen at random, e.g. green dots placed on the tissue section prior to review under the scope in order to prevent selection bias toward heavily stained "hot spot" areas. We're using a CAS-200 imaging system that measures total nuclear area and DAB percent positive area above a specified threshold. The problem is that specimens can have a large range/variance depending upon the areas chosen for analysis and sampling 10 fields may not be enough. I'm thinking the best way to compensate for this problem would be to increase the number of fields until the variance stabilizes. Does anyone have an algorithm like an Excel macro or something similar that addresses this problem, i.e. will estimate how many more fields to count beyond 10 based on current variance? Thanks in advance. Regards, John N. McGinley Cancer Prevention Laboratory Colorado State University john.mcginley@colostate.edu From opiecurt <@t> yahoo.com Mon Dec 10 09:59:16 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Mon Dec 10 09:59:27 2007 Subject: [Histonet] tissue burnt/dried on processor Message-ID: <746070.67171.qm@web81602.mail.mud.yahoo.com> to summarize, we had some of the reagents errantly changed on the tissue processor. some of the 100% alc. seem to have been changed with 95% which we thing resulted in water in the tissue through the xylene and wax. however, the tissue was really dry and brittle.... i would really love to resolve this problem rather than tell the clients that their tissue is destroyed beyond repair. i have a picture i can forward to you if anyone would like to take a look. basically, the epi, on skins specifically, is staining really light. almost like we didn't leave it in there long enough? pathologists were not happy to say teh least. please help, let me know if i can forward the pic. curt --------------------------------- Never miss a thing. Make Yahoo your homepage. From schaundrawalton <@t> yahoo.com Mon Dec 10 10:14:29 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Mon Dec 10 10:14:41 2007 Subject: [Histonet] Cleaning Paraffin From Floors Message-ID: <751959.56571.qm@web58909.mail.re1.yahoo.com> Hey everybody! We are moving into a brand new lab in January 08. I got my first look at our new space last week and I'm concerned about the flooring they've selected for the new histo lab. It is vinyl. Which means we won't be able to scrape the paraffin that inevitably collects on the floors with our razor blade scraper. Does anyone have any suggestions? What are other people out there doing to prevent paraffin build-up on the floors? Thanks in advance! Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Samuel_Perry <@t> DFCI.HARVARD.EDU Mon Dec 10 10:16:37 2007 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Mon Dec 10 10:16:49 2007 Subject: [Histonet] IHC anti-vimentin on mouse tissue Message-ID: Hi All, I am having trouble finding a vimentin antibody that works well for IHC in mouse tissue. I have tried a goat anti-vimentin from Chemicon (AB1620) and a rabbit monoclonal anti-vimentin from Epitomics (cat. # 4211-1). The Chemicon had a lot of non-specific staining and the Epitomics showed no staining in mouse tissue. If anyone knows of a good IHC staining anti-vimentin antibody (preferably of rabbit specie) please let me know. Thanks -Sam The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From N.R.Reinders <@t> uu.nl Mon Dec 10 10:19:39 2007 From: N.R.Reinders <@t> uu.nl (Reinders, N.R. (Niels)) Date: Mon Dec 10 10:19:53 2007 Subject: [Histonet] Freezing methode Message-ID: <95D29B889F8EA44498550ECAA1C2763D0139E5FA@uu01msg-exb02.soliscom.uu.nl> Hello histosearch, I was wondering if anyone ever heard about a freezingmethode where a whole mouse-brain is placed is a cooled (nitrogen, dry-ice or methylbutane) brain matrix? Will it freeze to it not to be removed in one piece anymore? Or will it still change its shape in a matrix? I need an alternative because I want less-experienced people to be able to freeze the brain without much damage (I lost some brain lately). All idea's are welcome! Kind regards, N.Reinders N.Reinders, molecular analist, University of Utrecht, NL Yalelaan 2 3584 CM (363), Utrecht (0031) (0)30 2533818 N.R.Reinders@uu.nl From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Dec 10 10:21:45 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Dec 10 10:21:57 2007 Subject: [Histonet] tissue burnt/dried on processor Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F321@wahtntex2.waht.swest.nhs.uk> Odd that, if the tissue was not fully dehydrated then the xylene wouldn't have fully penetrated nor would the paraffin penetrated; it ought to be soggy not 'dry and burnt'. You could try revitalising by soaking in oil of cedar wood then reprocessesing but that's usually for 'overprocessed' rather than 'underprocessed' material. Interesting xylene, if that's what you use, can usually accommodate some water especially if fresh and clean (the xylene that is)and I have known tissue be successfully processed despite not using 100% and I wonder if the brittleness has been caused by something else; Too much heat? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From majid.ghoddusi <@t> gmail.com Mon Dec 10 10:49:48 2007 From: majid.ghoddusi <@t> gmail.com (Majid ghoddusi) Date: Mon Dec 10 10:50:04 2007 Subject: [Histonet] Job Opportunity in Silicon Valley - Histo Tech Message-ID: Comparative Biosciences Inc. has a full time position open for an experienced Research Associate or Scientist with proficiency in * Immunohistochemistry*. This researcher in this position will also perform in situ hybridization, cell culture and molecular techniques in of state of the art GLP histology laboratory. BS with demonstrable experience and capability will be considered. Flexible schedules are possible and our benefits are outstanding. Salary: $40,000 to $60,000 per year depending on qualifications and experience. Please visit our website: http://www.compbio.com/job-openings.html *Majid Ghoddusi, DVM, PhD* *Veterinary Pathologist* *Comparative Biosciences, Inc.* *786 Lucerne Drive, * *Sunnyvale, CA 94085* *http://www.compbio.com/* *Ph: 408-738-9265* *Fax: 408-738-9278* From andrew.wang <@t> tufts.edu Mon Dec 10 11:22:23 2007 From: andrew.wang <@t> tufts.edu (Andrew Wang) Date: Mon Dec 10 11:22:36 2007 Subject: [Histonet] CD11c on paraffin Message-ID: <6c0279d90712100922w4b6fe8ffnbdca8cafe9ae058@mail.gmail.com> Hi, I am hoping to stain human tissue embedded in paraffin for CD11c. Does anyone have any success with this protocol? Any help would be great for this newbie. Andrew Wang MSIV Department of Pathology Tufts University Sackler School of Graduate Biomedical Sciences University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School From bob.nienhuis <@t> gmail.com Mon Dec 10 11:36:50 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Mon Dec 10 11:37:06 2007 Subject: [Histonet] Image analysis sample size In-Reply-To: References: Message-ID: <45109da50712100936y1783be3p478a05a8a1084228@mail.gmail.com> You may want to do a sample power calculation. See: for an online version. Bob Nienhuis UCLA / VA Medical Center Los Angeles On Dec 10, 2007 7:13 AM, McGinley,John wrote: > Hi, > > I have a question regarding image analysis and sample size (microscopic > fields). Our lab has been estimating proliferation indices in IHC stained > (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x > microscopic fields chosen at random, e.g. green dots placed on the tissue > section prior to review under the scope in order to prevent selection bias > toward heavily stained "hot spot" areas. We're using a CAS-200 imaging > system that measures total nuclear area and DAB percent positive area above > a specified threshold. The problem is that specimens can have a large > range/variance depending upon the areas chosen for analysis and sampling 10 > fields may not be enough. I'm thinking the best way to compensate for this > problem would be to increase the number of fields until the variance > stabilizes. Does anyone have an algorithm like an Excel macro or something > similar that addresses this problem, i.e. will estimate how many more > fields to count beyond 10 based on current variance? Thanks in advance. > > Regards, > > John N. McGinley > Cancer Prevention Laboratory > Colorado State University > john.mcginley@colostate.edu > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From fudo <@t> ufl.edu Mon Dec 10 13:17:19 2007 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Mon Dec 10 13:17:36 2007 Subject: [Histonet] section on overfixed paraffin tissue Message-ID: <1223916757.447141197314239245.JavaMail.osg@osgjas04.cns.ufl.edu> Hi, all One of our histotechnician met a big problem on sectioning overfixed human and mouse pancreas. The tissues were overfixed in 4%NBF for several weeks before our PI gave to us for paraffin embedding and section. The histotechnician could not get intact pieces of the tissues because the sections started to break when they attach to the water(42-45C) in the waterbath. Does anyone have any experiences on sectioning overfixed tissues? Any suggestions? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology Core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From fudo <@t> ufl.edu Mon Dec 10 13:17:34 2007 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Mon Dec 10 13:17:46 2007 Subject: [Histonet] section on overfixed paraffin tissue Message-ID: <118832316.447171197314254422.JavaMail.osg@osgjas04.cns.ufl.edu> Hi, all One of our histotechnician met a big problem on sectioning overfixed human and mouse pancreas. The tissues were overfixed in 4%NBF for several weeks before our PI gave to us for paraffin embedding and section. The histotechnician could not get intact pieces of the tissues because the sections started to break when they attach to the water(42-45C) in the waterbath. Does anyone have any experiences on sectioning overfixed tissues? Any suggestions? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology Core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From mcauliff <@t> umdnj.edu Mon Dec 10 13:40:42 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Dec 10 13:40:59 2007 Subject: [Histonet] section on overfixed paraffin tissue In-Reply-To: <1223916757.447141197314239245.JavaMail.osg@osgjas04.cns.ufl.edu> References: <1223916757.447141197314239245.JavaMail.osg@osgjas04.cns.ufl.edu> Message-ID: <475D963A.40605@umdnj.edu> Formalin works slowly so "overfixation" is probably not the cause of the problem. Sounds like poor infiltration with wax to me. This could be secondary to poor dehydration and/or clearing. Geoff FU,DONGTAO wrote: > Hi, all > > One of our histotechnician met a big problem on sectioning overfixed > human and mouse pancreas. The tissues were overfixed in 4%NBF for > several weeks before our PI gave to us for paraffin embedding and > section. The histotechnician could not get intact pieces of the > tissues because the sections started to break when they attach to the > water(42-45C) in the waterbath. Does anyone have any experiences on > sectioning overfixed tissues? Any suggestions? > > Thank you, > > Ann Dongtao Fu MD, Ph.D > Lab Manager > Molecular Pathology Core > Dept. of Pathology > Lab phone: 352-273-7752 > Lab FAX: 352-273-7755 > Lab address: D11-50 > PO Box: 100275 > 1600 SW Archer Road > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From octavio109 <@t> hotmail.com Mon Dec 10 15:38:39 2007 From: octavio109 <@t> hotmail.com (Corinthia D. Emanuel) Date: Mon Dec 10 15:38:55 2007 Subject: [Histonet] RE: Histonet Digest, Vol 49, Issue 11 In-Reply-To: References: Message-ID: PLEASE ADD THE FOLOWING TO THE HISTONET SIITE. THANKS. Atlanta, GA, dermatology pathology lab seeks experienced histotechs and lab assistants. There are part time openings for early morning, late afternoon or evenings. Candidates must be available to work Saturday mornings between the hours of 5:00am - 12:00noon. ASAP, send email of interest and note derm path experience and exact availability (early mornings, evenings). Paste resume within the email, please. References will be required. Email to cemanuel@myfamilyderm.com > From: histonet-request@lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 49, Issue 11> To: histonet@lists.utsouthwestern.edu> Date: Mon, 10 Dec 2007 10:00:45 -0800> > Send Histonet mailing list submissions to> histonet@lists.utsouthwestern.edu> > To subscribe or unsubscribe via the World Wide Web, visit> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to> histonet-request@lists.utsouthwestern.edu> > You can reach the person managing the list at> histonet-owner@lists.utsouthwestern.edu> > When replying, please edit your Subject line so it is more specific> than "Re: Contents of Histonet digest..."> > > Today's Topics:> > 1. Processing schedule for mouse tissue. (Colin Nixon)> 2. Re: Processing schedule for mouse tissue. (Kim Merriam)> 3. Image analysis sample size (McGinley,John)> 4. tissue burnt/dried on processor (curt tague)> 5. Cleaning Paraffin From Floors (Schaundra Walton)> 6. IHC anti-vimentin on mouse tissue (Perry, Samuel)> 7. Freezing methode (Reinders, N.R. (Niels))> 8. RE: tissue burnt/dried on processor (Kemlo Rogerson)> 9. Job Opportunity in Silicon Valley - Histo Tech (Majid ghoddusi)> 10. CD11c on paraffin (Andrew Wang)> 11. Re: Image analysis sample size (Bob Nienhuis)> > > ----------------------------------------------------------------------> > Message: 1> Date: Mon, 10 Dec 2007 08:30:56 -0000> From: "Colin Nixon" > Subject: [Histonet] Processing schedule for mouse tissue.> To: > Message-ID:> <0E5DFBD0E5E27443A0987F7664787163BAC890@exchange-be4.centre.ad.gla.ac.uk>> > Content-Type: text/plain; charset="iso-8859-1"> > Hi,> > I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor.> > 70% Alcohol 35 mins> 90% Alcohol 40 mins> 95% Alcohol 45 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> Xylene 45 mins> Xylene 60 mins> Xylene 60 mins> Wax 90 mins> Wax 90 mins> Wax 90 mins> > many thanks,> > Colin > > > > > > > ------------------------------> > Message: 2> Date: Mon, 10 Dec 2007 05:10:21 -0800 (PST)> From: Kim Merriam > Subject: Re: [Histonet] Processing schedule for mouse tissue.> To: Colin Nixon ,> histonet@lists.utsouthwestern.edu> Message-ID: <114727.62674.qm@web50304.mail.re2.yahoo.com>> Content-Type: text/plain; charset=us-ascii> > I have a VIP, so I don't know about your specific processor; but I would decrease your times to about 30 minutes per station for the first 3 alchohols and to a total of about 90 minutes for the 100% alchohol, xylene and paraffin (for example, 30-30-30, or something like that). I am sure someone with your processor can chime in with a more specific mouse schedule.> > Good luck!> Kim> > Kim Merriam, MA, HT(ASCP)> Cambridge, MA> > > > ----- Original Message ----> From: Colin Nixon > To: histonet@lists.utsouthwestern.edu> Sent: Monday, December 10, 2007 3:30:56 AM> Subject: [Histonet] Processing schedule for mouse tissue.> > Hi,> > I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor.> > 70% Alcohol 35 mins> 90% Alcohol 40 mins> 95% Alcohol 45 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> Xylene 45 mins> Xylene 60 mins> Xylene 60 mins> Wax 90 mins> Wax 90 mins> Wax 90 mins> > many thanks,> > Colin > > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ____________________________________________________________________________________> Looking for last minute shopping deals? > Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping> > ------------------------------> > Message: 3> Date: Mon, 10 Dec 2007 08:13:04 -0700> From: "McGinley,John" > Subject: [Histonet] Image analysis sample size> To: "histonet@lists.utsouthwestern.edu"> , "ihcrg@googlegroups.com"> > Message-ID:> > Content-Type: text/plain; charset="us-ascii"> > Hi,> > I have a question regarding image analysis and sample size (microscopic fields). Our lab has been estimating proliferation indices in IHC stained (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x microscopic fields chosen at random, e.g. green dots placed on the tissue section prior to review under the scope in order to prevent selection bias toward heavily stained "hot spot" areas. We're using a CAS-200 imaging system that measures total nuclear area and DAB percent positive area above a specified threshold. The problem is that specimens can have a large range/variance depending upon the areas chosen for analysis and sampling 10 fields may not be enough. I'm thinking the best way to compensate for this problem would be to increase the number of fields until the variance stabilizes. Does anyone have an algorithm like an Excel macro or something similar that addresses this problem, i.e. will estimate how many more fields to count beyond 10 based on current variance? Thanks in advance.> > Regards,> > John N. McGinley> Cancer Prevention Laboratory> Colorado State University> john.mcginley@colostate.edu> > > > > > > > ------------------------------> > Message: 4> Date: Mon, 10 Dec 2007 07:59:16 -0800 (PST)> From: curt tague > Subject: [Histonet] tissue burnt/dried on processor> To: histonet@lists.utsouthwestern.edu> Message-ID: <746070.67171.qm@web81602.mail.mud.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1> > to summarize, we had some of the reagents errantly changed on the tissue processor. some of the 100% alc. seem to have been changed with 95% which we thing resulted in water in the tissue through the xylene and wax. however, the tissue was really dry and brittle.... i would really love to resolve this problem rather than tell the clients that their tissue is destroyed beyond repair. i have a picture i can forward to you if anyone would like to take a look. basically, the epi, on skins specifically, is staining really light. almost like we didn't leave it in there long enough? pathologists were not happy to say teh least.> > please help, let me know if i can forward the pic.> > curt> > > ---------------------------------> Never miss a thing. Make Yahoo your homepage.> > ------------------------------> > Message: 5> Date: Mon, 10 Dec 2007 08:14:29 -0800 (PST)> From: Schaundra Walton > Subject: [Histonet] Cleaning Paraffin From Floors> To: Histonet > Message-ID: <751959.56571.qm@web58909.mail.re1.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1> > Hey everybody!> > We are moving into a brand new lab in January 08. I got my first look at our new space last week and I'm concerned about the flooring they've selected for the new histo lab. It is vinyl. Which means we won't be able to scrape the paraffin that inevitably collects on the floors with our razor blade scraper. Does anyone have any suggestions? What are other people out there doing to prevent paraffin build-up on the floors?> > Thanks in advance!> > > > > Schaundra Walton BS HTL(ASCP)> Swedish American Hospital> 1401 E. State St. > Rockford, IL 61104> > ---------------------------------> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now.> > ------------------------------> > Message: 6> Date: Mon, 10 Dec 2007 11:16:37 -0500> From: "Perry, Samuel" > Subject: [Histonet] IHC anti-vimentin on mouse tissue> To: > Cc: "Chu, Gerald Chen,M.D.,Ph.D." > Message-ID:> > Content-Type: text/plain; charset="us-ascii"> > Hi All,> > I am having trouble finding a vimentin antibody that works well for IHC in mouse> tissue. I have tried a goat anti-vimentin from Chemicon (AB1620) and a rabbit> monoclonal anti-vimentin from Epitomics (cat. # 4211-1). The Chemicon had a lot> of non-specific staining and the Epitomics showed no staining in mouse tissue.> If anyone knows of a good IHC staining anti-vimentin antibody (preferably of> rabbit specie) please let me know. Thanks -Sam > > > The information transmitted in this electronic communication is intended only> for the person or entity to whom it is addressed and may contain confidential> and/or privileged material. Any review, retransmission, dissemination or other> use of or taking of any action in reliance upon this information by persons or> entities other than the intended recipient is prohibited. If you received this> information in error, please contact the Compliance HelpLine at 800-856-1983 and> properly dispose of this information.> > > > > ------------------------------> > Message: 7> Date: Mon, 10 Dec 2007 17:19:39 +0100> From: "Reinders, N.R. (Niels)" > Subject: [Histonet] Freezing methode> To: > Message-ID:> <95D29B889F8EA44498550ECAA1C2763D0139E5FA@uu01msg-exb02.soliscom.uu.nl>> > Content-Type: text/plain; charset="us-ascii"> > Hello histosearch,> > > > I was wondering if anyone ever heard about a freezingmethode where a> whole mouse-brain is placed is a cooled (nitrogen, dry-ice or> methylbutane) brain matrix? Will it freeze to it not to be removed in> one piece anymore? Or will it still change its shape in a matrix? I need> an alternative because I want less-experienced people to be able to> freeze the brain without much damage (I lost some brain lately). All> idea's are welcome!> > > > Kind regards, N.Reinders> > > > > > > > N.Reinders, molecular analist, > > University of Utrecht, NL> > Yalelaan 2 3584 CM (363), Utrecht> > (0031) (0)30 2533818> > N.R.Reinders@uu.nl > > > > ------------------------------> > Message: 8> Date: Mon, 10 Dec 2007 16:21:45 -0000> From: "Kemlo Rogerson" > Subject: RE: [Histonet] tissue burnt/dried on processor> To: "curt tague" ,> > Message-ID:> <86ADE4EB583CE64799A9924684A0FBBF0222F321@wahtntex2.waht.swest.nhs.uk>> Content-Type: text/plain; charset="us-ascii"> > Odd that, if the tissue was not fully dehydrated then the xylene> wouldn't have fully penetrated nor would the paraffin penetrated; it> ought to be soggy not 'dry and burnt'.> > You could try revitalising by soaking in oil of cedar wood then> reprocessesing but that's usually for 'overprocessed' rather than> 'underprocessed' material. Interesting xylene, if that's what you use,> can usually accommodate some water especially if fresh and clean (the> xylene that is)and I have known tissue be successfully processed despite> not using 100% and I wonder if the brittleness has been caused by> something else; Too much heat? > > > Kemlo Rogerson> Pathology Manager> DD 01934 647057 or extension 3311> Mob 07749 754194; Pager 07659 597107;> The road to success is dotted with many tempting parking places.> --Author Unknown > > This e-mail is confidential and privileged. If you are not the intended> recipient please accept my apologies; please do not disclose, copy or> distribute information in this e-mail or take any action in reliance on> its contents: to do so is strictly prohibited and may be unlawful.> Please inform me that this message has gone astray before deleting it.> Thank you for your co-operation> > > > > > ------------------------------> > Message: 9> Date: Mon, 10 Dec 2007 08:49:48 -0800> From: "Majid ghoddusi" > Subject: [Histonet] Job Opportunity in Silicon Valley - Histo Tech> To: histonet@lists.utsouthwestern.edu> Message-ID:> > Content-Type: text/plain; charset=ISO-8859-1> > Comparative Biosciences Inc. has a full time position open for an> experienced Research Associate or Scientist with proficiency in *> Immunohistochemistry*. This researcher in this position will also perform> in situ hybridization, cell culture and molecular techniques in of state of> the art GLP histology laboratory. BS with demonstrable experience and> capability will be considered. Flexible schedules are possible and our> benefits are outstanding. Salary: $40,000 to $60,000 per year depending on> qualifications and experience.> > > > Please visit our website: http://www.compbio.com/job-openings.html> > > > > > *Majid Ghoddusi, DVM, PhD*> *Veterinary Pathologist*> *Comparative Biosciences, Inc.*> *786 Lucerne Drive, *> *Sunnyvale, CA 94085*> *http://www.compbio.com/* > *Ph: 408-738-9265*> *Fax: 408-738-9278*> > > ------------------------------> > Message: 10> Date: Mon, 10 Dec 2007 12:22:23 -0500> From: "Andrew Wang" > Subject: [Histonet] CD11c on paraffin> To: histonet@lists.utsouthwestern.edu> Message-ID:> <6c0279d90712100922w4b6fe8ffnbdca8cafe9ae058@mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1> > Hi,> > I am hoping to stain human tissue embedded in paraffin for CD11c.> Does anyone have any success with this protocol? Any help would be> great for this newbie.> > Andrew Wang MSIV> Department of Pathology> Tufts University Sackler School of Graduate Biomedical Sciences> University of Medicine and Dentistry of New Jersey> Robert Wood Johnson Medical School> > > > ------------------------------> > Message: 11> Date: Mon, 10 Dec 2007 09:36:50 -0800> From: "Bob Nienhuis" > Subject: Re: [Histonet] Image analysis sample size> To: "McGinley,John" > Cc: "histonet@lists.utsouthwestern.edu"> , "ihcrg@googlegroups.com"> > Message-ID:> <45109da50712100936y1783be3p478a05a8a1084228@mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1> > You may want to do a sample power calculation.> See: > for an online version.> > Bob Nienhuis> UCLA / VA Medical Center> Los Angeles> > On Dec 10, 2007 7:13 AM, McGinley,John wrote:> > > Hi,> >> > I have a question regarding image analysis and sample size (microscopic> > fields). Our lab has been estimating proliferation indices in IHC stained> > (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x> > microscopic fields chosen at random, e.g. green dots placed on the tissue> > section prior to review under the scope in order to prevent selection bias> > toward heavily stained "hot spot" areas. We're using a CAS-200 imaging> > system that measures total nuclear area and DAB percent positive area above> > a specified threshold. The problem is that specimens can have a large> > range/variance depending upon the areas chosen for analysis and sampling 10> > fields may not be enough. I'm thinking the best way to compensate for this> > problem would be to increase the number of fields until the variance> > stabilizes. Does anyone have an algorithm like an Excel macro or something> > similar that addresses this problem, i.e. will estimate how many more> > fields to count beyond 10 based on current variance? Thanks in advance.> >> > Regards,> >> > John N. McGinley> > Cancer Prevention Laboratory> > Colorado State University> > john.mcginley@colostate.edu> >> >> >> >> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > > ------------------------------> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > End of Histonet Digest, Vol 49, Issue 11> **************************************** _________________________________________________________________ Put your friends on the big screen with Windows Vista? + Windows Live?. http://www.microsoft.com/windows/shop/specialoffers.mspx?ocid=TXT_TAGLM_CPC_MediaCtr_bigscreen_102007 From mickie25 <@t> netzero.net Mon Dec 10 17:36:09 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Mon Dec 10 17:37:03 2007 Subject: [Histonet] section on overfixed paraffin tissue In-Reply-To: <118832316.447171197314254422.JavaMail.osg@osgjas04.cns.ufl.edu> References: <118832316.447171197314254422.JavaMail.osg@osgjas04.cns.ufl.edu> Message-ID: HI Ann, This sounds to me a little like over-processed tissue, meaning over dehydrated and over cleared. Animal tissue especially is sensitive to becoming brittle. Under infiltration could be a problem, but check dehydration and clearing times. Good Luck Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Monday, December 10, 2007 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] section on overfixed paraffin tissue Hi, all One of our histotechnician met a big problem on sectioning overfixed human and mouse pancreas. The tissues were overfixed in 4%NBF for several weeks before our PI gave to us for paraffin embedding and section. The histotechnician could not get intact pieces of the tissues because the sections started to break when they attach to the water(42-45C) in the waterbath. Does anyone have any experiences on sectioning overfixed tissues? Any suggestions? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology Core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgdelaware <@t> comcast.net Mon Dec 10 18:01:43 2007 From: mgdelaware <@t> comcast.net (marian powers) Date: Mon Dec 10 18:01:55 2007 Subject: [Histonet] Histology position- Dover, DE Message-ID: Hello, We currently have an opening for an HT to work 2nd or 3rd shift. All inquiries contact mpowers@dpspa.com for more info. Thanks, Marian From Sarah.vanDamme <@t> esr.cri.nz Mon Dec 10 21:08:00 2007 From: Sarah.vanDamme <@t> esr.cri.nz (van Damme, Sarah) Date: Mon Dec 10 21:15:30 2007 Subject: [Histonet] Problems with Orange G staining Message-ID: <92B672A208AB2F419334EE1EC8C58D9DF62F6B@kscmail1.esr.cri.nz> Hi guys Is this the correct address to send questions to? If not, my apologies, but here it is if so: Hi I am using Dane's staining and failing to replicate some previously-obtained results for vaginal cells. The stain procedure is Mayer's haematoxylin, then bluing in lithium carbonate, then phloxin B, then Alcian blue, and last, orange G (colour index 16230). The vaginal samples originally came up orange, and buccal cells stained more pink/magenta. However, during the last few weeks, we have been unable to obtain these results: the vaginal cells are now mostly 'dusky pink', what I would call a light blue-based red. The buccal cells are now more orange. After trying a change to every variable I could think of, I managed to get the buccal cells staining more as they did in the previous staining runs (pink/magenta): however, the vaginal samples are not staining orange as they were previously. It seems to be a problem with the Orange G not working as it did before. Has anyone had experience with this stain not working in a similar situation, or does anyone have a suggestion? I am attempting to make sure that every variable is the same as it was during the time frame of the earlier results, right down to checking the source of the alcohol used for fixing slides, trying different lots of the stain powders, and the brand of the slides used. Any help is much appreciated. Sarah Van Damme Institute of Environmental and Scientific Research Auckland New Zealand P Think before you print This e-mail transmission and any attachments that accompany it may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law and is intended solely for the use of the individual(s) to whom it was intended to be addressed. If you have received this e-mail by mistake, or you are not the intended recipient, any disclosure, dissemination, distribution, copying or other use or retention of this communication or its substance is prohibited. If you have received this communication in error, please immediately reply to the author via e-mail that you received this message by mistake and also permanently delete the original and all copies of this e-mail and any attachments from your computer. Thank you. From jnocito <@t> satx.rr.com Tue Dec 11 06:07:29 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Dec 11 06:07:42 2007 Subject: [Histonet] tissue burnt/dried on processor References: <746070.67171.qm@web81602.mail.mud.yahoo.com> Message-ID: <00fc01c83bee$67304110$0302a8c0@yourxhtr8hvc4p> Curt, I had a problem with burnt tissue when I demo'ed a microwave tissue processor a couple of years ago. I cut the slides and used a HIER method just like on immunos. Some of the tissue stained better and some diagnosis's were able to be made, but some were totally lost. Hope this helps Joe ----- Original Message ----- From: "curt tague" To: Sent: Monday, December 10, 2007 9:59 AM Subject: [Histonet] tissue burnt/dried on processor > to summarize, we had some of the reagents errantly changed on the tissue > processor. some of the 100% alc. seem to have been changed with 95% which > we thing resulted in water in the tissue through the xylene and wax. > however, the tissue was really dry and brittle.... i would really love to > resolve this problem rather than tell the clients that their tissue is > destroyed beyond repair. i have a picture i can forward to you if anyone > would like to take a look. basically, the epi, on skins specifically, is > staining really light. almost like we didn't leave it in there long > enough? pathologists were not happy to say teh least. > > please help, let me know if i can forward the pic. > > curt > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpiche-grocki <@t> wtbyhosp.org Tue Dec 11 09:04:09 2007 From: jpiche-grocki <@t> wtbyhosp.org (Piche-Grocki, Jessica) Date: Tue Dec 11 09:05:23 2007 Subject: [Histonet] Thanks to everyone who responded about CDX2 and HCA Message-ID: <0A57D6AEAE4CBA4A984D27257160A72D01069D1D@win03exchange01.wtbyhosp.org> I appreciate everyones help in sharing their knowledge on CDX2 and HCA. Thanks again!! Jessica Piche-Grocki, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jpiche-grocki <@t> wtbyhosp.org Tue Dec 11 09:05:43 2007 From: jpiche-grocki <@t> wtbyhosp.org (Piche-Grocki, Jessica) Date: Tue Dec 11 09:08:44 2007 Subject: [Histonet] Embedding Cold Plate Quality Control Message-ID: <0A57D6AEAE4CBA4A984D27257160A72D01069D1E@win03exchange01.wtbyhosp.org> Hi All, Our lab manager wants to know what a temperature range should be for our cold plate on the embedding station. We can't seem to find any ranges in our Histology books so we are wondering what everyone else goes by. Thanks!! Jessica Piche-Grocki,HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Dec 11 09:38:09 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Dec 11 09:38:21 2007 Subject: [Histonet] Embedding Cold Plate Quality Control Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F332@wahtntex2.waht.swest.nhs.uk> Below the Eutectic point of water (4 degrees C) I guess; that point at which water is at its most dense, don't you think? If it becomes ice then it is less dense and I assume not as easy to section or would it be easier? Interesting point. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From mward <@t> wfubmc.edu Tue Dec 11 10:38:16 2007 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Dec 11 10:39:06 2007 Subject: [Histonet] RE: [IHCRG] commercially available antibody cocktail? In-Reply-To: <61E8DDCB5E48794283B9FADCE86FC946201266@wmhmail2.WHS.phci.org> References: <61E8DDCB5E48794283B9FADCE86FC946201266@wmhmail2.WHS.phci.org> Message-ID: <61135F0455D33347B5AAE209B903A3041F8943DB@EXCHVS2.medctr.ad.wfubmc.edu> We get ours from BioCare Medical. Martha Ward Wake Forest University Baptist Medical Center ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Tuesday, December 11, 2007 10:59 AM To: IHCRG Resource Group (E-mail) Subject: [IHCRG] commercially available antibody cocktail? Hi All, Does nayone out there know of a commercially available melanoma cocktail that contains Mart 1, HMB-45 and Tyrosinase? One of my Pathologists went to a meeting and they were talking about this antibody. Thanks Deb --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From geoffreymcook <@t> yahoo.com Tue Dec 11 11:03:45 2007 From: geoffreymcook <@t> yahoo.com (Geoff Cook) Date: Tue Dec 11 11:03:56 2007 Subject: [Histonet] Softening enrobed tissues` Message-ID: <271912.5315.qm@web50512.mail.re2.yahoo.com> Greetings! I am having problems sectioning samples of decalcified coral tissue (10% EDTA at neutral pH) that have been enrobed in 2% agarose. It appears that the enrobing media may be dehydrated. Consequently, the agarose seems to be "pulling away" from the paraffin in which the samples are embedded leaving visible holes and/or gaps. In addition, the consistency of the agarose appears to be a bit more brittle than the paraffin. Needless to say, sectioning these samples (5 um thickness) has been tricky. Can anyone offer a suggestion as to how this problem might be overcome? Is there a method/trick for softening the agarose while it is embedded in the parafin blocks? Many thanks in advance for your time. Aloha, Geoff Geoffrey M. Cook Department of Environmental Science and Policy George Mason University 4400 University Dr., MSN 5F2 Fairfax, Virginia 22030-4444 (office)?703.993.8917 (lab) 703.993.1219 gcook3@gmu.edu From pierre.chaumat <@t> alphelys.com Tue Dec 11 13:02:21 2007 From: pierre.chaumat <@t> alphelys.com (Pierre CHAUMAT) Date: Tue Dec 11 13:02:34 2007 Subject: [Histonet] Freezing methode In-Reply-To: <0MKpIi-1J1nKV20k4-0006vP@mx.kundenserver.de> Message-ID: <0MKwtQ-1J2AMw2cma-0003ZR@mrelayeu.kundenserver.de> Hello M. Reinders, One method to snapfreeze brains without much damages is to freeze into an isopentane bath cooled at -40?C. You may want to have a look at the Snapfrost machine (www.alphelys.com, section Specimen freezing) that controls precisely temperature and ensures a good and reproducible quality. Kind regards Pierre You wrote : Message: 7 Date: Mon, 10 Dec 2007 17:19:39 +0100 From: "Reinders, N.R. (Niels)" Subject: [Histonet] Freezing methode To: Message-ID: <95D29B889F8EA44498550ECAA1C2763D0139E5FA@uu01msg-exb02.soliscom.uu.nl> Content-Type: text/plain; charset="us-ascii" Hello histosearch, I was wondering if anyone ever heard about a freezingmethode where a whole mouse-brain is placed is a cooled (nitrogen, dry-ice or methylbutane) brain matrix? Will it freeze to it not to be removed in one piece anymore? Or will it still change its shape in a matrix? I need an alternative because I want less-experienced people to be able to freeze the brain without much damage (I lost some brain lately). All idea's are welcome! Kind regards, N.Reinders -----Message d'origine----- De : histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] De la part de histonet-request@lists.utsouthwestern.edu Envoy? : lundi 10 d?cembre 2007 19:26 ? : histonet@lists.utsouthwestern.edu Objet : Histonet Digest, Vol 49, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Processing schedule for mouse tissue. (Colin Nixon) 2. Re: Processing schedule for mouse tissue. (Kim Merriam) 3. Image analysis sample size (McGinley,John) 4. tissue burnt/dried on processor (curt tague) 5. Cleaning Paraffin From Floors (Schaundra Walton) 6. IHC anti-vimentin on mouse tissue (Perry, Samuel) 7. Freezing methode (Reinders, N.R. (Niels)) 8. RE: tissue burnt/dried on processor (Kemlo Rogerson) 9. Job Opportunity in Silicon Valley - Histo Tech (Majid ghoddusi) 10. CD11c on paraffin (Andrew Wang) 11. Re: Image analysis sample size (Bob Nienhuis) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Dec 2007 08:30:56 -0000 From: "Colin Nixon" Subject: [Histonet] Processing schedule for mouse tissue. To: Message-ID: <0E5DFBD0E5E27443A0987F7664787163BAC890@exchange-be4.centre.ad.gla.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Hi, I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor. 70% Alcohol 35 mins 90% Alcohol 40 mins 95% Alcohol 45 mins 100% Alcohol 60 mins 100% Alcohol 60 mins 100% Alcohol 60 mins Xylene 45 mins Xylene 60 mins Xylene 60 mins Wax 90 mins Wax 90 mins Wax 90 mins many thanks, Colin ------------------------------ Message: 2 Date: Mon, 10 Dec 2007 05:10:21 -0800 (PST) From: Kim Merriam Subject: Re: [Histonet] Processing schedule for mouse tissue. To: Colin Nixon , histonet@lists.utsouthwestern.edu Message-ID: <114727.62674.qm@web50304.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii I have a VIP, so I don't know about your specific processor; but I would decrease your times to about 30 minutes per station for the first 3 alchohols and to a total of about 90 minutes for the 100% alchohol, xylene and paraffin (for example, 30-30-30, or something like that). I am sure someone with your processor can chime in with a more specific mouse schedule. Good luck! Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Colin Nixon To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2007 3:30:56 AM Subject: [Histonet] Processing schedule for mouse tissue. Hi, I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor. 70% Alcohol 35 mins 90% Alcohol 40 mins 95% Alcohol 45 mins 100% Alcohol 60 mins 100% Alcohol 60 mins 100% Alcohol 60 mins Xylene 45 mins Xylene 60 mins Xylene 60 mins Wax 90 mins Wax 90 mins Wax 90 mins many thanks, Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________ ________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping ------------------------------ Message: 3 Date: Mon, 10 Dec 2007 08:13:04 -0700 From: "McGinley,John" Subject: [Histonet] Image analysis sample size To: "histonet@lists.utsouthwestern.edu" , "ihcrg@googlegroups.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I have a question regarding image analysis and sample size (microscopic fields). Our lab has been estimating proliferation indices in IHC stained (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x microscopic fields chosen at random, e.g. green dots placed on the tissue section prior to review under the scope in order to prevent selection bias toward heavily stained "hot spot" areas. We're using a CAS-200 imaging system that measures total nuclear area and DAB percent positive area above a specified threshold. The problem is that specimens can have a large range/variance depending upon the areas chosen for analysis and sampling 10 fields may not be enough. I'm thinking the best way to compensate for this problem would be to increase the number of fields until the variance stabilizes. Does anyone have an algorithm like an Excel macro or something similar that addresses this problem, i.e. will estimate how many more fields to count beyond 10 based on current variance? Thanks in advance. Regards, John N. McGinley Cancer Prevention Laboratory Colorado State University john.mcginley@colostate.edu ------------------------------ Message: 4 Date: Mon, 10 Dec 2007 07:59:16 -0800 (PST) From: curt tague Subject: [Histonet] tissue burnt/dried on processor To: histonet@lists.utsouthwestern.edu Message-ID: <746070.67171.qm@web81602.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 to summarize, we had some of the reagents errantly changed on the tissue processor. some of the 100% alc. seem to have been changed with 95% which we thing resulted in water in the tissue through the xylene and wax. however, the tissue was really dry and brittle.... i would really love to resolve this problem rather than tell the clients that their tissue is destroyed beyond repair. i have a picture i can forward to you if anyone would like to take a look. basically, the epi, on skins specifically, is staining really light. almost like we didn't leave it in there long enough? pathologists were not happy to say teh least. please help, let me know if i can forward the pic. curt --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 5 Date: Mon, 10 Dec 2007 08:14:29 -0800 (PST) From: Schaundra Walton Subject: [Histonet] Cleaning Paraffin From Floors To: Histonet Message-ID: <751959.56571.qm@web58909.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hey everybody! We are moving into a brand new lab in January 08. I got my first look at our new space last week and I'm concerned about the flooring they've selected for the new histo lab. It is vinyl. Which means we won't be able to scrape the paraffin that inevitably collects on the floors with our razor blade scraper. Does anyone have any suggestions? What are other people out there doing to prevent paraffin build-up on the floors? Thanks in advance! Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 6 Date: Mon, 10 Dec 2007 11:16:37 -0500 From: "Perry, Samuel" Subject: [Histonet] IHC anti-vimentin on mouse tissue To: Cc: "Chu, Gerald Chen,M.D.,Ph.D." Message-ID: Content-Type: text/plain; charset="us-ascii" Hi All, I am having trouble finding a vimentin antibody that works well for IHC in mouse tissue. I have tried a goat anti-vimentin from Chemicon (AB1620) and a rabbit monoclonal anti-vimentin from Epitomics (cat. # 4211-1). The Chemicon had a lot of non-specific staining and the Epitomics showed no staining in mouse tissue. If anyone knows of a good IHC staining anti-vimentin antibody (preferably of rabbit specie) please let me know. Thanks -Sam The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. ------------------------------ Message: 7 Date: Mon, 10 Dec 2007 17:19:39 +0100 From: "Reinders, N.R. (Niels)" Subject: [Histonet] Freezing methode To: Message-ID: <95D29B889F8EA44498550ECAA1C2763D0139E5FA@uu01msg-exb02.soliscom.uu.nl> Content-Type: text/plain; charset="us-ascii" Hello histosearch, I was wondering if anyone ever heard about a freezingmethode where a whole mouse-brain is placed is a cooled (nitrogen, dry-ice or methylbutane) brain matrix? Will it freeze to it not to be removed in one piece anymore? Or will it still change its shape in a matrix? I need an alternative because I want less-experienced people to be able to freeze the brain without much damage (I lost some brain lately). All idea's are welcome! Kind regards, N.Reinders N.Reinders, molecular analist, University of Utrecht, NL Yalelaan 2 3584 CM (363), Utrecht (0031) (0)30 2533818 N.R.Reinders@uu.nl ------------------------------ Message: 8 Date: Mon, 10 Dec 2007 16:21:45 -0000 From: "Kemlo Rogerson" Subject: RE: [Histonet] tissue burnt/dried on processor To: "curt tague" , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F321@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" Odd that, if the tissue was not fully dehydrated then the xylene wouldn't have fully penetrated nor would the paraffin penetrated; it ought to be soggy not 'dry and burnt'. You could try revitalising by soaking in oil of cedar wood then reprocessesing but that's usually for 'overprocessed' rather than 'underprocessed' material. Interesting xylene, if that's what you use, can usually accommodate some water especially if fresh and clean (the xylene that is)and I have known tissue be successfully processed despite not using 100% and I wonder if the brittleness has been caused by something else; Too much heat? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 9 Date: Mon, 10 Dec 2007 08:49:48 -0800 From: "Majid ghoddusi" Subject: [Histonet] Job Opportunity in Silicon Valley - Histo Tech To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Comparative Biosciences Inc. has a full time position open for an experienced Research Associate or Scientist with proficiency in * Immunohistochemistry*. This researcher in this position will also perform in situ hybridization, cell culture and molecular techniques in of state of the art GLP histology laboratory. BS with demonstrable experience and capability will be considered. Flexible schedules are possible and our benefits are outstanding. Salary: $40,000 to $60,000 per year depending on qualifications and experience. Please visit our website: http://www.compbio.com/job-openings.html *Majid Ghoddusi, DVM, PhD* *Veterinary Pathologist* *Comparative Biosciences, Inc.* *786 Lucerne Drive, * *Sunnyvale, CA 94085* *http://www.compbio.com/* *Ph: 408-738-9265* *Fax: 408-738-9278* ------------------------------ Message: 10 Date: Mon, 10 Dec 2007 12:22:23 -0500 From: "Andrew Wang" Subject: [Histonet] CD11c on paraffin To: histonet@lists.utsouthwestern.edu Message-ID: <6c0279d90712100922w4b6fe8ffnbdca8cafe9ae058@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I am hoping to stain human tissue embedded in paraffin for CD11c. Does anyone have any success with this protocol? Any help would be great for this newbie. Andrew Wang MSIV Department of Pathology Tufts University Sackler School of Graduate Biomedical Sciences University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School ------------------------------ Message: 11 Date: Mon, 10 Dec 2007 09:36:50 -0800 From: "Bob Nienhuis" Subject: Re: [Histonet] Image analysis sample size To: "McGinley,John" Cc: "histonet@lists.utsouthwestern.edu" , "ihcrg@googlegroups.com" Message-ID: <45109da50712100936y1783be3p478a05a8a1084228@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 You may want to do a sample power calculation. See: for an online version. Bob Nienhuis UCLA / VA Medical Center Los Angeles On Dec 10, 2007 7:13 AM, McGinley,John wrote: > Hi, > > I have a question regarding image analysis and sample size > (microscopic fields). Our lab has been estimating proliferation > indices in IHC stained (BrdU and Ki-67) rat mammary gland and mammary > tumors by evaluating ten 400x microscopic fields chosen at random, > e.g. green dots placed on the tissue section prior to review under the > scope in order to prevent selection bias toward heavily stained "hot > spot" areas. We're using a CAS-200 imaging system that measures total > nuclear area and DAB percent positive area above a specified > threshold. The problem is that specimens can have a large > range/variance depending upon the areas chosen for analysis and > sampling 10 fields may not be enough. I'm thinking the best way to > compensate for this problem would be to increase the number of fields > until the variance stabilizes. Does anyone have an algorithm like an > Excel macro or something similar that addresses this problem, i.e. will estimate how many more fields to count beyond 10 based on current variance? Thanks in advance. > > Regards, > > John N. McGinley > Cancer Prevention Laboratory > Colorado State University > john.mcginley@colostate.edu > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 49, Issue 11 **************************************** From laurie.colbert <@t> huntingtonhospital.com Tue Dec 11 16:40:22 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Dec 11 16:40:36 2007 Subject: [Histonet] Calibrated Measuring Devices Message-ID: <57BE698966D5C54EAE8612E8941D76830223690C@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can order metal cones and metal probes that you would insert into a vessel to measure the diameter of the vessel? Laurie Colbert From amosbrooks <@t> gmail.com Tue Dec 11 18:15:00 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Dec 11 18:15:12 2007 Subject: [Histonet] Glands in mouse fat Message-ID: <582736990712111615t36a06c28t3a01e8e4eab329ad@mail.gmail.com> Hi, I have a sample of adipose tissue from a mouse. The researcher described it as subdermal "deep fat" from the back of the mouse. The tissue appeared to have glands of some sort and I was having difficulty identifying it. The tissue is islands of glands surrounded in adipose. The 'glands' have a ring of cuboidal epithelium and a eosinophillic colloid of some sort inside. My experience is almost exclusively on human tissue so I'm a bit stumped. Initially I thought sweat gland or apocrine as it was near the skin, but (...duh) no sweat glands in mice. My next thought was that it had to be breast tissue. The histology looks the same (cuboidal epithelium, colloid, adipose) but the researcher says it is from the back of the mouse and he thinks the mouse was male. I asked if he was sure, it must have been a mouse with one heck of an identity crisis! So, I'll toss this into the ring. Does anyone have any ideas as to what this is. At this point it is really a matter of curiosity, but it is eating me up! I really should get an atlas of mouse anatomy & histology. Any suggestions? Thanks much, Amos Brooks From anh2006 <@t> med.cornell.edu Tue Dec 11 18:36:20 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Dec 11 18:36:38 2007 Subject: [Histonet] Glands in mouse fat In-Reply-To: <582736990712111615t36a06c28t3a01e8e4eab329ad@mail.gmail.com> References: <582736990712111615t36a06c28t3a01e8e4eab329ad@mail.gmail.com> Message-ID: Hi Amos, If it was a male I don't know what to tell you but female mice do have mammary glands that wrap around their sides onto their backs. Here is a picture of their anatomy which might help: http://eulep.pdn.cam.ac.uk/Necropsy_of_the_Mouse/imagesbig/Image7.jpg Good luck! Andrea At 7:15 PM -0500 12/11/07, Amos Brooks wrote: >Hi, > I have a sample of adipose tissue from a mouse. The researcher >described it as subdermal "deep fat" from the back of the mouse. The tissue >appeared to have glands of some sort and I was having difficulty identifying >it. The tissue is islands of glands surrounded in adipose. The 'glands' have >a ring of cuboidal epithelium and a eosinophillic colloid of some sort >inside. > My experience is almost exclusively on human tissue so I'm a bit >stumped. Initially I thought sweat gland or apocrine as it was near the >skin, but (...duh) no sweat glands in mice. My next thought was that it had >to be breast tissue. The histology looks the same (cuboidal epithelium, >colloid, adipose) but the researcher says it is from the back of the mouse >and he thinks the mouse was male. I asked if he was sure, it must have been >a mouse with one heck of an identity crisis! > So, I'll toss this into the ring. Does anyone have any ideas as to what >this is. At this point it is really a matter of curiosity, but it is eating >me up! I really should get an atlas of mouse anatomy & histology. Any >suggestions? > >Thanks much, >Amos Brooks -- From maa8 <@t> cornell.edu Wed Dec 12 00:07:51 2007 From: maa8 <@t> cornell.edu (Mary A. Ascenzi) Date: Wed Dec 12 00:09:01 2007 Subject: [Histonet] Glands in mouse fat In-Reply-To: <582736990712111615t36a06c28t3a01e8e4eab329ad@mail.gmail.com> References: <582736990712111615t36a06c28t3a01e8e4eab329ad@mail.gmail.com> Message-ID: Sounds like brown fat, found in mice and other small mammals and in hibernators. It is sometimes mistaken for a gland. Energy stored in brown fat is released as heat. Here is one website, but if you google "brown fat mouse" you can find some photomics and more info. www.vivo.colostate.edu/hbooks/ pathphys/misc_topics/brownfat Mary Ascenzi >Hi, > I have a sample of adipose tissue from a mouse. The researcher >described it as subdermal "deep fat" from the back of the mouse. The tissue >appeared to have glands of some sort and I was having difficulty identifying >it. The tissue is islands of glands surrounded in adipose. The 'glands' have >a ring of cuboidal epithelium and a eosinophillic colloid of some sort >inside. > My experience is almost exclusively on human tissue so I'm a bit >stumped. Initially I thought sweat gland or apocrine as it was near the >skin, but (...duh) no sweat glands in mice. My next thought was that it had >to be breast tissue. The histology looks the same (cuboidal epithelium, >colloid, adipose) but the researcher says it is from the back of the mouse >and he thinks the mouse was male. I asked if he was sure, it must have been >a mouse with one heck of an identity crisis! > So, I'll toss this into the ring. Does anyone have any ideas as to what >this is. At this point it is really a matter of curiosity, but it is eating >me up! I really should get an atlas of mouse anatomy & histology. Any >suggestions? > >Thanks much, >Amos Brooks >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Dec 12 02:36:10 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Dec 12 02:36:25 2007 Subject: [Histonet] manual IHC queries Message-ID: Hi all, Firstly: As a matter of interest - when using small amounts of primary antibody does anyone use coverslips to spread the solution over the section and keep it from evaporating during the incubation? (i do use a humidity chamber also). I have been doing this for ages and wondering if there is an alternative to use other than the coverslips that may be cheaper - any suggestions?? secondly, Although 30min incubations are recommended, is there any value to increasing incubation times of secondary and ABC complexes to improve sensitivity? In other words is there a maximal binding period after which no improvement will be noted? I hope I have made myself clear here....these are just passing wednesday thoughts in preparation for the usual friday meltdown! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From talulahgosh <@t> gmail.com Wed Dec 12 08:56:08 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Dec 12 08:56:20 2007 Subject: [Histonet] manual IHC queries In-Reply-To: References: Message-ID: When we use small amounts (80 ul/slide) of NBT/BCIP color reaction for in situs, we flip the slides upside down on parafilm in a humidity chamber. I don't know if it's any better than using a cover slip. Emily -- crow tom mike and joel trapped in space the endless void we've got movie sign! mst3k haiku brought to you by joe wiencis From fudo <@t> ufl.edu Wed Dec 12 09:09:19 2007 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Wed Dec 12 09:09:33 2007 Subject: [Histonet] Thanks for answering "section on overfixed paraffin tissue" email Message-ID: <10551080.449401197472159009.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, Thank you all for response to my question "section on overfixed paraffin tissue". I really appreciate. Have a nice day, Ann On Mon Dec 10 18:36:09 EST 2007, Mickie Johnson wrote: > HI Ann, > > This sounds to me a little like over-processed tissue, meaning > over > dehydrated and over cleared. Animal tissue especially is > sensitive to > becoming brittle. Under infiltration could be a problem, but > check > dehydration and clearing times. Good Luck > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: > mickie25@netzero.net > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > FU,DONGTAO > Sent: Monday, December 10, 2007 11:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] section on overfixed paraffin tissue > > Hi, all > > One of our histotechnician met a big problem on sectioning > overfixed human and mouse pancreas. The tissues were overfixed in > 4%NBF for several weeks before our PI gave to us for paraffin > embedding and section. The histotechnician could not get intact > pieces of the tissues because the sections started to break when > they attach to the water(42-45C) in the waterbath. Does anyone > have any experiences on sectioning overfixed tissues? Any > suggestions? > > Thank you, > > Ann Dongtao Fu MD, Ph.D > Lab Manager > Molecular Pathology Core > Dept. of Pathology > Lab phone: 352-273-7752 > Lab FAX: 352-273-7755 > Lab address: D11-50 > PO Box: 100275 > 1600 SW Archer Road > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From fudo <@t> ufl.edu Wed Dec 12 09:09:27 2007 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Wed Dec 12 09:09:41 2007 Subject: [Histonet] Thanks for answering "section on overfixed paraffin tissue" email Message-ID: <1364704900.449431197472167428.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, Thank you all for response to my question "section on overfixed paraffin tissue". I really appreciate. Have a nice day, Ann On Mon Dec 10 18:36:09 EST 2007, Mickie Johnson wrote: > HI Ann, > > This sounds to me a little like over-processed tissue, meaning > over > dehydrated and over cleared. Animal tissue especially is > sensitive to > becoming brittle. Under infiltration could be a problem, but > check > dehydration and clearing times. Good Luck > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: > mickie25@netzero.net > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > FU,DONGTAO > Sent: Monday, December 10, 2007 11:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] section on overfixed paraffin tissue > > Hi, all > > One of our histotechnician met a big problem on sectioning > overfixed human and mouse pancreas. The tissues were overfixed in > 4%NBF for several weeks before our PI gave to us for paraffin > embedding and section. The histotechnician could not get intact > pieces of the tissues because the sections started to break when > they attach to the water(42-45C) in the waterbath. Does anyone > have any experiences on sectioning overfixed tissues? Any > suggestions? > > Thank you, > > Ann Dongtao Fu MD, Ph.D > Lab Manager > Molecular Pathology Core > Dept. of Pathology > Lab phone: 352-273-7752 > Lab FAX: 352-273-7755 > Lab address: D11-50 > PO Box: 100275 > 1600 SW Archer Road > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From AWempren <@t> skcc.org Wed Dec 12 09:25:52 2007 From: AWempren <@t> skcc.org (Alexina Wempren) Date: Wed Dec 12 09:26:27 2007 Subject: [Histonet] antigen retrieval Message-ID: Hi all, I know this has probably been answered before but does anyone know the recipe for Tris-HCl pH 10? Thanks :-) From SBaldwin <@t> compucyte.com Wed Dec 12 10:24:52 2007 From: SBaldwin <@t> compucyte.com (Scott Baldwin) Date: Wed Dec 12 10:25:06 2007 Subject: [Histonet] Quantitative Imaging Cytometry Symposium Reminder Message-ID: To All Histonetters, This is a reminder to register for the Inaugural Quantitative Imaging Cytometry Symposium at the Cold Spring Harbor Laboratory, which is being offered as part of the CSHL Quantitative Imaging Cytometry Regional Center of Excellence. The symposium will take place at the Laboratory's Cold Spring Harbor, New York campus from January 30 to February 1, 2008. Space is limited, and therefore early registration is strongly advised. The meeting is structured to combine both the theory and practice of QIC, with morning seminars featuring a keynote lecturer and presentations by researchers employing the technology in their work. Afternoons will offer small group laboratory sessions, with extensive hands-on practical opportunities for further skill development in applying quantitative imaging cytometry (solid phase laser scanning cytometry) techniques in cell- and tissue-based applications. Confirmed morning presentations and speakers include: Day 1: Introduction to Quantitative Imaging Cytometry ? Time, Biochemistry, and Cell States: Role of cytometry in systems biology - James Jacobberger, Professor of Oncology, Case Comprehensive Cancer Center, Cleveland, OH ? Laser Scanning Cytometry: Where it fits into modern biomedical analysis - William Telford, NIH/NCI, Bethesda, MD ? Application of Laser Scanning Cytometry to the Functional Analysis of a Protein Phosphatase in a Mouse Model of Parkinson's Disease - Nicholas Tonks, Director of Shared Resources, Cold Spring Harbor Laboratory Cancer Center, Cold Spring Harbor, NY Day 2: Quantitative Imaging Cytometry for Cellular Analysis ? Quantitative Imaging Cytometry: How it complements the analytical capabilities of flow cytometry and molecular biology techniques - Zbigniew Darzynkiewicz, Professor of Pathology, Brander Cancer Research Institute, Valhalla, NY ? Cell Fate Signaling in Tumor Cells - Shazib Pervaiz, Professor, National University of Singapore ? Potential Mechanisms for the Generation of Chromosome Aneuploidy in Human Cancer - John M. Lehman, Professor of Pathology, Brody School of Medicine, East Carolina University, Greenville, NC ? The Role of Circulating Epithelial Tumor Cells (CETC) in the Metastatic Pathway - Katharina Pachmann, Professor, Department of Experimental Hematology and Oncology, Friedrich Schiller Universit?t, Jena, Germany Day 3: Quantitative Imaging Cytometry for Tissue Analysis ? Looking at Old Colors with New Lights: Applying quantitative imaging cytometry to diagnostic cytopathology and histopathology - William Geddie, Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada ? Quantitative Imaging Cytometry Applications in Pre-Clinical Drug Development - David Krull, GlaxoSmithKline Safety Assessment, Research Triangle Park, NC ? Quantitation of Caspase 3 Activation as a Pharmacodynamic Endpoint in Fine Needle Aspirate (FNA) Biopsies - Gloria Juan, Clinical Immunology, Amgen, Thousand Oaks, CA ? Quantitative Imaging of Biomarkers in Whole Sections and Tissue Microarrays - Viju Ananthanarayanan, University of Illinois at Chicago, IL Lectures are followed by small group laboratory sessions providing extensive hands-on practical opportunities for further skill development in applying quantitative imaging cytometry (solid phase laser scanning cytometry) techniques in cell- and tissue-based applications. These sessions will be guided by an expert-level faculty from universities and industry and will focus on the following areas: ? Practical instruction in designing QIC experiments: Implementation of high-content cell- and tissue-based applications on the iCys? Imaging Cytometer, quantification of fluorescence and laser light loss, defining assay end-points, dye selection (fluorescent and chromatic), sample preparation for automated analysis, troubleshooting analytical and image performance. ? Advanced data analysis techniques: Multiparameter cell cycle; DNA damage; high-content tissue microarray analysis. ? Individualized application development assistance on requested applications will be offered by experienced staff. A full 3-day course fee of $1500 will cover admission to the morning symposia, all afternoon practical sessions and materials, a peer-reviewed poster session and reception, accommodations for three nights and meals for three days on the Cold Spring Harbor Laboratory campus. Discounts are available for academic and government institutions. Partial registration packages are also available. Attendance at morning sessions only is priced at $50/day. Registration and a Call for Poster Abstracts are now available at www.ImagingCytometryCenter.com . Questions may be addressed to CSHL_Symposium@ImagingCytometryCenter.com or by phone at 612-202-8316. Scott Baldwin MT(ASCP) CompuCyte Corporation From mtarango <@t> nvcancer.org Wed Dec 12 10:53:19 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Dec 12 10:53:52 2007 Subject: [Histonet] manual IHC queries In-Reply-To: Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48C78@NVCIEXCH02.NVCI.org> I do sometimes use coverslips when doing IHC by hand. The humidity in Las Vegas is pretty low, so evaporation is always an issue. The Ventana's instruments use a coat of oil, over the aqueous antibody solution, to prevent evaporation. You could try using a lightweight mineral oil too. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, December 12, 2007 12:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] manual IHC queries Hi all, Firstly: As a matter of interest - when using small amounts of primary antibody does anyone use coverslips to spread the solution over the section and keep it from evaporating during the incubation? (i do use a humidity chamber also). I have been doing this for ages and wondering if there is an alternative to use other than the coverslips that may be cheaper - any suggestions?? secondly, Although 30min incubations are recommended, is there any value to increasing incubation times of secondary and ABC complexes to improve sensitivity? In other words is there a maximal binding period after which no improvement will be noted? I hope I have made myself clear here....these are just passing wednesday thoughts in preparation for the usual friday meltdown! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From settembr <@t> umdnj.edu Wed Dec 12 11:08:15 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Dec 12 11:08:59 2007 Subject: [Histonet] manual IHC queries Message-ID: Louise, I used to always use "Parafilm" and I sometimes still do. I cut it up into sizes that I need. It works marvelously and is cheap. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Tarango, Mark" 12/12/07 11:53 AM >>> I do sometimes use coverslips when doing IHC by hand. The humidity in Las Vegas is pretty low, so evaporation is always an issue. The Ventana's instruments use a coat of oil, over the aqueous antibody solution, to prevent evaporation. You could try using a lightweight mineral oil too. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, December 12, 2007 12:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] manual IHC queries Hi all, Firstly: As a matter of interest - when using small amounts of primary antibody does anyone use coverslips to spread the solution over the section and keep it from evaporating during the incubation? (i do use a humidity chamber also). I have been doing this for ages and wondering if there is an alternative to use other than the coverslips that may be cheaper - any suggestions?? secondly, Although 30min incubations are recommended, is there any value to increasing incubation times of secondary and ABC complexes to improve sensitivity? In other words is there a maximal binding period after which no improvement will be noted? I hope I have made myself clear here....these are just passing wednesday thoughts in preparation for the usual friday meltdown! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From valeria.berno <@t> embl.it Wed Dec 12 11:18:44 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Wed Dec 12 11:19:00 2007 Subject: [Histonet] nuclear staining Message-ID: <1946.10.251.1.146.1197479924.squirrel@10.251.1.146> Hi there! I am performing a 4 labeling immunofluorescent staining on seminifereous tubules. the antibodies I am working with are very good but the problem is the nuclear staining: I rather prefer toto-3 staining to the DAPI staining (it is easier to recognize the different stages of the cells). SO the option are: -to use the POPO-1(invitrogen) for nuclear staining:did someone ever try it? -to use TOTO-3 for nuclear staining and label one of the antibody with the Alexa350 (Zenon technology):did someone ever test this antibody on tissue? (I have a 405 laser). First of all thanks for all the past suggestion (always very useful) and I am sure you'll be great this time as well Valeria Berno Valeria, PhD EMBL- Mouse Biology Unit Campus A. Buzzati-Traverso Via Ramarini, 32 00015, Monterotondo Scalo (RM) Italy Tel: +39 06 90091287 Fax: +39 06 90091406 email: valeria.berno@embl.it www.embl.it From wstover <@t> vet.uga.edu Wed Dec 12 12:34:49 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Wed Dec 12 12:35:04 2007 Subject: [Histonet] Immunohistochemistry Workshop Message-ID: The University of Georgia will be hosting an immunohistochemistry workshop Jan. 12, 2008. If interest, please register by Dec.14, 2007 (but not necessary). Use this link to register and find all the info. Hope to see you there!! http://www.georgiacenter.uga.edu/conferences/2008/Jan/12/immuno.phtml Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From amylee779 <@t> yahoo.com Wed Dec 12 13:19:30 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Dec 12 13:19:41 2007 Subject: [Histonet] cassette labeling and slides labeling system Message-ID: <684757.14168.qm@web38002.mail.mud.yahoo.com> Hi histonetters, I am looking for a cassette labeling and slide labeling system. I know Shandon carry them. I am thinking having a package that both machines share same computer. Could any one give me any recommendation and any comment is highly appreciated. Amy --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Jackie.O'Connor <@t> abbott.com Wed Dec 12 13:34:32 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Dec 12 13:35:20 2007 Subject: [Histonet] cassette labeling and slides labeling system In-Reply-To: <684757.14168.qm@web38002.mail.mud.yahoo.com> Message-ID: Leica has, in my opinion, the best system - one computer runs both printers, and can print cassettes and slides for the same case/study simultaneously. Amy Lee Sent by: histonet-bounces@lists.utsouthwestern.edu 12/12/2007 01:19 PM To histonet cc Subject [Histonet] cassette labeling and slides labeling system Hi histonetters, I am looking for a cassette labeling and slide labeling system. I know Shandon carry them. I am thinking having a package that both machines share same computer. Could any one give me any recommendation and any comment is highly appreciated. Amy --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tthinnes <@t> scripps.edu Wed Dec 12 14:55:36 2007 From: tthinnes <@t> scripps.edu (terri thinnes) Date: Wed Dec 12 14:55:46 2007 Subject: [Histonet] Glands in mouse fat In-Reply-To: Message-ID: <000201c83d01$587ce1a0$21d48389@innateimmunweb> We have seen these structures. They looked like hair follicles to us. We saw them in subcutaneous fat and not in epididymal fat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary A. Ascenzi Sent: Tuesday, December 11, 2007 10:08 PM To: Amos Brooks; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Glands in mouse fat Sounds like brown fat, found in mice and other small mammals and in hibernators. It is sometimes mistaken for a gland. Energy stored in brown fat is released as heat. Here is one website, but if you google "brown fat mouse" you can find some photomics and more info. www.vivo.colostate.edu/hbooks/ pathphys/misc_topics/brownfat Mary Ascenzi >Hi, > I have a sample of adipose tissue from a mouse. The researcher >described it as subdermal "deep fat" from the back of the mouse. The tissue >appeared to have glands of some sort and I was having difficulty identifying >it. The tissue is islands of glands surrounded in adipose. The 'glands' have >a ring of cuboidal epithelium and a eosinophillic colloid of some sort >inside. > My experience is almost exclusively on human tissue so I'm a bit >stumped. Initially I thought sweat gland or apocrine as it was near the >skin, but (...duh) no sweat glands in mice. My next thought was that it had >to be breast tissue. The histology looks the same (cuboidal epithelium, >colloid, adipose) but the researcher says it is from the back of the mouse >and he thinks the mouse was male. I asked if he was sure, it must have been >a mouse with one heck of an identity crisis! > So, I'll toss this into the ring. Does anyone have any ideas as to what >this is. At this point it is really a matter of curiosity, but it is eating >me up! I really should get an atlas of mouse anatomy & histology. Any >suggestions? > >Thanks much, >Amos Brooks >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Dec 12 15:37:45 2007 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 12 15:56:00 2007 Subject: [Histonet] Control tissue for PCP Message-ID: > > Hi Histonetters. > > Some time ago, I read a procedure for growing your own Gram +/- controls using a piece of fresh liver. Has anyone tried something similar with lung tissue for PCP? The control slides are very expensive, so we're looking for an alternative. > > Thanks for any ideas you may have. > Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From Rcartun <@t> harthosp.org Wed Dec 12 16:30:18 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 12 16:30:41 2007 Subject: [Histonet] Control tissue for PCP In-Reply-To: References: Message-ID: <47601AAA0200007700009B3F@gwmail4.harthosp.org> I can send you a paraffin block if you send me your complete mailing address (USA only), telephone number, and an express mail account number (FedEx, UPS, etc.). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Rathborne, Toni" 12/12/07 4:37 PM >>> > > Hi Histonetters. > > Some time ago, I read a procedure for growing your own Gram +/- controls using a piece of fresh liver. Has anyone tried something similar with lung tissue for PCP? The control slides are very expensive, so we're looking for an alternative. > > Thanks for any ideas you may have. > Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From bob.nienhuis <@t> gmail.com Wed Dec 12 19:11:15 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Wed Dec 12 19:11:27 2007 Subject: [Histonet] Transferring frozen brain to fixative Message-ID: <45109da50712121711v5f9728afxd00ae39b8d7a9c29@mail.gmail.com> We have just received a frozen human hemisphere, presumably frozen in dry ice. The patient had a somewhat rare disorder. We want to transfer it to formalin to do IHC. We routinely do IHC on these brains using free-floating formalin fixed sections. Therefore, would have to freeze again to cut. Question: Best way to do this while minimizing damage 1. Thaw slowly in cold (4C) formalin for a long time in order to allow fix to penetrate? 2. Thaw quickly and then put in fix? 2. Try to block while frozen with a saw? 3. Other suggestions? Bob Nienhuis UCLA / VA Medical Center Los Angeles From tinayates <@t> comcast.net Thu Dec 13 00:30:14 2007 From: tinayates <@t> comcast.net (Tina Yates) Date: Thu Dec 13 00:30:28 2007 Subject: [Histonet] USED TISSUE PROCESSOR Message-ID: <021401c83d51$9f9b0080$4e8fab43@DFC4J821> Our lab is interested in purchasing a used Sakura VIP tissue processor. If you are an interested seller, please contact Shelly Siegel, HT(ASCP) Salem Hospital Regional Lab. P: 503.561.5443 shelly.siegel@salemhospital.org Thanks. From sonya.martin <@t> soton.ac.uk Thu Dec 13 08:31:17 2007 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Thu Dec 13 08:31:43 2007 Subject: [Histonet] Chemicals and pregnancy Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4135DA5BA@ISS-CL-EX-V1.soton.ac.uk> Hi All, I'm just wondering about the safety risks of working in an histology lab when pregnant. I have read through all the safety data and risk asessments for all the chemicals that I am currently using and there is nothing specific there about anything which may be harmful to the unborn child. I just wanted to check with you all to see what sort of precautions you would suggest. Mostly I just do IHC or fluorescence of frozen mouse tissues but I have the occasional paraffin embedded tissue to process as well. Thanks for your help Sonya From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Dec 13 08:42:16 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Dec 13 08:42:31 2007 Subject: [Histonet] Chemicals and pregnancy Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F357@wahtntex2.waht.swest.nhs.uk> I would not put a pregnant woman in the cut up room exposed to formalin, alcohols or any aromatic hydrocarbon. I would also avoid unfixed material and dry aniline dyes; anything with a mutagenic property ought to be avoided. Carry out a risk assessment and grade the risks; you then alter her working pattern to accommodate those risks you have now identified. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The road to success is dotted with many tempting parking places. --Author Unknown This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From PMcArdle <@t> ebsciences.com Thu Dec 13 09:15:19 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Thu Dec 13 09:15:36 2007 Subject: [Histonet] Chemicals and pregnancy In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4135DA5BA@ISS-CL-EX-V1.soton.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4135DA5BA@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <47614C87.4020203@ebsciences.com> Hi: I'd be concerned about xylene. The typical xylene MSDS sheet probably contains verbiage like the following: "Women may develop menstrual disorders, such as menorrhagia or metrorrhagia,infertility, and pathological pregnancy conditions including toxicosis, danger of miscarriage, and hemorrhaging during delivery. Repeated exposure of pregnant mice, rats and rabbits to the individual or the mixed isomers has resulted in maternal effects and effects on fertility, on the embryo or fetus, and specific developmental abnormalities. Included among these effects are fetal death, fetotoxicity, pre-and post-implantation mortality, abortion, craniofacial and musculoskeletal abnormalities, and extra embryonic structures." Getting xylene out of the lab was one compelling reason for use of microwave protocols which replace xylene with 2-propanol. Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. James S. wrote: > Hi All, > > I'm just wondering about the safety risks of working in an histology lab > when pregnant. I have read through all the safety data and risk > asessments for all the chemicals that I am currently using and there is > nothing specific there about anything which may be harmful to the unborn > child. I just wanted to check with you all to see what sort of > precautions you would suggest. > Mostly I just do IHC or fluorescence of frozen mouse tissues but I have > the occasional paraffin embedded tissue to process as well. > > Thanks for your help > Sonya > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Thu Dec 13 09:53:29 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Dec 13 09:53:42 2007 Subject: [Histonet] Listeria source In-Reply-To: <47614C87.4020203@ebsciences.com> References: <71437982F5B13A4D9A5B2669BDB89EE4135DA5BA@ISS-CL-EX-V1.soton.ac.uk> <47614C87.4020203@ebsciences.com> Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C401663A8@EXCHANGED.mgmt.ad.uconn.edu> Hello all, There seems to be a perpetual backorder for Difco's Listeria O Antibody. I've tried all the distributors. Can anyone suggest another Listeria antibody for Veterinary purposes? Thanks, Denise Long Woodward, MS, HT (ASCP), HTL, QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences 61 N. Eagleville Road, Unit 3089 Storrs, CT 06239-3089 From shive003 <@t> umn.edu Thu Dec 13 10:01:20 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Dec 13 10:01:34 2007 Subject: [Histonet] Listeria source References: <71437982F5B13A4D9A5B2669BDB89EE4135DA5BA@ISS-CL-EX-V1.soton.ac.uk><47614C87.4020203@ebsciences.com> <40AC6D73C2B95C4CA21B26B7BF380C401663A8@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: <00c701c83da1$66f684c0$b0065486@auxs.umn.edu> Biodesign; cat. # B65420R. Works very well. Jan Shivers Senior Scientist Histology/IHC/EM Section Leader University of Minnesota Veterinary Daignostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Woodward, Denise" To: Sent: Thursday, December 13, 2007 9:53 AM Subject: [Histonet] Listeria source Hello all, There seems to be a perpetual backorder for Difco's Listeria O Antibody. I've tried all the distributors. Can anyone suggest another Listeria antibody for Veterinary purposes? Thanks, Denise Long Woodward, MS, HT (ASCP), HTL, QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences 61 N. Eagleville Road, Unit 3089 Storrs, CT 06239-3089 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Thu Dec 13 10:07:01 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Thu Dec 13 10:07:31 2007 Subject: [Histonet] Mast cell identification Message-ID: <47610444.59BC.00AF.0@mercyhealth.com> Does anyone have experience using DAKO antibody-Mast Cell Tryptase? We have been using giemsa stains but my pathologist has asked me to look for something more definitive to identify and stain mast cells. If anyone could offer advice I would appreciate it. Thanks. Cindi Robinson, HT (ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 From Ronald.Houston <@t> nationwidechildrens.org Thu Dec 13 10:13:37 2007 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Dec 13 10:14:01 2007 Subject: [Histonet] Mast cell identification In-Reply-To: <47610444.59BC.00AF.0@mercyhealth.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB213F58BC4@chi2k3ms01.columbuschildrens.net> Not with Dako's antibody, but have used Novocastra's (cat# NCL-MCTRYP) at dilution of 1:640 after EDTA retrieval Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Robinson Sent: Thursday, December 13, 2007 11:07 AM To: histonet Subject: [Histonet] Mast cell identification Does anyone have experience using DAKO antibody-Mast Cell Tryptase? We have been using giemsa stains but my pathologist has asked me to look for something more definitive to identify and stain mast cells. If anyone could offer advice I would appreciate it. Thanks. Cindi Robinson, HT (ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From warhdlabreche <@t> shaw.ca Thu Dec 13 10:33:37 2007 From: warhdlabreche <@t> shaw.ca (Labreche Family) Date: Thu Dec 13 10:35:24 2007 Subject: [Histonet] Toluidine Blue Staining for BCC in Mohs Lab Message-ID: <2F8F9832-BFAA-45DC-8F15-F5C2055A2241@shaw.ca> Our newly acquired commercial 1%Toluidine Blue Stain is showing very pale metachromatic staining surrounding Basal Cell Cancer. I've been searching and reading various articles today on this site and others and am considering increasing the staining time further and dehydrating in the alcohols as quickly as possible before mounting in Clearium. Could the alkalinity of the stain be a problem? Any other ideas? We are in a small private Mohs lab in a medical clinic and basically dependant on commercial preparations. Anita L Edmonton,AB,Canada From shive003 <@t> umn.edu Thu Dec 13 10:41:55 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Dec 13 10:42:09 2007 Subject: [Histonet] Mast cell identification References: <47610444.59BC.00AF.0@mercyhealth.com> Message-ID: <00d801c83da7$125aea40$b0065486@auxs.umn.edu> Dako's anti-Mast Cell Tryptase works great. I use an HIER retrieval buffer that incorporates EDTA for 20 minutes (one of Dako's preparation). Jan Shivers Senior Scientist Histology/IHC/EM Section Leader University of Minnesota Veterinary Daignostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Cindy Robinson" To: "histonet" Sent: Thursday, December 13, 2007 10:07 AM Subject: [Histonet] Mast cell identification Does anyone have experience using DAKO antibody-Mast Cell Tryptase? We have been using giemsa stains but my pathologist has asked me to look for something more definitive to identify and stain mast cells. If anyone could offer advice I would appreciate it. Thanks. Cindi Robinson, HT (ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karenadams <@t> comcast.net Thu Dec 13 10:43:43 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Thu Dec 13 10:44:09 2007 Subject: [Histonet] pricing for embedding Message-ID: <121320071643.8245.4761613F000558500000203522070206539C030E0B0E020A9D0E05@comcast.net> is there a code (pricing) for 1. embedding formalin fixed tissues in prelabeled cassettes 2. same as above but samles arrive in containers need cassettes made. Thanks!! :) Happy almost Friday!!! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From eileen_dusek <@t> yahoo.com Thu Dec 13 10:50:45 2007 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Thu Dec 13 10:50:57 2007 Subject: [Histonet] Thank you for kidney bx information Message-ID: <850240.46982.qm@web50606.mail.re2.yahoo.com> Thanks to everyone for your help with my kidney bx situation. I am doing well, using tips from all of you. Have a safe Holiday season. Eileen --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Lynn.Burton <@t> Illinois.gov Thu Dec 13 11:52:15 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Dec 13 11:53:49 2007 Subject: [Histonet] Chemicals and pregnancy References: <71437982F5B13A4D9A5B2669BDB89EE4135DA5BA@ISS-CL-EX-V1.soton.ac.uk> Message-ID: Xylene and formalin are to be avoided as well as some of the stains. I remember that the crystal violet for a B&B was a high risk chemical. There are others. Lynn Burton Animal Disease Lab Galesburg,Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of James S. Sent: Thu 12/13/2007 8:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chemicals and pregnancy Hi All, I'm just wondering about the safety risks of working in an histology lab when pregnant. I have read through all the safety data and risk asessments for all the chemicals that I am currently using and there is nothing specific there about anything which may be harmful to the unborn child. I just wanted to check with you all to see what sort of precautions you would suggest. Mostly I just do IHC or fluorescence of frozen mouse tissues but I have the occasional paraffin embedded tissue to process as well. Thanks for your help Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Dec 13 13:30:56 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Dec 13 13:31:47 2007 Subject: [Histonet] Re: IHC anti-vimentin on mouse tissue Message-ID: <001701c83dbe$af18d560$4101a8c0@carlba65530bda> Are you referring to detection of Vimentin in Formalin-fixed pwax sections? If not, please ignore this. If yes, I have not yet come across any that work in mouse. (The V9 clone mouse monoclonal Ab is superb for rat) I will shortly test 2 anti-Vimentin Abs on mouse pwax sections. ( Abcam ab39376, ab45939) Carl From sbreeden <@t> nmda.nmsu.edu Thu Dec 13 14:42:51 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Dec 13 14:43:05 2007 Subject: [Histonet] 10% formaldehyde?? Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E60DE@nmdamailsvr.nmda.ad.nmsu.edu> Our receiving person just went to a shipping/receiving hazardous materials workshop where she was told that neither 10% FORMALIN nor 10% FORMALDEHYDE are considered "dangerous goods". What the heck? This will probably end up being my forehead-slapper of the day, but aren't we talking two different animals here? Our specimens come (well, usually) in 10%NBF (and sometimes Jack Daniels, I swear!) but is the statement that was made in the class assuming that "formaldehyde" and "formalin" are the same thing? Mind you, I was not in this class, but I have seen their notebook and one of the pages says "10% formaldehyde is not subject to the requirements as Division 6.2 material." I don't consider formaldehyde/formalin to be non-dangerous... It's okay for me to be confused, but not on a Thursday, so I need help. And I don't do the receiving, so I'm not up on all the shipping requirements. Thank you all! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From olek.michalski <@t> nencki.gov.pl Thu Dec 13 14:31:22 2007 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Thu Dec 13 14:48:58 2007 Subject: [Histonet] prolonged impregnation in Golgi-Cox (Kolb) staining Message-ID: Dear Histonetters, I have some brains which were left in Golgi-Cox chromation solution (K2Cr2O7, KCrO3, HgCl2: about 1% of each) for over 6 months. I would really like to use this material even if staining would not be very clear. Do you have an idea how to process the tissue in this case? Any help would be appreciated (and I know, next time I will be more careful about where I leave my preparates). By the way, I have already cut one of these brains and I found the sections to be dark green. I have noticed this colour in my G-C preparates before but mainly on the surface. I am actually curious whether the tissue should be more green or more red when I cut it. I mean should I shorten the normal period of chromation (about 14-15 days) if I see the brain is getting green? Yours truly Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From JCrofoot <@t> dow.com Thu Dec 13 15:03:17 2007 From: JCrofoot <@t> dow.com (Crofoot, Jacqueline (J)) Date: Thu Dec 13 15:03:48 2007 Subject: [Histonet] Antigen Retrieval Message-ID: <16E8141FECDB7C4DAA46DEC7039D07D10127BF7A@USMDLMDOWX027.dow.com> If anyone could recommend a method for antigen retrieval for Ki-67 on rat and mouse tissue that has been fixed in formalin for more than 90 days, I would really appreciate it. Jackie Crofoot Dow Chemical Co. 1803 Building Pathology Dept. Midland, MI 48674 Tx 989-636-3539 From aep10 <@t> cornell.edu Thu Dec 13 15:11:22 2007 From: aep10 <@t> cornell.edu (Anna Elisse Beaudin) Date: Thu Dec 13 15:11:37 2007 Subject: [Histonet] Antigen Retrieval In-Reply-To: <16E8141FECDB7C4DAA46DEC7039D07D10127BF7A@USMDLMDOWX027.dow.com> References: <16E8141FECDB7C4DAA46DEC7039D07D10127BF7A@USMDLMDOWX027.dow.com> Message-ID: <3434.128.253.96.73.1197580282.squirrel@webmail.cornell.edu> hi Jackie, I do 10 minutes in 95C citrate buffer pH6.0, followed by 20 minutes cooling at room temp. I'm working with 20 uM mouse embryo sections, and it seems to do the trick for Ki-67 immunohistochemistry. hope that helps, Anna If anyone could recommend a method for antigen retrieval for Ki-67 on > rat and mouse tissue that has been fixed in formalin for more than 90 > days, I would really appreciate it. > > Jackie Crofoot > Dow Chemical Co. > 1803 Building > Pathology Dept. > Midland, MI 48674 > Tx 989-636-3539 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Thu Dec 13 15:33:13 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Dec 13 15:33:25 2007 Subject: [Histonet] 10% formaldehyde?? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E60DE@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D08@LSRIEXCH1.lsmaster.lifespan.org> Technically formaldehyde is a gas. Formalin is a solution of formaldehyde gas in water. That's what we use in histology. What we usually refer to as "formaldehyde" is more properly called "formaldehyde solution", and the further dilutions of that stock formaldehyde solution is what we usually refer to as "formalin". From mpence <@t> grhs.net Thu Dec 13 15:44:59 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Dec 13 15:45:13 2007 Subject: [Histonet] 10% formaldehyde?? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E60DE@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C7FD@IS-E2K3.grhs.net> See this letter from the DOT web site www.phmsa.dot.gov/portal/site/PHMSA/menuitem.ebdc7a8a7e39f2e55cf20310502 48a0c/?vgnextoid=691dc0515d544110VgnVCM1000009ed07898RCRD&vgnextchannel= aa8cd3c1af814110VgnVCM1000009ed07898RCRD&vgnextfmt=print -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, December 13, 2007 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 10% formaldehyde?? Our receiving person just went to a shipping/receiving hazardous materials workshop where she was told that neither 10% FORMALIN nor 10% FORMALDEHYDE are considered "dangerous goods". What the heck? This will probably end up being my forehead-slapper of the day, but aren't we talking two different animals here? Our specimens come (well, usually) in 10%NBF (and sometimes Jack Daniels, I swear!) but is the statement that was made in the class assuming that "formaldehyde" and "formalin" are the same thing? Mind you, I was not in this class, but I have seen their notebook and one of the pages says "10% formaldehyde is not subject to the requirements as Division 6.2 material." I don't consider formaldehyde/formalin to be non-dangerous... It's okay for me to be confused, but not on a Thursday, so I need help. And I don't do the receiving, so I'm not up on all the shipping requirements. Thank you all! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ribonukle <@t> yahoo.com.ar Thu Dec 13 14:57:44 2007 From: Ribonukle <@t> yahoo.com.ar (ribonukle) Date: Thu Dec 13 15:45:32 2007 Subject: [Histonet] unsuscribe References: <582736990712111615t36a06c28t3a01e8e4eab329ad@mail.gmail.com> Message-ID: -------------------------------------------------- From: "Amos Brooks" Sent: Tuesday, December 11, 2007 9:15 PM To: Subject: [Histonet] Glands in mouse fat > Hi, > I have a sample of adipose tissue from a mouse. The researcher > described it as subdermal "deep fat" from the back of the mouse. The > tissue > appeared to have glands of some sort and I was having difficulty > identifying > it. The tissue is islands of glands surrounded in adipose. The 'glands' > have > a ring of cuboidal epithelium and a eosinophillic colloid of some sort > inside. > My experience is almost exclusively on human tissue so I'm a bit > stumped. Initially I thought sweat gland or apocrine as it was near the > skin, but (...duh) no sweat glands in mice. My next thought was that it > had > to be breast tissue. The histology looks the same (cuboidal epithelium, > colloid, adipose) but the researcher says it is from the back of the mouse > and he thinks the mouse was male. I asked if he was sure, it must have > been > a mouse with one heck of an identity crisis! > So, I'll toss this into the ring. Does anyone have any ideas as to > what > this is. At this point it is really a matter of curiosity, but it is > eating > me up! I really should get an atlas of mouse anatomy & histology. Any > suggestions? > > Thanks much, > Amos Brooks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.503 / Virus Database: 269.17.0/1180 - Release Date: > 10/12/2007 02:51 p.m. > From laurie <@t> conxis.com Thu Dec 13 17:56:22 2007 From: laurie <@t> conxis.com (Laurie Popp) Date: Thu Dec 13 17:57:14 2007 Subject: [Histonet] Re: Pregnancy Message-ID: <4761C6A6.2050207@conxis.com> Hi Histonetters, I actually just had a healthy baby girl 8/10/07 and was a high risk pregnancy ( diabetic) while finishing my HT and working full time in the histo lab at Mayo. I was advised by my OB to limit my formalin exposure, to limit my xylene exposure as much as possible, and to be very careful in special stains area. To also be careful of B-5 which we use for our bone marrows and the list goes on. Basically I was limited to cutting and embedding most days. Hope this helps! Laurie Popp, BA HT ( ASCP) and happy mommy to Kaiana :-) From leon.brokken <@t> med.lu.se Fri Dec 14 03:10:29 2007 From: leon.brokken <@t> med.lu.se (Leon Brokken) Date: Fri Dec 14 03:11:01 2007 Subject: [Histonet] Marker antibody for mast cells in prostate Message-ID: <47624885.3070203@med.lu.se> Hi all, could someone recommend a good antibody to identify mast cells in formalin-fixed paraffin-embedded prostate cancer tissue? Preferrably non-goat, for double labelling puproses. Cheers, Leon. -- Leon J.S. Brokken Tumour Biology, Dept. of Laboratory Medicine, Lund University UMAS, CRC, Entrance 72, House 91, Floor 10, 20502 Malm?, Sweden Tel. +46(0)40391104, +46(0)739531450 (gsm), Fax +46(0)40391222 -- GnuPG: 0x0099F279 | 2C0C 34AF 44B4 B836 251F 24F6 47EA 90A6 0099 F279 -- From taben.hale <@t> gmail.com Fri Dec 14 03:21:39 2007 From: taben.hale <@t> gmail.com (Taben Hale) Date: Fri Dec 14 03:21:50 2007 Subject: [Histonet] corpora cavernosal sections Message-ID: Does anyone have any experience with making penis (specifically cavernosal, paraffin embedded---I am using rat tissue) sections? I have tried 3micron and 5 micron sections, but I almost always get parts of the section that peel off of the slide, especially after antigen retrieval (citrate buffer, 95C). I have been somewhat more successful with APES coated slides, but I would welcome any and all suggestions. -- Taben M Hale, PhD Postdoctoral Fellow Universite de Montreal Dept Pharmacologie (514)343-6111 ext. 4968 From jnocito <@t> satx.rr.com Fri Dec 14 06:07:09 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Dec 14 06:09:41 2007 Subject: [Histonet] Re: Pregnancy References: <4761C6A6.2050207@conxis.com> Message-ID: <016801c83e4a$2ca17560$0302a8c0@yourxhtr8hvc4p> Laurie congratulations on everything. JTT ----- Original Message ----- From: "Laurie Popp" To: Sent: Thursday, December 13, 2007 5:56 PM Subject: [Histonet] Re: Pregnancy > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alinicoll <@t> yahoo.co.uk Fri Dec 14 06:37:43 2007 From: alinicoll <@t> yahoo.co.uk (ali nicoll) Date: Fri Dec 14 06:37:56 2007 Subject: [Histonet] Re: cassette labeling and slides labeling system (manufacturer response) Message-ID: <889370.43429.qm@web25609.mail.ukl.yahoo.com> Amy, May I point you in the direction of Raymond A Lamb who have new cassette and slide markers available either directly (in some countries) or via our distributors. The software package will also help with your requirements. Contact details can be found at www.ralamb.net. Regards Ali Nicoll [duck] ---------------------------- - Message: 3 Date: Wed, 12 Dec 2007 13:34:32 -0600 From: Jackie M O'Connor Subject: Re: [Histonet] cassette labeling and slides labeling system To: Amy Lee Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Leica has, in my opinion, the best system - one computer runs both printers, and can print cassettes and slides for the same case/study simultaneously. Amy Lee Sent by: histonet-bounces@lists.utsouthwestern.edu 12/12/2007 01:19 PM To histonet cc Subject [Histonet] cassette labeling and slides labeling system Hi histonetters, I am looking for a cassette labeling and slide labeling system. I know Shandon carry them. I am thinking having a package that both machines share same computer. Could any one give me any recommendation and any comment is highly appreciated. Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 49, Issue 14 **************************************** __________________________________________________________ Sent from Yahoo! Mail - a smarter inbox http://uk.mail.yahoo.com From CDuke <@t> Trianglebiomedical.com Fri Dec 14 08:19:34 2007 From: CDuke <@t> Trianglebiomedical.com (Cathy Duke) Date: Fri Dec 14 08:21:15 2007 Subject: [Histonet] Cassette labeling and Slide Labeling System (Manufacturer Response) Message-ID: <600E7CD630C81542ADCA1457EB6A8F4D02CF431D@tbs01.trianglebiomed.com> This message is for Amy Lee who was inquiring about Cassette and Slide Labeling Systems. Triangle Biomedical Sciences TBS has a new line of Cassette and Slide Labelers, please contact me at cduke@trianglebiomedical.com if interested in a demo. Thanks! Cathy-TBS From Shawna.Thomas <@t> Integris-Health.com Fri Dec 14 10:00:41 2007 From: Shawna.Thomas <@t> Integris-Health.com (Thomas, Shawna G.) Date: Fri Dec 14 10:01:11 2007 Subject: [Histonet] Re: Pregnancy In-Reply-To: <016801c83e4a$2ca17560$0302a8c0@yourxhtr8hvc4p> References: <4761C6A6.2050207@conxis.com> <016801c83e4a$2ca17560$0302a8c0@yourxhtr8hvc4p> Message-ID: <0AF4F8EA51200F49ADC32E6E353F024306EF6201@EXCHANGE2-OKC.corp.integris-health.com> When pregnant with my identical twin girls, I did gross dissection as usual. I did try to limit my exposure to xylene, but that was about it. I did not worry too much about the formalin exposure since my exposure limits have always been well below the acceptable levels. My girls were born at 35 weeks 5 days due to pre-eclampsia, but they were healthy and did not spend any time in the NICU. Now they are 22 months old and running all over the place. Shawna Baker, MBA, PA(ASCP) Pathologists' Assistant AmeriPath Oklahoma @ ISMC Pathology (405)644-6146 phone (405)636-7518 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, December 14, 2007 6:07 AM To: Laurie Popp; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Pregnancy Laurie congratulations on everything. JTT ----- Original Message ----- From: "Laurie Popp" To: Sent: Thursday, December 13, 2007 5:56 PM Subject: [Histonet] Re: Pregnancy > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain identifiable health information that is subject to protection under state and federal law. This information is intended to be for the use of the individual named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited and may be punishable by law. If you have received this electronic transmission in error, please notify us immediately by electronic mail (reply). From sae2001 <@t> med.cornell.edu Fri Dec 14 10:09:54 2007 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Fri Dec 14 10:10:15 2007 Subject: [Histonet] kappa and lambda BM ISH Message-ID: <7f72b225167a1.47626482@med.cornell.edu> Whereas our kappa and lambda ISH works well on LNs, it doesn't work on formalin fixed, EDTA-decalcified BMs, but other ISHs do (e.g. EBER1). Does anyone have a kappa and lambda ISH platform that works well on BMs? Would you please provide technical details? Thank you. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Thursday, December 13, 2007 1:16 pm Subject: Histonet Digest, Vol 49, Issue 14 From Samuel_Perry <@t> DFCI.HARVARD.EDU Fri Dec 14 10:25:33 2007 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Fri Dec 14 10:25:46 2007 Subject: [Histonet] Collagen I antibody recommendations? Message-ID: Hi All, I am looking for a nice Collagen I antibody to use on mouse and human tissues for IHC/DAB staining. I will be using the Dako envision kits for the staining. I was wondering if anyone has a Collagen I antibody they like? I found several possibilities on Biocompare. The one that looks the best so far is made by Abcam (cat. # ab34710). If anyone has used this antibody or recommends a different one I would like to hear more. Have a great Friday!! Thanks, Sam Perry Research Technician Dana Farber Cancer Institute Boston MA The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From liz <@t> premierlab.com Fri Dec 14 10:58:12 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Dec 14 10:58:25 2007 Subject: [Histonet] Collagen I antibody recommendations? In-Reply-To: <9D60C6EDAD37448A859DFE6F41FDDFDB@PremierLab.local> References: <9D60C6EDAD37448A859DFE6F41FDDFDB@PremierLab.local> Message-ID: Sam We use an antibody from santa cruz, its a goat anti-collagen I sc-25974 , works in both human and mouse, also goat, sheep, etc. I have a protocol if you need one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Samuel Sent: Friday, December 14, 2007 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Collagen I antibody recommendations? Hi All, I am looking for a nice Collagen I antibody to use on mouse and human tissues for IHC/DAB staining. I will be using the Dako envision kits for the staining. I was wondering if anyone has a Collagen I antibody they like? I found several possibilities on Biocompare. The one that looks the best so far is made by Abcam (cat. # ab34710). If anyone has used this antibody or recommends a different one I would like to hear more. Have a great Friday!! Thanks, Sam Perry Research Technician Dana Farber Cancer Institute Boston MA The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Fri Dec 14 13:03:25 2007 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Dec 14 13:03:40 2007 Subject: [Histonet] bone saws Message-ID: Hi, I'm looking for information on bone saws. It will primarily be used for femoral heads and knee caps. What kinds are you using? My Pathologist wants some kind of device that will immobilize the femoral heads so they don't roll around when cutting. Any ideas out there? As always your answers are appreciated! Stacy McLaughlin, HT Cooley Dickinson Hospital From Jason.Burrill <@t> crl.com Fri Dec 14 13:07:09 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Fri Dec 14 13:07:35 2007 Subject: [Histonet] RE: 10% formaldehyde?? Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E8F0ECE@shr-exch2.na01.crl.com> Dear Sara, According to the Dangerous Goods Regulations 10% formalin (4%formaldehyde) is not regulated by either the DOT or IATA.? The cut off for the percentage of formaldehyde in a solution that is considered a Dangerous Good and must be classified is ?10% formaldehyde.? So since 10% formalin is only 4% formaldehyde it is not regulated as a Dangerous Good but 10% formaldehyde is considered a "Aviation regulated liquid, n.o.s. UN3334" and subject to classification when shipping by air.? This is a common mistake for people who ship samples fixed in 10% formalin.? If you would like to discuss this further or would like additional references to confirm this please call or e-mail me. Regards, Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** From mpence <@t> grhs.net Fri Dec 14 13:18:54 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Dec 14 13:19:08 2007 Subject: [Histonet] bone saws In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C809@IS-E2K3.grhs.net> This is the saw we use for just that purpose. http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Friday, December 14, 2007 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone saws Hi, I'm looking for information on bone saws. It will primarily be used for femoral heads and knee caps. What kinds are you using? My Pathologist wants some kind of device that will immobilize the femoral heads so they don't roll around when cutting. Any ideas out there? As always your answers are appreciated! Stacy McLaughlin, HT Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Fri Dec 14 13:21:28 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Dec 14 13:25:03 2007 Subject: [Histonet] Re: Pregnancy References: <4761C6A6.2050207@conxis.com> Message-ID: My children are now 13,10, and almost 6 and my experience was the same. My last 2 children were higher risk because I was preeclamptic with the first and was diabetic with the last. I made a list in the lab of all the chemicals after I physically went through and read each MSDS. If anyone is interested I would happily fax it to you. Merry Christmas! Lynn Burton ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp Sent: Thu 12/13/2007 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Pregnancy Hi Histonetters, I actually just had a healthy baby girl 8/10/07 and was a high risk pregnancy ( diabetic) while finishing my HT and working full time in the histo lab at Mayo. I was advised by my OB to limit my formalin exposure, to limit my xylene exposure as much as possible, and to be very careful in special stains area. To also be careful of B-5 which we use for our bone marrows and the list goes on. Basically I was limited to cutting and embedding most days. Hope this helps! Laurie Popp, BA HT ( ASCP) and happy mommy to Kaiana :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schloesr <@t> mail.nih.gov Fri Dec 14 15:58:50 2007 From: schloesr <@t> mail.nih.gov (Robert Schloesser) Date: Fri Dec 14 15:59:03 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: Message-ID: Hi all Histotechs, We have a LEICA SM2000 R microtome. It worked fine for a few years but recently broke (the stage would not move anymore). We spend $2000 and over 3 month trying to get it repaired by LEICA. LEICAs customer service is completely incompetent and its technicians are even more incompetent (they came our more than 6 times - they even send someone from Chigaco here to DC to try to repair a simple microtome). Anyways, to make a sad story short I would STRONGLY advice everyone not to buy ANY products from LEICA that might require service. Furthermore, I would like to hear from you if there is a suggestion from the community what microtome to buy (since we have decided to throw our LEICA SM2000 in the trash). We need a microtome for cutting frozen brain tissue from rodents and primates. (usually 40um thick, sometimes up to 300um, sometimes thinner). We usually use STURKEY MICROTOME KNIFES. Any suggestions? Thank you Robert Robert J. Schloesser,MD visiting research fellow DHHS/NIH/NIMH/MAP/LMP Laboratory of Molecular Pathophysiology (LMP) Mood and Anxiety Disorders Program (MAP) National Institute of Mental Health (NIMH) Building 35/1C-912 35 Convent Drive Bethesda, MD 20892-3711 Phone: 301-451-8435 FAX: 301-480-0123 On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > My children are now 13,10, and almost 6 and my experience was the same. My > last 2 children were higher risk because I was preeclamptic with the first and > was diabetic with the last. I made a list in the lab of all the chemicals > after I physically went through and read each MSDS. If anyone is interested I > would happily fax it to you. > Merry Christmas! > Lynn Burton > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp > Sent: Thu 12/13/2007 5:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Pregnancy > > > > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jan.Minshew <@t> leica-microsystems.com Fri Dec 14 16:21:55 2007 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Fri Dec 14 16:22:05 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: Message-ID: Hello Dr. Schloesser, I am the Marketing Manager for the Leica SM2000 R and I would like to assure you that I was unaware of your difficulties. Please feel free to contact me so I can help resolve the obvious frustration you have encountered with the servicing of this instrument. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! Robert Schloesser To Sent by: histonet-bounces@ cc lists.utsouthwest service@leica-microsystems.com, ern.edu "Ernie.Oates@leica-microsystems.com " , PM Tino.Thakral@leica-microsystems.com Subject [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed Hi all Histotechs, We have a LEICA SM2000 R microtome. It worked fine for a few years but recently broke (the stage would not move anymore). We spend $2000 and over 3 month trying to get it repaired by LEICA. LEICAs customer service is completely incompetent and its technicians are even more incompetent (they came our more than 6 times - they even send someone from Chigaco here to DC to try to repair a simple microtome). Anyways, to make a sad story short I would STRONGLY advice everyone not to buy ANY products from LEICA that might require service. Furthermore, I would like to hear from you if there is a suggestion from the community what microtome to buy (since we have decided to throw our LEICA SM2000 in the trash). We need a microtome for cutting frozen brain tissue from rodents and primates. (usually 40um thick, sometimes up to 300um, sometimes thinner). We usually use STURKEY MICROTOME KNIFES. Any suggestions? Thank you Robert Robert J. Schloesser,MD visiting research fellow DHHS/NIH/NIMH/MAP/LMP Laboratory of Molecular Pathophysiology (LMP) Mood and Anxiety Disorders Program (MAP) National Institute of Mental Health (NIMH) Building 35/1C-912 35 Convent Drive Bethesda, MD 20892-3711 Phone: 301-451-8435 FAX: 301-480-0123 On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > My children are now 13,10, and almost 6 and my experience was the same. My > last 2 children were higher risk because I was preeclamptic with the first and > was diabetic with the last. I made a list in the lab of all the chemicals > after I physically went through and read each MSDS. If anyone is interested I > would happily fax it to you. > Merry Christmas! > Lynn Burton > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp > Sent: Thu 12/13/2007 5:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Pregnancy > > > > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cforster <@t> umn.edu Fri Dec 14 17:15:50 2007 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Dec 14 17:14:07 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: References: Message-ID: <47630EA6.30405@umn.edu> To Dr.Schloesser, Although I am sorry to hear that you have had trouble with your Leica rep. I have to say in their defense I have a very good one. They are always prompt to answer my phone calls and have always provided the service and support I need. I think it would have been better if you had contacted the main Leica company BEFORE you attempted to roast them on the histonet as Leica has a very good product and is a very reputable company. My 2 cents for the day!! Colleen Forster U of MN Jan.Minshew@leica-microsystems.com wrote: > Hello Dr. Schloesser, > > I am the Marketing Manager for the Leica SM2000 R and I would like to > assure you that I was unaware of your difficulties. Please feel free to > contact me so I can help resolve the obvious frustration you have > encountered with the servicing of this instrument. > > Best wishes, > > > Jan Minshew, HT/HTL(ASCP) > Marketing Manager > Leica Microsystems > Biosystems Division > 2345 Waukegan Road > Bannockburn, IL 60015 > > 800.248.0123 Toll Free > 847.405.7051 Direct > 847.405.6560 Fax > > www.leica-microsystems.com > > Click Here for this month's special offers! > > > > Robert Schloesser > h.gov> To > Sent by: > histonet-bounces@ cc > lists.utsouthwest service@leica-microsystems.com, > ern.edu "Ernie.Oates@leica-microsystems.com > " > 12/14/2007 03:58 >, > PM Tino.Thakral@leica-microsystems.com > Subject > [Histonet] WARNING ABOUT LEICA > MICROTOMES AND SERVICE and > Suggestions needed > > > > > > > > > > > Hi all Histotechs, > > We have a LEICA SM2000 R microtome. It worked fine for a few years but > recently broke (the stage would not move anymore). We spend $2000 and over > 3 > month trying to get it repaired by LEICA. LEICAs customer service is > completely incompetent and its technicians are even more incompetent (they > came our more than 6 times - they even send someone from Chigaco here to DC > to try to repair a simple microtome). > > Anyways, to make a sad story short I would STRONGLY advice everyone not to > buy ANY products from LEICA that might require service. > > Furthermore, I would like to hear from you if there is a suggestion from > the > community what microtome to buy (since we have decided to throw our LEICA > SM2000 in the trash). We need a microtome for cutting frozen brain tissue > from rodents and primates. (usually 40um thick, sometimes up to 300um, > sometimes thinner). We usually use STURKEY MICROTOME KNIFES. > > Any suggestions? Thank you > > Robert > > > Robert J. Schloesser,MD > visiting research fellow > DHHS/NIH/NIMH/MAP/LMP > > Laboratory of Molecular Pathophysiology (LMP) > Mood and Anxiety Disorders Program (MAP) > National Institute of Mental Health (NIMH) > > Building 35/1C-912 > 35 Convent Drive > Bethesda, MD 20892-3711 > Phone: 301-451-8435 > FAX: 301-480-0123 > > > > > On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > > >> My children are now 13,10, and almost 6 and my experience was the same. >> > My > >> last 2 children were higher risk because I was preeclamptic with the >> > first and > >> was diabetic with the last. I made a list in the lab of all the chemicals >> after I physically went through and read each MSDS. If anyone is >> > interested I > >> would happily fax it to you. >> Merry Christmas! >> Lynn Burton >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp >> Sent: Thu 12/13/2007 5:56 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Pregnancy >> >> >> >> Hi Histonetters, >> >> I actually just had a healthy baby girl 8/10/07 and was a high risk >> pregnancy ( diabetic) while finishing my HT and working full time in the >> histo lab at Mayo. I was advised by my OB to limit my formalin >> exposure, to limit my xylene exposure as much as possible, and to be >> very careful in special stains area. To also be careful of B-5 which we >> use for our bone marrows and the list goes on. Basically I was limited >> to cutting and embedding most days. >> >> Hope this helps! >> >> Laurie Popp, BA HT ( ASCP) >> and happy mommy to Kaiana :-) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gayle.callis <@t> bresnan.net Fri Dec 14 18:20:45 2007 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Dec 14 18:20:53 2007 Subject: [Histonet] Leica microtomes, service and suggestion Message-ID: <001301c83eb0$56276e10$6501a8c0@DHXTS541> TO: Robert J. Schloesser, MD Visiting Research Fellow Our experience with Leica has been one of extreme satisfaction for both longevity of equipment and service for all our Leica microtomes and cryostats over the past 27 years. In fact their service has been above and beyond the call of duty more times than once. They have bent over backward to make things work for us, and will for you too, if you contact the company directly. It is considered impolite and rather unprofessional to air personal complaints or bash any company/vendor on Histonet. However you did request a suggestion for an instrument to cryosection brain. I would highly recommend what Leica has to offer as their equipment is excellent. Either a cryostat, microtome to handle frozen brain or working with their vibrating microtome for fresh and/or fixed tissues. Always a satisfied Leica customer for microtomes AND cryostats Gayle Callis HTL,HT,MT(ASCP) Bozeman MT From hodges420 <@t> msn.com Fri Dec 14 18:24:33 2007 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Dec 14 18:24:47 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: References: Message-ID: we have had great service from our rep Mary and service guys Bret and Ken... maybe you should have called your rep I can't give them a higher rating best we have even had Tere Hodges Tucson,Az> To: schloesr@mail.nih.gov> From: Jan.Minshew@leica-microsystems.com> Date: Fri, 14 Dec 2007 16:21:55 -0600> Subject: Re: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed> CC: histonet@lists.utsouthwestern.edu; service@leica-microsystems.com; histonet-bounces@lists.utsouthwestern.edu; Ernie.Oates@leica-microsystems.com; Tino.Thakral@leica-microsystems.com> > Hello Dr. Schloesser,> > I am the Marketing Manager for the Leica SM2000 R and I would like to> assure you that I was unaware of your difficulties. Please feel free to> contact me so I can help resolve the obvious frustration you have> encountered with the servicing of this instrument.> > Best wishes,> > > Jan Minshew, HT/HTL(ASCP)> Marketing Manager> Leica Microsystems> Biosystems Division> 2345 Waukegan Road> Bannockburn, IL 60015> > 800.248.0123 Toll Free> 847.405.7051 Direct> 847.405.6560 Fax> > www.leica-microsystems.com> > Click Here for this month's special offers!> > > > Robert Schloesser > h.gov> To > Sent by: > histonet-bounces@ cc > lists.utsouthwest service@leica-microsystems.com, > ern.edu "Ernie.Oates@leica-microsystems.com > " > 12/14/2007 03:58 >, > PM Tino.Thakral@leica-microsystems.com > Subject > [Histonet] WARNING ABOUT LEICA > MICROTOMES AND SERVICE and > Suggestions needed > > > > > > > > > > > Hi all Histotechs,> > We have a LEICA SM2000 R microtome. It worked fine for a few years but> recently broke (the stage would not move anymore). We spend $2000 and over> 3> month trying to get it repaired by LEICA. LEICAs customer service is> completely incompetent and its technicians are even more incompetent (they> came our more than 6 times - they even send someone from Chigaco here to DC> to try to repair a simple microtome).> > Anyways, to make a sad story short I would STRONGLY advice everyone not to> buy ANY products from LEICA that might require service.> > Furthermore, I would like to hear from you if there is a suggestion from> the> community what microtome to buy (since we have decided to throw our LEICA> SM2000 in the trash). We need a microtome for cutting frozen brain tissue> from rodents and primates. (usually 40um thick, sometimes up to 300um,> sometimes thinner). We usually use STURKEY MICROTOME KNIFES.> > Any suggestions? Thank you> > Robert> > > Robert J. Schloesser,MD> visiting research fellow> DHHS/NIH/NIMH/MAP/LMP> > Laboratory of Molecular Pathophysiology (LMP)> Mood and Anxiety Disorders Program (MAP)> National Institute of Mental Health (NIMH)> > Building 35/1C-912> 35 Convent Drive> Bethesda, MD 20892-3711> Phone: 301-451-8435> FAX: 301-480-0123> > > > > On 12/14/07 2:21 PM, "Burton, Lynn" wrote:> > > My children are now 13,10, and almost 6 and my experience was the same.> My> > last 2 children were higher risk because I was preeclamptic with the> first and> > was diabetic with the last. I made a list in the lab of all the chemicals> > after I physically went through and read each MSDS. If anyone is> interested I> > would happily fax it to you.> > Merry Christmas!> > Lynn Burton> >> > ________________________________> >> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp> > Sent: Thu 12/13/2007 5:56 PM> > To: histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Re: Pregnancy> >> >> >> > Hi Histonetters,> >> > I actually just had a healthy baby girl 8/10/07 and was a high risk> > pregnancy ( diabetic) while finishing my HT and working full time in the> > histo lab at Mayo. I was advised by my OB to limit my formalin> > exposure, to limit my xylene exposure as much as possible, and to be> > very careful in special stains area. To also be careful of B-5 which we> > use for our bone marrows and the list goes on. Basically I was limited> > to cutting and embedding most days.> >> > Hope this helps!> >> > Laurie Popp, BA HT ( ASCP)> > and happy mommy to Kaiana :-)> >> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ______________________________________________________________________> This email has been scanned by the MessageLabs Email Security System.> For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The best games are on Xbox 360. Click here for a special offer on an Xbox 360 Console. http://www.xbox.com/en-US/hardware/wheretobuy/ From gu.lang <@t> gmx.at Sat Dec 15 06:46:34 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Dec 15 06:46:52 2007 Subject: AW: [Histonet] kappa and lambda BM ISH In-Reply-To: <7f72b225167a1.47626482@med.cornell.edu> Message-ID: <000801c83f18$86b430a0$0202fea9@dielangs.at> I know this is no help, but we also had these problems on demonstrating kappa and lambda per ISH, and quit it. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Scott A. Ely Gesendet: Freitag, 14. Dezember 2007 17:10 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] kappa and lambda BM ISH Whereas our kappa and lambda ISH works well on LNs, it doesn't work on formalin fixed, EDTA-decalcified BMs, but other ISHs do (e.g. EBER1). Does anyone have a kappa and lambda ISH platform that works well on BMs? Would you please provide technical details? Thank you. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Thursday, December 13, 2007 1:16 pm Subject: Histonet Digest, Vol 49, Issue 14 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Sat Dec 15 08:12:12 2007 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Sat Dec 15 08:12:28 2007 Subject: [Histonet] Leica microtomes, service and suggestion In-Reply-To: <001301c83eb0$56276e10$6501a8c0@DHXTS541> References: <001301c83eb0$56276e10$6501a8c0@DHXTS541> Message-ID: <1197727932.4763e0bcc598f@imp.vet.upenn.edu> I agree with Gayle and all who are satisfied with Leica equipment and service. In fact my only complaint would be it is so popular it is sometimes hard to get a piece of equipment as fast as you may want it. Service has been excellent and I can always reach someone to get help. Personal issues should be handled in private and not over HiatoNet. We would all have been happy to make suggestions for microtomes if that had been your question. It may have been a problem that so many of us recommended Leica. I have a research cryostat and three microtomes so we have a history for paraffin, plastics and great frozens with Leica and would not consider any others to replace or add to our laboratory. Pam Marcum Quoting Gayle Callis : > TO: Robert J. Schloesser, MD > Visiting Research Fellow > > Our experience with Leica has been one of extreme satisfaction for both > longevity of equipment and service for all our Leica microtomes and cryostats > over the past 27 years. In fact their service has been above and beyond the > call of duty more times than once. They have bent over backward to make > things work for us, and will for you too, if you contact the company > directly. It is considered impolite and rather unprofessional to air > personal complaints or bash any company/vendor on Histonet. > > However you did request a suggestion for an instrument to cryosection brain. > I would highly recommend what Leica has to offer as their equipment is > excellent. Either a cryostat, microtome to handle frozen brain or working > with their vibrating microtome for fresh and/or fixed tissues. > > Always a satisfied Leica customer for microtomes AND cryostats > > Gayle Callis > HTL,HT,MT(ASCP) > Bozeman MT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ccrowder <@t> vetmed.lsu.edu Sat Dec 15 09:04:26 2007 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sat Dec 15 09:09:34 2007 Subject: [Histonet] Immunofluorescence Message-ID: Hello - I have a researcher who has done IHC on her tissues with good success. Now she is trying to do fluorescent antibodies on thick sections to be able to do 3D imaging. I have no experience with this technique. Her problem, tremendous background staining. Can anyone give us any tips for reducing this staining? Thanking you in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From rjbuesa <@t> yahoo.com Sat Dec 15 09:39:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 15 09:39:39 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: Message-ID: <749281.81657.qm@web61216.mail.yahoo.com> Dr. Schloesser: I read with disbelief your complaint about Leica microtomes (centered on one particular model). The fact that you had difficulties with a particular rep. does not warrant your "call for boicoting" these extremely good instruments. They have been excellent since the XIX century, and keep being the best in the XXI century. I find unfortunate your comments and I think your should have thought them through before posting them, they are inaccurate and help nobody. Ren? J. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From ratliffjack <@t> hotmail.com Sat Dec 15 09:49:01 2007 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Sat Dec 15 09:49:15 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: References: Message-ID: I am compelled to reply to this disturbing message as I STRONGLY feel very disturbed that ANYONE would actually use this forum to send such a dirty and misleading message for the eyes of thousands of histonet readers! I have USED, PURCHASED, and RECOMMENDED the purchase/use of LEICA equipment ever since I entered the field 11 years ago. In fact, I continue to purchase, use, and recommend the use of this equipment to this very day and I have NEVER had such an issue as described in the original message! Additionally, I support this equipment because of the many, many positive experiences that I have had with both the equipment and the representatives over these years! I cannot even fathom the problems as suggested as it is very true that the equipment is well engineered. Lastly, in all my dealings with LEICA representatives over the years, I NEVER could imagine that the service would be as the original message has described. In my experience, these representatives are true professionals, unlike the authors comments, tactics, and/or motives in the original message on this subject. Jack Ratliff PS If your microtome has not left for the dump yet, I would LOVE to take it off your hands! > Date: Fri, 14 Dec 2007 16:58:50 -0500> From: schloesr@mail.nih.gov> To: histonet@lists.utsouthwestern.edu> CC: service@leica-microsystems.com; Ernie.Oates@leica-microsystems.com; Tino.Thakral@leica-microsystems.com> Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed> > Hi all Histotechs, > > We have a LEICA SM2000 R microtome. It worked fine for a few years but> recently broke (the stage would not move anymore). We spend $2000 and over 3> month trying to get it repaired by LEICA. LEICAs customer service is> completely incompetent and its technicians are even more incompetent (they> came our more than 6 times - they even send someone from Chigaco here to DC> to try to repair a simple microtome).> > Anyways, to make a sad story short I would STRONGLY advice everyone not to> buy ANY products from LEICA that might require service.> > Furthermore, I would like to hear from you if there is a suggestion from the> community what microtome to buy (since we have decided to throw our LEICA> SM2000 in the trash). We need a microtome for cutting frozen brain tissue> from rodents and primates. (usually 40um thick, sometimes up to 300um,> sometimes thinner). We usually use STURKEY MICROTOME KNIFES.> > Any suggestions? Thank you> > Robert> > > Robert J. Schloesser,MD> visiting research fellow> DHHS/NIH/NIMH/MAP/LMP> > Laboratory of Molecular Pathophysiology (LMP)> Mood and Anxiety Disorders Program (MAP)> National Institute of Mental Health (NIMH)> > Building 35/1C-912> 35 Convent Drive> Bethesda, MD 20892-3711> Phone: 301-451-8435> FAX: 301-480-0123> > > > > On 12/14/07 2:21 PM, "Burton, Lynn" wrote:> > > My children are now 13,10, and almost 6 and my experience was the same. My> > last 2 children were higher risk because I was preeclamptic with the first and> > was diabetic with the last. I made a list in the lab of all the chemicals> > after I physically went through and read each MSDS. If anyone is interested I> > would happily fax it to you.> > Merry Christmas!> > Lynn Burton> > > > ________________________________> > > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp> > Sent: Thu 12/13/2007 5:56 PM> > To: histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Re: Pregnancy> > > > > > > > Hi Histonetters,> > > > I actually just had a healthy baby girl 8/10/07 and was a high risk> > pregnancy ( diabetic) while finishing my HT and working full time in the> > histo lab at Mayo. I was advised by my OB to limit my formalin> > exposure, to limit my xylene exposure as much as possible, and to be> > very careful in special stains area. To also be careful of B-5 which we> > use for our bone marrows and the list goes on. Basically I was limited> > to cutting and embedding most days.> > > > Hope this helps!> > > > Laurie Popp, BA HT ( ASCP)> > and happy mommy to Kaiana :-)> > > > > > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The best games are on Xbox 360. Click here for a special offer on an Xbox 360 Console. http://www.xbox.com/en-US/hardware/wheretobuy/ From rjbuesa <@t> yahoo.com Sat Dec 15 09:54:13 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 15 09:54:24 2007 Subject: [Histonet] Immunofluorescence In-Reply-To: Message-ID: <876147.20951.qm@web61218.mail.yahoo.com> No matter what you do, with thick sections you will always get background noise. Try to use thinner sections. Ren? J. Cheryl Crowder wrote: Hello - I have a researcher who has done IHC on her tissues with good success. Now she is trying to do fluorescent antibodies on thick sections to be able to do 3D imaging. I have no experience with this technique. Her problem, tremendous background staining. Can anyone give us any tips for reducing this staining? Thanking you in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From schloesr <@t> mail.nih.gov Sat Dec 15 10:24:53 2007 From: schloesr <@t> mail.nih.gov (Robert Schloesser) Date: Sat Dec 15 10:25:29 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: Message-ID: Dear all, I would like to use this email to APOLOGIZE for, but also explain the motives for my earlier email warning about LEICA service in particular our experience with our LEICA SM200R microtome. First of all, I would like to apologize because I do agree that the wording of this email was not appropriate for this forum. The reason for the email was an extreme level of frustration with our Leica service technicians here at NIH: The group of techs servicing our LEICA SM2000R were unable to repair the microtome for over 9 weeks and it is still not functional. We paid $1600 for ?urgent? service, but even after 7 repair trials the mechanism that moves the stage keeps on failing after little use or (yesterday) the handle fell off. This has caused us a tremendous loss of valuable time as well as money hence my frustration induced overreaction. The many hours we spend talking to various levels of LEICA service hotlines did not lead to any appropriate reaction and the communication with the manager of our service technician team was very unproductive and at times unpleasant, again leading to a buildup of frustration that sparked in my inappropriate misuse of this forum. Please note 1. I agree that ?when functional? the LEICA SM2000R is a very convenient to use microtome (I have used it for over 3 years) 2. LEICA SM2000R marketing representatives called and emailed me less than 15 minutes after my original email and initiated a phone conference with the technical service headquarter. LEICA reviewed our case within minutes and confirmed that it was an unusual case of unusually bad service. I would like to use this email to thank LEICAs headquarter for this quick response and express my hope and optimism that the promised changes will be made at the our service department and we will not have to wait for another 9 weeks before our microtome starts working again. 3. Again, I agree that my previous email was not appropriately worded since it was written out of frustration. I should have given this more time for thought. Please accept these apologies since I highly value this forum and its community Robert On 12/15/07 10:49 AM, "Jack Ratliff" wrote: > I am compelled to reply to this disturbing message as I STRONGLY feel very > disturbed that ANYONE would actually use this forum to send such a dirty and > misleading message for the eyes of thousands of histonet readers! > > I have USED, PURCHASED, and RECOMMENDED the purchase/use of LEICA equipment > ever since I entered the field 11 years ago. In fact, I continue to purchase, > use, and recommend the use of this equipment to this very day and I have NEVER > had such an issue as described in the original message! Additionally, I > support this equipment because of the many, many positive experiences that I > have had with both the equipment and the representatives over these years! > > I cannot even fathom the problems as suggested as it is very true that the > equipment is well engineered. Lastly, in all my dealings with LEICA > representatives over the years, I NEVER could imagine that the service would > be as the original message has described. In my experience, these > representatives are true professionals, unlike the authors comments, tactics, > and/or motives in the original message on this subject. > > Jack Ratliff > > PS If your microtome has not left for the dump yet, I would LOVE to take it > off your hands! > > > > > > > > > > >> > Date: Fri, 14 Dec 2007 16:58:50 -0500 >> > From: schloesr@mail.nih.gov >> > To: histonet@lists.utsouthwestern.edu >> > CC: service@leica-microsystems.com; Ernie.Oates@leica-microsystems.com; >> Tino.Thakral@leica-microsystems.com >> > Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and >> Suggestions needed >> > >> > Hi all Histotechs, >> > >> > We have a LEICA SM2000 R microtome. It worked fine for a few years but >> > recently broke (the stage would not move anymore). We spend $2000 and over 3 >> > month trying to get it repaired by LEICA. LEICAs customer service is >> > completely incompetent and its technicians are even more incompetent (they >> > came our more than 6 times - they even send someone from Chigaco here to DC >> > to try to repair a simple microtome). >> > >> > Anyways, to make a sad story short I would STRONGLY advice everyone not to >> > buy ANY products from LEICA that might require service. >> > >> > Furthermore, I would like to hear from you if there is a suggestion from >> the >> > community what microtome to buy (since we have decided to throw our LEICA >> > SM2000 in the trash). We need a microtome for cutting frozen brain tissue >> > from rodents and primates. (usually 40um thick, sometimes up to 300um, >> > sometimes thinner). We usually use STURKEY MICROTOME KNIFES. >> > >> > Any suggestions? Thank you >> > >> > Robert >> > >> > >> > Robert J. Schloesser,MD >> > visiting research fellow >> > DHHS/NIH/NIMH/MAP/LMP >> > >> > Laboratory of Molecular Pathophysiology (LMP) >> > Mood and Anxiety Disorders Program (MAP) >> > National Institute of Mental Health (NIMH) >> > >> > Building 35/1C-912 >> > 35 Convent Drive >> > Bethesda, MD 20892-3711 >> > Phone: 301-451-8435 >> > FAX: 301-480-0123 >> > >> > >> > >> > >> > On 12/14/07 2:21 PM, "Burton, Lynn" wrote: >> > >>> > > My children are now 13,10, and almost 6 and my experience was the same. My >>> > > last 2 children were higher risk because I was preeclamptic with the >>> first and >>> > > was diabetic with the last. I made a list in the lab of all the >>> chemicals >>> > > after I physically went through and read each MSDS. If anyone is >>> interested I >>> > > would happily fax it to you. >>> > > Merry Christmas! >>> > > Lynn Burton >>> > > >>> > > ________________________________ >>> > > >>> > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp >>> > > Sent: Thu 12/13/2007 5:56 PM >>> > > To: histonet@lists.utsouthwestern.edu >>> > > Subject: [Histonet] Re: Pregnancy >>> > > >>> > > >>> > > >>> > > Hi Histonetters, >>> > > >>> > > I actually just had a healthy baby girl 8/10/07 and was a high risk >>> > > pregnancy ( diabetic) while finishing my HT and working full time in the >>> > > histo lab at Mayo. I was advised by my OB to limit my formalin >>> > > exposure, to limit my xylene exposure as much as possible, and to be >>> > > very careful in special stains area. To also be careful of B-5 which we >>> > > use for our bone marrows and the list goes on. Basically I was limited >>> > > to cutting and embedding most days. >>> > > >>> > > Hope this helps! >>> > > >>> > > Laurie Popp, BA HT ( ASCP) >>> > > and happy mommy to Kaiana :-) >>> > > >>> > > >>> > > _______________________________________________ >>> > > Histonet mailing list >>> > > Histonet@lists.utsouthwestern.edu >>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > > >>> > > >>> > > _______________________________________________ >>> > > Histonet mailing list >>> > > Histonet@lists.utsouthwestern.edu >>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > The best games are on Xbox 360. Click here for a special offer on an Xbox 360 > Console. Get it now! From koellingr <@t> comcast.net Sat Dec 15 10:56:00 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Dec 15 10:56:17 2007 Subject: [Histonet] Immunofluorescence Message-ID: <121520071656.21647.47640720000CCA110000548F22070215739D09020704040A0105@comcast.net> Cheryl, What helped me previously is using a confocal flourescent scope and I'm assuming at LSU there are many. Looking through a "thick section" with traditional flourescence, you will always be burdened with increased background since you are imaging through the entire section. With confocal, all that total background becomes irrelavent since , depending on the power of your confocal, you can "optically" section through the thick section, a few micron optical slices at a time. And 3D image the results to boot by stacking them depending on the software you have. Raymond Koelling PhenPath Labs Seattle, WA -------------- Original message -------------- From: Rene J Buesa > No matter what you do, with thick sections you will always get background noise. > Try to use thinner sections. > Ren$B!&(BJ. > > Cheryl Crowder wrote: > Hello - I have a researcher who has done IHC on her tissues with good > success. Now she is trying to do fluorescent antibodies on thick sections > to be able to do 3D imaging. I have no experience with this technique. Her > problem, tremendous background staining. Can anyone give us any tips for > reducing this staining? Thanking you in advance, > Cheryl > Cheryl Crowder, BA, HTL(ASCP) > > Chief Technologist > > Anatomic Pathology > > Department of Pathobiological Sciences > > School of Veterinary Medicine > > Louisiana State University > > Skip Bertman Drive > > Baton Rouge, LA 70803 > > > > 225-578-9734 > > FAX: 225-578-9720 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sat Dec 15 15:17:10 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sat Dec 15 15:17:28 2007 Subject: [Histonet] Re: bone saws Message-ID: Mike Pence recommends this bone saw: This is the saw we use for just that purpose. http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html This or a very similar device is called a "Saw Bones" and consists of a clamp and two parallel hacksaws that cut a slab of bone of the right thickness for histologic examination. I've used such a saw and recommend it highly. The only drawback is that since it costs around $500 and is something the pathologist actually has his hands on, the answer from management is usually no. Pathology service policies vary widely on how femoral heads and specimens from total knee replacements are processed. Some hospitals allow the orthopedist to throw them away. In my experience these specimens need a gross examination to verify the arthritic changes for the high school dropout who determines whether the orthopedist gets paid or not (this really happened to an orthopedist of my acquaintance). My standard sign-out is head of left femur (total joint replacement): eburnation and osteophyte formation, consistent with end stage osteoarthritis. I insist on decalcified sections of hip fracture specimens, since unexpected metastatic cancer with "pathologic fracture" is something you see every now and then (actually, I've seen it three times in my life). Osteoarthritis specimens are much denser and more difficult to saw, and the yield of significant observations is lower, but doing microscopy on these specimens is a revenue producer for the pathologist and/or the hospital, by upping the CPT code from 88300 to 88305 and 88311. (Don't expect to cost-justify the Saw Bones with this argument though - they teach you in M.B.A. school not to think like that). (Now I'll get flamed, not by Leica, but by the likes of Harvard Business School.) Bob Richmond Samurai Pathologist Knoxville TN From lpwenk <@t> sbcglobal.net Sat Dec 15 15:47:07 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Dec 15 15:47:31 2007 Subject: [Histonet] prolonged impregnation in Golgi-Cox (Kolb) staining In-Reply-To: Message-ID: <000301c83f64$0a142130$0202a8c0@HPPav2> I don't know if the tissue can be "saved", but try washing in water for a few hours then process as usual for this technique, and see what happens A lot of things are working against you. The dichromate has been oxidized, the mercuric chloride has released a lot of hydrochloric acid which has been chewing up the proteins. And, boy, do you have a lot of fixative cross-links from both the chomate and the mercuric that you normally wouldn't have. As for the green color, I think that's due to the normally orangish/red potassium dichromate Cr+6 being oxidized to green Cr+3 in an acidic environment. The oxidizer is the air in the container, and the acidic environment comes from hydrochloric acid being released from the mercuric chloride during cross-linking with the tissue. I have a couple of questions, for anyone in the Histonet community. This Golgi-Cox fixation procedure comes up a on occasion on Histonet, mostly from researchers. How do these labs dispose of the chemicals afterwards? Mercuric chloride cannot be dumped down any sink, and most (but not all) water/sewer treatment plants won't allow potassium dichromate to be disposed down the sink either. And how do researchers dispose of the water and alcohol and xylene in the processing afterwards, where mercuric chloride and potassium dichromate are being pulled out of the tissue into the processing solutions? Can't these tissues be fixed in formalin (or some other less toxic fixative than mercury and chromium), and then IHC procedures done, such as antibodies against GFAP or neurofilaments or NSE? I work in a hospital, and am just wondering why this Golgi-Cox procedure is needed by researchers, but doesn't seem to be needed by clinical histology laboratories. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Olek Michalski Sent: Thursday, December 13, 2007 3:31 PM To: Histonet Subject: [Histonet] prolonged impregnation in Golgi-Cox (Kolb) staining Dear Histonetters, I have some brains which were left in Golgi-Cox chromation solution (K2Cr2O7, KCrO3, HgCl2: about 1% of each) for over 6 months. I would really like to use this material even if staining would not be very clear. Do you have an idea how to process the tissue in this case? Any help would be appreciated (and I know, next time I will be more careful about where I leave my preparates). By the way, I have already cut one of these brains and I found the sections to be dark green. I have noticed this colour in my G-C preparates before but mainly on the surface. I am actually curious whether the tissue should be more green or more red when I cut it. I mean should I shorten the normal period of chromation (about 14-15 days) if I see the brain is getting green? Yours truly Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Sat Dec 15 16:33:57 2007 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Dec 15 16:34:12 2007 Subject: [Histonet] Immunofluorescence References: Message-ID: <002701c83f6a$94ec0c40$6501a8c0@DHXTS541> Cheryl, You didn't say how thick the sections will be? Go to www.IHCworld.org and click on the fluorescence area, then autofluorescence and how tissues sections can be treated to reduce this problem. One thing that can be done is USE the autofluorescence as a counterfluorescence i.e. like a counterstain. If the tissue is prefixed with NBF or paraformaldehyde, then aldehyde induced autofluorescence results. If she uses a fluorophore that is the opposite color of the autofluorescence, say that is greenish yellow, then a fluorophore that is red works much better. Also a fluorophore that is near infrared region helps too, although one cannot visualize this with the human eye. Be sure the fluorophore is the brightest possible, and resistant to photobleaching. Recommended is Molecular Probes/Invitrogen Alexa 594 (an equivalent to Texas Red). One can conjugate your own antibody or buy secondaries conjugated to this flourophore. There are chemical treatments to reduce autofluorescence on aldehyde fixed tissues, even after fixation and before sectioning or on the section itself. One method uses 100 mM glycine treatment and this has been discussed on Histonet, check out the archives. If the tissue is vibratomed in fresh state, then using a red fluorophore conjugated antibody can give some spectacular results. One lab here vibratomes fresh lung tissue (approx 100 um thick sections) containing cells labeled with Texas Red antibody. The fresh tissue autofluoresced green, cells red, and they did CLSM z stacks with great success using an inverted microcope with a Zeiss CLSM. If one can avoid aldehyde fixation, that helps but that is not always the case. There is also a confocal listserver out of Buffalo, similar to Histonet that can be invaluable for more specific information when using the confocal for z stack imaging. Have her subscribe to that and also get into their archives. An excellent listserver. Good luck Gayle Callis HT.,HT,MT(ASCP) ----- Original Message ----- From: "Cheryl Crowder" To: Sent: Saturday, December 15, 2007 8:04 AM Subject: [Histonet] Immunofluorescence > Hello - I have a researcher who has done IHC on her tissues with good > success. Now she is trying to do fluorescent antibodies on thick sections > to be able to do 3D imaging. I have no experience with this technique. > Her > problem, tremendous background staining. Can anyone give us any tips for > reducing this staining? Thanking you in advance, > Cheryl > Cheryl Crowder, BA, HTL(ASCP) > > Chief Technologist > > Anatomic Pathology > > Department of Pathobiological Sciences > > School of Veterinary Medicine > > Louisiana State University > > Skip Bertman Drive > > Baton Rouge, LA 70803 > > > > 225-578-9734 > > FAX: 225-578-9720 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pinetree110567 <@t> yahoo.com Sat Dec 15 17:08:34 2007 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Sat Dec 15 17:08:47 2007 Subject: [Histonet] Cassette and slide labeling Message-ID: <629766.50070.qm@web44916.mail.sp1.yahoo.com> I prefer the ThermoFisher system for many reasons. 1. The Thermo units can be run from one PC. 2. Thermo does not use ink, the Leica unit when it prints it prints a faint grey that looks ok at best only on white cassettes and if you use certain lab chemicals the ink comes off. Also, the Leica costs a fortune to run, just like your home printer the printer is cheap... they usually give you the printer to get you buying ink and when you do the ink costs you an arm and a leg. I have run a report and have printed over 15,000 cassettes on my Thermo unit for 22 dollars of foil, while the same amount would cost over $2,000 on a Leica printer, I bet Leica didn't tell you that, they didnn't tell me when I bought my unit. Also, multiply that by two for both the cassette writer and slide writer!!! 3. Thermo is selling their units through RA Lamb and TBS, this gives you more outlets for parts and cassettes and it has a much smaller foot print. I don't want to sound as if I am bashing Leica, I have two microtomes that I would not trade for any other brand, it is just I feel misled concerning the cost and performance of their writers. ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From kemlo <@t> f2s.com Sun Dec 16 04:23:21 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sun Dec 16 04:23:45 2007 Subject: [Histonet] Leica microtomes, service and suggestion In-Reply-To: <1197727932.4763e0bcc598f@imp.vet.upenn.edu> References: <001301c83eb0$56276e10$6501a8c0@DHXTS541> <1197727932.4763e0bcc598f@imp.vet.upenn.edu> Message-ID: <526C7C2CE5C54F4BA0EE76F91F3A378B@KemloPC> That this Forum is used to criticise Suppliers is surely one of its functions? The response from Leica appears professional and hopefully the issue will be resolved and this Forum will have served its purpose. As a Brit I'm surprised that the US's legal system didn't kick in (or the UKs impression of that legal system), but if you can't trash someone's kit here, them where else? Surely the good Doctor's frustrations have now been aired and Leica will give him a Chrissy present of a new 'tome. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pmarcum@vet.upenn.edu Sent: 15 December 2007 14:12 To: Gayle Callis Cc: Histonet Subject: Re: [Histonet] Leica microtomes, service and suggestion I agree with Gayle and all who are satisfied with Leica equipment and service. In fact my only complaint would be it is so popular it is sometimes hard to get a piece of equipment as fast as you may want it. Service has been excellent and I can always reach someone to get help. Personal issues should be handled in private and not over HiatoNet. We would all have been happy to make suggestions for microtomes if that had been your question. It may have been a problem that so many of us recommended Leica. I have a research cryostat and three microtomes so we have a history for paraffin, plastics and great frozens with Leica and would not consider any others to replace or add to our laboratory. Pam Marcum Quoting Gayle Callis : > TO: Robert J. Schloesser, MD > Visiting Research Fellow > > Our experience with Leica has been one of extreme satisfaction for both > longevity of equipment and service for all our Leica microtomes and cryostats > over the past 27 years. In fact their service has been above and beyond the > call of duty more times than once. They have bent over backward to make > things work for us, and will for you too, if you contact the company > directly. It is considered impolite and rather unprofessional to air > personal complaints or bash any company/vendor on Histonet. > > However you did request a suggestion for an instrument to cryosection brain. > I would highly recommend what Leica has to offer as their equipment is > excellent. Either a cryostat, microtome to handle frozen brain or working > with their vibrating microtome for fresh and/or fixed tissues. > > Always a satisfied Leica customer for microtomes AND cryostats > > Gayle Callis > HTL,HT,MT(ASCP) > Bozeman MT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billingconsultants <@t> yahoo.com Sun Dec 16 06:13:21 2007 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Sun Dec 16 06:13:35 2007 Subject: [Histonet] LEICA Message-ID: <592178.55297.qm@web54205.mail.re2.yahoo.com> I have nothing but the highest regard for Leica sales, equipment and service. They have some of the most dependable equipment on the market and are second to none in reponsiveness. To advise people not to buy their products before contacting the main company is wrong. Louri Roberts Director Client Services Billing Consultants, LLC www.billingconsultants.net histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. bone saws (Stacy McLaughlin) 2. RE: 10% formaldehyde?? (Burrill, Jason) 3. RE: bone saws (Mike Pence) 4. RE: Re: Pregnancy (Burton, Lynn) 5. WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (Robert Schloesser) 6. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (Jan.Minshew@leica-microsystems.com) 7. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (Colleen Forster) 8. Leica microtomes, service and suggestion (Gayle Callis) 9. RE: WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (MARY T HODGES) 10. AW: [Histonet] kappa and lambda BM ISH (Gudrun Lang) 11. Re: Leica microtomes, service and suggestion (pmarcum@vet.upenn.edu) 12. Immunofluorescence (Cheryl Crowder) 13. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (Rene J Buesa) 14. RE: WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (Jack Ratliff) 15. Re: Immunofluorescence (Rene J Buesa) 16. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed (Robert Schloesser) 17. Re: Immunofluorescence (koellingr@comcast.net) ---------------------------------------------------------------------- Message: 1 Date: Fri, 14 Dec 2007 14:03:25 -0500 From: "Stacy McLaughlin" Subject: [Histonet] bone saws To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I'm looking for information on bone saws. It will primarily be used for femoral heads and knee caps. What kinds are you using? My Pathologist wants some kind of device that will immobilize the femoral heads so they don't roll around when cutting. Any ideas out there? As always your answers are appreciated! Stacy McLaughlin, HT Cooley Dickinson Hospital ------------------------------ Message: 2 Date: Fri, 14 Dec 2007 14:07:09 -0500 From: "Burrill, Jason" Subject: [Histonet] RE: 10% formaldehyde?? To: , Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E8F0ECE@shr-exch2.na01.crl.com> Content-Type: text/plain; charset="koi8-r" Dear Sara, According to the Dangerous Goods Regulations 10% formalin (4%formaldehyde) is not regulated by either the DOT or IATA.? The cut off for the percentage of formaldehyde in a solution that is considered a Dangerous Good and must be classified is ?10% formaldehyde.? So since 10% formalin is only 4% formaldehyde it is not regulated as a Dangerous Good but 10% formaldehyde is considered a "Aviation regulated liquid, n.o.s. UN3334" and subject to classification when shipping by air.? This is a common mistake for people who ship samples fixed in 10% formalin.? If you would like to discuss this further or would like additional references to confirm this please call or e-mail me. Regards, Jason Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** ------------------------------ Message: 3 Date: Fri, 14 Dec 2007 13:18:54 -0600 From: "Mike Pence" Subject: RE: [Histonet] bone saws To: "Stacy McLaughlin" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C809@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" This is the saw we use for just that purpose. http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Friday, December 14, 2007 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone saws Hi, I'm looking for information on bone saws. It will primarily be used for femoral heads and knee caps. What kinds are you using? My Pathologist wants some kind of device that will immobilize the femoral heads so they don't roll around when cutting. Any ideas out there? As always your answers are appreciated! Stacy McLaughlin, HT Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 14 Dec 2007 13:21:28 -0600 From: "Burton, Lynn" Subject: RE: [Histonet] Re: Pregnancy To: "Laurie Popp" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" My children are now 13,10, and almost 6 and my experience was the same. My last 2 children were higher risk because I was preeclamptic with the first and was diabetic with the last. I made a list in the lab of all the chemicals after I physically went through and read each MSDS. If anyone is interested I would happily fax it to you. Merry Christmas! Lynn Burton ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp Sent: Thu 12/13/2007 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Pregnancy Hi Histonetters, I actually just had a healthy baby girl 8/10/07 and was a high risk pregnancy ( diabetic) while finishing my HT and working full time in the histo lab at Mayo. I was advised by my OB to limit my formalin exposure, to limit my xylene exposure as much as possible, and to be very careful in special stains area. To also be careful of B-5 which we use for our bone marrows and the list goes on. Basically I was limited to cutting and embedding most days. Hope this helps! Laurie Popp, BA HT ( ASCP) and happy mommy to Kaiana :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 14 Dec 2007 16:58:50 -0500 From: Robert Schloesser Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed To: Cc: service@leica-microsystems.com, "Ernie.Oates@leica-microsystems.com" , Tino.Thakral@leica-microsystems.com Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi all Histotechs, We have a LEICA SM2000 R microtome. It worked fine for a few years but recently broke (the stage would not move anymore). We spend $2000 and over 3 month trying to get it repaired by LEICA. LEICAs customer service is completely incompetent and its technicians are even more incompetent (they came our more than 6 times - they even send someone from Chigaco here to DC to try to repair a simple microtome). Anyways, to make a sad story short I would STRONGLY advice everyone not to buy ANY products from LEICA that might require service. Furthermore, I would like to hear from you if there is a suggestion from the community what microtome to buy (since we have decided to throw our LEICA SM2000 in the trash). We need a microtome for cutting frozen brain tissue from rodents and primates. (usually 40um thick, sometimes up to 300um, sometimes thinner). We usually use STURKEY MICROTOME KNIFES. Any suggestions? Thank you Robert Robert J. Schloesser,MD visiting research fellow DHHS/NIH/NIMH/MAP/LMP Laboratory of Molecular Pathophysiology (LMP) Mood and Anxiety Disorders Program (MAP) National Institute of Mental Health (NIMH) Building 35/1C-912 35 Convent Drive Bethesda, MD 20892-3711 Phone: 301-451-8435 FAX: 301-480-0123 On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > My children are now 13,10, and almost 6 and my experience was the same. My > last 2 children were higher risk because I was preeclamptic with the first and > was diabetic with the last. I made a list in the lab of all the chemicals > after I physically went through and read each MSDS. If anyone is interested I > would happily fax it to you. > Merry Christmas! > Lynn Burton > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp > Sent: Thu 12/13/2007 5:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Pregnancy > > > > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 14 Dec 2007 16:21:55 -0600 From: Jan.Minshew@leica-microsystems.com Subject: Re: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed To: Robert Schloesser Cc: histonet@lists.utsouthwestern.edu, service@leica-microsystems.com, histonet-bounces@lists.utsouthwestern.edu, "Ernie.Oates@leica-microsystems.com" , Tino.Thakral@leica-microsystems.com Message-ID: Content-Type: text/plain; charset=US-ASCII Hello Dr. Schloesser, I am the Marketing Manager for the Leica SM2000 R and I would like to assure you that I was unaware of your difficulties. Please feel free to contact me so I can help resolve the obvious frustration you have encountered with the servicing of this instrument. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! Robert Schloesser h.gov> To Sent by: histonet-bounces@ cc lists.utsouthwest service@leica-microsystems.com, ern.edu "Ernie.Oates@leica-microsystems.com " 12/14/2007 03:58 >, PM Tino.Thakral@leica-microsystems.com Subject [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed Hi all Histotechs, We have a LEICA SM2000 R microtome. It worked fine for a few years but recently broke (the stage would not move anymore). We spend $2000 and over 3 month trying to get it repaired by LEICA. LEICAs customer service is completely incompetent and its technicians are even more incompetent (they came our more than 6 times - they even send someone from Chigaco here to DC to try to repair a simple microtome). Anyways, to make a sad story short I would STRONGLY advice everyone not to buy ANY products from LEICA that might require service. Furthermore, I would like to hear from you if there is a suggestion from the community what microtome to buy (since we have decided to throw our LEICA SM2000 in the trash). We need a microtome for cutting frozen brain tissue from rodents and primates. (usually 40um thick, sometimes up to 300um, sometimes thinner). We usually use STURKEY MICROTOME KNIFES. Any suggestions? Thank you Robert Robert J. Schloesser,MD visiting research fellow DHHS/NIH/NIMH/MAP/LMP Laboratory of Molecular Pathophysiology (LMP) Mood and Anxiety Disorders Program (MAP) National Institute of Mental Health (NIMH) Building 35/1C-912 35 Convent Drive Bethesda, MD 20892-3711 Phone: 301-451-8435 FAX: 301-480-0123 On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > My children are now 13,10, and almost 6 and my experience was the same. My > last 2 children were higher risk because I was preeclamptic with the first and > was diabetic with the last. I made a list in the lab of all the chemicals > after I physically went through and read each MSDS. If anyone is interested I > would happily fax it to you. > Merry Christmas! > Lynn Burton > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp > Sent: Thu 12/13/2007 5:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Pregnancy > > > > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 7 Date: Fri, 14 Dec 2007 17:15:50 -0600 From: Colleen Forster Subject: Re: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed To: Jan.Minshew@leica-microsystems.com Cc: histonet@lists.utsouthwestern.edu, Tino.Thakral@leica-microsystems.com, service@leica-microsystems.com, histonet-bounces@lists.utsouthwestern.edu, "Ernie.Oates@leica-microsystems.com" Message-ID: <47630EA6.30405@umn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed To Dr.Schloesser, Although I am sorry to hear that you have had trouble with your Leica rep. I have to say in their defense I have a very good one. They are always prompt to answer my phone calls and have always provided the service and support I need. I think it would have been better if you had contacted the main Leica company BEFORE you attempted to roast them on the histonet as Leica has a very good product and is a very reputable company. My 2 cents for the day!! Colleen Forster U of MN Jan.Minshew@leica-microsystems.com wrote: > Hello Dr. Schloesser, > > I am the Marketing Manager for the Leica SM2000 R and I would like to > assure you that I was unaware of your difficulties. Please feel free to > contact me so I can help resolve the obvious frustration you have > encountered with the servicing of this instrument. > > Best wishes, > > > Jan Minshew, HT/HTL(ASCP) > Marketing Manager > Leica Microsystems > Biosystems Division > 2345 Waukegan Road > Bannockburn, IL 60015 > > 800.248.0123 Toll Free > 847.405.7051 Direct > 847.405.6560 Fax > > www.leica-microsystems.com > > Click Here for this month's special offers! > > > > Robert Schloesser > > h.gov> To > Sent by: > histonet-bounces@ cc > lists.utsouthwest service@leica-microsystems.com, > ern.edu "Ernie.Oates@leica-microsystems.com > " > > 12/14/2007 03:58 >, > PM Tino.Thakral@leica-microsystems.com > Subject > [Histonet] WARNING ABOUT LEICA > MICROTOMES AND SERVICE and > Suggestions needed > > > > > > > > > > > Hi all Histotechs, > > We have a LEICA SM2000 R microtome. It worked fine for a few years but > recently broke (the stage would not move anymore). We spend $2000 and over > 3 > month trying to get it repaired by LEICA. LEICAs customer service is > completely incompetent and its technicians are even more incompetent (they > came our more than 6 times - they even send someone from Chigaco here to DC > to try to repair a simple microtome). > > Anyways, to make a sad story short I would STRONGLY advice everyone not to > buy ANY products from LEICA that might require service. > > Furthermore, I would like to hear from you if there is a suggestion from === message truncated === --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From carl.hobbs <@t> kcl.ac.uk Sun Dec 16 12:45:54 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sun Dec 16 12:46:19 2007 Subject: [Histonet] Leica microtomes, service and suggestion Message-ID: <002401c84013$e3748640$4101a8c0@carlba65530bda> Nice one, Kemlo! Leica, Nikon and Zeiss...to name 3 of the big ones in the UK ( AND their distributors) are very stretched. Fact. Why? Money Not explained to end-user, imho. All maintain a smug front, using denial to great effect Have to admit that, in UK, there are only 2 microscope guys who I respect: Nikon's UK London Rep...forgot his name( sorry, matey!) and Zeiss man Aubrey! The rest come and go...sigh. Carl From amosbrooks <@t> gmail.com Sun Dec 16 16:07:39 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Dec 16 16:07:50 2007 Subject: [Histonet] Leica Message-ID: <582736990712161407u40e49110wc78c6df993e1d72@mail.gmail.com> Hi, God help me I agree with Kelmo! (HEHE) I agree the tone of the message was a bit harsh to say the least. I also agree that the main company should have had a chance to rectify the situation before the flame came on. The fact is that the complaint on the Histonet got the desired attention and the problem will hopefully be fixed in short order. If the situation happened to not be unique it would be important for folks in the DC area to know that. We shouldn't castigate someone for airing a problem. We should help direct him to the resources to rectify the situation, like saying "Calm down & contact the headquarters." which is what many people did. Please know I am not kicking at Leica as I have an ANCIENT Leica microtome myself and the fact that it still works great & isn't a pile of rusted metal in a heap like some others I've seen is a tribute to the company's product. This situation has actually had the counter effect from my perspective. I see the company headquarters addressed the situation dang fast in spite of any regional difficulties. Good job Leica! Need I say more? All in all a sucess story, Amos From kmerriam2003 <@t> yahoo.com Mon Dec 17 06:47:49 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Dec 17 06:48:02 2007 Subject: [Histonet] frozen section temperature chart Message-ID: <914583.61045.qm@web50304.mail.re2.yahoo.com> Hi, Does anyone know where I can access a chart (it is for an SOP) that has a list of the recommended cryostat temperatures for cutting certain types of tissues. Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From Susan.Weber2 <@t> va.gov Mon Dec 17 07:41:44 2007 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Mon Dec 17 07:41:58 2007 Subject: [Histonet] bone saws In-Reply-To: References: Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB902276C9E@VHAV10MSGA1.v10.med.va.gov> Stacey- My last job was for a hospital which was primarily orthopedic; we cut four to five TKR and two to three THR specimens a day! There is a wonderful product which is a band bone saw which uses diamond blades instead of tooth type blades and water to keep the bone dust from flying. I used this all the time and as long as you use the DIAMOND blade it is quite safe. My distributor was Mar-Med and the Reps name is Ron Sienk. The phone number is (440) 572-5175. Thermo also has a holder with a hacksaw type blade (it is a double blade (I put two cassette lids, one at either end to separate the blades better, and created a perfect thickness for decal) it is called the Saw Bones and can be found in their catalog or on their web-site. We decaled the cut sections overnight in Surgipath Decal I (Formic Acid-Formalin) and Stephens Scientific Decal II (Chelating HCL) for the day or overnight if needed. This is just the right amount of decal for AVN bone pathology (not too much- not too little). Good Luck! Happy Holidays! Susan M. Weber, HT(ASCP) Louis Stokes Cleveland VA Medical Center Supervisor, Histology Laboratory (216) 791-3800 ext 6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Friday, December 14, 2007 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone saws Hi, I'm looking for information on bone saws. It will primarily be used for femoral heads and knee caps. What kinds are you using? My Pathologist wants some kind of device that will immobilize the femoral heads so they don't roll around when cutting. Any ideas out there? As always your answers are appreciated! Stacy McLaughlin, HT Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Mon Dec 17 08:06:55 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Dec 17 08:13:28 2007 Subject: [Histonet] frozen section temperature chart In-Reply-To: <914583.61045.qm@web50304.mail.re2.yahoo.com> References: <914583.61045.qm@web50304.mail.re2.yahoo.com> Message-ID: <6.2.3.4.1.20071217070346.01fb5858@algranth.inbox.email.arizona.edu> There was a chart like this in the procedure manual here when I came and it is from Reichert-Jung. Must have come in the operation manual for one of the cryostats they used to have here. Andi At 05:47 AM 12/17/2007, Kim Merriam wrote: >Hi, > >Does anyone know where I can access a chart (it is for an SOP) that >has a list of the recommended cryostat temperatures for cutting >certain types of tissues. > >Thanks in advance, >Kim > >Kim Merriam, MA, HT(ASCP) >Cambridge, MA > > > >____________________________________________________________________________________ >Never miss a thing. Make Yahoo your home page. >http://www.yahoo.com/r/hs >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From vazquezr <@t> ohsu.edu Mon Dec 17 08:38:17 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Dec 17 08:38:41 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed Message-ID: Not to burst your bubble, but I have a Leica cryostat. It is new and have had a lot of problems with it, they just have to figure out what the problem is. It could be a simple problem. The service and technician are awesome and ARE very knowledgable in what they are doing. They are very helpful and want us to be happy with a excellent product. Don't bash all, for just one simple lemon, make lemonade...Happy Monday!!! Robyn From mcauliff <@t> umdnj.edu Mon Dec 17 09:36:34 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Dec 17 09:36:49 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions needed In-Reply-To: References: Message-ID: <47669782.3040309@umdnj.edu> We had similar problems over 15 years ago (inept service, shipped us someone else's microtome, charged us for express shipping but shipped the equipment standard ground). We bought another manufacturer's confocal 'scope due to Leica's poor service. Obviously an ongoing problem. Geoff Robert Schloesser wrote: > Hi all Histotechs, > > We have a LEICA SM2000 R microtome. It worked fine for a few years but > recently broke (the stage would not move anymore). We spend $2000 and over 3 > month trying to get it repaired by LEICA. LEICAs customer service is > completely incompetent and its technicians are even more incompetent (they > came our more than 6 times - they even send someone from Chigaco here to DC > to try to repair a simple microtome). > > Anyways, to make a sad story short I would STRONGLY advice everyone not to > buy ANY products from LEICA that might require service. > > Furthermore, I would like to hear from you if there is a suggestion from the > community what microtome to buy (since we have decided to throw our LEICA > SM2000 in the trash). We need a microtome for cutting frozen brain tissue > from rodents and primates. (usually 40um thick, sometimes up to 300um, > sometimes thinner). We usually use STURKEY MICROTOME KNIFES. > > Any suggestions? Thank you > > Robert > > > Robert J. Schloesser,MD > visiting research fellow > DHHS/NIH/NIMH/MAP/LMP > > Laboratory of Molecular Pathophysiology (LMP) > Mood and Anxiety Disorders Program (MAP) > National Institute of Mental Health (NIMH) > > Building 35/1C-912 > 35 Convent Drive > Bethesda, MD 20892-3711 > Phone: 301-451-8435 > FAX: 301-480-0123 > > > > > On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > > >> My children are now 13,10, and almost 6 and my experience was the same. My >> last 2 children were higher risk because I was preeclamptic with the first and >> was diabetic with the last. I made a list in the lab of all the chemicals >> after I physically went through and read each MSDS. If anyone is interested I >> would happily fax it to you. >> Merry Christmas! >> Lynn Burton >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp >> Sent: Thu 12/13/2007 5:56 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Pregnancy >> >> >> >> Hi Histonetters, >> >> I actually just had a healthy baby girl 8/10/07 and was a high risk >> pregnancy ( diabetic) while finishing my HT and working full time in the >> histo lab at Mayo. I was advised by my OB to limit my formalin >> exposure, to limit my xylene exposure as much as possible, and to be >> very careful in special stains area. To also be careful of B-5 which we >> use for our bone marrows and the list goes on. Basically I was limited >> to cutting and embedding most days. >> >> Hope this helps! >> >> Laurie Popp, BA HT ( ASCP) >> and happy mommy to Kaiana :-) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From cmiller <@t> physlab.com Mon Dec 17 09:57:52 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Dec 17 09:57:54 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE andSuggestions needed In-Reply-To: References: Message-ID: <001501c840c5$9477e700$3402a8c0@plab.local> All I know is that Leica makes the best microtome hands down! Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Schloesser Sent: Friday, December 14, 2007 3:59 PM To: histonet@lists.utsouthwestern.edu Cc: service@leica-microsystems.com; Ernie.Oates@leica-microsystems.com; Tino.Thakral@leica-microsystems.com Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE andSuggestions needed Hi all Histotechs, We have a LEICA SM2000 R microtome. It worked fine for a few years but recently broke (the stage would not move anymore). We spend $2000 and over 3 month trying to get it repaired by LEICA. LEICAs customer service is completely incompetent and its technicians are even more incompetent (they came our more than 6 times - they even send someone from Chigaco here to DC to try to repair a simple microtome). Anyways, to make a sad story short I would STRONGLY advice everyone not to buy ANY products from LEICA that might require service. Furthermore, I would like to hear from you if there is a suggestion from the community what microtome to buy (since we have decided to throw our LEICA SM2000 in the trash). We need a microtome for cutting frozen brain tissue from rodents and primates. (usually 40um thick, sometimes up to 300um, sometimes thinner). We usually use STURKEY MICROTOME KNIFES. Any suggestions? Thank you Robert Robert J. Schloesser,MD visiting research fellow DHHS/NIH/NIMH/MAP/LMP Laboratory of Molecular Pathophysiology (LMP) Mood and Anxiety Disorders Program (MAP) National Institute of Mental Health (NIMH) Building 35/1C-912 35 Convent Drive Bethesda, MD 20892-3711 Phone: 301-451-8435 FAX: 301-480-0123 On 12/14/07 2:21 PM, "Burton, Lynn" wrote: > My children are now 13,10, and almost 6 and my experience was the same. My > last 2 children were higher risk because I was preeclamptic with the first and > was diabetic with the last. I made a list in the lab of all the chemicals > after I physically went through and read each MSDS. If anyone is interested I > would happily fax it to you. > Merry Christmas! > Lynn Burton > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp > Sent: Thu 12/13/2007 5:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Pregnancy > > > > Hi Histonetters, > > I actually just had a healthy baby girl 8/10/07 and was a high risk > pregnancy ( diabetic) while finishing my HT and working full time in the > histo lab at Mayo. I was advised by my OB to limit my formalin > exposure, to limit my xylene exposure as much as possible, and to be > very careful in special stains area. To also be careful of B-5 which we > use for our bone marrows and the list goes on. Basically I was limited > to cutting and embedding most days. > > Hope this helps! > > Laurie Popp, BA HT ( ASCP) > and happy mommy to Kaiana :-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From JMacDonald <@t> mtsac.edu Mon Dec 17 10:02:23 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Dec 17 10:02:44 2007 Subject: [Histonet] frozen section temperature chart In-Reply-To: <6.2.3.4.1.20071217070346.01fb5858@algranth.inbox.email.arizona.edu> Message-ID: There is also a chart in the Bancroft and Gamble book. Theory and Practice of Histological Techniques, page 102. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Andrea Grantham Sent by: histonet-bounces@lists.utsouthwestern.edu 12/17/2007 06:06 AM To Histonet cc Subject Re: [Histonet] frozen section temperature chart There was a chart like this in the procedure manual here when I came and it is from Reichert-Jung. Must have come in the operation manual for one of the cryostats they used to have here. Andi At 05:47 AM 12/17/2007, Kim Merriam wrote: >Hi, > >Does anyone know where I can access a chart (it is for an SOP) that >has a list of the recommended cryostat temperatures for cutting >certain types of tissues. > >Thanks in advance, >Kim > >Kim Merriam, MA, HT(ASCP) >Cambridge, MA > > > >____________________________________________________________________________________ >Never miss a thing. Make Yahoo your home page. >http://www.yahoo.com/r/hs >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Mon Dec 17 10:05:16 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Dec 17 10:05:17 2007 Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE andSuggestions needed In-Reply-To: References: Message-ID: <001601c840c6$9d5eff10$3402a8c0@plab.local> We all have our days. It takes a gracious person to apologize, I find it refreshing. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Schloesser Sent: Saturday, December 15, 2007 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE andSuggestions needed Dear all, I would like to use this email to APOLOGIZE for, but also explain the motives for my earlier email warning about LEICA service in particular our experience with our LEICA SM200R microtome. First of all, I would like to apologize because I do agree that the wording of this email was not appropriate for this forum. The reason for the email was an extreme level of frustration with our Leica service technicians here at NIH: The group of techs servicing our LEICA SM2000R were unable to repair the microtome for over 9 weeks and it is still not functional. We paid $1600 for ?urgent? service, but even after 7 repair trials the mechanism that moves the stage keeps on failing after little use or (yesterday) the handle fell off. This has caused us a tremendous loss of valuable time as well as money hence my frustration induced overreaction. The many hours we spend talking to various levels of LEICA service hotlines did not lead to any appropriate reaction and the communication with the manager of our service technician team was very unproductive and at times unpleasant, again leading to a buildup of frustration that sparked in my inappropriate misuse of this forum. Please note 1. I agree that ?when functional? the LEICA SM2000R is a very convenient to use microtome (I have used it for over 3 years) 2. LEICA SM2000R marketing representatives called and emailed me less than 15 minutes after my original email and initiated a phone conference with the technical service headquarter. LEICA reviewed our case within minutes and confirmed that it was an unusual case of unusually bad service. I would like to use this email to thank LEICAs headquarter for this quick response and express my hope and optimism that the promised changes will be made at the our service department and we will not have to wait for another 9 weeks before our microtome starts working again. 3. Again, I agree that my previous email was not appropriately worded since it was written out of frustration. I should have given this more time for thought. Please accept these apologies since I highly value this forum and its community Robert On 12/15/07 10:49 AM, "Jack Ratliff" wrote: > I am compelled to reply to this disturbing message as I STRONGLY feel very > disturbed that ANYONE would actually use this forum to send such a dirty and > misleading message for the eyes of thousands of histonet readers! > > I have USED, PURCHASED, and RECOMMENDED the purchase/use of LEICA equipment > ever since I entered the field 11 years ago. In fact, I continue to purchase, > use, and recommend the use of this equipment to this very day and I have NEVER > had such an issue as described in the original message! Additionally, I > support this equipment because of the many, many positive experiences that I > have had with both the equipment and the representatives over these years! > > I cannot even fathom the problems as suggested as it is very true that the > equipment is well engineered. Lastly, in all my dealings with LEICA > representatives over the years, I NEVER could imagine that the service would > be as the original message has described. In my experience, these > representatives are true professionals, unlike the authors comments, tactics, > and/or motives in the original message on this subject. > > Jack Ratliff > > PS If your microtome has not left for the dump yet, I would LOVE to take it > off your hands! > > > > > > > > > > >> > Date: Fri, 14 Dec 2007 16:58:50 -0500 >> > From: schloesr@mail.nih.gov >> > To: histonet@lists.utsouthwestern.edu >> > CC: service@leica-microsystems.com; Ernie.Oates@leica-microsystems.com; >> Tino.Thakral@leica-microsystems.com >> > Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and >> Suggestions needed >> > >> > Hi all Histotechs, >> > >> > We have a LEICA SM2000 R microtome. It worked fine for a few years but >> > recently broke (the stage would not move anymore). We spend $2000 and over 3 >> > month trying to get it repaired by LEICA. LEICAs customer service is >> > completely incompetent and its technicians are even more incompetent (they >> > came our more than 6 times - they even send someone from Chigaco here to DC >> > to try to repair a simple microtome). >> > >> > Anyways, to make a sad story short I would STRONGLY advice everyone not to >> > buy ANY products from LEICA that might require service. >> > >> > Furthermore, I would like to hear from you if there is a suggestion from >> the >> > community what microtome to buy (since we have decided to throw our LEICA >> > SM2000 in the trash). We need a microtome for cutting frozen brain tissue >> > from rodents and primates. (usually 40um thick, sometimes up to 300um, >> > sometimes thinner). We usually use STURKEY MICROTOME KNIFES. >> > >> > Any suggestions? Thank you >> > >> > Robert >> > >> > >> > Robert J. Schloesser,MD >> > visiting research fellow >> > DHHS/NIH/NIMH/MAP/LMP >> > >> > Laboratory of Molecular Pathophysiology (LMP) >> > Mood and Anxiety Disorders Program (MAP) >> > National Institute of Mental Health (NIMH) >> > >> > Building 35/1C-912 >> > 35 Convent Drive >> > Bethesda, MD 20892-3711 >> > Phone: 301-451-8435 >> > FAX: 301-480-0123 >> > >> > >> > >> > >> > On 12/14/07 2:21 PM, "Burton, Lynn" wrote: >> > >>> > > My children are now 13,10, and almost 6 and my experience was the same. My >>> > > last 2 children were higher risk because I was preeclamptic with the >>> first and >>> > > was diabetic with the last. I made a list in the lab of all the >>> chemicals >>> > > after I physically went through and read each MSDS. If anyone is >>> interested I >>> > > would happily fax it to you. >>> > > Merry Christmas! >>> > > Lynn Burton >>> > > >>> > > ________________________________ >>> > > >>> > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp >>> > > Sent: Thu 12/13/2007 5:56 PM >>> > > To: histonet@lists.utsouthwestern.edu >>> > > Subject: [Histonet] Re: Pregnancy >>> > > >>> > > >>> > > >>> > > Hi Histonetters, >>> > > >>> > > I actually just had a healthy baby girl 8/10/07 and was a high risk >>> > > pregnancy ( diabetic) while finishing my HT and working full time in the >>> > > histo lab at Mayo. I was advised by my OB to limit my formalin >>> > > exposure, to limit my xylene exposure as much as possible, and to be >>> > > very careful in special stains area. To also be careful of B-5 which we >>> > > use for our bone marrows and the list goes on. Basically I was limited >>> > > to cutting and embedding most days. >>> > > >>> > > Hope this helps! >>> > > >>> > > Laurie Popp, BA HT ( ASCP) >>> > > and happy mommy to Kaiana :-) >>> > > >>> > > >>> > > _______________________________________________ >>> > > Histonet mailing list >>> > > Histonet@lists.utsouthwestern.edu >>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > > >>> > > >>> > > _______________________________________________ >>> > > Histonet mailing list >>> > > Histonet@lists.utsouthwestern.edu >>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > The best games are on Xbox 360. Click here for a special offer on an Xbox 360 > Console. Get it now! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From karenadams <@t> comcast.net Mon Dec 17 10:18:37 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Mon Dec 17 10:18:50 2007 Subject: [Histonet] selling microtomes Message-ID: <121720071618.14786.4766A15D0006B33F000039C222070029539C030E0B0E020A9D0E05@comcast.net> I have 2 old American Optical rotary microtomes....does anyone have any contacts for selling them? -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From kmerriam2003 <@t> yahoo.com Mon Dec 17 10:30:47 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Dec 17 10:31:01 2007 Subject: [Histonet] frozen section temperature chart Message-ID: <863168.31740.qm@web50305.mail.re2.yahoo.com> Thanks so much for all of the responses I got. It seems to me that the best way to get this chart is in your cryostat operators manual. The Leica website has many manuals on-line and I managed to find one here (page 52): http://www.histo-solutions.com/pdfs.nsf/(ALLIDs)/8E60A5111F8D6CC3C12573920052A4C6/$FILE/Leica_CM1950_Manual_1v1_EN.pdf This seems to be an issue that should really be addressed in more textbooks. I looked through all of the ones that I had and the only textbook that mentioned it was Bancroft, but this chart was very generic with very wide temperature ranges. Thanks again, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Jennifer MacDonald To: Andrea Grantham Cc: Histonet ; histonet-bounces@lists.utsouthwestern.edu Sent: Monday, December 17, 2007 11:02:23 AM Subject: Re: [Histonet] frozen section temperature chart There is also a chart in the Bancroft and Gamble book. Theory and Practice of Histological Techniques, page 102. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Andrea Grantham Sent by: histonet-bounces@lists.utsouthwestern.edu 12/17/2007 06:06 AM To Histonet cc Subject Re: [Histonet] frozen section temperature chart There was a chart like this in the procedure manual here when I came and it is from Reichert-Jung. Must have come in the operation manual for one of the cryostats they used to have here. Andi At 05:47 AM 12/17/2007, Kim Merriam wrote: >Hi, > >Does anyone know where I can access a chart (it is for an SOP) that >has a list of the recommended cryostat temperatures for cutting >certain types of tissues. > >Thanks in advance, >Kim > >Kim Merriam, MA, HT(ASCP) >Cambridge, MA > > > >____________________________________________________________________________________ >Never miss a thing. Make Yahoo your home page. >http://www.yahoo.com/r/hs >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From hej01 <@t> health.state.ny.us Mon Dec 17 12:32:54 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Dec 17 12:33:07 2007 Subject: [Histonet] Bouin's fixation for mouse nasal passages Message-ID: Hi Histonetters, How long should mouse nasal passages be fixed in Bouin solution before cutting in & processing? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From mwich <@t> 7thwavelabs.com Mon Dec 17 12:48:38 2007 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Mon Dec 17 12:48:50 2007 Subject: [Histonet] ED1 staining in rat Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486B65@7THWAVE-SERVER.7thwave.local> Does anyone have an IHC protocol for ED1 staining in formalin-fixed paraffin sections of rat tissue (specifically rat brain)? I've noticed several mouse anti-rat antibodies available, but I'm not really sure where to start. Any suggestions would be much appreciated. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From Samuel_Perry <@t> DFCI.HARVARD.EDU Mon Dec 17 12:24:14 2007 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Mon Dec 17 12:51:19 2007 Subject: [Histonet] Desmin Message-ID: Hi All, I am trying to perform IHC staining using the Dako envision+ kit on mouse and human tissue. I am looking for an anti-desmin antibody which has been well published and characterized in the past. There seems to be several rabbit polyclonal antibodies out there by abcam, anyone have luck with these? I look forward to hearing peoples responses. Thanks -Sam Perry Dana Farber Cancer Institute Research Technician The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From Linke_Noelle <@t> Allergan.com Mon Dec 17 12:58:01 2007 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Mon Dec 17 12:58:24 2007 Subject: [Histonet] ED1 staining in rat In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486B65@7THWAVE-SERVER.7thwave.local> Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527CFED@IRMAIL132.irvine.allergan.com> That's a pretty easy one to get working.... ED1 from Serotec (cat# MCA341R), 1:400 dilution, citrate pretreatment (this Ab is pretty strong, may work without it) 1 hour incubation. Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Monday, December 17, 2007 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ED1 staining in rat Does anyone have an IHC protocol for ED1 staining in formalin-fixed paraffin sections of rat tissue (specifically rat brain)? I've noticed several mouse anti-rat antibodies available, but I'm not really sure where to start. Any suggestions would be much appreciated. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Dec 18 01:03:57 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Dec 18 01:04:15 2007 Subject: AW: [Histonet] Desmin In-Reply-To: Message-ID: <001401c84144$29ea9480$eeeea8c0@dielangs.at> We use monoclonal mouse Desmin D33 from Dako, 1:100 on Ventana Benchmark (pH 8 retrieval; 24 min inkubation). Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Perry, Samuel Gesendet: Montag, 17. Dezember 2007 19:24 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Desmin Hi All, I am trying to perform IHC staining using the Dako envision+ kit on mouse and human tissue. I am looking for an anti-desmin antibody which has been well published and characterized in the past. There seems to be several rabbit polyclonal antibodies out there by abcam, anyone have luck with these? I look forward to hearing peoples responses. Thanks -Sam Perry Dana Farber Cancer Institute Research Technician The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpiche-grocki <@t> wtbyhosp.org Tue Dec 18 11:40:41 2007 From: jpiche-grocki <@t> wtbyhosp.org (Piche-Grocki, Jessica) Date: Tue Dec 18 11:41:43 2007 Subject: [Histonet] Cold Plate Message-ID: <0A57D6AEAE4CBA4A984D27257160A72D01069D22@win03exchange01.wtbyhosp.org> Hi, Just wanted to say thanks to everyone who shared information on cold plate QC. Thanks again, Jessica CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From benardsolomona <@t> yahoo.com Tue Dec 18 11:57:07 2007 From: benardsolomona <@t> yahoo.com (Solomon Adebayo) Date: Tue Dec 18 11:57:38 2007 Subject: [Histonet] Schiff's reagent In-Reply-To: <6.2.3.4.1.20071203155105.01fc65a8@algranth.inbox.email.arizona.edu> Message-ID: <450981.38163.qm@web36105.mail.mud.yahoo.com> This is a rather late reply to the querry by Andrea. The shell life and stability of schiff's reagent depend on the formular and mode of preparation. we use a modified schiff's reagent with a shell life of more than 12 months. It still maintain its potency and very stable. Benard Solomon Pathology Dept., Unilorin Teaching Hospital, Ilorin, Kwara State, Nigeria www.histosearch/homepage/benardsolomon --- Andrea Grantham wrote: > I'm using a Schiff's from Newcomer Supply that > has a pretty long shelf life. It can be stored at > RT and can be used several times. > > We always put the "used" Schiff's into a > different bottle as to not contaminate the unused > solution. > > Andi Grantham > > > > > At 03:29 PM 12/3/2007, Rene J Buesa wrote: > >It has to be kept in the refrigerator, and yes, it > can be used several times. > > You can test how is working by adding a few > > drops of the Schiff's reagent to NBF, it it > > reacts quickly with a magenta color, is working > OK, if not, discard it. > > Ren? J. > > > >Taben Hale wrote: > > What is the shelf-life and stability of Schiff's > reagent? Can it be used > >multiple times? > > > >-- > >Taben M Hale, PhD > >Postdoctoral Fellow > >Universite de Montreal > >Dept Pharmacologie > >(514)343-6111 ext. 4968 > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > >--------------------------------- > >Be a better sports nut! Let your teams follow > >you with Yahoo Mobile. Try it now. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell > Biology & Anatomy : > : Sr. Research Specialist University of > Arizona : > : (office: AHSC 4212) P.O. Box 245044 > : > : (voice: 520-626-4415) Tucson, AZ > 85724-5044 USA : > : (FAX: 520-626-2097) (email: > algranth@u.arizona.edu) : > :...................................................................: > > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From bonnie.kaliko <@t> roche.com Tue Dec 18 12:12:04 2007 From: bonnie.kaliko <@t> roche.com (Kaliko, Bonnie) Date: Tue Dec 18 12:12:36 2007 Subject: [Histonet] Please unsubscribe Message-ID: Best Regards, Bonnie L. Kaliko Non- Clinical Safety Hoffmann La Roche Nutley, NJ 07110 Tel. 973 235-6464 Fax. 973 235-4710 e-mail: bonnie.kaliko@roche.com From pinetree110567 <@t> yahoo.com Tue Dec 18 12:15:34 2007 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Tue Dec 18 12:15:46 2007 Subject: [Histonet] Cassette Printer (corection) Message-ID: <227503.30035.qm@web44913.mail.sp1.yahoo.com> I am sorry, but on my last post I mistakenly stated that Thermo produced cassette and slide printers as well as laser slide writers for RA Lamb and TBS. I received a few emails from histonetters who correctly informed me that it is actually RA Lamb who makes the writers for Thermo and TBS. I am sorry if I misled anyone. Have a great holiday season!!! Laurie ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From amylee779 <@t> yahoo.com Tue Dec 18 12:23:51 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Tue Dec 18 12:24:03 2007 Subject: [Histonet] Thanks! Message-ID: <299714.35157.qm@web38014.mail.mud.yahoo.com> Thank you very much to all who recommended slide printer and cassette printer to me! Happy holiday! Amy --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From talulahgosh <@t> gmail.com Tue Dec 18 12:46:28 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Dec 18 12:46:43 2007 Subject: [Histonet] Please unsubscribe In-Reply-To: References: Message-ID: DO NOT WRITE TO THE LIST. CLICK ON THE LINK BELOW TO UNSUBSCRIBE. DO NOT CLICK ON THE LINK THAT WRITES TO THE LIST. On Dec 18, 2007 1:12 PM, Kaliko, Bonnie wrote: > > > > > Best Regards, > Bonnie L. Kaliko > Non- Clinical Safety > Hoffmann La Roche > Nutley, NJ 07110 > Tel. 973 235-6464 > Fax. 973 235-4710 > e-mail: bonnie.kaliko@roche.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- crow tom mike and joel trapped in space the endless void we've got movie sign! mst3k haiku brought to you by joe wiencis From dmccaig <@t> ckha.on.ca Tue Dec 18 14:35:20 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Dec 18 14:39:58 2007 Subject: [Histonet] SYNOPTIC REPORTING Message-ID: Hi Would anyone be interested in forwarding me copies of any synoptic reports you have in use. We are in the process of developing these reports and would appreciate any help. Thanks Diana McCaig From mpence <@t> grhs.net Tue Dec 18 15:09:21 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Dec 18 15:09:42 2007 Subject: [Histonet] SYNOPTIC REPORTING In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C810@IS-E2K3.grhs.net> Hi Diana, This is a site we have used, then we just cut and paste into our text field of our report. Works Great! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, December 18, 2007 2:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SYNOPTIC REPORTING Hi Would anyone be interested in forwarding me copies of any synoptic reports you have in use. We are in the process of developing these reports and would appreciate any help. Thanks Diana McCaig _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peddinti_2002us <@t> yahoo.co.in Tue Dec 18 15:23:39 2007 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Tue Dec 18 15:23:54 2007 Subject: [Histonet] Chart for cryostat Message-ID: <555015.55640.qm@web8709.mail.in.yahoo.com> Most of the manufacturers they provide the chart with operator manual. Thanks , Kamal --------------------------------- Forgot the famous last words? Access your message archive online. Click here. From mpence <@t> grhs.net Tue Dec 18 15:32:45 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Dec 18 15:32:57 2007 Subject: [Histonet] SYNOPTIC REPORTING In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C810@IS-E2K3.grhs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C812@IS-E2K3.grhs.net> http://www.urmc.rochester.edu/path/zqu/cap/webform1.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, December 18, 2007 3:09 PM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SYNOPTIC REPORTING Hi Diana, This is a site we have used, then we just cut and paste into our text field of our report. Works Great! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, December 18, 2007 2:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SYNOPTIC REPORTING Hi Would anyone be interested in forwarding me copies of any synoptic reports you have in use. We are in the process of developing these reports and would appreciate any help. Thanks Diana McCaig _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Tue Dec 18 15:26:37 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Dec 18 17:20:44 2007 Subject: [Histonet] testing Message-ID: <817C2761C5A1394180709AEEDB775B7E042F8F55@NASEV03.hca.corpad.net> Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com From Carmen.Wynn <@t> us.astellas.com Tue Dec 18 17:28:30 2007 From: Carmen.Wynn <@t> us.astellas.com (Wynn, Carmen) Date: Tue Dec 18 17:29:32 2007 Subject: [Histonet] RE: ED1 staining in rat In-Reply-To: <20071218181747.81FE51C98078@mail170-va3.bigfish.com> Message-ID: Hello Michele, I have also had good results with the AbD Serotec's mouse anti-rat CD68 (ED1) in rejected grafts of FFPE rat heart and kidneys at 1:100 dilution. I used Trypsin Grade II (sigma cat#7168) for 30 minutes at 37C. I didn't have to use any HIER but as Noelle stated before...it is a pretty hardy antibody. Carmen Wynn, M.S., Senior Scientist Astellas Research Institute of America, LLC. (ARIA) Illinois Science and Technology Park 8045 Lamon Ave Skokie, IL 60077 Direct: 847-933-7419 Main: 847-933-7400 Fax: 847-933-7401 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, December 18, 2007 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 49, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Bouin's fixation for mouse nasal passages (Helen E Johnson) 2. ED1 staining in rat (Michele Wich) 3. Desmin (Perry, Samuel) 4. RE: ED1 staining in rat (Linke_Noelle) 5. AW: [Histonet] Desmin (Gudrun Lang) 6. Cold Plate (Piche-Grocki, Jessica) 7. Re: Schiff's reagent (Solomon Adebayo) ---------------------------------------------------------------------- Message: 1 Date: Mon, 17 Dec 2007 13:32:54 -0500 From: Helen E Johnson Subject: [Histonet] Bouin's fixation for mouse nasal passages To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, How long should mouse nasal passages be fixed in Bouin solution before cutting in & processing? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ Message: 2 Date: Mon, 17 Dec 2007 12:48:38 -0600 From: "Michele Wich" Subject: [Histonet] ED1 staining in rat To: Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486B65@7THWAVE-SERVER.7thwave.local> Content-Type: text/plain; charset="us-ascii" Does anyone have an IHC protocol for ED1 staining in formalin-fixed paraffin sections of rat tissue (specifically rat brain)? I've noticed several mouse anti-rat antibodies available, but I'm not really sure where to start. Any suggestions would be much appreciated. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ------------------------------ Message: 3 Date: Mon, 17 Dec 2007 13:24:14 -0500 From: "Perry, Samuel" Subject: [Histonet] Desmin To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi All, I am trying to perform IHC staining using the Dako envision+ kit on mouse and human tissue. I am looking for an anti-desmin antibody which has been well published and characterized in the past. There seems to be several rabbit polyclonal antibodies out there by abcam, anyone have luck with these? I look forward to hearing peoples responses. Thanks -Sam Perry Dana Farber Cancer Institute Research Technician The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. ------------------------------ Message: 4 Date: Mon, 17 Dec 2007 10:58:01 -0800 From: "Linke_Noelle" Subject: RE: [Histonet] ED1 staining in rat To: "Michele Wich" , Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527CFED@IRMAIL132.irvine.allergan.com> Content-Type: text/plain; charset="iso-8859-1" That's a pretty easy one to get working.... ED1 from Serotec (cat# MCA341R), 1:400 dilution, citrate pretreatment (this Ab is pretty strong, may work without it) 1 hour incubation. Noelle Nolle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Monday, December 17, 2007 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ED1 staining in rat Does anyone have an IHC protocol for ED1 staining in formalin-fixed paraffin sections of rat tissue (specifically rat brain)? I've noticed several mouse anti-rat antibodies available, but I'm not really sure where to start. Any suggestions would be much appreciated. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 18 Dec 2007 08:03:57 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Desmin To: "'Perry, Samuel'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <001401c84144$29ea9480$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" We use monoclonal mouse Desmin D33 from Dako, 1:100 on Ventana Benchmark (pH 8 retrieval; 24 min inkubation). Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Ursprngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Perry, Samuel Gesendet: Montag, 17. Dezember 2007 19:24 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Desmin Hi All, I am trying to perform IHC staining using the Dako envision+ kit on mouse and human tissue. I am looking for an anti-desmin antibody which has been well published and characterized in the past. There seems to be several rabbit polyclonal antibodies out there by abcam, anyone have luck with these? I look forward to hearing peoples responses. Thanks -Sam Perry Dana Farber Cancer Institute Research Technician The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 18 Dec 2007 12:40:41 -0500 From: "Piche-Grocki, Jessica" Subject: [Histonet] Cold Plate To: Message-ID: <0A57D6AEAE4CBA4A984D27257160A72D01069D22@win03exchange01.wtbyhosp.org> Content-Type: text/plain; charset="iso-8859-1" Hi, Just wanted to say thanks to everyone who shared information on cold plate QC. Thanks again, Jessica CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ------------------------------ Message: 7 Date: Tue, 18 Dec 2007 09:57:07 -0800 (PST) From: Solomon Adebayo Subject: Re: [Histonet] Schiff's reagent To: Andrea Grantham , histonet@lists.utsouthwestern.edu Message-ID: <450981.38163.qm@web36105.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This is a rather late reply to the querry by Andrea. The shell life and stability of schiff's reagent depend on the formular and mode of preparation. we use a modified schiff's reagent with a shell life of more than 12 months. It still maintain its potency and very stable. Benard Solomon Pathology Dept., Unilorin Teaching Hospital, Ilorin, Kwara State, Nigeria www.histosearch/homepage/benardsolomon --- Andrea Grantham wrote: > I'm using a Schiff's from Newcomer Supply that > has a pretty long shelf life. It can be stored at > RT and can be used several times. > > We always put the "used" Schiff's into a > different bottle as to not contaminate the unused > solution. > > Andi Grantham > > > > > At 03:29 PM 12/3/2007, Rene J Buesa wrote: > >It has to be kept in the refrigerator, and yes, it > can be used several times. > > You can test how is working by adding a few > > drops of the Schiff's reagent to NBF, it it > > reacts quickly with a magenta color, is working > OK, if not, discard it. > > Ren J. > > > >Taben Hale wrote: > > What is the shelf-life and stability of Schiff's > reagent? Can it be used > >multiple times? > > > >-- > >Taben M Hale, PhD > >Postdoctoral Fellow > >Universite de Montreal > >Dept Pharmacologie > >(514)343-6111 ext. 4968 > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > >--------------------------------- > >Be a better sports nut! Let your teams follow > >you with Yahoo Mobile. Try it now. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell > Biology & Anatomy : > : Sr. Research Specialist University of > Arizona : > : (office: AHSC 4212) P.O. Box 245044 > : > : (voice: 520-626-4415) Tucson, AZ > 85724-5044 USA : > : (FAX: 520-626-2097) (email: > algranth@u.arizona.edu) : > :...................................................................: > > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________________________________________________ ____________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 49, Issue 19 **************************************** From nfournier <@t> sasktel.net Tue Dec 18 22:33:21 2007 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Tue Dec 18 22:33:35 2007 Subject: [Histonet] rat perfusion Message-ID: <000801c841f8$49bee040$d995c5d8@NEIL> I have decided that our lab needs to revise our current protocol for rat intracardiac perfusion b/c the quality of perfused tissue has been reduced significantly over the last few months. I also thought it would be best to call upon the significant expertise of many of the histonet members. I hope that you might have some important suggestions and comments for us. I also apologize a priori for my naivety on this subject. Our protocol is as follows (for an adult rat typically 300 to 500 g): 1) Animals euthanized with a high dose of Euthanyl. 2) Perfuse cold 0.9% physiological saline (on ice). Using a perfusion pump with an 17 or 18 gauge blunted needle inserted into the ascending aorta. (I used to use 15 or 16 gauge needles) The heart is clamped to secure the needle tightly in place and the right atrium cut. Typically we perfuse between 300 and 500 ml over a five to ten minute period. 3) Perfuse cold 4% paraformaldehyde (on ice). (e.g., 40 g of paraformaldehyde dissolved in 800 ml warm PBS, filtered, cooled, and pH 7.4 with solution toped to a final volume of 1000 ml. All solutions are made up fresh, usually the day before or at least a few days before perfusion is required. Solutions are discarded if older than one week). The flow rate is 300 and 500 ml over a 10 to 15 minute period. *** I have noticed from time to time that when some students are perfusing some of the animals do not exhibit the typical spontaneous movements during fixation. However, sometimes the body will still appear rigid and fixed despite not showing these typical movement responses. I am uncertain if the classic "formalin dance" has to be a characteristic of a good whole body perfusion and was wondering what other people's take on this situation is. 4) Brains are removed and postfixed for 48 hrs before being sectioned on a Vibrating microtome at 50 microns. Tissue sections are usually stored in a ethylene glycol based cryoprotectant solution at a temperature between 20 and 25 degree C using a protocol identical to Gloria Hoffman's procedure (Peptides 1986, 7(1): 155-159.) Before I developed significant allergies, which makes my contact with the laboratory animals now at a minimal (poetic justice), I never had the same degree of problems that we seem to be observing lately. Although sectioning is not problematic with this tissue, the quality of tissue after IHC staining is generally poor (i.e., significant tearing of the tissue throughout etc.). Tissue is stained using the free-floating method and tissue is transferred from rinse to rinse using Netwells. I realize that this method in itself can contribute to some damage. I am not completely sure what the problem is but the consistency of this problem across animals and multiple experiments suggests to me one possibility is the actual perfusion procedure and that tissue integrity is minimal. Any suggestions and comments are welcomed. lastly, I was hoping that someone might know of a good book that discusses perfusion techniques in greater detail. Hopefully with some pictures as this is the often the best way to teach students (including myself). Thanks for the help, N From rosa_113 <@t> hotmail.com Wed Dec 19 07:16:56 2007 From: rosa_113 <@t> hotmail.com (Rosa Bryant) Date: Wed Dec 19 07:17:14 2007 Subject: [Histonet] (no subject) Message-ID: I can not seem to get my histonet digest, is anyone else having problems?Rosa Fields _________________________________________________________________ i?m is proud to present Cause Effect, a series about real people making a difference. http://im.live.com/Messenger/IM/MTV/?source=text_Cause_Effect From histotechkb <@t> gmail.com Wed Dec 19 08:03:07 2007 From: histotechkb <@t> gmail.com (Kristen Broomall) Date: Wed Dec 19 08:03:19 2007 Subject: [Histonet] Rat T Cell Marker Message-ID: <667c97ab0712190603n3a0320fcpa9a5aff770ff4239@mail.gmail.com> Good Morning all, I was wondering if anyone had suggestions on an antibody to detect T cells (only, no B cells) in formalin fixed, paraffin embedded rat thymus and spleen. I have a Pan T Cell marker that is working well in the spleen, but so-so in the thymus, so I think that it is only staining the mature T cells, and not immature cells (which of course, is what we also need to see). I've been reading up alot on T cells and trying to figure this out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear our deadline for getting staining done is shorter than the time I have to educate myself properly! Any suggestions would be greatly appreciated! Happy Holidays, Kristen Broomall, HT (ASCP) histotechkb@gmail.com From mcauliff <@t> umdnj.edu Wed Dec 19 09:58:27 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Dec 19 09:58:34 2007 Subject: [Histonet] rat perfusion In-Reply-To: <000801c841f8$49bee040$d995c5d8@NEIL> References: <000801c841f8$49bee040$d995c5d8@NEIL> Message-ID: <47693FA3.3070402@umdnj.edu> Hi Neil: Your first problem is cold perfusate.Why?? All this does is constrict the blood vessels and imped fixation. The concept that cold fixative slows down autolysis and improves fixation is a myth What really happens is that the reaction of the fix with the tissue is slowed. Yes, there are some instances when cold fix is better, but this is not one of them. There is no need to use so much saline washout; you are using well over 10X the circulating blood volume. What you are doing is waiting 5 to 10 minutes to begin fixation! You will never wash out all of the blood, it is not necessary so don't try. Less than 100 ml is plenty. As soon as the nose blanches,switch to fix. Room temperature fix, not cold! Use a 15 or 16 gauge needle, the others are too small to achieve adequate flow. When you clamp the heart you may be impeding the flow of fixative due to misplaced clamp, try holding the needle in place and see if that improves your results. I have had perfusions that looked "good" but gave mediocre morphology and perfusions that looked "bad" with great morphology. I would expect the 'dance' ~90% of the time. I would slice the brain for post fixation, unless you must cut through the whole brain. I think the tearing problem is due to inadequate fixation. The steps above should fix (ha!) it. Geoff Neil Fournier wrote: > I have decided that our lab needs to revise our current protocol for rat intracardiac perfusion b/c the quality of perfused tissue has been reduced significantly over the last few months. I also thought it would be best to call upon the significant expertise of many of the histonet members. I hope that you might have some important suggestions and comments for us. I also apologize a priori for my naivety on this subject. > > Our protocol is as follows (for an adult rat typically 300 to 500 g): > > 1) Animals euthanized with a high dose of Euthanyl. > > 2) Perfuse cold 0.9% physiological saline (on ice). Using a perfusion pump with an 17 or 18 gauge blunted needle inserted into the ascending aorta. (I used to use 15 or 16 gauge needles) The heart is clamped to secure the needle tightly in place and the right atrium cut. Typically we perfuse between 300 and 500 ml over a five to ten minute period. > > 3) Perfuse cold 4% paraformaldehyde (on ice). (e.g., 40 g of paraformaldehyde dissolved in 800 ml warm PBS, filtered, cooled, and pH 7.4 with solution toped to a final volume of 1000 ml. All solutions are made up fresh, usually the day before or at least a few days before perfusion is required. Solutions are discarded if older than one week). The flow rate is 300 and 500 ml over a 10 to 15 minute period. > > *** I have noticed from time to time that when some students are perfusing some of the animals do not exhibit the typical spontaneous movements during fixation. However, sometimes the body will still appear rigid and fixed despite not showing these typical movement responses. I am uncertain if the classic "formalin dance" has to be a characteristic of a good whole body perfusion and was wondering what other people's take on this situation is. > > 4) Brains are removed and postfixed for 48 hrs before being sectioned on a Vibrating microtome at 50 microns. Tissue sections are usually stored in a ethylene glycol based cryoprotectant solution at a temperature between 20 and 25 degree C using a protocol identical to Gloria Hoffman's procedure (Peptides 1986, 7(1): 155-159.) > > Before I developed significant allergies, which makes my contact with the laboratory animals now at a minimal (poetic justice), I never had the same degree of problems that we seem to be observing lately. Although sectioning is not problematic with this tissue, the quality of tissue after IHC staining is generally poor (i.e., significant tearing of the tissue throughout etc.). Tissue is stained using the free-floating method and tissue is transferred from rinse to rinse using Netwells. I realize that this method in itself can contribute to some damage. I am not completely sure what the problem is but the consistency of this problem across animals and multiple experiments suggests to me one possibility is the actual perfusion procedure and that tissue integrity is minimal. > > Any suggestions and comments are welcomed. lastly, I was hoping that someone might know of a good book that discusses perfusion techniques in greater detail. Hopefully with some pictures as this is the often the best way to teach students (including myself). > > Thanks for the help, > > N > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From koellingr <@t> comcast.net Wed Dec 19 10:07:53 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Dec 19 10:08:07 2007 Subject: [Histonet] Rat T Cell Marker Message-ID: <121920071607.14295.476941D900030D02000037D722070206539D09020704040A0105@comcast.net> Kristen, what "pan T cell marker" are you using? Also, what do you define as an "immature" T cell? I've used BD Pharmingen mouse anti-rat CD3 in FFPE thymus with citrate retrieval and that will target the CD3 complex but a few other subsets of cells other than T-cells can express CD3. Also have used BD Pharmingen mouse anti-rat alpha/beta antibody on FFPE thymus with citrate retrieval but of course you will miss gamma/delta rearrangements but maybe that is not a concern. I've not tried the anti gamma/delta Ab on paraffin (only flow) but it might work. Perhaps others have tried. Also CD25 (low-affinity IL-2R) I've not had luck with on FFPE but maybe others have. Have had nice results with TdT (nuclear enzyme involved in TCR rearrangement) rabbit polyclonal on rat thymus FFPE. There are a few others, depends on the specificity and design of your model and exactly what you are looking for. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Kristen Broomall" > Good Morning all, I was wondering if anyone had suggestions on an antibody > to detect T cells (only, no B cells) in formalin fixed, paraffin embedded > rat thymus and spleen. I have a Pan T Cell marker that is working well in > the spleen, but so-so in the thymus, so I think that it is only staining the > mature T cells, and not immature cells (which of course, is what we also > need to see). I've been reading up alot on T cells and trying to figure this > out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear our > deadline for getting staining done is shorter than the time I have to > educate myself properly! > > Any suggestions would be greatly appreciated! > > > Happy Holidays, > Kristen Broomall, HT (ASCP) > histotechkb@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan <@t> mail.holyname.org Wed Dec 19 12:04:46 2007 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Wed Dec 19 12:05:05 2007 Subject: [Histonet] NJ STATE REGULATIONS AS TO HOW LONG TO RETAIN PARAFFIN BLOCKS AND GLASS SLIDES Message-ID: Hi NJ histonetters, How long do the slides and blocks needs to be retained in the State of NJ. Thanks in advance for your response. Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck, NJ 07666 From mwich <@t> 7thwavelabs.com Wed Dec 19 12:24:19 2007 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Dec 19 12:23:43 2007 Subject: [Histonet] decalcifiers and Safranin O staining Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486B6E@7THWAVE-SERVER.7thwave.local> Can anyone explain the chemistry behind decalcifying agents and the Safranin O procedure?...Specifically, why does using Immunocal (formic acid) as a decalcifier yield beautiful Safranin O staining while RDO (HCL) almost totally eliminates staining? Does HCL do something to the proteoglycans that formic acid does not? ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From b-frederick <@t> northwestern.edu Wed Dec 19 13:27:27 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Dec 19 13:27:48 2007 Subject: [Histonet] NJ STATE REGULATIONS AS TO HOW LONG TO RETAIN PARAFFIN BLOCKS AND GLASS SLIDES In-Reply-To: Message-ID: <000001c84275$34278070$d00f7ca5@lurie.northwestern.edu> CAP regulations state minimum retention guidelines. Some states and institutions go over and beyond those guidelines. You can view them on the CAP website. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Wednesday, December 19, 2007 12:05 PM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] NJ STATE REGULATIONS AS TO HOW LONG TO RETAIN PARAFFIN BLOCKS AND GLASS SLIDES Hi NJ histonetters, How long do the slides and blocks needs to be retained in the State of NJ. Thanks in advance for your response. Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck, NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Dec 19 13:44:26 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Dec 19 13:44:43 2007 Subject: AW: [Histonet] decalcifiers and Safranin O staining In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486B6E@7THWAVE-SERVER.7thwave.local> Message-ID: <000101c84277$910c8f40$eeeea8c0@dielangs.at> HCl has a acid-constant of -6 (strong acid) and formic acid has 3,75 (medium strong acid). Strong acids saponificate amidgroups resulting in an acid-group, deamination. Aminogroups are the reactionpartners of the collagen-stains. Therefore they can't bind to a deaminated collagen. Another function of a strong acid is to break down the peptidlinkage and generally denaturate a protein. The three-dimensional configuration of collagen could also influence the staining affinity. Doesn't Immunocal consist of formic acid and formaldehyd? I consider, that the deamination-effect and the break down appear more on underfixed specimen; and that formic acid isn't strong enough to disturb the collagen-configuration in the same manner. My thoughts about it, but no academic evidence. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Michele Wich Gesendet: Mittwoch, 19. Dezember 2007 19:24 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] decalcifiers and Safranin O staining Can anyone explain the chemistry behind decalcifying agents and the Safranin O procedure?...Specifically, why does using Immunocal (formic acid) as a decalcifier yield beautiful Safranin O staining while RDO (HCL) almost totally eliminates staining? Does HCL do something to the proteoglycans that formic acid does not? ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fong <@t> zoology.ubc.ca Wed Dec 19 15:24:31 2007 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Wed Dec 19 15:25:23 2007 Subject: [Histonet] rat perfusion In-Reply-To: <47693FA3.3070402@umdnj.edu> References: <000801c841f8$49bee040$d995c5d8@NEIL> <47693FA3.3070402@umdnj.edu> Message-ID: <47698C0F.2060009@zoology.ubc.ca> Hi Neil, I agree with Geoff. You don't need that much saline for the flush and it would be optimal to get the fix in ASAP. Usually I find ~80 ml is more than sufficient to flush and and if the heart was still beating when the perfusion begins, that is more than sufficient. I have also used room temperature saline, followed by 300-400ml 4% PFA also at room temperature. In most perfusion I would see a dance but not all. I would also ask the students to check that position of the needle is still correct, ie in the ascending aorta shortly after the start perfusing with saline, I have found from personal experience, that sometimes the needle moves while they clamp it in place. Another question: you are using netwells for the rinses but what about the incubations? Are you transferring sections from incubation wells to netwells? That can also be a point of damage. Just make sure they are really careful with the transferring if you are. I have seen beautiful sections get torn up from transferring and also sections from the same rat, handled by two different individuals come out looking very different. Have you tried staining a slice immediately after vibratome sectioning without immunohistochemical processing to see if the morphology is ok? Just some thoughts ... good luck with trouble shooting. Angelina > Hi Neil: > > Your first problem is cold perfusate.Why?? All this does is > constrict the blood vessels and imped fixation. The concept that cold > fixative slows down autolysis and improves fixation is a myth What > really happens is that the reaction of the fix with the tissue is > slowed. Yes, there are some instances when cold fix is better, but > this is not one of them. > There is no need to use so much saline washout; you are using well > over 10X the circulating blood volume. What you are doing is waiting 5 > to 10 minutes to begin fixation! You will never wash out all of the > blood, it is not necessary so don't try. Less than 100 ml is plenty. > As soon as the nose blanches,switch to fix. Room temperature fix, not > cold! > Use a 15 or 16 gauge needle, the others are too small to achieve > adequate flow. When you clamp the heart you may be impeding the flow > of fixative due to misplaced clamp, try holding the needle in place > and see if that improves your results. > I have had perfusions that looked "good" but gave mediocre > morphology and perfusions that looked "bad" with great morphology. I > would expect the 'dance' ~90% of the time. > I would slice the brain for post fixation, unless you must cut > through the whole brain. > I think the tearing problem is due to inadequate fixation. The > steps above should fix (ha!) it. > > Geoff > > Neil Fournier wrote: >> I have decided that our lab needs to revise our current protocol for >> rat intracardiac perfusion b/c the quality of perfused tissue has >> been reduced significantly over the last few months. I also thought >> it would be best to call upon the significant expertise of many of >> the histonet members. I hope that you might have some important >> suggestions and comments for us. I also apologize a priori for my >> naivety on this subject. >> >> Our protocol is as follows (for an adult rat typically 300 to 500 g): >> >> 1) Animals euthanized with a high dose of Euthanyl. >> 2) Perfuse cold 0.9% physiological saline (on ice). Using a perfusion >> pump with an 17 or 18 gauge blunted needle inserted into the >> ascending aorta. (I used to use 15 or 16 gauge needles) The heart is >> clamped to secure the needle tightly in place and the right atrium >> cut. Typically we perfuse between 300 and 500 ml over a five to ten >> minute period. >> 3) Perfuse cold 4% paraformaldehyde (on ice). (e.g., 40 g of >> paraformaldehyde dissolved in 800 ml warm PBS, filtered, cooled, and >> pH 7.4 with solution toped to a final volume of 1000 ml. All >> solutions are made up fresh, usually the day before or at least a few >> days before perfusion is required. Solutions are discarded if older >> than one week). The flow rate is 300 and 500 ml over a 10 to 15 >> minute period. >> *** I have noticed from time to time that when some students are >> perfusing some of the animals do not exhibit the typical spontaneous >> movements during fixation. However, sometimes the body will still >> appear rigid and fixed despite not showing these typical movement >> responses. I am uncertain if the classic "formalin dance" has to be a >> characteristic of a good whole body perfusion and was wondering what >> other people's take on this situation is. >> 4) Brains are removed and postfixed for 48 hrs before being sectioned >> on a Vibrating microtome at 50 microns. Tissue sections are usually >> stored in a ethylene glycol based cryoprotectant solution at a >> temperature between 20 and 25 degree C using a protocol identical to >> Gloria Hoffman's procedure (Peptides 1986, 7(1): 155-159.) >> Before I developed significant allergies, which makes my contact with >> the laboratory animals now at a minimal (poetic justice), I never had >> the same degree of problems that we seem to be observing lately. >> Although sectioning is not problematic with this tissue, the quality >> of tissue after IHC staining is generally poor (i.e., significant >> tearing of the tissue throughout etc.). Tissue is stained using the >> free-floating method and tissue is transferred from rinse to rinse >> using Netwells. I realize that this method in itself can contribute >> to some damage. I am not completely sure what the problem is but the >> consistency of this problem across animals and multiple experiments >> suggests to me one possibility is the actual perfusion procedure and >> that tissue integrity is minimal. >> >> Any suggestions and comments are welcomed. lastly, I was hoping that >> someone might know of a good book that discusses perfusion techniques >> in greater detail. Hopefully with some pictures as this is the often >> the best way to teach students (including myself). >> Thanks for the help, >> >> N >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5990 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From Kathy.Bucknell <@t> leica-microsystems.com Wed Dec 19 15:33:40 2007 From: Kathy.Bucknell <@t> leica-microsystems.com (Kathy.Bucknell@leica-microsystems.com) Date: Wed Dec 19 15:33:59 2007 Subject: [Histonet] Bucknell, Kathy is out of the office. Message-ID: I will be out of the office starting 12/19/2007 and will not return until 12/21/2007. I am at the Northbrook warehouse during this time and will have limited access to my emails. If you need immediate assistance, please contact me on my cell. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mtarango <@t> nvcancer.org Wed Dec 19 16:45:10 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Dec 19 16:45:32 2007 Subject: [Histonet] Rat T Cell Marker In-Reply-To: <667c97ab0712190603n3a0320fcpa9a5aff770ff4239@mail.gmail.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48C92@NVCIEXCH02.NVCI.org> SATB1 is a good nuclear marker for T-cells. I've only used it on human tissue though. I bet you can find an anti- rat SATB1. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Broomall Sent: Wednesday, December 19, 2007 6:03 AM To: Histonet Subject: [Histonet] Rat T Cell Marker Good Morning all, I was wondering if anyone had suggestions on an antibody to detect T cells (only, no B cells) in formalin fixed, paraffin embedded rat thymus and spleen. I have a Pan T Cell marker that is working well in the spleen, but so-so in the thymus, so I think that it is only staining the mature T cells, and not immature cells (which of course, is what we also need to see). I've been reading up alot on T cells and trying to figure this out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear our deadline for getting staining done is shorter than the time I have to educate myself properly! Any suggestions would be greatly appreciated! Happy Holidays, Kristen Broomall, HT (ASCP) histotechkb@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From koellingr <@t> comcast.net Thu Dec 20 00:44:35 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Dec 20 00:44:49 2007 Subject: [Histonet] Rat T Cell Marker Message-ID: <122020070644.20547.476A0F53000069F80000504322070245539D09020704040A0105@comcast.net> Kristen, To me something like CD3 is the way to go if I understand your project as you described it. Something like SATB1, which I've never used by IHC but know something about, seems like overkill or using a good (but incorrect) tool for the job. SATB1 (special AT-rich binding protein 1) I'm sure works well in Marks hands and others. But it is a not well characterized molecule being a loop-folding domain transcriptional regulator and while true found "mainly" in thymocytes, there are certainly indications of its presence in myeloid and erythroid precursors as well as developing mouse CNS. And it can be upregulated and down-regulated. And probably Marks statement that there might be anti-rat SATB1 (and SATB2) antibodies around is true, but might not be so easy to find or use. Compare that to the absolutely, precisely characterized and described gamma/delta/epsilon/zeta/eta CD3 TCR complex. It has been studied in minute detail all over the world, its presence or absence in cell types is documented and redocumented countless times. The number I've heard is about 10,000 copies per T-cell and literally defines that cell. If you wanted to "mark" T-cells in flow, frozen or paraffin I think 99% of labs would use CD3 at some point whether in periphery or thymus. Well characterized and protocoled anti-rat CD3 IHC antibodies exist in multiple clones and from multiple vendors for rat IHC. I like BD Pharmingen but just my opinion. Without ever having stained for SATB1 in rat, I believe what was said and guess yes you could do it, the question is, is that the easiest and most efficient way to get to your answer? Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Kristen Broomall" > Good Morning all, I was wondering if anyone had suggestions on an antibody > to detect T cells (only, no B cells) in formalin fixed, paraffin embedded > rat thymus and spleen. I have a Pan T Cell marker that is working well in > the spleen, but so-so in the thymus, so I think that it is only staining the > mature T cells, and not immature cells (which of course, is what we also > need to see). I've been reading up alot on T cells and trying to figure this > out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear our > deadline for getting staining done is shorter than the time I have to > educate myself properly! > > Any suggestions would be greatly appreciated! > > > Happy Holidays, > Kristen Broomall, HT (ASCP) > histotechkb@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Thu Dec 20 04:12:37 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Dec 20 04:13:27 2007 Subject: [Histonet] Rat T Cell Marker In-Reply-To: <122020070644.20547.476A0F53000069F80000504322070245539D09020704040A0105@comcast.net> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48C93@NVCIEXCH02.NVCI.org> Kristen & Ray, I still say SATB1 is the way to go. Kristen wants to stain T-cells that don't express CD3 yet. She'll need a T-cell marker that is expressed at the earliest point possible (to include all T-cells, even CD3-negative T-cells). Nearly every marker has some overlap with another lineage. She probably has already tried CD3, that being the most obvious and probably the first thing anyone would think of, when trying to stain a T-cell (but it ain't working out and she wants to know of other options). I really doubt she's working in a clinical rat lab. She's doing research; but even clinical labs end up using research antibodies. I've used SATB1 on human and mouse and it works great once your protocol is optimized. Take it from someone who's actually used it. How would you tell the difference between something like ALK-negative anaplastic large T-cell lymphoma and Hodgkin's lymphoma if the morphology is similar and the tumor cells don't express mature B or T-cell antigens? You have to go for something that is expressed at an earlier stage. I'd chose PAX5 (B-cells) and SATB1 (T-cells) to be able to figure it out. CD3 is useless in such a situation. This is research; don't be afraid to try something new to get you closer to your answer. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, December 19, 2007 10:45 PM To: Kristen Broomall; Histonet Subject: Re: [Histonet] Rat T Cell Marker Kristen, To me something like CD3 is the way to go if I understand your project as you described it. Something like SATB1, which I've never used by IHC but know something about, seems like overkill or using a good (but incorrect) tool for the job. SATB1 (special AT-rich binding protein 1) I'm sure works well in Marks hands and others. But it is a not well characterized molecule being a loop-folding domain transcriptional regulator and while true found "mainly" in thymocytes, there are certainly indications of its presence in myeloid and erythroid precursors as well as developing mouse CNS. And it can be upregulated and down-regulated. And probably Marks statement that there might be anti-rat SATB1 (and SATB2) antibodies around is true, but might not be so easy to find or use. Compare that to the absolutely, precisely characterized and described gamma/delta/epsilon/zeta/eta CD3 TCR complex. It has been studied in minute detail all over the world, its presence or absence in cell types is documented and redocumented countless times. The number I've heard is about 10,000 copies per T-cell and literally defines that cell. If you wanted to "mark" T-cells in flow, frozen or paraffin I think 99% of labs would use CD3 at some point whether in periphery or thymus. Well characterized and protocoled anti-rat CD3 IHC antibodies exist in multiple clones and from multiple vendors for rat IHC. I like BD Pharmingen but just my opinion. Without ever having stained for SATB1 in rat, I believe what was said and guess yes you could do it, the question is, is that the easiest and most efficient way to get to your answer? Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Kristen Broomall" > Good Morning all, I was wondering if anyone had suggestions on an antibody > to detect T cells (only, no B cells) in formalin fixed, paraffin embedded > rat thymus and spleen. I have a Pan T Cell marker that is working well in > the spleen, but so-so in the thymus, so I think that it is only staining the > mature T cells, and not immature cells (which of course, is what we also > need to see). I've been reading up alot on T cells and trying to figure this > out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear our > deadline for getting staining done is shorter than the time I have to > educate myself properly! > > Any suggestions would be greatly appreciated! > > > Happy Holidays, > Kristen Broomall, HT (ASCP) > histotechkb@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From joetworek <@t> yahoo.com Thu Dec 20 07:47:34 2007 From: joetworek <@t> yahoo.com (Joseph Tworek) Date: Thu Dec 20 07:47:50 2007 Subject: [Histonet] Dried out prostater cores Message-ID: <221466.35028.qm@web60814.mail.yahoo.com> Some of our prostate core biopsies are dried out to tthe poin that they resemble beef jerky under the micrscope. We have not been able to spot a trend. The biopsies involved come from various doctor's offices; all are received in formalin, some on on paper some are not (no difference); they are put back into formalin immediately after grossing; are run on different Tissue Tek VIP processors (temp logs withnin range); and are embedded first to reduce time in hot parrafin. We are still having problems. Anyone have any ideas? Thanks From Lynne.Bell <@t> hitchcock.org Thu Dec 20 08:18:33 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu Dec 20 08:18:44 2007 Subject: [Histonet] Dried out prostater cores In-Reply-To: <221466.35028.qm@web60814.mail.yahoo.com> Message-ID: We had this problem also. Do you use blue sponges in your cassettes? Our remedy was to keep the blue sponges in water and use them wet. Our problems were solved!! Happy Holidays, Lynne Lynne Bell, HT (ASCP) Department of Pathology Central Vermont Hospital 130 Fisher Road Barre, VT 05641 802-371-4923 From koellingr <@t> comcast.net Thu Dec 20 09:21:13 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Dec 20 09:21:31 2007 Subject: [Histonet] Rat T Cell Marker Message-ID: <122020071521.10215.476A886900061161000027E722007481849D09020704040A0105@comcast.net> Mark and Kristen, Hi, sorry, I didn't realize the target was T-cells expressing no CD3 although I'm not sure what that cell even is unless a T-cell precursor. Thymocytes are derived from a pool of CD3-/CD4-/CD8- T-cell precursors that have transited to the thymus. I didn't realize she wanted to see these also. But soon after, and in the vast majority of lymphocytes in thymus, they do express CD3 (and TCR). Otherwise they can not possibly go through positive and negative selection or anergy to become card carrying T-cells. So yes there are CD3- Tcell precursors in thymus (I guess) and so do see them by SATB1. I guess I do have to not be afraid to try something new and look into this research immunology and histology thing some day. Not exactly sure how diagnostics on transformed cells fits into the topic but I guess I misunderstood what Kristen was looking for. Thanks for the new information. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Tarango, Mark" > Kristen & Ray, > > I still say SATB1 is the way to go. Kristen wants to stain T-cells that > don't express CD3 yet. She'll need a T-cell marker that is expressed at > the earliest point possible (to include all T-cells, even CD3-negative > T-cells). > > Nearly every marker has some overlap with another lineage. She probably > has already tried CD3, that being the most obvious and probably the > first thing anyone would think of, when trying to stain a T-cell (but it > ain't working out and she wants to know of other options). I really > doubt she's working in a clinical rat lab. She's doing research; but > even clinical labs end up using research antibodies. > > I've used SATB1 on human and mouse and it works great once your protocol > is optimized. Take it from someone who's actually used it. > > How would you tell the difference between something like ALK-negative > anaplastic large T-cell lymphoma and Hodgkin's lymphoma if the > morphology is similar and the tumor cells don't express mature B or > T-cell antigens? You have to go for something that is expressed at an > earlier stage. I'd chose PAX5 (B-cells) and SATB1 (T-cells) to be able > to figure it out. CD3 is useless in such a situation. > > This is research; don't be afraid to try something new to get you closer > to your answer. > > Mark Adam Tarango HT(ASCP) > Histology & IHC Supervisor > Nevada Cancer Institute > One Breakthrough Way > Las Vegas, NV 89135 > Direct Line (702) 822-5112 > Treo (702) 759-9229 > Fax (702) 939-7663 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > koellingr@comcast.net > Sent: Wednesday, December 19, 2007 10:45 PM > To: Kristen Broomall; Histonet > Subject: Re: [Histonet] Rat T Cell Marker > > Kristen, > To me something like CD3 is the way to go if I understand your project > as you described it. Something like SATB1, which I've never used by IHC > but know something about, seems like overkill or using a good (but > incorrect) tool for the job. SATB1 (special AT-rich binding protein 1) > I'm sure works well in Marks hands and others. But it is a not well > characterized molecule being a loop-folding domain transcriptional > regulator and while true found "mainly" in thymocytes, there are > certainly indications of its presence in myeloid and erythroid > precursors as well as developing mouse CNS. And it can be upregulated > and down-regulated. And probably Marks statement that there might be > anti-rat SATB1 (and SATB2) antibodies around is true, but might not be > so easy to find or use. Compare that to the absolutely, precisely > characterized and described gamma/delta/epsilon/zeta/eta CD3 TCR > complex. It has been studied in minute detail all over the world, its > presence or absence in cell types > is documented and redocumented countless times. The number I've heard > is about 10,000 copies per T-cell and literally defines that cell. If > you wanted to "mark" T-cells in flow, frozen or paraffin I think 99% of > labs would use CD3 at some point whether in periphery or thymus. Well > characterized and protocoled anti-rat CD3 IHC antibodies exist in > multiple clones and from multiple vendors for rat IHC. I like BD > Pharmingen but just my opinion. Without ever having stained for SATB1 in > rat, I believe what was said and guess yes you could do it, the question > is, is that the easiest and most efficient way to get to your answer? > > Ray Koelling > PhenoPath Labs > Seattle, WA > > -------------- Original message -------------- > From: "Kristen Broomall" > > > Good Morning all, I was wondering if anyone had suggestions on an > antibody > > to detect T cells (only, no B cells) in formalin fixed, paraffin > embedded > > rat thymus and spleen. I have a Pan T Cell marker that is working well > in > > the spleen, but so-so in the thymus, so I think that it is only > staining the > > mature T cells, and not immature cells (which of course, is what we > also > > need to see). I've been reading up alot on T cells and trying to > figure this > > out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear > our > > deadline for getting staining done is shorter than the time I have to > > educate myself properly! > > > > Any suggestions would be greatly appreciated! > > > > > > Happy Holidays, > > Kristen Broomall, HT (ASCP) > > histotechkb@gmail.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "EMF " made the following annotations. > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended > recipient(s) and may contain confidential, proprietary, > and/or privileged information protected by law. If you are > not the intended recipient, you may not use, copy, or > distribute this e-mail message or its attachments. If you > believe you have received this e-mail message in error, > please contact the sender by reply e-mail and destroy all > copies of the original message > ============================================================================== > From Herrick.James <@t> mayo.edu Thu Dec 20 11:27:33 2007 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Thu Dec 20 11:27:47 2007 Subject: [Histonet] Immunostaining on plastic embedded tissue Message-ID: <572057D3BDD52A46BD05BC6DA50686110CAF21@MSGEBE22.mfad.mfroot.org> Hi, I am trying immunostaining (for actin and Von Willebrand Factor) on MMA resin embedded arterial tissue with little success at this point. I sectioned at 5um and 10 um thicknesses and deplastifyed with a couple of 3 minute rinses of 2-Methoxyethyl acetate, followed by a quick (approx. 20-30 sec.) rinse in chloroform. My sections have been sitting out at room temp (following deplastification) for about 2 weeks before I begin the immuno staining -- should they be stored in the refrigerator or freezer? The sections look great and the plastic appears to be completely dissolved, but I am thinking I may need to treat longer. Also, I may try some type of antigen retrieval. Does anyone have experience with this type of staining and if so, could you please give me some tips on what I may need to change. Thanks for your help. Jim From bitesizellama <@t> gmail.com Thu Dec 20 11:32:29 2007 From: bitesizellama <@t> gmail.com (Mauricio Avigdor) Date: Thu Dec 20 11:32:45 2007 Subject: [Histonet] Osmometer Message-ID: <1b2831cd0712200932v3790bf5bofda917c9387ea587@mail.gmail.com> Dear listers: Is there a way to measure osmolality that does not involve an osmometer? I asked to borrow an osmometer from another lab, and was told that they no longer use these, as they have moved on to "more modern techniques". I am really curious to know what these techniques could be. From cmiller <@t> physlab.com Thu Dec 20 11:42:28 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Dec 20 11:42:50 2007 Subject: [Histonet] CD68 Message-ID: <000901c8432f$b0d48220$3402a8c0@plab.local> I am very new to IHC and I need some advice. I have a Nexus IHC from Ventana and for some reason the tonsil-control for CD68 KP-1 isn't working.The patient slide works but my control tissue doesn't. the tonsil is from a 2 yr old. I was told that it should work. Any suggestions?? Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Thu Dec 20 11:51:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 20 11:51:38 2007 Subject: [Histonet] Osmometer In-Reply-To: <1b2831cd0712200932v3790bf5bofda917c9387ea587@mail.gmail.com> Message-ID: <618733.10337.qm@web61213.mail.yahoo.com> Why don't you just ask them which is the "modern technique" they use? They should know. Ren? J. Mauricio Avigdor wrote: Dear listers: Is there a way to measure osmolality that does not involve an osmometer? I asked to borrow an osmometer from another lab, and was told that they no longer use these, as they have moved on to "more modern techniques". I am really curious to know what these techniques could be. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Thu Dec 20 11:58:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 20 11:59:03 2007 Subject: [Histonet] CD68 In-Reply-To: <000901c8432f$b0d48220$3402a8c0@plab.local> Message-ID: <684979.99598.qm@web61212.mail.yahoo.com> IF you have both control and case running simultaneously and there is no problem with the instrument (like malfunctioning over one slide and not over the other, which is very unlikely) your problem is with your control. I always used tonsils coming from surgey and ususally older than 2 yo patients. I used Dako IgMo Ab at 1:1000 with pH6 HIER Try a new control tissue (fresh sections if at all possible). Ren? J. Cheri Miller wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From michael.owen <@t> fda.hhs.gov Thu Dec 20 12:04:17 2007 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Thu Dec 20 12:05:03 2007 Subject: [Histonet] IHC Technologist Position at PhenoPath (Seattle, WA) Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF0422035E@FMD3VS022.fda.gov> Immunohistochemistry (IHC) Technologist craigslist Seattle-Tacoma / jobs / biotech and science http://seattle.craigslist.org http://seattle.craigslist.org/see/sci/514227920.html ------------------------------------------------------------------------ -------- Reply to: jobs@phenopath.com Date: 2007-12-19, 9:46AM PST PhenoPath Laboratories, a national pathology reference laboratory, has an opportunity in the clinical immunohistochemistry division for a full-time IHC technologist, day shift. We are in a state-of-the-art facility located in Seattle, WA. JOB DESCRIPTION: Responsibilities may include performing immunohistochemistry, and immunofluorescence on patient samples and tissue sectioning (paraffin and frozen). Participation in the development of new tests or technologies, as well as participation in clinical research projects is also included. Quality control, safety, and preventive maintenance procedures and documentation are required elements of this position as well. REQUIRED SKILLS/EXPERIENCE: Strong preference given to ASCP certified (or certification-eligible) laboratory techs. Trained laboratory techs of any discipline are encouraged to apply (histotech, med tech, cytotech). PhenoPath Laboratories is committed to hiring the best person for the job. PhenoPath Laboratories offers a competitive compensation and benefits package, including 401k. TO APPLY, PLEASE E-MAIL OR FAX COMPLETED APPLICATION FOR EMPLOYMENT AND RESUME TO: Paul Moore, Assistant to the Director of Human Resources PhenoPath Laboratories 551 N. 34th St., Suite 100 Seattle, WA 98103 Fax: 206 374-9009 E-mail: jobs@phenopath.com (Preferred method of application) No phone calls about this job, please. Location: Seattle, WA Compensation: DOE Principals only. Recruiters, please don't contact this job poster. Please, no phone calls about this job! Please do not contact job poster about other services, products or commercial interests. PostingID: 514227920 Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From rchiovetti <@t> yahoo.com Thu Dec 20 12:11:16 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Thu Dec 20 12:11:35 2007 Subject: [Histonet] Osmometer Message-ID: <756859.64500.qm@web58911.mail.re1.yahoo.com> Hi Mauricio, Well, that's interesting. The "more modern techniques" still require an instrument to measure osmolality! Maybe you need to ask someone else in the laboratory... There are two basic ways to measure osmolality: vapor pressure and freezing point depression. In the vapor pressure instruments, you soak a small filter paper circle with the solution, put it in a sealed chamber and measure the amount of heat that is required to vaporize the solution and form an amount of pressure in the chamber. The freezing point depression machines usually require a little more solution. It's put in a small chamber, the chamber is chilled, and the temperature is measured at which the solution freezes. There is sometimes a small vibrating probe in the solution to agitate it while it's cooling down. At least these are the two old standby methods. There are probably more freezing point depression osmometers in use than vapor pressure osmometers, but they have both been around for many years! Maybe you should ask someone else in another lab? Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments See What's New on Our Website! Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- I asked to borrow an osmometer from another lab, and was told that they no longer use these, as they have moved on to "more modern techniques". I am really curious to know what these techniques could be. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From majid.ghoddusi <@t> gmail.com Thu Dec 20 12:15:49 2007 From: majid.ghoddusi <@t> gmail.com (Majid ghoddusi) Date: Thu Dec 20 12:16:05 2007 Subject: [Histonet] CD68 In-Reply-To: <684979.99598.qm@web61212.mail.yahoo.com> References: <000901c8432f$b0d48220$3402a8c0@plab.local> <684979.99598.qm@web61212.mail.yahoo.com> Message-ID: While on CD 68 subject, I have been told that this does not work on mouse tissue. In other words it is not a good IHC marker for mouse macrophages. I would appreciate advice from people who have successfuly tried this on mouse tissue. Thanks, Majid *Majid Ghoddusi, DVM, PhD* *Veterinary Pathologist* *Comparative Biosciences, Inc.* *786 Lucerne Drive, * *Sunnyvale, CA 94085* *http://www.compbio.com/* *Ph: 408-738-9265* *Fax: 408-738-9278* On Dec 20, 2007 9:58 AM, Rene J Buesa wrote: > IF you have both control and case running simultaneously and there is no > problem with the instrument (like malfunctioning over one slide and not over > the other, which is very unlikely) your problem is with your control. > I always used tonsils coming from surgey and ususally older than 2 yo > patients. > I used Dako IgMo Ab at 1:1000 with pH6 HIER > Try a new control tissue (fresh sections if at all possible). > Ren? J. > > Cheri Miller wrote: > > > > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From arnie.jimenez <@t> vel-lab.com Thu Dec 20 12:11:50 2007 From: arnie.jimenez <@t> vel-lab.com (arnie.jimenez@vel-lab.com) Date: Thu Dec 20 12:23:01 2007 Subject: [Histonet] Happy Holidays! Message-ID: <20071220181150.19040.qmail@ux-vhost05.dllstx2.theplanet.com> This is an automated response. Thank you for contacting Vel-Lab Research. We are shutdown for the holidays. We use this time to give our employees a well deserved break, we also take the opportunity to thouroghly clean our facilities and calibrate all our equipment. Your project is our top priority and we will return to it as soon as possible. I will personally return to the lab Dec. 27th and will attend to any urgent issues then. The full lab will be back the first week of January. Thank you for your understanding. Happy Holidays from everyone at Vel-Lab Arnie Jimenez Owner Vel-Lab Research From jwray78 <@t> gmail.com Thu Dec 20 12:27:35 2007 From: jwray78 <@t> gmail.com (Josh Wray) Date: Thu Dec 20 12:27:49 2007 Subject: [Histonet] RE: CD68 Message-ID: <883927cf0712201027u1dc19f6o998092564f7eebec@mail.gmail.com> We too had a problem with tonsil for a CD68 control. We decided to run a lymph node as the QC. It worked out much better. Josh Wray HT(ASCP) Ameripath Indianapolis From MITCHELLJA <@t> email.chop.edu Thu Dec 20 12:43:19 2007 From: MITCHELLJA <@t> email.chop.edu (Janice Mitchell) Date: Thu Dec 20 12:44:17 2007 Subject: [Histonet] MICROWAVES Message-ID: Cap requests "microwave devices be periodically monitored for reproducibility" what is considered periodically? Thanks, Janice From CIngles <@t> uwhealth.org Thu Dec 20 12:59:10 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Dec 20 12:59:24 2007 Subject: [Histonet] Off Topic: NO SANTA CLAUS References: <004901c84310$47c91f80$7cafbe44@your03667082de> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200B7@uwhis-xchng3.uwhis.hosp.wisc.edu> Sorry if you are not Christian. You can delete, but I think Santa just has lots of different names around the world. Everyone - remember what you have that never cost anything. Merry Christmas greetings and warm wishes to all. Claire Ingles lifelong Santa's helper I remember my first Christmas adventure with Grandma. I was just a kid. I remember tearing across town on my bike to visit her on the day my big sister dropped the bomb: "There is no Santa Claus," she jeered. "Even dummies know that!" My Grandma was not the gushy kind, never had been. I fled to her that day because I knew she would be straight with me. I knew Grandma always told the truth, and I knew that the truth always went down a whole lot easier when swallowed with one of her worl d-famous cinnamon buns. I knew they were world-famous, because Grandma said so. It had to be true. Grandma was home, and the buns were still warm. Between bites, I told her everything. She was ready for me. "No Santa Claus! !" she snorted. "Ridiculous! " Don't believe it. That rumor has been going around for years, and it makes me mad, plain mad.' Now, put on your coat, and let's go." "Go? Go where, Grandma?" I asked. I hadn't even finished my second world-famous, cinnamon bun. "Where" turned out to be Kerby's General Store, the one store in town that had a little bit of just about everything. As we walked through its doors, Grandma handed me ten dollars. That was a bundle in those days. "Take this money," she said, "and buy something for someone who needs it. I'll wait for you in the car." Then she turned and walked out of Kerby's. I was only eight years old. I'd often gone shopping with my mother, but never had I shopped for anything all by myself. The store seemed big and crowded, full of people scrambling to finish their Christmas shopping. For a few moments I just stood there, confused, clutching that ten-dollar bill, wondering what to buy, and who on earth to buy it for. I thought of everybody I knew: my family, my friends, my neighbors, the kids at school, the people who went to my church. I was just about thought out, when I suddenly thought of Bobby Decker. He was a kid with bad breath and messy hair, and he sat right behind me in Mrs. Pollock's grade-two class. Bobby Decker didn't have a coat. I knew that because he never went out for recess during the winter. His mother always wrote a note, telling the teacher that he had a cough, but all we kids knew that Bobby Decker didn't have a cough, and he didn't have a coat. I fingered the ten-dollar bill with growing excitement. I would buy Bobby Decker a coat! I settled on a red corduroy one that had a hood to it. It looked real warm, and he would like that. "Is this a Christmas present for someone?" the lady behind the counter asked kindly, as I laid my ten dollars down. "Yes," I replied shyly. "It's ... for Bobby." The nice lady smiled at me. I didn't get any change, but she put the coat in a bag and wished me a Merry Christmas. That evening, Grandma helped me wrap the coat in Christmas paper and ribbons (a little tag fell out of the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From Santa Claus", on a tag-- Grandma said that Santa always insisted on secrecy. Then she drove me over to Bobby Decker's house, explaining as we went that I was now and forever officially one of Santa's helpers. Grandma parked down the street from Bobby's house, and she and I crept noiselessly and hid in the bushes by his front walk. Then Gr andma gave me a nudge. "All right, Santa Claus," she whispered, "get going." I took a deep breath, dashed for his front door, and threw the present down on his step. I pounded his door and flew back to the safety of the bushes and Grandma. Together we waited breathlessly in the darkness for the front door to open. Finally it did, and there stood Bobby. Fifty years haven't dimmed the thrill of those moments spent shivering beside my Grandma, in Bobby Decker's bushes. That night, I realized that those awful rumors about Santa Claus were just what Grandma said they were: "Ridiculous". Santa was alive and well, and we were on his team. I still have the Bible, with the tag tucked inside: $19.95. -------------------------------------------------------------------- He who has no Christmas in his heart will never find Christmas under a tree. Have a wonderful holiday season. Merry Christmas and a Happy New Year!! From tjasper <@t> copc.net Thu Dec 20 14:07:49 2007 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Dec 20 14:08:03 2007 Subject: [Histonet] Off Topic: NO SANTA CLAUS References: <004901c84310$47c91f80$7cafbe44@your03667082de> <08A0A863637F1349BBFD83A96B27A50A1200B7@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <90354A475B420441B2A0396E5008D4965E201A@copc-sbs.COPC.local> Thanks Claire, totally awesome. Merry Christmas and Happy New Year from one of Santa helpers to another. Tom Jasper Bend, OR (by way of northern WI) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, December 20, 2007 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off Topic: NO SANTA CLAUS Sorry if you are not Christian. You can delete, but I think Santa just has lots of different names around the world. Everyone - remember what you have that never cost anything. Merry Christmas greetings and warm wishes to all. Claire Ingles lifelong Santa's helper I remember my first Christmas adventure with Grandma. I was just a kid. I remember tearing across town on my bike to visit her on the day my big sister dropped the bomb: "There is no Santa Claus," she jeered. "Even dummies know that!" My Grandma was not the gushy kind, never had been. I fled to her that day because I knew she would be straight with me. I knew Grandma always told the truth, and I knew that the truth always went down a whole lot easier when swallowed with one of her worl d-famous cinnamon buns. I knew they were world-famous, because Grandma said so. It had to be true. Grandma was home, and the buns were still warm. Between bites, I told her everything. She was ready for me. "No Santa Claus! !" she snorted. "Ridiculous! " Don't believe it. That rumor has been going around for years, and it makes me mad, plain mad.' Now, put on your coat, and let's go." "Go? Go where, Grandma?" I asked. I hadn't even finished my second world-famous, cinnamon bun. "Where" turned out to be Kerby's General Store, the one store in town that had a little bit of just about everything. As we walked through its doors, Grandma handed me ten dollars. That was a bundle in those days. "Take this money," she said, "and buy something for someone who needs it. I'll wait for you in the car." Then she turned and walked out of Kerby's. I was only eight years old. I'd often gone shopping with my mother, but never had I shopped for anything all by myself. The store seemed big and crowded, full of people scrambling to finish their Christmas shopping. For a few moments I just stood there, confused, clutching that ten-dollar bill, wondering what to buy, and who on earth to buy it for. I thought of everybody I knew: my family, my friends, my neighbors, the kids at school, the people who went to my church. I was just about thought out, when I suddenly thought of Bobby Decker. He was a kid with bad breath and messy hair, and he sat right behind me in Mrs. Pollock's grade-two class. Bobby Decker didn't have a coat. I knew that because he never went out for recess during the winter. His mother always wrote a note, telling the teacher that he had a cough, but all we kids knew that Bobby Decker didn't have a cough, and he didn't have a coat. I fingered the ten-dollar bill with growing excitement. I would buy Bobby Decker a coat! I settled on a red corduroy one that had a hood to it. It looked real warm, and he would like that. "Is this a Christmas present for someone?" the lady behind the counter asked kindly, as I laid my ten dollars down. "Yes," I replied shyly. "It's ... for Bobby." The nice lady smiled at me. I didn't get any change, but she put the coat in a bag and wished me a Merry Christmas. That evening, Grandma helped me wrap the coat in Christmas paper and ribbons (a little tag fell out of the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From Santa Claus", on a tag-- Grandma said that Santa always insisted on secrecy. Then she drove me over to Bobby Decker's house, explaining as we went that I was now and forever officially one of Santa's helpers. Grandma parked down the street from Bobby's house, and she and I crept noiselessly and hid in the bushes by his front walk. Then Gr andma gave me a nudge. "All right, Santa Claus," she whispered, "get going." I took a deep breath, dashed for his front door, and threw the present down on his step. I pounded his door and flew back to the safety of the bushes and Grandma. Together we waited breathlessly in the darkness for the front door to open. Finally it did, and there stood Bobby. Fifty years haven't dimmed the thrill of those moments spent shivering beside my Grandma, in Bobby Decker's bushes. That night, I realized that those awful rumors about Santa Claus were just what Grandma said they were: "Ridiculous". Santa was alive and well, and we were on his team. I still have the Bible, with the tag tucked inside: $19.95. -------------------------------------------------------------------- He who has no Christmas in his heart will never find Christmas under a tree. Have a wonderful holiday season. Merry Christmas and a Happy New Year!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsnwl <@t> neuro.hfh.edu Thu Dec 20 14:22:11 2007 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Thu Dec 20 14:36:17 2007 Subject: [Histonet] Off Topic: NO SANTA CLAUS Message-ID: Merry Christmas to you both and to all Santa's helpers, big and small! Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Jasper" tjasper@copc.net Date: Thu, 20 Dec 2007 16:09:31 -0500 To: "Ingles Claire" CIngles@uwhealth.org Subject: RE: [Histonet] Off Topic: NO SANTA CLAUS > Thanks Claire, totally awesome. Merry Christmas and Happy New Year from > one of Santa helpers to another. > Tom Jasper > Bend, OR (by way of northern WI) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Thursday, December 20, 2007 10:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Off Topic: NO SANTA CLAUS > > > Sorry if you are not Christian. You can delete, but I think Santa just > has lots of different names around the world. Everyone - remember what > you have that never cost anything. Merry Christmas greetings and warm > wishes to all. > > Claire Ingles > lifelong Santa's helper > > > > I remember my first Christmas > adventure with Grandma. > I was just a kid. > > I remember tearing across town > on my bike to visit her on the day my big sister dropped the bomb: > > "There is no Santa Claus," she > jeered. "Even dummies know that!" > > My Grandma was not the gushy > kind, never had been. > > I fled to her that day because I > knew she would be straight with me. I knew Grandma always told the > truth, and I knew that the truth always went down a whole lot easier > when swallowed with one of her worl d-famous cinnamon buns. > > I knew they were world-famous, > because Grandma said so. > It had to be true. > Grandma was home, and the buns > were still warm. > > Between bites, I told her > everything. She was ready for me. > "No Santa Claus! !" she snorted. > "Ridiculous! > " Don't believe it. That rumor > has been going around for years, and it makes me mad, plain mad.' > > Now, put on your coat, and let's > go." > "Go? Go where, Grandma?" I > asked. > I hadn't even finished my second > world-famous, cinnamon bun. > > "Where" turned out to be Kerby's > General Store, the one store in town that had a little bit of just about > everything. As we walked through its doors, Grandma handed me ten > dollars. That was a bundle in those days. > > "Take this money," she said, > "and buy something for someone who needs it. I'll wait for you in the > car." > > Then she turned and walked out > of Kerby's. I was only eight years old. I'd often gone shopping with my > mother, but never had I shopped for anything all by myself. > > The store seemed big and > crowded, full of people scrambling to finish their Christmas shopping. > For a few moments I just stood there, confused, clutching that > ten-dollar bill, wondering what to buy, and who on earth to buy it for. > > I thought of everybody I knew: > my family, my friends, my neighbors, the kids at school, the people who > went to my church. > > I was just about thought out, > when I suddenly thought of Bobby Decker. He was a kid with bad breath > and messy hair, and he sat right behind me in Mrs. Pollock's grade-two > class. Bobby Decker didn't have a coat. > > I knew that because he never > went out for recess during the winter. His mother always wrote a note, > telling the teacher that he had a cough, but all we kids knew that Bobby > Decker didn't have a cough, and he didn't have a coat. > > I fingered the ten-dollar bill > with growing excitement. I would buy Bobby Decker a coat! I settled on> a > red corduroy one that had a hood to it. It looked real warm, and he > would like that. > > "Is this a Christmas present for > someone?" the lady behind the counter asked kindly, as I laid my ten > dollars down. > > "Yes," I replied shyly. "It's > ... for Bobby." > > The nice lady smiled at me. I > didn't get any change, but she put the coat in a bag and wished me a > Merry Christmas. > > That evening, Grandma helped me > wrap the coat in Christmas paper and ribbons (a little tag fell out of > the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From > Santa Claus", on a tag-- Grandma said that Santa always insisted on > secrecy. > > Then she drove me over to Bobby > Decker's house, explaining as we went that I was now and forever > officially one of Santa's helpers. > > Grandma parked down the street > from Bobby's house, and she and I crept noiselessly and hid in the > bushes by his front walk. Then Gr andma gave me a nudge. "All right, > Santa Claus," she whispered, "get going." > I took a deep breath, dashed for > his front door, and threw the present down on his step. I pounded his > door and flew back to the safety of the bushes and Grandma. > > Together we waited breathlessly > in the darkness for the front door to open. Finally it did, and there > stood Bobby. Fifty years haven't dimmed the thrill of those moments > spent shivering beside my Grandma, in Bobby Decker's bushes. > > That night, I realized that > those awful rumors about Santa Claus were just what Grandma said they > were: "Ridiculous". Santa was alive and well, and we were on his team. > > I still have the Bible, with the > tag tucked inside: $19.95. > > > -------------------------------------------------------------------- > He who has no Christmas in his > heart will never find Christmas under a tree. > > Have a wonderful holiday season. > Merry Christmas and a Happy New Year!! > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From POWELL_SA <@t> Mercer.edu Thu Dec 20 14:52:58 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Dec 20 14:56:31 2007 Subject: [Histonet] Tissue Tek anti-roll device Message-ID: <01MP4ABAC5UE0012GH@Macon2.Mercer.edu> Hi Guys, I am reaching for straws, but I need to locate an anti-roll device for an older model Lab Tek, Tissue Tek cryostat. Mine finally was destroyed after ohhhhh, say 25 years. Need to replace it or rebuild it. Any help would be appreciated. Thanks Shirley Powell, HT(ASCP)HTL, QIHC Technical Director Histopathology Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 From PMcArdle <@t> ebsciences.com Thu Dec 20 14:57:17 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Thu Dec 20 14:57:36 2007 Subject: [Histonet] MICROWAVES In-Reply-To: References: Message-ID: <476AD72D.1080302@ebsciences.com> Hi: This response is from a microwave vendor, so you've been forewarned! :-) That said, it seems analogous ISO-9000 certification: each lab is allowed to make its own determination as to the meaning of "periodically;" the procedure should be documented and assigned AND (and here's the key part) adhered to and logged! I've seen some labs with very nicely thought out and documented procedures, who got written up because they didn't follow their own procedures. When CAP began their microwave leakage measurement requirement, it specified "at least" yearly, and EBS recommended quarterly. (Arbitrary? Maybe.) As for reproducibility, I feel that yearly is not enough, daily or weekly is probably excessive, but depending upon your lab's volume, monthly or quarterly should be sufficient, unless anything unusual is noted (blown fuse, etc.). Hope this helps! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Janice Mitchell wrote: > Cap requests "microwave devices be periodically monitored for > reproducibility" what is considered periodically? > Thanks, Janice > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Thu Dec 20 15:01:22 2007 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Thu Dec 20 15:01:21 2007 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 12/20/2007 and will not return until 12/24/2007. I will return Tuesday November 13 at 11 PM and will respond to your message when I return. Thanks, Jackie From JMacDonald <@t> mtsac.edu Thu Dec 20 15:09:33 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Dec 20 15:09:51 2007 Subject: [Histonet] Tissue Tek anti-roll device In-Reply-To: <01MP4ABAC5UE0012GH@Macon2.Mercer.edu> Message-ID: Try Sakura. That is where we bought ours, just a few of years ago. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Shirley Powell Sent by: histonet-bounces@lists.utsouthwestern.edu 12/20/2007 12:52 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tissue Tek anti-roll device Hi Guys, I am reaching for straws, but I need to locate an anti-roll device for an older model Lab Tek, Tissue Tek cryostat. Mine finally was destroyed after ohhhhh, say 25 years. Need to replace it or rebuild it. Any help would be appreciated. Thanks Shirley Powell, HT(ASCP)HTL, QIHC Technical Director Histopathology Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Thu Dec 20 15:24:32 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Dec 20 15:24:49 2007 Subject: [Histonet] Christmas Greetings, and thanks to... Message-ID: <8CA1165D8975232-D9C-6672@WEBMAIL-MB20.sysops.aol.com> I would like to join in wishing everyone a happy Christmas, this time from the "Heart of Dixie", ?and to thank Dr Margraf and others?for hosting the "Histonet". Thanks also to all the subscribers whose questions and answers help keep me informed.?Life is so interesting! Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From Gguerzon <@t> lifebridgehealth.org Thu Dec 20 15:27:38 2007 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Thu Dec 20 15:28:10 2007 Subject: [Histonet] IHC for VIAS Message-ID: We are having problem with tissue falling off the slides when we run the breast panel on the Ventana XT for examination with the VIAS instrument. The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and place them in 45 degree oven overnight and they still fall of. Any suggestions? Thanks. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From doug <@t> ppspath.com Thu Dec 20 15:46:07 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Dec 20 15:46:39 2007 Subject: SPAM-LOW: [Histonet] IHC for VIAS In-Reply-To: Message-ID: The first question that Ventana will ask is what brand of slides are you using? If you are using the "correct slides" do you use any adhesive in your water bath? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Thursday, December 20, 2007 4:28 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] IHC for VIAS We are having problem with tissue falling off the slides when we run the breast panel on the Ventana XT for examination with the VIAS instrument. The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and place them in 45 degree oven overnight and they still fall of. Any suggestions? Thanks. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Dec 20 15:55:34 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Dec 20 15:55:55 2007 Subject: [Histonet] MITF Message-ID: Does anyone have a good MITF protocol for the Ventana Benchmark XT? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Kathy.Abels <@t> sial.com Thu Dec 20 16:02:24 2007 From: Kathy.Abels <@t> sial.com (Kathy Abels) Date: Thu Dec 20 16:02:56 2007 Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. Message-ID: I will be out of the office starting 12/19/2007 and will not return until 01/02/2008. I will respond to your message when I return. For urgent matters, Please contact Sherry Chappell or Leigh Gaskill. This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From ploykasek <@t> phenopath.com Thu Dec 20 16:11:40 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Dec 20 16:11:56 2007 Subject: [Histonet] IHC for VIAS In-Reply-To: Message-ID: I would recommend some time in a 60-65 degree oven even if you do still want to leave them in a 37-45 degree oven overnight. That's just off the top of my head - busy day here. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Ronald.Houston <@t> nationwidechildrens.org Thu Dec 20 16:18:26 2007 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Dec 20 16:19:07 2007 Subject: [Histonet] IgA-FITC Message-ID: <979FF5962E234F45B06CF0DB7C1AABB214284CE9@chi2k3ms01.columbuschildrens.net> Here's a new one for me, but I'm sure someone out there has been approached about this (at least I hope so!) Has anyone attempted to demonstrate IgA in human breast milk for fluorescence photomicroscopy? Preliminary attempts light up like a Christmas tree................. Thanks and Merry Christmas Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org From richardkuzma <@t> michdiag.com Thu Dec 20 16:22:52 2007 From: richardkuzma <@t> michdiag.com (richardkuzma@michdiag.com) Date: Thu Dec 20 16:23:15 2007 Subject: [Histonet] Off topic: Free samples of chemiluminescent substrates Message-ID: <1928.1198189372@michdiag.com> Michigan Diagnostics Is offering free evaluation samples to the research co number, so we don Luminol based HRP substrate and dioxetane based AP substrate are what were We do have other substrates for sale, including chemilumi substrates for the detection of Beta-Glucosidase, Beta-Galactosidase and Be www.michdiag.c Sorry if you think this is too off topic. Please don't flame me too badly. Best Regards, Richard Kuzma Production Manager Michigan< Royal Oak, MI. 48073 USA richardkuzma@michdiag.com www.michdiag.com (248)435-4472 (248)435-4537 From Gguerzon <@t> lifebridgehealth.org Thu Dec 20 17:02:22 2007 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Thu Dec 20 17:03:42 2007 Subject: [Histonet] IHC for VIAS Message-ID: Thanks Patti, We have tried this method of 30 minutes in a 60degree oven and overnight at 37-40 degree oven - it gives us better results but still part of the tissue is falling off. Thanks. Godfrey >>> Patti Loykasek 12/20/2007 5:11 PM >>> I would recommend some time in a 60-65 degree oven even if you do still want to leave them in a 37-45 degree oven overnight. That's just off the top of my head - busy day here. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From annette_hall <@t> pa-ucl.com Thu Dec 20 17:09:28 2007 From: annette_hall <@t> pa-ucl.com (Annette Hall) Date: Thu Dec 20 17:03:47 2007 Subject: [Histonet] IHC for VIAS In-Reply-To: Message-ID: <71320B4EBC7C15419563EAFBFCD924651C7BD48A33@hades.pa-ucl.com> We had the same problem with our XT. We now put the slides at 60 degrees for 1-2 hours. It's not perfect but we retain most of the tissue. Annette J Hall, MT Histo Supervisor United Clinical Labs Dubuque, Ia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, December 20, 2007 4:12 PM To: Godfrey Guerzon; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC for VIAS I would recommend some time in a 60-65 degree oven even if you do still want to leave them in a 37-45 degree oven overnight. That's just off the top of my head - busy day here. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From arnie.jimenez <@t> vel-lab.com Thu Dec 20 16:58:36 2007 From: arnie.jimenez <@t> vel-lab.com (arnie.jimenez@vel-lab.com) Date: Thu Dec 20 17:09:51 2007 Subject: [Histonet] Happy Holidays! Message-ID: <20071220225836.1675.qmail@ux-vhost05.dllstx2.theplanet.com> This is an automated response. Thank you for contacting Vel-Lab Research. We are shutdown for the holidays. We use this time to give our employees a well deserved break, we also take the opportunity to thouroghly clean our facilities and calibrate all our equipment. Your project is our top priority and we will return to it as soon as possible. I will personally return to the lab Dec. 27th and will attend to any urgent issues then. The full lab will be back the first week of January. Thank you for your understanding. Happy Holidays from everyone at Vel-Lab Arnie Jimenez Owner Vel-Lab Research From contact <@t> excaliburpathology.com Thu Dec 20 17:50:21 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Dec 20 17:50:34 2007 Subject: [Histonet] No Santa Claus Message-ID: <400599.4872.qm@web50106.mail.re2.yahoo.com> O my Claire, what a wonderful story!!! Merry christmas to all!!! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From ohenry <@t> dfw.net Thu Dec 20 18:56:32 2007 From: ohenry <@t> dfw.net (Susan Owens) Date: Thu Dec 20 18:57:01 2007 Subject: [Histonet] Holidays Message-ID: <003201c8436c$54e28740$c6f763d8@your4f1261a8e5> PLEASE, click on the below address if you want to smile..Happy Holidays to All...... --Also, this is great in 'full screen' so push F11. http://www.jacquielawson.com/viewcard.asp?code=1358544974108&source=jl999 SUSAN OWENS-TX From mtarango <@t> nvcancer.org Thu Dec 20 19:33:48 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Dec 20 19:34:15 2007 Subject: [Histonet] CPT Codes (consultations and IHC). Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48C9E@NVCIEXCH02.NVCI.org> Does anyone know if you can charge 88325 (in-depth consult) and 88342 (ihc marker) on the same case? Is 88342 a component of 88325?? Thanks! Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From JWEEMS <@t> sjha.org Thu Dec 20 20:40:16 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Dec 20 20:40:25 2007 Subject: [Histonet] CPT Codes (consultations and IHC). In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE01E48C9E@NVCIEXCH02.NVCI.org> References: <5AEC610C1CE02945BD63A395BA763EDE01E48C9E@NVCIEXCH02.NVCI.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F46D2@sjhaexc02.sjha.org> Only if you do the stain... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Thursday, December 20, 2007 8:34 PM To: Histonet Subject: [Histonet] CPT Codes (consultations and IHC). Does anyone know if you can charge 88325 (in-depth consult) and 88342 (ihc marker) on the same case? Is 88342 a component of 88325?? Thanks! Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Dec 21 03:36:20 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Dec 21 03:36:37 2007 Subject: [Histonet] IHC for VIAS In-Reply-To: <20071220214912.4028gmx1@mx083.gmx.net> Message-ID: <001d01c843b4$f223b5e0$eeeea8c0@dielangs.at> If you have the possibility minimize the block-size just for the tumor. Score with a needle around the interesting areal and eliminate the fat. Be sure, that no water is under the section, dry the slides upright. We usually put the slides directly from cutting in the XT and have hardly problems. Gudrun Lang -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Thursday, December 20, 2007 4:28 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] IHC for VIAS We are having problem with tissue falling off the slides when we run the breast panel on the Ventana XT for examination with the VIAS instrument. The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and place them in 45 degree oven overnight and they still fall of. Any suggestions? Thanks. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Fri Dec 21 07:28:52 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Dec 21 07:29:05 2007 Subject: [Histonet] Falling tissue Message-ID: From: "Godfrey Guerzon" Subject: [Histonet] IHC for VIAS To: Cc: Message-ID: Content-Type: text/plain; charset=US-ASCII We are having problem with tissue falling off the slides when we run the breast panel on the Ventana XT for examination with the VIAS instrument. The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and place them in 45 degree oven overnight and they still fall of. Any suggestions? Thanks. HI Godfrey, Are these the ONLY slides that the tissue is falling off? That seems interesting! If you can run the XT and everything else stays on, I'd look at the differences in your protocols, between the breast markers and everything else...I understand your protocols can't be changed if you are using the FDA approved protocols, but you might be able to determine the problem and adjust. If you are drying overnight at 45'C do you think the oven is holding moisture in? Sometimes these ovens are more like incubators and they don't really dry out the paraffin. Just my 2 cent's worth but I would put the slides where there is good airflow, even if it's room temp, overnight(perhaps under a hood?) . Then I would put them in a 60'C oven for about 20 minutes. This ensures complete drying and then at the higher temp "bakes" the tissue onto the slide. Your XT should do a little "baking" too before it begins the Depar process. One other test I would do is run 2 sets of slides. One protocol using the depar function on the XT and the other set to run without the depar and depar these slides offline...just to see if you can determine what step the tissues starts washing off. Hope this helps. Becky Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From talulahgosh <@t> gmail.com Fri Dec 21 07:59:41 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Dec 21 07:59:55 2007 Subject: [Histonet] Off topic: 'tis the season for Nylarthotep!! Message-ID: Sorry if you're not a worshipper of Cthulu, but I thought this story was good. http://www.dagonbytes.com/thelibrary/lovecraft/thecallofcthulhu.htm (scroll down for the text of the story) Cthulhu fhtagn! Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From judi.ford <@t> roche.com Fri Dec 21 08:23:16 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Fri Dec 21 08:23:51 2007 Subject: [Histonet] subscribe digest Message-ID: Judi Ford, BA, HTL(ASCP) Research Associate III Roche Palo Alto 3431 Hillview Ave Palo Alto, CA 94304 650+855-6122 From jfrank <@t> med.unc.edu Fri Dec 21 09:07:28 2007 From: jfrank <@t> med.unc.edu (Jonathan Frank) Date: Fri Dec 21 09:07:17 2007 Subject: [Histonet] BrdU/DCX problem Message-ID: <011101c843e3$340418a0$1d6413ac@C26124> Dear list, I am trying to double label cells with BrdU and Doublecortin (DCX) in the adult rat brain and I am running into some issues. I have been able to get both antibodies to work well individually and together in the same tissue but I am not getting the co-labeling of cells that the literature supports. Our na?ve untreated rats do not show any co-labeling of DCX and BrdU while the literature shows that there should be quite a bit of this occurring. Since BrdU is nuclear and DCX is cytoplasmic I do not believe that one is blocking the other but maybe I am forgetting something. I was wondering if anyone has used BrdU/DCX and have had similar problems or if there is something that I am missing. Below are some details of the protocol. Animal: Adult female Sprague dawley rats (~275g) Tissue: perfused and fixed in 4% paraformaldehyde (overnight) and frozen in liquid nitrogen Slide prep: 10um sections Primary AB?s: Abcam polyclonal rabbit anti-DCX (ab 18723) Abcam polyclonal sheep anti-BrdU (ab 1893) Secondary detection: Alexa Fluor donkey anti-sheep Alexa Fluor goat anti- rabbit 1. Incubate slides for 30? in Citrate buffer pH 6.0 at 95oC in water bath. Place citrate buffer in plastic slide cover and then into water bath. Set water bath to 95oC. 2. Allow slides to cool in citrate buffer for 10? 3. Wash 2 x 5? in 1x PBS 4. Place 2-3 drops of 2N HCl on each slide and incubate for 20? at room temperature. 5. Wash 2 x 5? in 1x PBS 6. Wash with 0.1M Tris buffer 2 x 5 minutes 7. Wash 2 x 5? in 1x PBS 8. Block with 10% normal donkey serum in washing buffer for 60? 9. Incubate in primary BrdU antibody overnight at room temperature (dilution: 1.5uL/mL, or 5ug/mL). a. Make necessary amount of BrdU antibody (1.5:1000 dilution in washing buffer). 10. Gently tap off BrdU primary antibody. 11. Wash 5 x 5? in washing buffer. 12. Incubate for 60 minutes in secondary antibody for BrdU (dilution 2ul/1mL, or 1:500) 13. Wash 2 x 5? in washing buffer 14. Wash 2 x 5? in 1x PBS 15. Block for 1 hour in blocking buffer (8% goat serum, 0.3% Bovine serum albumin, 0.3% Triton X-100, in 1x PBS) at room temperature. For DCX 16. Incubate with DCX primary antibody (1:1000 dilution in washing buffer) overnight at room temperature a. Make necessary amount of DCX (1:1000 dilution in washing buffer) 17. Wash 5 times for 5 minutes each with washing buffer. Tap excess buffer off of slide. 18. Add secondary antibody (AF goat anti-rabbit 488 1:500 in washing buffer) and incubate for 60 minutes at room temperature. 19. Wash 2 x 5? in washing buffer 20. Wash once for 5 minutes in distilled water. 21. Coverslip 22. Store in cool dark place (refrigerator) Jon Frank Manager, Small Animal Resuscitation Research Lab Department of Emergency Medicine UNC-Chapel Hill jfrank@med.unc.edu 919-966-6442/919-966-7998 From kmerriam2003 <@t> yahoo.com Fri Dec 21 09:34:32 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Dec 21 09:34:43 2007 Subject: [Histonet] FISH literature Message-ID: <391683.54003.qm@web50305.mail.re2.yahoo.com> Hi, I have been asked to do some FISH (which I have never done before). Are there any good websites out there that can explain the concepts and techniques to a beginner? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From rjbuesa <@t> yahoo.com Fri Dec 21 09:54:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 21 09:54:45 2007 Subject: [Histonet] FISH literature In-Reply-To: <391683.54003.qm@web50305.mail.re2.yahoo.com> Message-ID: <127838.32966.qm@web61220.mail.yahoo.com> Try to contact an Abbott representative, they have very good probes and a lot of literature to go with. Ren? J. Kim Merriam wrote: Hi, I have been asked to do some FISH (which I have never done before). Are there any good websites out there that can explain the concepts and techniques to a beginner? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gu.lang <@t> gmx.at Fri Dec 21 10:23:11 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Dec 21 10:23:29 2007 Subject: AW: [Histonet] FISH literature In-Reply-To: <391683.54003.qm@web50305.mail.re2.yahoo.com> Message-ID: <000001c843ed$c7fdfa80$eeeea8c0@dielangs.at> http://www.slh.wisc.edu/wps/wcm/connect/extranet/cytogenetics/procedures/fis h/index.php http://www.dako.com/index/support/support_elearning.htm http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/InSi_toc.htm http://www.pathologen-luebeck.de/Methoden/Molekularpath/in-situ-Hybridisieru ng/in-situ-hybridisierung.html I find this quite informative. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kim Merriam Gesendet: Freitag, 21. Dezember 2007 16:35 An: Histonet Betreff: [Histonet] FISH literature Hi, I have been asked to do some FISH (which I have never done before). Are there any good websites out there that can explain the concepts and techniques to a beginner? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________ ________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Dec 21 10:48:46 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Dec 21 10:49:08 2007 Subject: [Histonet] FISH literature In-Reply-To: <391683.54003.qm@web50305.mail.re2.yahoo.com> Message-ID: <000001c843f1$5db39a50$d00f7ca5@lurie.northwestern.edu> Kim, Abbott Molecular (formerly Vysis) also has training classes for FISH- procedure and screening. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, December 21, 2007 9:35 AM To: Histonet Subject: [Histonet] FISH literature Hi, I have been asked to do some FISH (which I have never done before). Are there any good websites out there that can explain the concepts and techniques to a beginner? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________ ________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Dec 21 10:53:21 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Dec 21 10:53:33 2007 Subject: [Histonet] Happy Holidays!! Message-ID: Hi Histonetters, I just wanted to take a moment of your time and say Happy Holidays. I appreciate your acceptance of me as a member of the listserv. I hope everyone has a very Merry Christmas and a Happy New Year. RELIA Solutions offices will be closed from 12/25-0101. If you need to reach me I will be available by cell phone at 407-353-5070. See you next year - Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From oshel1pe <@t> cmich.edu Fri Dec 21 11:43:55 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Dec 21 11:44:52 2007 Subject: [Histonet] Off topic: 'tis the season for Nylarthotep!! In-Reply-To: References: Message-ID: Wow, it's Friday and no bites. But I'm more of a Yog-Sototh type anyway. Phil >Sorry if you're not a worshipper of Cthulu, but I thought this story was good. >http://www.dagonbytes.com/thelibrary/lovecraft/thecallofcthulhu.htm >(scroll down for the text of the story) > >Cthulhu fhtagn! >Emily >-- >"Prosperity ripened the principle of decay...and, as soon as time or >accident and removed the artificial supports, the stupendous fabric >yielded to the pressure of its own weight...instead of inquiring why >the Roman Empire was destroyed we should rather be surprised that it >has subsisted for so long" >-Edward Gibbon, Decline and Fall of the Roman Empire > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From info <@t> hphisto.com Fri Dec 21 12:12:41 2007 From: info <@t> hphisto.com (info@hphisto.com) Date: Fri Dec 21 12:12:52 2007 Subject: [Histonet] Need short term work Message-ID: <1402041.493841198260761359.JavaMail.servlet@perfora> Hello histonetters, and Season's Greeting I find myself between positions, and am hoping that there is a lab out there that could use a technician for 2-3 weeks? 30 years experience, References and CV upon request. Please e-mail me directly if I can be of service to you. Bill O'Donnell HT (ASCP) QIHC High Performance Histology Services LLC www.hphisto.com From arnie.jimenez <@t> vel-lab.com Fri Dec 21 12:10:32 2007 From: arnie.jimenez <@t> vel-lab.com (arnie.jimenez@vel-lab.com) Date: Fri Dec 21 12:21:53 2007 Subject: [Histonet] Happy Holidays! Message-ID: <20071221181032.20574.qmail@ux-vhost05.dllstx2.theplanet.com> This is an automated response. Thank you for contacting Vel-Lab Research. We are shutdown for the holidays. We use this time to give our employees a well deserved break, we also take the opportunity to thouroghly clean our facilities and calibrate all our equipment. Your project is our top priority and we will return to it as soon as possible. I will personally return to the lab Dec. 27th and will attend to any urgent issues then. The full lab will be back the first week of January. Thank you for your understanding. Happy Holidays from everyone at Vel-Lab Arnie Jimenez Owner Vel-Lab Research From sjchtascp <@t> yahoo.com Fri Dec 21 14:05:36 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Dec 21 14:05:48 2007 Subject: [Histonet] HT position wanted Message-ID: <786741.33673.qm@web38203.mail.mud.yahoo.com> I'm looking for an HT position in the South central portion of WI or North central part of Ill. I am also able to do short term temp employment after the 2nd week of Jan 2008. Thanks, Steve --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mulpiece <@t> yahoo.com Fri Dec 21 14:07:25 2007 From: mulpiece <@t> yahoo.com (Michael Muller) Date: Fri Dec 21 14:07:36 2007 Subject: [Histonet] ihc controls falling off Message-ID: <985732.29041.qm@web54111.mail.re2.yahoo.com> this is my first submission, so hereit goes.... I was having the same trouble with controls falling off for almost all those ab's also. I tried using a different control block and they stay on now. With all the variables in fixation and processing, that could be all you need. Michael Muller Converge Dx Peabody, MA ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From jseaton <@t> wlgore.com Fri Dec 21 17:02:38 2007 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Fri Dec 21 17:02:59 2007 Subject: [Histonet] Janella Seaton/WLGORE is out of the office. Message-ID: I will be out of the office starting 12/21/2007 and will not return until 12/26/2007. I will respond to your message when I return. From RSRICHMOND <@t> aol.com Fri Dec 21 17:39:11 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Dec 21 17:39:22 2007 Subject: [Histonet] Re: Off topic: 'tis the season for Nylarthotep!! Message-ID: Ph'nglui mglw'nafh Cthulhu R'lyeh wgah'nagl fhtagn! From pruegg <@t> ihctech.net Fri Dec 21 22:34:57 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Dec 21 22:35:20 2007 Subject: [Histonet] mitachrondrial disease Message-ID: <005201c84454$025118f0$0202a8c0@ihctechq9h2qof> Can someone recommend a histology approach to demonstrate diseased mitochondria, the investigators I am working with are cardiologist dealing with genetic defects. Thank you and happy holidays, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org From bakevictoria <@t> gmail.com Sat Dec 22 11:07:43 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Dec 22 11:08:11 2007 Subject: [Histonet] IHC for VIAS In-Reply-To: References: Message-ID: <4f016b690712220907r1a1da82dw9f37c7788afd5894@mail.gmail.com> Guerzon, I had the same thing happen to me when trying to do these panels on breast tissue. The biggest problem is the fatty/CT tissue that is associated with the tissue itself. After trying some of the same suggestions others have given you and still not having the best results we opt'd to first reviewing the H&E, identifying the areas of interest and coring the block using a dermal punch (they come in different sizes). We then air dried the section over night - roughly 18 hours. An hour in a 56 degree oven was done off of the automated stainer prior to starting the staining process. I realize that in the clinical setting coring a block isn't possible, but scoring the area(s) on a slide is possible. If you are interested I can give the protocol I used to do this. It is somewhat labor intensive, but it is somewhere for you to start from. Breast tissue is very fatty and unless it is grossed in and processed properly, the residual water or fluids remaining in the tissue are what is causing your sections to come off during the staining process. By reducing the surface area to the site in question you stand a better chance of keeping the area in question on the slide and also improve the quality of the staining. Happy Holidays Vikki Baker On 12/20/07, Godfrey Guerzon wrote: > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rchiovetti <@t> yahoo.com Sat Dec 22 12:26:27 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Sat Dec 22 12:26:39 2007 Subject: [Histonet] mitachrondrial disease Message-ID: <314619.6939.qm@web58915.mail.re1.yahoo.com> Patsy, Is EM a possibility? The mitochondria in patients w/ some of the inherited mito diseases can be really strange-looking. If that's not possible, maybe some special staining can help. Gomori trichrome or even H&E can show "ragged red fibers" (probably due to the overabundance of mitochondria). Sudan can stain excess lipid (if it's there). Cytochrome oxidase (COX) can be reduced or absent in some mito diseases, and It looks like there can also be differences in succinic dehydrogenase (SDH) staining. Take a look at: ...just my 2 cents' worth... Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments www.swpinet.com Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: "pruegg@ihctech.net" To: Histonet Sent: Friday, December 21, 2007 9:34:57 PM Subject: [Histonet] mitachrondrial disease Can someone recommend a histology approach to demonstrate diseased mitochondria, the investigators I am working with are cardiologist dealing with genetic defects. Thank you and happy holidays, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From SHargrove <@t> urhcs.org Sat Dec 22 16:49:11 2007 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Sat Dec 22 16:49:40 2007 Subject: [Histonet] Susie Hargrove is out of the office. Message-ID: I will be out of the office starting 12/22/2007 and will not return until 01/02/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. From hcooper7 <@t> triad.rr.com Sat Dec 22 17:42:15 2007 From: hcooper7 <@t> triad.rr.com (Heather Cooper) Date: Sat Dec 22 17:44:22 2007 Subject: [Histonet] QIHC Message-ID: <000a01c844f4$4869ee70$27fa4c47@jcooper> I am planning to take the QIHC exam in February and am looking for advice/info on content. The suggested reading list is incredibly broad and I want to make sure I am focusing on the right areas. Thanks! From Jerry <@t> ralambusa.com Sun Dec 23 06:55:33 2007 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Sun Dec 23 06:55:50 2007 Subject: [Histonet] NY State (and other state and regional meetings) Message-ID: <3855F92002259948A66A8CA2D16E3A4F05A6A7@server.ralambusa.com> I thank all who have sent me information on State and regional meetings, but I still have some gaps and I would like to firm up my schedule for the next year, I would really appreciate information for any state or regional meeting for 2008. Please feel free to email me directly or post on histonet. Thanks and have a great holiday season! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ From Robert.Fauck <@t> ccdhb.org.nz Sun Dec 23 19:56:33 2007 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB]) Date: Sun Dec 23 19:57:04 2007 Subject: [Histonet] FW: SelecTech of Surgipath Message-ID: <5587F098B7C4EE489C8BE204E9565548248CF5@wn0nteml05.hiq.net.nz> ________________________________ From: Robert Fauck [CCDHB] Sent: Thursday, 20 December 2007 3:13 p.m. To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: SelecTech of Surgipath H i Everyone, I like to know if somebody can advise us what is the optimal staining time in the Haematoxilyn of SelecTech of Surgipath, and what exactly is the function of the Define solution? Thanks in advance for your help. Robert Fauck This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) No Viruses were detected in this message. HealthIntelligence eMail Filter Service From asmith <@t> mail.barry.edu Mon Dec 24 08:50:18 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Dec 24 08:51:23 2007 Subject: [Histonet] adipsin Message-ID: Does anyone in histoland have a protocol for IHC detection of adipsin or complement D in ffpe sections? Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 From rjbuesa <@t> yahoo.com Mon Dec 24 09:28:49 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 24 09:29:01 2007 Subject: [Histonet] FW: SelecTech of Surgipath In-Reply-To: <5587F098B7C4EE489C8BE204E9565548248CF5@wn0nteml05.hiq.net.nz> Message-ID: <317280.33248.qm@web61218.mail.yahoo.com> "Define" solution is a fancy name for differentiating (acid) solution after hematox. Ren? J. "Robert Fauck [CCDHB]" wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From macantu <@t> mdanderson.org Mon Dec 24 12:01:28 2007 From: macantu <@t> mdanderson.org (macantu@mdanderson.org) Date: Mon Dec 24 12:02:06 2007 Subject: [Histonet] Mayra A. Cantu/HOU/UTMDACC is out of the office. Message-ID: I will be out of the office starting 12/24/2007 and will not return until 12/28/2007. I will respond to your message when I return. From lpwenk <@t> sbcglobal.net Mon Dec 24 17:07:10 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Dec 24 17:07:31 2007 Subject: [Histonet] Golgi Cox Message-ID: <000701c84681$b6566560$0202a8c0@HPPav2> Left over from a question from another histonetter from a couple of weeks ago, where I added other questions to the end. I never got a reply, and I'm really curious about the Golgi-Cox fixation technique - why it's used, how the fixative is disposed of afterwards, why only researchers use it and routine histology labs don't seem to, is there are IHC equivalent, etc. So read below for my original comments and questions. I'd really love to hear about this from researchers and non-researchers. (And figuring many people are off over the holidays, it might be a slow enough time to get a dialogue going on this topic.) I really don't know anything about this technique, and would love to learn the WHY's of this procedure(the HOW's I can read in books). Thanks. "I have a couple of questions, for anyone in the Histonet community. This Golgi-Cox fixation procedure comes up a on occasion on Histonet, mostly from researchers. How do these labs dispose of the chemicals afterwards? Mercuric chloride cannot be dumped down any sink, and most (but not all) water/sewer treatment plants won't allow potassium dichromate to be disposed down the sink either. And how do researchers dispose of the water and alcohol and xylene in the processing afterwards, where mercuric chloride and potassium dichromate are being pulled out of the tissue into the processing solutions? Can't these tissues be fixed in formalin (or some other less toxic fixative than mercury and chromium), and then IHC procedures done, such as antibodies against GFAP or neurofilaments or NSE? I work in a hospital, and am just wondering why this Golgi-Cox procedure is needed by researchers, but doesn't seem to be needed by clinical histology laboratories." Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From bob.nienhuis <@t> gmail.com Wed Dec 26 11:53:25 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Wed Dec 26 15:16:31 2007 Subject: [Histonet] Golgi Cox In-Reply-To: <000701c84681$b6566560$0202a8c0@HPPav2> References: <000701c84681$b6566560$0202a8c0@HPPav2> Message-ID: <45109da50712260953w32c57d3dj383633bba7311102@mail.gmail.com> Re: Golgi-Cox Why is it only used by researchers? Well, as a University/ VA researcher who occasionally uses Golgi-Cox, I'm not sure why it is not used clinically. I do have a few guesses though. 1. Time. It takes several weeks to do the the impregnation, and I would guess that clinicians want answers STAT. 2. I don't know of any conditions for which it is particularly useful diagnostically. In general, Golgi-Cox stains only about 1-2 % of neural (brain) cells, but those that it does stain, it stains completely, with the full dendritic arborization and displays dendritic spines nicely. I think it would take a combination of several IHC stains to get the same information. Also, IHC would stain ALL of the cells, not just a few, thereby making it hard to see which dendrite belongs to which cell body. We use Golgi as a cell morphology and anatomy tool. I'm not sure of much in the way of clinical relevance. Re: Disposal We send all of the waste to a disposal company. We are OK with that as the costs for this don't come out of OUR budget or grant funds. Bob Nienhuis UCLA / Sepulveda VA Medical Center Los Angeles On Dec 24, 2007 3:07 PM, Lee & Peggy Wenk wrote: > Left over from a question from another histonetter from a couple of weeks > ago, where I added other questions to the end. I never got a reply, and > I'm > really curious about the Golgi-Cox fixation technique - why it's used, how > the fixative is disposed of afterwards, why only researchers use it and > routine histology labs don't seem to, is there are IHC equivalent, etc. So > read below for my original comments and questions. I'd really love to hear > about this from researchers and non-researchers. (And figuring many people > are off over the holidays, it might be a slow enough time to get a > dialogue > going on this topic.) I really don't know anything about this technique, > and > would love to learn the WHY's of this procedure(the HOW's I can read in > books). Thanks. > > "I have a couple of questions, for anyone in the Histonet community. This > Golgi-Cox fixation procedure comes up a on occasion on Histonet, mostly > from > researchers. How do these labs dispose of the chemicals afterwards? > Mercuric > chloride cannot be dumped down any sink, and most (but not all) > water/sewer > treatment plants won't allow potassium dichromate to be disposed down the > sink either. And how do researchers dispose of the water and alcohol and > xylene in the processing afterwards, where mercuric chloride and potassium > dichromate are being pulled out of the tissue into the processing > solutions? > > Can't these tissues be fixed in formalin (or some other less toxic > fixative > than mercury and chromium), and then IHC procedures done, such as > antibodies > against GFAP or neurofilaments or NSE? I work in a hospital, and am just > wondering why this Golgi-Cox procedure is needed by researchers, but > doesn't > seem to be needed by clinical histology laboratories." > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Bauer.Karen <@t> mayo.edu Wed Dec 26 12:39:12 2007 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Wed Dec 26 15:16:33 2007 Subject: [Histonet] Destaining Cytology Slides Message-ID: Hello to all... First, I would like to thank everyone for their help with our tissue adherence problem. I believe we were touching the slides too much before putting the tissue on them and we have really been watching that. Especially now, with winter and the cold weather, since we tend to use a lot of lotion on our hands. Sections have been staying on better, so hopefully that was the problem. Secondly, a question about destaining cytology smears before staining them with an IP stain. Do you or don't you destain the smear before running the IP stain. I've searched many articles and it seems to be 50/50. Some do and some don't. We regularly do not destain the smears, since they seem to destain with the reagents during the run and turn out fine. One of our pathologists started asking for the smears to be destained first and they look fine as well. So, if both look good, why destain beforehand? Does the PAP or Diff Quik stain really mask the antigens enough to prevent IP staining? Some articles stated that decolorizing could actually denature the antigen and prevent antibody binding. Any explanations would be helpful. Thanks in advance, Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Keith.Danielson <@t> uphs.upenn.edu Wed Dec 26 15:37:21 2007 From: Keith.Danielson <@t> uphs.upenn.edu (Danielson, Keith) Date: Wed Dec 26 15:37:49 2007 Subject: [Histonet] FW: New position - Pennsylvania Hospital Message-ID: <84D11048BB18234D8A56474645E8EC020829EDC1@uphsmbx5.UPHS.PENNHEALTH.PRV> We have an immediate opening for a histotechnologist to work full time in the immunohistochemistry lab at the Pennsylvania Hospital in Philadelphia, PA. Excellent tissue sectioning skills are required and knowledge of automated immunostaining systems (e.g. the Ventana Benchmark XT) is a plus. Interested applicants should send a cover letter and resume to: Keith Danielson, PhD Supervisor, Immunohistochem. & Mol. Biol. Lab Department of Pathology Pennsylvania Hospital Rm. 862, Preston Building 800 Spruce Street Philadelphia, PA 19107 E-mail: keith.danielson@uphs.upenn.edu Phone: 215 829-7726 Fax: 215 829-5017 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rjbuesa <@t> yahoo.com Wed Dec 26 15:49:44 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 26 15:50:07 2007 Subject: [Histonet] Destaining Cytology Slides In-Reply-To: Message-ID: <73639.80768.qm@web61216.mail.yahoo.com> Karen: I belong to the 50% who destain. Why risk the IHC reaction with any type of masking if destaining is readily done? Ren? J. "Bauer, Karen" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From hodges420 <@t> msn.com Thu Dec 27 09:55:45 2007 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Thu Dec 27 09:56:11 2007 Subject: [Histonet] gloves and cutting Message-ID: Good morning all, A question came up in our lab meeting today. Are gloves needed as PPE for cutting or is that an area we could just use our lab coats as proper PPE, and is that exceptable for JC and CAP. Thanks for your in put Tere Hodges, Tech Specialist St Mart's Hospital Tucson, Az. 85724 _________________________________________________________________ i?m is proud to present Cause Effect, a series about real people making a difference. http://im.live.com/Messenger/IM/MTV/?source=text_Cause_Effect From mpence <@t> grhs.net Thu Dec 27 10:04:47 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Dec 27 10:05:12 2007 Subject: [Histonet] gloves and cutting In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C831@IS-E2K3.grhs.net> Are you talking "cutting" as grossing in cutting or sectioning with a microtome cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARY T HODGES Sent: Thursday, December 27, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gloves and cutting Good morning all, A question came up in our lab meeting today. Are gloves needed as PPE for cutting or is that an area we could just use our lab coats as proper PPE, and is that exceptable for JC and CAP. Thanks for your in put Tere Hodges, Tech Specialist St Mart's Hospital Tucson, Az. 85724 _________________________________________________________________ i'm is proud to present Cause Effect, a series about real people making a difference. http://im.live.com/Messenger/IM/MTV/?source=text_Cause_Effect___________ ____________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Thu Dec 27 13:03:41 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Dec 27 13:04:02 2007 Subject: [Histonet] hooves Message-ID: Could someone in veterinary histology share their SOP for softening hooves? I have had some deer hooves in Nair for much longer than I ever had to before and I can't even make a nick in them. I did have to buy a new bottle and am beginning to think they changed their formula. Thank you, Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University From debbiekeith <@t> cox.net Thu Dec 27 14:03:37 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Dec 27 14:04:02 2007 Subject: [Histonet] Mohs and the new Multiple procedure reduction rule.... Message-ID: <20071227200339.CZRG129.eastrmmtao101.cox.net@eastrmimpo02.cox.net> hi all! I was wondering how most surgeons are responding to the new medicare rules. Is your facility still doing multiple tumors? Are surgeons repairing on the same day or the following day (to avoid reduction of payment)? respectfully, debbie keith, HT(ASCP) -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.17.11/1200 - Release Date: 12/27/2007 1:34 PM From rjbuesa <@t> yahoo.com Thu Dec 27 15:10:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 27 15:11:09 2007 Subject: [Histonet] hooves In-Reply-To: Message-ID: <481892.7493.qm@web61211.mail.yahoo.com> Place them (after fixation) in 10% sodium hydroxyde. Touch them every hour until they soften. Wash them with plenty of running water afterwards until there is no more "soapy" feeling in your fingers after you touch them, and process as usual. Ren? J. Roberta Horner wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From tracy.bergeron <@t> biogenidec.com Fri Dec 28 07:06:49 2007 From: tracy.bergeron <@t> biogenidec.com (Tracy Bergeron) Date: Fri Dec 28 07:07:15 2007 Subject: [Histonet] Alcohols - clarification - help needed In-Reply-To: Message-ID: Hi folks, Our lab is trying to trying to change to an alcohol that is less harsh on rodent tissue than your typical reagent grade alcohols can be. We've considered moving to pure EtOH, but the company we work for is not all that keen on dealing with the regulations etc involved with it's use. So.... We've been looking at the products offered by Pharmco-Aaper and have come across some confusing information, at least to me anyway. Yes I have emailed the sales rep and am waiting for her reply. Pure EtOH is listed as 200 proof and anhydrous, 95% EtOH is listed as 190 Proof. When you look at the denatured alcohols you end up seeing EtOH mixed with various and sundry solvents like Toluene, unleaded gasoline, etc etc. But where I get stuck is when the spec sheet says that the EtOH is 190 proof in toluene, but does not list the water content, yet does not say it is anhydrous. Can we assume that a 190 proof EtOH is these cases is the same as 95% EtOH with 5% water? My assumption is that if it doesn't say it is anhydrous then it is not whether the water content is listed on the spec sheet or not. Have any of you out there used the Aaper denatured alcohols, that are not the reagent grade? Also regarding the pure EtOH that is ACS and is a synthetic product, is that regulated by the ATF, as is grain alcohol? My limited understanding of synthetic vs grain alcohol is that the synthetic is not used in food products - and I don't think it is drinkable, but I could be wrong. Any ides on this. Thanks. Sincerely, Tracy E. Bergeron, B.S., HT, HTL (ASCP) Associate Scientist III, Pathology Comparative Pathology Laboratory Biogen Idec 14 Cambridge Center Cambridge, MA 02142 Direct: 617-914-1115 Fax: 617-679-3208 From rjbuesa <@t> yahoo.com Fri Dec 28 07:25:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 28 07:26:13 2007 Subject: [Histonet] Alcohols - clarification - help needed In-Reply-To: Message-ID: <545073.9046.qm@web61213.mail.yahoo.com> Tracy: Let me try to answer your question: 1- as you have already figured out the "proof" value = (ethanol contents) X 2 so a "200 proof" y 100% or pure ethanol. 2- ethanol is usually "denatured" to make in undrinkable by its "fans" therefore all the regulations involved in selling it "pure" 3- a 190 proof = 95% ethanol + 5% of "something" but usually not water, and it could be toluene or any other thing (sometimes methanol). Probably you have been using methilated alcohol (like in the UK) and if you cannot use pure ethanol for administrative reasons, I would advise you to switch to isopropanol = 2-propanol = propanol that is less harsh than both methanol and ethanol for your particular type of tissue and that you can obtain pure. Ren? J. Tracy Bergeron wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From karenadams <@t> comcast.net Fri Dec 28 08:38:14 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Fri Dec 28 08:38:37 2007 Subject: [Histonet] Fla Histonetters Message-ID: <122820071438.25242.47750A560004CE270000629A22070229339C030E0B0E020A9D0E05@comcast.net> I will be traveling to the Jacksonville - Gainesville area next week and am wondering if I can visit any histo labs in those surrounding areas. We will begin remodeling the end of January here and I simply want to look around for any innovative ideas. Please e mail me back if this is feasible. Thank you. -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From lblazek <@t> digestivespecialists.com Fri Dec 28 08:50:58 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Dec 28 08:50:28 2007 Subject: [Histonet] new stainer Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4E0E@bruexchange1.digestivespecialists.com> Good morning everyone, I am getting ready to purchase a new H & E stainer. What stainer is the best to stain about 300 - 400 slides a day? Thanks for your help. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From Sharon.Davis-Devine <@t> carle.com Fri Dec 28 09:37:51 2007 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Fri Dec 28 09:38:15 2007 Subject: [Histonet] IHC stainers Message-ID: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> I have recently been given the assignment of implementing a new and updated IHC system in our Histology department. We currently use a Dako Autostainer which we have used for several years and needs to be updated. I have limited in expertise in this are so am asking for all you Histonetters who have experience with this to give me some advice on which stainer to purchase. Let me know all of your thoughts both good and bad please. Thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From rjbuesa <@t> yahoo.com Fri Dec 28 09:56:07 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 28 09:56:30 2007 Subject: [Histonet] IHC stainers In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> Message-ID: <217012.73149.qm@web61219.mail.yahoo.com> Everybody seems to be leaning to recommending the BondMax from Leica microsystems (originally BioSystems) as the best yet. Ren? J. "Sharon.Davis-Devine" wrote: I --------------------------------- Never miss a thing. Make Yahoo your homepage. From jmahoney <@t> alegent.org Fri Dec 28 10:17:45 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Dec 28 10:18:16 2007 Subject: [Histonet] IHC stainers In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> Message-ID: <4774CD490200003C00025596@gwia.alegent.org> Knowing this could start a histonet war I am reluctant to participate but we use Ventana and love it. We have several Benchmarks and one XT. The main reason we like Ventana is that is truly a walk away system. It is pretty much fool proof. With the shortage of HT's we have found that lab aids can do many of the tasks associated with IHC when using the Ventana instruments. Volume is not a problem because the runs are relatively short and we usually do three runs on each instrument every day, one being an overnight run. Some of my techs and I have experience with other instruments and we all agree that Ventana is the easiest and we get the most consistent quality. I know cost is an issue for some labs, but the time it allows my Histo techs to do things that only HT's can do makes the cost worth it. Jan Mahoney Omaha, NE From Susan.Weber2 <@t> va.gov Fri Dec 28 10:35:08 2007 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri Dec 28 10:35:34 2007 Subject: [Histonet] IHC stainers In-Reply-To: <4774CD490200003C00025596@gwia.alegent.org> References: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> <4774CD490200003C00025596@gwia.alegent.org> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB902276CA4@VHAV10MSGA1.v10.med.va.gov> OK, So now I shall supply fuel to the fire (so to speak) and also give my vote to Ventana! Being very new to doing IHC testing myself, I can truly say that the Benchmark XT is very user friendly you basically set it and forget it. The training that Ventana provided both here and in Tucson, really gave me a great start. Yes, I know that you have a lot of proprietary reagents, but that does give very consistent staining quality. Yes, you can do offline dilutions if needed to set up your protocols and yes, you must use their Prep kits for Non-Ventana antibodies (a lot of Cell Marque antibodies are already ready for the Ventana system and do not require Prep kits) however, being able to free personell for other duties is worth it. Quality integrity does not vary from tech to tech, because it is all automated! I do admit to being ignorant of some of the other systems, but what little I know does support the fact of its ease of use. Just my 2 cents! Happy New Year to ALL!! Susan M. Weber, HT(ASCP) Louis Stokes Cleveland VA Medical Center Supervisor, Histology Laboratory (216) 791-3800 ext 6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, December 28, 2007 11:18 AM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC stainers Knowing this could start a histonet war I am reluctant to participate but we use Ventana and love it. We have several Benchmarks and one XT. The main reason we like Ventana is that is truly a walk away system. It is pretty much fool proof. With the shortage of HT's we have found that lab aids can do many of the tasks associated with IHC when using the Ventana instruments. Volume is not a problem because the runs are relatively short and we usually do three runs on each instrument every day, one being an overnight run. Some of my techs and I have experience with other instruments and we all agree that Ventana is the easiest and we get the most consistent quality. I know cost is an issue for some labs, but the time it allows my Histo techs to do things that only HT's can do makes the cost worth it. Jan Mahoney Omaha, NE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jgutierrez <@t> precisionpath.us Fri Dec 28 12:19:14 2007 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Fri Dec 28 12:25:11 2007 Subject: [Histonet] IHC stainers In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB902276CA4@VHAV10MSGA1.v10.med.va.gov> References: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com><4774CD490200003C00025596@gwia.alegent.org> <16C83872A53F4346AA9C3A18E3A3AAB902276CA4@VHAV10MSGA1.v10.med.va.gov> Message-ID: Amen, sister. My vote goes to Ventana for all the same reasons. They also have the best tech support and are very fast to come in and fix any problems that may arise. Juan C. Gutierrez, HT(ASCP) Precision Pathology Services (210)646-0890 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weber, Susan (VHACLE) Sent: Friday, December 28, 2007 10:35 AM To: Janice Mahoney; Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC stainers OK, So now I shall supply fuel to the fire (so to speak) and also give my vote to Ventana! Being very new to doing IHC testing myself, I can truly say that the Benchmark XT is very user friendly you basically set it and forget it. The training that Ventana provided both here and in Tucson, really gave me a great start. Yes, I know that you have a lot of proprietary reagents, but that does give very consistent staining quality. Yes, you can do offline dilutions if needed to set up your protocols and yes, you must use their Prep kits for Non-Ventana antibodies (a lot of Cell Marque antibodies are already ready for the Ventana system and do not require Prep kits) however, being able to free personell for other duties is worth it. Quality integrity does not vary from tech to tech, because it is all automated! I do admit to being ignorant of some of the other systems, but what little I know does support the fact of its ease of use. Just my 2 cents! Happy New Year to ALL!! Susan M. Weber, HT(ASCP) Louis Stokes Cleveland VA Medical Center Supervisor, Histology Laboratory (216) 791-3800 ext 6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, December 28, 2007 11:18 AM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC stainers Knowing this could start a histonet war I am reluctant to participate but we use Ventana and love it. We have several Benchmarks and one XT. The main reason we like Ventana is that is truly a walk away system. It is pretty much fool proof. With the shortage of HT's we have found that lab aids can do many of the tasks associated with IHC when using the Ventana instruments. Volume is not a problem because the runs are relatively short and we usually do three runs on each instrument every day, one being an overnight run. Some of my techs and I have experience with other instruments and we all agree that Ventana is the easiest and we get the most consistent quality. I know cost is an issue for some labs, but the time it allows my Histo techs to do things that only HT's can do makes the cost worth it. Jan Mahoney Omaha, NE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Dec 28 16:22:20 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Dec 28 16:22:43 2007 Subject: [Histonet] RE: IHC stainers Message-ID: <718406.41069.qm@web50601.mail.re2.yahoo.com> Hi Sharon, Since I'm a former Ventana employee, I like the BenchMark...BUT...it's a preference. Let me ask you this: How experienced are your techs? How much off line time do you want to spend baking slides, deparaffinizing, pretreatments (antigent retreival), etc? All systems are good in their own right. I suggest you demo a couple of instruments, let your techs try them out, and go from there....hope this helps...good luck... Kim Tournear, HT (ASCP), QIHC ( ASCP) Tucson Medical Center (TMC) Histology Supervisor Tucson, AZ --------------------------------- Never miss a thing. Make Yahoo your homepage. From Tracey.Lenek <@t> CLS.ab.ca Fri Dec 28 16:27:04 2007 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Fri Dec 28 16:27:58 2007 Subject: [Histonet] Antibody Recommendations Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE06B7C689@mail1.calgary.com> Hi, Can anyone recommend suppliers for TPO, Galectin 3 and HMBE1? Thanks in advance, Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From slappycraw <@t> yahoo.com Fri Dec 28 18:34:00 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Dec 28 18:34:25 2007 Subject: [Histonet] IHC stainers In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> Message-ID: <463279.80979.qm@web53601.mail.re2.yahoo.com> Sharon: I have used both the Ventana and the Lab Vision, (the one Dako distributes because the only one Dako makes is called Eridan and it doesn't work) ,and several others not worth mentioning, but if I had my choice I would probably have several of each. If there are people with limited experience and knowledge in the area of IHC, then the Ventana is along those lines however if you are wanting to work up a novel antibody from scratch then you need an open platform where you can run an experiment and the Lab Vision gives you that freedom. "Sharon.Davis-Devine" wrote: I have recently been given the assignment of implementing a new and updated IHC system in our Histology department. We currently use a Dako Autostainer which we have used for several years and needs to be updated. I have limited in expertise in this are so am asking for all you Histonetters who have experience with this to give me some advice on which stainer to purchase. Let me know all of your thoughts both good and bad please. Thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From yourbiomed <@t> cox.net Fri Dec 28 23:24:20 2007 From: yourbiomed <@t> cox.net (YourBiomed.Com) Date: Fri Dec 28 23:24:38 2007 Subject: [Histonet] IHC stainers In-Reply-To: <463279.80979.qm@web53601.mail.re2.yahoo.com> References: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> <463279.80979.qm@web53601.mail.re2.yahoo.com> Message-ID: <000001c849db$10be94d0$323bbe70$@net> I'm a Biomedical Engineer and have lots of experience with all automated instruments, I know the Dako Systems, I too was a Ventana employee for over 3 1/2 years with them 7+ years experience with all their platforms, I also familiar with Lab Vision, Biocare Nemesis and plenty of others. I am certified and very capable of repairing and performing preventive maintenance on all and many other instruments. Please give me more info on your lab size, experience and what you are staining human, vet, and/or special stains? I think there are a lot of people that can help you out. I'll do what I can. Brad Yourbiomed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Friday, December 28, 2007 4:34 PM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC stainers Sharon: I have used both the Ventana and the Lab Vision, (the one Dako distributes because the only one Dako makes is called Eridan and it doesn't work) ,and several others not worth mentioning, but if I had my choice I would probably have several of each. If there are people with limited experience and knowledge in the area of IHC, then the Ventana is along those lines however if you are wanting to work up a novel antibody from scratch then you need an open platform where you can run an experiment and the Lab Vision gives you that freedom. "Sharon.Davis-Devine" wrote: I have recently been given the assignment of implementing a new and updated IHC system in our Histology department. We currently use a Dako Autostainer which we have used for several years and needs to be updated. I have limited in expertise in this are so am asking for all you Histonetters who have experience with this to give me some advice on which stainer to purchase. Let me know all of your thoughts both good and bad please. Thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ombadda3 <@t> gmail.com Sat Dec 29 05:46:55 2007 From: ombadda3 <@t> gmail.com (K M) Date: Sat Dec 29 05:47:18 2007 Subject: [Histonet] web sites explaining IHC technique step by step Message-ID: Hi histonetters Happy new year Iam asking for web sites which explain IHC techniques step by step using animations if possible. Thanks much From ROrr <@t> enh.org Sat Dec 29 09:22:38 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Sat Dec 29 09:32:14 2007 Subject: [Histonet] Bone holders? Message-ID: Help anyone? Hi Becky Linda would like me to find something else that would be safer than those uterine forceps that we use to have. I looked in the hardware area at Sears.com and found a couple of vise clamps and ratcheting clamps. I don't know if they will work or not. I was wondering if you might be able to ask on histonet what they cut their bones with if using a band saw, meaning what do they hold on to the bone with besides their hands. Thanks, see you next week. Beth Beth E. Richter, PA(ASCP) Evanston Northwestern Healthcare Evanston Hospital Surgical Pathology Rm. 1919A 847-570-4039 Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From JWEEMS <@t> sjha.org Sat Dec 29 10:24:23 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Dec 29 10:25:02 2007 Subject: [Histonet] Bone holders? References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B2C@sjhaexc02.sjha.org> We have a big vice mounted on a board - works for everything and is easily bleachable... j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Orr, Rebecca Sent: Sat 12/29/2007 10:22 AM To: histonet@lists.utsouthwestern.edu Cc: Richter, Beth Subject: [Histonet] Bone holders? Help anyone? Hi Becky Linda would like me to find something else that would be safer than those uterine forceps that we use to have. I looked in the hardware area at Sears.com and found a couple of vise clamps and ratcheting clamps. I don't know if they will work or not. I was wondering if you might be able to ask on histonet what they cut their bones with if using a band saw, meaning what do they hold on to the bone with besides their hands. Thanks, see you next week. Beth Beth E. Richter, PA(ASCP) Evanston Northwestern Healthcare Evanston Hospital Surgical Pathology Rm. 1919A 847-570-4039 Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Sat Dec 29 15:03:06 2007 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Dec 29 15:03:07 2007 Subject: [Histonet] RE: bone holders Message-ID: <001001c84a5e$35d59470$6501a8c0@DHXTS541> With a band saw, using pieces of 2 x 4 soft pine wood should hold the bones. You don't want the saw blade to hit a metal holder as the blade may shatter and dulled, but worst of all is the potential for injury to operator. We used this method to saw through whole lengths of femurs of calves\(huge!) and other animals when using a meat cutters band saw. It worked for human femoral heads too. Simple grasp the bone between the pieces of wood - a nice safe way to prevent losing a finger. You can soak the wood in bleach later, or simply discard for incineration. The wood is cheap and doesn't hurt the blade. Try to have more teeth per inch in a saw blade, you will get smoother, cleaner cuts, We were able to saw with water running on the blade, but that is not always possible. However it keeps the bone from being burned by high speed saws. Holding the bone this way allowed us to get precise 2 mm to 1 cm slabs for doing large decalcified bone samples. Good luck Gayle M. Callis HT,HTL,MT(ASCP) Bozeman MT From gayle.callis <@t> bresnan.net Sat Dec 29 15:09:52 2007 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Dec 29 15:09:52 2007 Subject: [Histonet] Re: Websites to demonstrate IHC methods Message-ID: <001701c84a5f$27548c20$6501a8c0@DHXTS541> Dear K M, You can find IHC methods from just about any company selling antibodies and their ancillary reagents. As for animated IHC, you can find cartoons for immunostaining on the Vector and DAKO websites, but not animated. However they are step by step with explanation. Go to www.IHCworld and look at all the methods found there. If you are learning, this site is superb for information. You can also access the DAKO handbook for immunostaining, and request a copy of the last edition although I think it is available in pdf form on the DAKO website. They will send you a hard copy. Happy New Year Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT From pruegg <@t> ihctech.net Sat Dec 29 16:07:35 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Dec 29 16:08:07 2007 Subject: [Histonet] IHC stainers In-Reply-To: <463279.80979.qm@web53601.mail.re2.yahoo.com> References: <44780C571F28624DBB446DE55C4D733A021E080F@EXCHANGEBE1.carle.com> <463279.80979.qm@web53601.mail.re2.yahoo.com> Message-ID: <005101c84a67$386725b0$0202a8c0@ihctechq9h2qof> Sharon, If you are happy with the Dako, let them offer suggestions to up date your system, you will certainly have the most flexibility using a dako system as it is completely open and much cheaper to use in my experience than being locked into detection systems for closed systems. For more closed systems, I have also heard really good things about Vision BioSystems (now LeicaBioSystems) machines. Ask Dako and Leica what they can do for you, make them vie for your business, you will get the best deal if they think they have to compete. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Friday, December 28, 2007 5:34 PM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC stainers Sharon: I have used both the Ventana and the Lab Vision, (the one Dako distributes because the only one Dako makes is called Eridan and it doesn't work) ,and several others not worth mentioning, but if I had my choice I would probably have several of each. If there are people with limited experience and knowledge in the area of IHC, then the Ventana is along those lines however if you are wanting to work up a novel antibody from scratch then you need an open platform where you can run an experiment and the Lab Vision gives you that freedom. "Sharon.Davis-Devine" wrote: I have recently been given the assignment of implementing a new and updated IHC system in our Histology department. We currently use a Dako Autostainer which we have used for several years and needs to be updated. I have limited in expertise in this are so am asking for all you Histonetters who have experience with this to give me some advice on which stainer to purchase. Let me know all of your thoughts both good and bad please. Thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sat Dec 29 17:16:49 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Dec 29 17:17:12 2007 Subject: [Histonet] IHC Stainers Message-ID: <582736990712291516o423bb64dge16d410c7933e786@mail.gmail.com> Hi, To delve into the mucky waters of this question is a slippery slope indeed. It honestly depends upon how much control you want to have (or to give up!) over the staining process and the chemicals you use. I must say that the Ventana is a FANTASTICALLY engineered bit of equipment. I would use a Ventana in a second if it had the versatility I want in an autostainer. The ability to heat the slides individually and to stir the reagents is spectacular! Sadly unless you want to cough up a fortune for the high end machine, you are left having to use the secondary antibodies and ancillaries they provide. I believe, (and I'm sure I'll be corrected if I'm wrong) the BondX is the same in this respect. Hearing this, one may think that I would reccommend the DAKO and if you read the archives you'd be right. Lately I have been less sure of that! They have been working on the Eridan for a long time and I'm beginning to think it is a lost cause! (Think Vista from Microsoft) The development is long postponed and some say the company is going in the direction of the above equipment and attempting to lock users into a secondary detection system. The Original DAKO is still great and I still reccommend it, but I also reccommend the Thermo(FisherShandonRichard-AllenLabVision...) It is as, if not more versatile than the DAKO since it is based upon the same design. My opinion is the open systems are better, but if closed works for you then my reasons for reccommending them are moot. As with all decisions in a laboratory, it depends entirely on the needs of the laboratory. Good luck with this decision, Amos Brooks From thisisann <@t> aol.com Sun Dec 30 16:06:50 2007 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Sun Dec 30 16:07:19 2007 Subject: [Histonet] Special Stain Control List Message-ID: <8CA194769F46B68-3A8-402B@webmail-me11.sysops.aol.com> Does anyone have a list of controls to use for Special Stains?? I would like to post one in the lab for my staff and can not find a good list.? I have one for IHC if anyone would like a copy. Thanks, Ann ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From nyilmaz <@t> mersin.edu.tr Mon Dec 31 02:53:26 2007 From: nyilmaz <@t> mersin.edu.tr (Nejat) Date: Mon Dec 31 02:54:42 2007 Subject: [Histonet] Re: IHC stainers Biogenex i6000 References: <20071230182159.72067321C60@mail.mersin.edu.tr> Message-ID: <002e01c84b8a$9b8a95e0$5201a8c0@NEJAT1> Dear Netters, I read a lot of information IHC autostainers here, but, what about Biogenex i6000? We have bought one and waiting for installation. It is a really cheap one. Thanks in advance... Dr. Necat Yilmaz ----- Original Message ----- From: To: Sent: Sunday, December 30, 2007 8:21 PM Subject: Histonet Digest, Vol 49, Issue 31 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: bone holders (Gayle Callis) > 2. Re: Websites to demonstrate IHC methods (Gayle Callis) > 3. RE: IHC stainers (pruegg@ihctech.net) > 4. IHC Stainers (Amos Brooks) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 29 Dec 2007 14:03:06 -0700 > From: "Gayle Callis" > Subject: [Histonet] RE: bone holders > To: "Histonet" > Message-ID: <001001c84a5e$35d59470$6501a8c0@DHXTS541> > Content-Type: text/plain; charset="iso-8859-1" > > With a band saw, using pieces of 2 x 4 soft pine wood should hold the > bones. You don't want the saw blade to hit a metal holder as the blade > may shatter and dulled, but worst of all is the potential for injury to > operator. We used this method to saw through whole lengths of femurs of > calves\(huge!) and other animals when using a meat cutters band saw. It > worked for human femoral heads too. Simple grasp the bone between the > pieces of wood - a nice safe way to prevent losing a finger. You can soak > the wood in bleach later, or simply discard for incineration. The wood is > cheap and doesn't hurt the blade. Try to have more teeth per inch in a > saw blade, you will get smoother, cleaner cuts, We were able to saw with > water running on the blade, but that is not always possible. However it > keeps the bone from being burned by high speed saws. > > Holding the bone this way allowed us to get precise 2 mm to 1 cm slabs for > doing large decalcified bone samples. > > Good luck > > Gayle M. Callis > HT,HTL,MT(ASCP) > Bozeman MT > > > > > > > > > ------------------------------ > > Message: 2 > Date: Sat, 29 Dec 2007 14:09:52 -0700 > From: "Gayle Callis" > Subject: [Histonet] Re: Websites to demonstrate IHC methods > To: "Histonet" > Message-ID: <001701c84a5f$27548c20$6501a8c0@DHXTS541> > Content-Type: text/plain; charset="iso-8859-1" > > Dear K M, > > You can find IHC methods from just about any company selling antibodies > and their ancillary reagents. As for animated IHC, you can find cartoons > for immunostaining on the Vector and DAKO websites, but not animated. > However they are step by step with explanation. Go to www.IHCworld and > look at all the methods found there. If you are learning, this site is > superb for information. > > You can also access the DAKO handbook for immunostaining, and request a > copy of the last edition although I think it is available in pdf form on > the DAKO website. They will send you a hard copy. > > Happy New Year > > Gayle M. Callis > HT/HTL/MT(ASCP) > Bozeman MT > > ------------------------------ > > Message: 3 > Date: Sat, 29 Dec 2007 15:07:35 -0700 > From: > Subject: RE: [Histonet] IHC stainers > To: "'Larry Woody'" , "'Sharon.Davis-Devine'" > , > Message-ID: <005101c84a67$386725b0$0202a8c0@ihctechq9h2qof> > Content-Type: text/plain; charset="us-ascii" > > Sharon, > If you are happy with the Dako, let them offer suggestions to up date your > system, you will certainly have the most flexibility using a dako system > as > it is completely open and much cheaper to use in my experience than being > locked into detection systems for closed systems. For more closed > systems, > I have also heard really good things about Vision BioSystems (now > LeicaBioSystems) machines. Ask Dako and Leica what they can do for you, > make them vie for your business, you will get the best deal if they think > they have to compete. > Patsy > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > email pruegg@ihctech.net > website www.ihctech.net > IHC Resource Group www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry > Woody > Sent: Friday, December 28, 2007 5:34 PM > To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC stainers > > Sharon: > I have used both the Ventana and the Lab Vision, (the one > Dako distributes because the only one Dako makes is called Eridan and it > doesn't work) ,and several others not worth mentioning, but if I had my > choice I would probably have several of each. If there are people with > limited experience and knowledge in the area of IHC, then the Ventana is > along those lines however if you are wanting to work up a novel antibody > from scratch then you need an open platform where you can run an > experiment > and the Lab Vision gives you that freedom. > > "Sharon.Davis-Devine" wrote: > I have recently been given the assignment of implementing a new and > updated IHC system in our Histology department. We currently use a Dako > Autostainer which we have used for several years and needs to be > updated. I have limited in expertise in this are so am asking for all > you Histonetters who have experience with this to give me some advice on > which stainer to purchase. Let me know all of your thoughts both good > and bad please. Thank you so much for your help. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Larry A. Woody > Seattle, Wa. > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Sat, 29 Dec 2007 18:16:49 -0500 > From: "Amos Brooks" > Subject: [Histonet] IHC Stainers > To: histonet@lists.utsouthwestern.edu > Message-ID: > <582736990712291516o423bb64dge16d410c7933e786@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > To delve into the mucky waters of this question is a slippery slope > indeed. It honestly depends upon how much control you want to have (or to > give up!) over the staining process and the chemicals you use. I must say > that the Ventana is a FANTASTICALLY engineered bit of equipment. I would > use > a Ventana in a second if it had the versatility I want in an autostainer. > The ability to heat the slides individually and to stir the reagents is > spectacular! Sadly unless you want to cough up a fortune for the high end > machine, you are left having to use the secondary antibodies and > ancillaries > they provide. I believe, (and I'm sure I'll be corrected if I'm wrong) the > BondX is the same in this respect. > Hearing this, one may think that I would reccommend the DAKO and if > you > read the archives you'd be right. Lately I have been less sure of that! > They > have been working on the Eridan for a long time and I'm beginning to think > it is a lost cause! (Think Vista from Microsoft) The development is long > postponed and some say the company is going in the direction of the above > equipment and attempting to lock users into a secondary detection system. > The Original DAKO is still great and I still reccommend it, but I also > reccommend the Thermo(FisherShandonRichard-AllenLabVision...) It is as, if > not more versatile than the DAKO since it is based upon the same design. > My opinion is the open systems are better, but if closed works for you > then my reasons for reccommending them are moot. As with all decisions in > a > laboratory, it depends entirely on the needs of the laboratory. > > Good luck with this decision, > Amos Brooks > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 49, Issue 31 > **************************************** From awatanabe <@t> tgen.org Mon Dec 31 08:19:39 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Mon Dec 31 08:20:05 2007 Subject: [Histonet] Re: IHC stainers In-Reply-To: <20071230180303.5149156E72@mr1.tgen.org> Message-ID: Hi all, I agree with many of the posters that your first question in deciding on a machine is how much off-line work you want to be involved in. Next you should think about tech experience, how open a machine needs to be for your lab, etc. With that said I work in a research lab and evaluated Ventana, DAKO, Biocare's Nemesis, and the Leica (vision biosystems) BondX machine. We do have a lot of novel antibodies to work up but also do many standards as well. We found that the reagents, platform and userability from the BondX was what we needed. We can adapt all of our protocols to fit on their machine and we found the detection kits to be superb. I did not at first want this machine but I was sold on the staining we were able to get using their reagents and platform. I have been able to adapt all my mouse and rabbit primary antibodies to their detection kits usually pushing out titers and using shorter runs. The machine bakes, dewaxes, antigen retrieves, stains, and counterstains. We really like the capability we have with this machine. We also have had great technical/customer support since we have had the machine. This is just my two cents. Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From gayle.callis <@t> bresnan.net Mon Dec 31 10:23:10 2007 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Dec 31 10:23:09 2007 Subject: [Histonet] Special Stain Control List References: <8CA194769F46B68-3A8-402B@webmail-me11.sysops.aol.com> Message-ID: <000f01c84bc9$6f0ad630$6501a8c0@DHXTS541> Dear Ann, There is one on the IHCworld website, under postive control list. I Iknow Peggy Wenk will have one which she has also published in J of Histotechnology. Histo Logic on Sakura Finetek websit has had lists over the years too. Many histotechnology textbooks will have lists too. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT ----- Original Message ----- From: To: Sent: Sunday, December 30, 2007 3:06 PM Subject: [Histonet] Special Stain Control List > Does anyone have a list of controls to use for Special Stains?? I would > like to post one in the lab for my staff and can not find a good list.? I > have one for IHC if anyone would like a copy. > Thanks, > Ann > ________________________________________________________________________ > More new features than ever. Check out the new AOL Mail ! - > http://webmail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet