From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Aug 1 01:45:19 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Aug 1 01:45:30 2007 Subject: [Histonet] Cytospin - misshapen cells Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EA6B@wahtntex2.waht.swest.nhs.uk> Hi All, I'm having some trouble with cytospins - the cells/nuclei look very misshapen after spinning. I have used a number of different cell lines and have the same problem with them all. I have tried fixing the cells before spinning with 4% PFA in PBS (for this I centrifge the cells, resuspend in PBS, centrifuge, resuspend in PFA for 10min on ice, centrifuge, resuspend in PBS and cytospin). Unfixed cells were cytopsun in media. The cytospin is set to 500rpm for 5min with low acceleration. After spinning I let them air dry (~5min), wash PBS, fix 4% PFA (if not post-fixed), wash PBS and continue with staining. When staining the nuceli just with Dapi I get some very strange looking nuclei, please see some pics on www.histonet.org the file is called Cytospin Dapi.jpg. Is this normal for cytospins? Any ideas? Thanks Sonya Did they look 'normal' before? You mentioned 'cell lines' and from my experience these look very abnormal when 'normal'. I remember carrying out cytospins on 'grown' cells and the longer you 'grew' them the more abnormal they looked. Maybe you're looking at normal. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; If the world seems cold to you, kindle fires to warm it. --Lucy Larcom This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From mlm11 <@t> cornell.edu Wed Aug 1 06:01:48 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Wed Aug 1 06:01:57 2007 Subject: [Histonet] slide carousels Message-ID: <6.2.1.2.2.20070801065654.01f2feb8@postoffice9.mail.cornell.edu> Hello Histonet, 80 -slide carousels up for grabs. I think there are 14 of them. I don't know if they each have their own box. You don't have to take them all. Thanks, Mary Lou From jmahoney <@t> alegent.org Wed Aug 1 07:11:13 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Aug 1 07:11:32 2007 Subject: [Histonet] interesting pictures In-Reply-To: <1341615539.20070730230150@mail.ru> References: <1341615539.20070730230150@mail.ru> Message-ID: <46B032110200003C0001596C@gwia.alegent.org> Wow, don't you just love the net! Thanks for the information Maxim and good luck with your progress. Jan, Omaha, Nebraska, USA >>> Maxim Peshkov 07/30/2007 2:01 PM >>> Dear histonetters! Pictures, which you saw are really. So or approximately so look the majority histo labs in Russia. Our country has also very progressive and rich labs. I belive that now we today have much possibilities for improvement of the work conditions in our labs. You help to us by your advices. It seems that we shall reach the modern level much quicker, than you came hereto its way. The process of the development does not look at condition purse or wit. If something must be developed, it will is developed earlier or later. It is possible that we can turn out to be in side, if we can not participate in this themselves. Thank you all. Maxim Peshkov Russia, Taganrog. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From syedab <@t> totalise.co.uk Wed Aug 1 10:39:24 2007 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Wed Aug 1 10:39:40 2007 Subject: [Histonet] stability of lipids in wax References: <1341615539.20070730230150@mail.ru> <46B032110200003C0001596C@gwia.alegent.org> Message-ID: <004501c7d452$22f903a0$c6c401a3@LENOVO27D521E3> Dear All, Are there any references which show that lipids are stable in wax-embedded tissue? Thanks Anila From edessas <@t> rmy.emory.edu Wed Aug 1 12:10:42 2007 From: edessas <@t> rmy.emory.edu (Evan Dessasau) Date: Wed Aug 1 12:12:04 2007 Subject: [Histonet] Histology duties in necropsy reply to message 7 In-Reply-To: <200707311642.l6VGgCfq021131@mr5.cc.emory.edu> References: <200707311642.l6VGgCfq021131@mr5.cc.emory.edu> Message-ID: <46B0BE92.5050106@rmy.emory.edu> histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: hemoglobin (Cheri Miller) > 2. RE: Fat issues (Cheri Miller) > 3. RE: interesting pictures (Cheri Miller) > 4. RE: Fat issues (Cheri Miller) > 5. RE: Cytospin - misshapen cells (Gayle Callis) > 6. not histology related (well, almost) (Joe Nocito) > 7. Histology duties in necropsy (Wilson, Carol) > 8. stains for myocardial injury (Ford, Judi) > 9. breast (anita dudley) > 10. Re: stains for myocardial injury (Andrea Grantham) > 11. Reading FISH with Bio View (Diane Cadorette-Hall) > 12. Re: breast (LuAnn Anderson) > 13. RE: Cytospin - misshapen cells (Tony Henwood) > 14. copath plus (pereirafamily) > 15. HER2 FISH (Bell, Lynne) > 16. Re: not histology related (well, almost) (Fred Underwood) > 17. RE: Recycling lab equipment? (Garth Jerome) > 18. Re: not histology related (well, almost) (louise renton) > 19. RE: not histology related (well, almost) (Garth Jerome) > 20. Smushed tubing (John MacDougall) > 21. Attn BondMax users-Can I ask a few questions? (Greg Dobbin) > 22. RE: interesting pictures (Edwards, R.E.) > 23. RE: stains for myocardial injury (Bernice Frederick) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 30 Jul 2007 08:39:40 -0500 > From: "Cheri Miller" > Subject: RE: [Histonet] hemoglobin > To: "'Janice Mahoney'" , > , "'Derek Papalegis'" > > Message-ID: <00e301c7d2af$17dfa130$3402a8c0@plab.local> > Content-Type: text/plain; charset="US-ASCII" > > That makes more sense....Investigator = Pathologist ?? You had me worried > for a few. > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > -----Original Message----- > From: Janice Mahoney [mailto:jmahoney@alegent.org] > Sent: Monday, July 30, 2007 7:46 AM > To: histonet@lists.utsouthwestern.edu; Cheri Miller; 'Derek Papalegis' > Subject: RE: [Histonet] hemoglobin > > I think Derek is talking about a request to do a stain by an > investigator, not a lab inspector. > Jan > Omaha > > >>>> "Cheri Miller" 07/30/2007 7:11 AM >>> >>>> > This may be a dumb question, But why would the inspector insist you do > a > hemoglobin stain if it's not part of your testing menu?? What entity > is > inspecting your lab? Just curious, Cheri > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible > for > delivering it to the addressee, you are hereby notified that you are > in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek > Papalegis > Sent: Wednesday, July 25, 2007 10:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] hemoglobin > > Hi All, > An investigator has asked me to stain some sections for hemoglobin. I > have already provided him with an iron stain and he wants to go further > > with it. Can anyone recommend a stain specifically for hemoglobin? I > have found some for hemosiderin but I am unsure as to if they will be > sufficient. If someone could let me know what stain they use and what > > the procedure is I would greatly appreciate it. > > Thanks! > -Derek > > Here at the research facility I work in we share the tissue trimming duties with the two people that work in necropsy. Those two individuals do not do histology. thanks, E-van From bakerj <@t> umich.edu Wed Aug 1 12:46:42 2007 From: bakerj <@t> umich.edu (John Baker) Date: Wed Aug 1 12:54:16 2007 Subject: [Histonet] Thin sectioning bone in PMMA Message-ID: Hello, We are sectioning rat femur embedded in PMMA on the Polycut microtome with tungsten-carbide blade. Does anyone have any suggestions/protocols for getting excellent sections at 5 microns? Are there any tricks to mounting that will help eliminate the wrinkles? We mount and roll on Haupt's coated Superfrost slides, then under a press in a 40 degree C oven overnight to dry. Our sections are good but there is room for improvement. Thanks in advance. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From mlm11 <@t> cornell.edu Wed Aug 1 12:55:58 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Wed Aug 1 12:56:08 2007 Subject: [Histonet] re:carousels Message-ID: <6.2.1.2.2.20070801135447.03f2a8d0@postoffice9.mail.cornell.edu> There only 8 carousels and they are in their own boxes. Will ship anywhere. Thanks. From RSRICHMOND <@t> aol.com Wed Aug 1 12:57:44 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Aug 1 12:57:59 2007 Subject: [Histonet] Re: stains for myocardial injury Message-ID: I remember reading articles about the hematoxylin / basic fuchsin / picric acid stain for recently infarcted myocardium, some time late in the Ordovician Period I think. I've never seen one, but there was a question about the stain on the anatomic pathology board examination (American Board of Pathology) when I took it in November 1971. I remember it because it was the only question on anything contemporary that was on the exam. Gayle Callis, if you post the procedure in electronic form, I'd like to have a copy. I can't remember whether it's done from neutral buffered formalin or whether it requires a special fixative. The Harris (mercuric oxide) hematoxylin could make a difference - it does in the Engel-Cunningham variant of the Gomori trichrome stain, used for frozen sections of skeletal muscle. Bob Richmond Samurai Pathologist Knoxville TN (actually in Harlan KY this week) ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From awatanabe <@t> tgen.org Wed Aug 1 13:06:06 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Wed Aug 1 13:06:16 2007 Subject: [Histonet] Re: Attn BondMax users In-Reply-To: <20070801170209.871D42005007@mr1.tgen.org> Message-ID: I have had the Bond autostainer for almost 2 years and find it a great machine. We are a research group and love the openness it allows us. We found the staining to be very reproducible. Most antibodies we can push the dilution out beyond the recommendations. We use the refine kit and the AP kit and find that we get good results with both. I hope this helps. Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From mwstarbu <@t> mdanderson.org Wed Aug 1 13:10:40 2007 From: mwstarbu <@t> mdanderson.org (mwstarbu@mdanderson.org) Date: Wed Aug 1 13:18:25 2007 Subject: [Histonet] Thin sectioning bone in PMMA Message-ID: We apply a 50% butyl glycol, 35% ethanol solution to the sections. The application is as follows. We apply a liberal amount with a small paint brush and let dry 5 -10 minutes under a fume hood. Apply again and use downward pressure with the brush to remove wrinkles. During the 2nd application you will notice the sections are much more pliable enableing you to easily remove large wrinkles. We then cover the slides with plastic strips, press and dry at 55 degrees C overnnight Mike From nmeres <@t> gmu.edu Wed Aug 1 13:51:36 2007 From: nmeres <@t> gmu.edu (Norman Meres) Date: Wed Aug 1 13:51:56 2007 Subject: [Histonet] Cryostat issues with lobster tissues Message-ID: <28C1C58D-C6BD-4992-BAF6-E21E38242EE5@gmu.edu> Hello: I am adapting a histochemical technique for sectioning lobster epidermis and staining for enzyme activity. I have been trying to find an ideal freezing temperature for the cryostat. The technique I am adapting was originally developed for scyphozoans, and calls for freezing temp of ?30 C. This appears to be too cold, as the tissue just crumbles the blade crosses it. I have tried ?20 C, and this appears to be better, but I was wondering if anyone has experience with freezing arthropod or crustacean tissues, and might have a suggested temp. Thanks, Norman Begin forwarded message: > From: mwstarbu@mdanderson.org > Date: August 1, 2007 2:10:40 PM EDT > To: Histonet@pathology.swmed.edu, histonet- > bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Thin sectioning bone in PMMA > > We apply a 50% butyl glycol, 35% ethanol solution to the > sections. The > application is as follows. We apply a liberal amount with a small > paint > brush and let dry 5 -10 minutes under a fume hood. Apply again and > use > downward pressure with the brush to remove wrinkles. During the 2nd > application you will notice the sections are much more pliable > enableing > you to easily remove large wrinkles. We then cover the slides with > plastic strips, press and dry at 55 degrees C overnnight > > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Frei <@t> sial.com Wed Aug 1 14:30:04 2007 From: Mark.Frei <@t> sial.com (Mark Frei) Date: Wed Aug 1 14:30:29 2007 Subject: Subject: [Histonet] Enzyme staining Question In-Reply-To: <200708011707.l71H7EYb014246@stlspfw1.sial.com> Message-ID: Hi Judi, I have found the diazonium-naphthol deposits to be quite stable. I've performed cytochemistry assays for many years and the highly colored deposits of the specific esterase stain don't fade appreciably for months. I've even used slides like smears and viewed under oil immersion. The mineral oil doesn't seem to react with the stain either. I just wipe the excess oil off the slide when finished. I've come back later and the stain hasn't faded. You are correct to use aqueous mounting media. Xylene based mounting media will cause the stain to fade away pretty quick. For those that wish a permanent mounting media, Crystal Mount works well without fading the stain. Mark Frei MT(ASCP) Sigma-Aldrich Corporation 3050 Spruce Street St. Louis, MO 63103 (314) 286-8080 Message: 8 Date: Tue, 31 Jul 2007 14:53:18 -0700 From: "Ford, Judi" Subject: [Histonet] Enzyme staining Question To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Everyone, First I want to thank you all for the information on the different stains for the myocardial ischemia. We have a plan to work with now and alternatives if the pathologist asks for more work. My next question involves enzyme staining. I am doing a Naphthol AS-D Chloroacetate Esterase procedure that requires aqueous mounting media. This may be a dumb question but I was wondering if its better to do the procedure and examine right away or can the finished slide be examined at a later (day or two) without any fading artifact happening? From LSebree <@t> uwhealth.org Wed Aug 1 16:17:31 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Aug 1 16:31:19 2007 Subject: [Histonet] Re: stains for myocardial injury In-Reply-To: Message-ID: Bob, I used to do HBFP for MIs in the Ordovician Period. I believe it was developed at the Mayo clinic in that same era but fell out of favor when it was determined that one could easily manipulate the outcome to be positive or negative by the differentiation step. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Wednesday, August 01, 2007 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: stains for myocardial injury I remember reading articles about the hematoxylin / basic fuchsin / picric acid stain for recently infarcted myocardium, some time late in the Ordovician Period I think. I've never seen one, but there was a question about the stain on the anatomic pathology board examination (American Board of Pathology) when I took it in November 1971. I remember it because it was the only question on anything contemporary that was on the exam. Gayle Callis, if you post the procedure in electronic form, I'd like to have a copy. I can't remember whether it's done from neutral buffered formalin or whether it requires a special fixative. The Harris (mercuric oxide) hematoxylin could make a difference - it does in the Engel-Cunningham variant of the Gomori trichrome stain, used for frozen sections of skeletal muscle. Bob Richmond Samurai Pathologist Knoxville TN (actually in Harlan KY this week) ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judi.ford <@t> roche.com Wed Aug 1 16:37:01 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Wed Aug 1 16:37:12 2007 Subject: [Histonet] Gomori aldehyde/trichrome stain Message-ID: Thank you all so much for the suggestions you've been sending. I really appreciate all the info. Its been quite helpful. Sooooo, here's one more question: Has anyone done a combination of Gomori's aldehye fuchsin and Gomori's trichrome stains? If so, do you have a protocol you could send me? We've had no luck searching for this so far. Hope to hear some great suggestions again. Cheers! Judi Roche Palo Alto Palo Alto, CA From djemge <@t> aol.com Wed Aug 1 17:01:33 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Wed Aug 1 17:01:43 2007 Subject: [Histonet] Yasue's Silver Nitrate-Rubeanic Acid Method for Calcium Oxalate Message-ID: <8C9A29EA82B3D21-884-7D33@mblk-d14.sysops.aol.com> Does anyone have the protocol for Yasue's Silver Nitrate Rubeanic Acid method. One of the researchers is doing a calcium oxalate study. I have already given him Pizzolato's, Von Kossa, and Alizarin Red for comparisons. I have heard that the Yasue's method is much more specific. Thanks, Donna Donna Emge, HT(ASCP) Northwestern University Feinburg School of Medicine 303 E Superior St. Lurie 7-220 Chicago, IL 60611 d-emge@northwestern.edu ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From AnthonyH <@t> chw.edu.au Wed Aug 1 18:17:48 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 1 18:18:48 2007 Subject: [Histonet] Yasue's Silver Nitrate-Rubeanic Acid Method for CalciumOxalate Message-ID: This is the one we have used: Yasue's Method for Calcium Oxalate Calcium oxalate can be demonstrated in several organs in patients suffering from hereditary or acquired oxalosis. Calcium oxalate is also common in the thyroid in cases of nodular goitre. Oxalates have been identified in giant cells in various granulomatous lesions such as skin granuloma, tuberculous or chronic granulomatous salpingitis, and sarcoid granuloma (Katoh et al 1993). Oxalosis is also associated with aspergillosis Under high power calcium oxalate appears as colourless crystals that exhibit brilliant birefringence under polarised light. They stain black-brown with Yasue's method (Yasue 1969), where as they do not stain with von Kossa's or alizarin red S (Katoh et al 1993). Yasue (1969) Acta Histochem Cytochem 2:83-95. Katoh et al (1993) Am J Surg Pathol 17(7):698-705. Fixation: 10% buffered formalin. Microtomy: 5?m paraffin sections. Solutions 1. 5% Acetic Acid 2. 5% Potassium Hydroxide 3. 5% Silver Nitrate 4. Rubeanic Acid Solution: Prepare a saturated solution of rubeanic acid in 100ml of 70% ethanol Add 2 drops of concentrated ammonium 5. 2% Methyl Green 6. 50% ethanol Method: 1. Dewax and Hydrate sections 2. Treat with 5% acetic acid 30min. 3. Wash in water 4. Place in 5% Potassium Hydroxide 30min. 5. Wash in water 6. Rinse in distilled water 7. Place in 5% silver nitrate 20min. 8. Rinse in distilled water 9. Place in Rubeanic acid solution for 1min 10. Rinse in 50% ethanol 11. Rinse in water. 12. Counterstain in 2% Methyl Green 2min. 13. Dehydrate, clear and mount Results: Calcium Oxalate Brown/black Nuclei Green. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of djemge@aol.com Sent: Thursday, 2 August 2007 8:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Yasue's Silver Nitrate-Rubeanic Acid Method for CalciumOxalate Does anyone have the protocol for Yasue's Silver Nitrate Rubeanic Acid method. One of the researchers is doing a calcium oxalate study. I have already given him Pizzolato's, Von Kossa, and Alizarin Red for comparisons. I have heard that the Yasue's method is much more specific. Thanks, Donna Donna Emge, HT(ASCP) Northwestern University Feinburg School of Medicine 303 E Superior St. Lurie 7-220 Chicago, IL 60611 d-emge@northwestern.edu ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From dcojita <@t> tampabay.rr.com Wed Aug 1 18:36:07 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Aug 1 18:38:48 2007 Subject: [Histonet] breast In-Reply-To: Message-ID: We have been thinking about having a grosser "on-call" for these specimens. I realize this would not be possible for every institution. I would be interested in ideas regarding this situation as well. What if a breast came in on a Friday which happened to be Christmas day, and didn't get grossed until Monday. Quite possible... especially in an independent laboratory. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Monday, July 30, 2007 5:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] breast I asked this question a few weeks ago and got 1 answer, what is everyone doing about the new cap requirements about fixing breast tissue no more that 48 hrs if a her2 is to be done? we do not work on the week end and friday that would be a problem if we had a breast. thanks for your input. anita _________________________________________________________________ See what you're getting into.before you go there. http://newlivehotmail.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Aug 2 01:54:29 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Aug 2 01:54:37 2007 Subject: [Histonet] stability of lipids in wax Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EA8F@wahtntex2.waht.swest.nhs.uk> Feustel and Geyer (Acta Histochem. (Jena), 25: 219, 1966)suggest that phospholipids can be retained after acrolein fixation. Loss of polar lipids occurs after fixation in formalin. Baker and Elbers and Adams noted retention of lipids in tissue after calcium was added to formalin; cationic bridges, it is suggested, are formed between the polar groups of phospholipids , calcium and other tissue constituents. Hydrophilic lipids can be retained by using osmium tetraoxide (Wigglesworth, Proc. R. Soc Lond. (Biol) 147:185, 1957) or by using potassium dichromate (Eftman J.Histochem Cytochem, 2:1 1954) Both of these oxidants produce cyclic metal esters bridging a former double bond. Elleder and Lojda (Histochemie, 34:143, 1973) detected significant amounts of sphingomyelin, cerebrosides, sulphatides and gangliosides after formalin fixation, ethanol acetone and xylene, with paraffin embedding. Once again, the God that is Lillie explains much. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Real integrity is doing the right thing, knowing that nobody's going to know whether you did it or not. --Oprah Winfrey his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From nickandmanda <@t> paradise.net.nz Thu Aug 2 02:48:52 2007 From: nickandmanda <@t> paradise.net.nz (Nick and Amanda) Date: Thu Aug 2 02:49:04 2007 Subject: [Histonet] To Albert C re: Fatty tissue blowing up on waterbath Message-ID: <003a01c7d4d9$91dba070$c8084979@bow1> Albert, I was shown a simple trick years ago and have introduced it to many people since. Simply one single drop of household detergent (not budget, it doesn't work!) onto the waterbath changes the surface tension of the water and holds your fatty sections together.. Hope this is helpful! we use it all the time.. Manda of Wellington,NZ From syedab <@t> totalise.co.uk Thu Aug 2 03:26:00 2007 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Thu Aug 2 03:26:11 2007 Subject: [Histonet] stability of lipids in wax References: <86ADE4EB583CE64799A9924684A0FBBF0222EA8F@wahtntex2.waht.swest.nhs.uk> Message-ID: <003501c7d4de$c1fd2710$c6c401a3@LENOVO27D521E3> Dear Kemlo, Many thanks, that is soooo helpful! I did do a pubmed search and google search etc, but got befuddled by the number of hits and it's better just to ask someone usually. Many thanks!! Anila ----- Original Message ----- From: "Kemlo Rogerson" To: "Anila Syed" ; Sent: Thursday, August 02, 2007 7:54 AM Subject: RE: [Histonet] stability of lipids in wax Feustel and Geyer (Acta Histochem. (Jena), 25: 219, 1966)suggest that phospholipids can be retained after acrolein fixation. Loss of polar lipids occurs after fixation in formalin. Baker and Elbers and Adams noted retention of lipids in tissue after calcium was added to formalin; cationic bridges, it is suggested, are formed between the polar groups of phospholipids , calcium and other tissue constituents. Hydrophilic lipids can be retained by using osmium tetraoxide (Wigglesworth, Proc. R. Soc Lond. (Biol) 147:185, 1957) or by using potassium dichromate (Eftman J.Histochem Cytochem, 2:1 1954) Both of these oxidants produce cyclic metal esters bridging a former double bond. Elleder and Lojda (Histochemie, 34:143, 1973) detected significant amounts of sphingomyelin, cerebrosides, sulphatides and gangliosides after formalin fixation, ethanol acetone and xylene, with paraffin embedding. Once again, the God that is Lillie explains much. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Real integrity is doing the right thing, knowing that nobody's going to know whether you did it or not. --Oprah Winfrey his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From mpence <@t> grhs.net Thu Aug 2 08:38:30 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 2 08:38:44 2007 Subject: [Histonet] To Albert C re: Fatty tissue blowing up on waterbath In-Reply-To: <003a01c7d4d9$91dba070$c8084979@bow1> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C69A@IS-E2K3.grhs.net> What does this do to tissue you are going to use for IHC? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nick and Amanda Sent: Thursday, August 02, 2007 2:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] To Albert C re: Fatty tissue blowing up on waterbath Albert, I was shown a simple trick years ago and have introduced it to many people since. Simply one single drop of household detergent (not budget, it doesn't work!) onto the waterbath changes the surface tension of the water and holds your fatty sections together.. Hope this is helpful! we use it all the time.. Manda of Wellington,NZ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Thu Aug 2 09:01:24 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Aug 2 09:01:34 2007 Subject: [Histonet] To Albert C re: Fatty tissue blowing up on waterbath In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C69A@IS-E2K3.grhs.net> Message-ID: <36159.8683.qm@web53609.mail.re2.yahoo.com> Put the section on the slide dry and then lower the slide with the tissue on it into the water bath as evenly as you can. When the water comes onto the slide and under the tissue raise it out of the water immediately. Good Luck! Mike Pence wrote: What does this do to tissue you are going to use for IHC? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nick and Amanda Sent: Thursday, August 02, 2007 2:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] To Albert C re: Fatty tissue blowing up on waterbath Albert, I was shown a simple trick years ago and have introduced it to many people since. Simply one single drop of household detergent (not budget, it doesn't work!) onto the waterbath changes the surface tension of the water and holds your fatty sections together.. Hope this is helpful! we use it all the time.. Manda of Wellington,NZ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From derek.papalegis <@t> tufts.edu Thu Aug 2 09:28:04 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Thu Aug 2 09:28:14 2007 Subject: [Histonet] trichrome problems Message-ID: <46B1E9F4.9010304@tufts.edu> Hi Everyone, I am having trouble with the tissue staying on the slide when doing a trichrome stain. I currently use the Richard Allen super up-rite slides. I found that the tissue came off the slide after I put it in the heated bouin's solution for an hour. I tried to leave it in room temp bouin's overnight instead of using it heated and I found that some tissue has stayed on but some has still fallen off. I have tried both leaving slides out to dry overnight as well as cutting and staining on the same day. Does anyone have any ideas of how I can solve this problem? Thanks, Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From sbledsoe <@t> iupui.edu Thu Aug 2 09:31:12 2007 From: sbledsoe <@t> iupui.edu (Sharon Bledsoe) Date: Thu Aug 2 09:31:23 2007 Subject: [Histonet] Yasue's Silver Nitrate-Rubeanic Acid Method for Calcium Oxalate In-Reply-To: <8C9A29EA82B3D21-884-7D33@mblk-d14.sysops.aol.com> References: <8C9A29EA82B3D21-884-7D33@mblk-d14.sysops.aol.com> Message-ID: Donna, Our Lab has been using the Yasue Stain for years now. Yasue Method of Staining Calcium Histochemical Identification of Calcium Oxalate Acta Histochem, Cytochem, Vol 2(3), 1969, pp 83-95. This is a metal substitution method for calcium histochemistry. Metal substitution depends on a reaction to substitute calcium, which is bound to anion, with other heavy metals, and therefore it does not detect calcium cation directly but such anions as carbonate, phosphate, sulfate and oxalate. Rubeanic acid reacts with silver and some other metals to form a colored double salt. A brownish-black insoluble double salt is produced with a reaction of rubeanic acid and silver, the reaction is very sensitive and specific if there are no other salts. Slightly deposited silver particles on the surface of crystals of calcium oxalate with a low ability to react are assumed to be blackened with rubeanic acid and its sensitivity as well as localization are good enough to demonstrate calcium oxalate. This reaction can not be taken as a specific one for calcium oxalate. Calcium Oxalate crystals are demonstrated histochemically when optical characteristics (birefringence), hematoxylin and eosin staining (lack of), solubility test (soluble in 2% HCl, not soluble in 5% acetic acid), microincineration method and gypsum formation reaction are combined. METHOD: 1. Removal of Calcium Phosphate and Calcium Carbonate soak in 5% Acetic Acid for 30 minutes. 2. Rinse in running distilled water for 3 minutes 3. Immerse in 5% Aqueous Silver Nitrate (AgNO3) 10 - 20 minutes. (12 minutes seemed to work best for the rat kidneys--15 minutes left a film on top of the tissue that was probably from the Rubeanic Acid in the next step). 4. Rinse in running distilled water for 3 minutes 5. Add 2 drops of strong Ammonia Water (10 %) to a Coplin jar filled with saturated Rubeanic Acid. Immerse slides for about 1 minute. 6. Rinse in 50 % EtOH followed by distilled water. 7. Counterstain with Methyl Green or Safranin O. 8. Dehydrate, clear and mount Calcium deposits are colored dark brown or black. SOLUTIONS: 5% AgNO3 (5 gm / 100 ml of ddH2O) Saturated Rubenic Acid About 300 mg / 100 ml of 70% EtOH Safranin O: Heat water to about 80 degrees, add about 4 gm / 100 ml. Stain is very strong.. needed to dilute.. took about 2 seconds to stain. We have some nice images of the staining in the following: Evan, AP et al. 2003, Randall's plaque of patients with nephrolithiasis begins in basement membranes of thin loops of Henle. Journal of Clinical Investigation.111:607-616. Evan, AP et al. 2005. Crystal-associated nephropathy in patients with brushite nephrolithiasis. Kidney International 67:576-591 Evan, AP et al. 2005. Apatite plaque particles in inner medulla of kidneys of calcium oxalate stone formers: Osteopontin localization. Kidney International 68:145-154 Hope this helps, Sharon Bledsoe Research Analyst Indiana University, School of Medicine Department of Anatomy & Cell Biology MS 5055P, 635 Barnhill Drive Indianapolis, IN 46202 sbledsoe@iupui.edu ______________________________________________________________________________ At 6:01 PM -0400 8/1/07, djemge@aol.com wrote: >Does anyone have the protocol for Yasue's Silver Nitrate Rubeanic >Acid method. One of the researchers is doing a calcium oxalate >study. I have already given him Pizzolato's, Von Kossa, and Alizarin >Red for comparisons. I have heard that the Yasue's method is much >more specific. >Thanks, Donna > >Donna Emge, HT(ASCP) >Northwestern University >Feinburg School of Medicine >303 E Superior St. Lurie 7-220 >Chicago, IL 60611 >d-emge@northwestern.edu > >________________________________________________________________________ >AOL now offers free email to everyone. Find out more about what's >free from AOL at AOL.com. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Thu Aug 2 09:54:06 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Aug 2 09:54:16 2007 Subject: [Histonet] Re: Trichrome Problems Message-ID: Derek, Our tissue are collected onto charged slides (Fisherbrand SuperFrost) and allowed to adhere overnight on the slide warmer at ~37C. I had the very same problem when I was trichrome staining. I found that 1hr in the heated Bouins fixative caused the tissue to come loose from the slide. I had much better luck with the overnight fix at room temperature and I haven't had any significant problems since I started using the O/N post-fix. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From MSHERWOOD <@t> PARTNERS.ORG Thu Aug 2 09:55:41 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Aug 2 09:55:52 2007 Subject: [Histonet] trichrome problems In-Reply-To: <46B1E9F4.9010304@tufts.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D970DC831@PHSXMB30.partners.org> We routinely leave our slides in a 60 degree oven overnight before staining. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Derek Papalegis Sent: Thursday, August 02, 2007 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] trichrome problems Hi Everyone, I am having trouble with the tissue staying on the slide when doing a trichrome stain. I currently use the Richard Allen super up-rite slides. I found that the tissue came off the slide after I put it in the heated bouin's solution for an hour. I tried to leave it in room temp bouin's overnight instead of using it heated and I found that some tissue has stayed on but some has still fallen off. I have tried both leaving slides out to dry overnight as well as cutting and staining on the same day. Does anyone have any ideas of how I can solve this problem? Thanks, Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From jwatson <@t> gnf.org Thu Aug 2 11:38:40 2007 From: jwatson <@t> gnf.org (James Watson) Date: Thu Aug 2 11:39:00 2007 Subject: [Histonet] Histology job in San Diego California Message-ID: Histonetters, GNF a research foundation part of Novartis Pharmaceuticals is expanding its histology department. GNF does a wide variety of research from genomics to drug discovery. We are presently looking for an entry level Histology technician that is energetic and willing to learn. We do manual histochemical staining (yes, we still make all our own stain solutions) and are fully automated in the area of immunohistochemistry, In-situ Hybridization, slide scanning, and image analysis. As an added bonus we are ? mile from the beach and we have great weather here in San Diego. Job Summary Performs routine, special staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications Associate's degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. Acquiring the American Society of Clinical Pathologist (ASCP) certification as a Histology Technician within one year will be required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. Experience An entry level technician is acceptable. We will train the employee in animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs microtomy on rotary microtome, cryostat, and be able to learn to use a sliding microtome. 4. Prepares dyes and solutions in order to perform special or complex procedures. 5. Have the background to do basic histochemical stains and enzymatic histochemical stains. Have the background to learn Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. The Genomics Institute of the Novartis Research Foundation (GNF), located in the Torrey Pines area of San Diego, CA, is funded by the Novartis Research Foundation and dedicated to the development and application of new methods and techniques for genome-wide biological discovery and biomedical research. GNF provides a unique and challenging opportunity to combine exploratory biomedical research with pharmaceutical drug development in a highly interactive, multidisciplinary environment and state-of-the-art facilities. GNF offers excellent compensation and a great benefits package. Visit our website at www.gnf.org EOE Please submit your CV and any supporting documents to: Genomics Institute of the Novartis Research Foundation Job Code: JW7-26 10675 John Jay Hopkins Drive San Diego, CA 92121 Fax: 858/812-1670, or submit online to jobs@gnf.org (subject line must include JW7-26) James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From innvx <@t> sbcglobal.net Thu Aug 2 11:56:54 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Thu Aug 2 11:57:12 2007 Subject: [Histonet] Animal IHC Staining workshop/ Duke University Message-ID: <375485.40572.qm@web82004.mail.mud.yahoo.com> Innovex will be conducting Animal Stat IHC staining at Duke university in Durham, North Carolina on September 11th at 9-12. This is a wet workshop and it is an educational workshop that covers both basic and advanced topics for staining animal tissues in 2 hours. This workshop is CEU approved. The space is very limited to 24 people only , Reserves your space immediately. contact Innovex at 1-800-622-7808 or register on line at innvx.com From brett_connolly <@t> merck.com Thu Aug 2 12:41:36 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Aug 2 12:42:15 2007 Subject: [Histonet] Bond maX price? Message-ID: <63EA0607835FBA4689CEA9EA8B4826925C55E4@usctmx1141.merck.com> Can any recent purchasers of a Bond maX IHC stainer please give me a ball park price for this instrument via private e-mail? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From brett_connolly <@t> merck.com Thu Aug 2 12:46:14 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Aug 2 12:46:54 2007 Subject: [Histonet] anti- phosphatidylserine species crossreactivity question Message-ID: <63EA0607835FBA4689CEA9EA8B4826925C55E7@usctmx1141.merck.com> Does anyone know if the anti-phosphatidylserine [4B6] Moab offered by Abcam and others crossreacts with rat or rabbit? Thanks! Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From Shirley_PHUA <@t> hsa.gov.sg Thu Aug 2 13:01:05 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Aug 2 13:03:04 2007 Subject: [Histonet] Shirley Phua is away on 02 Aug 2007 (Thurs) afternoon. Message-ID: I will be out of the office from 02-08-2007 to 03-08-2007. I'll be away on 02 Aug 2007 (Thursday). I'll be back on 03/08/2007 (Friday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From histology <@t> gradymem.org Thu Aug 2 13:12:11 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Thu Aug 2 13:12:36 2007 Subject: [Histonet] Re: stains for myocardial injury In-Reply-To: References: Message-ID: Here is a copy of the procedure we used to do. Angie MYOCARDIAL ISCHEMIA STAIN (Hematoxylin-Basic Fuchsin-Picric Acid Method) PRINCIPLE: The H & E method does not reveal unequivocal morphologic changes in cases of myocardial infarction until 15 to 20 hours have elapsed after the onset of the injury. The HBFP technique detects early changes of myocardial infarction by coloring affected fibers red. Normal and frankly ischemic myocardium colors yellow. The differential staining results are believed to be caused by an unstable protein complex present in the early phases of myocardial ischemia. SPECIMEN: Any well fixed tissue (10% neutral buffered formalin) may be used. Paraffin embedded tissue is sectioned at 4 microns. REAGENTS: Solution A Alum hematoxylin. Ammonium aluminum sulfate 6 gm Hematoxylin 0.5 gm Yellow mercuric oxide 0.25 gm Mix these 3 ingredients in 70ml of distilled water. Boil 10 minutes. Cool and add 30 ml of glycerol and 4 ml of glacial acetic acid. Filter before use. Solution B Basic fuchsin This is a 0.1% solution in distilled water. Solution C Picric acid This is a 0.1% solution in absolute acetone. STAINING PROCEDURE: 1. Deparaffinize and hydrate to distilled water. 2. Stain in solution A for 10 seconds. 3. Wash in running tap water for 5 minutes. 4. Stain in solution B for 3 minutes. 5. Rinse briefly (5-10 seconds) in distilled water. 6. Rinse briefly (5-10 seconds) in absolute acetone. 7. Differentiate in solution C until the red color ceases to run off the section--usually about 20 seconds . 8. Rinse briefly (5-10 seconds) in absolute acetone. 9. Clear in Americlear. 10. Mount with Mounting Medium. RESULTS: Acutely ischemia myocardium--crimson red Normal myocardium--light brown Nuclei--blue-purple Red blood cells, fibrin, plasma, proteins, elastic fibers, collagen--red Note: The irregular fuchsin staining of myocardial cells along the edge of the section is considered an artifact. REFERENCES: Sheehan, Dezna C.: Theory & Practice of Histotechnology. Second Edition, 1980, C. B. Mosby Company. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: RSRICHMOND@aol.com Date: Wednesday, August 1, 2007 1:37 pm Subject: [Histonet] Re: stains for myocardial injury To: histonet@lists.utsouthwestern.edu > I remember reading articles about the hematoxylin / basic fuchsin > / picric > acid stain for recently infarcted myocardium, some time late in > the Ordovician > Period I think. > > I've never seen one, but there was a question about the stain on > the anatomic > pathology board examination (American Board of Pathology) when I > took it in > November 1971. I remember it because it was the only question on > anything > contemporary that was on the exam. > > Gayle Callis, if you post the procedure in electronic form, I'd > like to have > a copy. I can't remember whether it's done from neutral buffered > formalin or > whether it requires a special fixative. The Harris (mercuric > oxide) hematoxylin > could make a difference - it does in the Engel-Cunningham variant > of the > Gomori trichrome stain, used for frozen sections of skeletal > muscle. > > Bob Richmond > Samurai Pathologist > Knoxville TN (actually in Harlan KY this week) > > > > ************************************** > Get a sneak peek of the all-new AOL at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amylee779 <@t> yahoo.com Thu Aug 2 14:27:25 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Aug 2 14:27:33 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? Message-ID: <708930.69276.qm@web38007.mail.mud.yahoo.com> Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From rjbuesa <@t> yahoo.com Thu Aug 2 14:34:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 2 14:34:19 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? In-Reply-To: <708930.69276.qm@web38007.mail.mud.yahoo.com> Message-ID: <695543.61743.qm@web61216.mail.yahoo.com> Amy: We ran a lab in a teaching hospital with always 5 or more pathology residents. Under those conditions, and for teaching and research purposes, we used to keep our blocks for 9 years (that was our disposable space for blocks cabinets) and the slides for ever. There were blocks used for consult or relating to legal cases that were also kept for ever. Each State has particular rules about minimum periods to retain blocks and slides. Ren? J. Amy Lee wrote: Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From jnocito <@t> satx.rr.com Thu Aug 2 14:52:48 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 2 14:53:08 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? References: <708930.69276.qm@web38007.mail.mud.yahoo.com> Message-ID: <001901c7d53e$b5660840$d49eae18@yourxhtr8hvc4p> Amy, if you are a clinical lab and not research, CAP requires 10 years on blocks, slides and now positive control slides, two years on QC records, log books and requisitions. JTT ----- Original Message ----- From: "Amy Lee" To: "histonet" Sent: Thursday, August 02, 2007 2:27 PM Subject: [Histonet] How many years you keep paraffin blocks and slides? > Hello histonetters, > > Due to the limited space of my lab I need to discard some blocks and > slides. I am thinking setting a "rule" about how long I can keep paraffin > blocks and slides. I need to know how other people do it. Could you share > your "rules"? > > Thanks in advance, > Amy > > > --------------------------------- > Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's > on, when. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Aug 2 14:59:39 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 2 14:59:57 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? In-Reply-To: <708930.69276.qm@web38007.mail.mud.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6A0@IS-E2K3.grhs.net> We follow CAP guidelines. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Thursday, August 02, 2007 2:27 PM To: histonet Subject: [Histonet] How many years you keep paraffin blocks and slides? Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Thu Aug 2 15:05:10 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Thu Aug 2 15:05:27 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? In-Reply-To: <708930.69276.qm@web38007.mail.mud.yahoo.com> References: <708930.69276.qm@web38007.mail.mud.yahoo.com> Message-ID: Forever At 02:27 PM 8/2/2007, Amy Lee wrote: >Hello histonetters, > > Due to the limited space of my lab I need to discard some blocks > and slides. I am thinking setting a "rule" about how long I can > keep paraffin blocks and slides. I need to know how other people do > it. Could you share your "rules"? > > Thanks in advance, > Amy > > >--------------------------------- >Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see >what's on, when. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Thu Aug 2 15:24:00 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Aug 2 15:24:25 2007 Subject: [Histonet] West Nile Virus antibody question Message-ID: For those who are performing West Nile Vrus (wnv) testing, using IHC, especially with a BenchmarkXT, on birds, what antibodies are you using? >From what companies? At what dilutions? I recently purchased from abcam and I have not a successful staining run yet. I have tried 4 different dilutions. I would truly appreciate suggestions. From Herrick.James <@t> mayo.edu Thu Aug 2 15:27:05 2007 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Thu Aug 2 15:27:15 2007 Subject: [Histonet] Von Kossa Stain Message-ID: <572057D3BDD52A46BD05BC6DA50686110CAE9A@MSGEBE22.mfad.mfroot.org> Hi all, Does anyone have a Von Kossa protocol that works well with MMA embedded specimen? We are trying to stain for osteoblasts and any related calcium salts. Your help is much appreciated. Thanks. Jim From jcresor <@t> lcpath.com Thu Aug 2 16:17:39 2007 From: jcresor <@t> lcpath.com (Jennifer Cresor) Date: Thu Aug 2 16:17:48 2007 Subject: [Histonet] acid alcohol with immunos Message-ID: <2b2c74398f67d648851ed09dd952d42e@mail2.lcpath.com> Hello all, I have been told in the past to NOT use acid alcohol to differentiate the hematoxylin on immuno stains and only to use a progressive hematoxylin staining method. I do not remember the reason why. Do any of you have experiences and opinions on this subject? Is there a chance to harm immuno stains using acid alcohol and if so why? Thank you for your time. Jennifer Longview, Wa. jcresor@lcpath.com From smclaugh <@t> fhcrc.org Thu Aug 2 16:51:45 2007 From: smclaugh <@t> fhcrc.org (McLaughlin, Sharon R) Date: Thu Aug 2 16:51:52 2007 Subject: [Histonet] Per-diem work in Seattle area Message-ID: <9214508BF36C4D4697A25230CE97EE41021F7C6A@groucho.fhcrc.org> Hi, I'm looking for extra work in Histology. If there is anyone out there needing a Histologist in the Seattle area let me know. I can work week-ends or evenings. I would be willing to do cutting; special staining; embedding; etc. Sharon McLaughlin 206-667-6115 From mtarango <@t> nvcancer.org Thu Aug 2 16:52:12 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Aug 2 16:52:36 2007 Subject: [Histonet] CD26 antibody...where to buy? Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6D95@NVCIEXCH02.NVCI.org> Hello All, Does anyone know where to get an antibody against CD26 that works on formalin-fixed paraffin-embedded tissue sections? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From AnthonyH <@t> chw.edu.au Thu Aug 2 17:07:40 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Aug 2 17:08:56 2007 Subject: [Histonet] acid alcohol with immunos Message-ID: The only reason would be if the enzyme reaction product is soluble in ethanol (eg AEC) or acidic solutions. But isn't Mayers acidic? The reason could possibly be that Mayers, being progressive, can be used as a fainter nuclear stain than the more stronger Harris's (ie stain in Mayers for a shorter time than that usually recommended). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Cresor Sent: Friday, 3 August 2007 7:18 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] acid alcohol with immunos Hello all, I have been told in the past to NOT use acid alcohol to differentiate the hematoxylin on immuno stains and only to use a progressive hematoxylin staining method. I do not remember the reason why. Do any of you have experiences and opinions on this subject? Is there a chance to harm immuno stains using acid alcohol and if so why? Thank you for your time. Jennifer Longview, Wa. jcresor@lcpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From dellav <@t> musc.edu Thu Aug 2 17:29:00 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Aug 2 17:29:14 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? In-Reply-To: <708930.69276.qm@web38007.mail.mud.yahoo.com> References: <708930.69276.qm@web38007.mail.mud.yahoo.com> Message-ID: <7F6B678A32B0564196138E6B3101996A8AA356@EVS1.clinlan.local> I agree with Rene. If you are a clinical lab be sure to check any state requirements that may exist. For example, while CAP requires 10 years, New York State requires 20 years (at least they did when I last lived there). Here, we keep blocks indefinitely (more than 20 years) Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Thursday, August 02, 2007 3:27 PM To: histonet Subject: [Histonet] How many years you keep paraffin blocks and slides? Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Aug 2 17:43:38 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Aug 2 17:43:44 2007 Subject: [Histonet] acid alcohol with immunos In-Reply-To: <2b2c74398f67d648851ed09dd952d42e@mail2.lcpath.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CC0@LSRIEXCH1.lsmaster.lifespan.org> Well of course it depends on what your chromogen is. But if you are using peroxidase/DAB, then acid alcohol will certainly have no effect on the immuno stain. I have taken coverslips off peroxidase-stained slides, completely removed the hematoxylin with acid alcohol, and restained with a different nuclear stain like methyl green. The immuno stain was unaffected. From dcojita <@t> tampabay.rr.com Thu Aug 2 18:34:59 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Thu Aug 2 18:37:37 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? In-Reply-To: <001901c7d53e$b5660840$d49eae18@yourxhtr8hvc4p> Message-ID: Florida mandates 10 years for slides and blocks (same as CAP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, August 02, 2007 3:53 PM To: Amy Lee; histonet Subject: Re: [Histonet] How many years you keep paraffin blocks and slides? Amy, if you are a clinical lab and not research, CAP requires 10 years on blocks, slides and now positive control slides, two years on QC records, log books and requisitions. JTT ----- Original Message ----- From: "Amy Lee" To: "histonet" Sent: Thursday, August 02, 2007 2:27 PM Subject: [Histonet] How many years you keep paraffin blocks and slides? > Hello histonetters, > > Due to the limited space of my lab I need to discard some blocks and > slides. I am thinking setting a "rule" about how long I can keep paraffin > blocks and slides. I need to know how other people do it. Could you share > your "rules"? > > Thanks in advance, > Amy > > > --------------------------------- > Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's > on, when. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Aug 3 01:34:29 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Aug 3 01:34:35 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EAA7@wahtntex2.waht.swest.nhs.uk> 30 years in the UK but we tend to keep forever. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Real integrity is doing the right thing, knowing that nobody's going to know whether you did it or not. --Oprah Winfrey his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From lpwenk <@t> sbcglobal.net Fri Aug 3 04:59:19 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Aug 3 04:59:29 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual Message-ID: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> Need some help interpreting a CAP check list question. ANP.06440 Does the laboratory have a system documenting that all personnel are knowledgeable about the contents of procedure manuals relevant to the scope of their testing activities? I have been told as several NSH workshops, and also talking with various histotechs who have been inspected by CAP, that techs have to sign off on each procedure. That having one sheet in the front of the staining manual that says "I know and understand and will follow all the procedures in this manual" is not acceptable. However, there is no comment either way after the CAP checklist question. I've looked up the two NCCLS regs, and can't find it there either (but I also fall asleep trying to read the NCCLS regs). REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; Approved Guideline-Second Edition. NCCLS document HS1-A2 2) NCCLS. Application of a Quality Management System Model for Laboratory Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 Can someone point me in the right direction, or have I been misinformed all these years? I know employees don't have to sign off every year, only the director. I know employees have to sign off on new or changed procedures. But what do you do with a new employee, who has to read every procedure? Is one sheet OK, or do should there be a list of all the procedures, and they sign off on each one and date it? What if I'm inspecting a lab, and see that they don't have any record of employees reading the procedures, or just have one sheet in the front? Thanks in advance for input. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From Helen.Ilsley <@t> uct.ac.za Fri Aug 3 05:53:21 2007 From: Helen.Ilsley <@t> uct.ac.za (Helen Ilsley) Date: Fri Aug 3 06:00:55 2007 Subject: [Histonet] RECA-1 paper Message-ID: Hi I am looking for a paper by: Duijvestein,A.M et al : Antibodies defining rat endothelial cells: RECA-1 a pan-endothelial cell specific monoclonal antibody. Lab Invest 66:459(1992) We are unable to get in at our library and I was hoping someone on the histonet might have access to it and could send me an electronic copy. It would really be appreciated. Many thanks, Helen Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6398/6590 021-448 5935 (fax) From rjbuesa <@t> yahoo.com Fri Aug 3 07:27:36 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 3 07:27:47 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual In-Reply-To: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> Message-ID: <794810.91604.qm@web61215.mail.yahoo.com> Peggy: We used to have a front page in the SOP with the signatures of all the histotechs acknowledging the contents of all and each protocol. During the years we were inspected many times by CAP and that procedure was always accepted. Ren? J. Lee & Peggy Wenk wrote: Need some help interpreting a CAP check list question. ANP.06440 Does the laboratory have a system documenting that all personnel are knowledgeable about the contents of procedure manuals relevant to the scope of their testing activities? I have been told as several NSH workshops, and also talking with various histotechs who have been inspected by CAP, that techs have to sign off on each procedure. That having one sheet in the front of the staining manual that says "I know and understand and will follow all the procedures in this manual" is not acceptable. However, there is no comment either way after the CAP checklist question. I've looked up the two NCCLS regs, and can't find it there either (but I also fall asleep trying to read the NCCLS regs). REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; Approved Guideline-Second Edition. NCCLS document HS1-A2 2) NCCLS. Application of a Quality Management System Model for Laboratory Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 Can someone point me in the right direction, or have I been misinformed all these years? I know employees don't have to sign off every year, only the director. I know employees have to sign off on new or changed procedures. But what do you do with a new employee, who has to read every procedure? Is one sheet OK, or do should there be a list of all the procedures, and they sign off on each one and date it? What if I'm inspecting a lab, and see that they don't have any record of employees reading the procedures, or just have one sheet in the front? Thanks in advance for input. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From rjbuesa <@t> yahoo.com Fri Aug 3 07:22:43 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 3 07:32:52 2007 Subject: [Histonet] acid alcohol with immunos In-Reply-To: <2b2c74398f67d648851ed09dd952d42e@mail2.lcpath.com> Message-ID: <57647.89226.qm@web61215.mail.yahoo.com> Jennifer: Acid alcohol can be used if you use DAB as the chromogen, but it is not necessary if you just use hematoxylin during 30 seconds and "blue" it afterwards. You will get enough nuclear staining and the procedure will be shortened. Ren? J. Jennifer Cresor wrote: Hello all, I have been told in the past to NOT use acid alcohol to differentiate the hematoxylin on immuno stains and only to use a progressive hematoxylin staining method. I do not remember the reason why. Do any of you have experiences and opinions on this subject? Is there a chance to harm immuno stains using acid alcohol and if so why? Thank you for your time. Jennifer Longview, Wa. jcresor@lcpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From aboris <@t> agh.org Fri Aug 3 07:53:49 2007 From: aboris <@t> agh.org (Anthony F. Boris) Date: Fri Aug 3 07:49:47 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual References: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> Message-ID: <5D942CF4AE61B24E8DDF0DE22F2827551FEA34@mse2.agh.org> Hi Peggy, Copied below is a question from the CAP website. Note in the last 2 lines of the "Note" paragraph where it talks about signatures. One signature is not sufficient on a main page, but if you list each procedure on one page, you can initial each procedure on that one page. It wants multiple signatures. The intent seems to make sure each document is reviewed, but how does allowing a main page where you can sign your name 50 times ensure that you have read the manual? We have gone to signing each individual procedure, we feel that this is what will be required in the future. Good Luck, Tony ANP.03776 Phase II N/A YES NO Is there documentation of at least annual review of all policies and procedures in the anatomic pathology section by the current laboratory director or designee? NOTE: The director must ensure that the collection of policies and procedures is complete, current, and has been thoroughly reviewed by a knowledgeable person. Technical approaches must be scientifically valid and clinically relevant. To minimize the burden on the laboratory and reviewer(s), it is suggested that a schedule be developed whereby roughly 1/12 of all procedures are reviewed monthly. Paper/electronic signature review must be at the level of each procedure, or as multiple signatures on a listing of named procedures. A single signature on a Title Page or Index of all procedures is not sufficient documentation that each procedure has been carefully reviewed. Signature or initials on each page of a procedure is not required. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lee & Peggy Wenk Sent: Fri 8/3/2007 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Techs Documenting Knowing Procedure Manual Need some help interpreting a CAP check list question. ANP.06440 Does the laboratory have a system documenting that all personnel are knowledgeable about the contents of procedure manuals relevant to the scope of their testing activities? I have been told as several NSH workshops, and also talking with various histotechs who have been inspected by CAP, that techs have to sign off on each procedure. That having one sheet in the front of the staining manual that says "I know and understand and will follow all the procedures in this manual" is not acceptable. However, there is no comment either way after the CAP checklist question. I've looked up the two NCCLS regs, and can't find it there either (but I also fall asleep trying to read the NCCLS regs). REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; Approved Guideline-Second Edition. NCCLS document HS1-A2 2) NCCLS. Application of a Quality Management System Model for Laboratory Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 Can someone point me in the right direction, or have I been misinformed all these years? I know employees don't have to sign off every year, only the director. I know employees have to sign off on new or changed procedures. But what do you do with a new employee, who has to read every procedure? Is one sheet OK, or do should there be a list of all the procedures, and they sign off on each one and date it? What if I'm inspecting a lab, and see that they don't have any record of employees reading the procedures, or just have one sheet in the front? Thanks in advance for input. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Fri Aug 3 08:12:29 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Aug 3 08:12:36 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual In-Reply-To: <5D942CF4AE61B24E8DDF0DE22F2827551FEA34@mse2.agh.org> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE51A@EXCHANGEBE1.carle.com> Anthony, Your response is about lab director signing off each year but Peggy is asking about ANP.20150 and it has the comment "The form of this system is at the discretion of the Laboratory Director." I have a signoff page that lists all policies and procedures for each employee and keep it in their file. Each employee initials off on the reviewed item and I have never had a problem with any CAP inspector accepting that form in 25 years of inspections. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony F. Boris Sent: Friday, August 03, 2007 7:54 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Techs Documenting Knowing Procedure Manual Hi Peggy, Copied below is a question from the CAP website. Note in the last 2 lines of the "Note" paragraph where it talks about signatures. One signature is not sufficient on a main page, but if you list each procedure on one page, you can initial each procedure on that one page. It wants multiple signatures. The intent seems to make sure each document is reviewed, but how does allowing a main page where you can sign your name 50 times ensure that you have read the manual? We have gone to signing each individual procedure, we feel that this is what will be required in the future. Good Luck, Tony ANP.03776 Phase II N/A YES NO Is there documentation of at least annual review of all policies and procedures in the anatomic pathology section by the current laboratory director or designee? NOTE: The director must ensure that the collection of policies and procedures is complete, current, and has been thoroughly reviewed by a knowledgeable person. Technical approaches must be scientifically valid and clinically relevant. To minimize the burden on the laboratory and reviewer(s), it is suggested that a schedule be developed whereby roughly 1/12 of all procedures are reviewed monthly. Paper/electronic signature review must be at the level of each procedure, or as multiple signatures on a listing of named procedures. A single signature on a Title Page or Index of all procedures is not sufficient documentation that each procedure has been carefully reviewed. Signature or initials on each page of a procedure is not required. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lee & Peggy Wenk Sent: Fri 8/3/2007 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Techs Documenting Knowing Procedure Manual Need some help interpreting a CAP check list question. ANP.06440 Does the laboratory have a system documenting that all personnel are knowledgeable about the contents of procedure manuals relevant to the scope of their testing activities? I have been told as several NSH workshops, and also talking with various histotechs who have been inspected by CAP, that techs have to sign off on each procedure. That having one sheet in the front of the staining manual that says "I know and understand and will follow all the procedures in this manual" is not acceptable. However, there is no comment either way after the CAP checklist question. I've looked up the two NCCLS regs, and can't find it there either (but I also fall asleep trying to read the NCCLS regs). REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; Approved Guideline-Second Edition. NCCLS document HS1-A2 2) NCCLS. Application of a Quality Management System Model for Laboratory Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 Can someone point me in the right direction, or have I been misinformed all these years? I know employees don't have to sign off every year, only the director. I know employees have to sign off on new or changed procedures. But what do you do with a new employee, who has to read every procedure? Is one sheet OK, or do should there be a list of all the procedures, and they sign off on each one and date it? What if I'm inspecting a lab, and see that they don't have any record of employees reading the procedures, or just have one sheet in the front? Thanks in advance for input. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Fri Aug 3 09:09:28 2007 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri Aug 3 09:10:20 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? Message-ID: Amy, We are in NY and we are required to keep slides and blocks for 20 years though we do have more than that currently stored. Vicki AMCH >>> Amy Lee 8/2/2007 3:27 PM >>> Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From jnocito <@t> satx.rr.com Fri Aug 3 09:13:18 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 3 09:14:38 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual References: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> Message-ID: <006e01c7d5d8$710d0780$d49eae18@yourxhtr8hvc4p> Let me stir the pot (as only I can). Some people read too much into questions and therefore create more work than necessary. All that is required is that the techs review the procedures. Like Rene, I have one sheet in front of every manual that they sign off on. During my CAP inspection this year, one inspector insisted that I create a procedure for every specimen I gross. I told her (and showed her) tat I had 4 grossing manuals to go by. She insisted that I make policies. I told her that wasn't going to happen. She told me she had to do that. Point of the story: a signature page in front of the manuals is sufficient, don't create more work than you need to. JTT ----- Original Message ----- From: "Lee & Peggy Wenk" To: Sent: Friday, August 03, 2007 4:59 AM Subject: [Histonet] Techs Documenting Knowing Procedure Manual > Need some help interpreting a CAP check list question. > > ANP.06440 Does the laboratory have a system documenting that all > personnel > are knowledgeable about the contents of procedure manuals relevant to the > scope of their testing activities? > > I have been told as several NSH workshops, and also talking with various > histotechs who have been inspected by CAP, that techs have to sign off on > each procedure. That having one sheet in the front of the staining manual > that says "I know and understand and will follow all the procedures in > this > manual" is not acceptable. > > However, there is no comment either way after the CAP checklist question. > I've looked up the two NCCLS regs, and can't find it there either (but I > also fall asleep trying to read the NCCLS regs). > REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; > Approved Guideline-Second Edition. NCCLS document HS1-A2 > 2) NCCLS. Application of a Quality Management System Model for Laboratory > Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 > > Can someone point me in the right direction, or have I been misinformed > all > these years? > > I know employees don't have to sign off every year, only the director. I > know employees have to sign off on new or changed procedures. > > But what do you do with a new employee, who has to read every procedure? > Is > one sheet OK, or do should there be a list of all the procedures, and they > sign off on each one and date it? > > What if I'm inspecting a lab, and see that they don't have any record of > employees reading the procedures, or just have one sheet in the front? > > Thanks in advance for input. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgarhart <@t> system1.net Fri Aug 3 09:37:04 2007 From: rgarhart <@t> system1.net (Robert Garhart) Date: Fri Aug 3 09:37:40 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: References: Message-ID: I am a recruiter with many clients in search of HT/HTL for their labs. Feel free to call or email to further discuss these potential positions if you are open to change and located in or near one of the following areas. Greater Philadelphia Greater NYC Greater Boston Greater Dallas CT NJ OH North and South Florida Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net From Jackie.O'Connor <@t> abbott.com Fri Aug 3 09:48:31 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Aug 3 09:52:45 2007 Subject: [Histonet] Can anyone hear me? Message-ID: I've sent a couple of queries out this week, but haven't seen them pop up in my own in-box. I'm reluctant to post my questions again for fear of redundancy. Can anyone out there hear me? I'm screaming for help. Jackie O' From Janci.Wellborn <@t> stlukes-stl.com Fri Aug 3 09:55:53 2007 From: Janci.Wellborn <@t> stlukes-stl.com (Wellborn, Janci R) Date: Fri Aug 3 09:56:06 2007 Subject: [Histonet] Linistain Stainer In-Reply-To: Message-ID: <42DC3293408E094499C68DDDD45CA08E35C8B4@W3CEXCHANGE2.slh.stlukes.com> Hello, Does anyone use the Linistaine Random Access Stainer for their frozen section staining? If so, would you be willing to share how you have the staining row set-up for H & E staining? Janci R Wellborn, BS, BSeD, HTL (ASCP) Anatomic Pathology Supervisor St. Luke's Hospital 232 South Woods Mill Road Chesterfield, Missouri 63017 (314) 205-6228 janci.wellborn@stlukes-stl.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vicki Gauch Sent: Friday, August 03, 2007 9:09 AM To: histonet; Amy Lee Subject: Re: [Histonet] How many years you keep paraffin blocks andslides? Amy, We are in NY and we are required to keep slides and blocks for 20 years though we do have more than that currently stored. Vicki AMCH >>> Amy Lee 8/2/2007 3:27 PM >>> Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of St. Luke's Hospital. From nmeres <@t> gmu.edu Fri Aug 3 09:56:17 2007 From: nmeres <@t> gmu.edu (Norman Meres) Date: Fri Aug 3 09:56:39 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: References: Message-ID: Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Fri Aug 3 10:03:25 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Aug 3 10:03:38 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide. . In-Reply-To: Message-ID: <5CB39BCA5724F349BCB748675C6CA1A214543531@SJMEMXMB02.stjude.sjcrh.local> There may be some looking for a good position to come by; so if you are not looking, all you have to do is hit the delete key. Thanks, Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide. . Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions if you are open to change and located in or near one of the > following areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Aug 3 11:12:43 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Aug 3 10:13:14 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: Message-ID: This has been discussed in the past. I don't mind it. It doesn't hurt to see what jobs are open. It is not that time consuming to hit delete. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab <@t> hyhc.com Fri Aug 3 10:14:18 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Aug 3 10:13:22 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide Message-ID: I feel diffently. I have been on this listserv for along time and feel that I look forward to seeing the jobs that are open out there (one never knows when something could trip your trigger!!) There are not that many messages sent out from Recruiters (maybe a few a month) so in my opinion, just hit delete if you don't want to read their messages. Dawn -----Original Message----- From: Norman Meres [mailto:nmeres@gmu.edu] Sent: Friday, August 03, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions if you are open to change and located in or near one of the > following areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From mpence <@t> grhs.net Fri Aug 3 10:20:18 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Aug 3 10:20:33 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6A2@IS-E2K3.grhs.net> Welcome to the Listserve, Norm. Those previous responses are what they call here "being flamed" You have to learn to take and receive with this group of people. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Fri Aug 3 10:30:15 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri Aug 3 10:30:22 2007 Subject: [Histonet] Job opening at Penn State University Message-ID: We have an opening for a tech to work in the histology lab part time and part time elsewhere in the lab. This would be a full time wage payroll (no benefits). Working with animals tissues/samples. For more information or if interested contact me rjr6@psu.edu Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University From nmeres <@t> gmu.edu Fri Aug 3 10:33:03 2007 From: nmeres <@t> gmu.edu (Norman Meres) Date: Fri Aug 3 10:33:15 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C6A2@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838CA1C6A2@IS-E2K3.grhs.net> Message-ID: <87FE476B-4A6F-4F7C-B91C-C9B18BED750E@gmu.edu> Thanks Mike. If that's being flamed on this listserve, then I am okay with it. I think everyone was pretty civil, and I got a perspective on things. Obviously, most people don't mind job postings, so I think I can get on with them. Thanks again, Norman On Aug 3, 2007, at 11:20 AM, Mike Pence wrote: > Welcome to the Listserve, Norm. Those previous responses are what > they > call here "being flamed" You have to learn to take and receive with > this > group of people. > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman > Meres > Sent: Friday, August 03, 2007 9:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide > > > Okay, I am new here, and not completely familiar with the customs of > this listserve. I can only speak for myself. I didn't sign up for > this listserve to receive emails such as this. I am here to read > about the experiences of others, to share what tiny bit of > histological knowledge I have, and to ask those with considerably > more expertise to share theirs. > I don't want to belabor this, but I would be in favor of not having > recruiters come on this listserve and spam us. > Thanks, > Norman > > On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > >> >> I am a recruiter with many clients in search of HT/HTL for their >> labs. > >> Feel free to call or email to further discuss these potential >> positions >> if you are open to change and located in or near one of the following >> areas. >> >> Greater Philadelphia >> Greater NYC >> Greater Boston >> Greater Dallas >> CT >> NJ >> OH >> North and South Florida >> >> >> >> Robert Garhart >> Executive Recruiter >> System 1 Search >> 678-342-9029 Office >> rgarhart@system1.net >> Website: www.system1.net >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jmahoney <@t> alegent.org Fri Aug 3 10:35:25 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Aug 3 10:35:44 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual In-Reply-To: <006e01c7d5d8$710d0780$d49eae18@yourxhtr8hvc4p> References: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> <006e01c7d5d8$710d0780$d49eae18@yourxhtr8hvc4p> Message-ID: <46B304EE0200001A00014A26@gwia.alegent.org> I agree with Joe. I have been an inspector and been inspected many times over the years. I think the process Joe proposes meets CAP. The Pathologist or his/her designee must sign each procedure annually. The only other thing I look for is that each new or revised procedure is signed off by each person using that procedure. So, new employees just need to sign the manual. Everyone must sign off on new or revised procedures. If people want to do more, that is their choice, but not required. Jan Mahoney Alegent Health Omaha >>> "Joe Nocito" 08/03/2007 9:13 AM >>> Let me stir the pot (as only I can). Some people read too much into questions and therefore create more work than necessary. All that is required is that the techs review the procedures. Like Rene, I have one sheet in front of every manual that they sign off on. During my CAP inspection this year, one inspector insisted that I create a procedure for every specimen I gross. I told her (and showed her) tat I had 4 grossing manuals to go by. She insisted that I make policies. I told her that wasn't going to happen. She told me she had to do that. Point of the story: a signature page in front of the manuals is sufficient, don't create more work than you need to. JTT ----- Original Message ----- From: "Lee & Peggy Wenk" To: Sent: Friday, August 03, 2007 4:59 AM Subject: [Histonet] Techs Documenting Knowing Procedure Manual > Need some help interpreting a CAP check list question. > > ANP.06440 Does the laboratory have a system documenting that all > personnel > are knowledgeable about the contents of procedure manuals relevant to the > scope of their testing activities? > > I have been told as several NSH workshops, and also talking with various > histotechs who have been inspected by CAP, that techs have to sign off on > each procedure. That having one sheet in the front of the staining manual > that says "I know and understand and will follow all the procedures in > this > manual" is not acceptable. > > However, there is no comment either way after the CAP checklist question. > I've looked up the two NCCLS regs, and can't find it there either (but I > also fall asleep trying to read the NCCLS regs). > REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; > Approved Guideline-Second Edition. NCCLS document HS1-A2 > 2) NCCLS. Application of a Quality Management System Model for Laboratory > Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 > > Can someone point me in the right direction, or have I been misinformed > all > these years? > > I know employees don't have to sign off every year, only the director. I > know employees have to sign off on new or changed procedures. > > But what do you do with a new employee, who has to read every procedure? > Is > one sheet OK, or do should there be a list of all the procedures, and they > sign off on each one and date it? > > What if I'm inspecting a lab, and see that they don't have any record of > employees reading the procedures, or just have one sheet in the front? > > Thanks in advance for input. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Aug 3 11:01:47 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Aug 3 11:02:24 2007 Subject: [Histonet] I'll try this again for those in Texas Message-ID: This is the 4th try to post this question - so here' goes - IF you are in South Texas, AND are looking for an OJT histology trainee, please contact me. One of my beautiful, intelligent daughters lives in Jourdanton, south of San Antonio and would love to learn to be a histotech. Thanks. Jackie O' From ander093 <@t> tc.umn.edu Fri Aug 3 11:07:21 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri Aug 3 11:07:31 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: References: Message-ID: Personally, I like knowing what is available out there so I don't mind those posts. You can merely delete if you don't want to read them. At 09:56 AM 8/3/2007, you wrote: >Okay, I am new here, and not completely familiar with the customs of >this listserve. I can only speak for myself. I didn't sign up for >this listserve to receive emails such as this. I am here to read >about the experiences of others, to share what tiny bit of >histological knowledge I have, and to ask those with considerably >more expertise to share theirs. >I don't want to belabor this, but I would be in favor of not having >recruiters come on this listserve and spam us. >Thanks, >Norman > >On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > >> >>I am a recruiter with many clients in search of HT/HTL for their labs. >>Feel free to call or email to further discuss these potential >>positions >>if you are open to change and located in or near one of the following >>areas. >> >>Greater Philadelphia >>Greater NYC >>Greater Boston >>Greater Dallas >>CT >>NJ >>OH >>North and South Florida >> >> >> >>Robert Garhart >>Executive Recruiter >>System 1 Search >>678-342-9029 Office >>rgarhart@system1.net >>Website: www.system1.net >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Fri Aug 3 11:09:10 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Aug 3 11:09:16 2007 Subject: [Histonet] Histology Supervisor Position Message-ID: <5CB39BCA5724F349BCB748675C6CA1A214543533@SJMEMXMB02.stjude.sjcrh.local> Good Morning Everyone We currently have a Histology Supervisor Position in one of our research labs. St Jude is a great place to work so if anyone is interested just let me know or you can go to the St Jude Children's Research Hospital website and apply. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From jnocito <@t> satx.rr.com Fri Aug 3 11:34:32 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 3 11:34:58 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual References: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> <006e01c7d5d8$710d0780$d49eae18@yourxhtr8hvc4p> <46B304EE0200001A00014A26@gwia.alegent.org> Message-ID: <006801c7d5ec$330badb0$d49eae18@yourxhtr8hvc4p> Jan made a good point that I forgot to mention. If a procedure was added or greatly edited, I used those procedures to do an in-service. Everyone signed the in-service and I killed two birds with one stone: had an in-service and everyone signed the new or revised procedure. JTT ----- Original Message ----- From: "Janice Mahoney" To: ; "Joe Nocito" ; Sent: Friday, August 03, 2007 10:35 AM Subject: Re: [Histonet] Techs Documenting Knowing Procedure Manual >I agree with Joe. I have been an inspector and been inspected many > times over the years. I think the process Joe proposes meets CAP. The > Pathologist or his/her designee must sign each procedure annually. The > only other thing I look for is that each new or revised procedure is > signed off by each person using that procedure. > So, new employees just need to sign the manual. Everyone must sign off > on new or revised procedures. If people want to do more, that is their > choice, but not required. > Jan Mahoney > Alegent Health > Omaha > >>>> "Joe Nocito" 08/03/2007 9:13 AM >>> > Let me stir the pot (as only I can). Some people read too much into > questions and therefore create more work than necessary. All that is > required is that the techs review the procedures. Like Rene, I have one > > sheet in front of every manual that they sign off on. During my CAP > inspection this year, one inspector insisted that I create a procedure > for > every specimen I gross. I told her (and showed her) tat I had 4 > grossing > manuals to go by. She insisted that I make policies. I told her that > wasn't > going to happen. She told me she had to do that. Point of the story: a > > signature page in front of the manuals is sufficient, don't create more > work > than you need to. > > JTT > ----- Original Message ----- > From: "Lee & Peggy Wenk" > To: > Sent: Friday, August 03, 2007 4:59 AM > Subject: [Histonet] Techs Documenting Knowing Procedure Manual > > >> Need some help interpreting a CAP check list question. >> >> ANP.06440 Does the laboratory have a system documenting that all >> personnel >> are knowledgeable about the contents of procedure manuals relevant to > the >> scope of their testing activities? >> >> I have been told as several NSH workshops, and also talking with > various >> histotechs who have been inspected by CAP, that techs have to sign > off on >> each procedure. That having one sheet in the front of the staining > manual >> that says "I know and understand and will follow all the procedures > in >> this >> manual" is not acceptable. >> >> However, there is no comment either way after the CAP checklist > question. >> I've looked up the two NCCLS regs, and can't find it there either > (but I >> also fall asleep trying to read the NCCLS regs). >> REFERENCES: 1) NCCLS. A Quality Management System Model for Health > Care; >> Approved Guideline-Second Edition. NCCLS document HS1-A2 >> 2) NCCLS. Application of a Quality Management System Model for > Laboratory >> Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 >> >> Can someone point me in the right direction, or have I been > misinformed >> all >> these years? >> >> I know employees don't have to sign off every year, only the > director. I >> know employees have to sign off on new or changed procedures. >> >> But what do you do with a new employee, who has to read every > procedure? >> Is >> one sheet OK, or do should there be a list of all the procedures, and > they >> sign off on each one and date it? >> >> What if I'm inspecting a lab, and see that they don't have any record > of >> employees reading the procedures, or just have one sheet in the > front? >> >> Thanks in advance for input. >> >> Peggy A. Wenk, HTL(ASCP)SLS >> William Beaumont Hospital >> Royal Oak, MI 48073 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cmiller <@t> physlab.com Fri Aug 3 11:37:36 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Aug 3 11:41:13 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide. . In-Reply-To: <5CB39BCA5724F349BCB748675C6CA1A214543531@SJMEMXMB02.stjude.sjcrh.local> References: <5CB39BCA5724F349BCB748675C6CA1A214543531@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <003801c7d5ec$9b1cc2e0$3402a8c0@plab.local> Well said Charlene. I like to see what is available. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry, Charlene Sent: Friday, August 03, 2007 10:03 AM To: Norman Meres; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT/HTL Job Opportunities Nationwide. . There may be some looking for a good position to come by; so if you are not looking, all you have to do is hit the delete key. Thanks, Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide. . Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions if you are open to change and located in or near one of the > following areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From slappycraw <@t> yahoo.com Fri Aug 3 11:49:45 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Aug 3 11:54:06 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: Message-ID: <224970.27609.qm@web53610.mail.re2.yahoo.com> With the shortage of good techs out there, and if you've ever had to hire anyone, every little bit helps. You never know where you might find the next great addition to your team. LuAnn Anderson wrote: Personally, I like knowing what is available out there so I don't mind those posts. You can merely delete if you don't want to read them. At 09:56 AM 8/3/2007, you wrote: >Okay, I am new here, and not completely familiar with the customs of >this listserve. I can only speak for myself. I didn't sign up for >this listserve to receive emails such as this. I am here to read >about the experiences of others, to share what tiny bit of >histological knowledge I have, and to ask those with considerably >more expertise to share theirs. >I don't want to belabor this, but I would be in favor of not having >recruiters come on this listserve and spam us. >Thanks, >Norman > >On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > >> >>I am a recruiter with many clients in search of HT/HTL for their labs. >>Feel free to call or email to further discuss these potential >>positions >>if you are open to change and located in or near one of the following >>areas. >> >>Greater Philadelphia >>Greater NYC >>Greater Boston >>Greater Dallas >>CT >>NJ >>OH >>North and South Florida >> >> >> >>Robert Garhart >>Executive Recruiter >>System 1 Search >>678-342-9029 Office >>rgarhart@system1.net >>Website: www.system1.net >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From JMacDonald <@t> mtsac.edu Fri Aug 3 11:58:20 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Aug 3 11:58:29 2007 Subject: [Histonet] positions available Message-ID: 2 Opportunities for Histologic Techs!!! TECHNICAL SPECIALIST Field based person, Must live on West Coast near major airport, lots of travel. Work with major manufacturer of histology equipment installing instruments, training customers, And working closely with sales reps doing demos and training. TECHNICAL PRODUCT SUPPORT SPECIALIST In house working with customers on the phone, helping them solve issues with instruments. Must be mechanically inclined, able to read service manual and troubleshoot over the phone. Highly visible position for a histotechnician, strong in histology and immunohistology. Interested parties please call Leslie McCarthy, NS Charney & Associates at 800-827-9753. Send resume to: lesliem@nscharney.com www.nscharney.com From JMacDonald <@t> mtsac.edu Fri Aug 3 12:01:25 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Aug 3 12:01:38 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? In-Reply-To: <708930.69276.qm@web38007.mail.mud.yahoo.com> Message-ID: At my previous place of employment, I researched the federal requirement, the state requirement and CAP. I made sure that we met or exceeded the maximum requirement for each. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Amy Lee Sent by: histonet-bounces@lists.utsouthwestern.edu 08/02/2007 12:27 PM To histonet cc Subject [Histonet] How many years you keep paraffin blocks and slides? Hello histonetters, Due to the limited space of my lab I need to discard some blocks and slides. I am thinking setting a "rule" about how long I can keep paraffin blocks and slides. I need to know how other people do it. Could you share your "rules"? Thanks in advance, Amy --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Aug 3 12:03:00 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Aug 3 12:03:13 2007 Subject: [Histonet] position available Message-ID: Laboratory Associate/Histotech Specialty Laboratories is one of the largest esoteric medical laboratories in the United States. Since 1975 the company has enjoyed solid growth through scientific innovation and superior service. Located in Valencia, California in a three-year old, custom-designed, 200,000 square-foot facility, now part of Quest Diagnostics. Within the Valencia facility is a wide variety of clinical laboratories, conducting a broad range of assays. Among our specialty areas are Molecular Biology, Cellular Immunology, Tissue Culture, Flow Cytometry, Molecular Genetics, Cytogenetics, Microbiology, and Chemistry. The site also hosts a Clinical Trials Department and a Research & Development Group. We are currently seeking a Lab Associate who will be working in our Anatomic Pathology department. As the successful candidate, you will: - Prepare slides and cut sections of paraffin blocks to be used in IHC and FISH. - Collect, label, handle and process specimens. Help prepare chemicals, reagents - and solutions - Perform IHC using various instruments (e.g., Dako, Ventana and Bond-Max). - Operate and maintain selected lab equipment. - File, archive and retrieve slides/blocks, reports, requisitions and other paperwork. - Stain/counterstain and coverslip slides for examination by pathologist. - Perform validation of new antibodies on different staining platforms. Preferred candidates will have: ? High school diploma or general education (GED) equivalent ? Minimum of one year of histology experience and/or training ? Specimen handling and computer experience ? Histologic Technician/Histotechnologist certification from ASCP ? Ability to follow detailed written and oral instructions ? Experience in antibody validation procedures and FISH technique Along with competitive salaries and a variety of options for work scheduling, regular full-time employees who work at least 32 hours per week are eligible for Health and Dental plans, a vision plan and a 401(k) retirement plan - all of which include company contributions. There are also disability plans available, flexible savings plans and college savings plans. The company sponsors, and subsidizes, almost two dozen vanpools which transport employees from all over the greater Los Angeles area. Specialty is an equal opportunity employer and considers qualified applicants for employment without regard to race, color, creed, religion, national origin, sex, sexual orientation, gender identity and expression, age, disability, or Vietnam era, or other eligible veteran status, or any other protected factor. For immediate consideration please apply through our website: http://specialtylabs.hodesiq.com/job_start.asp From jb481 <@t> columbia.edu Fri Aug 3 12:09:23 2007 From: jb481 <@t> columbia.edu (Joshua Berman) Date: Fri Aug 3 12:09:24 2007 Subject: [Histonet] A flurry of questions about gelatin embedding, freezing, and mounting for mouse brain floating sections. Message-ID: <001a01c7d5f1$0a863270$3926a8c0@JoshB> I am soliciting opinions/advice about floating section ICC in mouse brain. i know this is probably pretty basic stuff, but any help will be greatly appreciated. The brains are fixed in 4% para, then cryoprotected with 20%, then 30% sucrose. I am trying to embed in gelatin rather than TBS/OCT because of the added control it gives in orienting the brain, the opportunity to put a side marker in the gelatin around the slice, and also because it is sometimes helpful in keeping potential "float away" regions (e.g. cortex in more posterior portions, mammilary nucleus...) connected to the rest of the brain. 1) Any suggestions about the ideal gelatin percentage? Should I add sucrose to get the gelatin to adhere better to the brain--or will that make the sections sticky and hard to handle. Is post fixing the gelatin (after the brain is embedded) a good idea? Should it then be rinsed in PBS for a while to get rid of excess fix? 2) In our first try at this using 15% gelatin, I noticed the method of freezing that we used for TBS/OCT--freezing in a cryomold imobilized in a dry ice ethanol slurry/snow did not seem to work well. The tissue was full of holes. Is this coincidence or is it because the solidified gelatin block is a poor heat conductor? If that is the case, should I just drop the block into isopentane/dry ice, or even a dry ice ethanol bath (instead of snow/slurry). 3) I need hints about getting the floating section on the slide at the end of the experiment in a quicker, more efficient manner. Thus far we have found some Triton-X 100 in the PBS to be helpful. Is there an ideal concentration? Are other additives helpful? Should we be using something other then Fisher Superfrost Plus slides? Any special brush or droplet techniques compatible with multiple sections on a slide? Thanks in advance for any help with these questions! Joshua Berman Department of Psychiatry Columbia University From MVaughan4 <@t> ucok.edu Fri Aug 3 12:29:10 2007 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Fri Aug 3 12:29:40 2007 Subject: [Histonet] Keep Job Postings Coming In-Reply-To: Message-ID: Histonetters, I have students who graduate with a BS in Biology who have learned basic techniques of sectioning, staining, and sometimes IHC, and I am always grateful to pass on any job opportunities for them. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From jgutierrez <@t> precisionpath.us Fri Aug 3 12:28:23 2007 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Fri Aug 3 12:30:57 2007 Subject: [Histonet] I'll try this again for those in Texas In-Reply-To: References: Message-ID: <016DD2614C714CF7986966F2A764985F@precisionpath.lcl> The UTHSCSA has a histology school if she wants to go that route. Check also with Ameripath South Texas 210-477-5800. University Hospital has an opening but they only hire registered techs. Good luck! Juan C. Gutierrez Precision Pathology Services 210-646-0890 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, August 03, 2007 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'll try this again for those in Texas This is the 4th try to post this question - so here' goes - IF you are in South Texas, AND are looking for an OJT histology trainee, please contact me. One of my beautiful, intelligent daughters lives in Jourdanton, south of San Antonio and would love to learn to be a histotech. Thanks. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Aug 3 13:18:14 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Aug 3 13:19:29 2007 Subject: [Histonet] Re: How many years you keep paraffin blocks and slides? Message-ID: Far be it from me to encourage independent thinking, rather than blind compliance with regulations - but it seems to me that this issue needs looking at afresh. Assuming that the dismal specialty of surgical pathology continues in its present form at all, we're clearly going to need to retain paraffin blocks for a great deal longer than ten years, perhaps indefinitely. The profusion of new molecular methods means that we're going to be doing procedures as yet undreamed of on our patient's old paraffin blocks. I think that the demand for such services will increase in the future. Since liability for obstetrical problems extends to the child's majority, plus discovery (i.e., around twenty years) we're going to have to be able to produce placental blocks and slides for that long. Obviously the storage problems are formidable, since paraffin blocks require temperature controlled storage, and slides are extremely heavy. Meanwhile, as hospitals add ever more bureaucrats and paper pushers, the demand to relinquish floor space increases. I think that the academics, the CAP, and other interested parties need to be looking into this. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From vazquezr <@t> ohsu.edu Fri Aug 3 13:35:17 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Aug 3 13:35:51 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide Message-ID: Norman, That is how some Histo techs find new and exciting jobs elsewhere. Say someone wants to move to CA or Florida, and this recruiter would be of great help. Just my opinion. Robyn OHSU >>> "Norman Meres" 8/3/2007 7:56 AM >>> Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Aug 3 13:40:52 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Aug 3 13:40:59 2007 Subject: [Histonet] Freaky Friday Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From TJasper <@t> smdc.org Fri Aug 3 13:50:57 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Aug 3 13:51:15 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A5F9@SCREECH.ntcampus.smdc.org> Just my 2 cents worth here Norman. Maybe the term "spam" is subjective, I don't know, but I most certainly would not consider recruiters on this listserver as "spam". When one comes along that wants to enlarge a body part or make me rich because he's the chancellor to an overthrown African dictator then I'd get on board with the spam thing. Also keep in mind that we have a serious shortage of staff and that folks in the field are just beginning to realize the financial compensation that is long overdue. I also believe someone suggested hitting the delete button. This is a good suggestion as many "legit" topics on the Histonet do not apply to all subscribers. And who knows Norman, someday you may want to enlist the service of one of these recruiters, either to obtain staff or a new job yourself. Sincerely, Thomas Jasper HT (ASCP) BAS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Norman Meres Sent: Friday, August 03, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From jfish <@t> gladstone.ucsf.edu Fri Aug 3 13:54:42 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Aug 3 13:54:54 2007 Subject: [Histonet] Freaky Friday In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <000c01c7d5ff$c08c37a0$2e0d010a@JFISH> Maybe have it powder coated??? I'm not sure what that is but my dad and brothers talk about powder coating all of the time, so it must be the in thing to do! Happy Friday, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Fri Aug 3 14:03:44 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Aug 3 14:04:14 2007 Subject: [Histonet] Freaky Friday Message-ID: I would use the high-temp black paint on a grill. Then fire it up, throw some ribs on and tap a keg. Now this really doesn't solve your water bath problem. But with a belly full of pork and beer, who cares. TGIF >>> "Breeden, Sara" 8/3/2007 2:40 PM >>> The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Fri Aug 3 14:06:16 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Aug 3 14:13:14 2007 Subject: [Histonet] Re: How many years you keep paraffin blocks and slides? In-Reply-To: Message-ID: <385470.57913.qm@web53608.mail.re2.yahoo.com> "Meanwhile, as hospitals add ever more bureaucrats and paper pushers, the demand to relinquish floor space increases." I love this statement it is so true. I think we should have a centralized dome stadium where all laboratories store their blocks and slides. Then you dig tunnel lines from it to every lab in the country and when someone requests blocks or slides they just put them in the pneumatic tube and whoosh off they go. Or we could use the staduim for all the bureaucrats and then have our block and slide space back, oh and keep the tubes in case we need a bureaucrat or two. It's Friday and I have no other excuse. RSRICHMOND@aol.com wrote: Far be it from me to encourage independent thinking, rather than blind compliance with regulations - but it seems to me that this issue needs looking at afresh. Assuming that the dismal specialty of surgical pathology continues in its present form at all, we're clearly going to need to retain paraffin blocks for a great deal longer than ten years, perhaps indefinitely. The profusion of new molecular methods means that we're going to be doing procedures as yet undreamed of on our patient's old paraffin blocks. I think that the demand for such services will increase in the future. Since liability for obstetrical problems extends to the child's majority, plus discovery (i.e., around twenty years) we're going to have to be able to produce placental blocks and slides for that long. Obviously the storage problems are formidable, since paraffin blocks require temperature controlled storage, and slides are extremely heavy. Meanwhile, as hospitals add ever more bureaucrats and paper pushers, the demand to relinquish floor space increases. I think that the academics, the CAP, and other interested parties need to be looking into this. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From sally.norton <@t> seattlechildrens.org Fri Aug 3 14:13:09 2007 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Fri Aug 3 14:13:31 2007 Subject: [Histonet] Re: How many years you keep paraffin blocks and slides? In-Reply-To: References: Message-ID: As a children's hospital, we keep blocks and slides forever. Sally Norton CHRMC, Seattle, Wa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Friday, August 03, 2007 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: How many years you keep paraffin blocks and slides? Far be it from me to encourage independent thinking, rather than blind compliance with regulations - but it seems to me that this issue needs looking at afresh. Assuming that the dismal specialty of surgical pathology continues in its present form at all, we're clearly going to need to retain paraffin blocks for a great deal longer than ten years, perhaps indefinitely. The profusion of new molecular methods means that we're going to be doing procedures as yet undreamed of on our patient's old paraffin blocks. I think that the demand for such services will increase in the future. Since liability for obstetrical problems extends to the child's majority, plus discovery (i.e., around twenty years) we're going to have to be able to produce placental blocks and slides for that long. Obviously the storage problems are formidable, since paraffin blocks require temperature controlled storage, and slides are extremely heavy. Meanwhile, as hospitals add ever more bureaucrats and paper pushers, the demand to relinquish floor space increases. I think that the academics, the CAP, and other interested parties need to be looking into this. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From DennisH <@t> cookchildrens.org Fri Aug 3 14:19:02 2007 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Fri Aug 3 14:19:24 2007 Subject: [Histonet] Freaky Friday Message-ID: We had the same problem; nothing worked. We now put a piece of 8x11 black construction paper under our water baths. When it gets old, wet, or dirty, we simply replace it. Works great, and a package lasts forever. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org >>> "Jo Dee Fish" 8/3/2007 1:54 PM >>> Maybe have it powder coated??? I'm not sure what that is but my dad and brothers talk about powder coating all of the time, so it must be the in thing to do! Happy Friday, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From slappycraw <@t> yahoo.com Fri Aug 3 14:17:12 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Aug 3 14:21:01 2007 Subject: [Histonet] Freaky Friday In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <244107.11259.qm@web53602.mail.re2.yahoo.com> How about using some really thick Weigert's Iron Hematoxylin and a coat of waterproofer. "Breeden, Sara" wrote: The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From gcallis <@t> montana.edu Fri Aug 3 14:23:19 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Aug 3 14:23:26 2007 Subject: Re blacken waterbath surface Re: [Histonet] Freaky Friday In-Reply-To: References: Message-ID: <6.0.0.22.1.20070803131625.01b31880@gemini.msu.montana.edu> Sara, If the bath is metal, a metalsmith or someone with metal working capability could re-anodize the surface. Sculptors/artists often know how to do this too, the guys with big blow torches and welding masks. Otherwise the high temperature black paint may be the only alternative. Be sure to have a spotlessly clean surface and maybe even repolish with some fine grit paper before painting or the paint might start peeling off onto those precious sections. A whole new artifact problem. > >>> "Breeden, Sara" 8/3/2007 2:40 PM >>> >The Friday Hour of Fuming is being replaced by Freaky Question Day. >Is >there a way to "re-blacken" the interior surface of a tissue flotation >bath? I've thought about high-temp black spray paint (like for BBQs, >engine parts, etc.) and quickly decided it probably would have some >oddball reaction with the whole tissue-floating thing. Any ideas out >there? And, no, I haven't asked the manufacturer - I'm just having my >own ideas. And that's often a dangerous place to be! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From b-frederick <@t> northwestern.edu Fri Aug 3 14:27:27 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Aug 3 14:27:58 2007 Subject: [Histonet] Re: How many years you keep paraffin blocks and slides? In-Reply-To: Message-ID: <000201c7d604$57360600$d00f7ca5@lurie.northwestern.edu> After reading all these replies and being involved in research on old blocks, I agree. We are trying to implement an agreement that if a patient has consented to banking and research that the cases should be flagged and if those blocks are on the edge of a retaining period, we want them in our facility-again provided the patient has consented for us to have their tissue. We may only end up with one block for the initial study,but it won't last forever. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Friday, August 03, 2007 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: How many years you keep paraffin blocks and slides? Far be it from me to encourage independent thinking, rather than blind compliance with regulations - but it seems to me that this issue needs looking at afresh. Assuming that the dismal specialty of surgical pathology continues in its present form at all, we're clearly going to need to retain paraffin blocks for a great deal longer than ten years, perhaps indefinitely. The profusion of new molecular methods means that we're going to be doing procedures as yet undreamed of on our patient's old paraffin blocks. I think that the demand for such services will increase in the future. Since liability for obstetrical problems extends to the child's majority, plus discovery (i.e., around twenty years) we're going to have to be able to produce placental blocks and slides for that long. Obviously the storage problems are formidable, since paraffin blocks require temperature controlled storage, and slides are extremely heavy. Meanwhile, as hospitals add ever more bureaucrats and paper pushers, the demand to relinquish floor space increases. I think that the academics, the CAP, and other interested parties need to be looking into this. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmeres <@t> gmu.edu Fri Aug 3 14:34:40 2007 From: nmeres <@t> gmu.edu (Norman Meres) Date: Fri Aug 3 14:34:52 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: References: Message-ID: <28160337-D1EB-43B7-9116-3B7D529B3BAD@gmu.edu> Okay okay.....I got it. On Aug 3, 2007, at 2:35 PM, Robyn Vazquez wrote: > Norman, > > That is how some Histo techs find new and exciting jobs elsewhere. > Say someone wants to move to CA or Florida, and this recruiter > would be of great help. > Just my opinion. > > Robyn > OHSU > > >>> "Norman Meres" 8/3/2007 7:56 AM >>> > Okay, I am new here, and not completely familiar with the customs of > this listserve. I can only speak for myself. I didn't sign up for > this listserve to receive emails such as this. I am here to read > about the experiences of others, to share what tiny bit of > histological knowledge I have, and to ask those with considerably > more expertise to share theirs. > I don't want to belabor this, but I would be in favor of not having > recruiters come on this listserve and spam us. > Thanks, > Norman > > On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > > > > I am a recruiter with many clients in search of HT/HTL for their > labs. > > Feel free to call or email to further discuss these potential > > positions > > if you are open to change and located in or near one of the > following > > areas. > > > > Greater Philadelphia > > Greater NYC > > Greater Boston > > Greater Dallas > > CT > > NJ > > OH > > North and South Florida > > > > > > > > Robert Garhart > > Executive Recruiter > > System 1 Search > > 678-342-9029 Office > > rgarhart@system1.net > > Website: www.system1.net > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nmeres <@t> gmu.edu Fri Aug 3 14:38:24 2007 From: nmeres <@t> gmu.edu (Norman Meres) Date: Fri Aug 3 14:42:03 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A5F9@SCREECH.ntcampus.smdc.org> References: <1A9F2A6C5762524799A816F1F09744CF0143A5F9@SCREECH.ntcampus.smdc.org> Message-ID: Okay......I get it....the consensus is that recruiters on the listserve is a good thing. Thank you all for your input....now can anyone answer my question about lobster epidermis and optimum freezing temp for cryotomy? On Aug 3, 2007, at 2:50 PM, Jasper, Thomas G. wrote: > Just my 2 cents worth here Norman. Maybe the term "spam" is > subjective, I don't know, but I most certainly would not consider > recruiters on this listserver as "spam". When one comes along that > wants to enlarge a body part or make me rich because he's the > chancellor to an overthrown African dictator then I'd get on board > with the spam thing. > Also keep in mind that we have a serious shortage of staff and that > folks in the field are just beginning to realize the financial > compensation that is long overdue. I also believe someone > suggested hitting the delete button. This is a good suggestion as > many "legit" topics on the Histonet do not apply to all > subscribers. And who knows Norman, someday you may want to enlist > the service of one of these recruiters, either to obtain staff or a > new job yourself. > > Sincerely, > > Thomas Jasper HT (ASCP) BAS > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Norman > Meres > Sent: Friday, August 03, 2007 9:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide > > > Okay, I am new here, and not completely familiar with the customs of > this listserve. I can only speak for myself. I didn't sign up for > this listserve to receive emails such as this. I am here to read > about the experiences of others, to share what tiny bit of > histological knowledge I have, and to ask those with considerably > more expertise to share theirs. > I don't want to belabor this, but I would be in favor of not having > recruiters come on this listserve and spam us. > Thanks, > Norman > > On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > >> >> I am a recruiter with many clients in search of HT/HTL for their >> labs. >> Feel free to call or email to further discuss these potential >> positions >> if you are open to change and located in or near one of the following >> areas. >> >> Greater Philadelphia >> Greater NYC >> Greater Boston >> Greater Dallas >> CT >> NJ >> OH >> North and South Florida >> >> >> >> Robert Garhart >> Executive Recruiter >> System 1 Search >> 678-342-9029 Office >> rgarhart@system1.net >> Website: www.system1.net >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain > confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, > dissemination, distribution or copying of it or its contents is > prohibited. As required by federal and state laws, you need to hold > this information as privileged and confidential. If you have > received this communication in error, please notify the sender and > destroy all copies of this communication and any attachments. > > From rjbuesa <@t> yahoo.com Fri Aug 3 15:24:17 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 3 15:31:10 2007 Subject: [Histonet] Freaky Friday In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <496042.76396.qm@web61222.mail.yahoo.com> Since the temperature in the water bath is not that high, we always (after cleaning thoroughly and scrapping any fleaking paint) used to paint it with FLAT black paint (regularly sonld at any hardware store). Let it dry during the weekend and use it on Monday. Ren? J. "Breeden, Sara" wrote: The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From denise.woodward <@t> uconn.edu Fri Aug 3 15:38:26 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri Aug 3 15:38:38 2007 Subject: [Histonet] Freaky Friday In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C401662EC@EXCHANGED.mgmt.ad.uconn.edu> Many moons ago, when I worked at a small hospital, the biomedical department was able to re-surface the inside of the flotation bath. When he brought it back it looked like new. I have no idea how he did it. Anyone out there have a biomed pro they can ask? 2 cents on a hot Friday, Denise Long Woodward UCONN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Fri Aug 3 15:40:54 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri Aug 3 15:41:03 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: References: Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C401662ED@EXCHANGED.mgmt.ad.uconn.edu> I'm waiting for that job listing for Tahiti to pop up one of these days! Can I claim first dibs now?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Aug 3 15:51:10 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Aug 3 15:55:18 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: <40AC6D73C2B95C4CA21B26B7BF380C401662ED@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: There's one in Hawaii - great hospital, too! Tahiti has too many mosquitoes. "Woodward, Denise" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2007 03:40 PM To "Norman Meres" , cc Subject RE: [Histonet] HT/HTL Job Opportunities Nationwide I'm waiting for that job listing for Tahiti to pop up one of these days! Can I claim first dibs now?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions > if you are open to change and located in or near one of the following > areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ecpenaflor <@t> ucsf.neuroimmunol.org Fri Aug 3 15:57:28 2007 From: ecpenaflor <@t> ucsf.neuroimmunol.org (Vin Penaflor) Date: Fri Aug 3 15:57:44 2007 Subject: [Histonet] Job Oppurtunities in the San Francisco/San Jose, CA Bay Area Message-ID: Hi, I am currently looking for a position involving histology, immunohistochemistry/immunofluorescence and/or confocal microscopy in the san Francisco Bay area? If anyone knows of any opportunities let me know. Thanks in advance! From bakevictoria <@t> gmail.com Fri Aug 3 15:54:39 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Aug 3 15:58:06 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: <40AC6D73C2B95C4CA21B26B7BF380C401662ED@EXCHANGED.mgmt.ad.uconn.edu> References: <40AC6D73C2B95C4CA21B26B7BF380C401662ED@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: <4f016b690708031354s129ad713md73644cacece6232@mail.gmail.com> I'll place dibs on the one for Alaska or New Foundland! On 8/3/07, Woodward, Denise wrote: > I'm waiting for that job listing for Tahiti to pop up one of these days! > > Can I claim first dibs now?? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman > Meres > Sent: Friday, August 03, 2007 10:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide > > Okay, I am new here, and not completely familiar with the customs of > this listserve. I can only speak for myself. I didn't sign up for > this listserve to receive emails such as this. I am here to read > about the experiences of others, to share what tiny bit of > histological knowledge I have, and to ask those with considerably > more expertise to share theirs. > I don't want to belabor this, but I would be in favor of not having > recruiters come on this listserve and spam us. > Thanks, > Norman > > On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > > > > I am a recruiter with many clients in search of HT/HTL for their labs. > > Feel free to call or email to further discuss these potential > > positions > > if you are open to change and located in or near one of the following > > areas. > > > > Greater Philadelphia > > Greater NYC > > Greater Boston > > Greater Dallas > > CT > > NJ > > OH > > North and South Florida > > > > > > > > Robert Garhart > > Executive Recruiter > > System 1 Search > > 678-342-9029 Office > > rgarhart@system1.net > > Website: www.system1.net > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AGrobe2555 <@t> aol.com Fri Aug 3 15:58:26 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Aug 3 16:00:00 2007 Subject: [Histonet] Re: Freaky friday/waterbath refinish Message-ID: I am only speculating, but has anyone tried Gunblack from the sporting goods store? This stuff is used on pistols/rifles to refinish/touch up/darken the metal. I'm not sure what the bath interior is made of, but this might do the trick. I think I have seen the blackening compound in a pen form. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From Jason.Wiese <@t> va.gov Fri Aug 3 16:02:36 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri Aug 3 16:02:47 2007 Subject: [Histonet] Re: Freaky friday/waterbath refinish In-Reply-To: References: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12B92@VHAV20MSGA3.v20.med.va.gov> I guess my last message didn't post (I forgot to hit reply to all), but here it is again just in case anyone wanted to see it... I would be willing to bet gun bluing would work. The stuff they use to "blue", which is actually pretty black, the barrel of a gun. They also make some touch up stuff with an applicator that would work just fine... AND... they make a spray on that you bake when done and it hardens like nails. By using it in a water bath that heats daily, it would only make the finish better... harder... My 2 cents... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Friday, August 03, 2007 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Freaky friday/waterbath refinish I am only speculating, but has anyone tried Gunblack from the sporting goods store? This stuff is used on pistols/rifles to refinish/touch up/darken the metal. I'm not sure what the bath interior is made of, but this might do the trick. I think I have seen the blackening compound in a pen form. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Aug 3 15:54:39 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Aug 3 16:04:49 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: <40AC6D73C2B95C4CA21B26B7BF380C401662ED@EXCHANGED.mgmt.ad.uconn.edu> References: <40AC6D73C2B95C4CA21B26B7BF380C401662ED@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: <4f016b690708031354s129ad713md73644cacece6232@mail.gmail.com> I'll place dibs on the one for Alaska or New Foundland! On 8/3/07, Woodward, Denise wrote: > I'm waiting for that job listing for Tahiti to pop up one of these days! > > Can I claim first dibs now?? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman > Meres > Sent: Friday, August 03, 2007 10:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide > > Okay, I am new here, and not completely familiar with the customs of > this listserve. I can only speak for myself. I didn't sign up for > this listserve to receive emails such as this. I am here to read > about the experiences of others, to share what tiny bit of > histological knowledge I have, and to ask those with considerably > more expertise to share theirs. > I don't want to belabor this, but I would be in favor of not having > recruiters come on this listserve and spam us. > Thanks, > Norman > > On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > > > > I am a recruiter with many clients in search of HT/HTL for their labs. > > Feel free to call or email to further discuss these potential > > positions > > if you are open to change and located in or near one of the following > > areas. > > > > Greater Philadelphia > > Greater NYC > > Greater Boston > > Greater Dallas > > CT > > NJ > > OH > > North and South Florida > > > > > > > > Robert Garhart > > Executive Recruiter > > System 1 Search > > 678-342-9029 Office > > rgarhart@system1.net > > Website: www.system1.net > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Charlene.Henry <@t> STJUDE.ORG Fri Aug 3 16:16:55 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Aug 3 16:20:26 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide. . In-Reply-To: Message-ID: <5CB39BCA5724F349BCB748675C6CA1A214543534@SJMEMXMB02.stjude.sjcrh.local> What is the pay scale in Hawaii and do they pay to move you????? If so that might be a pretty good idea. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, August 03, 2007 3:51 PM To: Woodward, Denise Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] HT/HTL Job Opportunities Nationwide. . There's one in Hawaii - great hospital, too! Tahiti has too many mosquitoes. "Woodward, Denise" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2007 03:40 PM To "Norman Meres" , cc Subject RE: [Histonet] HT/HTL Job Opportunities Nationwide I'm waiting for that job listing for Tahiti to pop up one of these days! Can I claim first dibs now?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions if you are open to change and located in or near one of the > following areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Fri Aug 3 17:16:39 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Aug 3 17:20:11 2007 Subject: [Histonet] Von Kossa Stain Message-ID: Hi all, Have you tried just the regular Von Kossa stain? The only problem I could see is the thinness of the sections might result in a not-very-dark stain. Does anyone have a Von Kossa protocol that works well with MMA embedded specimen? We are trying to stain for osteoblasts and any related calcium salts. Your help is much appreciated. Thanks. Jim Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190 _________________________________________________________________ [1]Learn.Laugh.Share. Reallivemoms is right place! References Visible links 1. http://g.msn.com/8HMBENUS/2740??PS=47575 Hidden links: 2. mailto:histonet@lists.utsouthwestern.edu From jnocito <@t> satx.rr.com Fri Aug 3 18:12:21 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 3 18:12:37 2007 Subject: [Histonet] Freaky Friday References: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> <40AC6D73C2B95C4CA21B26B7BF380C401662EC@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: <004201c7d623$c10c80d0$d49eae18@yourxhtr8hvc4p> I like Fred's idea the best, fire up the BBQ and tap a keg. OK gang, next Friday we all meet over Fred's place. JTT ----- Original Message ----- From: "Woodward, Denise" To: "Breeden, Sara" ; Sent: Friday, August 03, 2007 3:38 PM Subject: RE: [Histonet] Freaky Friday Many moons ago, when I worked at a small hospital, the biomedical department was able to re-surface the inside of the flotation bath. When he brought it back it looked like new. I have no idea how he did it. Anyone out there have a biomed pro they can ask? 2 cents on a hot Friday, Denise Long Woodward UCONN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Fri Aug 3 18:18:15 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Aug 3 18:22:42 2007 Subject: [Histonet] Techs Documenting Knowing Procedure Manual In-Reply-To: <006e01c7d5d8$710d0780$d49eae18@yourxhtr8hvc4p> References: <000101c7d5b4$f5ed55f0$0202a8c0@HPPav2> <006e01c7d5d8$710d0780$d49eae18@yourxhtr8hvc4p> Message-ID: Thanks Joe! Interesting how 'requirements' for inspections are sometimes created by 'well, I had to do that' by an inspector. It is good to get a consensus though, as usually a consensus reflects good to superior practices. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, August 03, 2007 7:13 AM To: lpwenk@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Techs Documenting Knowing Procedure Manual Let me stir the pot (as only I can). Some people read too much into questions and therefore create more work than necessary. All that is required is that the techs review the procedures. Like Rene, I have one sheet in front of every manual that they sign off on. During my CAP inspection this year, one inspector insisted that I create a procedure for every specimen I gross. I told her (and showed her) tat I had 4 grossing manuals to go by. She insisted that I make policies. I told her that wasn't going to happen. She told me she had to do that. Point of the story: a signature page in front of the manuals is sufficient, don't create more work than you need to. JTT ----- Original Message ----- From: "Lee & Peggy Wenk" To: Sent: Friday, August 03, 2007 4:59 AM Subject: [Histonet] Techs Documenting Knowing Procedure Manual > Need some help interpreting a CAP check list question. > > ANP.06440 Does the laboratory have a system documenting that all > personnel > are knowledgeable about the contents of procedure manuals relevant to the > scope of their testing activities? > > I have been told as several NSH workshops, and also talking with various > histotechs who have been inspected by CAP, that techs have to sign off on > each procedure. That having one sheet in the front of the staining manual > that says "I know and understand and will follow all the procedures in > this > manual" is not acceptable. > > However, there is no comment either way after the CAP checklist question. > I've looked up the two NCCLS regs, and can't find it there either (but I > also fall asleep trying to read the NCCLS regs). > REFERENCES: 1) NCCLS. A Quality Management System Model for Health Care; > Approved Guideline-Second Edition. NCCLS document HS1-A2 > 2) NCCLS. Application of a Quality Management System Model for Laboratory > Services; Approved Guideline-Third Edition. NCCLS document GP26-A3 > > Can someone point me in the right direction, or have I been misinformed > all > these years? > > I know employees don't have to sign off every year, only the director. I > know employees have to sign off on new or changed procedures. > > But what do you do with a new employee, who has to read every procedure? > Is > one sheet OK, or do should there be a list of all the procedures, and they > sign off on each one and date it? > > What if I'm inspecting a lab, and see that they don't have any record of > employees reading the procedures, or just have one sheet in the front? > > Thanks in advance for input. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Aug 3 19:25:37 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 3 19:29:14 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? (Amy Lee) Message-ID: <582736990708031725q4706964eva9004d6932fa8945@mail.gmail.com> Hi, We keep them forever. It is important to note they do not need to be kept on site. Any reasonably temperature controlled storage facility would be fine. (Unless of course there are some guidelines that say otherwise) I'd be hesitant to just pitch perfectly good tissue. There are so many research projects out there that would love to get a hold of this. As long as you de-identify the reports you could have quite a resource on your hands. Amos Brooks From amosbrooks <@t> gmail.com Fri Aug 3 19:25:37 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 3 19:39:05 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? (Amy Lee) Message-ID: <582736990708031725q4706964eva9004d6932fa8945@mail.gmail.com> Hi, We keep them forever. It is important to note they do not need to be kept on site. Any reasonably temperature controlled storage facility would be fine. (Unless of course there are some guidelines that say otherwise) I'd be hesitant to just pitch perfectly good tissue. There are so many research projects out there that would love to get a hold of this. As long as you de-identify the reports you could have quite a resource on your hands. Amos Brooks From amosbrooks <@t> gmail.com Fri Aug 3 19:25:37 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 3 19:59:07 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? (Amy Lee) Message-ID: <582736990708031725q4706964eva9004d6932fa8945@mail.gmail.com> Hi, We keep them forever. It is important to note they do not need to be kept on site. Any reasonably temperature controlled storage facility would be fine. (Unless of course there are some guidelines that say otherwise) I'd be hesitant to just pitch perfectly good tissue. There are so many research projects out there that would love to get a hold of this. As long as you de-identify the reports you could have quite a resource on your hands. Amos Brooks From amosbrooks <@t> gmail.com Fri Aug 3 19:25:37 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 3 20:24:54 2007 Subject: [Histonet] How many years you keep paraffin blocks and slides? (Amy Lee) Message-ID: <582736990708031725q4706964eva9004d6932fa8945@mail.gmail.com> Hi, We keep them forever. It is important to note they do not need to be kept on site. Any reasonably temperature controlled storage facility would be fine. (Unless of course there are some guidelines that say otherwise) I'd be hesitant to just pitch perfectly good tissue. There are so many research projects out there that would love to get a hold of this. As long as you de-identify the reports you could have quite a resource on your hands. Amos Brooks From sheila_adey <@t> hotmail.com Fri Aug 3 20:49:44 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Aug 3 20:49:58 2007 Subject: [Histonet] trichrome problems In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D970DC831@PHSXMB30.partners.org> Message-ID: We put our slides in an 74 degree oven for 10 minutes and then it gets the 12 min bake on the stainer going to water. This has improved our problems with the sections lifting off. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Sherwood, Margaret " >To: "Derek Papalegis" >, >Subject: RE: [Histonet] trichrome problems >Date: Thu, 2 Aug 2007 10:55:41 -0400 > >We routinely leave our slides in a 60 degree oven overnight before >staining. > >Peggy Sherwood >Lab Associate, Photopathology >Wellman Center for Photomedicine (W224) >Massachusetts General Hospital >55 Fruit Street >Boston, MA 02114-2696 >617-724-4839 (voice mail) >617-726-6983 (lab) >617-726-1206 (fax) >msherwood@partners.org > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Derek >Papalegis >Sent: Thursday, August 02, 2007 10:28 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] trichrome problems > > >Hi Everyone, >I am having trouble with the tissue staying on the slide when doing a >trichrome stain. I currently use the Richard Allen super up-rite slides. >I found that the tissue came off the slide after I put it in the heated >bouin's solution for an hour. I tried to leave it in room temp bouin's >overnight instead of using it heated and I found that some tissue has >stayed on but some has still fallen off. I have tried both leaving >slides out to dry overnight as well as cutting and staining on the same >day. Does anyone have any ideas of how I can solve this problem? > >Thanks, >Derek > >-- >Derek Papalegis HT (ASCP) >Histotechnician >Division of Laboratory Animal Medicine >Tufts University >136 Harrison Avenue >Boston, MA 02111 >phone: 617 636-2971 >fax: 617 636-8354 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >The information transmitted in this electronic communication is intended >only for the person or entity to whom it is addressed and may contain >confidential and/or privileged material. Any review, retransmission, >dissemination or other use of or taking of any action in reliance upon this >information by persons or entities other than the intended recipient is >prohibited. If you received this information in error, please contact the >Compliance HelpLine at 800-856-1983 and properly dispose of this >information. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Former Police Officer Paul Gillespie’s TAKE BACK THE INTERNET tips and tricks, watch the video now http://safety.sympatico.msn.ca/ From jkiernan <@t> uwo.ca Fri Aug 3 23:58:15 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Aug 3 23:58:27 2007 Subject: [Histonet] Can anyone hear me? In-Reply-To: References: Message-ID: Yes, I hear you (Sat 4th Augusst 2007). What is your question? It's not in the email tail (quoted below). John Kiernan London, Canada ----- Original Message ----- From: Jackie M O'Connor Date: Friday, August 3, 2007 10:53 Subject: [Histonet] Can anyone hear me? To: histonet@lists.utsouthwestern.edu > I've sent a couple of queries out this week, but haven't seen > them pop up > in my own in-box. I'm reluctant to post my questions again > for fear of > redundancy. > Can anyone out there hear me? I'm screaming for help. > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ccross6032 <@t> aol.com Sat Aug 4 07:58:42 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Sat Aug 4 07:59:57 2007 Subject: [Histonet] perfusion protocol for postnatal rats? Message-ID: <35FBA2DB-7814-4723-A781-0CC1B349C307@aol.com> hey everyone - does anyone have a perfusion protocol for postnatal rats (from P0 to P28 or so) that differers from their regular perfusion protocol? the tissues will be formalin fixed and paraffin embedded. Curious if people are using a different formalin %. Thanks! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From mickie25 <@t> netzero.net Sat Aug 4 09:26:51 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat Aug 4 09:30:32 2007 Subject: [Histonet] Freaky Friday In-Reply-To: <004201c7d623$c10c80d0$d49eae18@yourxhtr8hvc4p> References: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu><40AC6D73C2B95C4CA21B26B7BF380C401662EC@EXCHANGED.mgmt.ad.uconn.edu> <004201c7d623$c10c80d0$d49eae18@yourxhtr8hvc4p> Message-ID: Our Biomed department at Sacred Heart used to do this also. They used an Epoxy paint which held up for a few years. As the surface ages, bubbles are more of a problem as the surface gets pitted. I think they may also have baked it on a little, but not sure. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, August 03, 2007 4:12 PM To: Woodward, Denise; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Freaky Friday I like Fred's idea the best, fire up the BBQ and tap a keg. OK gang, next Friday we all meet over Fred's place. JTT ----- Original Message ----- From: "Woodward, Denise" To: "Breeden, Sara" ; Sent: Friday, August 03, 2007 3:38 PM Subject: RE: [Histonet] Freaky Friday Many moons ago, when I worked at a small hospital, the biomedical department was able to re-surface the inside of the flotation bath. When he brought it back it looked like new. I have no idea how he did it. Anyone out there have a biomed pro they can ask? 2 cents on a hot Friday, Denise Long Woodward UCONN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Aug 4 09:55:49 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Aug 4 10:03:31 2007 Subject: [Histonet] tape transfer sections Message-ID: <200708041455.l74EtoFx090303@pro12.abac.com> Would those working with TMA's prepared using the tape transfer technique please tell me again how you deal with the coating on the slides, I am having trouble with that interfering with the IHC staining. I have been soaking the slides in xylene for a couple of days before staining but still get hazy results. How are you mounting these slides after IHC? Do you use the cure mount reagent from Instrumedics? Thank you,, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net website www.ihctech.net From pruegg <@t> ihctech.net Sat Aug 4 10:20:07 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Aug 4 10:20:21 2007 Subject: [Histonet] Von Kossa Stain In-Reply-To: Message-ID: <200708041520.l74FK7wV099196@pro12.abac.com> I have done a lot of VK staining of GMA and MMA sections. It does not really stain for the osteoblasts but will reduce any calcified tissue and turn it black. It is a very simple stain, I use 2% silver nitrate in dih20 with very clean and dih20 rinsed glassware. Put the silver on the slides and place them under a uv light or even in a window where light is coming thru, this can take from 5-30 min to develop. Pour off the silver nitrate and rinse well with dih20. We use a special H&E counterstain which will stain the osteoblasts lining the calcified bone. As an added bonus the aqueous eosin we use will fluoresce. The osteoid (not yet calcified bone) can be seen under uv light as the eosin staining it will fluoresce. We use this method to measure osteoid thickness and the same slide on bright light to measure calcified bone thickness. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, August 03, 2007 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Von Kossa Stain Hi all, Have you tried just the regular Von Kossa stain? The only problem I could see is the thinness of the sections might result in a not-very-dark stain. Does anyone have a Von Kossa protocol that works well with MMA embedded specimen? We are trying to stain for osteoblasts and any related calcium salts. Your help is much appreciated. Thanks. Jim Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190 _________________________________________________________________ [1]Learn.Laugh.Share. Reallivemoms is right place! References Visible links 1. http://g.msn.com/8HMBENUS/2740??PS=47575 Hidden links: 2. mailto:histonet@lists.utsouthwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drkwolfe <@t> telus.net Sat Aug 4 10:43:25 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Sat Aug 4 10:43:38 2007 Subject: [Histonet] Keep Job Postings Coming In-Reply-To: Message-ID: I am a student currently working on my BSc for Histotechnology. I'm also working part time in a histology research lab. I got the position through word of mouth. And because the cost of education keeps rising, I'll be needing additional jobs just to pay for that schooling. It is also very encouraging to check my mail and see what the recruiters are offering this week. And from seeng some of my old childhood stomping grounds listed on the postings, I know that when the time comes, I can go back home where I grew up. My wife also being an American, can't wait for me to finish my degree and certification so that she can go back home. Rather than considering the recruiters an irritation, consider it as incentive for the next group of Histotechnologists, Pathologists, and even lab assistants. Seeing whats available out there will give a student a good boost to increase his grades, etc. So I agree, keep the recruitments coming Joe Kapler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of MVaughan4@ucok.edu Sent: Friday, August 03, 2007 11:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keep Job Postings Coming Histonetters, I have students who graduate with a BS in Biology who have learned basic techniques of sectioning, staining, and sometimes IHC, and I am always grateful to pass on any job opportunities for them. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.11.4/935 - Release Date: 8/3/2007 17:46 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.11.4/935 - Release Date: 8/3/2007 17:46 From Maxim_71 <@t> mail.ru Sat Aug 4 13:33:59 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Aug 4 13:37:15 2007 Subject: [Histonet] Re: Freaky friday/waterbath refinish Message-ID: <803693850.20070804223359@mail.ru> It is well known, that any steel things will become black, need heat it up to red, then dip in mineral oil and cool here. After such procedures this black colour possible to take away only long polish with abrasive. Best regards, Maxim Peshkov, Russia, Taganrog. From JWEEMS <@t> sjha.org Sat Aug 4 20:04:48 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Aug 4 20:09:02 2007 Subject: [Histonet] Freaky Friday In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F45D4@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA019@sjhaexc02.sjha.org> I always just used black spray paint - did it on Friday so it would dry well over the weekend. Make sure you scrape out any loose paint. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From talulahgosh <@t> gmail.com Sun Aug 5 02:41:38 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Aug 5 02:45:13 2007 Subject: [Histonet] Keep Job Postings Coming In-Reply-To: References: Message-ID: What does a BS in Histotechnology entail? I'm curious because I was hired out of college for my experience as a student intern and my degree in microbiology (nothing fancy--just doing maxipreps was the qualifier for my current job) That was eight years ago, and I'm wondering what other techniques I may not have learned while in college (again, let me emphasize that I went to college when the term pcr was not taught). I'm very interested in any techniques that are "high-tech", aka new within a couple of years. If you also know of a site, subscription, etc that would inform me of this, please let me know. I love learning new stuff about my job! I'd also like to add that I'm still paying off student loans after eight years--I feel your pain, Joe. I hope your loans aren't enormous and ps, you can always be a part-time student to defer your loans if absolutely necessary. On this note, is it necessary to have a college degree to be a histotechnologist (whatever you define this as)? At Pitt, you have to have a lot of experience to get paid about $30000 and that experience includes a BS. I've been around for eight years and have yet to break the $30000 barrier. Which makes me bitter. Our post-doc made about $40000 starting, if you need a scale. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. From amosbrooks <@t> gmail.com Sun Aug 5 08:35:43 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Aug 5 08:35:48 2007 Subject: [Histonet] Repeating Messages Message-ID: <582736990708050635q10d9ce3by3c876df032cfc9b8@mail.gmail.com> Hi, I apologize for the repeating messages. I know I only sent it once. I've seen a few messages by others do this as well. Does anyone know why this is happening? Is there a setting that I can change to prevent it from happening on my end, or is it just a server glitch? There is enough volume on this list without seeing multiple messages then to see myself doing it ... grrr! Regardless, I thank the List Admins for their tireless efforts on this useful & valuable tool. Thanks, Amos From mickie25 <@t> netzero.net Sun Aug 5 09:40:35 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sun Aug 5 09:45:14 2007 Subject: [Histonet] Re: Freaky friday/waterbath refinish In-Reply-To: <803693850.20070804223359@mail.ru> References: <803693850.20070804223359@mail.ru> Message-ID: HI All, Many waterbaths, like the old Technicon waterbaths and others have an aluminum pan so heating to make them black won't work. Oxidation of the aluminum causes the bottom to turn greenish grey. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Saturday, August 04, 2007 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Freaky friday/waterbath refinish It is well known, that any steel things will become black, need heat it up to red, then dip in mineral oil and cool here. After such procedures this black colour possible to take away only long polish with abrasive. Best regards, Maxim Peshkov, Russia, Taganrog. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Aug 5 14:42:59 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Aug 5 14:40:13 2007 Subject: [Histonet] Keep Job Postings Coming In-Reply-To: Message-ID: <200708051940.l75JdwEl034100@pro12.abac.com> Emily, The standard of practice for HT's is the ASCP certification. For HT you need only an associates of science degree with particular courses such as chemistry, biology and some math, then with OJT or a school of HT for one year. The HTL requires a BS degree also with certain course and the one year experience. Salaries have really gotten a lot better for Histotech's compared to my day (30 years ago), in my area experienced certified Histotech's are doing pretty well, because of the shortage of trained people in our field I see employers paying sign on bonuses, relocation expenses and decent salaries much like nurses are in demand today. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Sunday, August 05, 2007 1:42 AM To: Joseph Kapler; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Keep Job Postings Coming What does a BS in Histotechnology entail? I'm curious because I was hired out of college for my experience as a student intern and my degree in microbiology (nothing fancy--just doing maxipreps was the qualifier for my current job) That was eight years ago, and I'm wondering what other techniques I may not have learned while in college (again, let me emphasize that I went to college when the term pcr was not taught). I'm very interested in any techniques that are "high-tech", aka new within a couple of years. If you also know of a site, subscription, etc that would inform me of this, please let me know. I love learning new stuff about my job! I'd also like to add that I'm still paying off student loans after eight years--I feel your pain, Joe. I hope your loans aren't enormous and ps, you can always be a part-time student to defer your loans if absolutely necessary. On this note, is it necessary to have a college degree to be a histotechnologist (whatever you define this as)? At Pitt, you have to have a lot of experience to get paid about $30000 and that experience includes a BS. I've been around for eight years and have yet to break the $30000 barrier. Which makes me bitter. Our post-doc made about $40000 starting, if you need a scale. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmayo <@t> cscc.edu Sun Aug 5 15:05:40 2007 From: pmayo <@t> cscc.edu (Peggy Mayo) Date: Sun Aug 5 15:09:21 2007 Subject: [Histonet] Keep Job Postings Coming Message-ID: <46B5F5550200006700010678@gwgate.cscc.edu> To be more accurate, according to ASCP, there is 1 more route of eligibility toward HT certification. Follow this link to ASCP -- Board of Registry--website regarding this route: http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/eligibility/ht.aspx Peggy Mayo M.Ed. MLT (ASCP) Multi-Competency Health Technology 614-287-2608 or 800-621-6407 ext. 2608 Fax 614-287-3854 >>> "patsy ruegg" 08/05/07 3:42 PM >>> Emily, The standard of practice for HT's is the ASCP certification. For HT you need only an associates of science degree with particular courses such as chemistry, biology and some math, then with OJT or a school of HT for one year. The HTL requires a BS degree also with certain course and the one year experience. Salaries have really gotten a lot better for Histotech's compared to my day (30 years ago), in my area experienced certified Histotech's are doing pretty well, because of the shortage of trained people in our field I see employers paying sign on bonuses, relocation expenses and decent salaries much like nurses are in demand today. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Sunday, August 05, 2007 1:42 AM To: Joseph Kapler; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Keep Job Postings Coming What does a BS in Histotechnology entail? I'm curious because I was hired out of college for my experience as a student intern and my degree in microbiology (nothing fancy--just doing maxipreps was the qualifier for my current job) That was eight years ago, and I'm wondering what other techniques I may not have learned while in college (again, let me emphasize that I went to college when the term pcr was not taught). I'm very interested in any techniques that are "high-tech", aka new within a couple of years. If you also know of a site, subscription, etc that would inform me of this, please let me know. I love learning new stuff about my job! I'd also like to add that I'm still paying off student loans after eight years--I feel your pain, Joe. I hope your loans aren't enormous and ps, you can always be a part-time student to defer your loans if absolutely necessary. On this note, is it necessary to have a college degree to be a histotechnologist (whatever you define this as)? At Pitt, you have to have a lot of experience to get paid about $30000 and that experience includes a BS. I've been around for eight years and have yet to break the $30000 barrier. Which makes me bitter. Our post-doc made about $40000 starting, if you need a scale. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Aug 5 18:02:17 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 5 18:06:47 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide Message-ID: Would that be the lab at the Trippler(?spelling sorry) Medical Centre? I visited there about 25 years ago and was very impressed. Great Lab, Great People. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Saturday, 4 August 2007 6:51 AM To: Woodward, Denise Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] HT/HTL Job Opportunities Nationwide There's one in Hawaii - great hospital, too! Tahiti has too many mosquitoes. "Woodward, Denise" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2007 03:40 PM To "Norman Meres" , cc Subject RE: [Histonet] HT/HTL Job Opportunities Nationwide I'm waiting for that job listing for Tahiti to pop up one of these days! Can I claim first dibs now?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norman Meres Sent: Friday, August 03, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT/HTL Job Opportunities Nationwide Okay, I am new here, and not completely familiar with the customs of this listserve. I can only speak for myself. I didn't sign up for this listserve to receive emails such as this. I am here to read about the experiences of others, to share what tiny bit of histological knowledge I have, and to ask those with considerably more expertise to share theirs. I don't want to belabor this, but I would be in favor of not having recruiters come on this listserve and spam us. Thanks, Norman On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: > > I am a recruiter with many clients in search of HT/HTL for their labs. > Feel free to call or email to further discuss these potential > positions if you are open to change and located in or near one of the > following areas. > > Greater Philadelphia > Greater NYC > Greater Boston > Greater Dallas > CT > NJ > OH > North and South Florida > > > > Robert Garhart > Executive Recruiter > System 1 Search > 678-342-9029 Office > rgarhart@system1.net > Website: www.system1.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From drkwolfe <@t> telus.net Sun Aug 5 19:04:54 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Sun Aug 5 19:05:00 2007 Subject: [Histonet] Keep Job Postings Coming In-Reply-To: Message-ID: Emily In my particular case, I am taking my BSc with a Medical Lab Sciences major. I am also employed by a research lab, and therefore, I find myself given odd directions for study This week it could be zoology, next week its chemistry. Essentially, I am taking what courses I can through distance learning, all laboratory requirements are met through my employer, and the balance of the course work I take at the local university. Once I have finished my BSc, I will look to the American and Canadian Histotechnology Societies to determine if there are any additional courses I require for certification on both sides of the border. The ladies and gentlemen of Histonet can give a better description of the courses required for certification, as well, there are many links listed within the posts for some excellent sites related to Histotechnology, and the more advanced biochemical techniques. Yes, as a part-time student I would be able to defer some of my loans, which I will be doing, as I will be wanting to eventually get my PhD sometime before I retire. There are far too many branchs of study that can be followed once you get into the tree... time to shake the branches and see what falls out for me this week... -----Original Message----- From: Emily Sours [mailto:talulahgosh@gmail.com] Sent: Sunday, August 05, 2007 01:42 To: Joseph Kapler; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Keep Job Postings Coming What does a BS in Histotechnology entail? I'm curious because I was hired out of college for my experience as a student intern and my degree in microbiology (nothing fancy--just doing maxipreps was the qualifier for my current job) That was eight years ago, and I'm wondering what other techniques I may not have learned while in college (again, let me emphasize that I went to college when the term pcr was not taught). I'm very interested in any techniques that are "high-tech", aka new within a couple of years. If you also know of a site, subscription, etc that would inform me of this, please let me know. I love learning new stuff about my job! I'd also like to add that I'm still paying off student loans after eight years--I feel your pain, Joe. I hope your loans aren't enormous and ps, you can always be a part-time student to defer your loans if absolutely necessary. On this note, is it necessary to have a college degree to be a histotechnologist (whatever you define this as)? At Pitt, you have to have a lot of experience to get paid about $30000 and that experience includes a BS. I've been around for eight years and have yet to break the $30000 barrier. Which makes me bitter. Our post-doc made about $40000 starting, if you need a scale. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.11.6/938 - Release Date: 8/5/2007 16:16 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.11.6/938 - Release Date: 8/5/2007 16:16 From ree3 <@t> leicester.ac.uk Mon Aug 6 03:49:37 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Aug 6 03:49:49 2007 Subject: [Histonet] Re: Freaky friday/waterbath refinish In-Reply-To: References: <803693850.20070804223359@mail.ru> Message-ID: I just wear dark glasses, works a treat!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: 05 August 2007 15:41 To: 'Maxim Peshkov'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Freaky friday/waterbath refinish HI All, Many waterbaths, like the old Technicon waterbaths and others have an aluminum pan so heating to make them black won't work. Oxidation of the aluminum causes the bottom to turn greenish grey. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Saturday, August 04, 2007 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Freaky friday/waterbath refinish It is well known, that any steel things will become black, need heat it up to red, then dip in mineral oil and cool here. After such procedures this black colour possible to take away only long polish with abrasive. Best regards, Maxim Peshkov, Russia, Taganrog. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrcraigwbarlow <@t> googlemail.com Mon Aug 6 06:47:46 2007 From: mrcraigwbarlow <@t> googlemail.com (CRAIG BARLOW) Date: Mon Aug 6 06:47:53 2007 Subject: [Histonet] UK histology service Message-ID: <22482b560708060447j5a77c314t19e3fcdaba687ae5@mail.gmail.com> Hi Guy's, Would anybody be interested in out sourcing their histotechnology needs at the right price? All work carried out by time served CPA accredited Biomedical scientists and QC'd Fast turn around time Saves paying costly locums / extra staff To find out more please contect me. Regards Craig From sbreeden <@t> nmda.nmsu.edu Mon Aug 6 07:19:11 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Aug 6 07:22:07 2007 Subject: [Histonet] Freaky Friday Followup Flash Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F45EA@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to everyone that responded to my not-so-goofy question about resurfacing the inside of my round tissue float bath. I have Gleaned Much Information and will try the fine-sandpaper-and-flat-black-spray-paint method. Also, my husband just used some fine grit sandpaper to repair the two "dings" in my knife holder and it works just like new (that was last week's post from me). I think lots of us should get Frugality Awards for the $$ we save our employers, yeah? On the subject of block/slide saving, although this is a vet path lab, we have come to the agreement that we will save all slides for 10 years and blocks for 7 years. My logic for this is the life expectancy of animals vs humans and although this may be "back door" logic, it works for us (in my new building, which MAY be done right before I'm eligible to retire, the architect gave me an entire 11x22' room for slides and block storage with a locking, correctly ventilated volatile storage room!). I'm beside myself with anticipation!! At any rate, thank you to each of you that responded to my various questions - I do appreciate it very much! Well, it's Monday and the processor had a hiccup, so I'll be 3 hours behind all day. Sigh... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Aug 6 07:26:07 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Aug 6 07:26:15 2007 Subject: [Histonet] UK histology service Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EAD3@wahtntex2.waht.swest.nhs.uk> Is this a use of Histonet to procure contracts; is this acceptable behaviour for Vendors to procure customers? Does it contravene the 'rules'? Seen a few Americans attempt this and they were roundly flamed by all; odd that the e-mail carries a googlemail.com e-mail addy. Is that like Drain Cleaners and Tar Macadamers using a mobile phone number for anonymity? What's the name of the Company Craig? Do you have Professional Indemnity? Have you been CPA'd? Do you use full time Staff? Have you a phone number? Have you a presence on the Net? Are you a Solution to a Medical problem? Removed my phone number......... Kemlo Rogerson Pathology Manager Real integrity is doing the right thing, knowing that nobody's going to know whether you did it or not. --Oprah Winfrey his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kmb1904 <@t> aim.com Thu Aug 2 14:47:35 2007 From: kmb1904 <@t> aim.com (kmb1904@aim.com) Date: Mon Aug 6 08:33:59 2007 Subject: [Histonet] Entering tissue requests into Cerner. Message-ID: <8C9A3551BD5CE26-974-1167@FWM-D08.sysops.aol.com> Can anyone who uses Cerner tell me how they get the tissues ordered from the OR...GI ..etc??? We have always had the OR order them..but they also send a card for the pathos....do we really need to have them order them????? Or should we do it in Histology?? My concern is ...if we let them out of ordering..will it be on the patient record in Cerner??? Will we have to keep the paper requests forever??? What are the ramifications?? Can anyone give me a clue as to where to go with this??? Im in Delaware! Kathy Bowden bowdenk@nanticoke.org 302-629-6611 ext 2359 ________________________________________________________________________ Check Out the new free AIM(R) Mail -- Unlimited storage and industry-leading spam and email virus protection. From kmb1904 <@t> aim.com Fri Aug 3 04:20:27 2007 From: kmb1904 <@t> aim.com (kmb1904@aim.com) Date: Mon Aug 6 08:34:03 2007 Subject: [Histonet] Cerner orders from the OR... Message-ID: <8C9A3C6AA3D2150-9EC-50E6@webmail-md17.sysops.aol.com> Can anyone who uses Cerner tell me how they get the tissues ordered from the OR...GI ..etc??? We have always had the OR order them..but they also send a card for the pathos....do we really need to have them order them????? Or should we do it in Histology?? My concern is ...if we let them out of ordering..will it be on the patient record in Cerner??? Will we have to keep the paper requests forever??? What are the ramifications?? Can anyone give me a clue as to where to go with this??? Im in Delaware! Kathy Bowden bowdenk@nanticoke.org 302-629-6611 ext 2359 ________________________________________________________________________ Check Out the new free AIM(R) Mail -- Unlimited storage and industry-leading spam and email virus protection. From kmb1904 <@t> aim.com Sat Aug 4 06:57:18 2007 From: kmb1904 <@t> aim.com (kmb1904@aim.com) Date: Mon Aug 6 08:34:04 2007 Subject: [Histonet] (no subject) Message-ID: <8C9A4A5BDDAEFE1-C4-9F18@MBLK-M25.sysops.aol.com> I have been a member for years...but for some reason I cant post a question recently.? Do I need to join again?? And, to be honest..I dont know where to go to do that anylonger.? Can anyone help me? kmb1904@aol.com Thanks Kathy ________________________________________________________________________ Check Out the new free AIM(R) Mail -- Unlimited storage and industry-leading spam and email virus protection. From making <@t> ufl.edu Mon Aug 6 08:37:22 2007 From: making <@t> ufl.edu (MKing) Date: Mon Aug 6 08:47:40 2007 Subject: [Histonet] water bath black Message-ID: <46B72412.6090600@ufl.edu> We keep some large pieces of exposed xray film in the lab that are useful for creating black surfaces under work, usually for mounting sections but would probably also work to line the bottom of water baths. Washable/sterilizable, pretty inert. Mike King UF Pharmacology & Therapeutics --------------- Date: Fri, 3 Aug 2007 12:40:52 -0600 From: "Breeden, Sara" Subject: [Histonet] Freaky Friday To: Message-ID: ...Is there a way to "re-blacken" the interior surface of a tissue flotation bath? From akbitting <@t> geisinger.edu Mon Aug 6 08:42:35 2007 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Aug 6 08:54:50 2007 Subject: [Histonet] PMS-2 on Benchmarks? Message-ID: <46B6ED0B020000C90000D409@GHSGWIANW5V.GEISINGER.EDU> Anyone staining for PMS-2 on the Ventana Benchmarks: What dilution & protocol are you using? and where are you buying your antibody? Thanks for your help, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From making <@t> ufl.edu Mon Aug 6 08:44:08 2007 From: making <@t> ufl.edu (MKing) Date: Mon Aug 6 09:05:51 2007 Subject: [Histonet] gelatin embedding Message-ID: <46B725A8.2050405@ufl.edu> Joshua, This works pretty well, just be careful about potential interactions with immuno epitopes, especially near the tissue surface. Contact me if you have questions, Mike King UF Pharmacology & Therapeutics Gelatin-Albumin Embedding modified from Levin M. A novel immunohistochemical method for evaluation of antibody specificity and detection of labile targets in biological tissue. J Biochem Biophys Methods. 2004 Jan 30;58(1):85-96. 22.5 ml PBS 1.1 g gelatin 225 ml dH2O heat to 60 deg. C., dissolve completely cool to room temp., add 67.5 g egg albumin (bovine albumin ok), dissolve, aliquot, store frozen to use: thaw, add 420 ul formalin (37% formaldehyde) to 1.5 ml gelatin-albumin, mix thoroughly. pour into the bottom of molds over ice and allow to set (> 1 hr.). mix 2nd batch of formalin (1.5-3 ml for mouse brain) & gelatin-albumin, take specimen from 30% sucrose PBS, blot with Kimwipe, gently stir in formalin/gelatin-albumin, pour into mold and orient. allow to set, then spatula block out of mold, trim, equilibrate in 30% sucrose PBS, and section frozen. 210 ul glutaraldehyde can be used instead of formalin, but will react faster and may impair immunoreactivity more. ------------------ Date: Fri, 3 Aug 2007 13:09:23 -0400 From: "Joshua Berman" Subject: [Histonet] A flurry of questions about gelatin embedding, freezing, and mounting for mouse brain floating sections. To: Message-ID: <001a01c7d5f1$0a863270$3926a8c0@JoshB> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I am soliciting opinions/advice about floating section ICC in mouse brain. i know this is probably pretty basic stuff, but any help will be greatly appreciated... From ryan <@t> upei.ca Mon Aug 6 09:46:37 2007 From: ryan <@t> upei.ca (Dr. Catherine L. Ryan) Date: Mon Aug 6 09:14:05 2007 Subject: [Histonet] question about cresyl violet Message-ID: <46B6FC0D.16441.5664F1@localhost> I am having a problem with my cresyl violet stain. I am using cresyl violet acetate (1%) on 50 micron formalyn fixed rat brain cut with a vibratome. I am losing all stain at the final Histo-prep stage. In brief my procedure is as follows: I start with a series of alcohols (70- 95-100-95-70-50)- dH2O- cresyl violet-70%-95% EtOH - 95%+acetic acid to 95%-100% EtOH - Histo-prep wash. Any ideas or sugggestions for an improved protocol, would be appreciated. Cathy Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Aug 6 09:23:50 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Aug 6 09:49:42 2007 Subject: [Histonet] question about cresyl violet Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EADD@wahtntex2.waht.swest.nhs.uk> You are trying to demonstrate Nissl substance? Add 0.4 ml 10% acetic acid to 40 mls 0.1% cresyl echt violet in dist water, warm (35 to 40 degrees C). Stain sections 6 min, differentiate in 95% alcohol, dehydrate in two changes of absolute alcohol, clear in xylene and mount in synthetic resin. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Real integrity is doing the right thing, knowing that nobody's going to know whether you did it or not. --Oprah Winfrey his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Robinsoc <@t> mercyhealth.com Mon Aug 6 09:50:40 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Mon Aug 6 10:00:20 2007 Subject: [Histonet] Cerner orders from the OR... In-Reply-To: <8C9A3C6AA3D2150-9EC-50E6@webmail-md17.sysops.aol.com> References: <8C9A3C6AA3D2150-9EC-50E6@webmail-md17.sysops.aol.com> Message-ID: <46B6EEF0.59BC.00AF.0@mercyhealth.com> Kathy, We have Cerner and we do have nursing/physician place order in computer. All tissue specimens also have a card filled out with information for the card file. Do you have the total Cerner package, as in PowerChart, Pathnet, SurgNet, etc? If so, it depends on how your system is set up. Ours is set to interface results to PowerChart no matter where the order originated. If you want to discuss this further please contact me offline. Cindi Robinson, HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 >>> 8/3/2007 4:20 AM >>> Can anyone who uses Cerner tell me how they get the tissues ordered from the OR...GI ..etc??? We have always had the OR order them..but they also send a card for the pathos....do we really need to have them order them????? Or should we do it in Histology?? My concern is ...if we let them out of ordering..will it be on the patient record in Cerner??? Will we have to keep the paper requests forever??? What are the ramifications?? Can anyone give me a clue as to where to go with this??? Im in Delaware! Kathy Bowden bowdenk@nanticoke.org 302-629-6611 ext 2359 ________________________________________________________________________ Check Out the new free AIM(R) Mail -- Unlimited storage and industry-leading spam and email virus protection. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Mon Aug 6 10:01:59 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Mon Aug 6 10:10:40 2007 Subject: [Histonet] Pathos (microwave processing) advantages Message-ID: <9E6D52F532809247BDA1783680E92C560B702842@CHWEXC.chwi.chswi.org> I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From AFoshey <@t> chw.org Mon Aug 6 10:01:59 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Mon Aug 6 10:26:28 2007 Subject: [Histonet] Pathos (microwave processing) advantages Message-ID: <9E6D52F532809247BDA1783680E92C560B702842@CHWEXC.chwi.chswi.org> I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From ccrowder <@t> vetmed.lsu.edu Mon Aug 6 10:21:40 2007 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Mon Aug 6 10:26:51 2007 Subject: [Histonet] Urea and nitric acid Message-ID: Hi - For those of you who decalcify a lot. I have a graduate student who want to decalcify temporal bone from a dog using a protocol that uses 5% nitric acid and urea. The urea is supposed to clear the nitric acid color. Our question is - is there a specific urea what should be used. Having never used it myself, I have no idea what to buy. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From b-frederick <@t> northwestern.edu Mon Aug 6 10:27:59 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 6 10:28:14 2007 Subject: [Histonet] Freaky Friday In-Reply-To: Message-ID: <000001c7d83e$62661e20$d00f7ca5@lurie.northwestern.edu> Exactly what I do!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Friday, August 03, 2007 2:19 PM To: Jo Dee Fish; histonet@lists.utsouthwestern.edu; Sara' 'Breeden Subject: RE: [Histonet] Freaky Friday We had the same problem; nothing worked. We now put a piece of 8x11 black construction paper under our water baths. When it gets old, wet, or dirty, we simply replace it. Works great, and a package lasts forever. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org >>> "Jo Dee Fish" 8/3/2007 1:54 PM >>> Maybe have it powder coated??? I'm not sure what that is but my dad and brothers talk about powder coating all of the time, so it must be the in thing to do! Happy Friday, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, August 03, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freaky Friday The Friday Hour of Fuming is being replaced by Freaky Question Day. Is there a way to "re-blacken" the interior surface of a tissue flotation bath? I've thought about high-temp black spray paint (like for BBQs, engine parts, etc.) and quickly decided it probably would have some oddball reaction with the whole tissue-floating thing. Any ideas out there? And, no, I haven't asked the manufacturer - I'm just having my own ideas. And that's often a dangerous place to be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------------------------------------------------ ----------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Mon Aug 6 10:48:44 2007 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Aug 6 10:48:55 2007 Subject: [Histonet] HT/HTL Job Opportunities Nationwide In-Reply-To: References: Message-ID: <46B742DC.20806@umn.edu> I agree. It is important for us to know where the job opportunities are and what type of jobs. hit the delete key if the subject line is not of interest to you. Colleen Forster U of MN LuAnn Anderson wrote: > Personally, I like knowing what is available out there so I don't mind > those posts. You can merely delete if you don't want to read them. > > > At 09:56 AM 8/3/2007, you wrote: >> Okay, I am new here, and not completely familiar with the customs of >> this listserve. I can only speak for myself. I didn't sign up for >> this listserve to receive emails such as this. I am here to read >> about the experiences of others, to share what tiny bit of >> histological knowledge I have, and to ask those with considerably >> more expertise to share theirs. >> I don't want to belabor this, but I would be in favor of not having >> recruiters come on this listserve and spam us. >> Thanks, >> Norman >> >> On Aug 3, 2007, at 10:37 AM, Robert Garhart wrote: >> >>> >>> I am a recruiter with many clients in search of HT/HTL for their labs. >>> Feel free to call or email to further discuss these potential >>> positions >>> if you are open to change and located in or near one of the following >>> areas. >>> >>> Greater Philadelphia >>> Greater NYC >>> Greater Boston >>> Greater Dallas >>> CT >>> NJ >>> OH >>> North and South Florida >>> >>> >>> >>> Robert Garhart >>> Executive Recruiter >>> System 1 Search >>> 678-342-9029 Office >>> rgarhart@system1.net >>> Website: www.system1.net >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Mon Aug 6 10:52:21 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Aug 6 10:52:25 2007 Subject: [Histonet] Urea and nitric acid In-Reply-To: References: Message-ID: <6.0.0.22.1.20070806094417.01b344e8@gemini.msu.montana.edu> Hi Cheryl, I have never used urea in nitric acid, but simply 5% nitric acid, any higher concentration is a bit too concentrated. However, if the stock nitric acid has a yellow color to start with, DON'T use it. The yellow color means the acid is old and you should use new stock instead. Plus you may never get rid of the yellow discoloration. There is a nitric acid decalcifier caller Perenyi's (1882!) that gave us wonderful results years ago in a study, and is a bit gentler than 5% nitric acid. Nitric acid, 10% 40 ml Absolute ethanol 30 ml 0.5% chromic acid 30 ml We did decalcification endpoint determinations with this decalcifier, and had brilliant staining results. At 09:21 AM 8/6/2007, you wrote: >Hi - For those of you who decalcify a lot. I have a graduate student who >want to decalcify temporal bone from a dog using a protocol that uses 5% >nitric acid and urea. The urea is supposed to clear the nitric acid color. > Our question is - is there a specific urea what should be used. Having >never used it myself, I have no idea what to buy. >Cheryl Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From AFoshey <@t> chw.org Mon Aug 6 10:01:59 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Mon Aug 6 10:56:29 2007 Subject: [Histonet] Pathos (microwave processing) advantages Message-ID: <9E6D52F532809247BDA1783680E92C560B702842@CHWEXC.chwi.chswi.org> I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From rjbuesa <@t> yahoo.com Mon Aug 6 10:59:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 6 10:59:25 2007 Subject: [Histonet] Pathos (microwave processing) advantages In-Reply-To: <9E6D52F532809247BDA1783680E92C560B702842@CHWEXC.chwi.chswi.org> Message-ID: <184275.86718.qm@web61218.mail.yahoo.com> Annette: The fundamental advantage is that it is a "walk away" (automatic) tissue processor and that it can process up to 210 small biopsies in 1 hour, or in 4 hours if they are slices up to 5 mm thick. Low reagents consumption and the ability of using non proprietary solutions are also advantages. It costs around $ 119,000 (for sure "negotiable"). But the advantages stop there, because to have the 210 slides ready from a full load, you will still have to work an approximate total of 34.5 hours to complete your TAT with that number of samples processed. Ren? J. "Foshey, Annette" wrote: I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From doug <@t> ppspath.com Mon Aug 6 12:28:51 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Aug 6 11:31:13 2007 Subject: {SPAM?} Re: [Histonet] Pathos (microwave processing) advantages In-Reply-To: <184275.86718.qm@web61218.mail.yahoo.com> Message-ID: Rene, I think each facility has there own unique reason for looking into microwave processing. The majority want a faster TAT. We are using ours on a unique situation. We receive grossed tissue blocks around 10PM from a facility 2:30hr away. We have to get the samples processed and the slides have to be ready to go by 7AM. If we used the conventional processor our specimens would be under processed or we would be missing the deadline depending on the workload. This gives us a little more breathing room. We are still using the conventional processors on our routine biopsies. The non proprietary solutions are a big selling point. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, August 06, 2007 10:59 AM To: Foshey, Annette; histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] Pathos (microwave processing) advantages Annette: The fundamental advantage is that it is a "walk away" (automatic) tissue processor and that it can process up to 210 small biopsies in 1 hour, or in 4 hours if they are slices up to 5 mm thick. Low reagents consumption and the ability of using non proprietary solutions are also advantages. It costs around $ 119,000 (for sure "negotiable"). But the advantages stop there, because to have the 210 slides ready from a full load, you will still have to work an approximate total of 34.5 hours to complete your TAT with that number of samples processed. Ren? J. "Foshey, Annette" wrote: I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Mon Aug 6 12:02:41 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Aug 6 12:07:04 2007 Subject: [Histonet] Pathos (microwave processing) advantages In-Reply-To: <9E6D52F532809247BDA1783680E92C560B702842@CHWEXC.chwi.chswi.org> Message-ID: Annette, We have had the Pathos for about 10 months. We can do a surgical specimen run (breast and lipoma) in 5:05. No more reprocessing fatty specimens. You can also mix in biopsies without damaging the tissue. You can process non-fatty surgical specimens in less time of course. You can do rush cases quickly for same-day diagnosis. This works well for liver and other stat biopsies. You can use conventional formalin or a substitute if desired. It uses isopropyl alcohol as the clearing reagent. It is xylene free. It is fully automated so you can start a delayed run if needed. It is easy to maintain and chemical rotation and change is fully automated. It offers a wax cleaning step so you don't have to change wax that often, just top it off. The wax is in a separate retort so you do not have to wait for a cleaning cycle to begin to start another run. Anything else you would like to know feel free to ask. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Monday, August 06, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathos (microwave processing) advantages I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBranton <@t> sarapath.com Mon Aug 6 11:52:26 2007 From: BBranton <@t> sarapath.com (Brian Branton) Date: Mon Aug 6 12:07:06 2007 Subject: [Histonet] Histonet, Please unsubscribe me while I'm on vacation Message-ID: Hi Histonet, Please unsubscribe me while I'm on vacation, as I will be turning on the auto-reply rules on my email account. Thanks Brian Branton Purchasing Agent Sarasota Pathology (941) 362-8963 (941) 362-8964 FAX From AFoshey <@t> chw.org Mon Aug 6 10:01:59 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Mon Aug 6 12:07:08 2007 Subject: [Histonet] Pathos (microwave processing) advantages Message-ID: <9E6D52F532809247BDA1783680E92C560B702842@CHWEXC.chwi.chswi.org> I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From nuno_apct <@t> portugalmail.pt Mon Aug 6 12:00:33 2007 From: nuno_apct <@t> portugalmail.pt (nuno_apct@portugalmail.pt) Date: Mon Aug 6 12:07:14 2007 Subject: [Histonet] ASCP In-Reply-To: <20070806163554.9B6833E7FE9@eowyn.portugalmail.pt> References: <20070806163554.9B6833E7FE9@eowyn.portugalmail.pt> Message-ID: <1186419633.46b753b152d83@webmail4.portugalmail.pt> hello everybody!! I?m a portuguese HT and i would like to know what do i have to do to get a ascp certificate... i?m very interested in working on US but i don?t know how... please help :) Nuno Vasconcelos __________________________________________________________ Email gratuito com 2 000 MB Espa?o para guardar 20 anos de correio http://www.portugalmail.pt/2000mb From gu.lang <@t> gmx.at Mon Aug 6 13:18:04 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Aug 6 13:18:12 2007 Subject: [Histonet] fixation-question Message-ID: <000801c7d856$21f415a0$6412a8c0@dielangs.at> Hi to all fixation-experts, I've found in the literature some controversy statements about formaldehyd-fixation and want your help, what is the up-do-date-knowledge. - Formaldehyd can/cannot be washed out of fixed tissue. - Formaldehyd fixates slower in more acid pH; uncharched aminogroups react better than protonated with formaldehyd. And these groups should be uncharged in neutral pH. - Nuclear chromatin is fixed only by acid fixatives. What is your opinion on these statements? Regards Gudrun Lang From gu.lang <@t> gmx.at Mon Aug 6 13:00:10 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Aug 6 13:26:59 2007 Subject: AW: [Histonet] Pathos (microwave processing) advantages In-Reply-To: <20070806171309.29158gmx1@mx036.gmx.net> Message-ID: <000701c7d853$a250d4c0$6412a8c0@dielangs.at> Douglas, what are your experiences with semi-quantitative IHC like ER and Her2neu? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Douglas D Deltour Gesendet: Montag, 06. August 2007 19:03 An: 'Foshey, Annette'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Pathos (microwave processing) advantages Annette, We have had the Pathos for about 10 months. We can do a surgical specimen run (breast and lipoma) in 5:05. No more reprocessing fatty specimens. You can also mix in biopsies without damaging the tissue. You can process non-fatty surgical specimens in less time of course. You can do rush cases quickly for same-day diagnosis. This works well for liver and other stat biopsies. You can use conventional formalin or a substitute if desired. It uses isopropyl alcohol as the clearing reagent. It is xylene free. It is fully automated so you can start a delayed run if needed. It is easy to maintain and chemical rotation and change is fully automated. It offers a wax cleaning step so you don't have to change wax that often, just top it off. The wax is in a separate retort so you do not have to wait for a cleaning cycle to begin to start another run. Anything else you would like to know feel free to ask. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Monday, August 06, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathos (microwave processing) advantages I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wstover <@t> vet.uga.edu Mon Aug 6 13:49:12 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Mon Aug 6 13:49:18 2007 Subject: [Histonet] Histology Technician Opening Message-ID: The University of Georgia College of Veterinary Medicine has one histology technician job available. Start ASAP. Please call 706 583-0474 or visit the web at www.uga.edu. Great benefits and comparable salary. Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From MBURTON1 <@t> PARTNERS.ORG Mon Aug 6 13:58:01 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Mon Aug 6 13:53:12 2007 Subject: [Histonet] Pathos (microwave processing) advantages In-Reply-To: <65e975$52m47@phsmgmx10.partners.org> Message-ID: We are also interested in one of the Milestone microwave processors. A search of the Histonet archives returned mostly positive reviews. Can anyone add to this regarding the other Milestone models? Any major disadvantages or problems with RHS1? Thank you, Mark Burton HTL (ASCP) Brigham & Women's Hospital Boston, MA 02149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, August 06, 2007 1:03 PM To: 'Foshey, Annette'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathos (microwave processing) advantages Annette, We have had the Pathos for about 10 months. We can do a surgical specimen run (breast and lipoma) in 5:05. No more reprocessing fatty specimens. You can also mix in biopsies without damaging the tissue. You can process non-fatty surgical specimens in less time of course. You can do rush cases quickly for same-day diagnosis. This works well for liver and other stat biopsies. You can use conventional formalin or a substitute if desired. It uses isopropyl alcohol as the clearing reagent. It is xylene free. It is fully automated so you can start a delayed run if needed. It is easy to maintain and chemical rotation and change is fully automated. It offers a wax cleaning step so you don't have to change wax that often, just top it off. The wax is in a separate retort so you do not have to wait for a cleaning cycle to begin to start another run. Anything else you would like to know feel free to ask. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Monday, August 06, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathos (microwave processing) advantages I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From sjchtascp <@t> yahoo.com Mon Aug 6 13:58:49 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon Aug 6 13:58:53 2007 Subject: [Histonet] Skin PAS/D fungus Message-ID: <233380.84294.qm@web38203.mail.mud.yahoo.com> Working in a Derm Path Lab. Whats the least controls ones required to use doing a PAS/D fungus on skin? Thanks --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From doug <@t> ppspath.com Mon Aug 6 15:10:31 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Aug 6 14:11:49 2007 Subject: {SPAM?} [Histonet] Skin PAS/D fungus In-Reply-To: <233380.84294.qm@web38203.mail.mud.yahoo.com> Message-ID: ANP.21400 Phase II N/A YES NO Are positive controls run routinely on all special stains, with reactivity results documented, and are they verified for acceptability before reporting results? NOTE: A positive control slide must be run at the same time as any single or group of slides stained with the same special stain. The tissue chosen for the special stain control slide must be appropriate in type and amount. Both the control slide and the test tissue slide must be judged technically acceptable before the results of the special stains are reported. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Monday, August 06, 2007 1:59 PM To: Histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] Skin PAS/D fungus Working in a Derm Path Lab. Whats the least controls ones required to use doing a PAS/D fungus on skin? Thanks --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Aug 6 14:56:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 6 14:57:26 2007 Subject: [Histonet] Pathos (microwave processing) advantages In-Reply-To: Message-ID: <213828.23440.qm@web61224.mail.yahoo.com> Mark: From the economical point of view a small unit like the RHS-1 is a better option, although it has 2 drawbacks: HT exposure to chemicals when operating manually the MW oven, and the possibility of inconsistency in the processing since it has to be manually operated. On the other hand a small unit processing 15-20 cassettes every 2 hours (processing of 0.42 h + 0.53 h pre-processing tasks + 0.53 h of post-processing tasks). If each such a small run is processed by individual HTs, you will have a flow consisting of 15-20 slides ready every half an hour after an initial delay of 1 hour. The cost of the RHS-1 ( $38,000) is also a plus in the decision. Ren? J. "Burton, Mark" wrote: We are also interested in one of the Milestone microwave processors. A search of the Histonet archives returned mostly positive reviews. Can anyone add to this regarding the other Milestone models? Any major disadvantages or problems with RHS1? Thank you, Mark Burton HTL (ASCP) Brigham & Women's Hospital Boston, MA 02149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, August 06, 2007 1:03 PM To: 'Foshey, Annette'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathos (microwave processing) advantages Annette, We have had the Pathos for about 10 months. We can do a surgical specimen run (breast and lipoma) in 5:05. No more reprocessing fatty specimens. You can also mix in biopsies without damaging the tissue. You can process non-fatty surgical specimens in less time of course. You can do rush cases quickly for same-day diagnosis. This works well for liver and other stat biopsies. You can use conventional formalin or a substitute if desired. It uses isopropyl alcohol as the clearing reagent. It is xylene free. It is fully automated so you can start a delayed run if needed. It is easy to maintain and chemical rotation and change is fully automated. It offers a wax cleaning step so you don't have to change wax that often, just top it off. The wax is in a separate retort so you do not have to wait for a cleaning cycle to begin to start another run. Anything else you would like to know feel free to ask. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Monday, August 06, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathos (microwave processing) advantages I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From dusko.trajkovic <@t> pfizer.com Mon Aug 6 15:00:43 2007 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Mon Aug 6 15:00:57 2007 Subject: [Histonet] Please Unsubscribe Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B205074C45@lajamrexm01.amer.pfizer.com> ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From Sandra.Harrison3 <@t> va.gov Mon Aug 6 15:35:06 2007 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Aug 6 15:35:10 2007 Subject: [Histonet] Leica autostainer w/integrated coverslipper Message-ID: Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy From JMacDonald <@t> mtsac.edu Mon Aug 6 16:28:57 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Aug 6 16:29:06 2007 Subject: [Histonet] Histology Position in Northern California In-Reply-To: Message-ID: I am looking for a histotech familiar with preparing dermatology slides. Our lab is located in beautiful Sonoma County CA, the heart of the wine country and a hour from San Francisco. Mohs experience preferred but not necessary (we can train). Part to full time position available with benefits (medical, dental, 401K). Salary negotiable but will depend on experience and commitment. Fax resumes to 707-545-6726 Attn: Debi Thank you, Dr Sugarman From Barry.R.Rittman <@t> uth.tmc.edu Mon Aug 6 16:45:35 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Aug 6 16:45:46 2007 Subject: [Histonet] fixation-question In-Reply-To: <000801c7d856$21f415a0$6412a8c0@dielangs.at> Message-ID: Hi Short answer due to time constraints. See Pearse 1980. 1. Formalin can be removed from tissues as evidenced by the breaking of temporary bonds and reconstitution of tissue if fixation is relatively short. 2. No. In general formalin reacts more rapidly if in acidic pH. 3. Acidic fixatives such as Carnoy generally give much better fixation of nucleic acids than solutions such as formalin. With fixtives such as Bouin's the nucleic acids are precipitated rendering them more readily stained. Hope that this helps. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, August 06, 2007 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fixation-question Hi to all fixation-experts, I've found in the literature some controversy statements about formaldehyd-fixation and want your help, what is the up-do-date-knowledge. - Formaldehyd can/cannot be washed out of fixed tissue. - Formaldehyd fixates slower in more acid pH; uncharched aminogroups react better than protonated with formaldehyd. And these groups should be uncharged in neutral pH. - Nuclear chromatin is fixed only by acid fixatives. What is your opinion on these statements? Regards Gudrun Lang From laurie.colbert <@t> huntingtonhospital.com Mon Aug 6 16:53:44 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Aug 6 16:53:56 2007 Subject: [Histonet] Sakura Tissue Tek Coverslipper Message-ID: <57BE698966D5C54EAE8612E8941D768301268E6C@EXCHANGE3.huntingtonhospital.com> I am interested in hearing from anyone who has worked with the newer Sakura Tissue Tek Film Coverslipper - the one that has some type of carousel and will accommodate more than one rack at a time. I have the Sakura Prisma Stainer, and this coverslipper connects directly to the Prisma. Laurie Colbert From laurie.colbert <@t> huntingtonhospital.com Mon Aug 6 17:00:26 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Aug 6 17:00:31 2007 Subject: [Histonet] Leica Rep Message-ID: <57BE698966D5C54EAE8612E8941D768301268E6E@EXCHANGE3.huntingtonhospital.com> Would the Leica rep for the Pasadena, CA area please contact me. Laurie Colbert Huntington Memorial Hospital Pasadena, CA (626) 397-8620 From jkiernan <@t> uwo.ca Tue Aug 7 00:59:34 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Aug 7 01:00:22 2007 Subject: [Histonet] fixation-question In-Reply-To: <000801c7d856$21f415a0$6412a8c0@dielangs.at> References: <000801c7d856$21f415a0$6412a8c0@dielangs.at> Message-ID: Have you read and compared the statements you claim to have found "in the literature"? ----- Original Message ----- From: Gudrun Lang Date: Monday, August 6, 2007 14:19 Subject: [Histonet] fixation-question To: histonet@lists.utsouthwestern.edu > Hi to all fixation-experts, > > I've found in the literature some controversy statements about > formaldehyd-fixation and want your help, what is the up-do-date- > knowledge. > - > Formaldehyd can/cannot be washed out of fixed tissue. > > - > Formaldehyd fixates slower in more acid pH; uncharched > aminogroups react better than protonated with formaldehyd. And > these groups > should be uncharged in neutral pH. > > - Nuclear > chromatin is fixed only by acid fixatives. > > > > What is your opinion on these statements? > > > > Regards > > Gudrun Lang > > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Aug 7 01:52:13 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Aug 7 01:52:17 2007 Subject: [Histonet] fixation-question Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EAE3@wahtntex2.waht.swest.nhs.uk> Formalin can be 'washed out' but why would you want to fix in an acid pH? Do you like looking at pigment? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Real integrity is doing the right thing, knowing that nobody's going to know whether you did it or not. --Oprah Winfrey his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From garth <@t> apollosci.co.za Tue Aug 7 04:06:54 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Tue Aug 7 04:07:49 2007 Subject: [Histonet] Pathos (microwave processing) advantages In-Reply-To: <213828.23440.qm@web61224.mail.yahoo.com> Message-ID: <000e01c7d8d2$50da3d00$7700a8c0@jhb.apollosci.co.za> Hello everyone! I must agree with all the comments mentioned by all contributors. We have a PATHOS and a RHS-1, which we use regularly. I must further agree with Rene. In many cases, the RHS bench top models do offer better value. The key reason is that the RHS is multifunctional and, depending on your cassette / sample load, permits both rapid histo-processing and the ability to carry out value-added processes like epitope retrievals and decalcification.(Decalcification is very good, by the way!) The PATHOS is purely a histoprocessor. This means that if a laboratory requires a microwave for other processes, then an additional unit needs to be acquired. Having said this, a vendor will of course always try to market the bigger unit. The unit is fully automated and provides 'walk-away' operation. It is also a lot cheaper than other fully automated microwave processors on the market. (Ours cost less than R 850 000.00 at the time, which is about $ 110 000.00) Other comparable machines were in excess of R 1 million !) My advice is that in many cases, an RHS-1 or RHS-2 does present a better option for most histology laboratories. We are very happy with our RHS-1 and it has given excellent service! The cost was a big factor! Best regards Garth Jerome Histologist Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 06 August 2007 09:56 PM To: Burton, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathos (microwave processing) advantages Mark: From the economical point of view a small unit like the RHS-1 is a better option, although it has 2 drawbacks: HT exposure to chemicals when operating manually the MW oven, and the possibility of inconsistency in the processing since it has to be manually operated. On the other hand a small unit processing 15-20 cassettes every 2 hours (processing of 0.42 h + 0.53 h pre-processing tasks + 0.53 h of post-processing tasks). If each such a small run is processed by individual HTs, you will have a flow consisting of 15-20 slides ready every half an hour after an initial delay of 1 hour. The cost of the RHS-1 ( $38,000) is also a plus in the decision. Ren? J. "Burton, Mark" wrote: We are also interested in one of the Milestone microwave processors. A search of the Histonet archives returned mostly positive reviews. Can anyone add to this regarding the other Milestone models? Any major disadvantages or problems with RHS1? Thank you, Mark Burton HTL (ASCP) Brigham & Women's Hospital Boston, MA 02149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, August 06, 2007 1:03 PM To: 'Foshey, Annette'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathos (microwave processing) advantages Annette, We have had the Pathos for about 10 months. We can do a surgical specimen run (breast and lipoma) in 5:05. No more reprocessing fatty specimens. You can also mix in biopsies without damaging the tissue. You can process non-fatty surgical specimens in less time of course. You can do rush cases quickly for same-day diagnosis. This works well for liver and other stat biopsies. You can use conventional formalin or a substitute if desired. It uses isopropyl alcohol as the clearing reagent. It is xylene free. It is fully automated so you can start a delayed run if needed. It is easy to maintain and chemical rotation and change is fully automated. It offers a wax cleaning step so you don't have to change wax that often, just top it off. The wax is in a separate retort so you do not have to wait for a cleaning cycle to begin to start another run. Anything else you would like to know feel free to ask. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Monday, August 06, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathos (microwave processing) advantages I am interested in all the advantages the Pathos microwave processing has over conventional processing. If anyone is experienced with this please comment. Thank you. Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Tue Aug 7 08:17:33 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Aug 7 08:17:47 2007 Subject: [Histonet] Mouse tail processing Message-ID: <35e16a770708070617u38bc3fa5ncda7e3f38f942128@mail.gmail.com> Hello histonetters! I was wondering if anyone has ever processed mouse tails? Since there are all different types of tissue(cartilage, muscle, vessls, and skin), I would imagine that the processing times would be rather long. If someone is willing to share their protocolo, I would appreciate it greatly. Thank you. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA. From gu.lang <@t> gmx.at Tue Aug 7 08:31:40 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 7 08:33:31 2007 Subject: AW: [Histonet] fixation-question In-Reply-To: Message-ID: <001e01c7d8f7$4ad715c0$6412a8c0@dielangs.at> I have found the statements in Luna's "Histopathologic methods and color atlas of special stains and tissue artefacts" (1992) in the chapter "fixation". Formerly I have read your book, which impressed me very much. And after learning much about the staining-theory and pH, I thought Luna's statements were wrong or at least not understandable for me. What do you think? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _____ Von: John Kiernan [mailto:jkiernan@uwo.ca] Gesendet: Dienstag, 07. August 2007 08:00 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] fixation-question Have you read and compared the statements you claim to have found "in the literature"? ----- Original Message ----- From: Gudrun Lang Date: Monday, August 6, 2007 14:19 Subject: [Histonet] fixation-question To: histonet@lists.utsouthwestern.edu > Hi to all fixation-experts, > > I've found in the literature some controversy statements about > formaldehyd-fixation and want your help, what is the up-do-date- > knowledge. > - > Formaldehyd can/cannot be washed out of fixed tissue. > > - > Formaldehyd fixates slower in more acid pH; uncharched > aminogroups react better than protonated with formaldehyd. And > these groups > should be uncharged in neutral pH. > > - Nuclear > chromatin is fixed only by acid fixatives. > > > > What is your opinion on these statements? > > > > Regards > > Gudrun Lang > > From gu.lang <@t> gmx.at Tue Aug 7 08:46:37 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 7 08:46:54 2007 Subject: AW: [Histonet] Leica autostainer w/integrated coverslipper In-Reply-To: Message-ID: <002801c7d8f9$60485d40$6412a8c0@dielangs.at> Sandy, we have been using the leica stainer with adapted coverslipper for one year now. In the beginning there were some problems with the software. The stainer just stopped by accident until somebody recognized it and started it again. But these problems were solved and now it works like a clock. We have installed two parallel HE-protocols and some assistence-protocols for deparaffination and dehydration. Our stainer has the oven inside - so we can put in the paraffin-slides and get the ready-coverslipped slides out. The coverslipper is very reliable. We stain 200-300 slides per day within 3-4 hours with continuous loading. - depending on your protocol (ours is 65 min all incl.) For our purpose it's very good. The software could be faster. Hope this helps and regards Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Harrison, Sandra C. Gesendet: Montag, 06. August 2007 22:35 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Leica autostainer w/integrated coverslipper Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMINOKENUDSON <@t> PARTNERS.ORG Tue Aug 7 10:42:51 2007 From: MMINOKENUDSON <@t> PARTNERS.ORG (Mino-Kenudson, Mari,M.D.) Date: Tue Aug 7 10:43:04 2007 Subject: [Histonet] RE: NF kappa B Message-ID: Hello, We are trying to optimize 2 NF kappa B antibodies for immunohistochemistry on formalin-fixed, paraffin-embedded tissue. They are NFkB p50 (clone: sc-114) and NFkB p65 (clone: sc-8008) produced by Santa Cruz. We followed the instructions, but we only got very week signals. Any suggestions would be greatly appreciated. Thank you, Mari The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From liz <@t> premierlab.com Tue Aug 7 10:43:29 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Aug 7 10:43:43 2007 Subject: [Histonet] Mouse tail processing In-Reply-To: References: Message-ID: Igor We process tails like any other mouse joint such as paws, knees ankles, = we decal in formic acid (5-10%) and process on a long processing cycle. = Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor = Deyneko Sent: Tuesday, August 07, 2007 7:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse tail processing Hello histonetters! I was wondering if anyone has ever processed mouse tails? Since there = are all different types of tissue(cartilage, muscle, vessls, and skin), = I would imagine that the processing times would be rather long. If = someone is willing to share their protocolo, I would appreciate it = greatly. Thank you. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.11.8/940 - Release Date: 8/6/2007 = 4:53 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.11.8/940 - Release Date: 8/6/2007 = 4:53 PM =20 From karenadams <@t> comcast.net Tue Aug 7 10:50:26 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Tue Aug 7 10:50:40 2007 Subject: [Histonet] Supervisor question Message-ID: <080720071550.10645.46B894C2000396F00000299522070029539C030E0B0E020A9D0E05@comcast.net> Can anyone tell me if there is a requirement for a histology supervisor to be certified? if so, will ASCP eligibile suffice if the HTL exam will be taken w/in 3 years and the person has a BA degree.....thank you! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From rjbuesa <@t> yahoo.com Tue Aug 7 10:51:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 7 10:51:18 2007 Subject: [Histonet] RE: NF kappa B In-Reply-To: Message-ID: <677693.48472.qm@web61215.mail.yahoo.com> Sorry to say this but Santa Cruz antibodies were always very difficult to work with, so much so that I decided to stop using them altogether. Can you find another antibody source? Ren? J. "Mino-Kenudson, Mari,M.D." wrote: Hello, We are trying to optimize 2 NF kappa B antibodies for immunohistochemistry on formalin-fixed, paraffin-embedded tissue. They are NFkB p50 (clone: sc-114) and NFkB p65 (clone: sc-8008) produced by Santa Cruz. We followed the instructions, but we only got very week signals. Any suggestions would be greatly appreciated. Thank you, Mari The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From opiecurt <@t> yahoo.com Tue Aug 7 11:09:43 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Tue Aug 7 11:09:55 2007 Subject: [Histonet] please unsubscribe me Message-ID: <408546.3374.qm@web81611.mail.mud.yahoo.com> i've asked several times, am i doing this wrong or am i being ignored? LOL please unsubscribe me. --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From JWEEMS <@t> sjha.org Tue Aug 7 11:16:20 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Aug 7 11:16:42 2007 Subject: [Histonet] please unsubscribe me In-Reply-To: <408546.3374.qm@web81611.mail.mud.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA076@sjhaexc02.sjha.org> If you ever want to unsubscribe or change your options (eg, switch to or from digest mode, change your password, etc.), visit your subscription page at: http://lists.utsouthwestern.edu/mailman/options/histonet/jweems%40sjha.org Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of curt tague Sent: Tuesday, August 07, 2007 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] please unsubscribe me i've asked several times, am i doing this wrong or am i being ignored? LOL please unsubscribe me. --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From TJasper <@t> smdc.org Tue Aug 7 11:20:05 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Tue Aug 7 11:20:35 2007 Subject: [Histonet] Supervisor question In-Reply-To: <080720071550.10645.46B894C2000396F00000299522070029539C030E0B0E020A9D0E05@comcast.net> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A61A@SCREECH.ntcampus.smdc.org> Karen, To my knowledge the answer is determined by the employer. While I believe it is a good idea for supervisors to be certified I'm certain all are not. Depending on the individual (as well as the place of employment) it could work out all right or it might not. I think a non-certified supervisor should have a very good understanding of histology in order to be effective. Unfortunately, I've run into certified supervisors which weren't so hot, so at times the reverse must be true. Also, a certified tech with good skills doesn't always translate into a competent supervisor. Thomas Jasper HT (ASCP) BAS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of karenadams@comcast.net Sent: Tuesday, August 07, 2007 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Supervisor question Can anyone tell me if there is a requirement for a histology supervisor to be certified? if so, will ASCP eligibile suffice if the HTL exam will be taken w/in 3 years and the person has a BA degree.....thank you! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Heather.D.Renko <@t> osfhealthcare.org Tue Aug 7 11:25:19 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Aug 7 11:25:47 2007 Subject: [Histonet] Looking to buy a new autostainer Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775583@pmc-rfd-mx01.intranet.osfnet.org> I would also like to know of any thoughts with those working with the Leica XL stainer and the Sakura Autostainer. Thank you in advance. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From slappycraw <@t> yahoo.com Tue Aug 7 11:35:32 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Aug 7 11:35:44 2007 Subject: [Histonet] RE: NF kappa B In-Reply-To: Message-ID: <490797.60652.qm@web53612.mail.re2.yahoo.com> I use the NFkB p65 Santa Cruz (only one from them I do use) at 1:500 or .4ug/ml overnight and it has worked just fine. I recently tried the goat polymer from Biocare with the same antibody at 1:100,200,300,400, and 500 and still could go out a lot more. "Mino-Kenudson, Mari,M.D." wrote: Hello, We are trying to optimize 2 NF kappa B antibodies for immunohistochemistry on formalin-fixed, paraffin-embedded tissue. They are NFkB p50 (clone: sc-114) and NFkB p65 (clone: sc-8008) produced by Santa Cruz. We followed the instructions, but we only got very week signals. Any suggestions would be greatly appreciated. Thank you, Mari The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From Terry.Marshall <@t> rothgen.nhs.uk Tue Aug 7 11:29:07 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Aug 7 12:01:14 2007 Subject: [Histonet] please unsubscribe me Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6CCE@TRFT-EX01.xRothGen.nhs.uk> When all else fails - read the instructions. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of curt tague Sent: 07 August 2007 17:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] please unsubscribe me i've asked several times, am i doing this wrong or am i being ignored? LOL please unsubscribe me. --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Tue Aug 7 12:03:31 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Tue Aug 7 12:04:26 2007 Subject: [Histonet] Thank you! Message-ID: <374651.63520.qm@web38006.mail.mud.yahoo.com> Thank you so much to all who replied my email regarding the retention period of paraffin blocks and slides! I really appreciate your help! Amy --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From ryan <@t> upei.ca Tue Aug 7 13:29:32 2007 From: ryan <@t> upei.ca (Dr. Catherine L. Ryan) Date: Tue Aug 7 12:31:34 2007 Subject: [Histonet] camera lucida or drawing tube Message-ID: <46B881CA.3910.648D246@localhost> I have been looking for a camera lucida or drawing tube for a microscope. Any suggestions as to where I can purchase one (new or used) would be appreciated. Cathy Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA From bhewlett <@t> cogeco.ca Tue Aug 7 13:01:04 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Aug 7 13:01:18 2007 Subject: [Histonet] camera lucida or drawing tube References: <46B881CA.3910.648D246@localhost> Message-ID: <000701c7d91c$ec6ce660$6500a8c0@mainbox> For what make and model of microscope? Bryan ----- Original Message ----- From: "Dr. Catherine L. Ryan" To: Sent: Tuesday, August 07, 2007 2:29 PM Subject: [Histonet] camera lucida or drawing tube >I have been looking for a camera lucida or drawing tube for a > microscope. Any suggestions as to where I can purchase one (new > or used) would be appreciated. > Cathy > Dr. Catherine L. Ryan > Department of Psychology > University of Prince Edward Island > Charlottetown, P.E.I., Canada, C1A 4P3 > Ph# (902)566-0323/ FAX#(902)628-4359 > email: RYAN@UPEI.CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vsnider <@t> shrinenet.org Tue Aug 7 13:22:45 2007 From: vsnider <@t> shrinenet.org (Snider, Vivian Deanna) Date: Tue Aug 7 13:22:59 2007 Subject: [Histonet] PasD +/- controls Message-ID: <84BE46B37B314D409C5A17B7BAB022D601664B71@IDC-EX-VS01.shriners.cc> Steve, I have worked in facilities that required + and - with the stain and I have also worked in places that required only a positive control. I think it depends on the facility and their own set protocols. I am old school and still do + and - control. Deanna Snider HT ASCP Lead Histotechnician Shriners Hospital for Children Cincinnati, Oh 45239 513-872-6388 Message: 9 Date: Mon, 6 Aug 2007 11:58:49 -0700 (PDT) From: Steven Coakley Subject: [Histonet] Skin PAS/D fungus To: Histonet@lists.utsouthwestern.edu Message-ID: <233380.84294.qm@web38203.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Working in a Derm Path Lab. Whats the least controls ones required to use doing a PAS/D fungus on skin? Thanks CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From Erin.Martin <@t> ucsf.edu Tue Aug 7 13:54:18 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Aug 7 13:56:24 2007 Subject: [Histonet] Reference book? Message-ID: Hello all, Could anyone please recommend a good histo reference book to have on hand in the lab? The one I have is a 15 year old edition of Freida Carson's - I hope there has been something good that has come out in the last few years! Thanks, Erin Martin UCSF DermPath From rjbuesa <@t> yahoo.com Tue Aug 7 14:24:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 7 14:26:40 2007 Subject: [Histonet] camera lucida or drawing tube In-Reply-To: <46B881CA.3910.648D246@localhost> Message-ID: <517170.97301.qm@web61214.mail.yahoo.com> Not long ago I saw one in eBay. If it is placed instead of the ocular any one could be used in any microscope (ocular tubes of standard diameter according with the Royal Society).. Ren? J. "Dr. Catherine L. Ryan" wrote: I have been looking for a camera lucida or drawing tube for a microscope. Any suggestions as to where I can purchase one (new or used) would be appreciated. Cathy Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From rjbuesa <@t> yahoo.com Tue Aug 7 14:26:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 7 14:26:41 2007 Subject: [Histonet] Reference book? In-Reply-To: Message-ID: <461410.96582.qm@web61214.mail.yahoo.com> Your book will do. Remember that you probably are going to do standard HC procedures much of which are close to 100 years old! Ren? J. "Martin, Erin" wrote: Hello all, Could anyone please recommend a good histo reference book to have on hand in the lab? The one I have is a 15 year old edition of Freida Carson's - I hope there has been something good that has come out in the last few years! Thanks, Erin Martin UCSF DermPath _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From sccrshlly <@t> yahoo.com Tue Aug 7 16:27:51 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Tue Aug 7 16:28:03 2007 Subject: [Histonet] Pathos (microwave processing) advantages Message-ID: <459134.34048.qm@web90312.mail.mud.yahoo.com> We use the Milestone RHS II and love it. You do have to change the alcohols(ethanol and isopropyl) every 2 runs but we can run a biopsy run in less than an hour if the tissue is already fixed. There is no xylene, thus less waste and fumes. We only here complaints from the pathologist when either 1) the alcohols were not changed when necessary or 2) the program the user selected is not long enough for the tissue being processed. The only drawback is capacity...it only holds about 112 cassettes fully loaded. We only process biopsies, so the runs are so short, we can easily do two runs each day. The tissue looks great. Good luck! --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From marja <@t> biol.uni.torun.pl Tue Aug 7 18:23:22 2007 From: marja <@t> biol.uni.torun.pl (Marta Jaroszewska) Date: Tue Aug 7 18:24:01 2007 Subject: [Histonet] crysel violet stainning problem Message-ID: <002901c7d94a$04f2b250$1e236ba4@marta7e9f1c1cd> I'm writing because I have problem with de-staining of my samples during the staining with crysel violet. I read from the message from 2003 that the way is to dry samples in the oven, and don't use the water and alcoholes for dehydratation and differentiation. I tried to add acetic acid to alcoholes, to avoid 70, 95%...And nothing. The sections are a little bit blue after even 12 mins in crysel violet an than they are destained. I tried it with different samples, from formalin, Bouin's fixative. Finnaly, I have to try with drying in the oven but now I try to find other resolution. Crysel violet is from Sigma-Aldrich. I will appreciate for your help. Thanks From lpwenk <@t> sbcglobal.net Tue Aug 7 19:02:37 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Aug 7 19:02:53 2007 Subject: [Histonet] crysel violet stainning problem In-Reply-To: <002901c7d94a$04f2b250$1e236ba4@marta7e9f1c1cd> Message-ID: <001401c7d94f$6e5b27e0$0202a8c0@HPPav2> Is this the procedure for staining Nissl in brain? I don't know if cresyl violet is still around any more. I think it stopped being made around WWII. Most people are using cresyl echt violet, which now a-days is actually cresyl violet acetate. So, if you are using a bottle of cresyl violet, it's either very old, or it may be a different dye than what you need, being called that name. After all, any company can call any dye by any name. So the same name may be used for different dyes. Or the same dye many be called by different dyes. We found that we needed to buffer the solution to a pH of 3.5, to reduce background staining. Also, make certain you are using cresyl violet acetate certified by the Biological Stain Commission, if in the US. Assures good quality of dye. There is no Color Index number, as it is a mixture of dyes. Below is our staining procedure, which works for our lab and our students. If in a hurry, can stain in 60 degree C. waterbath or oven for about 20-30 minutes, or heat to 60-70 degrees C. in microwave and allow to set on counter for 5 minutes. CRESYL ECHT VIOLET 0.5 g Cresyl echt violet (Cresyl Violet Acetate) 0.18 g Sodium acetate (CH3COONaC3H2O) 500 ml Distilled water 1.5 mL Acetic acid, concentrated (CH3COOH) Dissolve cresyl echt violet and sodium acetate in distilled water. Slowly add acetic acid, drop by drop, to solution. Should have a pH of 3.5. If solution pH is below 3.5, add more sodium acetate. If solution pH is above 3.5, add more acetic acid. Filter. Let stand overnight before using. Store at room temperature. Stable for months. May be reused until weak. PROCEDURE - Cresyl Echt Violet: 1. Deparaffinize and hydrate slides through graded alcohol to distilled water. 2. Place sections in cresyl echt violet solution at room temperature 1-2 hours 3. Differentiate in two changes of 95% ethanol until nuclei and Nissl granules remain violet and the background is nearly colorless. Check differentiation with the microscope. 1 to 2 drops of acetic acid many be added to the first alcohol to speed up differentiation. 4. Dehydrate through absolute ethanol and clear in xylene. 5. Coverslip with a synthetic mounting media. RESULTS: Nissl granules - violet Nuclei - violet Bacteria, fungus - blue to purple Cartilage, mast cell granules - blue to purple Background - colorless Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marta Jaroszewska Sent: Tuesday, August 07, 2007 7:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] crysel violet stainning problem I'm writing because I have problem with de-staining of my samples during the staining with crysel violet. I read from the message from 2003 that the way is to dry samples in the oven, and don't use the water and alcoholes for dehydratation and differentiation. I tried to add acetic acid to alcoholes, to avoid 70, 95%...And nothing. The sections are a little bit blue after even 12 mins in crysel violet an than they are destained. I tried it with different samples, from formalin, Bouin's fixative. Finnaly, I have to try with drying in the oven but now I try to find other resolution. Crysel violet is from Sigma-Aldrich. I will appreciate for your help. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Aug 7 19:41:56 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Aug 7 19:42:22 2007 Subject: [Histonet] ASCP exams and applicants Message-ID: <001501c7d954$ec562fa0$0202a8c0@HPPav2> There have been several inquiries lately, about how a histotech trained/working in the US or in a foreign country can take the ASCP HT/HTL exam. I'll try to answer the questions in this email. So if not interested, please hit delete now. For the complete application booklet, go to: http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/def ault.aspx Click on "Download Procedures for Examination and Certification booklet in PDF format" In the US, there are two categories: - Histologic Technician, also known as Histotechnician, or HT - Histotechnologist, or HTL In each, there are two routes: - Going through a NAACLS program, which I won't cover, as each program has different requirements. For more information about HT/HTL programs, go to www.naacls.org click on find a program, and then click on HT or HTL. - Experience route, also known as on-the-job experience, or OJT For each OJT, there are three requirements: - college classes - amount of experience and type - where received training For HT, the requirement is: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. For the HTL, the requirement is: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. For both HT and HTL, the experience requirement is: To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation, Microtomy, Processing, Staining So, what does this mean? A. Degree: It doesn't have to be a degree in histotechnology, or even biology or chemistry. But make certain you have the minimum number of classes. For those from a foreign country, make certain that a 2 or 4 year degree in your country is the same in the US. Your foreign transcript will have to be evaluated by an approved company (list found in booklet and on web page). Sometimes, a 4 year degree in some foreign country is only equal to 3 years of college in the US. B. Classes: As you notice, there are no requirements as to WHICH biology and chemistry classes to take. My personal suggestions, to help understand histotechnology, would be: - HT - microbiology, anatomy, physiology, intro to biochemistry/organic chemistry - HTL - same as HT plus immunology, histology, organic chemistry, biochemistry; helpful would be genetics and/or molecular pathology - helpful to both - medical terminology, intermediate college algebra C. Experience: Must have done fixation, microtomy, processing and staining. Notice, it doesn't say all types of tissue, so if you only work on skin, that counts. If you only work with animal tissue, that counts. If you only use one fixative, that counts. If you only do frozen sections, or only paraffin sectioning, or only EM block resins, that counts. If you only do H&E, or only 1 special stain, or only IHC (or no IHC but do other special stain) - these all count. However, realize that you will be asked questions on all fixatives, processing, all stains including IHC. So you will have to study, study, study. The experience must be within the last 10 years. Yes, you can combine several labs' experiences if you have to. You will need each pathologist to sign off on your experience. D. Type of Lab Experience: The lab you are working in must be in the US, Canada, or be CAP/JCAHO accredited, in order for you to be eligible to take the HT/HTL exam. The experience must be under a ABP board certified pathologist. If the pathologist is a veterinary pathologist in the US/Canada/CAP/JCAHO, that also counts. You would need to contact ASCP BOR for more information. If you are working for a PhD in a university, it might count. Call ASCP BOR and talk with them about what type of qualifications the PhD person would need. If, however, you are working outside the US or Canada, and your lab is NOT CAP/JCAHO accredited, there is no way that you can qualify to take the exam. You would have to get a visa to work in the US, and find a US lab willing to hire you. Once you obtain the experience, then you would be able to take the HT or HTL exam (as long as you have the education, etc.). Since there is a shortage of histotechs in the US, you might be able to find a lab that is willing to help you obtain a visa, or would be willing to hire you if you have a work visa or a green card (permanent residency). I don't know how you would go about doing this. Sorry. Maybe this is where one of the companies that lists what labs are looking for histotechs might be able to help you. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From jnocito <@t> satx.rr.com Tue Aug 7 23:55:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 7 23:56:33 2007 Subject: [Histonet] Supervisor question References: <080720071550.10645.46B894C2000396F00000299522070029539C030E0B0E020A9D0E05@comcast.net> Message-ID: <003101c7d978$611be730$d49eae18@yourxhtr8hvc4p> Karen, there are no requirements for a supervisor to be certified. It is determined either by the department or the facility. JTT ----- Original Message ----- From: To: Sent: Tuesday, August 07, 2007 10:50 AM Subject: [Histonet] Supervisor question > Can anyone tell me if there is a requirement for a histology supervisor to > be certified? if so, will ASCP eligibile suffice if the HTL exam will be > taken w/in 3 years and the person has a BA degree.....thank you! > > -- > Karen Adams > Pathology Laboratories West > 9303 Park West Blvd > Knoxville, TN 37923 > (865) 690-2111 FAX (865) 691-1623 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjohnson <@t> crispregional.org Wed Aug 8 05:38:46 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Wed Aug 8 05:38:53 2007 Subject: [Histonet] I'm free! Message-ID: <4927.216.45.45.78.1186569526.squirrel@admintool.trueband.net> Please unsubscribe me. I have resigned from Crisp Regional Hospital and will no longer have access to this email address. If anyone needs a free agent for one week to one month (not affiliated with an agency). Contact me at jmjohnson34@hotmail.com. Preferably in Georgia, otherwise, I will need a plane ticket :) I am not available for the next week, I am off on a much needed vacation! From liz <@t> premierlab.com Wed Aug 8 09:41:56 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Aug 8 09:42:12 2007 Subject: [Histonet] CD44v6 and CD44v10 Message-ID: Hello All =20 Has anyone out there worked on IHC staining of different varients of = CD44 on human brain. I had what I thought was really nice staining but = the client thinks that all I am staining is lipofucsin. My negative = controls are negative and the areas that are staining do not = autofluoresce, I read some where that lipofuchsin autofluoresces so I'm = a bit confused. I do have images available if anyone would like to = review them. The antibodies are from Chemicon. Any help would be = appreciated.=20 =20 Thanks in advance =20 Liz =20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com HYPERLINK "http://www.premierlab.com/"www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.11.8/941 - Release Date: 8/7/2007 = 4:06 PM =20 From nrutledge <@t> CapeCodHealth.org Wed Aug 8 10:12:25 2007 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Wed Aug 8 10:14:29 2007 Subject: [Histonet] need VIP 3000 oerator's manual Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210210@FHEXSVR2.FHDOMAIN1.capecodhealth.org> We have old VIP 3000 as backup processor and no manual. Does anybody still have a copy? Please contact pmearle@capecodhealth.org if you can help. Thanks, Nancy Rutledge Falmouth Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential. And intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From nrutledge <@t> CapeCodHealth.org Wed Aug 8 10:34:58 2007 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Wed Aug 8 10:36:55 2007 Subject: [Histonet] Job Opportunity Falmouth, MA Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210211@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Full time histotech position at Falmouth Hospital, located on Cape Cod, MA. Small path lab, 10K surgicals per year. Day shift, full benefits, competitive salary. Contact pmearle@capecodhealth.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential. And intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From ynwang <@t> u.washington.edu Wed Aug 8 11:58:07 2007 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Wed Aug 8 12:00:12 2007 Subject: [Histonet] Nile blue sulphate - methyl green Message-ID: Hello All, I want to use the Nile blue sulphate staining method for lipids in frozen tissue. I have two questions regarding the protocol I hope you can help answer: 1)In the protocols that I have seen (e.g. Bancroft&Stevens Theory and Practice of histologican techniques) it calls for the use of chloroform washed Methyl green. After searching the histonet archives I see that methyl green should be replaced by ethyl green, or rather methyl green is no longer available. In switching out methyl green for ethyl green (I'm going for the sigma ethyl green- CI 42590), aside from not needing to wash with cholorform, are there any other protocol changes that I need to be aware of? 2)The protocols asks for frozen tissue fixed with 10% Neutral buffered Formalin or formal-calcium. Can someone tell me if there is a difference between using the two fixatives? I would prefer to use 10% NBF. Thank you for your help Yak-Nam Wang -- Research Associate CIMU Applied Physics Laboratory University of Washington Box 355640 Seattle WA 98195 From brewerd <@t> nacmem.org Wed Aug 8 12:05:29 2007 From: brewerd <@t> nacmem.org (Dana Brewer) Date: Wed Aug 8 12:06:13 2007 Subject: [Histonet] Alcohol Recycling by Gravity Message-ID: <002601c7d9de$5411faa0$0b0a10ac@work.nmh.org> I've seen lots of recommendations for CBG recylcers, but am wondering if anyone has any information (good or bad) concerning Gravity Recycling Alcohol Cartridges? Thanks so much, Dana Brewer Nacogdoches Memorial Hospital From Heather.D.Renko <@t> osfhealthcare.org Wed Aug 8 12:08:13 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Aug 8 12:08:30 2007 Subject: [Histonet] REFERENCE BOOK Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775587@pmc-rfd-mx01.intranet.osfnet.org> I agree with Ren?, Freida is a classic to have on hand. The most recent edition is Second Edition published in 1996. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Dorothy.L.Webb <@t> HealthPartners.Com Wed Aug 8 12:43:41 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Aug 8 12:43:54 2007 Subject: [Histonet] Automated microtome Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563507E@hpes1.HealthPartners.int> I am working as a supervisor at a facility that had a Leica 2155 automated microtome. Only having had experience with manual microtomes, I need to ask a question concerning the reliability that others find with these microtomes. Does everyone else who uses this particular one find it to cut all types of tissue with consistency? I only have one tech who uses this piece of equipment due to ergonomic issues and I cannot seem to ascertain if the problems that are encountered are tech related or the microtome itself. I would appreciate any feedback you could give me on your experiences with the Leica 2155, other automated microtomes on the market in comparison, etc. As always, thank you for your help!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From TreseMoles <@t> neo.rr.com Wed Aug 8 13:14:31 2007 From: TreseMoles <@t> neo.rr.com (TreseMoles@neo.rr.com) Date: Wed Aug 8 13:14:48 2007 Subject: [Histonet] AA -VS-AL Congo Red Stain Message-ID: Hello All, I recently was handed a procedure for an alcoholic Congo Red to compare AA and AL. I need clarification of the reagent prep. Is there anyone using this protocol, that could e-mail me the reagents used,and concentrations, vendors, etc. Thank You in advance- I greatly appreciate the help!!! Theresa Dobersztyn, HT ASCP Senior Technologist Akron Children's Hospital TDobersztyn@CHMCA.org From mickie25 <@t> netzero.net Wed Aug 8 13:39:24 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Aug 8 13:40:47 2007 Subject: [Histonet] Alcohol Recycling by Gravity In-Reply-To: <002601c7d9de$5411faa0$0b0a10ac@work.nmh.org> References: <002601c7d9de$5411faa0$0b0a10ac@work.nmh.org> Message-ID: These (including formalin and alcohol) from Creative Waste Solutions in Portland, Oregon and have been used for over 5 years at Sacred Heart Medical Center in Spokane and they work great. They are in use all over the country. They are very cost effective and are available in both bench and floor models. They save at least half the cost of reagent and disposal and he also have a product for keeping xylene and xylene substitutes fresh on the stainer which are very cost effective. People also rave about the ice trays they sell. The ice last for hours. Give Rex a call at: 888-795-8300 Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Brewer Sent: Wednesday, August 08, 2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcohol Recycling by Gravity I've seen lots of recommendations for CBG recylcers, but am wondering if anyone has any information (good or bad) concerning Gravity Recycling Alcohol Cartridges? Thanks so much, Dana Brewer Nacogdoches Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jb481 <@t> columbia.edu Wed Aug 8 13:47:26 2007 From: jb481 <@t> columbia.edu (Joshua Berman) Date: Wed Aug 8 13:47:35 2007 Subject: [Histonet] Flurry of questions on mounting and gelatin embedding.. Message-ID: <003f01c7d9ec$90c928c0$3926a8c0@JoshB> Thank you to all who responded with detailed suggestions about slide mounting and gelatin embedding! Joshua Berman Department of Psychiatry Columbia University From bob.nienhuis <@t> gmail.com Wed Aug 8 13:58:16 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Wed Aug 8 13:58:27 2007 Subject: [Histonet] Golgi stainembedding Message-ID: <45109da50708081158o783180f6o6bd7d74c5064cbd9@mail.gmail.com> Anybody have suggestions for alternatives to celloidin embedding for Golgi-Kopsch? Has anyone done it, and can point out some pitfalls I might avoid in doing it? I want to Golgi stain some archival human brain tissue. I have not tried it yet, and understand it can be a bit tricky. Bob nienhuis@ucla.edu From Dorothy.L.Webb <@t> HealthPartners.Com Wed Aug 8 14:04:15 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Aug 8 14:04:32 2007 Subject: [Histonet] Dehydrating beads Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635084@hpes1.HealthPartners.int> I know this was discussed in the recent past, but, what are the pros and cons of using these and how much do they save on xylene usage? Also, how does one get into the archives of Histonet to view past responses if those of you who already answered do not want to post an answer agin (and I do understand not rehashing old subjects!!) I cannot say enough how VERY much I appreciate the use of this resource for problem solving in the histology world!! The networking is invaluable and I want to thank everyone out there for the quick responses and helpful information on my last post concerning automated microtomes!!!!!! Histotechs are a truly a CUT above the rest!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From DeBrosse_Beatrice <@t> Allergan.com Wed Aug 8 14:10:42 2007 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Wed Aug 8 14:14:40 2007 Subject: [Histonet] Automated microtome Message-ID: I have a Leica 2155 and I love it. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, August 08, 2007 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated microtome I am working as a supervisor at a facility that had a Leica 2155 automated microtome. Only having had experience with manual microtomes, I need to ask a question concerning the reliability that others find with these microtomes. Does everyone else who uses this particular one find it to cut all types of tissue with consistency? I only have one tech who uses this piece of equipment due to ergonomic issues and I cannot seem to ascertain if the problems that are encountered are tech related or the microtome itself. I would appreciate any feedback you could give me on your experiences with the Leica 2155, other automated microtomes on the market in comparison, etc. As always, thank you for your help!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judi.ford <@t> roche.com Wed Aug 8 14:46:58 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Wed Aug 8 14:47:16 2007 Subject: [Histonet] Masson's Trichrome Troubleshooting Message-ID: Hi everyone, I'm hoping someone can give me suggestions on what happened to my stain. I did a Masson's Trichrome on mouse hearts/aortas this morning. I followed the AFIP protocol exactly and used two different control slides (tongue, aorta, skeletal muscle). We have previously stained control slides with the tissue I used this time and they worked beautifully. After finishing the glacial acetic acid rinse I examined the slides under the scope and found that neither of the control slides stained with the aniline blue, but all the study slides stained beautifully with aniline blue. I tried going back and restaining (from phosphomolybdic/phosphotungstic acid) the slides and the same results showed. I thought maybe I had them in the glacial acetic acid too long so I cut that back to 3 minutes instead of 5 for the second round. Any ideas on what could have happened? Could the control slides have been sitting too long as unstained slides? Could the aniline blue working solution need an extra punch from glacial acetic acid? In the past I've reused aniline blue without any problems and this solution wasn't past its expiration date. Would love to hear your thoughts...... Judi Ford Palo Alto, CA From paul.kammeyer <@t> comphealth.com Wed Aug 8 14:57:36 2007 From: paul.kammeyer <@t> comphealth.com (paul.kammeyer@comphealth.com) Date: Wed Aug 8 14:53:15 2007 Subject: [Histonet] Job Opportunities Message-ID: After seeing all of the excitement about job postings last week I decided to post a few of my jobs. :) I would love to help you out in any way possible. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Temporary Assignments: These jobs are ready to be filled! - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1. Oregon - Northern - Histotech 3rd shift 2. Texas - Northeast - Histotech 3rd shift (2 positions) 3. Virginia - Eastern - Histotech 1st shift 4. Texas - Northeast - Histotech 1st shift (1 position) 3rd (1 position) - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Permanent Positions: We have positions in the following states. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1. Florida 2. Iowa 3. Nevada 4. Oregon 5. Rhode Island 6. South Carolina 7. Wisconsin My name is Paul and if you have any questions about any of the above positions don't hesitate to give me a call. I would love to answer any questions you may have or find you a position in a different location. Also, if you would like to refer someone looking for different opportunities you could earn a $500 bonus. Call us for staffing help. Thank you! Paul Kammeyer Staffing Consultant Lab Specialties - CompHealth P.O. Box 713400 Salt Lake City, UT 84171-3400 Phone: (800) 447-6016 ext. 3380 Fax: (866) 588-1340, (801) 930-4504 paul.kammeyer@comphealth.com www.comphealth.com From themagoos <@t> rushmore.com Wed Aug 8 14:59:20 2007 From: themagoos <@t> rushmore.com (themagoos) Date: Wed Aug 8 14:59:30 2007 Subject: [Histonet] Hale Colloidal Iron Procedure Message-ID: <46ba2098.382.2a8f.1823546904@rushmore.com> One of my pathologist is wanting to do a Hale Colloidal iron for Chromophobe Renal Cell Carcinoma. Does anybody have a procedure for this? Or is there other special stains/ IHC that demonstrate this? Thank you in advance for your responses. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com From gcallis <@t> montana.edu Wed Aug 8 15:23:43 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Aug 8 15:23:54 2007 Subject: [Histonet] Hales Colloidal iron Message-ID: <6.0.0.22.1.20070808141702.01b02cc0@gemini.msu.montana.edu> You should also look in the Histonet archives for this, there were several discussions in 1999, 2002 and 2003. The Muller/Mowry's or Mowry's modification of Hales should work, and is found in Frieda Carsons book. This was posted on Histonet in 2002 and found in Histonet Archives From:Bryan Llewellyn ---------- According to Pearse's Histochemistry: Theoretical and applied, Hales colloidal iron is as follows: Fix in Wolman, Carnoy or formalin. Paraffin sections. Avoid adhesives. 1. Sections to water. 2. Mix 1 vol dialysed iron and 1 vol 2M acetic acid. Apply for 10 mins. 3. Wash very well with distilled water to remove all traces of iron. 4. Do Perls' prussian blue. 5. Wash well with water. 6. Counterstain with neutral red 1 min. 7. Dehydrate, clear, mount Acid mucins - blue Nuclei - red Rinehart and Abu'l Haj dialysed iron 75 g ferric chloride 250 mL water Dissolve and add 100 mL glycerol. While stirring, add 55 mL strong ammonia. Dialyse against distilled water for three days, changing the water regularly. This mixture triples in volume by the end of dialysis. ----- Original Message ----- From: "Goodwin, Diana" To: "'Histonet'" Sent: Thursday, April 04, 2002 8:16 AM Subject: Hale's method for colloidal iron > Greetings, Netters.> > Does anyone have a copy of Hale's method for colloidal iron? My pathologist has asked for it specifically, probably because it is cited in the literature for differentiation of chromophobe RCC. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From TJasper <@t> smdc.org Wed Aug 8 15:29:16 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Wed Aug 8 15:29:37 2007 Subject: [Histonet] Automated microtome In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270563507E@hpes1.HealthPartners.int> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A61D@SCREECH.ntcampus.smdc.org> Dorothy, I'd have no qualms about the Leica 2155, we've got one here and it's a gem. Also you are in a great area for service, North Central Instruments - Curt Westberg, et al. I mention the service since the only complaint I've heard (in another part of the country was about Leica service, or the lack thereof). Unfortunately, this lack of reliable service was the deciding factor against obtaining a Leica 2155 for a path group I know. Bottom line...the unit is great, combine that with good service and everyone's happy. Thomas Jasper HT (ASCP) BAS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Webb, Dorothy L Sent: Wednesday, August 08, 2007 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated microtome I am working as a supervisor at a facility that had a Leica 2155 automated microtome. Only having had experience with manual microtomes, I need to ask a question concerning the reliability that others find with these microtomes. Does everyone else who uses this particular one find it to cut all types of tissue with consistency? I only have one tech who uses this piece of equipment due to ergonomic issues and I cannot seem to ascertain if the problems that are encountered are tech related or the microtome itself. I would appreciate any feedback you could give me on your experiences with the Leica 2155, other automated microtomes on the market in comparison, etc. As always, thank you for your help!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From JWEEMS <@t> sjha.org Wed Aug 8 15:28:55 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 8 15:29:59 2007 Subject: [Histonet] Hale Colloidal Iron Procedure In-Reply-To: <46ba2098.382.2a8f.1823546904@rushmore.com> References: <46ba2098.382.2a8f.1823546904@rushmore.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320464BD02@sjhaexc02.sjha.org> Do a search in the archives. I found it just the other day! Let me know if it doesn't work and I'll see if I can help.. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of themagoos Sent: Wednesday, August 08, 2007 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hale Colloidal Iron Procedure One of my pathologist is wanting to do a Hale Colloidal iron for Chromophobe Renal Cell Carcinoma. Does anybody have a procedure for this? Or is there other special stains/ IHC that demonstrate this? Thank you in advance for your responses. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Aug 8 15:31:00 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 8 15:32:07 2007 Subject: [Histonet] Hales Colloidal iron In-Reply-To: <6.0.0.22.1.20070808141702.01b02cc0@gemini.msu.montana.edu> References: <6.0.0.22.1.20070808141702.01b02cc0@gemini.msu.montana.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320464BD04@sjhaexc02.sjha.org> Oh well, nevermind! Thanks Gayle... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, August 08, 2007 3:24 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hales Colloidal iron You should also look in the Histonet archives for this, there were several discussions in 1999, 2002 and 2003. The Muller/Mowry's or Mowry's modification of Hales should work, and is found in Frieda Carsons book. This was posted on Histonet in 2002 and found in Histonet Archives From:Bryan Llewellyn ---------- According to Pearse's Histochemistry: Theoretical and applied, Hales colloidal iron is as follows: Fix in Wolman, Carnoy or formalin. Paraffin sections. Avoid adhesives. 1. Sections to water. 2. Mix 1 vol dialysed iron and 1 vol 2M acetic acid. Apply for 10 mins. 3. Wash very well with distilled water to remove all traces of iron. 4. Do Perls' prussian blue. 5. Wash well with water. 6. Counterstain with neutral red 1 min. 7. Dehydrate, clear, mount Acid mucins - blue Nuclei - red Rinehart and Abu'l Haj dialysed iron 75 g ferric chloride 250 mL water Dissolve and add 100 mL glycerol. While stirring, add 55 mL strong ammonia. Dialyse against distilled water for three days, changing the water regularly. This mixture triples in volume by the end of dialysis. ----- Original Message ----- From: "Goodwin, Diana" To: "'Histonet'" Sent: Thursday, April 04, 2002 8:16 AM Subject: Hale's method for colloidal iron > Greetings, Netters.> > Does anyone have a copy of Hale's method for colloidal iron? My pathologist has asked for it specifically, probably because it is cited in the literature for differentiation of chromophobe RCC. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Wed Aug 8 16:11:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Aug 8 16:11:38 2007 Subject: [Histonet] Automated microtome In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A61D@SCREECH.ntcampus. smdc.org> References: <0E394B648E5284478A6CCB78E5AFDA270563507E@hpes1.HealthPartners.int> <1A9F2A6C5762524799A816F1F09744CF0143A61D@SCREECH.ntcampus.smdc.org> Message-ID: <6.0.0.22.1.20070808150921.01b51dc0@gemini.msu.montana.edu> I have never had lack of reliable service from Leica. And I think it is better than ever. At 02:29 PM 8/8/2007, you wrote: >Dorothy, > >I'd have no qualms about the Leica 2155, we've got one here and it's a >gem. Also you are in a great area for service, North Central Instruments >- Curt Westberg, et al. I mention the service since the only complaint >I've heard (in another part of the country was about Leica service, or the >lack thereof). Unfortunately, this lack of reliable service was the >deciding factor against obtaining a Leica 2155 for a path group I know. >Bottom line...the unit is great, combine that with good service and >everyone's happy. > >Thomas Jasper HT (ASCP) BAS Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From jlyoder <@t> mail2jason.com Wed Aug 8 16:44:05 2007 From: jlyoder <@t> mail2jason.com (jason yoder) Date: Wed Aug 8 16:44:57 2007 Subject: [Histonet] NSH Information packets for Denver Message-ID: <4c7301c7da05$3deea490$016a010a@mail2world.com> Hi All, Has anyone recieved information from NSH regarding the symposium in Denver? Thanks, Jason Yoder Click here to find the right stock, bonds, and mutual funds.

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Unlimited Email Storage – POP3 – Calendar – SMS – Translator – Much More! From Jason.Wiese <@t> va.gov Wed Aug 8 17:06:23 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Wed Aug 8 17:06:45 2007 Subject: [Histonet] NSH Information packets for Denver In-Reply-To: <4c7301c7da05$3deea490$016a010a@mail2world.com> References: <4c7301c7da05$3deea490$016a010a@mail2world.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12BB4@VHAV20MSGA3.v20.med.va.gov> I have received my packet and already registered for the convention and reserved rooms. Here is some contact info for you... Brenda Royce Registration Coordinator National Society of Histotechnology 10320 Little Patuxent Parkway, Suite 804 Columbia, MD 21044 (443) 535-4060 (443) 535-4055 (Fax) Best Regards, Jason Wiese, BS, CJ, HT, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jason yoder Sent: Wednesday, August 08, 2007 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Information packets for Denver Hi All, Has anyone recieved information from NSH regarding the symposium in Denver? Thanks, Jason Yoder Click here to find the right stock, bonds, and mutual funds.

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Unlimited Email Storage – POP3 – Calendar – SMS – Translator – Much More! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Aug 8 17:18:39 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Aug 8 17:18:44 2007 Subject: [Histonet] NSH Information packets for Denver In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12BB4@VHAV20MSGA3.v20.med .va.gov> References: <4c7301c7da05$3deea490$016a010a@mail2world.com> <70EEF3D43B3C164C94037D811B2BE193E12BB4@VHAV20MSGA3.v20.med.va.gov> Message-ID: <6.0.0.22.1.20070808161633.01b32668@gemini.msu.montana.edu> The website at www.nsh.org has all the information too. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 04:06 PM 8/8/2007, you wrote: >I have received my packet and already registered for the convention and >reserved rooms. >Here is some contact info for you... > >Brenda Royce > >Registration Coordinator > >National Society of Histotechnology > >10320 Little Patuxent Parkway, Suite 804 > >Columbia, MD 21044 > >(443) 535-4060 (443) 535-4055 (Fax) > > > > >Best Regards, >Jason Wiese, BS, CJ, HT, PA(ASCP) > > > > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jason >yoder >Sent: Wednesday, August 08, 2007 2:44 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] NSH Information packets for Denver > >Hi All, >Has anyone recieved information from NSH regarding the symposium in >Denver? > >Thanks, >Jason Yoder > > >Click here to find the right stock, bonds, and mutual funds. >zq8TXossdmZ3cJr/> > > > >

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Get the Free email that has everyone talking at href=http://www.mail2world.com >target=new>http://www.mail2world.com
color=#999999>Unlimited Email Storage – POP3 – Calendar – >SMS – Translator – Much More! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marshall.Kulkin <@t> cshs.org Wed Aug 8 17:34:14 2007 From: Marshall.Kulkin <@t> cshs.org (Kulkin, Marshall) Date: Wed Aug 8 17:35:29 2007 Subject: Recall: [Histonet] Automated microtome Message-ID: Kulkin, Marshall would like to recall the message, "[Histonet] Automated microtome". IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From jnocito <@t> satx.rr.com Wed Aug 8 17:57:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 8 17:58:17 2007 Subject: [Histonet] Dehydrating beads References: <0E394B648E5284478A6CCB78E5AFDA2705635084@hpes1.HealthPartners.int> Message-ID: <003101c7da0f$8a23b8f0$d49eae18@yourxhtr8hvc4p> Dorothy, I tried these dehydrating beads and really didn't like them (okay, let the flaming begin, I'm sure I just POed someone. This time, I'm not working haaaaaaaa) Sorry, I tried them at two different labs and wasn't really impressed. You have to keep track of how many trays (or slides) go through before you need to rotate them and let them dry out completely before reusing them again. I would try the gravity method like Mickey suggested. JTT ----- Original Message ----- From: "Webb, Dorothy L" To: Sent: Wednesday, August 08, 2007 2:04 PM Subject: [Histonet] Dehydrating beads I know this was discussed in the recent past, but, what are the pros and cons of using these and how much do they save on xylene usage? Also, how does one get into the archives of Histonet to view past responses if those of you who already answered do not want to post an answer agin (and I do understand not rehashing old subjects!!) I cannot say enough how VERY much I appreciate the use of this resource for problem solving in the histology world!! The networking is invaluable and I want to thank everyone out there for the quick responses and helpful information on my last post concerning automated microtomes!!!!!! Histotechs are a truly a CUT above the rest!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Wed Aug 8 18:12:48 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Aug 8 18:13:00 2007 Subject: [Histonet] NSH catalog Message-ID: <915448.71738.qm@web50612.mail.re2.yahoo.com> Am I the only one that hasn't received the NSH catalog for the symposium in Denver, or do we have to get it on line now? Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From melissa.mazan <@t> tufts.edu Wed Aug 8 18:16:29 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Wed Aug 8 18:16:45 2007 Subject: [Histonet] immunostaining post-FACS Message-ID: <20070808191629.vfq6wu4h6o0kccko@webmail.tufts.edu> Hi all, Wondering if anyone has done much immunostaining post FACS. WE have had a lot of trouble with this procedure - we collect our cells (from murine lung) into BSA on ice, bring them back to our lab (FACS is at our core facility, so it means about an hour and a half before the cells get to our lab). In the lab, we immediately cytospin - the morphology is nice on H and E - and then fix in Cytofix Cytoperm for 20 minutes at 4C. We either commence immunostaining immediately after, or leave in fridge for the next day. We do either immunofluorescence or immunoenzyme (usually ABC system) for identifying antigens of interest. We always have a negative control, but don't always have a positive control. We do either double staining for SPC and CC10 or single staining for alpha sma, vimentin, e-cad, or pancytokeratin. The problem that we have is that although the negative controls are very negative with respect to the cases, we seem to be getting a lot of false positives when the antibody is actually applied - for instance, my cells will stain 80% positive - strongly positive - using alpha sma, but a Western of the same cells will show no alpha-sma at all, whereas there is positive staining on whole lung suspensions. Any ideas? Advice for getting good post-FACS immunostaining? Many thanks - Melissa Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu From ryan <@t> upei.ca Wed Aug 8 18:33:05 2007 From: ryan <@t> upei.ca (ryan@upei.ca) Date: Wed Aug 8 18:38:04 2007 Subject: [Histonet] Histonet Digest, Vol 45, Issue 12 Message-ID: <8C0B2B7720B@acad1.cs.upei.ca> C.L. Ryan will be out of the office from Friday Aug 10 until Monday Aug. 13, incl. From mtarango <@t> nvcancer.org Wed Aug 8 19:08:08 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Aug 8 19:18:08 2007 Subject: [Histonet] immunostaining post-FACS In-Reply-To: <20070808191629.vfq6wu4h6o0kccko@webmail.tufts.edu> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6DB8@NVCIEXCH02.NVCI.org> These cells haven't already been stained and sorted or something, have they? If so, I would assume that whatever antibodies they used for flow/FACS will be on the cells. You said you use cytofix/cytoperm, that might denature the flow antibodies (if there are any), but you never know. Have you tried doing the cytofix/cytoperm on the cells while they are in suspension, and THEN making the cytospins? Switching to a different fixative? Gayle Callis had a post a while ago about using a fixative of 75% acetone and 25% methanol for murine CD markers. Maybe it would work for all your markers. I've tried modifying this fixative using propanol instead of ethanol for frozen sections on human tissue. It worked. When all else fails, I like to, as closely as possible, treat the specimen like formalin-fixed paraffin-embedded tissue. I will fix in formalin, dehydrate in staining dishes (like processing tissue) ...then bring it back to water and do antigen retrieval before staining. You might lose too many cells trying this on cytospins though, but might as well throw it out there. Just some thoughts... Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Mazan Sent: Wednesday, August 08, 2007 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immunostaining post-FACS Hi all, Wondering if anyone has done much immunostaining post FACS. WE have had a lot of trouble with this procedure - we collect our cells (from murine lung) into BSA on ice, bring them back to our lab (FACS is at our core facility, so it means about an hour and a half before the cells get to our lab). In the lab, we immediately cytospin - the morphology is nice on H and E - and then fix in Cytofix Cytoperm for 20 minutes at 4C. We either commence immunostaining immediately after, or leave in fridge for the next day. We do either immunofluorescence or immunoenzyme (usually ABC system) for identifying antigens of interest. We always have a negative control, but don't always have a positive control. We do either double staining for SPC and CC10 or single staining for alpha sma, vimentin, e-cad, or pancytokeratin. The problem that we have is that although the negative controls are very negative with respect to the cases, we seem to be getting a lot of false positives when the antibody is actually applied - for instance, my cells will stain 80% positive - strongly positive - using alpha sma, but a Western of the same cells will show no alpha-sma at all, whereas there is positive staining on whole lung suspensions. Any ideas? Advice for getting good post-FACS immunostaining? Many thanks - Melissa Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From jkiernan <@t> uwo.ca Wed Aug 8 23:01:04 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Aug 8 23:01:16 2007 Subject: [Histonet] Masson's Trichrome Troubleshooting In-Reply-To: References: Message-ID: If the known positive control slides were side-by side with the study slides (which stained correctly), then there must be something wrong with the control slides, not the solutions or the steps of the method. Different fixation or inadequate removal of paraffin come to mind, but you've probably thought of those. Your study slides of heart etc were well stained, so perhaps this will be a suitable positive control for future Masson stainings. It would be satisfying, however, to know why previously positive collagen in your sections of muscle etc failed to stain with aniline blue. Was the collagen in these sections red or not stained at all? If it was red, then the PTA/PMA had failed to displace the red dye, and a longer time in PTA/PMA is likely to be needed. If that's the case the control sections are unsuitable for staining beside your study sections. The final wash is in dilute acetic acid to prevent the removal of any bound red or blue dye, which can occur if plain water is used. You rightly examined the wet sections at this stage, an action that shows that you know what you are about, unlike many professors, postdocs, graduate students, . . . Please let us all know if you find out why the collagen in previously good positive-control tissue became unstainable with the Masson's method in Luna's AFIP manual. John A. Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada N6A 5C1 http://publish.uwo.ca/~jkiernan/index.htm --- ----- Original Message ----- From: "Ford, Judi" Date: Wednesday, August 8, 2007 15:48 Subject: [Histonet] Masson's Trichrome Troubleshooting To: histonet@lists.utsouthwestern.edu > Hi everyone, > > > > I'm hoping someone can give me suggestions on what happened to > my stain. > I did a Masson's Trichrome on mouse hearts/aortas this > morning. I > followed the AFIP protocol exactly and used two different > control slides > (tongue, aorta, skeletal muscle). We have previously stained control > slides with the tissue I used this time and they worked beautifully. > > > > After finishing the glacial acetic acid rinse I examined the slides > under the scope and found that neither of the control slides stained > with the aniline blue, but all the study slides stained > beautifully with > aniline blue. I tried going back and restaining (from > phosphomolybdic/phosphotungstic acid) the slides and the same results > showed. I thought maybe I had them in the glacial acetic acid > too long > so I cut that back to 3 minutes instead of 5 for the second round. > > > > Any ideas on what could have happened? Could the control slides have > been sitting too long as unstained slides? Could the aniline blue > working solution need an extra punch from glacial acetic acid? > In the > past I've reused aniline blue without any problems and this solution > wasn't past its expiration date. > > > > Would love to hear your thoughts...... > > > > Judi Ford > > Palo Alto, CA > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Thu Aug 9 00:00:14 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Aug 9 00:00:19 2007 Subject: [Histonet] Golgi stainembedding Message-ID: Dear Bob N. If your archived tissue is in formaldehyde you probably will not be able to get Golgi preparations that are good enough for research purposes. There are plenty of published Golgi techniques for ideally fixed material (up to a few weeks). Researchers demanding perfect dendritic morphology (for counting dendritic spines, or making mathematical models of branching) often fix their specimens in Cox's solution and apply the alkaline developer to thick sections cut with a vibrating microtome (Vibratome or similar). This can provide superb Golgi preparations, even with the brains of adult animals. Kolb, at Lethbridge University, is a major proponent of these methods. His publications should be available in your library. Try Scopus or Web of Science. For good Golgi preparations you need to work closely with someone experienced with the various techniques and you must become familiar with the technical literature. Two books have chapters that serve as introductory reading: Bradley, PB (ed) 1975. Methods in Brain Research . Wiley. Santini, M. 1975. Golgi Centennial Symposium. Raven Press. There are two families of Golgi methods. The intracellular chromate ions may be precipitated by silver or mercury, and the results are not the same. I wish you well with obtaining good Golgi preps on fixed human brains. If you perfect the technique you will have the makings of a significant publication. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada. --- ----- Original Message ----- From: Bob Nienhuis Date: Wednesday, August 8, 2007 14:59 Subject: [Histonet] Golgi stainembedding To: histonet@lists.utsouthwestern.edu > Anybody have suggestions for alternatives to celloidin embedding > for Golgi-Kopsch? Has anyone done it, and can point out some > pitfalls I might avoid in doing it? I want to Golgi stain some > archival human brain tissue. > > I have not tried it yet, and understand it can be a bit tricky. > > Bob > nienhuis@ucla.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dlcowie <@t> prodigy.net Thu Aug 9 07:35:20 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Thu Aug 9 07:35:35 2007 Subject: [Histonet] change in email address Message-ID: <771388.95993.qm@web81007.mail.mud.yahoo.com> Dear Histonet, please change my email address for receiving messages to Dawn_Cowie@yahoo.com thanks, Dawn From LINDA.MARGRAF <@t> childrens.com Thu Aug 9 08:02:05 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Aug 9 08:02:31 2007 Subject: [Histonet] change in email address In-Reply-To: <771388.95993.qm@web81007.mail.mud.yahoo.com> References: <771388.95993.qm@web81007.mail.mud.yahoo.com> Message-ID: <46BAC9FD020000DA000113A0@CNET3.CHILDRENS.COM> Dear Dawn (and other Histonetters): I will change your address on the Histonet email address list as you requested but I wanted to point out to you and all the other Histonet members that you can change your own address or change other features of your subscription by going to the website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and using the instructions at the bottom of the page. People can switch to digest mode, temporarily stop messages while they go on vacation etc. If you cannot remember your password you can request it there too. For anyone having trouble posting messages because the server says you are not a member, this likely means your address has changed somewhat from when you subscribed (a common occurrence in institutional email systems) and you need to update your address which you can do there. By the way Histonet is up tp >2600 members now! Thanks, Linda M Histonet administrator >>> Dawn Cowie 08/09/07 7:35 AM >>> Dear Histonet, please change my email address for receiving messages to Dawn_Cowie@yahoo.com thanks, Dawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Thu Aug 9 08:12:29 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Aug 9 08:12:57 2007 Subject: [Histonet] NSH catalog In-Reply-To: <915448.71738.qm@web50612.mail.re2.yahoo.com> References: <915448.71738.qm@web50612.mail.re2.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBEF3@LTA3VS003.ees.hhs.gov> I received mine a few weeks ago. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Wednesday, August 08, 2007 7:13 PM To: Histonet Subject: [Histonet] NSH catalog Am I the only one that hasn't received the NSH catalog for the symposium in Denver, or do we have to get it on line now? Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aubrey <@t> nsh.org Thu Aug 9 08:26:15 2007 From: aubrey <@t> nsh.org (Aubrey Wanner) Date: Thu Aug 9 08:26:36 2007 Subject: [Histonet] NSH Symposium/Convention Message-ID: The 33rd Annual NSH Symposium/Convention will take place in Denver, October 26-31. Registration programs mailed in May - if you did not receive a copy please feel free to contact the NSH Office, 443-535-4060 or visit the website and click on Meetings/Events > Symposium/Convention. All workshops are still available and we still have rooms in the headquarters hotel. Any questions please call our office - we hope to see you there! Mrs. Aubrey M.J. Wanner Meeting Manager, Annual Symposium/Convention Managing Editor, Journal of Histotechnology We've Moved! National Society for Histotechnology 10320 Little Patuxent Parkway | Suite 804 Columbia, MD 21044 P | 443.535.4060 Direct | 443.535.4065 F | 443-535-4055 http://www.nsh.org/ From jnocito <@t> satx.rr.com Thu Aug 9 08:40:05 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 9 08:40:40 2007 Subject: [Histonet] change in email address References: <771388.95993.qm@web81007.mail.mud.yahoo.com> <46BAC9FD020000DA000113A0@CNET3.CHILDRENS.COM> Message-ID: <000b01c7da8a$ccd435f0$d49eae18@yourxhtr8hvc4p> WOW! I think it's time again to thank Dr. M and her staff for maintaining the Histonet. Party in Denver!? ----- Original Message ----- From: "LINDA MARGRAF" To: "histonet" ; "Dawn Cowie" Sent: Thursday, August 09, 2007 8:02 AM Subject: Re: [Histonet] change in email address Dear Dawn (and other Histonetters): I will change your address on the Histonet email address list as you requested but I wanted to point out to you and all the other Histonet members that you can change your own address or change other features of your subscription by going to the website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and using the instructions at the bottom of the page. People can switch to digest mode, temporarily stop messages while they go on vacation etc. If you cannot remember your password you can request it there too. For anyone having trouble posting messages because the server says you are not a member, this likely means your address has changed somewhat from when you subscribed (a common occurrence in institutional email systems) and you need to update your address which you can do there. By the way Histonet is up tp >2600 members now! Thanks, Linda M Histonet administrator >>> Dawn Cowie 08/09/07 7:35 AM >>> Dear Histonet, please change my email address for receiving messages to Dawn_Cowie@yahoo.com thanks, Dawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Annette_hall <@t> pa-ucl.com Thu Aug 9 08:41:50 2007 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Thu Aug 9 08:42:18 2007 Subject: [Histonet] Slide Staining Variability Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B6054@mercury.pa-ucl.com> Histonetters, For those of you who use automated IHC staining systems, has anyone had issues with part of the slide staining and the rest of the slide not? We have adopted the policy of placing the control on with the patient tissue as a more comprehensive check of the system. However, we occasionally find the control is acceptable but the patient fails to even counterstain. This has been an intermittent problem on the Ventana Benchmark for some time, but the frequency has been increasing to almost daily. Any thoughts? Thanks, Annette J Hall Histo/Cyto/Micro Supervisor United Clinical Laboratories Dubuque, IA 52001 -----Original Message----- From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] Sent: Monday, August 06, 2007 3:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer w/integrated coverslipper Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From joost.bruijntjes <@t> tno.nl Thu Aug 9 09:34:53 2007 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Thu Aug 9 09:35:16 2007 Subject: [Histonet] (no subject) Message-ID: <8865601DD17A554CB489C17FFD8A51B248507E@MAIL04.tsn.tno.nl> Please, change my email address into joost.bruijntjes@tno.nl Thanks TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From JCollins <@t> palmbeachpath.com Thu Aug 9 09:35:05 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Thu Aug 9 09:35:18 2007 Subject: [Histonet] Slide Staining Variability Message-ID: With the Ventana system, staining variability on different parts of the slide usually is a result of a problem with the vortex mixers, although other issues such as dispensers not working properly can cause this as well. You can run a test on the vortex mixers (Find the procedure in the online manual). You can also call their technical support line for assistance. Judy Collins From gcallis <@t> montana.edu Thu Aug 9 09:50:49 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Aug 9 09:51:00 2007 Subject: Ethanol, never methanol for CD marker fixation RE: [Histonet] immunostaining post-FACS In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE011B6DB8@NVCIEXCH02.NVCI.o rg> References: <20070808191629.vfq6wu4h6o0kccko@webmail.tufts.edu> <5AEC610C1CE02945BD63A395BA763EDE011B6DB8@NVCIEXCH02.NVCI.org> Message-ID: <6.0.0.22.1.20070809082551.01b37dd8@gemini.msu.montana.edu> Dear Mark and Melissa,' A correction to Marks reply. I do NOT use methanol in my acetone/alcohol fixative. The fixative is made up with absolute ethanol 75% acetone/25% absolute ETHANOL. Methanol ruins my CD markers (in the literature and also in Histonet archives) . However, this acetone alcohol fixative is not good with human CD4 and CD8 markers since they do not like ethanol either. And there are antibodies that will work best with formalin or paraformaldehyde fixation. Could it be the antigens you are trying to stain do not like the fixative you are using? I suggest you do a fixation panel on known positive cells to optimize the fixation. That positive control is important to know IF your antibodies, and method is working. A known positive tissue control may help too, then you know your antibodies are working on mouse tissues, cells. Frozen sections and different fixatives should provide some answers. Some antibodies work better on Western blots, but when you try to use them on tissue sections (or at the same concentration as the blot) you get nothing. Also, we try to purchase antibodies known to work on cells/tissue sections, and avoid just the Western Blot application. Double check your BSA, and make sure it is protease/immunoglobulin free, Jackson has this - there is an interesting publication on albumin causing background staining in AIMM journal, titled Albumin in Immuhohistochemistry: Foe or Friend? 14(4l ), December 2006 Mittelbronn M et al. I do have the publication in pdf form if you would like to read it. I am curious and as Mark pointed out - to sort the cells, are you staining those with an antibody, sort, collect, then stain with another antibody? I was a bit lost on this. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 06:08 PM 8/8/2007, you wrote: >These cells haven't already been stained and sorted or something, have >they? If so, I would assume that whatever antibodies they used for >flow/FACS will be on the cells. You said you use cytofix/cytoperm, that >might denature the flow antibodies (if there are any), but you never >know. > >Have you tried doing the cytofix/cytoperm on the cells while they are in >suspension, and THEN making the cytospins? Switching to a different >fixative? Gayle Callis had a post a while ago about using a fixative of >75% acetone and 25% methanol for murine CD markers. Maybe it would work >for all your markers. I've tried modifying this fixative using propanol >instead of ethanol for frozen sections on human tissue. It worked. > >When all else fails, I like to, as closely as possible, treat the >specimen like formalin-fixed paraffin-embedded tissue. I will fix in >formalin, dehydrate in staining dishes (like processing tissue) ...then >bring it back to water and do antigen retrieval before staining. You >might lose too many cells trying this on cytospins though, but might as >well throw it out there. > > >Just some thoughts... > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa >Mazan >Sent: Wednesday, August 08, 2007 4:16 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] immunostaining post-FACS > >Hi all, Wondering if anyone has done much immunostaining post FACS. WE >have had a lot of trouble with this procedure - we collect our cells >(from murine lung) into BSA on ice, bring them back to our lab (FACS >is at our core facility, so it means about an hour and a half before >the cells get to our lab). In the lab, we immediately cytospin - the >morphology is nice on H and E - and then fix in Cytofix Cytoperm for 20 >minutes at 4C. We either commence immunostaining immediately after, or >leave in fridge for the next day. We do either immunofluorescence or >immunoenzyme (usually ABC system) for identifying antigens of interest. > We always have a negative control, but don't always have a positive >control. We do either double staining for SPC and CC10 or single >staining for alpha sma, vimentin, e-cad, or pancytokeratin. The >problem that we have is that although the negative controls are very >negative with respect to the cases, we seem to be getting a lot of >false positives when the antibody is actually applied - for instance, >my cells will stain 80% positive - strongly positive - using alpha sma, >but a Western of the same cells will show no alpha-sma at all, whereas >there is positive staining on whole lung suspensions. Any ideas? >Advice for getting good post-FACS immunostaining? Many thanks - Melissa > >Melissa R. Mazan, DVM, Diplomate ACVIM >Associate Professor and Director of Equine Sports Medicine >Department of Clinical Sciences >Tufts Cumming School of Veterinary Medicine >200 Westborough Road >North Grafton, MA 01536 >Tel:508-839-5395 >Fax:508-839-7922 >email: melissa.mazan@tufts.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >------------------------------------------------------------------------------ >CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, >and/or privileged information protected by law. If you are >not the intended recipient, you may not use, copy, or >distribute this e-mail message or its attachments. If you >believe you have received this e-mail message in error, >please contact the sender by reply e-mail and destroy all >copies of the original message >============================================================================== > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From mpence <@t> grhs.net Thu Aug 9 09:59:47 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 9 10:00:21 2007 Subject: [Histonet] Slide Staining Variability In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C012B6054@mercury.pa-ucl.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6AE@IS-E2K3.grhs.net> We have had some of these same problems and it is always been a nozzle or mixer problem. Do you perform regular PM's? The reaction buffer ph could also be off, that can cause varied staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Hall Sent: Thursday, August 09, 2007 8:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Staining Variability Histonetters, For those of you who use automated IHC staining systems, has anyone had issues with part of the slide staining and the rest of the slide not? We have adopted the policy of placing the control on with the patient tissue as a more comprehensive check of the system. However, we occasionally find the control is acceptable but the patient fails to even counterstain. This has been an intermittent problem on the Ventana Benchmark for some time, but the frequency has been increasing to almost daily. Any thoughts? Thanks, Annette J Hall Histo/Cyto/Micro Supervisor United Clinical Laboratories Dubuque, IA 52001 -----Original Message----- From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] Sent: Monday, August 06, 2007 3:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer w/integrated coverslipper Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Aug 9 10:08:11 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Aug 9 10:08:22 2007 Subject: [Histonet] Region II Fall Seminar for September 6, 7 and 8, 2207 Message-ID: <6.2.5.6.2.20070809110548.01c7c128@vet.upenn.edu> We are moving close to the dates for the meeting and registration is coming so please look over the program and request your full program if you don't have it. The following is a list of our topics and speakers. Please let us know if you need more information. The meeting is at the Delaware Technical Community College in Stanton DE. It is just off I-95 with easy access. The Thursday is primarily slated for management issues and topics while the remainder of Friday and Saturday are general topics for histology, cytology and research. If you CEUs and can't get to Denver this is an option for you to catch up this year. Region II Fall Symposium, September 6th, 7th, 8th 2007 Delaware Technical Community College-Stanton, DE Can?t get to Denver for the NSH Symposium and Convention yet still need CEU?s? Join us for the Region II Fall Symposium in Delaware! Thursday Seminars*^ - will be geared toward management topics. 8:30 am to 11:30 am WS #01 CPT Coding-How Do I Code This One? Bonnie Whitaker 3 hrs WS #02 CAP Inspection-What?s New and Required Now Charlie Dorner 3 hrs 1:00 pm to 4:30 pm WS #03 Lean ? Organizing Your Life and Lab Donna Montegue 3 hrs 1:00 pm to 2:30 pm WS #04 Reducing Immunohistochemistry Expenses Joe Myers 1.5 hrs 3:00 pm to 4:30 pm WS #05 Important Consideration in Selection of Joe Myers 1.5 hrs IHC Instruments Friday Seminars*^ 8:30 am to 12:00 pm WS #06 Beginning IHC (new to the field or refresher) Bonnie Whitaker 3 hrs 8:30 am to 10:00 am WS #07 Delaware Coroner Cases Dr. Richard Callery 1.5 hrs WS #08 Brain Diseases and Identification by Histology Dr. Barbara Crain 1.5 hrs WS #09 Sentinel Nodes & Breast Biopsies Dr. Mary Iococca 1.5 hrs 10:30 am to 12:00 pm WS #10 Tumor Classification Dr. Gary Witkin 1.0 hrs WS #11 Deconvolution Microscopy Dr. Robert Akins 1.5 hrs WS #12 Immunofluorescence for Kidney Biopsies Dr. Robert Garola 1.5 hrs 1:00 pm to 4:30 pm WS #13 Microwave Processing Connie Wavrin 3 hrs WS #14 Molecular Biology Abraham Joseph 3 hrs *Classes Subject to Change ^ CEU?s Pending Approval from NSH 1:00 pm to 2:30 pm WS #15 Round Table- to Discuss Options in Histology-Several Speakers will discuss other options for careers in histology, such as Pharmaceutical Industry, Veterinary & Research or Industry Options for Sales and Technical Training 1.5 hrs WS #16 ISH, FISH, PCR Mitch Gore 1.5 hrs WS #17 FNA Cytology Osman Ouattara 1.5 hrs 3:00 pm to 4:30 pm WS #18 Bog People of Northern Europe Sandra Olson PhD 1.5 hrs Saturday Seminars*^ 8:30 am to 12:00 pm WS #19 IHC-Locating and Validating New Antibodies and IHC Reagents Charlie Dorner 3 hrs WS #20 Ergonomics Jan Minchew 3 hrs 8:30 am to 10:00 am WS #21 Non-healing Lesion-Delusion or Parasite? David Brenner 1.5 hrs WS #22 Forensic & Medical Entomology Carolyn & Vincent D?Amico 1.5 hrs 10:30 am to 12:00 pm WS #23 Interesting Cases in Veterinary Pathology Dr. Perry Habecker 1.5 hrs WS #24 Clinical Flow Cytometry Brenda Rabeno 1.5 hrs 1:00 pm to 4:30 pm WS #25 IHC for Animal Histology (24 limit-wet) Zahra Naser 3 hrs WS #26 Safety for Histology Sylvia Casey 3 hrs WS #27 Grossing for Histology Connie Wavrin 3 hrs 1:00 pm to 2:30 pm WS #28 Mad Cow Disease & Other Transmissible Dr. Michael Kanzer 1.0 hrs Spongiform Encephalopathies 3:00 pm to 4:30 pm WS #29 Lab Math and Chemistry Donna Montegue 1.5 hrs 8:30 am to 4:30 pm WS #30 QIHC Preparation Ethel Macrea 6 hrs WS #31 HT Readiness Linda Foster-Brown 6 hrs * Classes subject to change ^ CEU?s Pending Approval from NSH Fees Students & Retirees** ? Day Session $ 40.00 ? Day Session $ 20.00 Full Day Session $ 80.00 Full Day Session $ 40.00 Meet and Greet- seminar attendees included, guests $25.00 Registration Fee $25.00 ?waived if registered by August 15th 10% off groups of four or more from same laboratory in attendance All workshops are subject to change **proper student ID must be mailed with registration ________________________________________________________________________ Full Program with abstracts will be available soon. If you would like to register now please fill in the following: Name ______________________________________ Address ____________________________________ ____________________________________ ____________________________________ Place of employment _________________________ Home # ________________ Work# _____________ E-mail _____________________________________ ________________________________________________________________________ Workshops planning to attend Thursday September 6th _________ _________ _________ Friday September 7th _________ _________ _________ _________ Saturday September 8th _________ _________ _________ _________ Number planning to attend Meet and Greet ________ Total amount enclosed ___________ ________________________________________________________________________ Please send completed forms with payment to: HSD PO Box 5341 Wilmington, DE 19808-0341 Any questions please contact: Michelle Hart at mhart@christianacare.org Elaine Perkins at eperkins@christianacare.org Kristen Broomall at histotechkb@gmail.com You may also contact your local histology societies for information concerning the 2007 Region II Fall Symposium being held at DTCC in Stanton, Delaware. Hotels in the area of DTCC Stanton Campus Courtyard by Marriot-48 Geoffrey Dr. 302-456-3800 ? Block of rooms being held at $114.00/single and $124.00/double until August 9th Christiana Hilton-100 Continental Dr. 302-454-1500 ? Block of rooms being held at $129.00/single and $149.00/double until August 6th Days Inn-900 Churchman?s Rd. 302-368-2400 Country Inn and Suites-1024 Old Churchman?s Rd. 302-266-6400 Fairfield Inn by Marriot-65 Geoffrey Dr. 302-292-1500 Red Roof Inns-415 Stanton-Christiana Rd. 302-292-2870 Homestead Studio Suites Hotel-333 Continental Dr. 302-283-0800 Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From judi.ford <@t> roche.com Thu Aug 9 10:09:46 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Thu Aug 9 10:10:05 2007 Subject: [Histonet] Masson's Troubleshooting Message-ID: Good morning to all from not so sunny northern CA: I want to thank you for all the input you gave me about my problem with the trichrome, especially to John, Gayle, Gundrun and Sarah. I re-examined the slides and compared the control with a Gomori's One-Step control slide, which had be stained a couple days prior. Both stains used the same control tissue. When I first looked at the Gomori's slide the collagen appeared to be stained well with the green. The same area in the Masson's didn't stain at all with the aniline blue. Upon examining both slides this morning I noticed that even though the green stain showed there was still some red stain within it. So, we are ordering new phosphotungstic acid and I'm cutting new slides. My co-worker is staining Masson's this morning with some other control slides and I'll try again with the new slides. If we're having the same results then I'm assuming that its got to be the phosphotungstic acid and we'll try again when the new stuff arrives. Anyway, I really appreciate all of your suggestion and input. This board has always been a valuable resource for me. Cheers! Judi Ford Palo Alto, CA From TJJ <@t> Stowers-Institute.org Thu Aug 9 10:22:58 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Aug 9 10:23:38 2007 Subject: [Histonet] Job Posting - Research Histology, Kansas City, MO Message-ID: The Stowers Institute for Medical Research has an opening for a Histology Specialist II to provide high quality research histology services. Responsibilities include sample preparation including sample receipt, fixation, processing, embedding, microtomy and staining; operation and maintenance of histology prep and ancillary equipment; training researchers in the use of common equipment and preparation techniques; and development of protocols for specialized research histology-related projects. In addition to the ability to effectively function in a team-oriented environment, the successful candidate must have fine-motor skills to manipulate tiny tissue samples and to demonstrate histology techniques; excellent communication skills to interact with the research scientists; a familiarity with basic biology, cell biology or genetics; and a record of excellent general laboratory skills. Minimum requirements include an Associates Degree in Biology or a related field and two years experience in basic research using histology techniques. Preference will be given to individuals with a Bachelors Degree in a related field, and or additional experience in basic research histology. For benefits information and to apply: http://www.stowers-institute.org/ScientistsSought/ScientistsSought.asp#r esume Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From histocontract <@t> gmail.com Thu Aug 9 10:58:31 2007 From: histocontract <@t> gmail.com (Human Resources) Date: Thu Aug 9 10:58:43 2007 Subject: [Histonet] histology contractor needed In-Reply-To: References: Message-ID: Temporary Histology Contractor needed ? 4-6 months We are a well-known biotechnology company located in Silicon Valley, CA. We are looking for an experienced histotechnician to help out in our histology lab in a dynamic research setting. The successful candidate should have experience with basic histology procedures such as tissue trimming, processing, paraffin embedding, cutting, and staining with H&E, as well as experience with common special stains, and a facility with cryosectioning. Qualified candidates must have a minimum of 3-5 years relevant experience. Certification in HT and in phlebotomy would be a plus. To apply, please send resume to *histocontract@gmail.com* *,* along with desired hourly rate of pay. From Heather.D.Renko <@t> osfhealthcare.org Thu Aug 9 11:08:21 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Aug 9 11:08:49 2007 Subject: [Histonet] NSH Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0777558A@pmc-rfd-mx01.intranet.osfnet.org> I received my packet in June and registered. Check your membership expiration possibly? Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From wilson_c <@t> ricerca.com Thu Aug 9 11:23:24 2007 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Thu Aug 9 11:23:39 2007 Subject: [Histonet] Blocking arrangements in research for different species Message-ID: <9D443EB9D0270143B5AAF190CB1A58A305064EB2@dogwood.ricerca.com> I was wondering if anyone out there might be willing to share their blocking arrangement for different species in research with me via email or fax. Or a specific resource that I could find online, book, etc.. I've googled without much success so far. I need somewhere to start and any and all help would be appreciated. Thanks, Carol Carol Wilson Team Leader - Histology Ricerca Biosciences, LLC 7528 Auburn Rd. Concord, OH 44077 440-357-3930 From jnocito <@t> satx.rr.com Thu Aug 9 11:51:02 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 9 11:51:20 2007 Subject: [Histonet] Slide Staining Variability References: <661949901A768E4F9CC16D8AF8F2838CA1C6AE@IS-E2K3.grhs.net> Message-ID: <003d01c7daa5$7a4f8800$d49eae18@yourxhtr8hvc4p> we have had the same problem on all three machines. To date, it has been: technician error, failure to remove a yellow locking ring, vortex mixer, slide warmer not hot enough, slide warmer too hot, dispenser not working, Venus is not aligned with Mars. Most of the time we have had to get a service rep out to repair something or other. One machine has had the carousel replaced twice. Need I say more? Oh yeah, I know you're here, go call my employer like you did last time. Oh, I should say "former" employer. Once again, the views of this editorial are my own and I have not been coerced, drugged, or beaten with a feather. JTT ----- Original Message ----- From: "Mike Pence" To: "Annette Hall" ; Sent: Thursday, August 09, 2007 9:59 AM Subject: RE: [Histonet] Slide Staining Variability We have had some of these same problems and it is always been a nozzle or mixer problem. Do you perform regular PM's? The reaction buffer ph could also be off, that can cause varied staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Hall Sent: Thursday, August 09, 2007 8:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Staining Variability Histonetters, For those of you who use automated IHC staining systems, has anyone had issues with part of the slide staining and the rest of the slide not? We have adopted the policy of placing the control on with the patient tissue as a more comprehensive check of the system. However, we occasionally find the control is acceptable but the patient fails to even counterstain. This has been an intermittent problem on the Ventana Benchmark for some time, but the frequency has been increasing to almost daily. Any thoughts? Thanks, Annette J Hall Histo/Cyto/Micro Supervisor United Clinical Laboratories Dubuque, IA 52001 -----Original Message----- From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] Sent: Monday, August 06, 2007 3:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer w/integrated coverslipper Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dshtilki <@t> uci.edu Thu Aug 9 12:21:45 2007 From: dshtilki <@t> uci.edu (Shtilkind, Dmitriy) Date: Thu Aug 9 12:21:57 2007 Subject: [Histonet] Masson's Trichrome Troubleshooting Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03057C20@GUS.hs.uci.edu> Acetic acid is just fixative. It does not remove the staining. You need to make sure that all slides went thru fresh Bouin's first. Controls might have been fixed in Bouin's but tissues haven't. So control will work but tissues will not. Dmitriy From Wanda.Smith <@t> HCAhealthcare.com Thu Aug 9 13:26:42 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Thu Aug 9 13:27:59 2007 Subject: [Histonet] A Laboratory Microwave-NOT for Processing Message-ID: <817C2761C5A1394180709AEEDB775B7E03193FFE@NASEV03.hca.corpad.net> Dear Netters & Vendors, It is capital equipment budget time for 2008 and I need to replace my "household" microwave that I use for special stains and other misc things. I do not process tissue in the microwave and I don't anticipate doing so in the near future. What basic laboratory grade microwaves are available and can I get some quotes? Thanks, Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com From godsgalnow <@t> aol.com Thu Aug 9 13:37:02 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Aug 9 13:37:17 2007 Subject: [Histonet] Anyone still doing cytospins? Message-ID: <8C9A8CB69C8C867-B6C-2820@webmail-dd14.sysops.aol.com> We recently switched to ThinPrep and we have about 1200 Sacamanno Physicians Collection Kits as well as 5000 filter cards for the cyto spins and 2 cytospin centrofuges that hold 4 slides and 2 thermo centrofuges that hold 4 of the 50ml tubes. We would sell it to you for a good(cheap) price plus shipping......... Roxanne Soto HT(ASCP)QIHC Lab Manager Tampa, Florida ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From NMargaryan <@t> childrensmemorial.org Thu Aug 9 13:43:44 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Aug 9 13:42:51 2007 Subject: [Histonet] RE: Slide Staining Variability In-Reply-To: References: Message-ID: Hi Annette and histonetters, I am having same problem constantly on the automated IHC staining systems from LabVision. I do cleaning of machine after 2-3 running independently on number of slides. I clean machine, but do my staining manually; I can't waist tissue and reagents.............. I do call company; they are very nice people, came several times, check, but problem still there. It is so frustrating:-(:-(:-( Thanks Naira ______________________________________________________ Message: 10 Date: Thu, 9 Aug 2007 08:41:50 -0500 From: Annette Hall Subject: [Histonet] Slide Staining Variability To: Histonet@lists.utsouthwestern.edu Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B6054@mercury.pa-ucl.com> Content-Type: text/plain Histonetters, For those of you who use automated IHC staining systems, has anyone had issues with part of the slide staining and the rest of the slide not? We have adopted the policy of placing the control on with the patient tissue as a more comprehensive check of the system. However, we occasionally find the control is acceptable but the patient fails to even counterstain. This has been an intermittent problem on the Ventana Benchmark for some time, but the frequency has been increasing to almost daily. Any thoughts? Thanks, Annette J Hall Histo/Cyto/Micro Supervisor United Clinical Laboratories Dubuque, IA 52001 -----Original Message----- From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] Sent: Monday, August 06, 2007 3:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer w/integrated coverslipper Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy _______________________________________________ From sjchtascp <@t> yahoo.com Thu Aug 9 13:52:18 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Aug 9 13:52:29 2007 Subject: [Histonet] H&E special stains Quality assurance Message-ID: <471688.98892.qm@web38208.mail.mud.yahoo.com> I'm working as an HT temp at a histology lab with no ASCP HT's. They need a basic H&E and special stains QC sheet to hand in with there slides to the pathologist. If someone has a good but simple one they do not mind sharing I'm sure it would be well recieved. Thanks, Attention Steve Derm Path Fax 608-263-3020 --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. From godsgalnow <@t> aol.com Thu Aug 9 14:04:09 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Aug 9 14:04:44 2007 Subject: [Histonet] Anyone still doing cytospins? In-Reply-To: <8C9A8CB69C8C867-B6C-2820@webmail-dd14.sysops.aol.com> References: <8C9A8CB69C8C867-B6C-2820@webmail-dd14.sysops.aol.com> Message-ID: <8C9A8CF338A196F-B6C-29A3@webmail-dd14.sysops.aol.com> I also have a Cytospin 4 from Thermo that holds 12 slides.? It was purchased brand new and we used it for 2 months and then we switched to ThinPrep--I forgot about it. Roxanne -----Original Message----- From: godsgalnow@aol.com To: histonet@lists.utsouthwestern.edu Sent: Thu, 9 Aug 2007 2:37 pm Subject: [Histonet] Anyone still doing cytospins? We recently switched to ThinPrep and we have about 1200 Sacamanno Physicians Collection Kits as well as 5000 filter cards for the cyto spins and 2 cytospin centrofuges that hold 4 slides and 2 thermo centrofuges that hold 4 of the 50ml tubes. We would sell it to you for a good(cheap) price plus shipping......... Roxanne Soto HT(ASCP)QIHC Lab Manager Tampa, Florida ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From godsgalnow <@t> aol.com Thu Aug 9 16:07:59 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Aug 9 16:08:13 2007 Subject: [Histonet] used equipment Message-ID: <8C9A8E08029064C-B6C-311E@webmail-dd14.sysops.aol.com> I have used equipment and some peripherals for sale.? You pay shipping. Olympus Microtome??????????????????????????????????????????????????????????????????? $2000 10 cases of 20 packs(25 in each pack)?of 15 ml conical tubes??????????? $600 2 Thermo, 4-tube 50 ml centrifuges?????????????????????????????????????????????? $1000 each 2 ATP-1 Tissue Processors???????????????????????????????????????????????????????????$6000 each Roxanne Soto HT(ASCP)QIHC Lab Manager?? ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From HornHV <@t> archildrens.org Thu Aug 9 17:14:03 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 9 17:14:29 2007 Subject: [Histonet] htl's Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB72A8@EMAIL.archildrens.org> Is a person with an HTL certification considered 'high complexity testing personnel' even if they do not have a BS degree? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From mtarango <@t> nvcancer.org Thu Aug 9 19:17:53 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Aug 9 19:18:12 2007 Subject: [Histonet] Slide Staining Variability In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C012B6054@mercury.pa-ucl.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6DC5@NVCIEXCH02.NVCI.org> I have had this problem before, using the same immunostainer that you're using. The problem was the slides, believe it or not. The slides we were using before were too hydrophobic, aqueous-based reagents would bead up and often run right off the slide, into the waste. The slides would work great at keeping the tissue on, but you have to make sure that the reagent doesn't run off the slide. There isn't an automated stainer that I've seen that can check this. You might want to try different slides and see if it makes a difference. I could tell you which slides weren't working for me, but I don't want to get myself into trouble. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Hall Sent: Thursday, August 09, 2007 6:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Staining Variability Histonetters, For those of you who use automated IHC staining systems, has anyone had issues with part of the slide staining and the rest of the slide not? We have adopted the policy of placing the control on with the patient tissue as a more comprehensive check of the system. However, we occasionally find the control is acceptable but the patient fails to even counterstain. This has been an intermittent problem on the Ventana Benchmark for some time, but the frequency has been increasing to almost daily. Any thoughts? Thanks, Annette J Hall Histo/Cyto/Micro Supervisor United Clinical Laboratories Dubuque, IA 52001 -----Original Message----- From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] Sent: Monday, August 06, 2007 3:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer w/integrated coverslipper Histonetters, Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) with the integrated coverslipper (CV5030)? Also, can you tell me if you've experienced any trouble with it and how long you've been using it? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From tkngflght <@t> yahoo.com Thu Aug 9 22:42:30 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Aug 9 22:42:31 2007 Subject: [Histonet] Dehydrating beads In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635084@hpes1.HealthPartners.int> Message-ID: <001201c7f354$4f026d30$6501a8c0@CHERYLSLAPTOP> Hey-- I used these in a lab where I temped a few weeks ago and I LOVE THEM!! I've been in a lot of labs and used a lot of different stainers and this was a big X-Y Leica with the upright slide racks. When staining the xylene is stirred up with the movement of the slides and the beads enabled us to handle less xylene (exposure from rotating) and the slides when slipped appeared to clear under the coverslips both faster and more thoroughly. To dry them you lay them near your air cleaner as you leave for the day so you don't breathe it, and you just need enough to rotate them through each day. My two cents! Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.883.7704 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, August 08, 2007 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dehydrating beads I know this was discussed in the recent past, but, what are the pros and cons of using these and how much do they save on xylene usage? Also, how does one get into the archives of Histonet to view past responses if those of you who already answered do not want to post an answer agin (and I do understand not rehashing old subjects!!) I cannot say enough how VERY much I appreciate the use of this resource for problem solving in the histology world!! The networking is invaluable and I want to thank everyone out there for the quick responses and helpful information on my last post concerning automated microtomes!!!!!! Histotechs are a truly a CUT above the rest!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Aug 9 23:04:37 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Aug 9 23:04:43 2007 Subject: [Histonet] Slide Staining Variability Message-ID: Into trouble with whom? The suppliers of hydrophobic (= greasy) slides that won't work in an automated immunostaining system? Non-wettable (is that a word?) glass slides have no place in biological microtechnique. Many years ago, the cheapest slides on the market had to be washed with detergent and carefully rinsed and dried (distilled water, acetone etc) before they would accept frozen, paraffin or semithin plastic sections. This was an occasional annoyance in the 1960s. Since the late 1960s cheap slides have been clean: free of grit and grease. That's my experience. Are there vendors of still-available greasy, dirty, gritty microscope slides? Who buys them? John Kiernan London, Canada ------------------ ----- Original Message ----- From: "Tarango, Mark" Date: Thursday, August 9, 2007 20:19 Subject: RE: [Histonet] Slide Staining Variability To: Annette Hall , Histonet@lists.utsouthwestern.edu > I have had this problem before, using the same immunostainer > that you're > using. The problem was the slides, believe it or > not. The slides we > were using before were too hydrophobic, aqueous-based reagents would > bead up and often run right off the slide, into the waste. > > The slides would work great at keeping the tissue on, but you > have to > make sure that the reagent doesn't run off the slide. > There isn't an > automated stainer that I've seen that can check this. You > might want to > try different slides and see if it makes a difference. > > I could tell you which slides weren't working for me, but I > don't want > to get myself into trouble. > > > > Mark Adam Tarango HT(ASCP) > > Histology/Immunohistochemistry Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > mtarango@nvcancer.org > > Direct Line (702) 822-5112 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > AnnetteHall > Sent: Thursday, August 09, 2007 6:42 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide Staining Variability > > Histonetters, > > For those of you who use automated IHC staining systems, has > anyone had > issues with part of the slide staining and the rest of the slide > not? We > have adopted the policy of placing the control on with the patient > tissue as > a more comprehensive check of the system. However, we > occasionally find > the > control is acceptable but the patient fails to even > counterstain. This > has > been an intermittent problem on the Ventana Benchmark for some > time, but > the > frequency has been increasing to almost daily. > > Any thoughts? > > Thanks, > Annette J Hall > Histo/Cyto/Micro Supervisor > United Clinical Laboratories > Dubuque, IA 52001 > > -----Original Message----- > From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] > Sent: Monday, August 06, 2007 3:35 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica autostainer w/integrated coverslipper > > Histonetters, > Could anyone tell me if they're using the Leica AutoStainer XL > (ST5010)with the integrated coverslipper (CV5030)? > Also, can you tell me if you've experienced any trouble with it > and how > long you've been using it? > > Thanks, > Sandy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please > notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "EMF " made the following annotations. > ----------------------------------------------------------------- > ------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended > recipient(s) and may contain confidential, proprietary, > and/or privileged information protected by law. If you are > not the intended recipient, you may not use, copy, or > distribute this e-mail message or its attachments. If you > believe you have received this e-mail message in error, > please contact the sender by reply e-mail and destroy all > copies of the original message > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Thu Aug 9 23:04:50 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Aug 9 23:04:53 2007 Subject: [Histonet] Slide Staining Variability Message-ID: From melissa.mazan <@t> tufts.edu Fri Aug 10 07:26:35 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Fri Aug 10 07:26:52 2007 Subject: [Histonet] RE: post FACS staining In-Reply-To: <200708091713.l79HDE9I021348@mail-proofpoint-2a.usg.tufts.edu> References: <200708091713.l79HDE9I021348@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <46BC597B.5080700@tufts.edu> Hi all, Thanks so much for your advice. On your questions: We sort a whole lung cell suspension based on CD45, CD31 negative, sca-1 positive. I have hoped that as these are cell surface markers and I"m staining for cytoplasmic antigens, I would be able to distinguish between leftover Ab (which should only be the sca-pos, as the others are a negative sorting strategy) and the proteins I"m looking for. If I look at the cytos before any further staining, I often see a fluorescent rim around the cell, which is what sca-1 should look like. The antibodies that I am using work very well on mouse tissues and on Western blot. For pro SP-C, in particular, my positive control (ATII cells) fluoresce very brightly and are reminiscent of tissue cells - punctate color throughout the cytoplasm. What is confusing is that my negative control (serum only) does not fluoresce beyond the faint rim of stain from leftover sca-1 staining, (all well and good) but my C45neg/CD31neg/Sca-1 pos cells have a weak fluorescence that is distinctly different to neg control, but nowhere near pos control. When I stain with CC10, I have a similar result, although I do not have a good pos control other than tissue (no post FACS pos control). It would be nice if this were real, but when I stain the CD45neg/CD31neg/Sca-1neg fraction, I get the same result -which makes no sense. I wonder if the FACS is changing, for instance, the charge on the cells sufficiently, or is perhaps changing the permeability of the cell sufficiently to cause this sort of non-specific staining? I have tried methanol/acetic acid - didn't like what it did to the morphology of the cells; acetone alone; acetone and cytofix/cytoperm, and cytofix/cytoperm alone. I agree with the increased background with the BSA - however, its difficult to keep good morphology of the cells without. I'm coming to the conclusion that post-FACS immuncytochemistry may be a bad idea...Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Histonet Digest, Vol 45, Issue 12 (ryan@upei.ca) > 2. RE: immunostaining post-FACS (Tarango, Mark) > 3. Re: Masson's Trichrome Troubleshooting (John Kiernan) > 4. Re: Golgi stainembedding (John Kiernan) > 5. change in email address (Dawn Cowie) > 6. Re: change in email address (LINDA MARGRAF) > 7. RE: NSH catalog (Bartlett, Jeanine (CDC/CCID/NCZVED)) > 8. NSH Symposium/Convention (Aubrey Wanner) > 9. Re: change in email address (Joe Nocito) > 10. Slide Staining Variability (Annette Hall) > 11. (no subject) (Bruijntjes, J.P. (Joost)) > 12. Slide Staining Variability (Judy Collins) > 13. Ethanol, never methanol for CD marker fixation RE: [Histonet] > immunostaining post-FACS (Gayle Callis) > 14. RE: Slide Staining Variability (Mike Pence) > 15. Region II Fall Seminar for September 6, 7 and 8, 2207 > (Pamela Marcum) > 16. Masson's Troubleshooting (Ford, Judi) > 17. Job Posting - Research Histology, Kansas City, MO (Johnson, Teri) > 18. histology contractor needed (Human Resources) > 19. NSH (Renko, Heather D.) > 20. Blocking arrangements in research for different species > (Wilson, Carol) > 21. Re: Slide Staining Variability (Joe Nocito) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 8 Aug 2007 20:33:05 -0300 > From: > Subject: [Histonet] Histonet Digest, Vol 45, Issue 12 > To: histonet@lists.utsouthwestern.edu > Message-ID: <8C0B2B7720B@acad1.cs.upei.ca> > > C.L. Ryan will be out of the office from Friday Aug 10 until Monday Aug. 13, incl. > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 8 Aug 2007 17:08:08 -0700 > From: "Tarango, Mark" > Subject: RE: [Histonet] immunostaining post-FACS > To: "Melissa Mazan" , > histonet@lists.utsouthwestern.edu > Message-ID: > <5AEC610C1CE02945BD63A395BA763EDE011B6DB8@NVCIEXCH02.NVCI.org> > Content-Type: text/plain; charset=us-ascii > > These cells haven't already been stained and sorted or something, have > they? If so, I would assume that whatever antibodies they used for > flow/FACS will be on the cells. You said you use cytofix/cytoperm, that > might denature the flow antibodies (if there are any), but you never > know. > > Have you tried doing the cytofix/cytoperm on the cells while they are in > suspension, and THEN making the cytospins? Switching to a different > fixative? Gayle Callis had a post a while ago about using a fixative of > 75% acetone and 25% methanol for murine CD markers. Maybe it would work > for all your markers. I've tried modifying this fixative using propanol > instead of ethanol for frozen sections on human tissue. It worked. > > When all else fails, I like to, as closely as possible, treat the > specimen like formalin-fixed paraffin-embedded tissue. I will fix in > formalin, dehydrate in staining dishes (like processing tissue) ...then > bring it back to water and do antigen retrieval before staining. You > might lose too many cells trying this on cytospins though, but might as > well throw it out there. > > > Just some thoughts... > > > Mark Adam Tarango HT(ASCP) > > Histology/Immunohistochemistry Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > mtarango@nvcancer.org > > Direct Line (702) 822-5112 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa > Mazan > Sent: Wednesday, August 08, 2007 4:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] immunostaining post-FACS > > Hi all, Wondering if anyone has done much immunostaining post FACS. WE > have had a lot of trouble with this procedure - we collect our cells > (from murine lung) into BSA on ice, bring them back to our lab (FACS > is at our core facility, so it means about an hour and a half before > the cells get to our lab). In the lab, we immediately cytospin - the > morphology is nice on H and E - and then fix in Cytofix Cytoperm for 20 > minutes at 4C. We either commence immunostaining immediately after, or > leave in fridge for the next day. We do either immunofluorescence or > immunoenzyme (usually ABC system) for identifying antigens of interest. > We always have a negative control, but don't always have a positive > control. We do either double staining for SPC and CC10 or single > staining for alpha sma, vimentin, e-cad, or pancytokeratin. The > problem that we have is that although the negative controls are very > negative with respect to the cases, we seem to be getting a lot of > false positives when the antibody is actually applied - for instance, > my cells will stain 80% positive - strongly positive - using alpha sma, > but a Western of the same cells will show no alpha-sma at all, whereas > there is positive staining on whole lung suspensions. Any ideas? > Advice for getting good post-FACS immunostaining? Many thanks - Melissa > > Melissa R. Mazan, DVM, Diplomate ACVIM > Associate Professor and Director of Equine Sports Medicine > Department of Clinical Sciences > Tufts Cumming School of Veterinary Medicine > 200 Westborough Road > North Grafton, MA 01536 > Tel:508-839-5395 > Fax:508-839-7922 > email: melissa.mazan@tufts.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "EMF " made the following annotations. > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended > recipient(s) and may contain confidential, proprietary, > and/or privileged information protected by law. If you are > not the intended recipient, you may not use, copy, or > distribute this e-mail message or its attachments. If you > believe you have received this e-mail message in error, > please contact the sender by reply e-mail and destroy all > copies of the original message > ============================================================================== > > > > > ------------------------------ > > Message: 3 > Date: Thu, 09 Aug 2007 00:01:04 -0400 > From: John Kiernan > Subject: Re: [Histonet] Masson's Trichrome Troubleshooting > To: "Ford, Judi" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > If the known positive control slides were side-by side with the study slides (which stained correctly), then there must be something wrong with the control slides, not the solutions or the steps of the method. Different fixation or inadequate removal of paraffin come to mind, but you've probably thought of those. Your study slides of heart etc were well stained, so perhaps this will be a suitable positive control for future Masson stainings. > > It would be satisfying, however, to know why previously positive collagen in your sections of muscle etc failed to stain with aniline blue. Was the collagen in these sections red or not stained at all? If it was red, then the PTA/PMA had failed to displace the red dye, and a longer time in PTA/PMA is likely to be needed. If that's the case the control sections are unsuitable for staining beside your study sections. The final wash is in dilute acetic acid to prevent the removal of any bound red or blue dye, which can occur if plain water is used. You rightly examined the wet sections at this stage, an action that shows that you know what you are about, unlike many professors, postdocs, graduate students, . . . > > Please let us all know if you find out why the collagen in previously good positive-control tissue became unstainable with the Masson's method in Luna's AFIP manual. > > John A. Kiernan > Department of Anatomy & Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > http://publish.uwo.ca/~jkiernan/index.htm > --- > ----- Original Message ----- > From: "Ford, Judi" > Date: Wednesday, August 8, 2007 15:48 > Subject: [Histonet] Masson's Trichrome Troubleshooting > To: histonet@lists.utsouthwestern.edu > > >> Hi everyone, >> >> >> >> I'm hoping someone can give me suggestions on what happened to >> my stain. >> I did a Masson's Trichrome on mouse hearts/aortas this >> morning. I >> followed the AFIP protocol exactly and used two different >> control slides >> (tongue, aorta, skeletal muscle). We have previously stained control >> slides with the tissue I used this time and they worked beautifully. >> >> >> >> After finishing the glacial acetic acid rinse I examined the slides >> under the scope and found that neither of the control slides stained >> with the aniline blue, but all the study slides stained >> beautifully with >> aniline blue. I tried going back and restaining (from >> phosphomolybdic/phosphotungstic acid) the slides and the same results >> showed. I thought maybe I had them in the glacial acetic acid >> too long >> so I cut that back to 3 minutes instead of 5 for the second round. >> >> >> >> Any ideas on what could have happened? Could the control slides have >> been sitting too long as unstained slides? Could the aniline blue >> working solution need an extra punch from glacial acetic acid? >> In the >> past I've reused aniline blue without any problems and this solution >> wasn't past its expiration date. >> >> >> >> Would love to hear your thoughts...... >> >> >> >> Judi Ford >> >> Palo Alto, CA >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 4 > Date: Thu, 09 Aug 2007 01:00:14 -0400 > From: John Kiernan > Subject: Re: [Histonet] Golgi stainembedding > To: Bob Nienhuis > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Dear Bob N. > > If your archived tissue is in formaldehyde you probably will not be able to get Golgi preparations that are good enough for research purposes. > > There are plenty of published Golgi techniques for ideally fixed material (up to a few weeks). > > Researchers demanding perfect dendritic morphology (for counting dendritic spines, or making mathematical models of branching) often fix their specimens in Cox's solution and apply the alkaline developer to thick sections cut with a vibrating microtome (Vibratome or similar). This can provide superb Golgi preparations, even with the brains of adult animals. Kolb, at Lethbridge University, is a major proponent of these methods. His publications should be available in your library. Try Scopus or Web of Science. > > For good Golgi preparations you need to work closely with someone experienced with the various techniques and you must become familiar with the technical literature. Two books have chapters that serve as introductory reading: > Bradley, PB (ed) 1975. Methods in Brain Research . Wiley. > Santini, M. 1975. Golgi Centennial Symposium. Raven Press. > > There are two families of Golgi methods. The intracellular chromate ions may be precipitated by silver or mercury, and the results are not the same. I wish you well with obtaining good Golgi preps on fixed human brains. If you perfect the technique you will have the makings of a significant publication. > > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada. > --- > ----- Original Message ----- > From: Bob Nienhuis > Date: Wednesday, August 8, 2007 14:59 > Subject: [Histonet] Golgi stainembedding > To: histonet@lists.utsouthwestern.edu > > >> Anybody have suggestions for alternatives to celloidin embedding >> for Golgi-Kopsch? Has anyone done it, and can point out some >> pitfalls I might avoid in doing it? I want to Golgi stain some >> archival human brain tissue. >> >> I have not tried it yet, and understand it can be a bit tricky. >> >> Bob >> nienhuis@ucla.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 5 > Date: Thu, 9 Aug 2007 05:35:20 -0700 (PDT) > From: Dawn Cowie > Subject: [Histonet] change in email address > To: histonet > Message-ID: <771388.95993.qm@web81007.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Histonet, > please change my email address for receiving messages to Dawn_Cowie@yahoo.com > > thanks, > Dawn > > > ------------------------------ > > Message: 6 > Date: Thu, 09 Aug 2007 08:02:05 -0500 > From: "LINDA MARGRAF" > Subject: Re: [Histonet] change in email address > To: "histonet" , "Dawn Cowie" > > Message-ID: <46BAC9FD020000DA000113A0@CNET3.CHILDRENS.COM> > Content-Type: text/plain; charset=US-ASCII > > Dear Dawn (and other Histonetters): > I will change your address on the Histonet email address list as you requested but I wanted to point out to you and all the other Histonet members that you can change your own address or change other features of your subscription by going to the website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and using the instructions at the bottom of the page. People can switch to digest mode, temporarily stop messages while they go on vacation etc. If you cannot remember your password you can request it there too. For anyone having trouble posting messages because the server says you are not a member, this likely means your address has changed somewhat from when you subscribed (a common occurrence in institutional email systems) and you need to update your address which you can do there. > By the way Histonet is up tp >2600 members now! > Thanks, > Linda M > Histonet administrator > > >>>> Dawn Cowie 08/09/07 7:35 AM >>> >>>> > Dear Histonet, > please change my email address for receiving messages to Dawn_Cowie@yahoo.com > > thanks, > Dawn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Thu, 9 Aug 2007 09:12:29 -0400 > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > Subject: RE: [Histonet] NSH catalog > To: "Kim Tournear" , "Histonet" > > Message-ID: > <34BB307EFC9A65429BBB49E330675F7202BDBEF3@LTA3VS003.ees.hhs.gov> > Content-Type: text/plain; charset=us-ascii > > I received mine a few weeks ago. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Tournear > Sent: Wednesday, August 08, 2007 7:13 PM > To: Histonet > Subject: [Histonet] NSH catalog > > Am I the only one that hasn't received the NSH catalog for the symposium > in Denver, or do we have to get it on line now? > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Moody friends. Drama queens. Your life? Nope! - their life, your story. > Play Sims Stories at Yahoo! Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 8 > Date: Thu, 9 Aug 2007 09:26:15 -0400 > From: "Aubrey Wanner" > Subject: [Histonet] NSH Symposium/Convention > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > The 33rd Annual NSH Symposium/Convention will take place in Denver, > October 26-31. Registration programs mailed in May - if you did not > receive a copy please feel free to contact the NSH Office, 443-535-4060 > or visit the website and click on Meetings/Events > > Symposium/Convention. > > All workshops are still available and we still have rooms in the > headquarters hotel. Any questions please call our office - we hope to > see you there! > > > > Mrs. Aubrey M.J. Wanner > Meeting Manager, Annual Symposium/Convention > Managing Editor, Journal of Histotechnology > > > We've Moved! > National Society for Histotechnology > 10320 Little Patuxent Parkway | Suite 804 > Columbia, MD 21044 > P | 443.535.4060 > Direct | 443.535.4065 > F | 443-535-4055 > http://www.nsh.org/ > > > > > ------------------------------ > > Message: 9 > Date: Thu, 9 Aug 2007 08:40:05 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] change in email address > To: "LINDA MARGRAF" , "histonet" > , "Dawn Cowie" > > Message-ID: <000b01c7da8a$ccd435f0$d49eae18@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > WOW! I think it's time again to thank Dr. M and her staff for maintaining > the Histonet. Party in Denver!? > > ----- Original Message ----- > From: "LINDA MARGRAF" > To: "histonet" ; "Dawn Cowie" > > Sent: Thursday, August 09, 2007 8:02 AM > Subject: Re: [Histonet] change in email address > > > Dear Dawn (and other Histonetters): > I will change your address on the Histonet email address list as you > requested but I wanted to point out to you and all the other Histonet > members that you can change your own address or change other features of > your subscription by going to the website > http://lists.utsouthwestern.edu/mailman/listinfo/histonet and using the > instructions at the bottom of the page. People can switch to digest mode, > temporarily stop messages while they go on vacation etc. If you cannot > remember your password you can request it there too. For anyone having > trouble posting messages because the server says you are not a member, this > likely means your address has changed somewhat from when you subscribed (a > common occurrence in institutional email systems) and you need to update > your address which you can do there. > By the way Histonet is up tp >2600 members now! > Thanks, > Linda M > Histonet administrator > > >>>> Dawn Cowie 08/09/07 7:35 AM >>> >>>> > Dear Histonet, > please change my email address for receiving messages to > Dawn_Cowie@yahoo.com > > thanks, > Dawn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Thu, 9 Aug 2007 08:41:50 -0500 > From: Annette Hall > Subject: [Histonet] Slide Staining Variability > To: Histonet@lists.utsouthwestern.edu > Message-ID: > <9FC023A4AB52BB4D87DC6456081A822C012B6054@mercury.pa-ucl.com> > Content-Type: text/plain > > Histonetters, > > For those of you who use automated IHC staining systems, has anyone had > issues with part of the slide staining and the rest of the slide not? We > have adopted the policy of placing the control on with the patient tissue as > a more comprehensive check of the system. However, we occasionally find the > control is acceptable but the patient fails to even counterstain. This has > been an intermittent problem on the Ventana Benchmark for some time, but the > frequency has been increasing to almost daily. > > Any thoughts? > > Thanks, > Annette J Hall > Histo/Cyto/Micro Supervisor > United Clinical Laboratories > Dubuque, IA 52001 > > -----Original Message----- > From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] > Sent: Monday, August 06, 2007 3:35 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica autostainer w/integrated coverslipper > > Histonetters, > Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) > with the integrated coverslipper (CV5030)? > Also, can you tell me if you've experienced any trouble with it and how > long you've been using it? > > Thanks, > Sandy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > ------------------------------ > > Message: 11 > Date: Thu, 9 Aug 2007 16:34:53 +0200 > From: "Bruijntjes, J.P. (Joost)" > Subject: [Histonet] (no subject) > To: > Message-ID: <8865601DD17A554CB489C17FFD8A51B248507E@MAIL04.tsn.tno.nl> > Content-Type: text/plain; charset="us-ascii" > > Please, change my email address into joost.bruijntjes@tno.nl > > > > Thanks > > > > TNO.NL > > Joost Bruijntjes > > T +31 30 694 44 80 > F +31 30 694 49 86 > E joost.bruijntjes@tno.nl > > Disclaimer > > > > > This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html > > ------------------------------ > > Message: 12 > Date: Thu, 9 Aug 2007 10:35:05 -0400 > From: "Judy Collins" > Subject: [Histonet] Slide Staining Variability > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > With the Ventana system, staining variability on different parts of the > slide usually is a result of a problem with the vortex mixers, although > other issues such as dispensers not working properly can cause this as > well. You can run a test on the vortex mixers (Find the procedure in > the online manual). You can also call their technical support line for > assistance. > > Judy Collins > > > > ------------------------------ > > Message: 13 > Date: Thu, 09 Aug 2007 08:50:49 -0600 > From: Gayle Callis > Subject: Ethanol, never methanol for CD marker fixation RE: [Histonet] > immunostaining post-FACS > To: "Tarango, Mark" , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070809082551.01b37dd8@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Dear Mark and Melissa,' > > A correction to Marks reply. I do NOT use methanol in my acetone/alcohol > fixative. The fixative is made up with absolute ethanol 75% acetone/25% > absolute ETHANOL. Methanol ruins my CD markers (in the literature and also > in Histonet archives) . However, this acetone alcohol fixative is not good > with human CD4 and CD8 markers since they do not like ethanol either. And > there are antibodies that will work best with formalin or paraformaldehyde > fixation. > > Could it be the antigens you are trying to stain do not like the fixative > you are using? I suggest you do a fixation panel on known positive cells > to optimize the fixation. That positive control is important to know IF > your antibodies, and method is working. A known positive tissue control > may help too, then you know your antibodies are working on mouse tissues, > cells. Frozen sections and different fixatives should provide some > answers. Some antibodies work better on Western blots, but when you try to > use them on tissue sections (or at the same concentration as the blot) you > get nothing. Also, we try to purchase antibodies known to work on > cells/tissue sections, and avoid just the Western Blot application. > > Double check your BSA, and make sure it is protease/immunoglobulin free, > Jackson has this - there is an interesting publication on albumin causing > background staining in AIMM journal, titled Albumin in > Immuhohistochemistry: Foe or Friend? 14(4l ), December 2006 Mittelbronn M > et al. I do have the publication in pdf form if you would like to read it. > > I am curious and as Mark pointed out - to sort the cells, are you staining > those with an antibody, sort, collect, then stain with another > antibody? I was a bit lost on this. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > > > > > > At 06:08 PM 8/8/2007, you wrote: > >> These cells haven't already been stained and sorted or something, have >> they? If so, I would assume that whatever antibodies they used for >> flow/FACS will be on the cells. You said you use cytofix/cytoperm, that >> might denature the flow antibodies (if there are any), but you never >> know. >> >> Have you tried doing the cytofix/cytoperm on the cells while they are in >> suspension, and THEN making the cytospins? Switching to a different >> fixative? Gayle Callis had a post a while ago about using a fixative of >> 75% acetone and 25% methanol for murine CD markers. Maybe it would work >> for all your markers. I've tried modifying this fixative using propanol >> instead of ethanol for frozen sections on human tissue. It worked. >> >> When all else fails, I like to, as closely as possible, treat the >> specimen like formalin-fixed paraffin-embedded tissue. I will fix in >> formalin, dehydrate in staining dishes (like processing tissue) ...then >> bring it back to water and do antigen retrieval before staining. You >> might lose too many cells trying this on cytospins though, but might as >> well throw it out there. >> >> >> Just some thoughts... >> >> > > > > > >> Mark Adam Tarango HT(ASCP) >> >> Histology/Immunohistochemistry Supervisor >> >> Nevada Cancer Institute >> >> One Breakthrough Way >> >> Las Vegas, NV 89135 >> >> mtarango@nvcancer.org >> >> Direct Line (702) 822-5112 >> >> Fax (702) 939-7663 >> >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa >> Mazan >> Sent: Wednesday, August 08, 2007 4:16 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] immunostaining post-FACS >> >> Hi all, Wondering if anyone has done much immunostaining post FACS. WE >> have had a lot of trouble with this procedure - we collect our cells >> (from murine lung) into BSA on ice, bring them back to our lab (FACS >> is at our core facility, so it means about an hour and a half before >> the cells get to our lab). In the lab, we immediately cytospin - the >> morphology is nice on H and E - and then fix in Cytofix Cytoperm for 20 >> minutes at 4C. We either commence immunostaining immediately after, or >> leave in fridge for the next day. We do either immunofluorescence or >> immunoenzyme (usually ABC system) for identifying antigens of interest. >> We always have a negative control, but don't always have a positive >> control. We do either double staining for SPC and CC10 or single >> staining for alpha sma, vimentin, e-cad, or pancytokeratin. The >> problem that we have is that although the negative controls are very >> negative with respect to the cases, we seem to be getting a lot of >> false positives when the antibody is actually applied - for instance, >> my cells will stain 80% positive - strongly positive - using alpha sma, >> but a Western of the same cells will show no alpha-sma at all, whereas >> there is positive staining on whole lung suspensions. Any ideas? >> Advice for getting good post-FACS immunostaining? Many thanks - Melissa >> >> Melissa R. Mazan, DVM, Diplomate ACVIM >> Associate Professor and Director of Equine Sports Medicine >> Department of Clinical Sciences >> Tufts Cumming School of Veterinary Medicine >> 200 Westborough Road >> North Grafton, MA 01536 >> Tel:508-839-5395 >> Fax:508-839-7922 >> email: melissa.mazan@tufts.edu >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> "EMF " made the following annotations. >> ------------------------------------------------------------------------------ >> CONFIDENTIALITY NOTICE: This e-mail message, including any >> attachments, is for the sole use of the intended >> recipient(s) and may contain confidential, proprietary, >> and/or privileged information protected by law. If you are >> not the intended recipient, you may not use, copy, or >> distribute this e-mail message or its attachments. If you >> believe you have received this e-mail message in error, >> please contact the sender by reply e-mail and destroy all >> copies of the original message >> ============================================================================== >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > > > > > > > ------------------------------ > > Message: 14 > Date: Thu, 9 Aug 2007 09:59:47 -0500 > From: "Mike Pence" > Subject: RE: [Histonet] Slide Staining Variability > To: "Annette Hall" , > > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6AE@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > We have had some of these same problems and it is always been a nozzle > or mixer problem. Do you perform regular PM's? The reaction buffer ph > could also be off, that can cause varied staining. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette > Hall > Sent: Thursday, August 09, 2007 8:42 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide Staining Variability > > > Histonetters, > > For those of you who use automated IHC staining systems, has anyone had > issues with part of the slide staining and the rest of the slide not? We > have adopted the policy of placing the control on with the patient > tissue as a more comprehensive check of the system. However, we > occasionally find the control is acceptable but the patient fails to > even counterstain. This has been an intermittent problem on the Ventana > Benchmark for some time, but the frequency has been increasing to almost > daily. > > Any thoughts? > > Thanks, > Annette J Hall > Histo/Cyto/Micro Supervisor > United Clinical Laboratories > Dubuque, IA 52001 > > -----Original Message----- > From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] > Sent: Monday, August 06, 2007 3:35 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica autostainer w/integrated coverslipper > > Histonetters, > Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) > with the integrated coverslipper (CV5030)? Also, can you tell me if > you've experienced any trouble with it and how long you've been using > it? > > Thanks, > Sandy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE: This email may contain legally privileged information. The > information is for the use of only the intended recipient(s) even if > addressed incorrectly. If you are not the intended recipient, please > notify the sender that you have received it in error and then delete it > along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 15 > Date: Thu, 09 Aug 2007 11:08:11 -0400 > From: Pamela Marcum > Subject: [Histonet] Region II Fall Seminar for September 6, 7 and 8, > 2207 > To: histonet@lists.utsouthwestern.edu > Cc: "Gloria Limetti":; > Message-ID: <6.2.5.6.2.20070809110548.01c7c128@vet.upenn.edu> > Content-Type: text/plain; charset="iso-8859-1"; format=flowed > > > > We are moving close to the dates for the meeting > and registration is coming so please look over > the program and request your full program if you > don't have it. The following is a list of our > topics and speakers. Please let us know if you need more information. > > The meeting is at the Delaware Technical > Community College in Stanton DE. It is just off > I-95 with easy access. The Thursday is primarily > slated for management issues and topics while the > remainder of Friday and Saturday are general > topics for histology, cytology and research. If > you CEUs and can't get to Denver this is an > option for you to catch up this year. > > Region II Fall Symposium, September 6th, 7th, 8th 2007 > > Delaware Technical Community College-Stanton, DE > > Can?t get to Denver for the NSH Symposium and Convention yet still need > CEU?s? Join us for the Region II Fall Symposium in Delaware! > > Thursday Seminars*^ - will be geared toward management topics. > > 8:30 am to 11:30 am > WS #01 CPT Coding-How Do I Code This > One? Bonnie Whitaker 3 hrs > WS #02 CAP Inspection-What?s New and Required Now Charlie Dorner 3 hrs > > 1:00 pm to 4:30 pm > WS #03 Lean ? Organizing Your Life and > Lab Donna Montegue 3 hrs > > 1:00 pm to 2:30 pm > WS #04 Reducing Immunohistochemistry > Expenses Joe Myers 1.5 hrs > > 3:00 pm to 4:30 pm > WS #05 Important Consideration in Selection > of Joe > Myers 1.5 > hrs IHC > Instruments > > > Friday Seminars*^ > > 8:30 am to 12:00 pm > WS #06 Beginning IHC (new to the field or > refresher) Bonnie Whitaker 3 hrs > > 8:30 am to 10:00 am > WS #07 Delaware Coroner > Cases > Dr. Richard Callery 1.5 hrs > WS #08 Brain Diseases and Identification by > Histology Dr. Barbara Crain 1.5 hrs > WS #09 Sentinel Nodes & Breast > Biopsies Dr. Mary Iococca 1.5 hrs > > 10:30 am to 12:00 pm > WS #10 Tumor > Classification > Dr. Gary Witkin 1.0 hrs > WS #11 Deconvolution > Microscopy > Dr. Robert Akins 1.5 hrs > WS #12 Immunofluorescence for Kidney > Biopsies Dr. Robert Garola 1.5 hrs > > 1:00 pm to 4:30 pm > WS #13 Microwave > Processing Connie Wavrin 3 hrs > WS #14 Molecular > Biology > Abraham Joseph 3 hrs > > *Classes Subject to Change > ^ CEU?s Pending Approval from NSH > > > > > 1:00 pm to 2:30 pm > WS #15 Round Table- to Discuss Options in > Histology-Several Speakers will > discuss other options for careers in > histology, such as Pharmaceutical Industry, > Veterinary & Research or Industry Options for > Sales and Technical Training 1.5 hrs > WS #16 ISH, FISH, > PCR > Mitch Gore 1.5 hrs > WS #17 FNA > Cytology > Osman Ouattara 1.5 hrs > > 3:00 pm to 4:30 pm > WS #18 Bog People of Northern > Europe Sandra Olson PhD 1.5 hrs > > Saturday Seminars*^ > > 8:30 am to 12:00 pm > WS #19 IHC-Locating and Validating New Antibodies > and IHC > Reagents > Charlie Dorner 3 hrs > WS #20 > Ergonomics > Jan Minchew 3 hrs > > 8:30 am to 10:00 am > WS #21 Non-healing Lesion-Delusion or > Parasite? David Brenner 1.5 hrs > WS #22 Forensic & Medical > Entomology Carolyn & Vincent > D?Amico > 1.5 hrs > > 10:30 am to 12:00 pm > WS #23 Interesting Cases in Veterinary > Pathology Dr. Perry Habecker 1.5 hrs > WS #24 Clinical Flow > Cytometry > Brenda Rabeno 1.5 hrs > > 1:00 pm to 4:30 pm > WS #25 IHC for Animal Histology (24 > limit-wet) Zahra Naser 3 hrs > WS #26 Safety for > Histology > Sylvia Casey 3 hrs > WS #27 Grossing for > Histology Connie Wavrin 3 hrs > > 1:00 pm to 2:30 pm > WS #28 Mad Cow Disease & Other > Transmissible Dr. Michael Kanzer 1.0 hrs > Spongiform Encephalopathies > 3:00 pm to 4:30 pm > WS #29 Lab Math and > Chemistry > Donna Montegue 1.5 hrs > > 8:30 am to 4:30 pm > WS #30 QIHC > Preparation > Ethel Macrea 6 hrs > WS #31 HT > Readiness > Linda Foster-Brown 6 hrs > > > * Classes subject to change > ^ CEU?s Pending Approval from NSH > > Fees > Students & Retirees** > > ? Day Session $ > 40.00 ? Day Session $ 20.00 > Full Day Session $ > 80.00 Full Day Session $ 40.00 > > Meet and Greet- seminar attendees included, > guests $25.00 > Registration Fee $25.00 ?waived if registered by August 15th > 10% off groups of four or more from same laboratory in attendance > All workshops are subject to change > **proper student ID must be mailed with registration > ________________________________________________________________________ > > Full Program with abstracts will be available > soon. If you would like to register now please fill in the following: > > Name ______________________________________ > Address ____________________________________ > ____________________________________ > ____________________________________ > Place of employment _________________________ > Home # ________________ Work# _____________ > E-mail _____________________________________ > ________________________________________________________________________ > > Workshops planning to attend > > Thursday September 6th _________ _________ _________ > Friday September 7th _________ _________ _________ _________ > Saturday September 8th _________ _________ _________ _________ > Number planning to attend Meet and Greet ________ > Total amount enclosed ___________ > ________________________________________________________________________ > > Please send completed forms with payment to: > HSD > PO Box 5341 > Wilmington, DE 19808-0341 > > Any questions please contact: Michelle Hart at > mhart@christianacare.org > Elaine > Perkins at eperkins@christianacare.org > Kristen > Broomall at histotechkb@gmail.com > You may also contact your local histology > societies for information concerning the 2007 > Region II Fall Symposium being held at DTCC in Stanton, Delaware. > > Hotels in the area of DTCC Stanton Campus > > > Courtyard by Marriot-48 Geoffrey Dr. > 302-456-3800 > ? Block of rooms being held at > $114.00/single and $124.00/double until August 9th > Christiana Hilton-100 Continental Dr. > 302-454-1500 > ? Block of rooms being held at $129.00/single and $149.00/double until > August 6th > Days Inn-900 Churchman?s Rd. > 302-368-2400 > Country Inn and Suites-1024 Old Churchman?s Rd. > 302-266-6400 > Fairfield Inn by Marriot-65 Geoffrey Dr. > 302-292-1500 > Red Roof Inns-415 Stanton-Christiana Rd. > 302-292-2870 > Homestead Studio Suites Hotel-333 Continental Dr. > 302-283-0800 > > > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > > > > ------------------------------ > > Message: 16 > Date: Thu, 9 Aug 2007 08:09:46 -0700 > From: "Ford, Judi" > Subject: [Histonet] Masson's Troubleshooting > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good morning to all from not so sunny northern CA: > > > > I want to thank you for all the input you gave me about my problem with > the trichrome, especially to John, Gayle, Gundrun and Sarah. I > re-examined the slides and compared the control with a Gomori's One-Step > control slide, which had be stained a couple days prior. Both stains > used the same control tissue. When I first looked at the Gomori's slide > the collagen appeared to be stained well with the green. The same area > in the Masson's didn't stain at all with the aniline blue. Upon > examining both slides this morning I noticed that even though the green > stain showed there was still some red stain within it. So, we are > ordering new phosphotungstic acid and I'm cutting new slides. My > co-worker is staining Masson's this morning with some other control > slides and I'll try again with the new slides. If we're having the same > results then I'm assuming that its got to be the phosphotungstic acid > and we'll try again when the new stuff arrives. > > > > Anyway, I really appreciate all of your suggestion and input. This board > has always been a valuable resource for me. > > > > Cheers! > > Judi Ford > > Palo Alto, CA > > > > ------------------------------ > > Message: 17 > Date: Thu, 9 Aug 2007 10:22:58 -0500 > From: "Johnson, Teri" > Subject: [Histonet] Job Posting - Research Histology, Kansas City, MO > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > The Stowers Institute for Medical Research has an opening for a > Histology Specialist II to provide high quality research histology > services. > > Responsibilities include sample preparation including sample > receipt, fixation, processing, embedding, microtomy and staining; > operation and maintenance of histology prep and ancillary equipment; > training researchers in the use of common equipment and preparation > techniques; and development of protocols for specialized research > histology-related projects. > > In addition to the ability to effectively function in a > team-oriented environment, the successful candidate must have fine-motor > skills to manipulate tiny tissue samples and to demonstrate histology > techniques; excellent communication skills to interact with the research > scientists; a familiarity with basic biology, cell biology or genetics; > and a record of excellent general laboratory skills. > > Minimum requirements include an Associates Degree in Biology or a > related field and two years experience in basic research using histology > techniques. Preference will be given to individuals with a Bachelors > Degree in a related field, and or additional experience in basic > research histology. > > For benefits information and to apply: > http://www.stowers-institute.org/ScientistsSought/ScientistsSought.asp#r > esume > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > > > ------------------------------ > > Message: 18 > Date: Thu, 9 Aug 2007 08:58:31 -0700 > From: "Human Resources" > Subject: [Histonet] histology contractor needed > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=WINDOWS-1252 > > Temporary Histology Contractor needed ? 4-6 months > > > > We are a well-known biotechnology company located in Silicon Valley, CA. We > are looking for an experienced histotechnician to help out in our histology > lab in a dynamic research setting. The successful candidate should have > experience with basic histology procedures such as tissue trimming, > processing, paraffin embedding, cutting, and staining with H&E, as well as > experience with common special stains, and a facility with cryosectioning. > Qualified candidates must have a minimum of 3-5 years relevant experience. > Certification in HT and in phlebotomy would be a plus. > > > > To apply, please send resume to > *histocontract@gmail.com* > *,* along with desired hourly rate of pay. > > > ------------------------------ > > Message: 19 > Date: Thu, 9 Aug 2007 11:08:21 -0500 > From: "Renko, Heather D." > Subject: [Histonet] NSH > To: histonet@lists.utsouthwestern.edu > Message-ID: > <40026EDDE64CDA47AB382C52619ACD3C0777558A@pmc-rfd-mx01.intranet.osfnet.org> > > Content-Type: text/plain; charset=iso-8859-1 > > I received my packet in June and registered. Check your membership expiration possibly? > > Heather Renko, Histology Coordinator > OSF Saint Anthony Medical Center > 5666 East State Street > Rockford, Illinois 61108 > 815-395-5410 > Heather.D.Renko@osfhealthcare.org > > > ============================================================================== > The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. > ============================================================================== > > > ------------------------------ > > Message: 20 > Date: Thu, 9 Aug 2007 12:23:24 -0400 > From: "Wilson, Carol" > Subject: [Histonet] Blocking arrangements in research for different > species > To: > Message-ID: > <9D443EB9D0270143B5AAF190CB1A58A305064EB2@dogwood.ricerca.com> > Content-Type: text/plain; charset="us-ascii" > > I was wondering if anyone out there might be willing to share their > blocking arrangement for different species in research with me via email > or fax. Or a specific resource that I could find online, book, etc.. > I've googled without much success so far. I need somewhere to start and > any and all help would be appreciated. > > Thanks, > > Carol > > > > Carol Wilson > > Team Leader - Histology > > Ricerca Biosciences, LLC > > 7528 Auburn Rd. > > Concord, OH 44077 > > 440-357-3930 > > > > > > ------------------------------ > > Message: 21 > Date: Thu, 9 Aug 2007 11:51:02 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Slide Staining Variability > To: "Mike Pence" , "Annette Hall" > , > Message-ID: <003d01c7daa5$7a4f8800$d49eae18@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > we have had the same problem on all three machines. To date, it has been: > technician error, failure to remove a yellow locking ring, vortex mixer, > slide warmer not hot enough, slide warmer too hot, dispenser not working, > Venus is not aligned with Mars. Most of the time we have had to get a > service rep out to repair something or other. One machine has had the > carousel replaced twice. Need I say more? > Oh yeah, I know you're here, go call my employer like you did last time. > Oh, I should say "former" employer. > Once again, the views of this editorial are my own and I have not been > coerced, drugged, or beaten with a feather. > > JTT > ----- Original Message ----- > From: "Mike Pence" > To: "Annette Hall" ; > > Sent: Thursday, August 09, 2007 9:59 AM > Subject: RE: [Histonet] Slide Staining Variability > > > We have had some of these same problems and it is always been a nozzle > or mixer problem. Do you perform regular PM's? The reaction buffer ph > could also be off, that can cause varied staining. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette > Hall > Sent: Thursday, August 09, 2007 8:42 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide Staining Variability > > > Histonetters, > > For those of you who use automated IHC staining systems, has anyone had > issues with part of the slide staining and the rest of the slide not? We > have adopted the policy of placing the control on with the patient > tissue as a more comprehensive check of the system. However, we > occasionally find the control is acceptable but the patient fails to > even counterstain. This has been an intermittent problem on the Ventana > Benchmark for some time, but the frequency has been increasing to almost > daily. > > Any thoughts? > > Thanks, > Annette J Hall > Histo/Cyto/Micro Supervisor > United Clinical Laboratories > Dubuque, IA 52001 > > -----Original Message----- > From: Harrison, Sandra C. [mailto:Sandra.Harrison3@va.gov] > Sent: Monday, August 06, 2007 3:35 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica autostainer w/integrated coverslipper > > Histonetters, > Could anyone tell me if they're using the Leica AutoStainer XL (ST5010) > with the integrated coverslipper (CV5030)? Also, can you tell me if > you've experienced any trouble with it and how long you've been using > it? > > Thanks, > Sandy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE: This email may contain legally privileged information. The > information is for the use of only the intended recipient(s) even if > addressed incorrectly. If you are not the intended recipient, please > notify the sender that you have received it in error and then delete it > along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 45, Issue 13 > **************************************** > From christiegowan <@t> msn.com Fri Aug 10 08:51:47 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Aug 10 08:52:07 2007 Subject: [Histonet] Slide Staining Variability In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C012B6054@mercury.pa-ucl.com> Message-ID: Hi Annette, I have heard of this problem and all the answers to date could be your problem. I would like to add one more scenario. If one is using the slides with the red paint box, we have heard that during the summer months the heat can affect this paint border and create a wall that will not allow the mixer to push the solutions down past the control. We use super frost plus slides and whenever we have the problem you are describing it is usually the vortex mixer. Hope this helps. Happy Friday All! From Charlene.Henry <@t> STJUDE.ORG Fri Aug 10 09:22:23 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Aug 10 09:22:41 2007 Subject: [Histonet] Dehydrating beads. . In-Reply-To: <001201c7f354$4f026d30$6501a8c0@CHERYLSLAPTOP> Message-ID: <5CB39BCA5724F349BCB748675C6CA1A21454354D@SJMEMXMB02.stjude.sjcrh.local> Where do you buy these dehydrating beads? Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Sunday, September 09, 2007 9:43 PM To: 'Webb, Dorothy L'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dehydrating beads. . Hey-- I used these in a lab where I temped a few weeks ago and I LOVE THEM!! I've been in a lot of labs and used a lot of different stainers and this was a big X-Y Leica with the upright slide racks. When staining the xylene is stirred up with the movement of the slides and the beads enabled us to handle less xylene (exposure from rotating) and the slides when slipped appeared to clear under the coverslips both faster and more thoroughly. To dry them you lay them near your air cleaner as you leave for the day so you don't breathe it, and you just need enough to rotate them through each day. My two cents! Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.883.7704 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, August 08, 2007 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dehydrating beads I know this was discussed in the recent past, but, what are the pros and cons of using these and how much do they save on xylene usage? Also, how does one get into the archives of Histonet to view past responses if those of you who already answered do not want to post an answer agin (and I do understand not rehashing old subjects!!) I cannot say enough how VERY much I appreciate the use of this resource for problem solving in the histology world!! The networking is invaluable and I want to thank everyone out there for the quick responses and helpful information on my last post concerning automated microtomes!!!!!! Histotechs are a truly a CUT above the rest!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Fri Aug 10 09:22:51 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Aug 10 09:23:10 2007 Subject: [Histonet] mouse skin processing for paraffin embedding Message-ID: Dear All, I have problems sectioning mouse skin and tail. My protocol is : 4% PFA 16h at 4 C wash in PBS 70% Ethanol 30 min RT 85% Ethanol 30 min RT 95% Ethanol 30 min RT 100% Ethanol 2X 30 min each RT histoclear 3X 30 min each RT 1:1 paraffin-histoclear 45 min at 60C oven paraffin 3X 30 min each at 60 C oven w/ vacuum. embedding I would like to know if skin in particular requires something different in terms of processing or even sectioning. Thank you for your valuable opinions. Cheers, Mesruh Turkekul Memorial Sloan-Kettering Cancer Center New YORK 646 888 2209 On 7/22/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Problems with the Histonet? (Dolores Townsend) > 2. MWO validation. (Rene J Buesa) > 3. Shandon Sequenza(r) Immunostaining Center (Igor Deyneko) > 4. Placenta returns (Bryan Llewellyn) > 5. Question? (Maribel Santiago) > 6. paraffin heater (Steven Coakley) > 7. Looking for Angie Bailey (Victoria Baker) > 8. No posts (machocraig) > 9. Listeria By Immunohistochemistry (Marilyn Johnson) > 10. Re: Placenta returns (Joe Nocito) > 11. Re: Question? (Joe Nocito) > 12. RE: Unsuccessful Muscle Lipid Staining (Liz Chlipala) > 13. RE: paraffin heater (Weems, Joyce) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 20 Jul 2007 11:31:03 -0400 > From: "Dolores Townsend" > Subject: [Histonet] Problems with the Histonet? > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I have not received any message from the Histonet since Wednesday > morning. Anyone else having this problem? > Dolores > > > ------------------------------ > > Message: 2 > Date: Fri, 20 Jul 2007 08:45:46 -0700 (PDT) > From: Rene J Buesa > Subject: [Histonet] MWO validation. > To: Patsy Ruegg , > histonet@lists.utsouthwestern.edu > Message-ID: <484779.21679.qm@web61221.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Patsy: > I completely agree with you. A few days ago I advised an MD do to hold > using MWO for breast that was going to be used with IHC tests. > My argument was as follows: > 1- FDA has approved Her2Neu for FFPE tissues, and they say NOTHING about > MWO although it could be assumed that it was using conventional processig > 2-DAKO developed their protocol (later approved by FDA) using conventional > tissue processors > 3- IF something happens and a lawsuit is brought, any savy lawyer (and > they are savy enough) could "dig-out" the type of processing used and IF it > was MWO it is likely that their argument could be accepted by a jury. > I don't think that a lab individual validaiton could hold in court > (against an FDA approved procedure). NEW guidelines by the FDA would be > needed (as you point out). > MWO users beware! > Ren? J. > > Patsy Ruegg wrote: > Rene, > Has mw processing been validated for IHC (especially for her2neu) by > running > 25-100 cases side by side (one piece conventionally processed and the > other > mw processed from each of the 25-100 cases) then running the IHC? Until > this is done and reported the new guidelines for her2 testing will require > that any deviation from conventional processing and fixation for 6-48hr > using the FDA approved her2neu kits, labs using mw processing will not be > in > compliance. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Tuesday, July 17, 2007 10:17 AM > To: Weaver, Colin; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microwave v conventional processing > > Colin: > Using the adequate protocols MW processing renders equivalent results to > "conventional" tissue processing, that is the general concensus. > The thing is that unless you use an automated MW tissue processor, a > histotech will have to attend to the process and change reagents manually. > This can lead to 2 problems: higher exposure of the HT to (usually hot) > chemicals and some degree of inconsistency in the protocol because the > time > in each reagent could vary slightly different between runs. > Consider that a few minutes in a conventional protocol is a much lower > percentage of the time in the reagent, than the same amount of time in a > much faster protocol completed with a MW tissue processor. > MW processing should be an option when TAT is an issue and even then there > are numerous manual steps independent of the time the tissue is involved > in > the processing step; they are independent of the processing technology and > usually count for the greater part of the total TAT. > Under separate cover I am sending you an article of mine wher I analyze > this issue. > Ren? J. > > "Weaver, Colin" wrote: > Hi - we are trying to go down the microwave route in processing but > inevitably some of our veterinary pathologists are questioning whether > microwave sections are as "good" as conventional processing. Can anyone > point me in the right direction to find any comparison done between > microwave processing and conventional overnight processing with regard > to section and staining quality. > > > Colin Weaver > Veterinary Laboratories Agency (VLA) > > This email and any attachments is intended for the named recipient > only. > If you have received it in error you have no authority to use, disclose, > store or copy any of its contents and you should destroy it and inform > the sender. > Whilst this email and associated attachments will have been checked > for known viruses whilst within VLA systems we can accept no > responsibility once it has left our systems. > Communications on VLA's computer systems may be monitored and/or > recorded to secure the effective operation of the system and for other > lawful purposes. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user > panel > and lay it on us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------- > Need a vacation? Get great deals to amazing places on Yahoo! Travel. > > ------------------------------ > > Message: 3 > Date: Fri, 20 Jul 2007 11:46:06 -0400 > From: "Igor Deyneko" > Subject: [Histonet] Shandon Sequenza(r) Immunostaining Center > To: histonet@lists.utsouthwestern.edu > Message-ID: > <35e16a770707200846v4c873163v6cbda858caa93950@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hello. > I was just wondering whether or not someone uses Shandon Sequenza(r) > Immunostaining Center for IHC? The reason i am asking is that do you use > it > for all your IHC or in any specific cases. In my experience, I found that > 2 > slices of tissue on the same slide get different amount of reagents, so > when > viewed under the microscope, they light up differently, showing different > levels of saturation with a chromogen. > Thank you. > Igor Deyneko. > Infinity Pharmaceutical > Cambridge,MA. > > > ------------------------------ > > Message: 4 > Date: Fri, 20 Jul 2007 09:42:50 -0700 > From: Bryan Llewellyn > Subject: [Histonet] Placenta returns > To: Histonet > Message-ID: <000501c7caed$02beb120$ff144246@yourlk4rlmsu> > Content-Type: text/plain; format=flowed; charset=iso-8859-1; > reply-type=original > > I thought this might be of interest. We had a discussion on this issue > several months ago, I believe. > > > http://www.ctv.ca/servlet/ArticleNews/story/CTVNews/20070720/placenta_hospital_070720/20070720?hub=Health > > Bryan Llewellyn > > > > > ------------------------------ > > Message: 5 > Date: Fri, 20 Jul 2007 18:45:26 +0000 > From: Maribel Santiago > Subject: [Histonet] Question? > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > What is going on with histonet? I'm not receiving emails since wednesday > afternoon! Minnie > _________________________________________________________________ > Express yourself instantly with MSN Messenger! Download today it's FREE! > http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ > > ------------------------------ > > Message: 6 > Date: Fri, 20 Jul 2007 16:18:10 -0700 (PDT) > From: Steven Coakley > Subject: [Histonet] paraffin heater > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20070720231810.88168.qmail@web38203.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I'm looking for the maker of a heater used to remove paraffin from the > sides of paraffin tissue cassettes. I used one a a facility I temped at in > Milwaukee but didn't get the manufacturer. > > Thanks, > > Steve > > > --------------------------------- > Be a better Globetrotter. Get better travel answers from someone who > knows. > Yahoo! Answers - Check it out. > > ------------------------------ > > Message: 7 > Date: Sat, 21 Jul 2007 10:29:40 -0400 > From: "Victoria Baker" > Subject: [Histonet] Looking for Angie Bailey > To: "Histo Net list server" > Message-ID: > <4f016b690707210729v4e91e191qab17916456c2a662@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi > > I am posting this for a friend if anyone know how I can locate Angie > Bailey, please let me know! > > Thanks in advance. > > Vikki Baker > > > > ------------------------------ > > Message: 8 > Date: Sat, 21 Jul 2007 14:41:39 -0600 > From: "machocraig" > Subject: [Histonet] No posts > To: > Message-ID: > Content-Type: text/plain; format=flowed; charset="Windows-1252"; > reply-type=original > > Hi, > I am not getting any posts. > Is histonet out of commission? > > Craig > > > > ------------------------------ > > Message: 9 > Date: Sun, 22 Jul 2007 07:26:03 -0600 > From: "Marilyn Johnson" > Subject: [Histonet] Listeria By Immunohistochemistry > To: > Message-ID: <000801c7cc63$da331d40$6401a8c0@VALUED20606295> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > Thanks to all of you who replied to my request. > Greatly appreciated. > > Marilyn Johnson > Food Safety Division > Alberta Agriculture > Edmonton, AB. Canada > > ------------------------------ > > Message: 10 > Date: Sun, 22 Jul 2007 09:10:42 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Placenta returns > To: "Bryan Llewellyn" , "Histonet" > > Message-ID: <004401c7cc6a$176b0dc0$d49eae18@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=response > > Bryan, > two things. First, the placenta was frozen and not placed in formalin. > Every > hospital I have worked in always placed the placenta in formalin (ok, some > might have placed 15 mls in the container, maybe 20). The second thing I > noticed was that Nevada has no law about returning specimens to the owner. > I've worked at placed were we were being sued by a guy because he > wanted > his finger back, 18 months ago. Even though we sent him copies of he > Texas > law and MSDS on 10% NBF, he still found a sleazy lawyer to file suit. > Also, I've had experiences where the patient has requested their > specimen back because of religious reasons. So here's my point. People who > want their specimens back because of religious reasons do so up front. > People who want to place their specimen on the coffee table for a > conversational piece will request it back as an after thought. Like Billy > Bob who wanted his finger back. "Hey Bubba, look what I took home from the > hospital". "No you idiot, it's not chicken liver, it's my hemorrhoids". > That's my story and I'm sticking to it. > > JTT > ----- Original Message ----- > From: "Bryan Llewellyn" > To: "Histonet" > Sent: Friday, July 20, 2007 11:42 AM > Subject: [Histonet] Placenta returns > > > >I thought this might be of interest. We had a discussion on this issue > >several months ago, I believe. > > > > > http://www.ctv.ca/servlet/ArticleNews/story/CTVNews/20070720/placenta_hospital_070720/20070720?hub=Health > > > > Bryan Llewellyn > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 11 > Date: Sun, 22 Jul 2007 09:13:00 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Question? > To: "Maribel Santiago" , > > Message-ID: <005701c7cc6a$69ae9fc0$d49eae18@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > I think the Histonet went on vacation. Hey, it is summer you know. Except > in > Texas, it's monsoon season. I picked a real winner of a summer to take > off. > Okay, if you must know, I AM walking around with the a big L on my > forehead. > > JTT > ----- Original Message ----- > From: "Maribel Santiago" > To: > Sent: Friday, July 20, 2007 1:45 PM > Subject: [Histonet] Question? > > > What is going on with histonet? I'm not receiving emails since wednesday > afternoon! Minnie > _________________________________________________________________ > Express yourself instantly with MSN Messenger! Download today it's FREE! > > http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 12 > Date: Sun, 22 Jul 2007 10:13:27 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] Unsuccessful Muscle Lipid Staining > To: "Son Quang Duong" , > > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > Son > > We did this in the past, we post fixed in osmium, it turned out quite > nice. I'll send images in a different e-mail. We fixed in formalin and > then post fixed the muscle in osmium overnight, we were working on mouse > tibialis anterior so we kept the entire muscle intact and processed the > whole muscle and then cut the muscle in half prior to embedding. We found > that the unstained sections turned out the best for demonstration of the > fat. I might have a material and methods some where since I think they > published on it. I'll send that to you with the images. > > Liz > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sonny Duong > Sent: Sunday, July 22, 2007 3:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Unsuccessful Muscle Lipid Staining > > Hi all, > > I have been trying to stain mouse muscle tissue for intramuscular > triglyceride droplets with Oil Red O (dissolved in triethyl phosphate) for > some time now, and my results have been disastrous. I've tried it on both > fresh frozen and formaldehyde fixed tissues (and cryoprotected) > cryosections, but almost always it seems that the lipid droplets within the > fibers (maybe?) leak out of the cells and conglomerate on the edges of the > tissue and within gaps between the cells. Very few of the fibers, if any, > display any lipid droplet staining and it is always very weak. The lipid > droplets all conglomerate in roughly the same positions between serial > sections as well, which leads me to believe something is happening during > the freezing/fixation process, or the cutting in the cryosection. Does > anyone have any suggestion as to what I could be doing wrong here (i.ecutting technique, temperature, fixatives)? Also, does anyone have any > experience with using ORO in triethyl phosphate? > > Thank you all for your time, > Sonny Duong > University of Virginia > Green Lab > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.476 / Virus Database: 269.10.12/910 - Release Date: 7/21/2007 > 3:52 PM > > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.476 / Virus Database: 269.10.12/910 - Release Date: 7/21/2007 > 3:52 PM > > > > > ------------------------------ > > Message: 13 > Date: Sun, 22 Jul 2007 12:43:11 -0400 > From: "Weems, Joyce" > Subject: RE: [Histonet] paraffin heater > To: "Steven Coakley" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9EE6@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="us-ascii" > > ThermoFisher Scientific - formerly > ThermoElectronFisherScientificThermoShandonShandonLipshawLabVision... > Etc! > I would look on the web site. I'm not sure if the product number changed > after all the merges... > > Good luck! j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven > Coakley > Sent: Friday, July 20, 2007 7:18 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] paraffin heater > > I'm looking for the maker of a heater used to remove paraffin from the > sides of paraffin tissue cassettes. I used one a a facility I temped at > in Milwaukee but didn't get the manufacturer. > > Thanks, > > Steve > > > --------------------------------- > Be a better Globetrotter. Get better travel answers from someone who > knows. > Yahoo! Answers - Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of this > information is strictly prohibited. If you have received this communication > in error, please notify us immediately by replying to the message and > deleting it from your computer. Thank you. Saint Joseph's Health System, > Inc. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 44, Issue 22 > **************************************** > From christiegowan <@t> msn.com Fri Aug 10 10:25:13 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Aug 10 10:25:29 2007 Subject: [Histonet] Slide Staining Variability Message-ID: I would like to make a clarification. The slides with the red boxes are placed front to back in the slide boxes. There is speculation that heat may cause some kind of transfer to the front of the slide that creates a barrier. If you could stop the process right after hematoxylin you would see all the dye pooled at the top right over the control tissue. I have seen pictures of this actually happening. I would also like to mention that I used those slides for years at another facility on the Nexes with no problems. Thanks Clarissa for your private correction. Someone needs to keep an eye on me! >From: "CHRISTIE GOWAN" >To: Annette_hall@pa-ucl.com, Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Slide Staining Variability >Date: Fri, 10 Aug 2007 13:51:47 +0000 > > > >Hi Annette, >I have heard of this problem and all the answers to date could be your >problem. I would like to add one more scenario. If one is using the slides >with the red paint box, we have heard that during the summer months the >heat can affect this paint border and create a wall that will not allow the >mixer to push the solutions down past the control. We use super frost plus >slides and whenever we have the problem you are describing it is usually >the vortex mixer. Hope this helps. Happy Friday All! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Aug 10 11:34:27 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Aug 10 11:34:32 2007 Subject: [Histonet] mouse skin processing for paraffin embedding In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CC7@LSRIEXCH1.lsmaster.lifespan.org> I believe the times you listed are too short for processing skin adequately. I suspect your sections are separating on the water bath? This is because the fat has not been completely removed, and the paraffin has not adequately infiltrated. When I process mouse skin, I use 3 hours per station - 70% ethanol x1; 95% ethanol x1; 100% ethanol x4; xylene x3; paraffin x4. I use a VIP processor, with vacuum on all stations. I have no problem sectioning skin processed this way. From immrstambo <@t> hotmail.com Fri Aug 10 12:14:36 2007 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Fri Aug 10 12:15:44 2007 Subject: [Histonet] Automated microtome In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270563507E@hpes1.HealthPartners.int> Message-ID: I use the Leica 2255 and we also have a tech that uses the 2155. Most all tissues cut excellently. Bone and some other hard tissues have a tendency to chatter as with any microtome.Is everything tight and does she soak her tissues long enough? Sometimes tissues that dont sit on icewater for 10-20 minutes chatter because the tissue is dry. That's my input. Sincerely and Good Luck, Christine Tambasco, HT (ASCP) St. Marys Hospital at Amsterdam, New York ______________________________________________________________ From: "Webb, Dorothy L" To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated microtome Date: Wed, 08 Aug 2007 12:43:41 -0500 >I am working as a supervisor at a facility that had a Leica 2155 >automated microtome. Only having had experience with manual microtomes, >I need to ask a question concerning the reliability that others find >with these microtomes. Does everyone else who uses this particular one >find it to cut all types of tissue with consistency? I only have one >tech who uses this piece of equipment due to ergonomic issues and I >cannot seem to ascertain if the problems that are encountered are tech >related or the microtome itself. I would appreciate any feedback you >could give me on your experiences with the Leica 2155, other automated >microtomes on the market in comparison, etc. As always, thank you for >your help!!! > >Dorothy Webb, HT (ASCP) >Histology Technical Supervisor >Regions Hospital, Pathology Department >640 Jackson Street, Saint Paul, MN 55101-2595 >Phone: 651-254-2962 >Fax: 651-254-2741 >Regions Hospital is part of the HealthPartners family of care >________________________________________ >This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > >If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Messenger Café open for fun 24/7. Hot games, cool activities served daily. Visit now. References 1. http://g.msn.com/8HMBENUS/2734??PS=47575 From gcallis <@t> montana.edu Fri Aug 10 12:20:43 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Aug 10 12:20:48 2007 Subject: Ethanol versus methanol for CD marker fixation RE: [Histonet] immunostaining post-FACS In-Reply-To: <000f01c7db08$b6316420$4d00a8c0@patologija.mf> References: <20070808191629.vfq6wu4h6o0kccko@webmail.tufts.edu> <5AEC610C1CE02945BD63A395BA763EDE011B6DB8@NVCIEXCH02.NVCI.org> <6.0.0.22.1.20070809082551.01b37dd8@gemini.msu.montana.edu> <000f01c7db08$b6316420$4d00a8c0@patologija.mf> Message-ID: <6.0.0.22.1.20070810104502.01b845e0@gemini.msu.montana.edu> The reason I answered Mark Tarango's email in the first place was to correct which alcohol I do use with acetone (100% ethanol). It is known that methanol is not optimal for all antigens, and we avoid it with our murine CD markers. It has been noted in the literature that CD marker staining may be compromised by fixation with methanol and certainly true of our murine CD markers (Elias book, the book is at home). This is probably due to the hydrolysis of the protein antigen by methanol, and probably happens with ethanol too. There was a Histonet discussion some years back (in Histonet archives) on the use of methanol for CD marker fixation, and it was noted by some that loss of staining after methanol did occur for some CD markers they were working with. Human CD4 and CD8 do not work after ethanol fixation, and probably will be compromised badly by the acetone/ethanol mixture. I believe Dr. Chris van der Loos in The Netherlands tried this. He uses acetone for those markers We recently did a little inhouse study to compare the effects of methanol, acetone, acetone/absolute ethanol mixture for immunofluorescent staining of cell cultures infected with a bacteria. In this case, we had less fluorescence with methanol than with acetone, and had even better staining acetone/absolute ethanol compared to acetone. The point is that doing a fixation panel is always wise, particularly when a new protocol is being set up - different fixatives and times with those fixatives). Human CD4 and CD8 do not stain after ethanol fixation ( a fact Dr. Chris van der Loos brings up frequently, and consequently these human markers will be compromised by the acetone/ethanol mixture. I believe Dr. Chris van der Loos in The Netherlands tried this and continues to use acetone for those markers. As long as the fixation method works for you, then you may not want to change or try anything different. Question: have you ever tried another fixative as a comparison? If you do, let us know the results. At 10:40 PM 8/9/2007, you wrote: >Hi Gayle, >It's quite interesting that on the contrary we have a very good experience >with methanol as a fixative for variety of antigens (immuno on >cytospins)including CD's and nuclear antigens. Fixation in methanol >preserves good cell morphology and yield a reliable (repeatable) >immunoreactions confirmed by flow cytometry. >I'm just trying to figure out where is the reason for such a difference? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From SDrew <@t> uwhealth.org Fri Aug 10 13:27:28 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Fri Aug 10 13:27:41 2007 Subject: [Histonet] D-PAS Message-ID: When performing a D-PAS stain, do you run a PAS control also? With or without patient tissue? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From mtarango <@t> nvcancer.org Fri Aug 10 13:29:46 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Aug 10 13:30:04 2007 Subject: Ethanol versus methanol for CD marker fixation RE: [Histonet] immunostaining post-FACS In-Reply-To: <6.0.0.22.1.20070810104502.01b845e0@gemini.msu.montana.edu> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6DD1@NVCIEXCH02.NVCI.org> Sorry about Gayle, I meant ethanol but somehow typed methanol in that other post. I've tried modifying Gayle Callis' 75% acetone and 25% ethanol fixative for murine CD markers, by replacing the ethanol with propanol for staining human tissue. CD3 stains very nicely. I have a vague memory of staining CD4 after using this fixative for frozen sections, but I can't remember for sure. I'm also unsure if this would work for other CD markers, but it worked the best of all the fixative formations I tried for CD3. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, August 10, 2007 10:21 AM To: Irena KIRBIS; Histonet@lists.utsouthwestern.edu Subject: Ethanol versus methanol for CD marker fixation RE: [Histonet] immunostaining post-FACS The reason I answered Mark Tarango's email in the first place was to correct which alcohol I do use with acetone (100% ethanol). It is known that methanol is not optimal for all antigens, and we avoid it with our murine CD markers. It has been noted in the literature that CD marker staining may be compromised by fixation with methanol and certainly true of our murine CD markers (Elias book, the book is at home). This is probably due to the hydrolysis of the protein antigen by methanol, and probably happens with ethanol too. There was a Histonet discussion some years back (in Histonet archives) on the use of methanol for CD marker fixation, and it was noted by some that loss of staining after methanol did occur for some CD markers they were working with. Human CD4 and CD8 do not work after ethanol fixation, and probably will be compromised badly by the acetone/ethanol mixture. I believe Dr. Chris van der Loos in The Netherlands tried this. He uses acetone for those markers We recently did a little inhouse study to compare the effects of methanol, acetone, acetone/absolute ethanol mixture for immunofluorescent staining of cell cultures infected with a bacteria. In this case, we had less fluorescence with methanol than with acetone, and had even better staining acetone/absolute ethanol compared to acetone. The point is that doing a fixation panel is always wise, particularly when a new protocol is being set up - different fixatives and times with those fixatives). Human CD4 and CD8 do not stain after ethanol fixation ( a fact Dr. Chris van der Loos brings up frequently, and consequently these human markers will be compromised by the acetone/ethanol mixture. I believe Dr. Chris van der Loos in The Netherlands tried this and continues to use acetone for those markers. As long as the fixation method works for you, then you may not want to change or try anything different. Question: have you ever tried another fixative as a comparison? If you do, let us know the results. At 10:40 PM 8/9/2007, you wrote: >Hi Gayle, >It's quite interesting that on the contrary we have a very good experience >with methanol as a fixative for variety of antigens (immuno on >cytospins)including CD's and nuclear antigens. Fixation in methanol >preserves good cell morphology and yield a reliable (repeatable) >immunoreactions confirmed by flow cytometry. >I'm just trying to figure out where is the reason for such a difference? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Jason.Wiese <@t> va.gov Fri Aug 10 13:38:25 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri Aug 10 13:41:30 2007 Subject: [Histonet] D-PAS In-Reply-To: References: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12BC3@VHAV20MSGA3.v20.med.va.gov> If you are running something like a liver panel, the PAS or PAS-D has an internal control. When running a PAS-D, you should run a PAS for comparison on the same tissue. If you are running... lets say skin for fungus or something, then yes... always a control. At least that is my procedure... :) Jason E. Wiese HT/PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Sally A. Sent: Friday, August 10, 2007 11:27 AM To: Histonet Subject: [Histonet] D-PAS When performing a D-PAS stain, do you run a PAS control also? With or without patient tissue? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karling <@t> usc.edu Fri Aug 10 15:01:30 2007 From: karling <@t> usc.edu (Kar Ling) Date: Fri Aug 10 15:01:41 2007 Subject: [Histonet] mouse spinal cord atlas Message-ID: Hi, I have problems in defining ventral roots of lumbar spinal segments in mice. I try to look up some mouse anatomy book, but it only has bunch of spinal cord cross section. For gross anatomy of the spinal cord, I can only find rat ones. I wonder if there's such book with whole spinal cord illustration. My first questions is: Do mice has L1-L6 and S1-S4 as in rat? My way to dissect spinal cord is cutting the dorsal bones piece by pieces, and then carefully remove the ventral bones starting at the sides so that I can preserve DRG attached to both ventral and dorsal roots. (that took me an hour to expose sacral and lumber segments). If I count from conus medullaris, how can I be sure my counting is correct? should I count main nerves or emerging branches? Is there any hallmarks that can differentiate L6 from S1? According to what I see in mouse spinal cord, the last thick ventral spinal nerve coming out from the spinal cord appears to be several branches emerging from the cord then merging into one, but then split into 2 again. One of those nerves attach another dorsal root and DRG, yet I fail to preserve the other DRG if any. As for the 2 ventral nerves below this one are much thinner and also has few emerging branches from the cord. So, I am not sure about what I see is L6 or it's a merge of L6 & S1?? If I count the afforementioned nerve as L6, I can locate 3 pairs of long dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above. Yet, L1 roots are notably longer than Thoracic root along rib cage level. So, Is L1 always longer than T12?? I read some histonet achieve suggesting saline injection to push the spinal cord out, can the DRG be preserved? Thank you. Karen Ling University of Southern California Program in Neuroscience 3641 Watt Way, HNB 209 Los Angeles, CA 90089-2520 From Gina_Hendron <@t> ssmhc.com Fri Aug 10 16:13:46 2007 From: Gina_Hendron <@t> ssmhc.com (Gina_Hendron@ssmhc.com) Date: Fri Aug 10 16:14:29 2007 Subject: [Histonet] Job Opportunity Message-ID: Our multi-specialty laboratory is seeking a dynamic individual to join our progressive management team. Accountabilities include: supervising a staff of 24 who provide support services in our surgical pathology section; communicate and organize work flow, coach, motivate and positively influence staff to affect changes. Qualified candidate should have a B. S. Degree in one of the biological sciences inclusive of courses in anatomy and physiology. A minimum of four years of previous clinical laboratory experience is required. Graduate of an accredited Pathology Assistant program and previous Supervisory experience preferred. Apply on-line at www.stmarysmadison.com St. Mary's Hospital Human Resources Department 707 S. Mills St. Madison, Wisconsin 53715 ----------------------------------------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From jkiernan <@t> uwo.ca Fri Aug 10 23:36:13 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Aug 10 23:36:39 2007 Subject: [Histonet] mouse spinal cord atlas Message-ID: Dear Kar Ling, I entered ANATOMY MOUSE BOOK in Google, and the top item (of many) was http://www.informatics.jax.org/cookbook/chapters/contents2.shtml This is an atlas of drawings, covering the skeleton, vascular system and internal organs, by Margaret Cook, published originally in 1965. It does not include the nervous system. The sacrum is clearly shown to consist of 4 fused vertebrae. That answers one of your questions. The forward to Cook's book (now free, even though it was published by Academic Press) is by one W.Lane-Petter, who was already the editor of a big book on the biology of animals used in research. I recall his visit to the Anatomy Department at Birmingham University (UK) when I was a student there in 1962. I had consulted his revered book for some information about hedgehogs, the species in which I was investigating possible cholinergic mechanisms in hypothalamic neurosecretion, under the supervision of the late Dr R.L.Holmes. All this has nothing to do with dorsal roots in mice, but it's an opportunity to acknowledge Bob Holmes for showing me that histochemistry was a worthwhile pastime. (Enzyme activities were the cutting edge in those days.) Thanks pm, Bob H! The Google search for ANATOMY MOUSE BOOK brought up more than a million hits. Of those in the first fifty, many obviously were other references to Cook (1965). Your email (quoted below) indicates that you can do the difficult dissection of spinal nerves quite quickly. I know an experienced anatomist who takes a week to dissect out a fixed mouse spinal cord. The "biochemists' method" of ejecting the rat's spinal cord - removal of the head followed by injection of saline into the sacral hiatus - preserves some of the cauda equina, but it does not allow accurate identification of segmental levels. See Meikle & Martin 1981: Stain Technology 5(4):235-237 (1981) also Acta Morphologica Neerlando-Scandinavica 23: 357?368 Lumbar, sacral and caudal dorsal root ganglia are probably not all ejeccted in the biochemists' method for collecting rodents' spinal cords. Check the refs above. You are in a position to provide and publish a new and detailed description of lubosacral neves in the mouse. Do it! Submit a paper to the Journal of Aatomy. John Kiernan Anatomy, UWO Lonon, Canada --- ----- Original Message ----- From: Kar Ling Date: Friday, August 10, 2007 16:03 Subject: [Histonet] mouse spinal cord atlas To: histonet@lists.utsouthwestern.edu > Hi, > > I have problems in defining ventral roots of lumbar spinal > segments in mice. I try to look up some mouse anatomy > book, but it only has bunch of spinal cord cross section. > For gross anatomy of the spinal cord, I can only find rat > ones. I wonder if there's such book with whole spinal cord > illustration. > My first questions is: Do mice has L1-L6 and S1-S4 as in rat? > > My way to dissect spinal cord is cutting the dorsal bones piece > by pieces, and then carefully remove the ventral bones starting > at the sides so that I can preserve DRG attached to both ventral > and dorsal roots. (that took me an hour to expose sacral and > lumber segments). > > If I count from conus medullaris, how can I be sure my counting > is correct? should I count main nerves or emerging > branches? Is there any hallmarks that can differentiate L6 from S1? > > According to what I see in mouse spinal cord, the last thick > ventral spinal nerve coming out from the spinal cord appears to > be several branches emerging from the cord then merging into > one, but then split into 2 again. One of those nerves > attach another dorsal root and DRG, yet I fail to preserve the > other DRG if any. As for the 2 ventral nerves below this > one are much thinner and also has few emerging branches from the > cord. So, I am not sure about what I see is L6 or it's a > merge of L6 & S1?? > > If I count the afforementioned nerve as L6, I can locate 3 pairs > of long dorsal and ventral roots (L6-L3) and 2 pairs of > relatively short L2-L1 above. Yet, L1 roots are notably longer > than Thoracic root along rib cage level. So, Is L1 always longer > than T12?? > > I read some histonet achieve suggesting saline injection to push > the spinal cord out, can the DRG be preserved? > > Thank you. > > Karen Ling > University of Southern California > Program in Neuroscience > 3641 Watt Way, HNB 209 > Los Angeles, CA 90089-2520 From talulahgosh <@t> gmail.com Fri Aug 10 23:57:17 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Aug 10 23:57:22 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: References: Message-ID: The best atlas I have heard of dealing with the mouse spinal cord is called "The House Mouse." It is no longer being published but the amazon link is here tinyuRL.com/2rnvul You may want to see if it's been updated with a more recent name. If not, try to find a used copy with bookfinder.com I haven't personally used this, but my boss swears by it, and she's an old school anatomist of mice and chicks. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. From ryan <@t> upei.ca Sat Aug 11 12:16:32 2007 From: ryan <@t> upei.ca (ryan@upei.ca) Date: Sat Aug 11 12:19:36 2007 Subject: [Histonet] Histonet Digest, Vol 45, Issue 16 Message-ID: <902667B197E@acad1.cs.upei.ca> C.L. Ryan will be out of the office from Friday Aug 10 until Monday Aug. 13, incl. From M.Goodyear <@t> latrobe.edu.au Mon Aug 13 02:27:50 2007 From: M.Goodyear <@t> latrobe.edu.au (Melinda Goodyear) Date: Mon Aug 13 02:28:57 2007 Subject: [Histonet] Starfrost slides IHC Message-ID: <61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8@EXCHANGE.ltu.edu.au> Hi all, I have been in contact with a third party from Australia that distributes Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH (Knittel Glaser) as my tissue sections have been falling off during immunohistochemistry using a new batch of slides (Starfrost Adhesive hydrophilic slides) with the same catalogue number as previously ordered. I received an explanation that all previous batches of slides sent may have been mis-labelled and probably had an adhesive applied like silane coated or poly-L-lysine. The only difference between the old batch of slides and new batch is that the old ones have 2 stars printed at the top of the slides and the boxes are labelled as "adhesive-Objekttrager" rather than "Objekttrager". I am wondering whether anybody else may be have been having problems with these slides and whether changing to silane coated may help? Thanks in advance for your help, Melinda Visual Neuroscience Lab School of Psychological Science Faculty of Science, Technology and Engineering La Trobe University Victoria, 3086 PH: +61 3 9479 2470 From hodges420 <@t> msn.com Mon Aug 13 06:40:01 2007 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Mon Aug 13 06:40:17 2007 Subject: [Histonet] (no subject) Message-ID: Has anyone had any trouble confirming their regristry from ASCP? I have completely dropped out of the system and don't know what or who to contact. Tere Hodges Tucson, Az _________________________________________________________________ [1]Booking a flight? Know when to buy with airfare predictions on MSN Travel. References 1. http://g.msn.com/8HMAENUS/2755??PS=47575 From mcauliff <@t> umdnj.edu Mon Aug 13 08:28:22 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Aug 13 08:29:49 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: References: Message-ID: <46C05C76.9020408@umdnj.edu> Greetings all: The link Emily supplied does not work and I could not find "The House Mouse" at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green (former director of the Jackson Lab) might have the information the original poster was seeking. Geoff Emily Sours wrote: > The best atlas I have heard of dealing with the mouse spinal cord is > called "The House Mouse." It is no longer being published but the > amazon link is here > tinyuRL.com/2rnvul > You may want to see if it's been updated with a more recent name. If > not, try to find a used copy with bookfinder.com > I haven't personally used this, but my boss swears by it, and she's an > old school anatomist of mice and chicks. > > Emily > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From talulahgosh <@t> gmail.com Mon Aug 13 08:42:13 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Aug 13 08:42:22 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: <46C05C76.9020408@umdnj.edu> References: <46C05C76.9020408@umdnj.edu> Message-ID: Here's a long link for The House Mouse at amazon http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059407/ref=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2 here's another try for the short one: http://www.tinyurl.com/2rnvul Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. From relia1 <@t> earthlink.net Mon Aug 13 09:41:13 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Aug 13 09:45:28 2007 Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors and Lead Techs Message-ID: Hello Histonetters, I have several exciting opportunities for experienced Histology Managers, Supervisors and Lead Technicians in hospital and private lab environments in several locations nationwide. The compensation packages are excellent, the work is challenging and the sky is the limit. The positions are of course full time and day shift. Here are my leadership positions: Histology Manager ? Central CA, Near Santa Barbara Histology Supervisor ? Gulf Coast, Texas Histology Supervisor ? Southeastern MA Histology Manager ? Chicago, IL If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Kristopher.Kalleberg <@t> unilever.com Mon Aug 13 10:15:53 2007 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Mon Aug 13 10:16:05 2007 Subject: [Histonet] Xylene substitutes Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76@NTRSEVS30002.s3.ms.unilever.com> Is anyone out there familiar with Xylene substitutes such as Histo-Clear? I am wondering if switching over the the substitutes is a good idea. Any positive or negative feedback would be greatly appreciated when it comes to IHC results. Thanks in adavance. Kris From mcauliff <@t> umdnj.edu Mon Aug 13 10:14:55 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Aug 13 10:16:24 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: References: Message-ID: <46C0756F.6000507@umdnj.edu> You might have a look at Progress in Brain Research 11:1-56, 1964 by Nieuwenhuys, a long paper on comparative anatomy of the spinal cord. Geoff Kar Ling wrote: > Hi, > > I have problems in defining ventral roots of lumbar spinal segments in mice. I try to look up some mouse anatomy book, but it only has bunch of spinal cord cross section. For gross anatomy of the spinal cord, I can only find rat ones. I wonder if there's such book with whole spinal cord illustration. > > My first questions is: Do mice has L1-L6 and S1-S4 as in rat? > > My way to dissect spinal cord is cutting the dorsal bones piece by pieces, and then carefully remove the ventral bones starting at the sides so that I can preserve DRG attached to both ventral and dorsal roots. (that took me an hour to expose sacral and lumber segments). > > If I count from conus medullaris, how can I be sure my counting is correct? should I count main nerves or emerging branches? Is there any hallmarks that can differentiate L6 from S1? > > According to what I see in mouse spinal cord, the last thick ventral spinal nerve coming out from the spinal cord appears to be several branches emerging from the cord then merging into one, but then split into 2 again. One of those nerves attach another dorsal root and DRG, yet I fail to preserve the other DRG if any. As for the 2 ventral nerves below this one are much thinner and also has few emerging branches from the cord. So, I am not sure about what I see is L6 or it's a merge of L6 & S1?? > > If I count the afforementioned nerve as L6, I can locate 3 pairs of long dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above. Yet, L1 roots are notably longer than Thoracic root along rib cage level. So, Is L1 always longer than T12?? > > I read some histonet achieve suggesting saline injection to push the spinal cord out, can the DRG be preserved? > > Thank you. > > Karen Ling > University of Southern California > Program in Neuroscience > 3641 Watt Way, HNB 209 > Los Angeles, CA 90089-2520 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Mon Aug 13 10:18:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 13 10:18:38 2007 Subject: [Histonet] Xylene substitutes In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <491845.24283.qm@web61216.mail.yahoo.com> Kristopher: This very same subject has been subject of discussion for years in Histonet. You are better off by consulting Histonet Archieves than waiting for direct answers. Ren? J. "Kalleberg, Kristopher" wrote: Is anyone out there familiar with Xylene substitutes such as Histo-Clear? I am wondering if switching over the the substitutes is a good idea. Any positive or negative feedback would be greatly appreciated when it comes to IHC results. Thanks in adavance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. From MICHELLE.MCNEESE <@t> childrens.com Mon Aug 13 10:35:40 2007 From: MICHELLE.MCNEESE <@t> childrens.com (MICHELLE MCNEESE) Date: Mon Aug 13 10:36:11 2007 Subject: [Histonet] Pre-filled containers of B-5 fixative Message-ID: <46C033FC020000290000A652@CNET3.CHILDRENS.COM> We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions: 1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5? 2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others. Thanks Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com From JWEEMS <@t> sjha.org Mon Aug 13 10:39:50 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Aug 13 10:40:18 2007 Subject: [Histonet] Pre-filled containers of B-5 fixative In-Reply-To: <46C033FC020000290000A652@CNET3.CHILDRENS.COM> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA11B@sjhaexc02.sjha.org> We use B-Plus fixative from BBC Biochemical. There will never be a fixative as good as mercury, but we must move forward to a better environment or all become mad hatters. Our pathologists took it kicking and screaming, but they have adjusted now. The hardest thing is to pull a previous bone marrow case to compare and the difference is so drastic. But it is a matter of retraining and accepting the change. Good luck! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of MICHELLE MCNEESE Sent: Monday, August 13, 2007 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-filled containers of B-5 fixative We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions: 1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5? 2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others. Thanks Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From doug <@t> ppspath.com Mon Aug 13 11:48:18 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Aug 13 10:49:52 2007 Subject: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative In-Reply-To: <46C033FC020000290000A652@CNET3.CHILDRENS.COM> Message-ID: Michelle, It is a simple switch. We use B-Plus from BBC biochemical. You can get the 20 containers. http://www.bbcus.com/products.html?pc=3&pid=58 Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE MCNEESE Sent: Monday, August 13, 2007 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions: 1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5? 2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others. Thanks Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Mon Aug 13 11:07:42 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Aug 13 11:07:54 2007 Subject: [Histonet] Looking for book Message-ID: I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From Jackie.O'Connor <@t> abbott.com Mon Aug 13 11:18:02 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Aug 13 11:18:50 2007 Subject: [Histonet] Looking for book In-Reply-To: Message-ID: You can get it through Amazon.com ISBN 1-881299-43-0. I just looked and it's available. JO'C "Perry, Margaret" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/13/2007 11:07 AM To cc Subject [Histonet] Looking for book I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Aug 13 11:19:42 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Aug 13 11:20:28 2007 Subject: [Histonet] Looking for book In-Reply-To: Message-ID: Nevermind. I was wrong. It's not available. Sorry. Stupid Monday alert. "Perry, Margaret" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/13/2007 11:07 AM To cc Subject [Histonet] Looking for book I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> TriadHospitals.com Mon Aug 13 12:06:13 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Mon Aug 13 12:06:39 2007 Subject: [Histonet] Alcian yellow Message-ID: <518A08C53ED96D419F498D309E64A36A1B6F67@CPRTEVS03.triadhospitals.net> Hi Everyone, I just looked at Polyscientific's web-site and saw that they are offering Alcian yellow as a 1% solution .... Is the Alcian Yellow (specifically) dye available from any vendor at this point? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com From ryan <@t> upei.ca Mon Aug 13 12:17:50 2007 From: ryan <@t> upei.ca (ryan@upei.ca) Date: Mon Aug 13 12:21:19 2007 Subject: [Histonet] Histonet Digest, Vol 45, Issue 18 Message-ID: <9326F5F3E7F@acad1.cs.upei.ca> C.L. Ryan will be out of the office from Friday Aug 10 until Monday Aug. 13, incl. From gcallis <@t> montana.edu Mon Aug 13 12:33:48 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Aug 13 12:33:54 2007 Subject: [Histonet] Looking for book In-Reply-To: References: Message-ID: <6.0.0.22.1.20070813113246.01bacbe8@gemini.msu.montana.edu> Eaton Publishing or go to the BioTechniques website. You can purchase it from there. At 10:07 AM 8/13/2007, you wrote: >I am looking for the book Antigen Retrieval Techniques: >Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang >Gu, Clive R. Taylor. > >This book is not available in bookstores I've searched on the Web and >I,ve tried Google and it is not available there either. Would anyone >have a copy that I can purchase? I am studying for my QIHC >certification. > > > > > >Margaret Perry HT (ASCP) > >IHC Lab Manager Veterinary Science > >Animal Disease Research and Diagnostic Lab > >South Dakota State University > >Box 2175 North Campus Drive > >Brookings SD 57007 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From LRaff <@t> lab.uropartners.com Mon Aug 13 12:33:49 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Mon Aug 13 12:34:02 2007 Subject: [Histonet] FISHing trip Message-ID: <5DA1CA5D0B98A84985B545A24423B822053085@UPLAB01.uplab.local> I haven't gotten any help from the pathologist listserve, and the UroVysion FISH listserve doesn't have many users, so I am asking the histotechs for help! Maybe you have friends doing FISH.... We are doing FISH studies on urine (UroVysion) and frequently run into cell clumps/clusters. Since the architecture is not what we are assessing via FISH, it would be useful to disrupt these clumps and obtain single cells for analysis. Does anyone have a protocol for doing this on urine specimens? Thanks in advance. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From pruegg <@t> ihctech.net Mon Aug 13 13:21:07 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Mon Aug 13 13:18:22 2007 Subject: [Histonet] Looking for book In-Reply-To: <6.0.0.22.1.20070813113246.01bacbe8@gemini.msu.montana.edu> Message-ID: <200708131817.l7DIHw7r004734@pro12.abac.com> Margaret, I think I purchased mine less than 2 years ago on Amazon.com Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, August 13, 2007 11:34 AM To: Perry, Margaret; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for book Eaton Publishing or go to the BioTechniques website. You can purchase it from there. At 10:07 AM 8/13/2007, you wrote: >I am looking for the book Antigen Retrieval Techniques: >Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang >Gu, Clive R. Taylor. > >This book is not available in bookstores I've searched on the Web and >I,ve tried Google and it is not available there either. Would anyone >have a copy that I can purchase? I am studying for my QIHC >certification. > > > > > >Margaret Perry HT (ASCP) > >IHC Lab Manager Veterinary Science > >Animal Disease Research and Diagnostic Lab > >South Dakota State University > >Box 2175 North Campus Drive > >Brookings SD 57007 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Aug 13 13:20:10 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Aug 13 13:19:33 2007 Subject: [Histonet] Alcian yellow In-Reply-To: <518A08C53ED96D419F498D309E64A36A1B6F67@CPRTEVS03.triadhospitals.net> References: <518A08C53ED96D419F498D309E64A36A1B6F67@CPRTEVS03.triadhospitals.net> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4BA3@bruexchange1.digestivespecialists.com> Robert, I haven't found any Alcian Yellow powder. I did find a company that said they had it and I ordered it. (with a credit card) After contacting them every couple of weeks for six months and being told it would be two more weeks I canceled the order. I have been using HP Yellow from Anatech for a year now and have found it (with a little tweaking) to be quite good. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Monday, August 13, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian yellow Hi Everyone, I just looked at Polyscientific's web-site and saw that they are offering Alcian yellow as a 1% solution .... Is the Alcian Yellow (specifically) dye available from any vendor at this point? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Aug 13 13:39:09 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Mon Aug 13 13:36:22 2007 Subject: [Histonet] htl's In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB72A8@EMAIL.archildrens.org> Message-ID: <200708131836.l7DIa0Ti015479@pro12.abac.com> I believe that they would as they were grandfathered as if they had the BS, only those who applied during a short period of time after the HTL was offered were grandfathered in without a BS degree, but I would think that the required amount of experience counted as if you had the degree and now that one is called an HTL they would qualify as if they had a BS degree????? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, August 09, 2007 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] htl's Importance: High Is a person with an HTL certification considered 'high complexity testing personnel' even if they do not have a BS degree? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Mon Aug 13 13:58:18 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Aug 13 13:58:46 2007 Subject: [Histonet] FISHing trip In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822053085@UPLAB01.uplab.local> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6DDB@NVCIEXCH02.NVCI.org> I don't have a protocol for doing this on urine, but if I wanted to do it, the first thing I'd try would be trypsanizing the cells after spinning to obtain a pellet. Then I'd spin down again, decant the remaining trypsan, re-suspend in PBS, and make cytospins. Then it's happy FISHing! Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff Sent: Monday, August 13, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISHing trip I haven't gotten any help from the pathologist listserve, and the UroVysion FISH listserve doesn't have many users, so I am asking the histotechs for help! Maybe you have friends doing FISH.... We are doing FISH studies on urine (UroVysion) and frequently run into cell clumps/clusters. Since the architecture is not what we are assessing via FISH, it would be useful to disrupt these clumps and obtain single cells for analysis. Does anyone have a protocol for doing this on urine specimens? Thanks in advance. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From sbanwait <@t> buckinstitute.org Mon Aug 13 14:04:30 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Mon Aug 13 14:04:42 2007 Subject: [Histonet] paraffin embedding mouse eyes Message-ID: Hi There, Can mouse eyes undergo tissue processing for paraffin embedding? Does anyone have a protocol, (is it different from other tissues? ) ? Thanks, Surita ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate III Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From ElizabethWyand <@t> texashealth.org Mon Aug 13 14:06:53 2007 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Mon Aug 13 14:07:25 2007 Subject: [Histonet] unsubscribe Message-ID: <26BE9ACC202D29479B0A144066A733E50276DE5E@phdex01.txhealth.org> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Charles.Embrey <@t> carle.com Mon Aug 13 15:10:05 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Aug 13 15:10:18 2007 Subject: [Histonet] htl's In-Reply-To: <200708131836.l7DIa0Ti015479@pro12.abac.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE521@EXCHANGEBE1.carle.com> If, and only if, they were documented doing High Complexity Testing on or prior to 24 April 1995. Grandfathering for CLIA '88 and ASCP are two separate things. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy ruegg Sent: Monday, August 13, 2007 1:39 PM To: 'Horn, Hazel V'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] htl's I believe that they would as they were grandfathered as if they had the BS, only those who applied during a short period of time after the HTL was offered were grandfathered in without a BS degree, but I would think that the required amount of experience counted as if you had the degree and now that one is called an HTL they would qualify as if they had a BS degree????? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, August 09, 2007 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] htl's Importance: High Is a person with an HTL certification considered 'high complexity testing personnel' even if they do not have a BS degree? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------ ---- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Aug 13 15:32:12 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 13 15:32:33 2007 Subject: [Histonet] htl's In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE521@EXCHANGEBE1.carle.com> Message-ID: <005e01c7dde9$0abee6b0$d00f7ca5@lurie.northwestern.edu> I got my BS in 1984 and am an HTL. Those that were grandfathered in did not have to have a BS- it was from experience. I know of two people that were grandfathered in,one of whom helped create the HTL designation. Believe it or not she is now an interior designer! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Monday, August 13, 2007 3:10 PM To: patsy ruegg; Horn, Hazel V; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] htl's If, and only if, they were documented doing High Complexity Testing on or prior to 24 April 1995. Grandfathering for CLIA '88 and ASCP are two separate things. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy ruegg Sent: Monday, August 13, 2007 1:39 PM To: 'Horn, Hazel V'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] htl's I believe that they would as they were grandfathered as if they had the BS, only those who applied during a short period of time after the HTL was offered were grandfathered in without a BS degree, but I would think that the required amount of experience counted as if you had the degree and now that one is called an HTL they would qualify as if they had a BS degree????? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, August 09, 2007 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] htl's Importance: High Is a person with an HTL certification considered 'high complexity testing personnel' even if they do not have a BS degree? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------ ---- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From agustinvictor <@t> gmail.com Mon Aug 13 15:47:37 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Mon Aug 13 15:47:48 2007 Subject: [Histonet] Thiocarbohydrazide Message-ID: <015b01c7ddeb$323d4e00$590215ac@Pentium4> Hi Histoneters! I need a protocole for OTO Technics (Osmium tetroxide, Thiocarbohydrazide)specially as preparing the hidrocarbothyazide solution and the correct temperature Thanks in advanced ! Ht Agustin Victor Chertcoff Electron Microscopy Service National Institute of Microbiology C G Malbran Buenos Aires Argentina From AFoshey <@t> chw.org Mon Aug 13 15:58:57 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Mon Aug 13 15:59:10 2007 Subject: [Histonet] Labeling of control slides for Special stains and IHC Message-ID: <9E6D52F532809247BDA1783680E92C560B702861@CHWEXC.chwi.chswi.org> We have had some discussion on how to label the control slides recently and I would like to get input from other labs on how they label the controls and how they identify where the controls are filed. We currently use Powerpath (v 8.3) labels to label our stains which include the pathology number, the name of the stain, the patient name and the institution. The control slides are labeled QC: followed by the name of stain and we would write the case number on the slide label and when the slide is filed it is filed with the case. When multiple patient slides for the same stain were stained the positive control was listed on the patient slide on the bottom of the label indicating the case number of the control. . The pathologists are changing the labeling to the date only on the control slide and the batch number. Any suggestions would be appreciated. Thanks, Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From rjbuesa <@t> yahoo.com Mon Aug 13 16:12:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 13 16:12:45 2007 Subject: [Histonet] Labeling of control slides for Special stains and IHC In-Reply-To: <9E6D52F532809247BDA1783680E92C560B702861@CHWEXC.chwi.chswi.org> Message-ID: <186611.47634.qm@web61222.mail.yahoo.com> Annette: Sound somewhat complicated. We used to do as follows: 1-single cases with single control: both with the case (both with case number); 2-multiple cases with single control: these type of controls in a separate file with the date the test for the multiple cases were done. Ren? J. "Foshey, Annette" wrote: We have had some discussion on how to label the control slides recently and I would like to get input from other labs on how they label the controls and how they identify where the controls are filed. We currently use Powerpath (v 8.3) labels to label our stains which include the pathology number, the name of the stain, the patient name and the institution. The control slides are labeled QC: followed by the name of stain and we would write the case number on the slide label and when the slide is filed it is filed with the case. When multiple patient slides for the same stain were stained the positive control was listed on the patient slide on the bottom of the label indicating the case number of the control. . The pathologists are changing the labeling to the date only on the control slide and the batch number. Any suggestions would be appreciated. Thanks, Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From david.kinsley <@t> spcorp.com Mon Aug 13 17:05:26 2007 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Mon Aug 13 17:05:31 2007 Subject: [Histonet] Kaiser's solution Message-ID: Hi, Does anyone know where I may be able to purchase Kaiser's solution for cover slipping, or does anyone have a protocol for preparing it? thanks Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From rjbuesa <@t> yahoo.com Mon Aug 13 17:15:51 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 13 17:15:56 2007 Subject: [Histonet] Kaiser's solution In-Reply-To: Message-ID: <243116.15560.qm@web61224.mail.yahoo.com> David: I imagine you are referring to glycerol jelly. There are two formulas:one from Kaiser (1880): dist. water (40 mL) + gelatin (7 g) + glycerol (50 mL) + phenol (1 g), and another by Kisser (1935): dist. water (60 mL) + glyverol (50 mL) + gelatin (16 g) + phenol (1 g) Ren? J. "Kinsley, David" wrote: Hi, Does anyone know where I may be able to purchase Kaiser's solution for cover slipping, or does anyone have a protocol for preparing it? thanks Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From MMINOKENUDSON <@t> PARTNERS.ORG Mon Aug 13 18:34:54 2007 From: MMINOKENUDSON <@t> PARTNERS.ORG (Mino-Kenudson, Mari,M.D.) Date: Mon Aug 13 18:35:01 2007 Subject: [Histonet] RE: bmp4 Message-ID: Hello, I am looking for a bmp4 antibody which works for IHC on paraffin embedded tissue. If anybody has experience with bmp4 and give me any suggestions, I would really appreciate it. Best, Mari The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From JWEEMS <@t> sjha.org Mon Aug 13 19:29:45 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Aug 13 19:30:02 2007 Subject: [Histonet] Labeling of control slides for Special stains and IHC In-Reply-To: <186611.47634.qm@web61222.mail.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA12E@sjhaexc02.sjha.org> And we date the controls and file them by date. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, August 13, 2007 5:13 PM To: Foshey, Annette; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Labeling of control slides for Special stains and IHC Annette: Sound somewhat complicated. We used to do as follows: 1-single cases with single control: both with the case (both with case number); 2-multiple cases with single control: these type of controls in a separate file with the date the test for the multiple cases were done. Ren? J. "Foshey, Annette" wrote: We have had some discussion on how to label the control slides recently and I would like to get input from other labs on how they label the controls and how they identify where the controls are filed. We currently use Powerpath (v 8.3) labels to label our stains which include the pathology number, the name of the stain, the patient name and the institution. The control slides are labeled QC: followed by the name of stain and we would write the case number on the slide label and when the slide is filed it is filed with the case. When multiple patient slides for the same stain were stained the positive control was listed on the patient slide on the bottom of the label indicating the case number of the control. . The pathologists are changing the labeling to the date only on the control slide and the batch number. Any suggestions would be appreciated. Thanks, Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ree3 <@t> leicester.ac.uk Tue Aug 14 03:16:41 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Aug 14 03:17:17 2007 Subject: [Histonet] Kaiser's solution In-Reply-To: References: Message-ID: I thought that the "Kaiser's Solution" was European, if not world domination!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kinsley, David Sent: 13 August 2007 23:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kaiser's solution Hi, Does anyone know where I may be able to purchase Kaiser's solution for cover slipping, or does anyone have a protocol for preparing it? thanks Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Tue Aug 14 07:09:41 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Aug 14 07:09:56 2007 Subject: [Histonet] Looking for book Message-ID: <81196.87505.qm@web50301.mail.re2.yahoo.com> Margaret, I tried to order this from Amazon last year and after 6 months of them resetting the delivery date, they finally said it was unavailable. I am still trying to find this book. I haev done extensive google searches and the book always comes up as unavailable. This morning, I looked on Biotechniques as Gayle suggested, but I can't seem to find it. I will probably call the publisher today. Anyway, I have decided to study for the QIHC test without this book, it seems like a lost cause. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "Perry, Margaret" To: histonet@lists.utsouthwestern.edu Sent: Monday, August 13, 2007 12:07:42 PM Subject: [Histonet] Looking for book I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/ From mcauliff <@t> umdnj.edu Tue Aug 14 08:50:25 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Aug 14 08:51:12 2007 Subject: [Histonet] Kaiser's solution In-Reply-To: <243116.15560.qm@web61224.mail.yahoo.com> References: <243116.15560.qm@web61224.mail.yahoo.com> Message-ID: <46C1B321.5000302@umdnj.edu> John Kiernan's website has several excellent recipes for glycerine jelly. Geoff Rene J Buesa wrote: > David: > I imagine you are referring to glycerol jelly. > There are two formulas:one from Kaiser (1880): > dist. water (40 mL) + gelatin (7 g) + glycerol (50 mL) + phenol (1 g), > and another by Kisser (1935): > dist. water (60 mL) + glyverol (50 mL) + gelatin (16 g) + phenol (1 g) > Ren? J. > > "Kinsley, David" wrote: > Hi, > > Does anyone know where I may be able to purchase Kaiser's solution for > cover slipping, or does anyone have a protocol for preparing it? > > thanks > > Dave > ********************************************************************* > This message and any attachments are solely for the > intended recipient. If you are not the intended recipient, > disclosure, copying, use or distribution of the information > included in this message is prohibited -- Please > immediately and permanently delete. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Building a website is a piece of cake. > Yahoo! Small Business gives you all the tools to get online. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Tbarnhart <@t> primecare.org Tue Aug 14 09:07:37 2007 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Tue Aug 14 09:06:16 2007 Subject: [Histonet] H-pylori controls Message-ID: <4F0B7161A6CD524FAD8017D52E1553407AE51D@exchangent> Anybody out there in histoland have extra H-pylori controls they would like to barter? I have many, many other tissues and controls that we can swap. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Manager St. Alexius Medical Center Bismarck, ND From cbass <@t> bidmc.harvard.edu Tue Aug 14 09:16:53 2007 From: cbass <@t> bidmc.harvard.edu (cbass@bidmc.harvard.edu) Date: Tue Aug 14 09:17:15 2007 Subject: [Histonet] beta-tubulin staining of neurites Message-ID: <4B5700BE37215344A81185F131A848C261EB83@EVS6.its.caregroup.org> Hello Everyone, I'm looking for a decent protocol for staining beta-tubulin in tissue culture. Basically, we are doing a neurite extension assay in chamber wells, where the neurites extend onto the filter in culture and then we stain the neurites for beta-tubulin. We use the following reagents: SHS-Y5Y cells beta-tubulin antibody, rabbit polyclonal, Cell Signaling, Cat #2146 Alexafluor 488, goat anti-rabbit, invitrogen Cat # A11008 We are new to immunofluorescence so any help would be appreciated. We are trying this out initially in cells plated in regular 24-well plates. The best we've seen is a light diffuse staining with 1:500 of the primary and 1:2000 of the secondary. My guess is that both dilutions have to be cut down. Any suggestions? Also, should there be a permeabilization step? I think we tried tween-20 in one step. Do we need triton-x? Thanks everyone! Caroline From danma <@t> reg2.health.nb.ca Tue Aug 14 09:19:06 2007 From: danma <@t> reg2.health.nb.ca (Danahy, Mary-Lee (R2)) Date: Tue Aug 14 09:19:27 2007 Subject: [Histonet] RE: Histonet Digest, Vol 45, Issue 18 In-Reply-To: <64sr41$dgr5a@Ironport2.rha-rrs.ca> Message-ID: <414FA20EC03C3140924A19ACF103D2974C1315@RHAEX2.RHA-RRS.CA> Help...again, Does anybody have any info. on calectin-3 ? What is it? Who uses it? Where do you get it? Does anybody else use the Breast Receptor Block from Zymed ? If so how do you find it works for you? Thanks for listening. Mary Lee Danahy R.T.,M.L.T.III Immunohistochemistry/Molecular Lab. Pathology Dept. Atlantic Health Sciences Corporation Saint John N.B.Canada tel.# 506-648-6604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, August 13, 2007 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 45, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Starfrost slides IHC (Melinda Goodyear) 2. (no subject) (MARY T HODGES) 3. Re: mouse spinal cord atlas (Geoff McAuliffe) 4. Re: mouse spinal cord atlas (Emily Sours) 5. RELIA Special Job Alert for Managers, Supervisors and Lead Techs (Pam Barker) 6. Xylene substitutes (Kalleberg, Kristopher) 7. Re: mouse spinal cord atlas (Geoff McAuliffe) 8. Re: Xylene substitutes (Rene J Buesa) 9. Pre-filled containers of B-5 fixative (MICHELLE MCNEESE) 10. RE: Pre-filled containers of B-5 fixative (Weems, Joyce) 11. RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative (Douglas D Deltour) 12. Looking for book (Perry, Margaret) 13. Re: Looking for book (Jackie M O'Connor) 14. Re: Looking for book (Jackie M O'Connor) ---------------------------------------------------------------------- Message: 1 Date: Mon, 13 Aug 2007 17:27:50 +1000 From: "Melinda Goodyear" Subject: [Histonet] Starfrost slides IHC To: Message-ID: <61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8@EXCHANGE.ltu.edu.au> Content-Type: text/plain; charset="us-ascii" Hi all, I have been in contact with a third party from Australia that distributes Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH (Knittel Glaser) as my tissue sections have been falling off during immunohistochemistry using a new batch of slides (Starfrost Adhesive hydrophilic slides) with the same catalogue number as previously ordered. I received an explanation that all previous batches of slides sent may have been mis-labelled and probably had an adhesive applied like silane coated or poly-L-lysine. The only difference between the old batch of slides and new batch is that the old ones have 2 stars printed at the top of the slides and the boxes are labelled as "adhesive-Objekttrager" rather than "Objekttrager". I am wondering whether anybody else may be have been having problems with these slides and whether changing to silane coated may help? Thanks in advance for your help, Melinda Visual Neuroscience Lab School of Psychological Science Faculty of Science, Technology and Engineering La Trobe University Victoria, 3086 PH: +61 3 9479 2470 ------------------------------ Message: 2 Date: Mon, 13 Aug 2007 07:40:01 -0400 From: "MARY T HODGES" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" Has anyone had any trouble confirming their regristry from ASCP? I have completely dropped out of the system and don't know what or who to contact. Tere Hodges Tucson, Az _________________________________________________________________ [1]Booking a flight? Know when to buy with airfare predictions on MSN Travel. References 1. http://g.msn.com/8HMAENUS/2755??PS=47575 ------------------------------ Message: 3 Date: Mon, 13 Aug 2007 09:28:22 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] mouse spinal cord atlas To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Message-ID: <46C05C76.9020408@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Greetings all: The link Emily supplied does not work and I could not find "The House Mouse" at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green (former director of the Jackson Lab) might have the information the original poster was seeking. Geoff Emily Sours wrote: > The best atlas I have heard of dealing with the mouse spinal cord is > called "The House Mouse." It is no longer being published but the > amazon link is here tinyuRL.com/2rnvul You may want to see if it's > been updated with a more recent name. If not, try to find a used copy > with bookfinder.com I haven't personally used this, but my boss swears > by it, and she's an old school anatomist of mice and chicks. > > Emily > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 4 Date: Mon, 13 Aug 2007 09:42:13 -0400 From: "Emily Sours" Subject: Re: [Histonet] mouse spinal cord atlas To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Here's a long link for The House Mouse at amazon http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059407/ref=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2 here's another try for the short one: http://www.tinyurl.com/2rnvul Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. ------------------------------ Message: 5 Date: Mon, 13 Aug 2007 10:41:13 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors and Lead Techs To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters, I have several exciting opportunities for experienced Histology Managers, Supervisors and Lead Technicians in hospital and private lab environments in several locations nationwide. The compensation packages are excellent, the work is challenging and the sky is the limit. The positions are of course full time and day shift. Here are my leadership positions: Histology Manager - Central CA, Near Santa Barbara Histology Supervisor - Gulf Coast, Texas Histology Supervisor - Southeastern MA Histology Manager - Chicago, IL If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net ------------------------------ Message: 6 Date: Mon, 13 Aug 2007 11:15:53 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] Xylene substitutes To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Is anyone out there familiar with Xylene substitutes such as Histo-Clear? I am wondering if switching over the the substitutes is a good idea. Any positive or negative feedback would be greatly appreciated when it comes to IHC results. Thanks in adavance. Kris ------------------------------ Message: 7 Date: Mon, 13 Aug 2007 11:14:55 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] mouse spinal cord atlas To: Kar Ling Cc: histonet@lists.utsouthwestern.edu Message-ID: <46C0756F.6000507@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 You might have a look at Progress in Brain Research 11:1-56, 1964 by Nieuwenhuys, a long paper on comparative anatomy of the spinal cord. Geoff Kar Ling wrote: > Hi, > > I have problems in defining ventral roots of lumbar spinal segments in mice. I try to look up some mouse anatomy book, but it only has bunch of spinal cord cross section. For gross anatomy of the spinal cord, I can only find rat ones. I wonder if there's such book with whole spinal cord illustration. > > My first questions is: Do mice has L1-L6 and S1-S4 as in rat? > > My way to dissect spinal cord is cutting the dorsal bones piece by pieces, and then carefully remove the ventral bones starting at the sides so that I can preserve DRG attached to both ventral and dorsal roots. (that took me an hour to expose sacral and lumber segments). > > If I count from conus medullaris, how can I be sure my counting is correct? should I count main nerves or emerging branches? Is there any hallmarks that can differentiate L6 from S1? > > According to what I see in mouse spinal cord, the last thick ventral spinal nerve coming out from the spinal cord appears to be several branches emerging from the cord then merging into one, but then split into 2 again. One of those nerves attach another dorsal root and DRG, yet I fail to preserve the other DRG if any. As for the 2 ventral nerves below this one are much thinner and also has few emerging branches from the cord. So, I am not sure about what I see is L6 or it's a merge of L6 & S1?? > > If I count the afforementioned nerve as L6, I can locate 3 pairs of long dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above. Yet, L1 roots are notably longer than Thoracic root along rib cage level. So, Is L1 always longer than T12?? > > I read some histonet achieve suggesting saline injection to push the spinal cord out, can the DRG be preserved? > > Thank you. > > Karen Ling > University of Southern California > Program in Neuroscience > 3641 Watt Way, HNB 209 > Los Angeles, CA 90089-2520 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 8 Date: Mon, 13 Aug 2007 08:18:22 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Xylene substitutes To: "Kalleberg, Kristopher" , histonet@lists.utsouthwestern.edu Message-ID: <491845.24283.qm@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Kristopher: This very same subject has been subject of discussion for years in Histonet. You are better off by consulting Histonet Archieves than waiting for direct answers. Ren? J. "Kalleberg, Kristopher" wrote: Is anyone out there familiar with Xylene substitutes such as Histo-Clear? I am wondering if switching over the the substitutes is a good idea. Any positive or negative feedback would be greatly appreciated when it comes to IHC results. Thanks in adavance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. ------------------------------ Message: 9 Date: Mon, 13 Aug 2007 10:35:40 -0500 From: "MICHELLE MCNEESE" Subject: [Histonet] Pre-filled containers of B-5 fixative To: Message-ID: <46C033FC020000290000A652@CNET3.CHILDRENS.COM> Content-Type: text/plain; charset=US-ASCII We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions: 1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5? 2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others. Thanks Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com ------------------------------ Message: 10 Date: Mon, 13 Aug 2007 11:39:50 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Pre-filled containers of B-5 fixative To: "MICHELLE MCNEESE" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA11B@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" We use B-Plus fixative from BBC Biochemical. There will never be a fixative as good as mercury, but we must move forward to a better environment or all become mad hatters. Our pathologists took it kicking and screaming, but they have adjusted now. The hardest thing is to pull a previous bone marrow case to compare and the difference is so drastic. But it is a matter of retraining and accepting the change. Good luck! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of MICHELLE MCNEESE Sent: Monday, August 13, 2007 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-filled containers of B-5 fixative We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions: 1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5? 2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others. Thanks Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 11 Date: Mon, 13 Aug 2007 11:48:18 -0500 From: "Douglas D Deltour" Subject: RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative To: "'MICHELLE MCNEESE'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Michelle, It is a simple switch. We use B-Plus from BBC biochemical. You can get the 20 containers. http://www.bbcus.com/products.html?pc=3&pid=58 Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE MCNEESE Sent: Monday, August 13, 2007 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative We are in the process of eliminating B-5 fixative from our facility; however, it is not a quick change or easy change for us and our supplier of the small pre-filled containers (Poly Scientific) will no longer fill orders after October. While we have eliminated the bulk of our use, we do still have a few specific biopsies for which we are trying to find a suitable alternative. With that said, I have two questions: 1. Is there a place where I can still get small (20 ml) pre-filled containers of B-5? 2. What are some of the alternatives that people use for kidney, liver, bone marrow, and the occassional lymph node? We have managed to move our heart and GI biopsies, but still need the others. Thanks Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 13 Aug 2007 11:07:42 -0500 From: "Perry, Margaret" Subject: [Histonet] Looking for book To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ------------------------------ Message: 13 Date: Mon, 13 Aug 2007 11:18:02 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] Looking for book To: "Perry, Margaret" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" You can get it through Amazon.com ISBN 1-881299-43-0. I just looked and it's available. JO'C "Perry, Margaret" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/13/2007 11:07 AM To cc Subject [Histonet] Looking for book I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 13 Aug 2007 11:19:42 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] Looking for book To: "Perry, Margaret" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Nevermind. I was wrong. It's not available. Sorry. Stupid Monday alert. "Perry, Margaret" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/13/2007 11:07 AM To cc Subject [Histonet] Looking for book I am looking for the book Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology by: Shan-Rong Shi, Jiang Gu, Clive R. Taylor. This book is not available in bookstores I've searched on the Web and I,ve tried Google and it is not available there either. Would anyone have a copy that I can purchase? I am studying for my QIHC certification. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 45, Issue 18 **************************************** -------------R2 DISCLAIMER------------- This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any action related with this communication as well as any reproduction, transmission and/or dissemination in whole or in part is strictly prohibited. If you have received this communication in error please notify the sender and delete this email immediately. Thank you. Ce message ?lectronique et tout fichier qui y est joint pourraient contenir des renseignements privil?gi?s ou confidentiels destin?s uniquement ? la personne ou ? l'organisme nomm? ci-dessus. Toute action entreprise relativement ? ce message ainsi que toute reproduction, transmission ou diffusion partielle ou totale de celui-ci sont strictement d?fendues. Si vous avez re?u ce message ?lectronique par erreur, veuillez en informer l'exp?diteur et d?truire le message imm?diatement. Merci. From algranth <@t> u.arizona.edu Tue Aug 14 09:29:15 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Aug 14 09:34:14 2007 Subject: [Histonet] Alcian yellow In-Reply-To: <518A08C53ED96D419F498D309E64A36A1B6F67@CPRTEVS03.triadhosp itals.net> References: <518A08C53ED96D419F498D309E64A36A1B6F67@CPRTEVS03.triadhospitals.net> Message-ID: <6.2.3.4.1.20070814072746.01f39a98@algranth.inbox.email.arizona.edu> I just got a card from Newcomer advertising Alcian Yellow in a 1% solution as part of a helicobactor stain kit. Andi At 10:06 AM 8/13/2007, Lott, Robert wrote: > > >Hi Everyone, > >I just looked at Polyscientific's web-site and saw that they are >offering Alcian yellow as a 1% solution .... > >Is the Alcian Yellow (specifically) dye available from any vendor at >this point? > > > >Robert > > > >Robert L. Lott, HTL(ASCP) > >Manager, Anatomic Pathology > >Trinity Medical Center / LabFirst > >800 Montclair Road > >Birmingham, AL 35213 > >205-592-5388 > >205-592-5646 - fax > >robert.lott@triadhospitals.com > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From asachau <@t> titanmed.com Tue Aug 14 09:33:40 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Tue Aug 14 09:35:34 2007 Subject: [Histonet] Position Available In-Reply-To: <414FA20EC03C3140924A19ACF103D2974C1315@RHAEX2.RHA-RRS.CA> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7B57EBB@titansbs1.corp.titanmed.com> Hello Histo-netters! I have a position available in a tropical vacation destination! Who wouldn't want to get off work and head to the beach to relax...? This position is a day shift regular Histology position. Candidates must have ASCP certification. Approximate start date of 9/5/07, 13 week assignment. Flight, Rental car and Housing (in a Resort... of course!) provided. Give me a call or email me ONLY if you are SERIOUSLY interested. This position will be filled this week. Thanks!!! :) April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From relia1 <@t> earthlink.net Tue Aug 14 09:35:38 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Aug 14 09:35:48 2007 Subject: [Histonet] RELIA Histology Job Alert - 8/14/07 Message-ID: Hi Histonetters, I hope everybody is having a great day. This is just a quick update on some of my newest positions. All of the positions that I work with are full time permanent positions with premier companies setting the mark to be ?employers of choice? in their respective areas. My clients offer competitive salaries excellent benefits and relocation assistance. New Grads and Experienced Techs are welcome to apply! Here are some of my newest openings: Histo Tech ? Ohio near Columbus Histo Tech ? Greater Los Angeles, CA Histo Tech ? St. Petersburg, FL Tech Lead/Supervisor - Corpus Christi, TX Histo Tech w/ IHC - Atlanta, GA Histo Tech ? Orlando, FL 3rd shift (2 positions dermpath lab) I also am recruiting for several great management positions: Histology Supervisor - Cape Cod, MA Histology Manager ? CA Santa Barbara area Histology Manager ? Chicago, IL Histology Supervisor - Corpus Christi, TX I also have openings in Texas, Pennsylvania, Washington State, Wisconsin, Massachusetts and California. For a complete listing of my current openings please go to www.jobvertise.com and search relia. If you are interested in any of these positions or would like to talk about jobs in other areas please shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. (I am available after hours) Also if you know of anyone else who might be interested please feel free to pass this information along to them as well. Have a great day!!! Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From mbmphoto <@t> gmail.com Tue Aug 14 09:58:30 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Aug 14 09:56:56 2007 Subject: [Histonet] Microm HM450 microtome Message-ID: <05921466-D60F-456B-8D7F-26FA65E48C21@gmail.com> Hello, I need to speak with anyone who uses or has used the Microm HM 450 sliding microtome. I particularly interested in the paraffin sectioning part. Knowing how a sliding microtome cuts, how is it possible to cut a paraffin ribbon using a sliding microtome. Please contact me at this email address: maria.mejia@ucsf.edu From vsnider <@t> shrinenet.org Tue Aug 14 10:17:18 2007 From: vsnider <@t> shrinenet.org (Snider, Vivian Deanna) Date: Tue Aug 14 10:19:18 2007 Subject: [Histonet] Mi Embedding Matrix Message-ID: <84BE46B37B314D409C5A17B7BAB022D60168F1C2@IDC-EX-VS01.shriners.cc> Hello netters, I am in need of your input once again! The researchers here use a product called MI Embedding Matrix, manufactured by Thermo Shandon, (or whatever name they use now) or have knowledge of it. The researchers here embed their own frozens and I cut them. Peridoically over the years we see "holes" where the nuclei should be. I have contacted Thermo Shandon for their tech support, and followed everything they suggested. I am at a loss. The tissue being embedded is in vitro skin substitute with human cells; very small and very thin, so I don't think this is a big issue. I cut at 4 - 5 microns at -20. The only thing affected is the nucleus. I am really puzzled and do not really know where to turn for answers. Any thoughts are appreciated. Thanks in advance, Deanna Snider HT ASCP Lead Technologist Shriners Hospital for Children CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From making <@t> ufl.edu Tue Aug 14 10:40:11 2007 From: making <@t> ufl.edu (MKing) Date: Tue Aug 14 10:43:27 2007 Subject: [Histonet] phenol substitute Message-ID: <46C1CCDB.2080203@ufl.edu> The recent posts on glycerol-gelatin mounting medium (Kaiser's solution) got me to wondering what the purpose of phenol is, and what might be a rational and less toxic substitute. Is is just antimicrobial? Mike King UF Pharmacology & Therapeutics From valeria.berno <@t> embl.it Tue Aug 14 10:48:39 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Tue Aug 14 10:48:52 2007 Subject: [Histonet] Ovary aromatase Message-ID: Hi!!! As most of the people posting a message here...I need help! One user is trying to detect aromatase by immunofluorescence in mouse ovary....problem: the autofluorescence on the cells around the follicle is too high and we still didn't get any specific staining or maybe the staining is weaker than the autofluorescence. The fact is that the autofluorescence is really high only in these cells and not all over the tissue! The tissue is frozen and PFA fixed. We also tried to use Far Red secondary antibody but with no luck. Any suggestion will be great Thanks Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.11.17/951 - Release Date: 13/08/2007 10.15 From rjbuesa <@t> yahoo.com Tue Aug 14 11:02:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 14 11:02:16 2007 Subject: [Histonet] phenol substitute In-Reply-To: <46C1CCDB.2080203@ufl.edu> Message-ID: <291662.56827.qm@web61218.mail.yahoo.com> If you "travel back in time" and realize that when Kaiser developed his formula it was 1880, on those years (after Lister) the "universal" antimicrobial was phenol (or "phenic acid"). The posting was about the formula composition, not about if phenol should be avoided TODAY or not. Ren? J. MKing wrote: The recent posts on glycerol-gelatin mounting medium (Kaiser's solution) got me to wondering what the purpose of phenol is, and what might be a rational and less toxic substitute. Is is just antimicrobial? Mike King UF Pharmacology & Therapeutics _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From Allison_Scott <@t> hchd.tmc.edu Tue Aug 14 11:08:45 2007 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Aug 14 11:08:58 2007 Subject: [Histonet] Frozen section turnaround time Message-ID: <1872B4A455B7974391609AD8034C79FC8BD383@LBEXCH01.hchd.local> Hello to everyone in histoland. I have a question concerning frozen section turnaround time. When you do your turnaround time report do you do it based on multiple sections or single sections. The bulk of our cases are multiple block frozens. They never meet the 20 min. time limit. The report that I turn in never meets the 100% compliance rate based on my numbers. Would someone be willing to share a report with me. My manager is concerned that we are not being compliant. Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From JWEEMS <@t> sjha.org Tue Aug 14 11:11:19 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Aug 14 11:11:52 2007 Subject: [Histonet] Frozen section turnaround time In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD383@LBEXCH01.hchd.local> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA14E@sjhaexc02.sjha.org> We count per block - if 24 min for a 3 blk frozen, it is 8 min each, etc. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: Tuesday, August 14, 2007 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section turnaround time Hello to everyone in histoland. I have a question concerning frozen section turnaround time. When you do your turnaround time report do you do it based on multiple sections or single sections. The bulk of our cases are multiple block frozens. They never meet the 20 min. time limit. The report that I turn in never meets the 100% compliance rate based on my numbers. Would someone be willing to share a report with me. My manager is concerned that we are not being compliant. Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Tue Aug 14 11:34:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 14 11:34:25 2007 Subject: [Histonet] Frozen section turnaround time In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD383@LBEXCH01.hchd.local> Message-ID: <903569.85123.qm@web61223.mail.yahoo.com> The TAT has to reffer to the FIRST section used for diagnosis; the following sections are additional sections, either levels or margins, and depend on the needs of the pathologist. You can argue that one thing is doing the first section and another is arriving to the diagnosis when more than 1 sections are needed. That diagnosis time should the TAT for the diagnosis (for the pathologist), not for the first section (your TAT). Probably your manager does not even know what you are talking about. Ren? J. "Scott, Allison D" wrote: Hello to everyone in histoland. I have a question concerning frozen section turnaround time. When you do your turnaround time report do you do it based on multiple sections or single sections. The bulk of our cases are multiple block frozens. They never meet the 20 min. time limit. The report that I turn in never meets the 100% compliance rate based on my numbers. Would someone be willing to share a report with me. My manager is concerned that we are not being compliant. Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. From Jessica.Vacca <@t> HCAhealthcare.com Tue Aug 14 11:46:08 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Tue Aug 14 11:47:20 2007 Subject: [Histonet] Frozen section turnaround time In-Reply-To: <903569.85123.qm@web61223.mail.yahoo.com> References: <1872B4A455B7974391609AD8034C79FC8BD383@LBEXCH01.hchd.local> <903569.85123.qm@web61223.mail.yahoo.com> Message-ID: <41E16A15CE78374EA45B57E0F94339B802AEEFAB@ORLEV01.hca.corpad.net> We clock the specimen in when it comes from surgery to the time we take it to the pathologist. We only measure the times of the first section taken, if it is a cone with 10 blocks the first block is the only one timed. Should we be having the Dr's write their time that the Dx was made as well? Jessica Vacca Histology Supervisor Brandon Regional Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 14, 2007 12:34 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen section turnaround time The TAT has to reffer to the FIRST section used for diagnosis; the following sections are additional sections, either levels or margins, and depend on the needs of the pathologist. You can argue that one thing is doing the first section and another is arriving to the diagnosis when more than 1 sections are needed. That diagnosis time should the TAT for the diagnosis (for the pathologist), not for the first section (your TAT). Probably your manager does not even know what you are talking about. Ren? J. "Scott, Allison D" wrote: Hello to everyone in histoland. I have a question concerning frozen section turnaround time. When you do your turnaround time report do you do it based on multiple sections or single sections. The bulk of our cases are multiple block frozens. They never meet the 20 min. time limit. The report that I turn in never meets the 100% compliance rate based on my numbers. Would someone be willing to share a report with me. My manager is concerned that we are not being compliant. Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> mshri.on.ca Tue Aug 14 12:14:23 2007 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Tue Aug 14 12:14:42 2007 Subject: [Histonet] Ovary aromatase Message-ID: Hi Valeria. I do a lot of mouse IHC (FFPE and frozen sections), and mouse tissue, *especially* ovary, is highly autofluorescent, so you have my sympathies. In fact, when I look at a fresh mouse ovary under the fluorescent dissecting microscope, it really lights up! One of the things I have found rather helpful in reducing the amount of autofluorescence is Sudan Black. So, you're supposed to do the autofluorescence quenching step after finishing the immunofluorescence stain and I have tried this, and it *does* work; however, I have had a couple of antibodies (anti-cytokeratin, for example) that don't like this treatment, and so I do the quenching after the blocking step. I would try both ways to see which one works best. I have tried different quenching times on different mouse tissues (not ovary, unfortunately) and have found that anywhere from 10-30 minutes is required. After quenching, wash the slide well with squirts of wash buffer to remove precipitates...I usually use a regular, plastic transfer pipette for this. Follow the squirting wash with a 10-minute wash in wash buffer. Then, either coverslip if you've finished your staining, or continue with your primary antibody incubation. Here is the protocol for preparing the Sudan Black solution: Make up 0.l% solution in 70% ethanol. Heat to boiling, then cool and filter. I think that's about it. Best of luck, Jacqui Detmar Samuel Lunenfeld Research Institute, Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 From karling <@t> usc.edu Tue Aug 14 12:44:09 2007 From: karling <@t> usc.edu (Kar Ling) Date: Tue Aug 14 12:44:27 2007 Subject: [Histonet] beta-tubulin staining of neurites In-Reply-To: <4B5700BE37215344A81185F131A848C261EB83@EVS6.its.caregroup.org> References: <4B5700BE37215344A81185F131A848C261EB83@EVS6.its.caregroup.org> Message-ID: I haven't do beta-tubulin staining, but I've done neurofilament staining. I think general procedures apply. For immunofluorescent staining for neuronal culture, the biggest problem is detachment of neurites. What I do is to fix them on ice and try not to remove all medium when you change medium. Start with neuron culture with culture medium 1. (optional step) withdraw half of the medium and replace with warm phosphate buffer saline pH 7.4 (PBS) . Repeat 2 times. 2. withdraw half of PBS and add equal volume of ice-cold 8% paraformaldehyde fixation, 10 min, on ice. 3. Washing with PBS, 5 min, 3 times 4. Blocking with 2% BSA (bovine serum albumin) 0.2% triton-X-100 in PBS, 30 min 5. incubate primary antibody in blocking buffer at 4C, overnite. 6. Washing with PBS, 5 min, 3 times 7. incubating secondary. (we usually use 1:1000) 8. Washing with PBS, 5 min, 3 times 9. mount and observe. Karen Ling University of Southern California Program in Neuroscience 3641 Watt Way, HNB 209 Los Angeles, CA 90089-2520 ----- Original Message ----- From: cbass@bidmc.harvard.edu Date: Tuesday, August 14, 2007 7:22 am Subject: [Histonet] beta-tubulin staining of neurites To: histonet@lists.utsouthwestern.edu > Hello Everyone, > > I'm looking for a decent protocol for staining beta-tubulin in > tissue culture. Basically, we are doing a neurite extension assay > in chamber wells, where the neurites extend onto the filter in > culture and then we stain the neurites for beta-tubulin. We use > the following reagents: > > SHS-Y5Y cells > beta-tubulin antibody, rabbit polyclonal, Cell Signaling, Cat #2146 > Alexafluor 488, goat anti-rabbit, invitrogen Cat # A11008 > > We are new to immunofluorescence so any help would be appreciated. > We are trying this out initially in cells plated in regular 24-well > plates. The best we've seen is a light diffuse staining with 1:500 > of the primary and 1:2000 of the secondary. My guess is that both > dilutions have to be cut down. Any suggestions? Also, should > there be a permeabilization step? I think we tried tween-20 in one > step. Do we need triton-x? > > Thanks everyone! > > Caroline > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Tue Aug 14 12:50:57 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Aug 14 12:51:12 2007 Subject: [Histonet] Metal staining dishes for BSL3 facility Message-ID: Hello All =20 I'm posting this for a friend. They are looking for a staining aparatus = that consists of metal staining dishes and racks for use in a BSL3 = laboratory. I have seen metal staining racks and lids to the glass = staining dishes but I have never seen metal staining dishes. Is anyone = out there familar with a vendor that sells metal staining dishes or = staining dishes that can be autoclaved. I suspect thats why they want = the metal. On the other hand if there is anyone out there that works in = a BSL3 lab and has any suggestions as to what they use that would be = great also. =20 =20 Thanks in advance =20 Liz =20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com HYPERLINK "http://www.premierlab.com/"www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.11.17/951 - Release Date: = 8/13/2007 10:15 AM =20 From gu.lang <@t> gmx.at Tue Aug 14 13:03:18 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 14 13:03:34 2007 Subject: [Histonet] microtome Message-ID: <004001c7de9d$65f0a550$6412a8c0@dielangs.at> Hi Maria, this type of microtome is not designed for making ribbons. I don't know any fast way to make a ribbon, but you can try to turn on the "retraction". This would lower the block, when you drive the knife back. First you have to catch the first slide, but let it stick on the knife edge. Then you make the next movement back and forward. The following slide will stick to the first. I think it is no practical way. If we need serial sections, we just take each slide coming after the other and mount them in the right order. Depending on the size we put 4-5 sections on the glass slide. - this would work faster, than playing around with a ribbon. Hope this helps Gudrun Lang From gu.lang <@t> gmx.at Tue Aug 14 13:15:34 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 14 13:15:50 2007 Subject: AW: [Histonet] Frozen section turnaround time In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD383@LBEXCH01.hchd.local> Message-ID: <004c01c7de9f$1c3d6360$6412a8c0@dielangs.at> What is the definition of TAT? I don't know anything about the requirements of "your" CAP, but I think it's a matter of definition. For my understanding turnaround time is the duration from receiving the specimen until the report to the surgeon. The report is the product of the histo-process. And it should matter, if you have to make one or ten frozen sections to get this goal. My thoughts. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Scott, Allison D Gesendet: Dienstag, 14. August 2007 18:09 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Frozen section turnaround time Hello to everyone in histoland. I have a question concerning frozen section turnaround time. When you do your turnaround time report do you do it based on multiple sections or single sections. The bulk of our cases are multiple block frozens. They never meet the 20 min. time limit. The report that I turn in never meets the 100% compliance rate based on my numbers. Would someone be willing to share a report with me. My manager is concerned that we are not being compliant. Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Tue Aug 14 13:37:50 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue Aug 14 13:38:24 2007 Subject: [Histonet] Re: beta tubulin Message-ID: <002001c7dea2$39e5d3e0$4101a8c0@carlba65530bda> Hi. you don't mention what fixing solution/ duration you use...... I have not used the primary Ab you mention: however, as it is not a neurone-specific Ab, you need to use another neurone-specific Ab to confirm that what you are demonstrating are neurones ( sure, morphology helps but is not neccessarily definitive) OR switch to a neurone-specific beta tubulin ( beta III tubulin- neurone specific). Otherwise , it seems to me that your technique is about right, provided that you are permeabilising after fixation but before primary Ab incubation. You will need to permeabilise to optimise staining, dependant on all the other variables in your technique, that you haven't mentioned NB: Can't comment on appropriate dilution of your primary Ab but your Alexafluor 488 should be fine......altho' I use it at 1/1000 dilution factor. Have a look at IF pics of neurone -specific beta tubulin here, for example : http://www.immunoportal.com/ Best of luck Carl From gcallis <@t> montana.edu Tue Aug 14 13:52:42 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Aug 14 13:52:44 2007 Subject: [Histonet] Metal staining dishes for BSL3 facility In-Reply-To: References: Message-ID: <6.0.0.22.1.20070814124048.01b12158@gemini.msu.montana.edu> Dear Liz, Shandon Complete Staining Assembly 117 is stainless steel dishes for 60 slides and are very pricey. I did a search with Thermo Shandon as a key word. I have not seen the 30 slide capacity for a long time, but Shandon-Lipshaw (many, many names/mergers ago) had them. We also have stainless steel pans, with high sides that look like bread pans (both small and large) and a slide rack for 20 slides fits in the smaller ones (sideways) and two of the 30 slide capacity or 60 slide capacity fits in the larger one. You may find these as they were originally used to autoclave instruments, or whatever - as for a lid, one could devise something or cover with foil. For our BSL3, staining dishes will never be taken out of the facility. Consequently, the dishes purchased are chemically resistant dishes from Ted Pella. Be sure to check if they really need to have autoclavable as long as the dishes are never brought out of BSL3 containment. If they keep this type of item inside the BSL3, then you should be able to buy regular staining dishes. Good luck Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 11:50 AM 8/14/2007, you wrote: >Hello All > >I'm posting this for a friend. They are looking for a staining aparatus >that consists of metal staining dishes and racks for use in a BSL3 >laboratory. I have seen metal staining racks and lids to the glass >staining dishes but I have never seen metal staining dishes. Is anyone >out there familar with a vendor that sells metal staining dishes or >staining dishes that can be autoclaved. I suspect thats why they want the >metal. On the other hand if there is anyone out there that works in a >BSL3 lab and has any suggestions as to what they use that would be great >also. > >Thanks in advance > >Liz > From ploykasek <@t> phenopath.com Tue Aug 14 14:15:25 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Aug 14 14:15:39 2007 Subject: [Histonet] RE: Histonet Digest, Vol 45, Issue 18 In-Reply-To: <414FA20EC03C3140924A19ACF103D2974C1315@RHAEX2.RHA-RRS.CA> Message-ID: Hi Mary Lee. Is the antibody perhaps Galectin-3? Galectin-3 is useful in distinguishing thyroid neoplasms. We are in the process of working up a Galectin-3 antibody clone 9C4 from Vector Laboratories. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Help...again, > Does anybody have any info. on calectin-3 ? What is it? Who uses it? Where do > you get it? > Does anybody else use the Breast Receptor Block from Zymed ? If so how do you > find it works for you? > Thanks for listening. > Mary Lee Danahy R.T.,M.L.T.III > Immunohistochemistry/Molecular Lab. > Pathology Dept. > Atlantic Health Sciences Corporation > Saint John N.B.Canada > tel.# 506-648-6604 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 2:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 45, Issue 18 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: > Contents of Histonet digest..." > > > Today's Topics: > > 1. Starfrost slides IHC (Melinda Goodyear) > 2. (no subject) (MARY T HODGES) > 3. Re: mouse spinal cord atlas (Geoff McAuliffe) > 4. Re: mouse spinal cord atlas (Emily Sours) > 5. RELIA Special Job Alert for Managers, Supervisors and Lead > Techs (Pam Barker) > 6. Xylene substitutes (Kalleberg, Kristopher) > 7. Re: mouse spinal cord atlas (Geoff McAuliffe) > 8. Re: Xylene substitutes (Rene J Buesa) > 9. Pre-filled containers of B-5 fixative (MICHELLE MCNEESE) > 10. RE: Pre-filled containers of B-5 fixative (Weems, Joyce) > 11. RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative > (Douglas D Deltour) > 12. Looking for book (Perry, Margaret) > 13. Re: Looking for book (Jackie M O'Connor) > 14. Re: Looking for book (Jackie M O'Connor) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 13 Aug 2007 17:27:50 +1000 > From: "Melinda Goodyear" > Subject: [Histonet] Starfrost slides IHC > To: > Message-ID: > <61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8@EXCHANGE.ltu.edu.au> > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > > > I have been in contact with a third party from Australia that distributes > Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH (Knittel > Glaser) as my tissue sections have been falling off during > immunohistochemistry using a new batch of slides (Starfrost Adhesive > hydrophilic slides) with the same catalogue number as previously ordered. I > received an explanation that all previous batches of slides sent may have been > mis-labelled and probably had an adhesive applied like silane coated or > poly-L-lysine. The only difference between the old batch of slides and new > batch is that the old ones have 2 stars printed at the top of the slides and > the boxes are labelled as "adhesive-Objekttrager" rather than "Objekttrager". > I am wondering whether anybody else may be have been having problems with > these slides and whether changing to silane coated may help? > > > > Thanks in advance for your help, > > > > Melinda > > Visual Neuroscience Lab > > School of Psychological Science > > Faculty of Science, Technology and Engineering > > La Trobe University > > Victoria, 3086 > > PH: +61 3 9479 2470 > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 13 Aug 2007 07:40:01 -0400 > From: "MARY T HODGES" > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > Has anyone had any trouble confirming their regristry from ASCP? > I have completely dropped out of the system and don't know what or who > to contact. > Tere Hodges > Tucson, Az > _________________________________________________________________ > > [1]Booking a flight? Know when to buy with airfare predictions on MSN > Travel. > > References > > 1. http://g.msn.com/8HMAENUS/2755??PS=47575 > > > ------------------------------ > > Message: 3 > Date: Mon, 13 Aug 2007 09:28:22 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] mouse spinal cord atlas > To: Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46C05C76.9020408@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > Greetings all: > > The link Emily supplied does not work and I could not find "The House Mouse" > at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green (former > director of the Jackson Lab) might have the information the original poster > was seeking. > > Geoff > > Emily Sours wrote: >> The best atlas I have heard of dealing with the mouse spinal cord is >> called "The House Mouse." It is no longer being published but the >> amazon link is here tinyuRL.com/2rnvul You may want to see if it's >> been updated with a more recent name. If not, try to find a used copy >> with bookfinder.com I haven't personally used this, but my boss swears >> by it, and she's an old school anatomist of mice and chicks. >> >> Emily >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > > ------------------------------ > > Message: 4 > Date: Mon, 13 Aug 2007 09:42:13 -0400 > From: "Emily Sours" > Subject: Re: [Histonet] mouse spinal cord atlas > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Here's a long link for The House Mouse at amazon > http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059407/re > f=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2 > here's another try for the short one: > http://www.tinyurl.com/2rnvul > > > Emily > -- > these things happen, you know, you go for a walk in the park one day and > wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and > dinosaurs show up to eat the remains. > > > > ------------------------------ > > Message: 5 > Date: Mon, 13 Aug 2007 10:41:13 -0400 > From: "Pam Barker" > Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors > and Lead Techs > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters, > I have several exciting opportunities for experienced Histology > Managers, Supervisors and Lead Technicians in hospital and private lab > environments in several locations nationwide. The compensation packages > are excellent, the work is challenging and the sky is the limit. The > positions are of course full time and day shift. > > > > Here are my leadership positions: > > Histology Manager - Central CA, Near Santa Barbara > > Histology Supervisor - Gulf Coast, Texas > > Histology Supervisor - Southeastern MA > > Histology Manager - Chicago, IL > > > If you would like more information or know of someone else who might be > interested, please contact me at relia1@earthlink.net or 866-607-3542. > I am available to discuss the opportunity at your convenience including > after hours. Thanks-Pam > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > > > ------------------------------ > > Message: 6 > Date: Mon, 13 Aug 2007 11:15:53 -0400 > From: "Kalleberg, Kristopher" > Subject: [Histonet] Xylene substitutes > To: > Message-ID: > <0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76@NTRSEVS30002.s3.ms.unilever.com> > > Content-Type: text/plain; charset="us-ascii" > > Is anyone out there familiar with Xylene substitutes such as > Histo-Clear? I am wondering if switching over the the substitutes is a > good idea. Any positive or negative feedback would be greatly > appreciated when it comes to IHC results. Thanks in adavance. > > Kris > > > ------------------------------ > > Message: 7 > Date: Mon, 13 Aug 2007 11:14:55 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] mouse spinal cord atlas > To: Kar Ling > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46C0756F.6000507@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > You might have a look at Progress in Brain Research 11:1-56, 1964 by > Nieuwenhuys, a long paper on comparative anatomy of the spinal cord. > > Geoff > > > Kar Ling wrote: >> Hi, >> >> I have problems in defining ventral roots of lumbar spinal segments in mice. >> I try to look up some mouse anatomy book, but it only has bunch of spinal >> cord cross section. For gross anatomy of the spinal cord, I can only find >> rat ones. I wonder if there's such book with whole spinal cord illustration. >> >> My first questions is: Do mice has L1-L6 and S1-S4 as in rat? >> >> My way to dissect spinal cord is cutting the dorsal bones piece by pieces, >> and then carefully remove the ventral bones starting at the sides so that I >> can preserve DRG attached to both ventral and dorsal roots. (that took me an >> hour to expose sacral and lumber segments). >> >> If I count from conus medullaris, how can I be sure my counting is correct? >> should I count main nerves or emerging branches? Is there any hallmarks that >> can differentiate L6 from S1? >> >> According to what I see in mouse spinal cord, the last thick ventral spinal >> nerve coming out from the spinal cord appears to be several branches emerging >> from the cord then merging into one, but then split into 2 again. One of >> those nerves attach another dorsal root and DRG, yet I fail to preserve the >> other DRG if any. As for the 2 ventral nerves below this one are much >> thinner and also has few emerging branches from the cord. So, I am not sure >> about what I see is L6 or it's a merge of L6 & S1?? >> >> If I count the afforementioned nerve as L6, I can locate 3 pairs of long >> dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above. >> Yet, L1 roots are notably longer than Thoracic root along rib cage level. So, >> Is L1 always longer than T12?? >> >> I read some histonet achieve suggesting saline injection to push the spinal >> cord out, can the DRG be preserved? >> >> Thank you. >> >> Karen Ling >> University of Southern California >> Program in Neuroscience >> 3641 Watt Way, HNB 209 >> Los Angeles, CA 90089-2520 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > > ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From RSRICHMOND <@t> aol.com Tue Aug 14 14:31:47 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Aug 14 14:32:08 2007 Subject: [Histonet] Re: Frozen section turnaround time Message-ID: Allison Scott (where's LBJ Hospital - is that the LBJ I think it is?) asks about turnaround time for frozen sections. That's the time from when you receive the specimen in the laboratory until the time when the pathologist phones the report to the surgeons. If I understand it aright, CAP's requirement is 20 minutes for a single frozen section, but there are no CAP guidelines for multiple frozen sections. The problem is getting the pathologist to record the time on the frozen section work sheet - it's a real nuisance, and a lot of fudging of records can be expected, but the Herrn Inschpektors have no way of knowing that. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From RSRICHMOND <@t> aol.com Tue Aug 14 14:37:04 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Aug 14 14:37:19 2007 Subject: [Histonet] Re: Alcian yellow Message-ID: As many of you know, Dick Dapson at Anatech worked out an environmentally safe synthesis for Alcian blue several years ago, and this manufacturing process is actually in use. Dapson reported that he could synthesize Alcian yellow, but that the product did not have a satisfactory shelf life, and he eventually abandoned it and substituted a different yellow dye. Both Alcian blue and Alcian yellow are synthesized by the old environmentally unsafe methods in countries that do not concern themselves about the safety of workers. If such countries are the only source of Alcian yellow, I would be reluctant to buy it, particularly since a satisfactory substitute is available. I have no connection with Anatech, and I have no personal experience with any of the yellow dyes involved here. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From vazquezr <@t> ohsu.edu Tue Aug 14 13:37:36 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Aug 14 14:59:51 2007 Subject: [Histonet] Frozen section turnaround time Message-ID: Allison, The TAT, it all depends on several instants. For instance, how many did they bring at once or one at a time? One at a time, you could have it embedded, cut and on the stainer in 5 minutes. The stainer is the long portion of it (10 min or so). 4-5 pieces of tissue, yes 20 minutes minimum. But then, the ability to get the doctor to come and read it is a whole other issue. I would say base it on 2 pieces of tissue, which is the norm for the first stage. Just my two cents Robyn 503-494-2314 >>> "Rene J Buesa" 8/14/2007 9:34 AM >>> The TAT has to reffer to the FIRST section used for diagnosis; the following sections are additional sections, either levels or margins, and depend on the needs of the pathologist. You can argue that one thing is doing the first section and another is arriving to the diagnosis when more than 1 sections are needed. That diagnosis time should the TAT for the diagnosis (for the pathologist), not for the first section (your TAT). Probably your manager does not even know what you are talking about. Ren? J. "Scott, Allison D" wrote: Hello to everyone in histoland. I have a question concerning frozen section turnaround time. When you do your turnaround time report do you do it based on multiple sections or single sections. The bulk of our cases are multiple block frozens. They never meet the 20 min. time limit. The report that I turn in never meets the 100% compliance rate based on my numbers. Would someone be willing to share a report with me. My manager is concerned that we are not being compliant. Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Aug 14 14:59:11 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Aug 14 15:00:14 2007 Subject: [Histonet] RE: Histonet Digest, Vol 45, Issue 18 In-Reply-To: Message-ID: <000101c7dead$9807b960$d00f7ca5@lurie.northwestern.edu> Labvision (now a part of Fisher)also sells it (galectin-3). Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, August 14, 2007 2:15 PM To: Danahy, Mary-Lee (R2); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Histonet Digest, Vol 45, Issue 18 Hi Mary Lee. Is the antibody perhaps Galectin-3? Galectin-3 is useful in distinguishing thyroid neoplasms. We are in the process of working up a Galectin-3 antibody clone 9C4 from Vector Laboratories. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Help...again, > Does anybody have any info. on calectin-3 ? What is it? Who uses it? Where do > you get it? > Does anybody else use the Breast Receptor Block from Zymed ? If so how do you > find it works for you? > Thanks for listening. > Mary Lee Danahy R.T.,M.L.T.III > Immunohistochemistry/Molecular Lab. > Pathology Dept. > Atlantic Health Sciences Corporation > Saint John N.B.Canada > tel.# 506-648-6604 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 2:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 45, Issue 18 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: > Contents of Histonet digest..." > > > Today's Topics: > > 1. Starfrost slides IHC (Melinda Goodyear) > 2. (no subject) (MARY T HODGES) > 3. Re: mouse spinal cord atlas (Geoff McAuliffe) > 4. Re: mouse spinal cord atlas (Emily Sours) > 5. RELIA Special Job Alert for Managers, Supervisors and Lead > Techs (Pam Barker) > 6. Xylene substitutes (Kalleberg, Kristopher) > 7. Re: mouse spinal cord atlas (Geoff McAuliffe) > 8. Re: Xylene substitutes (Rene J Buesa) > 9. Pre-filled containers of B-5 fixative (MICHELLE MCNEESE) > 10. RE: Pre-filled containers of B-5 fixative (Weems, Joyce) > 11. RE: {SPAM?} [Histonet] Pre-filled containers of B-5 fixative > (Douglas D Deltour) > 12. Looking for book (Perry, Margaret) > 13. Re: Looking for book (Jackie M O'Connor) > 14. Re: Looking for book (Jackie M O'Connor) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 13 Aug 2007 17:27:50 +1000 > From: "Melinda Goodyear" > Subject: [Histonet] Starfrost slides IHC > To: > Message-ID: > <61B1A7B85B73F44B9DBCB9F16C8D1C8202A30AE8@EXCHANGE.ltu.edu.au> > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > > > I have been in contact with a third party from Australia that distributes > Starfrost slides made by Waldemar Knittel Glasbearbeitungs GmbH (Knittel > Glaser) as my tissue sections have been falling off during > immunohistochemistry using a new batch of slides (Starfrost Adhesive > hydrophilic slides) with the same catalogue number as previously ordered. I > received an explanation that all previous batches of slides sent may have been > mis-labelled and probably had an adhesive applied like silane coated or > poly-L-lysine. The only difference between the old batch of slides and new > batch is that the old ones have 2 stars printed at the top of the slides and > the boxes are labelled as "adhesive-Objekttrager" rather than "Objekttrager". > I am wondering whether anybody else may be have been having problems with > these slides and whether changing to silane coated may help? > > > > Thanks in advance for your help, > > > > Melinda > > Visual Neuroscience Lab > > School of Psychological Science > > Faculty of Science, Technology and Engineering > > La Trobe University > > Victoria, 3086 > > PH: +61 3 9479 2470 > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 13 Aug 2007 07:40:01 -0400 > From: "MARY T HODGES" > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > Has anyone had any trouble confirming their regristry from ASCP? > I have completely dropped out of the system and don't know what or who > to contact. > Tere Hodges > Tucson, Az > _________________________________________________________________ > > [1]Booking a flight? Know when to buy with airfare predictions on MSN > Travel. > > References > > 1. http://g.msn.com/8HMAENUS/2755??PS=47575 > > > ------------------------------ > > Message: 3 > Date: Mon, 13 Aug 2007 09:28:22 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] mouse spinal cord atlas > To: Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46C05C76.9020408@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > Greetings all: > > The link Emily supplied does not work and I could not find "The House Mouse" > at amazon.com. "The Biology of the Laboratory Mouse", by Earl Green (former > director of the Jackson Lab) might have the information the original poster > was seeking. > > Geoff > > Emily Sours wrote: >> The best atlas I have heard of dealing with the mouse spinal cord is >> called "The House Mouse." It is no longer being published but the >> amazon link is here tinyuRL.com/2rnvul You may want to see if it's >> been updated with a more recent name. If not, try to find a used copy >> with bookfinder.com I haven't personally used this, but my boss swears >> by it, and she's an old school anatomist of mice and chicks. >> >> Emily >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > > ------------------------------ > > Message: 4 > Date: Mon, 13 Aug 2007 09:42:13 -0400 > From: "Emily Sours" > Subject: Re: [Histonet] mouse spinal cord atlas > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Here's a long link for The House Mouse at amazon > http://www.amazon.com/House-Mouse-Atlas-Embryonic-Development/dp/3540059 407/re > f=sr_1_2/105-5355714-4615608?ie=UTF8&s=books&qid=1186808006&sr=8-2 > here's another try for the short one: > http://www.tinyurl.com/2rnvul > > > Emily > -- > these things happen, you know, you go for a walk in the park one day and > wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and > dinosaurs show up to eat the remains. > > > > ------------------------------ > > Message: 5 > Date: Mon, 13 Aug 2007 10:41:13 -0400 > From: "Pam Barker" > Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors > and Lead Techs > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters, > I have several exciting opportunities for experienced Histology > Managers, Supervisors and Lead Technicians in hospital and private lab > environments in several locations nationwide. The compensation packages > are excellent, the work is challenging and the sky is the limit. The > positions are of course full time and day shift. > > > > Here are my leadership positions: > > Histology Manager - Central CA, Near Santa Barbara > > Histology Supervisor - Gulf Coast, Texas > > Histology Supervisor - Southeastern MA > > Histology Manager - Chicago, IL > > > If you would like more information or know of someone else who might be > interested, please contact me at relia1@earthlink.net or 866-607-3542. > I am available to discuss the opportunity at your convenience including > after hours. Thanks-Pam > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > > > ------------------------------ > > Message: 6 > Date: Mon, 13 Aug 2007 11:15:53 -0400 > From: "Kalleberg, Kristopher" > Subject: [Histonet] Xylene substitutes > To: > Message-ID: > <0E6BC087F70F9C47ACFF2C203D6E329C02CFEC76@NTRSEVS30002.s3.ms.unilever.co m> > > Content-Type: text/plain; charset="us-ascii" > > Is anyone out there familiar with Xylene substitutes such as > Histo-Clear? I am wondering if switching over the the substitutes is a > good idea. Any positive or negative feedback would be greatly > appreciated when it comes to IHC results. Thanks in adavance. > > Kris > > > ------------------------------ > > Message: 7 > Date: Mon, 13 Aug 2007 11:14:55 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] mouse spinal cord atlas > To: Kar Ling > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46C0756F.6000507@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > You might have a look at Progress in Brain Research 11:1-56, 1964 by > Nieuwenhuys, a long paper on comparative anatomy of the spinal cord. > > Geoff > > > Kar Ling wrote: >> Hi, >> >> I have problems in defining ventral roots of lumbar spinal segments in mice. >> I try to look up some mouse anatomy book, but it only has bunch of spinal >> cord cross section. For gross anatomy of the spinal cord, I can only find >> rat ones. I wonder if there's such book with whole spinal cord illustration. >> >> My first questions is: Do mice has L1-L6 and S1-S4 as in rat? >> >> My way to dissect spinal cord is cutting the dorsal bones piece by pieces, >> and then carefully remove the ventral bones starting at the sides so that I >> can preserve DRG attached to both ventral and dorsal roots. (that took me an >> hour to expose sacral and lumber segments). >> >> If I count from conus medullaris, how can I be sure my counting is correct? >> should I count main nerves or emerging branches? Is there any hallmarks that >> can differentiate L6 from S1? >> >> According to what I see in mouse spinal cord, the last thick ventral spinal >> nerve coming out from the spinal cord appears to be several branches emerging >> from the cord then merging into one, but then split into 2 again. One of >> those nerves attach another dorsal root and DRG, yet I fail to preserve the >> other DRG if any. As for the 2 ventral nerves below this one are much >> thinner and also has few emerging branches from the cord. So, I am not sure >> about what I see is L6 or it's a merge of L6 & S1?? >> >> If I count the afforementioned nerve as L6, I can locate 3 pairs of long >> dorsal and ventral roots (L6-L3) and 2 pairs of relatively short L2-L1 above. >> Yet, L1 roots are notably longer than Thoracic root along rib cage level. So, >> Is L1 always longer than T12?? >> >> I read some histonet achieve suggesting saline injection to push the spinal >> cord out, can the DRG be preserved? >> >> Thank you. >> >> Karen Ling >> University of Southern California >> Program in Neuroscience >> 3641 Watt Way, HNB 209 >> Los Angeles, CA 90089-2520 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > > ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dahmed <@t> mdanderson.org Tue Aug 14 15:12:16 2007 From: dahmed <@t> mdanderson.org (dahmed@mdanderson.org) Date: Tue Aug 14 15:12:47 2007 Subject: [Histonet] Histology Technician Position Available in Houston, Texas Message-ID: Hello Histonetters, M.D. Anderson in Houston, Texas has a full-time Histology technician position available. Interested applicants may apply on the website- www.mdanderson.org under Careers with a keyword search for Histology. Thank you. David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center From Wanda.Smith <@t> HCAhealthcare.com Tue Aug 14 16:12:20 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Aug 14 16:12:39 2007 Subject: [Histonet] Re: Frozen section turnaround time In-Reply-To: References: Message-ID: <817C2761C5A1394180709AEEDB775B7E032966A7@NASEV03.hca.corpad.net> Bob, AMEN to getting the Pathologist to sign the worksheet!!!! I have threatened to break their legs is they walk off without signing. Regarding the 20 minute TAT, Mr. Gruber at CAP told me that the 20 minutes is from the time the Pathologist starts the frozen section to the time they call the surgeon. That makes sense if the Pathologist are responsible for frozen sections at a remote site that they have to travel to. In the narrative after the question in the checklist, it states: "NOTE: If 90% of frozen sections are not completed within 20 minutes, the laboratory must document evaluation of the reason(s) for the delay. This TAT is intended to apply to the typical single frozen section. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer TAT may be expected" Hope this helps!!! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Tuesday, August 14, 2007 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Frozen section turnaround time Allison Scott (where's LBJ Hospital - is that the LBJ I think it is?) asks about turnaround time for frozen sections. That's the time from when you receive the specimen in the laboratory until the time when the pathologist phones the report to the surgeons. If I understand it aright, CAP's requirement is 20 minutes for a single frozen section, but there are no CAP guidelines for multiple frozen sections. The problem is getting the pathologist to record the time on the frozen section work sheet - it's a real nuisance, and a lot of fudging of records can be expected, but the Herrn Inschpektors have no way of knowing that. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bills <@t> icpmr.wsahs.nsw.gov.au Tue Aug 14 16:30:37 2007 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Tue Aug 14 16:31:05 2007 Subject: [Histonet] Re: Frozen section turnaround time In-Reply-To: <817C2761C5A1394180709AEEDB775B7E032966A7@wsahs.nsw.gov.au> Message-ID: <000e01c7deba$5b446200$436b080a@wsahs.nsw.gov.au> Dear All, Hello from Sydney, Australia, In my laboratory most of the pathologists sign the F/S worksheet with both the start and finish times for each Frozen Section block. They tend to use it as a measure of how efficient they are when the TAT audit figures appear at their Medical Staff meetings. Admittedly most of the time the specimen is selected by a pathology registrar and the pathologist only arrives to read the report with the registrar towards the end. Our TAT for each single block has averaged 15mins from time of arrival to report issue for the last 15 years. This often includes the F/S pathologist referring the case to a pathologist within whose specialty the specimen falls. We have in the F/S area a Cryostat, Biohazard Cabinet, staining solutions and a double head microscope for the Pathologist and Registrar to use to diagnose and a telephone so that the report can be issued directly to the theatre. Bill Sinai Chief Hospital Scientist Tissue Pathology ICPMR Westmead Hospital Westmead NSW 2145 Phone 02 9845 7774 Fax 02 9687 2330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, 15 August 2007 7:12 AM To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Frozen section turnaround time Bob, AMEN to getting the Pathologist to sign the worksheet!!!! I have threatened to break their legs is they walk off without signing. Regarding the 20 minute TAT, Mr. Gruber at CAP told me that the 20 minutes is from the time the Pathologist starts the frozen section to the time they call the surgeon. That makes sense if the Pathologist are responsible for frozen sections at a remote site that they have to travel to. In the narrative after the question in the checklist, it states: "NOTE: If 90% of frozen sections are not completed within 20 minutes, the laboratory must document evaluation of the reason(s) for the delay. This TAT is intended to apply to the typical single frozen section. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer TAT may be expected" Hope this helps!!! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Tuesday, August 14, 2007 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Frozen section turnaround time Allison Scott (where's LBJ Hospital - is that the LBJ I think it is?) asks about turnaround time for frozen sections. That's the time from when you receive the specimen in the laboratory until the time when the pathologist phones the report to the surgeons. If I understand it aright, CAP's requirement is 20 minutes for a single frozen section, but there are no CAP guidelines for multiple frozen sections. The problem is getting the pathologist to record the time on the frozen section work sheet - it's a real nuisance, and a lot of fudging of records can be expected, but the Herrn Inschpektors have no way of knowing that. Bob Richmond Samurai Pathologist Knoxville TN ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Sydney West Area Health Service (SWAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify SWAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of SWAHS. From TreseMoles <@t> neo.rr.com Tue Aug 14 16:48:42 2007 From: TreseMoles <@t> neo.rr.com (TreseMoles@neo.rr.com) Date: Tue Aug 14 16:49:20 2007 Subject: [Histonet] Re: Frozen section turnaround time In-Reply-To: References: Message-ID: <8ffe8908a0d6.a0d68ffe8908@columbus.rr.com> That's how we do it here at Akron Children's as well. We have a Frozen Section worksheet right next to the microscope that they use for frozen's... the pathologist also puts down who they spoke with in the OR, to convey the results. Our FS volume isn't that huge-- therefore- easy to implement. ----- Original Message ----- From: RSRICHMOND@aol.com Date: Tuesday, August 14, 2007 3:34 pm Subject: [Histonet] Re: Frozen section turnaround time To: histonet@lists.utsouthwestern.edu > Allison Scott (where's LBJ Hospital - is that the LBJ I think it > is?) asks > about turnaround time for frozen sections. That's the time from > when you receive > the specimen in the laboratory until the time when the pathologist > phones the > report to the surgeons. > > If I understand it aright, CAP's requirement is 20 minutes for a > single > frozen section, but there are no CAP guidelines for multiple > frozen sections. The > problem is getting the pathologist to record the time on the > frozen section > work sheet - it's a real nuisance, and a lot of fudging of records > can be > expected, but the Herrn Inschpektors have no way of knowing that. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > ************************************** > Get a sneak peek of the > all-new AOL at http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From adesupo2002 <@t> hotmail.com Tue Aug 14 18:52:02 2007 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Tue Aug 14 18:52:15 2007 Subject: [Histonet] HER-2 CONTROL TISSUE BLOCK Message-ID: Hi, Please I will appreciate it, if yo 87108.u guys can send a tissue block positive for HER-2 to me. We want to use it as control for running our HER-2 test. Hoping to hear from you guys asap. Adesupo Adesuyi 531 ORTIZ DR SE APART.D ALBUQUERQUE, NM 87108 _________________________________________________________________ Connect to the next generation of MSN Messenger? http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From cbass <@t> bidmc.harvard.edu Tue Aug 14 23:30:03 2007 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Tue Aug 14 23:30:16 2007 Subject: [Histonet] Re: beta tubulin In-Reply-To: <002001c7dea2$39e5d3e0$4101a8c0@carlba65530bda> References: <002001c7dea2$39e5d3e0$4101a8c0@carlba65530bda> Message-ID: <247FC828-BEC4-4BF2-B398-68BE44356A5C@bidmc.harvard.edu> Hello, Thanks for the advice. I am posting on behalf of someone in the lab and I don't have all of the details. However, from what I understand, she is doing a methanol fixation. I would prefer formalin, but she has been doing methanol for all her other staining procedures with these cells. Why is a neuron specific tubulin necessary, and is it for a cell line or primary cells? Caroline On Aug 14, 2007, at 2:37 PM, Carl Hobbs wrote: > Hi. > you don't mention what fixing solution/ duration you use...... > I have not used the primary Ab you mention: however, as it is not a > neurone-specific Ab, you need to use another neurone-specific Ab to > confirm that what you are demonstrating are neurones ( sure, > morphology helps but is not neccessarily definitive) OR switch to a > neurone-specific beta tubulin ( beta III tubulin- neurone specific). > Otherwise , it seems to me that your technique is about right, > provided that you are permeabilising after fixation but before > primary Ab incubation. You will need to permeabilise to optimise > staining, dependant on all the other variables in your technique, > that you haven't mentioned > NB: Can't comment on appropriate dilution of your primary Ab but > your Alexafluor 488 should be fine......altho' I use it at 1/1000 > dilution factor. > Have a look at IF pics of neurone -specific beta tubulin here, for > example : http://www.immunoportal.com/ > Best of luck > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cbass <@t> bidmc.harvard.edu Tue Aug 14 23:46:41 2007 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Tue Aug 14 23:46:52 2007 Subject: [Histonet] quick and easy sectioning of fresh brain Message-ID: <756D65C0-7DC5-4302-A4FC-BAF3AFA8CEB2@bidmc.harvard.edu> Hey Folks, So I want to have my cake and eat it too. I have injected a rat with a virus that will express EGFP. Ideally I'd like to collect the fresh brain, cut off thick floating sections, enough to get a feel for the spread of the virus, and possible punch out the EGFP area to get mRNA and/or protein for qPCR and western blot. Are there any suggestions for doing this? I have access to cryostats, sliding microtomes, and brain molds. Cutting fresh brain is difficult, so I'd like to freeze it to section on the sliding microtome, collect a floating section, and visualize under a fluorescent dissecting microscope. Are there any issues with doing this? How should I freeze the brain, and how will this affect mRNA and protein levels? How difficult are unfixed floating sections to deal with? Finally, can I take some sections and fix by immersion in PFA so that I can store for longer periods of time. Any suggestions would be appreciated. I've had very good luck working with perfused/fixed tissue, and would like to continue with it, but it doesn't seem possible with the mRNA/protein endpoints. Thanks, Caroline From ryan <@t> upei.ca Wed Aug 15 00:02:05 2007 From: ryan <@t> upei.ca (ryan@upei.ca) Date: Wed Aug 15 00:04:38 2007 Subject: [Histonet] Histonet Digest, Vol 45, Issue 21 Message-ID: <9562BC148F0@acad1.cs.upei.ca> C.L. Ryan will be out of the office from Friday Aug 10 until Monday Aug. 13, incl. From sonya.martin <@t> soton.ac.uk Wed Aug 15 05:59:37 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Aug 15 06:00:23 2007 Subject: [Histonet] Safety advice for non-toxic clearing agents Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40FD5D338@ISS-CL-EX-V1.soton.ac.uk> Hi All, I have recently tried Methyl (or should that be Ethyl!!) Green counterstain with DAB and find that it gives much better contrast than my haematoxylin. I only do frozen sections and I have everything set up so that I can use an aqueous mountant. For the Methyl Green (Vector) I need to dehydrate and clear before permanent mounting. The problem is I do not have easy access to a fume cabinet. I have Histoclear from RA Lamb and Vecta Permanent Mountant both of which are classed as non-toxic so I'm presuming I can use these on the bench. I only need to do this now and again so I wouldn't have the staining baths permanently on the bench. Just wanted to get a bit of feedback from you wise histonetters about wether or not this sounds 'safe'. Thanks Sonya From Barry.R.Rittman <@t> uth.tmc.edu Wed Aug 15 06:44:56 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Aug 15 06:45:34 2007 Subject: [Histonet] Safety advice for non-toxic clearing agents References: <71437982F5B13A4D9A5B2669BDB89EE40FD5D338@ISS-CL-EX-V1.soton.ac.uk> Message-ID: Sonya I personally treat ALL reagents as potentially harmful. The term "non toxic" is greatly misused. I feel that it should state "non toxic as far as has been determined". There are numerous instances in the literature regarding agents that originally were regarded as "safe" but later proved to be toxic especially after long term exposure, even if individual exposure times were short. A factor that is often not considered is that individuals may vary considerably in their responses to chemicals. Unfortunately while many agencies try their best to determine "acceptable" exposure levels, many of the studies are carried out on animals and the results from these studies cannot always be extrapolated to humans. Can you persuade your administrator to get you a vented chamber with appropriate filters that you can store most of the time and then bring out for the times you need it? Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Martin S. Sent: Wed 8/15/2007 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety advice for non-toxic clearing agents Hi All, I have recently tried Methyl (or should that be Ethyl!!) Green counterstain with DAB and find that it gives much better contrast than my haematoxylin. I only do frozen sections and I have everything set up so that I can use an aqueous mountant. For the Methyl Green (Vector) I need to dehydrate and clear before permanent mounting. The problem is I do not have easy access to a fume cabinet. I have Histoclear from RA Lamb and Vecta Permanent Mountant both of which are classed as non-toxic so I'm presuming I can use these on the bench. I only need to do this now and again so I wouldn't have the staining baths permanently on the bench. Just wanted to get a bit of feedback from you wise histonetters about wether or not this sounds 'safe'. Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMEarle <@t> CapeCodHealth.org Wed Aug 15 07:12:23 2007 From: PMEarle <@t> CapeCodHealth.org (Earle, Paul M.) Date: Wed Aug 15 07:12:30 2007 Subject: [Histonet] Requested VIP 3000 Manual Message-ID: <29688FCA0750AD4FB55B18D961EB91CC1016D3@cchex2.cchdomain1.capecodhealth.org> Nancy Rutledge and I thank you all so much for your responses! Patricia Adams from Alabama not only responded immediately, but sent an extra to us at her expense! This is really a "tight" group. Paul Paul M. Earle M.S., P.A. (ASCP) Anatomic Pathology Supervisor Pathology Department Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 508-548-5300 x73488 <> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From daydawning <@t> wideopenwest.com Wed Aug 15 08:34:30 2007 From: daydawning <@t> wideopenwest.com (daydawning@wideopenwest.com) Date: Wed Aug 15 08:34:35 2007 Subject: [Histonet] Re: Histonet Digest, Vol 45, Issue 21 In-Reply-To: <200708150447.l7F4lZAB030523@smtp-6.wideopenwest.com> References: <200708150447.l7F4lZAB030523@smtp-6.wideopenwest.com> Message-ID: <20070815132747.M14524@wideopenwest.com> Caroline, I think you require a vibrating blade microtome. It is designed to cut fresh tissue while submerged in cooled buffer. You attach the fresh brain to a disk with (believe it or not) crazy glue and the blade slowly slices through the tissue and it floats off into the buffer. It is more gentle on the tissue than freezing and sectioning in a cryostat. I'm sure you could find someone in your facillity that has one. Check out microm's website. the model is the 650V Dawn Truscott, HT(ASCP) Biocare Medical Personal Email > > Message: 19 > Date: Wed, 15 Aug 2007 00:46:41 -0400 > From: Caroline Bass > Subject: [Histonet] quick and easy sectioning of fresh brain > To: Histonet > Message-ID: <756D65C0-7DC5-4302-A4FC-BAF3AFA8CEB2@bidmc.harvard.edu> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hey Folks, > > So I want to have my cake and eat it too. I have injected a rat > with a virus that will express EGFP. Ideally I'd like to collect > the fresh brain, cut off thick floating sections, enough to get a > feel for the spread of the virus, and possible punch out the EGFP > area to get mRNA and/or protein for qPCR and western blot. Are > there any suggestions for doing this? I have access to cryostats, > sliding microtomes, and brain molds. Cutting fresh brain is > difficult, so I'd like to freeze it to section on the sliding > microtome, collect a floating section, and visualize under a > fluorescent dissecting microscope. Are there any issues with doing > this? How should I freeze the brain, and how will this affect mRNA > and protein levels? How difficult are unfixed floating sections to > deal with? Finally, can I take some sections and fix by immersion > in PFA so that I can store for longer periods of time. > > Any suggestions would be appreciated. I've had very good luck > working with perfused/fixed tissue, and would like to continue with > it, but it doesn't seem possible with the mRNA/protein endpoints. > > Thanks, > > Caroline > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 45, Issue 21 > **************************************** ------- End of Original Message ------- From vazquezr <@t> ohsu.edu Wed Aug 15 08:54:05 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Aug 15 09:03:12 2007 Subject: [Histonet] HER-2 CONTROL TISSUE BLOCK Message-ID: Who are you asking? >>> "ADESUPO ADESUYI" 8/14/2007 4:52 PM >>> Hi, Please I will appreciate it, if yo 87108.u guys can send a tissue block positive for HER-2 to me. We want to use it as control for running our HER-2 test. Hoping to hear from you guys asap. Adesupo Adesuyi 531 ORTIZ DR SE APART.D ALBUQUERQUE, NM 87108 _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Wed Aug 15 09:45:14 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Aug 15 09:48:17 2007 Subject: [Histonet] Azure B in giemsa Message-ID: Hi everyone, Could someone please tell me if azure B is a component of all giemsa stain? As far as I can tell, it is in all of them and the variations are in the other steps but I'm not sure. One of my pathologists would like to know and I want to be sure that I am giving him accurate information. Thank you - you all are such a great help! Erin From jkiernan <@t> uwo.ca Wed Aug 15 10:52:13 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Aug 15 10:52:27 2007 Subject: [Histonet] Azure B in giemsa In-Reply-To: References: Message-ID: Yes. Azure B (CI 52010) is the most important component of all traditional blood stains, even if it is not deliberately included in the dyes used to make up the mixture. Giemsa's original recipe (1902) was made from a mixture of thiazine dyes called azure I, which had azure B as a major component. In a later (1924) formulation, Giemsa used azure II, a mixture containing methylene blue, azure B and other thiazines. When pure azure B became available in the 1970s various investigators, notably P.Marshall and the late D.Wittekind and their colleagues, independently showed that the Romanowsky-Giemsa effects - purple nuclei of leukocytes, red nuclei of malaria parasites etc - required only azure B and could not be obtained with any of the other thiazine dyes included in the various mixtures for staining blood. The anionic dye in a blood stain is nearly always eosin Y. Leishman's (1901) is exceptional in having eosin B instead. There's plenty of literature in this field. The most recent review that I know is Wittekind's Chapter 21 in the 10th edition of "Conn's Biological Stains" (Oxford: BIOS, 2002, pp 303-312). There's also a very readable article called "Understanding Romanowsky Staining" by Horobin & Walter in Histochemistry 86: 331-336 (1987). The piece on azure B in the late Floyd Green's "Sigma Aldrich Handbook of Stains, Dyes and Indicators" (1990, pp 112-114 is also helpful, and includes discussion of the different available salts of pure azure B, which is made by direct synthesis rather than by degradation of methylene blue. John A. Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada N6A 5C1 http://publish.uwo.ca/~jkiernan/index.htm ____ ----- Original Message ----- From: "Martin, Erin" Date: Wednesday, August 15, 2007 10:50 Subject: [Histonet] Azure B in giemsa To: histonet > Hi everyone, > > Could someone please tell me if azure B is a component of all > giemsa stain? As far as I can tell, it is in all of them > and the variations are in the other steps but I'm not > sure. One of my pathologists would like to know and I want > to be sure that I am giving him accurate information. > > Thank you - you all are such a great help! > > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Aug 15 10:53:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 15 10:53:45 2007 Subject: [Histonet] Azure B in giemsa In-Reply-To: Message-ID: <428732.1638.qm@web61216.mail.yahoo.com> Erin: Azure I or Giemsa stain, is a "secret" proportion of mixtures of Azure A and Azure B Azure II is a mixture of Azure I (Azure A + Azure B) with methylene blue. Short answer: yes, "Giemsa" always contains Azure B By the way, "azure" is a word derived from the Persian (variation of lapis lazuly) known and used by the old Egyptians. Ren? J. "Martin, Erin" wrote: Hi everyone, Could someone please tell me if azure B is a component of all giemsa stain? As far as I can tell, it is in all of them and the variations are in the other steps but I'm not sure. One of my pathologists would like to know and I want to be sure that I am giving him accurate information. Thank you - you all are such a great help! Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From ian.montgomery <@t> bio.gla.ac.uk Wed Aug 15 11:39:00 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Aug 15 11:39:13 2007 Subject: [Histonet] Ground sections. Message-ID: <000001c7df5a$c8603f30$6424d182@IBLS.GLA.AC.UK> I've just been asked by a colleague from the Dental School if I can mount some ground sections of teeth he's discovered at the back of a cupboard. Being a simple physiologist I've never dealt with this type of material so any hints and tips as to the technique I should use would be welcome? I'm scouring the books, but why re-invent the wheel. Should I wash the sections to remove years of accumulated dust and in what, then air dry or dehydrate? Mounting media, anything that's really recommended? I had thought of Entallin but I'm open to other suggestions. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From Maria.Mejia <@t> ucsf.edu Wed Aug 15 11:41:00 2007 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Wed Aug 15 11:42:24 2007 Subject: [Histonet] quick and easy sectioning of fresh brain References: <756D65C0-7DC5-4302-A4FC-BAF3AFA8CEB2@bidmc.harvard.edu> Message-ID: <6CF686BD6F24A546B85B24FE3B97864701B78F57@EXVS06.net.ucsf.edu> Hello Caroline, You did not mention what animal tissue you'll be using. In any case, say for a large animal brain like primate why not perfuse with saline (no fixation solution), and remove the brain. Decide how thick (in microns) you want the fresh brain to be blocked e.g. 3mm. Then just take every other one tissue block and freeze using dry ice in isopentane and corretly store these blocks in -80C until ready to cut fresh frozen sections on either the sliding microtome or cryostat. You have to decide on the thickness of these sections e.g. 20ums. Then the other blocks NOT for fresh frozen sections can be placed in a fixative solution of you choice. Immersion fixation in a solution for - overnight at 4C with gentle agitation (next day check tissue to make sure its fixed - no pink color on tissue). After tissue blocks have been fixed, wash 2X in PBS (agitage) @ RT to remove residual fixative solution - 20 mins each wash. Then place these tissue blocks in 30% sucrose made in PBS - @ 4C w/agitation until the tissue sinks to the bottom of container - takes a few days. After this has been done, pad each block of excess sucrose solution with kimwips and freeze brain section blocks (see histonet archives for freezing tissue using aluminum block method & liquid nitrogen or if you are interested in this method I will provide a protocol...later). Then store each block properly in -80C freeze until ready to section for fixed free-floating sections @ 40ums. I hope this helps some. best regards Maria Bartola Mejia Department of Neurosurgery UCSF SF CA 94103 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Caroline Bass Sent: Tue 8/14/2007 9:46 PM To: Histonet Subject: [Histonet] quick and easy sectioning of fresh brain Hey Folks, So I want to have my cake and eat it too. I have injected a rat with a virus that will express EGFP. Ideally I'd like to collect the fresh brain, cut off thick floating sections, enough to get a feel for the spread of the virus, and possible punch out the EGFP area to get mRNA and/or protein for qPCR and western blot. Are there any suggestions for doing this? I have access to cryostats, sliding microtomes, and brain molds. Cutting fresh brain is difficult, so I'd like to freeze it to section on the sliding microtome, collect a floating section, and visualize under a fluorescent dissecting microscope. Are there any issues with doing this? How should I freeze the brain, and how will this affect mRNA and protein levels? How difficult are unfixed floating sections to deal with? Finally, can I take some sections and fix by immersion in PFA so that I can store for longer periods of time. Any suggestions would be appreciated. I've had very good luck working with perfused/fixed tissue, and would like to continue with it, but it doesn't seem possible with the mRNA/protein endpoints. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 15 11:51:13 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 15 11:51:26 2007 Subject: [Histonet] Ground sections. In-Reply-To: <000001c7df5a$c8603f30$6424d182@IBLS.GLA.AC.UK> Message-ID: <813951.45926.qm@web61218.mail.yahoo.com> Ian: You should first "clean" them. Wash them as thoroughly as possible, and then mount them. At that point you can either mount them on a slide dry, or if they are thin enough, either on a hydrous or anhydrous medium. Their water content is so low that you can skip a "graded dehydration", and simply place them in xylene. This will give them some "transparency" and then cover. The thing is that even if they are really thin, they are so thick for "coverslipping standards" that eventually they will collect air under the coverslip, unless you use a round one and seal them, in which case the best mounting medium would be hydrated. If you can I would advise to look for a copy of the Bolles-Lee's "Microtomist Vade Mecum", or Peter Gray's "Microtomist formulary and guide", where you can read more details on this very old technique So, as you can see, you have many options. Renj J. Ian Montgomery wrote: I've just been asked by a colleague from the Dental School if I can mount some ground sections of teeth he's discovered at the back of a cupboard. Being a simple physiologist I've never dealt with this type of material so any hints and tips as to the technique I should use would be welcome? I'm scouring the books, but why re-invent the wheel. Should I wash the sections to remove years of accumulated dust and in what, then air dry or dehydrate? Mounting media, anything that's really recommended? I had thought of Entallin but I'm open to other suggestions. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From kellerp <@t> ent.wustl.edu Wed Aug 15 12:27:00 2007 From: kellerp <@t> ent.wustl.edu (Pat Keller) Date: Wed Aug 15 12:27:19 2007 Subject: [Histonet] Cryo sections Message-ID: I am cutting adult rat brainstems, transcardially perfused with 4% paraformaldehyde in PBS and immersed in same overnight at 4c then sunk in 30 sucrose in PBS cutting at 8 microns on cryostat as they dry they rip regards of the type of slide. What in the world am I doing wrong been cutting for 25 yr. and this is a first for me. Box at -25c using high profile accu-edge blade, using both OCT and TBS no help still ripping Took sample in paraffin good cut at 8 microns Thanks Pat Keller Patricia Keller, Sr. Res. Tech. Histology Core Manager Washington University School of Medicine Department of Otolaryngology Campus box 8115 4566 Scott Ave. St. Louis, MO. 63110 KellerP@ent.wustl.edu http://www.otocore.org/ OFFICE 314-747-7166 FAX 314-747-7230
The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From pmarcum <@t> vet.upenn.edu Wed Aug 15 13:11:23 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Aug 15 13:12:20 2007 Subject: [Histonet] Region II Meeting in Delaware Sept 6, 7, 8, 2007 Message-ID: <6.2.5.6.2.20070815140901.01cad428@vet.upenn.edu> Register now!! We are accepting registration for our meeting to be held September 6,7,8, 2007 at the Delaware Technical Communtiy College in Stanton Delaware. The location is right off Interstate 95 and very easy access. We can e-mail a copy of the program if you forward your address to me ASAP. We have management seminars on Thursday, September 6th for those who need to get credits in for management CEUs and then Friday/Saturday for general histology/cytology. IF YOU NEED CEUs AND CAN NOT GET TO DENVER FOR THE NATIONAL WE HAVE A MEETING TO HELP YOU REACH YOUR GOALS FOR THE YEAR!! Any vendors who have not registered for exhibit space should contact me ASAP for booth availability. We still have some space available for you. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From gvdobbin <@t> ihis.org Wed Aug 15 13:31:09 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Aug 15 13:40:39 2007 Subject: [Histonet] Any AP users of Cerner Millenium out there? Message-ID: Hi Folks, I'm looking for any Anatomic Pathology folks that are already utilizing the Cerner LIS, Millenium version. We are tentatively scheduled to go live in November. It would be great to have someone to chat with who has already waded in to this quagmire. If you could contact me off list that would be great! Thanks in advance. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From gcallis <@t> montana.edu Wed Aug 15 14:02:59 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Aug 15 14:03:18 2007 Subject: [Histonet] Ground sections. In-Reply-To: <000001c7df5a$c8603f30$6424d182@IBLS.GLA.AC.UK> References: <000001c7df5a$c8603f30$6424d182@IBLS.GLA.AC.UK> Message-ID: <6.0.0.22.1.20070815125053.01b5d5d0@gemini.msu.montana.edu> If these are embedded in methyl methacrylate and very thick, you can superglue them to white plastic slides or even a glass slide. You can wash them off with water, blot and let air dry before gluing. If you wish, gently ultrasound them to remove the dirt, a bit of detergent (1 drop in 500 mls water) then rinse with ultrasound and clean water. If the sections want to curl, place them under smooth filter paper, and heavy weight on top to flatten. Use a weight to mount the sections too unless you use immersion oil. If the teeth are merely ground and not embedded at all, just soak them in a permanent mounting media (maybe some gradient mixtures with xylene/media) until the section is in the final thick media, then mount the section with more media. It will be messy and if the tooth is too thick, retraction of the media will occur. Just back fill, or seal in some way to retain the media, prevent retraction due to evaporation. We used to do this with hand ground, unembedded ground bone sections, and I still have them in my lab, the media a bit retracted but can be salvaged. Paleontologists mount their ground sections in other gooey stuff, but I do not recall what it is, you may be able to find that in a publication or a paleontology lab at a nearby campus. If there is still plastic surrounding the ground tooth section, do NOT use a xylene or solvent based mounting media. You can view the section after gluing onto a slide by placing a 1 1/2 thick coverglass on top, then view. It is possible to mount the coverglass with immersion oil, but it is messy for storage afterwards. To view, just turn up the light so it diffuses well IF the section is on a white plastic slide (surface staining viewing) or you can sit your clear glass slide on a white paper and do the same thing. If you use a solvent based mounting media, it will crack the plastic due to the softening of the PMMA by xylene. Have fun! At 10:39 AM 8/15/2007, you wrote: > I've just been asked by a colleague from the Dental School if I >can mount some ground sections of teeth he's discovered at the back of a >cupboard. Being a simple physiologist I've never dealt with this type of >material so any hints and tips as to the technique I should use would be >welcome? I'm scouring the books, but why re-invent the wheel. > > Should I wash the sections to remove years of accumulated dust >and in what, then air dry or dehydrate? > > Mounting media, anything that's really recommended? I had >thought of Entallin but I'm open to other suggestions. > >Ian. > > > > > >Dr. Ian Montgomery, > >Histotechnology, > >I.B.L.S. Support Unit, > >Thomson Building, > >University of Glasgow, > >G12 8QQ. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Vickroy.Jim <@t> mhsil.com Wed Aug 15 14:05:40 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Aug 15 14:05:57 2007 Subject: [Histonet] CERNER MILLLENIUM VERSUS COPATH PLUS Message-ID: <24A4826E8EF0964D86BC5317306F58A501694AA3A2@mmc-mail.ad.mhsil.com> We currently have Cerner Classic and will be switching to Cerner Millenium or Cerner CoPath Plus. Does anybody have experiences with both systems and could they give me some recommendation on which system is better and why. We process about 35,000 surgicals a year and already have Cerner Millenium for our hospital wide system. So far the pathologists are pushing for CoPath but really are not familiar with Millenium. We have Classic and either will be an improvement. Thanks Jim Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From gu.lang <@t> gmx.at Wed Aug 15 14:37:51 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Aug 15 14:38:11 2007 Subject: [Histonet] alcohol fixation image Message-ID: <000401c7df73$c57a2ce0$6412a8c0@dielangs.at> Hi, I have to admit, that I need help with basic histotechnical knowledge. Please, can someone describe me the "alcohol fixation image" of tissue, especially chromatin? And would poorly formalin-fixed tissue show less chromatin-structure or more intense chromatin-structure, after the usual VIP-processing. (50-70-96-96-100-100 alc.) due to the ethanol-effect? Thanks in advance Gudrun Lang From LuckG <@t> empirehealth.org Wed Aug 15 14:41:35 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Aug 15 14:41:49 2007 Subject: FW: [Histonet] Job Openings (P.A. x 1 + Histotech x 2) Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFE87@IRMEXCH01.irm.inhs.org> Hello all, Deaconess is a 300 bed acute care community medical center. We have two full time, M-F, day time "Histotech" positions open. Must be HT(ASCP) certified or eligible. Duties include all routine histology functions in a clinical setting (i.e. embedding , cutting, special stains and frozen sections). IHC experience is helpful but not essential. Due to two recent market adjustments by our organization the salary range for these positions has just increased 30-35% depending on years of experience. This is a golden opportunity for someone to improve on their current situation or to begin anew. Anyone new to this field is encouraged to apply as well, for the door is wide open. The "Pathologist's Assistant" position is a brand new addition to our daily operational staff. We have a grossing suite attached to surgery for which this individual will be in charge of and provides an independent atmosphere and virtual control over the work environment and offers a very competitive wage and benefit package. The position will be M-F days with some shared call with me for nights and weekends. I suggest you apply on-line by going to our corporate website www.empirehealth.org. The position is at Deaconess Medical Center in Spokane, WA www.deaconess-spokane.org. Please feel free to contact me directly for more details or assistance with the on-line application process. Following is an additional web link to provide you with more info on Spokane and the greater Inland Northwest region www.visitspokane.com. ("Near Perfect-Near Nature"). Thank you and best wishes, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconess-spokane.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbanwait <@t> buckinstitute.org Wed Aug 15 14:47:56 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Wed Aug 15 14:48:08 2007 Subject: [Histonet] embryo sections Message-ID: Hi there in histoland... someone just gave me a phone call asking about a previous embryo posting from about a year back.... I found an old contact that may be able to help you... apologies, I did not get your name or number... I hope you are reading this so I can pass along the info! send me an email for the info. Cheers, -Surita ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate III Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From agustinvictor <@t> gmail.com Wed Aug 15 15:37:22 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Wed Aug 15 15:37:34 2007 Subject: [Histonet] Problems STP 120 Spin Tissue Processor Message-ID: <00b101c7df7c$186046d0$590215ac@Pentium4> In the laboratory we use a STP 120 Spin Tissue Processor. Sometimes, for reasons that nobody knows, the first paraffin containers appears solidified. The basket with biopsies is outside, in the high, several hours until I arrive in the morning. The technical service has not known how to solve it. Has somebody had problems with this model? Ht Agustin Victor Chertcoff Gastroeonterology Municipal Hospital Buenos Aires Argentina From agustinvictor <@t> gmail.com Wed Aug 15 20:05:51 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Wed Aug 15 20:05:52 2007 Subject: [Histonet] Problems STP 120 Spin Tissue Processor References: <25166064.8746831187213643966.JavaMail.root@vms061.mailsrvcs.net> Message-ID: <006801c7dfa1$99ada050$590215ac@Pentium4> thanks to all for their answers. I will see if I can sleep tonight! Agustin ----- Original Message ----- From: To: "Agustin Chertcoff" Sent: Wednesday, August 15, 2007 6:34 PM Subject: Re: [Histonet] Problems STP 120 Spin Tissue Processor > Hi Augustin, > > We had one for a few months in early 2006 and it was a nightmare. I never > had your experience, but yes, it would do things that were so outside the > norm, that we couldn't trust it. They took it away and we got a new VIP5 > for conventional processing and in the Fall 2006 also got 2 RHS1 microwave > processors that are a dream. > > We had no luck with service on the 120, just like you. Be very careful > with this instrument. I wouldn't trust it. > > Good Luck > > Eileen Lonergan > Manager > Histology > converge Diagnostic services > Peabody MA > > (978) 548-5206 > > >>From: Agustin Chertcoff >>Date: 2007/08/15 Wed PM 03:37:22 CDT >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] Problems STP 120 Spin Tissue Processor > >>In the laboratory we use a STP 120 Spin Tissue Processor. Sometimes, for >>reasons that nobody knows, the first paraffin containers appears >>solidified. The basket with biopsies is outside, in the high, several >>hours until I arrive in the morning. The technical service has not known >>how to solve it. Has somebody had problems with this model? >> >>Ht Agustin Victor Chertcoff >>Gastroeonterology Municipal Hospital >>Buenos Aires >>Argentina >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Eileen Lonergan > (207) 749-9617 cell > (207) 324-6468 home From asachau <@t> titanmed.com Thu Aug 16 10:26:09 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Thu Aug 16 10:28:04 2007 Subject: [Histonet] Position Available - VACATION DESTINATION! In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7B5870C@titansbs1.corp.titanmed.com> Hello Histo-netters! I have Histology need in a Tropical Area. Please contact me if you are interested/available. ASCP certification is required. It is a 13 week assignment/40 hour work week. Housing (Resort), flight, rental car provided. April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From timothy.m.coskran <@t> pfizer.com Thu Aug 16 14:42:12 2007 From: timothy.m.coskran <@t> pfizer.com (Coskran, Timothy M) Date: Thu Aug 16 14:42:35 2007 Subject: [Histonet] detecting polyethylene glycol Message-ID: <3F4063B77CC1F445933078B93A0A0C720311D4D4@groamrexm05.amer.pfizer.com> Does anyone know of a histochemical or IHC method for detecting polyethylene glycol in tissue samples? Thanks, Tim Coskran DSRD-Groton ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From jimr0712 <@t> comcast.net Thu Aug 16 16:39:54 2007 From: jimr0712 <@t> comcast.net (jimr0712@comcast.net) Date: Thu Aug 16 16:40:05 2007 Subject: [Histonet] Question about CLIA requirements Message-ID: <081620072139.27209.46C4C42A0007B15200006A492216554886CDCEC9CF9D030706@comcast.net> The IT staff at a hospital that we service, stated that CLIA requires the computer hardware to be under a CLIA license. Is this true ? Have not heard this before. Thanks. -- Jim Robinson, M.S., HTL/HT (ASCP) Director of Laboratory Operations Orizon Diagnostics, LLC 102 Chestnut Avenue Westmont, Illinois 60559 jrobinson@orizondiagnostics.com 630-321-1506 (fax) 630-230-6325 From jnocito <@t> satx.rr.com Thu Aug 16 17:30:12 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 16 17:30:38 2007 Subject: [Histonet] Question about CLIA requirements References: <081620072139.27209.46C4C42A0007B15200006A492216554886CDCEC9CF9D030706@comcast.net> Message-ID: <000b01c7e055$03717e40$d49eae18@yourxhtr8hvc4p> This is news to me and I just had a CLIA inspection last month. Joe ----- Original Message ----- From: To: "Histonet2" ; "Histonet1" ; "Histonet" Sent: Thursday, August 16, 2007 4:39 PM Subject: [Histonet] Question about CLIA requirements > The IT staff at a hospital that we service, stated that CLIA requires the > computer hardware to be under a CLIA license. Is this true ? Have not > heard this before. > > Thanks. > > -- > Jim Robinson, M.S., HTL/HT (ASCP) > Director of Laboratory Operations > Orizon Diagnostics, LLC > 102 Chestnut Avenue > Westmont, Illinois 60559 > jrobinson@orizondiagnostics.com > 630-321-1506 (fax) > 630-230-6325 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pereirafamily <@t> cox.net Thu Aug 16 18:05:42 2007 From: pereirafamily <@t> cox.net (pereirafamily) Date: Thu Aug 16 18:05:58 2007 Subject: [Histonet] prostate needle biopsies Message-ID: <000601c7e059$f8a40b40$09650744@pereirafarm> Is anyone using anything other than sponges in their cassettes for prostate needle biopsies? If so, do you find that it works better? From tkngflght <@t> yahoo.com Thu Aug 16 19:10:51 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Aug 16 19:10:27 2007 Subject: an easy solution RE: [Histonet] prostate needle biopsies In-Reply-To: <000601c7e059$f8a40b40$09650744@pereirafarm> Message-ID: <006a01c7e063$12e992f0$6701a8c0@CHERYLSLAPTOP> You'll laugh--but this works GREAT. On top of the sponges a couple of labs I've worked at used white C-fold paper towels cut to size to lay against the biopsies. They process well, lift RIGHT OFF without sticking as long as the toweling was damp when the needle bxs were laid on them. No trebeculation from the sponges--and they were FLAT. It would layer like this: cassette, damp sponge, cut paper towel, biopsy(ies), cut paper towel, damp sponge, cassette lid. If you wanted to dye them squirt eosin or merchurichrome on the cassette once closed and they stain. Amazing-and pretty much free. It works with EMB and other 'sticky' stuff like FNA buttons. Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 800.756.3309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pereirafamily Sent: Thursday, August 16, 2007 6:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate needle biopsies Is anyone using anything other than sponges in their cassettes for prostate needle biopsies? If so, do you find that it works better? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.reilly <@t> jcu.edu.au Fri Aug 17 02:05:49 2007 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Aug 17 02:05:58 2007 Subject: [Histonet] Sensitivity and specificity Message-ID: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> Histonetters, Can anyone help with a concise definition of "sensitivity" and "specificity" as it relates to immunohistochemical reactions. Thanks and regards, Laurie. Mr. Laurie Reilly School of Veterinary & Biomedical Sciences James Cook University Townsville Queensland 4811 Australia Phone 07 4781 4468 Fax 07 4779 1526 From gu.lang <@t> gmx.at Fri Aug 17 03:02:25 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 17 03:02:34 2007 Subject: AW: [Histonet] Sensitivity and specificity In-Reply-To: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> Message-ID: <000901c7e0a4$f360e360$6412a8c0@dielangs.at> Sensitivity refers to the minimal ammount of antigen, that can be detected by the whole detection-system (primary antibody qualitiy/quantity; secondary antibody; activity of the used enzyme; etc.) The sensitivity can be encreased by higher affine antibodies, more "steps", amplification. The specifity of a test refers to the fact, that the test is only positiv, if the tested substance is really present; the test must not be positiv with any other substance. For example, if an antibody crossreacts with epitops on various celltypes, the antibody and the test are not specific for a unique cell-type. - but the antibody itself is specific to its epitop. Hope this helps Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Laurie Reilly Gesendet: Freitag, 17. August 2007 09:06 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sensitivity and specificity Histonetters, Can anyone help with a concise definition of "sensitivity" and "specificity" as it relates to immunohistochemical reactions. Thanks and regards, Laurie. Mr. Laurie Reilly School of Veterinary & Biomedical Sciences James Cook University Townsville Queensland 4811 Australia Phone 07 4781 4468 Fax 07 4779 1526 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Fri Aug 17 05:21:27 2007 From: kemlo <@t> f2s.com (kemlo) Date: Fri Aug 17 05:22:02 2007 Subject: [Histonet] Sensitivity and specificity In-Reply-To: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> References: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> Message-ID: <9453D8E8B8814677AA84C02BD8049AD9@KemloPC> Doesn't sensitivity mean that it will stain all of 'it', even that which isn't 'it'. Specificity means that it only stains 'it' but sometimes not all of 'it'. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Reilly Sent: 17 August 2007 08:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sensitivity and specificity Histonetters, Can anyone help with a concise definition of "sensitivity" and "specificity" as it relates to immunohistochemical reactions. Thanks and regards, Laurie. Mr. Laurie Reilly School of Veterinary & Biomedical Sciences James Cook University Townsville Queensland 4811 Australia Phone 07 4781 4468 Fax 07 4779 1526 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Fri Aug 17 05:25:09 2007 From: kemlo <@t> f2s.com (kemlo) Date: Fri Aug 17 05:25:41 2007 Subject: [Histonet] detecting polyethylene glycol In-Reply-To: <3F4063B77CC1F445933078B93A0A0C720311D4D4@groamrexm05.amer.pfizer.com> References: <3F4063B77CC1F445933078B93A0A0C720311D4D4@groamrexm05.amer.pfizer.com> Message-ID: <7F60092C0C774263B3EA2CD1855BFEC5@KemloPC> Won't polyethylene glycol wash out? You won't be able to process it so I guess it's frozen sections, but they won't freeze if there's polyethylene glycol present will they? Unless it gets broken down into a metabolite then I'm at a loss. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coskran, Timothy M Sent: 16 August 2007 20:42 To: Histonet@pathology.swmed.edu Subject: [Histonet] detecting polyethylene glycol Does anyone know of a histochemical or IHC method for detecting polyethylene glycol in tissue samples? Thanks, Tim Coskran DSRD-Groton ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 17 08:10:51 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 08:10:55 2007 Subject: an easy solution RE: [Histonet] prostate needle biopsies In-Reply-To: <006a01c7e063$12e992f0$6701a8c0@CHERYLSLAPTOP> Message-ID: <104128.57928.qm@web61215.mail.yahoo.com> You even could do WITHOUT the sponges, just the paper towel. Ren? J. "Cheryl R. Kerry" wrote: You'll laugh--but this works GREAT. On top of the sponges a couple of labs I've worked at used white C-fold paper towels cut to size to lay against the biopsies. They process well, lift RIGHT OFF without sticking as long as the toweling was damp when the needle bxs were laid on them. No trebeculation from the sponges--and they were FLAT. It would layer like this: cassette, damp sponge, cut paper towel, biopsy(ies), cut paper towel, damp sponge, cassette lid. If you wanted to dye them squirt eosin or merchurichrome on the cassette once closed and they stain. Amazing-and pretty much free. It works with EMB and other 'sticky' stuff like FNA buttons. Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 800.756.3309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pereirafamily Sent: Thursday, August 16, 2007 6:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate needle biopsies Is anyone using anything other than sponges in their cassettes for prostate needle biopsies? If so, do you find that it works better? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From karenadams <@t> comcast.net Fri Aug 17 08:11:47 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Fri Aug 17 08:11:52 2007 Subject: [Histonet] Chattanooga area.... Message-ID: <081720071311.28975.46C59E93000B65950000712F22070215539C030E0B0E020A9D0E05@comcast.net> Hello histonetters.....if anyone is on the server that works in or near the Chattanooga area could you please write me back.....Thank you! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From Jackie.O'Connor <@t> abbott.com Fri Aug 17 08:19:55 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Aug 17 08:20:35 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: <81196.87505.qm@web50301.mail.re2.yahoo.com> Message-ID: Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 From rebecca.jones256 <@t> gmail.com Fri Aug 17 08:57:58 2007 From: rebecca.jones256 <@t> gmail.com (Rebecca Jones) Date: Fri Aug 17 08:58:03 2007 Subject: [Histonet] Xylene Substitute Testing Results Message-ID: Hi all! Earlier this summer I asked many of you your opinion on a good xylene substitute to use. I got great suggestions and tips from all of you. I decided to test two, Clear Rite and Formula 83. Both performed well, but the Formula 83 has won the test. It performed most like xylene and required little to no changes in my procedures. I did choose Richard Allan Mounting Media, as it worked great with the Formula 83 and was suggested by the CBG rep. The mounting media I was using was not compatible with the sub. Formula 83 did dry slides slightly faster as they came off the stain line, but the slide quality still performed to the boss man's liking. Thank you all for your input. Back to work I go. Rebecca From gu.lang <@t> gmx.at Fri Aug 17 09:08:53 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 17 09:09:01 2007 Subject: AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: Message-ID: <000801c7e0d8$24e36ab0$6412a8c0@dielangs.at> Embalming is no usual procedure in Austria. Perhaps that's the cause, why there wasn't any comment on this issue in the media. And the funeral homes are quiet institutions.... Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jackie M O'Connor Gesendet: Freitag, 17. August 2007 15:20 An: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Betreff: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 17 09:09:04 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 09:09:08 2007 Subject: [Histonet] Sensitivity and specificity In-Reply-To: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> Message-ID: <269809.68833.qm@web61221.mail.yahoo.com> Laurie: Both Gudrun and Kemlo are right, let me just try to put it in other way: 1-specificity: your detection system will react ONLY with the targeted component (it is, after all, an antigen/antibody reaction based on the quaternary protein structure of both), and 2-sensitivity: your detection system will detect ALL of the tageted component, even in its lowest concentrations. The more sensitive your detection system is, the less amount of the tageted compound it will be able to detect. Ren? J. Laurie Reilly wrote: Histonetters, Can anyone help with a concise definition of "sensitivity" and "specificity" as it relates to immunohistochemical reactions. Thanks and regards, Laurie. Mr. Laurie Reilly School of Veterinary & Biomedical Sciences James Cook University Townsville Queensland 4811 Australia Phone 07 4781 4468 Fax 07 4779 1526 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. From rjbuesa <@t> yahoo.com Fri Aug 17 09:11:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 09:12:25 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: Message-ID: <88243.841.qm@web61225.mail.yahoo.com> Not from Europe, but we just should follow their example! Ren? J. Jackie M O'Connor wrote: Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From kemlo <@t> f2s.com Fri Aug 17 09:25:41 2007 From: kemlo <@t> f2s.com (kemlo) Date: Fri Aug 17 09:26:18 2007 Subject: [Histonet] Sensitivity and specificity In-Reply-To: <269809.68833.qm@web61221.mail.yahoo.com> References: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> <269809.68833.qm@web61221.mail.yahoo.com> Message-ID: I said it more succinctly . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 17 August 2007 15:09 To: Laurie Reilly; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sensitivity and specificity Laurie: Both Gudrun and Kemlo are right, let me just try to put it in other way: 1-specificity: your detection system will react ONLY with the targeted component (it is, after all, an antigen/antibody reaction based on the quaternary protein structure of both), and 2-sensitivity: your detection system will detect ALL of the tageted component, even in its lowest concentrations. The more sensitive your detection system is, the less amount of the tageted compound it will be able to detect. Ren? J. Laurie Reilly wrote: Histonetters, Can anyone help with a concise definition of "sensitivity" and "specificity" as it relates to immunohistochemical reactions. Thanks and regards, Laurie. Mr. Laurie Reilly School of Veterinary & Biomedical Sciences James Cook University Townsville Queensland 4811 Australia Phone 07 4781 4468 Fax 07 4779 1526 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Aug 17 09:27:29 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Aug 17 09:28:48 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: References: <81196.87505.qm@web50301.mail.re2.yahoo.com> Message-ID: Apparently the recent accession of Romania to the EEC, which of course includes Transylvania has been the main stakeholders in this legislation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 17 August 2007 14:20 To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Fri Aug 17 09:47:46 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Aug 17 09:47:58 2007 Subject: [Histonet] prostate needle biopsies In-Reply-To: <000601c7e059$f8a40b40$09650744@pereirafarm> References: <000601c7e059$f8a40b40$09650744@pereirafarm> Message-ID: <002c01c7e0dd$94e37f80$3402a8c0@plab.local> We love the blue sponges. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pereirafamily Sent: Thursday, August 16, 2007 6:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate needle biopsies Is anyone using anything other than sponges in their cassettes for prostate needle biopsies? If so, do you find that it works better? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From BWeston <@t> BarbHosp.com Fri Aug 17 10:01:18 2007 From: BWeston <@t> BarbHosp.com (Weston, Bernadette) Date: Fri Aug 17 10:01:25 2007 Subject: [Histonet] wikipedia Message-ID: <5213EA72AB13584EBD29CE54E1A66F0502954233@CPRTEVS02.triadhospitals.net> Not to cast doubt but are we sure Wikipedia has got this right? They have been called questioned recently. Bernadette Weston HT Histology Supervisor Barberton Citizens Hospital 330 615 3980 bweston@barbhosp.com From b-frederick <@t> northwestern.edu Fri Aug 17 10:04:43 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Aug 17 10:05:09 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: Message-ID: <000001c7e0df$f4ba3000$d00f7ca5@lurie.northwestern.edu> You mean there is a clone of Joe in the U.K.? Love it!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, August 17, 2007 9:27 AM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Apparently the recent accession of Romania to the EEC, which of course includes Transylvania has been the main stakeholders in this legislation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 17 August 2007 14:20 To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Aug 17 11:09:54 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Aug 17 10:14:06 2007 Subject: {SPAM?} RE: [Histonet] prostate needle biopsies In-Reply-To: <002c01c7e0dd$94e37f80$3402a8c0@plab.local> Message-ID: Ask your processor if it loves the blue sponges. :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, August 17, 2007 9:48 AM To: 'pereirafamily'; histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] prostate needle biopsies We love the blue sponges. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pereirafamily Sent: Thursday, August 16, 2007 6:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate needle biopsies Is anyone using anything other than sponges in their cassettes for prostate needle biopsies? If so, do you find that it works better? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mary.ann.deathridge <@t> Vanderbilt.Edu Fri Aug 17 10:12:39 2007 From: mary.ann.deathridge <@t> Vanderbilt.Edu (Deathridge, Mary Ann) Date: Fri Aug 17 10:17:37 2007 Subject: [Histonet] job postings Message-ID: <113162616B6440479A211712490222C684C4CA@mailbe10.mc.vanderbilt.edu> Vanderbilt University Medical Center, Nashville, TN. has (2) two histotechnologist (ASCP) position openings in the histopathology lab. We are looking for techs with immunohistochemistry experience, as well as, general histology skills. VUMC offers a great benefits package and a highly competitive salary range in line with today's market. Vanderbilt prides itself in being one of the best places to work with high employee satisfaction. Check out the web site at www.mc.vanderbilt.edu go to HR, available jobs, to post resume. Feel free to contact me for further information. MaryAnn Deathridge, BS., HT (ASCP) Supervisor, Histopathology (615) 343-7012 From denise.woodward <@t> uconn.edu Fri Aug 17 10:26:11 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri Aug 17 10:26:28 2007 Subject: [Histonet] B&B Gram negative color change In-Reply-To: <5213EA72AB13584EBD29CE54E1A66F0502954233@CPRTEVS02.triadhospitals.net> References: <5213EA72AB13584EBD29CE54E1A66F0502954233@CPRTEVS02.triadhospitals.net> Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C401662FC@EXCHANGED.mgmt.ad.uconn.edu> Hello all, We recently noticed that our B&B positive controls change color over time after staining. It happens in as little time as a week. The Gram negative organisms change hue from a nice pink/red to a purple color. The Gram positive organisms remain blue. Anyone have any clues as to why/how this is happening? Sorry to ask a difficult question on a Friday but this one has been bothering me for a few days. Thanks, Denise Long Woodward UConn Dept. of Pathobiology and Veterinary Sciences Storrs, CT From contact <@t> excaliburpathology.com Fri Aug 17 10:28:13 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Aug 17 10:28:24 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Message-ID: <171741.71965.qm@web50111.mail.re2.yahoo.com> So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT From gmmunoz <@t> prodigy.net Fri Aug 17 10:36:50 2007 From: gmmunoz <@t> prodigy.net (GLORIA MUNOZ) Date: Fri Aug 17 10:37:07 2007 Subject: [Histonet] microwave reproducibility Message-ID: <975935.62550.qm@web80206.mail.mud.yahoo.com> Hi HistoNetters: Just wondering if any of you have a procedure to share on microwave reproducibility for a store-bought, non-medical microwave. We had our CAP inspection recently and were asked to come up with a method to test the microwave. The inspector recommended heating distilled water for one minute at each of the three different power levels and documenting the results. Does anyone know of another, maybe better, way? Thanks a lot, enjoy reading your entries, and hope you all have a restful weekend...........gloria From rjbuesa <@t> yahoo.com Fri Aug 17 10:55:41 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 10:55:53 2007 Subject: [Histonet] B&B Gram negative color change In-Reply-To: <40AC6D73C2B95C4CA21B26B7BF380C401662FC@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: <60752.31377.qm@web61217.mail.yahoo.com> If you do not totally wash/remove/differentiate between steps, that could happen. Ren? J. "Woodward, Denise" wrote: Hello all, We recently noticed that our B&B positive controls change color over time after staining. It happens in as little time as a week. The Gram negative organisms change hue from a nice pink/red to a purple color. The Gram positive organisms remain blue. Anyone have any clues as to why/how this is happening? Sorry to ask a difficult question on a Friday but this one has been bothering me for a few days. Thanks, Denise Long Woodward UConn Dept. of Pathobiology and Veterinary Sciences Storrs, CT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From rjbuesa <@t> yahoo.com Fri Aug 17 10:57:12 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 10:57:24 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: <171741.71965.qm@web50111.mail.re2.yahoo.com> Message-ID: <839201.66477.qm@web61216.mail.yahoo.com> Remember how the Transilvanian "undeads"increase in numer by neck bitting/blood sucking. Ren? J. Paula Pierce wrote: So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From rjbuesa <@t> yahoo.com Fri Aug 17 10:58:55 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 10:59:07 2007 Subject: [Histonet] microwave reproducibility In-Reply-To: <975935.62550.qm@web80206.mail.mud.yahoo.com> Message-ID: <946594.66477.qm@web61211.mail.yahoo.com> Follow your inspector's suggestion. That is very good way "to prove" reproducibility. Ren? J. GLORIA MUNOZ wrote: Hi HistoNetters: Just wondering if any of you have a procedure to share on microwave reproducibility for a store-bought, non-medical microwave. We had our CAP inspection recently and were asked to come up with a method to test the microwave. The inspector recommended heating distilled water for one minute at each of the three different power levels and documenting the results. Does anyone know of another, maybe better, way? Thanks a lot, enjoy reading your entries, and hope you all have a restful weekend...........gloria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From KHilburn <@t> compucyte.com Fri Aug 17 11:10:38 2007 From: KHilburn <@t> compucyte.com (Kate Hilburn) Date: Fri Aug 17 11:09:59 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: <171741.71965.qm@web50111.mail.re2.yahoo.com> Message-ID: I would think the Transylvanian vampires would be far more concerned about AIDS than cancer. Kate Hilburn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, August 17, 2007 11:28 AM To: Histonet Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprin119 <@t> umn.edu Fri Aug 17 11:25:29 2007 From: sprin119 <@t> umn.edu (Jennifer Springsteen) Date: Fri Aug 17 11:26:40 2007 Subject: [Histonet] Thermo Shandon Excelsior tissue processor In-Reply-To: References: Message-ID: <4f3f3ed37712d58e2434149799c80f38@umn.edu> Does anyone use a Thermo Shandon Excelsior tissue processor? We just bought a new one, but found that the "state-of-the-art" molded polymer chamber has small cracks at some of the seams, which would make reagent containment and vacuum processing a bit difficult. Thermo is sending someone to assess/replace the unit, but I want to know if anyone else has experience with the machine to see if this is a common problem? Has anyone ever seen cracks develop after repeated use? Any idea whether the Shandon Pathcentre processor is better? Thanks! -Jennifer Springsteen, Lab Manager Lillehei Heart Institute Histopathology & Microscopy Core University of Minnesota School of Medicine Minneapolis, MN 55455 On Aug 17, 2007, at 11:10 AM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. detecting polyethylene glycol (Coskran, Timothy M) > 2. Question about CLIA requirements (jimr0712@comcast.net) > 3. Re: Question about CLIA requirements (Joe Nocito) > 4. prostate needle biopsies (pereirafamily) > 5. an easy solution RE: [Histonet] prostate needle biopsies > (Cheryl R. Kerry) > 6. Sensitivity and specificity (Laurie Reilly) > 7. AW: [Histonet] Sensitivity and specificity (Gudrun Lang) > 8. RE: Sensitivity and specificity (kemlo) > 9. RE: detecting polyethylene glycol (kemlo) > 10. Re: an easy solution RE: [Histonet] prostate needle biopsies > (Rene J Buesa) > 11. Chattanooga area.... (karenadams@comcast.net) > 12. Ban on Formaldehyde in Europe deadline 22SEP07 (Jackie M > O'Connor) > 13. Xylene Substitute Testing Results (Rebecca Jones) > 14. AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > (Gudrun Lang) > 15. Re: Sensitivity and specificity (Rene J Buesa) > 16. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Rene J Buesa) > 17. RE: Sensitivity and specificity (kemlo) > 18. RE: Ban on Formaldehyde in Europe deadline 22SEP07 (Edwards, > R.E.) > 19. RE: prostate needle biopsies (Cheri Miller) > 20. wikipedia (Weston, Bernadette) > 21. RE: Ban on Formaldehyde in Europe deadline 22SEP07 > (Bernice Frederick) > 22. RE: {SPAM?} RE: [Histonet] prostate needle biopsies > (Douglas D Deltour) > 23. job postings (Deathridge, Mary Ann) > 24. B&B Gram negative color change (Woodward, Denise) > 25. Ban on Formaldehyde in Europe deadline 22SEP07 (Paula Pierce) > 26. microwave reproducibility (GLORIA MUNOZ) > 27. Re: B&B Gram negative color change (Rene J Buesa) > 28. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 16 Aug 2007 15:42:12 -0400 > From: "Coskran, Timothy M" > Subject: [Histonet] detecting polyethylene glycol > To: > Message-ID: > <3F4063B77CC1F445933078B93A0A0C720311D4D4@groamrexm05.amer.pfizer.com> > Content-Type: text/plain; charset="US-ASCII" > > Does anyone know of a histochemical or IHC method for detecting > polyethylene glycol in tissue samples? > > > > Thanks, > > Tim Coskran > > DSRD-Groton > > ---------------------------------------------------------------------- > LEGAL NOTICE > Unless expressly stated otherwise, this message is confidential and > may be privileged. It is intended for the addressee(s) only. Access > to this E-mail by anyone else is unauthorized. If you are not an > addressee, any disclosure or copying of the contents of this E-mail or > any action taken (or not taken) in reliance on it is unauthorized and > may be unlawful. If you are not an addressee, please inform the > sender immediately. > > ------------------------------ > > Message: 2 > Date: Thu, 16 Aug 2007 21:39:54 +0000 > From: jimr0712@comcast.net > Subject: [Histonet] Question about CLIA requirements > To: histonet@lists.utsouthwestern.edu (Histonet2), > histonet-request@lists.utsouthwestern.edu (Histonet1), > histonet-request@lists.utsouthwestern.edu (Histonet) > Message-ID: > > <081620072139.27209.46C4C42A0007B15200006A492216554886CDCEC9CF9D030706@ > comcast.net> > > > The IT staff at a hospital that we service, stated that CLIA requires > the computer hardware to be under a CLIA license. Is this true ? Have > not heard this before. > > Thanks. > > -- > Jim Robinson, M.S., HTL/HT (ASCP) > Director of Laboratory Operations > Orizon Diagnostics, LLC > 102 Chestnut Avenue > Westmont, Illinois 60559 > jrobinson@orizondiagnostics.com > 630-321-1506 (fax) > 630-230-6325 > > > > ------------------------------ > > Message: 3 > Date: Thu, 16 Aug 2007 17:30:12 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Question about CLIA requirements > To: , "Histonet2" > , "Histonet1" > > Message-ID: <000b01c7e055$03717e40$d49eae18@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="Windows-1252"; > reply-type=original > > This is news to me and I just had a CLIA inspection last month. > > Joe > ----- Original Message ----- > From: > To: "Histonet2" ; "Histonet1" > ; "Histonet" > > Sent: Thursday, August 16, 2007 4:39 PM > Subject: [Histonet] Question about CLIA requirements > > >> The IT staff at a hospital that we service, stated that CLIA requires >> the >> computer hardware to be under a CLIA license. Is this true ? Have not >> heard this before. >> >> Thanks. >> >> -- >> Jim Robinson, M.S., HTL/HT (ASCP) >> Director of Laboratory Operations >> Orizon Diagnostics, LLC >> 102 Chestnut Avenue >> Westmont, Illinois 60559 >> jrobinson@orizondiagnostics.com >> 630-321-1506 (fax) >> 630-230-6325 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Thu, 16 Aug 2007 16:05:42 -0700 > From: "pereirafamily" > Subject: [Histonet] prostate needle biopsies > To: > Message-ID: <000601c7e059$f8a40b40$09650744@pereirafarm> > Content-Type: text/plain; charset="iso-8859-1" > > Is anyone using anything other than sponges in their cassettes for > prostate needle biopsies? If so, do you find that it works better? > > ------------------------------ > > Message: 5 > Date: Thu, 16 Aug 2007 19:10:51 -0500 > From: "Cheryl R. Kerry" > Subject: an easy solution RE: [Histonet] prostate needle biopsies > To: "'pereirafamily'" , > > Message-ID: <006a01c7e063$12e992f0$6701a8c0@CHERYLSLAPTOP> > Content-Type: text/plain; charset="US-ASCII" > > You'll laugh--but this works GREAT. On top of the sponges a couple > of labs > I've worked at used white C-fold paper towels cut to size to lay > against the > biopsies. They process well, lift RIGHT OFF without sticking as long > as the > toweling was damp when the needle bxs were laid on them. No > trebeculation > from the sponges--and they were FLAT. > > It would layer like this: > > cassette, > damp sponge, > cut paper towel, > biopsy(ies), > cut paper towel, > damp sponge, > cassette lid. > > If you wanted to dye them squirt eosin or merchurichrome on the > cassette > once closed and they stain. > > Amazing-and pretty much free. It works with EMB and other 'sticky' > stuff > like FNA buttons. > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one great tech at a time. > 800.756.3309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Fri, 17 Aug 2007 17:05:49 +1000 > From: Laurie Reilly > Subject: [Histonet] Sensitivity and specificity > To: > Message-ID: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> > Content-Type: text/plain; charset="us-ascii" > > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > > > ------------------------------ > > Message: 7 > Date: Fri, 17 Aug 2007 10:02:25 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Sensitivity and specificity > To: "'Laurie Reilly'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <000901c7e0a4$f360e360$6412a8c0@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Sensitivity refers to the minimal ammount of antigen, that can be > detected > by the whole detection-system (primary antibody qualitiy/quantity; > secondary > antibody; activity of the used enzyme; etc.) > The sensitivity can be encreased by higher affine antibodies, more > "steps", > amplification. > > The specifity of a test refers to the fact, that the test is only > positiv, > if the tested substance is really present; the test must not be > positiv with > any other substance. For example, if an antibody crossreacts with > epitops on > various celltypes, the antibody and the test are not specific for a > unique > cell-type. - but the antibody itself is specific to its epitop. > > Hope this helps > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Laurie > Reilly > Gesendet: Freitag, 17. August 2007 09:06 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Sensitivity and specificity > > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 8 > Date: Fri, 17 Aug 2007 11:21:27 +0100 > From: "kemlo" > Subject: RE: [Histonet] Sensitivity and specificity > To: "'Laurie Reilly'" , > > Message-ID: <9453D8E8B8814677AA84C02BD8049AD9@KemloPC> > Content-Type: text/plain; charset="us-ascii" > > Doesn't sensitivity mean that it will stain all of 'it', even that > which > isn't 'it'. Specificity means that it only stains 'it' but sometimes > not all > of 'it'. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: 17 August 2007 08:06 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sensitivity and specificity > > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 9 > Date: Fri, 17 Aug 2007 11:25:09 +0100 > From: "kemlo" > Subject: RE: [Histonet] detecting polyethylene glycol > To: "'Coskran, Timothy M'" , > > Message-ID: <7F60092C0C774263B3EA2CD1855BFEC5@KemloPC> > Content-Type: text/plain; charset="us-ascii" > > Won't polyethylene glycol wash out? You won't be able to process it so > I > guess it's frozen sections, but they won't freeze if there's > polyethylene > glycol present will they? > > Unless it gets broken down into a metabolite then I'm at a loss. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Coskran, > Timothy M > Sent: 16 August 2007 20:42 > To: Histonet@pathology.swmed.edu > Subject: [Histonet] detecting polyethylene glycol > > Does anyone know of a histochemical or IHC method for detecting > polyethylene glycol in tissue samples? > > > > Thanks, > > Tim Coskran > > DSRD-Groton > > ---------------------------------------------------------------------- > LEGAL NOTICE > Unless expressly stated otherwise, this message is confidential and > may be > privileged. It is intended for the addressee(s) only. Access to this > E-mail by anyone else is unauthorized. If you are not an addressee, > any > disclosure or copying of the contents of this E-mail or any action > taken (or > not taken) in reliance on it is unauthorized and may be unlawful. If > you > are not an addressee, please inform the sender immediately. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 10 > Date: Fri, 17 Aug 2007 06:10:51 -0700 (PDT) > From: Rene J Buesa > Subject: Re: an easy solution RE: [Histonet] prostate needle biopsies > To: "Cheryl R. Kerry" , 'pereirafamily' > , histonet@lists.utsouthwestern.edu > Message-ID: <104128.57928.qm@web61215.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > You even could do WITHOUT the sponges, just the paper towel. > Ren? J. > > "Cheryl R. Kerry" wrote: > You'll laugh--but this works GREAT. On top of the sponges a couple > of labs > I've worked at used white C-fold paper towels cut to size to lay > against the > biopsies. They process well, lift RIGHT OFF without sticking as long > as the > toweling was damp when the needle bxs were laid on them. No > trebeculation > from the sponges--and they were FLAT. > > It would layer like this: > > cassette, > damp sponge, > cut paper towel, > biopsy(ies), > cut paper towel, > damp sponge, > cassette lid. > > If you wanted to dye them squirt eosin or merchurichrome on the > cassette > once closed and they stain. > > Amazing-and pretty much free. It works with EMB and other 'sticky' > stuff > like FNA buttons. > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one great tech at a time. > 800.756.3309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Park yourself in front of a world of choices in alternative vehicles. > Visit the Yahoo! Auto Green Center. > > ------------------------------ > > Message: 11 > Date: Fri, 17 Aug 2007 13:11:47 +0000 > From: karenadams@comcast.net > Subject: [Histonet] Chattanooga area.... > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <081720071311.28975.46C59E93000B65950000712F22070215539C030E0B0E020A9D0 > E05@comcast.net> > > Content-Type: text/plain > > Hello histonetters.....if anyone is on the server that works in or > near the Chattanooga area could you please write me back.....Thank > you! > > -- > Karen Adams > Pathology Laboratories West > 9303 Park West Blvd > Knoxville, TN 37923 > (865) 690-2111 FAX (865) 691-1623 > > ------------------------------ > > Message: 12 > Date: Fri, 17 Aug 2007 08:19:55 -0500 > From: "Jackie M O'Connor" > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > > ------------------------------ > > Message: 13 > Date: Fri, 17 Aug 2007 08:57:58 -0500 > From: "Rebecca Jones" > Subject: [Histonet] Xylene Substitute Testing Results > To: histonet > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all! > > Earlier this summer I asked many of you your opinion on a good xylene > substitute to use. I got great suggestions and tips from all of you. > I > decided to test two, Clear Rite and Formula 83. > > Both performed well, but the Formula 83 has won the test. It > performed most > like xylene and required little to no changes in my procedures. > > I did choose Richard Allan Mounting Media, as it worked great with the > Formula 83 and was suggested by the CBG rep. The mounting media I was > using was not compatible with the sub. > > Formula 83 did dry slides slightly faster as they came off the stain > line, > but the slide quality still performed to the boss man's liking. > > Thank you all for your input. Back to work I go. > > Rebecca > > > ------------------------------ > > Message: 14 > Date: Fri, 17 Aug 2007 16:08:53 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: "'Jackie M O'Connor'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <000801c7e0d8$24e36ab0$6412a8c0@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Embalming is no usual procedure in Austria. Perhaps that's the cause, > why > there wasn't any comment on this issue in the media. And the funeral > homes > are quiet institutions.... > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Jackie M > O'Connor > Gesendet: Freitag, 17. August 2007 15:20 > An: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Betreff: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 15 > Date: Fri, 17 Aug 2007 07:09:04 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Sensitivity and specificity > To: Laurie Reilly , > histonet@lists.utsouthwestern.edu > Message-ID: <269809.68833.qm@web61221.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Laurie: > Both Gudrun and Kemlo are right, let me just try to put it in other > way: > > 1-specificity: your detection system will react ONLY with the > targeted component (it is, after all, an antigen/antibody reaction > based on the quaternary protein structure of both), and > > 2-sensitivity: your detection system will detect ALL of the tageted > component, even in its lowest concentrations. The more sensitive your > detection system is, the less amount of the tageted compound it will > be able to detect. > > Ren? J. > > Laurie Reilly wrote: > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Choose the right car based on your needs. Check out Yahoo! Autos new > Car Finder tool. > > ------------------------------ > > Message: 16 > Date: Fri, 17 Aug 2007 07:11:11 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: Jackie M O'Connor , > histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: <88243.841.qm@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Not from Europe, but we just should follow their example! > Ren? J. > > Jackie M O'Connor wrote: Funny thing - I > found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Got a little couch potato? > Check out fun summer activities for kids. > > ------------------------------ > > Message: 17 > Date: Fri, 17 Aug 2007 15:25:41 +0100 > From: "kemlo" > Subject: RE: [Histonet] Sensitivity and specificity > To: "'Rene J Buesa'" , "'Laurie Reilly'" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > I said it more succinctly . > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: 17 August 2007 15:09 > To: Laurie Reilly; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Sensitivity and specificity > > Laurie: > Both Gudrun and Kemlo are right, let me just try to put it in other > way: > > 1-specificity: your detection system will react ONLY with the > targeted > component (it is, after all, an antigen/antibody reaction based on the > quaternary protein structure of both), and > > 2-sensitivity: your detection system will detect ALL of the tageted > component, even in its lowest concentrations. The more sensitive your > detection system is, the less amount of the tageted compound it will > be able > to detect. > > Ren? J. > > Laurie Reilly wrote: > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Choose the right car based on your needs. Check out Yahoo! Autos new > Car > Finder tool. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 18 > Date: Fri, 17 Aug 2007 15:27:29 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: "Jackie M O'Connor" , > , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Apparently the recent accession of Romania to the EEC, which of > course includes Transylvania has been the main stakeholders in > this legislation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > M > O'Connor > Sent: 17 August 2007 14:20 > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 19 > Date: Fri, 17 Aug 2007 09:47:46 -0500 > From: "Cheri Miller" > Subject: RE: [Histonet] prostate needle biopsies > To: "'pereirafamily'" , > > Message-ID: <002c01c7e0dd$94e37f80$3402a8c0@plab.local> > Content-Type: text/plain; charset="US-ASCII" > > We love the blue sponges. > > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If you are not the addressee intended / indicated or agent > responsible for delivering it to the addressee, you are hereby > notified that you are in possession of confidential and privileged > information. Any dissemination, distribution, or copying of this > e-mail is strictly prohibited. If you have received this message in > error, please notify the sender immediately and delete this email from > your system. > > > > > ------------------------------ > > Message: 20 > Date: Fri, 17 Aug 2007 10:01:18 -0500 > From: "Weston, Bernadette" > Subject: [Histonet] wikipedia > To: > Message-ID: > > <5213EA72AB13584EBD29CE54E1A66F0502954233@CPRTEVS02.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Not to cast doubt but are we sure Wikipedia has got this right? They > have been called questioned recently. > > Bernadette Weston HT > Histology Supervisor > Barberton Citizens Hospital > 330 615 3980 > bweston@barbhosp.com > > > > > ------------------------------ > > Message: 21 > Date: Fri, 17 Aug 2007 10:04:43 -0500 > From: "Bernice Frederick" > Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: "'Edwards, R.E.'" , "'Jackie M O'Connor'" > , , > > Message-ID: <000001c7e0df$f4ba3000$d00f7ca5@lurie.northwestern.edu> > Content-Type: text/plain; charset="us-ascii" > > You mean there is a clone of Joe in the U.K.? Love it!!!! > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Edwards, > R.E. > Sent: Friday, August 17, 2007 9:27 AM > To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Apparently the recent accession of Romania to the EEC, which of > course includes Transylvania has been the main stakeholders in > this legislation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > M > O'Connor > Sent: 17 August 2007 14:20 > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 22 > Date: Fri, 17 Aug 2007 11:09:54 -0500 > From: "Douglas D Deltour" > Subject: RE: {SPAM?} RE: [Histonet] prostate needle biopsies > To: "'Cheri Miller'" , "'pereirafamily'" > , > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Ask your processor if it loves the blue sponges. :) > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity > to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri > Miller > Sent: Friday, August 17, 2007 9:48 AM > To: 'pereirafamily'; histonet@lists.utsouthwestern.edu > Subject: {SPAM?} RE: [Histonet] prostate needle biopsies > > We love the blue sponges. > > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 23 > Date: Fri, 17 Aug 2007 10:12:39 -0500 > From: "Deathridge, Mary Ann" > Subject: [Histonet] job postings > To: > Message-ID: > <113162616B6440479A211712490222C684C4CA@mailbe10.mc.vanderbilt.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Vanderbilt University Medical Center, Nashville, TN. has (2) two > histotechnologist (ASCP) position openings in the histopathology lab. > We are looking for techs with immunohistochemistry experience, as well > as, general histology skills. > VUMC offers a great benefits package and a highly competitive salary > range in line with today's market. > Vanderbilt prides itself in being one of the best places to work with > high employee satisfaction. > Check out the web site at www.mc.vanderbilt.edu > go to HR, available jobs, to post > resume. > Feel free to contact me for further information. > > MaryAnn Deathridge, BS., HT (ASCP) > Supervisor, Histopathology > (615) 343-7012 > > > ------------------------------ > > Message: 24 > Date: Fri, 17 Aug 2007 11:26:11 -0400 > From: "Woodward, Denise" > Subject: [Histonet] B&B Gram negative color change > To: "Weston, Bernadette" , > > Message-ID: > <40AC6D73C2B95C4CA21B26B7BF380C401662FC@EXCHANGED.mgmt.ad.uconn.edu> > Content-Type: text/plain; charset="us-ascii" > > > Hello all, > > We recently noticed that our B&B positive controls change color over > time after staining. It happens in as little time as a week. > The Gram negative organisms change hue from a nice pink/red to a purple > color. The Gram positive organisms remain blue. > Anyone have any clues as to why/how this is happening? > Sorry to ask a difficult question on a Friday but this one has been > bothering me for a few days. > Thanks, > Denise Long Woodward > UConn > Dept. of Pathobiology and Veterinary Sciences > Storrs, CT > > > > ------------------------------ > > Message: 25 > Date: Fri, 17 Aug 2007 08:28:13 -0700 (PDT) > From: Paula Pierce > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: Histonet > Message-ID: <171741.71965.qm@web50111.mail.re2.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > So, the "undead" of Transylvania have a large enough lobby to get all > of Europe against embalming with formaldehyde? ;) > > > In all honesty though, beware the slippery slope of banning something > for one use. Our astronaut's safety has become secondary to using > inferior materials for environmental purposes. > > > Paula Pierce, HTL(ASCP)HT > > ------------------------------ > > Message: 26 > Date: Fri, 17 Aug 2007 08:36:50 -0700 (PDT) > From: GLORIA MUNOZ > Subject: [Histonet] microwave reproducibility > To: histonet@lists.utsouthwestern.edu > Message-ID: <975935.62550.qm@web80206.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi HistoNetters: > Just wondering if any of you have a procedure to share on > microwave reproducibility for a store-bought, non-medical microwave. > We had our CAP inspection recently and were asked to come up with a > method to test the microwave. The inspector recommended heating > distilled water for one minute at each of the three different power > levels and documenting the results. Does anyone know of another, > maybe better, way? > Thanks a lot, enjoy reading your entries, and hope you all have a > restful weekend...........gloria > > > ------------------------------ > > Message: 27 > Date: Fri, 17 Aug 2007 08:55:41 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] B&B Gram negative color change > To: "Woodward, Denise" , "Weston, > Bernadette" , histonet@lists.utsouthwestern.edu > Message-ID: <60752.31377.qm@web61217.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If you do not totally wash/remove/differentiate between steps, that > could happen. > Ren? J. > > "Woodward, Denise" wrote: > > Hello all, > > We recently noticed that our B&B positive controls change color over > time after staining. It happens in as little time as a week. > The Gram negative organisms change hue from a nice pink/red to a purple > color. The Gram positive organisms remain blue. > Anyone have any clues as to why/how this is happening? > Sorry to ask a difficult question on a Friday but this one has been > bothering me for a few days. > Thanks, > Denise Long Woodward > UConn > Dept. of Pathobiology and Veterinary Sciences > Storrs, CT > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Need a vacation? Get great deals to amazing places on Yahoo! Travel. > > ------------------------------ > > Message: 28 > Date: Fri, 17 Aug 2007 08:57:12 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: Paula Pierce , Histonet > > Message-ID: <839201.66477.qm@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Remember how the Transilvanian "undeads"increase in numer by neck > bitting/blood sucking. > Ren? J. > > Paula Pierce wrote: > So, the "undead" of Transylvania have a large enough lobby to get > all of Europe against embalming with formaldehyde? ;) > > > In all honesty though, beware the slippery slope of banning something > for one use. Our astronaut's safety has become secondary to using > inferior materials for environmental purposes. > > > Paula Pierce, HTL(ASCP)HT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Luggage? GPS? Comic books? > Check out fitting gifts for grads at Yahoo! Search. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 45, Issue 24 > **************************************** From jnocito <@t> satx.rr.com Fri Aug 17 11:51:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 17 11:51:42 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 References: Message-ID: <002701c7e0ee$d43638b0$d49eae18@yourxhtr8hvc4p> Joe who? ----- Original Message ----- From: "Kate Hilburn" To: "Histonet" Sent: Friday, August 17, 2007 11:10 AM Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 I would think the Transylvanian vampires would be far more concerned about AIDS than cancer. Kate Hilburn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, August 17, 2007 11:28 AM To: Histonet Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Fri Aug 17 11:57:49 2007 From: kemlo <@t> f2s.com (kemlo) Date: Fri Aug 17 11:58:36 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: References: <81196.87505.qm@web50301.mail.re2.yahoo.com> Message-ID: <5BC758EB0DCE4B6B9AD45376FB2D4C21@KemloPC> Ever seen an embalmed body autopsied? Very strange, you see how the fixative penetrates, or not. Saw an embalmed body 'cut up' very strange colour. How many cadavers get cancer after embalming? Am I missing something? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: 17 August 2007 15:27 To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Apparently the recent accession of Romania to the EEC, which of course includes Transylvania has been the main stakeholders in this legislation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 17 August 2007 14:20 To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcates <@t> synecor.com Fri Aug 17 12:37:52 2007 From: lcates <@t> synecor.com (Lynne Cates) Date: Fri Aug 17 12:38:05 2007 Subject: [Histonet] Safran du Gatinais Message-ID: Need large quantity of saffron. Does anyone have a contact they would be willing to share? Thanks Lynne Cates, HT (ASCP) Supervisor Histopathology Services SyneCor, LLC 3908 Patriot Drive Suite 170 Durham, NC 27703 Tel (919) 541-9977 X 119 Fax (919) 541-9975 From vazquezr <@t> ohsu.edu Fri Aug 17 12:42:48 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Aug 17 12:43:30 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Message-ID: Fungal Joe the Toe, I mean Jungle Joe the toe...Happy Frieday!!! (typo on purpose);>) Robyn >>> "Joe Nocito" 8/17/2007 9:51 AM >>> Joe who? ----- Original Message ----- From: "Kate Hilburn" To: "Histonet" Sent: Friday, August 17, 2007 11:10 AM Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 I would think the Transylvanian vampires would be far more concerned about AIDS than cancer. Kate Hilburn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, August 17, 2007 11:28 AM To: Histonet Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Aug 17 12:49:50 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Aug 17 12:50:14 2007 Subject: [Histonet] Polyethylene glycol? Message-ID: <8C9AF0E24EDDBBB-54C-4AC8@webmail-md17.sysops.aol.com> Tim Coskran somewhere asks about demonstrating polyethylene glycol in tissue samples. Does he mean polyethylene as in prosthetic implants?. Dr? Vinh in the Orthopedic Department at the AFIP co-authored?a paper published?in about 1990 entitled " Identification of polymeric debris is tissue sections by the?Oil Red O staining method."?? ? I am pretty sure polyethylene glycol is a fluid and would not survive paraffin processing. Michael Titford Pathology - USA Mobile AL USA ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From gu.lang <@t> gmx.at Fri Aug 17 12:56:28 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 17 12:56:43 2007 Subject: [Histonet] alcohol fixation image (2nd try) Message-ID: <000101c7e0f7$f0527c30$6412a8c0@dielangs.at> Hi, I have to admit, that I need help with basic histotechnical knowledge. Please, can someone describe me the "alcohol fixation image" of tissue, especially chromatin? And would poorly formalin-fixed tissue show less chromatin-structure or more intense chromatin-structure, after the usual VIP-processing. (50-70-96-96-100-100 alc.) due to the ethanol-effect? Thanks in advance Gudrun Lang From DEllenburg2 <@t> stfrancishealth.org Fri Aug 17 12:59:42 2007 From: DEllenburg2 <@t> stfrancishealth.org (Ellenburg, Deborah) Date: Fri Aug 17 13:00:01 2007 Subject: [Histonet] Thermo Shandon Excelsior Processor In-Reply-To: Message-ID: Jennifer, We installed an Excelsior in 2003. Give me a call and I will share our experience with you. Debbie Ellenburg, HT (ASCP) Histology Supervisor 864-255-1582 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, August 17, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 45, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: microwave reproducibility (Rene J Buesa) 2. RE: Ban on Formaldehyde in Europe deadline 22SEP07 (Kate Hilburn) 3. Thermo Shandon Excelsior tissue processor (Jennifer Springsteen) 4. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Joe Nocito) 5. RE: Ban on Formaldehyde in Europe deadline 22SEP07 (kemlo) ---------------------------------------------------------------------- Message: 1 Date: Fri, 17 Aug 2007 08:58:55 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] microwave reproducibility To: GLORIA MUNOZ , histonet@lists.utsouthwestern.edu Message-ID: <946594.66477.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Follow your inspector's suggestion. That is very good way "to prove" reproducibility. Ren? J. GLORIA MUNOZ wrote: Hi HistoNetters: Just wondering if any of you have a procedure to share on microwave reproducibility for a store-bought, non-medical microwave. We had our CAP inspection recently and were asked to come up with a method to test the microwave. The inspector recommended heating distilled water for one minute at each of the three different power levels and documenting the results. Does anyone know of another, maybe better, way? Thanks a lot, enjoy reading your entries, and hope you all have a restful weekend...........gloria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. ------------------------------ Message: 2 Date: Fri, 17 Aug 2007 12:10:38 -0400 From: "Kate Hilburn" Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" I would think the Transylvanian vampires would be far more concerned about AIDS than cancer. Kate Hilburn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, August 17, 2007 11:28 AM To: Histonet Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 17 Aug 2007 11:25:29 -0500 From: Jennifer Springsteen Subject: [Histonet] Thermo Shandon Excelsior tissue processor To: histonet@lists.utsouthwestern.edu Message-ID: <4f3f3ed37712d58e2434149799c80f38@umn.edu> Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed Does anyone use a Thermo Shandon Excelsior tissue processor? We just bought a new one, but found that the "state-of-the-art" molded polymer chamber has small cracks at some of the seams, which would make reagent containment and vacuum processing a bit difficult. Thermo is sending someone to assess/replace the unit, but I want to know if anyone else has experience with the machine to see if this is a common problem? Has anyone ever seen cracks develop after repeated use? Any idea whether the Shandon Pathcentre processor is better? Thanks! -Jennifer Springsteen, Lab Manager Lillehei Heart Institute Histopathology & Microscopy Core University of Minnesota School of Medicine Minneapolis, MN 55455 On Aug 17, 2007, at 11:10 AM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. detecting polyethylene glycol (Coskran, Timothy M) > 2. Question about CLIA requirements (jimr0712@comcast.net) > 3. Re: Question about CLIA requirements (Joe Nocito) > 4. prostate needle biopsies (pereirafamily) > 5. an easy solution RE: [Histonet] prostate needle biopsies > (Cheryl R. Kerry) > 6. Sensitivity and specificity (Laurie Reilly) > 7. AW: [Histonet] Sensitivity and specificity (Gudrun Lang) > 8. RE: Sensitivity and specificity (kemlo) > 9. RE: detecting polyethylene glycol (kemlo) > 10. Re: an easy solution RE: [Histonet] prostate needle biopsies > (Rene J Buesa) > 11. Chattanooga area.... (karenadams@comcast.net) > 12. Ban on Formaldehyde in Europe deadline 22SEP07 (Jackie M > O'Connor) > 13. Xylene Substitute Testing Results (Rebecca Jones) > 14. AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > (Gudrun Lang) > 15. Re: Sensitivity and specificity (Rene J Buesa) > 16. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Rene J Buesa) > 17. RE: Sensitivity and specificity (kemlo) > 18. RE: Ban on Formaldehyde in Europe deadline 22SEP07 (Edwards, > R.E.) > 19. RE: prostate needle biopsies (Cheri Miller) > 20. wikipedia (Weston, Bernadette) > 21. RE: Ban on Formaldehyde in Europe deadline 22SEP07 > (Bernice Frederick) > 22. RE: {SPAM?} RE: [Histonet] prostate needle biopsies > (Douglas D Deltour) > 23. job postings (Deathridge, Mary Ann) > 24. B&B Gram negative color change (Woodward, Denise) > 25. Ban on Formaldehyde in Europe deadline 22SEP07 (Paula Pierce) > 26. microwave reproducibility (GLORIA MUNOZ) > 27. Re: B&B Gram negative color change (Rene J Buesa) > 28. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 16 Aug 2007 15:42:12 -0400 > From: "Coskran, Timothy M" > Subject: [Histonet] detecting polyethylene glycol > To: > Message-ID: > <3F4063B77CC1F445933078B93A0A0C720311D4D4@groamrexm05.amer.pfizer.com> > Content-Type: text/plain; charset="US-ASCII" > > Does anyone know of a histochemical or IHC method for detecting > polyethylene glycol in tissue samples? > > > > Thanks, > > Tim Coskran > > DSRD-Groton > > ---------------------------------------------------------------------- > LEGAL NOTICE > Unless expressly stated otherwise, this message is confidential and > may be privileged. It is intended for the addressee(s) only. Access > to this E-mail by anyone else is unauthorized. If you are not an > addressee, any disclosure or copying of the contents of this E-mail or > any action taken (or not taken) in reliance on it is unauthorized and > may be unlawful. If you are not an addressee, please inform the > sender immediately. > > ------------------------------ > > Message: 2 > Date: Thu, 16 Aug 2007 21:39:54 +0000 > From: jimr0712@comcast.net > Subject: [Histonet] Question about CLIA requirements > To: histonet@lists.utsouthwestern.edu (Histonet2), > histonet-request@lists.utsouthwestern.edu (Histonet1), > histonet-request@lists.utsouthwestern.edu (Histonet) > Message-ID: > > <081620072139.27209.46C4C42A0007B15200006A492216554886CDCEC9CF9D030706@ > comcast.net> > > > The IT staff at a hospital that we service, stated that CLIA requires > the computer hardware to be under a CLIA license. Is this true ? Have > not heard this before. > > Thanks. > > -- > Jim Robinson, M.S., HTL/HT (ASCP) > Director of Laboratory Operations > Orizon Diagnostics, LLC > 102 Chestnut Avenue > Westmont, Illinois 60559 > jrobinson@orizondiagnostics.com > 630-321-1506 (fax) > 630-230-6325 > > > > ------------------------------ > > Message: 3 > Date: Thu, 16 Aug 2007 17:30:12 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Question about CLIA requirements > To: , "Histonet2" > , "Histonet1" > > Message-ID: <000b01c7e055$03717e40$d49eae18@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="Windows-1252"; > reply-type=original > > This is news to me and I just had a CLIA inspection last month. > > Joe > ----- Original Message ----- > From: > To: "Histonet2" ; "Histonet1" > ; "Histonet" > > Sent: Thursday, August 16, 2007 4:39 PM > Subject: [Histonet] Question about CLIA requirements > > >> The IT staff at a hospital that we service, stated that CLIA requires >> the >> computer hardware to be under a CLIA license. Is this true ? Have not >> heard this before. >> >> Thanks. >> >> -- >> Jim Robinson, M.S., HTL/HT (ASCP) >> Director of Laboratory Operations >> Orizon Diagnostics, LLC >> 102 Chestnut Avenue >> Westmont, Illinois 60559 >> jrobinson@orizondiagnostics.com >> 630-321-1506 (fax) >> 630-230-6325 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Thu, 16 Aug 2007 16:05:42 -0700 > From: "pereirafamily" > Subject: [Histonet] prostate needle biopsies > To: > Message-ID: <000601c7e059$f8a40b40$09650744@pereirafarm> > Content-Type: text/plain; charset="iso-8859-1" > > Is anyone using anything other than sponges in their cassettes for > prostate needle biopsies? If so, do you find that it works better? > > ------------------------------ > > Message: 5 > Date: Thu, 16 Aug 2007 19:10:51 -0500 > From: "Cheryl R. Kerry" > Subject: an easy solution RE: [Histonet] prostate needle biopsies > To: "'pereirafamily'" , > > Message-ID: <006a01c7e063$12e992f0$6701a8c0@CHERYLSLAPTOP> > Content-Type: text/plain; charset="US-ASCII" > > You'll laugh--but this works GREAT. On top of the sponges a couple > of labs > I've worked at used white C-fold paper towels cut to size to lay > against the > biopsies. They process well, lift RIGHT OFF without sticking as long > as the > toweling was damp when the needle bxs were laid on them. No > trebeculation > from the sponges--and they were FLAT. > > It would layer like this: > > cassette, > damp sponge, > cut paper towel, > biopsy(ies), > cut paper towel, > damp sponge, > cassette lid. > > If you wanted to dye them squirt eosin or merchurichrome on the > cassette > once closed and they stain. > > Amazing-and pretty much free. It works with EMB and other 'sticky' > stuff > like FNA buttons. > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one great tech at a time. > 800.756.3309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Fri, 17 Aug 2007 17:05:49 +1000 > From: Laurie Reilly > Subject: [Histonet] Sensitivity and specificity > To: > Message-ID: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au> > Content-Type: text/plain; charset="us-ascii" > > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > > > ------------------------------ > > Message: 7 > Date: Fri, 17 Aug 2007 10:02:25 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Sensitivity and specificity > To: "'Laurie Reilly'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <000901c7e0a4$f360e360$6412a8c0@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Sensitivity refers to the minimal ammount of antigen, that can be > detected > by the whole detection-system (primary antibody qualitiy/quantity; > secondary > antibody; activity of the used enzyme; etc.) > The sensitivity can be encreased by higher affine antibodies, more > "steps", > amplification. > > The specifity of a test refers to the fact, that the test is only > positiv, > if the tested substance is really present; the test must not be > positiv with > any other substance. For example, if an antibody crossreacts with > epitops on > various celltypes, the antibody and the test are not specific for a > unique > cell-type. - but the antibody itself is specific to its epitop. > > Hope this helps > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Laurie > Reilly > Gesendet: Freitag, 17. August 2007 09:06 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Sensitivity and specificity > > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 8 > Date: Fri, 17 Aug 2007 11:21:27 +0100 > From: "kemlo" > Subject: RE: [Histonet] Sensitivity and specificity > To: "'Laurie Reilly'" , > > Message-ID: <9453D8E8B8814677AA84C02BD8049AD9@KemloPC> > Content-Type: text/plain; charset="us-ascii" > > Doesn't sensitivity mean that it will stain all of 'it', even that > which > isn't 'it'. Specificity means that it only stains 'it' but sometimes > not all > of 'it'. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: 17 August 2007 08:06 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sensitivity and specificity > > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 9 > Date: Fri, 17 Aug 2007 11:25:09 +0100 > From: "kemlo" > Subject: RE: [Histonet] detecting polyethylene glycol > To: "'Coskran, Timothy M'" , > > Message-ID: <7F60092C0C774263B3EA2CD1855BFEC5@KemloPC> > Content-Type: text/plain; charset="us-ascii" > > Won't polyethylene glycol wash out? You won't be able to process it so > I > guess it's frozen sections, but they won't freeze if there's > polyethylene > glycol present will they? > > Unless it gets broken down into a metabolite then I'm at a loss. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Coskran, > Timothy M > Sent: 16 August 2007 20:42 > To: Histonet@pathology.swmed.edu > Subject: [Histonet] detecting polyethylene glycol > > Does anyone know of a histochemical or IHC method for detecting > polyethylene glycol in tissue samples? > > > > Thanks, > > Tim Coskran > > DSRD-Groton > > ---------------------------------------------------------------------- > LEGAL NOTICE > Unless expressly stated otherwise, this message is confidential and > may be > privileged. It is intended for the addressee(s) only. Access to this > E-mail by anyone else is unauthorized. If you are not an addressee, > any > disclosure or copying of the contents of this E-mail or any action > taken (or > not taken) in reliance on it is unauthorized and may be unlawful. If > you > are not an addressee, please inform the sender immediately. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 10 > Date: Fri, 17 Aug 2007 06:10:51 -0700 (PDT) > From: Rene J Buesa > Subject: Re: an easy solution RE: [Histonet] prostate needle biopsies > To: "Cheryl R. Kerry" , 'pereirafamily' > , histonet@lists.utsouthwestern.edu > Message-ID: <104128.57928.qm@web61215.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > You even could do WITHOUT the sponges, just the paper towel. > Ren? J. > > "Cheryl R. Kerry" wrote: > You'll laugh--but this works GREAT. On top of the sponges a couple > of labs > I've worked at used white C-fold paper towels cut to size to lay > against the > biopsies. They process well, lift RIGHT OFF without sticking as long > as the > toweling was damp when the needle bxs were laid on them. No > trebeculation > from the sponges--and they were FLAT. > > It would layer like this: > > cassette, > damp sponge, > cut paper towel, > biopsy(ies), > cut paper towel, > damp sponge, > cassette lid. > > If you wanted to dye them squirt eosin or merchurichrome on the > cassette > once closed and they stain. > > Amazing-and pretty much free. It works with EMB and other 'sticky' > stuff > like FNA buttons. > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one great tech at a time. > 800.756.3309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Park yourself in front of a world of choices in alternative vehicles. > Visit the Yahoo! Auto Green Center. > > ------------------------------ > > Message: 11 > Date: Fri, 17 Aug 2007 13:11:47 +0000 > From: karenadams@comcast.net > Subject: [Histonet] Chattanooga area.... > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <081720071311.28975.46C59E93000B65950000712F22070215539C030E0B0E020A9D0 > E05@comcast.net> > > Content-Type: text/plain > > Hello histonetters.....if anyone is on the server that works in or > near the Chattanooga area could you please write me back.....Thank > you! > > -- > Karen Adams > Pathology Laboratories West > 9303 Park West Blvd > Knoxville, TN 37923 > (865) 690-2111 FAX (865) 691-1623 > > ------------------------------ > > Message: 12 > Date: Fri, 17 Aug 2007 08:19:55 -0500 > From: "Jackie M O'Connor" > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > > ------------------------------ > > Message: 13 > Date: Fri, 17 Aug 2007 08:57:58 -0500 > From: "Rebecca Jones" > Subject: [Histonet] Xylene Substitute Testing Results > To: histonet > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all! > > Earlier this summer I asked many of you your opinion on a good xylene > substitute to use. I got great suggestions and tips from all of you. > I > decided to test two, Clear Rite and Formula 83. > > Both performed well, but the Formula 83 has won the test. It > performed most > like xylene and required little to no changes in my procedures. > > I did choose Richard Allan Mounting Media, as it worked great with the > Formula 83 and was suggested by the CBG rep. The mounting media I was > using was not compatible with the sub. > > Formula 83 did dry slides slightly faster as they came off the stain > line, > but the slide quality still performed to the boss man's liking. > > Thank you all for your input. Back to work I go. > > Rebecca > > > ------------------------------ > > Message: 14 > Date: Fri, 17 Aug 2007 16:08:53 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: "'Jackie M O'Connor'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <000801c7e0d8$24e36ab0$6412a8c0@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Embalming is no usual procedure in Austria. Perhaps that's the cause, > why > there wasn't any comment on this issue in the media. And the funeral > homes > are quiet institutions.... > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Jackie M > O'Connor > Gesendet: Freitag, 17. August 2007 15:20 > An: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Betreff: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 15 > Date: Fri, 17 Aug 2007 07:09:04 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Sensitivity and specificity > To: Laurie Reilly , > histonet@lists.utsouthwestern.edu > Message-ID: <269809.68833.qm@web61221.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Laurie: > Both Gudrun and Kemlo are right, let me just try to put it in other > way: > > 1-specificity: your detection system will react ONLY with the > targeted component (it is, after all, an antigen/antibody reaction > based on the quaternary protein structure of both), and > > 2-sensitivity: your detection system will detect ALL of the tageted > component, even in its lowest concentrations. The more sensitive your > detection system is, the less amount of the tageted compound it will > be able to detect. > > Ren? J. > > Laurie Reilly wrote: > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Choose the right car based on your needs. Check out Yahoo! Autos new > Car Finder tool. > > ------------------------------ > > Message: 16 > Date: Fri, 17 Aug 2007 07:11:11 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: Jackie M O'Connor , > histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: <88243.841.qm@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Not from Europe, but we just should follow their example! > Ren? J. > > Jackie M O'Connor wrote: Funny thing - I > found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Got a little couch potato? > Check out fun summer activities for kids. > > ------------------------------ > > Message: 17 > Date: Fri, 17 Aug 2007 15:25:41 +0100 > From: "kemlo" > Subject: RE: [Histonet] Sensitivity and specificity > To: "'Rene J Buesa'" , "'Laurie Reilly'" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > I said it more succinctly . > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: 17 August 2007 15:09 > To: Laurie Reilly; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Sensitivity and specificity > > Laurie: > Both Gudrun and Kemlo are right, let me just try to put it in other > way: > > 1-specificity: your detection system will react ONLY with the > targeted > component (it is, after all, an antigen/antibody reaction based on the > quaternary protein structure of both), and > > 2-sensitivity: your detection system will detect ALL of the tageted > component, even in its lowest concentrations. The more sensitive your > detection system is, the less amount of the tageted compound it will > be able > to detect. > > Ren? J. > > Laurie Reilly wrote: > Histonetters, > > > > Can anyone help with a concise definition of "sensitivity" and > "specificity" > as it relates to immunohistochemical reactions. > > > > Thanks and regards, Laurie. > > > > Mr. Laurie Reilly > > School of Veterinary & Biomedical Sciences > > James Cook University > > Townsville > > Queensland 4811 > > Australia > > > > Phone 07 4781 4468 > > Fax 07 4779 1526 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Choose the right car based on your needs. Check out Yahoo! Autos new > Car > Finder tool. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 18 > Date: Fri, 17 Aug 2007 15:27:29 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: "Jackie M O'Connor" , > , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Apparently the recent accession of Romania to the EEC, which of > course includes Transylvania has been the main stakeholders in > this legislation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > M > O'Connor > Sent: 17 August 2007 14:20 > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 19 > Date: Fri, 17 Aug 2007 09:47:46 -0500 > From: "Cheri Miller" > Subject: RE: [Histonet] prostate needle biopsies > To: "'pereirafamily'" , > > Message-ID: <002c01c7e0dd$94e37f80$3402a8c0@plab.local> > Content-Type: text/plain; charset="US-ASCII" > > We love the blue sponges. > > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If you are not the addressee intended / indicated or agent > responsible for delivering it to the addressee, you are hereby > notified that you are in possession of confidential and privileged > information. Any dissemination, distribution, or copying of this > e-mail is strictly prohibited. If you have received this message in > error, please notify the sender immediately and delete this email from > your system. > > > > > ------------------------------ > > Message: 20 > Date: Fri, 17 Aug 2007 10:01:18 -0500 > From: "Weston, Bernadette" > Subject: [Histonet] wikipedia > To: > Message-ID: > > <5213EA72AB13584EBD29CE54E1A66F0502954233@CPRTEVS02.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Not to cast doubt but are we sure Wikipedia has got this right? They > have been called questioned recently. > > Bernadette Weston HT > Histology Supervisor > Barberton Citizens Hospital > 330 615 3980 > bweston@barbhosp.com > > > > > ------------------------------ > > Message: 21 > Date: Fri, 17 Aug 2007 10:04:43 -0500 > From: "Bernice Frederick" > Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: "'Edwards, R.E.'" , "'Jackie M O'Connor'" > , , > > Message-ID: <000001c7e0df$f4ba3000$d00f7ca5@lurie.northwestern.edu> > Content-Type: text/plain; charset="us-ascii" > > You mean there is a clone of Joe in the U.K.? Love it!!!! > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Edwards, > R.E. > Sent: Friday, August 17, 2007 9:27 AM > To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Apparently the recent accession of Romania to the EEC, which of > course includes Transylvania has been the main stakeholders in > this legislation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > M > O'Connor > Sent: 17 August 2007 14:20 > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > > Funny thing - I found this information in Wikipedia.com and the related > link below. After 22SEPT2007 it will be illegal to use formaldehyde > as > embalming fluid in Europe because of it's carcinogenic properties. > Curious. Anyone in Europe have a comment? > Jackie O' > > > http://www.webwire.com/ViewPressRel.asp?aId=41468 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 22 > Date: Fri, 17 Aug 2007 11:09:54 -0500 > From: "Douglas D Deltour" > Subject: RE: {SPAM?} RE: [Histonet] prostate needle biopsies > To: "'Cheri Miller'" , "'pereirafamily'" > , > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Ask your processor if it loves the blue sponges. :) > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity > to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri > Miller > Sent: Friday, August 17, 2007 9:48 AM > To: 'pereirafamily'; histonet@lists.utsouthwestern.edu > Subject: {SPAM?} RE: [Histonet] prostate needle biopsies > > We love the blue sponges. > > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pereirafamily > Sent: Thursday, August 16, 2007 6:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate needle biopsies > > Is anyone using anything other than sponges in their cassettes for > prostate > needle biopsies? If so, do you find that it works better? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any > dissemination, > distribution, or copying of this e-mail is strictly prohibited. If > you have > received this message in error, please notify the sender immediately > and > delete this email from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 23 > Date: Fri, 17 Aug 2007 10:12:39 -0500 > From: "Deathridge, Mary Ann" > Subject: [Histonet] job postings > To: > Message-ID: > <113162616B6440479A211712490222C684C4CA@mailbe10.mc.vanderbilt.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Vanderbilt University Medical Center, Nashville, TN. has (2) two > histotechnologist (ASCP) position openings in the histopathology lab. > We are looking for techs with immunohistochemistry experience, as well > as, general histology skills. > VUMC offers a great benefits package and a highly competitive salary > range in line with today's market. > Vanderbilt prides itself in being one of the best places to work with > high employee satisfaction. > Check out the web site at www.mc.vanderbilt.edu > go to HR, available jobs, to post > resume. > Feel free to contact me for further information. > > MaryAnn Deathridge, BS., HT (ASCP) > Supervisor, Histopathology > (615) 343-7012 > > > ------------------------------ > > Message: 24 > Date: Fri, 17 Aug 2007 11:26:11 -0400 > From: "Woodward, Denise" > Subject: [Histonet] B&B Gram negative color change > To: "Weston, Bernadette" , > > Message-ID: > <40AC6D73C2B95C4CA21B26B7BF380C401662FC@EXCHANGED.mgmt.ad.uconn.edu> > Content-Type: text/plain; charset="us-ascii" > > > Hello all, > > We recently noticed that our B&B positive controls change color over > time after staining. It happens in as little time as a week. > The Gram negative organisms change hue from a nice pink/red to a purple > color. The Gram positive organisms remain blue. > Anyone have any clues as to why/how this is happening? > Sorry to ask a difficult question on a Friday but this one has been > bothering me for a few days. > Thanks, > Denise Long Woodward > UConn > Dept. of Pathobiology and Veterinary Sciences > Storrs, CT > > > > ------------------------------ > > Message: 25 > Date: Fri, 17 Aug 2007 08:28:13 -0700 (PDT) > From: Paula Pierce > Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: Histonet > Message-ID: <171741.71965.qm@web50111.mail.re2.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > So, the "undead" of Transylvania have a large enough lobby to get all > of Europe against embalming with formaldehyde? ;) > > > In all honesty though, beware the slippery slope of banning something > for one use. Our astronaut's safety has become secondary to using > inferior materials for environmental purposes. > > > Paula Pierce, HTL(ASCP)HT > > ------------------------------ > > Message: 26 > Date: Fri, 17 Aug 2007 08:36:50 -0700 (PDT) > From: GLORIA MUNOZ > Subject: [Histonet] microwave reproducibility > To: histonet@lists.utsouthwestern.edu > Message-ID: <975935.62550.qm@web80206.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi HistoNetters: > Just wondering if any of you have a procedure to share on > microwave reproducibility for a store-bought, non-medical microwave. > We had our CAP inspection recently and were asked to come up with a > method to test the microwave. The inspector recommended heating > distilled water for one minute at each of the three different power > levels and documenting the results. Does anyone know of another, > maybe better, way? > Thanks a lot, enjoy reading your entries, and hope you all have a > restful weekend...........gloria > > > ------------------------------ > > Message: 27 > Date: Fri, 17 Aug 2007 08:55:41 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] B&B Gram negative color change > To: "Woodward, Denise" , "Weston, > Bernadette" , histonet@lists.utsouthwestern.edu > Message-ID: <60752.31377.qm@web61217.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If you do not totally wash/remove/differentiate between steps, that > could happen. > Ren? J. > > "Woodward, Denise" wrote: > > Hello all, > > We recently noticed that our B&B positive controls change color over > time after staining. It happens in as little time as a week. > The Gram negative organisms change hue from a nice pink/red to a purple > color. The Gram positive organisms remain blue. > Anyone have any clues as to why/how this is happening? > Sorry to ask a difficult question on a Friday but this one has been > bothering me for a few days. > Thanks, > Denise Long Woodward > UConn > Dept. of Pathobiology and Veterinary Sciences > Storrs, CT > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Need a vacation? Get great deals to amazing places on Yahoo! Travel. > > ------------------------------ > > Message: 28 > Date: Fri, 17 Aug 2007 08:57:12 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 > To: Paula Pierce , Histonet > > Message-ID: <839201.66477.qm@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Remember how the Transilvanian "undeads"increase in numer by neck > bitting/blood sucking. > Ren? J. > > Paula Pierce wrote: > So, the "undead" of Transylvania have a large enough lobby to get > all of Europe against embalming with formaldehyde? ;) > > > In all honesty though, beware the slippery slope of banning something > for one use. Our astronaut's safety has become secondary to using > inferior materials for environmental purposes. > > > Paula Pierce, HTL(ASCP)HT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Luggage? GPS? Comic books? > Check out fitting gifts for grads at Yahoo! Search. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 45, Issue 24 > **************************************** ------------------------------ Message: 4 Date: Fri, 17 Aug 2007 11:51:15 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 To: "Kate Hilburn" , "Histonet" Message-ID: <002701c7e0ee$d43638b0$d49eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Joe who? ----- Original Message ----- From: "Kate Hilburn" To: "Histonet" Sent: Friday, August 17, 2007 11:10 AM Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 I would think the Transylvanian vampires would be far more concerned about AIDS than cancer. Kate Hilburn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, August 17, 2007 11:28 AM To: Histonet Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 17 Aug 2007 17:57:49 +0100 From: "kemlo" Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 To: "'Edwards, R.E.'" , "'Jackie M O'Connor'" , , Message-ID: <5BC758EB0DCE4B6B9AD45376FB2D4C21@KemloPC> Content-Type: text/plain; charset="US-ASCII" Ever seen an embalmed body autopsied? Very strange, you see how the fixative penetrates, or not. Saw an embalmed body 'cut up' very strange colour. How many cadavers get cancer after embalming? Am I missing something? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: 17 August 2007 15:27 To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Apparently the recent accession of Romania to the EEC, which of course includes Transylvania has been the main stakeholders in this legislation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 17 August 2007 14:20 To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 Funny thing - I found this information in Wikipedia.com and the related link below. After 22SEPT2007 it will be illegal to use formaldehyde as embalming fluid in Europe because of it's carcinogenic properties. Curious. Anyone in Europe have a comment? Jackie O' http://www.webwire.com/ViewPressRel.asp?aId=41468 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 45, Issue 25 **************************************** From jkiernan <@t> uwo.ca Fri Aug 17 13:12:18 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Aug 17 13:13:11 2007 Subject: [Histonet] Safran du Gatinais In-Reply-To: References: Message-ID: I typed "buy saffron" into Google, and it came up with 1,480,000 hits, including http://www.bulkfoods.com/saffron You can get an ounce for $62 John Kiernan Anatomy, UWO London, Canada. -- ----- Original Message ----- From: Lynne Cates Date: Friday, August 17, 2007 13:39 Subject: [Histonet] Safran du Gatinais To: histonet@lists.utsouthwestern.edu > Need large quantity of saffron. Does anyone have a contact they > would be > willing to share? > > Thanks > > > > Lynne Cates, HT (ASCP) > > Supervisor Histopathology Services > > SyneCor, LLC > > 3908 Patriot Drive > > Suite 170 > > Durham, NC 27703 > > Tel (919) 541-9977 X 119 > > Fax (919) 541-9975 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Fri Aug 17 13:46:19 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 17 13:46:56 2007 Subject: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <000101c7e0f7$f0527c30$6412a8c0@dielangs.at> References: <000101c7e0f7$f0527c30$6412a8c0@dielangs.at> Message-ID: <46C5ECFB.9050904@umdnj.edu> I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From bhewlett <@t> cogeco.ca Fri Aug 17 14:13:06 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Aug 17 14:14:22 2007 Subject: [Histonet] alcohol fixation image (2nd try) References: <000101c7e0f7$f0527c30$6412a8c0@dielangs.at> <46C5ECFB.9050904@umdnj.edu> Message-ID: <009001c7e102$a4c9c7e0$6500a8c0@mainbox> Geoff, What a memory!!! You are correct, the drawing was certainly present in pre-1960's Maximov and Bloom, we were tested on this as students. Bryan. ----- Original Message ----- From: "Geoff McAuliffe" To: Cc: Sent: Friday, August 17, 2007 2:46 PM Subject: Re: [Histonet] alcohol fixation image (2nd try) >I recall that an early edition (1960's or so) of one of the major histology >texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of >how and intestinal epithelial cell would look after various fixatives. Also >try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, >all of the major histo texts that are too long and encyclopedic for today's >medical student. > > Geoff > > > Gudrun Lang wrote: >> Hi, >> >> I have to admit, that I need help with basic histotechnical knowledge. >> Please, can someone describe me the "alcohol fixation image" of tissue, >> especially chromatin? >> And would poorly formalin-fixed tissue show less chromatin-structure or >> more >> intense chromatin-structure, after the usual VIP-processing. >> (50-70-96-96-100-100 alc.) due to the ethanol-effect? >> >> >> Thanks in advance >> >> Gudrun Lang >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew <@t> shaw.ca Fri Aug 17 15:00:20 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Aug 17 15:01:42 2007 Subject: [Histonet] Safran du Gatinais References: Message-ID: <000e01c7e109$3d9a0dd0$ff144246@yourlk4rlmsu> The most cost effective place to buy saffron is from either an East Indian food store or a Health Food store. It appears that most of the world's supply of saffron now originates in India, where it is still used quite a bit as a culinary spice. Even so, it is quite expensive. Do make sure you buy whole stigmata and not a powder. Bryan Llewellyn ----- Original Message ----- From: "Lynne Cates" To: Sent: Friday, August 17, 2007 10:37 AM Subject: [Histonet] Safran du Gatinais Need large quantity of saffron. Does anyone have a contact they would be willing to share? Thanks Lynne Cates, HT (ASCP) Supervisor Histopathology Services SyneCor, LLC 3908 Patriot Drive Suite 170 Durham, NC 27703 Tel (919) 541-9977 X 119 Fax (919) 541-9975 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 17 15:49:47 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 15:49:52 2007 Subject: [Histonet] Safran du Gatinais In-Reply-To: Message-ID: <992742.7078.qm@web61221.mail.yahoo.com> This safron from "bulkfoods" will be good for a "paella". Ren? J. John Kiernan wrote: I typed "buy saffron" into Google, and it came up with 1,480,000 hits, including http://www.bulkfoods.com/saffron You can get an ounce for $62 John Kiernan Anatomy, UWO London, Canada. -- ----- Original Message ----- From: Lynne Cates Date: Friday, August 17, 2007 13:39 Subject: [Histonet] Safran du Gatinais To: histonet@lists.utsouthwestern.edu > Need large quantity of saffron. Does anyone have a contact they > would be > willing to share? > > Thanks > > > > Lynne Cates, HT (ASCP) > > Supervisor Histopathology Services > > SyneCor, LLC > > 3908 Patriot Drive > > Suite 170 > > Durham, NC 27703 > > Tel (919) 541-9977 X 119 > > Fax (919) 541-9975 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From liz <@t> premierlab.com Fri Aug 17 16:21:40 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Aug 17 16:21:56 2007 Subject: [Histonet] Safran du Gatinais In-Reply-To: <07799F59B8F94CC0BBBC2A6880EADB49@PremierLab.local> References: <07799F59B8F94CC0BBBC2A6880EADB49@PremierLab.local> Message-ID: Make sure if you are buying the saffron from a bulk foods that you get spanish saffron there are saffrons that you can get from mexico which are very inexpensive. The quality is not the same for both food preparation and in staining this is based upon our experience, we had some mexican saffron that one of the techs bought at a food store and we tried it just to see if it would work, not good at all. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 17, 2007 3:16 PM To: John Kiernan; Lynne Cates Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safran du Gatinais This safron from "bulkfoods" will be good for a "paella". Ren? J. John Kiernan wrote: I typed "buy saffron" into Google, and it came up with 1,480,000 hits, including http://www.bulkfoods.com/saffron You can get an ounce for $62 John Kiernan Anatomy, UWO London, Canada. -- ----- Original Message ----- From: Lynne Cates Date: Friday, August 17, 2007 13:39 Subject: [Histonet] Safran du Gatinais To: histonet@lists.utsouthwestern.edu > Need large quantity of saffron. Does anyone have a contact they would > be willing to share? > > Thanks > > > > Lynne Cates, HT (ASCP) > > Supervisor Histopathology Services > > SyneCor, LLC > > 3908 Patriot Drive > > Suite 170 > > Durham, NC 27703 > > Tel (919) 541-9977 X 119 > > Fax (919) 541-9975 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/957 - Release Date: 8/16/2007 1:46 PM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/957 - Release Date: 8/16/2007 1:46 PM From vazquezr <@t> ohsu.edu Fri Aug 17 16:27:42 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Aug 17 16:28:03 2007 Subject: [Histonet] Safran du Gatinais Message-ID: I have seen it at Costco in the spice section, would this be the same of what you are talking about? It was $28-30 for a bottle of it. Robyn >>> "Bryan Llewellyn" 8/17/2007 1:00 PM >>> The most cost effective place to buy saffron is from either an East Indian food store or a Health Food store. It appears that most of the world's supply of saffron now originates in India, where it is still used quite a bit as a culinary spice. Even so, it is quite expensive. Do make sure you buy whole stigmata and not a powder. Bryan Llewellyn ----- Original Message ----- From: "Lynne Cates" To: Sent: Friday, August 17, 2007 10:37 AM Subject: [Histonet] Safran du Gatinais Need large quantity of saffron. Does anyone have a contact they would be willing to share? Thanks Lynne Cates, HT (ASCP) Supervisor Histopathology Services SyneCor, LLC 3908 Patriot Drive Suite 170 Durham, NC 27703 Tel (919) 541-9977 X 119 Fax (919) 541-9975 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Aug 17 16:38:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Aug 17 16:38:36 2007 Subject: [Histonet] Safran du Gatinais In-Reply-To: References: Message-ID: <6.0.0.22.1.20070817153732.01b3e830@gemini.msu.montana.edu> Try your local grocery store spice counter - cheapest source around and works just as well. At 11:37 AM 8/17/2007, you wrote: >Need large quantity of saffron. Does anyone have a contact they would be >willing to share? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From jcresor <@t> lcpath.com Fri Aug 17 17:57:51 2007 From: jcresor <@t> lcpath.com (Jennifer Cresor) Date: Fri Aug 17 17:57:58 2007 Subject: [Histonet] growing gram pos and neg Message-ID: <9b07f78c93a361469c69ae3b3c131e69@mail2.lcpath.com> Hello all, I would like to hear your ideas on the best way to grow gram positive and negative organisms in tissue. It would have to be tissue that is not fixed so, what do you use? Did you grow the organisms, inject into tissue and then put into incubator? or do you grow organisms on agar and place tissue on it and incubate? or in a broth? Thank you for your input. Jennifer Longview, Wa. jencres@lcpath.com From llewllew <@t> shaw.ca Fri Aug 17 18:18:05 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Aug 17 18:19:43 2007 Subject: [Histonet] Safran du Gatinais References: Message-ID: <002501c7e124$de130850$ff144246@yourlk4rlmsu> No, the stuff that comes in a bottle is grossly overpriced. The stuff from India comes in flat plastic containers about 5x2x1 cms, and is quite a bit cheaper than that. bryan Llewellyn ----- Original Message ----- From: Robyn Vazquez To: histonet@lists.utsouthwestern.edu ; llewllew@shaw.ca ; lcates@synecor.com Sent: Friday, August 17, 2007 2:27 PM Subject: Re: [Histonet] Safran du Gatinais I have seen it at Costco in the spice section, would this be the same of what you are talking about? It was $28-30 for a bottle of it. Robyn >>> "Bryan Llewellyn" 8/17/2007 1:00 PM >>> The most cost effective place to buy saffron is from either an East Indian food store or a Health Food store. It appears that most of the world's supply of saffron now originates in India, where it is still used quite a bit as a culinary spice. Even so, it is quite expensive. Do make sure you buy whole stigmata and not a powder. Bryan Llewellyn ----- Original Message ----- From: "Lynne Cates" To: Sent: Friday, August 17, 2007 10:37 AM Subject: [Histonet] Safran du Gatinais Need large quantity of saffron. Does anyone have a contact they would be willing to share? Thanks Lynne Cates, HT (ASCP) Supervisor Histopathology Services SyneCor, LLC 3908 Patriot Drive Suite 170 Durham, NC 27703 Tel (919) 541-9977 X 119 Fax (919) 541-9975 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Aug 17 22:30:03 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 17 22:30:07 2007 Subject: [Histonet] Formaldehyde Ban??? Message-ID: <582736990708172030r17fb0874t3886f23cb6a56f47@mail.gmail.com> Hi, Regarding the recent post saying that Europe is planning to eliminate formalin, I ask "How dumb is that?!" How can they completely wreck any correlation of testing between laboratories? Well remove the standard of course! Anyone that uses formalin would need to use something else. Has anyone thought of what they should use? One lab uses X while another uses Y. Exponentiate that for all the labs in Europe and eventually elsewhere. There will be absolutely no basis for comparison between facilities. With more molecular testing being used you'd think the tendency would be toward order rather than chaos. The only logical way to control this mess would be to make the law a replacement rather than a ban. Something like: All labs in Europe need to use 'X fixative'. Obviously 'X fixative' would need to be an open source formulation so no one company would have a monopoly on the market. (Ask Microsoft about how Europe feels about monopolies!) That would be a ruling I could agree with. This haphazard, willie-nillie, every lab for itself, switch just doesn't make any sense. By the way, I don't think Dracula would like the taste of any of these fixatives in his blood ... Blah! ... Bleagh!! Amos Brooks From gu.lang <@t> gmx.at Sat Aug 18 01:47:29 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Aug 18 01:47:36 2007 Subject: AW: [Histonet] Formaldehyde Ban??? In-Reply-To: <582736990708172030r17fb0874t3886f23cb6a56f47@mail.gmail.com> Message-ID: <002f01c7e163$a606e4b0$6412a8c0@dielangs.at> Amos, this ban (if it really exists) refers to embalming bodies in funeral ceremonies. - not to histology laboratories. - as far as I know; sure, I've been in vacancy for three weeks. Perhaps my whole histo-world has changed, when I come back. ;) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amos Brooks Gesendet: Samstag, 18. August 2007 05:30 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Formaldehyde Ban??? Hi, Regarding the recent post saying that Europe is planning to eliminate formalin, I ask "How dumb is that?!" How can they completely wreck any correlation of testing between laboratories? Well remove the standard of course! Anyone that uses formalin would need to use something else. Has anyone thought of what they should use? One lab uses X while another uses Y. Exponentiate that for all the labs in Europe and eventually elsewhere. There will be absolutely no basis for comparison between facilities. With more molecular testing being used you'd think the tendency would be toward order rather than chaos. The only logical way to control this mess would be to make the law a replacement rather than a ban. Something like: All labs in Europe need to use 'X fixative'. Obviously 'X fixative' would need to be an open source formulation so no one company would have a monopoly on the market. (Ask Microsoft about how Europe feels about monopolies!) That would be a ruling I could agree with. This haphazard, willie-nillie, every lab for itself, switch just doesn't make any sense. By the way, I don't think Dracula would like the taste of any of these fixatives in his blood ... Blah! ... Bleagh!! Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Aug 18 07:42:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 18 07:42:32 2007 Subject: [Histonet] growing gram pos and neg In-Reply-To: <9b07f78c93a361469c69ae3b3c131e69@mail2.lcpath.com> Message-ID: <83144.17207.qm@web61224.mail.yahoo.com> Jennifer: Why bother with such a complex approach? Just use appendix, with all its bacterial load as a (+) and (-) positive control. Ren? J. Jennifer Cresor wrote: Hello all, I would like to hear your ideas on the best way to grow gram positive and negative organisms in tissue. It would have to be tissue that is not fixed so, what do you use? Did you grow the organisms, inject into tissue and then put into incubator? or do you grow organisms on agar and place tissue on it and incubate? or in a broth? Thank you for your input. Jennifer Longview, Wa. jencres@lcpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From kemlo <@t> f2s.com Sat Aug 18 09:43:40 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sat Aug 18 09:44:27 2007 Subject: [Histonet] growing gram pos and neg In-Reply-To: <83144.17207.qm@web61224.mail.yahoo.com> References: <9b07f78c93a361469c69ae3b3c131e69@mail2.lcpath.com> <83144.17207.qm@web61224.mail.yahoo.com> Message-ID: <3B4EADCA4D8C49D78ED683B25C2E7D31@KemloPC> Unless it's infected with C-Diff; um is that + or - ve? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 18 August 2007 13:42 To: Jennifer Cresor; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] growing gram pos and neg Jennifer: Why bother with such a complex approach? Just use appendix, with all its bacterial load as a (+) and (-) positive control. Ren? J. Jennifer Cresor wrote: Hello all, I would like to hear your ideas on the best way to grow gram positive and negative organisms in tissue. It would have to be tissue that is not fixed so, what do you use? Did you grow the organisms, inject into tissue and then put into incubator? or do you grow organisms on agar and place tissue on it and incubate? or in a broth? Thank you for your input. Jennifer Longview, Wa. jencres@lcpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Aug 18 12:05:29 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Aug 18 12:05:42 2007 Subject: AW: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <46C5ECFB.9050904@umdnj.edu> Message-ID: <000c01c7e1b9$fb9eb4b0$0202fea9@dielangs.at> Geoff and Bryan, Unfortunatly I have no access to these books. But I hoped someone could describe in a few words the essential differences between formalin and formalin-fixed tissue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Freitag, 17. August 2007 20:46 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] alcohol fixation image (2nd try) I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From gu.lang <@t> gmx.at Sat Aug 18 12:14:25 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Aug 18 12:14:34 2007 Subject: AW: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <000c01c7e1b9$fb9eb4b0$0202fea9@dielangs.at> Message-ID: <000d01c7e1bb$3af076c0$0202fea9@dielangs.at> Uups, should be "formalin and alcohol-fixed". Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Samstag, 18. August 2007 19:05 An: 'Geoff McAuliffe' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] alcohol fixation image (2nd try) Geoff and Bryan, Unfortunatly I have no access to these books. But I hoped someone could describe in a few words the essential differences between formalin and formalin-fixed tissue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Freitag, 17. August 2007 20:46 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] alcohol fixation image (2nd try) I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sat Aug 18 12:25:00 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sat Aug 18 12:25:47 2007 Subject: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <000d01c7e1bb$3af076c0$0202fea9@dielangs.at> References: <000c01c7e1b9$fb9eb4b0$0202fea9@dielangs.at> <000d01c7e1bb$3af076c0$0202fea9@dielangs.at> Message-ID: <89C908213EE64882AC93D86C26CA3E65@KemloPC> Few words? Formalin takes longer to fix but is a 'soft' fixative; alcohol is a faster fixative but hardens and shrinks tissue if it's left in it too long. Formalin is an additive coagulant fixative (forms bonds between proteins) whilst alcohol is a non-additive coagulant fixative (does something with the tertiary structure of proteins). Tissue needs formalin, cells are OK with alcohol; Cytology fixatives tend to be alcoholic whilst Histology Fixatives aren't. A wide generalisation I know but words are sacred . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 18 August 2007 18:14 To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] alcohol fixation image (2nd try) Uups, should be "formalin and alcohol-fixed". Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Samstag, 18. August 2007 19:05 An: 'Geoff McAuliffe' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] alcohol fixation image (2nd try) Geoff and Bryan, Unfortunatly I have no access to these books. But I hoped someone could describe in a few words the essential differences between formalin and formalin-fixed tissue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Freitag, 17. August 2007 20:46 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] alcohol fixation image (2nd try) I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Sat Aug 18 12:28:37 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Sat Aug 18 12:28:41 2007 Subject: [Histonet] microwave reproducibility Message-ID: <893035.44803.qm@web58901.mail.re1.yahoo.com> Gloria, I agree with Rene, heating a known volume of water in the microwave for about 60 seconds and measuring the change in temperature of the water is probably the best way to document the reproducibility of the microwave. Just measure the temperature of the water before you heat it, then gently stir the water and measure the temperature again after you've heated it. Subtract the two and record the increase in temperature. You'll probably have to experiment with the volume of water you heat, since you'll want to heat the water to a point somewhere below its boiling point. It would probably be a good idea to make about 3 tests for each power level of the oven, then calculate an average of the 3 readings. Begin each test with water that's near room temperature. A good starting point would probably be to heat about 500 ml or 1 L of water for 60 seconds and see what you get. You can expect some variation in the results, probably a degree or two in temperature, but microwave ovens are remarkably reproducible in this respect. Heating a volume of water for a set time is the first step in calculating the output (in Watts) of your microwave, and there are formulas to take the results to the next step and actually calculate the wattage of the oven. The full calculations probably aren't necessary for day-to-day operation, though. With age, the magnetron in the oven (the part that emits the microwaves) can gradually become less efficient. In that case you would notice less of an increase in temperature of the water. You'll also have records to show whether the magnetron is working properly during normal operation. Factors like total available power, the condition of the high voltage transformer, the control circuits for the magnetron and lots of other things could affect the microwave's output, so it's a good idea to do this test on a "regular" basis. That probably means weekly or monthly, I don't know for sure. Maybe there are others on Histonet who have some suggestions here. Just be sure to always heat the same volume of water for the same length of time, and you'll have the records you need. Hope this helps. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments www.swpinet.com The Desert Southwest's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: GLORIA MUNOZ To: histonet@lists.utsouthwestern.edu Sent: Friday, August 17, 2007 8:36:50 AM Subject: [Histonet] microwave reproducibility Hi HistoNetters: Just wondering if any of you have a procedure to share on microwave reproducibility for a store-bought, non-medical microwave. We had our CAP inspection recently and were asked to come up with a method to test the microwave. The inspector recommended heating distilled water for one minute at each of the three different power levels and documenting the results. Does anyone know of another, maybe better, way? Thanks a lot, enjoy reading your entries, and hope you all have a restful weekend...........gloria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. http://autos.yahoo.com/carfinder/ From karin.daniels <@t> co.hennepin.mn.us Sat Aug 18 13:06:26 2007 From: karin.daniels <@t> co.hennepin.mn.us (karin.daniels@co.hennepin.mn.us) Date: Sat Aug 18 13:06:33 2007 Subject: [Histonet] Karin B. Daniels is out of the office. Message-ID: I will be out of the office starting Sat 08/18/2007 and will not return until Mon 08/27/2007. I will respond to your message when I return. From bhewlett <@t> cogeco.ca Sat Aug 18 16:09:23 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sat Aug 18 16:09:32 2007 Subject: [Histonet] alcohol fixation image (2nd try) References: <000c01c7e1b9$fb9eb4b0$0202fea9@dielangs.at> Message-ID: <003a01c7e1dc$0e4005c0$6500a8c0@mainbox> Gudrun, I can do better than that! I am sending you ( in a separate e-mail) photomicraphs of adjacent samples of human ileum optimally fixed in formaldehyde and alcohol. In addition I have also included the same material 'routinely' underfixed fixed in formaldehyde (8hours) then processed on a VIP. You can see the obvious differences in nuclear chromatin detail and shrinkage produced by alcohol fixation, the 'routinely' fixed material shows an intermediate morphology! In addition, there are differences in staining produced by the fixation. All sections were cut at the same thickness (4 micrometers) and stained by H&E in the same run. Hope this helps. Regards, Bryan ----- Original Message ----- From: "Gudrun Lang" To: "'Geoff McAuliffe'" Cc: Sent: Saturday, August 18, 2007 1:05 PM Subject: AW: [Histonet] alcohol fixation image (2nd try) Geoff and Bryan, Unfortunatly I have no access to these books. But I hoped someone could describe in a few words the essential differences between formalin and formalin-fixed tissue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Freitag, 17. August 2007 20:46 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] alcohol fixation image (2nd try) I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Sat Aug 18 19:53:26 2007 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Sat Aug 18 19:53:54 2007 Subject: [Histonet] Formaldehyde ban in Europe Message-ID: <46C79486.3040808@shaw.ca> Several European countries have already banned the use of formaldehyde as an embalming fluid; some other countries are considering a ban. There has been no suggestion that formaldehyde be banned as a fixative for histological purposes. So, we as a group of concerned users, have no need to go out searching for alternate fixatives. Doing a Google search for "formaldehyde ban Europe embalming" produced several hundred hits from a wide variety of sources (as most Google searches do). Some were relevant, some were from ecological groups wanting to ban formaldehyde along with almost everything else, and others were from people whose brains had obviously already been pickled in some other reagent. From the relevant ones, it seems that the embalmers have a history of being less than careful with their formaldehyde-containing fluids, consequently some of their group have developed formaldehyde-associated disorders. These are all conditions that we know about and use appropriate protocols to avoid. Again there was no suggestion that formaldehyde be banned as a tissue fixative. Logically, a total ban on formaldehyde would be impossible. Contrary to common beliefs, the amount of formaldehyde used for tissue fixation is a tiny fraction of the total amount produced each year. Formaldehyde, in huge quantities, is used in the manufacturing of many household and commercial products. The majority of formaldehyde is used in the production of polymers and other chemicals. When combined with phenol, urea, or melamine, formaldehyde produces a hard resin such as those used in plywood or carpeting. It is used as the wet-strength resin added to sanitary paper products such as facial tissue, table napkins, and roll towels. They are also foamed to make home insulation, or cast into molded products. Production of formaldehyde resins accounts for more than half of formaldehyde consumption. Formaldehyde is used to produce glues used in the manufacture of particleboard, plywood, veneers, and other wood products as well as spray-on insulating foams. The textile industry uses formaldehyde-based resins to make fabrics crease-resistant. Whether the enviromentalists like it or not, formaldehyde is unlikely to disappear anytime in the foreseeable future. But, we do need to handle it safely and be aware of its dangers. (It was cool and rainy afternoon here in Kamloops, so no gardening, no golf, just a Google search and a few e-mails). Paul Bradbury, Kamloops, BC Canada ^ From jkiernan <@t> uwo.ca Sat Aug 18 23:51:59 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Aug 18 23:52:05 2007 Subject: [Histonet] Formaldehyde Ban - - at funerals ? almost funny. Message-ID: ---Original Message--- > This ban (if it really exists) refers to embalming bodies > in funeral ceremonies. ... Like this? To the sound of mournful music the lead-lined oaken coffin was slowly carried up the aisle and placed on a plinth before the altar. The undertaker, using a stainless steel brace and bit that shone like burnished silver, drilled a hole in the lid and inserted a large plastic funnel. His burly assistant slowly and reverently poured in fifty-four litres of environmentally friendly embalming fluid, warranted free of formaldehyde, while the choir sang six verses of "For all the saints," obscuring the gurglings from the funnel. The undertaker then took a cork, carried to him on a satin cushion by one of the youngest relatives of the deceased. He elegantly plugged the hole in the coffin's lid while his assistant inconspicuously carried away the funnel and the six dozen-size cardboard boxes of empty vodka bottles. After several long speeches and a brief prayer for the soul of the deceased, the coffin containing the newly embalmed body was taken to the crematorium. This deprived the cemetery worms of a free feast and booze-up, but they had no way of knowing that. (Apologies to Sylvie Krin for copying his style but not very well. That's for Brit histonetters and expats who read Private Eye. ) John Kiernan (Both Brit and Canadian) London, Ontario --- From Maxim_71 <@t> mail.ru Sun Aug 19 00:09:06 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sun Aug 19 00:18:12 2007 Subject: [Histonet] Specimens after radiation treatment Message-ID: <1104014483.20070819090906@mail.ru> Dear Histonetters: Our department now and then receive surgical specimens after radiation/chemotherapy treatment. Tissues came from breast and skin. These specimens very poor fixes, processes and cut. Posfixation in 10% NBF, Davidson, Bouin and reprocessing (by Johnson method) do not help. There is any way to improve their quality? We deal with FFPE secions for diagnostic purposes. Any suggestions would be appreciated. Thank you, Peshkov Maxim, Russia, Taganrog. From Rcartun <@t> harthosp.org Sun Aug 19 10:45:04 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Aug 19 10:45:27 2007 Subject: [Histonet] HBV+ control tissue Message-ID: <46C82D4002000077000079A3@gwmail.harthosp.org> I just saw the most incredibly positive hepatitis B surface antigen immunoperoxidase stain that I have ever seen! It looks like we have the entire liver (the patient was transplanted). I realize that most labs don't do IHC for HBV, but if you are looking for control tissue please contact me and I'll see what I can do. You must include your name, complete mailing address, telephone number, and an express mail (FedEx, UPS, etc.) account number. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From rsrichmond <@t> aol.com Sun Aug 19 14:26:24 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Sun Aug 19 14:26:34 2007 Subject: [Histonet] Re: Formaldehyde ban in Europe In-Reply-To: <200708191300.a6e46c8772228@rly-da05.mx.aol.com> References: <200708191300.a6e46c8772228@rly-da05.mx.aol.com> Message-ID: <8C9B0ADF7196E35-8C8-7D40@FWM-D19.sysops.aol.com> Embalming of human bodies has been widely practiced in the USA ever since the Civil War (1861-1865), but has come into widespread use in Europe much more recently. Almost all bodies in the USA are embalmed before burial in a sealed coffin which in turn is put in a concrete vault. (I shudder to think what will become of these in the next, say, million years.) Bodies are also routinely embalmed before cremation. Embalming was originally done with potassium arsenite, said to be the best of all embalming fluids, which was banned many years ago not for environmental reasons, but because it interfered with postmortem toxicologic investigation in suspected poisoning. Most present-day embalming fluids contain formaldehyde, sometimes with ethanol and phenol, and a red dye that makes the body look more lifelike. Several gallons of embalming fluid are pumped through the arterial system through multiple arteriotomies in the carotid, axillary, and femoral arteries. Arterial embalming can be done before or after autopsy. Done before autopsy, it gives the viscera and muscles an odd red color, but doesn't change texture a great deal. It's really quite a convenient way to do an autopsy - I've done many that way - but it of course precludes bacterial cultures. The longer the body will lie around before being buried, the higher the concentration of formaldehyde used - the trade-off is that a more heavily embalmed body is less pliable, so that the embalmer cannot so easily make the face look lifelike. Embalmed bodies are then displayed in funeral homes and in participating churches (not in MY church, thank you - I'm an Episcopalian), laid out in expensive caskets (never say coffins). Formaldehyde-free embalming fluids are used with jaundiced bodies, which if embalmed with formaldehyde turn a bright green color (from biliverdin) singularly resistant to covering up with make-up. Halacha (Jewish law) prohibits embalming as well as public display of dead bodies, and I think Islamic law does also. I don't know of any Christian denomination that prohibits it. No Histonetters on Mars are going to believe any of this. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rsrichmond <@t> aol.com Sun Aug 19 15:40:13 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Sun Aug 19 15:40:22 2007 Subject: [Histonet] Re: Safran du Gatinais In-Reply-To: <200708181300.19446c725a82d2@rly-yb04.mx.aol.com> References: <200708181300.19446c725a82d2@rly-yb04.mx.aol.com> Message-ID: <8C9B0B84708AD06-8C8-7ECE@FWM-D19.sysops.aol.com> Lynne Cates in Durham NC asks about buying saffron in bulk for histologic use. Saffron, a culinary spice and coloring agent most familiarly used in paella, consists of the stigmas of the flowers of Crocus sativus. The spice has a very strong odor, and also contains a dye of some histologic interest. Because of its labor-intensive production (do YOU want to spend your days picking the sex organs out of itty bitty flowers?) saffron's extremely expensive. Saffron is used histologically as a connective tissue stain, traditionally in one of the many techniques attributed to Dr. Masson, and in the Movat pentachrome stain. It dyes collagen a yellow-orange color that contrasts subtly with eosin. Saffron has a Colour Index number (75100) and is described in the 9th edition (I don't have the 10th) of Conn's Biological stains. The active coloring matter is called crocin, composed of crocetin and gentobiose. To prepare the stain, the dye is extracted from the crude spice with ethanol. Because saffron is so expensive, the WHO tumor fascicles (in the 1960's) suggested extracting the dye into ethanol using a reflux condenser to achieve maximum yield. This alcohol extract has an obnoxious medicinal smell. A look-alike, safflower (Carthamus tinctorius), sometimes called dyer's saffron or bastard saffron, is odorless and contains a different dye, carthamin or carthamone, chemically unrelated. I have never seen safflower referred to as a histologic stain. Safflower is sometimes referred to as saffron, and I'd be careful not to buy it for histologic use - remember it's odorless. (Safflower is grown commercially as an oil seed.) Saffron was historically grown in France and Spain. It is still grown commercially in Spain, but most of it is grown in India. The traditional histologic designation "safran du G?tinais" referred to the French product, which I think is no longer available. (Saffron was grown in England centuries ago, hence the place name Saffron Walden.) I checked a high-end spice dealer, Penzeys Spices (disclaimer - my wife orders a box of spices from them about once a month), and found saffron for US$10 to 15 a gram, retailed in gram quantities, depending on the source. I suspect it could be ordered from India, perhaps through an Indian grocery store, for less, but I'd want to be awfully careful I was getting Crocus sativus. Apparently lower grades of saffron can be bought in bulk for a dollar or two a gram. The Wikipedia article on saffron is worth reading. According to Wikipedia the coat of arms of Saffron Walden is "Vert within a representation of town walls having two towers and a Gateway between towers Argent three Saffron Flowers issuant from the battlements of the gateway blown and showing the stamens proper And for the Crest On a Wealth of the Colours Upon a Chapeau Gules turned up Ermine a Lion rampant Azure grasping in the dexter paw a representation of the Ancient Mace of the Borough of Saffron Walden proper." Bob Richmond Samurai Pathologist, histoantiquarian and occasional blazoner wannabe Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From pruegg <@t> ihctech.net Sun Aug 19 16:05:38 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Aug 19 16:02:34 2007 Subject: [Histonet] Re: Safran du Gatinais In-Reply-To: <8C9B0B84708AD06-8C8-7ECE@FWM-D19.sysops.aol.com> Message-ID: <200708192102.l7JL2OCq065813@pro12.abac.com> Being a gardener myself, I was wondering why we don't just grow some of our own Crocus plants, or is the common Crocus plant not the same plant that produces the Saffron from India these days, or perhaps you would have to grow so much of it to yield enough of the stigmas?????? I know Crocus grows in Colorado, even higher than I, I once saw a huge yard full of Crocus plants in Aspen, CO at the end of the summer, of course it would die off during the winter. Is my 5 acres in Colorado big enough, maybe I can grow Crocus as my cash crop? When I lived in California I had a house full of Crocus plants, they are very common there. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Sunday, August 19, 2007 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Safran du Gatinais Lynne Cates in Durham NC asks about buying saffron in bulk for histologic use. Saffron, a culinary spice and coloring agent most familiarly used in paella, consists of the stigmas of the flowers of Crocus sativus. The spice has a very strong odor, and also contains a dye of some histologic interest. Because of its labor-intensive production (do YOU want to spend your days picking the sex organs out of itty bitty flowers?) saffron's extremely expensive. Saffron is used histologically as a connective tissue stain, traditionally in one of the many techniques attributed to Dr. Masson, and in the Movat pentachrome stain. It dyes collagen a yellow-orange color that contrasts subtly with eosin. Saffron has a Colour Index number (75100) and is described in the 9th edition (I don't have the 10th) of Conn's Biological stains. The active coloring matter is called crocin, composed of crocetin and gentobiose. To prepare the stain, the dye is extracted from the crude spice with ethanol. Because saffron is so expensive, the WHO tumor fascicles (in the 1960's) suggested extracting the dye into ethanol using a reflux condenser to achieve maximum yield. This alcohol extract has an obnoxious medicinal smell. A look-alike, safflower (Carthamus tinctorius), sometimes called dyer's saffron or bastard saffron, is odorless and contains a different dye, carthamin or carthamone, chemically unrelated. I have never seen safflower referred to as a histologic stain. Safflower is sometimes referred to as saffron, and I'd be careful not to buy it for histologic use - remember it's odorless. (Safflower is grown commercially as an oil seed.) Saffron was historically grown in France and Spain. It is still grown commercially in Spain, but most of it is grown in India. The traditional histologic designation "safran du G?tinais" referred to the French product, which I think is no longer available. (Saffron was grown in England centuries ago, hence the place name Saffron Walden.) I checked a high-end spice dealer, Penzeys Spices (disclaimer - my wife orders a box of spices from them about once a month), and found saffron for US$10 to 15 a gram, retailed in gram quantities, depending on the source. I suspect it could be ordered from India, perhaps through an Indian grocery store, for less, but I'd want to be awfully careful I was getting Crocus sativus. Apparently lower grades of saffron can be bought in bulk for a dollar or two a gram. The Wikipedia article on saffron is worth reading. According to Wikipedia the coat of arms of Saffron Walden is "Vert within a representation of town walls having two towers and a Gateway between towers Argent three Saffron Flowers issuant from the battlements of the gateway blown and showing the stamens proper And for the Crest On a Wealth of the Colours Upon a Chapeau Gules turned up Ermine a Lion rampant Azure grasping in the dexter paw a representation of the Ancient Mace of the Borough of Saffron Walden proper." Bob Richmond Samurai Pathologist, histoantiquarian and occasional blazoner wannabe Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djamesnz <@t> orcon.net.nz Sun Aug 19 17:37:28 2007 From: djamesnz <@t> orcon.net.nz (Darren James) Date: Sun Aug 19 17:38:04 2007 Subject: [Histonet] Re: Safran du Gatinais In-Reply-To: <200708192102.l7JL2OCq065813@pro12.abac.com> References: <8C9B0B84708AD06-8C8-7ECE@FWM-D19.sysops.aol.com> <200708192102.l7JL2OCq065813@pro12.abac.com> Message-ID: <000301c7e2b1$91befc50$0201010a@Home> Good luck with that. It takes 70,000 - 200,000 flowers to produce 1kg of saffron. The average weight of fresh stigmas is 0.03g per flower, with the dry weight being approx 0.007g per flower. Just a bit of useless trivia to file away for a boring dinner party :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy ruegg Sent: Monday, 20 August 2007 9:06 a.m. To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Safran du Gatinais Being a gardener myself, I was wondering why we don't just grow some of our own Crocus plants, or is the common Crocus plant not the same plant that produces the Saffron from India these days, or perhaps you would have to grow so much of it to yield enough of the stigmas?????? I know Crocus grows in Colorado, even higher than I, I once saw a huge yard full of Crocus plants in Aspen, CO at the end of the summer, of course it would die off during the winter. Is my 5 acres in Colorado big enough, maybe I can grow Crocus as my cash crop? When I lived in California I had a house full of Crocus plants, they are very common there. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Sunday, August 19, 2007 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Safran du Gatinais Lynne Cates in Durham NC asks about buying saffron in bulk for histologic use. Saffron, a culinary spice and coloring agent most familiarly used in paella, consists of the stigmas of the flowers of Crocus sativus. The spice has a very strong odor, and also contains a dye of some histologic interest. Because of its labor-intensive production (do YOU want to spend your days picking the sex organs out of itty bitty flowers?) saffron's extremely expensive. Saffron is used histologically as a connective tissue stain, traditionally in one of the many techniques attributed to Dr. Masson, and in the Movat pentachrome stain. It dyes collagen a yellow-orange color that contrasts subtly with eosin. Saffron has a Colour Index number (75100) and is described in the 9th edition (I don't have the 10th) of Conn's Biological stains. The active coloring matter is called crocin, composed of crocetin and gentobiose. To prepare the stain, the dye is extracted from the crude spice with ethanol. Because saffron is so expensive, the WHO tumor fascicles (in the 1960's) suggested extracting the dye into ethanol using a reflux condenser to achieve maximum yield. This alcohol extract has an obnoxious medicinal smell. A look-alike, safflower (Carthamus tinctorius), sometimes called dyer's saffron or bastard saffron, is odorless and contains a different dye, carthamin or carthamone, chemically unrelated. I have never seen safflower referred to as a histologic stain. Safflower is sometimes referred to as saffron, and I'd be careful not to buy it for histologic use - remember it's odorless. (Safflower is grown commercially as an oil seed.) Saffron was historically grown in France and Spain. It is still grown commercially in Spain, but most of it is grown in India. The traditional histologic designation "safran du G?tinais" referred to the French product, which I think is no longer available. (Saffron was grown in England centuries ago, hence the place name Saffron Walden.) I checked a high-end spice dealer, Penzeys Spices (disclaimer - my wife orders a box of spices from them about once a month), and found saffron for US$10 to 15 a gram, retailed in gram quantities, depending on the source. I suspect it could be ordered from India, perhaps through an Indian grocery store, for less, but I'd want to be awfully careful I was getting Crocus sativus. Apparently lower grades of saffron can be bought in bulk for a dollar or two a gram. The Wikipedia article on saffron is worth reading. According to Wikipedia the coat of arms of Saffron Walden is "Vert within a representation of town walls having two towers and a Gateway between towers Argent three Saffron Flowers issuant from the battlements of the gateway blown and showing the stamens proper And for the Crest On a Wealth of the Colours Upon a Chapeau Gules turned up Ermine a Lion rampant Azure grasping in the dexter paw a representation of the Ancient Mace of the Borough of Saffron Walden proper." Bob Richmond Samurai Pathologist, histoantiquarian and occasional blazoner wannabe Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: 19/08/2007 7:27 a.m. No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: 19/08/2007 7:27 a.m. From ree3 <@t> leicester.ac.uk Mon Aug 20 03:37:43 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Aug 20 03:37:58 2007 Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 In-Reply-To: References: <171741.71965.qm@web50111.mail.re2.yahoo.com> Message-ID: No, it's gum disease and dental caries that vampires are really really concerned about. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate Hilburn Sent: 17 August 2007 17:11 To: Histonet Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 I would think the Transylvanian vampires would be far more concerned about AIDS than cancer. Kate Hilburn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, August 17, 2007 11:28 AM To: Histonet Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07 So, the "undead" of Transylvania have a large enough lobby to get all of Europe against embalming with formaldehyde? ;) In all honesty though, beware the slippery slope of banning something for one use. Our astronaut's safety has become secondary to using inferior materials for environmental purposes. Paula Pierce, HTL(ASCP)HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.edgar <@t> vet.gla.ac.uk Mon Aug 20 03:42:23 2007 From: j.edgar <@t> vet.gla.ac.uk (Julia Edgar) Date: Mon Aug 20 03:42:30 2007 Subject: [Histonet] processing material for paraffin embedding Message-ID: <001101c7e306$07d75550$5aead182@vet.gla.ac.uk> Dear All I have heard two opinions on the best way to treat tissue before processing for paraffin embedding. First is to wash out the paraformaldehyde using phosphate buffer and second is to put straight into alcohol without washing. Your opinions and justifications gratefully received. Thank you Julia From ree3 <@t> leicester.ac.uk Mon Aug 20 03:52:39 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Aug 20 03:52:49 2007 Subject: [Histonet] Formaldehyde Ban - - at funerals ? almost funny. In-Reply-To: References: Message-ID: Well blow me down and there's me thinking all these years that Dame Sylvie Krin was a lady. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 19 August 2007 05:52 To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; 'Amos Brooks' Subject: Re: [Histonet] Formaldehyde Ban - - at funerals ? almost funny. ---Original Message--- > This ban (if it really exists) refers to embalming bodies in funeral > ceremonies. ... Like this? To the sound of mournful music the lead-lined oaken coffin was slowly carried up the aisle and placed on a plinth before the altar. The undertaker, using a stainless steel brace and bit that shone like burnished silver, drilled a hole in the lid and inserted a large plastic funnel. His burly assistant slowly and reverently poured in fifty-four litres of environmentally friendly embalming fluid, warranted free of formaldehyde, while the choir sang six verses of "For all the saints," obscuring the gurglings from the funnel. The undertaker then took a cork, carried to him on a satin cushion by one of the youngest relatives of the deceased. He elegantly plugged the hole in the coffin's lid while his assistant inconspicuously carried away the funnel and the six dozen-size cardboard boxes of empty vodka bottles. After several long speeches and a brief prayer for the soul of the deceased, the coffin containing the newly embalmed body was taken to the crematorium. This deprived the cemetery worms of a free feast and booze-up, but they had no way of knowing that. (Apologies to Sylvie Krin for copying his style but not very well. That's for Brit histonetters and expats who read Private Eye. ) John Kiernan (Both Brit and Canadian) London, Ontario --- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Aug 20 07:33:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 20 07:34:00 2007 Subject: [Histonet] processing material for paraffin embedding In-Reply-To: <001101c7e306$07d75550$5aead182@vet.gla.ac.uk> Message-ID: <609432.84769.qm@web61224.mail.yahoo.com> I always preferred washing in PBS Ren? J. Julia Edgar wrote: Dear All I have heard two opinions on the best way to treat tissue before processing for paraffin embedding. First is to wash out the paraformaldehyde using phosphate buffer and second is to put straight into alcohol without washing. Your opinions and justifications gratefully received. Thank you Julia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From jhr1x <@t> clinmed.gla.ac.uk Mon Aug 20 08:02:00 2007 From: jhr1x <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Mon Aug 20 08:02:13 2007 Subject: [Histonet] processing material for paraffin embedding Message-ID: <004901c7e32a$4c10ced0$1c7ed182@ssd4.gla.ac.uk> Hello Julia I usually go straight to 70% Ethanol without washing. Jim Reilly From innvx <@t> sbcglobal.net Mon Aug 20 08:29:29 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Aug 20 08:29:40 2007 Subject: [Histonet] (no subject) Message-ID: <4867.59826.qm@web82007.mail.mud.yahoo.com> Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining, Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. From kalschev <@t> svm.vetmed.wisc.edu Mon Aug 20 09:07:04 2007 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Mon Aug 20 09:07:18 2007 Subject: [Histonet] Saffron/Sigma Message-ID: <001001c7e333$63219150$c5d76880@vetmed.wisc.edu> Saffron (C.I. 75100) No. S-8381 1 gram bottles SIGMA Chemical Co. St. Louis, MO From PMonfils <@t> Lifespan.org Mon Aug 20 09:38:24 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Aug 20 09:38:36 2007 Subject: [Histonet] processing material for paraffin embedding In-Reply-To: <001101c7e306$07d75550$5aead182@vet.gla.ac.uk> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CC8@LSRIEXCH1.lsmaster.lifespan.org> I have done it both ways, and have never seen any difference either in morphology or in staining. So of course I now skip the unnecessary step and go straight into the alcohol series. If I was going to wash, I would just use water. There is no need to use buffer on already fixed tissues. From kmerriam2003 <@t> yahoo.com Mon Aug 20 10:21:37 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Aug 20 10:21:48 2007 Subject: [Histonet] IF staining of mast cells and fibroblasts Message-ID: <388072.97527.qm@web50306.mail.re2.yahoo.com> Hello all, I was wondering if anyone knew of specific markers for mast cells and fibroblasts. I need markers for IF and IHC (not special stains) that will stain only mast cells and only fibroblasts. We have a few markers in mind, but we are not sure if they are specific only to those types of cells and will not cross-react with some other cell type. Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz From bhewlett <@t> cogeco.ca Mon Aug 20 10:29:08 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Aug 20 10:29:46 2007 Subject: [Histonet] IF staining of mast cells and fibroblasts References: <388072.97527.qm@web50306.mail.re2.yahoo.com> Message-ID: <001e01c7e33e$dfa46940$6500a8c0@mainbox> Kim, In which species? For human mast cells in FPPE sections, we used Mast cell tryptase for IHC. Bryan ----- Original Message ----- From: "Kim Merriam" To: "Histonet" Sent: Monday, August 20, 2007 11:21 AM Subject: [Histonet] IF staining of mast cells and fibroblasts Hello all, I was wondering if anyone knew of specific markers for mast cells and fibroblasts. I need markers for IF and IHC (not special stains) that will stain only mast cells and only fibroblasts. We have a few markers in mind, but we are not sure if they are specific only to those types of cells and will not cross-react with some other cell type. Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Mon Aug 20 10:29:05 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Aug 20 10:29:52 2007 Subject: [Histonet] Re: Safran du Gatinais Message-ID: Crocus sativus needs a warm climate such as the Mediterranean or California. It's stated to be hardy in N. American Zones 6-7. Only the stigma contains the saffron carotenoids - about 5mg per flower, so you'll need to grow a lot of them. I don't know whether the ordinary garden crocuses of cooler climes contain enough saffron in their stigmas to be worth bothering with. John Kiernan London, Canada --- ----- Original Message ----- From: patsy ruegg Date: Sunday, August 19, 2007 17:04 Subject: RE: [Histonet] Re: Safran du Gatinais To: rsrichmond@aol.com, histonet@lists.utsouthwestern.edu > Being a gardener myself, I was wondering why we don't just grow > some of our > own Crocus plants, or is the common Crocus plant not the same > plant that > produces the Saffron from India these days, or perhaps you would > have to > grow so much of it to yield enough of the stigmas?????? I > know Crocus grows > in Colorado, even higher than I, I once saw a huge yard full of Crocus > plants in Aspen, CO at the end of the summer, of course it would > die off > during the winter. Is my 5 acres in Colorado big enough, maybe I > can grow > Crocus as my cash crop? When I lived in California I had a > house full of > Crocus plants, they are very common there. > Best regards, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > rsrichmond@aol.com > Sent: Sunday, August 19, 2007 2:40 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Safran du Gatinais > > Lynne Cates in Durham NC asks about buying saffron in bulk > for histologic > use. > > Saffron, a culinary spice and coloring agent most familiarly > used in paella, > consists of the stigmas of the flowers of Crocus sativus. The > spice has a > very strong odor, and also contains a dye of some histologic interest. > Because of its labor-intensive production (do YOU want to spend > your days > picking the sex organs out of itty bitty flowers?) saffron's extremely > expensive. > > Saffron is used histologically as a connective tissue stain, > traditionallyin one of the many techniques attributed to Dr. > Masson, and in the Movat > pentachrome stain. It dyes collagen a yellow-orange color that > contrastssubtly with eosin. > > Saffron has a Colour Index number (75100) and is described in > the 9th > edition (I don't have the 10th) of Conn's Biological stains. The > activecoloring matter is called crocin, composed of crocetin and > gentobiose. > To prepare the stain, the dye is extracted from the crude spice with > ethanol. Because saffron is so expensive, the WHO tumor > fascicles (in the > 1960's) suggested extracting the dye into ethanol using a reflux > condenserto achieve maximum yield. This alcohol extract has an > obnoxious medicinal > smell. > > A look-alike, safflower (Carthamus tinctorius), sometimes called > dyer'ssaffron or bastard saffron, is odorless and contains a > different dye, > carthamin or carthamone, chemically unrelated. I have never seen > safflowerreferred to as a histologic stain. Safflower is > sometimes referred to as > saffron, and I'd be careful not to buy it for histologic use - > remember it's > odorless. (Safflower is grown commercially as an oil seed.) > > Saffron was historically grown in France and Spain. It is still grown > commercially in Spain, but most of it is grown in India. The > traditionalhistologic designation "safran du G?tinais" referred > to the French product, > which I think is no longer available. (Saffron was grown in England > centuries ago, hence the place name Saffron Walden.) > > I checked a high-end spice dealer, Penzeys Spices (disclaimer - > my wife > orders a box of spices from them about once a month), and found > saffron for > US$10 to 15 a gram, retailed in gram quantities, depending on > the source. I > suspect it could be ordered from India, perhaps through an > Indian grocery > store, for less, but I'd want to be awfully careful I was > getting Crocus > sativus. Apparently lower grades of saffron can be bought in > bulk for a > dollar or two a gram. > > The Wikipedia article on saffron is worth reading. > > According to Wikipedia the coat of arms of Saffron Walden is > "Vert within a > representation of town walls having two towers and a Gateway > between towers > Argent three Saffron Flowers issuant from the battlements of the > gatewayblown and showing the stamens proper And for the Crest On > a Wealth of the > Colours Upon a Chapeau Gules turned up Ermine a Lion rampant > Azure grasping > in the dexter paw a representation of the Ancient Mace of the > Borough of > Saffron Walden proper." > > Bob Richmond > Samurai Pathologist, histoantiquarian and occasional blazoner wannabe > Knoxville TN > > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about > what's free from > AOL at AOL.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Mon Aug 20 10:30:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Aug 20 10:30:34 2007 Subject: [Histonet] growing gram pos and neg In-Reply-To: <9b07f78c93a361469c69ae3b3c131e69@mail2.lcpath.com> References: <9b07f78c93a361469c69ae3b3c131e69@mail2.lcpath.com> Message-ID: <6.0.0.22.1.20070820090641.01bf1a00@gemini.msu.montana.edu> Jennifer, There is a wonderful publication on making a combined Gram negative and Gram positive control in J of Histotechnology, December 2006. The author is Kristine J. Vaughn (do not have email contact here) but I will CC to my home email so I can put you in contact with her. She has reprints available, and will be delighted to send you one. Her method is simple and very effective, plus addresses biosafety issues with human tissue use. If you are a member of NSH, you can contact JOH and request this reprint free of charge too. Go to the NSH website, then click on JOH for this request. At 04:57 PM 8/17/2007, you wrote: >Hello all, > >I would like to hear your ideas on the best way to grow gram positive >and negative organisms in tissue. It would have to be tissue that is not >fixed so, what do you use? Did you grow the organisms, inject into >tissue and then put into incubator? or do you grow organisms on agar and >place tissue on it and incubate? or in a broth? Thank you for your >input. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From PMonfils <@t> Lifespan.org Mon Aug 20 10:30:23 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Aug 20 10:30:39 2007 Subject: [Histonet] growing gram pos and neg In-Reply-To: <9b07f78c93a361469c69ae3b3c131e69@mail2.lcpath.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CC9@LSRIEXCH1.lsmaster.lifespan.org> In the past I have made such gram controls, but I preferred to obtain the organisms already grown, rather than trying to grow them myself. I ordered cultures of gram+ and gram- organisms, grown on broth, from a biological supply company. Then I just spun them down to concentrate the organisms, injected the concentrate into a piece of lung, and dropped the tissue into formalin. Push the needle almost all the way through the piece of tissue, then inject continuously as you withdraw it. I used lung because there is plenty of microscopic open space into which the broth can be injected, without doing any great damage to the tissue structure. Injecting into a more solid tissue like liver doesn't work well because there is nowhere for the injected fluid to go. A space has to be torn open by the pressure of injection. I would usually inject two different gram+ organisms, mixed together, into one piece of lung. Then two gram- organisms mixed, into another piece of lung. After fixation I would cut the injected tissue into small cubes, and embed a gram+ cube and a gram- cube side by side in a block. For gram+ I liked to use a Bacillus, such as B. cereus or B. subtilus, mixed with a Staphylococcus like S. epidermidis. This provides two different shapes of organisms, both gram+. For gram- I used Escherichia coli mixed with Neisseria subflava. My source for the organisms was http://www.ctvalleybio.com/ The cost is less than $10 per culture and you can make enough good control blocks to last forever. From je2 <@t> sanger.ac.uk Mon Aug 20 11:02:44 2007 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Mon Aug 20 11:02:56 2007 Subject: [Histonet] Dmetrix scanner Message-ID: <0526C4B4E593154B86E6A8651913C28D013E0607@exchsrv2.internal.sanger.ac.uk> Hello, I need some help. We have a Dmetrix scanner and we have some problems. The slides are not loading properly. Does anyone have the same problem? Can you tell me which slides are you using? Regards Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Cambridge, UK CB10 1SA -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. From liz <@t> premierlab.com Mon Aug 20 11:20:09 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Aug 20 11:20:30 2007 Subject: [Histonet] IF staining of mast cells and fibroblasts In-Reply-To: <6C9FDDB7C80D49AA8765A44DBDCC7D0E@PremierLab.local> References: <6C9FDDB7C80D49AA8765A44DBDCC7D0E@PremierLab.local> Message-ID: Kim Mast cell tryptase works in mice also, so I suspect it might work in = other species. For fibroblasts there is a marker that works in rat its = mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody = 6-9H6 (AF5110-1) from Acris.=20 Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim = Merriam Sent: Monday, August 20, 2007 9:46 AM To: Histonet Subject: [Histonet] IF staining of mast cells and fibroblasts Hello all, I was wondering if anyone knew of specific markers for mast cells and = fibroblasts. I need markers for IF and IHC (not special stains) that = will stain only mast cells and only fibroblasts. We have a few markers = in mind, but we are not sure if they are specific only to those types of = cells and will not cross-react with some other cell type. Thanks in advance, Kim =20 Kim Merriam, MA, HT(ASCP) Cambridge, MA =20 _________________________________________________________________________= ___________ Got a little couch potato?=20 Check out fun summer activities for kids. http://search.yahoo.com/search?fr=3Doni_on_mail&p=3Dsummer+activities+for= +kids&cs=3Dbz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: = 8/19/2007 7:27 AM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: = 8/19/2007 7:27 AM =20 From b-frederick <@t> northwestern.edu Mon Aug 20 11:25:09 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 20 11:25:30 2007 Subject: [Histonet] Re: Safran du Gatinais In-Reply-To: <8C9B0B84708AD06-8C8-7ECE@FWM-D19.sysops.aol.com> Message-ID: <003a01c7e346$b0067ea0$d00f7ca5@lurie.northwestern.edu> Rowley biochemical sells Alcoholic saffron as part of a Movat's kit as well as stand alone. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Sunday, August 19, 2007 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Safran du Gatinais Lynne Cates in Durham NC asks about buying saffron in bulk for histologic use. Saffron, a culinary spice and coloring agent most familiarly used in paella, consists of the stigmas of the flowers of Crocus sativus. The spice has a very strong odor, and also contains a dye of some histologic interest. Because of its labor-intensive production (do YOU want to spend your days picking the sex organs out of itty bitty flowers?) saffron's extremely expensive. Saffron is used histologically as a connective tissue stain, traditionally in one of the many techniques attributed to Dr. Masson, and in the Movat pentachrome stain. It dyes collagen a yellow-orange color that contrasts subtly with eosin. Saffron has a Colour Index number (75100) and is described in the 9th edition (I don't have the 10th) of Conn's Biological stains. The active coloring matter is called crocin, composed of crocetin and gentobiose. To prepare the stain, the dye is extracted from the crude spice with ethanol. Because saffron is so expensive, the WHO tumor fascicles (in the 1960's) suggested extracting the dye into ethanol using a reflux condenser to achieve maximum yield. This alcohol extract has an obnoxious medicinal smell. A look-alike, safflower (Carthamus tinctorius), sometimes called dyer's saffron or bastard saffron, is odorless and contains a different dye, carthamin or carthamone, chemically unrelated. I have never seen safflower referred to as a histologic stain. Safflower is sometimes referred to as saffron, and I'd be careful not to buy it for histologic use - remember it's odorless. (Safflower is grown commercially as an oil seed.) Saffron was historically grown in France and Spain. It is still grown commercially in Spain, but most of it is grown in India. The traditional histologic designation "safran du G?tinais" referred to the French product, which I think is no longer available. (Saffron was grown in England centuries ago, hence the place name Saffron Walden.) I checked a high-end spice dealer, Penzeys Spices (disclaimer - my wife orders a box of spices from them about once a month), and found saffron for US$10 to 15 a gram, retailed in gram quantities, depending on the source. I suspect it could be ordered from India, perhaps through an Indian grocery store, for less, but I'd want to be awfully careful I was getting Crocus sativus. Apparently lower grades of saffron can be bought in bulk for a dollar or two a gram. The Wikipedia article on saffron is worth reading. According to Wikipedia the coat of arms of Saffron Walden is "Vert within a representation of town walls having two towers and a Gateway between towers Argent three Saffron Flowers issuant from the battlements of the gateway blown and showing the stamens proper And for the Crest On a Wealth of the Colours Upon a Chapeau Gules turned up Ermine a Lion rampant Azure grasping in the dexter paw a representation of the Ancient Mace of the Borough of Saffron Walden proper." Bob Richmond Samurai Pathologist, histoantiquarian and occasional blazoner wannabe Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tina.hammel <@t> usafa.af.mil Mon Aug 20 11:25:47 2007 From: tina.hammel <@t> usafa.af.mil (Hammel Tina M SSgt 10 MDSS/SGSC) Date: Mon Aug 20 11:27:55 2007 Subject: [Histonet] Breast Tissue Message-ID: We have been having problems with breast tissue being fatty and under processed. At the other Lab I worked we had a separate processor for fatty tissue. The Lab I'm at now only has one tissue processor for everything; we have another processor that is not being used. I brought up the idea of using that one for fatty tissue, but they want me to get other opinions on the idea. So I need ideas on how other Labs are processing their fatty tissue. V/R Tina From igor.deyneko <@t> gmail.com Mon Aug 20 12:09:07 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Aug 20 12:09:26 2007 Subject: [Histonet] Myofibroblasts VS Fibrobalsts Message-ID: <35e16a770708201009n33a9df8dm1b1ae7dac212c619@mail.gmail.com> Hello Histonetters! I was wondering does anyone know a good way to differentiate between human myofibroblasts and fibroblasts. Is there a specific stain or an antibody for IHC??? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge,MA From AGrobe2555 <@t> aol.com Mon Aug 20 12:48:28 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Aug 20 12:48:47 2007 Subject: [Histonet] Myofibroblasts VS Fibroblasts Message-ID: Igor, You have asked the $1,000,000 question. I've got a couple references that may help. Schurch, W., Seemayer, T. A., Gabbiani, G. The myofibroblast: a quarter century after its discovery. Am J Surg Pathol 22(2), 1998, pp 141-147 Gabbiani, G. Evolution and clinical implications of the myofibroblast concept. Cardiovasc Res 38(3), 1998, pp 545-548 There are others that are more recent, but these were the ones that came to mind. I'll try to find more... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From b-frederick <@t> northwestern.edu Mon Aug 20 12:59:01 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 20 12:59:23 2007 Subject: [Histonet] IF staining of mast cells and fibroblasts In-Reply-To: Message-ID: <004e01c7e353$cd3fce10$d00f7ca5@lurie.northwestern.edu> Mast cell tryptase works fine on human 1:8000. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Monday, August 20, 2007 11:20 AM To: Kim Merriam; Histonet Subject: RE: [Histonet] IF staining of mast cells and fibroblasts Kim Mast cell tryptase works in mice also, so I suspect it might work in other species. For fibroblasts there is a marker that works in rat its mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody 6-9H6 (AF5110-1) from Acris. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, August 20, 2007 9:46 AM To: Histonet Subject: [Histonet] IF staining of mast cells and fibroblasts Hello all, I was wondering if anyone knew of specific markers for mast cells and fibroblasts. I need markers for IF and IHC (not special stains) that will stain only mast cells and only fibroblasts. We have a few markers in mind, but we are not sure if they are specific only to those types of cells and will not cross-react with some other cell type. Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ________________________________________________________________________ ____________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+ki ds&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: 8/19/2007 7:27 AM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: 8/19/2007 7:27 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Mon Aug 20 13:19:25 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Aug 20 13:19:46 2007 Subject: [Histonet] IF staining of mast cells and fibroblasts Message-ID: <000c01c7e356$a4224370$4101a8c0@carlba65530bda> Liz....I'd be most grateful for source/cat. number for the mouse mast cell tryptase-reactive Ab mentioned. Thanks for the rat-fibroblast marker info! I assume that these are FFPW section -reactive, after Ag retrieval? Which method works for you? Carl From hodges420 <@t> msn.com Mon Aug 20 13:23:33 2007 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Mon Aug 20 13:23:53 2007 Subject: [Histonet] microwave reproducibility Message-ID: accually you do three containers of fluid Bob at three different times. use water first and check all three then use alcohol and do all three example 30 sec h2o 45 sec h2o 60 sec h2o then do alcohal at the same times this proves if there are hot spots and reproducablitity through out your microwave Tere Hodges >From: Robert Chiovetti >To: GLORIA MUNOZ , histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] microwave reproducibility >Date: Sat, 18 Aug 2007 10:28:37 -0700 (PDT) > >Gloria, > >I agree with Rene, heating a known volume of water in the microwave for >about 60 seconds and measuring the change in temperature of the water is >probably the best way to document the reproducibility of the microwave. >Just measure the temperature of the water before you heat it, then gently >stir the water and measure the temperature again after you've heated it. >Subtract the two and record the increase in temperature. > >You'll probably have to experiment with the volume of water you heat, since >you'll want to heat the water to a point somewhere below its boiling point. > It would probably be a good idea to make about 3 tests for each power >level of the oven, then calculate an average of the 3 readings. Begin each >test with water that's near room temperature. A good starting point would >probably be to heat about 500 ml or 1 L of water for 60 seconds and see >what you get. You can expect some variation in the results, probably a >degree or two in temperature, but microwave ovens are remarkably >reproducible in this respect. > >Heating a volume of water for a set time is the first step in calculating >the output (in Watts) of your microwave, and there are formulas to take the >results to the next step and actually calculate the wattage of the oven. >The full calculations probably aren't necessary for day-to-day operation, >though. > >With age, the magnetron in the oven (the part that emits the microwaves) >can gradually become less efficient. In that case you would notice less of >an increase in temperature of the water. You'll also have records to show >whether the magnetron is working properly during normal operation. Factors >like total available power, the condition of the high voltage transformer, >the control circuits for the magnetron and lots of other things could >affect the microwave's output, so it's a good idea to do this test on a >"regular" basis. That probably means weekly or monthly, I don't know for >sure. Maybe there are others on Histonet who have some suggestions here. > >Just be sure to always heat the same volume of water for the same length of >time, and you'll have the records you need. > >Hope this helps. > >Cheers, > >Bob > > >Robert (Bob) Chiovetti, Ph.D. >Southwest Precision Instruments >www.swpinet.com >The Desert Southwest's Microscopy Resource >132 North Elster Drive >Tucson, AZ 85710-3212 >Tel./Fax 520-546-4986 >Member, Arizona Small Business Association >(www.asba.com) > >----- Original Message ---- >From: GLORIA MUNOZ >To: histonet@lists.utsouthwestern.edu >Sent: Friday, August 17, 2007 8:36:50 AM >Subject: [Histonet] microwave reproducibility > >Hi HistoNetters: > Just wondering if any of you have a procedure to share on microwave >reproducibility for a store-bought, non-medical microwave. We had our CAP >inspection recently and were asked to come up with a method to test the >microwave. The inspector recommended heating distilled water for one >minute at each of the three different power levels and documenting the >results. Does anyone know of another, maybe better, way? > Thanks a lot, enjoy reading your entries, and hope you all have a >restful weekend...........gloria >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > >____________________________________________________________________________________ >Choose the right car based on your needs. Check out Yahoo! Autos new Car >Finder tool. >http://autos.yahoo.com/carfinder/ >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Learn.Laugh.Share. Reallivemoms is right place! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us From doug <@t> ppspath.com Mon Aug 20 14:35:49 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Aug 20 13:37:13 2007 Subject: [Histonet] Grossing Question Message-ID: It has been brought to my attention that some facilities may be using non PA's to gross the larger surgical specimens (i.e. uterus, colon, and breast). These "grossers" fall under the CLIA guidelines for "high complexity testing". Is this "legit"? I thought that there was a specimen type or complexity that these "grossers" should not even be doing. If this is the case then doesn't it devalue the PA? Not that I think it does. Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From CBark <@t> memorialcare.org Mon Aug 20 13:40:59 2007 From: CBark <@t> memorialcare.org (Christine Bark) Date: Mon Aug 20 13:41:53 2007 Subject: [Histonet] RE: Breast tissue In-Reply-To: <6AD716F40KC810136-01@emf2.memorialcare.org> Message-ID: Prior to getting a second processor, we held fatty cases over night in pen-fix (an alcohol-formalin solution). Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org We have been having problems with breast tissue being fatty and under processed. At the other Lab I worked we had a separate processor for fatty tissue. The Lab I'm at now only has one tissue processor for everything; we have another processor that is not being used. I brought up the idea of using that one for fatty tissue, but they want me to get other opinions on the idea. So I need ideas on how other Labs are processing their fatty tissue. V/R Tina ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 45, Issue 29 **************************************** ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From innvx <@t> sbcglobal.net Mon Aug 20 15:04:10 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Aug 20 15:04:29 2007 Subject: [Histonet] Animal IHC workshop at Duke university Message-ID: <561008.16386.qm@web82012.mail.mud.yahoo.com> Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining and Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. From jwatson <@t> gnf.org Mon Aug 20 15:29:12 2007 From: jwatson <@t> gnf.org (James Watson) Date: Mon Aug 20 15:29:25 2007 Subject: [Histonet] Animal IHC workshop at Duke university In-Reply-To: <561008.16386.qm@web82012.mail.mud.yahoo.com> References: <561008.16386.qm@web82012.mail.mud.yahoo.com> Message-ID: I do not know this company, but I do believe that this is blatant advertising of your products under the false pretense of an animal IHC staining workshop. I did not think that the Histonet was to be used by companies for advertising. I do not see other companies that give CEU approved courses advertising on the Histonet, why should Innovex be allowed to. Just my opinion on a busy Monday. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX BIOSCIENCES Sent: Monday, August 20, 2007 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal IHC workshop at Duke university Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining and Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Mon Aug 20 15:46:21 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Mon Aug 20 15:43:53 2007 Subject: [Histonet] Animal IHC workshop at Duke university In-Reply-To: Message-ID: <000601c7e36b$2af65120$3601a8c0@brownpathology.net> That's what the delete button is for.... if I were doing much animal tissue, I'd like to know when a free workshop with CEU's was close by.... regardless of commercial content. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Monday, August 20, 2007 3:29 PM To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university I do not know this company, but I do believe that this is blatant advertising of your products under the false pretense of an animal IHC staining workshop. I did not think that the Histonet was to be used by companies for advertising. I do not see other companies that give CEU approved courses advertising on the Histonet, why should Innovex be allowed to. Just my opinion on a busy Monday. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX BIOSCIENCES Sent: Monday, August 20, 2007 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal IHC workshop at Duke university Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining and Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrea.stritmatter <@t> mdsinc.com Mon Aug 20 15:58:27 2007 From: andrea.stritmatter <@t> mdsinc.com (Andrea Stritmatter) Date: Mon Aug 20 15:58:47 2007 Subject: [Histonet] Open Positions Message-ID: We have two histology positions open at MDS. The company is just north of Seattle, WA. We are hiring a Histologist (RA level II) and a Histomorphometrist. Below are the job descriptions. Please apply online. If you have any questions, please feel free to contact me directly. MDS Pharma Services, Efficacy-Pharmacology, is a therapeutically focused contract research organization that specializes in bone and central nervous system (CNS) studies. We are currently seeking an experienced Histologist to participate in pre-clinical research programs. Responsibilities will include hard and soft tissue processing, embedding, thin sectioning, staining, and the development and validation of laboratory techniques in support of client-sponsored research programs. Specific experience with mouse tissue histology and orthopedic histological techniques is highly desired. Qualified applicants will be able to perform all aspects of histology studies with minimal supervision. Top candidates will possess a Bachelor's degree, a HT/HTL certification, and/or 3+ years of directly related histology experience. Candidates with prior experience working in a GLP compliant laboratory will be considered superior. MDS Pharma Services offers an energetic and challenging research environment with a competitive compensation and benefits package including equity participation. Interested applicants can apply via the MDS Pharma Services website located at www.mdsps.com. MDS Pharma Services, Efficacy-Pharmacology, is a therapeutically focused contract research organization that specializes in bone and central nervous system (CNS) studies. We are currently seeking an experienced Histomorphometrist to participate in pre-clinical research programs. Responsibilities will include performing image analysis of histologically prepared bone samples to include variable parameters and provide imaging reports. The ideal candidate will have a Master's degree in a scientific discipline with 2+ years experience in bone histology/histomorphometry or a Bachelor's degree in a scientific discipline with 5+ years experience in bone histology/histomorphometry. Experience with OsteoMeasure and/or Bioguant is ideal. Candidates with prior experience working in a GLP compliant laboratory will be considered superior. MDS Pharma Services offers an energetic and challenging research environment with a competitive compensation and benefits package including equity participation. Interested applicants can apply via the MDS Pharma Services website located at www.mdsps.com. Andrea Stritmatter, HTL(ASCP) Supervisor, Histology and Imaging MDS Pharma Services 425-424-2622 From Anthony.Johnson <@t> leica-microsystems.com Mon Aug 20 16:01:05 2007 From: Anthony.Johnson <@t> leica-microsystems.com (Anthony.Johnson@leica-microsystems.com) Date: Mon Aug 20 16:02:58 2007 Subject: [Histonet] Johnson, Anthony is out of the office. Message-ID: I will be out of the office starting 08/20/2007 and will not return until 08/22/2007. If your message is urgent please contact Luanda Diggs in our Customer Care dept on 847 405 7052. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From shive003 <@t> umn.edu Mon Aug 20 16:04:25 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Aug 20 16:04:39 2007 Subject: Fw: [Histonet] Animal IHC workshop at Duke university Message-ID: <02bb01c7e36d$b0ef1f30$a1065486@auxs.umn.edu> I actually attended this workshop when it was given at a Tri-State meeting last spring, and I agree with James' remarks below. Since that time I've received dozens of workshop notices from Innovex, both directly and through the Histonet, advertising subsequent dates and places. I wrote to Innovex, asking to be taken off their email list (which they promptly did). I also wrote to Histonet about it at that time as well, asking them to look into the appropriateness of advertising Innovex's workshops here on our site, but I see that the workshop notices are still being forwarded to all members. I don't mind being approached individually by companies, but I really don't think that the Histonet should be used in this manner. Just my two cents' worth. Jan Shivers Section Head IHC/Histo/EM UMN Vet Diag Lab St. Paul, MN ----- Original Message ----- From: "James Watson" To: "INNOVEX BIOSCIENCES" ; Sent: Monday, August 20, 2007 3:29 PM Subject: RE: [Histonet] Animal IHC workshop at Duke university I do not know this company, but I do believe that this is blatant advertising of your products under the false pretense of an animal IHC staining workshop. I did not think that the Histonet was to be used by companies for advertising. I do not see other companies that give CEU approved courses advertising on the Histonet, why should Innovex be allowed to. Just my opinion on a busy Monday. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX BIOSCIENCES Sent: Monday, August 20, 2007 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal IHC workshop at Duke university Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining and Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Mon Aug 20 16:11:33 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Aug 20 16:11:44 2007 Subject: [Histonet] Animal IHC workshop at Duke university. . In-Reply-To: Message-ID: <5CB39BCA5724F349BCB748675C6CA1A21454356B@SJMEMXMB02.stjude.sjcrh.local> What is the difference between advertising a workshop given by a company at a university and advertising a workshop given by a company at NSH????? Some of the best workshops I've attended have been workshops given by a company. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Monday, August 20, 2007 3:29 PM To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university. I do not know this company, but I do believe that this is blatant advertising of your products under the false pretense of an animal IHC staining workshop. I did not think that the Histonet was to be used by companies for advertising. I do not see other companies that give CEU approved courses advertising on the Histonet, why should Innovex be allowed to. Just my opinion on a busy Monday. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX BIOSCIENCES Sent: Monday, August 20, 2007 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal IHC workshop at Duke university Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining and Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From innvx <@t> sbcglobal.net Mon Aug 20 16:18:57 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Aug 20 16:19:07 2007 Subject: [Histonet] on the subject of workshops Message-ID: <561012.31486.qm@web82014.mail.mud.yahoo.com> This is an educational CEU approved workshop that covers all academics of IHC staining workshop, this is not an autostainer demo workshop and there are a lot of people out there that need the knowledge of staining animal tissue IHC and we are usually invited by major medical center to conduct this workshop. We at Innovex feel sorry that you feel this way , we simply posted an announcement for a workshop and since you have not been to one of our workshops ...it is very strange that you have already rated it as an advertisement for products. You do not have all the facts and are jumping to conclusion. By the way Innovex has been around for 14 years. Sherry Innovex Biosciences From innvx <@t> sbcglobal.net Mon Aug 20 17:37:01 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Aug 20 17:37:14 2007 Subject: [Histonet] workshops Message-ID: <87319.31555.qm@web82002.mail.mud.yahoo.com> YAHOO.Shortcuts.hasSensitiveText = true; YAHOO.Shortcuts.sensitivityType = ["adult"]; YAHOO.Shortcuts.doUlt = false; YAHOO.Shortcuts.location = "us"; YAHOO.Shortcuts.lang = "us"; YAHOO.Shortcuts.document_id = 0; YAHOO.Shortcuts.document_type = ""; YAHOO.Shortcuts.document_title = ""; YAHOO.Shortcuts.document_publish_date = ""; YAHOO.Shortcuts.document_author = ""; YAHOO.Shortcuts.document_url = ""; YAHOO.Shortcuts.document_tags = ""; YAHOO.Shortcuts.annotationSet = { "lw_1187649239_0": { "text": "Iowa", "extended": 0, "startchar": 194, "endchar": 197, "start": 194, "end": 197, "extendedFrom": "", "predictedCategory": "PLACE", "predictionProbability": "0.98101", "weight": 0.35, "type": ["shortcuts:/us/instance/place/destination", "shortcuts:/us/instance/place/us/state"], "category": ["PLACE"], "context": " all of them to prove that is not so...and about Iowa person with no one name attached to it...we" }, "lw_1187649239_1": { "text": "Iowa", "extended": 0, "startchar": 270, "endchar": 273, "start": 270, "end": 273, "extendedFrom": "", "predictedCategory": "PLACE", "predictionProbability": "0.98315", "weight": 0.35, "type": ["shortcuts:/us/instance/place/destination", "shortcuts:/us/instance/place/us/state"], "category": ["PLACE"], "context": " attached to it...we have never sent a flyer to Iowa for workshops ...we gave that workshop through", "metaData": { "geoArea": "145523", "geoCountry": "United States", "geoIsoCountryCode": "US", "geoLocation": "(-93.3899, 41.938221)", "geoName": "Iowa", "geoPlaceType": "State", "geoState": "Iowa", "geoStateCode": "IA", "type": "shortcuts:/us/instance/place/us/state" } }, "lw_1187649239_2": { "text": "Iowa", "extended": 0, "startchar": 432, "endchar": 435, "start": 432, "end": 435, "extendedFrom": "", "predictedCategory": "PLACE", "predictionProbability": "0.983872", "weight": 0.35, "type": ["shortcuts:/us/instance/place/destination", "shortcuts:/us/instance/place/us/state"], "category": ["PLACE"], "context": " the society ...we have never sent a flyer to Iowa subsequently. I wish you good luck with Dave" }, "lw_1187649239_3": { "text": "California", "extended": 0, "startchar": 627, "endchar": 636, "start": 627, "end": 636, "extendedFrom": "", "predictedCategory": "PLACE", "predictionProbability": "0.994817", "weight": 0.35, "type": ["shortcuts:/us/instance/place/destination", "shortcuts:/us/instance/place/us/state"], "category": ["PLACE"], "context": " ...Bio care sent four of their people to California meeting workshop which by the way Invokes was" }, "lw_1187649239_4": { "text": "jwatson@gnf.org", "extended": 0, "startchar": 842, "endchar": 856, "start": 842, "end": 856, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": " President Innovex Biosciences James Watson jwatson@gnf.org wrote: By saying that I do not know" }, "lw_1187649239_5": { "text": "Duke university", "extended": 0, "startchar": 5760, "endchar": 5774, "start": 5760, "end": 5774, "extendedFrom": "", "predictedCategory": "ORGANIZATION", "predictionProbability": "0.864067", "weight": 0.595623, "type": ["shortcuts:/us/instance/organization"], "category": ["ORGANIZATION"], "context": " RE: [Histonet] Animal IHC workshop at Duke university This is an educational CEU approved" }, "lw_1187649239_6": { "text": "jwatson@gnf.org", "extended": 0, "startchar": 6799, "endchar": 6813, "start": 6799, "end": 6813, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": " Sherry Innovex Biosciences James Watson jwatson@gnf.org wrote: I do not know this company, but I do" }, "lw_1187649239_7": { "text": "858-332-4647", "extended": 0, "startchar": 7500, "endchar": 7511, "start": 7500, "end": 7511, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/phone_number/us"], "category": ["IDENTIFIER"], "context": " of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original" }, "lw_1187649239_8": { "text": "858-812-1915", "extended": 0, "startchar": 7520, "endchar": 7531, "start": 7520, "end": 7531, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/phone_number/us"], "category": ["IDENTIFIER"], "context": " Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original" }, "lw_1187649239_9": { "text": "jwatson@gnf.org", "extended": 0, "startchar": 7536, "endchar": 7550, "start": 7536, "end": 7550, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": " Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original" }, "lw_1187649239_10": { "text": "histonet-bounces@lists.utsouthwestern.edu", "extended": 0, "startchar": 7596, "endchar": 7636, "start": 7596, "end": 7636, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": " Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.ed" }, "lw_1187649239_11": { "text": "histonet-bounces@lists.utsouthwestern.edu", "extended": 0, "startchar": 7649, "endchar": 7689, "start": 7649, "end": 7689, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": "histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX BIOSCIENCES Sent: Monday," }, "lw_1187649239_12": { "text": "Histonet@lists.utsouthwestern.edu", "extended": 0, "startchar": 7776, "endchar": 7808, "start": 7776, "end": 7808, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": " Monday, August 20, 2007 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal IHC workshop at Duke" }, "lw_1187649239_13": { "text": "Duke university", "extended": 0, "startchar": 7856, "endchar": 7870, "start": 7856, "end": 7870, "extendedFrom": "", "predictedCategory": "ORGANIZATION", "predictionProbability": "0.867076", "weight": 0.595623, "type": ["shortcuts:/us/instance/organization"], "category": ["ORGANIZATION"], "context": " [Histonet] Animal IHC workshop at Duke university Animal IHC staining workshop, Duke university," }, "lw_1187649239_14": { "text": "Duke university", "extended": 0, "startchar": 7909, "endchar": 7923, "start": 7909, "end": 7923, "extendedFrom": "", "predictedCategory": "ORGANIZATION", "predictionProbability": "0.914801", "weight": 0.595623, "type": ["shortcuts:/us/instance/organization"], "category": ["ORGANIZATION"], "context": " Duke university Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU" }, "lw_1187649239_15": { "text": "innvx.com", "extended": 0, "startchar": 8189, "endchar": 8197, "start": 8189, "end": 8197, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/place/virtual/web_site"], "category": ["IDENTIFIER"], "context": " and under 2 hours. Register on line at innvx.com or call 1-800-622-7808." }, "lw_1187649239_16": { "text": "1-800-622-7808", "extended": 0, "startchar": 8211, "endchar": 8224, "start": 8211, "end": 8224, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/phone_number/us"], "category": ["IDENTIFIER"], "context": " 2 hours. Register on line at innvx.com or call 1-800-622-7808." }, "lw_1187649239_17": { "text": "Histonet@lists.utsouthwestern.edu", "extended": 0, "startchar": 8311, "endchar": 8343, "start": 8311, "end": 8343, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/email_address"], "category": ["IDENTIFIER"], "context": " mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo" }, "lw_1187649239_18": { "text": "http://lists.utsouthwestern.edu/mailman/listinfo/histonet", "extended": 0, "startchar": 8348, "endchar": 8404, "start": 8348, "end": 8404, "extendedFrom": "", "predictedCategory": "", "predictionProbability": "0", "weight": 1, "type": ["shortcuts:/us/instance/identifier/URL"], "category": ["IDENTIFIER"], "context": " mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet " } }; YAHOO.Shortcuts.overlaySpaceId = "97546169"; YAHOO.Shortcuts.hostSpaceId = "97546168"; Innovex workshop flyers have never contained products name like Stat-Q or Histo-Stat ...or any other products...we have all of them to prove that is not so...and about Iowa person with no one name attached to it...we have never sent a flyer to Iowa for workshops ...we gave that workshop through state society upon invitation and they registered people through the society ...we have never sent a flyer to Iowa subsequently. I wish you good luck with Dave Tacha and Biocare on your future endeavors...I used to work with Dave...tell him Zara said hello....FYI ...Bio care sent four of their people to California meeting workshop which by the way Invokes was invited by the society to give that workshop. Z.Naser President Innovex Biosciences James Watson wrote: #yiv617405017 v\:* {}#yiv617405017 o\:* {}#yiv617405017 w\:* {}#yiv617405017 .shape {} By saying that I do not know this company I meant that I do not know the people in you company.. I do order your products and have your website saved as a favorite on my computer. I have also received your flyers about your workshop and they do deal with your histo-stat, and stat-Q systems. I do not object to companies giving workshops, I have been involved with getting David Tacha of biocare medical and other companies to give seminars at the San Diego chapter of the CSH. I just think that the Histonet is not for vender use. Obviously I am not the only one that feels this way. I actually attended this workshop when it was given at a Tri-State meeting last spring, and I agree with James' remarks below. Since that time I've received dozens of workshop notices from Innovex, both directly and through the Histonet, advertising subsequent dates and places. I wrote to Innovex, asking to be taken off their email list (which they promptly did). I also wrote to Histonet about it at that time as well, asking them to look into the appropriateness of advertising Innovex's workshops here on our site, but I see that the workshop notices are still being forwarded to all members. I don't mind being approached individually by companies, but I really don't think that the Histonet should be used in this manner. --------------------------------- From: INNOVEX BIOSCIENCES [mailto:innvx@sbcglobal.net] Sent: Monday, August 20, 2007 2:07 PM To: James Watson Subject: RE: [Histonet] Animal IHC workshop at Duke university This is an educational From Maria.Mejia <@t> ucsf.edu Mon Aug 20 19:05:55 2007 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Mon Aug 20 19:07:02 2007 Subject: [Histonet] polymer protocols for 40um free-floating sections Message-ID: <6CF686BD6F24A546B85B24FE3B97864701B78F61@EXVS06.net.ucsf.edu> Hello, Please if anyone in histoland has done polymer staining on aldehyde fixed 40um free- floating primate or rat sections - if you could kindly share your standard protocols or provide suggestions or tips to stain these type of sections. I'm particularly interested in the anti-rabbit Iba1 using rabbit HRP polymer kit & anti-goat hNeurturin (NTN) using goat HRP polymer. I look forward to hearing from someone. Best Regards Maria Bartola Mejia UCSF Department of Neurosurgery SF CA 94103 From jqb7 <@t> CDC.GOV Tue Aug 21 04:46:00 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Aug 21 04:46:18 2007 Subject: [Histonet] Animal IHC workshop at Duke university In-Reply-To: <000601c7e36b$2af65120$3601a8c0@brownpathology.net> References: <000601c7e36b$2af65120$3601a8c0@brownpathology.net> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBF6E@LTA3VS003.ees.hhs.gov> I agree. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Monday, August 20, 2007 4:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university That's what the delete button is for.... if I were doing much animal tissue, I'd like to know when a free workshop with CEU's was close by.... regardless of commercial content. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Monday, August 20, 2007 3:29 PM To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university I do not know this company, but I do believe that this is blatant advertising of your products under the false pretense of an animal IHC staining workshop. I did not think that the Histonet was to be used by companies for advertising. I do not see other companies that give CEU approved courses advertising on the Histonet, why should Innovex be allowed to. Just my opinion on a busy Monday. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX BIOSCIENCES Sent: Monday, August 20, 2007 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal IHC workshop at Duke university Animal IHC staining workshop, Duke university, September 11, 9-12 ( morning session), CEU approved., a few spaces still available. This is an educational workshop that targets performing animal IHC staining and Mouse-on- Mouse IHC staining without background and under 2 hours. Register on line at innvx.com or call 1-800-622-7808. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Tue Aug 21 05:28:25 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Aug 21 05:28:44 2007 Subject: [Histonet] Molecular sieves for anhydrous acetone Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40FFA0EFE@ISS-CL-EX-V1.soton.ac.uk> Hi All, I realise this has been discussed before but just another quick question........... I want to use some molecular sieves (3A) to keep my acetone anhydrous (I will use the acetone for fixing frozen tissue sections). How much should I use and how long do they last? I got them from Sigma but there was no protocol except to say that 100g should hold 21g water. My acetone will only have a very small amount of water so if I put a lot of sieves in (say 10-20g in 100ml) how long will it last ie. days, weeks, months, years before I need to change the sieves? Also, someone mentioned before that they use dialysis tubing to keep the sieves in so that they don't get the sediment in the solution - but doesn't dialysis tubing need to be soaked in water before use? Thanks for your help guys! Sonya From louise.renton <@t> gmail.com Tue Aug 21 06:28:23 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Aug 21 06:28:28 2007 Subject: [Histonet] $40/day subsistence- slightly OT Message-ID: Dear USA Histonetters, My colleague & I will be attending the NSH congress in Denver later this year (hopefully), and are being offered $40.00 per day as subsistence to cover meals, taxis etc. In your collective expert opinion, is this enough to get a daily snacks, coffee and a decent meal now & then? I have no desire to exist on a constant diet of big macs & french fries - at my age a women has to watch her waist ( or whats left of it!) best regards & a big smile -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From pmarcum <@t> vet.upenn.edu Tue Aug 21 07:35:53 2007 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Tue Aug 21 07:36:02 2007 Subject: [Histonet] Animal IHC workshop at Duke university In-Reply-To: <34BB307EFC9A65429BBB49E330675F7202BDBF6E@LTA3VS003.ees.hhs.gov> References: <000601c7e36b$2af65120$3601a8c0@brownpathology.net> <34BB307EFC9A65429BBB49E330675F7202BDBF6E@LTA3VS003.ees.hhs.gov> Message-ID: <1187699753.46cadc291ae0f@imp.vet.upenn.edu> I was not going to make a comment as I know Jamie and this is one time I must disagree with him. We have invited Innovex to speak at the Region II meeting in Delaware and have had great response to the talk. I am one of the people doing animal IHC and I will admit I need help with some of the areas no one addresses with large animals. No One in the clincal companies offer enough help for this area so I for one can hardly wait to go to this workshop and get more information. I can always tailor it to my needs or know more about what to do with some issues. I will still be free to buy from whomever I wish as the class does not commit or any other attedee to the company itself. I hope they start to annouce here soon and I know it will be in my post to HistoNet about the meeting. Pam Marcum Quoting "Bartlett, Jeanine (CDC/CCID/NCZVED)" : > I agree. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie > Whitaker > Sent: Monday, August 20, 2007 4:46 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > That's what the delete button is for.... if I were doing much animal > tissue, I'd like to know when a free workshop with CEU's was close > by.... regardless of commercial content. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James > Watson > Sent: Monday, August 20, 2007 3:29 PM > To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > > I do not know this company, but I do believe that this is blatant > advertising of your products under the false pretense of an animal IHC > staining workshop. I did not think that the Histonet was to be used by > companies for advertising. I do not see other companies that give CEU > approved courses advertising on the Histonet, why should Innovex be > allowed to. > > Just my opinion on a busy Monday. > > James Watson HT ASCP > Facilities Manager of Histology > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX > BIOSCIENCES > Sent: Monday, August 20, 2007 1:04 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Animal IHC workshop at Duke university > > Animal IHC staining workshop, Duke university, September 11, 9-12 ( > morning session), CEU approved., a few spaces still available. This is > an educational workshop that targets performing animal IHC staining and > Mouse-on- Mouse IHC staining without background and under 2 hours. > Register on line at innvx.com or call 1-800-622-7808. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ree3 <@t> leicester.ac.uk Tue Aug 21 08:04:36 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Aug 21 08:04:42 2007 Subject: [Histonet] $40/day subsistence- slightly OT In-Reply-To: References: Message-ID: $40 a day on burgers, sounds like heaven to me, just had a Macdons burger and chips for my lunch. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 21 August 2007 12:28 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] $40/day subsistence- slightly OT Dear USA Histonetters, My colleague & I will be attending the NSH congress in Denver later this year (hopefully), and are being offered $40.00 per day as subsistence to cover meals, taxis etc. In your collective expert opinion, is this enough to get a daily snacks, coffee and a decent meal now & then? I have no desire to exist on a constant diet of big macs & french fries - at my age a women has to watch her waist ( or whats left of it!) best regards & a big smile -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Tue Aug 21 08:13:34 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Aug 21 08:13:46 2007 Subject: [Histonet] IF staining of mast cells and fibroblasts Message-ID: <310809.3031.qm@web50311.mail.re2.yahoo.com> Thanks to everyone who responded. Once again, the histonet proves to be an invaluable resource! Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Bernice Frederick To: Liz Chlipala ; Kim Merriam ; Histonet Sent: Monday, August 20, 2007 1:59:01 PM Subject: RE: [Histonet] IF staining of mast cells and fibroblasts Mast cell tryptase works fine on human 1:8000. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Monday, August 20, 2007 11:20 AM To: Kim Merriam; Histonet Subject: RE: [Histonet] IF staining of mast cells and fibroblasts Kim Mast cell tryptase works in mice also, so I suspect it might work in other species. For fibroblasts there is a marker that works in rat its mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody 6-9H6 (AF5110-1) from Acris. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, August 20, 2007 9:46 AM To: Histonet Subject: [Histonet] IF staining of mast cells and fibroblasts Hello all, I was wondering if anyone knew of specific markers for mast cells and fibroblasts. I need markers for IF and IHC (not special stains) that will stain only mast cells and only fibroblasts. We have a few markers in mind, but we are not sure if they are specific only to those types of cells and will not cross-react with some other cell type. Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ________________________________________________________________________ ____________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+ki ds&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: 8/19/2007 7:27 AM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date: 8/19/2007 7:27 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. http://mobile.yahoo.com/go?refer=1GNXIC From pruegg <@t> ihctech.net Tue Aug 21 08:24:33 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Aug 21 08:21:35 2007 Subject: [Histonet] Animal IHC workshop at Duke university In-Reply-To: <1187699753.46cadc291ae0f@imp.vet.upenn.edu> Message-ID: <200708211321.l7LDLIRf014281@pro12.abac.com> Are you kidding me. We have come a long way from barring the vendors from doing ws at the NSH S/C or anywhere else. Without the support of our vendors (who by the way have some very knowledgeable speakers many of whom came from histology) the national and local conferences would not survive. In the past 10 years I have gotten as much or more from vendor sponsored courses as I have gotten from non-vendor courses. I say let them advertise all they want to offer FREE educational courses. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pmarcum@vet.upenn.edu Sent: Tuesday, August 21, 2007 6:36 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university I was not going to make a comment as I know Jamie and this is one time I must disagree with him. We have invited Innovex to speak at the Region II meeting in Delaware and have had great response to the talk. I am one of the people doing animal IHC and I will admit I need help with some of the areas no one addresses with large animals. No One in the clincal companies offer enough help for this area so I for one can hardly wait to go to this workshop and get more information. I can always tailor it to my needs or know more about what to do with some issues. I will still be free to buy from whomever I wish as the class does not commit or any other attedee to the company itself. I hope they start to annouce here soon and I know it will be in my post to HistoNet about the meeting. Pam Marcum Quoting "Bartlett, Jeanine (CDC/CCID/NCZVED)" : > I agree. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie > Whitaker > Sent: Monday, August 20, 2007 4:46 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > That's what the delete button is for.... if I were doing much animal > tissue, I'd like to know when a free workshop with CEU's was close > by.... regardless of commercial content. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James > Watson > Sent: Monday, August 20, 2007 3:29 PM > To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > > I do not know this company, but I do believe that this is blatant > advertising of your products under the false pretense of an animal IHC > staining workshop. I did not think that the Histonet was to be used by > companies for advertising. I do not see other companies that give CEU > approved courses advertising on the Histonet, why should Innovex be > allowed to. > > Just my opinion on a busy Monday. > > James Watson HT ASCP > Facilities Manager of Histology > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX > BIOSCIENCES > Sent: Monday, August 20, 2007 1:04 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Animal IHC workshop at Duke university > > Animal IHC staining workshop, Duke university, September 11, 9-12 ( > morning session), CEU approved., a few spaces still available. This is > an educational workshop that targets performing animal IHC staining and > Mouse-on- Mouse IHC staining without background and under 2 hours. > Register on line at innvx.com or call 1-800-622-7808. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Aug 21 08:57:44 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Aug 21 08:59:07 2007 Subject: AW: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <000c01c7e1b9$fb9eb4b0$0202fea9@dielangs.at> References: <000c01c7e1b9$fb9eb4b0$0202fea9@dielangs.at> Message-ID: <46CAEF58.3080300@umdnj.edu> Dear Gudrun: Try a public library, university library or second-hand book store for old histology texts. Also see if you can find a copy of "Cytological Technique" or "Principles of Biological Microtechnique" both by John R. Baker. Classic works in the field. Geoff Gudrun Lang wrote: > Geoff and Bryan, > Unfortunatly I have no access to these books. But I hoped someone could > describe in a few words the essential differences between formalin and > formalin-fixed tissue. > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] > Gesendet: Freitag, 17. August 2007 20:46 > An: gu.lang@gmx.at > Cc: histonet@lists.utsouthwestern.edu > Betreff: Re: [Histonet] alcohol fixation image (2nd try) > > I recall that an early edition (1960's or so) of one of the major > histology texts (Bloom and Fawcett's, A Textbook of Histology I think) > had drawing of how and intestinal epithelial cell would look after > various fixatives. Also try some older editions (pre 1960's) of Maximow > and Bloom, Bailey, Ham etc, all of the major histo texts that are too > long and encyclopedic for today's medical student. > > Geoff > > > Gudrun Lang wrote: > >> Hi, >> >> I have to admit, that I need help with basic histotechnical knowledge. >> Please, can someone describe me the "alcohol fixation image" of tissue, >> especially chromatin? >> >> And would poorly formalin-fixed tissue show less chromatin-structure or >> > more > >> intense chromatin-structure, after the usual VIP-processing. >> (50-70-96-96-100-100 alc.) due to the ethanol-effect? >> >> >> >> Thanks in advance >> >> Gudrun Lang >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From slappycraw <@t> yahoo.com Tue Aug 21 09:12:38 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Aug 21 09:12:42 2007 Subject: [Histonet] $40/day subsistence- slightly OT In-Reply-To: Message-ID: <701500.34111.qm@web53604.mail.re2.yahoo.com> Rachael Ray does it all the time "Edwards, R.E." wrote: $40 a day on burgers, sounds like heaven to me, just had a Macdons burger and chips for my lunch. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 21 August 2007 12:28 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] $40/day subsistence- slightly OT Dear USA Histonetters, My colleague & I will be attending the NSH congress in Denver later this year (hopefully), and are being offered $40.00 per day as subsistence to cover meals, taxis etc. In your collective expert opinion, is this enough to get a daily snacks, coffee and a decent meal now & then? I have no desire to exist on a constant diet of big macs & french fries - at my age a women has to watch her waist ( or whats left of it!) best regards & a big smile -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From jnocito <@t> satx.rr.com Tue Aug 21 09:16:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 21 09:18:24 2007 Subject: [Histonet] $40/day subsistence- slightly OT References: Message-ID: <000e01c7e3fd$e7843220$d49eae18@yourxhtr8hvc4p> I've never had a problem, then again, that's when bargaining with vendors comes in. There, I've said it. I'll do anything for a steak and potato. JTT ----- Original Message ----- From: "Edwards, R.E." To: "louise renton" ; Sent: Tuesday, August 21, 2007 8:04 AM Subject: RE: [Histonet] $40/day subsistence- slightly OT $40 a day on burgers, sounds like heaven to me, just had a Macdons burger and chips for my lunch. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 21 August 2007 12:28 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] $40/day subsistence- slightly OT Dear USA Histonetters, My colleague & I will be attending the NSH congress in Denver later this year (hopefully), and are being offered $40.00 per day as subsistence to cover meals, taxis etc. In your collective expert opinion, is this enough to get a daily snacks, coffee and a decent meal now & then? I have no desire to exist on a constant diet of big macs & french fries - at my age a women has to watch her waist ( or whats left of it!) best regards & a big smile -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garth <@t> apollosci.co.za Tue Aug 21 09:16:23 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Tue Aug 21 09:18:28 2007 Subject: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <000d01c7e1bb$3af076c0$0202fea9@dielangs.at> Message-ID: <002801c7e3fd$df1561e0$7700a8c0@jhb.apollosci.co.za> Hello Gudrun We recently ran an assay in conjunction with a local pathology laboratory, to compare in detail, the difference cell morphology of formalin and alchohol fixed / processed tissue. We are in the process of taking digital images of the slides and will be uploading these to a website. If you like, when done, I can try to arrange to send you a few. Please advise if you would like me to do so. Garth Jerome Histologist Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 18 August 2007 07:14 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] alcohol fixation image (2nd try) Uups, should be "formalin and alcohol-fixed". Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Samstag, 18. August 2007 19:05 An: 'Geoff McAuliffe' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] alcohol fixation image (2nd try) Geoff and Bryan, Unfortunatly I have no access to these books. But I hoped someone could describe in a few words the essential differences between formalin and formalin-fixed tissue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Freitag, 17. August 2007 20:46 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] alcohol fixation image (2nd try) I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Aug 21 10:34:19 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Aug 21 09:35:43 2007 Subject: {SPAM?} [Histonet] $40/day subsistence- slightly OT In-Reply-To: Message-ID: Just make sure you can find a place that has cheap hot wings and dollar drafts. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Tuesday, August 21, 2007 6:28 AM To: Histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] $40/day subsistence- slightly OT Dear USA Histonetters, My colleague & I will be attending the NSH congress in Denver later this year (hopefully), and are being offered $40.00 per day as subsistence to cover meals, taxis etc. In your collective expert opinion, is this enough to get a daily snacks, coffee and a decent meal now & then? I have no desire to exist on a constant diet of big macs & french fries - at my age a women has to watch her waist ( or whats left of it!) best regards & a big smile -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Tue Aug 21 09:57:34 2007 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Tue Aug 21 09:57:16 2007 Subject: [Histonet] MARS probe Message-ID: <7DFAF4868AAAC34C986DF7E1AC16D0266E7EBE@pgnexchg1.pathgroup.com> Hello fellow micowavers (very small wave). Is anyone having trouble w/ the temperature probe on the MARS microwave tissue processor by Hacker? I have a wonderful new unit that was working really well, until we hit a small paraffin plug in the lid and damaged (ruined) the probe. I'm still reeling from the replacement price to put the unit back in service. Just wanted to know if anyone else is having issues? Peace & Thank you Bruce Gapinski --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From pruegg <@t> ihctech.net Tue Aug 21 10:14:48 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Aug 21 10:11:57 2007 Subject: [Histonet] alcohol fixation image (2nd try) In-Reply-To: <002801c7e3fd$df1561e0$7700a8c0@jhb.apollosci.co.za> Message-ID: <200708211511.l7LFBXuQ074449@pro12.abac.com> I think an important issue with alcohol fixation vs formalin is with IHC differences, Gudrun did you compare IHC protocols on the formalin fixed vs alcohol fixed samples? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garth Jerome Sent: Tuesday, August 21, 2007 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] alcohol fixation image (2nd try) Hello Gudrun We recently ran an assay in conjunction with a local pathology laboratory, to compare in detail, the difference cell morphology of formalin and alchohol fixed / processed tissue. We are in the process of taking digital images of the slides and will be uploading these to a website. If you like, when done, I can try to arrange to send you a few. Please advise if you would like me to do so. Garth Jerome Histologist Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 18 August 2007 07:14 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] alcohol fixation image (2nd try) Uups, should be "formalin and alcohol-fixed". Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Samstag, 18. August 2007 19:05 An: 'Geoff McAuliffe' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] alcohol fixation image (2nd try) Geoff and Bryan, Unfortunatly I have no access to these books. But I hoped someone could describe in a few words the essential differences between formalin and formalin-fixed tissue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Freitag, 17. August 2007 20:46 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] alcohol fixation image (2nd try) I recall that an early edition (1960's or so) of one of the major histology texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of how and intestinal epithelial cell would look after various fixatives. Also try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, all of the major histo texts that are too long and encyclopedic for today's medical student. Geoff Gudrun Lang wrote: > Hi, > > I have to admit, that I need help with basic histotechnical knowledge. > Please, can someone describe me the "alcohol fixation image" of tissue, > especially chromatin? > > And would poorly formalin-fixed tissue show less chromatin-structure or more > intense chromatin-structure, after the usual VIP-processing. > (50-70-96-96-100-100 alc.) due to the ethanol-effect? > > > > Thanks in advance > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Tue Aug 21 10:26:09 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Aug 21 10:29:32 2007 Subject: [Histonet] Herlant Pituitary Stain II Message-ID: <6.2.3.4.1.20070821081220.01edb458@algranth.inbox.email.arizona.edu> Good Morning! I am getting ready to do a Herlant Stain on pituitary and I am confused by the protocol in Humason's Animal Techniques, 3rd edition. If there is anybody out in histoland who is familiar with this stain OR if you have this book and can help with interpretation of the protocol please let me hear from you. Thanks, Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From jwatson <@t> gnf.org Tue Aug 21 11:06:58 2007 From: jwatson <@t> gnf.org (James Watson) Date: Tue Aug 21 11:07:07 2007 Subject: FW: [Histonet] Animal IHC workshop at Duke university References: <200708211321.l7LDLIRf014281@pro12.abac.com> Message-ID: I apologize for not stating my opinion clearly enough yesterday (it was Monday morning). I am not commenting on vendors giving courses. We would loose a lot of training if vendors did not give classes. I have helped arrange and advertised on the histonet vender taught classes myself. I did not say anything when a histo tech forwarded their advertisement about the course. I was not attacking this company. When I said I did not know this company I meant to say I do not know anyone from this company. I do use some of their products; their FC receptor block has worked extremely well on skin samples when staining for c-kit and phospho-c-kit in mast cells. My opinion is that vendors should not be able to post on the Histonet directly. I guess we have set a new precedent, vendors are allowed direct access to the Histonet, so Dako, Biocare, Ventana, Leica, and all the other vendors that offer courses should be allowed direct access to the Histonet. I will return to being a silent observer on the Histonet and only responding directly to individuals about questions. And yes I do know what the delete button is. Thank you. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of patsy ruegg Sent: Tue 8/21/2007 6:24 AM To: pmarcum@vet.upenn.edu; 'Bartlett, Jeanine (CDC/CCID/NCZVED)' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university Are you kidding me. We have come a long way from barring the vendors from doing ws at the NSH S/C or anywhere else. Without the support of our vendors (who by the way have some very knowledgeable speakers many of whom came from histology) the national and local conferences would not survive. In the past 10 years I have gotten as much or more from vendor sponsored courses as I have gotten from non-vendor courses. I say let them advertise all they want to offer FREE educational courses. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pmarcum@vet.upenn.edu Sent: Tuesday, August 21, 2007 6:36 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university I was not going to make a comment as I know Jamie and this is one time I must disagree with him. We have invited Innovex to speak at the Region II meeting in Delaware and have had great response to the talk. I am one of the people doing animal IHC and I will admit I need help with some of the areas no one addresses with large animals. No One in the clincal companies offer enough help for this area so I for one can hardly wait to go to this workshop and get more information. I can always tailor it to my needs or know more about what to do with some issues. I will still be free to buy from whomever I wish as the class does not commit or any other attedee to the company itself. I hope they start to annouce here soon and I know it will be in my post to HistoNet about the meeting. Pam Marcum Quoting "Bartlett, Jeanine (CDC/CCID/NCZVED)" : > I agree. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie > Whitaker > Sent: Monday, August 20, 2007 4:46 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > That's what the delete button is for.... if I were doing much animal > tissue, I'd like to know when a free workshop with CEU's was close > by.... regardless of commercial content. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James > Watson > Sent: Monday, August 20, 2007 3:29 PM > To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > > I do not know this company, but I do believe that this is blatant > advertising of your products under the false pretense of an animal IHC > staining workshop. I did not think that the Histonet was to be used by > companies for advertising. I do not see other companies that give CEU > approved courses advertising on the Histonet, why should Innovex be > allowed to. > > Just my opinion on a busy Monday. > > James Watson HT ASCP > Facilities Manager of Histology > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX > BIOSCIENCES > Sent: Monday, August 20, 2007 1:04 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Animal IHC workshop at Duke university > > Animal IHC staining workshop, Duke university, September 11, 9-12 ( > morning session), CEU approved., a few spaces still available. This is > an educational workshop that targets performing animal IHC staining and > Mouse-on- Mouse IHC staining without background and under 2 hours. > Register on line at innvx.com or call 1-800-622-7808. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Aug 21 11:51:31 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 21 11:51:37 2007 Subject: [Histonet] Animal IHC workshop at Duke university References: <200708211321.l7LDLIRf014281@pro12.abac.com> Message-ID: <002701c7e413$89245640$d49eae18@yourxhtr8hvc4p> James, welcome to the world of being flamed. But please don't stop your opinions because of a few people. If everyone thought alike, this would be a dull world. I thought as you did once and was going to remain silent. Even after the threat of loosing my job, I still gave my opinions. I even left for a while, but didn't feel right. I guess I just have too big of mouth to be kept silent. Welcome to the flamed club, but please be vocal about you opinions. Been there, done that JTT ----- Original Message ----- From: "James Watson" To: Sent: Tuesday, August 21, 2007 11:06 AM Subject: FW: [Histonet] Animal IHC workshop at Duke university I apologize for not stating my opinion clearly enough yesterday (it was Monday morning). I am not commenting on vendors giving courses. We would loose a lot of training if vendors did not give classes. I have helped arrange and advertised on the histonet vender taught classes myself. I did not say anything when a histo tech forwarded their advertisement about the course. I was not attacking this company. When I said I did not know this company I meant to say I do not know anyone from this company. I do use some of their products; their FC receptor block has worked extremely well on skin samples when staining for c-kit and phospho-c-kit in mast cells. My opinion is that vendors should not be able to post on the Histonet directly. I guess we have set a new precedent, vendors are allowed direct access to the Histonet, so Dako, Biocare, Ventana, Leica, and all the other vendors that offer courses should be allowed direct access to the Histonet. I will return to being a silent observer on the Histonet and only responding directly to individuals about questions. And yes I do know what the delete button is. Thank you. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of patsy ruegg Sent: Tue 8/21/2007 6:24 AM To: pmarcum@vet.upenn.edu; 'Bartlett, Jeanine (CDC/CCID/NCZVED)' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university Are you kidding me. We have come a long way from barring the vendors from doing ws at the NSH S/C or anywhere else. Without the support of our vendors (who by the way have some very knowledgeable speakers many of whom came from histology) the national and local conferences would not survive. In the past 10 years I have gotten as much or more from vendor sponsored courses as I have gotten from non-vendor courses. I say let them advertise all they want to offer FREE educational courses. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pmarcum@vet.upenn.edu Sent: Tuesday, August 21, 2007 6:36 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university I was not going to make a comment as I know Jamie and this is one time I must disagree with him. We have invited Innovex to speak at the Region II meeting in Delaware and have had great response to the talk. I am one of the people doing animal IHC and I will admit I need help with some of the areas no one addresses with large animals. No One in the clincal companies offer enough help for this area so I for one can hardly wait to go to this workshop and get more information. I can always tailor it to my needs or know more about what to do with some issues. I will still be free to buy from whomever I wish as the class does not commit or any other attedee to the company itself. I hope they start to annouce here soon and I know it will be in my post to HistoNet about the meeting. Pam Marcum Quoting "Bartlett, Jeanine (CDC/CCID/NCZVED)" : > I agree. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie > Whitaker > Sent: Monday, August 20, 2007 4:46 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > That's what the delete button is for.... if I were doing much animal > tissue, I'd like to know when a free workshop with CEU's was close > by.... regardless of commercial content. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James > Watson > Sent: Monday, August 20, 2007 3:29 PM > To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > > I do not know this company, but I do believe that this is blatant > advertising of your products under the false pretense of an animal IHC > staining workshop. I did not think that the Histonet was to be used by > companies for advertising. I do not see other companies that give CEU > approved courses advertising on the Histonet, why should Innovex be > allowed to. > > Just my opinion on a busy Monday. > > James Watson HT ASCP > Facilities Manager of Histology > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX > BIOSCIENCES > Sent: Monday, August 20, 2007 1:04 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Animal IHC workshop at Duke university > > Animal IHC staining workshop, Duke university, September 11, 9-12 ( > morning session), CEU approved., a few spaces still available. This is > an educational workshop that targets performing animal IHC staining and > Mouse-on- Mouse IHC staining without background and under 2 hours. > Register on line at innvx.com or call 1-800-622-7808. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Aug 21 11:52:46 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 21 11:55:46 2007 Subject: {SPAM?} [Histonet] $40/day subsistence- slightly OT References: <5glv84$4ed50s@orngca-mx-03.mgw.rr.com> Message-ID: <003e01c7e413$b3d0b6e0$d49eae18@yourxhtr8hvc4p> hope Denver is like that. In Columbus, it was $40 just to get a cab to Denny's. JTT ----- Original Message ----- From: "Douglas D Deltour" To: "'louise renton'" ; Sent: Tuesday, August 21, 2007 10:34 AM Subject: RE: {SPAM?} [Histonet] $40/day subsistence- slightly OT > Just make sure you can find a place that has cheap hot wings and dollar > drafts. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Tuesday, August 21, 2007 6:28 AM > To: Histonet@lists.utsouthwestern.edu > Subject: {SPAM?} [Histonet] $40/day subsistence- slightly OT > > Dear USA Histonetters, > > My colleague & I will be attending the NSH congress in Denver later this > year (hopefully), and are being offered $40.00 per day as subsistence to > cover meals, taxis etc. In your collective expert opinion, is this enough > to > get a daily snacks, coffee and a decent meal now & then? I have no desire > to > exist on a constant diet of big macs & french fries - at my age a women > has > to watch her waist ( or whats left of it!) > > best regards & a big smile > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Aug 21 12:04:49 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Aug 21 12:05:05 2007 Subject: [Histonet] prostate needle biopsies In-Reply-To: <000601c7e059$f8a40b40$09650744@pereirafarm> Message-ID: <002401c7e415$624ba800$1d2a14ac@wchsys.org> We use the screened cassettes, this means no tissue wrapping, no sponge sticking problems. The prostate needle cores can be removed without breaking and can be embedded in full length. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pereirafamily Sent: Thursday, August 16, 2007 7:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate needle biopsies Is anyone using anything other than sponges in their cassettes for prostate needle biopsies? If so, do you find that it works better? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From jkiernan <@t> uwo.ca Tue Aug 21 12:07:23 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Aug 21 12:07:29 2007 Subject: [Histonet] Herlant Pituitary Stain II In-Reply-To: <6.2.3.4.1.20070821081220.01edb458@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20070821081220.01edb458@algranth.inbox.email.arizona.edu> Message-ID: This method is a simplification of Adams's pituitary stain, which is the sequence: Performic or peracetic acid - oxidizes cystine -S-S- to cysteic acid, a sulphonic (strong) acid. Alcian blue pH 0.2 - stains the generated sulphonic acid groups (TSH cells and neurosecretory material of the posterior lobe). PAS - stains neutral glycoproteins, especially cells producing gonadotrophic hormones Orange G in PTA - stains growth hormone cells. Mayer's Haemalum - for nuclei. I've not read Herlant's 1960 paper, the one cited in Humason's Animal tissue Techniques, but have read a related one: Pasteels & Herlant 1962 Z. Zellforsch. 56: 20-39. The principal innovation was replacing performic or peracetic acid with acidified potassium permanganate followed by a bisulphite rinse to remove brown MnO2 from the sections. You can use 1% oxalic acid instead of bisulphite. I've done these methods and recommend permanganate over the organic per-acids, which are really noxious. Some histochemists have argued against permanganate oxidation (see books by A.G.E.Pearse and by M.Gabe) but none can deny that permanganate-H2SO4 followed by alcian blue pH 0.2 gives good staining of neurosecretory material. Mast cells are also blue because of their heparin content, but that staining does not require the prior oxidation. In the late 1950s rational techniques of this kind replaced earlier staining techniques for pituitary cell-types, which were mostly variants of Heidenhain's AZAN and other trichromes. Since the 1970s antibodies to all the anterior and posterior pituitary hormones have been available and the cell types can be unequivocally identified by immunohistochemistry. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Andrea Grantham Date: Tuesday, August 21, 2007 11:31 Subject: [Histonet] Herlant Pituitary Stain II To: histonet@lists.utsouthwestern.edu > Good Morning! > I am getting ready to do a Herlant Stain on pituitary and I am > confused by the protocol in Humason's Animal Techniques, 3rd edition. > > If there is anybody out in histoland who is familiar with this > stain > OR if you have this book and can help with interpretation of the > protocol please let me hear from you. > > Thanks, > > Andi Grantham > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of > Cell Biology & Anatomy : > : Sr. Research Specialist > University of > Arizona : > : (office: AHSC > 4212) P.O. > Box 245044 : > : (voice: 520-626- > 4415) Tucson, AZ > 85724-5044 > USA : > : (FAX: 520-626- > 2097) (email: algranth@u.arizona.edu) : > :...................................................................: > > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbreeden <@t> nmda.nmsu.edu Tue Aug 21 12:15:52 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Aug 21 12:15:58 2007 Subject: [Histonet] Help - Nuclear Fast Red Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4637@nmdamailsvr.nmda.ad.nmsu.edu> I'm in the middle of a Von Kossa and my Nuclear Fast Red is missing! What can I use as a substitute? Help! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Dorothy.L.Webb <@t> HealthPartners.Com Tue Aug 21 12:19:19 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Aug 21 12:19:25 2007 Subject: [Histonet] HELP Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056350C0@hpes1.HealthPartners.int> What would cause the tissues when taken out of the processor at the end of the cycle to feel "slippery", almost greasy? I was off and back today and when the tech took the cassettes out of the processor, she mentioned this and they are not cutting well at all. Our processor was just changed yesterday and as far as I can tell from smell, all reagents are in order! Where should I start????? Thanks so much for answering this so quickly..I am stymied!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From MVaughan4 <@t> ucok.edu Tue Aug 21 12:23:16 2007 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Tue Aug 21 12:23:32 2007 Subject: [Histonet] Myofibroblast vs fibroblast In-Reply-To: Message-ID: Igor, Use mouse anti-human alpha-smooth muscle actin, clone IA4, available from Sigma, Labvision, and probably everybody else. Works at 1:1000 for immunofluorescence and also works for IHC. It is currently the one and only marker to differentiate the two cell types from each other. I used the Sigma antibody in this publication: Vaughan, M.B., Howard, E.W., and Tomasek, J.J. (2000). Transforming growth factor-?1 promotes the morphological and functional differentiation of the myofibroblast. Exp. Cell Res. 257:180-189. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From PMonfils <@t> Lifespan.org Tue Aug 21 12:26:24 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Aug 21 12:26:30 2007 Subject: [Histonet] Help - Nuclear Fast Red In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4637@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CCD@LSRIEXCH1.lsmaster.lifespan.org> Safranin works well. In fact I often use it with Von Kossa in order to demonstrate both calcium and cartilage, followed by Fast Green. From PMonfils <@t> Lifespan.org Tue Aug 21 12:28:46 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Aug 21 12:28:51 2007 Subject: [Histonet] HELP In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056350C0@hpes1.HealthPartners.int> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CCE@LSRIEXCH1.lsmaster.lifespan.org> I would suspect water. Do you have an alcohol hydrometer, to make sure that your absolute alcohols really are absolute? From king.laurie <@t> marshfieldclinic.org Tue Aug 21 12:32:55 2007 From: king.laurie <@t> marshfieldclinic.org (king.laurie@marshfieldclinic.org) Date: Tue Aug 21 12:33:00 2007 Subject: [Histonet] HELP Message-ID: Sounds like xylene in the paraffin to me. Laurie ------Original Message------ From: "Monfils, Paul" Date: Tue Aug 21, 2007 -- 12:30:59 PM To: "histonet@lists.utsouthwestern.edu" Subject: RE: [Histonet] HELP I would suspect water. Do you have an alcohol hydrometer, to make sure that your absolute alcohols really are absolute? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Aug 21 12:36:13 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 21 12:36:26 2007 Subject: [Histonet] HELP In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056350C0@hpes1.HealthPartners.int> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6C2@IS-E2K3.grhs.net> The amount of time you spend trying to figure out what happened you can change your processor again and then look at the problem after the fact. It is almost a given that something was misplaced/or wrong solution on the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, August 21, 2007 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP What would cause the tissues when taken out of the processor at the end of the cycle to feel "slippery", almost greasy? I was off and back today and when the tech took the cassettes out of the processor, she mentioned this and they are not cutting well at all. Our processor was just changed yesterday and as far as I can tell from smell, all reagents are in order! Where should I start????? Thanks so much for answering this so quickly..I am stymied!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Aug 21 13:36:31 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Aug 21 12:37:49 2007 Subject: [Histonet] HELP In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056350C0@hpes1.HealthPartners.int> Message-ID: Check the last paraffin. Does it smell like Xylene? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, August 21, 2007 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP What would cause the tissues when taken out of the processor at the end of the cycle to feel "slippery", almost greasy? I was off and back today and when the tech took the cassettes out of the processor, she mentioned this and they are not cutting well at all. Our processor was just changed yesterday and as far as I can tell from smell, all reagents are in order! Where should I start????? Thanks so much for answering this so quickly..I am stymied!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Aug 21 12:47:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 21 12:47:10 2007 Subject: [Histonet] Help - Nuclear Fast Red In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4637@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <284988.41113.qm@web61220.mail.yahoo.com> Use 1% aq. acid fuchsin. Ren? J. "Breeden, Sara" wrote: I'm in the middle of a Von Kossa and my Nuclear Fast Red is missing! What can I use as a substitute? Help! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From Shirley_PHUA <@t> hsa.gov.sg Tue Aug 21 13:01:33 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue Aug 21 13:05:17 2007 Subject: [Histonet] Shirley Phua is away on 21 Aug 2007 (Tuesday) afternoon. Message-ID: I will be out of the office from 21-08-2007 to 22-08-2007. I'll be away on 21 Aug 2007 (Tuesday). I'll be back on 22 August 2007 (Wednesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From integrated.histo <@t> gmail.com Tue Aug 21 13:19:08 2007 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Tue Aug 21 13:19:16 2007 Subject: [Histonet] Breast processing Message-ID: <5d9104a30708211119m53aa2f95v2977413e35ca2857@mail.gmail.com> We have two processors, one for biopsies, tonsils, appendix and the like; and the other for breast, colon, placenta, etc. I love the results we get. Cindy DuBois Integrated Pathology From jqb7 <@t> CDC.GOV Tue Aug 21 12:58:53 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Aug 21 13:39:01 2007 Subject: [Histonet] microwaves in labs Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From tkngflght <@t> yahoo.com Tue Aug 21 15:44:11 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Aug 21 15:44:15 2007 Subject: [Histonet] Histo temp and permanent jobs available now! Message-ID: <362606.16035.qm@web50906.mail.re2.yahoo.com> Hi All-- We have a new little bunch of temp jobs--three to fill by next week. Give me a call if you want to share a 'Histotemp 101' conversation. If you're an experienced traveler we can cut to the details of the jobs to see if any fit you! We have a slew of permanent positions--from Coordinator through new grad levels--all shifts and all over the country. The states in which we have the most openings in include: California Florida Georgia Massachusetts Maryland Missouri Nevada New York Ohio Texas Virginia and Washington. The conversation is fun and is no-risk for you. We're here to serve your family and your career--invest a five minutes and you'll get the difference. We offer support from resume creation through interview coaching, compensation negotiation and more. With direct histology experience and years in staffing, too, you get our knowledgeable assistance every step of the way. Give us a call ! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 800.756.3309 281.852.9457 admin@fullstaff.org From ekh9535 <@t> bjc.org Tue Aug 21 16:41:15 2007 From: ekh9535 <@t> bjc.org (Erin Herter) Date: Tue Aug 21 16:40:19 2007 Subject: [Histonet] Peloris versus Sakura X-press Message-ID: <46CB1555.9203.00A0.0@bjc.org> I would like to hear from anyone out there with either of or possibly both of these processors. We are especially interested in how it processes stereotactic breast biopsies. I would like to know how long it is taking to process these specimens AFTER the required 6 hour formalin fixation CAP requires for HER-2's. If you are using a different protocol than the recommended CAP, what are you doing and how did you do your validation study? Thank you very much in advance to anyone and everyone who can help me with this decision. Erin The secret of joy in work is contained in one word - excellence. To know how to do something well is to enjoy it. Pearl S. Buck Erin Herter, MLT (ASCP) Lead Technician, Histology Department Alton Memorial Hospital One Memorial Drive Alton, IL 62002 618-463-7454 Histology Lab 618-433-7082 My office 618-463-7641 fax ekh9535@bjc.org From rjbuesa <@t> yahoo.com Tue Aug 21 17:01:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 21 17:01:59 2007 Subject: [Histonet] Peloris versus Sakura X-press In-Reply-To: <46CB1555.9203.00A0.0@bjc.org> Message-ID: <589822.36723.qm@web61223.mail.yahoo.com> Erin: Just a word of caution: you are talking about "two different animals". Peloris is essentially a conventional tissue processor (TP) with some innovations, like the use of propanol as antemedium and its elimination by quickly heating the tissues off the propanol, before immersing them in paraffin. Protocols last approximately as any other conventional TP. It can process up to 600 cassettes in 9h, or 100 bx in 1.5 hours. X-Press is a mix of microwave (2 retorts) with conventional heating (2 additional retorts). In the first 2 dehydration and antemedium are treated with a low wattage microwave system. In the last 2 retorts infiltration takes place. The time the tissues (up to a maximum of 40 cassettes only) remain in each retort is 15 minutes, and you can add 40 cassettes to the first one every 15 minutes and get as many processed cassettes every 15 minutas after the first 45 minutes. Price is also for sure different, although I don't know the price of Peloris, the X-Press is sold for $250,000 and I don't think there is a single lab. using both. As you can see, they are "two different animals"> Ren? J. Erin Herter wrote: I would like to hear from anyone out there with either of or possibly both of these processors. We are especially interested in how it processes stereotactic breast biopsies. I would like to know how long it is taking to process these specimens AFTER the required 6 hour formalin fixation CAP requires for HER-2's. If you are using a different protocol than the recommended CAP, what are you doing and how did you do your validation study? Thank you very much in advance to anyone and everyone who can help me with this decision. Erin The secret of joy in work is contained in one word - excellence. To know how to do something well is to enjoy it. Pearl S. Buck Erin Herter, MLT (ASCP) Lead Technician, Histology Department Alton Memorial Hospital One Memorial Drive Alton, IL 62002 618-463-7454 Histology Lab 618-433-7082 My office 618-463-7641 fax ekh9535@bjc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From AnthonyH <@t> chw.edu.au Tue Aug 21 17:03:48 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 21 17:04:18 2007 Subject: [Histonet] Help - Nuclear Fast Red Message-ID: Neutral red will work quite well, or ethyl green. It is only a counterstain Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, 22 August 2007 3:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help - Nuclear Fast Red I'm in the middle of a Von Kossa and my Nuclear Fast Red is missing! What can I use as a substitute? Help! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mari.ann.mailhiot <@t> leica-microsystems.com Tue Aug 21 17:15:21 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Aug 21 17:15:05 2007 Subject: [Histonet] HELP In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056350C0@hpes1.HealthPartners.int> Message-ID: HI Dorothy It sounds like xylene is in the wax. The other possibility could be water. Check the bottom of the paraffin tank and see if you have droplets. If you do then it is more than likely water. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Webb, Dorothy L" histonet@lists.utsouthwestern.edu Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] HELP 08/21/2007 12:19 PM What would cause the tissues when taken out of the processor at the end of the cycle to feel "slippery", almost greasy? I was off and back today and when the tech took the cassettes out of the processor, she mentioned this and they are not cutting well at all. Our processor was just changed yesterday and as far as I can tell from smell, all reagents are in order! Where should I start????? Thanks so much for answering this so quickly..I am stymied!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Erin.Martin <@t> ucsf.edu Tue Aug 21 17:36:21 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Aug 21 17:38:32 2007 Subject: [Histonet] BCG source Message-ID: Hi everyone, I am looking for a vendor for BCG antibody - Dako used to carry it but it has been discontinued. Do any of you get it from a different source? Thanks, Erin From Rcartun <@t> harthosp.org Tue Aug 21 17:59:56 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Aug 21 18:00:10 2007 Subject: [Histonet] BCG source In-Reply-To: References: Message-ID: <46CB362C0200007700007A66@gwmail.harthosp.org> ViroStat (Portland, ME - www.virostat-inc.com) has several mycobacteria antibodies. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Martin, Erin" 08/21/07 6:36 PM >>> Hi everyone, I am looking for a vendor for BCG antibody - Dako used to carry it but it has been discontinued. Do any of you get it from a different source? Thanks, Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue Aug 21 19:17:14 2007 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Aug 21 19:17:21 2007 Subject: [Histonet] Histology Position Message-ID: <90354A475B420441B2A0396E5008D4965E1F5D@copc-sbs.COPC.local> Central Oregon Regional Pathology Services, Bend, OR is seeking an HT or HTL, ASCP registered or registry eligible preferred. Full-time position with rotating weekend coverage. Responsibilities include embedding, microtomy, routine staining, automated immunohistochemistry and special staining. Excellent benefits, including health insurance and retirement plan and competitive salary. Bend is located in central Oregon at the base of the Cascade Mountains. Hiking, backpacking, rock climbing, fishing and skiing opportunities abound. Central Oregon Regional Pathology Services, 1348 NE Cushing Dr., Bend, OR 97701 Attn: Pam Sylvester or fax resume to: (541) 389-5723 From mickie25 <@t> netzero.net Tue Aug 21 20:19:08 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Aug 21 20:19:23 2007 Subject: [Histonet] HELP In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA27056350C0@hpes1.HealthPartners.int> Message-ID: I agree with Mari Ann, It sound like xylene in the paraffin. That is one way to tell if the paraffin needs changing. If the middle paraffin feels greasy, then xylene has carried over from the first paraffin. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mari.ann.mailhiot@leica-microsystems.com Sent: Tuesday, August 21, 2007 3:15 PM To: Webb, Dorothy L Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELP HI Dorothy It sounds like xylene is in the wax. The other possibility could be water. Check the bottom of the paraffin tank and see if you have droplets. If you do then it is more than likely water. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Webb, Dorothy L" histonet@lists.utsouthwestern.edu Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] HELP 08/21/2007 12:19 PM What would cause the tissues when taken out of the processor at the end of the cycle to feel "slippery", almost greasy? I was off and back today and when the tech took the cassettes out of the processor, she mentioned this and they are not cutting well at all. Our processor was just changed yesterday and as far as I can tell from smell, all reagents are in order! Where should I start????? Thanks so much for answering this so quickly..I am stymied!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Aug 21 23:48:17 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Aug 21 23:48:27 2007 Subject: [Histonet] Help - Nuclear Fast Red In-Reply-To: References: Message-ID: Thank you Tony for recommending ethyl green! ----- Original Message ----- From: Tony Henwood Date: Tuesday, August 21, 2007 18:05 Subject: RE: [Histonet] Help - Nuclear Fast Red To: "Breeden, Sara" , histonet@lists.utsouthwestern.edu > Neutral red will work quite well, or ethyl green. > It is only a counterstain > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden,Sara > Sent: Wednesday, 22 August 2007 3:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Help - Nuclear Fast Red > > > I'm in the middle of a Von Kossa and my Nuclear Fast Red is missing! > What can I use as a substitute? Help! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential > and intended solely for the use of the individual or entity to > whom they are addressed. If you are not the intended recipient, > please delete it and notify the sender. > > Views expressed in this message and any attachments are those of > the individual sender, and are not necessarily the views of The > Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, > The Childrens Hospital at Westmead accepts no liability for any > consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From louise.renton <@t> gmail.com Wed Aug 22 05:18:32 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Aug 22 05:18:38 2007 Subject: [Histonet] thanks- per diem Message-ID: Thanks to all who responded to my per diem query. It has put costs into perspective, and given us a better feeling about what we will be able to afford. Not that we are stingy, but at $1 = 7.5 ZAR, it can get a bit pricey to shell out from one's own [pocket! Best regards to all and looking forward to meeting y'all stateside. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Cathy.Crumpton <@t> tuality.org Wed Aug 22 06:02:49 2007 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Wed Aug 22 06:02:56 2007 Subject: [Histonet] Cathy Crumpton is out of the office. Message-ID: I will be out of the office starting Wed 08/22/2007 and will not return until Mon 08/27/2007. If you have any histology concerns please route them to Connie Basinski during my absence. From b-frederick <@t> northwestern.edu Wed Aug 22 07:27:53 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 22 07:27:52 2007 Subject: [Histonet] HepPar Message-ID: <000101c7e4b7$dcee95c0$d00f7ca5@lurie.northwestern.edu> Hello all, Is HepPar the same as Heparan Sulfate proteoglycan/Perlecan? I need to know as I have to either order it or use the Perlecan antibody (just called perlecan) that I already have, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Aug 22 08:37:12 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Aug 22 08:41:53 2007 Subject: [Histonet] Retirement Message-ID: I will be retiring in two weeks after nearly 42 years in Histology at the Royal Infirmary, Lancaster, UK and I would just like to thanks everyone who has helped me over the past few years. It has been a pleasure to read and maybe help others and what a brilliant way of sharing information and improving what we do. To all those people out there in far away places with strange-sounding names - keep up the good work - you're worth it! Best wishes Jacqui malam DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From rjbuesa <@t> yahoo.com Wed Aug 22 08:49:13 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 22 08:49:17 2007 Subject: [Histonet] Retirement In-Reply-To: Message-ID: <816580.91048.qm@web61222.mail.yahoo.com> Jacqueline: Congratulations with your retirement but don't do what I guess your are going to do, namely, abandoning Histonet! Do as I do, I am also retired but had made the commitment of still trying to being helpful to others by participating in Histonet. As a matter of fact I am more helpful to others now than before retirement. You do that too. People with 42 years of experience are difficult to come by, and you should share it with everybody else. Ren? J. Malam Jacqueline wrote: I will be retiring in two weeks after nearly 42 years in Histology at the Royal Infirmary, Lancaster, UK and I would just like to thanks everyone who has helped me over the past few years. It has been a pleasure to read and maybe help others and what a brilliant way of sharing information and improving what we do. To all those people out there in far away places with strange-sounding names - keep up the good work - you're worth it! Best wishes Jacqui malam DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From LSebree <@t> uwhealth.org Wed Aug 22 09:09:30 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Aug 22 09:09:37 2007 Subject: [Histonet] HepPar In-Reply-To: <000101c7e4b7$dcee95c0$d00f7ca5@lurie.northwestern.edu> Message-ID: Bernice, HepPar, if it's the same as HepPar-1, is used for the diagnosis of hepatocelllular carcinoma so I don't believe that is what you want to use in place of Heparan Sulfate proteoglycan/Perlecan. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, August 22, 2007 7:28 AM To: 'histonet' Subject: [Histonet] HepPar Hello all, Is HepPar the same as Heparan Sulfate proteoglycan/Perlecan? I need to know as I have to either order it or use the Perlecan antibody (just called perlecan) that I already have, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Aug 22 09:21:38 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Aug 22 09:22:06 2007 Subject: [Histonet] Retirement Message-ID: I agree, do stay and give your input ever so often... Robyn >>> "Rene J Buesa" 8/22/2007 6:49 AM >>> Jacqueline: Congratulations with your retirement but don't do what I guess your are going to do, namely, abandoning Histonet! Do as I do, I am also retired but had made the commitment of still trying to being helpful to others by participating in Histonet. As a matter of fact I am more helpful to others now than before retirement. You do that too. People with 42 years of experience are difficult to come by, and you should share it with everybody else. Ren? J. Malam Jacqueline wrote: I will be retiring in two weeks after nearly 42 years in Histology at the Royal Infirmary, Lancaster, UK and I would just like to thanks everyone who has helped me over the past few years. It has been a pleasure to read and maybe help others and what a brilliant way of sharing information and improving what we do. To all those people out there in far away places with strange-sounding names - keep up the good work - you're worth it! Best wishes Jacqui malam DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Wed Aug 22 09:32:47 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Aug 22 09:32:53 2007 Subject: [Histonet] Region II Meeting in Delaware - Sept 6, 7, 8, 2007 Message-ID: <6.2.5.6.2.20070822101349.01c8d9a0@vet.upenn.edu> We are having a meeting for the Region II states all surrounding areas September 6th, 7th and 8th, 2007. IF you can not go to Denver consider joining us locally to get your CEUs and have a good time. The first day is geared to management type seminars and is open to everyone. Friday and Saturday will be general interest histology and related fields for all. We are having Innovex in for an animal IHC workshop as well a range of clinical IHC from beginner to advanced. We have the full program available with abstracts and registration. Please contact me or any state president (PA, NJ, MD, and the host state DE) for a copy by e-mail or mail. Vendors we still have a few booths if you wish to join us!! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From jnocito <@t> satx.rr.com Wed Aug 22 09:49:26 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 22 09:49:45 2007 Subject: [Histonet] microwaves in labs References: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> Message-ID: <006901c7e4cb$a3365750$d49eae18@yourxhtr8hvc4p> Jeanine, we use a home microwave that has a counter top fume hood over it. We calibrate the temp twice a year. While checking the temps, we also check for microwave leakage. Now, here I go again. I have my flame retardant suit on because I can already feel the flames coming. Some people are saying you have to use a laboratory grade microwave and not a home use one. I agree with this statement 100%, if you are processing tissue. If you are just using the microwave to perform special stains or the occasional heat slides, I don't see the need to spend all that money on a lab grade microwave. I've been using microwaves for specials for over 20 years and with the exception of the rare over heating of Carbol Fuschin (big mess) I've never had a microwave blow up. And you have to admit, the microwaves today are a lot safer than the ones 20 years ago. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, August 21, 2007 12:58 PM Subject: [Histonet] microwaves in labs Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Aug 22 09:55:20 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 22 09:55:33 2007 Subject: [Histonet] Retirement References: Message-ID: <008501c7e4cc$767e04a0$d49eae18@yourxhtr8hvc4p> Jacqui, congratulations on your retirement. I have to agree with everyone else, 42 years of experience is a wealth of knowledge. Please stay on, your knowledge is irreplaceable. We'll give you a couple of weeks off, but will expect you to return. At least do what I plan to do, keep my toes wet when I retire. Joe The Toe from across the pond ----- Original Message ----- From: "Malam Jacqueline" To: Sent: Wednesday, August 22, 2007 8:37 AM Subject: [Histonet] Retirement >I will be retiring in two weeks after nearly 42 years in Histology at the > Royal Infirmary, Lancaster, UK and I would just like to thanks everyone > who > has helped me over the past few years. It has been a pleasure to read and > maybe help others and what a brilliant way of sharing information and > improving what we do. To all those people out there in far away places > with > strange-sounding names - keep up the good work - you're worth it! > Best wishes > > Jacqui malam > > > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Wed Aug 22 09:53:42 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Aug 22 09:55:42 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <006901c7e4cb$a3365750$d49eae18@yourxhtr8hvc4p> References: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> <006901c7e4cb$a3365750$d49eae18@yourxhtr8hvc4p> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBF8C@LTA3VS003.ees.hhs.gov> Thanks for your feedback Joe. It is much appreciated. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, August 22, 2007 10:49 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microwaves in labs Jeanine, we use a home microwave that has a counter top fume hood over it. We calibrate the temp twice a year. While checking the temps, we also check for microwave leakage. Now, here I go again. I have my flame retardant suit on because I can already feel the flames coming. Some people are saying you have to use a laboratory grade microwave and not a home use one. I agree with this statement 100%, if you are processing tissue. If you are just using the microwave to perform special stains or the occasional heat slides, I don't see the need to spend all that money on a lab grade microwave. I've been using microwaves for specials for over 20 years and with the exception of the rare over heating of Carbol Fuschin (big mess) I've never had a microwave blow up. And you have to admit, the microwaves today are a lot safer than the ones 20 years ago. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, August 21, 2007 12:58 PM Subject: [Histonet] microwaves in labs Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mthomas <@t> littonlab.com Wed Aug 22 10:28:22 2007 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Wed Aug 22 10:26:43 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <34BB307EFC9A65429BBB49E330675F7202BDBF8C@LTA3VS003.ees.hhs.gov> References: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov><006901c7e4cb$a3365750$d49eae18@yourxhtr8hvc4p> <34BB307EFC9A65429BBB49E330675F7202BDBF8C@LTA3VS003.ees.hhs.gov> Message-ID: <002001c7e4d1$136c7040$9d35a8c0@LittonPath.local> Let the flaming begin! CAP Question ANP.27170 states "Are microwave devices used in accordance with manufacturer's instructions?" If you are CAP inspected you will need to have a microwave for Laboratory use. As far as safety is concerned, you still need to have them under a hood or vented to the outside, calibrate temps and check for leakage. You just get to pay more for the microwave. Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, August 22, 2007 9:54 AM To: Joe Nocito; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] microwaves in labs Thanks for your feedback Joe. It is much appreciated. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, August 22, 2007 10:49 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microwaves in labs Jeanine, we use a home microwave that has a counter top fume hood over it. We calibrate the temp twice a year. While checking the temps, we also check for microwave leakage. Now, here I go again. I have my flame retardant suit on because I can already feel the flames coming. Some people are saying you have to use a laboratory grade microwave and not a home use one. I agree with this statement 100%, if you are processing tissue. If you are just using the microwave to perform special stains or the occasional heat slides, I don't see the need to spend all that money on a lab grade microwave. I've been using microwaves for specials for over 20 years and with the exception of the rare over heating of Carbol Fuschin (big mess) I've never had a microwave blow up. And you have to admit, the microwaves today are a lot safer than the ones 20 years ago. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, August 21, 2007 12:58 PM Subject: [Histonet] microwaves in labs Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Aug 22 10:44:33 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Aug 22 10:44:39 2007 Subject: [Histonet] Fly heads In-Reply-To: <002001c7e4d1$136c7040$9d35a8c0@LittonPath.local> References: <34BB307EFC9A65429BBB49E330675F7202BDBF8C@LTA3VS003.ees.hhs.gov> <002001c7e4d1$136c7040$9d35a8c0@LittonPath.local> Message-ID: We have to paraffin process drosophila heads for H@E staining, any top tips from you fly guys/gals out there?. Many thanks Richard Edwards MRC TOXICOLOGY UNIT LEICESTER U.K. From rjbuesa <@t> yahoo.com Wed Aug 22 10:46:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 22 10:46:19 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <002001c7e4d1$136c7040$9d35a8c0@LittonPath.local> Message-ID: <157747.3992.qm@web61214.mail.yahoo.com> Marla: You are so right, but when dealing with "cooking" microwave ovens used for special stains: "he who is free from sin, cast the first stone". Ren? J. Marla Thomas wrote: Let the flaming begin! CAP Question ANP.27170 states "Are microwave devices used in accordance with manufacturer's instructions?" If you are CAP inspected you will need to have a microwave for Laboratory use. As far as safety is concerned, you still need to have them under a hood or vented to the outside, calibrate temps and check for leakage. You just get to pay more for the microwave. Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, August 22, 2007 9:54 AM To: Joe Nocito; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] microwaves in labs Thanks for your feedback Joe. It is much appreciated. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, August 22, 2007 10:49 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microwaves in labs Jeanine, we use a home microwave that has a counter top fume hood over it. We calibrate the temp twice a year. While checking the temps, we also check for microwave leakage. Now, here I go again. I have my flame retardant suit on because I can already feel the flames coming. Some people are saying you have to use a laboratory grade microwave and not a home use one. I agree with this statement 100%, if you are processing tissue. If you are just using the microwave to perform special stains or the occasional heat slides, I don't see the need to spend all that money on a lab grade microwave. I've been using microwaves for specials for over 20 years and with the exception of the rare over heating of Carbol Fuschin (big mess) I've never had a microwave blow up. And you have to admit, the microwaves today are a lot safer than the ones 20 years ago. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, August 21, 2007 12:58 PM Subject: [Histonet] microwaves in labs Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From b-frederick <@t> northwestern.edu Wed Aug 22 10:49:47 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 22 10:49:38 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <006901c7e4cb$a3365750$d49eae18@yourxhtr8hvc4p> Message-ID: <000701c7e4d4$11451c10$d00f7ca5@lurie.northwestern.edu> Don't you like decorating with carbol fuchsin? I've seen the inside of a microwave done in that lovely shade of fuchsia. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, August 22, 2007 9:49 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microwaves in labs Jeanine, we use a home microwave that has a counter top fume hood over it. We calibrate the temp twice a year. While checking the temps, we also check for microwave leakage. Now, here I go again. I have my flame retardant suit on because I can already feel the flames coming. Some people are saying you have to use a laboratory grade microwave and not a home use one. I agree with this statement 100%, if you are processing tissue. If you are just using the microwave to perform special stains or the occasional heat slides, I don't see the need to spend all that money on a lab grade microwave. I've been using microwaves for specials for over 20 years and with the exception of the rare over heating of Carbol Fuschin (big mess) I've never had a microwave blow up. And you have to admit, the microwaves today are a lot safer than the ones 20 years ago. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, August 21, 2007 12:58 PM Subject: [Histonet] microwaves in labs Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed Aug 22 10:55:30 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Aug 22 10:55:44 2007 Subject: FW: [Histonet] microwaves in labs In-Reply-To: Message-ID: I think we should all have a copy of Microwave Device Use in the Histology Laboratory; Approved Guideline. CLSI document GP28-A [ISBN 1-56238-563-1]. Also, in CAP question ANP.29430 Are microwave devices properly vented? The comment reads: This checklist question does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g., water, certain biological stains, paraffin sections). Hopefully this info will help,but probably it will only muddy the water. I better get back to my staining. Hopefully have not incited too much flaming. Patti Loykasek > > Let the flaming begin! > > CAP Question ANP.27170 states "Are microwave devices used in accordance with > manufacturer's instructions?" > > If you are CAP inspected you will need to have a microwave for Laboratory > use. > > As far as safety is concerned, you still need to have them under a hood or > vented to the outside, calibrate temps and check for leakage. You just get > to pay more for the microwave. > > Marla Thomas, HT(ASCP) > HIPAA/Compliance/IT Manager > Litton Pathology Associates, PC > 700 NW Hunter Drive > Blue Springs, MO 64015 > 816-229-6449, fax 816-874-4400 > > This e-mail, including attachments, may include confidential and/or > proprietary information, and may be used only by the person or entity to > which it is addressed. If the reader of this e-mail is not the intended > recipient or his/her authorized agent, the reader is hereby notified that > any dissemination, distribution or copying of this e-mail is prohibited. If > you have received this e-mail in error, please notify the sender by replying > to this message and delete this e-mail immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine (CDC/CCID/NCZVED) > Sent: Wednesday, August 22, 2007 9:54 AM > To: Joe Nocito; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] microwaves in labs > > Thanks for your feedback Joe. It is much appreciated. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Wednesday, August 22, 2007 10:49 AM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); > Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] microwaves in labs > > Jeanine, > we use a home microwave that has a counter top fume hood over it. We > calibrate the temp twice a year. While checking the temps, we also check > for microwave leakage. > Now, here I go again. I have my flame retardant suit on because I can > already feel the flames coming. > Some people are saying you have to use a laboratory grade microwave > and not a home use one. I agree with this statement 100%, if you are > processing tissue. If you are just using the microwave to perform > special stains or the occasional heat slides, I don't see the need to > spend all that money on a lab grade microwave. > I've been using microwaves for specials for over 20 years and with > the exception of the rare over heating of Carbol Fuschin (big mess) I've > never had a microwave blow up. And you have to admit, the microwaves > today are a lot safer than the ones 20 years ago. > > JTT > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: > Sent: Tuesday, August 21, 2007 12:58 PM > Subject: [Histonet] microwaves in labs > > > Hi all: > > I'd like to know how many of you out there use non-laboratory grade > microwaves for specials stains.......specifically for heating > methenamine-silver for a GMS. For those that do, are there any safety > issues of which you are aware? > > Thanks for your help! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------ End of Forwarded Message ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From sheila_adey <@t> hotmail.com Wed Aug 22 10:56:54 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Aug 22 10:57:05 2007 Subject: [Histonet] PCP IHC question Message-ID: Hi Netters, We have recently started using the PCP IHC method. Is anyone using a patient negative as with other standard IHCs ? We are also looking into doing our HPs by IHC. Do others use a retrieval for these bugs? Thanks in advance _________________________________________________________________ Put Your Face In Your Space with Windows Live Spaces http://spaces.live.com/?mkt=en-ca From Charles.Embrey <@t> carle.com Wed Aug 22 11:06:30 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Aug 22 11:06:36 2007 Subject: FW: [Histonet] microwaves in labs Message-ID: <44780C571F28624DBB446DE55C4D733A1FE528@EXCHANGEBE1.carle.com> -----Original Message----- From: Charles.Embrey Sent: Wednesday, August 22, 2007 11:04 AM To: 'Rene J Buesa' Subject: RE: [Histonet] microwaves in labs Actually CAP has softened their stand and Marla is no longer correct in her interpretation. We recently went through our CAP and were written up for our "home use" microwave. I used it for justification in ordering a new lab model and used the new oven invoice in my response to CAP to show correction of the write-up. A CAP rep actually called me to let me know that I could continue to use the oven I have as long as- #1: I placed it in a fume hood if heating anything that would give off harmful fumes and #2: I fulfilled the temp reproducibility checks. After all I had read I was very surprised to get this guidance directly from CAP Headquarters. They even said that I only needed to check for leakage if we felt there had been damage to the door. I am still buying my lab oven and will be doing processing and decal in it, but I will also now keep my old trusty kitchen microwave as well. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 22, 2007 10:46 AM To: Marla Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] microwaves in labs Marla: You are so right, but when dealing with "cooking" microwave ovens used for special stains: "he who is free from sin, cast the first stone". Ren? J. Marla Thomas wrote: Let the flaming begin! CAP Question ANP.27170 states "Are microwave devices used in accordance with manufacturer's instructions?" If you are CAP inspected you will need to have a microwave for Laboratory use. As far as safety is concerned, you still need to have them under a hood or vented to the outside, calibrate temps and check for leakage. You just get to pay more for the microwave. Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, August 22, 2007 9:54 AM To: Joe Nocito; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] microwaves in labs Thanks for your feedback Joe. It is much appreciated. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, August 22, 2007 10:49 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microwaves in labs Jeanine, we use a home microwave that has a counter top fume hood over it. We calibrate the temp twice a year. While checking the temps, we also check for microwave leakage. Now, here I go again. I have my flame retardant suit on because I can already feel the flames coming. Some people are saying you have to use a laboratory grade microwave and not a home use one. I agree with this statement 100%, if you are processing tissue. If you are just using the microwave to perform special stains or the occasional heat slides, I don't see the need to spend all that money on a lab grade microwave. I've been using microwaves for specials for over 20 years and with the exception of the rare over heating of Carbol Fuschin (big mess) I've never had a microwave blow up. And you have to admit, the microwaves today are a lot safer than the ones 20 years ago. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, August 21, 2007 12:58 PM Subject: [Histonet] microwaves in labs Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 22 11:19:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 22 11:19:17 2007 Subject: [Histonet] PCP IHC question In-Reply-To: Message-ID: <429716.92622.qm@web61224.mail.yahoo.com> There should always be a patient's section used as negative. Ren? J. sheila adey wrote: Hi Netters, We have recently started using the PCP IHC method. Is anyone using a patient negative as with other standard IHCs ? We are also looking into doing our HPs by IHC. Do others use a retrieval for these bugs? Thanks in advance _________________________________________________________________ Put Your Face In Your Space with Windows Live Spaces http://spaces.live.com/?mkt=en-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From jnocito <@t> satx.rr.com Wed Aug 22 11:21:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 22 11:21:37 2007 Subject: [Histonet] microwaves in labs References: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> <006901c7e4cb$a3365750$d49eae18@yourxhtr8hvc4p> <34BB307EFC9A65429BBB49E330675F7202BDBF8C@LTA3VS003.ees.hhs.gov> <002001c7e4d1$136c7040$9d35a8c0@LittonPath.local> Message-ID: <001901c7e4d8$78442830$d49eae18@yourxhtr8hvc4p> see, this is why I have problems with CAP. Describe manufacturer's instructions. Do these people have stock in microwave vendors? They throw out questions like this and then expect everyone to pay $35,000 for a microwave. It's ludicrous. I'll take the phase II and rebut it later. ----- Original Message ----- From: "Marla Thomas" To: Sent: Wednesday, August 22, 2007 10:28 AM Subject: RE: [Histonet] microwaves in labs > > Let the flaming begin! > > CAP Question ANP.27170 states "Are microwave devices used in accordance > with > manufacturer's instructions?" > > If you are CAP inspected you will need to have a microwave for Laboratory > use. > > As far as safety is concerned, you still need to have them under a hood or > vented to the outside, calibrate temps and check for leakage. You just get > to pay more for the microwave. > > Marla Thomas, HT(ASCP) > HIPAA/Compliance/IT Manager > Litton Pathology Associates, PC > 700 NW Hunter Drive > Blue Springs, MO 64015 > 816-229-6449, fax 816-874-4400 > > This e-mail, including attachments, may include confidential and/or > proprietary information, and may be used only by the person or entity to > which it is addressed. If the reader of this e-mail is not the intended > recipient or his/her authorized agent, the reader is hereby notified that > any dissemination, distribution or copying of this e-mail is prohibited. > If > you have received this e-mail in error, please notify the sender by > replying > to this message and delete this e-mail immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine (CDC/CCID/NCZVED) > Sent: Wednesday, August 22, 2007 9:54 AM > To: Joe Nocito; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] microwaves in labs > > Thanks for your feedback Joe. It is much appreciated. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Wednesday, August 22, 2007 10:49 AM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); > Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] microwaves in labs > > Jeanine, > we use a home microwave that has a counter top fume hood over it. We > calibrate the temp twice a year. While checking the temps, we also check > for microwave leakage. > Now, here I go again. I have my flame retardant suit on because I can > already feel the flames coming. > Some people are saying you have to use a laboratory grade microwave > and not a home use one. I agree with this statement 100%, if you are > processing tissue. If you are just using the microwave to perform > special stains or the occasional heat slides, I don't see the need to > spend all that money on a lab grade microwave. > I've been using microwaves for specials for over 20 years and with > the exception of the rare over heating of Carbol Fuschin (big mess) I've > never had a microwave blow up. And you have to admit, the microwaves > today are a lot safer than the ones 20 years ago. > > JTT > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: > Sent: Tuesday, August 21, 2007 12:58 PM > Subject: [Histonet] microwaves in labs > > > Hi all: > > I'd like to know how many of you out there use non-laboratory grade > microwaves for specials stains.......specifically for heating > methenamine-silver for a GMS. For those that do, are there any safety > issues of which you are aware? > > Thanks for your help! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Aug 22 11:24:56 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 22 11:25:04 2007 Subject: [Histonet] microwaves in labs References: Message-ID: <002401c7e4d8$fab78af0$d49eae18@yourxhtr8hvc4p> Patti, you're right. Just like we have to have copies of other CLSI regs, OSHA regs, ect. I'd rather spend $900 for a table top fume hood for a $100 microwave then spend $35K. I feel the heat The heat is on (wasn't that a song)? JTT ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Wednesday, August 22, 2007 10:55 AM Subject: FW: [Histonet] microwaves in labs > > I think we should all have a copy of Microwave Device Use in the > Histology > Laboratory; Approved Guideline. CLSI document GP28-A [ISBN 1-56238-563-1]. > Also, in CAP question ANP.29430 Are microwave devices properly vented? > The > comment reads: This checklist question does not apply if only > non-hazardous > reagents (and non-infectious specimens) are used in the device (e.g., > water, > certain biological stains, paraffin sections). Hopefully this info will > help,but probably it will only muddy the water. > I better get back to my staining. Hopefully have not incited too much > flaming. > > Patti Loykasek > > > > > > > >> >> Let the flaming begin! >> >> CAP Question ANP.27170 states "Are microwave devices used in accordance >> with >> manufacturer's instructions?" >> >> If you are CAP inspected you will need to have a microwave for Laboratory >> use. >> >> As far as safety is concerned, you still need to have them under a hood >> or >> vented to the outside, calibrate temps and check for leakage. You just >> get >> to pay more for the microwave. >> >> Marla Thomas, HT(ASCP) >> HIPAA/Compliance/IT Manager >> Litton Pathology Associates, PC >> 700 NW Hunter Drive >> Blue Springs, MO 64015 >> 816-229-6449, fax 816-874-4400 >> >> This e-mail, including attachments, may include confidential and/or >> proprietary information, and may be used only by the person or entity to >> which it is addressed. If the reader of this e-mail is not the intended >> recipient or his/her authorized agent, the reader is hereby notified that >> any dissemination, distribution or copying of this e-mail is prohibited. >> If >> you have received this e-mail in error, please notify the sender by >> replying >> to this message and delete this e-mail immediately. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Wednesday, August 22, 2007 9:54 AM >> To: Joe Nocito; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] microwaves in labs >> >> Thanks for your feedback Joe. It is much appreciated. >> >> >> Jeanine Bartlett >> Infectious Disease Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: Joe Nocito [mailto:jnocito@satx.rr.com] >> Sent: Wednesday, August 22, 2007 10:49 AM >> To: Bartlett, Jeanine (CDC/CCID/NCZVED); >> Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] microwaves in labs >> >> Jeanine, >> we use a home microwave that has a counter top fume hood over it. We >> calibrate the temp twice a year. While checking the temps, we also check >> for microwave leakage. >> Now, here I go again. I have my flame retardant suit on because I can >> already feel the flames coming. >> Some people are saying you have to use a laboratory grade microwave >> and not a home use one. I agree with this statement 100%, if you are >> processing tissue. If you are just using the microwave to perform >> special stains or the occasional heat slides, I don't see the need to >> spend all that money on a lab grade microwave. >> I've been using microwaves for specials for over 20 years and with >> the exception of the rare over heating of Carbol Fuschin (big mess) I've >> never had a microwave blow up. And you have to admit, the microwaves >> today are a lot safer than the ones 20 years ago. >> >> JTT >> ----- Original Message ----- >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: >> Sent: Tuesday, August 21, 2007 12:58 PM >> Subject: [Histonet] microwaves in labs >> >> >> Hi all: >> >> I'd like to know how many of you out there use non-laboratory grade >> microwaves for specials stains.......specifically for heating >> methenamine-silver for a GMS. For those that do, are there any safety >> issues of which you are aware? >> >> Thanks for your help! >> >> Jeanine Bartlett, BS, HT(ASCP)QIHC >> Centers for Disease Control and Prevention >> Infectious Diseases Pathology Branch >> 1600 Clifton Road, MS/G-32 >> 18/SB-114 >> Atlanta, GA 30333 >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------ End of Forwarded Message > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Wed Aug 22 11:42:14 2007 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Aug 22 11:42:35 2007 Subject: [Histonet] antibody diluent In-Reply-To: <81196.87505.qm@web50301.mail.re2.yahoo.com> References: <81196.87505.qm@web50301.mail.re2.yahoo.com> Message-ID: <46CC2F260200001A0000D6FE@GWIA2.umm.edu> Can antibody diluent go bad, and cause non-specific staining? I have no idea how old it is and theres no expiration date. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From rjbuesa <@t> yahoo.com Wed Aug 22 11:48:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 22 11:48:32 2007 Subject: [Histonet] antibody diluent In-Reply-To: <46CC2F260200001A0000D6FE@GWIA2.umm.edu> Message-ID: <594124.58397.qm@web61224.mail.yahoo.com> If used above the expiration date it will cause what you describe. You will have to buy a new one of prepare your own with the following recipe: 1- bovine serum--- 1 mL 2- PBS buffer --- 99 mL 3- Tween 20 ---- 0,05 mL 4- sodium azide --- 100 mg Store at 4?C Ren? J. Kimberly Tuttle wrote: Can antibody diluent go bad, and cause non-specific staining? I have no idea how old it is and theres no expiration date. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From NMargaryan <@t> childrensmemorial.org Wed Aug 22 12:24:10 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Aug 22 12:23:05 2007 Subject: [Histonet] RE: Histonet Digest, Vol 45, Issue 31 In-Reply-To: References: Message-ID: Dear Friends, colleagues and vendors, I am just back from the Animal IHC workshop in San Francisco. If you are doing any kind of Animal IHC this workshop is FOR YOU. There is always something to learn. I did fly all the way from Chicago and learn a new about Animal IHC. I am going to offer our lab in Chicago, IL in future for some other kind of IHC/ISH workshops for histo-people who lives close by. All vendors welcome to offer their products and trainings. Thanks to Zara Naser and her team from INNOVEX. Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 ------------------------------ Message: 13 Date: Tue, 21 Aug 2007 11:51:31 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Animal IHC workshop at Duke university To: "James Watson" , Message-ID: <002701c7e413$89245640$d49eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original James, welcome to the world of being flamed. But please don't stop your opinions because of a few people. If everyone thought alike, this would be a dull world. I thought as you did once and was going to remain silent. Even after the threat of loosing my job, I still gave my opinions. I even left for a while, but didn't feel right. I guess I just have too big of mouth to be kept silent. Welcome to the flamed club, but please be vocal about you opinions. Been there, done that JTT ----- Original Message ----- From: "James Watson" To: Sent: Tuesday, August 21, 2007 11:06 AM Subject: FW: [Histonet] Animal IHC workshop at Duke university I apologize for not stating my opinion clearly enough yesterday (it was Monday morning). I am not commenting on vendors giving courses. We would loose a lot of training if vendors did not give classes. I have helped arrange and advertised on the histonet vender taught classes myself. I did not say anything when a histo tech forwarded their advertisement about the course. I was not attacking this company. When I said I did not know this company I meant to say I do not know anyone from this company. I do use some of their products; their FC receptor block has worked extremely well on skin samples when staining for c-kit and phospho-c-kit in mast cells. My opinion is that vendors should not be able to post on the Histonet directly. I guess we have set a new precedent, vendors are allowed direct access to the Histonet, so Dako, Biocare, Ventana, Leica, and all the other vendors that offer courses should be allowed direct access to the Histonet. I will return to being a silent observer on the Histonet and only responding directly to individuals about questions. And yes I do know what the delete button is. Thank you. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of patsy ruegg Sent: Tue 8/21/2007 6:24 AM To: pmarcum@vet.upenn.edu; 'Bartlett, Jeanine (CDC/CCID/NCZVED)' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university Are you kidding me. We have come a long way from barring the vendors from doing ws at the NSH S/C or anywhere else. Without the support of our vendors (who by the way have some very knowledgeable speakers many of whom came from histology) the national and local conferences would not survive. In the past 10 years I have gotten as much or more from vendor sponsored courses as I have gotten from non-vendor courses. I say let them advertise all they want to offer FREE educational courses. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pmarcum@vet.upenn.edu Sent: Tuesday, August 21, 2007 6:36 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal IHC workshop at Duke university I was not going to make a comment as I know Jamie and this is one time I must disagree with him. We have invited Innovex to speak at the Region II meeting in Delaware and have had great response to the talk. I am one of the people doing animal IHC and I will admit I need help with some of the areas no one addresses with large animals. No One in the clincal companies offer enough help for this area so I for one can hardly wait to go to this workshop and get more information. I can always tailor it to my needs or know more about what to do with some issues. I will still be free to buy from whomever I wish as the class does not commit or any other attedee to the company itself. I hope they start to annouce here soon and I know it will be in my post to HistoNet about the meeting. Pam Marcum Quoting "Bartlett, Jeanine (CDC/CCID/NCZVED)" : > I agree. > > > Jeanine Bartlett > Infectious Disease Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie > Whitaker > Sent: Monday, August 20, 2007 4:46 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > That's what the delete button is for.... if I were doing much animal > tissue, I'd like to know when a free workshop with CEU's was close > by.... regardless of commercial content. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James > Watson > Sent: Monday, August 20, 2007 3:29 PM > To: INNOVEX BIOSCIENCES; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal IHC workshop at Duke university > > > I do not know this company, but I do believe that this is blatant > advertising of your products under the false pretense of an animal IHC > staining workshop. I did not think that the Histonet was to be used by > companies for advertising. I do not see other companies that give CEU > approved courses advertising on the Histonet, why should Innovex be > allowed to. > > Just my opinion on a busy Monday. > > James Watson HT ASCP > Facilities Manager of Histology > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of INNOVEX > BIOSCIENCES > Sent: Monday, August 20, 2007 1:04 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Animal IHC workshop at Duke university > > Animal IHC staining workshop, Duke university, September 11, 9-12 ( > morning session), CEU approved., a few spaces still available. This is > an educational workshop that targets performing animal IHC staining and > Mouse-on- Mouse IHC staining without background and under 2 hours. > Register on line at innvx.com or call 1-800-622-7808. From pmcardle <@t> ebsciences.com Wed Aug 22 12:30:50 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed Aug 22 12:31:01 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> References: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> Message-ID: <46CC72CA.4010704@ebsciences.com> And now a laboratory microwave vendor weighs in, so you can decide whether or not you want to read this, or what level of credibility we have (I'm getting out my Nomex flame-retardant suit): First of all, you're not stuck paying $35k for a lab microwave, even a model for processing. Second, please feel free to download our Microwave Companion, a purely "advancing-the-art" publication that does not attempt to sell anything at all: [1]http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf Third, lab managers should be at least as concerned about OSHA as CAP; see OSHA 29 CFR 1910.303(b)(2) which simply states that no food-grade microwave should be used for anything other than its intended purpose. Now, here's essentially a repeat of an earlier Histonet posting: Energy Beam Sciences, a pioneer in the use of microwaves in histology, has maintained for over a decade that consumer-grade microwaves have no place in a laboratory setting. Admittedly, over the years some users have managed to achieve varying levels of success in some "non-tissue-processing" applications like staining. However, I'd argue that a high number of failures due to this same approach have served to regress the art via anecdotal microwave "horror stories." Now that microwaves (both consumer-grade and true laboratory instrumentation) are in routine and widespread use in the histology laboratory, it should be unsurprising that CAP is taking this issue seriously. CAP is not alone; microwaves in histology have been receiving increased interest from standards institutes such as CLSI (see their publication GP-28A), and significantly, governmental agencies. Besides Europe's strict IVDD regulations, in the USA you have the aforementioned OSHA. It may be true that an external hood, fitted to a "kitchen" microwave, will remove vapors from the lab. But consumer grade microwaves have other limitations: they are often prone to hot and cold spots within their chambers, usually aren't designed or built to take the day-in-and-day-out demands of the histology laboratory, don't have calibrated output, and don't provide the means to measure output power, for example. Due to their long magnetron cycle times, there may be no difference at all between 15 seconds at 100% power and 15 seconds at 20% power. These are not deficiencies, given the proper context: heating leftovers or reheating cold coffee at home... an entirely different context from someone's biopsy, however. There's also the question of liability exposure. If your laboratory were party to any kind of lawsuit, whether as plaintiff or defendant, how comfortable would you be if it came out that an inexpensive consumer appliance were being used, rather than available, appropriate laboratory instrumentation, even if it had absolutely no bearing on the issue at hand? Now how does that $69 Wall-to-Wall-Mart microwave stack up? Late in 2005, after a series of conversations and e-mails with CAP representatives (and CLSI, whose publications CAP references), Energy Beam Sciences developed some recommendations to help with these new checklist items. An Acrobat file of said recommendations may be found on our website at [2]http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf (Note that the requirement for annual leakage measurement has been dropped, although it's still a good idea.) Also, feel free to browse our "library" section for articles, papers and protocols. Although I do all I can to sell our laboratory microwaves, I'm at least as interested in "advancing the art." To me, that translates to getting consumer microwaves out of labs, and true laboratory microwaves in, even if they're not ours. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 [3]pmcardle@ebsciences.com [4]www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain informati on that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, a nd it was probably my mistake anyway. So please do the right thing and make thi s e-mail go away. Thank you. Parker, Helayne wrote: I was wondering if anyone new what CAP require of us if we have a common household microwave that we use for special stains. Helayne Parker,HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list [5]Histonet@lists.utsouthwestern.edu [6]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [7]Histonet@lists.utsouthwestern.edu [8]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 [9]jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouthwestern.edu [11]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf 2. http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf 3. mailto:pmcardle@ebsciences.com 4. http://www.ebsciences.com/ 5. mailto:Histonet@lists.utsouthwestern.edu 6. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 7. mailto:Histonet@lists.utsouthwestern.edu 8. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 9. mailto:jeanine.bartlett@cdc.hhs.gov 10. mailto:Histonet@lists.utsouthwestern.edu 11. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Wed Aug 22 12:35:14 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Aug 22 12:35:27 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <46CC72CA.4010704@ebsciences.com> References: <34BB307EFC9A65429BBB49E330675F7202BDBF7E@LTA3VS003.ees.hhs.gov> <46CC72CA.4010704@ebsciences.com> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBF92@LTA3VS003.ees.hhs.gov> Since I started this whole thing let me reiterate my needs: I was simply asking if it would be a safety concern to heat a plastic coplin jar of methenamine silver for a GMS for 24 seconds once a week. We have a laboratory grade microwave but were looking to conserve space and I just wondered if anyone had first hand experience with this very narrow and specific limited use. Thanks to everyone for their input. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: Wednesday, August 22, 2007 1:31 PM To: Histonet (E-mail) Subject: Re: [Histonet] microwaves in labs And now a laboratory microwave vendor weighs in, so you can decide whether or not you want to read this, or what level of credibility we have (I'm getting out my Nomex flame-retardant suit): First of all, you're not stuck paying $35k for a lab microwave, even a model for processing. Second, please feel free to download our Microwave Companion, a purely "advancing-the-art" publication that does not attempt to sell anything at all: [1]http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf Third, lab managers should be at least as concerned about OSHA as CAP; see OSHA 29 CFR 1910.303(b)(2) which simply states that no food-grade microwave should be used for anything other than its intended purpose. Now, here's essentially a repeat of an earlier Histonet posting: Energy Beam Sciences, a pioneer in the use of microwaves in histology, has maintained for over a decade that consumer-grade microwaves have no place in a laboratory setting. Admittedly, over the years some users have managed to achieve varying levels of success in some "non-tissue-processing" applications like staining. However, I'd argue that a high number of failures due to this same approach have served to regress the art via anecdotal microwave "horror stories." Now that microwaves (both consumer-grade and true laboratory instrumentation) are in routine and widespread use in the histology laboratory, it should be unsurprising that CAP is taking this issue seriously. CAP is not alone; microwaves in histology have been receiving increased interest from standards institutes such as CLSI (see their publication GP-28A), and significantly, governmental agencies. Besides Europe's strict IVDD regulations, in the USA you have the aforementioned OSHA. It may be true that an external hood, fitted to a "kitchen" microwave, will remove vapors from the lab. But consumer grade microwaves have other limitations: they are often prone to hot and cold spots within their chambers, usually aren't designed or built to take the day-in-and-day-out demands of the histology laboratory, don't have calibrated output, and don't provide the means to measure output power, for example. Due to their long magnetron cycle times, there may be no difference at all between 15 seconds at 100% power and 15 seconds at 20% power. These are not deficiencies, given the proper context: heating leftovers or reheating cold coffee at home... an entirely different context from someone's biopsy, however. There's also the question of liability exposure. If your laboratory were party to any kind of lawsuit, whether as plaintiff or defendant, how comfortable would you be if it came out that an inexpensive consumer appliance were being used, rather than available, appropriate laboratory instrumentation, even if it had absolutely no bearing on the issue at hand? Now how does that $69 Wall-to-Wall-Mart microwave stack up? Late in 2005, after a series of conversations and e-mails with CAP representatives (and CLSI, whose publications CAP references), Energy Beam Sciences developed some recommendations to help with these new checklist items. An Acrobat file of said recommendations may be found on our website at [2]http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf (Note that the requirement for annual leakage measurement has been dropped, although it's still a good idea.) Also, feel free to browse our "library" section for articles, papers and protocols. Although I do all I can to sell our laboratory microwaves, I'm at least as interested in "advancing the art." To me, that translates to getting consumer microwaves out of labs, and true laboratory microwaves in, even if they're not ours. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 [3]pmcardle@ebsciences.com [4]www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain informati on that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, a nd it was probably my mistake anyway. So please do the right thing and make thi s e-mail go away. Thank you. Parker, Helayne wrote: I was wondering if anyone new what CAP require of us if we have a common household microwave that we use for special stains. Helayne Parker,HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list [5]Histonet@lists.utsouthwestern.edu [6]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [7]Histonet@lists.utsouthwestern.edu [8]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: Hi all: I'd like to know how many of you out there use non-laboratory grade microwaves for specials stains.......specifically for heating methenamine-silver for a GMS. For those that do, are there any safety issues of which you are aware? Thanks for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 [9]jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouthwestern.edu [11]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf 2. http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf 3. mailto:pmcardle@ebsciences.com 4. http://www.ebsciences.com/ 5. mailto:Histonet@lists.utsouthwestern.edu 6. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 7. mailto:Histonet@lists.utsouthwestern.edu 8. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 9. mailto:jeanine.bartlett@cdc.hhs.gov 10. mailto:Histonet@lists.utsouthwestern.edu 11. http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wstover <@t> vet.uga.edu Wed Aug 22 12:44:31 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Wed Aug 22 12:44:39 2007 Subject: [Histonet] CD3 and CD79 on Canine Lymphs Noses Message-ID: Are there anyone out there doing CD3 and CD79 on canine lymph nodes touch preps? If so..help with a good protocol? Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From wstover <@t> vet.uga.edu Wed Aug 22 12:45:38 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Wed Aug 22 12:45:44 2007 Subject: [Histonet] Correction: Canine Lymph Nodes... Message-ID: Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From RCazares <@t> schosp.org Wed Aug 22 12:52:28 2007 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Aug 22 12:52:34 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <002401c7e4d8$fab78af0$d49eae18@yourxhtr8hvc4p> References: <002401c7e4d8$fab78af0$d49eae18@yourxhtr8hvc4p> Message-ID: <913FAC2B773C19488E26AE6572180FA509BF0283@exch01.schosp.org> Yup. And so was "Some Like It Hot" by Van Halen. I think that song was about you Joe, feel the heat. Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, August 22, 2007 11:25 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] microwaves in labs Patti, you're right. Just like we have to have copies of other CLSI regs, OSHA regs, ect. I'd rather spend $900 for a table top fume hood for a $100 microwave then spend $35K. I feel the heat The heat is on (wasn't that a song)? JTT ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Wednesday, August 22, 2007 10:55 AM Subject: FW: [Histonet] microwaves in labs > > I think we should all have a copy of Microwave Device Use in the > Histology > Laboratory; Approved Guideline. CLSI document GP28-A [ISBN 1-56238-563-1]. > Also, in CAP question ANP.29430 Are microwave devices properly vented? > The > comment reads: This checklist question does not apply if only > non-hazardous > reagents (and non-infectious specimens) are used in the device (e.g., > water, > certain biological stains, paraffin sections). Hopefully this info will > help,but probably it will only muddy the water. > I better get back to my staining. Hopefully have not incited too much > flaming. > > Patti Loykasek > > > > > > > >> >> Let the flaming begin! >> >> CAP Question ANP.27170 states "Are microwave devices used in accordance >> with >> manufacturer's instructions?" >> >> If you are CAP inspected you will need to have a microwave for Laboratory >> use. >> >> As far as safety is concerned, you still need to have them under a hood >> or >> vented to the outside, calibrate temps and check for leakage. You just >> get >> to pay more for the microwave. >> >> Marla Thomas, HT(ASCP) >> HIPAA/Compliance/IT Manager >> Litton Pathology Associates, PC >> 700 NW Hunter Drive >> Blue Springs, MO 64015 >> 816-229-6449, fax 816-874-4400 >> >> This e-mail, including attachments, may include confidential and/or >> proprietary information, and may be used only by the person or entity to >> which it is addressed. If the reader of this e-mail is not the intended >> recipient or his/her authorized agent, the reader is hereby notified that >> any dissemination, distribution or copying of this e-mail is prohibited. >> If >> you have received this e-mail in error, please notify the sender by >> replying >> to this message and delete this e-mail immediately. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Wednesday, August 22, 2007 9:54 AM >> To: Joe Nocito; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] microwaves in labs >> >> Thanks for your feedback Joe. It is much appreciated. >> >> >> Jeanine Bartlett >> Infectious Disease Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: Joe Nocito [mailto:jnocito@satx.rr.com] >> Sent: Wednesday, August 22, 2007 10:49 AM >> To: Bartlett, Jeanine (CDC/CCID/NCZVED); >> Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] microwaves in labs >> >> Jeanine, >> we use a home microwave that has a counter top fume hood over it. We >> calibrate the temp twice a year. While checking the temps, we also check >> for microwave leakage. >> Now, here I go again. I have my flame retardant suit on because I can >> already feel the flames coming. >> Some people are saying you have to use a laboratory grade microwave >> and not a home use one. I agree with this statement 100%, if you are >> processing tissue. If you are just using the microwave to perform >> special stains or the occasional heat slides, I don't see the need to >> spend all that money on a lab grade microwave. >> I've been using microwaves for specials for over 20 years and with >> the exception of the rare over heating of Carbol Fuschin (big mess) I've >> never had a microwave blow up. And you have to admit, the microwaves >> today are a lot safer than the ones 20 years ago. >> >> JTT >> ----- Original Message ----- >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: >> Sent: Tuesday, August 21, 2007 12:58 PM >> Subject: [Histonet] microwaves in labs >> >> >> Hi all: >> >> I'd like to know how many of you out there use non-laboratory grade >> microwaves for specials stains.......specifically for heating >> methenamine-silver for a GMS. For those that do, are there any safety >> issues of which you are aware? >> >> Thanks for your help! >> >> Jeanine Bartlett, BS, HT(ASCP)QIHC >> Centers for Disease Control and Prevention >> Infectious Diseases Pathology Branch >> 1600 Clifton Road, MS/G-32 >> 18/SB-114 >> Atlanta, GA 30333 >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------ End of Forwarded Message > > > > ------------------------------------------------------------------------ - > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From buri <@t> seattleskincancer.com Wed Aug 22 12:53:58 2007 From: buri <@t> seattleskincancer.com (Beth Uri) Date: Wed Aug 22 12:54:10 2007 Subject: [Histonet] part-time job Seattle Message-ID: <001c01c7e4e5$6b16f9a0$2500000a@corp.birkby.com> I am looking for someone to cut frozen sections for Mohs surgery part-time M-Th. This is a perfect job for someone who just wants to pick-up a few extra hours per week. Hours and even the days are negotiable. I am willing to train. If interested or if you want more information please give me a call at 206-215-3369. Thanks Beth Seattle Skin Cancer Center From Linke_Noelle <@t> Allergan.com Wed Aug 22 12:55:37 2007 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Wed Aug 22 12:56:11 2007 Subject: [Histonet] Correction: Canine Lymph Nodes... In-Reply-To: Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60502EA93@IRMAIL132.irvine.allergan.com> Hi Wanda, CD3-rabbit monoclonal from LabVision and the rabbit polyclonal from Dako both work on K9 (ffpe) CD79a from LabVision does not work, and I do not have a substitute to recommend.. Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda Stover Sent: Wednesday, August 22, 2007 10:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Correction: Canine Lymph Nodes... Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From agoldstein <@t> KaiserAssociates.com Wed Aug 22 13:41:55 2007 From: agoldstein <@t> KaiserAssociates.com (Adam Goldstein) Date: Wed Aug 22 13:40:18 2007 Subject: [Histonet] Histology listservers/forums in Europe Message-ID: Hi, I am looking for histology listservers or web forums that cater specifically to Europeans (especially in UK, France, Germany, and Spain). Does anyone know of any, or have ideas on the best places to look for them? I realize that Histonet has international subscribers, but I don't know the actual European contingent that is represented here. Maybe someone has a better idea of this than I do. Thank you! Adam Goldstein Researcher Washington, DC From shive003 <@t> umn.edu Wed Aug 22 14:07:03 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Aug 22 14:07:06 2007 Subject: [Histonet] Correction: Canine Lymph Nodes... References: Message-ID: <00a001c7e4ef$a03f2d00$a1065486@auxs.umn.edu> Lab Vision's CD20, rabbit polyclonal (cat. # RB9013-P) works on canine, feline, etc. No pretreatment is necessary! Jan Shivers U of MN Vet Diag Lab St. Paul, MN shive003@umn.edu ----- Original Message ----- From: "Wanda Stover" To: Sent: Wednesday, August 22, 2007 12:45 PM Subject: [Histonet] Correction: Canine Lymph Nodes... > > > > > Wanda Stover > > UGA VET MED > > Histology > > Athens, Ga. 30602 > > 706 583-0474 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Wed Aug 22 15:03:43 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 22 15:03:55 2007 Subject: [Histonet] microwaves in labs References: <002401c7e4d8$fab78af0$d49eae18@yourxhtr8hvc4p> <913FAC2B773C19488E26AE6572180FA509BF0283@exch01.schosp.org> Message-ID: <001f01c7e4f7$8b798340$d49eae18@yourxhtr8hvc4p> my toes are getting warm. ----- Original Message ----- From: "Cazares, Ruth" To: "histonet" Sent: Wednesday, August 22, 2007 12:52 PM Subject: RE: [Histonet] microwaves in labs Yup. And so was "Some Like It Hot" by Van Halen. I think that song was about you Joe, feel the heat. Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, August 22, 2007 11:25 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] microwaves in labs Patti, you're right. Just like we have to have copies of other CLSI regs, OSHA regs, ect. I'd rather spend $900 for a table top fume hood for a $100 microwave then spend $35K. I feel the heat The heat is on (wasn't that a song)? JTT ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Wednesday, August 22, 2007 10:55 AM Subject: FW: [Histonet] microwaves in labs > > I think we should all have a copy of Microwave Device Use in the > Histology > Laboratory; Approved Guideline. CLSI document GP28-A [ISBN 1-56238-563-1]. > Also, in CAP question ANP.29430 Are microwave devices properly vented? > The > comment reads: This checklist question does not apply if only > non-hazardous > reagents (and non-infectious specimens) are used in the device (e.g., > water, > certain biological stains, paraffin sections). Hopefully this info will > help,but probably it will only muddy the water. > I better get back to my staining. Hopefully have not incited too much > flaming. > > Patti Loykasek > > > > > > > >> >> Let the flaming begin! >> >> CAP Question ANP.27170 states "Are microwave devices used in accordance >> with >> manufacturer's instructions?" >> >> If you are CAP inspected you will need to have a microwave for Laboratory >> use. >> >> As far as safety is concerned, you still need to have them under a hood >> or >> vented to the outside, calibrate temps and check for leakage. You just >> get >> to pay more for the microwave. >> >> Marla Thomas, HT(ASCP) >> HIPAA/Compliance/IT Manager >> Litton Pathology Associates, PC >> 700 NW Hunter Drive >> Blue Springs, MO 64015 >> 816-229-6449, fax 816-874-4400 >> >> This e-mail, including attachments, may include confidential and/or >> proprietary information, and may be used only by the person or entity to >> which it is addressed. If the reader of this e-mail is not the intended >> recipient or his/her authorized agent, the reader is hereby notified that >> any dissemination, distribution or copying of this e-mail is prohibited. >> If >> you have received this e-mail in error, please notify the sender by >> replying >> to this message and delete this e-mail immediately. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) >> Sent: Wednesday, August 22, 2007 9:54 AM >> To: Joe Nocito; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] microwaves in labs >> >> Thanks for your feedback Joe. It is much appreciated. >> >> >> Jeanine Bartlett >> Infectious Disease Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: Joe Nocito [mailto:jnocito@satx.rr.com] >> Sent: Wednesday, August 22, 2007 10:49 AM >> To: Bartlett, Jeanine (CDC/CCID/NCZVED); >> Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] microwaves in labs >> >> Jeanine, >> we use a home microwave that has a counter top fume hood over it. We >> calibrate the temp twice a year. While checking the temps, we also check >> for microwave leakage. >> Now, here I go again. I have my flame retardant suit on because I can >> already feel the flames coming. >> Some people are saying you have to use a laboratory grade microwave >> and not a home use one. I agree with this statement 100%, if you are >> processing tissue. If you are just using the microwave to perform >> special stains or the occasional heat slides, I don't see the need to >> spend all that money on a lab grade microwave. >> I've been using microwaves for specials for over 20 years and with >> the exception of the rare over heating of Carbol Fuschin (big mess) I've >> never had a microwave blow up. And you have to admit, the microwaves >> today are a lot safer than the ones 20 years ago. >> >> JTT >> ----- Original Message ----- >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: >> Sent: Tuesday, August 21, 2007 12:58 PM >> Subject: [Histonet] microwaves in labs >> >> >> Hi all: >> >> I'd like to know how many of you out there use non-laboratory grade >> microwaves for specials stains.......specifically for heating >> methenamine-silver for a GMS. For those that do, are there any safety >> issues of which you are aware? >> >> Thanks for your help! >> >> Jeanine Bartlett, BS, HT(ASCP)QIHC >> Centers for Disease Control and Prevention >> Infectious Diseases Pathology Branch >> 1600 Clifton Road, MS/G-32 >> 18/SB-114 >> Atlanta, GA 30333 >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------ End of Forwarded Message > > > > ------------------------------------------------------------------------ - > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Aug 22 15:22:56 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Aug 22 15:23:13 2007 Subject: [Histonet] Re: Help - Nuclear Fast Red (Breeden, Sara) Message-ID: <001701c7e4fa$3a25ef30$4101a8c0@carlba65530bda> Yep...nice one Tony. In my XP, I found Ethyl green less "volatile" than Neutral red, to the dehydration stages. I'd be grateful for any comments on my opinion. Carl From bakevictoria <@t> gmail.com Wed Aug 22 15:26:48 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Aug 22 15:26:57 2007 Subject: [Histonet] microwaves in labs In-Reply-To: <001f01c7e4f7$8b798340$d49eae18@yourxhtr8hvc4p> References: <002401c7e4d8$fab78af0$d49eae18@yourxhtr8hvc4p> <913FAC2B773C19488E26AE6572180FA509BF0283@exch01.schosp.org> <001f01c7e4f7$8b798340$d49eae18@yourxhtr8hvc4p> Message-ID: <4f016b690708221326q458d359k2681c6b89536c103@mail.gmail.com> Joe your toes should be real toastie! "The Heat Is On" was written by Glen Frey (of the Eagles) for the movie Beverly Hills Cop I. It's the song I used to play when working on a deadline in the lab or on a paper (cranked real high when alone) as I just loved the lyrics and the sax! Vikki Baker On 8/22/07, Joe Nocito wrote: > my toes are getting warm. > > ----- Original Message ----- > From: "Cazares, Ruth" > To: "histonet" > Sent: Wednesday, August 22, 2007 12:52 PM > Subject: RE: [Histonet] microwaves in labs > > > Yup. And so was "Some Like It Hot" by Van Halen. I think that song was > about you Joe, feel the heat. > > > Ruth Cazares, Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL 60625 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Wednesday, August 22, 2007 11:25 AM > To: Patti Loykasek; histonet > Subject: Re: [Histonet] microwaves in labs > > Patti, > you're right. Just like we have to have copies of other CLSI regs, OSHA > regs, ect. > I'd rather spend $900 for a table top fume hood for a $100 microwave > then spend $35K. > > I feel the heat The heat is on (wasn't that a song)? > > JTT > ----- Original Message ----- > From: "Patti Loykasek" > To: "histonet" > Sent: Wednesday, August 22, 2007 10:55 AM > Subject: FW: [Histonet] microwaves in labs > > > > > > I think we should all have a copy of Microwave Device Use in the > > Histology > > Laboratory; Approved Guideline. CLSI document GP28-A [ISBN > 1-56238-563-1]. > > Also, in CAP question ANP.29430 Are microwave devices properly > vented? > > The > > comment reads: This checklist question does not apply if only > > non-hazardous > > reagents (and non-infectious specimens) are used in the device (e.g., > > water, > > certain biological stains, paraffin sections). Hopefully this info > will > > help,but probably it will only muddy the water. > > I better get back to my staining. Hopefully have not incited too much > > flaming. > > > > Patti Loykasek > > > > > > > > > > > > > > > >> > >> Let the flaming begin! > >> > >> CAP Question ANP.27170 states "Are microwave devices used in > accordance > >> with > >> manufacturer's instructions?" > >> > >> If you are CAP inspected you will need to have a microwave for > Laboratory > >> use. > >> > >> As far as safety is concerned, you still need to have them under a > hood > >> or > >> vented to the outside, calibrate temps and check for leakage. You > just > >> get > >> to pay more for the microwave. > >> > >> Marla Thomas, HT(ASCP) > >> HIPAA/Compliance/IT Manager > >> Litton Pathology Associates, PC > >> 700 NW Hunter Drive > >> Blue Springs, MO 64015 > >> 816-229-6449, fax 816-874-4400 > >> > >> This e-mail, including attachments, may include confidential and/or > >> proprietary information, and may be used only by the person or entity > to > >> which it is addressed. If the reader of this e-mail is not the > intended > >> recipient or his/her authorized agent, the reader is hereby notified > that > >> any dissemination, distribution or copying of this e-mail is > prohibited. > >> If > >> you have received this e-mail in error, please notify the sender by > >> replying > >> to this message and delete this e-mail immediately. > >> > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, > >> Jeanine (CDC/CCID/NCZVED) > >> Sent: Wednesday, August 22, 2007 9:54 AM > >> To: Joe Nocito; Histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] microwaves in labs > >> > >> Thanks for your feedback Joe. It is much appreciated. > >> > >> > >> Jeanine Bartlett > >> Infectious Disease Pathology Branch > >> (404) 639-3590 > >> jeanine.bartlett@cdc.hhs.gov > >> > >> > >> -----Original Message----- > >> From: Joe Nocito [mailto:jnocito@satx.rr.com] > >> Sent: Wednesday, August 22, 2007 10:49 AM > >> To: Bartlett, Jeanine (CDC/CCID/NCZVED); > >> Histonet@lists.utsouthwestern.edu > >> Subject: Re: [Histonet] microwaves in labs > >> > >> Jeanine, > >> we use a home microwave that has a counter top fume hood over it. We > >> calibrate the temp twice a year. While checking the temps, we also > check > >> for microwave leakage. > >> Now, here I go again. I have my flame retardant suit on because I can > >> already feel the flames coming. > >> Some people are saying you have to use a laboratory grade microwave > >> and not a home use one. I agree with this statement 100%, if you are > >> processing tissue. If you are just using the microwave to perform > >> special stains or the occasional heat slides, I don't see the need to > >> spend all that money on a lab grade microwave. > >> I've been using microwaves for specials for over 20 years and with > >> the exception of the rare over heating of Carbol Fuschin (big mess) > I've > >> never had a microwave blow up. And you have to admit, the microwaves > >> today are a lot safer than the ones 20 years ago. > >> > >> JTT > >> ----- Original Message ----- > >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > >> To: > >> Sent: Tuesday, August 21, 2007 12:58 PM > >> Subject: [Histonet] microwaves in labs > >> > >> > >> Hi all: > >> > >> I'd like to know how many of you out there use non-laboratory grade > >> microwaves for specials stains.......specifically for heating > >> methenamine-silver for a GMS. For those that do, are there any safety > >> issues of which you are aware? > >> > >> Thanks for your help! > >> > >> Jeanine Bartlett, BS, HT(ASCP)QIHC > >> Centers for Disease Control and Prevention > >> Infectious Diseases Pathology Branch > >> 1600 Clifton Road, MS/G-32 > >> 18/SB-114 > >> Atlanta, GA 30333 > >> (404) 639-3590 > >> jeanine.bartlett@cdc.hhs.gov > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------ End of Forwarded Message > > > > > > > > > ------------------------------------------------------------------------ > - > > This e-mail message, including any attachments, is for the sole use of > > the intended recipients and may contain privileged information. Any > > unauthorized review, use, disclosure or distribution is prohibited. If > > you are not the intended recipient, please contact the sender by > e-mail > > and destroy all copies of the original message, or you may call > PhenoPath > > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged and > confidential. If the reader of this message is not the intended recipient, > please notify the sender immediately by replying to this message and then > delete it from your system. Any review, dissemination, distribution, or > reproduction of this message by unintended recipients is strictly prohibited > and may be subject to legal restriction. > > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From agustinvictor <@t> gmail.com Wed Aug 22 16:10:30 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Wed Aug 22 16:10:30 2007 Subject: [Histonet] Fw: Thiocarbohydrazide Message-ID: <004d01c7e500$e093ece0$590215ac@Pentium4> ----- Original Message ----- From: Agustin Chertcoff To: histonet@lists.utsouthwestern.edu Sent: Monday, August 13, 2007 5:47 PM Subject: Thiocarbohydrazide Hi Histoneters! I need a protocole for OTO Technics (Osmium tetroxide, Thiocarbohydrazide) for Electron Microcoscopy, specially as preparing the Thiocarbohidrazide solution and the correct temperature Thanks in advanced ! Ht Agustin Victor Chertcoff Electron Microscopy Service National Institute of Microbiology C G Malbran Buenos Aires Argentina From rschoon <@t> email.unc.edu Wed Aug 22 16:36:25 2007 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Wed Aug 22 16:37:07 2007 Subject: [Histonet] anti-human antibodies In-Reply-To: References: Message-ID: <46CCAC59.7010701@email.unc.edu> Fellow 'netters, I have been looking for a anti-human nuclear antibody that will work on FFPE tissue that will be specific for human tissue only, no cross reactivity to other species. Thought that I found one from Chemicon but I cannot seem to be able to get it to work anymore. Had it working 8 months ago but not since. I've used all of the standard antibody search engines as well as telephone calls to various venders. Maybe I'm using the wrong search parameters???? If anyone has a procedure up and running I'd appreciate any information you would be willing to share. Vendors feel free to reply. Many thanks in advance Bob Robert Schoonhoven From detmar <@t> mshri.on.ca Wed Aug 22 16:42:49 2007 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Wed Aug 22 16:43:00 2007 Subject: [Histonet] mouse histology textbook Message-ID: Hi all. I am posting this message for a friend that works on mouse imaging here at Toronto. Here is her message: MICe (the institute she works in) wants to buy a histology textbook to help us "figure some stuff out". We just want basic info on histology, info on which stains are best for which tissues, and hopefully some detail on histological stains for the brain. I'm pretty sure they would like something solely for mice, but a general histology book would probably do well, too. Thanks! Jacqui Detmar Samuel Lunenfeld Research Institute, 600 University Avenue Toronto, Ontario, Canada From AnthonyH <@t> chw.edu.au Wed Aug 22 18:36:55 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 22 18:37:49 2007 Subject: [Histonet] Re: Help - Nuclear Fast Red (Breeden, Sara) Message-ID: I suppose we only use it as a counterstain, so the less intrusive the better. It is good to have a panel of simple counterstains available for any situation that may arise Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs Sent: Thursday, 23 August 2007 6:23 AM To: Histonet Subject: [Histonet] Re: Help - Nuclear Fast Red (Breeden, Sara) Yep...nice one Tony. In my XP, I found Ethyl green less "volatile" than Neutral red, to the dehydration stages. I'd be grateful for any comments on my opinion. Carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jkiernan <@t> uwo.ca Thu Aug 23 00:09:54 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Aug 23 00:10:05 2007 Subject: [Histonet] Fw: Thiocarbohydrazide In-Reply-To: <004d01c7e500$e093ece0$590215ac@Pentium4> References: <004d01c7e500$e093ece0$590215ac@Pentium4> Message-ID: The answer to your question is simple. Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless. Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results. John Kiernan Anatomy, UWO London, Canada --- > ----- Original Message ----- > From: Agustin Chertcoff > To: histonet@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 5:47 PM > Subject: Thiocarbohydrazide > > > Hi Histoneters! > I need a protocole for OTO Technics (Osmium tetroxide, > Thiocarbohydrazide) for Electron Microcoscopy, specially as > preparing the Thiocarbohidrazide solution and the correct > temperature > > Thanks in advanced ! > > Ht Agustin Victor Chertcoff > Electron Microscopy Service > National Institute of Microbiology C G Malbran > Buenos Aires > Argentina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Aug 23 02:03:38 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Aug 23 02:03:45 2007 Subject: [Histonet] Retirement Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EB8E@wahtntex2.waht.swest.nhs.uk> Congratulations Jacqueline, I guess I'll buck the trend and suggest that when you leave, don't look back and maybe never enter the Listserv again. You've done your bit over 42 years and now you can fly, don't let the shackles of the past ground you. Best wishes. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; What lies behind us and what lies before us are small matters compared to what lies within us. --Ralph Waldo Emerson his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From drkwolfe <@t> telus.net Thu Aug 23 04:10:26 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Thu Aug 23 04:10:39 2007 Subject: [Histonet] Book Search - Donations Message-ID: Fellow Histonetters I know I brought this topic up once before, but, as I did not meet up with any success last time, I'd like to try my small request one last time. I am currently working on my Histotechnology degree. I have been working exclusively with a small research firm in Histology. My studies cover some of what I need to know now, but not sufficiently yet. The basis of my request is to see if anyone can either Donate or Loan these books to myself. I can not really afford to purchase the books New (or even used at most places) as I have many other additional text books that need to be purchased that arent on the budget. There are a number of excellent textbooks out there. There are 3 in particular that I would really like to get a copy of (old or current, beggers can not be choosers) are most important to bringing my skills and knowledge up to par. These texts are: * Basic Histology Text & Atlas (10th Edition) (Junqueira & Carneiro)Lange 2003 * Theory and Practice of Histological Techniques 5th Edition, Edited by John Bancroft and Marilyn Gamble * Lynch's Medical Laboratory Technology (3rd edition or better) Volume 1 and 2 if Posibile And finally any textbooks relating to Vet Medicine, Zoology, Gross Human Anatomy, and any atlases for mouse, rat, human compatable structures, etc. I am a student, and not making the real big bucks yet. So therefore I am asking anyone if they could possibly donate or Loan any of these text books. The books can then be either returned to their original owners, or they can be donated to the next student in need of some additonal reading. If anyone is willing to Donate these books, please E-Mail me direct for the shipping information. Thank You for your time and generousity. Joe Kapler I'm an outsider outside of everything I'm an outsider outside of everything I'm an outsider outside of everything Everything you know Everything you know It disturbs me so No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.2/967 - Release Date: 8/22/2007 18:51 From ian.montgomery <@t> bio.gla.ac.uk Thu Aug 23 04:32:20 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Aug 23 04:32:27 2007 Subject: [Histonet] Retirement. Message-ID: <001a01c7e568$80c7b680$6424d182@IBLS.GLA.AC.UK> Jacqueline, I fully agree with Kemlo, you've done the time, move on. I'm rapidly approaching retirement and then it will be burn the lab coat and start afresh. Colleges and Universities offer all sorts of interesting courses, things that you always fancied but never had the time. Go for it girl, you know it makes sense. As a wee aside, I've just reached the magic 60 and have a travel pass from the Scottish Executive that allows free travel on buses throughout Scotland. How do you fancy this for the US? New York to San Francisco and all points between up and down. Might take several years but who cares, you'll have the time and think of the fun. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From drkwolfe <@t> telus.net Thu Aug 23 04:46:36 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Thu Aug 23 04:46:44 2007 Subject: [Histonet] Retirement In-Reply-To: Message-ID: Jacqui Congratulations on your retirement. 42 years in the profession is amazing. I've seen a number of suggestions made, and I think my preference out of the group of them... Take time off for yourself and your family. Enjoy the time you have now, and live each day as the only day... As for the rest of us on Histonet, especially us young (well not so young in my case) students, we will continue to look forward to any future gems of wisdom that you may care to pass along once you find time to settle and relax, then come back occassionaly to vist the old gang here on Histonet. Joe Kapler I'm an outsider outside of everything I'm an outsider outside of everything I'm an outsider outside of everything Everything you know Everything you know It disturbs me so -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Malam Jacqueline Sent: Wednesday, August 22, 2007 07:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement I will be retiring in two weeks after nearly 42 years in Histology at the Royal Infirmary, Lancaster, UK and I would just like to thanks everyone who has helped me over the past few years. It has been a pleasure to read and maybe help others and what a brilliant way of sharing information and improving what we do. To all those people out there in far away places with strange-sounding names - keep up the good work - you're worth it! Best wishes Jacqui malam DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.2/967 - Release Date: 8/22/2007 18:51 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.2/967 - Release Date: 8/22/2007 18:51 From Jane.Moose <@t> NewberryHospital.net Thu Aug 23 08:00:36 2007 From: Jane.Moose <@t> NewberryHospital.net (Jane C. Moose) Date: Thu Aug 23 08:02:45 2007 Subject: [Histonet] FW: faded H&E stain Message-ID: <8BB5FC36DDA373488E60177757BBD6980F298D@ncmhexchbe01.NewberryHospital.net> Recently we have experienced sporadic problems with our routine H&E stains of some small GI biopsies and Ultrasound guided needle biopsies of breast tissue. The stains appear faded and almost unreadable. Other tissue processed at the same time, cut, oven dried, and stained in the same rack of slides (we hand stain) are stained appropriately. Usually these are from larger sections of tissue such as appendix, gallbladder, skin, uterus, colon segment, etc. This faded stain may happen one day and then not again for several and then happen again just for certain tissues that day. We are working with the "collecting techs" to ensure that the tissue is appropriately handled before we receive it. For breast tissue, the technique that has been used was to drop the biopsy in sterile water just to remove it from the syringe and transfer immediately to formalin. This has worked well for quite some time. Today however, we have asked them to develop a method such that the tissue will be immediately put in the formalin. The reason it was going into the sterile water was because the needle is very expensive and would need to be used again for the second core and obviously could not be rinsed in formalin. We have also considered asking them to use sterile saline to rinse the needle, but are not sure of the effect on the tissue. Does anyone have any ideas? We have had no problems until several weeks ago and nothing that we know of has changed. I have obviously watched timing, temperatures, used only fresh stains, alcohols, xylene, and all products in the processor. We use Richard Allen stains. We routinely use the following times but I am adding a xylene on each end and two more 100% ETOH for hydration and dehydration. Xylene-5 min Xylene--- 5 min 100% ETOH rinse 95 % ETOH rinse 80% ETOH rinse Distilled water for 1 min Hematoxylin 3 min Tap water rinse Clarifier-45 sec Tap water rinse Bluing---1 min Tap water rinse Eosin-20-30 sec 80% ETOH 95% ETOH 95% ETOH 100% ETOH 100% ETOH Xylene Xylene Thanks in advance for any help. Jane Jane Moose Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 From slappycraw <@t> yahoo.com Thu Aug 23 08:11:09 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Aug 23 08:11:14 2007 Subject: [Histonet] FW: faded H&E stain In-Reply-To: <8BB5FC36DDA373488E60177757BBD6980F298D@ncmhexchbe01.NewberryHospital.net> Message-ID: <136814.65250.qm@web53605.mail.re2.yahoo.com> Try staining those tissues seperately once just to see the difference, also I would use an 80% just before the eosin but not after. I might even suggest processing them on a seperate machine if possible, one with a shorter time frame than the larger tissues. "Jane C. Moose" wrote: Recently we have experienced sporadic problems with our routine H&E stains of some small GI biopsies and Ultrasound guided needle biopsies of breast tissue. The stains appear faded and almost unreadable. Other tissue processed at the same time, cut, oven dried, and stained in the same rack of slides (we hand stain) are stained appropriately. Usually these are from larger sections of tissue such as appendix, gallbladder, skin, uterus, colon segment, etc. This faded stain may happen one day and then not again for several and then happen again just for certain tissues that day. We are working with the "collecting techs" to ensure that the tissue is appropriately handled before we receive it. For breast tissue, the technique that has been used was to drop the biopsy in sterile water just to remove it from the syringe and transfer immediately to formalin. This has worked well for quite some time. Today however, we have asked them to develop a method such that the tissue will be immediately put in the formalin. The reason it was going into the sterile water was because the needle is very expensive and would need to be used again for the second core and obviously could not be rinsed in formalin. We have also considered asking them to use sterile saline to rinse the needle, but are not sure of the effect on the tissue. Does anyone have any ideas? We have had no problems until several weeks ago and nothing that we know of has changed. I have obviously watched timing, temperatures, used only fresh stains, alcohols, xylene, and all products in the processor. We use Richard Allen stains. We routinely use the following times but I am adding a xylene on each end and two more 100% ETOH for hydration and dehydration. Xylene-5 min Xylene--- 5 min 100% ETOH rinse 95 % ETOH rinse 80% ETOH rinse Distilled water for 1 min Hematoxylin 3 min Tap water rinse Clarifier-45 sec Tap water rinse Bluing---1 min Tap water rinse Eosin-20-30 sec 80% ETOH 95% ETOH 95% ETOH 100% ETOH 100% ETOH Xylene Xylene Thanks in advance for any help. Jane Jane Moose Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From asmith <@t> mail.barry.edu Thu Aug 23 08:12:38 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Aug 23 08:12:50 2007 Subject: [Histonet] Fw: Thiocarbohydrazide In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40F4@exchsrv01.barrynet.barry.edu> Easier said than done: the 4th edition of Pearse's HISTOCHEMISTRY has been out of print for years. It took me 3 years of watchful waiting on Barnes and Noble's used book site to get all 3 volumes. Total cost was well over $400. It would be really nice if someone (Krieger? Dover?) reprinted it. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Thursday, August 23, 2007 1:10 AM To: Agustin Chertcoff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fw: Thiocarbohydrazide The answer to your question is simple. Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless. Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results. John Kiernan Anatomy, UWO London, Canada --- > ----- Original Message ----- > From: Agustin Chertcoff > To: histonet@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 5:47 PM > Subject: Thiocarbohydrazide > > > Hi Histoneters! > I need a protocole for OTO Technics (Osmium tetroxide, > Thiocarbohydrazide) for Electron Microcoscopy, specially as > preparing the Thiocarbohidrazide solution and the correct > temperature > > Thanks in advanced ! > > Ht Agustin Victor Chertcoff > Electron Microscopy Service > National Institute of Microbiology C G Malbran > Buenos Aires > Argentina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From contact <@t> excaliburpathology.com Thu Aug 23 08:47:11 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Aug 23 08:47:17 2007 Subject: [Histonet] Faded H&E Stain Message-ID: <939657.18966.qm@web50112.mail.re2.yahoo.com> Describe your fading. Faint blue nuclei and eosin fine? Both stains faint? Are the tissues from post-radiation pts? Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From mike.collins <@t> imperial.ac.uk Thu Aug 23 09:58:48 2007 From: mike.collins <@t> imperial.ac.uk (Collins, Michael) Date: Thu Aug 23 09:59:03 2007 Subject: [Histonet] Retirement/Pension Message-ID: Congratulations Jacqui! I could benefit from some advice from British histonetters about missing pensions. I was a research assistant in the Dept. of Histopathology, Charing Cross & Westminster Medical School, Westminster site from 1984-1986 and paid 6% of my salary into the superannuation scheme under the Whitley Council regulations as specified in my contract. When my contract ended, I left the country for about 10 weeks, returned and did some teaching before becoming a research assistant in histopath at the Royal College of Surgeons and University College. When in later years I tried to claim my pension, no trace of my payments could be found. Westminster closed and the assets were transferred to Charing Cross before the Chelsea & Westminster Hospital opened. I've got more than 30 years of histology under my belt! Thanks in advance, Mike. From eroux <@t> medimabs.com Thu Aug 23 10:08:30 2007 From: eroux <@t> medimabs.com (Emmanuelle Roux) Date: Thu Aug 23 10:09:34 2007 Subject: [Histonet] inhibitory mouse monoclonal antibody to TNF Message-ID: <20070823150928.49C7EBF@mail.business.allstream.net> Hi everyone, Does anyone know where I could find a good inhibitory mouse monoclonal antibody to TNF? Thank you for your help, Emmanuelle Emmanuelle Roux, Ph.D. From ttruscot <@t> vetmed.wsu.edu Thu Aug 23 10:26:29 2007 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Aug 23 10:26:36 2007 Subject: [Histonet] FW: faded H&E stain In-Reply-To: <8BB5FC36DDA373488E60177757BBD6980F298D@ncmhexchbe01.NewberryHospital.net> References: <8BB5FC36DDA373488E60177757BBD6980F298D@ncmhexchbe01.NewberryHospital.net> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44CB6BD17@cvm36.vetmed.wsu.edu> Hi Jane, Since other tissues in the same processing run and same staining run turn out OK, I would look at the differences in handling the small biopsies. Are the biopsies put in special cassettes or on sponges that are preventing fluid exchange anywhere along the way? Since the problem is sporadic, maybe their position in the processor makes a difference- are they on the bottom or top of the chamber? Specimens on top might trap air in their cassettes and prevent fluid exchange. Tom Truscott USDA-ADRU Pullman, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Thursday, August 23, 2007 5:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: faded H&E stain Recently we have experienced sporadic problems with our routine H&E stains of some small GI biopsies and Ultrasound guided needle biopsies of breast tissue. The stains appear faded and almost unreadable. Other tissue processed at the same time, cut, oven dried, and stained in the same rack of slides (we hand stain) are stained appropriately. Usually these are from larger sections of tissue such as appendix, gallbladder, skin, uterus, colon segment, etc. This faded stain may happen one day and then not again for several and then happen again just for certain tissues that day. We are working with the "collecting techs" to ensure that the tissue is appropriately handled before we receive it. For breast tissue, the technique that has been used was to drop the biopsy in sterile water just to remove it from the syringe and transfer immediately to formalin. This has worked well for quite some time. Today however, we have asked them to develop a method such that the tissue will be immediately put in the formalin. The reason it was going into the sterile water was because the needle is very expensive and would need to be used again for the second core and obviously could not be rinsed in formalin. We have also considered asking them to use sterile saline to rinse the needle, but are not sure of the effect on the tissue. Does anyone have any ideas? We have had no problems until several weeks ago and nothing that we know of has changed. I have obviously watched timing, temperatures, used only fresh stains, alcohols, xylene, and all products in the processor. We use Richard Allen stains. We routinely use the following times but I am adding a xylene on each end and two more 100% ETOH for hydration and dehydration. Xylene-5 min Xylene--- 5 min 100% ETOH rinse 95 % ETOH rinse 80% ETOH rinse Distilled water for 1 min Hematoxylin 3 min Tap water rinse Clarifier-45 sec Tap water rinse Bluing---1 min Tap water rinse Eosin-20-30 sec 80% ETOH 95% ETOH 95% ETOH 100% ETOH 100% ETOH Xylene Xylene Thanks in advance for any help. Jane Jane Moose Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Thu Aug 23 10:48:10 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Thu Aug 23 10:48:15 2007 Subject: [Histonet] Human ad Mouse Smoothened Abs for IHC Message-ID: <35e16a770708230848j3b96d986y676fa2b47ae039be@mail.gmail.com> Dear Histonetters! Has anyone ever worked with Smoothened (7 transmembrane protein)? I am looking for antibodies to detect Mouse and/or Human Smoothened using IHC and Immunofluorescence.If anyone knows which antibodies work could they please share. It would be greatly appreciated. Thank you in advance. Sincerely, Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA. From tjasper <@t> copc.net Thu Aug 23 11:27:41 2007 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Aug 23 11:28:58 2007 Subject: FW: [Histonet] Histology Position Message-ID: <90354A475B420441B2A0396E5008D4965E1F64@copc-sbs.COPC.local> I posted this position on Tuesday 8-21-07 - please note there has been a change to the fax number. It is still... Attn. Pam Sylvester - new fax # (541)693-2648 Thank you, Thomas Jasper -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, August 21, 2007 5:17 PM To: histonet@lists.utsouthwestern.edu Cc: Pam Sylvester Subject: [Histonet] Histology Position Central Oregon Regional Pathology Services, Bend, OR is seeking an HT or HTL, ASCP registered or registry eligible preferred. Full-time position with rotating weekend coverage. Responsibilities include embedding, microtomy, routine staining, automated immunohistochemistry and special staining. Excellent benefits, including health insurance and retirement plan and competitive salary. Bend is located in central Oregon at the base of the Cascade Mountains. Hiking, backpacking, rock climbing, fishing and skiing opportunities abound. Central Oregon Regional Pathology Services, 1348 NE Cushing Dr., Bend, OR 97701 Attn: Pam Sylvester or fax resume to: (541) 389-5723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From agustinvictor <@t> gmail.com Thu Aug 23 11:46:51 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Thu Aug 23 11:46:51 2007 Subject: [Histonet] Fw: Thiocarbohydrazide References: <004d01c7e500$e093ece0$590215ac@Pentium4> Message-ID: <002601c7e5a5$3763cb40$590215ac@Pentium4> Hi histoneters! Sorry, you maybe don't understand for my bad expression of the language.I don't ask so that it is used. here are not books Pearse current. I will not invest money of my pocket for this stain. I need the simple protocol. I not wanted to buy a book,thanks! I need this protocol,somebody that has made this reaction for the histochemical and ultracytochemical demonstration of glycomacromolecules for electron microscopy TCH was introduced to cytochemistry, in the first adaptation,of the PAS reaction, to demonstrate tissue glycomacromolecules for TEM. The reaction depends upon selective oxidation of vicinalor 1,2-glycols, ethanolamines, a-hydroxyaldehydes, and ahydroxy-ketones.Enhancement of fine structure of cytomembranes by the osmium-thiocarbohydrazide-osmium (OTO) bridging. the OTO reaction as a simpler alternative to evaporative metal coating for SEM. Excuse me if I didn't understand thanks to all! Ht Agustin Victor Chertcoff National Institute of Microbiology.Electron Microscopy Service Municipal Hospital of Gastroenterology Patology Service. Buenos Aires.Argentina.South America John Kiernan To: Agustin Chertcoff Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, August 23, 2007 2:09 AM Subject: Re: [Histonet] Fw: Thiocarbohydrazide The answer to your question is simple. Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless. Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results. John Kiernan Anatomy, UWO London, Canada --- > ----- Original Message ----- > From: Agustin Chertcoff > To: histonet@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 5:47 PM > Subject: Thiocarbohydrazide > > > Hi Histoneters! > I need a protocole for OTO Technics (Osmium tetroxide, > Thiocarbohydrazide) for Electron Microcoscopy, specially as > preparing the Thiocarbohidrazide solution and the correct > temperature > > Thanks in advanced ! > > Ht Agustin Victor Chertcoff > Electron Microscopy Service > National Institute of Microbiology C G Malbran > Buenos Aires > Argentina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Margaret.Perry <@t> sdstate.edu Thu Aug 23 12:03:49 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Aug 23 12:04:00 2007 Subject: [Histonet] looking for antibody for amyloid Message-ID: We are trying to find antibodies for Amyloid AA and Amyloid AL that will work in cats, dogs and livestock (veterinary diagnostic lab). Is there one antibody I can use for both or do I need to make a cocktail? What is your recommendation on what to use? We will also be running a Congo Red. I can find amyloid A for humans but have not had any luck searching for amyloid AL. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From ks_4foots <@t> yahoo.com Thu Aug 23 13:17:17 2007 From: ks_4foots <@t> yahoo.com (nevets yelkaoc) Date: Thu Aug 23 13:17:28 2007 Subject: [Histonet] Histology position wanted Message-ID: <396562.40353.qm@web44801.mail.sp1.yahoo.com> I'm looking for a histology position in South central Wisconsin or North central Illinois. I will also do PT, temp, or on-call. 15 years experiance in routine Histology and experiance in non-GYN prep work. Thanks, "Me" --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From Barry.R.Rittman <@t> uth.tmc.edu Thu Aug 23 13:23:24 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Aug 23 13:28:11 2007 Subject: [Histonet] Fw: Thiocarbohydrazide References: <004d01c7e500$e093ece0$590215ac@Pentium4> <002601c7e5a5$3763cb40$590215ac@Pentium4> Message-ID: Augustin There is nothing wrong with your English. Sorry that I do not have the protocol here.I believe that the point that John was trying to make and one with which I wholeheartedly concur is that this is a very dangerous chemical and you really need to not only have the protocol but understand the reaction and also exercise extreme care. This is for your safety and that of those also in your laboratory. Most protocols do not provide adequete safety instructions for preparation of solutions. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Agustin Chertcoff Sent: Thu 8/23/2007 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fw: Thiocarbohydrazide Hi histoneters! Sorry, you maybe don't understand for my bad expression of the language.I don't ask so that it is used. here are not books Pearse current. I will not invest money of my pocket for this stain. I need the simple protocol. I not wanted to buy a book,thanks! I need this protocol,somebody that has made this reaction for the histochemical and ultracytochemical demonstration of glycomacromolecules for electron microscopy TCH was introduced to cytochemistry, in the first adaptation,of the PAS reaction, to demonstrate tissue glycomacromolecules for TEM. The reaction depends upon selective oxidation of vicinalor 1,2-glycols, ethanolamines, a-hydroxyaldehydes, and ahydroxy-ketones.Enhancement of fine structure of cytomembranes by the osmium-thiocarbohydrazide-osmium (OTO) bridging. the OTO reaction as a simpler alternative to evaporative metal coating for SEM. Excuse me if I didn't understand thanks to all! Ht Agustin Victor Chertcoff National Institute of Microbiology.Electron Microscopy Service Municipal Hospital of Gastroenterology Patology Service. Buenos Aires.Argentina.South America John Kiernan To: Agustin Chertcoff Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, August 23, 2007 2:09 AM Subject: Re: [Histonet] Fw: Thiocarbohydrazide The answer to your question is simple. Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless. Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results. John Kiernan Anatomy, UWO London, Canada --- > ----- Original Message ----- > From: Agustin Chertcoff > To: histonet@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 5:47 PM > Subject: Thiocarbohydrazide > > > Hi Histoneters! > I need a protocole for OTO Technics (Osmium tetroxide, > Thiocarbohydrazide) for Electron Microcoscopy, specially as > preparing the Thiocarbohidrazide solution and the correct > temperature > > Thanks in advanced ! > > Ht Agustin Victor Chertcoff > Electron Microscopy Service > National Institute of Microbiology C G Malbran > Buenos Aires > Argentina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Thu Aug 23 14:52:05 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Aug 23 14:52:16 2007 Subject: [Histonet] HistoChoice? Message-ID: <238754.74195.qm@web52512.mail.re2.yahoo.com> Hello All! My Boss just handed me information on the fixative HistoChoice. Sounds good but is anyone out there using it? If so how do you feel about it? Thank you all in advance, Patricia Adams ____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469 From agustinvictor <@t> gmail.com Thu Aug 23 15:19:30 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Thu Aug 23 15:19:39 2007 Subject: [Histonet] Fw: Thiocarbohydrazide References: <004d01c7e500$e093ece0$590215ac@Pentium4> <002601c7e5a5$3763cb40$590215ac@Pentium4> Message-ID: <005501c7e5c2$ec0eaf70$590215ac@Pentium4> Thanks! I cannot interpret sometimes because it is not my language.Sorry We have the problem of the milk precipitate and crystallized when keeping the solution. thank you to prevent! we are prepared to make mortal solutions & know about the danger of this technique May be fatal if inhaled, swallowed, or absorbed through skin. May cause irritation. Use only in a chemical fume hood. Do not breathe dust. Avoid contact with eyes, skin, and clothing. Wear protective goggles and gloves when handling material. Wash immediately after handling. Keep container tightly closed. Store in a cool, dry place. Thanks to all! Agustin ----- Original Message ----- From: "Rittman, Barry R" To: "Agustin Chertcoff" ; Sent: Thursday, August 23, 2007 3:23 PM Subject: RE: [Histonet] Fw: Thiocarbohydrazide Augustin There is nothing wrong with your English. Sorry that I do not have the protocol here.I believe that the point that John was trying to make and one with which I wholeheartedly concur is that this is a very dangerous chemical and you really need to not only have the protocol but understand the reaction and also exercise extreme care. This is for your safety and that of those also in your laboratory. Most protocols do not provide adequete safety instructions for preparation of solutions. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Agustin Chertcoff Sent: Thu 8/23/2007 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fw: Thiocarbohydrazide Hi histoneters! Sorry, you maybe don't understand for my bad expression of the language.I don't ask so that it is used. here are not books Pearse current. I will not invest money of my pocket for this stain. I need the simple protocol. I not wanted to buy a book,thanks! I need this protocol,somebody that has made this reaction for the histochemical and ultracytochemical demonstration of glycomacromolecules for electron microscopy TCH was introduced to cytochemistry, in the first adaptation,of the PAS reaction, to demonstrate tissue glycomacromolecules for TEM. The reaction depends upon selective oxidation of vicinalor 1,2-glycols, ethanolamines, a-hydroxyaldehydes, and ahydroxy-ketones.Enhancement of fine structure of cytomembranes by the osmium-thiocarbohydrazide-osmium (OTO) bridging. the OTO reaction as a simpler alternative to evaporative metal coating for SEM. Excuse me if I didn't understand thanks to all! Ht Agustin Victor Chertcoff National Institute of Microbiology.Electron Microscopy Service Municipal Hospital of Gastroenterology Patology Service. Buenos Aires.Argentina.South America John Kiernan To: Agustin Chertcoff Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, August 23, 2007 2:09 AM Subject: Re: [Histonet] Fw: Thiocarbohydrazide The answer to your question is simple. Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless. Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results. John Kiernan Anatomy, UWO London, Canada --- > ----- Original Message ----- > From: Agustin Chertcoff > To: histonet@lists.utsouthwestern.edu > Sent: Monday, August 13, 2007 5:47 PM > Subject: Thiocarbohydrazide > > > Hi Histoneters! > I need a protocole for OTO Technics (Osmium tetroxide, > Thiocarbohydrazide) for Electron Microcoscopy, specially as > preparing the Thiocarbohidrazide solution and the correct > temperature > > Thanks in advanced ! > > Ht Agustin Victor Chertcoff > Electron Microscopy Service > National Institute of Microbiology C G Malbran > Buenos Aires > Argentina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Aug 23 15:19:51 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Aug 23 15:20:19 2007 Subject: [Histonet] re: anti-human antibodies Message-ID: <005501c7e5c2$f5f9e680$4101a8c0@carlba65530bda> Well, I tried your Ab and have never got a positive on human cells in mouse FFPW sections +/- HIER I also tried Serotec's anti HNuc and got a better result. However, my best , consistent result was using an anti human mitochondrial Ab. I hope that here http://www.immunoportal.com/index.php in the Image gallery , are some pics that you may care to comment on. Best wishes Carl From Reuel.Cornelia <@t> tsrh.org Thu Aug 23 16:48:54 2007 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Aug 23 16:49:32 2007 Subject: [Histonet] MMP 13 and Collagen X Message-ID: <46CDBA76020000C500019DEB@nwcl02.tsrh.org> Is there any of you have done MMP 13 and Collagen X on a rat tibial tissue on paraffin sections. Who will be your recommended distributor or manufacturer. What is your dilution. Would you send me a protocol. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From asachau <@t> titanmed.com Thu Aug 23 16:53:11 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Thu Aug 23 16:55:07 2007 Subject: [Histonet] (no subject) In-Reply-To: <46CDBA76020000C500019DEB@nwcl02.tsrh.org> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7BA3012@titansbs1.corp.titanmed.com> Hello! I have a couple positions available in the Midwest for Histologists (ASCP) in the same hospital. Married couples or friends that would like traveling assignments together also welcome. Start date ASAP, housing, travel, car paid. Please contact me if you are interested! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From AGrobe2555 <@t> aol.com Thu Aug 23 17:01:12 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Aug 23 17:01:37 2007 Subject: [Histonet] Re:Histochoice Message-ID: We use it and have used it for some time. It seems to work fairly well, though none of the samples we do are human patients. We are exclusively a research lab. The nice thing is that it doesn't seem to require antigen retrieval to the extent of formalin-fixed tissues. However, in light of samples I have worked with lately, I am finding that some IHC doesn't seem to work as well after long-term storage (~5 years). As to staining (H&E), there seems to be very little difference in intensity and has not affected our IHC. All that said, I have not done a side-by-side comparison on the same tissues fixed with Histochoice vs Formalin, though I do have some in the wings to do this eventually. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From peddinti_2002us <@t> yahoo.co.in Thu Aug 23 17:18:50 2007 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Thu Aug 23 17:19:04 2007 Subject: [Histonet] (no subject) Message-ID: <854334.68828.qm@web8708.mail.in.yahoo.com> OOh ya , We wrap it in whatman filter paper no"0" it'll give better results Regards, Peddinti --------------------------------- Try the revolutionary next-gen Yahoo! Mail. Click here. From koellingr <@t> comcast.net Fri Aug 24 00:48:51 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Aug 24 00:49:03 2007 Subject: [Histonet] inhibitory mouse monoclonal antibody to TNF Message-ID: <082420070548.19339.46CE71430002A17F00004B8B22007623029D09020704040A0105@comcast.net> Emmanuelle, Have done a lot of work with anti-TNF antibodies. Are you asking for a mouse monoclonal that sequesters TNF in-vivo or a classical neutralizing (blocking) antibody? What are you trying to do? In-vitro, in-vivo, IHC, Westerns? Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Emmanuelle Roux" > Hi everyone, > > > > Does anyone know where I could find a good inhibitory mouse monoclonal > antibody to TNF? > > Thank you for your help, > > > > Emmanuelle > > > > Emmanuelle Roux, Ph.D. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgreen <@t> hsh.org Fri Aug 24 08:24:03 2007 From: kgreen <@t> hsh.org (Green, Kathy) Date: Fri Aug 24 08:24:17 2007 Subject: [Histonet] PAS Fungus stain Message-ID: We used to do a PAS fungus by hand and I can't seem to find the protocol anywhere. Someone ran off with one of our books. Anyway I'm looking for the procedure to do the PAS Fungus (we use Ventana for now). I remember it used Periodic acid - ? %, Schiff's, light green, etc. Does anyone have the "receipe"? Thanks Kathy Green, HT (ASCP) Lead Tech Histology Dept. Holy Spirit Hospital 503 N 21st Street Camp Hill, Pa 17011 E-mail: kgreen@hsh.org Phone: 717-763-2930 Blackberry: 717-370-1726 Fax: 717-763-2947 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From rjbuesa <@t> yahoo.com Fri Aug 24 08:36:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 24 08:37:05 2007 Subject: [Histonet] PAS Fungus stain In-Reply-To: Message-ID: <662150.12185.qm@web61225.mail.yahoo.com> Kathy: Follow the same protocol as for PAS glycogen. Don't have that either? Please let me know to send it to you. Ren? J. "Green, Kathy" wrote: We used to do a PAS fungus by hand and I can't seem to find the protocol anywhere. Someone ran off with one of our books. Anyway I'm looking for the procedure to do the PAS Fungus (we use Ventana for now). I remember it used Periodic acid - ? %, Schiff's, light green, etc. Does anyone have the "receipe"? Thanks Kathy Green, HT (ASCP) Lead Tech Histology Dept. Holy Spirit Hospital 503 N 21st Street Camp Hill, Pa 17011 E-mail: kgreen@hsh.org Phone: 717-763-2930 Blackberry: 717-370-1726 Fax: 717-763-2947 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From jfrank <@t> med.unc.edu Fri Aug 24 09:25:32 2007 From: jfrank <@t> med.unc.edu (Jonathan Frank) Date: Fri Aug 24 09:26:37 2007 Subject: [Histonet] BrdU/Doublecortin double labeling problem Message-ID: <200708241426.l7OEQ9br014226@smtp-mx.med.unc.edu> Good morning. I am attempting to double label Brain tissue with BrdU (Abcam 1893 raised in sheep) and Doublecortin (C-18 Santa Cruz raised in goat) and I am having some troubles that you may have suggestions to. The brains are perfused with 4% paraformaldehyde and post fixed overnight at 4oC. The brains are then cryoprotected in 30% sucrose and cut on a cryostat at 20um. I am using Alexa fluor 594 donkey-anti sheep and 488 donkey-anti goat for my fluorescent secondary antibodies. I have had good success using each primary/secondary individually and the positive and negative controls seem to act as expected. The labeling also occurs in the correct part of the cell individually. The problem arises when I do the double labeling step where any BrdU positive cell is also doublecortin positive (not expected) and the morphology and points of intensity are exactly the same. I have tried this on tissues where Doublecortin should not be expressed and come up with the same problem. Here is the protocol I have been using: Blocking buffer = 8% donkey serum, 0.3% Bovine serum albumin, 0.3% Triton X-100, in 1x PBS Washing buffer = 0.3% Bovine serum albumin, 0.3% Triton X-100 in 1x PBS 1. 10mm Citrate buffer (pH 6) for 30' at 95oC 2. Allow to cool for 10' 3. 2 x 5' wash in 1x PBS 4. Block 1 hour 5. Doublecortin 1o AB (1:400 in washing buffer) overnight at 4oC 6. Wash 2 x 5' in washing buffer 7. 2N HCl 20' at RT 8. 2 x 5' wash in Tris Buffer (ph 7.6) 9. BrdU 1o AB (1:1500 in washing buffer) overnight at RT 10. 4 x 5' wash in washing buffer 11. Doublecortin 2o AB 1hr 12. Wash 2 x 5' in washing buffer 13. BrdU 2o AB 1hr 14. Wash 2 x 5' in Washing buffer 15. Wash 2 x 5' 1x PBS 16. Alcohols to Xylenes and coverslip Has anyone encountered these problems or have suggestions as to how I may solve this problem? Thanks in advance Jon Frank Jon Frank Manager, Small Animal Resuscitation Research Lab Department of Emergency Medicine UNC-Chapel Hill jfrank@med.unc.edu 919-966-6442/919-966-7998 From MMargiotta <@t> bmhmc.org Fri Aug 24 10:43:13 2007 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Fri Aug 24 10:43:24 2007 Subject: [Histonet] Emergency plan Message-ID: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From mcauliff <@t> umdnj.edu Fri Aug 24 10:45:51 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 24 10:47:16 2007 Subject: [Histonet] mouse histology textbook In-Reply-To: References: Message-ID: <46CEFD2F.3020109@umdnj.edu> Hi Jacqui: Most histology texts deal with histology with at most one chapter on how tissues are prepared. If you want to know about staining you need a book on microtechnique or histological technique. It sounds like you need both books. Quite frankly, it sounds like you need to find an expert to consult with, you will be way ahead in time and money in the long run. Geoff Jacqui Detmar wrote: > Hi all. I am posting this message for a friend that works on mouse imaging here at Toronto. Here is her message: > > MICe (the institute she works in) wants to buy a histology textbook to help us "figure some stuff out". We just want basic info on histology, info on which stains are best for which tissues, and hopefully some detail on histological stains for the brain. > > I'm pretty sure they would like something solely for mice, but a general histology book would probably do well, too. Thanks! > > Jacqui Detmar > Samuel Lunenfeld Research Institute, > 600 University Avenue > Toronto, Ontario, Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Fri Aug 24 10:56:07 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 24 10:56:19 2007 Subject: [Histonet] Emergency plan In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> Message-ID: <336681.43945.qm@web61219.mail.yahoo.com> Michele: I used a large container filled with paraffin, placed all the processed cassettes inside and let it solidify. When the blocks were going to be prepared, I melted the whole block of solidified paraffin, and prepared the blocks. Ren? J. "Margiotta, Michele" wrote: Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From Jackie.O'Connor <@t> abbott.com Fri Aug 24 10:56:49 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Aug 24 10:57:47 2007 Subject: [Histonet] Emergency plan In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> Message-ID: Let them cool down, then they can be warmed up to embedding temp when needed. "Margiotta, Michele" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/24/2007 10:43 AM To cc Subject [Histonet] Emergency plan Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HoustonR <@t> chi.osu.edu Fri Aug 24 11:06:11 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Fri Aug 24 11:06:42 2007 Subject: [Histonet] platelet EM Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2119D693E@chi2k3ms01.columbuschildrens.net> Does anyone have a procedure for preparing platelets for EM examination? Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mhanna <@t> histosearch.com Fri Aug 24 12:08:30 2007 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Fri Aug 24 12:08:47 2007 Subject: [Histonet] Histology Job Openings Message-ID: <59F68E4A-AD3C-4B57-9624-36633F6C150F@histosearch.com> Hi Histonetters, These are the latest job openings listed on Histosearch: Product Support Specialist, Genetix, Newcastle, United Kingdom Histotechnologist, Ephrata Community Hospital, Ephrata, Pennsylvania Histotech, Dermatology Lab, Sonoma County, California Histology Technician III, University of Georgia, Athens, Georgia Histology Associate, Abbott Laboratories, North Chicago, Illinois Histotech, Peninsula Pathology Institute, Solotna, Alaska Histology Technician, Pfizer, San Diego, California Histotechnologist (HTL), University of Minnesota Medical Center, Minneapolis, Minnesota Histologist, Molecular Pathology Laboratory, Maryville, Tennessee There are over 100 job seekers registered on the site. Job postings are free. Best Regards, Marvin Hanna www.histosearch.com jobs.histosearch.com www.histoauctions.com From carl.hobbs <@t> kcl.ac.uk Fri Aug 24 12:20:19 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Aug 24 12:20:50 2007 Subject: [Histonet] Re: BrdU/Doublecortin double labeling problem Message-ID: <007701c7e673$0bc6e480$4101a8c0@carlba65530bda> Without thinking very hard, I would like to mention that general anti sheep and anti goat Abs will cross-react. Eg: If I have a sheep primary Ab, I use rabbit anti goat secondary as I have so few sheep primaries but many more goat primaries. Carl From vazquezr <@t> ohsu.edu Fri Aug 24 13:11:53 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Aug 24 13:12:33 2007 Subject: [Histonet] Emergency plan Message-ID: Michele, Take them out of the processor and let them solidify on a paper towel on the counter. When ready to embed, put them in the heated chamber and let them sit for awhile until melted again. Robyn >>> "Margiotta, Michele" 8/24/2007 8:43 AM >>> Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Aug 24 13:15:20 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Aug 24 13:15:52 2007 Subject: [Histonet] Emergency plan In-Reply-To: References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32047B5F9E@sjhaexc02.sjha.org> My method too... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, August 24, 2007 1:12 PM To: MMargiotta@bmhmc.org; histonet@pathology.swmed.edu Subject: Re: [Histonet] Emergency plan Michele, Take them out of the processor and let them solidify on a paper towel on the counter. When ready to embed, put them in the heated chamber and let them sit for awhile until melted again. Robyn >>> "Margiotta, Michele" 8/24/2007 8:43 AM >>> Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From philip.oshel <@t> cmich.edu Fri Aug 24 13:22:27 2007 From: philip.oshel <@t> cmich.edu (Philip Oshel) Date: Fri Aug 24 13:22:43 2007 Subject: [Histonet] platelet EM Message-ID: TEM or SEM? Fully spread platelets can be done as whole mounts by either method. SEM requires low kV accelerating voltages, less than 3 kV, 1.5 or lower is better (platelets are basically invisible in the SEM at 10 kV and above, the beam goes right through them). Ralph Albrecht et al in the 80s and 90s published several articles on platelets using SEM and TEM. I'd look up his papers/book chapters. Phil >Does anyone have a procedure for preparing platelets for EM examination? > > Thanks > Ronnie > > Ronnie Houston, MS, HT(ASCP)QIHC > Anatomic Pathology Manager > Columbus Children's Hospital > 700 Children's Drive > Columbus, OH 43205 > (614) 722 5465 > houstonr@chi.osu.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From Jackie.O'Connor <@t> abbott.com Fri Aug 24 13:22:41 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Aug 24 13:23:32 2007 Subject: [Histonet] Histology Job Opening In-Reply-To: Message-ID: We have a position open for a histology technician in our toxpath lab here at Abbott. If you would like to apply, please apply at www.Abbott.com. The job is posted as an R+D Tech 1 - and the requisition number is 46158BR. This is a fantastic opportunity for someone to get involved with a great organization. Jackie O'Connor, HT(ASCP)QIHC Histology Laboratory Supervisor Abbott Laboratories 100 Abbott Park Road Abbott Park, IL 60064 From LWatzek <@t> brch.com Fri Aug 24 13:44:53 2007 From: LWatzek <@t> brch.com (Laura Watzek) Date: Fri Aug 24 13:45:05 2007 Subject: [Histonet] (no subject) Message-ID: Does anyone monitor technical issues prohibiting a Pathologist rendering a frozen diagnosis? If so what are your parameters? Laura R. Watzek Pathology Supervisor Boca Raton Community Hospital 800 Meadows Road Boca Raton, FL 33486 (561) 955-4135 From Reuel.Cornelia <@t> tsrh.org Fri Aug 24 14:07:24 2007 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Aug 24 14:08:06 2007 Subject: Fwd: [Histonet] MMP 13 and Collagen X Message-ID: <46CDBA76020000C500019DEB@nwcl02.tsrh.org> Is there any of you have done MMP 13 and Collagen X immunocytochemistry on a rat tibial tissue on paraffin sections. Who will be your recommended distributor or manufacturer. What is your dilution. Can you please share to me your protocol. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From dw18 <@t> uchicago.edu Fri Aug 24 14:33:32 2007 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Fri Aug 24 14:33:44 2007 Subject: [Histonet] sheep/goat double labeling problem Message-ID: <20070824143332.AQK25418@m4500-03.uchicago.edu> Hi Jonathan & Histonet Apologies if you already got help here - I only read the digest and didn't see a reply. Your problem is not with "BrdU/Doublecortin double labeling " i.e. the epitopes but with the hosts the Abs were raised in - sheep and goat. These are evolutionarily very close together and so their IgGs have not diverged much in sequence. Consequently there is huge interspecies cross reaction between anti-goat or anti-sheep secondaries (which are polyclonal). So the secondary (say, anti-goat) against your second primary (made in goat) will also bind in large part to the first primary (made in sheep) - is that what you saw?. You could look for secondaries that have been adsorbed against the offending species, or you could use your existing reagents and just include serum of the first primary-host species (sheep, in the example) in with your second incubation secondary mix (the anti-goat step in my example). You will lose a lot of activity that way and will probably need to up your Ab concentration accordingly. But why don't you use more distinct hosts for your primaries? There are a plethora of anti-BrdU antibodies out there and you can surely find something different from either goat or your study species. If you are not working on a mouse tissues with high endogenous IgGs, I'd recommend the BD MAb 347850. It only needs a very simple denaturation procedure before the primary: Immerse the slides in 0.07 N NaOH for 2 minutes. Immerse the slides in PBS, pH 8.5, to neutralize the base. - none of that cooking in HCl and/or formamide for hours. The clone is B44 so you may find the same MAb from other suppliers too. -hope this helps David Wright Section of Neurosurgery University of Chicago ============================================== ORIGINAL MESSAGE Message: 14 Date: Fri, 24 Aug 2007 10:25:32 -0400 From: "Jonathan Frank" Subject: [Histonet] BrdU/Doublecortin double labeling problem To: histonet@lists.utsouthwestern.edu Message-ID: <200708241426.l7OEQ9br014226@smtp-mx.med.unc.edu> Content-Type: text/plain; charset=us-ascii Good morning. I am attempting to double label Brain tissue with BrdU (Abcam 1893 raised in sheep) and Doublecortin (C-18 Santa Cruz raised in goat) and I am having some troubles that you may have suggestions to. The brains are perfused with 4% paraformaldehyde and post fixed overnight at 4oC. The brains are then cryoprotected in 30% sucrose and cut on a cryostat at 20um. I am using Alexa fluor 594 donkey-anti sheep and 488 donkey-anti goat for my fluorescent secondary antibodies. I have had good success using each primary/secondary individually and the positive and negative controls seem to act as expected. The labeling also occurs in the correct part of the cell individually. The problem arises when I do the double labeling step where any BrdU positive cell is also doublecortin positive (not expected) and the morphology and points of intensity are exactly the same. I have tried this on tissues where Doublecortin should not be expressed and come up with the same problem. Here is the protocol I have been using: Blocking buffer = 8% donkey serum, 0.3% Bovine serum albumin, 0.3% Triton X-100, in 1x PBS Washing buffer = 0.3% Bovine serum albumin, 0.3% Triton X-100 in 1x PBS 1. 10mm Citrate buffer (pH 6) for 30' at 95oC 2. Allow to cool for 10' 3. 2 x 5' wash in 1x PBS 4. Block 1 hour 5. Doublecortin 1o AB (1:400 in washing buffer) overnight at 4oC 6. Wash 2 x 5' in washing buffer 7. 2N HCl 20' at RT 8. 2 x 5' wash in Tris Buffer (ph 7.6) 9. BrdU 1o AB (1:1500 in washing buffer) overnight at RT 10. 4 x 5' wash in washing buffer 11. Doublecortin 2o AB 1hr 12. Wash 2 x 5' in washing buffer 13. BrdU 2o AB 1hr 14. Wash 2 x 5' in Washing buffer 15. Wash 2 x 5' 1x PBS 16. Alcohols to Xylenes and coverslip Has anyone encountered these problems or have suggestions as to how I may solve this problem? Thanks in advance Jon Frank Jon Frank Manager, Small Animal Resuscitation Research Lab Department of Emergency Medicine UNC-Chapel Hill jfrank@med.unc.edu 919-966-6442/919-966-7998 From mcauliff <@t> umdnj.edu Fri Aug 24 14:46:58 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 24 14:47:49 2007 Subject: [Histonet] mouse spinal cord Message-ID: <46CF35B2.4010004@umdnj.edu> According to Biscoe et al. Quart. J. Exp. Physiol. 67:473-494, 1982, the mouse spinal cord has: 8 cervical nerves 13 thoracic nerves 6 lumbar nerves 4 sacral nerves Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Janci.Wellborn <@t> stlukes-stl.com Fri Aug 24 14:51:46 2007 From: Janci.Wellborn <@t> stlukes-stl.com (Wellborn, Janci R) Date: Fri Aug 24 14:52:00 2007 Subject: [Histonet] Emergency plan In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32047B5F9E@sjhaexc02.sjha.org> Message-ID: <42DC3293408E094499C68DDDD45CA08E35C929@W3CEXCHANGE2.slh.stlukes.com> Ours too! Janci R Wellborn, BS, BSeD, HTL (ASCP) Anatomic Pathology Supervisor St. Luke's Hospital 232 South Woods Mill Road Chesterfield, Missouri 63017 (314) 205-6228 janci.wellborn@stlukes-stl.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, August 24, 2007 1:15 PM To: Robyn Vazquez; MMargiotta@bmhmc.org; histonet@pathology.swmed.edu Subject: RE: [Histonet] Emergency plan My method too... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, August 24, 2007 1:12 PM To: MMargiotta@bmhmc.org; histonet@pathology.swmed.edu Subject: Re: [Histonet] Emergency plan Michele, Take them out of the processor and let them solidify on a paper towel on the counter. When ready to embed, put them in the heated chamber and let them sit for awhile until melted again. Robyn >>> "Margiotta, Michele" 8/24/2007 8:43 AM >>> Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of St. Luke's Hospital. From turkekul <@t> gmail.com Fri Aug 24 21:23:35 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Aug 24 21:25:01 2007 Subject: [Histonet] BrDU/Doublecortin double IF Message-ID: Hi Jonathan, When you do double staining you should try the following. 1. Change the order of primaries try first BrdU and second doublecortin. 2. You can also try adding two primaries together (simultaneous staining) and also two secondaries together. 3. After each staining quickly check the slides under the microscope for any signal without mounting them. If you have signal proceed with the second staining. 4. After you do the pretreatment for the second staining also quickly check the slides If the signal from the first staining is still there. In most cases retrieval of one staining interferes with the second epitope. If pretreatment of one antibody masks the other try to optimize one of the antibodies with a pretreatment that does not affect the other or use antibodies from different species/vendor/clone. 5. For double staining try to use secondary antibodies that are design for multiple staining. They are re adsorbed for cross reactivity with other species. Sometimes they work. 6. Finally NEVER dehydrate and clear immunofluorescence slides with alcohols and xylene. You should mount the IF slides with glycerol based anti-fade media and keep the slides at -20 C horizontally in a slide folder. I use 0.1 % para-phenylenediamine in 80% glycerol in PBS for mounting. There are also different types of commercially available anti-fade mounting media for IF slides. Good Luck!!! Mesruh Turkekul MSKCC.org New York, NY 10021 From SCSCMohsSurgery <@t> aol.com Sat Aug 25 01:52:44 2007 From: SCSCMohsSurgery <@t> aol.com (SCSCMohsSurgery@aol.com) Date: Sat Aug 25 01:52:58 2007 Subject: [Histonet] Skin Cancer Surgery Center Message-ID: Hi Everyone, I was wondering if any of you fine folks are doing Mohs in an Ambulatory Surgery Center setting. I would love to chat with you. I am currently looking to build an ASC. Any information or other contact information would be greatly appreciated. Best Regards to you All. David Clark - returning Histonetter Portland, Oregon ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From carl.hobbs <@t> kcl.ac.uk Sat Aug 25 14:11:10 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Aug 25 14:11:49 2007 Subject: [Histonet] IF staining: can I mount pwax sections in DPX? Message-ID: <005e01c7e74b$b25d4950$4101a8c0@carlba65530bda> Hi. I usually carry out single/double IF-staining on pwax sections and coverslip sections in an aq. anti-fade mountant. This works well. However, can these sections be mounted in a solvent-based mountant and not only retain IF but also be able to retard fading? ( eg: DPX, after dehydrating in alcohol, clearing and mounting in xylene) Carl From carl.hobbs <@t> kcl.ac.uk Sat Aug 25 14:15:34 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Aug 25 14:16:15 2007 Subject: [Histonet] IF-fading retardants Message-ID: <006201c7e74c$4fd2bc10$4101a8c0@carlba65530bda> Hi. I would be grateful for opinions regarding mounting media for preserving/retarding fading of fluorescence in cells, sections on conventional slides. Carl From HornHV <@t> archildrens.org Sun Aug 26 09:28:18 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Sun Aug 26 09:28:47 2007 Subject: [Histonet] Emergency plan In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> References: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB72F3@EMAIL.archildrens.org> Put all the cassettes into a large container and fill it with paraffin and let it solidify. It would be just like embedding them but instead you have one very large block!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Friday, August 24, 2007 10:43 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Emergency plan Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From kemlo <@t> f2s.com Sun Aug 26 11:03:35 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sun Aug 26 11:04:29 2007 Subject: [Histonet] Emergency plan In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB72F3@EMAIL.archildrens.org> References: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB72F3@EMAIL.archildrens.org> Message-ID: Just let the cassettes solidify, why put in an extra step? What's Slot 820? Kemlo Rogerson Lead AHP Weston Area Health Authority -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: 26 August 2007 15:28 To: Margiotta, Michele; histonet@pathology.swmed.edu Subject: RE: [Histonet] Emergency plan Put all the cassettes into a large container and fill it with paraffin and let it solidify. It would be just like embedding them but instead you have one very large block!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Friday, August 24, 2007 10:43 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Emergency plan Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Sun Aug 26 19:52:23 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Sun Aug 26 19:53:03 2007 Subject: [Histonet] Emergency plan References: <922CE5B88F398948B4076A9A4340E7AF03411B22@bmh_exchange.bmhmc.org> Message-ID: <001e01c7e844$88451f40$a20b80cb@PDualCore> Michele, Take cassettes out of the processor and let them solidify on the counter. When ready to embed, put them in the heated chamber and let them sit for awhile until melted again. Muhammad Tahseen. ----- Original Message ----- From: "Margiotta, Michele" To: Sent: Friday, August 24, 2007 8:43 PM Subject: [Histonet] Emergency plan Hi All, I need to write up an emergency plan for the tasks that need to be taken care of during an emergency. If for some reason the tissue could not be embedded after processing, what would be a good holding pattern for the cassettes? Thanks for your help! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon Aug 27 03:30:11 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Aug 27 03:30:28 2007 Subject: [Histonet] histomorphometry Message-ID: Hi all, I have just been tasked with making some measurements of bony ingrowth into hydroxyapatite scaffolds. The area of interest is a hemispherical concavity moulded into the HA. I would like to get into contact with someone who could advise me on the best type/number/repetitions of measurement, and how the subsequent data could be reperesented. Any thoughts? It would be much appreciated as I have no experience in this field at all best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From louise.renton <@t> gmail.com Mon Aug 27 04:18:58 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Aug 27 04:19:09 2007 Subject: [Histonet] buffer solutions Message-ID: Hi all, another laboratory controversy reigns! When making up a buffer solution from salts what is the best practice: To measure out the dry salt, place in beaker and then add the required volume of water (ie up to 1liter) OR Measure out the 1liter and add the salts to that? ( I am asking in the context of making a 10x buffer soln, where the solute weighs up to 150g) Is there in fact any difference?? Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From jhr1x <@t> clinmed.gla.ac.uk Mon Aug 27 05:41:52 2007 From: jhr1x <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Mon Aug 27 05:42:06 2007 Subject: [Histonet] Buffer solutions Message-ID: <001301c7e896$e19d0150$1c7ed182@ssd4.gla.ac.uk> Hello Louise Add salts and dissolve in approximately 750 ml of water, pH and them make your solution to 1 L. Jim Reilly From wim.vandenbroeck <@t> UGent.be Mon Aug 27 07:34:20 2007 From: wim.vandenbroeck <@t> UGent.be (Prof. dr. Wim Van den Broeck) Date: Mon Aug 27 07:34:46 2007 Subject: [Histonet] Leica EM Tissue Processor Message-ID: <004c01c7e8a6$982e8830$58b3c19d@PC20DI03> Dear Friends, I have an EM related question, and I hope you can help me. We have just installed the Leica EM tissue processor (TP), and I was wondering if programmes for the TP are available for processing animal tissues in Spurr's embedding medium. Thanks in advance. Kind regards, Wim. ------------------------------------------------------- Wim Van den Broeck, DVM, MSc, PhD Professor in Cytology and Histology Department of Morphology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck@UGent.be ------------------------------------------------------- Ps Please consider the environment before printing this email. Please add this line to your email to tell others. From rjbuesa <@t> yahoo.com Mon Aug 27 07:53:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 27 07:53:34 2007 Subject: [Histonet] histomorphometry In-Reply-To: Message-ID: <648829.66369.qm@web61215.mail.yahoo.com> Louise: Number of measurements, in this as well as in any "sampling", will depend on the intrinsic variability of what you are measuring as determined by an initial sampling or series of measurements. If you are measuring a hemispherical cavity, you have to make sure that all your measurements correspond with the actual diameter, with the real hemisphere and not with a smaller fraction of the sphere, because if you mix them you will end with a extreme variability (deviation) that will require an unrealistic number of measurements. Once you determine the actual diameter, measure, lets say ten; then calculate average and standard deviation and with those 2 values, calculate sample size for the degree of precision you want to obtain (at least P<0.05 or P0.95). Hope this has not resultted too complex. I am sure that anybody in your institution with statistical background can "decipher" this e-mail. Ren? J. louise renton wrote: Hi all, I have just been tasked with making some measurements of bony ingrowth into hydroxyapatite scaffolds. The area of interest is a hemispherical concavity moulded into the HA. I would like to get into contact with someone who could advise me on the best type/number/repetitions of measurement, and how the subsequent data could be reperesented. Any thoughts? It would be much appreciated as I have no experience in this field at all best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From rjbuesa <@t> yahoo.com Mon Aug 27 07:56:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 27 07:56:35 2007 Subject: [Histonet] buffer solutions In-Reply-To: Message-ID: <835043.30382.qm@web61221.mail.yahoo.com> Louise: All pH buffer solutions are designated as Molar (or Normal) solutions, and by definiton a Molar (or Normal) solution (or fraction thereof) is prepared by wighing the solids and adding water UP TO a total volume of 1 litre. Ren? J. louise renton wrote: Hi all, another laboratory controversy reigns! When making up a buffer solution from salts what is the best practice: To measure out the dry salt, place in beaker and then add the required volume of water (ie up to 1liter) OR Measure out the 1liter and add the salts to that? ( I am asking in the context of making a 10x buffer soln, where the solute weighs up to 150g) Is there in fact any difference?? Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From ian.montgomery <@t> bio.gla.ac.uk Mon Aug 27 08:26:55 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Mon Aug 27 08:27:13 2007 Subject: FW: [Histonet] buffer solutions Message-ID: <001201c7e8ad$f01cbd30$6424d182@IBLS.GLA.AC.UK> Louise, Dissolve the salts completely then make up to 1 litre for a Molar solution. Dissolving the salts in 1 litre will give you a Molal solution. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 27 August 2007 10:19 To: Histonet@lists.utsouthwestern.edu; kevin Subject: [Histonet] buffer solutions Hi all, another laboratory controversy reigns! When making up a buffer solution from salts what is the best practice: To measure out the dry salt, place in beaker and then add the required volume of water (ie up to 1liter) OR Measure out the 1liter and add the salts to that? ( I am asking in the context of making a 10x buffer soln, where the solute weighs up to 150g) Is there in fact any difference?? Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Mon Aug 27 09:28:09 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Aug 27 09:29:27 2007 Subject: [Histonet] Region II Meeting in Delaware Coming Soon Message-ID: <6.2.5.6.2.20070827101644.01c31bd8@vet.upenn.edu> The Region II group has put together what we think is a very interesting and educational program for the meeting to be held on September 6,7,8, 2007 in Stanton Delaware (Just outside Wilmington DE and very close to Philadelphia, PA). We have 30 vendors (it contracted with all the mergers in companies this year). Speakers from local and out of state are coming to help us all advance our histology knowledge and careers. We have topics for clinical, research and academics to offer. Please go to pahisto.org for a full printable program with registration forms to assist you attend the workshops or seminars you are interested in taking. We have a special workshop on Saturday for animal IHC presented with Innovex. We hope this will help those of us (me included) with any issues we have with various species of animal work from rodents to veterinary specialities. Please join us and see what we can create as a group with histology education as our goal. If you can't make it to Denver for the National to get your CEUs join us at the Region II Meeting in Delaware!! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From KMH.02 <@t> ex.uchs.org Mon Aug 27 09:34:08 2007 From: KMH.02 <@t> ex.uchs.org (Hopkins, Karen) Date: Mon Aug 27 09:36:52 2007 Subject: [Histonet] Pinning specimens Message-ID: <640B23A5DC4B234BB065E56F2DB30596028AB66A@uchex2ucmc.uchs.org> When we receive large specimens such as colons, our pathologist opens them to let them fix overnight. We used to have large specimen containers that had a piece of rubber glued to the bottom of the container so we could pin the specimens to hold them open for better fixation. Little by little our supply of containers with rubber have decreased and we are unable to find a replacement. Does anyone know of a vendor who sells these or can you share what you do to hold open specimens? Thanks in advance, Karen :-) From king.laurie <@t> marshfieldclinic.org Mon Aug 27 09:46:10 2007 From: king.laurie <@t> marshfieldclinic.org (king.laurie@marshfieldclinic.org) Date: Mon Aug 27 09:46:22 2007 Subject: [Histonet] Pinning specimens Message-ID: <27a2d01c7e8b9$02011e50$7205010a@mfldclinframe.org> Karen, One method, pour paraffin into several sizes of plastic containers (like Tupperware or cheaper versions). Just enough to pin a specimen onto, but leave room for formalin. Let them harden and pin specimens onto paraffin and cover with formalin. We used to wash and reuse several times. One annoying thing, the paraffin block or sheet would eventually loosen and float. They can be used that way by taking the paraffin out for pinning and placing it back in the container floating upside down. Laurie ------Original Message------ From: "Hopkins, Karen" Date: Mon Aug 27, 2007 -- 09:37:23 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Pinning specimens When we receive large specimens such as colons, our pathologist opens them to let them fix overnight. We used to have large specimen containers that had a piece of rubber glued to the bottom of the container so we could pin the specimens to hold them open for better fixation. Little by little our supply of containers with rubber have decreased and we are unable to find a replacement. Does anyone know of a vendor who sells these or can you share what you do to hold open specimens? Thanks in advance, Karen :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon Aug 27 09:46:24 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Aug 27 09:46:57 2007 Subject: [Histonet] Pinning specimens In-Reply-To: <640B23A5DC4B234BB065E56F2DB30596028AB66A@uchex2ucmc.uchs.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA2C8@sjhaexc02.sjha.org> let paraffin solidify in the bottom of a Rubbermaid type pan - you can pin to it in the pan, or chill and pop it out and use in a large container...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hopkins, Karen Sent: Monday, August 27, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pinning specimens When we receive large specimens such as colons, our pathologist opens them to let them fix overnight. We used to have large specimen containers that had a piece of rubber glued to the bottom of the container so we could pin the specimens to hold them open for better fixation. Little by little our supply of containers with rubber have decreased and we are unable to find a replacement. Does anyone know of a vendor who sells these or can you share what you do to hold open specimens? Thanks in advance, Karen :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From mcauliff <@t> umdnj.edu Mon Aug 27 09:49:44 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Aug 27 09:51:01 2007 Subject: [Histonet] Pinning specimens In-Reply-To: <640B23A5DC4B234BB065E56F2DB30596028AB66A@uchex2ucmc.uchs.org> References: <640B23A5DC4B234BB065E56F2DB30596028AB66A@uchex2ucmc.uchs.org> Message-ID: <46D2E488.4060304@umdnj.edu> Go to Home Depot or an auto supply store and buy some cork sheet. Pin the specimens to it, then put in fixative upside down. Geoff Hopkins, Karen wrote: > When we receive large specimens such as colons, our pathologist opens > them to let them fix overnight. We used to have large specimen > containers that had a piece of rubber glued to the bottom of the > container so we could pin the specimens to hold them open for better > fixation. Little by little our supply of containers with rubber have > decreased and we are unable to find a replacement. Does anyone know of > a vendor who sells these or can you share what you do to hold open > specimens? Thanks in advance, Karen :-) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mpence <@t> grhs.net Mon Aug 27 09:53:43 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Aug 27 09:54:01 2007 Subject: [Histonet] Pinning specimens In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA2C8@sjhaexc02.sjha.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6DB@IS-E2K3.grhs.net> This is a good place to recycle some of your paraffin from your tissue processor! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, August 27, 2007 9:46 AM To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pinning specimens let paraffin solidify in the bottom of a Rubbermaid type pan - you can pin to it in the pan, or chill and pop it out and use in a large container...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hopkins, Karen Sent: Monday, August 27, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pinning specimens When we receive large specimens such as colons, our pathologist opens them to let them fix overnight. We used to have large specimen containers that had a piece of rubber glued to the bottom of the container so we could pin the specimens to hold them open for better fixation. Little by little our supply of containers with rubber have decreased and we are unable to find a replacement. Does anyone know of a vendor who sells these or can you share what you do to hold open specimens? Thanks in advance, Karen :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From godsgalnow <@t> aol.com Mon Aug 27 09:58:18 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Aug 27 09:58:34 2007 Subject: [Histonet] douple and triple staining billing issues Message-ID: <8C9B6D1D637678F-41C-7BF5@mblk-r28.sysops.aol.com> Hey--- Anyone out there have any documentation on charging for 2 or 3 antibodies when doing double and triple staining for IHC? Thanks, Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From jnocito <@t> satx.rr.com Mon Aug 27 11:11:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Aug 27 11:11:16 2007 Subject: [Histonet] Pinning specimens References: <661949901A768E4F9CC16D8AF8F2838CA1C6DB@IS-E2K3.grhs.net> Message-ID: <003101c7e8c4$f7433780$d49eae18@yourxhtr8hvc4p> I get some string and duct tape. I cut 5-6 pieces off string about 6 inches long and then tape it in multiple places on the bottom of the container (this will prevent the paraffin from floating). Then I fill the container with used paraffin. See my point? Sorry, couldn't resist. JTT ----- Original Message ----- From: "Mike Pence" To: "Weems, Joyce" ; "Hopkins, Karen" ; Sent: Monday, August 27, 2007 9:53 AM Subject: RE: [Histonet] Pinning specimens This is a good place to recycle some of your paraffin from your tissue processor! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, August 27, 2007 9:46 AM To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pinning specimens let paraffin solidify in the bottom of a Rubbermaid type pan - you can pin to it in the pan, or chill and pop it out and use in a large container...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hopkins, Karen Sent: Monday, August 27, 2007 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pinning specimens When we receive large specimens such as colons, our pathologist opens them to let them fix overnight. We used to have large specimen containers that had a piece of rubber glued to the bottom of the container so we could pin the specimens to hold them open for better fixation. Little by little our supply of containers with rubber have decreased and we are unable to find a replacement. Does anyone know of a vendor who sells these or can you share what you do to hold open specimens? Thanks in advance, Karen :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Mon Aug 27 13:09:00 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Aug 27 13:13:05 2007 Subject: [Histonet] Re: Pinning Specimens Message-ID: Hi, Another way is to cut a piece of styrofoam (like you'd get in a cooler container for refrigerated supplies) to fit into your tupperware. Lay several sheets of paper towels over the foam, place your specimen on the paper and pin. The paper towels allow the formalin to wick between the foam and the tissue. You can float the specimen upside down in the formalin or use something heavy to weight it down. It's cheap and when the foam gets nasty you can toss it and replace with a new piece. Erin Martin From AGrobe2555 <@t> aol.com Mon Aug 27 13:29:55 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Aug 27 13:30:11 2007 Subject: [Histonet] IF-fading retardants Message-ID: Carl, I used to use "FluorSave" mounting medium from Calbiochem (Cat # 345789). This mounting medium "hardens" so the coverslip doesn't move and you don't have to seal around the edges with nail polish. It also kept the signal from fading. I still stored the slides horizontally at 4C or -20C. I wouldn't suggest try in to dehydrate through xylene and coverslipping, as I suspect you would kill your signal. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From slappycraw <@t> yahoo.com Mon Aug 27 14:47:20 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Aug 27 14:47:30 2007 Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse Message-ID: <398401.75792.qm@web53603.mail.re2.yahoo.com> Looking for antibodies for these targets on FFPE mouse. We've tried CD117,Tryptase and Chymase already. Thanks --------------------------------- Pinpoint customers who are looking for what you sell. From kimtournear <@t> yahoo.com Mon Aug 27 15:23:27 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Mon Aug 27 15:23:39 2007 Subject: [Histonet] Moh's question????? Message-ID: <697532.94484.qm@web50611.mail.re2.yahoo.com> Hi out there, I supervise a Moh's lab (private practice) and was wondering if any of you Moh's techs could tell me the minimum requirement as to how long Moh's frozen slides should be kept. I'm not CAP regulated, only CLIA and I think their minimum is 2 years....any help would be appreciated....thanks in advance Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From mtarango <@t> nvcancer.org Mon Aug 27 16:30:50 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Aug 27 16:31:25 2007 Subject: [Histonet] Myofibroblasts VS Fibrobalsts In-Reply-To: <35e16a770708201009n33a9df8dm1b1ae7dac212c619@mail.gmail.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6E0A@NVCIEXCH02.NVCI.org> Use an antibody against smooth muscle actin. You don't even need to do pretreatment with this antibody. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Monday, August 20, 2007 10:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Myofibroblasts VS Fibrobalsts Hello Histonetters! I was wondering does anyone know a good way to differentiate between human myofibroblasts and fibroblasts. Is there a specific stain or an antibody for IHC??? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge,MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From NMargaryan <@t> childrensmemorial.org Mon Aug 27 16:34:11 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Aug 27 16:33:07 2007 Subject: [Histonet] ISH Message-ID: Hi histonetters, I got a protocol for ISH for cells and there is a step, which looks very strange for me: After cytospin cells on glass slides let them air dry at RT for 60min. Is it not too long for the 60 min drying? Will cells remain on the glass in this case or there is a risk pop off cells from the glass? Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From mtarango <@t> nvcancer.org Mon Aug 27 16:39:05 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Aug 27 16:39:29 2007 Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse In-Reply-To: <398401.75792.qm@web53603.mail.re2.yahoo.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6E0B@NVCIEXCH02.NVCI.org> You could do a toluene blue for mast cells. Maybe you could stain for IgE too, but I really don't know for sure. If you want an antibody, I'd say C-kit (CD117) is the best...but you've already done that. You could use CD56 for natural killers. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Monday, August 27, 2007 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse Looking for antibodies for these targets on FFPE mouse. We've tried CD117,Tryptase and Chymase already. Thanks --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Norm.Burnham <@t> propathlab.com Mon Aug 27 17:34:21 2007 From: Norm.Burnham <@t> propathlab.com (Norm Burnham) Date: Mon Aug 27 17:34:35 2007 Subject: [Histonet] Histobath freezing medium Message-ID: Bob, I noted your comments about using acetone in a Histobath 2. Did you ever find a non-flammable alternative? Thanks and regards, Norm Burnham ____________________________________ Norm Burnham Operations Director ProPath - The Leader in Pathology Services(r) 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 Phone: 214.237.1602 Fax: 214.237.1802 Cell: 702.883.3290 Email: norm.burnham@propathlab.com To learn more about ProPath, please visit www.ProPathLab.com ________________________________ ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From talulahgosh <@t> gmail.com Tue Aug 28 00:00:29 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Aug 28 00:00:43 2007 Subject: [Histonet] mouse histology textbook In-Reply-To: <46CEFD2F.3020109@umdnj.edu> References: <46CEFD2F.3020109@umdnj.edu> Message-ID: This may not help with your histology, but there is a new project on the internet with mri scans of mice at http://mouseatlas.caltech.edu/ Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From mtarango <@t> nvcancer.org Tue Aug 28 02:20:39 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Aug 28 02:21:19 2007 Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE011B6E0B@NVCIEXCH02.NVCI.org> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6E13@NVCIEXCH02.NVCI.org> I forgot to mention CD68 dendritic cells. CD68 (KP-1 or ham-56) will stain macrophages and monocytes too. You kind of have to know what you're looking at. Dendritic cells can be from more than just one lineage, and CD68 might only pick up the myeloid-related ones. There is an antibody against follicular dendritic cells from cell marque too. I can't really think of just one marker for dendritic cells. You'd be better off using more than 1 marker and calling a dendritic cell a dendritic cell based of a panel of antibodies. Maybe immunofloresence would be best here. I personally like CD68 (ham56 clone) because you can kind of see the long projections that dendritic cells have with ham-56. KP-1 looks like spray paint and ham-56 looks like oil painting or something. It's kind of funny since they're both CD68. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: Tarango, Mark Sent: Monday, August 27, 2007 2:39 PM To: 'Larry Woody'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse You could do a toluene blue for mast cells. Maybe you could stain for IgE too, but I really don't know for sure. If you want an antibody, I'd say C-kit (CD117) is the best...but you've already done that. You could use CD56 for natural killers. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Monday, August 27, 2007 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse Looking for antibodies for these targets on FFPE mouse. We've tried CD117,Tryptase and Chymase already. Thanks --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Aug 28 03:31:43 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Aug 28 03:31:58 2007 Subject: [Histonet] platelet EM Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EBB2@wahtntex2.waht.swest.nhs.uk> When I did my MSc I look at Glutathione S Transferase in rbcs and platelets (god if I know why, but it seemed a good idea at the time). I harvested both cell types by floating out saline washed peripheral blood on hypaque at different concentrations. The different cell types 'floated' on hypaque that was a different densities; I can't remember what concentration of hypaque I used for platelets but if I can find the slate that I wrote my research on I'll let you know; the University probably now uses books in their Library. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; What lies behind us and what lies before us are small matters compared to what lies within us. --Ralph Waldo Emerson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Aug 28 03:39:06 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Aug 28 03:39:19 2007 Subject: [Histonet] buffer solutions Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EBB3@wahtntex2.waht.swest.nhs.uk> When making up a buffer solution from salts what is the best practice: To measure out the dry salt, place in beaker and then add the required volume of water (ie up to 1liter) OR Measure out the 1liter and add the salts to that? ( I am asking in the context of making a 10x buffer soln, where the solute weighs up to 150g) Is there in fact any difference?? Bit like do you add milk to tea, or tea to milk (we all know you add milk to tea, don't we?). You add acid to water, not water to acid and alcohol to water, not water to alcohol. I would have thought you should add water to buffer salts as if you do it the other way round (buffer to water) you would end up with a volume greater than you ought. For example if you have 100 mls of aqua dist and you add salts you end up with more than 100 mls. Put the salts in first then make up to 100 mls; makes sense? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; What lies behind us and what lies before us are small matters compared to what lies within us. --Ralph Waldo Emerson his e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From moran.elish <@t> gmail.com Tue Aug 28 04:34:13 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Tue Aug 28 04:34:27 2007 Subject: [Histonet] staining mast cells and eosinophils Message-ID: <4a722ef70708280234y368d84d6j6930b00e8cebc5ba@mail.gmail.com> Hello, I am very new at histology and immunohistochemistry, so please excuse the simplicity of my questions. I wish to stain parrafin-embedded slides (murine/human skin and lung) for mast cells and eosinophils. That is- I want to be able to detect both cells on one slide. Is there anyway to stain with toluidine blue and also counterstain with another dye for eosinophils? Alternatively- can i stain for toluidine blue to get mast cells, and then do (on the same slide) immunohistochemistry using an anti-MBP antibody to detect eosinophils? I guess its more of a general question- can one use a simple dye AND immunohistochemistry (with antibodies) on the same slide, or is there interference? I would be very glad to receive advice and protocols. Many thanks, Moran Elishmereni Department of Pharmacology School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem From kimtournear <@t> yahoo.com Tue Aug 28 08:07:01 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Aug 28 08:07:49 2007 Subject: [Histonet] re: Moh's slides Message-ID: <151480.76067.qm@web50606.mail.re2.yahoo.com> Thanks everyone for your input regarding the Moh's slides...looks like most labs are keeping them 10 yrs, so I guess I will too...better safe than sorry... Thanks again.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From coleman_manufacturing <@t> yahoo.com Tue Aug 28 09:24:53 2007 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Tue Aug 28 09:27:08 2007 Subject: [Histonet] Plastic microwave accessories & Fischer products Message-ID: <261855.77935.qm@web37605.mail.mud.yahoo.com> To all: We can supply all plastic and metal microwave accessories for any make of unit at a lower cost to you. Also, we are a certified distributor for Fischer Scientific offering all products at a lower cost to you. Please respond to this email, call, or fax. Regards, Justin Coleman Coleman Manufacturing & Design Winnsboro, SC 29180 Phone 803.633.2124 fax 803.635.9401 From ktuttle <@t> umm.edu Tue Aug 28 10:23:01 2007 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Aug 28 10:24:58 2007 Subject: [Histonet] acetone fixed frozen sections In-Reply-To: <261855.77935.qm@web37605.mail.mud.yahoo.com> References: <261855.77935.qm@web37605.mail.mud.yahoo.com> Message-ID: <46D40595.90CE.001A.3@umm.edu> Can acetone fixed frozen sections be air dried and stored at room temperature? How long are they good at room temperature? For IHC. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From dbpiontek <@t> hotmail.com Tue Aug 28 10:34:00 2007 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Tue Aug 28 10:34:49 2007 Subject: [Histonet] Periodic Acid Formalin Fixation Message-ID: Dear Histonetters: I am performing Periodic Acid Formalin fixation on liver and noticing sinusoid shrinkage, as well as some edge effect? This is in comparison to a matched tissue control in 10% NBF. My recipe requires fixation at 4C for 48 hours via 1 gram of periodic acid in 100 mls 10% NBF. Anyone else using this fixation method? Any suggestions or advice is greatly appreciated, Denise Bland-Piontek, HTL(ASCP)CTBS(AATB) NIBRI _________________________________________________________________ Messenger Caf? ? open for fun 24/7. Hot games, cool activities served daily. Visit now. http://cafemessenger.com?ocid=TXT_TAGLM_AugWLtagline From gu.lang <@t> gmx.at Tue Aug 28 10:49:57 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 28 10:50:57 2007 Subject: AW: [Histonet] ISH In-Reply-To: Message-ID: <000301c7e98b$16cb0bc0$6412a8c0@dielangs.at> I have got the recommendation from an experienced ISH-worker, that one should airdry cell-preparations up to one day. Usually the cells adhere better to the glass-surface, when they airdry longer. For ISH airdrying (aging of cells) is a kind of pretreatment, that is continiued by digesting. In the protocol of this worker: Airdry until the next day - fixation in icecold Methanol-acetic acid (3+1) until ISH-test. Hope this helps Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Margaryan, Naira Gesendet: Montag, 27. August 2007 23:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] ISH Hi histonetters, I got a protocol for ISH for cells and there is a step, which looks very strange for me: After cytospin cells on glass slides let them air dry at RT for 60min. Is it not too long for the 60 min drying? Will cells remain on the glass in this case or there is a risk pop off cells from the glass? Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Aug 28 11:06:37 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 28 11:07:22 2007 Subject: [Histonet] acetone fixed frozen sections In-Reply-To: <46D40595.90CE.001A.3@umm.edu> Message-ID: <100511.52743.qm@web61212.mail.yahoo.com> The antigens in any section (even FFPE) kept in the air will start to oxidize and the IHC reaction will weaken in noticeable way after just 1 week. Ren? J. Kimberly Tuttle wrote: Can acetone fixed frozen sections be air dried and stored at room temperature? How long are they good at room temperature? For IHC. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From ktuttle <@t> umm.edu Tue Aug 28 12:00:18 2007 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Aug 28 12:02:18 2007 Subject: [Histonet] PSCA: Prostate Stem Cell Antigen? In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222EBB2@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222EBB2@wahtntex2.waht.swest.nhs.uk> Message-ID: <46D41C62.90CE.001A.3@umm.edu> Does anyone know where I can order this antibody? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From HoustonR <@t> chi.osu.edu Tue Aug 28 12:18:22 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Aug 28 12:19:38 2007 Subject: [Histonet] PSCA: Prostate Stem Cell Antigen? In-Reply-To: <46D41C62.90CE.001A.3@umm.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2119D7702@chi2k3ms01.columbuschildrens.net> Invitrogen (formerly Zymed) www.invitrogen.com/pathology 800 955 6288 Cat# 18-7371 $302 for 0.5ml concentrate Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, August 28, 2007 1:00 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] PSCA: Prostate Stem Cell Antigen? Does anyone know where I can order this antibody? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From ks_4foots <@t> yahoo.com Tue Aug 28 12:29:48 2007 From: ks_4foots <@t> yahoo.com (nevets yelkaoc) Date: Tue Aug 28 12:31:03 2007 Subject: [Histonet] amyloid contol Message-ID: <780835.76095.qm@web44815.mail.sp1.yahoo.com> Looking for an amyloid contol. Our normal supplier is out. Even a few unstained amyloid positive controls would help. Steve --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From vazquezr <@t> ohsu.edu Tue Aug 28 12:45:29 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Aug 28 12:49:56 2007 Subject: [Histonet] Plastic microwave accessories & Fischer products Message-ID: Is this not blatant advertising? Or did someone inquire? Just curious. Robyn >>> "Coleman Manufacturing" 8/28/2007 7:24:53 AM >>> To all: We can supply all plastic and metal microwave accessories for any make of unit at a lower cost to you. Also, we are a certified distributor for Fischer Scientific offering all products at a lower cost to you. Please respond to this email, call, or fax. Regards, Justin Coleman Coleman Manufacturing & Design Winnsboro, SC 29180 Phone 803.633.2124 fax 803.635.9401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aneeshdhiman <@t> gmail.com Tue Aug 28 12:57:31 2007 From: aneeshdhiman <@t> gmail.com (aneesh Dhiman) Date: Tue Aug 28 12:58:23 2007 Subject: [Histonet] cell blocks Message-ID: <69ac8f280708281057u63f5492fpd79e2e2751f28393@mail.gmail.com> Hi Histonetters If a cytology specimen is prefixed in 50% Eth Alcohol. How do we proceed for Immno markers? what about the controls do they need to be alcohol fixed or Formalin fixed can be used? Aneesh Dhiman From mpence <@t> grhs.net Tue Aug 28 12:59:01 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 28 12:59:48 2007 Subject: [Histonet] Plastic microwave accessories & Fischer products In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6E5@IS-E2K3.grhs.net> I think this is advertising, but I am not an advertiser. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Tuesday, August 28, 2007 12:45 PM To: histonet@lists.utsouthwestern.edu; coleman_manufacturing@yahoo.com Subject: Re: [Histonet] Plastic microwave accessories & Fischer products Is this not blatant advertising? Or did someone inquire? Just curious. Robyn >>> "Coleman Manufacturing" 8/28/2007 7:24:53 AM >>> To all: We can supply all plastic and metal microwave accessories for any make of unit at a lower cost to you. Also, we are a certified distributor for Fischer Scientific offering all products at a lower cost to you. Please respond to this email, call, or fax. Regards, Justin Coleman Coleman Manufacturing & Design Winnsboro, SC 29180 Phone 803.633.2124 fax 803.635.9401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley_PHUA <@t> hsa.gov.sg Tue Aug 28 13:01:48 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue Aug 28 13:06:47 2007 Subject: [Histonet] Shirley Phua is away on 28 Aug 2007 (Tuesday) afternoon. Message-ID: I will be out of the office from 28-08-2007 to 29-08-2007. I'll be away on 28 Aug 2007 (Tuesday), attending a workshop in NUH. I'll be back on 29 August 2007 (Wednesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From Heidi.Miers <@t> nau.edu Tue Aug 28 13:21:53 2007 From: Heidi.Miers <@t> nau.edu (Heidi Miers) Date: Tue Aug 28 13:23:08 2007 Subject: [Histonet] Mouse Brain IHC Message-ID: <46D467C1.2020303@nau.edu> We will be doing some mouse brain IHC, looking for members of the TGF-B superfamily protein and receptor expression, specifically AMH. Has anyone done any superfamily IHC in the mouse brain and could you share your protocols for techniques? I am not sure what is the best way to perform IHC-on fresh frozen or paraffin-embedded sections. Thanks to one and all for your help! From mtarango <@t> nvcancer.org Tue Aug 28 13:28:47 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Aug 28 13:30:10 2007 Subject: [Histonet] staining mast cells and eosinophils In-Reply-To: <4a722ef70708280234y368d84d6j6930b00e8cebc5ba@mail.gmail.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6E18@NVCIEXCH02.NVCI.org> Try doing the IHC for eosinophils first and do a toluene blue for mast cells as a counter stain. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moran Elishmereni Sent: Tuesday, August 28, 2007 2:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining mast cells and eosinophils Hello, I am very new at histology and immunohistochemistry, so please excuse the simplicity of my questions. I wish to stain parrafin-embedded slides (murine/human skin and lung) for mast cells and eosinophils. That is- I want to be able to detect both cells on one slide. Is there anyway to stain with toluidine blue and also counterstain with another dye for eosinophils? Alternatively- can i stain for toluidine blue to get mast cells, and then do (on the same slide) immunohistochemistry using an anti-MBP antibody to detect eosinophils? I guess its more of a general question- can one use a simple dye AND immunohistochemistry (with antibodies) on the same slide, or is there interference? I would be very glad to receive advice and protocols. Many thanks, Moran Elishmereni Department of Pharmacology School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From carl.hobbs <@t> kcl.ac.uk Tue Aug 28 13:48:49 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue Aug 28 13:49:20 2007 Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse Message-ID: <007d01c7e9a4$12c30eb0$4101a8c0@carlba65530bda> Yep...similar probs. Got Dako's c-kit working great om pwax mouse embryos, for ICC AND Mast cells( thanks to Ole;-). In FFPW sections of adult mouse, sadly only got ICC, after HIER. Don't know why. Re NK cells...different aspect altogether, imho. Check out this site's Image gallery/Immunoforum...... http://www.immunoportal.com/index.php Carlos From HParker <@t> Skaggs.Net Tue Aug 28 15:24:41 2007 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Aug 28 15:24:59 2007 Subject: [Histonet] CPT codes In-Reply-To: <20070828171044.4180D31DB3@barracuda.skaggs.net> Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A56FE45C6@mail1-schc.skaggs.net> Hi all, Does anyone know the correct charges to charge a breast lump that must be inked. Someone told us we could only charge a -307 if it has cancer in the micro margins. As much gross work is done either way (cancer or not) so what is the real truth ? We have given most lumps 305 and I am thinking we are undercharging. Thanks, Helayne Parker, HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri From JWEEMS <@t> sjha.org Tue Aug 28 15:29:17 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Aug 28 15:29:42 2007 Subject: [Histonet] CPT codes In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A56FE45C6@mail1-schc.skaggs.net> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA328@sjhaexc02.sjha.org> 307 is to check margins whether they are malignant or not - at least that is our interpretation! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, Helayne Sent: Tuesday, August 28, 2007 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT codes Hi all, Does anyone know the correct charges to charge a breast lump that must be inked. Someone told us we could only charge a -307 if it has cancer in the micro margins. As much gross work is done either way (cancer or not) so what is the real truth ? We have given most lumps 305 and I am thinking we are undercharging. Thanks, Helayne Parker, HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From langxingpan <@t> pantomics.com Tue Aug 28 15:32:03 2007 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Tue Aug 28 15:33:11 2007 Subject: [Histonet] RE: ISH Message-ID: <005a01c7e9b2$7ec1d070$021919ac@Master> Hi Naira, Many years ago, I happened to leave a few unfixed frozen sections at room temperature over a weekend. I then fixed and hybridized these sections together with some freshly cut/fixed frozen sections with a riboprobe to Ig kappa light chain mRNA. I did not see much difference in signals among the sections, but noted that the morphology of the dried sections was much better than that of the fresh ones. Since then we dry routinely our frozen sections and cell cytospins for at least one hour before fixation for IHC and ISH. Langxing Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco CA 94107, U.S.A. Direct line: 1-510-207-9788 Fax: 1-510-653-1227 www.pantomics.com From jnocito <@t> satx.rr.com Tue Aug 28 15:51:57 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 28 15:51:24 2007 Subject: [Histonet] CPT codes References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA328@sjhaexc02.sjha.org> Message-ID: <001601c7e9b5$468c7130$d49eae18@yourxhtr8hvc4p> I agree with Joyce. If the margins were looked at, with malignant or benign, we charged a 307. JTT ----- Original Message ----- From: "Weems, Joyce" To: "Parker, Helayne" ; Sent: Tuesday, August 28, 2007 3:29 PM Subject: RE: [Histonet] CPT codes > 307 is to check margins whether they are malignant or not - at least that > is our interpretation! > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From elockman <@t> apsemail.com Tue Aug 28 16:13:06 2007 From: elockman <@t> apsemail.com (Emily Lockman) Date: Tue Aug 28 16:13:19 2007 Subject: [Histonet] Paraffin embedding tools Message-ID: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com From Jason.Wiese <@t> va.gov Tue Aug 28 16:28:15 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Aug 28 16:28:31 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12C6D@VHAV20MSGA3.v20.med.va.gov> It's called a Tamper. Tissue Tek Part number 1551 (Tamper, Large (3 per case)) Tissue Tek Part number 1552 (tamper, Small (3 per case)) There is probably a cheaper place to find them, but I don't know where. JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Lockman Sent: Tuesday, August 28, 2007 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding tools I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Tue Aug 28 16:32:30 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Aug 28 16:33:04 2007 Subject: [Histonet] Paraffin embedding tools References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AB20@LTA3VS003.ees.hhs.gov> I think they are called tampers. I use forceps but I think that's right. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Lockman Sent: Tue 8/28/2007 5:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding tools I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Tue Aug 28 17:08:43 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Aug 28 17:07:33 2007 Subject: [Histonet] RE: Histonet Digest, Vol 45, Issue 41 In-Reply-To: References: Message-ID: I do appreciate all of you who did answer on my question. I get very good suggestions. Thank you, Naira -----Original Message----- ------------------------------ Message: 6 Date: Mon, 27 Aug 2007 16:34:11 -0500 From: "Margaryan, Naira" Subject: [Histonet] ISH To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histonetters, I got a protocol for ISH for cells and there is a step, which looks very strange for me: After cytospin cells on glass slides let them air dry at RT for 60min. Is it not too long for the 60 min drying? Will cells remain on the glass in this case or there is a risk pop off cells from the glass? Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From jmahoney <@t> alegent.org Tue Aug 28 17:13:57 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Tue Aug 28 17:14:23 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> Message-ID: <46D457D50200003C0001877B@gwia.alegent.org> Emily, They are called "Tissue Tampers" and they are a Sakura product that you can get them from Cardinal. I don't think they are in the catalog but your rep should be able to find them. Jan Mahoney, Alegent health, Omaha NE >>> "Emily Lockman" 08/28/2007 4:13 PM >>> I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Tue Aug 28 17:22:17 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Aug 28 17:22:31 2007 Subject: [Histonet] APTS Coating of Microscope Slides Message-ID: Back when I was a lad I coated all of my own slides using APTS, or (3-Aminopropyl)trimethoxysilane. It worked pretty well at keeping sections on the slides. We have been using Superfrost Plus charged slides for a while now, but it is costing us about $500.00 per month just in slides. Can anyone think of a reason I ought not to go back to just coating my own slides? Will the coated slides themselves change color over time, interfere with my stains, etc? I do mostly Movat Pentachrome stains and IHC. Thanks. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union Department of Pathology _________________________________________________________________ Recharge--play some free games. Win cool prizes too! http://club.live.com/home.aspx?icid=CLUB_wlmailtextlink From gorhamk <@t> verizon.net Tue Aug 28 17:30:02 2007 From: gorhamk <@t> verizon.net (Kathy Gorham) Date: Tue Aug 28 17:30:07 2007 Subject: [Histonet] Job Posting Message-ID: <002301c7e9c2$fa64b660$2f01a8c0@kathy83b707eca> If anyone out there would like to move to a peaceful valley in Eastern Oregon (La Grande ) and would like to work .75 time with benefits I have a job for you. We are a small hospital that also covers two other hospitals plus dr's offices in 3 counties. We are looking for someone who is a HT or for someone that could sit for the exam at some point. If you are interested you can email me at gorhamk@verizon.net or call John Sanchez at 541 963 1867. Thank you Kathy Gorham, H.T. Grande Ronde Hospital 900 Sunset La Grande, Oregon 97850 541 963 1894 (5am to 3:30pm) From mickie25 <@t> netzero.net Tue Aug 28 20:00:19 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Aug 28 20:00:39 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0AB20@LTA3VS003.ees.hhs.gov> References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> <34BB307EFC9A65429BBB49E330675F7201B0AB20@LTA3VS003.ees.hhs.gov> Message-ID: Hi All, I have used the aluminum tampers. They are cut pieces of aluminum angle bar and such. One thing that works well is the wooden handle of a teasing needle. Pull out the needle (obvious I know) and use the other end. Keep it warm by sticking in the forceps warmer hole. It is much easier to use than the metal tampers. It just fits the old Tissue Tek embedding system. Good Luck! Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, August 28, 2007 2:33 PM To: Emily Lockman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin embedding tools I think they are called tampers. I use forceps but I think that's right. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Lockman Sent: Tue 8/28/2007 5:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding tools I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Aug 28 20:04:55 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 28 20:04:26 2007 Subject: [Histonet] APTS Coating of Microscope Slides References: Message-ID: <003901c7e9d8$a140ba50$d49eae18@yourxhtr8hvc4p> Jerry, I had to use FrostedPlus slides for everything because the doctors would order IHC on anything, including GMS, PAS and H&E slides. Although I told them it was expensive, it fell on deaf ears. Have you tried another company with better pricing? Have you added your time cost to prep the slides, coat them and dry them? Do you use many slides? I used to coat my slides with 15% Elmer's glue, Never again. I guess I would stay with the FrostedPlus slides, but, that's me. JTT ----- Original Message ----- From: "JR R" To: Sent: Tuesday, August 28, 2007 5:22 PM Subject: [Histonet] APTS Coating of Microscope Slides Back when I was a lad I coated all of my own slides using APTS, or (3-Aminopropyl)trimethoxysilane. It worked pretty well at keeping sections on the slides. We have been using Superfrost Plus charged slides for a while now, but it is costing us about $500.00 per month just in slides. Can anyone think of a reason I ought not to go back to just coating my own slides? Will the coated slides themselves change color over time, interfere with my stains, etc? I do mostly Movat Pentachrome stains and IHC. Thanks. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union Department of Pathology _________________________________________________________________ Recharge--play some free games. Win cool prizes too! http://club.live.com/home.aspx?icid=CLUB_wlmailtextlink_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Tue Aug 28 20:04:47 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Aug 28 20:05:05 2007 Subject: [Histonet] Job Posting In-Reply-To: <002301c7e9c2$fa64b660$2f01a8c0@kathy83b707eca> References: <002301c7e9c2$fa64b660$2f01a8c0@kathy83b707eca> Message-ID: Dear Histonetters, If there are any Mohs techs out there or histotechs interested in Mohs, Barbara Strippoli and I are giving a two day seminar covering all aspects of Mohs histology in early October. We will be using Leica 1510S cryostats. I will be happy to forward information to any interested persons. Thanks, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net From mohs76009 <@t> yahoo.com Tue Aug 28 20:37:50 2007 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Tue Aug 28 20:38:11 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: <46D457D50200003C0001877B@gwia.alegent.org> Message-ID: <7799.47250.qm@web63405.mail.re1.yahoo.com> They are in the catalog, I just ordered some. They only come in a quanity of 6. Janice Mahoney wrote: Emily, They are called "Tissue Tampers" and they are a Sakura product that you can get them from Cardinal. I don't think they are in the catalog but your rep should be able to find them. Jan Mahoney, Alegent health, Omaha NE >>> "Emily Lockman" 08/28/2007 4:13 PM >>> I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From jkiernan <@t> uwo.ca Wed Aug 29 00:31:52 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Aug 29 00:32:09 2007 Subject: [Histonet] IF-fading retardants Message-ID: Why do you "suspect you would kill your signal" by dehydrating, clearing and mounting a slide carrying a fluorescently labelled antibody? Alcohol coagulates proteins on-the-spot, complete with any covalently bound fluorescent tags. This was established for lectin histochemistry 30 years ago, and has been well documented for fluorescently labelled antibodies in more recent years. The intensity of fluorescence emission may be less in DPX than in buffered glycerol, but who has done comparisons? A fairly recent study strongly favours alcohol dehydration, clearing and mounting in a non-flourescent recinous medium. for permanent immunofluorescence slides. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: AGrobe2555@aol.com Date: Monday, August 27, 2007 14:31 Subject: Re:[Histonet] IF-fading retardants To: histonet@lists.utsouthwestern.edu > Carl, > I used to use "FluorSave" mounting medium from Calbiochem (Cat > # 345789). > This mounting medium "hardens" so the coverslip doesn't > move and you don't > have to seal around the edges with nail polish. It also > kept the signal from > fading. I still stored the slides horizontally at 4C > or -20C. I wouldn't > suggest try in to dehydrate through xylene and > coverslipping, as I suspect you > would kill your signal. > Albert > > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the > all-new AOL at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From moran.elish <@t> gmail.com Wed Aug 29 05:14:56 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Wed Aug 29 05:15:16 2007 Subject: [Histonet] staining mast cells and eosinophils In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE011B6E18@NVCIEXCH02.NVCI.org> References: <4a722ef70708280234y368d84d6j6930b00e8cebc5ba@mail.gmail.com> <5AEC610C1CE02945BD63A395BA763EDE011B6E18@NVCIEXCH02.NVCI.org> Message-ID: <4a722ef70708290314r69bf0ce8uccb732abd57d5f46@mail.gmail.com> Thanks. Can I stain the same section for toluidine blue AND eosin? Moran On 8/28/07, Tarango, Mark wrote: > > > Try doing the IHC for eosinophils first and do a toluene blue for mast > cells as a counter stain. > > > Mark Adam Tarango HT(ASCP) > > Histology & IHC Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > Direct Line (702) 822-5112 > > Mobile (702) 759-9229 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moran > Elishmereni > Sent: Tuesday, August 28, 2007 2:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] staining mast cells and eosinophils > > Hello, > > I am very new at histology and immunohistochemistry, so please excuse > the > simplicity of my questions. I wish to stain parrafin-embedded slides > (murine/human skin and lung) for mast cells and eosinophils. That is- I > want > to be able to detect both cells on one slide. Is there anyway to stain > with > toluidine blue and also counterstain with another dye for eosinophils? > Alternatively- can i stain for toluidine blue to get mast cells, and > then do > (on the same slide) immunohistochemistry using an anti-MBP antibody to > detect eosinophils? I guess its more of a general question- can one use > a > simple dye AND immunohistochemistry (with antibodies) on the same slide, > or > is there interference? I would be very glad to receive advice and > protocols. > > Many thanks, > Moran Elishmereni > > Department of Pharmacology > School of Pharmacy, Faculty of Medicine > The Hebrew University of Jerusalem > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "EMF " made the following annotations. > > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended > recipient(s) and may contain confidential, proprietary, > and/or privileged information protected by law. If you are > not the intended recipient, you may not use, copy, or > distribute this e-mail message or its attachments. If you > believe you have received this e-mail message in error, > please contact the sender by reply e-mail and destroy all > copies of the original message > > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Moran Elishmereni Department of Pharmacology School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From moran.elish <@t> gmail.com Wed Aug 29 05:54:43 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Wed Aug 29 05:54:57 2007 Subject: [Histonet] Luna's Toluidine Blue method for mast cells Message-ID: <4a722ef70708290354p352f8d9etb7208c4cffd994a4@mail.gmail.com> Hi, Can anyone give me the protocol for "Luna's Toluidine Blue Method for Mast Cells", the one which stains using eosin + tol blue? Thanks, Moran Elishmereni Department of Pharmacology School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem Email: moran.elish@gmail.com From gvdobbin <@t> ihis.org Wed Aug 29 06:21:26 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Aug 29 06:21:47 2007 Subject: [Histonet] APTS Coating of Microscope Slides Message-ID: Hi Jerry, You'll have to decide for yourself if all the manual labour involved is economical, but to answer your question: the silane imparts a positive charge on the slide. There is is no coating per se that will change color or otherwise deteriorate over time. The charge, however, can be lost if the slides get wet while in storage (ie before use). Hope this helps. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 >>> JR R 8/28/2007 7:22 PM >>> Back when I was a lad I coated all of my own slides using APTS, or (3-Aminopropyl)trimethoxysilane. It worked pretty well at keeping sections on the slides. We have been using Superfrost Plus charged slides for a while now, but it is costing us about $500.00 per month just in slides. Can anyone think of a reason I ought not to go back to just coating my own slides? Will the coated slides themselves change color over time, interfere with my stains, etc? I do mostly Movat Pentachrome stains and IHC. Thanks. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union Department of Pathology _________________________________________________________________ Recharge--play some free games. Win cool prizes too! http://club.live.com/home.aspx?icid=CLUB_wlmailtextlink_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From ROrr <@t> enh.org Wed Aug 29 06:24:48 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Aug 29 06:25:00 2007 Subject: [Histonet] another point on billing codes Message-ID: Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From Rcartun <@t> harthosp.org Wed Aug 29 07:49:25 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 29 07:50:24 2007 Subject: [Histonet] Re: [IHCRG] another point on billing codes In-Reply-To: References: Message-ID: <46D533150200007700007C5A@gwmail1.harthosp.org> Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From marijke.oste <@t> ua.ac.be Wed Aug 29 08:02:50 2007 From: marijke.oste <@t> ua.ac.be (MARIJKE OSTE) Date: Wed Aug 29 08:03:47 2007 Subject: [Histonet] TUNEL Message-ID: Hello, I'm working on tissues of the gut of preterm piglets fixated for 24h in 4% PFH and kept in ethanol. Does someone help me in finding a right protocol to stain apoptotic cells with the TUNEL technique. Thanks Yours sincerely, Marijke From elockman <@t> apsemail.com Wed Aug 29 08:06:10 2007 From: elockman <@t> apsemail.com (Emily Lockman) Date: Wed Aug 29 08:09:28 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> Message-ID: <037BDA8D37760D49A2A23D1C877EA8C930AD26@apsdc01.aps.dom> Thank you so much to everyone that responded to my question. I found exactly what I needed. Thanks again! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Lockman Sent: Tuesday, August 28, 2007 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding tools I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Aug 29 08:07:58 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 29 08:12:23 2007 Subject: [Histonet] another point on billing codes In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA334@sjhaexc02.sjha.org> This is the first that I have heard that the code for IHC might be based on negative or positive results. I believe the code addresses the stain not the results.... my 2 cents. j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Orr, Rebecca Sent: Wednesday, August 29, 2007 7:25 AM To: histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [Histonet] another point on billing codes Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Aug 29 08:16:06 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 29 08:17:07 2007 Subject: [Histonet] another point on billing codes In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA334@sjhaexc02.sjha.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA33A@sjhaexc02.sjha.org> Then again there is a different code for image analysis.. Do you mean 88342 or 88361? Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, August 29, 2007 9:08 AM To: Orr, Rebecca; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: RE: [Histonet] another point on billing codes This is the first that I have heard that the code for IHC might be based on negative or positive results. I believe the code addresses the stain not the results.... my 2 cents. j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Orr, Rebecca Sent: Wednesday, August 29, 2007 7:25 AM To: histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [Histonet] another point on billing codes Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jnocito <@t> satx.rr.com Wed Aug 29 08:24:51 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 29 08:26:28 2007 Subject: [Histonet] another point on billing codes References: Message-ID: <001501c7ea3f$fb4c7680$d49eae18@yourxhtr8hvc4p> OK, I'm not a pro (but I do play one on TV) but I checked billing at my old lab for 6 years. An immuno is an immuno is an immuno regardless of antibody and/or result. ER/PR, Her2 all have a diagnostic value whether + or -. They need to be charged. If you run a pan-CK, S100, Vimentin and a CD-45 (LCA) and the LCA is positive, do you still charge for the cytokeratin and S100? Of course you do. If you find yourself battling your billing dept. every time, do what I did. I gave them a copy of the report to show them what was going on. After a while, they stopped questioning me. As a matter of fact (and I know this is hard to believe for some of you) we became close. They were the ones who wanted a luncheon for me when I left. Thanks, my 4 cents and I hope I helped instead of causing more trouble. JTT ----- Original Message ----- From: "Orr, Rebecca" To: Cc: "IHCRG Resource Group (E-mail)" Sent: Wednesday, August 29, 2007 6:24 AM Subject: [Histonet] another point on billing codes Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Wed Aug 29 08:10:40 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Aug 29 08:42:18 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> <34BB307EFC9A65429BBB49E330675F7201B0AB20@LTA3VS003.ees.hhs.gov> Message-ID: <46D52A000200003C00018824@gwia.alegent.org> Another very creative idea one of our techs had was to use the top of a flat head screw. What is that phrase...necessity is the mother of invention? Jan Mahoney Alegent Health Omaha, NE >>> "Mickie Johnson" 08/28/2007 8:00 PM >>> Hi All, I have used the aluminum tampers. They are cut pieces of aluminum angle bar and such. One thing that works well is the wooden handle of a teasing needle. Pull out the needle (obvious I know) and use the other end. Keep it warm by sticking in the forceps warmer hole. It is much easier to use than the metal tampers. It just fits the old Tissue Tek embedding system. Good Luck! Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, August 28, 2007 2:33 PM To: Emily Lockman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin embedding tools I think they are called tampers. I use forceps but I think that's right. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Lockman Sent: Tue 8/28/2007 5:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding tools I am trying to rack my brain to come up with the name of the little stamper metal things that are used to help make tissue lay flat in the molds during embedding. If anyone knows the official name of these and where I can buy them, I would greatly appreciate it. Thanks! Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlotte.Kopczynski <@t> baycare.org Wed Aug 29 08:56:09 2007 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Wed Aug 29 08:56:52 2007 Subject: [Histonet] Part Time Histologist Message-ID: Morton Plant Mease Health System is looking for a part-time histotechnologist. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From tp2 <@t> medicine.wisc.edu Wed Aug 29 08:56:18 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Wed Aug 29 08:57:09 2007 Subject: [Histonet] TUNEL Message-ID: <46D534B3020000DF0000A3A9@gwmail.medicine.wisc.edu> Roche's TUNEL kit has directions for FFPE tissues. I've used the kit before and it worked pretty well. You can probably get a PDF of the product insert from their website. Tom >>> MARIJKE OSTE 08/29/07 8:02 AM >>> Hello, I'm working on tissues of the gut of preterm piglets fixated for 24h in 4% PFH and kept in ethanol. Does someone help me in finding a right protocol to stain apoptotic cells with the TUNEL technique. Thanks Yours sincerely, Marijke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Aug 29 09:03:33 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Aug 29 09:04:23 2007 Subject: [Histonet] Part Time Histologist Message-ID: Where is BayCare Laboratories? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Kopczynski, Charlotte" 08/29/07 9:56 AM >>> Morton Plant Mease Health System is looking for a part-time histotechnologist. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kitty.Maxey <@t> propathlab.com Wed Aug 29 09:05:50 2007 From: Kitty.Maxey <@t> propathlab.com (Kitty Maxey) Date: Wed Aug 29 09:05:57 2007 Subject: [Histonet] Histology Supervisor Opening - Dallas, TX Message-ID: HISTOLOGY SUPERVISOR ProPath(r), a progressive, CAP accredited, high-volume pathology practice in Dallas, TX needs an experienced team-oriented leader to supervisor all day-to-day operations of the histology department. Duties include but are not limited to recruiting, hiring, training and scheduling staff; supervising histology operation to ensure quality of slides and that department goals are met; maintaining preparedness for all accrediting/regulatory agency inspections, and general troubleshooting. Requirements include HT/HTL (ASCP) certification, a minimum of 5 years histology experience plus a minimum of 1 - 2 years supervisory experience. BS degree in Biological/Physical Science preferred. Must be available to work nights. ProPath utilizes leading technology and is a quality oriented pathology practice. Competitive salary with a sign-on bonus and relocation assistance offered. Medical, dental, company paid STD and LTD insurance, a matched 401K plan and more! For consideration send resume to: ProPath Human Resources 8267 Elmbrook, Suite 100 Dallas, TX 75247 214/237-1775 Fax: 214/237-1825 www.propathlab.com jobs@propathlab.com EOE. Kitty Maxey Human Resources Director ProPath - The Leader in Pathology Services ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From b-frederick <@t> northwestern.edu Wed Aug 29 09:23:02 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 29 09:23:28 2007 Subject: [Histonet] another point on billing codes In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA334@sjhaexc02.sjha.org> Message-ID: <000101c7ea48$1e6d6860$d00f7ca5@lurie.northwestern.edu> Everyone is correct- the IHC CPT code only addresses the stain,not the results. It's your work and the pathologist interpretation, not the resuts. The same could be said for special stains (say,like fungus) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, August 29, 2007 8:08 AM To: Orr, Rebecca; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: RE: [Histonet] another point on billing codes This is the first that I have heard that the code for IHC might be based on negative or positive results. I believe the code addresses the stain not the results.... my 2 cents. j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Orr, Rebecca Sent: Wednesday, August 29, 2007 7:25 AM To: histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [Histonet] another point on billing codes Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bill501 <@t> mindspring.com Wed Aug 29 09:31:48 2007 From: bill501 <@t> mindspring.com (Bill) Date: Wed Aug 29 09:32:12 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: <46D52A000200003C00018824@gwia.alegent.org> References: <037BDA8D37760D49A2A23D1C877EA8C930ACDF@apsdc01.aps.dom> <34BB307EFC9A65429BBB49E330675F7201B0AB20@LTA3VS003.ees.hhs.gov> <46D52A000200003C00018824@gwia.alegent.org> Message-ID: Histotechnology, a discipline which still has much art and continues to baffle the regulators :-) My Histotech was asking for a tamper and we discovered an aluminum pipe nail works well. Your local pipe and tobacco shop usually has a jar near checkout. They work very well. We have added a Tobacco Shop, Beauticians Supply and Dollar Store to our Lab supplier's list. Bill At 8:10 AM -0500 8/29/07, Janice Mahoney wrote: >Another very creative idea one of our techs had was to use the top of a >flat head screw. >What is that phrase...necessity is the mother of invention? -- ______________ Bill Blank, MD Heartland Lab From JWEEMS <@t> sjha.org Wed Aug 29 09:38:46 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 29 09:39:17 2007 Subject: [Histonet] Paraffin embedding tools In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA348@sjhaexc02.sjha.org> We're thrifty artistic scientists! :>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill Sent: Wednesday, August 29, 2007 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin embedding tools Histotechnology, a discipline which still has much art and continues to baffle the regulators :-) My Histotech was asking for a tamper and we discovered an aluminum pipe nail works well. Your local pipe and tobacco shop usually has a jar near checkout. They work very well. We have added a Tobacco Shop, Beauticians Supply and Dollar Store to our Lab supplier's list. Bill At 8:10 AM -0500 8/29/07, Janice Mahoney wrote: >Another very creative idea one of our techs had was to use the top of a >flat head screw. >What is that phrase...necessity is the mother of invention? -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pruegg <@t> ihctech.net Wed Aug 29 10:05:52 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Aug 29 10:07:35 2007 Subject: [Histonet] RE: [IHCRG] Re: another point on billing codes In-Reply-To: <46D533150200007700007C5A@gwmail1.harthosp.org> Message-ID: <003001c7ea4e$192bd480$6401a8c0@Patsy> All, I find this thread interesting and was wondering if I have everyone's permission to use this as one of the questions for the NSH IHC Forum? Patsy -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 6:49 AM To: rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [IHCRG] Re: another point on billing codes Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From JWEEMS <@t> sjha.org Wed Aug 29 10:26:45 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 29 10:27:13 2007 Subject: [Histonet] RE: [IHCRG] Re: another point on billing codes In-Reply-To: <003001c7ea4e$192bd480$6401a8c0@Patsy> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA354@sjhaexc02.sjha.org> Fine with me! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Wednesday, August 29, 2007 11:06 AM To: Rcartun@harthosp.org; rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: 'IHCRG Resource Group (E-mail)' Subject: [Histonet] RE: [IHCRG] Re: another point on billing codes All, I find this thread interesting and was wondering if I have everyone's permission to use this as one of the questions for the NSH IHC Forum? Patsy -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 6:49 AM To: rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [IHCRG] Re: another point on billing codes Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Rcartun <@t> harthosp.org Wed Aug 29 10:35:00 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 29 10:35:25 2007 Subject: [Histonet] [IHCRG] Re: another point on billing codes In-Reply-To: <003001c7ea4e$192bd480$6401a8c0@Patsy> References: <46D533150200007700007C5A@gwmail1.harthosp.org> <003001c7ea4e$192bd480$6401a8c0@Patsy> Message-ID: <46D559E40200007700007C73@gwmail1.harthosp.org> I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion ....... Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Patsy Ruegg" 08/29/07 11:05 AM >>> All, I find this thread interesting and was wondering if I have everyone's permission to use this as one of the questions for the NSH IHC Forum? Patsy -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 6:49 AM To: rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [IHCRG] Re: another point on billing codes Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From JWEEMS <@t> sjha.org Wed Aug 29 10:59:56 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Aug 29 11:00:34 2007 Subject: [Histonet] [IHCRG] Re: another point on billing codes In-Reply-To: <46D559E40200007700007C73@gwmail1.harthosp.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA358@sjhaexc02.sjha.org> Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 11:35 AM To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Cc: 'IHCRG Resource Group (E-mail)' Subject: [Histonet] [IHCRG] Re: another point on billing codes I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion ....... Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Patsy Ruegg" 08/29/07 11:05 AM >>> All, I find this thread interesting and was wondering if I have everyone's permission to use this as one of the questions for the NSH IHC Forum? Patsy -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 6:49 AM To: rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [IHCRG] Re: another point on billing codes Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jnocito <@t> satx.rr.com Wed Aug 29 11:34:19 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 29 11:34:51 2007 Subject: [Histonet] Re: [IHCRG] Re: another point on billing codes References: <003001c7ea4e$192bd480$6401a8c0@Patsy> Message-ID: <000901c7ea5a$756afad0$d49eae18@yourxhtr8hvc4p> I don't mind JTT ----- Original Message ----- From: "Patsy Ruegg" To: ; ; Cc: "'IHCRG Resource Group (E-mail)'" Sent: Wednesday, August 29, 2007 10:05 AM Subject: [IHCRG] Re: another point on billing codes > > All, > I find this thread interesting and was wondering if I have everyone's > permission to use this as one of the questions for the NSH IHC Forum? > Patsy > > -----Original Message----- > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of > Richard Cartun > Sent: Wednesday, August 29, 2007 6:49 AM > To: rorr@enh.org; histonet@lists.utsouthwestern.edu > Cc: IHCRG Resource Group (E-mail) > Subject: [IHCRG] Re: another point on billing codes > > > Interesting point. We use semiquantitative scoring for ER, PR, and HER2 > performed on primary breast CAs. Therefore, we use 88360x3 for these > markers whether they are positive or negative. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> "Orr, Rebecca" 08/29/07 7:24 AM >>> > > Helayne, > I'm interested in everyone's input on this thread. > Charging for Breast cases seems to be as unclear as the processing > guidelines. > We are now in the process of figuring out charges if the ER PR Her2 > results are negative. > > ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or > strongly positive, etc) > Assuming these markers are ordered on a breast cancer (not a benign > breast), even a negative result is quantitative and contributes to the > outcome of the therapy., isn't this right? > Please steer me in the right direction if this is an incorrect point. > > So we are being told by our billing folks that we must change the code > on the negative resulted ER PR her2 to a lesser charge. > I can understand if CPT may think doctors are charging on unnecessary > IHC tests, but they are focusing on the wrong tests. > (in my opinion). > A negative or 0 result on these particular markers should NOT be > synonymous with " > > Quite perplexing and frustrating. > > > ---Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, >> Helayne >> Sent: Tuesday, August 28, 2007 4:25 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] CPT codes >> >> >> Hi all, >> Does anyone know the correct charges to charge a breast lump that > must >> be inked. Someone told us we could only charge a -307 if it has > cancer >> in the micro margins. As much gross work is done either way (cancer > or >> not) so what is the real truth ? We have given most lumps 305 and I > am >> thinking we are undercharging. >> >> Thanks, >> Helayne Parker, HT (ASCP) >> Histology Section Head >> Skaggs Community Health Center >> Branson, Missouri > Becky Orr CLA,HT(ASCP)QIHC > Anatomic Pathology > Evanston Northwestern Healthcare > 847-570-2771 > > > > > > > > > --~--~---------~--~----~------------~-------~--~----~ > You received this message because you are subscribed to the Google Groups > "ihcrg" group. > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg-unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > -~----------~----~----~----~------~----~------~--~--- From relia1 <@t> earthlink.net Wed Aug 29 12:09:44 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 29 12:10:05 2007 Subject: [Histonet] RELIA Histology Job Alert Message-ID: Hi Histonetters, I have several new positions that I am excited to tell you about. Here is the information: I am currently working with a client in the Los Angeles area that is in need of 2 histo techs with IHC experience. I also have a client in the Santa Barbara area in need of a histology manager and I have a client in Northern Virginia in need of a histotech These are permanent full time dayshift positions. The clients offers a great environment, a great crew to work with and excellent salary, benefits and relocation assistance. Experienced Techs and New Grads are welcome to apply. My question is do you know of anyone who might be interested in these positions? If you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Rcartun <@t> harthosp.org Wed Aug 29 12:22:40 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 29 12:23:05 2007 Subject: [Histonet] [IHCRG] Re: another point on billing codes In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA358@sjhaexc02.sjha.org> References: <46D559E40200007700007C73@gwmail1.harthosp.org> <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA358@sjhaexc02.sjha.org> Message-ID: <46D573200200007700007C83@gwmail1.harthosp.org> Yes, you're correct. However, in my opinion, the increased RVU for the technical component of 88361 in not justified. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Weems, Joyce" 08/29/07 11:59 AM >>> Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 11:35 AM To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Cc: 'IHCRG Resource Group (E-mail)' Subject: [Histonet] [IHCRG] Re: another point on billing codes I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion ....... Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Patsy Ruegg" 08/29/07 11:05 AM >>> All, I find this thread interesting and was wondering if I have everyone's permission to use this as one of the questions for the NSH IHC Forum? Patsy -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 6:49 AM To: rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [IHCRG] Re: another point on billing codes Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jkiernan <@t> uwo.ca Wed Aug 29 12:51:16 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Aug 29 12:51:29 2007 Subject: [Histonet] staining mast cells and eosinophils In-Reply-To: <4a722ef70708280234y368d84d6j6930b00e8cebc5ba@mail.gmail.com> References: <4a722ef70708280234y368d84d6j6930b00e8cebc5ba@mail.gmail.com> Message-ID: Yes, it's easy to stain both cell-types. First stain with eosin at a high pH (such as 8) to obtain selective coloration of eosinophils. (Paneth cell granules will also stain if your tissue is intestine.) Wash in slightly acidified water, then stain with dilute toluidine blue at a low pH (less than 2 to be selective for mast cells, cartilage matrix and some types of mucus). The staining is metachromatic - purple to red, rather than blue, but not likely to be confused with the eosin colour. Rinse in slightly acidified water, dehydrate in 3 changes of 100% alcohol, clear in xylene and apply coverslip. Slightly acidified water = approx 0.5% acetic acid. Check with a microscope at both the washing stages to make sure the eosinophils and mast cells are adequately stained. As described above, nuclei should be unstained. If you want blue nuclei, use the toluidine blue at pH 3 to 4, being careful to avoid over-staining. John Kiernan Anatomy, UWO London, Canada. ----- ----- Original Message ----- From: Moran Elishmereni Date: Tuesday, August 28, 2007 5:36 Subject: [Histonet] staining mast cells and eosinophils To: histonet@lists.utsouthwestern.edu > Hello, > > I am very new at histology and immunohistochemistry, so please > excuse the > simplicity of my questions. I wish to stain parrafin-embedded slides > (murine/human skin and lung) for mast cells and eosinophils. > That is- I want > to be able to detect both cells on one slide. Is there anyway to > stain with > toluidine blue and also counterstain with another dye for eosinophils? > Alternatively- can i stain for toluidine blue to get mast cells, > and then do > (on the same slide) immunohistochemistry using an anti-MBP > antibody to > detect eosinophils? I guess its more of a general question- can > one use a > simple dye AND immunohistochemistry (with antibodies) on the > same slide, or > is there interference? I would be very glad to receive advice > and protocols. > > Many thanks, > Moran Elishmereni > > Department of Pharmacology > School of Pharmacy, Faculty of Medicine > The Hebrew University of Jerusalem > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From GDawson <@t> dynacaremilwaukee.com Wed Aug 29 13:16:33 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Aug 29 13:16:51 2007 Subject: [Histonet] [IHCRG] Re: another point on billing codes In-Reply-To: <46D573200200007700007C83@gwmail1.harthosp.org> Message-ID: If you use an FDA approved kit for the staining which not only costs much more than a routine 88342, but also requires more steps that may not fit into an IHC lab's routine protocol (since you cannot alter any step in the kit) calling for special handling and more labor, I can see justification in increased RVU for the technical component. I currently use FDA approved kits for HercepTest & EGFR staining and I can say, without question, that the technical cost, in terms of both labor and reagents, is more than when I did these two IHC's before bringing in the kits. My Opinion, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 12:23 PM To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu; Joyce Weems Cc: IHCRG Resource Group (E-mail) Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes Yes, you're correct. However, in my opinion, the increased RVU for the technical component of 88361 in not justified. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Weems, Joyce" 08/29/07 11:59 AM >>> Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 11:35 AM To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Cc: 'IHCRG Resource Group (E-mail)' Subject: [Histonet] [IHCRG] Re: another point on billing codes I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion ....... Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Patsy Ruegg" 08/29/07 11:05 AM >>> All, I find this thread interesting and was wondering if I have everyone's permission to use this as one of the questions for the NSH IHC Forum? Patsy -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2007 6:49 AM To: rorr@enh.org; histonet@lists.utsouthwestern.edu Cc: IHCRG Resource Group (E-mail) Subject: [IHCRG] Re: another point on billing codes Interesting point. We use semiquantitative scoring for ER, PR, and HER2 performed on primary breast CAs. Therefore, we use 88360x3 for these markers whether they are positive or negative. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> Helayne, I'm interested in everyone's input on this thread. Charging for Breast cases seems to be as unclear as the processing guidelines. We are now in the process of figuring out charges if the ER PR Her2 results are negative. ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or strongly positive, etc) Assuming these markers are ordered on a breast cancer (not a benign breast), even a negative result is quantitative and contributes to the outcome of the therapy., isn't this right? Please steer me in the right direction if this is an incorrect point. So we are being told by our billing folks that we must change the code on the negative resulted ER PR her2 to a lesser charge. I can understand if CPT may think doctors are charging on unnecessary IHC tests, but they are focusing on the wrong tests. (in my opinion). A negative or 0 result on these particular markers should NOT be synonymous with " Quite perplexing and frustrating. ---Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > Helayne > Sent: Tuesday, August 28, 2007 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CPT codes > > > Hi all, > Does anyone know the correct charges to charge a breast lump that must > be inked. Someone told us we could only charge a -307 if it has cancer > in the micro margins. As much gross work is done either way (cancer or > not) so what is the real truth ? We have given most lumps 305 and I am > thinking we are undercharging. > > Thanks, > Helayne Parker, HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Aug 29 13:25:26 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Aug 29 13:25:37 2007 Subject: [Histonet] Re: amyloid controls Message-ID: Steve Coakley asks about amyloid controls. They're notoriously difficult to get, and unstained sections don't keep well. What I want to know is - there are a number of methods for producing amyloidosis in experimental animals (casein injection in mice was a favorite) in the older literature. Is this animal amyloid suitable for use as an amyloid control? If it is, why isn't it available? Bob Richmond Samurai Pathologist Knoxville TN From RSRICHMOND <@t> aol.com Wed Aug 29 14:06:08 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Aug 29 14:06:20 2007 Subject: [Histonet] Re: CPT codes (breast) Message-ID: Here's chapter and verse from the College of American Pathologists (CAP) Web site, originally published in the June 2003 CAP TODAY. I confess I don't find it exactly clear! Q: Is an oriented needle-localization lumpectomy specimen, which turns out to be benign, coded as 88305 or 88307? A: The final diagnosis is not the sole determining factor in assigning the appropriate CPT code for breast excisions. If the clinical assessment and mammography findings determine the need to assess adequacy of the excision (with microscopic evaluation of the margins), and the material is prepared and evaluated, then it should be coded accordingly. In this scenario the appropriate code is 88307 Breast, Excision of Lesion, Requiring Microscopic Evaluation of Surgical Margins. Bob Richmond Samurai Pathologist Knoxville TN From carl.hobbs <@t> kcl.ac.uk Wed Aug 29 14:37:46 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Aug 29 14:38:22 2007 Subject: [Histonet] re: Mouse Brain IHC Message-ID: <000901c7ea74$13ee51c0$4101a8c0@carlba65530bda> I have used Abcam's TGF beta receptor 1 on pwax sections: pic here.... http://www.immunoportal.com/modules.php?set_albumName=album01&op=modload&name=Gallery&file=index&include=view_album.php Carl From dbpiontek <@t> hotmail.com Wed Aug 29 15:47:21 2007 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Wed Aug 29 15:47:40 2007 Subject: FW: [Histonet] Periodic Acid Formalin Fixation Message-ID: > > > Dear Histonetters:> I am performing Periodic Acid Formalin fixation on liver and noticing sinusoid shrinkage, as well as some edge effect? This is in comparison to a matched tissue control in 10% NBF. My recipe requires fixation at 4C for 48 hours via 1 gram of periodic acid in 100 mls 10% NBF. Anyone else using this fixation method? Any suggestions or advice is greatly appreciated,> > Denise Bland-Piontek, HTL(ASCP)CTBS(AATB)> NIBRI _________________________________________________________________ Messenger Caf? ? open for fun 24/7. Hot games, cool activities served daily. Visit now. http://cafemessenger.com?ocid=TXT_TAGLM_AugWLtagline From kmerriam2003 <@t> yahoo.com Thu Aug 30 08:08:25 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Aug 30 08:08:43 2007 Subject: [Histonet] IF-fading retardants Message-ID: <976643.40560.qm@web50311.mail.re2.yahoo.com> John, Are you saying that IF stained slides can go through the normal dehydration/clearing process and be mounted in a xylene-based mounting medium? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: John Kiernan To: AGrobe2555@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 29, 2007 1:31:52 AM Subject: Re: Re:[Histonet] IF-fading retardants Why do you "suspect you would kill your signal" by dehydrating, clearing and mounting a slide carrying a fluorescently labelled antibody? Alcohol coagulates proteins on-the-spot, complete with any covalently bound fluorescent tags. This was established for lectin histochemistry 30 years ago, and has been well documented for fluorescently labelled antibodies in more recent years. The intensity of fluorescence emission may be less in DPX than in buffered glycerol, but who has done comparisons? A fairly recent study strongly favours alcohol dehydration, clearing and mounting in a non-flourescent recinous medium. for permanent immunofluorescence slides. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: AGrobe2555@aol.com Date: Monday, August 27, 2007 14:31 Subject: Re:[Histonet] IF-fading retardants To: histonet@lists.utsouthwestern.edu > Carl, > I used to use "FluorSave" mounting medium from Calbiochem (Cat > # 345789). > This mounting medium "hardens" so the coverslip doesn't > move and you don't > have to seal around the edges with nail polish. It also > kept the signal from > fading. I still stored the slides horizontally at 4C > or -20C. I wouldn't > suggest try in to dehydrate through xylene and > coverslipping, as I suspect you > would kill your signal. > Albert > > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the > all-new AOL at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Need a vacation? Get great deals to amazing places on Yahoo! Travel. http://travel.yahoo.com/ From talulahgosh <@t> gmail.com Thu Aug 30 09:16:39 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Aug 30 09:16:54 2007 Subject: [Histonet] APTS/TESPA coating, other slide coating Message-ID: On this note, has anyone noticed that egg albumin-coated slides are better than TESPA (3-aminopropyltriethoxysilane) -coated slides for paraffin sectioning? We're using them for H&E and IHC. I assume that egg-albumin would have more background in IHC, but I haven't tried it yet. Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From mpence <@t> grhs.net Thu Aug 30 10:04:23 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 30 10:04:35 2007 Subject: [Histonet] peloris TP Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6F0@IS-E2K3.grhs.net> Peloris Users: I have searched the archives and read the past about users of the Peloris. We are in the market for a new TP and are looking at the Peloris as one of our chooses. I would like to hear from users that have had their TP for some time and the Pros-Cons of the unit. The vendors only give you references of those they want you to call. I want to hear from those they don't want you to call. I would ask that you please reply off-line to protect you from being "flamed". Mike Pence AP Supervisor Great River Medical Center mpence@grhs.net From mcauliff <@t> umdnj.edu Thu Aug 30 10:09:42 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 30 10:10:29 2007 Subject: [Histonet] APTS/TESPA coating, other slide coating In-Reply-To: References: Message-ID: <46D6DDB6.6090903@umdnj.edu> I think 'silane' slides are better for paraffin sections. The harsher the treatment, the better 'silane' is. Geoff Emily Sours wrote: > On this note, has anyone noticed that egg albumin-coated slides are better > than TESPA (3-aminopropyltriethoxysilane) > -coated slides for paraffin sectioning? We're using them for H&E and IHC. I > assume that egg-albumin would have more background in IHC, but I haven't > tried it yet. > > Emily > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Thu Aug 30 10:54:13 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 30 10:54:26 2007 Subject: [Histonet] APTS/TESPA coating, other slide coating In-Reply-To: Message-ID: <314106.50588.qm@web61224.mail.yahoo.com> Even if you dilute the albumen after filtering it (during preparation), you will have an increase in background noise. Ren? J Emily Sours wrote: On this note, has anyone noticed that egg albumin-coated slides are better than TESPA (3-aminopropyltriethoxysilane) -coated slides for paraffin sectioning? We're using them for H&E and IHC. I assume that egg-albumin would have more background in IHC, but I haven't tried it yet. Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From ian.montgomery <@t> bio.gla.ac.uk Thu Aug 30 11:12:29 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Aug 30 11:12:45 2007 Subject: FW: [Histonet] APTS/TESPA coating, other slide coating Message-ID: <003201c7eb20$907a1830$6424d182@IBLS.GLA.AC.UK> Geoff, As someone who routinely uses poly-l-lysine, I'm interested in your comment that silane is better for paraffin sections, why? I've also had a quick browse at the techniques for coating and although almost identical, the times in silane range from 10 seconds, Gibbs,L. Histonet Sept 06, 2001 to 5 minutes, Mutter 1988. Any thoughts on time? Ian. I think 'silane' slides are better for paraffin sections. The harsher the treatment, the better 'silane' is. Geoff Emily Sours wrote: > On this note, has anyone noticed that egg albumin-coated slides are better > than TESPA (3-aminopropyltriethoxysilane) > -coated slides for paraffin sectioning? We're using them for H&E and IHC. I > assume that egg-albumin would have more background in IHC, but I haven't > tried it yet. > > Emily > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From chill <@t> amh.org Thu Aug 30 11:12:59 2007 From: chill <@t> amh.org (Catherine Hill) Date: Thu Aug 30 11:13:47 2007 Subject: [Histonet] peloris TP Message-ID: Mike, If you would share the information you receive, or if responders would copy to me, Iwould appreciate it. We are considering purchasing a Peloris. Catherine Hill, CT(ASCP) (IAC) Anatomic Pathology Manager Abington Memorial Hospital 215-481-4393 chill@amh.org >>> "Mike Pence" 8/30/2007 11:04 AM >>> Peloris Users: I have searched the archives and read the past about users of the Peloris. We are in the market for a new TP and are looking at the Peloris as one of our chooses. I would like to hear from users that have had their TP for some time and the Pros-Cons of the unit. The vendors only give you references of those they want you to call. I want to hear from those they don't want you to call. I would ask that you please reply off-line to protect you from being "flamed". Mike Pence AP Supervisor Great River Medical Center mpence@grhs.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************** CONFIDENTIALITY NOTICE ********************** This e-mail contains LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION intended only for the use of the recipient named above. If you are not the intended recipient, you are hereby notified that any dissemination or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please notify the transmitting hospital by telephone or e-mail and delete the original e-mail received in error. THIS INFORMATION HAS BEEN DISCLOSED TO YOU FROM RECORDS WHOSE CONFIDENTIALITY IS PROTECTED BY STATE AND FEDERAL LAW. ANY FURTHER DISCLOSURE, COPYING, DISTRIBUTION OR ACTION TAKEN IN RELIANCE ON THE CONTENTS OF THESE DOCUMENTS WITHOUT THE PRIOR WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS IS PROHIBITED. YOU ARE REQUIRED TO DESTROY THE INFORMATION AFTER THE STATED NEED HAS BEEN FULFILLED. From turkekul <@t> gmail.com Thu Aug 30 12:19:37 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Aug 30 12:19:54 2007 Subject: [Histonet] IF mounting Message-ID: Hi All, We carry out IF experiments routinely and never dehydrate, clear and mount the slides. One we had to clear IF slides for Laser Capture Micro dissection and after clearing some of the slides lost the signal. I wanted to see if it is the alcohols or the xylene. After testing: some of the slides lost the signal after dehydration and some after xylene. It was depending not only on the primary but also on the secondary antibody. Cheers, Mesruh Turkekul mskcc.org New York, NY 10021 From wstover <@t> vet.uga.edu Thu Aug 30 12:32:10 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Thu Aug 30 12:32:30 2007 Subject: [Histonet] Georgia Society Message-ID: Is there anyone out there that knows how much the registration cost is for the yearly Georgia Society meeting? Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From turkekul <@t> gmail.com Thu Aug 30 12:39:57 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Aug 30 12:40:15 2007 Subject: [Histonet] methanol for embedding Message-ID: Dear Colleagues Have anyone tried methanol for dehydration for paraffin embedding? Cheers, Mesruh Turkekul mskcc.org New York, NY 10021 On 8/30/07, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RELIA Histology Job Alert (Pam Barker) > 2. RE: [IHCRG] Re: another point on billing codes (Richard Cartun) > 3. Re: staining mast cells and eosinophils (John Kiernan) > 4. RE: [IHCRG] Re: another point on billing codes (Dawson, Glen) > 5. Re: amyloid controls (Robert Richmond) > 6. Re: CPT codes (breast) (Robert Richmond) > 7. re: Mouse Brain IHC (Carl Hobbs) > 8. FW: [Histonet] Periodic Acid Formalin Fixation (Denise Piontek) > 9. Re: Re:[Histonet] IF-fading retardants (Kim Merriam) > 10. APTS/TESPA coating, other slide coating (Emily Sours) > 11. peloris TP (Mike Pence) > 12. Re: APTS/TESPA coating, other slide coating (Geoff McAuliffe) > 13. Re: APTS/TESPA coating, other slide coating (Rene J Buesa) > 14. FW: [Histonet] APTS/TESPA coating, other slide coating > (Ian Montgomery) > 15. Re: peloris TP (Catherine Hill) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 29 Aug 2007 13:09:44 -0400 > From: "Pam Barker" > Subject: [Histonet] RELIA Histology Job Alert > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > I have several new positions that I am excited to tell you about. Here > is the information: > I am currently working with a client in the Los Angeles area that is in > need of 2 histo techs with IHC experience. I also have a client in the > Santa Barbara area in need of a histology manager and I have a client in > Northern Virginia in need of a histotech These are permanent full time > dayshift positions. The clients offers a great environment, a great > crew to work with and excellent salary, benefits and relocation > assistance. > > Experienced Techs and New Grads are welcome to apply. > > My question is do you know of anyone who might be interested in these > positions? > > If you think you or someone you know might be interested please contact > me. > > I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam > > > > Thank You! > > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > > > ------------------------------ > > Message: 2 > Date: Wed, 29 Aug 2007 13:22:40 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes > To: ,, > , "Joyce Weems" > Cc: "IHCRG Resource Group \(E-mail\)" > Message-ID: <46D573200200007700007C83@gwmail1.harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Yes, you're correct. However, in my opinion, the increased RVU for the technical component of 88361 in not justified. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Weems, Joyce" 08/29/07 11:59 AM >>> > Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology. > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Wednesday, August 29, 2007 11:35 AM > To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu > Cc: 'IHCRG Resource Group (E-mail)' > Subject: [Histonet] [IHCRG] Re: another point on billing codes > > > I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion ....... > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Patsy Ruegg" 08/29/07 11:05 AM >>> > > All, > I find this thread interesting and was wondering if I have everyone's > permission to use this as one of the questions for the NSH IHC Forum? > Patsy > > -----Original Message----- > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of > Richard Cartun > Sent: Wednesday, August 29, 2007 6:49 AM > To: rorr@enh.org; histonet@lists.utsouthwestern.edu > Cc: IHCRG Resource Group (E-mail) > Subject: [IHCRG] Re: another point on billing codes > > > Interesting point. We use semiquantitative scoring for ER, PR, and HER2 > performed on primary breast CAs. Therefore, we use 88360x3 for these > markers whether they are positive or negative. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> > > Helayne, > I'm interested in everyone's input on this thread. > Charging for Breast cases seems to be as unclear as the processing > guidelines. > We are now in the process of figuring out charges if the ER PR Her2 > results are negative. > > ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or > strongly positive, etc) > Assuming these markers are ordered on a breast cancer (not a benign > breast), even a negative result is quantitative and contributes to the > outcome of the therapy., isn't this right? > Please steer me in the right direction if this is an incorrect point. > > So we are being told by our billing folks that we must change the code > on the negative resulted ER PR her2 to a lesser charge. > I can understand if CPT may think doctors are charging on unnecessary > IHC tests, but they are focusing on the wrong tests. > (in my opinion). > A negative or 0 result on these particular markers should NOT be > synonymous with " > > Quite perplexing and frustrating. > > > ---Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > > Helayne > > Sent: Tuesday, August 28, 2007 4:25 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] CPT codes > > > > > > Hi all, > > Does anyone know the correct charges to charge a breast lump that > must > > be inked. Someone told us we could only charge a -307 if it has > cancer > > in the micro margins. As much gross work is done either way (cancer > or > > not) so what is the real truth ? We have given most lumps 305 and I > am > > thinking we are undercharging. > > > > Thanks, > > Helayne Parker, HT (ASCP) > > Histology Section Head > > Skaggs Community Health Center > > Branson, Missouri > Becky Orr CLA,HT(ASCP)QIHC > Anatomic Pathology > Evanston Northwestern Healthcare > 847-570-2771 > > > > > > > > > --~--~---------~--~----~------------~-------~--~----~ > You received this message because you are subscribed to the Google Groups "ihcrg" group. > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com > For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en > -~----------~----~----~----~------~----~------~--~--- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. > > > > ------------------------------ > > Message: 3 > Date: Wed, 29 Aug 2007 13:51:16 -0400 > From: John Kiernan > Subject: Re: [Histonet] staining mast cells and eosinophils > To: Moran Elishmereni > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Yes, it's easy to stain both cell-types. First stain with eosin at a high pH (such as 8) to obtain selective coloration of eosinophils. (Paneth cell granules will also stain if your tissue is intestine.) Wash in slightly acidified water, then stain with dilute toluidine blue at a low pH (less than 2 to be selective for mast cells, cartilage matrix and some types of mucus). The staining is metachromatic - purple to red, rather than blue, but not likely to be confused with the eosin colour. Rinse in slightly acidified water, dehydrate in 3 changes of 100% alcohol, clear in xylene and apply coverslip. > Slightly acidified water = approx 0.5% acetic acid. Check with a microscope at both the washing stages to make sure the eosinophils and mast cells are adequately stained. As described above, nuclei should be unstained. If you want blue nuclei, use the toluidine blue at pH 3 to 4, being careful to avoid over-staining. > > John Kiernan > Anatomy, UWO > London, Canada. > ----- > ----- Original Message ----- > From: Moran Elishmereni > Date: Tuesday, August 28, 2007 5:36 > Subject: [Histonet] staining mast cells and eosinophils > To: histonet@lists.utsouthwestern.edu > > > Hello, > > > > I am very new at histology and immunohistochemistry, so please > > excuse the > > simplicity of my questions. I wish to stain parrafin-embedded slides > > (murine/human skin and lung) for mast cells and eosinophils. > > That is- I want > > to be able to detect both cells on one slide. Is there anyway to > > stain with > > toluidine blue and also counterstain with another dye for eosinophils? > > Alternatively- can i stain for toluidine blue to get mast cells, > > and then do > > (on the same slide) immunohistochemistry using an anti-MBP > > antibody to > > detect eosinophils? I guess its more of a general question- can > > one use a > > simple dye AND immunohistochemistry (with antibodies) on the > > same slide, or > > is there interference? I would be very glad to receive advice > > and protocols. > > > > Many thanks, > > Moran Elishmereni > > > > Department of Pharmacology > > School of Pharmacy, Faculty of Medicine > > The Hebrew University of Jerusalem > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Wed, 29 Aug 2007 13:16:33 -0500 > From: "Dawson, Glen" > Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes > To: > Cc: "IHCRG Resource Group \(E-mail\)" > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > If you use an FDA approved kit for the staining which not only costs much more than a routine 88342, but also requires more steps that may not fit into an IHC lab's routine protocol (since you cannot alter any step in the kit) calling for special handling and more labor, I can see justification in increased RVU for the technical component. I currently use FDA approved kits for HercepTest & EGFR staining and I can say, without question, that the technical cost, in terms of both labor and reagents, is more than when I did these two IHC's before bringing in the kits. > > My Opinion, > > Glen Dawson > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Wednesday, August 29, 2007 12:23 PM > To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu; > Joyce Weems > Cc: IHCRG Resource Group (E-mail) > Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes > > > Yes, you're correct. However, in my opinion, the increased RVU for the technical component of 88361 in not justified. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Weems, Joyce" 08/29/07 11:59 AM >>> > Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology. > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Wednesday, August 29, 2007 11:35 AM > To: rorr@enh.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu > Cc: 'IHCRG Resource Group (E-mail)' > Subject: [Histonet] [IHCRG] Re: another point on billing codes > > > I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion ....... > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Patsy Ruegg" 08/29/07 11:05 AM >>> > > All, > I find this thread interesting and was wondering if I have everyone's > permission to use this as one of the questions for the NSH IHC Forum? > Patsy > > -----Original Message----- > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of > Richard Cartun > Sent: Wednesday, August 29, 2007 6:49 AM > To: rorr@enh.org; histonet@lists.utsouthwestern.edu > Cc: IHCRG Resource Group (E-mail) > Subject: [IHCRG] Re: another point on billing codes > > > Interesting point. We use semiquantitative scoring for ER, PR, and HER2 > performed on primary breast CAs. Therefore, we use 88360x3 for these > markers whether they are positive or negative. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Orr, Rebecca" 08/29/07 7:24 AM >>> > > Helayne, > I'm interested in everyone's input on this thread. > Charging for Breast cases seems to be as unclear as the processing > guidelines. > We are now in the process of figuring out charges if the ER PR Her2 > results are negative. > > ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or > strongly positive, etc) > Assuming these markers are ordered on a breast cancer (not a benign > breast), even a negative result is quantitative and contributes to the > outcome of the therapy., isn't this right? > Please steer me in the right direction if this is an incorrect point. > > So we are being told by our billing folks that we must change the code > on the negative resulted ER PR her2 to a lesser charge. > I can understand if CPT may think doctors are charging on unnecessary > IHC tests, but they are focusing on the wrong tests. > (in my opinion). > A negative or 0 result on these particular markers should NOT be > synonymous with " > > Quite perplexing and frustrating. > > > ---Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Parker, > > Helayne > > Sent: Tuesday, August 28, 2007 4:25 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] CPT codes > > > > > > Hi all, > > Does anyone know the correct charges to charge a breast lump that > must > > be inked. Someone told us we could only charge a -307 if it has > cancer > > in the micro margins. As much gross work is done either way (cancer > or > > not) so what is the real truth ? We have given most lumps 305 and I > am > > thinking we are undercharging. > > > > Thanks, > > Helayne Parker, HT (ASCP) > > Histology Section Head > > Skaggs Community Health Center > > Branson, Missouri > Becky Orr CLA,HT(ASCP)QIHC > Anatomic Pathology > Evanston Northwestern Healthcare > 847-570-2771 > > > > > > > > > --~--~---------~--~----~------------~-------~--~----~ > You received this message because you are subscribed to the Google Groups "ihcrg" group. > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com > For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en > -~----------~----~----~----~------~----~------~--~--- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 29 Aug 2007 14:25:26 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: amyloid controls > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Steve Coakley asks about amyloid controls. They're notoriously > difficult to get, and unstained sections don't keep well. > > What I want to know is - there are a number of methods for producing > amyloidosis in experimental animals (casein injection in mice was a > favorite) in the older literature. Is this animal amyloid suitable for > use as an amyloid control? If it is, why isn't it available? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 6 > Date: Wed, 29 Aug 2007 15:06:08 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: CPT codes (breast) > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Here's chapter and verse from the College of American Pathologists > (CAP) Web site, originally published in the June 2003 CAP TODAY. I > confess I don't find it exactly clear! > > Q: Is an oriented needle-localization lumpectomy specimen, which turns > out to be benign, coded as 88305 or 88307? > > A: The final diagnosis is not the sole determining factor in assigning > the appropriate CPT code for breast excisions. If the clinical > assessment and mammography findings determine the need to assess > adequacy of the excision (with microscopic evaluation of the margins), > and the material is prepared and evaluated, then it should be coded > accordingly. In this scenario the appropriate code is 88307 Breast, > Excision of Lesion, Requiring Microscopic Evaluation of Surgical > Margins. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 7 > Date: Wed, 29 Aug 2007 20:37:46 +0100 > From: "Carl Hobbs" > Subject: [Histonet] re: Mouse Brain IHC > To: "Histonet" > Message-ID: <000901c7ea74$13ee51c0$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > I have used Abcam's TGF beta receptor 1 on pwax sections: pic here.... > http://www.immunoportal.com/modules.php?set_albumName=album01&op=modload&name=Gallery&file=index&include=view_album.php > Carl > > > > > ------------------------------ > > Message: 8 > Date: Wed, 29 Aug 2007 16:47:21 -0400 > From: Denise Piontek > Subject: FW: [Histonet] Periodic Acid Formalin Fixation > To: > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > > > > Dear Histonetters:> I am performing Periodic Acid Formalin fixation on liver and noticing sinusoid shrinkage, as well as some edge effect? This is in comparison to a matched tissue control in 10% NBF. My recipe requires fixation at 4C for 48 hours via 1 gram of periodic acid in 100 mls 10% NBF. Anyone else using this fixation method? Any suggestions or advice is greatly appreciated,> > Denise Bland-Piontek, HTL(ASCP)CTBS(AATB)> NIBRI > _________________________________________________________________ > Messenger Caf? ? open for fun 24/7. Hot games, cool activities served daily. Visit now. > http://cafemessenger.com?ocid=TXT_TAGLM_AugWLtagline > > ------------------------------ > > Message: 9 > Date: Thu, 30 Aug 2007 06:08:25 -0700 (PDT) > From: Kim Merriam > Subject: Re: Re:[Histonet] IF-fading retardants > To: John Kiernan , Histonet > > Message-ID: <976643.40560.qm@web50311.mail.re2.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > John, > > Are you saying that IF stained slides can go through the normal dehydration/clearing process and be mounted in a xylene-based mounting medium? > > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > > > ----- Original Message ---- > From: John Kiernan > To: AGrobe2555@aol.com > Cc: histonet@lists.utsouthwestern.edu > Sent: Wednesday, August 29, 2007 1:31:52 AM > Subject: Re: Re:[Histonet] IF-fading retardants > > > Why do you "suspect you would kill your signal" by dehydrating, clearing and mounting a slide carrying a fluorescently labelled antibody? > > Alcohol coagulates proteins on-the-spot, complete with any covalently bound fluorescent tags. This was established for lectin histochemistry 30 years ago, and has been well documented for fluorescently labelled antibodies in more recent years. The intensity of fluorescence emission may be less in DPX than in buffered glycerol, but who has done comparisons? > > A fairly recent study strongly favours alcohol dehydration, clearing and mounting in a non-flourescent recinous medium. for permanent immunofluorescence slides. > > John Kiernan > Anatomy, UWO > London, Canada > --- > ----- Original Message ----- > From: AGrobe2555@aol.com > Date: Monday, August 27, 2007 14:31 > Subject: Re:[Histonet] IF-fading retardants > To: histonet@lists.utsouthwestern.edu > > > Carl, > > I used to use "FluorSave" mounting medium from Calbiochem (Cat > > # 345789). > > This mounting medium "hardens" so the coverslip doesn't > > move and you don't > > have to seal around the edges with nail polish. It also > > kept the signal from > > fading. I still stored the slides horizontally at 4C > > or -20C. I wouldn't > > suggest try in to dehydrate through xylene and > > coverslipping, as I suspect you > > would kill your signal. > > Albert > > > > > > Albert C. Grobe, PhD > > International Heart Institute of Montana Foundation > > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > > > > > > > ************************************** Get a sneak peek of the > > all-new AOL at > > http://discover.aol.com/memed/aolcom30tour > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ____________________________________________________________________________________ > Need a vacation? Get great deals > to amazing places on Yahoo! Travel. > http://travel.yahoo.com/ > > ------------------------------ > > Message: 10 > Date: Thu, 30 Aug 2007 10:16:39 -0400 > From: "Emily Sours" > Subject: [Histonet] APTS/TESPA coating, other slide coating > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > On this note, has anyone noticed that egg albumin-coated slides are better > than TESPA (3-aminopropyltriethoxysilane) > -coated slides for paraffin sectioning? We're using them for H&E and IHC. I > assume that egg-albumin would have more background in IHC, but I haven't > tried it yet. > > Emily > -- > Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and > somebody actually proposed calling German measles, 'Liberty > measles?'...Remember when the hick legislators in certain states, in > obedience to William Jennings Bryan, who learned his biology from his pious > old grandma, set up shop as scientific experts and made the whole world > laugh itself sick by forbidding the teaching of evolution? > --Sinclair Lewis, It Can't Happen Here, 1935 > > > ------------------------------ > > Message: 11 > Date: Thu, 30 Aug 2007 10:04:23 -0500 > From: "Mike Pence" > Subject: [Histonet] peloris TP > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6F0@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Peloris Users: > > I have searched the archives and read the past about users of the > Peloris. We are in the market for a new TP and are looking at the > Peloris as one of our chooses. I would like to hear from users that > have had their TP for some time and the Pros-Cons of the unit. The > vendors only give you references of those they want you to call. I want > to hear from those they don't want you to call. I would ask that you > please reply off-line to protect you from being "flamed". > > Mike Pence > AP Supervisor > Great River Medical Center > mpence@grhs.net > > > > ------------------------------ > > Message: 12 > Date: Thu, 30 Aug 2007 11:09:42 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] APTS/TESPA coating, other slide coating > To: Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46D6DDB6.6090903@umdnj.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > I think 'silane' slides are better for paraffin sections. The harsher > the treatment, the better 'silane' is. > > Geoff > > Emily Sours wrote: > > On this note, has anyone noticed that egg albumin-coated slides are better > > than TESPA (3-aminopropyltriethoxysilane) > > -coated slides for paraffin sectioning? We're using them for H&E and IHC. I > > assume that egg-albumin would have more background in IHC, but I haven't > > tried it yet. > > > > Emily > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > > ------------------------------ > > Message: 13 > Date: Thu, 30 Aug 2007 08:54:13 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] APTS/TESPA coating, other slide coating > To: Emily Sours , > histonet@lists.utsouthwestern.edu > Message-ID: <314106.50588.qm@web61224.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Even if you dilute the albumen after filtering it (during preparation), you will have an increase in background noise. > Ren? J > > Emily Sours wrote: > On this note, has anyone noticed that egg albumin-coated slides are better > than TESPA (3-aminopropyltriethoxysilane) > -coated slides for paraffin sectioning? We're using them for H&E and IHC. I > assume that egg-albumin would have more background in IHC, but I haven't > tried it yet. > > Emily > -- > Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and > somebody actually proposed calling German measles, 'Liberty > measles?'...Remember when the hick legislators in certain states, in > obedience to William Jennings Bryan, who learned his biology from his pious > old grandma, set up shop as scientific experts and made the whole world > laugh itself sick by forbidding the teaching of evolution? > --Sinclair Lewis, It Can't Happen Here, 1935 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. > > ------------------------------ > > Message: 14 > Date: Thu, 30 Aug 2007 17:12:29 +0100 > From: "Ian Montgomery" > Subject: FW: [Histonet] APTS/TESPA coating, other slide coating > To: > Message-ID: <003201c7eb20$907a1830$6424d182@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Geoff, > As someone who routinely uses poly-l-lysine, I'm interested in your > comment that silane is better for paraffin sections, why? I've also had a > quick browse at the techniques for coating and although almost identical, > the times in silane range from 10 seconds, Gibbs,L. Histonet Sept 06, 2001 > to 5 minutes, Mutter 1988. Any thoughts on time? > Ian. > > > > I think 'silane' slides are better for paraffin sections. The harsher > the treatment, the better 'silane' is. > > Geoff > > Emily Sours wrote: > > On this note, has anyone noticed that egg albumin-coated slides are better > > than TESPA (3-aminopropyltriethoxysilane) > > -coated slides for paraffin sectioning? We're using them for H&E and IHC. > I > > assume that egg-albumin would have more background in IHC, but I haven't > > tried it yet. > > > > Emily > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > G12 8QQ. > > > > > ------------------------------ > > Message: 15 > Date: Thu, 30 Aug 2007 12:12:59 -0400 > From: "Catherine Hill" > Subject: Re: [Histonet] peloris TP > To: mpence@grhs.net, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: TEXT/plain; charset="US-ASCII" > > Mike, > If you would share the information you receive, or if responders would copy to me, Iwould appreciate it. We are considering purchasing a Peloris. > > Catherine Hill, CT(ASCP) (IAC) > Anatomic Pathology Manager > Abington Memorial Hospital > 215-481-4393 > chill@amh.org > > >>> "Mike Pence" 8/30/2007 11:04 AM >>> > Peloris Users: > > I have searched the archives and read the past about users of the > Peloris. We are in the market for a new TP and are looking at the > Peloris as one of our chooses. I would like to hear from users that > have had their TP for some time and the Pros-Cons of the unit. The > vendors only give you references of those they want you to call. I want > to hear from those they don't want you to call. I would ask that you > please reply off-line to protect you from being "flamed". > > Mike Pence > AP Supervisor > Great River Medical Center > mpence@grhs.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ****************** CONFIDENTIALITY NOTICE ********************** > > This e-mail contains LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION > intended only for the use of the recipient named above. If you are not > the intended recipient, you are hereby notified that any dissemination or > copying of this e-mail is strictly prohibited. If you have received this > e-mail in error, please notify the transmitting hospital by telephone or > e-mail and delete the original e-mail received in error. > > THIS INFORMATION HAS BEEN DISCLOSED TO YOU FROM RECORDS WHOSE > CONFIDENTIALITY IS PROTECTED BY STATE AND FEDERAL LAW. ANY FURTHER > DISCLOSURE, COPYING, DISTRIBUTION OR ACTION TAKEN IN RELIANCE ON THE > CONTENTS OF THESE DOCUMENTS WITHOUT THE PRIOR WRITTEN CONSENT OF THE > PERSON TO WHOM IT PERTAINS IS PROHIBITED. YOU ARE REQUIRED TO DESTROY > THE INFORMATION AFTER THE STATED NEED HAS BEEN FULFILLED. > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 45, Issue 45 > **************************************** > From mcauliff <@t> umdnj.edu Thu Aug 30 13:40:35 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 30 13:44:55 2007 Subject: FW: [Histonet] APTS/TESPA coating, other slide coating In-Reply-To: <003201c7eb20$907a1830$6424d182@IBLS.GLA.AC.UK> References: <003201c7eb20$907a1830$6424d182@IBLS.GLA.AC.UK> Message-ID: <46D70F23.50104@umdnj.edu> Hi Ian: I used poly-L-lysine for some time but I found that the slides lost their 'stickness' if not used in 2-3 weeks. Also, I occasionally had problems with detatchment of sections of mouse carotid artery on long immuno proceedures. I coat for about 45-60 seconds. I think the rxn. with the glass happens pretty quickly so 5 min seems like overkill to me. Geoff Ian Montgomery wrote: > Geoff, > As someone who routinely uses poly-l-lysine, I'm interested in your > comment that silane is better for paraffin sections, why? I've also had a > quick browse at the techniques for coating and although almost identical, > the times in silane range from 10 seconds, Gibbs,L. Histonet Sept 06, 2001 > to 5 minutes, Mutter 1988. Any thoughts on time? > Ian. > > > > I think 'silane' slides are better for paraffin sections. The harsher > the treatment, the better 'silane' is. > > Geoff > > Emily Sours wrote: > >> On this note, has anyone noticed that egg albumin-coated slides are better >> than TESPA (3-aminopropyltriethoxysilane) >> -coated slides for paraffin sectioning? We're using them for H&E and IHC. >> > I > >> assume that egg-albumin would have more background in IHC, but I haven't >> tried it yet. >> >> Emily >> >> > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From burch007 <@t> mc.duke.edu Thu Aug 30 15:24:43 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Aug 30 15:23:45 2007 Subject: [Histonet] Epitomics Rb MoAb CD20 Message-ID: Dear All Does anyone have any experience with the CD20 rabbit monoclonal antibody from Epitomics? I ordered and received catalog # 1632-1, lot # YD010904. My initial work up included diluting the ab 1:25 --> 1:200, HIER with Dako Target Retrieval low pH solution for 20 minutes in a 100 C water bath. Detection was with Dako Envision + for rabbit antibodies and DAB +. Human tonsil was used as control tissue. Results: zero, nada, zip, just blue. OK, cranial flatulence somewhere on my part so I repeated it the next day. Same thing. Glutton for punishment, I even tried it again. I called Epitomics and received a replacement... worked it up as above... same results. Hmmm, guess what? It's the same lot number. At least the product is consistent. I called the company again and received a replacement lot # YE042703... worked it up as above... you guessed it, same results. I don't think I'm doing anything wrong procedure wise. Has anyone tried a rabbit monoclonal CD20 from another company or had good results with this one? JB Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" From Jackie.O'Connor <@t> abbott.com Thu Aug 30 15:35:03 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Aug 30 15:36:03 2007 Subject: [Histonet] Epitomics Rb MoAb CD20 In-Reply-To: Message-ID: Isn't MOAB - Mother of all bombs? just a thought. James L Burchette Sent by: histonet-bounces@lists.utsouthwestern.edu 08/30/2007 03:24 PM To cc Subject [Histonet] Epitomics Rb MoAb CD20 Dear All Does anyone have any experience with the CD20 rabbit monoclonal antibody from Epitomics? I ordered and received catalog # 1632-1, lot # YD010904. My initial work up included diluting the ab 1:25 --> 1:200, HIER with Dako Target Retrieval low pH solution for 20 minutes in a 100 C water bath. Detection was with Dako Envision + for rabbit antibodies and DAB +. Human tonsil was used as control tissue. Results: zero, nada, zip, just blue. OK, cranial flatulence somewhere on my part so I repeated it the next day. Same thing. Glutton for punishment, I even tried it again. I called Epitomics and received a replacement... worked it up as above... same results. Hmmm, guess what? It's the same lot number. At least the product is consistent. I called the company again and received a replacement lot # YE042703... worked it up as above... you guessed it, same results. I don't think I'm doing anything wrong procedure wise. Has anyone tried a rabbit monoclonal CD20 from another company or had good results with this one? JB Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aneeshdhiman <@t> gmail.com Thu Aug 30 18:00:06 2007 From: aneeshdhiman <@t> gmail.com (aneesh Dhiman) Date: Thu Aug 30 18:00:23 2007 Subject: [Histonet] Cell block Message-ID: <69ac8f280708301600u3a79e651p275472128408b0be@mail.gmail.com> *Hi Histonetters* *This is my repeated request to anyone who has actually performed IHC on a cytology cell block which was originally received in 50% alcohol. Is it validated with histo control or similar tissue?* *Aneesh Dhiman* *BDF Medical services* *Bahrain* From cbass <@t> bidmc.harvard.edu Thu Aug 30 23:51:52 2007 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Thu Aug 30 23:52:02 2007 Subject: [Histonet] damage to dura Message-ID: <295C5D4C-0D6F-40E1-B0A6-12AD7BF74325@bidmc.harvard.edu> Hey guys, i have a question I thought may be good for the group. I am doing stereotax surgeries on rats, basically making lesions in the prefrontal cortex. There are two potential sham controls, one where a vehicle is injected the other where the holes are drilled but nothing is injected. So here's the problem. In the no injection sham (i.e. only holes are drilled) the animals display an alteration of the behavior we're interested in. The tissue looks good, and there is no sign of infiltration of immune cells in the PFC by cresyl violet. However, the dura does have some damage where the drill pierced the skull. We use a standard dremmel. Are there any suggestions for what could be going on? Is there an immune response we should specifically look for, or is there something else about the dura that could be affecting our system? Does anyone know of how dura damage can alter nearby brain areas? Any and all advice is appreciated. Caroline From cbass <@t> bidmc.harvard.edu Thu Aug 30 23:57:18 2007 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Thu Aug 30 23:57:30 2007 Subject: [Histonet] working with vibratome sections Message-ID: <5827C953-85AF-4B37-B1A1-BAFB96FED95F@bidmc.harvard.edu> Hey guys, I've been working with some vibratome sections of brain and could use some advice. I sectioned one brain and it came out ok. The blade has a tendency to hang on the last bit of tissue and flip it over producing and section with a slight thickening at the end. Despite this I've gotten some good sections, but I'm having some problems processing them. Is it possible to simply immerse some in fix? What is the best way for handling this tissue? I'd like to take some and dissect out the brain regions I'm interested in and use the rest for histology. Currently the sections are 300 microns. Is this too think to coverslip? I've cut anther brain today and it went horribly, the brain just got flattened, it seemed to get squished when the blade came along, I could get a cut but it wasn't even, and I couldn't get any useful sections. Any advice? Are there any online resources for how to effectively use the vibratome for brains. Thanks, Caroline From mmccoy100 <@t> gmail.com Fri Aug 31 02:36:03 2007 From: mmccoy100 <@t> gmail.com (Michelle McCoy) Date: Fri Aug 31 02:36:14 2007 Subject: [Histonet] State requirements Message-ID: <25355ef80708310036o7fef8ee6n759123ae94d64c41@mail.gmail.com> I'm a fairly new HT (ASCP) working in Florida. I believe some of my fellow workers are FL HT without having taken ASCP (received HT before required to take ASCP for state license). I was wondering if: -all states or only some require ASCP now -If all states requiring state licensing require ASCP to get the state license -If some states will allow FL HT (without ASCP) to work as HT in their state (grandfathered in) -who is eligible to do IHC testing in different states (in FL I believe you have to be FL/HTL licensed) -if the requirements to be HTL are the same in other states as Florida (FL HTL obtained after 5 years experience or the ASCP/HTL passed before the 5 years of experience (for those with higher degrees such as Bachelor's) Thanks for any information on this. Michelle From marijke.oste <@t> ua.ac.be Fri Aug 31 03:42:32 2007 From: marijke.oste <@t> ua.ac.be (MARIJKE OSTE) Date: Fri Aug 31 03:43:32 2007 Subject: [Histonet] TUNEL Message-ID: For staining the apoptotic cells I'm already using a kit of Roche diagnostics. I think the greatest problem is that my tissues has been saved for several years in ethanol 70%. Does anyone has experience in staining tissues for apoptotic cells who are kept in ethanol for several years? Thank you From PMcArdle <@t> ebsciences.com Fri Aug 31 05:55:01 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Fri Aug 31 05:55:20 2007 Subject: [Histonet] working with vibratome sections In-Reply-To: <5827C953-85AF-4B37-B1A1-BAFB96FED95F@bidmc.harvard.edu> References: <5827C953-85AF-4B37-B1A1-BAFB96FED95F@bidmc.harvard.edu> Message-ID: <46D7F385.20600@ebsciences.com> Hello Caroline: This is from a vendor (EBS is a Vibratome dealer): I'm sure I can get an answer for you, but I'd need some specifics: what model Vibratome, what type of blade and holder, are you using refrigeration, and any other details however insignificant they may seem (e.g., fresh brain?). Brain, as I'm sure we're all painfully aware, is notoriously finicky and "the devil is in the details." For example, pediatric brain, being more hydrated, often requires different handling from mature brain. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Caroline Bass wrote: > Hey guys, > > I've been working with some vibratome sections of brain and could use > some advice. I sectioned one brain and it came out ok. The blade has a > tendency to hang on the last bit of tissue and flip it over producing > and section with a slight thickening at the end. Despite this I've > gotten some good sections, but I'm having some problems processing > them. Is it possible to simply immerse some in fix? What is the best > way for handling this tissue? I'd like to take some and dissect out the > brain regions I'm interested in and use the rest for histology. > Currently the sections are 300 microns. Is this too think to coverslip? > > I've cut anther brain today and it went horribly, the brain just got > flattened, it seemed to get squished when the blade came along, I could > get a cut but it wasn't even, and I couldn't get any useful sections. > Any advice? Are there any online resources for how to effectively use > the vibratome for brains. > > Thanks, > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.weaver <@t> vla.defra.gsi.gov.uk Fri Aug 31 06:02:43 2007 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Fri Aug 31 06:03:00 2007 Subject: [Histonet] fixatives Message-ID: <7A885E8FE1C71C488D974EC601FAA69001DBA23B@vla-exchn1.cvlnt.vla.gov.uk> Hi everyone - can anyone tell me if you can use fixatives such as Bouins, Zenkers or any other non standard formalin fixatives in a microwave processer. Colin Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From GAshton <@t> picr.man.ac.uk Fri Aug 31 06:10:07 2007 From: GAshton <@t> picr.man.ac.uk (Garry Ashton) Date: Fri Aug 31 06:10:20 2007 Subject: [Histonet] cassette and slide labelers Message-ID: Hi, I'm interested in buying a slide and cassette labeler, both with barcode capability. I'd be very interested in peoples opinion and which type they are using. Many thanks. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From PMcArdle <@t> ebsciences.com Fri Aug 31 06:29:42 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Fri Aug 31 06:29:59 2007 Subject: [Histonet] fixatives In-Reply-To: <7A885E8FE1C71C488D974EC601FAA69001DBA23B@vla-exchn1.cvlnt.vla.gov.uk> References: <7A885E8FE1C71C488D974EC601FAA69001DBA23B@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <46D7FBA6.9090207@ebsciences.com> Dear Colin: I am with a lab microwave vendor; please don't dismiss this e-mail as you read the first couple of paragraphs that amount to a "disclaimer:" We at EBS, a pioneer in the development of laboratory microwaves, discourage the use of formalin in the microwave (see page 16 in our "Microwave Companion" booklet, http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf). Formalin is insidious due to its desensitizing action, and inherently hazardous in that 100ppm is immediately dangerous to life and health. One whiff of fumes from a tray of warm formalin can fell, if not kill, a person. This is why EBS actively promotes safe and effective alternatives to formalin such as glyoxal-based fixatives. That said, we also recognize the many compelling reasons many laboratories will continue to use formalin as long as possible, for example, the only FDA-approved HER2/neu test requires formalin fixation. So as a concession to these realities, we can make the following recommendations (however, this is not to be taken as endorsement of microwaving formalin in any form - ought to make our lawyers happy!): Depending upon the microwave processor in question, provided it is externally vented, many non-standard formalin mixtures can be safe and effective, given reasonable precautions, care, and common sense. Equipment contacting mixtures containing metals such as zinc (e.g., zinc formalin) should be kept clean after each run, in order to prevent arcing (sparking). Acidic or basic mixtures should immediately be cleaned from microwave surfaces. You mention Bouin's - naturally, you want to avoid crystallization of Bouins since solid picric acid is an explosive, but microwaving aqueous solutions per se should be OK (though flammable mixtures should be treated with some care). If you wanted to provide me a "laundry list" of precise formulations and protocols, I should be able to evaluate these for you. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Weaver, Colin wrote: > Hi everyone - can anyone tell me if you can use fixatives such as > Bouins, Zenkers or any other non standard formalin fixatives in a > microwave processer. > > Colin > > > Veterinary Laboratories Agency (VLA) > > This email and any attachments is intended for the named recipient > only. > If you have received it in error you have no authority to use, disclose, > store or copy any of its contents and you should destroy it and inform > the sender. > Whilst this email and associated attachments will have been checked > for known viruses whilst within VLA systems we can accept no > responsibility once it has left our systems. > Communications on VLA's computer systems may be monitored and/or > recorded to secure the effective operation of the system and for other > lawful purposes. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Aug 31 08:32:08 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Aug 31 08:36:30 2007 Subject: [Histonet] serra's fix question Message-ID: We have a protocol for fixing chick embryos in Serra's fix (6 100% ethanol: 3 37% formaldehyde: 1 glacial acetic acid), which states that after fixing for one to four hours, the tissue should be washed in 60% ethanol then worked up to xylene. What I don't understand is placing the embryo into a water-based wash when it was fixed in a non-water-based solution. At first I thought the 37% formaldehyde might have water, but if any water is added to the Serra's fix, it becomes milky. Am I missing something here? Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From tkngflght <@t> yahoo.com Fri Aug 31 08:54:55 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Aug 31 08:55:08 2007 Subject: [Histonet] State requirements In-Reply-To: <25355ef80708310036o7fef8ee6n759123ae94d64c41@mail.gmail.com> Message-ID: <213302.48941.qm@web50910.mail.re2.yahoo.com> Michelle- It is my understanding one can still become a HT-Florida licensed without ever having the HT(ASCP). There are many variations to answer your questions so this is a stab at the general overview of licensure, regulations and state regulations over histologists. To the best of my knowledge no states REQUIRE the ASCP examination but several states require licensure to work in labs in the state. The ASCP testing is completely independent of all states (licensure states or not)--but most states that require licensure honor (reciprocate) this test as the 'gold standard' and accept it in lieu of their own--or use it instead of proctoring their own. The grandfather for ASCP is no longer available and all candidates must qualify under the rules established by the ASCP. This information is available from the Board of Registry in a PDF booklet: http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/default.aspx This resource can answer your questions regarding the difference in requiremenst for HT vs. HTL in general, as well. As mentioned, there are only a handful of states that require licensure for Histotechs (five or six). Most states allow Histos to work without any licensure and each facility sets their standard of minimum employable requirements. Many of our temp techs have 20+ years of experience and are GREAT techs but are not registered and we can keep them working full-time. But this only answers part of your question. No state outside of Florida reciprocates with Florida licensure, only ASCP. So a FL license is not enough in itself to establish qualifications for another state's license, though most of the requisite components are the same--so you'd likely qualify. Complexity of testing and who can and can't do the testing is not regulated uniformly by state (this brings to mind the discussion on grossing--eek!). The governing bodies that have a say over this kind of testing are CAP if your facility is CAP accredited, and CLIA if you do any state or federally funded (medicaid) testing. (I"m not sure about JHACO--can someone else weigh in on this one?) ASCP will test and qualify a registered tech or even a non-histotech with a Bachelor's for IHC testing (a med tech or research assistant can become QIHC)--see the requirements via this link: http://www.ascp.org/Certification/CertifyingExaminations/qual_procedures/default.aspx This testing is validated by quality controls and monitored directly by a licensed medical director (physician) --so who can and can't do IHC is going to have a lot of discussion--for the majority of states this is controlled by the policy of the facility itself. I know this can be confusing--and as more states consider instituting state-level controls on healthcare (such as they do for other genres of healthcare) this will only get more confusing. I hope this helped a little bit--but the overall backbone to all of this is ASCP testing and as you are ASCP registered and working, you should have no worries. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time 281.852.9457 800.756.3309 Michelle McCoy wrote: I'm a fairly new HT (ASCP) working in Florida. I believe some of my fellow workers are FL HT without having taken ASCP (received HT before required to take ASCP for state license). I was wondering if: -all states or only some require ASCP now -If all states requiring state licensing require ASCP to get the state license -If some states will allow FL HT (without ASCP) to work as HT in their state (grandfathered in) -who is eligible to do IHC testing in different states (in FL I believe you have to be FL/HTL licensed) -if the requirements to be HTL are the same in other states as Florida (FL HTL obtained after 5 years experience or the ASCP/HTL passed before the 5 years of experience (for those with higher degrees such as Bachelor's) Thanks for any information on this. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Fri Aug 31 10:53:07 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Aug 31 10:53:36 2007 Subject: [Histonet] FW: Question about Tissue Capture slide adhesive Message-ID: A colleague at work asked me this question. I've never used this...does anybody have any experience with it? >>Have you ever used "Tissue Capture" (HP: http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=Z648175& Brand=ALDRICH)? I'd like to know if it works well and is applicable for cryo section. << Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From fong <@t> zoology.ubc.ca Fri Aug 31 12:13:36 2007 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Fri Aug 31 12:16:09 2007 Subject: [Histonet] Tracing Nerves with India Ink? Message-ID: <46D84C40.5040900@zoology.ubc.ca> Hi everyone, My supervisor told me and one of the grad student about tracing nerves with India Ink. We have had no luck finding any references where people have used this technique and was wondering if anyone has any experience with this? The reason we need this is to try and trace the vagus nerves in fixed reptiles to try to find some of the finer terminal branches of the vagus. We can see a large ganglion so we are inject dyes into the ganglion to allow it to travel down the nerve. The idea for using India Ink was so we can see the nerves go black without any further processing. We want to see if we can see where the nerves terminate before removing the section of blood vessels where the terminates are so we can dissect out the appropriate section for further processing. Our supervisor said he's seen it done with India Ink, but we will entertain any ideas. Appreciate any help you can give us. THANKS!! Angelina -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5990 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From dw18 <@t> uchicago.edu Fri Aug 31 12:22:48 2007 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Fri Aug 31 12:23:00 2007 Subject: [Histonet] fix & mount for clam embryos and sperm? Message-ID: <20070831122248.AQO49739@m4500-03.uchicago.edu> Hello Histonet We hope next week to fix and mount some fluorescently vitally stained bivalve (manila clams - (Rudi)Tapes philippinarum) cleaving eggs and sperm. For sperm alone, is it better to make a smear of live sperm and fix on the slide or pre-fix? Should we use PDL coated slides? APES? Subbed? SuperFrost Plus? What is the best fixative? Likewise, any recommendations for attaching cleaving eggs to a slide? Do shells need to be removed for pre-hatch stages? I am also concerned that aldehyde fixatives might increase auto- fluorescence of yolk etc. Any advice there? There was a recent thread about resin mounting fluorescently labeled materials. Any caveats about trying that with our material? Thanks, all! hope you enjoy the long weekend! -David --------------- David A. Wright PhD Biological Sciences Division University of Chicago From mcauliff <@t> umdnj.edu Fri Aug 31 13:12:43 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 31 13:14:06 2007 Subject: [Histonet] serra's fix question In-Reply-To: References: Message-ID: <46D85A1B.5000405@umdnj.edu> Hi Emily: Formaldehyde is a gas dissolved in water. Serra's fix is just another variation of formalin-alcohol-acetic acid, which is water based. If your mix is milky something is wrong. Buy fresh reagents. Geoff Emily Sours wrote: > We have a protocol for fixing chick embryos in Serra's fix (6 100% ethanol: > 3 37% formaldehyde: 1 glacial acetic acid), which states that after fixing > for one to four hours, the tissue should be washed in 60% ethanol then > worked up to xylene. What I don't understand is placing the embryo into a > water-based wash when it was fixed in a non-water-based solution. At first > I thought the 37% formaldehyde might have water, but if any water is added > to the Serra's fix, it becomes milky. Am I missing something here? > > Emily > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Fri Aug 31 13:15:49 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 31 13:17:37 2007 Subject: [Histonet] damage to dura In-Reply-To: <295C5D4C-0D6F-40E1-B0A6-12AD7BF74325@bidmc.harvard.edu> References: <295C5D4C-0D6F-40E1-B0A6-12AD7BF74325@bidmc.harvard.edu> Message-ID: <46D85AD5.9000907@umdnj.edu> If you are damaging the dura you are almost certainly disrupting/hitting the arachnoid and opening the sub-arachnoid space, thus allowing changes in CSF pressure. Geoff Caroline Bass wrote: > Hey guys, > > i have a question I thought may be good for the group. I am doing > stereotax surgeries on rats, basically making lesions in the > prefrontal cortex. There are two potential sham controls, one where a > vehicle is injected the other where the holes are drilled but nothing > is injected. So here's the problem. In the no injection sham (i.e. > only holes are drilled) the animals display an alteration of the > behavior we're interested in. The tissue looks good, and there is no > sign of infiltration of immune cells in the PFC by cresyl violet. > However, the dura does have some damage where the drill pierced the > skull. We use a standard dremmel. > > Are there any suggestions for what could be going on? Is there an > immune response we should specifically look for, or is there something > else about the dura that could be affecting our system? Does anyone > know of how dura damage can alter nearby brain areas? > > Any and all advice is appreciated. > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From PMonfils <@t> Lifespan.org Fri Aug 31 13:21:41 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Aug 31 13:21:53 2007 Subject: [Histonet] Tracing Nerves with India Ink? In-Reply-To: <46D84C40.5040900@zoology.ubc.ca> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CD8@LSRIEXCH1.lsmaster.lifespan.org> I have used India Ink for a number of purposes including tracing of vessels and brain ventricles. I have also used it extensively for marking specimens pre-processing. For example, if I need to keep track of which end of an isolated nerve is proximal and which is distal, I mark one end with India Ink. The advantage of the method is that the colorant is essentially carbon particles, and is therefore chemically inert, and is therefore not removed by any of the organic solvents used in tissue processing. Also, the ink doesn't penetrate into the tissue, but just marks the surface. Therefore the black color appears in the section as a thin black line on the surface, with no effect on internal structures. But, I find it hard to envision how it might be used to trace nerves, since nerves have no internal space that the ink can flow through, and as I said, the ink doesn't penetrate into the tissue because the colorant is particulate, not molecular in size. I wonder if your supervisor is confusing vessel-tracing techniques with nerve-tracing techniques? From mcauliff <@t> umdnj.edu Fri Aug 31 13:44:34 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 31 13:45:06 2007 Subject: [Histonet] Tracing Nerves with India Ink? In-Reply-To: <46D84C40.5040900@zoology.ubc.ca> References: <46D84C40.5040900@zoology.ubc.ca> Message-ID: <46D86192.4070907@umdnj.edu> Hi Angelina: There are lots of ways to trace nerves, both retrograde and anterograde. Go to the library and get a copy of "Neuroanatomical tract tracing Methods" by ........ I forget. Geoff Angelina Fong wrote: > Hi everyone, > > My supervisor told me and one of the grad student about tracing nerves > with India Ink. We have had no luck finding any references where > people have used this technique and was wondering if anyone has any > experience with this? > > The reason we need this is to try and trace the vagus nerves in fixed > reptiles to try to find some of the finer terminal branches of the > vagus. We can see a large ganglion so we are inject dyes into the > ganglion to allow it to travel down the nerve. The idea for using > India Ink was so we can see the nerves go black without any further > processing. We want to see if we can see where the nerves terminate > before removing the section of blood vessels where the terminates are > so we can dissect out the appropriate section for further processing. > > Our supervisor said he's seen it done with India Ink, but we will > entertain any ideas. > Appreciate any help you can give us. > THANKS!! > > Angelina > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From bob.nienhuis <@t> gmail.com Fri Aug 31 14:25:11 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Fri Aug 31 14:25:24 2007 Subject: [Histonet] Tracing Nerves with India Ink? In-Reply-To: <46D86192.4070907@umdnj.edu> References: <46D84C40.5040900@zoology.ubc.ca> <46D86192.4070907@umdnj.edu> Message-ID: <45109da50708311225i30c1be8bx94e5a580e734ea76@mail.gmail.com> I would try a carbocyanine dye such as DiI. These florescent dyes migrate in the lipid membrane of nervous tissue, and have been used in fixed tissue, but take months to get any distance. As Geoff suggests, "Neuroanatomical Tract-Tracing 3", Lazlo Zaborszky et. al, eds. has a chapter on using them. Molecular Probes (Invitrogen) sells the stuff and has info on their website. Bob On 8/31/07, Geoff McAuliffe wrote: > > Hi Angelina: > > There are lots of ways to trace nerves, both retrograde and > anterograde. Go to the library and get a copy of "Neuroanatomical tract > tracing Methods" by ........ I forget. > > Geoff > > > Angelina Fong wrote: > > Hi everyone, > > > > My supervisor told me and one of the grad student about tracing nerves > > with India Ink. We have had no luck finding any references where > > people have used this technique and was wondering if anyone has any > > experience with this? > > > > The reason we need this is to try and trace the vagus nerves in fixed > > reptiles to try to find some of the finer terminal branches of the > > vagus. We can see a large ganglion so we are inject dyes into the > > ganglion to allow it to travel down the nerve. The idea for using > > India Ink was so we can see the nerves go black without any further > > processing. We want to see if we can see where the nerves terminate > > before removing the section of blood vessels where the terminates are > > so we can dissect out the appropriate section for further processing. > > > > Our supervisor said he's seen it done with India Ink, but we will > > entertain any ideas. > > Appreciate any help you can give us. > > THANKS!! > > > > Angelina > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From STapper <@t> smdc.org Fri Aug 31 14:35:10 2007 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Fri Aug 31 14:35:28 2007 Subject: [Histonet] CPT tutorials Message-ID: Is anyone using any sort of CPT tutorial for staff development? I am trying to find something to teach staff the nuances of CPT coding...and don't want to write the class on it!!! We have the CPT book - I need scenarios and lessons, hopefully followed up with a test that could be used as a competency. Thanks! Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 Telephone: 218-786-5472 Fax: 218-786-2369 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From tkngflght <@t> yahoo.com Fri Aug 31 14:58:47 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Aug 31 14:58:28 2007 Subject: [Histonet] Does anyone have DIRECT experience working in Saudi Arabia? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273CD8@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <00d201c7ec09$58689c20$6701a8c0@CHERYLSLAPTOP> Hey everyone-- A friend is considering taking a job through an international travel company in Saudi Arabia. I knew some people who did this pre-911 but I'm sure it's changed since then...so I'd like to help her find more information on how do to this WELL (no, it's not for my company--there really is a friend, Lisa). If you've worked in this situation, would you contact me directly off the net? Cheryl tkngflght@yahoo.com or 281.883.7704 Cheryl Kerry, HT(ASCP) Full Staff Inc. From godsgalnow <@t> aol.com Fri Aug 31 15:46:16 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Aug 31 15:46:40 2007 Subject: [Histonet] education Message-ID: <8C9BA271C30FAD6-EB8-1DA1@mblk-d27.sysops.aol.com> Does anyone out there know of a college that offers an online BS program via distance education for histotechs that have an Associates degree?? If not, somebody needs to. Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From rosenfeldtek <@t> hotmail.com Fri Aug 31 17:17:51 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Aug 31 17:18:11 2007 Subject: [Histonet] TUNEL In-Reply-To: References: Message-ID: I expect you will get a very high background--lots of false positives. That's a problem with TUNEL and formalin fixed tissue anyway--formalin causes DNA strand breaks. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] TUNEL> > For staining the apoptotic cells I'm already using a kit of Roche> diagnostics. I think the greatest problem is that my tissues has been saved> for several years in ethanol 70%. Does anyone has experience in staining> tissues for apoptotic cells who are kept in ethanol for several years?> Thank you> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Explore the seven wonders of the world http://search.msn.com/results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE From koellingr <@t> comcast.net Fri Aug 31 18:17:03 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Aug 31 18:17:17 2007 Subject: [Histonet] TUNEL Message-ID: <083120072317.3363.46D8A16E000DAA4000000D2322058860149D09020704040A0105@comcast.net> Jerry, Could you expand on or give references to formalin causing DNA strand breaks? Double strand breaks, single strand, blunt end, overhanging? My pile of papers and having done TUNEL for years says that formalin fixation is a very good technique for TUNEL and many peer-reviewed articles in which TUNEL is used as a technique, use formalin fixation and how can that be if formalin is causing strand breaks? In fact one paper I'm looking at says that extended (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is causing breaks, you would assume that TUNEL pos signals would increase with extended formalin fixation. Even the use of the monoclonal antibody F7-26, for single stranded DNA, touts formalin fixation for their claims of discriminating apoptosis from necrosis. The question asks about extended alcohol fixation but your answer is possibly a lot of false positives because formalin causes DNA breaks. Does this imply that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True I couldn't pretreat them and handle them they way I would handle FFPE tissue. Also true that we could argue specificity and ability or not to discriminate apoptosis from necrosis for quite a while. But if formalin itself is causing the breaks in DNA, I and a lot of people are in big trouble with our science projects and experiments. Also I can't envision why 2 cells are showing TUNEL positivity while 2 of the same type of cells right next to them (and getting the same formalin fix), are absolutely clean and there is no background? Thanks for any information you can provide. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: JR R > > I expect you will get a very high background--lots of false positives. That's a > problem with TUNEL and formalin fixed tissue anyway--formalin causes DNA strand > breaks. > > > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: > marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> Subject: > [Histonet] TUNEL> > For staining the apoptotic cells I'm already using a kit of > Roche> diagnostics. I think the greatest problem is that my tissues has been > saved> for several years in ethanol 70%. Does anyone has experience in staining> > tissues for apoptotic cells who are kept in ethanol for several years?> Thank > you> > > _______________________________________________> Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Explore the seven wonders of the world > http://search.msn.com/results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE________ > _______________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet