[Histonet] c-fos

Turner, Scott SCOTT.TURNER <@t> SPCORP.COM
Thu Apr 26 13:09:05 CDT 2007


Are you sure it's "thermosol" and not "thimerosal"?  Thimerosal is a commonly used preservative and can be found on the Sigma website (CAS Number: 54-64-8).  Thermosol is a process used in commercial dyeing.

Scott Turner
Schering-Plough Biopharma
(formerly DNAX Research Institute)
Palo Alto, CA



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Delatour Benoît
Sent: Thursday, April 26, 2007 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] c-fos


I also confirm that fos IHC can be performed on frozen sections after 
fixation-cryoprotection without thermosol. If you really need a 
preservative you can use sodium diazide 
(http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA).

Benoît

histonet-request <@t> lists.utsouthwestern.edu a écrit :
> Message: 8
> Date: Thu, 26 Apr 2007 08:56:36 -0400
> From: jaina.negandhi <@t> sickkids.ca
> Subject: [Histonet] c-fos staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	
> <OFD12451E8.B37BC3B3-ON852572C9.004719B4-852572C9.004719BD <@t> sickkids.ca>
> 	
> Content-Type: text/plain; charset=US-ASCII
>
> I will be carrying out c-fos straining, and according to the protocol 
> I am following, I need to preserve my tissue in a mixture of succrose 
> and Thermosol. Thermosol is a preservative, however, I can't seem to 
> find it anywhere (i.e sigma, fluka etc.) Is there another 
> chemical/preservative that I can use instead, or even another 
> technique?
>
> Jaina
>   

-- 
Laboratoire de Neurobiologie de l'Apprentissage,
de la Mémoire & de la Communication,
NAMC, CNRS UMR 8620, Bât 446
Université Paris-Sud
91405 Orsay Cedex, FRANCE
Email  benoit.delatour <@t> u-psud.fr
Web    http://www.namc.u-psud.fr


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information 
included in this message is prohibited -- Please 
immediately and permanently delete.



More information about the Histonet mailing list