From dlcowie <@t> prodigy.net Sun Apr 1 04:44:49 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Sun Apr 1 04:44:53 2007 Subject: [Histonet] Re: Paper Bags In-Reply-To: <8C941F3DB9932BF-17E4-4A98@webmail-da05.sysops.aol.com> Message-ID: <20070401094449.13788.qmail@web81013.mail.mud.yahoo.com> hi Bob, As a manager, I have always shared with my staff the cost of our supplies. My staff care about what we spend and really try to be careful and not waste reagents, etc. I think part of it is that I will always try and buy the items/brands that my techs want to work with (certain kinds of microtome blades, etc.) even if they are more expensive. I think they appreciate this and therefore try to be conservative because they know I have a budget and must stick to it if at all possible. I'm pretty well left alone by my boss to purchase as I see fit and every year for past several years I have stayed within my budget (sometimes under budget) As I see it, as long as I do this, they leave me alone. I think the fact that the staff know what we pay for things really does help. As far as the tea bags go, we have found 2 things. 1. It is faster to use tea bags - both at the gross station and then at embedding. You don't have to search for the bx stuck down in the foam pad and its easier to find all the pieces. 2. If you put too many sponges in the processor, you get a lot more carry over from the reagents and therefore have to change your processor reagents more often. Summary - its not actually that much more expensive to use tea bags when you factor in the time saved at both grossing and embedding and the cost and time savings to not have to change your processing reagents more frequently. Another option for small specimens is to use the micro biopsy cassette from Surgipath. We really like these. You don't need tea bags or sponges. Instead of holes in the cassette, the bottom of the cassette is covered with a fine mesh. They work beautifully. Same kind of time savings at both grossing and embedding. The cost is about $200 for a case of 1000. We only use tea bags now for emb's and ecc's. Just pour the specimen container directly into the tea bag- you don't waste any of the specimen. Sorry for the long winded reply, I hope it helps tho. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, Florida 32503 850-416-7251 rsrichmond@aol.com wrote: Why would anyone want to use paper rather than the present-day nylon specimen bags? I recently looked at the Fisher Web site and was astonished to learn from the fisherhealthcare.com Web site, accessed a few days ago: biopsy bag 30 x 50 mm, Fisherbrand 15-182-116 500 bags for $228.17 in contrast, Fisher offers biopsy foam pads, 22-038221 1000 rectangular pads for $73.91 In other words, those nylon bags list at around 45 cents US each, while the little blue foam pads are around 7 cents each, or half that if you cut them in two as I usually do. Finding this out certainly made me change the way I use these two items - put small discrete biopsy specimens on blue pads (marked with a small drop of safranin solution), and reserve the bags for small curettage specimens, cell blocks, and things like that. I've used these two items in a good man pathology practices, but never knew the cost of them before, since catalogs are always locked up in the lab manager's office and not available to histotechnologists. Will some of you Good Managers enlighten me as to why it's Good Management Practice to have bench techs not know the cost of the items they work with? Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Sun Apr 1 09:30:49 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sun Apr 1 09:30:55 2007 Subject: [Histonet] Re: Paper Bags In-Reply-To: <8C941F3DB9932BF-17E4-4A98@webmail-da05.sysops.aol.com> References: <200703311401.288460ea1ec34d@rly-yg04.mx.aol.com> <8C941F3DB9932BF-17E4-4A98@webmail-da05.sysops.aol.com> Message-ID: <4f016b690704010730p1c0c9e91v1cfa22b1071ce55b@mail.gmail.com> Hi Bob, I really do think that many if not all managers share with their staff the cost of supplies, reagents, general supplies and many of the other direct costs associated with running a lab. What you discuss about the costs of these products isn't the whole picture though. We have to deal with the indirect costs of shipping, when it's chemical the cost of disposal of the end product and other bio hazard materials. Then there are the "built in costs", such as the amount your department is charged for all orders placed through the purchasing department - even if you place the order electronically, these are only a few of the indirect or over head costs I've had to encounter over the years. Many times we don't really have as much of the control we would like with our $$. Some institutions will cover these expenses under an operational budget that is separate to some extent, but this isn't always true in academic/research/government. It's sort of grey area that affects your budget more than you might expect. My rule has always been if the lab needs it and it is going to directly affect patient care I work to get it ordered and into the lab. Sometimes I feel more like a horse trader than a manager when it comes to vendors as I need the product, but I need to get it at the best price I can find. I might also add, that just because it's in their catalog doesn't always mean they still carry the item. With the Thermo/Fisher "merge" some manufactures have been dropped or limited in what will be distributed by them. As to the nylon bags and biopsy pads, yes they work but not for everything. The paper biopsy bags provided the ability to capture more of the small or friable tissue fragments. I agree with Dawn on everything she has said about the tea bags. We currently cannot use the mesh biopsy cassettes in our lab-but it's an excellent option that I'd like to look into given the current circumstances. I hope I haven't gotten myself into a flaming situation with what I have said, but healthcare management is like a floating iceberg these days - what you see above the water is a whole lot smaller than what is under the water. Vikki Baker NIH/NCI/LP, Bethesda, Maryland On 3/31/07, rsrichmond@aol.com wrote: > Why would anyone want to use paper rather than the present-day nylon > specimen bags? > > I recently looked at the Fisher Web site and was astonished to learn > from the > > fisherhealthcare.com Web site, accessed a few days ago: > biopsy bag 30 x 50 mm, Fisherbrand 15-182-116 > 500 bags for $228.17 > in contrast, Fisher offers biopsy foam pads, 22-038221 > 1000 rectangular pads for $73.91 > > In other words, those nylon bags list at around 45 cents US each, while > the little blue foam pads are around 7 cents each, or half that if you > cut them in two as I usually do. > > Finding this out certainly made me change the way I use these two items > - put small discrete biopsy specimens on blue pads (marked with a small > drop of safranin solution), and reserve the bags for small curettage > specimens, cell blocks, and things like that. > > I've used these two items in a good man pathology practices, but never > knew the cost of them before, since catalogs are always locked up in > the lab manager's office and not available to histotechnologists. Will > some of you Good Managers enlighten me as to why it's Good Management > Practice to have bench techs not know the cost of the items they work > with? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From karenadams <@t> comcast.net Sun Apr 1 10:07:07 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Sun Apr 1 10:07:12 2007 Subject: [Histonet] year end report.... Message-ID: <040120071507.21015.460FCA9B0000CAF60000521722058863609C030E0B0E020A9D0E05@comcast.net> I was aksed to compile a yearend report for our lab....as one has never been generated here before I would like input in everything that should be included....Thank you so much...Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From raestask <@t> grics.net Sun Apr 1 12:36:42 2007 From: raestask <@t> grics.net (Rae Staskiewiez) Date: Sun Apr 1 12:38:36 2007 Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting Message-ID: <001401c77484$54c3c650$0302a8c0@rae1ktsbyhej72> If you are planning on attending the ISH 2007 Spring Meeting May 17-18,2007 in Bloomington, IL, the cutoff for room reservations is April 16, 2007. To make your reservation, call The Chateau at 309-662-2020. Room rates are $89 + 12% tax per night. Mention that your are with the ISH when making your reservations. To view the program go to www.ilhisto.org and click on 2007 Spring Symposium. If you have questions about the program, please contact Jane Chladny at mchladny@uiuc.edu or call 217-333-8708. You may also register for the meeting at www.ilhisto.org by clicking on event registration. If you have questions about registration please contact Maureen Doran at mdoran@siumed.edu. Vendors please contact Renee Walker for information at walker2@uiuc.edu or call 217-333-8708. Rae Ann Staskiewicz Site Chair Illinois Society for Histotechnologists From tissuearray <@t> hotmail.com Sun Apr 1 13:53:50 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Sun Apr 1 13:53:55 2007 Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting In-Reply-To: <001401c77484$54c3c650$0302a8c0@rae1ktsbyhej72> Message-ID: I don't know if you are having any workshops on Tissue Microarrays but I would like you to know of instructional information at website: arrayworkshop.com It is free to all including instructional videos for constructing tissue micro arrays. Best wishes, Thom Jensen >From: "Rae Staskiewiez" >To: "Histonet" >CC: Renee Walker >Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting >Date: Sun, 1 Apr 2007 12:36:42 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc4-f23.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); Sun, 1 >Apr 2007 10:39:00 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1HY40j-0002dq-C4; Sun, 01 Apr >2007 12:38:37 -0500 >Received: from [199.242.236.161] (helo=swlx161.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1HY40g-0002dl-Kkfor >histonet@lists.utsouthwestern.edu; Sun, 01 Apr 2007 12:38:34 -0500 >Received: from thorin.grics.net ([64.40.95.86])by swlx161.swmed.edu with >esmtp (Exim 4.62)(envelope-from ) id >1HY40f-00074w-Effor histonet@lists.utsouthwestern.edu; Sun, 01 Apr 2007 >12:38:34 -0500 >Received: from localhost (localhost [127.0.0.1])by thorin.grics.net >(Postfix) with ESMTP id A510427AFD;Sun, 1 Apr 2007 12:38:32 -0500 (CDT) >Received: from thorin.grics.net ([127.0.0.1])by localhost (thorin >[127.0.0.1]) (amavisd-new, port 10024) with ESMTPid 08355-02; Sun, 1 Apr >2007 12:38:32 -0500 (CDT) >Received: from rae1ktsbyhej72 (unknown [66.79.85.161])by thorin.grics.net >(Postfix) with ESMTP id D0B0C278D3;Sun, 1 Apr 2007 12:38:31 -0500 (CDT) >X-Message-Info: >txF49lGdW42bUpMDbLbTlxDZ/s/+6k/MozgYXQGYK4cikNdeRrYEomV4DkwXl9Z6 >X-Mailer: Microsoft Office Outlook 11 >X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 >Thread-Index: Acd0hEw03ELftaxTQJa3Q2ZXTw9dEA== >X-Virus-Scanned: by amavisd-new at grics.net >X-Scan-Signature: 74c672b1d43a10f79f2b0e40e5db1c22 >X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on >swlx161.swmed.edu >X-Spam-Level: X-Spam-Status: No, score=-0.1 required=5.0 >tests=GREYLIST_ISWHITE, HTML_MESSAGEautolearn=disabled version=3.1.8 >X-Spam-Relay-Country: US ** ** US >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 218f69a45ba25cab82cec6de17a14311 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to >false >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 01 Apr 2007 17:39:00.0996 (UTC) >FILETIME=[A2400C40:01C77484] > > If you are planning on attending the ISH 2007 Spring Meeting >May >17-18,2007 in Bloomington, IL, the cutoff for room reservations is April >16, >2007. To make your reservation, call The Chateau at 309-662-2020. Room >rates >are $89 + 12% tax per night. Mention that your are with the ISH when making >your reservations. To view the program go to www.ilhisto.org > and click on 2007 Spring Symposium. If you have >questions about the program, please contact Jane Chladny at >mchladny@uiuc.edu or call 217-333-8708. You may also register for the >meeting at www.ilhisto.org by clicking on event >registration. If you have questions about registration please contact >Maureen Doran at mdoran@siumed.edu. Vendors please contact Renee Walker for >information at walker2@uiuc.edu or call 217-333-8708. > > > >Rae Ann Staskiewicz > >Site Chair > >Illinois Society for Histotechnologists > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The average US Credit Score is 675. The cost to see yours: $0 by Experian. http://www.freecreditreport.com/pm/default.aspx?sc=660600&bcd=EMAILFOOTERAVERAGE From koellingr <@t> comcast.net Sun Apr 1 19:16:38 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Apr 1 19:16:43 2007 Subject: [Histonet] Re: Paper Bags Message-ID: <040220070016.21584.46104B66000A8E9D0000545022058844849D09020704040A0105@comcast.net> To work with these kinds of specimens, for years and years in hospital used Goody curler paper (sorry I don't know anything about hairdressers curling hair but they can be had at drugstore/department store where they sell hair products). Get 500 or so for a couple of dollars. Wet them in the fixative. Mentally divide the paper into thirds each way so you have a mental image of 9 square areas in a 3x3 array. Carefully put your fragments or shavings or small biopsies in center area. Using forceps, fold up the bottom third. Fold down top third. Fold in left hand and right hand thirds to end up with square that fits in cassette and won't unfold. When processed, while unfolding you know where those bits are. Either in the center area or the single area folded over top of it. It silly, its dirt cheap, its not high-tech, its not cool or sophisticated, but for us in my lab, we never would loose any bits or pieces. Maybe we were lucky with the set-up but if I put 8 minute fragments of a gastric biopsy in, I felt confident and always could locate those 8 fragments easily for embedding. Bags I'd have trouble in creases and corners and even too with mesh cassettes. Processed well and any problem I thought might have (like getting paper shreds into block for bad sectioning), just never happened. I still think those Goody curl papers are the best. I've never had them fail to give me back every single bit of irreplaceable tissue I put in. And pretty economically. Raymond Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: rsrichmond@aol.com > Why would anyone want to use paper rather than the present-day nylon > specimen bags? > > I recently looked at the Fisher Web site and was astonished to learn > from the > > fisherhealthcare.com Web site, accessed a few days ago: > biopsy bag 30 x 50 mm, Fisherbrand 15-182-116 > 500 bags for $228.17 > in contrast, Fisher offers biopsy foam pads, 22-038221 > 1000 rectangular pads for $73.91 > > In other words, those nylon bags list at around 45 cents US each, while > the little blue foam pads are around 7 cents each, or half that if you > cut them in two as I usually do. > > Finding this out certainly made me change the way I use these two items > - put small discrete biopsy specimens on blue pads (marked with a small > drop of safranin solution), and reserve the bags for small curettage > specimens, cell blocks, and things like that. > > I've used these two items in a good man pathology practices, but never > knew the cost of them before, since catalogs are always locked up in > the lab manager's office and not available to histotechnologists. Will > some of you Good Managers enlighten me as to why it's Good Management > Practice to have bench techs not know the cost of the items they work > with? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmalc <@t> unimelb.edu.au Sun Apr 1 23:40:59 2007 From: cmalc <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Sun Apr 1 23:41:34 2007 Subject: [Histonet] Double immunostaining with a rabbit and rat primary antibody Message-ID: <6.2.1.2.2.20070402142900.04427de8@mail.staff.unimelb.edu.au> Dear All, We wish to do a double immunostain for CD34 and active caspase 3 on paraformaldehyde fixed mouse tissues. The cd34 primary is rat monoclonal anti cd34 The caspase 3 primary is a rabbit polyclonal antibody. We would like to use the Dako Envision G/2 kit for double staining, however this kit is for mouse or rabbit primaries only. When we do a single stain for cd34, we use a Rabbit anti Rat IgG as a linking antibody between the primary and secondary antibody (Dako Envision plus kit for rabbit primaries) and this works well on our tissues in our experience. My question is: Can I add this linking antibody step to the method and still use the Envision G/2 kit? Does anyone have any experience with this sort of thing or any different ideas on doing double staining using these particular antibodies? All ideas and comment appreciated as always. Cathy From mikael.niku <@t> helsinki.fi Mon Apr 2 02:20:54 2007 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Mon Apr 2 02:21:16 2007 Subject: [Histonet] Prox1 / lymphatic endothelium Message-ID: <4610AED6.1070208@helsinki.fi> Hello! I'm looking for a marker for lymphatic endothelium, which should have wide species cross-reactivity, and works with paraffin-embedded, formalin-fixed sections. Chemicon/Upstate seems to have several promising products (two monos and one polyclonal) but there's little information available on these. Anyone have experience on these or other suitable antibodies? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From Marilyn.Tyler <@t> uct.ac.za Mon Apr 2 05:09:43 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Mon Apr 2 05:10:03 2007 Subject: [Histonet] Eosinophils Message-ID: <4610F287.A78E.0090.0@uct.ac.za> Hi Again Please could you tell me what tissue in mouse is a good positive control for eosinophil staining. Thanks Marilyn From jqb7 <@t> cdc.gov Mon Apr 2 05:11:31 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Apr 2 05:11:40 2007 Subject: [Histonet] Peloris processor References: <655396.94922.qm@web61311.mail.yahoo.com> Message-ID: Please share with the whole group. Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, March 31, 2007 6:21 PM To: histonet Subject: [Histonet] Peloris processor Does anyone use the Peloris double chamber rapid tissue processor? What's it like? --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Mon Apr 2 07:57:05 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Apr 2 07:57:33 2007 Subject: [Histonet] Glycoprotein 2B, 3A Message-ID: Good morning, We are looking for a lab to do this test. thank you in advance for your help Rena Fail From rjbuesa <@t> yahoo.com Mon Apr 2 08:07:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 2 08:07:31 2007 Subject: [Histonet] Safety survey (2) Message-ID: <953403.9514.qm@web61224.mail.yahoo.com> Dear Colleagues: Since 27 March when I posted about the "safety survey" 81 colleagues asked and received the questionnaire. So far 49 have been returned which, by all standards, is a very good response,but there are 32 still missing. To those of you who received the questionnaire and have not answered it yet, could you please fill it and send it back? The more answers I get the more representative the results will be. Thank you! Ren? J. --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From lramos4 <@t> gmail.com Mon Apr 2 08:11:07 2007 From: lramos4 <@t> gmail.com (Leila Ramos) Date: Mon Apr 2 08:11:14 2007 Subject: [Histonet] Help in brain tisuue processing Message-ID: <8978a9cf0704020611qd9dc075pf85ab472e090bfea@mail.gmail.com> Dear Mr. or Ms We are working in a small lab from Canarias Island (Spain). We work with goat brain with manual procedure, our problems are several in the processing: 1. We obtain very dry blocks, when we try to cut with the microtome, the tissues are fragile and it is impossible to obtain the correct tissue. 2. When "we get proper block" (we can cut better the tissue), then we try to put in the float, the tissue appears like a white colour in the slide. After when we finish the stain process (hematoxyline-eosina and kluver barrera both of them) the tissues are not attached on the glass slides. I explain you in fast way our protocol: 1. Fixation: the whole brain is embedded in Formol 10% (1 week minimum) 2. Dehydration: 80? etanol 30 minutes; 96? 30 min x 2 times; 100 ? 30 min x 5 times; xylene 1 hour x 2 times 3. Tissue embedding in paraffin. In cassetes with pure paraffin all night- Only 1 step other times 2 changes in paraffin 1 hour 4. Cut with microtome at 8 um, slide with polylisine. 5. Stain process (Hematoxyline-Eosine and kluver barrera) Thank a lot!! Leila Ramos Instituto de Investigaci?n y Ciencias de Puerto del Rosario From JCollins <@t> palmbeachpath.com Mon Apr 2 08:53:22 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Mon Apr 2 08:53:35 2007 Subject: [Histonet] Peloris Processor Message-ID: We have the Peloris rapid tissue processor. It is xylene free but does not use microwaves. It uses isopropyl alcohol after the reagent alcohol to remove all traces of water and then goes straight to the paraffin from there. Amazingly, and contrary to everything we have been taught, this works! It processes small biopsies in approximately 1 hour. Shave biopsies take approximately 2 hours and the larger specimens 4 to 6 hours. We find the processing to be at least equal to routine processing and in many cases, superior to it. Because it does not use xylene, the tissues are not hard and brittle as they can sometimes be with routine processing. It has two separate retorts which can run two separate runs at the same time. We are able to process our specimens throughout the day instead of everything being processed at night as in the past. It has greatly improved our TAT. The Peloris tracks the number of blocks run and, based on this, tells you when to change the various solutions. As a result, we have saved a significant amount of money on reagents. We are extremely happy with ours. From mcauliff <@t> umdnj.edu Mon Apr 2 09:36:07 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Apr 2 09:35:33 2007 Subject: [Histonet] Help in brain tisuue processing In-Reply-To: <8978a9cf0704020611qd9dc075pf85ab472e090bfea@mail.gmail.com> References: <8978a9cf0704020611qd9dc075pf85ab472e090bfea@mail.gmail.com> Message-ID: <461114D7.5060402@umdnj.edu> Greetings Leila If you are fixing the whole brain by immersion the interior morphology won't be very good. I suggest cutting the brain into 1 cm slices and fixing these for 1-2 weeks. You will be able to see enough of the anatomy to know the order of the slices. If you must process the brain whole you will need to fix by vascular perfusion to see much (any) of the interior anatomy and your processing will have to be much longer. The rest of your schedule does not allow enough time for dehydration, especially on a whole brain. The tissue does not stick to the slides because it was not properly dehydrated so the paraffin does not infiltrate. On the other hand, all night in hot paraffin is too long and produces brittle tissue. I suggest the following for 1 cm slices, not a whole goat brain. After fixation, wash in water for several hours or overnight. 50% ethanol 1-2 hours. Agitation of the tissue duringhis and subsequent steps is highly desirable. 70% ethanol 1-2 hours. 95% ethanol 1 hour. 100% ethanol 2 changes 1 hour each. 1:1 mix of 100% ethanol:xylene 1 hour. Xylene, 2 changes 1 hour each. 3 changes of melted paraffin, under vacuum if possible, 45 min each. Heat the wax only a few degrees above the melting point. Embed Leila Ramos wrote: > Dear Mr. or Ms > We are working in a small lab from Canarias Island (Spain). We work with > goat brain with manual procedure, our problems are several in the > processing: > > 1. We obtain very dry blocks, when we try to cut with the microtome, > the tissues are fragile and it is impossible to obtain the correct > tissue. > 2. When "we get proper block" (we can cut better the tissue), then we > try to put in the float, the tissue appears like a white colour in the > slide. After when we finish the stain process (hematoxyline-eosina and > kluver barrera both of them) the tissues are not attached on the glass > slides. > > I explain you in fast way our protocol: > > 1. Fixation: the whole brain is embedded in Formol 10% (1 week > minimum) > 2. Dehydration: 80? etanol 30 minutes; 96? 30 min x 2 times; 100 ? 30 > min x 5 times; xylene 1 hour x 2 times > 3. Tissue embedding in paraffin. In cassetes with pure paraffin all > night- Only 1 step other times 2 changes in paraffin 1 hour > 4. Cut with microtome at 8 um, slide with polylisine. > 5. Stain process (Hematoxyline-Eosine and kluver barrera) > > Thank a lot!! > > Leila Ramos > Instituto de Investigaci?n y Ciencias de Puerto del Rosario > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Dorothy.L.Webb <@t> HealthPartners.Com Mon Apr 2 09:48:54 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Apr 2 09:49:00 2007 Subject: [Histonet] Biopsy bags Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640B56@hpes1.HealthPartners.int> We use a fair amount of the biopsy bags and one can purchase them for half the price that was quoted in the last histo-net. We purchase ours from Thermo Fisher and still get them for $205.64 for 1000 bags. We do not use the blue sponges due to the fact that we noticed reagent carryover in our processor which compromised our quality of tissue processing. Also, we have found that if one does not make certain the mesh cassettes are "submerged" in formalin a piece of tissue may stick to the top of the cassette or in a corner and not get proper processing. That is why for now we have been utilizing the nylon bags and fine them easy and efficient to use. Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Susan.Walzer <@t> HCAHealthcare.com Mon Apr 2 09:56:45 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Apr 2 09:56:53 2007 Subject: [Histonet] FW: Job Opening Message-ID: <471953BC63077941B82C26A4338272B42F050D@ORLEV03.hca.corpad.net> > -----Original Message----- > From: Walzer Susan > Sent: Wednesday, February 21, 2007 9:17 AM > To: Histonet (E-mail) > Subject: Job Opening > > We are looking for a histo-tech at St Pete. General Hospital. St Pete., FL . Apply at: > > http://www.stpetegeneralhospital.com/ > > > From ian.montgomery <@t> bio.gla.ac.uk Mon Apr 2 10:17:06 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Mon Apr 2 10:17:18 2007 Subject: Fw: [Histonet] Eosinophils Message-ID: <004701c77539$fa0a1960$4724d182@ibls.gla.ac.uk> A mouse blood smear. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Marilyn Tyler" To: "histonet" Sent: Monday, April 02, 2007 11:09 AM Subject: [Histonet] Eosinophils > Hi Again > Please could you tell me what tissue in mouse is a good positive > control for eosinophil staining. Thanks > Marilyn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Mon Apr 2 10:27:47 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Apr 2 10:26:52 2007 Subject: [Histonet] Eosinophils In-Reply-To: <4610F287.A78E.0090.0@uct.ac.za> References: <4610F287.A78E.0090.0@uct.ac.za> Message-ID: <461120F3.5030202@umdnj.edu> Small intestine. Geoff Marilyn Tyler wrote: >Hi Again >Please could you tell me what tissue in mouse is a good positive >control for eosinophil staining. Thanks >Marilyn >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From apothad <@t> gmail.com Mon Apr 2 10:38:07 2007 From: apothad <@t> gmail.com (Adam Kirk) Date: Mon Apr 2 10:38:17 2007 Subject: [Histonet] Please unsubscribe me Message-ID: <40e76ce80704020838t79db3be3tb9510420fe659a08@mail.gmail.com> Dear Histonet, Please would you unsubscribe me from your mailing list? Thanks. Adam Kirk -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 From judi.ford <@t> jax.org Mon Apr 2 10:43:33 2007 From: judi.ford <@t> jax.org (judi.ford@jax.org) Date: Mon Apr 2 10:45:17 2007 Subject: [Histonet] unsubscrible Message-ID: <12180621.1175528613818.JavaMail.ocsadmin@jcs-mid-prod.jax.org> Please unsubscribe me from the histonet list. Judi Ford HIstotechnologist From gu.lang <@t> gmx.at Mon Apr 2 11:11:57 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 2 11:12:09 2007 Subject: AW: [Histonet] Peloris Processor and isopropanol In-Reply-To: Message-ID: <001801c77541$a382c990$6412a8c0@dielangs.at> I've seen a protocol for isopropanol-processing with an IPA-temperature about 74 degrees. That is near the boilingpoint and means rather a boiling out of the tissue than acting as intermedium. Similar protocols are found with the microwave-techniques. I think there is no principal difference in reaching the temperature. What I'm a little confused about is the fact, that usually recommended temperatures for tissueprocessing don't raise above 62 degrees. So how does this influence staining, ihc-staining etc.? What are the temperatures in your protocol? What does the community think about this issue? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Judy Collins Gesendet: Montag, 02. April 2007 15:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Peloris Processor We have the Peloris rapid tissue processor. It is xylene free but does not use microwaves. It uses isopropyl alcohol after the reagent alcohol to remove all traces of water and then goes straight to the paraffin >from there. Amazingly, and contrary to everything we have been taught, this works! It processes small biopsies in approximately 1 hour. Shave biopsies take approximately 2 hours and the larger specimens 4 to 6 hours. We find the processing to be at least equal to routine processing and in many cases, superior to it. Because it does not use xylene, the tissues are not hard and brittle as they can sometimes be with routine processing. It has two separate retorts which can run two separate runs at the same time. We are able to process our specimens throughout the day instead of everything being processed at night as in the past. It has greatly improved our TAT. The Peloris tracks the number of blocks run and, based on this, tells you when to change the various solutions. As a result, we have saved a significant amount of money on reagents. We are extremely happy with ours. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mweirauch <@t> crittenton.com Mon Apr 2 11:15:14 2007 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Mon Apr 2 11:16:40 2007 Subject: [Histonet] Infectivity of FFPE tissue Message-ID: In the past, we have disposed of paraffin trimmings in regular trash and old or broken, stained and unstained slides in non-biohazard glass disposal unless they are from a suspected CJD case. We are reviewing this procedure and cannot find documentation to support our policy, in fact we have found some indication that TB may still be viable. What is everyone else doing for disposal of their paraffin debris and slides for disposal? Thanks for your input! From Charlotte.Kopczynski <@t> baycare.org Mon Apr 2 13:02:38 2007 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Mon Apr 2 13:02:49 2007 Subject: [Histonet] Florida Society for Histotechnology Meeting Notice In-Reply-To: <5pq8mh$11c6vb@intercon.baycare.org> Message-ID: FLORIDA SOCIETY FOR HISTOTECHNOLOGY ANNUAL MEETING IS APRIL 19-22 BAHIA MAR BEACH & YACHT RESORT FORT LAUDERDALE, FLORIDA Check the FSH Website @ fshgroup.org for the meeting schedule and registration information Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From Mark.Frei <@t> sial.com Mon Apr 2 13:17:11 2007 From: Mark.Frei <@t> sial.com (Mark Frei) Date: Mon Apr 2 13:17:24 2007 Subject: [Histonet] Histology Stain Contest. Submit your slides. Message-ID: Sigma-Aldrich invites histologists to participate in our histology stain contest. Gain some well-deserved attention and a chance to win valuable prizes! Stain categories include H&E and Special Stains. You have until September 28 to get your slides in the mail. Winners will be announced and prizes will be awarded at the NSH convention this October in Denver, Colorado. Visit sigma-aldrich.com/handh for more information. Good luck! Mark Frei MT(ASCP) Product Management Sigma-Aldrich Corporation 3050 Spruce Street St. Louis, MO 63103 (314) 286-8080 From pmcardle <@t> ebsciences.com Mon Apr 2 13:29:35 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Mon Apr 2 13:29:42 2007 Subject: AW: [Histonet] Peloris Processor and isopropanol In-Reply-To: <001801c77541$a382c990$6412a8c0@dielangs.at> References: <001801c77541$a382c990$6412a8c0@dielangs.at> Message-ID: <46114B8F.9070005@ebsciences.com> I'm with a laboratory microwave vendor: Never mind 74? C - some microwave protocols call for temperatures as high as 82? or 84? C (!) in order to exceed the boiling point of isopropanol, facilitating its replacement with paraffin. Naturally, this caused some concern and resistance in the anatomic pathology community. However, look at your own question about IHC: when you consider antigen retrieval protocols call for 100?C, even 84? pales by comparison. Of course, there are many reasons you want to minimize the amount of time spent at high temperatures, but there is no problem with the majority of tissue types for the relatively short timeframes involved in microwave processing. Also, by the paraffin step, the tissue has already been fixed, dehydrated, and defatted; while it is in a sense still "tissue," it's radically different from the tissue as received. You certainly don?t want to burn or "cook" anything, but 82?C isn?t really extreme in this context. (I'd be at least as concerned about how a given paraffin and additives hold up at high temperatures, so it's always a good idea to check with the manufacturer.) There also is the issue of vacuum; since the purpose of high temperature is to get above the boiling point of isopropanol, and vacuum causes BP depression, vacuum should allow you to lower the temperature to something you're more comfortable with. More information may be found at http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf Very best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Gudrun Lang wrote: > I've seen a protocol for isopropanol-processing with an IPA-temperature > about 74 degrees. That is near the boilingpoint and means rather a boiling > out of the tissue than acting as intermedium. Similar protocols are found > with the microwave-techniques. I think there is no principal difference in > reaching the temperature. > > What I'm a little confused about is the fact, that usually recommended > temperatures for tissueprocessing don't raise above 62 degrees. So how does > this influence staining, ihc-staining etc.? > What are the temperatures in your protocol? > What does the community think about this issue? > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Judy > Collins > Gesendet: Montag, 02. April 2007 15:53 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Peloris Processor > > We have the Peloris rapid tissue processor. It is xylene free but does > not use microwaves. It uses isopropyl alcohol after the reagent alcohol > to remove all traces of water and then goes straight to the paraffin > >from there. Amazingly, and contrary to everything we have been taught, > this works! > > It processes small biopsies in approximately 1 hour. Shave biopsies > take approximately 2 hours and the larger specimens 4 to 6 hours. We > find the processing to be at least equal to routine processing and in > many cases, superior to it. Because it does not use xylene, the tissues > are not hard and brittle as they can sometimes be with routine > processing. > > It has two separate retorts which can run two separate runs at the same > time. We are able to process our specimens throughout the day instead > of everything being processed at night as in the past. It has greatly > improved our TAT. > > The Peloris tracks the number of blocks run and, based on this, tells > you when to change the various solutions. As a result, we have saved a > significant amount of money on reagents. > > We are extremely happy with ours. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From linda <@t> biocare.net Mon Apr 2 14:11:50 2007 From: linda <@t> biocare.net (Linda Dean) Date: Mon Apr 2 14:12:19 2007 Subject: [Histonet] Double immunostaining with a rabbit and rat primary antibody In-Reply-To: <200704021701.l32H1o4N020917@inbound-mx5.atl.registeredsite.com> Message-ID: <20070402191150.KFSP1942.imta06a2.registeredsite.com@LDean> Dear Cathy, You may want to use an anti-rat polymer for this double stain. BIOCARE Medical makes a Rat 0n Mouse polymer in either HRP or ALP. Please contact me for further information. All the Best, Linda Dean Biocare Medical Research Sales (925) 603-8025 cell: (925)640-0074 Linda@biocare.net BIOCARE MEDICAL LLC. 4040 Pike Lane Concord, CA 94520 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, April 02, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 41, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Illinois Society for Histotechnologists Spring Meeting (Rae Staskiewiez) 2. RE: Illinois Society for Histotechnologists Spring Meeting (Thom Jensen) 3. Re: Re: Paper Bags (koellingr@comcast.net) 4. Double immunostaining with a rabbit and rat primary antibody (Cathy Malcontenti-Wilson) 5. Prox1 / lymphatic endothelium (Mikael Niku) 6. Eosinophils (Marilyn Tyler) 7. RE: Peloris processor (Bartlett, Jeanine (CDC/CCID/NCZVED)) 8. Glycoprotein 2B, 3A (Mildred Fail) 9. Safety survey (2) (Rene J Buesa) 10. Help in brain tisuue processing (Leila Ramos) 11. Peloris Processor (Judy Collins) 12. Re: Help in brain tisuue processing (Geoff McAuliffe) 13. Biopsy bags (Webb, Dorothy L) 14. FW: Job Opening (Walzer Susan) 15. Fw: [Histonet] Eosinophils (Ian Montgomery) 16. Re: Eosinophils (Geoff McAuliffe) 17. Please unsubscribe me (Adam Kirk) 18. unsubscrible (judi.ford@jax.org) 19. AW: [Histonet] Peloris Processor and isopropanol (Gudrun Lang) 20. Infectivity of FFPE tissue (Maray Weirauch) ---------------------------------------------------------------------- Message: 1 Date: Sun, 1 Apr 2007 12:36:42 -0500 From: "Rae Staskiewiez" Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting To: "Histonet" Cc: Renee Walker Message-ID: <001401c77484$54c3c650$0302a8c0@rae1ktsbyhej72> Content-Type: text/plain; charset="us-ascii" If you are planning on attending the ISH 2007 Spring Meeting May 17-18,2007 in Bloomington, IL, the cutoff for room reservations is April 16, 2007. To make your reservation, call The Chateau at 309-662-2020. Room rates are $89 + 12% tax per night. Mention that your are with the ISH when making your reservations. To view the program go to www.ilhisto.org and click on 2007 Spring Symposium. If you have questions about the program, please contact Jane Chladny at mchladny@uiuc.edu or call 217-333-8708. You may also register for the meeting at www.ilhisto.org by clicking on event registration. If you have questions about registration please contact Maureen Doran at mdoran@siumed.edu. Vendors please contact Renee Walker for information at walker2@uiuc.edu or call 217-333-8708. Rae Ann Staskiewicz Site Chair Illinois Society for Histotechnologists ------------------------------ Message: 2 Date: Sun, 01 Apr 2007 18:53:50 +0000 From: "Thom Jensen" Subject: RE: [Histonet] Illinois Society for Histotechnologists Spring Meeting To: raestask@grics.net, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed I don't know if you are having any workshops on Tissue Microarrays but I would like you to know of instructional information at website: arrayworkshop.com It is free to all including instructional videos for constructing tissue micro arrays. Best wishes, Thom Jensen >From: "Rae Staskiewiez" >To: "Histonet" >CC: Renee Walker >Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting >Date: Sun, 1 Apr 2007 12:36:42 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc4-f23.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); Sun, 1 >Apr 2007 10:39:00 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1HY40j-0002dq-C4; Sun, 01 Apr >2007 12:38:37 -0500 >Received: from [199.242.236.161] (helo=swlx161.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1HY40g-0002dl-Kkfor >histonet@lists.utsouthwestern.edu; Sun, 01 Apr 2007 12:38:34 -0500 >Received: from thorin.grics.net ([64.40.95.86])by swlx161.swmed.edu with >esmtp (Exim 4.62)(envelope-from ) id >1HY40f-00074w-Effor histonet@lists.utsouthwestern.edu; Sun, 01 Apr 2007 >12:38:34 -0500 >Received: from localhost (localhost [127.0.0.1])by thorin.grics.net >(Postfix) with ESMTP id A510427AFD;Sun, 1 Apr 2007 12:38:32 -0500 (CDT) >Received: from thorin.grics.net ([127.0.0.1])by localhost (thorin >[127.0.0.1]) (amavisd-new, port 10024) with ESMTPid 08355-02; Sun, 1 Apr >2007 12:38:32 -0500 (CDT) >Received: from rae1ktsbyhej72 (unknown [66.79.85.161])by thorin.grics.net >(Postfix) with ESMTP id D0B0C278D3;Sun, 1 Apr 2007 12:38:31 -0500 (CDT) >X-Message-Info: >txF49lGdW42bUpMDbLbTlxDZ/s/+6k/MozgYXQGYK4cikNdeRrYEomV4DkwXl9Z6 >X-Mailer: Microsoft Office Outlook 11 >X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 >Thread-Index: Acd0hEw03ELftaxTQJa3Q2ZXTw9dEA== >X-Virus-Scanned: by amavisd-new at grics.net >X-Scan-Signature: 74c672b1d43a10f79f2b0e40e5db1c22 >X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on >swlx161.swmed.edu >X-Spam-Level: X-Spam-Status: No, score=-0.1 required=5.0 >tests=GREYLIST_ISWHITE, HTML_MESSAGEautolearn=disabled version=3.1.8 >X-Spam-Relay-Country: US ** ** US >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 218f69a45ba25cab82cec6de17a14311 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to >false >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 01 Apr 2007 17:39:00.0996 (UTC) >FILETIME=[A2400C40:01C77484] > > If you are planning on attending the ISH 2007 Spring Meeting >May >17-18,2007 in Bloomington, IL, the cutoff for room reservations is April >16, >2007. To make your reservation, call The Chateau at 309-662-2020. Room >rates >are $89 + 12% tax per night. Mention that your are with the ISH when making >your reservations. To view the program go to www.ilhisto.org > and click on 2007 Spring Symposium. If you have >questions about the program, please contact Jane Chladny at >mchladny@uiuc.edu or call 217-333-8708. You may also register for the >meeting at www.ilhisto.org by clicking on event >registration. If you have questions about registration please contact >Maureen Doran at mdoran@siumed.edu. Vendors please contact Renee Walker for >information at walker2@uiuc.edu or call 217-333-8708. > > > >Rae Ann Staskiewicz > >Site Chair > >Illinois Society for Histotechnologists > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The average US Credit Score is 675. The cost to see yours: $0 by Experian. http://www.freecreditreport.com/pm/default.aspx?sc=660600&bcd=EMAILFOOTERAVE RAGE ------------------------------ Message: 3 Date: Mon, 02 Apr 2007 00:16:38 +0000 From: koellingr@comcast.net Subject: Re: [Histonet] Re: Paper Bags To: histonet@lists.utsouthwestern.edu Message-ID: <040220070016.21584.46104B66000A8E9D0000545022058844849D09020704040A0105@com cast.net> Content-Type: text/plain To work with these kinds of specimens, for years and years in hospital used Goody curler paper (sorry I don't know anything about hairdressers curling hair but they can be had at drugstore/department store where they sell hair products). Get 500 or so for a couple of dollars. Wet them in the fixative. Mentally divide the paper into thirds each way so you have a mental image of 9 square areas in a 3x3 array. Carefully put your fragments or shavings or small biopsies in center area. Using forceps, fold up the bottom third. Fold down top third. Fold in left hand and right hand thirds to end up with square that fits in cassette and won't unfold. When processed, while unfolding you know where those bits are. Either in the center area or the single area folded over top of it. It silly, its dirt cheap, its not high-tech, its not cool or sophisticated, but for us in my lab, we never would loose any bits or pieces. Maybe we were lucky with the set-up but if I put 8 minute fragments of a gastric biopsy in, I felt confident and always could locate those 8 fragments easily for embedding. Bags I'd have trouble in creases and corners and even too with mesh cassettes. Processed well and any problem I thought might have (like getting paper shreds into block for bad sectioning), just never happened. I still think those Goody curl papers are the best. I've never had them fail to give me back every single bit of irreplaceable tissue I put in. And pretty economically. Raymond Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: rsrichmond@aol.com > Why would anyone want to use paper rather than the present-day nylon > specimen bags? > > I recently looked at the Fisher Web site and was astonished to learn > from the > > fisherhealthcare.com Web site, accessed a few days ago: > biopsy bag 30 x 50 mm, Fisherbrand 15-182-116 > 500 bags for $228.17 > in contrast, Fisher offers biopsy foam pads, 22-038221 > 1000 rectangular pads for $73.91 > > In other words, those nylon bags list at around 45 cents US each, while > the little blue foam pads are around 7 cents each, or half that if you > cut them in two as I usually do. > > Finding this out certainly made me change the way I use these two items > - put small discrete biopsy specimens on blue pads (marked with a small > drop of safranin solution), and reserve the bags for small curettage > specimens, cell blocks, and things like that. > > I've used these two items in a good man pathology practices, but never > knew the cost of them before, since catalogs are always locked up in > the lab manager's office and not available to histotechnologists. Will > some of you Good Managers enlighten me as to why it's Good Management > Practice to have bench techs not know the cost of the items they work > with? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 02 Apr 2007 14:40:59 +1000 From: Cathy Malcontenti-Wilson Subject: [Histonet] Double immunostaining with a rabbit and rat primary antibody To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20070402142900.04427de8@mail.staff.unimelb.edu.au> Content-Type: text/plain; charset="us-ascii"; format=flowed Dear All, We wish to do a double immunostain for CD34 and active caspase 3 on paraformaldehyde fixed mouse tissues. The cd34 primary is rat monoclonal anti cd34 The caspase 3 primary is a rabbit polyclonal antibody. We would like to use the Dako Envision G/2 kit for double staining, however this kit is for mouse or rabbit primaries only. When we do a single stain for cd34, we use a Rabbit anti Rat IgG as a linking antibody between the primary and secondary antibody (Dako Envision plus kit for rabbit primaries) and this works well on our tissues in our experience. My question is: Can I add this linking antibody step to the method and still use the Envision G/2 kit? Does anyone have any experience with this sort of thing or any different ideas on doing double staining using these particular antibodies? All ideas and comment appreciated as always. Cathy ------------------------------ Message: 5 Date: Mon, 02 Apr 2007 10:20:54 +0300 From: Mikael Niku Subject: [Histonet] Prox1 / lymphatic endothelium To: histonet@lists.utsouthwestern.edu Message-ID: <4610AED6.1070208@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello! I'm looking for a marker for lymphatic endothelium, which should have wide species cross-reactivity, and works with paraffin-embedded, formalin-fixed sections. Chemicon/Upstate seems to have several promising products (two monos and one polyclonal) but there's little information available on these. Anyone have experience on these or other suitable antibodies? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mitdkv mieltd olen ldnsimaisesta sivistyksestd? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// ------------------------------ Message: 6 Date: Mon, 02 Apr 2007 12:09:43 +0200 From: "Marilyn Tyler" Subject: [Histonet] Eosinophils To: "histonet" Message-ID: <4610F287.A78E.0090.0@uct.ac.za> Content-Type: text/plain; charset=US-ASCII Hi Again Please could you tell me what tissue in mouse is a good positive control for eosinophil staining. Thanks Marilyn ------------------------------ Message: 7 Date: Mon, 2 Apr 2007 06:11:31 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" Subject: RE: [Histonet] Peloris processor To: "kristen arvidson" , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Please share with the whole group. Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, March 31, 2007 6:21 PM To: histonet Subject: [Histonet] Peloris processor Does anyone use the Peloris double chamber rapid tissue processor? What's it like? --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 02 Apr 2007 08:57:05 -0400 From: "Mildred Fail" Subject: [Histonet] Glycoprotein 2B, 3A To: , "Joyce Foster" Message-ID: Content-Type: text/plain; charset=US-ASCII Good morning, We are looking for a lab to do this test. thank you in advance for your help Rena Fail ------------------------------ Message: 9 Date: Mon, 2 Apr 2007 06:07:27 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Safety survey (2) To: histonet@lists.utsouthwestern.edu Message-ID: <953403.9514.qm@web61224.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Colleagues: Since 27 March when I posted about the "safety survey" 81 colleagues asked and received the questionnaire. So far 49 have been returned which, by all standards, is a very good response,but there are 32 still missing. To those of you who received the questionnaire and have not answered it yet, could you please fill it and send it back? The more answers I get the more representative the results will be. Thank you! Reni J. --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. ------------------------------ Message: 10 Date: Mon, 2 Apr 2007 14:11:07 +0100 From: "Leila Ramos" Subject: [Histonet] Help in brain tisuue processing To: histonet@lists.utsouthwestern.edu Message-ID: <8978a9cf0704020611qd9dc075pf85ab472e090bfea@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Mr. or Ms We are working in a small lab from Canarias Island (Spain). We work with goat brain with manual procedure, our problems are several in the processing: 1. We obtain very dry blocks, when we try to cut with the microtome, the tissues are fragile and it is impossible to obtain the correct tissue. 2. When "we get proper block" (we can cut better the tissue), then we try to put in the float, the tissue appears like a white colour in the slide. After when we finish the stain process (hematoxyline-eosina and kluver barrera both of them) the tissues are not attached on the glass slides. I explain you in fast way our protocol: 1. Fixation: the whole brain is embedded in Formol 10% (1 week minimum) 2. Dehydration: 80: etanol 30 minutes; 96: 30 min x 2 times; 100 : 30 min x 5 times; xylene 1 hour x 2 times 3. Tissue embedding in paraffin. In cassetes with pure paraffin all night- Only 1 step other times 2 changes in paraffin 1 hour 4. Cut with microtome at 8 um, slide with polylisine. 5. Stain process (Hematoxyline-Eosine and kluver barrera) Thank a lot!! Leila Ramos Instituto de Investigacisn y Ciencias de Puerto del Rosario ------------------------------ Message: 11 Date: Mon, 2 Apr 2007 09:53:22 -0400 From: "Judy Collins" Subject: [Histonet] Peloris Processor To: Message-ID: Content-Type: text/plain; charset="US-ASCII" We have the Peloris rapid tissue processor. It is xylene free but does not use microwaves. It uses isopropyl alcohol after the reagent alcohol to remove all traces of water and then goes straight to the paraffin from there. Amazingly, and contrary to everything we have been taught, this works! It processes small biopsies in approximately 1 hour. Shave biopsies take approximately 2 hours and the larger specimens 4 to 6 hours. We find the processing to be at least equal to routine processing and in many cases, superior to it. Because it does not use xylene, the tissues are not hard and brittle as they can sometimes be with routine processing. It has two separate retorts which can run two separate runs at the same time. We are able to process our specimens throughout the day instead of everything being processed at night as in the past. It has greatly improved our TAT. The Peloris tracks the number of blocks run and, based on this, tells you when to change the various solutions. As a result, we have saved a significant amount of money on reagents. We are extremely happy with ours. ------------------------------ Message: 12 Date: Mon, 02 Apr 2007 10:36:07 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] Help in brain tisuue processing To: Leila Ramos Cc: histonet@lists.utsouthwestern.edu Message-ID: <461114D7.5060402@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Greetings Leila If you are fixing the whole brain by immersion the interior morphology won't be very good. I suggest cutting the brain into 1 cm slices and fixing these for 1-2 weeks. You will be able to see enough of the anatomy to know the order of the slices. If you must process the brain whole you will need to fix by vascular perfusion to see much (any) of the interior anatomy and your processing will have to be much longer. The rest of your schedule does not allow enough time for dehydration, especially on a whole brain. The tissue does not stick to the slides because it was not properly dehydrated so the paraffin does not infiltrate. On the other hand, all night in hot paraffin is too long and produces brittle tissue. I suggest the following for 1 cm slices, not a whole goat brain. After fixation, wash in water for several hours or overnight. 50% ethanol 1-2 hours. Agitation of the tissue duringhis and subsequent steps is highly desirable. 70% ethanol 1-2 hours. 95% ethanol 1 hour. 100% ethanol 2 changes 1 hour each. 1:1 mix of 100% ethanol:xylene 1 hour. Xylene, 2 changes 1 hour each. 3 changes of melted paraffin, under vacuum if possible, 45 min each. Heat the wax only a few degrees above the melting point. Embed Leila Ramos wrote: > Dear Mr. or Ms > We are working in a small lab from Canarias Island (Spain). We work with > goat brain with manual procedure, our problems are several in the > processing: > > 1. We obtain very dry blocks, when we try to cut with the microtome, > the tissues are fragile and it is impossible to obtain the correct > tissue. > 2. When "we get proper block" (we can cut better the tissue), then we > try to put in the float, the tissue appears like a white colour in the > slide. After when we finish the stain process (hematoxyline-eosina and > kluver barrera both of them) the tissues are not attached on the glass > slides. > > I explain you in fast way our protocol: > > 1. Fixation: the whole brain is embedded in Formol 10% (1 week > minimum) > 2. Dehydration: 80: etanol 30 minutes; 96: 30 min x 2 times; 100 : 30 > min x 5 times; xylene 1 hour x 2 times > 3. Tissue embedding in paraffin. In cassetes with pure paraffin all > night- Only 1 step other times 2 changes in paraffin 1 hour > 4. Cut with microtome at 8 um, slide with polylisine. > 5. Stain process (Hematoxyline-Eosine and kluver barrera) > > Thank a lot!! > > Leila Ramos > Instituto de Investigacisn y Ciencias de Puerto del Rosario > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 13 Date: Mon, 02 Apr 2007 09:48:54 -0500 From: "Webb, Dorothy L" Subject: [Histonet] Biopsy bags To: Histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640B56@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" We use a fair amount of the biopsy bags and one can purchase them for half the price that was quoted in the last histo-net. We purchase ours from Thermo Fisher and still get them for $205.64 for 1000 bags. We do not use the blue sponges due to the fact that we noticed reagent carryover in our processor which compromised our quality of tissue processing. Also, we have found that if one does not make certain the mesh cassettes are "submerged" in formalin a piece of tissue may stick to the top of the cassette or in a corner and not get proper processing. That is why for now we have been utilizing the nylon bags and fine them easy and efficient to use. Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 14 Date: Mon, 2 Apr 2007 10:56:45 -0400 From: "Walzer Susan" Subject: [Histonet] FW: Job Opening To: "Histonet \(E-mail\)" Message-ID: <471953BC63077941B82C26A4338272B42F050D@ORLEV03.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" > -----Original Message----- > From: Walzer Susan > Sent: Wednesday, February 21, 2007 9:17 AM > To: Histonet (E-mail) > Subject: Job Opening > > We are looking for a histo-tech at St Pete. General Hospital. St Pete., FL . Apply at: > > http://www.stpetegeneralhospital.com/ > > > ------------------------------ Message: 15 Date: Mon, 2 Apr 2007 16:17:06 +0100 From: "Ian Montgomery" Subject: Fw: [Histonet] Eosinophils To: "Histonet" Message-ID: <004701c77539$fa0a1960$4724d182@ibls.gla.ac.uk> Content-Type: text/plain; charset="iso-8859-1" A mouse blood smear. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Marilyn Tyler" To: "histonet" Sent: Monday, April 02, 2007 11:09 AM Subject: [Histonet] Eosinophils > Hi Again > Please could you tell me what tissue in mouse is a good positive > control for eosinophil staining. Thanks > Marilyn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Mon, 02 Apr 2007 11:27:47 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] Eosinophils To: Marilyn Tyler Cc: histonet Message-ID: <461120F3.5030202@umdnj.edu> Content-Type: text/plain; format=flowed; charset=us-ascii Small intestine. Geoff Marilyn Tyler wrote: >Hi Again >Please could you tell me what tissue in mouse is a good positive >control for eosinophil staining. Thanks >Marilyn >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 17 Date: Mon, 2 Apr 2007 16:38:07 +0100 From: "Adam Kirk" Subject: [Histonet] Please unsubscribe me To: "histonet@lists.utsouthwestern.edu" Message-ID: <40e76ce80704020838t79db3be3tb9510420fe659a08@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Histonet, Please would you unsubscribe me from your mailing list? Thanks. Adam Kirk -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 ------------------------------ Message: 18 Date: Mon, 2 Apr 2007 11:43:33 -0400 (EDT) From: judi.ford@jax.org Subject: [Histonet] unsubscrible To: histonet@lists.utsouthwestern.edu Message-ID: <12180621.1175528613818.JavaMail.ocsadmin@jcs-mid-prod.jax.org> Content-Type: text/plain; charset=ISO-8859-1 Please unsubscribe me from the histonet list. Judi Ford HIstotechnologist ------------------------------ Message: 19 Date: Mon, 2 Apr 2007 18:11:57 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Peloris Processor and isopropanol To: Message-ID: <001801c77541$a382c990$6412a8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I've seen a protocol for isopropanol-processing with an IPA-temperature about 74 degrees. That is near the boilingpoint and means rather a boiling out of the tissue than acting as intermedium. Similar protocols are found with the microwave-techniques. I think there is no principal difference in reaching the temperature. What I'm a little confused about is the fact, that usually recommended temperatures for tissueprocessing don't raise above 62 degrees. So how does this influence staining, ihc-staining etc.? What are the temperatures in your protocol? What does the community think about this issue? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr|ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Judy Collins Gesendet: Montag, 02. April 2007 15:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Peloris Processor We have the Peloris rapid tissue processor. It is xylene free but does not use microwaves. It uses isopropyl alcohol after the reagent alcohol to remove all traces of water and then goes straight to the paraffin >from there. Amazingly, and contrary to everything we have been taught, this works! It processes small biopsies in approximately 1 hour. Shave biopsies take approximately 2 hours and the larger specimens 4 to 6 hours. We find the processing to be at least equal to routine processing and in many cases, superior to it. Because it does not use xylene, the tissues are not hard and brittle as they can sometimes be with routine processing. It has two separate retorts which can run two separate runs at the same time. We are able to process our specimens throughout the day instead of everything being processed at night as in the past. It has greatly improved our TAT. The Peloris tracks the number of blocks run and, based on this, tells you when to change the various solutions. As a result, we have saved a significant amount of money on reagents. We are extremely happy with ours. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Mon, 02 Apr 2007 12:15:14 -0400 From: "Maray Weirauch" Subject: [Histonet] Infectivity of FFPE tissue To: Message-ID: Content-Type: text/plain; charset=US-ASCII In the past, we have disposed of paraffin trimmings in regular trash and old or broken, stained and unstained slides in non-biohazard glass disposal unless they are from a suspected CJD case. We are reviewing this procedure and cannot find documentation to support our policy, in fact we have found some indication that TB may still be viable. What is everyone else doing for disposal of their paraffin debris and slides for disposal? Thanks for your input! ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 41, Issue 2 *************************************** From eileen_dusek <@t> yahoo.com Mon Apr 2 15:17:39 2007 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Mon Apr 2 15:17:44 2007 Subject: [Histonet] Cytology Prep job description Message-ID: <558276.44140.qm@web50603.mail.re2.yahoo.com> Hi Everyone, We are trying to create a new job at Edward Hospital and need a job description for Cyto Prep Techs. If anyone could share that with me I would truly apprecite your help. A lot of hospital seem to have Support Tech trained as Cyto Tech Prep but we want to go a step further. Please email if possible or fax to 630-527-3911. I appreciate your help. Eileen C. Dusek Edward Hospital Napervilel, Il 630-527-3545 --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From mtitford <@t> aol.com Mon Apr 2 15:45:34 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon Apr 2 15:45:56 2007 Subject: [Histonet] Alizarin red S for gunshot residue? Message-ID: <8C9437EFEDB2188-1A3C-8D30@FWM-D39.sysops.aol.com> A few days ago on the Histonet, there was an enquiry about using alizarin red S for demonstrating gunshot residue in hostologic sections. Has anyone else ever heard about this? How does it work? I would have thought gunshot residue would comprise of carbon and gunpowder/propellant, while alizarin stains calcium. Am I missing something? Michael Titford Pathology USA Mobile AL ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From bjdewe <@t> aol.com Mon Apr 2 15:53:29 2007 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Mon Apr 2 15:53:36 2007 Subject: [Histonet] Animal Tissue control slides Message-ID: <8C943801A2E1A4C-678-8CE9@WEBMAIL-RD12.sysops.aol.com> Please check out the new link to our animal tissue control slides: http://innvx.com/animalcontroltissueslides-ad.gif Let us know if you have any questions or suggestions ;-) Cheers, Loralei Dewe ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From bjdewe <@t> aol.com Mon Apr 2 16:31:10 2007 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Mon Apr 2 16:31:18 2007 Subject: [Histonet] FC receptor blocker Message-ID: <8C943855D943684-678-8EDB@WEBMAIL-RD12.sysops.aol.com> Check this out as well. This stuff works great! http://innvx.com/fc-ad.jpg Cheers, Loralei Dewe ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From mtarango <@t> nvcancer.org Mon Apr 2 19:50:31 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Apr 2 19:51:00 2007 Subject: [Histonet] Double immunostaining with a rabbit and rat primary antibody In-Reply-To: <6.2.1.2.2.20070402142900.04427de8@mail.staff.unimelb.edu.au> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6C60@NVCIEXCH02.NVCI.org> NO! Don't use the rabbit link. You need a mouse link. I'm pretty sure that kit works by having two different primary antibodies in DIFFERENT species (one in MOUSE and one in RABBIT). You already are using a rabbit primary for the caspase 3, so you need mouse now. If you want to link it up so the Dako kit that you have, will pick up the signal from the rat primary, then you need to turn that rat into a mouse! Use a mouse anti-rat antibody (doesn't have to conjugated to anything). Then you end up with the caspase 3 being rabbit and the CD34 being mouse. When you do this, you end up amplifying the signal, you might want to keep that in mind, but it probably doesn't matter. You just want to know if the cells are positive I'm guessing, since you're using polyclonal stuff anyway (polyclonals binding to various random epitopes would be tough to quantify, but you can find out if there is antigen being expressed). Make sure that the antibody you buy is highly cross-absorbed against other species... Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Malcontenti-Wilson Sent: Sunday, April 01, 2007 9:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Double immunostaining with a rabbit and rat primary antibody Dear All, We wish to do a double immunostain for CD34 and active caspase 3 on paraformaldehyde fixed mouse tissues. The cd34 primary is rat monoclonal anti cd34 The caspase 3 primary is a rabbit polyclonal antibody. We would like to use the Dako Envision G/2 kit for double staining, however this kit is for mouse or rabbit primaries only. When we do a single stain for cd34, we use a Rabbit anti Rat IgG as a linking antibody between the primary and secondary antibody (Dako Envision plus kit for rabbit primaries) and this works well on our tissues in our experience. My question is: Can I add this linking antibody step to the method and still use the Envision G/2 kit? Does anyone have any experience with this sort of thing or any different ideas on doing double staining using these particular antibodies? All ideas and comment appreciated as always. Cathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From c.m.vanderloos <@t> amc.uva.nl Tue Apr 3 02:20:51 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Apr 3 02:21:31 2007 Subject: [Histonet] RE: Double immunostaining with a rabbit and rat Message-ID: <261e7c3261fb57.261fb57261e7c3@amc.uva.nl> Hi Cathy, I cannot recommend you this G/2 kit as this is sequential as well as multi-species based. A home-brew solution is more appropriate here: Rt CD34 + Rb casp-3 (cocktail) Goat anti-rat/HRP (mouse absorbed)* + goat anti-rabbit/AP polymer ** (cocktail) Develop AP activity in red (Dako LPR) Develop HRP activity in brown (DAB+ from any source) * = see Jackson or Rockland ** = see LabVision Lots of success with staining! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 02 Apr 2007 14:40:59 +1000 From: Cathy Malcontenti-Wilson Subject: [Histonet] Double immunostaining with a rabbit and rat primary antibody To: histonet@lists.utsouthwestern.edu Dear All, We wish to do a double immunostain for CD34 and active caspase 3 on paraformaldehyde fixed mouse tissues. The cd34 primary is rat monoclonal anti cd34 The caspase 3 primary is a rabbit polyclonal antibody. We would like to use the Dako Envision G/2 kit for double staining, however this kit is for mouse or rabbit primaries only. When we do a single stain for cd34, we use a Rabbit anti Rat IgG as a linking antibody between the primary and secondary antibody (Dako Envision plus kit for rabbit primaries) and this works well on our tissues in our experience. My question is: Can I add this linking antibody step to the method and still use the Envision G/2 kit? Does anyone have ! any exper From gu.lang <@t> gmx.at Tue Apr 3 04:47:15 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 3 04:47:24 2007 Subject: AW: AW: [Histonet] Peloris Processor and isopropanol In-Reply-To: <46114B8F.9070005@ebsciences.com> Message-ID: <000901c775d5$109f2ee0$6412a8c0@dielangs.at> Yes, I've been told, that it works. But what is the histochemical background? Proper fixed tissue would'nt suffer too much from high temperatures, I think. What is about too thick, underfixed tissue? Wouldn't it become hard and tough, difficult to cut? What about shrinkage? What about collagen-rich tissue? I don't want to produce glue. Is the whole process sensitiv to the duration in the high temp.? What is the recommandation for the maximum duration in 80 degrees? What are the critical points? Many questions, I know. But I don't like black boxes. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Phil McArdle [mailto:pmcardle@ebsciences.com] Gesendet: Montag, 02. April 2007 20:30 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: AW: [Histonet] Peloris Processor and isopropanol I'm with a laboratory microwave vendor: Never mind 74? C - some microwave protocols call for temperatures as high as 82? or 84? C (!) in order to exceed the boiling point of isopropanol, facilitating its replacement with paraffin. Naturally, this caused some concern and resistance in the anatomic pathology community. However, look at your own question about IHC: when you consider antigen retrieval protocols call for 100?C, even 84? pales by comparison. Of course, there are many reasons you want to minimize the amount of time spent at high temperatures, but there is no problem with the majority of tissue types for the relatively short timeframes involved in microwave processing. Also, by the paraffin step, the tissue has already been fixed, dehydrated, and defatted; while it is in a sense still "tissue," it's radically different from the tissue as received. You certainly don?t want to burn or "cook" anything, but 82?C isn?t really extreme in this context. (I'd be at least as concerned about how a given paraffin and additives hold up at high temperatures, so it's always a good idea to check with the manufacturer.) There also is the issue of vacuum; since the purpose of high temperature is to get above the boiling point of isopropanol, and vacuum causes BP depression, vacuum should allow you to lower the temperature to something you're more comfortable with. More information may be found at http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf Very best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Gudrun Lang wrote: > I've seen a protocol for isopropanol-processing with an IPA-temperature > about 74 degrees. That is near the boilingpoint and means rather a boiling > out of the tissue than acting as intermedium. Similar protocols are found > with the microwave-techniques. I think there is no principal difference in > reaching the temperature. > > What I'm a little confused about is the fact, that usually recommended > temperatures for tissueprocessing don't raise above 62 degrees. So how does > this influence staining, ihc-staining etc.? > What are the temperatures in your protocol? > What does the community think about this issue? > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Judy > Collins > Gesendet: Montag, 02. April 2007 15:53 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Peloris Processor > > We have the Peloris rapid tissue processor. It is xylene free but does > not use microwaves. It uses isopropyl alcohol after the reagent alcohol > to remove all traces of water and then goes straight to the paraffin > >from there. Amazingly, and contrary to everything we have been taught, > this works! > > It processes small biopsies in approximately 1 hour. Shave biopsies > take approximately 2 hours and the larger specimens 4 to 6 hours. We > find the processing to be at least equal to routine processing and in > many cases, superior to it. Because it does not use xylene, the tissues > are not hard and brittle as they can sometimes be with routine > processing. > > It has two separate retorts which can run two separate runs at the same > time. We are able to process our specimens throughout the day instead > of everything being processed at night as in the past. It has greatly > improved our TAT. > > The Peloris tracks the number of blocks run and, based on this, tells > you when to change the various solutions. As a result, we have saved a > significant amount of money on reagents. > > We are extremely happy with ours. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From barrickstacey <@t> yahoo.com Tue Apr 3 08:39:49 2007 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Tue Apr 3 08:39:57 2007 Subject: [Histonet] Frozen sections falling off of slides Message-ID: <20070403133949.74774.qmail@web54301.mail.re2.yahoo.com> Hi everyone, I'm cutting frozen rat scg at 10um in OCT. I cannot keep the sections from falling off the slides during the overnight incubation in primary anitbody. I have tried the Superfrost plus slides and currently we are using the Superfrost Plus GOLD slides which are supposed to be even better. THis is what I've tried so far: Slides on warmer for 1hr Slides on warmer for 2hr Slides on warmer for 5hr Slides on warmer for 2hr, then at RT overnight Sometimes ALL sections fall off. Other times just some of the sections will fall off. When I remove them from the warmer, I cover them with PBS to rehydrate. THen incubate overnight in my primary antibody at 4deg C. The next morning is when I notice all of the floaters. THey are usually foggy when the come out of the fridge. When I put the slides into PBS most if not all of the sections are gone. Please help! This is precious tissue and I can't loose anymore. I've never had this problem with any other tissue I've used. I dont believe its been a problem in the past in this lab either but noone knows what the difference is between then and now. Also, what setting do you use on the slide warmer (temp?) I've tried setting it between 2 and 3 (usually 40deg C) Thanks in advance!! Stacey --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From dlcowie <@t> prodigy.net Tue Apr 3 09:39:22 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Tue Apr 3 09:39:31 2007 Subject: [Histonet] Frozen sections falling off of slides In-Reply-To: <20070403133949.74774.qmail@web54301.mail.re2.yahoo.com> Message-ID: <20070403143922.72225.qmail@web81009.mail.mud.yahoo.com> hi Stacey, Have you tried using Halt solution in your waterbath? We use a capful in each waterbath. It does not interfere with immunostaining like some of the other adhesives do. It might be worth a try. Dawn Stacey Barrick wrote: Hi everyone, I'm cutting frozen rat scg at 10um in OCT. I cannot keep the sections from falling off the slides during the overnight incubation in primary anitbody. I have tried the Superfrost plus slides and currently we are using the Superfrost Plus GOLD slides which are supposed to be even better. THis is what I've tried so far: Slides on warmer for 1hr Slides on warmer for 2hr Slides on warmer for 5hr Slides on warmer for 2hr, then at RT overnight Sometimes ALL sections fall off. Other times just some of the sections will fall off. When I remove them from the warmer, I cover them with PBS to rehydrate. THen incubate overnight in my primary antibody at 4deg C. The next morning is when I notice all of the floaters. THey are usually foggy when the come out of the fridge. When I put the slides into PBS most if not all of the sections are gone. Please help! This is precious tissue and I can't loose anymore. I've never had this problem with any other tissue I've used. I dont believe its been a problem in the past in this lab either but noone knows what the difference is between then and now. Also, what setting do you use on the slide warmer (temp?) I've tried setting it between 2 and 3 (usually 40deg C) Thanks in advance!! Stacey --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGarfield <@t> lifecell.com Tue Apr 3 10:13:36 2007 From: JGarfield <@t> lifecell.com (Jacqueline D. Garfield) Date: Tue Apr 3 10:13:58 2007 Subject: [Histonet] Frozen sections falling off of slides Message-ID: Hello Dawn, We occasionally have a similar problem with our paraffin sections for IHC. I haven't heard of "Halt solution". Do you use it for paraffin sections? If so where can I purchase it? Thank you, Jackie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Cowie Sent: Tuesday, April 03, 2007 10:39 AM To: Stacey Barrick; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen sections falling off of slides hi Stacey, Have you tried using Halt solution in your waterbath? We use a capful in each waterbath. It does not interfere with immunostaining like some of the other adhesives do. It might be worth a try. Dawn Stacey Barrick wrote: Hi everyone, I'm cutting frozen rat scg at 10um in OCT. I cannot keep the sections from falling off the slides during the overnight incubation in primary anitbody. I have tried the Superfrost plus slides and currently we are using the Superfrost Plus GOLD slides which are supposed to be even better. THis is what I've tried so far: Slides on warmer for 1hr Slides on warmer for 2hr Slides on warmer for 5hr Slides on warmer for 2hr, then at RT overnight Sometimes ALL sections fall off. Other times just some of the sections will fall off. When I remove them from the warmer, I cover them with PBS to rehydrate. THen incubate overnight in my primary antibody at 4deg C. The next morning is when I notice all of the floaters. THey are usually foggy when the come out of the fridge. When I put the slides into PBS most if not all of the sections are gone. Please help! This is precious tissue and I can't loose anymore. I've never had this problem with any other tissue I've used. I dont believe its been a problem in the past in this lab either but noone knows what the difference is between then and now. Also, what setting do you use on the slide warmer (temp?) I've tried setting it between 2 and 3 (usually 40deg C) Thanks in advance!! Stacey --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From Heather.D.Renko <@t> osfhealthcare.org Tue Apr 3 10:19:49 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Apr 3 10:19:59 2007 Subject: [Histonet] slide and cassette labeler Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775404@pmc-rfd-mx01.intranet.osfnet.org> Are there any labs out there with a good LIS slide and cassette labeler that interfaces well with Copath? Please send me what is working well for you. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From dlcowie <@t> prodigy.net Tue Apr 3 11:04:11 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Tue Apr 3 11:04:19 2007 Subject: [Histonet] Frozen sections falling off of slides In-Reply-To: Message-ID: <402465.1472.qm@web81002.mail.mud.yahoo.com> Jackie, You can purchase Halt solution from Poly Scientific. Catalog number: S2430. comes in 16oz bottle for $30.50 each. Yes, we do use it for paraffin sections. Put 1 capful in your waterbath. Dawn "Jacqueline D. Garfield" wrote: Hello Dawn, We occasionally have a similar problem with our paraffin sections for IHC. I haven't heard of "Halt solution". Do you use it for paraffin sections? If so where can I purchase it? Thank you, Jackie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Cowie Sent: Tuesday, April 03, 2007 10:39 AM To: Stacey Barrick; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen sections falling off of slides hi Stacey, Have you tried using Halt solution in your waterbath? We use a capful in each waterbath. It does not interfere with immunostaining like some of the other adhesives do. It might be worth a try. Dawn Stacey Barrick wrote: Hi everyone, I'm cutting frozen rat scg at 10um in OCT. I cannot keep the sections from falling off the slides during the overnight incubation in primary anitbody. I have tried the Superfrost plus slides and currently we are using the Superfrost Plus GOLD slides which are supposed to be even better. THis is what I've tried so far: Slides on warmer for 1hr Slides on warmer for 2hr Slides on warmer for 5hr Slides on warmer for 2hr, then at RT overnight Sometimes ALL sections fall off. Other times just some of the sections will fall off. When I remove them from the warmer, I cover them with PBS to rehydrate. THen incubate overnight in my primary antibody at 4deg C. The next morning is when I notice all of the floaters. THey are usually foggy when the come out of the fridge. When I put the slides into PBS most if not all of the sections are gone. Please help! This is precious tissue and I can't loose anymore. I've never had this problem with any other tissue I've used. I dont believe its been a problem in the past in this lab either but noone knows what the difference is between then and now. Also, what setting do you use on the slide warmer (temp?) I've tried setting it between 2 and 3 (usually 40deg C) Thanks in advance!! Stacey --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From amylee779 <@t> yahoo.com Tue Apr 3 11:35:19 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Tue Apr 3 11:35:26 2007 Subject: [Histonet] Sakura embedding station TEC 5 Message-ID: <733673.5382.qm@web38004.mail.mud.yahoo.com> Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From dlcowie <@t> prodigy.net Tue Apr 3 13:05:02 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Tue Apr 3 13:05:10 2007 Subject: [Histonet] Sakura embedding station TEC 5 In-Reply-To: <733673.5382.qm@web38004.mail.mud.yahoo.com> Message-ID: <382174.76486.qm@web81007.mail.mud.yahoo.com> Amy, The embedding station I like to work with the best is the Leica EG1160. We purchased a Sakura TEC5. The one quirk we noticed is that you cannot program it to come on before midnight and go off the next morning. For example, we start embedding at 10:30pm and wanted it to go off at 9am the next day. If it's before midnight it won't let you program it this way. Sakura doesn't have an answer for this. Otherwise, its a good instrument. Dawn Amy Lee wrote: Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Yves.Heremans <@t> vub.ac.be Tue Apr 3 13:11:51 2007 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Tue Apr 3 13:12:01 2007 Subject: [Histonet] double-staining with two rabbit antibodies, Zenon-kit Message-ID: <20070403181151.020EA532@bonito.ulb.ac.be> Dear All, We need to double-stain with two antibodies made in rabbit and would prefer to use fluorescent detection for both. I know that Molecular Probes has Zenon kits for differential fluorescent labeling of rabbit antibodies, allowing you to use multiple antibodies from the same species, however these kits require that you know the concentration of the antibodies to be labeled. Since our rabbit antibodies are not affinity-purified but consist of whole serum, is there a way around this and use the Zenon kit anyway ? Yves From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Apr 3 13:38:08 2007 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Apr 3 13:38:19 2007 Subject: [Histonet] Binding pathology reports Message-ID: Hi, I'd like to know how many of you have your pathology reports bound into "book form". Is there a requirement to do this? My reason for asking- our pathology secretary insists this needs to be done, she says all hospitals she knows do this. It is taking up lots of storage space. All of the reports are in our computer system. Thanks for your help! Stacy McLaughlin From dusko.trajkovic <@t> pfizer.com Tue Apr 3 13:44:52 2007 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Tue Apr 3 13:45:00 2007 Subject: [Histonet] Sakura embedding station TEC 5 In-Reply-To: <733673.5382.qm@web38004.mail.mud.yahoo.com> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B20431151D@lajamrexm01.amer.pfizer.com> We have a Sekura TEC 5 and a Leica. Everyone (4 colleagues) prefers the Sekura. On Sakura, paraffin, cassette, and embedding surface are on all of the time. Whenever we need to embed, the cryo chamber is turned on and once the work is done, cryo is turned off. Try doing that with a Leica. Either everything is on, or everything is off. Smart engineering. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, April 03, 2007 9:35 AM To: histonet Subject: [Histonet] Sakura embedding station TEC 5 Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From Heather.D.Renko <@t> osfhealthcare.org Tue Apr 3 14:04:01 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Apr 3 14:04:04 2007 Subject: [Histonet] negatives Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612CCA@pmc-rfd-mx01.intranet.osfnet.org> Histo netters: I got a call from a fellow tech asking me if I run a negative control on my special stains and my H&E slides. I was completely stumped because I have always used my internal negative and an a positive for each stain. My explanation is that the positive is for the test and the negative is for the patient. I have only run negative for higher testing such as IHC, ISH, etc. Can someone help me to help my fellow tech with the explanation. Nancy washkowiak, Ottawa Community Hospital 'nwash_24@yahoo.com' Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From AGrobe2555 <@t> aol.com Tue Apr 3 14:05:18 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Apr 3 14:05:32 2007 Subject: [Histonet] double-staining with two rabbit antibodies, Zenon-kit Message-ID: Yves, Just do the complete stain for one (block, rabbit primary#1, fluorescent secondary#1), block well and then do the second (rabbit primary#2, fluorescent secondary#2). Use two different color anti-rabbit fluorophores. I have done this in the past with no apparent problems. Another option is to pre-react the two primaries with different anti-rabbit fluorophores in separate tubes, and then add to the section. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From lpaveli1 <@t> hurleymc.com Tue Apr 3 14:10:16 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Apr 3 14:10:41 2007 Subject: [Histonet] Sakura embedding station TEC 5 Message-ID: <46126E59020000EE00012E44@smtp-gw.hurleymc.com> Amy, Our hospital has a Leica EG1040H (hot plate) and a EG1040C (cold plate). These two units are separate. This makes it cheaper to replace one if one or the other 'dies'. You have the option on the heating side to program what time you want it to start heating, what days to heat, or the option to always have it "on". The cold side has a "on" or "off" switch. We like the equipment. They have made some very good changes with the wax collection drawer on the heat side and we are very happy. hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Trajkovic, Dusko" 04/03/07 2:44 PM >>> We have a Sekura TEC 5 and a Leica. Everyone (4 colleagues) prefers the Sekura. On Sakura, paraffin, cassette, and embedding surface are on all of the time. Whenever we need to embed, the cryo chamber is turned on and once the work is done, cryo is turned off. Try doing that with a Leica. Either everything is on, or everything is off. Smart engineering. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, April 03, 2007 9:35 AM To: histonet Subject: [Histonet] Sakura embedding station TEC 5 Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Apr 3 14:12:08 2007 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Apr 3 14:12:10 2007 Subject: [Histonet] Grosslab operation instructions Message-ID: Hi, Does anyone out there in Histoland have the operation instructions for a Grosslab model 87001? If so- could you please e-mail me off line? Thanks a bunch! Stacy McLaughlin Cooley Dickinson Hospital From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Apr 3 14:20:03 2007 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Apr 3 14:20:01 2007 Subject: [Histonet] Binding Pathology reports-THANK YOU!! Message-ID: Hi Everyone, Just wanted to send a big THANK YOU to all of you for your responses! You have been very helpful. Sometimes getting things changed is like pulling teeth! :-) Stacy McLaughlin From gu.lang <@t> gmx.at Tue Apr 3 14:54:50 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 3 14:55:02 2007 Subject: AW: [Histonet] negatives In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C06612CCA@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <000901c77629$f198ad00$6412a8c0@dielangs.at> I am curious what material is used for making a negative HE-control? Glass? In my opinion positive and negative controls make only sense with stains that give a yes/no-result, like ZN. With the other stains I think it is rather obvious, if they have worked or not. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Renko, Heather D. Gesendet: Dienstag, 03. April 2007 21:04 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] negatives Histo netters: I got a call from a fellow tech asking me if I run a negative control on my special stains and my H&E slides. I was completely stumped because I have always used my internal negative and an a positive for each stain. My explanation is that the positive is for the test and the negative is for the patient. I have only run negative for higher testing such as IHC, ISH, etc. Can someone help me to help my fellow tech with the explanation. Nancy washkowiak, Ottawa Community Hospital 'nwash_24@yahoo.com' Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCazares <@t> schosp.org Tue Apr 3 15:24:01 2007 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Tue Apr 3 15:24:13 2007 Subject: [Histonet] negatives In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C06612CCA@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C06612CCA@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <913FAC2B773C19488E26AE6572180FA509BF0170@exch01.schosp.org> I have looked through the CAP checklist that I have just received for our self inspection and the only section where negative controls are mentioned is in the IHC section. Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Tuesday, April 03, 2007 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negatives Histo netters: I got a call from a fellow tech asking me if I run a negative control on my special stains and my H&E slides. I was completely stumped because I have always used my internal negative and an a positive for each stain. My explanation is that the positive is for the test and the negative is for the patient. I have only run negative for higher testing such as IHC, ISH, etc. Can someone help me to help my fellow tech with the explanation. Nancy washkowiak, Ottawa Community Hospital 'nwash_24@yahoo.com' Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From gcallis <@t> montana.edu Tue Apr 3 17:14:44 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 3 17:14:46 2007 Subject: [Histonet] frozen sections falling off slides Message-ID: <6.0.0.22.1.20070403161035.01b4e9c0@gemini.msu.montana.edu> Stacey, What I am not seeing here is if your tissues are prefixed with formalin or similar fixative before you do the cryosections, or are you working with unfixed tissue frozen sections? Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 You wrote: I'm cutting frozen rat scg at 10um in OCT. I cannot keep the sections from falling off the slides during the overnight incubation in primary anitbody. I have tried the Superfrost plus slides and currently we are using the Superfrost Plus GOLD slides which are supposed to be even better. THis is what I've tried so far: Slides on warmer for 1hr Slides on warmer for 2hr Slides on warmer for 5hr Slides on warmer for 2hr, then at RT overnight Sometimes ALL sections fall off. Other times just some of the sections will fall off. When I remove them from the warmer, I cover them with PBS to rehydrate. THen incubate overnight in my primary antibody at 4deg C. The next morning is when I notice all of the floaters. THey are usually foggy when the come out of the fridge. When I put the slides into PBS most if not all of the sections are gone. Please help! This is precious tissue and I can't loose anymore. I've never had this problem with any other tissue I've used. I dont believe its been a problem in the past in this lab either but noone knows what the difference is between then and now. Also, what setting do you use on the slide warmer (temp?) I've tried setting it between 2 and 3 (usually 40deg C) Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From alanb <@t> medlabcentral.co.nz Tue Apr 3 17:56:42 2007 From: alanb <@t> medlabcentral.co.nz (Alan Bishop) Date: Tue Apr 3 18:04:47 2007 Subject: [Histonet] Sakura embedding station TEC 5 In-Reply-To: <382174.76486.qm@web81007.mail.mud.yahoo.com> Message-ID: couldn't you just fool it by changing AM to PM? Therefore it thinks it's turning on at 10.30am and off at 9pm? Cheers Alan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawn Cowie Sent: Wednesday, April 04, 2007 6:05 AM To: Amy Lee; histonet Subject: Re: [Histonet] Sakura embedding station TEC 5 Amy, The embedding station I like to work with the best is the Leica EG1160. We purchased a Sakura TEC5. The one quirk we noticed is that you cannot program it to come on before midnight and go off the next morning. For example, we start embedding at 10:30pm and wanted it to go off at 9am the next day. If it's before midnight it won't let you program it this way. Sakura doesn't have an answer for this. Otherwise, its a good instrument. Dawn Amy Lee wrote: Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 2167 (20070403) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From dlcowie <@t> prodigy.net Tue Apr 3 18:31:31 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Tue Apr 3 18:31:36 2007 Subject: [Histonet] Sakura embedding station TEC 5 In-Reply-To: Message-ID: <538749.20974.qm@web81011.mail.mud.yahoo.com> I never thought of that. I definitely must try harder to be smarter than my instruments. ha ha. Dawn Alan Bishop wrote: couldn't you just fool it by changing AM to PM? Therefore it thinks it's turning on at 10.30am and off at 9pm? Cheers Alan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawn Cowie Sent: Wednesday, April 04, 2007 6:05 AM To: Amy Lee; histonet Subject: Re: [Histonet] Sakura embedding station TEC 5 Amy, The embedding station I like to work with the best is the Leica EG1160. We purchased a Sakura TEC5. The one quirk we noticed is that you cannot program it to come on before midnight and go off the next morning. For example, we start embedding at 10:30pm and wanted it to go off at 9am the next day. If it's before midnight it won't let you program it this way. Sakura doesn't have an answer for this. Otherwise, its a good instrument. Dawn Amy Lee wrote: Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 2167 (20070403) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jqb7 <@t> cdc.gov Wed Apr 4 05:28:44 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 4 05:28:50 2007 Subject: [Histonet] Sakura embedding station TEC 5 References: <46126E59020000EE00012E44@smtp-gw.hurleymc.com> Message-ID: The Sakura also has a 2-module design. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Tuesday, April 03, 2007 3:10 PM To: histonet@lists.utsouthwestern.edu; dusko.trajkovic@pfizer.com; amylee779@yahoo.com Subject: RE: [Histonet] Sakura embedding station TEC 5 Amy, Our hospital has a Leica EG1040H (hot plate) and a EG1040C (cold plate). These two units are separate. This makes it cheaper to replace one if one or the other 'dies'. You have the option on the heating side to program what time you want it to start heating, what days to heat, or the option to always have it "on". The cold side has a "on" or "off" switch. We like the equipment. They have made some very good changes with the wax collection drawer on the heat side and we are very happy. hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Trajkovic, Dusko" 04/03/07 2:44 PM >>> We have a Sekura TEC 5 and a Leica. Everyone (4 colleagues) prefers the Sekura. On Sakura, paraffin, cassette, and embedding surface are on all of the time. Whenever we need to embed, the cryo chamber is turned on and once the work is done, cryo is turned off. Try doing that with a Leica. Either everything is on, or everything is off. Smart engineering. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, April 03, 2007 9:35 AM To: histonet Subject: [Histonet] Sakura embedding station TEC 5 Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlafrini <@t> csmlab.com Wed Apr 4 07:03:14 2007 From: mlafrini <@t> csmlab.com (Michael LaFriniere) Date: Wed Apr 4 07:03:58 2007 Subject: [Histonet] Maryland Histo/Cyto positions Message-ID: <46135B93.5898.00AF.0@csmlab.com> Cytology and Histology Services (CSM) Maryland is a CAP accredited private A/P laboratory owned by Adventist HealthCare in Maryland. Our primary customers are 4 large hospital clients in the DC and Maryland vicinity, In addition to private physician offices/surgery centers and public health provider for pathology services to several adjacent States. We are expanding! Our site in Laurel Maryland is currently seeking to fill new positions: Afternoon Lab Manager: HT (ASCP) with management experience in addition, some grossing (biopsies) experience preferred, hours are flexible. HT(ASCP) afternoons and midnight shifts available. CT(ASCP) full time and part time positions available. Great Benefits and salary! Feel free to contact me through email or direct! No head hunters need to respond to this email please! Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com ----------------------------------------- From failm <@t> musc.edu Wed Apr 4 08:25:01 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Wed Apr 4 08:25:14 2007 Subject: [Histonet] CD61 Message-ID: Good morning, Does anyone know of a lab that can stain for this marker. Thanks in advance for your help Rena Fail From LSebree <@t> uwhealth.org Wed Apr 4 09:01:35 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Apr 4 09:01:42 2007 Subject: [Histonet] CD61 In-Reply-To: Message-ID: US Labs, (800) 710-1800, in CA does CD61. They are our "go to" reference lab and we highly recommend them. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mildred Fail Sent: Wednesday, April 04, 2007 8:25 AM To: histonet@lists.utsouthwestern.edu; Joyce Foster Subject: [Histonet] CD61 Good morning, Does anyone know of a lab that can stain for this marker. Thanks in advance for your help Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Wed Apr 4 09:05:44 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Apr 4 09:06:09 2007 Subject: [Histonet] Missouri Society for Histotechnology - Annual Meeting - May 31, June 1-2, 2007 - The Lodge of the Ozarks, Branson, Missouri Message-ID: On behalf of the organizing committee for the Missouri Society for Histotechnology Symposium To be held in Branson, Missouri on May 31, June 1-2 We invite you to participate in a unique learning experience. The program is outstanding this year and should have something for everyone. In addition there will be social activities and vendor exhibits. We look forward to seeing you. Sharon Ann Walsh-Conference Coordinator: userwalsh@sbcglobal.net Rosetta Barkley-Registration Coordinator: Rbarkley2@kumc.edu Jean-Philippe REY-Exhibit Coordinator: jpr@stowers-institute.org Contact one of the above for a .pdf of the registration form if you need one. Forms will also be available through the NSH region V area at nsh.org (currently in process). ---General Information:--- Meeting Location: Lodge of the Ozarks 3431 West Hwy 76 Branson, MO 65616 Phone (877) 337-5474 www.lodgeoftheozarks.com Hotel Information: Room reservations should be made directly with the Lodge of the Ozarks, Branson, MO . The toll free number is 1-800-213-2584. Hotel reservation deadline is Tuesday, May 1, 2007. To secure your symposium rates, make your reservations early and indicate that you are attending the MSH symposium. Room Rates: $65.00 plus 4% tax for single/double Check in 3:00 pm (can request early check in if available) Check out 11:00 am Lodge of the Ozarks is a premiere property centrally located on the famous Hwy 76 "Strip" within walking distance of countless shows, shopping, restaurants, and attractions - including the new Titanic Museum! You will be impressed with the Club Vegas Lounge & Supper Club, as well as the Timber Creek Cafe! ll you need is right here - no wonder they are called The Jewel of Branson! Complimentary continental express breakfast vouchers provided to all guests upon check-in. Rooms include coffee makers, hair dryers, irons & ironing boards. Jacuzzi rooms available at additional cost. Microwave and refrigerators (select rooms). Wi-Fi internet throughout hotel, including in-room. Free parking and children under 16 stay free. -----Schedule:------- All workshops are CEU approved! -------- Thursday, May 31, 2007 -------- 5:30-9:00 Registration 6:00-7:00 Kick-off Seminar - Instrument Maintenance & Repair Stanley Weglarz, Medical Bio Technologies, LLC. 7:00-9:00 Vendor Reception - Courtesy of MSH in Exhibit Hall TBS Hospitality Suite (Thursday Evening) -------- Friday, June 1, 2007 -------- 7:00 - 8:00 Registration 8:15 - 8:30 President Message - Sharon Ann Walsh 8:30 -12:00 Scientific Session #1 >From Collection to Management: Techniques for the Management of Small Tissue Specimens. James McCormick M.D., Lee Dickey, H. Skip Brown, M.Div., HT(ASCP), Drew Mehta, McCormick Scientific, St. Louis, MO 10:00 -11:00EXHIBIT HALL OPEN 12:00 -1:00 AWARDS LUNCHEON: Region V Update, Konnie Zeitner, HT (ASCP), HTL, SLS, Region V Director 1:00 - 4:30 Workshop #1 Sponsored by Biocare Medical Immunohistochemistry Mathematics in the Laboratory Joel Martinez, BS, Biocare Medical, Walnut Creek, CA Workshop #2 Sponsored by McCormick Scientific Family Affair- Systems Approach to Team Development H. Skip Brown, M.Div., HT(ASCP), McCormick Scientific, St. Louis, MO Workshop #3 Sponsored by Dako Basic Immunohistochemistry & More Pam Hesch HT,HTL,(ASCP)QIHC, Technical Representative, Dako North America, Carpinteria, CA 3:00 - 3:30 EXHIBIT HALL OPEN ------ Saturday, June 2, 2007 -------- 7:00 - 8:00 Registration 8:00 - Noon Scientific Session #2 8:15 - 9:00 Birth of a Protein Jean-Philippe REY, M.S., Kansas City, MO 9:00 - 10:00 Basic Dynamics of Fixation and Processing H. Skip Brown, M.Div., HT( SCP) McCormick Scientific, St. Louis, MO 10:00 - 10:30 EXHIBIT HALL OPEN 10:30 - 11:30 Moh's Micrographic Surgery. What is it? Lisa Jackson, BS HT(ASCP) St. Louis University Medical Center, St. Louis, MO 11:30 - 1:00 EXHIBIT HALL OPEN - Snack Break 8:00 - 11:30 Scientific Session 3 Sponsored by Lab Storage Systems, Inc., St. Peters, MO Practical Approach to Immunohistochemical Stain Panels (aka "Oh @#$%! Dr. Mathur's signing out---it's gonna be a long day on immunos!") Sharad Mathur, M.D., VAMC, Kansas City, MO 1:00 - 4:30 Workshop #4 Sponsored by Vision BioSystems Where Do I Find that Immuno antibody or Reagent and what Do I Do with it once I have it? Charlie Dorner, HT (ASCP) Workshop #5 Sponsored by Ortho-Clinical Diagnostics How "Lean" Principles Can Be Applied to the Histology Lab Janice Mahoney, HT(ASCP) Alegent Health, Omaha, Nebraska, Ortho Workshop #6 Lab Safety: "It's Mostly a Chemical Thing or How not to Experience the Big Bang" Konnie Zeitner, HT(ASCP HTL, SLS, Nebraska Medical Center, Omaha, NE === Workshop Descriptions === Workshop#1 (Sponsored by Biocare Medical) Immunohistochemistry Mathematics in the Laboratory- Joel Martinez, BS, Biocare Medical As the field of immunohistochemistry continues to evolve and grow for both clinical and research laboratories, so does cost. Concentrated antibodies can be tedious to workup, so this hands-on workshop will demonstrate dilutions, appropriate diluents, storage and needed supplies, Diluents play a very important role in optimal staining, immunoassays with different diluents will be preformed. The importance of pH in the laboratory will also be discussed along with the titration of pH. Determining cost per slide for IHC reagents will also be demonstrated and actual mathematics scenarios will be setup and worked up. Workshop #2 (Sponsored by McCormick Scientific) A Family Affair-A Systems Approach to Team Development H. Skip Brown,M.Div,HT(ASCP), McCormick Scientific With the diversities in individual personalities, goals, and priorities, as well as internal and external influences to a group, the question is raised, 'what are the key elements that first, binds teams together, then creates a wholesome healthy environment where the members within function and relate to one another in alignment with unified goals.' One of the most dynamic examples of team development and maintenance is that of the social sciences model of family systems. Using a systems approach, this workshop will discuss the factors involved in developing team structure, the individual influence that each member has on the team and the value of their contribution in the overall system. The concept of 'Mobile Equilibrium', will be used to show students the symbiosis that exists between members of a system that help maintain a homeostatic environment. Also discussed will be the effects of conflict within the system, the imbalance that it presents to the equilibrium, and methods to address and bring the environment back into positive alignment with goals. By the end of the class participants will understand that in any team or group system, there are multiple dynamics within the group, all of which are affected in some way by change. One then can begin to predict reactions and more successfully manage the individual skills, talents, and behaviors that each member contributes to the team. Workshop #3 (Sponsored by Dako) Basic Immunohistochemistry & More Pam Hesch HT,HTL(ASCP) Dako Technical Representative This workshop addresses five areas of Immunohistochemistry that will help you be successful. We will discuss the impact of fixation, monoclonal vs. polyclonal antibodies, pretreatment of slides and identifying background staining. Workshop includes strategies for adding new antibodies to your lab. Workshop #4 (Sponsored by Vision BioSystems) Where do I find that Immuno Antibody or Reagent and What do I do with it once I have it ? -Charlie Dorner HT(ASCP),Vision BioSystems In the ever changing world of IHC, there are new antibodies and clones being developed everyday. This seminar will cover the various ways of locating and obtaining antibodies and different formats that they are available in. Participants will learn what to do with this new antibody once it is in their lab including optimization, troubleshooting, deciding on proper control and testing materials, and recording keeping. Additional discussion will center on choosing monoclonal or polyclonal antibodies, picking the right detection kit, and the use of additional blocking or enhancements. Upon completion of this course, the participants will have ideas to aide in the structure of an antibody development program for their facility. Workshop #5 (Sponsored by Ortho-Clinical Diagnostics) How "LEAN" Principles can be Applied to the Histology Lab Janice Mahoney ,HT(ASCP),Alegent Health Center, Omaha, NE The laboratory world is all-abuzz with the term "LEAN". What impact might Lean Principles have on the Histology Laboratory? In today's healthcare world, competition and innovations are driving lab leaders to seek ways to boost performance and increase productivity. LEAN is a proven message used to maximize productivity, reduce turn around times, and reduce cost utilizing standard processes. Because most of the processes in the anatomic pathology lab are manual, many feel Lean has little application. Learn how one laboratory took the Principles of LEAN used from manufacturing and applied them to the histology & cytology lab. This seminar will teach the benefits of single piece flow, standard work, lab layout and design. There will be some discussion of how LEAN influences the selection of laboratory equipment. LEAN processes are very different from the processes we have used to perform our work for many years. This major change and reframing of how we think about our jobs can be difficult for many people. This seminar will also include lesions learned to help staff deal with change and be part of the process of change. If you want to learn methods to get your slides to the Pathologists sooner, reduce cost, and increase productivity without over burdening the techs in your lab, this seminar will teach you some new principles that will make it possible. Workshop #6 Lab Safety: "It's Mostly a Chemical Thing or How not to Experience the Big Bang", - Konnie Zeitner, HT,HTL(ASCP),SLS, Nebraska Medical Center, Omaha, NE Laboratory safety is not only a good practice, it is required by federal regulations. This presentation will include the Federal Stands that impact our laboratory practice, a practical approach to implementing the regulations and information that can make your lab a safe place to work. Scientific Session #3 (Sponsored by Lab Storage Systems, Inc.) A Practical Approach to Immunohistochemical Stain Panels (aka "Oh @#$%! Dr. Mathur's signing out---it's going to be a long day on immunos!" -Sharad C. Mathur, M.D., VAMC, Kansas City, MO The workshop will discuss the rationales for using judicious panels of immunohistochemical stains to improve diagnostic yield. Specific examples of panels and the associated diagnostic algorithms will be presented. Practical aspects related to choice of antibodies and handling of small biopsies will be discussed. From dlcowie <@t> prodigy.net Wed Apr 4 09:28:26 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Wed Apr 4 09:28:36 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC304B163E5@NCH01EX02.nch.org> Message-ID: <67456.5001.qm@web81010.mail.mud.yahoo.com> Laura, Thank you so much for the detailed info. I will keep this in mind if we decide to try microwaving again (we probably will, its just a question of when). If I'm ever up in that area I'll contact you and see if we can arrange a visit. I understand you being busy, its the same here. There never seems to be enough hours in the day to get everything you need done. My list of "to do" things doesn't seem to get shorter. Dawn "Galiotto, Laura" wrote: Hello Dawn, This microwave system can process tissue as thick as 5 mm , does not restrict reagent use (buying reagents from them only leads to increase in cost), it is versatile and has many other programs such as rapid decal, gross hardening and lymph node detection using saline, and gives the freedom of modifying programs. I purchase the same reagents I have always used. I continue to fix in 10%NBF, this is a requirement for FDA approved testing. I have modified the programs to meet our needs, this includes ambient fixation in formalin prior to rapid fixation allowing us to put fresh tissue on it. I am getting a second unit so I may stagger start times and use the other options to help create a safer lab and improve time and quality on case. My next phase is to process breast cores that are thicker than 3mm and bone marrow core biopsies same day. When I was in the market to purchase our first unit, I discovered this system had more flexibility than other units, others had restricts to size, where we purchase the reagent from and they did not have the versatile use such as rapid decal. We are located in the northwest suburbs of Chicago, near the Arlington Horse Race Track. If you give me a couple days notice I will make sure my schedule is clear. I spend a lot of time in meetings. Laura Galiotto, PBT,HT(ASCP) Histology Manager Northwest Community Hospital Arlington Heights, Il 60056 847-618-6190 -----Original Message----- From: Dawn Cowie [mailto:dlcowie@prodigy.net] Sent: Saturday, March 31, 2007 5:27 AM To: Galiotto, Laura; histonet Subject: RE: [Histonet] delivery time of last tray of H&E Hello Laura, Thanks for the info on the RHS. What do you mean by "limitless system"? Where is your lab located? I would like to see another lab running this microwave. Thanks, Dawn "Galiotto, Laura" wrote: Hello Dawn, We have the RHS-1 and are getting a second one. I like the limitless system. We are processing tissue as thick as 3mm starting the run at 10:30 and handing in the slides at 16:00. It also does not restrict you to a different reagent or vendor for reagents. I continue to use 10%NBF for fixation and ethanol & Isopropanol for dehydration, then immediately into paraffin. If you are ever in town stop by to see it. Thanks Laura -----Original Message----- From: Dawn Cowie [mailto:dlcowie@prodigy.net] Sent: Friday, March 23, 2007 10:11 AM To: Galiotto, Laura Subject: RE: [Histonet] delivery time of last tray of H&E Laura, It is definitely hard to find certified techs willing to do those hours. I'm lucky at the moment - the techs that work these hours want to. I'm not sure what we'll do when 1 of them retires probably in the next couple of years. What kind of microwave processor do you run? We are going to look at this again. We tried the RHS-1 a few years ago- docs didn't like results - too labour intensive for the techs. thanks for your response, Dawn "Galiotto, Laura" wrote: Hello Dawn, Yeah, however you do bring up a very valid point. I do get the specialist, ie.. oncologist,pulmonaryologist, that come in very early to check slides before the pathologist gets in. My biggest problem is finding certified technicians that will work the night shift, practically non-existant. Thank goodness for our microwave processor. We now are processing most biopsies sameday. Laura Galiotto, HT(ASCP) Histology Manager --------------------------------- From: Dawn Cowie [mailto:dlcowie@prodigy.net] Sent: Fri 3/23/2007 3:03 AM To: Douglas D Deltour; 'Marshall Terry Dr, Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E We do what we are required to do by the pathologists. I don't know why they think they need all H&E's so early - they can't look at them all at the same time and sign them out - except that they have them just in case a clinician calls. The trend these days is certainly toward faster and faster turnaround time. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, Florida 32503 850-416-7251 Douglas D Deltour wrote: I don't get it either. We have a little histo pixies that comes in while we sleep and the slides magically appear on the pathologist desk in the morning. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! 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From kgrobert <@t> rci.rutgers.edu Wed Apr 4 09:43:35 2007 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Apr 4 09:39:24 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <67456.5001.qm@web81010.mail.mud.yahoo.com> References: <67456.5001.qm@web81010.mail.mud.yahoo.com> Message-ID: <4613B997.9070006@rci.rutgers.edu> Dawn- That is the story of EVERY technician, not just histo techs. :o) I am a lab tech here at Rutgers who learned how to section and stain (both H&E and specials, plus some IHC every now and then) as part of my duties, and between that and everything else-running the lab, teaching, maintenance (both lab and computer), plus my own graduate research, my list rarely ever gets shorter. If it does, it never stays that way for long. In my former tech job, I had a Calvin & Hobbes cartoon of Calvin saying, "God put me on this earth to accomplish a certain number of things. Right now, I am so far behind that I will never die!" Hang in there, Kathleen Principal Lab Technician Dept of Pharmacology & Toxicology Rutgers University Dawn Cowie wrote: >Laura, > Thank you so much for the detailed info. I will keep this in mind if we decide to try microwaving again (we probably will, its just a question of when). If I'm ever up in that area I'll contact you and see if we can arrange a visit. I understand you being busy, its the same here. There never seems to be enough hours in the day to get everything you need done. My list of "to do" things doesn't seem to get shorter. > > Dawn > >"Galiotto, Laura" wrote: > Hello Dawn, > This microwave system can process tissue as thick as 5 mm , does not restrict reagent use (buying reagents from them only leads to increase in cost), it is versatile and has many other programs such as rapid decal, gross hardening and lymph node detection using saline, and gives the freedom of modifying programs. I purchase the same reagents I have always used. I continue to fix in 10%NBF, this is a requirement for FDA approved testing. I have modified the programs to meet our needs, this includes ambient fixation in formalin prior to rapid fixation allowing us to put fresh tissue on it. I am getting a second unit so I may stagger start times and use the other options to help create a safer lab and improve time and quality on case. My next phase is to process breast cores that are thicker than 3mm and bone marrow core biopsies same day. > When I was in the market to purchase our first unit, I discovered this system had more flexibility than other units, others had restricts to size, where we purchase the reagent from and they did not have the versatile use such as rapid decal. > We are located in the northwest suburbs of Chicago, near the Arlington Horse Race Track. If you give me a couple days notice I will make sure my schedule is clear. I spend a lot of time in meetings. > > Laura Galiotto, PBT,HT(ASCP) > Histology Manager > Northwest Community Hospital > Arlington Heights, Il 60056 > 847-618-6190 > -----Original Message----- >From: Dawn Cowie [mailto:dlcowie@prodigy.net] >Sent: Saturday, March 31, 2007 5:27 AM >To: Galiotto, Laura; histonet >Subject: RE: [Histonet] delivery time of last tray of H&E > > > Hello Laura, > Thanks for the info on the RHS. What do you mean by "limitless system"? > Where is your lab located? I would like to see another lab running this microwave. > Thanks, > Dawn > >"Galiotto, Laura" wrote: > Hello Dawn, > We have the RHS-1 and are getting a second one. I like the limitless system. We are processing tissue as thick as 3mm starting the run at 10:30 and handing in the slides at 16:00. It also does not restrict you to a different reagent or vendor for reagents. I continue to use 10%NBF for fixation and ethanol & Isopropanol for dehydration, then immediately into paraffin. > If you are ever in town stop by to see it. > Thanks > Laura > -----Original Message----- >From: Dawn Cowie [mailto:dlcowie@prodigy.net] >Sent: Friday, March 23, 2007 10:11 AM >To: Galiotto, Laura >Subject: RE: [Histonet] delivery time of last tray of H&E > > > Laura, > It is definitely hard to find certified techs willing to do those hours. I'm lucky at the moment - the techs that work these hours want to. I'm not sure what we'll do when 1 of them retires probably in the next couple of years. > What kind of microwave processor do you run? We are going to look at this again. We tried the RHS-1 a few years ago- docs didn't like results - too labour intensive for the techs. > thanks for your response, > > Dawn > >"Galiotto, Laura" wrote: > Hello Dawn, > Yeah, however you do bring up a very valid point. I do get the specialist, ie.. oncologist,pulmonaryologist, that come in very early to check slides before the pathologist gets in. My biggest problem is finding certified technicians that will work the night shift, practically non-existant. > Thank goodness for our microwave processor. We now are processing most biopsies sameday. > Laura Galiotto, HT(ASCP) > Histology Manager > > > >--------------------------------- > From: Dawn Cowie [mailto:dlcowie@prodigy.net] >Sent: Fri 3/23/2007 3:03 AM >To: Douglas D Deltour; 'Marshall Terry Dr, Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; Galiotto, Laura; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] delivery time of last tray of H&E > > > We do what we are required to do by the pathologists. I don't know why they think they need all H&E's so early - they can't look at them all at the same time and sign them out - except that they have them just in case a clinician calls. The trend these days is certainly toward faster and faster turnaround time. > > Dawn L. Cowie, HT (ASCP) > Histology Supervisor > Pensacola Pathologists, PA > Pensacola, Florida 32503 > 850-416-7251 > >Douglas D Deltour wrote: > I don't get it either. We have a little histo pixies that comes in while we >sleep and the slides magically appear on the pathologist desk in the >morning. :) > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall >Terry Dr,Consultant Histopathologist >Sent: Thursday, March 22, 2007 12:28 PM >To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] delivery time of last tray of H&E > >This is all bloody unbelievable. Don't any of you sleep? >What is the point of working at night? > >Terry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi >Allison-Tacha >Sent: 22 March 2007 17:25 >To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] delivery time of last tray of H&E > >Ren?--That was similar to the protocol I followed in the old days before >24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, >provide special stains and recuts and IHC's by 2-3 PM, depending if it >wasn't a lengthy procedure. > >If you have off site facilities to provide for, that is a different >situation. You have to work around courier sheduals for delivering to off >site facilities. Everyone needs their slides and time is money!! > >With the new microwave processing technology that is now in use, there has >been a shift to having the majority of staff starting at 10 PM at night. >MUCH TO THE DISLIKING OF HISTOTECH"S. > >The day shift is minimum. IHC is done during the day shift, after the >pathologist has determined to do so. >In most situations, a battery of IHC's tests are pre-set, as well as with >special stains. > >Akemi Allison-Tacha BS, HT (ASCP) HTL >President >Phoenix Lab Consulting & Staffing >Specializing in Histology, SS, IHC, & Microarray Madison, WI >Tele: (925) 788-0900 >E-Mail: akemiat3377@yahoo.com > >--- Rene J Buesa wrote: > > > >>Laura: >>For a laboratory I used to manage with quite similar annual workload >>we had the following >>schedule: >>1- last tissue accepted at 7:00 PM; >>2- two VIPs atarting with delay to be finished at 4:00 AM next day; >>3- Rushes ready for the pathologists at 8:00 AM next day; >>4-All slides ready, the latest, at 1:00 PM. >>5- All IHC ready by 2 PM >>6- all HC ready by noon. >>Ren? J. >> >>"Galiotto, Laura" wrote: >>Hello Fellow Histo Tech's, >>Will anyone be willing to share the delivery time of the last tray of >>H&E for the days workload to the pathologist reading them? >>What is your cutoff time for specimen acceptance? >>What is your annual case workload? >>I am trying to improve the TAT from specimen gross to the H&E >>delivered to the pathologist. Unless we change the cutoff time I can >>not see how I can do this? Any suggestions? >>We process 22,000 cases a year an average of 280 blks a day consisting >>of types of tissue. >>Please Help >>Laura >> >> >>******************* PLEASE NOTE ******************* This >>E-Mail/telefax message and any documents accompanying this >>transmission may contain information that is privileged, confidential, >>and/or exempt from disclosure under applicable law and is intended >>solely for the addressee(s) named above. If you are not the intended >>addressee/recipient, you are hereby notified that any use of, >>disclosure, copying, distribution, or reliance on the contents of this >>E-Mail/telefax information is strictly prohibited and may result in >>legal action against you. Please reply to the sender advising of the >>error in transmission and immediately delete/destroy the message and >>any accompanying documents. Thank you. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >> >> >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> >> >> >>--------------------------------- >>Sucker-punch spam with award-winning protection. >>Try the free Yahoo! Mail Beta. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >> >> >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ******************* PLEASE NOTE ******************* >This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. > > > ******************* PLEASE NOTE ******************* >This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. > > > ******************* PLEASE NOTE ******************* >This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gcallis <@t> montana.edu Wed Apr 4 09:48:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Apr 4 09:48:48 2007 Subject: [Histonet] Sakura embedding station TEC 5 In-Reply-To: References: <46126E59020000EE00012E44@smtp-gw.hurleymc.com> Message-ID: <6.0.0.22.1.20070404084104.01b64e30@gemini.msu.montana.edu> Along with 2 module design, it also ALL flat surfaces at the embedding/cooling areas and NO handle on lid over paraffin reservoir (we dislike raised handles). This then gives us a handy low temperature heating area if needed. We are extremely happy with this embedding center. At 04:28 AM 4/4/2007, you wrote: >The Sakura also has a 2-module design. >Jeanine Bartlett >Infectious Disease Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From LewisS <@t> pediatrics.ohio-state.edu Wed Apr 4 10:17:56 2007 From: LewisS <@t> pediatrics.ohio-state.edu (Lewis, Sarah) Date: Wed Apr 4 10:18:20 2007 Subject: [Histonet] FW: Muscle lab positions available Message-ID: <714B9F12B4E18C4C843B66E8E190F2AD01340B65@res2k3ms01.CRII.ORG> ________________________________ We currently have a position available at Columbus Childrens Hospital in the Neuromuscular Lab. We specialize in muscle and nerve processing. The person we are looking for will be assisting in the lab for clinical histology needs (Frozen sections, Immunohistochemistry, Enzyme histochemistry and some basic histology). This person will also be responsible for specialized assays specific for muscle proteins (Western blots) . This position will come with extensive training, and requires someone with a scientific background. We also have an Research assistant/animal technician position available. The person will assist in multiple research projects, involving gene therapy applications. The person will be responsible for running basic and molecular biology experiments. They will also assist with research animal surgeries and housing. We have a wonderful working environment here, with lots of exciting clinical diagnostics and research going on. If you are interested in either of these positions please contact Chris Shilling at ShillinC@ccri.net . Sarah E. Lewis Histotechnician Childrens Research Center for Gene Therapy 700 Childrens Dr Rm WA3112 Columbus OH 43205 (614)-722-2204 LewisS@ccri.net From rsrichmond <@t> aol.com Wed Apr 4 10:39:51 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Apr 4 10:40:08 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 5 In-Reply-To: <200704041040.6dc4613b8d78a@rly-mc06.mail.aol.com> References: <200704041040.6dc4613b8d78a@rly-mc06.mail.aol.com> Message-ID: <8C944E69E8C9638-AD4-D83@WEBMAIL-RB08.sysops.aol.com> Stacy McLaughlin asks: >>I'd like to know how many of you have your pathology reports bound into "book form". Is there a requirement to do this?<< Everybody used to do this, but in locum tenens coverage of numerous practices, I don't think I've seen it done in the last 20 years. Bookbinding has become extremely expensive. I have never heard of a regulatory requirement for it, past or present, though the paper-pushers are ever searching for new ways to waste other people's time and money. I still commonly see looseleaf notebooks, file cabinets, and file folders holding reports for many years back. Now that hospitals are going to scanning of reports with storage on disks or other electronic media, I'd think that was the way to go with old reports, though the time you'd need to scan in 10 or 20 years' reports would be non-trivial. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From algranth <@t> u.arizona.edu Wed Apr 4 11:50:10 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Apr 4 11:50:23 2007 Subject: [Histonet] Sakura embedding station TEC 5 In-Reply-To: <6.0.0.22.1.20070404084104.01b64e30@gemini.msu.montana.edu> References: <46126E59020000EE00012E44@smtp-gw.hurleymc.com> Message-ID: <4.3.2.7.2.20070404092208.00d920a8@algranth.inbox.email.arizona.edu> We got the TEC 5 last summer and well, I do like it but I'm not in love with it. I think it is because of my type of lab which is a research histology core facility. We do all kinds of crazy things here - like trimming the samples at embedding for instance. Plus, it is too deep to go where we had our old embedder so we had to put it in anew spot and it is not the most convenient because it is in the flow of traffic and the air vents. We deflected the air but the traffic - no way to do that but to move to a new lab! HA! I don't like that the light is wimpy. Well it is in my estimation. Too dim for my old eyes and it doesn't cover enough area, so I have to have another lamp set up over the embedding area to illuminate the samples for proper orientation. Then, the surface of the embedding area is silver - it reflects light and the glare is something awful. After a long embedding session you just need to go to a dark corner and rest. Because we don't embed every day we never use the auto turn on/off functions. That feature is wasted on us here but I agree it would be great in a clinical lab. We often trim the tissues just before embedding - often we get rodent stomachs and intestines on a membrane to keep them flat and they are usually too tall high for the normal embedding mold so we bisect them lengthwise and embed both halves or we get esophagus with stomach attached and we cut to show the junction, etc. We have rigged up a plastic cover to fit over the stage as a little cutting board as to not scratch the stage but it warps so I am currently looking for another type of material that can get hot, be used as a cutting board and not warp. We used to use a large glass slide on our old embedding center but it moves around too much on this one. However as Gayle pointed out the flat surface on the top of the paraffin reservoir is a wonderful place to air dry slides when the protocol does not allow for over drying. It is nice and warm - not hot - and the slides dry nicely. Andi Grantham At 08:48 AM 4/4/2007 -0600, Gayle Callis wrote: >Along with 2 module design, it also ALL flat surfaces at the >embedding/cooling areas and NO handle on lid over paraffin reservoir (we >dislike raised handles). This then gives us a handy low temperature >heating area if needed. We are extremely happy with this embedding center. > >At 04:28 AM 4/4/2007, you wrote: >>The Sakura also has a 2-module design. >>Jeanine Bartlett >>Infectious Disease Pathology Branch >>(404) 639-3590 >>jeanine.bartlett@cdc.hhs.gov > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From jacobc <@t> mmc.org Wed Apr 4 11:54:48 2007 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Wed Apr 4 11:55:11 2007 Subject: [Histonet] Microwave Processing and IHC's Message-ID: We are currently using the Ventana Benchmark for IHC staining and are thinking about bringing microwave processing into our lab. Does anyone out there know of documentation concerning IHC staining on microwave rpocessed tissue? Is anyone successfully staining IHC's on microwave processed tissue? Did you have to re-validate your antibodies or simply re-test your protocols on microwave processed tissue? Any help with this issue would be greatly appreciated. Chris Jacobs, HT(ASCP), QIHC Histology Dept. NorDx Labs Scarborough, ME jacobc@mmc.org From pmcardle <@t> ebsciences.com Wed Apr 4 12:08:05 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed Apr 4 12:08:15 2007 Subject: [Histonet] Microwave Processing and IHC's In-Reply-To: References: Message-ID: <4613DB75.3010801@ebsciences.com> Hello: If you trust a vendor of laboratory microwaves :-) , I can supply some references. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Christine Jacobs wrote: > We are currently using the Ventana Benchmark for IHC staining and are > thinking about bringing microwave processing into our lab. Does anyone > out there know of documentation concerning IHC staining on microwave > rpocessed tissue? Is anyone successfully staining IHC's on microwave > processed tissue? Did you have to re-validate your antibodies or simply > re-test your protocols on microwave processed tissue? > Any help with this issue would be greatly appreciated. > > Chris Jacobs, HT(ASCP), QIHC > Histology Dept. > NorDx Labs > Scarborough, ME > jacobc@mmc.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Charlene.Henry <@t> STJUDE.ORG Wed Apr 4 12:34:28 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Apr 4 12:34:38 2007 Subject: [Histonet] Microwave Processing and IHC's. . In-Reply-To: Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A20A2@SJMEMXMB02.stjude.sjcrh.local> Chris, We also use the Benchmark XT and we routinely microwave process all of our biopsy tissues. When we purchased our microwave processor, I ran a large range of antibodies comparing routine processing vs microwave processing (same tissues but processed differently) and found that the all of the IHC tests performed on the microwave tissues were equal to or better than the tissues routinely processed. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Jacobs Sent: Wednesday, April 04, 2007 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing and IHC's. . We are currently using the Ventana Benchmark for IHC staining and are thinking about bringing microwave processing into our lab. Does anyone out there know of documentation concerning IHC staining on microwave rpocessed tissue? Is anyone successfully staining IHC's on microwave processed tissue? Did you have to re-validate your antibodies or simply re-test your protocols on microwave processed tissue? Any help with this issue would be greatly appreciated. Chris Jacobs, HT(ASCP), QIHC Histology Dept. NorDx Labs Scarborough, ME jacobc@mmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed Apr 4 12:55:11 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Apr 4 12:55:25 2007 Subject: [Histonet] BUDGET ITEM - TEM Message-ID: We currently have an H-600 Transmission Electron Microscope that still works however we are looking to see what is available new. I have contacted Hitachi and know about their new model. Does anybody have any suggestions about diagnostic transmission microscopes available on the market? We primarily use our instrument for kidney biopsies, muscle biopsies, and nerve biopsies. Obviously the use of EM over the last twenty five years has changed and high magnification transmission EM is used less and less. We are interested in a small diagnostic TEM with a digital camera system. Thanks. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Shirley_PHUA <@t> hsa.gov.sg Wed Apr 4 13:07:03 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed Apr 4 13:09:25 2007 Subject: [Histonet] Shirley is away 04 April 2007's afternoon ... Message-ID: I will be out of the office from 04-04-2007 to 05-04-2007. I will return on 05 April 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Apr 4 14:12:32 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Apr 4 14:16:49 2007 Subject: [Histonet] Tennessee Society for Histotechnology Meeting Information Message-ID: <898D946569A27444B65667A49C074052D0E19B@mailbe06.mc.vanderbilt.edu> We are pleased to announce that programs have been mailed for our annual meeting. The meeting will be June 7-9 in Dickson, TN. Workshop and Lecture topics include: * IHC- Past, Present and Future: Where we have been and where we are going. *Management in the Laboratory *Rapid Tissue Processing Without Microwave *The Role of Immunohistochemistry in Neuropathology: Brain Tumors to Neurodegenerative Disease *MOHS Micrographic Surgery- What is it? *Grossing Bones (Techniques, Instruments, Decalcification) * The Power of Teamwork *How to Prepare Macro Sections *Basic Immunohistochemistry *Methyl Methacrylate - Why and How? The Keynote address, Friday after lunch will be presented by Paul E. Billings, Jr. and is entitled "Whale Done: The power of Positive Relationships in Working Together". The address is included in the price of registration and is followed by a Cybersphere Laser Light Show. Our main entertainment this year will be the dinner Theatre production of "Chicago" on Friday evening. For More information or to request an official program, please contact me "off list". We are also still accepting exhibiting companies. Please contact me for this also. We hope to see you in June! Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 phone (615) 343-7089 fax TSH President NSH Quality Control Committee Chair From Susan.Wert <@t> cchmc.org Wed Apr 4 14:29:48 2007 From: Susan.Wert <@t> cchmc.org (Susan Wert) Date: Wed Apr 4 14:30:26 2007 Subject: [Histonet] Re: frozen sections falling off slides Message-ID: Response to problem with frozen sections falling off slides. We have long worked with frozen sections for IHC and ISH (with up to 21 washes!) without losing sections. We use polysine coated slides not superfrost or charged slides. Let sections dry on the slides at room temperature overnight and then fix with 2-4% PFA for 5-10 minutes. Lay slides flat on absorbent paper in the hood and flood with the fixative. Rinse in PPBS. For more difficult tissues, we also use silanated slides or silylated slides. Again dry overnight at room temperature and fix with 2-4% PFA for 5-10 minutes. The alehydes in the tissue will thn form covalent bonds with the silane coating on the slide. Fixation is required and sections must be dry. Cordially, Susan E. Wert, Ph.D. Associate Professor of Pediatrics Director, Molecular Morphology Core Division of Pulmonary Biology, ML 7029 Cincinnati Children's Hospital Medical Center 3333 Burnet Avenue Cincinnati, Ohio 45229-3039 TEL: 513-636-4297 (office, voice mail) 513-636-3522 (laboratory) FAX: 513-636-7868 E-mail: Susan.Wert@CCHMC.org This e-mail message (including attached files) contains information that may be privileged and confidential. It is intended only for the individual or entity to which it is addressed. Any other distribution copying or disclosure is strictly prohibited. If you have received this message in error, please delete it or notify the sender by telephone or by return e-mail. >>> histonet-request@lists.utsouthwestern.edu 04/04/07 10:44 AM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Sakura embedding station TEC 5 (Dawn Cowie) 2. double-staining with two rabbit antibodies, Zenon-kit (Yves Heremans) 3. Binding pathology reports (Stacy McLaughlin) 4. RE: Sakura embedding station TEC 5 (Trajkovic, Dusko) 5. negatives (Renko, Heather D.) 6. double-staining with two rabbit antibodies, Zenon-kit (AGrobe2555@aol.com) 7. RE: Sakura embedding station TEC 5 (Lynette Pavelich) 8. Grosslab operation instructions (Stacy McLaughlin) 9. Binding Pathology reports-THANK YOU!! (Stacy McLaughlin) 10. AW: [Histonet] negatives (Gudrun Lang) 11. RE: negatives (Cazares, Ruth) 12. frozen sections falling off slides (Gayle Callis) 13. RE: Sakura embedding station TEC 5 (Alan Bishop) 14. RE: Sakura embedding station TEC 5 (Dawn Cowie) 15. RE: Sakura embedding station TEC 5 (Bartlett, Jeanine (CDC/CCID/NCZVED)) 16. Maryland Histo/Cyto positions (Michael LaFriniere) 17. CD61 (Mildred Fail) 18. RE: CD61 (Sebree Linda A.) 19. Missouri Society for Histotechnology - Annual Meeting - May 31, June 1-2, 2007 - The Lodge of the Ozarks, Branson, Missouri (Johnson, Teri) 20. RE: delivery time of last tray of H&E (Dawn Cowie) 21. Re: delivery time of last tray of H&E (Kathleen Roberts) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Apr 2007 11:05:02 -0700 (PDT) From: Dawn Cowie Subject: Re: [Histonet] Sakura embedding station TEC 5 To: Amy Lee , histonet Message-ID: <382174.76486.qm@web81007.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amy, The embedding station I like to work with the best is the Leica EG1160. We purchased a Sakura TEC5. The one quirk we noticed is that you cannot program it to come on before midnight and go off the next morning. For example, we start embedding at 10:30pm and wanted it to go off at 9am the next day. If it's before midnight it won't let you program it this way. Sakura doesn't have an answer for this. Otherwise, its a good instrument. Dawn Amy Lee wrote: Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 3 Apr 2007 20:11:51 +0200 (CEST) From: Yves Heremans Subject: [Histonet] double-staining with two rabbit antibodies, Zenon-kit To: histonet@lists.utsouthwestern.edu Message-ID: <20070403181151.020EA532@bonito.ulb.ac.be> Dear All, We need to double-stain with two antibodies made in rabbit and would prefer to use fluorescent detection for both. I know that Molecular Probes has Zenon kits for differential fluorescent labeling of rabbit antibodies, allowing you to use multiple antibodies from the same species, however these kits require that you know the concentration of the antibodies to be labeled. Since our rabbit antibodies are not affinity-purified but consist of whole serum, is there a way around this and use the Zenon kit anyway ? Yves ------------------------------ Message: 3 Date: Tue, 3 Apr 2007 14:38:08 -0400 From: "Stacy McLaughlin" Subject: [Histonet] Binding pathology reports To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I'd like to know how many of you have your pathology reports bound into "book form". Is there a requirement to do this? My reason for asking- our pathology secretary insists this needs to be done, she says all hospitals she knows do this. It is taking up lots of storage space. All of the reports are in our computer system. Thanks for your help! Stacy McLaughlin ------------------------------ Message: 4 Date: Tue, 3 Apr 2007 11:44:52 -0700 From: "Trajkovic, Dusko" Subject: RE: [Histonet] Sakura embedding station TEC 5 To: "Amy Lee" , "histonet" Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B20431151D@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="us-ascii" We have a Sekura TEC 5 and a Leica. Everyone (4 colleagues) prefers the Sekura. On Sakura, paraffin, cassette, and embedding surface are on all of the time. Whenever we need to embed, the cryo chamber is turned on and once the work is done, cryo is turned off. Try doing that with a Leica. Either everything is on, or everything is off. Smart engineering. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, April 03, 2007 9:35 AM To: histonet Subject: [Histonet] Sakura embedding station TEC 5 Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. ------------------------------ Message: 5 Date: Tue, 3 Apr 2007 14:04:01 -0500 From: "Renko, Heather D." Subject: [Histonet] negatives To: histonet@lists.utsouthwestern.edu Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612CCA@pmc-rfd-mx01.intranet.osfnet.org> Content-Type: text/plain; charset=iso-8859-1 Histo netters: I got a call from a fellow tech asking me if I run a negative control on my special stains and my H&E slides. I was completely stumped because I have always used my internal negative and an a positive for each stain. My explanation is that the positive is for the test and the negative is for the patient. I have only run negative for higher testing such as IHC, ISH, etc. Can someone help me to help my fellow tech with the explanation. Nancy washkowiak, Ottawa Community Hospital 'nwash_24@yahoo.com' Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== ------------------------------ Message: 6 Date: Tue, 3 Apr 2007 15:05:18 EDT From: AGrobe2555@aol.com Subject: [Histonet] double-staining with two rabbit antibodies, Zenon-kit To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Yves, Just do the complete stain for one (block, rabbit primary#1, fluorescent secondary#1), block well and then do the second (rabbit primary#2, fluorescent secondary#2). Use two different color anti-rabbit fluorophores. I have done this in the past with no apparent problems. Another option is to pre-react the two primaries with different anti-rabbit fluorophores in separate tubes, and then add to the section. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. ------------------------------ Message: 7 Date: Tue, 03 Apr 2007 15:10:16 -0400 From: "Lynette Pavelich" Subject: RE: [Histonet] Sakura embedding station TEC 5 To: ,, Message-ID: <46126E59020000EE00012E44@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Amy, Our hospital has a Leica EG1040H (hot plate) and a EG1040C (cold plate). These two units are separate. This makes it cheaper to replace one if one or the other 'dies'. You have the option on the heating side to program what time you want it to start heating, what days to heat, or the option to always have it "on". The cold side has a "on" or "off" switch. We like the equipment. They have made some very good changes with the wax collection drawer on the heat side and we are very happy. hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Trajkovic, Dusko" 04/03/07 2:44 PM >>> We have a Sekura TEC 5 and a Leica. Everyone (4 colleagues) prefers the Sekura. On Sakura, paraffin, cassette, and embedding surface are on all of the time. Whenever we need to embed, the cryo chamber is turned on and once the work is done, cryo is turned off. Try doing that with a Leica. Either everything is on, or everything is off. Smart engineering. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, April 03, 2007 9:35 AM To: histonet Subject: [Histonet] Sakura embedding station TEC 5 Hello histonetters, Is anybody using this equipment? How do you like/dislike it? We are looking for a new embedding station. Any input is highly appreciated! Amy --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 3 Apr 2007 15:12:08 -0400 From: "Stacy McLaughlin" Subject: [Histonet] Grosslab operation instructions To: Message-ID: childrensmemorial.org Wed Apr 4 16:20:11 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Apr 4 16:18:56 2007 Subject: [Histonet] (no subject) Message-ID: Dear Histoneters, It is time for me to order more reagents and I need your suggestion. I was using the RA's reagents: Streptavidin HRP, Citrate buffer, TRIS buffer, Ab Diluent, Protein block. I would like to have opinion of histos who use/used RA's reagents. If any of you used it before and did switch to DAKO or another company I would like to here a reason? Hope to here from you as soon as possible! Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From msviapiano <@t> yahoo.com Wed Apr 4 17:18:46 2007 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Wed Apr 4 17:18:51 2007 Subject: [Histonet] CD92 / CDw92 / CTL1 Message-ID: <20070404221846.10282.qmail@web54506.mail.re2.yahoo.com> Hello everyone, Does anybody know about (or have) an anti-CD92 antibody? This protein is also known as choline-transporter-like 1 (CTL1) or solute carrier SLC44A1. The only one I found online is from Abnova but the last two Abs I bought from them were useless for all applications... Thank you! Mariano Mariano S. Viapiano, PhD Assistant Professor Center for Molecular Neurobiology and Department of Neurological Surgery The Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 ____________________________________________________________________________________ The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. http://searchmarketing.yahoo.com/arp/sponsoredsearch_v2.php From jwatson <@t> gnf.org Wed Apr 4 17:18:41 2007 From: jwatson <@t> gnf.org (James Watson) Date: Wed Apr 4 17:18:51 2007 Subject: [Histonet] CSH meeting Message-ID: Has anyone gotten any information on the CSH meeting in May? James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org From rhrogers <@t> iupui.edu Wed Apr 4 17:31:50 2007 From: rhrogers <@t> iupui.edu (Rogers, Rhonda) Date: Wed Apr 4 17:31:54 2007 Subject: [Histonet] Myosin Heavy Chain (slow) Antibodies Message-ID: <0263AA7A7CB6FF42AA4048567A26EBC9458B2A@iu-mssg-mbx107.ads.iu.edu> Hello, I am looking for a myosin heavy chain (slow) antibody that will work on FFPE sections. I will be using mouse embryo sections. If anyone is willing to share recommendations or protocols, I would appreciate some help. Thanks, Rhonda Rhonda Rogers, HTL(ASCP) Wells Center for Pediatric Research - Conway Laboratory Indiana University Purdue University Indianapolis 1044 W. Walnut St., R4 379 Indianapolis, IN 46202-5225 Phone: 317-278-8781 E-mail: rhrogers@iupui.edu From chesarato <@t> hotmail.com Wed Apr 4 17:58:22 2007 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Wed Apr 4 17:58:29 2007 Subject: [Histonet] C4d Clone A 213 Antibody on Paraffin Sections ! Message-ID: Dear People Does anybody know how to use the Monoclonal Antibody C4d Clone A213 from Quidel Corp. in paraffin sections? Thank you in advance. Cesar Romero Buenos Aires Argentina _________________________________________________________________ S? uno de los primeros a testar el Windows Live Messenger beta. [1]?Haz click aqu?! References 1. http://g.msn.com/8HMAESAR/2731??PS=47575 From JMacDonald <@t> mtsac.edu Wed Apr 4 19:10:40 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Apr 4 19:10:44 2007 Subject: [Histonet] CSH meeting In-Reply-To: Message-ID: The CSH Symposium is scheduled for May 17-20 in San Mateo. The programs have not gone out yet. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "James Watson" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/04/2007 03:18 PM To cc Subject [Histonet] CSH meeting Has anyone gotten any information on the CSH meeting in May? James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zumbor <@t> email.cs.nsw.gov.au Wed Apr 4 22:30:06 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Wed Apr 4 22:35:06 2007 Subject: [Histonet] Olympus eyepeice Message-ID: <01C77786.86B1A2E0.zumbor@email.cs.nsw.gov.au> Hi All, I'm looking for an Olympus NFK photo eyepeice 1.67x. Does anyone have a spare one or one they would be willing to sell. Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From Marilyn.Tyler <@t> uct.ac.za Thu Apr 5 05:37:32 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Thu Apr 5 05:37:56 2007 Subject: [Histonet] mouse pathology Message-ID: <4614ED8B.A78E.0090.0@uct.ac.za> Hi all Histonetters Please could someone suggest a good book on mouse and human pathology(histology) for research Histologists Thanks Marilyn From settembr <@t> umdnj.edu Thu Apr 5 06:40:08 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu Apr 5 06:41:24 2007 Subject: [Histonet] Myosin Heavy Chain (slow) Antibodies Message-ID: Hello Rhonda, I use NovoCastra's Myosin Heavy Chain (slow) on FFPE human tissue. The spec sheet indicates that this antibody reacts with mouse among others. NovoCastra is distributed in by Vision BioSystems in Norwell, MA 800-753-7264 The cat. # is: NCL-MHCs I use normal muscle as positive control. I pretreat with Dako's Target Retrieval Sol'n (TRS) in a steamer for 40 min. (but I have pretreated other ways and they work too) I use it at a 1:40 dilution. It looks good (on human tissue) Good Luck Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Rogers, Rhonda" 04/04/07 6:31 PM >>> Hello, I am looking for a myosin heavy chain (slow) antibody that will work on FFPE sections. I will be using mouse embryo sections. If anyone is willing to share recommendations or protocols, I would appreciate some help. Thanks, Rhonda Rhonda Rogers, HTL(ASCP) Wells Center for Pediatric Research - Conway Laboratory Indiana University Purdue University Indianapolis 1044 W. Walnut St., R4 379 Indianapolis, IN 46202-5225 Phone: 317-278-8781 E-mail: rhrogers@iupui.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Thu Apr 5 08:12:19 2007 From: TillRenee <@t> uams.edu (Till, Renee) Date: Thu Apr 5 08:12:42 2007 Subject: [Histonet] positive negative Message-ID: <11F927674DEBDC43B960809A7403C5D203F110C6@MAILPED.ad.uams.edu> Why would a negative control have more and darker staining than the actual test slides with the antibody? I am used to adjusting dilutions when both are too high, but what to do when lowering a dilution to get rid of background, gets rid of what little staining you have? I am using avidin/biotin blocks in addition to the normal serum block. Could it be something to do with the tissue? I am having this problem with rat liver, though I never have it with other rat tissues. It is a PCNA stain I am already running it at 1:10,000, which is way less than what I run it on any other tissue. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From SDrew <@t> uwhealth.org Thu Apr 5 08:35:24 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Thu Apr 5 08:35:35 2007 Subject: [Histonet] C4d Clone A 213 Antibody on Paraffin Sections ! In-Reply-To: Message-ID: I was unimpresssed with the results I got trying Quidel's antibody on paraffin tissue (we use Ventana instrumentation). I would actually recommend using the polyclonal C4d from American Research Products for paraffin tissues if you have the option to switch. Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cesar Francisco Romero Sent: Wednesday, April 04, 2007 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d Clone A 213 Antibody on Paraffin Sections ! Dear People Does anybody know how to use the Monoclonal Antibody C4d Clone A213 from Quidel Corp. in paraffin sections? Thank you in advance. Cesar Romero Buenos Aires Argentina _________________________________________________________________ S? uno de los primeros a testar el Windows Live Messenger beta. [1]?Haz click aqu?! References 1. http://g.msn.com/8HMAESAR/2731??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lao_ji <@t> yahoo.com Thu Apr 5 09:37:34 2007 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Thu Apr 5 09:37:44 2007 Subject: [Histonet] double-staining with two rabbit antibodies, Zenon-kit In-Reply-To: Message-ID: <759725.2021.qm@web30711.mail.mud.yahoo.com> Albert, Can you tell me how to do the "block well" before implementing the second (rabbit primary#2) set Ab staining? Thanks in advance! Jimmy --- AGrobe2555@aol.com wrote: > Yves, > Just do the complete stain for one (block, rabbit > primary#1, fluorescent > secondary#1), block well and then do the second > (rabbit primary#2, fluorescent > secondary#2). Use two different color anti-rabbit > fluorophores. I have done > this in the past with no apparent problems. > > Another option is to pre-react the two primaries > with different anti-rabbit > fluorophores in separate tubes, and then add to the > section. > > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > ************************************** See what's > free at http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Get your own web address. Have a HUGE year through Yahoo! Small Business. http://smallbusiness.yahoo.com/domains/?p=BESTDEAL From gcallis <@t> montana.edu Thu Apr 5 09:40:16 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Apr 5 09:40:25 2007 Subject: [Histonet] mouse pathology In-Reply-To: <4614ED8B.A78E.0090.0@uct.ac.za> References: <4614ED8B.A78E.0090.0@uct.ac.za> Message-ID: <6.0.0.22.1.20070405083006.01b45c50@gemini.msu.montana.edu> I presume you are looking for a book showing morphology of both human and mouse, or the study of Histology which is different than Pathology. Wheaters Functional Histology book is excellent for most purposes. Also, the newer edition of Histology, A text and atlas - written by Michael H. Ross. As for pathology, medical libraries and publisher will have many, and pricey books on Human Pathology. As for the mouse, the one book that was superior for normal tissue histology is no longer in print. You may have to do website searches for mouse histology books or even a website devoted to murine, showing the tissue sections. Some Histonetters have addressed the mouse histology text issue in the past. Try the new Histonet Archives search, there are many wonderful links now. Go to this website, http://www.rodentia.com/wmc/ aka Whole Mouse Catalog, I saw books yesterday but did not pay particular attention to the titles nor subject. Good luck At 04:37 AM 4/5/2007, you wrote: >Please could someone suggest a good book on mouse and human >pathology(histology) for research Histologists Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From anh2006 <@t> med.cornell.edu Thu Apr 5 10:02:21 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Apr 5 10:02:42 2007 Subject: [Histonet] mouse pathology In-Reply-To: <6.0.0.22.1.20070405083006.01b45c50@gemini.msu.montana.edu> References: <4614ED8B.A78E.0090.0@uct.ac.za> <6.0.0.22.1.20070405083006.01b45c50@gemini.msu.montana.edu> Message-ID: My favorite human histology book for the lab lately is: Histology: A Text and Atlas With Cell and Molecular Biology Michael H. Ross Gordon I. Kaye Wokciech Pawlina ISBN: 0-683-30242-6 (I am sure there is probably a new edition) It puts histology into a functional context. The detail is quite complete and has been the key to understanding several of our findings in the lab here lately. And a good comprehensive mouse histology text is like the holy grail unfortunately. Luckily there are several specialized histology texts and atlases which do the trick in a pinch like the phenomal Paxinos atlases for mouse brain (adult and during development) and The Atlas of Mouse Development (Kaufmann, ISBN 0-12-402035-6) for embryogenesis. >At 04:37 AM 4/5/2007, you wrote: >>Please could someone suggest a good book on mouse and human >>pathology(histology) for research Histologists -- From bruce.webber <@t> cefe.cnrs.fr Thu Apr 5 10:38:45 2007 From: bruce.webber <@t> cefe.cnrs.fr (Bruce Webber) Date: Thu Apr 5 10:38:57 2007 Subject: [Histonet] In situ hybridization on insects: fixing, processing & cuticle softening advice Message-ID: <46151805.8020602@cefe.cnrs.fr> Hi Histonetters, I am looking for advice on preparing and processing small (c. 2-3mm long) adult ants (i.e. with a well-formed chitinous exoskeleton) for in situ hybridization (ISH) detection of bacteria (using DNA probes to target RNA). The ants are transverse sectioned into 3 parts before fixing to allow greater penetration into each of the 3 main body cavities. I've trawled the archives for various options and have consulted my histological mentor, Bruce Abaloz, but after not finding what I was looking for, I would greatly appreciate advice, opinions and recommendations from others. I do promise to post a summary of any responses together with any methodological tips I found along the way! The main problem I see is dealing with the cuticle to allow for good sectioning (5-8um), but using chemicals & methods that don't interfere with ISH, damage the target RNA, or interfere with the efficiency of the ISH probes. My understanding is that the following factors need to be addressed: 1) Fixing the tissue: The only available material available at this stage was fixed in 2% gluteraldehyde (12h at 4?C) and then stored (> 3 weeks) in 80% EtOH (meaning that gut excision is not an option due to brittle material). In the future, 4% paraformaldehyde, 10% neutral buffered formalin or just straight into 70% EtOH (with slightly shorter storage times) could also be considered as options if people feel that they may be better (the latter option would certainly increase the availability of material). 2) Softening the cuticle: IMHO this is the biggest potential area for chemical damage/interference with ISH. Proposals I am aware of that would not be compatible with ISH due to acidic damage of the RNA are [1] Perenyi's fluid (4:3:3, 10% nitric acid:100% EtOH:0.05% chromic acid); [2] Diaphanol (50% glacial acetic acid saturated with ClO2); and [3] Bouin's fixative (3:1:2, saturated picric acid:formaldehyde:glacial acetic acid). However, other suggestions from previous posts which I know very little about include [4] chloral hydrate processing (after tissue dehydration, melt equal parts of chloral hydrate & phenol and immerse tissue for up to 7 days, followed by chloroform as an intermediary agent); [5] Mollifex Gurr (glycerol, phenol, acetone and EtOH solution, applied to the cutting surface of the paraffin block); [6] Nair (as in the hair removal cream - thioglycolate salts & calcium hydroxide, between fixing and processing, soak the tissue for c. 24hrs); and [7] Phenol (after fixation, soak fixed specimens in 4% phenol (in 80% EtOH) for 24 hours). Lastly, there is 1% DMSO (added to the fixative), but given that the ant is chopped into 3 sections, this may not be necessary to ensure adequate penetration (but does DMSO also improve sectioning?). 3) Processing the tissue: The ongoing debate of xylene v histoclear (or histosolve)..... Histoclear is probably safer, but does xylene produce better results? Has anyone had experience with either on similar invertebrate material? Others have suggested using isopropyl alcohol (instead of EtOH) for dehydration as is tends to have a less hardening effect on the tissue. 4) Material for embedding: I've seen it argued that tougher material such as that containing chitinous exoskeletons benefits from using a harder embedding medium (e.g. TissuePrep 2 from Fisher) or one with better infiltration (e.g. Paraplast X-tra) over standard paraffin. I've also read that Paraplast Plus (containing DMSO) makes sectioning of cuticles easier. Any opinions/recommendations/experience with similar material and any of these embedding mediums would be appreciated! Thank you all in advance for your help with this matter (I apologise for the length of this post!). Bruce Webber Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, France (Key words: cuticle, exoskeleton, chitin, insect, invertebrate, ant, in situ hybridization, ISH, softening.) From brett_connolly <@t> merck.com Thu Apr 5 11:45:32 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Apr 5 11:45:57 2007 Subject: [Histonet] anti-Ornithine Decarboxylase Message-ID: <355C35514FEAC9458F75947F5270974D01896294@usctmx1103.merck.com> Hi all, Looking for everyone's favorite anti-ODC antibody for use on FFPE tissues. Any recommendations? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From mcauliff <@t> umdnj.edu Thu Apr 5 11:49:55 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 5 11:49:59 2007 Subject: [Histonet] mouse pathology In-Reply-To: References: <4614ED8B.A78E.0090.0@uct.ac.za> <6.0.0.22.1.20070405083006.01b45c50@gemini.msu.montana.edu> Message-ID: <461528B3.7080201@umdnj.edu> Dear List: There are lots of good histology books out there, almost any edition of Wheater is good, as are Color Textbook of Histology by Gartner and Hiatt or Basic Histology by Junqueira and Carnerio. The atlas by Gartner and Hiatt is also very good. I am very surprised to see anyone recommend the Ross, Kaye and Pawlina book (see Andrea Hooper's reply below). I reviewed this book for the publisher and I found it to be noticably inferior to the earlier editons by Ross and Romrell. Geoff Andrea Hooper wrote: > My favorite human histology book for the lab lately is: > > Histology: A Text and Atlas > With Cell and Molecular Biology > Michael H. Ross > Gordon I. Kaye > Wokciech Pawlina > ISBN: 0-683-30242-6 (I am sure there is probably a new edition) > > It puts histology into a functional context. The detail is quite > complete and has been the key to understanding several of our findings > in the lab here lately. > > And a good comprehensive mouse histology text is like the holy grail > unfortunately. Luckily there are several specialized histology texts > and atlases which do the trick in a pinch like the phenomal Paxinos > atlases for mouse brain (adult and during development) and The Atlas > of Mouse Development (Kaufmann, ISBN 0-12-402035-6) for embryogenesis. > > > >> At 04:37 AM 4/5/2007, you wrote: >> >>> Please could someone suggest a good book on mouse and human >>> pathology(histology) for research Histologists >> > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From kimtournear <@t> yahoo.com Thu Apr 5 11:54:05 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Apr 5 11:54:15 2007 Subject: [Histonet] what's wrong with the histonet????? Message-ID: <886476.27093.qm@web37713.mail.mud.yahoo.com> Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Tucson, AZ ~~ Don't be afraid your life will end, be afraid it will never begin ~~ --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From Jason.Wiese <@t> va.gov Thu Apr 5 11:58:37 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Thu Apr 5 11:58:46 2007 Subject: [Histonet] PD's for PA's Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> Hello All! I hope this message finds you all healthy and happy... I am hoping someone in histoland can help me. I need to come up with some position descriptions for Pathologist Assistants. I would really like to find a PA that works for the Department of Veteran Affairs, but anything will help. I need to know the series and grade for a PA in the VA system. Thanks you in advance! Jason Jason E. Wiese, BS, HT(ASCP) VAROS Histology/Cytology 913 NW Garden Valley Blvd. Roseburg, OR 97470 (541)440-1000 ext. 44751 jason.wiese@med.va.gov From carl.hobbs <@t> kcl.ac.uk Thu Apr 5 13:54:24 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Apr 5 13:55:03 2007 Subject: [Histonet] Re: Slow Myosin heavy chain Abs Message-ID: <001701c777b3$d4666480$4101a8c0@carlba65530bda> Be careful with mouse embryonic tissues: they do not neccessarily express the classical adult profile of immunostaining, imho. The Novacastra Ab is great. I just happen to use Chemicon's mab1628 which is equally good for pwax mouse sections, optimally after HIER. I assume that you will be using adult mouse muscle to confirm the fidelity of your embryonic positivity? If yes, you must, again imho, also use an anti fast MHC Ab: Sigma's MY32 is very good for me. Demonstrate that anti MHC fast/slow Abs work as expected on a particular adult mouse muscle ( one that has a mixed population of fast/slow fibres) then use anti fast AND slow MHC on your embyronic sections on the same muscle, as your control. Also, imho, you must use an anti Desmin Ab, for the record. Just my opinion........ Please come back with your thoughts..and then results! carl From kimtournear <@t> yahoo.com Thu Apr 5 14:39:03 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Apr 5 14:39:13 2007 Subject: [Histonet] RE: what's wrong with the histonet Message-ID: <227938.69764.qm@web37709.mail.mud.yahoo.com> My fault...our server was being worked on....duh! blonde moment...... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Tucson, AZ ~~ Don't be afraid your life will end, be afraid it will never begin ~~ --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From Tracey.Lenek <@t> CLS.ab.ca Thu Apr 5 16:09:11 2007 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Thu Apr 5 16:10:34 2007 Subject: [Histonet] Somatostatin Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE040BB8F4@mail1.calgary.com> Hi, Does anyone know of a lab performing IHC for Somatostatin on all 5 of its sub-types? We have a pathologist in our lab interested in sending out a case for staining on these antibodies. Any help would be appreciated. Thanks Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From shiy <@t> uthscsa.edu Thu Apr 5 16:53:34 2007 From: shiy <@t> uthscsa.edu (Yun Shi) Date: Thu Apr 5 16:53:50 2007 Subject: [Histonet] Myosin Heavy Chain (slow) Antibodies In-Reply-To: <0263AA7A7CB6FF42AA4048567A26EBC9458B2A@iu-mssg-mbx107.ads.iu.edu> References: <0263AA7A7CB6FF42AA4048567A26EBC9458B2A@iu-mssg-mbx107.ads.iu.edu> Message-ID: <46156FDE.8030800@uthscsa.edu> According to Sigma's certificate I got for monoclonal anti-skeletal myosin (slow) antibody clone NOQ7.5.4D (Product # M8421) , it should work on (protease digested) FFPE tissue sections. But it does say in the product info that detection in FFPE tissues is greatly enhanced by proteolytic digestion of the preparation. Hope this helps. Yun Physiology University of Texas Health Science Center at San Antonio 210-562-6117 Rogers, Rhonda wrote: > Hello, > > > > I am looking for a myosin heavy chain (slow) antibody that will work on > FFPE sections. I will be using mouse embryo sections. If anyone is > willing to share recommendations or protocols, I would appreciate some > help. > > > > Thanks, > > > > Rhonda > > > > Rhonda Rogers, HTL(ASCP) > > Wells Center for Pediatric Research - Conway Laboratory > > Indiana University Purdue University Indianapolis > > 1044 W. Walnut St., R4 379 > > Indianapolis, IN 46202-5225 > > > > Phone: 317-278-8781 E-mail: rhrogers@iupui.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From barbara.bublava <@t> meduniwien.ac.at Fri Apr 6 00:53:58 2007 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Apr 6 00:51:31 2007 Subject: [Histonet] why do we use xylene? was - Peloris Processor and isopropanol In-Reply-To: <000901c775d5$109f2ee0$6412a8c0@dielangs.at> References: <000901c775d5$109f2ee0$6412a8c0@dielangs.at> Message-ID: <4615E076.3010406@meduniwien.ac.at> Dear Histonetters, Hallo Gudrun, In 1968 Romeis (german book about Microscopic Techiniques) named Isopropanol as an Intermedium when used at 56?C. In a later edition ( I dont have it by hand right now) he once more mentions the potential of Isopropanol. Searching Histonet archives or Google also gives some results One protocol suggested isopropanol with 50?C at the last change and then going directly to paraffin. I did try that and neither me nor my pathologist could see differences to usual xylene processing (on autopsy material; HE, Trichrome; Chloroacetate Esterase, Prussian Blue). For me it sounds too good to be true. I am afraid of missing something important because IF it is that easy - why do we all use hazardous xylene or expensive and smelly substitutes on our processors? Maybe someone out there can tell us why isopropanol isn?t the intermedium of choice? Barbara Bublava Dep. of Forensic Medicine Vienna, Austria Gudrun Lang wrote: > Yes, I've been told, that it works. But what is the histochemical background? > Proper fixed tissue would'nt suffer too much from high temperatures, I think. What is about too thick, underfixed tissue? Wouldn't it become hard and tough, difficult to cut? What about shrinkage? > What about collagen-rich tissue? I don't want to produce glue. > Is the whole process sensitiv to the duration in the high temp.? What is the recommandation for the maximum duration in 80 degrees? > What are the critical points? > > Many questions, I know. But I don't like black boxes. > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: Phil McArdle [mailto:pmcardle@ebsciences.com] > Gesendet: Montag, 02. April 2007 20:30 > An: gu.lang@gmx.at > Cc: histonet@lists.utsouthwestern.edu > Betreff: Re: AW: [Histonet] Peloris Processor and isopropanol > > I'm with a laboratory microwave vendor: > > Never mind 74? C - some microwave protocols call for temperatures as > high as 82? or 84? C (!) in order to exceed the boiling point of > isopropanol, facilitating its replacement with paraffin. Naturally, this > caused some concern and resistance in the anatomic pathology community. > However, look at your own question about IHC: when you consider antigen > retrieval protocols call for 100?C, even 84? pales by comparison. Of > course, there are many reasons you want to minimize the amount of time > spent at high temperatures, but there is no problem with the majority of > tissue types for the relatively short timeframes involved in microwave > processing. > > Also, by the paraffin step, the tissue has already been fixed, > dehydrated, and defatted; while it is in a sense still "tissue," it's > radically different from the tissue as received. You certainly don?t > want to burn or "cook" anything, but 82?C isn?t really extreme in this > context. (I'd be at least as concerned about how a given paraffin and > additives hold up at high temperatures, so it's always a good idea to > check with the manufacturer.) There also is the issue of vacuum; since > the purpose of high temperature is to get above the boiling point of > isopropanol, and vacuum causes BP depression, vacuum should allow you to > lower the temperature to something you're more comfortable with. > > More information may be found at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Very best regards, > > Phil McArdle > From akemiat3377 <@t> yahoo.com Fri Apr 6 09:27:23 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Apr 6 09:27:28 2007 Subject: [Histonet] Is the histonet working? Message-ID: <718644.40975.qm@web31311.mail.mud.yahoo.com> Hi--Happy Good Friday! Hope all of you have a Great Easter Sunday. I haven't got any postings in the last 2 days. That's pretty unusual. Did I accidently unsubscribe? Akemi Allison-Tacha From Jackie.O'Connor <@t> abbott.com Fri Apr 6 09:39:34 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Apr 6 09:40:22 2007 Subject: [Histonet] Is the histonet working? In-Reply-To: <718644.40975.qm@web31311.mail.mud.yahoo.com> Message-ID: We voted you off the island, Akemi. Akemi Allison-Tacha Sent by: histonet-bounces@lists.utsouthwestern.edu 04/06/2007 09:27 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Is the histonet working? Hi--Happy Good Friday! Hope all of you have a Great Easter Sunday. I haven't got any postings in the last 2 days. That's pretty unusual. Did I accidently unsubscribe? Akemi Allison-Tacha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Apr 6 09:49:53 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Apr 6 09:50:01 2007 Subject: [Histonet] Is the histonet working? In-Reply-To: Message-ID: <781810.87691.qm@web31314.mail.mud.yahoo.com> OK-It's definitely Friday and I'll put on my traveling clothes and head home! Only thing is, it's 32 degrees out there! Boy do I miss that California weather!! Can I have a second chance please? Akemi --- Jackie M O'Connor wrote: > We voted you off the island, Akemi. > > > > > > Akemi Allison-Tacha > Sent by: histonet-bounces@lists.utsouthwestern.edu > 04/06/2007 09:27 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] Is the histonet working? > > > > > > > Hi--Happy Good Friday! Hope all of you have a Great > Easter Sunday. I haven't got any postings in the > last > 2 days. That's pretty unusual. Did I accidently > unsubscribe? > > Akemi Allison-Tacha > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From akemiat3377 <@t> yahoo.com Fri Apr 6 10:00:50 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Apr 6 10:00:58 2007 Subject: Fwd: Re: [Histonet] Is the histonet working? Message-ID: <973816.10006.qm@web31306.mail.mud.yahoo.com> --- Akemi Allison-Tacha wrote: > Date: Fri, 6 Apr 2007 07:49:53 -0700 (PDT) > From: Akemi Allison-Tacha > Subject: Re: [Histonet] Is the histonet working? > To: Jackie M O'Connor > CC: Histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > > OK-It's definitely Friday and I'll put on my > traveling > clothes and head home! Only thing is, it's 32 > degrees > out there! Boy do I miss that California weather!! > Can I have a second chance please? > Akemi > > > --- Jackie M O'Connor > wrote: > > > We voted you off the island, Akemi. > > > > > > > > > > > > Akemi Allison-Tacha > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 04/06/2007 09:27 AM > > > > To > > Histonet@lists.utsouthwestern.edu > > cc > > > > Subject > > [Histonet] Is the histonet working? > > > > > > > > > > > > > > Hi--Happy Good Friday! Hope all of you have a > Great > > Easter Sunday. I haven't got any postings in the > > last > > 2 days. That's pretty unusual. Did I accidently > > unsubscribe? > > > > Akemi Allison-Tacha > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From SBarnes <@t> elch.org Fri Apr 6 11:34:37 2007 From: SBarnes <@t> elch.org (Sue Barnes) Date: Fri Apr 6 11:35:04 2007 Subject: [Histonet] mammaglobin IHC Message-ID: Hi, Is there anyone who will share their protocol for mammaglobin to be used on the Nexus stainer. I am trying to get it up and running and have had no luck. Thanks Sue From LewisS <@t> pediatrics.ohio-state.edu Fri Apr 6 12:06:49 2007 From: LewisS <@t> pediatrics.ohio-state.edu (Lewis, Sarah) Date: Fri Apr 6 12:07:16 2007 Subject: [Histonet] Is the histonet working? In-Reply-To: Message-ID: <714B9F12B4E18C4C843B66E8E190F2AD01340B76@res2k3ms01.CRII.ORG> GOOD ONE!!! LOVE IT!!! -----Original Message----- From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: Friday, April 06, 2007 10:40 AM To: Akemi Allison-Tacha Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Is the histonet working? We voted you off the island, Akemi. Akemi Allison-Tacha Sent by: histonet-bounces@lists.utsouthwestern.edu 04/06/2007 09:27 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Is the histonet working? Hi--Happy Good Friday! Hope all of you have a Great Easter Sunday. I haven't got any postings in the last 2 days. That's pretty unusual. Did I accidently unsubscribe? Akemi Allison-Tacha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Fri Apr 6 12:18:47 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Apr 6 12:19:06 2007 Subject: [Histonet] Isopropyl has been used Message-ID: <8F3E1865E343C943BB38506D56FF01517F5299@MD1EV002.medimmune.com> There was an article done by folks at AFIP(I remembere cutting and working on this project), in the Journal of Histoechnology 10-15 years ago on something called clearing infiltration mixture. It is what u say, hot isopropyl, and a mixture of hot iso and paraffin before the full paraffin. It worked, lots of things "work", but a well-ventilated lab we determined at the AFIP to stick with xylene. It was better. The CIM worked, but xylene as the clearant was easier to cut blocks. The playing with the misture of the iso and the paraffin was a pain too, vendors will usually not warranty processors if you use that stuff either when the lines get clogged. I wish we had a better sub as well, but seems like after 30 yrs of this stuff I still go back to xylene. You could always use cedarwood oil........I am being funny, that is a great one, but is a neurotoxin and has to be cleared anyway. From akemiat3377 <@t> yahoo.com Fri Apr 6 12:20:32 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Apr 6 12:20:34 2007 Subject: Fwd: Re: [Histonet] Is the histonet working? Message-ID: <211745.7137.qm@web31301.mail.mud.yahoo.com> --- Akemi Allison-Tacha wrote: > Date: Fri, 6 Apr 2007 07:42:30 -0700 (PDT) > From: Akemi Allison-Tacha > Subject: Re: [Histonet] Is the histonet working? > To: Steven P Postl > > Yeah Steve! O'Hare is becoming my second home. I > feel like I should have wings for all the air miles > I've logged in for the past 4 weeks. 5 RT is a > little > too much. > Akemi > --- Steven P Postl > wrote: > > > It's been working. Maybe its the jet lag you are > > dealing with..... > > > > > > > > Akemi Allison-Tacha > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 04/06/2007 09:27 AM > > > > To > > Histonet@lists.utsouthwestern.edu > > cc > > > > Subject > > [Histonet] Is the histonet working? > > > > > > > > > > > > > > Hi--Happy Good Friday! Hope all of you have a > Great > > Easter Sunday. I haven't got any postings in the > > last > > 2 days. That's pretty unusual. Did I accidently > > unsubscribe? > > > > Akemi Allison-Tacha > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > From Rcartun <@t> harthosp.org Fri Apr 6 12:39:28 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 6 12:39:55 2007 Subject: [Histonet] mammaglobin IHC In-Reply-To: References: Message-ID: <46164D90020000770000528B@gwmail.harthosp.org> I can't help you with Nexus, but I use Dako's Mammaglobin mAb (M3625) on Vision BioSystems' Bond Max at a dilution of 1:500 with outstanding results. Please note that not all breast CAs show mammaglobin expression. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sue Barnes" 04/06/07 12:34 PM >>> Hi, Is there anyone who will share their protocol for mammaglobin to be used on the Nexus stainer. I am trying to get it up and running and have had no luck. Thanks Sue _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From carl.hobbs <@t> kcl.ac.uk Fri Apr 6 12:47:18 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Apr 6 12:47:31 2007 Subject: [Histonet] re: why do we use xylene? was - Peloris Processor and Message-ID: <002a01c77873$9f3b8a40$4101a8c0@carlba65530bda> In the late 70s I trialled isopropanol (IPA) as a processing dehydrant/"clearing" agent. It was completely successful for me for all my routine tinctorial/histochemical staining ( I wasn't using Immuno at that time, so cannot comment on that area). IPA is NOT miscible with wax at RT ( unlike xylene) but, at the temp. neccessary to melt your conventional waxes ( at least 60C) it is completely miscible. I mentioned this before...here, I think. I stopped using it as I subsequently took a lower post in Clinical labs and I was laughed at when I suggested testing IPA out. Great to read that it's come up again: perhaps I had also read Romeis' article in a journal, before it was published in a book? Too long ago to remember, lol. Carl From Rcartun <@t> harthosp.org Fri Apr 6 13:08:18 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 6 13:08:43 2007 Subject: [Histonet] mammaglobin IHC In-Reply-To: References: Message-ID: <461654520200007700005296@gwmail.harthosp.org> I can't help you with Nexus, but I use Dako's Mammaglobin mAb (M3625) on Vision BioSystems' Bond Max at a dilution of 1:500 with outstanding results. Please note that not all breast CAs show mammaglobin expression. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sue Barnes" 04/06/07 12:34 PM >>> Hi, Is there anyone who will share their protocol for mammaglobin to be used on the Nexus stainer. I am trying to get it up and running and have had no luck. Thanks Sue _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From gu.lang <@t> gmx.at Fri Apr 6 13:09:02 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Apr 6 13:08:55 2007 Subject: AW: [Histonet] re: why do we use xylene? was - Peloris Processor and In-Reply-To: <002a01c77873$9f3b8a40$4101a8c0@carlba65530bda> Message-ID: <000901c77876$a8d00e70$6412a8c0@dielangs.at> With a look in the isopropanol-datasheet it is to be seen, that this reagens is not really "safe". It has a flashpoint at 12?C. Perhaps not the ideal reagens to be used in electric instruments with hot temperatures? That's in my opinion the cause, why it wasn't generally used in the over-all VIPs. New developments could offer the necessary safety? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Carl Hobbs Gesendet: Freitag, 06. April 2007 19:47 An: Histonet Betreff: [Histonet] re: why do we use xylene? was - Peloris Processor and In the late 70s I trialled isopropanol (IPA) as a processing dehydrant/"clearing" agent. It was completely successful for me for all my routine tinctorial/histochemical staining ( I wasn't using Immuno at that time, so cannot comment on that area). IPA is NOT miscible with wax at RT ( unlike xylene) but, at the temp. neccessary to melt your conventional waxes ( at least 60C) it is completely miscible. I mentioned this before...here, I think. I stopped using it as I subsequently took a lower post in Clinical labs and I was laughed at when I suggested testing IPA out. Great to read that it's come up again: perhaps I had also read Romeis' article in a journal, before it was published in a book? Too long ago to remember, lol. Carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristopher.Kalleberg <@t> unilever.com Fri Apr 6 13:21:44 2007 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Fri Apr 6 13:21:53 2007 Subject: [Histonet] ready to use antibody Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C02314508@NTRSEVS30002.s3.ms.unilever.com> Has anyone ever used the ready to use antibodies which are prediluted? They are offerred by Lab Vision/Neomarkers. Is there any special protocol for these antibodies? I have run an experiment twice with this prediluted antibody and have had no results. Technical support was unable to help me with these items.Any help or information will be greatly appreciated. Thank you. Kris Kalleberg From rsrichmond <@t> aol.com Fri Apr 6 13:36:19 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Fri Apr 6 13:36:33 2007 Subject: [Histonet] Romeis - was why do we use xylene In-Reply-To: <200704061301.7aa46167cca357@rly-yi01.mx.aol.com> References: <200704061301.7aa46167cca357@rly-yi01.mx.aol.com> Message-ID: <8C946919A53CB58-CCC-6E59@webmail-mf05.sysops.aol.com> Barbara Bublava and Gudrun Lang mention Romeis, a reference book on technique, often cited by R.D. Lillie. I've never seen this book - checked amazon.de and it's out of print - do you think it's worth having a copy if you can read German? The book is Mikroscopische Technik, three postwar editions 1949, 1968, 1989, by Benno Romeis and Peter Boeck. Processing directly from isopropanol to paraffin? Just let me know when, and I'll announce my retirement! - since I turn 68 in a month - Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From gcallis <@t> montana.edu Fri Apr 6 13:39:17 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Apr 6 13:39:16 2007 Subject: [Histonet] Trying to contact Hiro Nitta Message-ID: <6.0.0.22.1.20070406123758.01b2e508@gemini.msu.montana.edu> Will Hiro Nitta please contact me. The last email address I have for him is Hiro_Nitta@ms.bd.com. Thank you Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From rjbuesa <@t> yahoo.com Fri Apr 6 14:34:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 6 14:34:27 2007 Subject: [Histonet] Romeis - was why do we use xylene In-Reply-To: <8C946919A53CB58-CCC-6E59@webmail-mf05.sysops.aol.com> Message-ID: <114349.46428.qm@web61217.mail.yahoo.com> Even when I don't read German, my copy of Romeis' book (Munchen 1948, from Leibniz Verlag, GDR) with a fantastic subject index and tables (with chemical names and amounts) have helped me in some occasions. A German-English dictionary also helps. Has many sections on almost any histology subject. The thing with isopropanol is that at 60?C forms an amulsion with the paraffin, although Peloris uses "burts of heat" to totally eliminate the propanol and let the paraffin to infiltrate. Isopropanol is mentioned as an antemedium also inBancroft and Stevens book. With relation to "dangerousness" propanol has a TWA of 200 ppm, which makes it twice less toxic than xylene or naphtha. Regarding flash points, for pure iso-propanol = 12?C, for pure ethanol = 14?C and I don't think that a 2?C difference makes for much! Xylene flash point = 25?C There is a paper by Falkeholm at al. (Lab.Invest. 81(9):1213-21; 2001), with very good statistical support, where they demonstrate that propanol is as good or better as an antemedium than xylene for paraffin infiltration (they even eliminated ethanol and dehydrate with propanol). The study was conducted in Sweden. Ren? J. rsrichmond@aol.com wrote: Barbara Bublava and Gudrun Lang mention Romeis, a reference book on technique, often cited by R.D. Lillie. I've never seen this book - checked amazon.de and it's out of print - do you think it's worth having a copy if you can read German? The book is Mikroscopische Technik, three postwar editions 1949, 1968, 1989, by Benno Romeis and Peter Boeck. Processing directly from isopropanol to paraffin? Just let me know when, and I'll announce my retirement! - since I turn 68 in a month - Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From CIngles <@t> uwhealth.org Fri Apr 6 14:54:01 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Apr 6 14:54:06 2007 Subject: [Histonet] ready to use antibody References: <0E6BC087F70F9C47ACFF2C203D6E329C02314508@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120020@uwhis-xchng3.uwhis.hosp.wisc.edu> Kris: I have been playing on getting some immunos up and running for our Mohs lab (so frozen skin sample only). I have found the Biocare kits work great. I use the Alk. Phos. kit with a fast red chromogen (permanent, not aqueous) I have tried the aqueous fast red Alk. Phos. kit from Innovex and it doesn't work for me. I've tried everything I can think of. The kit from Biocare worked the first time I tried it. They also have great customer service with tons of tech support. Hope this helps. I know how frustrating this stuff can be. Good Luck. Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kalleberg, Kristopher Sent: Fri 4/6/2007 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ready to use antibody Has anyone ever used the ready to use antibodies which are prediluted? They are offerred by Lab Vision/Neomarkers. Is there any special protocol for these antibodies? I have run an experiment twice with this prediluted antibody and have had no results. Technical support was unable to help me with these items.Any help or information will be greatly appreciated. Thank you. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Fri Apr 6 15:38:32 2007 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Fri Apr 6 15:40:21 2007 Subject: [Histonet] (no subject) Message-ID: Hi All, We are wondering how others are testing temperature reproducibility on their microwaves. We are using a plastic coplin jar filled with distilled water. We then heat it at different powers and times and take the temperature with a thermometer. Any suggestions for a better way? Thank you. Sally Norton Histology Seattle Children's Hospital and Regional Medical Center CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Rcartun <@t> harthosp.org Fri Apr 6 15:41:20 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 6 15:41:34 2007 Subject: [Histonet] ready to use antibody In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C02314508@NTRSEVS30002.s3.ms.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C02314508@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <4616783002000077000052A0@gwmail.harthosp.org> We don't use many "ready-to-use" (RTU) antibodies, but we have found that we can usually dilute the ones that we do use 1:5, 1:10, 1:20, 1:50, or maybe even 1:100 for optimal staining. Obviously, the dilution will be determined by the sensitivity of your detection system and the length of your primary antibody incubation. Have you tried different tissue pretreatments? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Kalleberg, Kristopher" 04/06/07 2:21 PM >>> Has anyone ever used the ready to use antibodies which are prediluted? They are offerred by Lab Vision/Neomarkers. Is there any special protocol for these antibodies? I have run an experiment twice with this prediluted antibody and have had no results. Technical support was unable to help me with these items.Any help or information will be greatly appreciated. Thank you. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From apothad <@t> gmail.com Fri Apr 6 17:37:48 2007 From: apothad <@t> gmail.com (Adam Kirk) Date: Fri Apr 6 17:37:51 2007 Subject: [Histonet] PLease UNSUBSCRIBE ME!!! Message-ID: <40e76ce80704061537s3347d14ay6b69e4aa83c840d2@mail.gmail.com> Dear Histonet, I would be grateful if you could take me off your mailing list. I have tried contacting you three times now to no avail. PLEASE REMOVE ME. PLEASE!!! Adam -- Dr Adam Kirk MRCP Renal/GIM Registrar 07966015047 From gu.lang <@t> gmx.at Sat Apr 7 01:26:20 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Apr 7 01:26:07 2007 Subject: AW: [Histonet] Romeis In-Reply-To: <8C946919A53CB58-CCC-6E59@webmail-mf05.sysops.aol.com> Message-ID: <000501c778dd$a8470280$6412a8c0@dielangs.at> Dear Bob, The Romeis is something like the "histo-bible" in german speaking countries. You can find it in every histolab, but it is really sad, that it is out of print. The last edition from 1989 doesn't describe modern methods (ihc, in situ) for the today's need. But it is the biggest collection of histochemical methods I know (in German). So if you can get one, get it (and sell it for the 10fold price). I hope the editors of the Romeis will produce a modern edition. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von rsrichmond@aol.com Gesendet: Freitag, 06. April 2007 20:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Romeis - was why do we use xylene Barbara Bublava and Gudrun Lang mention Romeis, a reference book on technique, often cited by R.D. Lillie. I've never seen this book - checked amazon.de and it's out of print - do you think it's worth having a copy if you can read German? The book is Mikroscopische Technik, three postwar editions 1949, 1968, 1989, by Benno Romeis and Peter Boeck. Processing directly from isopropanol to paraffin? Just let me know when, and I'll announce my retirement! - since I turn 68 in a month - Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Apr 7 01:37:09 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Apr 7 01:36:56 2007 Subject: AW: [Histonet] ready to use antibody In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C02314508@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <000601c778df$2b8253b0$6412a8c0@dielangs.at> Prediluted antibodies are usually designed on the detection system of the company (or partners) to get best results. With your own detection system who have to try modifications as you do it with the antibody-concentrates. For example we use the prediluted Ventana-Antibodies on the Benchmark as they are, and RTU-antibodies from other suppliers in 1:2, or 1:3 dilutions. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kalleberg, Kristopher Gesendet: Freitag, 06. April 2007 20:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] ready to use antibody Has anyone ever used the ready to use antibodies which are prediluted? They are offerred by Lab Vision/Neomarkers. Is there any special protocol for these antibodies? I have run an experiment twice with this prediluted antibody and have had no results. Technical support was unable to help me with these items.Any help or information will be greatly appreciated. Thank you. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat Apr 7 11:33:01 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Apr 7 11:33:07 2007 Subject: [Histonet] (no subject) References: Message-ID: <001f01c77932$6915cab0$11614542@yourxhtr8hvc4p> Sally, I'm using a Pyrex beaker and changing the distilled water each time. I set my times by the times used for the special stains. For instance, my lowest time in the microwave is 15 seconds, my highest time is 45 seconds. I set up a chart with 15, 30, and 45 seconds. I run this three times for each time and get an average. When I had my inspection in January, the inspector took a copy of my procedure and chart with her. Again, each inspector is different, but it passed. I'd be happy to share and I promise to keep my toes to myself. JTT ----- Original Message ----- From: "Norton, Sally" To: Sent: Friday, April 06, 2007 3:38 PM Subject: [Histonet] (no subject) Hi All, We are wondering how others are testing temperature reproducibility on their microwaves. We are using a plastic coplin jar filled with distilled water. We then heat it at different powers and times and take the temperature with a thermometer. Any suggestions for a better way? Thank you. Sally Norton Histology Seattle Children's Hospital and Regional Medical Center CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Apr 7 12:24:03 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Apr 7 12:24:00 2007 Subject: [Histonet] re: why do we use xylene? was - Peloris Message-ID: <001e01c77939$8a40fc80$4101a8c0@carlba65530bda> I agree with you, Gudrun.... Not about flashpoints( I would be much more worried about Xylene) but about IPA's use in automated, enclosed processors. I have no Xperience of this...I used IPA in a conventional dipndunk automated processor. Carl The Fire Starter...;-) From Rrlopez2 <@t> aol.com Sat Apr 7 13:38:34 2007 From: Rrlopez2 <@t> aol.com (Rrlopez2@aol.com) Date: Sat Apr 7 13:38:40 2007 Subject: [Histonet] Ventana, Symphony Stainer Message-ID: Just wanted to know if anyone is using the new Symphony Stainer from Ventana, if yes what do you like or dislike about it. Thanks ************************************** See what's free at http://www.aol.com. From Tilman.Fuldatal <@t> web.de Sat Apr 7 17:25:31 2007 From: Tilman.Fuldatal <@t> web.de (Tilman Krieger) Date: Sat Apr 7 17:25:38 2007 Subject: [Histonet] please unsubscribe Message-ID: <1789798184@web.de> please unsubscribe me from the mailing list. thanks. Til MR _______________________________________________________________ SMS schreiben mit WEB.DE FreeMail - einfach, schnell und kostenguenstig. Jetzt gleich testen! http://f.web.de/?mc=021192 From doug <@t> ppspath.com Mon Apr 9 08:10:47 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Apr 9 07:09:04 2007 Subject: [Histonet] Ventana, Symphony Stainer In-Reply-To: Message-ID: I use the Symphony. Are you from that company that is trying to get "consultations" for Ventana competitors or are you a serious inquiring facility? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rrlopez2@aol.com Sent: Saturday, April 07, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana, Symphony Stainer Just wanted to know if anyone is using the new Symphony Stainer from Ventana, if yes what do you like or dislike about it. Thanks ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Mon Apr 9 07:28:41 2007 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Mon Apr 9 07:28:50 2007 Subject: [Histonet] Laser microwriter Message-ID: Am interested in some information from users of the Thermo Microwriter. Could I have some feed back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD From lesley.bechtold <@t> jax.org Mon Apr 9 07:55:10 2007 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Mon Apr 9 07:55:38 2007 Subject: [Histonet] Position for Histotechnologist in Bar Harbor, ME available Message-ID: <20070409085510458.00000001608@spikey> There is a fulltime position in the Histology Service of The Jackson Laboratory as a Histotechnologist II/III. Responsibilities include conduct of standard histological protocols including embedding, sectioning and staining as well as routine laboratory maintenance and administrative tasks. Minimum qualifications include Associate's degree in a biological science and HT(ASCP) certification plus 2 years of experience in histology OR a Bachelor's degree in a biological field and 2-3 years of experience in histology. Experience in murine histology and specialized techniques such as serial sectioning, immunohistochemistry, plastic embedding and plastic sectioning is helpful. The incumbent will have the opportunity to further their skills and knowledge. Required computer skills include email, internet, word processing, spreadsheets and familiarity with databases. Effective written and verbal communication skills are essential. Successful applicants will demonstrate good interpersonal skills and must have the ability and willingness to function effectively in a team environment. The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia National Park. Mountains, ocean, forests, lakes, and trails are all within walking distance. If you are looking for a more natural environment, this could be the opportunity you've been searching for. Interested individuals should apply on-line on the internet at www.jax.org, click on Careers in the green bar at the top of the page, then on Search Job Listings in the right-hand column. Refer to position # jax-00000721. Please submit cover letter and resume as one document. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From ROrr <@t> enh.org Mon Apr 9 08:02:06 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Apr 9 08:02:11 2007 Subject: [Histonet] using predilutes Message-ID: When I have encountered a prediluted antibody that works best when I dilute it out further, I go ahead and just find the concentrate and dilute out myself. I hesitate adding more diluent to a product that already has been diluted. Granted even our concentrates are not "full strength" even concentrates are diluted by the time they get to us. But for me in a clinical lab, I believe that if a predilute must be diluted out further, why bother with it? I am adding another variable to the mixture. I don't know what diluent the manufacturer is using...what pH, whether it's pbs or not... what preservatives... ok ok, yes I can look at the data sheet and test the pH of the predilute to figure all that out...like I said, why bother? I'd have to have another type of validation to justify diluting out this type of product. I'd have to somehow document why and how I am not following manufacturer's recommendations on a prediluted product... or am I just in over kill mode here? If a predilute is over staining, I decide if I want to back off incubation time..rethink my detection or check over the HIER. I ran into this with the prediluted SMA from Biocare...it was overstaining everything! I suspect it's the Mach 4 that is just crazy hot... Plus I was running HIER... once I actually READ the data sheet, I saw that they don't recommend HIER! So, with that said, I ended up buying the concentrate and running it about 1:500 NO HIER. All better now. I guess the point I'm trying to make is to keep trying when you get over stained results or no results...sometimes it's just a matter of combining the right detection/antibody. Maybe since I've been in tech support I understand how valuable the manufacturer's tech support staffers really are in helping out in these situations. Ok I'll stop now...I've had way too much coffee this morning. Becky Orr Evanston Hospital Message: 14 Date: Fri, 06 Apr 2007 16:41:20 -0400 From: "Richard Cartun" Subject: Re: [Histonet] ready to use antibody To: , "Kristopher Kalleberg" Message-ID: <4616783002000077000052A0@gwmail.harthosp.org> Content-Type: text/plain; charset=US-ASCII We don't use many "ready-to-use" (RTU) antibodies, but we have found that we can usually dilute the ones that we do use 1:5, 1:10, 1:20, 1:50, or maybe even 1:100 for optimal staining. Obviously, the dilution will be determined by the sensitivity of your detection system and the length of your primary antibody incubation. Have you tried different tissue pretreatments? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Kalleberg, Kristopher" 04/06/07 >>> 2:21 PM >>> Has anyone ever used the ready to use antibodies which are prediluted? They are offerred by Lab Vision/Neomarkers. Is there any special protocol for these antibodies? I have run an experiment twice with this prediluted antibody and have had no results. Technical support was unable to help me with these items.Any help or information will be greatly appreciated. Thank you. From mjlco <@t> aol.com Mon Apr 9 08:50:07 2007 From: mjlco <@t> aol.com (mjlco@aol.com) Date: Mon Apr 9 08:50:22 2007 Subject: [Histonet] McMannas PAS Light Green In-Reply-To: References: Message-ID: <8C948C51E1CD2F1-14B4-5C6@WEBMAIL-RA04.sysops.aol.com> Morning Histonetters, I am in need of everyones help. I am doing a McMannas PAS with light green on a fungus but am un-sure how much the light green it to show. Every time I have done this stain so far the light green has been extremly faint. I have been using a fungus in lung control. I have also been unable to find a photo of what this stain should look like. Thanks for all of your Help. Matt Lunetta Longmont United Hospital ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Charlene.Henry <@t> STJUDE.ORG Mon Apr 9 08:56:59 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Apr 9 08:57:03 2007 Subject: [Histonet] Ventana, Symphony Stainer. . In-Reply-To: Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A20AA@SJMEMXMB02.stjude.sjcrh.local> We are using the Symphony and love it. The techs love it because they do not have to rotate reagents and it is so easy to use. The quality of staining is excellent and this keeps the pathologists happy. I'll have to say that we are very happy with our Symphony. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rrlopez2@aol.com Sent: Saturday, April 07, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana, Symphony Stainer. . Just wanted to know if anyone is using the new Symphony Stainer from Ventana, if yes what do you like or dislike about it. Thanks ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eileen.lonergan <@t> verizon.net Mon Apr 9 10:41:19 2007 From: eileen.lonergan <@t> verizon.net (eileen.lonergan@verizon.net) Date: Mon Apr 9 10:42:06 2007 Subject: [Histonet] Job Openings New England Message-ID: <10402778.7622601176133279345.JavaMail.root@vms070.mailsrvcs.net> Hi Histonetters, We have taken on additional clients and services over the last few months and need to fill our rapidly expanding Histology Department. We are a large reference lab servicing 8 large suburban hospitals. We are located on Boston?s North Shore in Peabody MA, just off Route One North and 30 minutes from the NH border. Our salaries are very competitive with Boston salaries without the hassle of the commute and parking. State of the Art, newly designed lab with great benefits and an even greater group of experienced Histotechs. Openings are for 2nd and 3rd shift at the moment, but an early 1st shift start time (3am) is not out of the question for the right person. Please contact me directly either by email or phone. Expertise in human tissue is a must as well as biopsy embedding and microtomy. Remember, Summer in New England is just around the corner!! Eileen A Lonergan, HT(ASCP) Histopathology Manager ConVerge Diagnostic Services CytoDx Histology Division 200 Corporate Place, Suite 7 Peabody, MA 01960 (978) 548-5206 Office (207) 749-9617 Cell (978) 536-5173 Histology Fax http://www.convergedx.com mailto:elonergan@convergedx.com Eileen Lonergan (207) 749-9617 cell (207) 324-6468 home From Charlene.Henry <@t> STJUDE.ORG Mon Apr 9 10:59:03 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Apr 9 10:59:08 2007 Subject: [Histonet] Gli 1 Antibody Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A20AD@SJMEMXMB02.stjude.sjcrh.local> Good Morning Fellow Histonetters, I was wondering if any of you have the antibody Gli-1 working in FFPE tissues? If so where did you purchase your antibody and what are you using as a control? I have tried the Gli-1 cat # ab7523 from Abcam and Gli-1 cat. # 100-401-223 from Rockland without any luck. Thanks, Charlene From EWURDAK <@t> CSBSJU.EDU Mon Apr 9 11:27:57 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Mon Apr 9 11:28:04 2007 Subject: [Histonet] Plastic sections Message-ID: Hello Histonetters, We are having trouble with the JB-4 embedding medium. No matter how carefully we follow instructors provided by the vendor, EMS, our blocks turn out to be too hard to section with sharp glass knives. Should we cut back on the catalyst, soak the blocks or switch to a different embedding medium altogether? Any help you can give me will be much appreciated. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 From jmahoney <@t> alegent.org Mon Apr 9 11:28:59 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Mon Apr 9 11:29:18 2007 Subject: [Histonet] Employment Message-ID: <461A237B0200003C0000A7DA@gwia.alegent.org> Hello Histonetters, Please pass this on to anyone who may be interested. We have an immediate opening in our Histology lab. Part time or Full time can be negotiated. Please join our "World Class" LEAN laboratory. We have a dedicated, highly motivated, fun Histology Team. Alegent Health, located in Omaha, Nebraska, offers a full benefit package. We prefer HT(ASCP) registered but will consider those moving toward their certification. Apply online at: https://www.alegent.com We are an equal opportunity employer. Jan Mahoney From MSHERWOOD <@t> PARTNERS.ORG Mon Apr 9 11:36:35 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Apr 9 11:36:43 2007 Subject: [Histonet] Plastic sections In-Reply-To: Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A310EA@PHSXMB1.partners.org> Liz, I've never worked with JB-4, but soaking epon/epoxy blocks doesn't work as with paraffin. What you need to play with is the hardner component of the mix. I know that sometimes other epoxys are needed for certain procedures, but my experience is with Epon 812, which I prefer. I am an electron microscopist, so I work exclusively with plastic sections. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wurdak, Elizabeth Sent: Monday, April 09, 2007 12:28 PM To: Histonet Subject: [Histonet] Plastic sections Hello Histonetters, We are having trouble with the JB-4 embedding medium. No matter how carefully we follow instructors provided by the vendor, EMS, our blocks turn out to be too hard to section with sharp glass knives. Should we cut back on the catalyst, soak the blocks or switch to a different embedding medium altogether? Any help you can give me will be much appreciated. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From ruebenjcarter <@t> gmail.com Mon Apr 9 11:58:42 2007 From: ruebenjcarter <@t> gmail.com (R C) Date: Mon Apr 9 11:58:46 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 In-Reply-To: <46152c26.3d4c03d6.566b.ffffb07dSMTPIN_ADDED@mx.google.com> References: <46152c26.3d4c03d6.566b.ffffb07dSMTPIN_ADDED@mx.google.com> Message-ID: <2a926e3f0704090958p7f22457n47ef2e2f329e5ae1@mail.gmail.com> Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > From gcallis <@t> montana.edu Mon Apr 9 12:15:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Apr 9 12:15:06 2007 Subject: Hard GMA plastic Re: [Histonet] Plastic sections In-Reply-To: References: Message-ID: <6.0.0.22.1.20070409105628.01b509e8@gemini.msu.montana.edu> Elizabeth, Don't adjust the catalyst i.e Benzoyl peroxide - you need to keep that concentration the same. You need to add MORE plasticizer - the nn dimethyl aniline - that should soften the plastic or make it more pliable for sectioning. Soaking a GMA block is not recommended, as the fumes or getting any of the wetted plastic on you is a toxic problem. Using a hot water bath to flatten sections also causes fume release. I have several colleagues who are totally sensitized to GMA plastic and cannot be in a room where GMA fumes exist. I also suggest wearing safety glasses when sectioning any GMA, to prevent a section from flying into the eye on a static filled day - a problem one lady encountered. You could also put your blocks in a moist atmosphere to let them humidify a bit and soften the plastic. This may be easier than trying to adjust the plasticizer component. An alternative to GMA is use Technovits 7100 - another GMA kit which many really like. Histonet archives will have discussion on these kits, and also more on softening the polymerized blocks. Good luck At 10:27 AM 4/9/2007, you wrote: >Hello Histonetters, >We are having trouble with the JB-4 embedding medium. No matter how >carefully we follow instructors provided by the vendor, EMS, our blocks turn >out to be too hard to section with sharp glass knives. Should we cut back >on the catalyst, soak the blocks or switch to a different embedding medium >altogether? >Any help you can give me will be much appreciated. >Liz > >Elizabeth Wurdak, PhD >Biology Department >Saint John?s University >Collegeville, MN 56321 >Tel: (320) 363-3177 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From rsrichmond <@t> aol.com Mon Apr 9 12:29:01 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Mon Apr 9 12:29:09 2007 Subject: [Histonet] Re: McManus ("McMannas") PAS Light Green In-Reply-To: <200704091300.977461a7148293@rly-mf08.mail.aol.com> References: <200704091300.977461a7148293@rly-mf08.mail.aol.com> Message-ID: <8C948E3B27DAB95-CCC-B8EE@webmail-mf05.sysops.aol.com> Matt Lunetta at Longmont United Hospital asks: >> I am doing a McMannas [sic] PAS with light green on a fungus but am unsure how much of the light green it is to show. Every time I have done this stain so far the light green has been extremely faint. - I have been using a fungus (in lung) as a control. I have been unable to find a photo of what this stain should look like.<< The PAS stain (McManus, about 1946) when used for fungi is indeed often counterstained with light green SF (or the closely related fast green FCF). The green can be quite faint, as long as you can see the nuclei well enough to orient yourself. If the green is too dense, it may make the fungi a little hard to see. This color combination can cause problems for the pathologist (one man in eight) who is red-green color-blind. Trivia time: the "FCF" after fast green supposedly stands for "for coloring food". Like other triarylmethyl dyes, fast green is probably a potent carcinogen. That was a LONG time ago! Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From doug <@t> ppspath.com Mon Apr 9 13:42:08 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Apr 9 12:41:43 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 In-Reply-To: <2a926e3f0704090958p7f22457n47ef2e2f329e5ae1@mail.gmail.com> Message-ID: Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Mon Apr 9 13:07:31 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Apr 9 13:07:50 2007 Subject: [Histonet] bouins fixed mouse tissue Message-ID: Dear Histonetters, Has anyone experienced chatter artifact while sectioning Bouins fixed mouse tissue? The tissue is fixed in Bouins fixative for 24 hrs. and transferred to 70% EtOH for rinsing. The tissues are processed 15 min. at each solution station. Helen Johnson (hej01@health.state.ny.us.) From algranth <@t> u.arizona.edu Mon Apr 9 14:25:01 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Apr 9 14:25:18 2007 Subject: [Histonet] bouins fixed mouse tissue In-Reply-To: Message-ID: <4.3.2.7.2.20070409122058.0c1425e8@algranth.inbox.email.arizona.edu> helen, What kind of tissue are you trying to cut? I frequently get mouse ovaries fixed for 24 hrs in Bouins and transfered to 70% ETOH. They usually cut fine but in your case my suggestion is SOAK, SOAK, SOAK in icy water. Not long ago someone here suggested putting a little glycerin in the soaking water - you can find the actual posts on the archives - but I tried it and was delighted with the results. Andi Grantham At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote: >Dear Histonetters, > Has anyone experienced chatter artifact while sectioning Bouins >fixed mouse tissue? The tissue is fixed in Bouins fixative for 24 hrs. and >transferred to 70% EtOH for rinsing. The tissues are processed 15 min. at >each solution station. >Helen Johnson (hej01@health.state.ny.us.) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From akemiat3377 <@t> yahoo.com Mon Apr 9 14:48:31 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Apr 9 14:48:38 2007 Subject: [Histonet] bouins fixed mouse tissue In-Reply-To: <4.3.2.7.2.20070409122058.0c1425e8@algranth.inbox.email.arizona.edu> Message-ID: <103106.48881.qm@web31315.mail.mud.yahoo.com> Helen, As you probably know, mouse tissue is much more delicate than human tissue. If you have control over the fixation process, I would cut down the initial fixation time in Bouin's solution. Perhaps, try 6-12 hours. For the tissue that you have already processed, soak in a tween 80 and water mixture of 1:10 for a minimum of 10 minutes, followed by a water wash to remove tween, then soak on a ice tray. This works beautifully for over-processed tissue, skin, uterus, ect. PS: Tween 20 can be substituted or a similar surfactant. Good Luck, Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Andrea Grantham wrote: > helen, > What kind of tissue are you trying to cut? > I frequently get mouse ovaries fixed for 24 hrs in > Bouins and transfered to > 70% ETOH. They usually cut fine but in your case my > suggestion is SOAK, > SOAK, SOAK in icy water. > Not long ago someone here suggested putting a little > glycerin in the > soaking water - you can find the actual posts on the > archives - but I tried > it and was delighted with the results. > > Andi Grantham > > > At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote: > > >Dear Histonetters, > > Has anyone experienced chatter artifact > while sectioning Bouins > >fixed mouse tissue? The tissue is fixed in Bouins > fixative for 24 hrs. and > >transferred to 70% EtOH for rinsing. The tissues > are processed 15 min. at > >each solution station. > >Helen Johnson (hej01@health.state.ny.us.) > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell > Biology & Anatomy : > : Sr. Research Specialist University of > Arizona : > : (office: AHSC 4212) P.O. Box 245044 > : > : (voice: 520-626-4415) Tucson, AZ > 85724-5044 USA : > : (FAX: 520-626-2097) (email: > algranth@u.arizona.edu) : > :...................................................................: > > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From susanbachus <@t> verizon.net Mon Apr 9 15:54:23 2007 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Mon Apr 9 15:54:35 2007 Subject: [Histonet] need a sliding/freezing microtome References: <4D14F0FC9316DD41972D5F03C070908B0DB461@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <000701c77ae9$40fbc940$2e01a8c0@RESLAPTOP> If anyone out there has an old (but functional [cosmetic appearance not a concern]) sliding/freezing microtome collecting dust that you would like to sell, we suddenly & urgently need one (and also have some money to pay for one, fortunately). Either the old fashioned kind with a tray for dry ice or the newer "cryo-electronic" freezing kind would be fine. We're in N. Virginia so if there's a lab in the DC/MD/VA area interested in selling one, I could probably drive to where you are to pick it up. Thanks for your consideration! Susan From jnocito <@t> satx.rr.com Mon Apr 9 18:23:25 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Apr 9 18:23:44 2007 Subject: [Histonet] McMannas PAS Light Green References: <8C948C51E1CD2F1-14B4-5C6@WEBMAIL-RA04.sysops.aol.com> Message-ID: <012101c77afe$12fcaea0$11614542@yourxhtr8hvc4p> Matt, we perform a PAS/Fungus on all toenails we gross (hence Joe the Toe). The doctors I work for like out light green strong. I can't count how many times they kicked back trays of slides that weren't stained dark enough. We have a TissueTek stain line that we use for our special stains. We have an extra container filled with 95% ETOH we use to rinse off the Fast Green. Every time we did a quick rinse in water, the green always washed out. Personally, the magenta color with the dark green just curls my toes up. I'm thinking of painting my living room with those colors. JTT ----- Original Message ----- From: To: Sent: Monday, April 09, 2007 8:50 AM Subject: [Histonet] McMannas PAS Light Green > Morning Histonetters, > > I am in need of everyones help. I am doing a McMannas PAS with light green > on a fungus but am un-sure how much the light green it to show. Every time > I have done this stain so far the light green has been extremly faint. > > I have been using a fungus in lung control. > > I have also been unable to find a photo of what this stain should look > like. > > Thanks for all of your Help. > Matt Lunetta > Longmont United Hospital > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCarpenter764 <@t> aol.com Mon Apr 9 18:39:28 2007 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Mon Apr 9 18:39:36 2007 Subject: [Histonet] cryostat question Message-ID: So today I had a sentinel node from the OR. I had many frozen sections on t his case but couldn't seem to get a good section. I couldn't even cut a perfect section without it chunking my block. Here are a couple things I noticed. Our frozen section room was extremely hot...I would say about 85 degrees. I had to make all knew chucks b/c the one's in the cryostat's were old. we had previously opened bottles of oct. They all had condensation in them. The chucks that I made had been sitting at room temperature. I need some help troubleshooting the fact that with both cryostat's the 1800 and the 1850 keep biteing into my chuck without advancing it. Please help...thanks Jennell ************************************** See what's free at http://www.aol.com. From JCarpenter764 <@t> aol.com Mon Apr 9 18:41:22 2007 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Mon Apr 9 18:41:48 2007 Subject: [Histonet] Fwd: cryostat question Message-ID: ************************************** See what's free at http://www.aol.com. From mickie25 <@t> netzero.net Mon Apr 9 19:59:45 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Mon Apr 9 20:00:25 2007 Subject: [Histonet] cryostat question In-Reply-To: Message-ID: Hi Jennell, I teach technicians to cut Mohs frozen sections every week and on Reichert-Jung and Leica cryostats, and can offer some advice re: your problems: 1. Your cryostat is designed to operate in a room temp range of from 68 - 72 degrees F, optimal. So the tissue was possibly not cold enough. Also make sure the vents on the left and right side of the cryostat are unobstructed so the compressor coils will work as efficiently as possible. Is the defrost time set to midnight or there about? 2. Build-ups (pre frozen OCT on a chuck) should be made fresh each morning. They should be made one at a time so the chuck is not too cold when the OCT is applied and can run down into the grooves completely. 3. Build-ups over a day old have freeze-dried and so the new OCT applied does not stick well to the old dried out stuff on the chuck and the OCT on the chuck has dried and shrunken somewhat and does not hold well to the chuck. 4. The knife angle that seems to work best for me is 2.5 degrees rather than the 5 degrees often set by Leica reps and from the factory. I have encountered knife angles everywhere from zero to 10 degrees. 5. Everything from the clamping of the knife holder to the microtome base, angle clamp, knife lateral adjustment clamp and knife clamp should be very tight with no movement at all. Looseness here and with the chuck can lead to chatter which will eventually lead to chunking of the block. Cut slower. 6. The screw clamping the chuck into the microtome and the chuck angle clamp should also be tight. 7. If the OCT shows condensation of moisture inside the bottle, then it was probably less hydrated and this would affect cutting consistency, but this would be minor compared to the above. This could be because the room is so hot. This is what comes to mind but without being there, this does not rule out something major wrong with the microtome itself. Also, all clamps on the knife holder should tighten toward the user except for the knife blade clamp which most people push toward the back from vertical. Lymph node usually tends to shatter when cut even at minus 20 degrees. Warming the face slightly with the thumb before taking the section may help this a little. Knife angle is also a problem if too steep. Hope this helps a little. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JCarpenter764@aol.com Sent: Monday, April 09, 2007 4:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat question So today I had a sentinel node from the OR. I had many frozen sections on t his case but couldn't seem to get a good section. I couldn't even cut a perfect section without it chunking my block. Here are a couple things I noticed. Our frozen section room was extremely hot...I would say about 85 degrees. I had to make all knew chucks b/c the one's in the cryostat's were old. we had previously opened bottles of oct. They all had condensation in them. The chucks that I made had been sitting at room temperature. I need some help troubleshooting the fact that with both cryostat's the 1800 and the 1850 keep biteing into my chuck without advancing it. Please help...thanks Jennell ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Apr 10 07:07:52 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 10 07:08:11 2007 Subject: [Histonet] Bone saw Message-ID: <461B45D80200007700005337@gwmail.harthosp.org> We need a good saw for cutting thin slices of bone (4-5 mm.) to submit for microscopic evaluation following decalcification. Any suggestions? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From nena.dimaano <@t> roche.com Tue Apr 10 07:17:28 2007 From: nena.dimaano <@t> roche.com (Dimaano, Nena) Date: Tue Apr 10 07:17:42 2007 Subject: [Histonet] Histology Graduate Course On-line Message-ID: <5034B118F526E547A9B897721075839305DCC7D1@rnumsem02.nala.roche.com> Hi All, I was wondering if anyone knows of Histology Graduate Level Course On-line. I appreciate your help. Best Regards, Nena *********************************** Nena Dimaano Non Clinical Drug Safety Hoffmann La Roche 340 Kingsland Ave. Nutley, NJ 07110 tel: 973-235-3953 fax: 973-235-4710 email: Nena.Dimaano@roche.com *********************************** From jqb7 <@t> cdc.gov Tue Apr 10 07:21:48 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Apr 10 07:22:22 2007 Subject: [Histonet] Histology Graduate Course On-line References: <5034B118F526E547A9B897721075839305DCC7D1@rnumsem02.nala.roche.com> Message-ID: Nebraska State University has a graduate level on-line histology course about to start. Go to www.unk.edu to check it out. You do not have to be enrolled in the Graduate program to take it. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dimaano, Nena Sent: Tuesday, April 10, 2007 8:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Graduate Course On-line Hi All, I was wondering if anyone knows of Histology Graduate Level Course On-line. I appreciate your help. Best Regards, Nena *********************************** Nena Dimaano Non Clinical Drug Safety Hoffmann La Roche 340 Kingsland Ave. Nutley, NJ 07110 tel: 973-235-3953 fax: 973-235-4710 email: Nena.Dimaano@roche.com *********************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Apr 10 07:35:52 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 10 07:35:50 2007 Subject: [Histonet] RELIA'S Histology Opportunity Update 4-10-07 Message-ID: Good Morning Histonetters, I just wanted to drop you a line and tell you about my current positions. All of the positions I work with are fulltime 40 hour per week positions with top hospitals, and labs. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance and in some cases sign on bonuses. These are all first shift or day shift positions unless otherwise noted. My services are FREE of charge to you. All of my fees are paid by my clients, (the facilities that I represent). I represent companies nationwide that are in need of histology supervisors, histotechnologists and histo technicians. Here is a list of my most exciting current openings: HISTOLOGY MANAGEMENT: Histology Manager ? Greater Boston Area Histology Manager ? Central Florida IHC Supervisor ? Southern CA Histology Supervisor ? South Texas (For your coworkers in Cytology I have an opening for a cytology supervisor in PA) HISTOLOGY TECHNICIAN/TECHNOLOGIST Histo Tech ? South Texas Histo Tech ? Rhode Island Histo Tech/PA ? North Central Florida Histo Tech ? Southwest Florida Histo Tech ? Illinois Vendor Rep ? Texas Histo Tech 3rd shift ? OH If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. If you know of anyone who would like to receive my e-mail as well please feel free to send me their contact information and I would be happy to send it to them as well. Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From mari.ann.mailhiot <@t> leica-microsystems.com Tue Apr 10 08:27:20 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Apr 10 08:27:35 2007 Subject: [Histonet] cryostat question In-Reply-To: Message-ID: Hi Jennell The first and foremost concern is the temperature in the room. 85 degrees is very warm for a cryostat. The cryostat has to work very hard to keep up with the outside room temperature. If there is a way to get the room cooler that would help. If not would it be possible to move the unit to someplace cooler? I know I may be asking for the impossible but I am concerned about the temperature in the room. There may be many reasons why the block is chunking. Are you adding the OCT to a warm or a cold chuck? It is better to add the OCT to a warm chuck. Then the chuck and OCT equilibrate to the temperature in the cryostat at the same time. If the chuck is cold or has a base of OCT that has been frozen already and you add warm OCT sometimes the OCT doesn't hold very well to the cold block or the cold OCT. Therefore there maybe chunking into the block. Since the room is very warm, it would probably be easier to only keep one or two bottle of OCT open at one time. Your knife holder may also have something loose, or the block holder may be loose. You also may need preventative maintenance on the cryostats. Please give me a call and we can discuss some of your issues in more detail. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com JCarpenter764@aol .com Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] cryostat question 04/09/2007 06:39 PM So today I had a sentinel node from the OR. I had many frozen sections on t his case but couldn't seem to get a good section. I couldn't even cut a perfect section without it chunking my block. Here are a couple things I noticed. Our frozen section room was extremely hot...I would say about 85 degrees. I had to make all knew chucks b/c the one's in the cryostat's were old. we had previously opened bottles of oct. They all had condensation in them. The chucks that I made had been sitting at room temperature. I need some help troubleshooting the fact that with both cryostat's the 1800 and the 1850 keep biteing into my chuck without advancing it. Please help...thanks Jennell ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From hhawkins <@t> utmb.edu Tue Apr 10 08:42:43 2007 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Tue Apr 10 08:42:54 2007 Subject: [Histonet] used microtome Message-ID: We are interested in purchasing a second used Leica 1512 microtome for our core histology lab, and need to specify the cost in our grant proposal. Can anyone help us find one and estimate the current market value? Thanks, Hal Hawkins UTMB, Galveston, and Shriners Burns Hospital From mber <@t> vt.edu Tue Apr 10 08:48:04 2007 From: mber <@t> vt.edu (mber@vt.edu) Date: Tue Apr 10 08:48:14 2007 Subject: [Histonet] Chicken gut processing times Message-ID: <1176212884.461b9594808f6@webmail.vt.edu> Hi all, Anyone have a good program for processing chicken gut (also bursa,spleen and thymus) from day of hatch and day 6 chicks? Thanks, Meg From POWELL_SA <@t> Mercer.edu Tue Apr 10 09:00:24 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Apr 10 09:01:40 2007 Subject: [Histonet] Bone saw In-Reply-To: <461B45D80200007700005337@gwmail.harthosp.org> Message-ID: <01MF93V8RGFW8X00QA@Macon2.Mercer.edu> Buehler Isomet Saw -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, April 10, 2007 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone saw We need a good saw for cutting thin slices of bone (4-5 mm.) to submit for microscopic evaluation following decalcification. Any suggestions? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Tue Apr 10 09:18:36 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Apr 10 09:19:01 2007 Subject: [Histonet] GI BX Message-ID: <461B566C020000AC000012DB@nodcmsngwia1.trinity-health.org> Histo Folks, This past week we have noticed that our GI Biopsies look like a train rails running through. Knives are sharp and techs that are sectioning have been in the field for 10 years plus. We do not process the GI's in a shorter processing, maybe I should. What are your thoughts. Also we are using PARAPLAST Plus Medium for both processing and embedding. Do most labs use different paraffin for embedding ? We have the Sakura VIP Tissue processors. I would appreciate any comments that you might share. If you have sunshine send it my way as we are to get 4 to 8 inches on new snow today and tomorrow. Marcia From yvan_lindekens <@t> yahoo.com Tue Apr 10 09:27:47 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Apr 10 09:27:55 2007 Subject: [Histonet] Old Leitz Kryomat freezing unit, looking for a manual... In-Reply-To: <01MF93V8RGFW8X00QA@Macon2.Mercer.edu> Message-ID: <733741.55455.qm@web30901.mail.mud.yahoo.com> Hi all, I recently aquired an old Kryomat freezing unit, (according to the label on it "type 25-062.111") from Leitz Wetzlar. The machine has a control panel/thermostatic unit type TKL-E/ made by Lauda (according to the label in it). I'm looking for a manual (or a xerox copy of it) for that machine. If you would have one and would sell it, I would be very gratefull! Thanks in advance! Yvan. ____________________________________________________________________________________ Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/newmail_tools.html From JHAPPEL <@t> PARTNERS.ORG Tue Apr 10 09:30:15 2007 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Tue Apr 10 09:31:01 2007 Subject: [Histonet] GI BX In-Reply-To: <461B566C020000AC000012DB@nodcmsngwia1.trinity-health.org> References: <461B566C020000AC000012DB@nodcmsngwia1.trinity-health.org> Message-ID: Marcia, What type of blades do you use, disposable or re-sharpen? James Happel James F. Happel, DLM (ASCP) HTL Technical Director of Surgical Pathology Massachusetts General Hospital Phone: 617.726.5153 * Fax: 617.726.6829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, April 10, 2007 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI BX Histo Folks, This past week we have noticed that our GI Biopsies look like a train rails running through. Knives are sharp and techs that are sectioning have been in the field for 10 years plus. We do not process the GI's in a shorter processing, maybe I should. What are your thoughts. Also we are using PARAPLAST Plus Medium for both processing and embedding. Do most labs use different paraffin for embedding ? We have the Sakura VIP Tissue processors. I would appreciate any comments that you might share. If you have sunshine send it my way as we are to get 4 to 8 inches on new snow today and tomorrow. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From lblazek <@t> digestivespecialists.com Tue Apr 10 09:36:24 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 10 09:36:19 2007 Subject: [Histonet] GI BX In-Reply-To: <461B566C020000AC000012DB@nodcmsngwia1.trinity-health.org> References: <461B566C020000AC000012DB@nodcmsngwia1.trinity-health.org> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F07ED92@bruexchange1.digestivespecialists.com> Marcia, I think you need to be more specific. Are the "train rails" showing up when you cut a ribbon? Is it only in GI biopsies? Is it a new and different batch of paraffin? Have you tried stirring the paraffin a bit before embedding? If it is during sectioning that these train rails are appearing then the problem is probably in your paraffin. Please don't send the snow my way. I'm sick of it. Since I'm in the basement and don't have any windows I don't know if there is any sun to send your way but I'll go check. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, April 10, 2007 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI BX Histo Folks, This past week we have noticed that our GI Biopsies look like a train rails running through. Knives are sharp and techs that are sectioning have been in the field for 10 years plus. We do not process the GI's in a shorter processing, maybe I should. What are your thoughts. Also we are using PARAPLAST Plus Medium for both processing and embedding. Do most labs use different paraffin for embedding ? We have the Sakura VIP Tissue processors. I would appreciate any comments that you might share. If you have sunshine send it my way as we are to get 4 to 8 inches on new snow today and tomorrow. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Don.Birgerson <@t> leica-microsystems.com Tue Apr 10 09:49:32 2007 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Tue Apr 10 09:50:45 2007 Subject: [Histonet] Old Leitz Kryomat freezing unit, looking for a manual... In-Reply-To: <733741.55455.qm@web30901.mail.mud.yahoo.com> Message-ID: Hi Yvan, I found a copy and will send it to you in a pdf format on separate E mail. If you have further questions, please phone me. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 yvan lindekens To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Old Leitz Kryomat freezing unit, looking for a 04/10/2007 09:27 manual... AM Hi all, I recently aquired an old Kryomat freezing unit, (according to the label on it "type 25-062.111") from Leitz Wetzlar. The machine has a control panel/thermostatic unit type TKL-E/ made by Lauda (according to the label in it). I'm looking for a manual (or a xerox copy of it) for that machine. If you would have one and would sell it, I would be very gratefull! Thanks in advance! Yvan. ____________________________________________________________________________________ Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/newmail_tools.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mber <@t> vt.edu Tue Apr 10 10:20:49 2007 From: mber <@t> vt.edu (mber@vt.edu) Date: Tue Apr 10 10:20:57 2007 Subject: [Histonet] Chicken Gut processing Message-ID: <1176218449.461bab51d759d@webmail.vt.edu> Hi All, I see that I forgot to include info. about myself. My name is Meg Berger and I am a histotech and work in the Animal and Poultry Sciences Department at VA Tech. If anyone could send me some information on processing times, I would really appreciate it ( I'm used to working with other animals, but not chickens). Thanks. Meg From Charles.Embrey <@t> carle.com Tue Apr 10 10:28:20 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Apr 10 10:28:31 2007 Subject: [Histonet] PA job description In-Reply-To: <20070409174519.0F4F630687B@mx5.carle.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4C4@EXCHANGEBE1.carle.com> I don't know of any PA's working for the VA system yet. It would be interesting to see what GS level they would place on the position and I would hate to be the one to petition the VA to create the position and fund it. Here is the official job description from the AAPA: At the direction and under the supervision of a Pathologist(s), a Pathologists' Assistant may perform the following tasks and assume the responsibility for duties including the following: Preparation, gross description and dissection of human tissue surgical specimens including: A. Assuring appropriate specimen accessioning. B. Obtaining clinical history, including scans, x-rays, laboratory data, etc. C. Describing gross anatomic features, dissecting surgical specimens, and preparing tissues for histologic processing. D. Obtaining biological specimens such as blood, tissue and toxicological material for studies such as flow cytometry, image analysis, immunohistochemistry etc., and performing special procedures including Faxitron imaging and tumor triage. E. Photographing all pertinent gross specimens and microscopic slides. F. Performing duties relating to the administrative maintenance of surgical pathology protocols, reports and data, including the filing of reports, protocols, photographic and microscopic slides; assuring the completion of specimen coding; and billing. G. Assuring proper maintenance of equipment, provision of adequate supplies, and cleanliness of the surgical pathology suite. H. Assisting in the organization and coordination of anatomic pathology conferences. Preparation of human postmortem examinations including: A. Ascertaining proper legal authorization for autopsy. B. Retrieving the patient's medical chart and other pertinent data for review with the attending pathologist(s). C. Conferring with the attending pathologist(s) to identify any special techniques and procedures to be utilized in the completion of the postmortem examination, (e.g. cultures; smears; histochemical, immunofluorescence, toxicological, viral, or electron microscopy studies etc.), and notifying all personnel directly involved. D. Notifying the physician in charge, the funeral home, and all other appropriate authorities prior to the beginning of the autopsy; and coordinating any requests for special specimen sampling (e.g. organ transplantation, research, etc.). E. Performing postmortem examinations which may include: external examination; in situ organ inspection; evisceration; dissection and dictation or recording of data such as organ weights, presence of body fluids etc., and gross anatomic findings. F. Selecting, preparing and submitting appropriate gross tissue sections for frozen section analysis as well as for light, electron and immunofluorescent microscopy. G. Obtaining biological specimens such as blood, tissue and toxicological material for studies including flow cytometry, image analysis, immunohistochemistry etc.; and performing special procedures such as coronary artery perfusion, central nervous system perfusion, enucleation, inner ear bone dissection, spinal cord removal, etc. H. Photographing the body, organs, microscopic slides and other pertinent materials. I. Gathering and organizing clinical information and data pertinent to the preparation of the preliminary summarization of the clinical history. J. Preparing the body for release (including indicating the presence of biohazards such as contagious disease, radiation implants, etc.), and releasing the body to the appropriate mortuary or funeral home representative. K. Performing duties related to administrative maintenance of anatomic pathology protocols; photographic and microscopic slides; and assuring the completion of coding. L. Assisting in the organization and coordination of anatomic pathology conferences. M. Assuring the proper maintenance of equipment, the provision of adequate supplies, and the cleanliness of the autopsy suite. Performing such administrative, budgetary, supervisory, teaching, and other duties as may be assigned Take care and good luck, Charles Embrey Jr. PA(ASCP) Carle Clinic, Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, April 09, 2007 1:42 PM To: 'R C'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 10 10:41:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 10 10:44:32 2007 Subject: [Histonet] PA job description In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE4C4@EXCHANGEBE1.carle.com> Message-ID: <386566.72623.qm@web61224.mail.yahoo.com> Charles: The AAPA, as you quote, has a pretty good idea of what a PA should do, and it is a comprehensive idea but it does not mean that in your specific case you can not limit those activities or duties, as I used to do for the PAs that worked under my supervision. What I always made sure was thay they had completed their accredited studies and had work experience. What you want them to do is one thing, and what they are able to do or are trained to do is another. Each lab should adapt to its particular needs. Ren? J. "Charles.Embrey" wrote: I don't know of any PA's working for the VA system yet. It would be interesting to see what GS level they would place on the position and I would hate to be the one to petition the VA to create the position and fund it. Here is the official job description from the AAPA: At the direction and under the supervision of a Pathologist(s), a Pathologists' Assistant may perform the following tasks and assume the responsibility for duties including the following: Preparation, gross description and dissection of human tissue surgical specimens including: A. Assuring appropriate specimen accessioning. B. Obtaining clinical history, including scans, x-rays, laboratory data, etc. C. Describing gross anatomic features, dissecting surgical specimens, and preparing tissues for histologic processing. D. Obtaining biological specimens such as blood, tissue and toxicological material for studies such as flow cytometry, image analysis, immunohistochemistry etc., and performing special procedures including Faxitron imaging and tumor triage. E. Photographing all pertinent gross specimens and microscopic slides. F. Performing duties relating to the administrative maintenance of surgical pathology protocols, reports and data, including the filing of reports, protocols, photographic and microscopic slides; assuring the completion of specimen coding; and billing. G. Assuring proper maintenance of equipment, provision of adequate supplies, and cleanliness of the surgical pathology suite. H. Assisting in the organization and coordination of anatomic pathology conferences. Preparation of human postmortem examinations including: A. Ascertaining proper legal authorization for autopsy. B. Retrieving the patient's medical chart and other pertinent data for review with the attending pathologist(s). C. Conferring with the attending pathologist(s) to identify any special techniques and procedures to be utilized in the completion of the postmortem examination, (e.g. cultures; smears; histochemical, immunofluorescence, toxicological, viral, or electron microscopy studies etc.), and notifying all personnel directly involved. D. Notifying the physician in charge, the funeral home, and all other appropriate authorities prior to the beginning of the autopsy; and coordinating any requests for special specimen sampling (e.g. organ transplantation, research, etc.). E. Performing postmortem examinations which may include: external examination; in situ organ inspection; evisceration; dissection and dictation or recording of data such as organ weights, presence of body fluids etc., and gross anatomic findings. F. Selecting, preparing and submitting appropriate gross tissue sections for frozen section analysis as well as for light, electron and immunofluorescent microscopy. G. Obtaining biological specimens such as blood, tissue and toxicological material for studies including flow cytometry, image analysis, immunohistochemistry etc.; and performing special procedures such as coronary artery perfusion, central nervous system perfusion, enucleation, inner ear bone dissection, spinal cord removal, etc. H. Photographing the body, organs, microscopic slides and other pertinent materials. I. Gathering and organizing clinical information and data pertinent to the preparation of the preliminary summarization of the clinical history. J. Preparing the body for release (including indicating the presence of biohazards such as contagious disease, radiation implants, etc.), and releasing the body to the appropriate mortuary or funeral home representative. K. Performing duties related to administrative maintenance of anatomic pathology protocols; photographic and microscopic slides; and assuring the completion of coding. L. Assisting in the organization and coordination of anatomic pathology conferences. M. Assuring the proper maintenance of equipment, the provision of adequate supplies, and the cleanliness of the autopsy suite. Performing such administrative, budgetary, supervisory, teaching, and other duties as may be assigned Take care and good luck, Charles Embrey Jr. PA(ASCP) Carle Clinic, Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, April 09, 2007 1:42 PM To: 'R C'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From Jason.Wiese <@t> va.gov Tue Apr 10 10:53:39 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Apr 10 10:53:55 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 In-Reply-To: <5q0p1d$2i7ag9@mtadal1.dal.net.va.gov> References: <2a926e3f0704090958p7f22457n47ef2e2f329e5ae1@mail.gmail.com> <5q0p1d$2i7ag9@mtadal1.dal.net.va.gov> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12919@VHAV20MSGA3.v20.med.va.gov> Douglas: In this case, I am the sole tech in pathology. I do everything from gross dictation to embedding, cutting, and staining. I am responsible for morgue operations and autopsies. I am responsible for all cytology and histology, including send outs, QA, QC, and all record keeping. I am an HT(ASCP), and my place is everywhere. I am finishing my ASCP certification for my PA as we speak. I am taking my PA BOR on Monday, and I am trying to break out of this mold I am in. As you say, "We all know that a PA's place is in the gross room anyway, leaving the histology matters to us." Not true with this PA, as my heart lies in histology. I am a born and bred HT, but I function as both PA and HT. I am simply trying to see if I could possibly find a PA job description and the series #, so I have a chance of squeezing a small raise out of my employer by showing I have a great amount of responsibility to get paid less then some support assistants here. Thanks to you, and anyone else who has tried to help!! Jason E. Wiese -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, April 09, 2007 11:42 AM To: 'R C'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Apr 10 12:09:56 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Apr 10 11:08:16 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12919@VHAV20MSGA3.v20.med.va.gov> Message-ID: Jason, I was mainly referring to the PA's with no histology training or experience. It sounds like you are on your way to showing your employers your worth. Sometimes a change is necessary to get proper compensation for your skills. I would say play the field and take the best offer back to your employer. I know... It's the government. :) Good luck. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@va.gov] Sent: Tuesday, April 10, 2007 10:54 AM To: Douglas D Deltour; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Douglas: In this case, I am the sole tech in pathology. I do everything from gross dictation to embedding, cutting, and staining. I am responsible for morgue operations and autopsies. I am responsible for all cytology and histology, including send outs, QA, QC, and all record keeping. I am an HT(ASCP), and my place is everywhere. I am finishing my ASCP certification for my PA as we speak. I am taking my PA BOR on Monday, and I am trying to break out of this mold I am in. As you say, "We all know that a PA's place is in the gross room anyway, leaving the histology matters to us." Not true with this PA, as my heart lies in histology. I am a born and bred HT, but I function as both PA and HT. I am simply trying to see if I could possibly find a PA job description and the series #, so I have a chance of squeezing a small raise out of my employer by showing I have a great amount of responsibility to get paid less then some support assistants here. Thanks to you, and anyone else who has tried to help!! Jason E. Wiese -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, April 09, 2007 11:42 AM To: 'R C'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Apr 10 11:08:36 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Apr 10 11:09:11 2007 Subject: [Histonet] Bone saw In-Reply-To: <01MF93V8RGFW8X00QA@Macon2.Mercer.edu> Message-ID: I'm looking for a band saw or reasonable facsimile to cut large frozen blocks of tissue for subsequent frozen section microtomy - any ideas? Jackie O' From Barbara_Lentz <@t> dahlchase.com Tue Apr 10 11:14:21 2007 From: Barbara_Lentz <@t> dahlchase.com (Barbara Lentz) Date: Tue Apr 10 11:16:01 2007 Subject: [Histonet] Rcycling 70/80% Alcohol Message-ID: Is there anybody out there recycling 70% or 80% alcohol? If so, which recycler are you using? Are you combining the lower % alcohols with 95/100%? If not, what is the percentage of the alcohol at the end of the recycle? Thanks for any info you can give. Barb From AJohnson <@t> aipathology.com Tue Apr 10 11:18:32 2007 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Tue Apr 10 11:18:40 2007 Subject: [Histonet] Processing time Message-ID: <4D6F15688B0DD34AA60F1C9A076BE57C029DC8@SERV001> Can anyone give me an estimate as to what the shortest possible processing time for biopsies can be using our Tissue Tek VIP processor. Thanks Amy Johnson From Charles.Embrey <@t> carle.com Tue Apr 10 11:28:51 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Apr 10 11:28:57 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12919@VHAV20MSGA3.v20.med.va.gov> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4C5@EXCHANGEBE1.carle.com> Good for you Jason. I myself am an Air Force trained and certified HT that got involved in grossing years ago and passed my AAPA Fellowship exam in 2000 and was grandfathered into the ASCP PA certification. Good luck on your exam. I don't know if the VA system will ever pay you what you are worth but from my experience the outside world highly prizes certified PAs with a strong histology background. Here I manage the histology lab as well as provide gross and autopsy coverage. If your boss ever wants to talk about PA salaries on the outside he can give me a call. I was involved teaching PA students at Rosalind Franklin in Chicago and know what my students were able to negotiate. Charles Embrey Jr. PA(ASCP) Carle Clinic, IL 217.379.0659 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Tuesday, April 10, 2007 10:54 AM To: Douglas D Deltour; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Douglas: In this case, I am the sole tech in pathology. I do everything from gross dictation to embedding, cutting, and staining. I am responsible for morgue operations and autopsies. I am responsible for all cytology and histology, including send outs, QA, QC, and all record keeping. I am an HT(ASCP), and my place is everywhere. I am finishing my ASCP certification for my PA as we speak. I am taking my PA BOR on Monday, and I am trying to break out of this mold I am in. As you say, "We all know that a PA's place is in the gross room anyway, leaving the histology matters to us." Not true with this PA, as my heart lies in histology. I am a born and bred HT, but I function as both PA and HT. I am simply trying to see if I could possibly find a PA job description and the series #, so I have a chance of squeezing a small raise out of my employer by showing I have a great amount of responsibility to get paid less then some support assistants here. Thanks to you, and anyone else who has tried to help!! Jason E. Wiese -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, April 09, 2007 11:42 AM To: 'R C'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue Apr 10 11:48:31 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Apr 10 11:48:43 2007 Subject: [Histonet] PA job description In-Reply-To: <386566.72623.qm@web61224.mail.yahoo.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4C6@EXCHANGEBE1.carle.com> I understand what you are saying but I was asked for a job description and I provided the one adopted by the American Association of Pathologists' Assistants (I guess they would have a pretty good idea of what a PA should do) and transferred over to the ASCP as their guidelines for PA activity. It is from that job description that the PA exam was written. I know each lab has the latitude to add or delete for the generic job description but a certified PA on the hunt for a job should be able to handle all the items on the AAPA checklist or risk unemployment. They don't have to master all requirements but should be familiar with them. Bear in mind here that I am talking about certified PAs or those wishing to challenge the certification exam. The job market is getting tighter out there and future employers want to be sure they are getting their money's worth. In Jason's case it sounds like they are getting a great deal more. Charles Embrey Jr. PA(ASCP) Carle Clinic IL -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, April 10, 2007 10:42 AM To: Charles.Embrey; Douglas D Deltour; R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PA job description Charles: The AAPA, as you quote, has a pretty good idea of what a PA should do, and it is a comprehensive idea but it does not mean that in your specific case you can not limit those activities or duties, as I used to do for the PAs that worked under my supervision. What I always made sure was thay they had completed their accredited studies and had work experience. What you want them to do is one thing, and what they are able to do or are trained to do is another. Each lab should adapt to its particular needs. Ren? J. "Charles.Embrey" wrote: I don't know of any PA's working for the VA system yet. It would be interesting to see what GS level they would place on the position and I would hate to be the one to petition the VA to create the position and fund it. Here is the official job description from the AAPA: At the direction and under the supervision of a Pathologist(s), a Pathologists' Assistant may perform the following tasks and assume the responsibility for duties including the following: Preparation, gross description and dissection of human tissue surgical specimens including: A. Assuring appropriate specimen accessioning. B. Obtaining clinical history, including scans, x-rays, laboratory data, etc. C. Describing gross anatomic features, dissecting surgical specimens, and preparing tissues for histologic processing. D. Obtaining biological specimens such as blood, tissue and toxicological material for studies such as flow cytometry, image analysis, immunohistochemistry etc., and performing special procedures including Faxitron imaging and tumor triage. E. Photographing all pertinent gross specimens and microscopic slides. F. Performing duties relating to the administrative maintenance of surgical pathology protocols, reports and data, including the filing of reports, protocols, photographic and microscopic slides; assuring the completion of specimen coding; and billing. G. Assuring proper maintenance of equipment, provision of adequate supplies, and cleanliness of the surgical pathology suite. H. Assisting in the organization and coordination of anatomic pathology conferences. Preparation of human postmortem examinations including: A. Ascertaining proper legal authorization for autopsy. B. Retrieving the patient's medical chart and other pertinent data for review with the attending pathologist(s). C. Conferring with the attending pathologist(s) to identify any special techniques and procedures to be utilized in the completion of the postmortem examination, (e.g. cultures; smears; histochemical, immunofluorescence, toxicological, viral, or electron microscopy studies etc.), and notifying all personnel directly involved. D. Notifying the physician in charge, the funeral home, and all other appropriate authorities prior to the beginning of the autopsy; and coordinating any requests for special specimen sampling (e.g. organ transplantation, research, etc.). E. Performing postmortem examinations which may include: external examination; in situ organ inspection; evisceration; dissection and dictation or recording of data such as organ weights, presence of body fluids etc., and gross anatomic findings. F. Selecting, preparing and submitting appropriate gross tissue sections for frozen section analysis as well as for light, electron and immunofluorescent microscopy. G. Obtaining biological specimens such as blood, tissue and toxicological material for studies including flow cytometry, image analysis, immunohistochemistry etc.; and performing special procedures such as coronary artery perfusion, central nervous system perfusion, enucleation, inner ear bone dissection, spinal cord removal, etc. H. Photographing the body, organs, microscopic slides and other pertinent materials. I. Gathering and organizing clinical information and data pertinent to the preparation of the preliminary summarization of the clinical history. J. Preparing the body for release (including indicating the presence of biohazards such as contagious disease, radiation implants, etc.), and releasing the body to the appropriate mortuary or funeral home representative. K. Performing duties related to administrative maintenance of anatomic pathology protocols; photographic and microscopic slides; and assuring the completion of coding. L. Assisting in the organization and coordination of anatomic pathology conferences. M. Assuring the proper maintenance of equipment, the provision of adequate supplies, and the cleanliness of the autopsy suite. Performing such administrative, budgetary, supervisory, teaching, and other duties as may be assigned Take care and good luck, Charles Embrey Jr. PA(ASCP) Carle Clinic, Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, April 09, 2007 1:42 PM To: 'R C'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Hey Rueben let me guess, you are a PA? I don't think Jason would be supervising a PA. We all know that a PA's place is in the gross room anyway, leaving the histology matters to us. I am sure that Jason's pathologist doesn't have time to write it so he left it to him. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, April 09, 2007 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 8 Re: Pathogists Assistant job descriptions request: Wouldn't your staff pathologists serve as a great source for their needs and requirements in a Pathologist Assistant? Would you serve as the PA supervisor? Rueben Carter On 4/5/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD's for PA's (Wiese, Jason VHAROS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Apr 2007 09:58:37 -0700 > From: "Wiese, Jason VHAROS" > Subject: [Histonet] PD's for PA's > To: "Histonet" > Message-ID: > <70EEF3D43B3C164C94037D811B2BE193E12905@VHAV20MSGA3.v20.med.va.gov> > Content-Type: text/plain; charset="us-ascii" > > > > Hello All! > > > > I hope this message finds you all healthy and happy... > > > > I am hoping someone in histoland can help me. I need to come up with > some position descriptions for Pathologist Assistants. I would really > like to find a PA that works for the Department of Veteran Affairs, but > anything will help. I need to know the series and grade for a PA in the > VA system. > > > > Thanks you in advance! > > > > Jason > > > > > > > > Jason E. Wiese, BS, HT(ASCP) > > VAROS Histology/Cytology > > 913 NW Garden Valley Blvd. > > Roseburg, OR 97470 > > (541)440-1000 ext. 44751 > > jason.wiese@med.va.gov > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 41, Issue 8 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From rchiovetti <@t> yahoo.com Tue Apr 10 11:54:24 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Tue Apr 10 11:54:31 2007 Subject: [Histonet] Bone saw Message-ID: <966590.96683.qm@web58914.mail.re1.yahoo.com> You might try Mopec. They have some devices that are designed to hold and cut thin slices of bone, as well as Gigli wire saws (OK, OK, no snide remarks about the JLo movie...) For example: Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Jackie M O'Connor To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 10, 2007 9:08:36 AM Subject: RE: [Histonet] Bone saw I'm looking for a band saw or reasonable facsimile to cut large frozen blocks of tissue for subsequent frozen section microtomy - any ideas? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. http://tools.search.yahoo.com/toolbar/features/mail/ From gentras <@t> vetmed.auburn.edu Tue Apr 10 12:16:48 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Tue Apr 10 12:16:59 2007 Subject: [Histonet] IHC Message-ID: <461BC680.1050308@vetmed.auburn.edu> hello, is anyone doing any phage IHC staining, preferably on frozen sections? One of our researchers is trying to come up with a suitable staining protocol and I decided to inquire on blocking agents used including recommended percentages. Thank you. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From ynwang <@t> u.washington.edu Tue Apr 10 12:36:04 2007 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue Apr 10 12:37:24 2007 Subject: [Histonet] Artifact in NADH staining Message-ID: Hello all, I have a question regarding some peculiar staining I am getting. Sorry for the long post. Goal: The goal is to identify lesion size by looking at the area of dead cells using enzyme histochemical analysis. Experimental details: Fresh bovine liver (ex vivo) is treated with high intensity focused ultrasound, to form distinct lesions. Immediately following treatment the tissue is immediately frozen (embedded in OCT using cooled isopentane). 6-8 um sections are taken and incubated with NADH + nitroblue tetrazolium in buffer (Ringers and PBS) and then rinsed in DI water before being mounted in aqueous mounting medium (as described by Neumann RA et al. 1991 "Enzyme histochemical analysis of cell viability after argon laser-induced coagulationn necrosis of skin"). Negative control is untreated liver, positive control is cooked liver. Results and problem: Control tissue and untreated tissue stains very nicely. We see a purple stain localized to the cytoplasm of the cells as expected from the formazan produced from the reaction. The surrounding ECM is not stained, nor are the nuclei. The positive control and most regions of the treated tissue also give expected results- the cells are surrounding areas are unstained. However, in some areas of the treated tissue (in distinct geometries) we are seeing this deep blue-black stain around the cells (the cells are not stained). I was wondering what this blue-black stain is. Does anyone have any ideas? I have seem some reference to a blue-black crystalline precipitate (Novikoff 1959 and Fallon 1974) that form on lipide droplets but haven't been able to find any further explanation. I don't even know if this is even what I am seeing as I do not see 'crystals'. I have just sent a picture to be posted on www.histonet.org I believe it will take 24 hrs to get it posted on the site. Name is NADH staining artifact. We are stumped. Any insight would be greatly appreciated. Thank you Yak-Nam From lisalocum <@t> yahoo.co.uk Tue Apr 10 12:51:10 2007 From: lisalocum <@t> yahoo.co.uk (lisa mccluskey) Date: Tue Apr 10 12:51:19 2007 Subject: [Histonet] fluoro-jade B and PFA perfused tissue Message-ID: <229442.76829.qm@web25010.mail.ukl.yahoo.com> I have been using fluoro-jade B on two types of brain tissue- fresh frozen and that perfused with 4% PFA, post-fixed with PFA for 24h, incubated in 30% sucrose for 72h then stored at -20C before being cut on a cryostat. The stain works nicely on fresh frozen tissue, but in PFA fixed tissue only gives dim staining, not easily differentiated from control staining on the contra-lateral side. I have tried omitting the pot perm stage to try and increase staining (including background) but this doesn't make much difference. Has anyone else had this problem and does anyone have any ideas how to fix it, thanks, Lisa --------------------------------- Yahoo! Answers - Got a question? Someone out there knows the answer. Tryit now. From gcallis <@t> montana.edu Tue Apr 10 13:38:10 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 10 13:38:11 2007 Subject: recommendations for Re: [Histonet] Bone saw In-Reply-To: <461B45D80200007700005337@gwmail.harthosp.org> References: <461B45D80200007700005337@gwmail.harthosp.org> Message-ID: <6.0.0.22.1.20070410121426.01b6fad0@gemini.msu.montana.edu> Dear Richard, MarMed Bone saw is superb. Will not cut you if your fingers are in the way and touch the blade/saw, plus it shields you from aerosol, debris, etc and not terribly pricey. Countertop setup, so it is NOT a gigantic monster to deal with and can be cleaned easily. I saw one at IMEB exhibit at Region 3 meeting and in a laboratory setting. I found it here (a horrible website!) http://www.diruscioassociates.com under histology items. You would need to call them for particulars, couldn't find a photo on their website. A Histonet reply years back recommended a wild game processing Cabela's commercial butcher band saw, $599.99, Item # Item:IH-516404 from www.cabelas.com. It has washable parts although not shielded. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Tue Apr 10 13:49:47 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 10 13:49:48 2007 Subject: [Histonet] Bone saw In-Reply-To: References: <01MF93V8RGFW8X00QA@Macon2.Mercer.edu> Message-ID: <6.0.0.22.1.20070410124302.01b69b88@gemini.msu.montana.edu> Jackie, That Cabelas commercial butcher saw would probably do the job for you for really large bones but remember that the actual passing of a saw blade through a frozen bone will thaw the bone at the sawing site, simply due to the heat created by the friction of the blade passing through the hard bone. Is there no way the bone can be sawn BEFORE freezing, unless you do not care about some bone loss. We always cut our samples before doing any freezing. And the smaller the bone you need for undecalcified bone sections means there will probably be more thawing and then refreezing on the bone surface. The MarMed saw would probably do the job too, but the Buehler Isomet is low speed but requires a water cooling bath during the sawing process. The Isomet is really best for small bones and not large bones as the cutoff blades are not very large diameter, meaning much shallower cuts overall. I hae seen hobby band saws used for cutting bone samples even when embedded in PMMA and these saws sit on a countertop. At 10:08 AM 4/10/2007, you wrote: >I'm looking for a band saw or reasonable facsimile to cut large frozen >blocks of tissue for subsequent frozen section microtomy - any ideas? > >Jackie O' >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From TJJ <@t> Stowers-Institute.org Tue Apr 10 14:36:46 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Apr 10 14:37:13 2007 Subject: [Histonet] Karyotyping mouse cells Message-ID: Does anybody know a place we can send mouse cells for karyotyping/chromosome analysis? I've contacted places that do human cells, but not mouse. Thanks for your help, as always! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From rjbuesa <@t> yahoo.com Tue Apr 10 14:47:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 10 14:48:02 2007 Subject: [Histonet] Rcycling 70/80% Alcohol In-Reply-To: Message-ID: <231069.96865.qm@web61214.mail.yahoo.com> It is not economic to recycle 70E or 80E It requires energy for longer time than recycling used 95E or "used" 100E Unless you are paying a premium for absolute ethanol, recycling it is not worth the cost. Ren? J. Barbara Lentz wrote: Is there anybody out there recycling 70% or 80% alcohol? If so, which recycler are you using? Are you combining the lower % alcohols with 95/100%? If not, what is the percentage of the alcohol at the end of the recycle? Thanks for any info you can give. Barb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From rjbuesa <@t> yahoo.com Tue Apr 10 14:51:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 10 14:51:23 2007 Subject: [Histonet] Processing time In-Reply-To: <4D6F15688B0DD34AA60F1C9A076BE57C029DC8@SERV001> Message-ID: <540129.6389.qm@web61212.mail.yahoo.com> I used to have a 1h18min protocol for biopsies fixed in alcoholic fixative, and another of 2h40min for regular small biopsies fixed in formalin. Ren? J. Amy Johnson wrote: Can anyone give me an estimate as to what the shortest possible processing time for biopsies can be using our Tissue Tek VIP processor. Thanks Amy Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From cforster <@t> umn.edu Tue Apr 10 15:10:52 2007 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Apr 10 15:10:59 2007 Subject: [Histonet] cutting frozen sections of mouse eyeball Message-ID: <461BEF4C.7000300@umn.edu> I have been asked about cutting frozen sections on mouse eyeball.....anyone that has done this, please share your experience. Colleen Forster U of MN From JHAPPEL <@t> PARTNERS.ORG Tue Apr 10 15:29:49 2007 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Tue Apr 10 15:30:01 2007 Subject: [Histonet] When staining IHC's, are labs putting control tissue on every slide? In-Reply-To: <7F2A2AE306CE254DB7279E86A51A7406125E33@CMROCEX01.cellmarque.local> References: <7F2A2AE306CE254DB7279E86A51A7406125E33@CMROCEX01.cellmarque.local> Message-ID: Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From mcauliff <@t> umdnj.edu Tue Apr 10 15:53:58 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Apr 10 15:52:52 2007 Subject: [Histonet] bouins fixed mouse tissue In-Reply-To: References: Message-ID: <461BF966.2030806@umdnj.edu> Hi Helen: Bouin's fixed material is usually very easy to cut. Depending on how large your specimens are 15 min per station may not be enough. Geoff Helen E Johnson wrote: >Dear Histonetters, > Has anyone experienced chatter artifact while sectioning Bouins >fixed mouse tissue? The tissue is fixed in Bouins fixative for 24 hrs. and >transferred to 70% EtOH for rinsing. The tissues are processed 15 min. at >each solution station. >Helen Johnson (hej01@health.state.ny.us.) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From mcauliff <@t> umdnj.edu Tue Apr 10 15:56:21 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Apr 10 15:55:21 2007 Subject: [Histonet] bouins fixed mouse tissue In-Reply-To: <103106.48881.qm@web31315.mail.mud.yahoo.com> References: <103106.48881.qm@web31315.mail.mud.yahoo.com> Message-ID: <461BF9F5.5060909@umdnj.edu> Shortening the fixation in Bouin's will NOT solve the problem and it may make it worse. Tissue can be left in Bouin's for a week or more without harm. Geoff Akemi Allison-Tacha wrote: >Helen, >As you probably know, mouse tissue is much more >delicate than human tissue. If you have control over >the fixation process, I would cut down the initial >fixation time in Bouin's solution. Perhaps, try 6-12 >hours. > >For the tissue that you have already processed, soak >in a tween 80 and water mixture of 1:10 for a minimum >of 10 minutes, followed by a water wash to remove >tween, then soak on a ice tray. This works >beautifully for over-processed tissue, skin, uterus, >ect. > >PS: Tween 20 can be substituted or a similar >surfactant. > >Good Luck, >Akemi Allison-Tacha BS, HT (ASCP) HTL >President >Phoenix Lab Consulting & Staffing >Specializing in Histology, SS, IHC, & Microarray >Madison, WI >Tele: (925) 788-0900 >E-Mail: akemiat3377@yahoo.com > >--- Andrea Grantham wrote: > > > >>helen, >>What kind of tissue are you trying to cut? >>I frequently get mouse ovaries fixed for 24 hrs in >>Bouins and transfered to >>70% ETOH. They usually cut fine but in your case my >>suggestion is SOAK, >>SOAK, SOAK in icy water. >>Not long ago someone here suggested putting a little >>glycerin in the >>soaking water - you can find the actual posts on the >>archives - but I tried >>it and was delighted with the results. >> >>Andi Grantham >> >> >>At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote: >> >> >> >>>Dear Histonetters, >>> Has anyone experienced chatter artifact >>> >>> >>while sectioning Bouins >> >> >>>fixed mouse tissue? The tissue is fixed in Bouins >>> >>> >>fixative for 24 hrs. and >> >> >>>transferred to 70% EtOH for rinsing. The tissues >>> >>> >>are processed 15 min. at >> >> >>>each solution station. >>>Helen Johnson (hej01@health.state.ny.us.) >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>> >>> >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >..................................................................... > > >>: Andrea Grantham, HT(ASCP) Dept. of Cell >>Biology & Anatomy : >>: Sr. Research Specialist University of >>Arizona : >>: (office: AHSC 4212) P.O. Box 245044 >> : >>: (voice: 520-626-4415) Tucson, AZ >>85724-5044 USA : >>: (FAX: 520-626-2097) (email: >>algranth@u.arizona.edu) : >> >> >> >:...................................................................: > > >> >>http://www.cba.arizona.edu/histology-lab.html >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >> >> >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From akemiat3377 <@t> yahoo.com Tue Apr 10 16:03:19 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Apr 10 16:03:26 2007 Subject: [Histonet] McMannas PAS Light Green In-Reply-To: <012101c77afe$12fcaea0$11614542@yourxhtr8hvc4p> Message-ID: <670654.89759.qm@web31314.mail.mud.yahoo.com> Just a heads-up on Light Green Dye.... There has been a problem with Light Green Dye for the past several years. It ain't what it used to be!! Atleast, that was some inside info I was given from some reputable sources from the Biological Stains Commission. As a solution, I developed a Fast-Light Green Counterstain for Biocare's Masterpiece Line of Special Stains. My formulation is proprietary, but you can purchase it from Biocare. I used it for the PAS/Lt Green for Fungus Stain and it looked beautiful. Don't worry--I don't getting any $$ from them since leaving. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Joe Nocito wrote: > Matt, > we perform a PAS/Fungus on all toenails we gross > (hence Joe the Toe). The > doctors I work for like out light green strong. I > can't count how many times > they kicked back trays of slides that weren't > stained dark enough. We have a > TissueTek stain line that we use for our special > stains. We have an extra > container filled with 95% ETOH we use to rinse off > the Fast Green. Every > time we did a quick rinse in water, the green always > washed out. Personally, > the magenta color with the dark green just curls my > toes up. I'm thinking of > painting my living room with those colors. > > JTT > > ----- Original Message ----- > From: > To: > Sent: Monday, April 09, 2007 8:50 AM > Subject: [Histonet] McMannas PAS Light Green > > > > Morning Histonetters, > > > > I am in need of everyones help. I am doing a > McMannas PAS with light green > > on a fungus but am un-sure how much the light > green it to show. Every time > > I have done this stain so far the light green has > been extremly faint. > > > > I have been using a fungus in lung control. > > > > I have also been unable to find a photo of what > this stain should look > > like. > > > > Thanks for all of your Help. > > Matt Lunetta > > Longmont United Hospital > > > ________________________________________________________________________ > > AOL now offers free email to everyone. Find out > more about what's free > > from AOL at AOL.com. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From katri <@t> cogeco.ca Tue Apr 10 21:23:14 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Tue Apr 10 21:22:58 2007 Subject: [Histonet] When staining IHC's, are labs putting control tissue on every slide? References: <7F2A2AE306CE254DB7279E86A51A7406125E33@CMROCEX01.cellmarque.local> Message-ID: <003101c77be0$5cad23d0$6a9a9618@Katri> We service over 20 pathologists. Some of them want the control tissue on every slide (only one negative test and control is run per case with the harshest retrieval), some are happy with one control tissue for each antibody per run. We go through an awful lot of controls... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Happel, James F." To: Sent: Tuesday, April 10, 2007 4:29 PM Subject: [Histonet] When staining IHC's,are labs putting control tissue on every slide? Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.Tyler <@t> uct.ac.za Wed Apr 11 00:21:03 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Wed Apr 11 00:21:25 2007 Subject: [Histonet] Mouse path Message-ID: <461C8C5F.A78E.0090.0@uct.ac.za> Hi All Histonetters A REALLY BIG THANK YOU to the great response. I will look into all the information and find what is needed for my use. Marilyn From yelkaco <@t> ticon.net Wed Apr 11 00:42:08 2007 From: yelkaco <@t> ticon.net (S. Coakley) Date: Wed Apr 11 00:33:05 2007 Subject: [Histonet] As needed HT Message-ID: <000d01c77bfc$25ae3f60$5ba05f45@yelkaco> I'm looking for as needed HT work in the So WI/ No Ill area. From Djemge <@t> aol.com Wed Apr 11 01:50:20 2007 From: Djemge <@t> aol.com (Djemge@aol.com) Date: Wed Apr 11 01:50:33 2007 Subject: [Histonet] Re: McManus ("McMannas") PAS Light Green Message-ID: Matt, often a faint light green counterstain for the PAS or GMS stains is caused by not rinsing long enough after the periodic acid. Also the light green often needs a fairly rapid dehydration before clearing and coverslipping. Donna Emge, HT(ASCP) Northwestern University 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 _d-emge@northwestern.edu_ (mailto:d-emge@northwestern.edu) ************************************** See what's free at http://www.aol.com. From Djemge <@t> aol.com Wed Apr 11 02:11:56 2007 From: Djemge <@t> aol.com (Djemge@aol.com) Date: Wed Apr 11 02:12:05 2007 Subject: [Histonet] Re: Bouins fixed mouse tissue Message-ID: Helen, I agree with Andi. My lab mainly uses Bouin's for adult mouse testes with excellent results. We fix 8 to 24 hours in Bouin's, rinse several times throughout the day with water, then 2 days in 50% ETOH. Then the processing schedule is 30 minutes each station. Sometimes I go up to 1 hr each station if I have a lot of other tissue that needs longer processing. Both schedules have produced excellent results in our lab and the faced blocks need only a few minutes of wet ice soaking for beautiful sections. I still have not gotten very good results with formalin fixed adult mouse testes. The formalin blocks look good, cut good, but morphologically look bad. So I stick with the Bouins. Donna Emge, HT(ASCP) Northwestern University 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 _d-emge@northwestern.edu_ (mailto:d-emge@northwestern.edu) ************************************** See what's free at http://www.aol.com. From GDawson <@t> dynacaremilwaukee.com Wed Apr 11 07:20:43 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Apr 11 07:20:50 2007 Subject: [Histonet] When staining IHC's, are labs putting control tissue on every slide? In-Reply-To: <003101c77be0$5cad23d0$6a9a9618@Katri> Message-ID: I put a positive control on every slide with one negative run per case with the harshest (or most likely to cause staining retrieval/detection used on the case). We go through obnoxious amounts of controls but I've found we have almost no repeats as it is hard for a pathologist to argue that an IHC did not work when the patient tissue is on the same slide as the positive control. Also, just because the control for, say CD20, worked in position one, doesn't mean that the CD20 in position 32 didn't experience a machine malfunction or that it got the right reagents/antibody (especially when the workload calls for multiple vials of each). Having the control on the same slide as the patient tissue also impresses CAP inpectors as it is, essentially, an on board validation for a working antibody. Glen Dawson BS, HT, & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Katri Tuomala Sent: Tuesday, April 10, 2007 9:23 PM To: Happel, James F.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] When staining IHC's,are labs putting control tissue on every slide? We service over 20 pathologists. Some of them want the control tissue on every slide (only one negative test and control is run per case with the harshest retrieval), some are happy with one control tissue for each antibody per run. We go through an awful lot of controls... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Happel, James F." To: Sent: Tuesday, April 10, 2007 4:29 PM Subject: [Histonet] When staining IHC's,are labs putting control tissue on every slide? Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tiffany.Price <@t> thomaswv.org Wed Apr 11 09:06:02 2007 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Wed Apr 11 09:08:36 2007 Subject: [Histonet] validation of IHC slides for Benchmark Stainer Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C3221C@thm-mail.thomaswv.org> Could someone tell me if I need to keep all of the slides that I stained to optimize my IHC protocols, the slide for the final protocol I keep, or is a printed log signed off by the Pathologist sufficient for documentation? Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From Robinsoc <@t> mercyhealth.com Wed Apr 11 09:25:04 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Wed Apr 11 09:25:26 2007 Subject: [Histonet] validation of IHC slides for Benchmark Stainer In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49103C3221C@thm-mail.thomaswv.org> References: <7B5F5D9A00739F43A1819403EC7CF49103C3221C@thm-mail.thomaswv.org> Message-ID: <461CA970020000AF000004C4@nodcmsngwia1.trinity-health.org> We kept all of our slides from our recent XT install in November and were just CAP inspected yesterday. I did document on a spreadsheet the final protocols. As we passed our inspection with no hits I think I can discard the slides now and just keep paperwork. Cindi Robinson, HT (ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Price, Tiffany" 4/11/2007 9:06 AM >>> Could someone tell me if I need to keep all of the slides that I stained to optimize my IHC protocols, the slide for the final protocol I keep, or is a printed log signed off by the Pathologist sufficient for documentation? Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbott.com Wed Apr 11 09:51:37 2007 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Wed Apr 11 09:51:52 2007 Subject: [Histonet] Liver sections cracking in drying stage..Help In-Reply-To: <5p35cf$3va6qg@viper.abbott.com> Message-ID: Hi All, I hope someone can help with this problem. I am working on a toxicology study that requires sections of Heart, liver and Lung from rats on study . These studies we want a quick turn around so we get the tissues from necropsy in the afternoon, livers are scored at necropsy and put into formalin jars. I gross the samples Liver (left, midial, caudal lobes into one cassette) the next morning done by 11am (liver is still pink in the middle). The samples are put on the processor (11 hour processor run) that night with formalin in the first station so fixation after grossing is 10 hours. The next day I embed and section the samples and dry the sections overnight. Slides are stained and given to the pathologist that day. My problem is the liver samples all other blocks are fine, the liver blocks section fine but on drying either in a rotating drying oven vertically or on the bench at room temp overnight some NOT all of the liver samples have lots of cracks and some fall off. It very much looks like areas of water as it dried causes these cracks. Anyone have ideas to remedy this problem..?? They want to keep this turn around time if possible and liver is a key read out... Any ideas... Thanks Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From rjbuesa <@t> yahoo.com Wed Apr 11 10:14:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 11 10:14:09 2007 Subject: [Histonet] Liver sections cracking in drying stage..Help In-Reply-To: Message-ID: <270966.77723.qm@web61215.mail.yahoo.com> Jamie: If I understood you well you have a 11 hours protocol of which 10 hours are fixation? If this is true I think your processing steps (other than fixation) are too fast even if rat tissues are leaner than human. If in spite of 10 hours fixation you still have reeddish centers in the liver slices: how thick they are? How many they are per cassette? You should "open" the liver samples (the ones going into the first fixative after necropsy) to assure a good fixation. I think you should prepare the cassettes immediately after necropsy and set the slices to fix. If you cassette after fixation and place the cassettes in the tissue processor you could embed the next and section the morning which will reduce the TAT. I did not understand the "drying overnight" step you describe. Why so long? I think you have a mixed problem between incomplete initial fixation and too fast tissue processing (1 of 11 hours protocol). Ren? J. Jamie E Erickson wrote: Hi All, I hope someone can help with this problem. I am working on a toxicology study that requires sections of Heart, liver and Lung from rats on study . These studies we want a quick turn around so we get the tissues from necropsy in the afternoon, livers are scored at necropsy and put into formalin jars. I gross the samples Liver (left, midial, caudal lobes into one cassette) the next morning done by 11am (liver is still pink in the middle). The samples are put on the processor (11 hour processor run) that night with formalin in the first station so fixation after grossing is 10 hours. The next day I embed and section the samples and dry the sections overnight. Slides are stained and given to the pathologist that day. My problem is the liver samples all other blocks are fine, the liver blocks section fine but on drying either in a rotating drying oven vertically or on the bench at room temp overnight some NOT all of the liver samples have lots of cracks and some fall off. It very much looks like areas of water as it dried causes these cracks. Anyone have ideas to remedy this problem..?? They want to keep this turn around time if possible and liver is a key read out... Any ideas... Thanks Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From Eric.C.Kellar <@t> questdiagnostics.com Wed Apr 11 10:17:38 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Wed Apr 11 10:17:59 2007 Subject: [Histonet] validation of IHC slides for Benchmark Stainer Message-ID: <6843061CE6B98E4B96590D4F299618F801583C17@qdcws0117.us.qdx.com> It's best to keep the original slides from your validation indefinitely along with the original signed validation procedure documentation. I keep mine in a binder with original Benchmark signed validation document and slide sleeve holding original slides (some remounted after being broken since 2003) used to validate each antibody. If we are to keep blocks and slides for 10 years, why would the original documentation of antibody validation and the slides be any different? Keep them for future reference. Future CAP inspectors will be glad you did, and it will save you and your colleagues from revalidating those antibodies again. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Robinson Sent: Wednesday, April 11, 2007 10:25 AM To: histonet@lists.utsouthwestern.edu; Tiffany Price Subject: Re: [Histonet] validation of IHC slides for Benchmark Stainer We kept all of our slides from our recent XT install in November and were just CAP inspected yesterday. I did document on a spreadsheet the final protocols. As we passed our inspection with no hits I think I can discard the slides now and just keep paperwork. Cindi Robinson, HT (ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Price, Tiffany" 4/11/2007 9:06 AM >>> Could someone tell me if I need to keep all of the slides that I stained to optimize my IHC protocols, the slide for the final protocol I keep, or is a printed log signed off by the Pathologist sufficient for documentation? Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Apr 11 10:44:18 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Apr 11 10:44:12 2007 Subject: [Histonet] Liver sections cracking in drying stage..Help In-Reply-To: References: Message-ID: <461D0252.9020300@umdnj.edu> Hi Jamie: I think I agree with Rene, not enough fixation. I suggest changing fixative to a formalin-alcohol-acetic mixture. It will fix much faster and give excellent preservation of glycogen. It frightens me to think that turn around time is more important than good results. Geoff Jamie E Erickson wrote: >Hi All, > I hope someone can help with this problem. I am working on a >toxicology study that requires sections of Heart, liver and Lung from rats >on study . These studies we want a quick turn around so we get the tissues >from necropsy in the afternoon, livers are scored at necropsy and put >into formalin jars. I gross the samples Liver (left, midial, caudal lobes >into one cassette) the next morning done by 11am (liver is still pink in >the middle). The samples are put on the processor (11 hour processor run) >that night with formalin in the first station so fixation after grossing >is 10 hours. The next day I embed and section the samples and dry the >sections overnight. Slides are stained and given to the pathologist that >day. > >My problem is the liver samples all other blocks are fine, the liver >blocks section fine but on drying either in a rotating drying oven >vertically or on the bench at room temp overnight some NOT all of the >liver samples have lots of cracks and some fall off. It very much looks >like areas of water as it dried causes these cracks. Anyone have ideas to >remedy this problem..?? They want to keep this turn around time if >possible and liver is a key read out... Any ideas... > >Thanks > >Jamie >_______________________________ >Jamie Erickson >Sr. Research Associate >Department: DSMP >Abbott Bioresearch Center >100 Research Drive >Worcester, MA 01605-4341 >508-688-3134 >FAX: 508-793-4895 >e-mail: jamie.erickson@abbott.com > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From yvan_lindekens <@t> yahoo.com Wed Apr 11 10:50:03 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Wed Apr 11 10:50:15 2007 Subject: [Histonet] staining baskets for Medite COT 20 Message-ID: <201795.65492.qm@web30910.mail.mud.yahoo.com> Hi Histonetters, I'm looking (preferably in Europe) for some staining baskets for a liniar slide stainer Medite COT 20. Of course I'm willing to pay a fair price, but I need them to make ...well... lots of free slides for some high schools in Africa (Namibia), so cost is a concern and the prices asked by Medite are outrageous. Perhaps you have some gathering dust in the basement? Thanks in advance, Yvan. ____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091 From rsrichmond <@t> aol.com Wed Apr 11 11:02:06 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Apr 11 11:02:30 2007 Subject: [Histonet] Re: Bone saw In-Reply-To: <200704111145.158461d028c38@rly-ya04.mx.aol.com> References: <200704111145.158461d028c38@rly-ya04.mx.aol.com> Message-ID: <8C94A69E2E0FF31-C34-38AE@webmail-mf05.sysops.aol.com> Most surgical pathologists cut bone with an electric Stryker oscillating saw, since their autopsy service usually has one. These really aren't suitable for surgical pathology, since it's difficult to hold the specimen down, particularly if you don't have a bench vise. I've always relied on hand-held saws. The old Satterlee amputation saw, of Civil War (1861-65) vintage, is the usual saw available. (I've twice seen these in Civil War re-enactor hospitals, complete with chrome plating, right like they come from whatever Lipshaw is called this week.) Many small surgical pathology services have no saw of any kind. When I work one of these services, I go to a hardware store and spend five dollars on a hacksaw, and leave it behind when I go. An elegant variation on the hacksaw is the "Sawbones", which combines a sturdy clamp with two parallel long hacksaw blades that cut a slab of a femoral head a few mm thick. It's rather overpriced at about $500, prohibitively expensive for something the pathologist is actually going to have his hands on. The bone pathologist at Johns Hopkins recommends a tabletop scroll saw. These cost about $200. They're supposed to be quite safe, but they have too large a footprint for the usual cramped surgical pathology lab. (Meanwhile, down the hall, the radiology department just bought a 64-slice CT scanner, their toy of the year. The red-haired stepchild....) Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 11 11:32:44 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 11 11:32:53 2007 Subject: [Histonet] Re: Bone saw Message-ID: <407F05A128805F4C879A33DBA32E618E20C2BC@TRFT-EX01.xRothGen.nhs.uk> "(Meanwhile, down the hall, the radiology department just bought a 64-slice CT scanner, their toy of the year. The red-haired stepchild....)" It's one of the strangest facts of life that radiologists can get what they want, just to see shadows. Millions - no problem, but a few measly thousand spent in pathology, where the real diagnoses are made - sharp intake of breath with a "not so sure about that". Terry PS The recurring problem of sawing bones drives me nuts. The sawing bit is easy, you just need a saw, it's the holding of the bone that's the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: 11 April 2007 17:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Bone saw Most surgical pathologists cut bone with an electric Stryker oscillating saw, since their autopsy service usually has one. These really aren't suitable for surgical pathology, since it's difficult to hold the specimen down, particularly if you don't have a bench vise. I've always relied on hand-held saws. The old Satterlee amputation saw, of Civil War (1861-65) vintage, is the usual saw available. (I've twice seen these in Civil War re-enactor hospitals, complete with chrome plating, right like they come from whatever Lipshaw is called this week.) Many small surgical pathology services have no saw of any kind. When I work one of these services, I go to a hardware store and spend five dollars on a hacksaw, and leave it behind when I go. An elegant variation on the hacksaw is the "Sawbones", which combines a sturdy clamp with two parallel long hacksaw blades that cut a slab of a femoral head a few mm thick. It's rather overpriced at about $500, prohibitively expensive for something the pathologist is actually going to have his hands on. The bone pathologist at Johns Hopkins recommends a tabletop scroll saw. These cost about $200. They're supposed to be quite safe, but they have too large a footprint for the usual cramped surgical pathology lab. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Apr 11 11:36:35 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Apr 11 11:37:02 2007 Subject: [Histonet] Re: Bone saw In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2BC@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3B88@sjhaexc02.sjha.org> We have a square board with a vice screwed to it. Heavy, but it does the job. We can stand up in the sink and decontaminate. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, April 11, 2007 12:33 PM To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bone saw "(Meanwhile, down the hall, the radiology department just bought a 64-slice CT scanner, their toy of the year. The red-haired stepchild....)" It's one of the strangest facts of life that radiologists can get what they want, just to see shadows. Millions - no problem, but a few measly thousand spent in pathology, where the real diagnoses are made - sharp intake of breath with a "not so sure about that". Terry PS The recurring problem of sawing bones drives me nuts. The sawing bit is easy, you just need a saw, it's the holding of the bone that's the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: 11 April 2007 17:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Bone saw Most surgical pathologists cut bone with an electric Stryker oscillating saw, since their autopsy service usually has one. These really aren't suitable for surgical pathology, since it's difficult to hold the specimen down, particularly if you don't have a bench vise. I've always relied on hand-held saws. The old Satterlee amputation saw, of Civil War (1861-65) vintage, is the usual saw available. (I've twice seen these in Civil War re-enactor hospitals, complete with chrome plating, right like they come from whatever Lipshaw is called this week.) Many small surgical pathology services have no saw of any kind. When I work one of these services, I go to a hardware store and spend five dollars on a hacksaw, and leave it behind when I go. An elegant variation on the hacksaw is the "Sawbones", which combines a sturdy clamp with two parallel long hacksaw blades that cut a slab of a femoral head a few mm thick. It's rather overpriced at about $500, prohibitively expensive for something the pathologist is actually going to have his hands on. The bone pathologist at Johns Hopkins recommends a tabletop scroll saw. These cost about $200. They're supposed to be quite safe, but they have too large a footprint for the usual cramped surgical pathology lab. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From japoteete <@t> saintfrancis.com Wed Apr 11 11:40:35 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Wed Apr 11 11:40:47 2007 Subject: [Histonet] Re: Bone saw In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2BC@TRFT-EX01.xRothGen.nhs.uk> Message-ID: I know what you mean! Here we can't get a tissue processor because the Breast Radiology folks "forgot" to order all of the required components for their digital scanner. Again, millions versus thousands. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, April 11, 2007 11:33 AM To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bone saw "(Meanwhile, down the hall, the radiology department just bought a 64-slice CT scanner, their toy of the year. The red-haired stepchild....)" It's one of the strangest facts of life that radiologists can get what they want, just to see shadows. Millions - no problem, but a few measly thousand spent in pathology, where the real diagnoses are made - sharp intake of breath with a "not so sure about that". Terry PS The recurring problem of sawing bones drives me nuts. The sawing bit is easy, you just need a saw, it's the holding of the bone that's the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: 11 April 2007 17:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Bone saw Most surgical pathologists cut bone with an electric Stryker oscillating saw, since their autopsy service usually has one. These really aren't suitable for surgical pathology, since it's difficult to hold the specimen down, particularly if you don't have a bench vise. I've always relied on hand-held saws. The old Satterlee amputation saw, of Civil War (1861-65) vintage, is the usual saw available. (I've twice seen these in Civil War re-enactor hospitals, complete with chrome plating, right like they come from whatever Lipshaw is called this week.) Many small surgical pathology services have no saw of any kind. When I work one of these services, I go to a hardware store and spend five dollars on a hacksaw, and leave it behind when I go. An elegant variation on the hacksaw is the "Sawbones", which combines a sturdy clamp with two parallel long hacksaw blades that cut a slab of a femoral head a few mm thick. It's rather overpriced at about $500, prohibitively expensive for something the pathologist is actually going to have his hands on. The bone pathologist at Johns Hopkins recommends a tabletop scroll saw. These cost about $200. They're supposed to be quite safe, but they have too large a footprint for the usual cramped surgical pathology lab. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson_jm <@t> cimar.org Wed Apr 11 11:56:40 2007 From: wilson_jm <@t> cimar.org (Jonathan Wilson) Date: Wed Apr 11 11:57:06 2007 Subject: [Histonet] quantification of apical labeling in intestinal brushboarder References: Message-ID: <00ad01c77c5a$62753da0$4352d258@COBITIS> Hello Histonetters, I am looking for advice on quantify apical (brush boarder) immunolableing of intestine. The apical labeling is not uniform and I would therefore like a means of randomly sampling. I was wondering if it was acceptable to use an overlay grid and measure fluorescence intensity at the points where the grid lines intersected on randomly orientated micrographs. I know that this is used for stereology for morphometrics but haven't come across its use for this type of quantification (in my limited reading). My second problem that arises from this is what type of grid would be most appropriate and where I could get a copy or better yet how to produce one. Unfortunately, I do not have access to a good library (and therefore stereology books) and access to internet journals is limited. Any help would be greatly appreciated. Sincerely, Jon Jonathan Wilson (PhD) Ecofisiologia CIMAR Rua dos Bragas 289 4050-123 Porto Portugal office 351 22 340 1809 lab 351 22 340 1834 fax 351 22 339 0608 From jqb7 <@t> cdc.gov Wed Apr 11 12:23:30 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 11 12:23:55 2007 Subject: [Histonet] FW: HTL question Message-ID: All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? From bruce.webber <@t> cefe.cnrs.fr Wed Apr 11 12:25:50 2007 From: bruce.webber <@t> cefe.cnrs.fr (Bruce Webber) Date: Wed Apr 11 12:26:05 2007 Subject: [Histonet] In situ hybridization on insects: fixing, processing & cuticle softening advice In-Reply-To: <59688E3227A2204CBC05459074547F951C8753@LMMAIL.lmhosp.local> References: <46151805.8020602@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8750@LMMAIL.lmhosp.local> <461BBB0A.8040609@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8753@LMMAIL.lmhosp.local> Message-ID: <461D1A1E.7020709@cefe.cnrs.fr> Perhaps I shouldn't have sent my request for information so close to the Easter holidays.... I'm yet to receive any feedback on my query email of April 5th, so if anyone has some input on any of the issues I would greatly appreciate it! With thanks in advance, Bruce From TownsendD <@t> childrensdayton.org Wed Apr 11 12:35:10 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Apr 11 12:35:55 2007 Subject: [Histonet] FW: HTL question Message-ID: As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores From JCollins <@t> palmbeachpath.com Wed Apr 11 12:36:07 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Wed Apr 11 12:36:16 2007 Subject: [Histonet] HTL Question Message-ID: Although my first thought was that "C" was correct, on second thought, I think it probably is "D" as they stain the same color as the background (counterstain) and therefore tend to blend in. Judy From jlinda <@t> ces.clemson.edu Wed Apr 11 12:40:17 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Wed Apr 11 12:39:50 2007 Subject: [Histonet] Simple device for holding bones Message-ID: <5.2.1.1.2.20070411132713.01f43c98@mailhost.ces.clemson.edu> Terry, You stated: "PS The recurring problem of sawing bones drives me nuts. The sawing bit is easy, you just need a saw, it's the holding of the bone that's the problem." Next time you are out shopping, pick up a melon ball device in assorted sizes. They are perfect for holding a femoral head to guide into the table top band saw or while using the Stryker Saw. They are also great for holding irregular shaped bones such as clavicles, etc. From the kitchen to the lab... :-) Linda PS: Now, if we could just figure out how to put those nifty stir bars into our home cook pots so we would not have to stand and stir...hmmm! Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From bruce.webber <@t> cefe.cnrs.fr Wed Apr 11 12:40:06 2007 From: bruce.webber <@t> cefe.cnrs.fr (Bruce Webber) Date: Wed Apr 11 12:40:21 2007 Subject: [Histonet] In situ hybridization on insects: fixing, processing & cuticle softening advice In-Reply-To: <461D1A1E.7020709@cefe.cnrs.fr> References: <46151805.8020602@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8750@LMMAIL.lmhosp.local> <461BBB0A.8040609@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8753@LMMAIL.lmhosp.local> <461D1A1E.7020709@cefe.cnrs.fr> Message-ID: <461D1D76.8050409@cefe.cnrs.fr> ... and here is the original email, given that the previous email does not seem to link up to the original message thread. On 05/04/2007 16:38, Bruce Webber wrote: > Hi Histonetters, > > I am looking for advice on preparing and processing small (c. 2-3mm > long) adult ants (i.e. with a well-formed chitinous exoskeleton) for > in situ hybridization (ISH) detection of bacteria (using DNA probes to > target RNA). The ants are transverse sectioned into 3 parts before > fixing to allow greater penetration into each of the 3 main body cavities. > > I've trawled the archives for various options and have consulted my > histological mentor, Bruce Abaloz, but after not finding what I was > looking for, I would greatly appreciate advice, opinions and > recommendations from others. I do promise to post a summary of any > responses together with any methodological tips I found along the way! > > The main problem I see is dealing with the cuticle to allow for good > sectioning (5-8um), but using chemicals & methods that don't interfere > with ISH, damage the target RNA, or interfere with the efficiency of > the ISH probes. My understanding is that the following factors need > to be addressed: > > 1) Fixing the tissue: > The only available material available at this stage was fixed in 2% > gluteraldehyde (12h at 4?C) and then stored (> 3 weeks) in 80% EtOH > (meaning that gut excision is not an option due to brittle material). > In the future, 4% paraformaldehyde, 10% neutral buffered formalin or > just straight into 70% EtOH (with slightly shorter storage times) > could also be considered as options if people feel that they may be > better (the latter option would certainly increase the availability of > material). > > 2) Softening the cuticle: > IMHO this is the biggest potential area for chemical > damage/interference with ISH. Proposals I am aware of that would not > be compatible with ISH due to acidic damage of the RNA are [1] > Perenyi's fluid (4:3:3, 10% nitric acid:100% EtOH:0.05% chromic > acid); [2] Diaphanol (50% glacial acetic acid saturated with ClO2); > and [3] Bouin's fixative (3:1:2, saturated picric > acid:formaldehyde:glacial acetic acid). > > However, other suggestions from previous posts which I know very > little about include [4] chloral hydrate processing (after tissue > dehydration, melt equal parts of chloral hydrate & phenol and immerse > tissue for up to 7 days, followed by chloroform as an intermediary > agent); [5] Mollifex Gurr (glycerol, phenol, acetone and EtOH > solution, applied to the cutting surface of the paraffin block); [6] > Nair (as in the hair removal cream - thioglycolate salts & calcium > hydroxide, between fixing and processing, soak the tissue for c. > 24hrs); and [7] Phenol (after fixation, soak fixed specimens in 4% > phenol (in 80% EtOH) for 24 hours). Lastly, there is 1% DMSO (added > to the fixative), but given that the ant is chopped into 3 sections, > this may not be necessary to ensure adequate penetration (but does > DMSO also improve sectioning?). > > 3) Processing the tissue: > The ongoing debate of xylene v histoclear (or histosolve)..... > Histoclear is probably safer, but does xylene produce better results? > Has anyone had experience with either on similar invertebrate > material? Others have suggested using isopropyl alcohol (instead of > EtOH) for dehydration as is tends to have a less hardening effect on > the tissue. > > 4) Material for embedding: > I've seen it argued that tougher material such as that containing > chitinous exoskeletons benefits from using a harder embedding medium > (e.g. TissuePrep 2 from Fisher) or one with better infiltration (e.g. > Paraplast X-tra) over standard paraffin. I've also read that > Paraplast Plus (containing DMSO) makes sectioning of cuticles easier. > Any opinions/recommendations/experience with similar material and any > of these embedding mediums would be appreciated! > > Thank you all in advance for your help with this matter (I apologise > for the length of this post!). > > Bruce Webber > Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, France > > (Key words: cuticle, exoskeleton, chitin, insect, invertebrate, ant, > in situ hybridization, ISH, softening.) From liz <@t> premierlab.com Wed Apr 11 13:06:48 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Apr 11 12:53:45 2007 Subject: [Histonet] FW: HTL question In-Reply-To: Message-ID: <000201c77c64$2c5d8a10$0d00a8c0@domain.Premier> ASCP study guide Some of the questions that are in the HT/HTL study from ASCP are questions that used to be on the exam but were removed because they did not perform well for a variety of reasons. They did not differentiate between knowledgeable candidates and others that did not know as much, or perhaps there was more than one correct answer, but they still give the candidate an idea of how the questions are written, etc. This question was probably removed from the exam and is in the booklet for the same reason it is under discussion now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 11:35 AM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jqb7 <@t> cdc.gov Wed Apr 11 12:55:20 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 11 12:55:36 2007 Subject: [Histonet] FW: HTL question References: <000201c77c64$2c5d8a10$0d00a8c0@domain.Premier> Message-ID: Good point. It definitely seems like a poorly designed question. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, April 11, 2007 2:07 PM To: 'Dolores Townsend'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: HTL question ASCP study guide Some of the questions that are in the HT/HTL study from ASCP are questions that used to be on the exam but were removed because they did not perform well for a variety of reasons. They did not differentiate between knowledgeable candidates and others that did not know as much, or perhaps there was more than one correct answer, but they still give the candidate an idea of how the questions are written, etc. This question was probably removed from the exam and is in the booklet for the same reason it is under discussion now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 11:35 AM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Apr 11 13:21:43 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Apr 11 13:21:53 2007 Subject: [Histonet] FW: HTL question In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C66@LSRIEXCH1.lsmaster.lifespan.org> It's a poor question because the last choice is ambiguous. When you read "are never demonstrated" you naturally take that to mean "cannot be seen". However, we know that fungi can in fact be seen in an H&E section. Therefore they are "demonstrated" to some degree. But they are not specifically demonstrated in a way that makes them obvious against the background. From CIngles <@t> uwhealth.org Wed Apr 11 13:29:11 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Apr 11 13:34:00 2007 Subject: [Histonet] Re: Bone saw References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120021@uwhis-xchng3.uwhis.hosp.wisc.edu> I know what you mean! Here we can't get a tissue processor because the Breast Radiology folks "forgot" to order all of the required components for their digital scanner. Again, millions versus thousands. Maybe we all need to hire publicity consultants or agents to get the word out... :) Claire Ingles UW Hospital Madison WI From oshel1pe <@t> cmich.edu Wed Apr 11 13:48:09 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Apr 11 13:48:35 2007 Subject: [Histonet] Re: Bone saw Message-ID: Rubber bracelets, H&E colored. Enjoy the snow today! 2 years ago, I would've been on the 10th floor of Animal Sciences, and I suspect unable to see the UW Hospital for the flakes. Phll > > > > >I know what you mean! Here we can't get a tissue processor because the >Breast Radiology folks "forgot" to order all of the required components >for their digital scanner. Again, millions versus thousands. > > > Maybe we all need to hire publicity consultants or agents to get >the word out... :) > > > >Claire Ingles > >UW Hospital > >Madison WI -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From akemiat3377 <@t> yahoo.com Wed Apr 11 14:00:20 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Apr 11 14:00:28 2007 Subject: [Histonet] Re: Bone saw In-Reply-To: Message-ID: <912935.16875.qm@web31302.mail.mud.yahoo.com> Yeah, Snow Brizzard in the middle of April...After coming back from 75 degree weather. I feel like like Dorothy clicking her heels.... THERE'S NO PLACE LIKE HOME!! THERE'S NO PLACE LIKE HOME!! Won't be long now. Akemi --- Philip Oshel wrote: > Rubber bracelets, H&E colored. > Enjoy the snow today! 2 years ago, I would've been > on the 10th floor > of Animal Sciences, and I suspect unable to see the > UW Hospital for > the flakes. > > Phll > > > > > > > > > > >I know what you mean! Here we can't get a tissue > processor because the > >Breast Radiology folks "forgot" to order all of the > required components > >for their digital scanner. Again, millions versus > thousands. > > > > > > Maybe we all need to hire publicity consultants > or agents to get > >the word out... :) > > > > > > > >Claire Ingles > > > >UW Hospital > > > >Madison WI > -- > Philip Oshel > Microscopy Facility Supervisor > Biology Department > 024C Brooks Hall > Central Michigan University > Mt. Pleasant, MI 48859 > voice: (989) 774-3576 > dept. fax: (989) 774-3462 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Wed Apr 11 14:22:29 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 11 14:22:45 2007 Subject: [Histonet] FW: HTL question In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C5@EMAIL.archildrens.org> I disagree. An experienced pathologist will see and know fungus on an H&E. He/She does not need another stain, billed to the patient, for confirmation. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 12:35 PM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From oshel1pe <@t> cmich.edu Wed Apr 11 14:34:13 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Apr 11 14:34:23 2007 Subject: [Histonet] Re: Bone saw In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FAEAB7@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FAEAB7@sjhaexc02.sjha.org> Message-ID: Mid-mitten Michigan. The way this weather system is moving, you may yet see snow. (I like Canada, but I could do without the exports from Nunavut and the Northwest Territories.) And I only see the snow if I crawl up out of the dungeon where us EM trolls are generally kept. Phil >Hey Phil, where are you? I would love to see some snow.. rain here in >Hotlanta. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip >Oshel >Sent: Wednesday, April 11, 2007 1:48 PM >To: Histonet@Pathology.swmed.edu >Subject: RE: [Histonet] Re: Bone saw > >Rubber bracelets, H&E colored. >Enjoy the snow today! 2 years ago, I would've been on the 10th floor >of Animal Sciences, and I suspect unable to see the UW Hospital for >the flakes. > >Phll > >> >> >> >> >>I know what you mean! Here we can't get a tissue processor because the >>Breast Radiology folks "forgot" to order all of the required components >>for their digital scanner. Again, millions versus thousands. >> >> >> Maybe we all need to hire publicity consultants or agents to get >>the word out... :) >> >> >> >>Claire Ingles >> >>UW Hospital >> >>Madison WI >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >024C Brooks Hall >Central Michigan University >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message >may be privileged and is confidential information intended for the >use of the addressee listed above. If you are neither the intended >recipient nor the employee or agent responsible for delivering this >message to the intended recipient, you are hereby notified that any >disclosure, copying, distribution or the taking of any action in >reliance on the contents of this information is strictly prohibited. >If you have received this communication in error, please notify us >immediately by replying to the message and deleting it from your >computer. Thank you. Saint Joseph's Health System, Inc. From akemiat3377 <@t> yahoo.com Wed Apr 11 14:48:57 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Apr 11 14:49:03 2007 Subject: [Histonet] FW: HTL question Can diagnose fungus from H&E In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C5@EMAIL.archildrens.org> Message-ID: <862289.72421.qm@web31315.mail.mud.yahoo.com> You have opened Pandora's Box!! One thing I have learned, once it's in writing, no turning back! --- "Horn, Hazel V" wrote: > I disagree. An experienced pathologist will see and > know fungus on an > H&E. He/She does not need another stain, billed to > the patient, for > confirmation. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dolores > Townsend > Sent: Wednesday, April 11, 2007 12:35 PM > To: Jeanine (CDC/CCID/NCZVED) Bartlett; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] FW: HTL question > > As for a lot of tests, the answer is a bit tricky > and confusing, but D > is right: although the pathologist can see something > that MIGHT be > fungus on the routine stain, (s)he will ask for a > special stain (GMS or > other) to confirm the presence of fungus. Hence, D. > Fungi are never > demonstrated with routine procedure. You will need > the special stain to > be sure. This is true for most special stains: the > pathologist sees > something on the routine stain, but it is not > conclusive, so special > stains are needed to confirm the diagnostic. > Dolores > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------------ > The information contained in this message may be > privileged and confidential > and protected from disclosure. If the reader of this > message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are hereby > notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this communication > in error, please notify > us immediately by replying to the message and > deleting it from your computer. > Thank you. > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Wed Apr 11 15:02:23 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 11 15:02:41 2007 Subject: [Histonet] FW: HTL question Can diagnose fungus from H&E In-Reply-To: <862289.72421.qm@web31315.mail.mud.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C8@EMAIL.archildrens.org> Oops.... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Wednesday, April 11, 2007 2:49 PM To: Horn, Hazel V; Dolores Townsend; Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E You have opened Pandora's Box!! One thing I have learned, once it's in writing, no turning back! --- "Horn, Hazel V" wrote: > I disagree. An experienced pathologist will see and > know fungus on an > H&E. He/She does not need another stain, billed to > the patient, for > confirmation. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dolores > Townsend > Sent: Wednesday, April 11, 2007 12:35 PM > To: Jeanine (CDC/CCID/NCZVED) Bartlett; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] FW: HTL question > > As for a lot of tests, the answer is a bit tricky > and confusing, but D > is right: although the pathologist can see something > that MIGHT be > fungus on the routine stain, (s)he will ask for a > special stain (GMS or > other) to confirm the presence of fungus. Hence, D. > Fungi are never > demonstrated with routine procedure. You will need > the special stain to > be sure. This is true for most special stains: the > pathologist sees > something on the routine stain, but it is not > conclusive, so special > stains are needed to confirm the diagnostic. > Dolores > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------ ------ > The information contained in this message may be > privileged and confidential > and protected from disclosure. If the reader of this > message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are hereby > notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this communication > in error, please notify > us immediately by replying to the message and > deleting it from your computer. > Thank you. > ======================================================================== ====== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From gcallis <@t> montana.edu Wed Apr 11 15:04:49 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Apr 11 15:04:48 2007 Subject: [Histonet] Seasonal snap freezing noses and ears in Montana In-Reply-To: References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FAEAB7@sjhaexc02.sjha.org> Message-ID: <6.0.0.22.1.20070411134619.01b0bf58@gemini.msu.montana.edu> Ah, you guys have it WARM, so don't be wussies! It has been snowing here all week. Come visit Montana where our annual Easter Bunny snowstorm only dropped 8 inches heavy wet white UGH! this year (normal is ~ 2 ft!) Temperatures plunged to 20F - 25F with winds making the annual Easter egg hunt a ear and nose snap freezing experience. The front yard mini glacier should be taken out with Global Warming by end of week though. Springtime in the Rockies is sooo much fun at times. Gayle Callis From Frozen Reaches of Montana! At 01:34 PM 4/11/2007, you wrote: >Mid-mitten Michigan. The way this weather system is moving, you may yet >see snow. (I like Canada, but I could do without the exports from Nunavut >and the Northwest Territories.) > >And I only see the snow if I crawl up out of the dungeon where us EM >trolls are generally kept. >Phil > >>Hey Phil, where are you? I would love to see some snow.. rain here in >>Hotlanta. >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip >>Oshel >>Sent: Wednesday, April 11, 2007 1:48 PM >>To: Histonet@Pathology.swmed.edu >>Subject: RE: [Histonet] Re: Bone saw >> >>Rubber bracelets, H&E colored. >>Enjoy the snow today! 2 years ago, I would've been on the 10th floor >>of Animal Sciences, and I suspect unable to see the UW Hospital for >>the flakes. >> >>Phll From oshel1pe <@t> cmich.edu Wed Apr 11 15:08:45 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Apr 11 15:08:59 2007 Subject: [Histonet] web resource for histology Message-ID: Listers, I just came upon this site and thought I'd pass it on. Hope it's not repeating a well-known resource. http://courseweb.edteched.uottawa.ca/Medicine-histology/Default_En.htm Also available in French. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From Barry.R.Rittman <@t> uth.tmc.edu Wed Apr 11 15:12:54 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Apr 11 15:13:03 2007 Subject: [Histonet] Re: Bone saw In-Reply-To: <912935.16875.qm@web31302.mail.mud.yahoo.com> Message-ID: While I don't wish to seem an old petarder, most of the several emails we have seen re bone saws have nothing to do with bone saws. How about decreasing the amount of interpersonal chatter that is being distributed to all on the Histonet so that we don't have to waste our time with opening and deleting all these? Thanks for your consideration Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, April 11, 2007 2:00 PM To: Philip Oshel; Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Re: Bone saw Yeah, Snow Brizzard in the middle of April...After coming back from 75 degree weather. I feel like like Dorothy clicking her heels.... THERE'S NO PLACE LIKE HOME!! THERE'S NO PLACE LIKE HOME!! Won't be long now. Akemi --- Philip Oshel wrote: > Rubber bracelets, H&E colored. > Enjoy the snow today! 2 years ago, I would've been > on the 10th floor > of Animal Sciences, and I suspect unable to see the > UW Hospital for > the flakes. > > Phll > > > > > > > > > > >I know what you mean! Here we can't get a tissue > processor because the > >Breast Radiology folks "forgot" to order all of the > required components > >for their digital scanner. Again, millions versus > thousands. > > > > > > Maybe we all need to hire publicity consultants > or agents to get > >the word out... :) > > > > > > > >Claire Ingles > > > >UW Hospital > > > >Madison WI > -- > Philip Oshel > Microscopy Facility Supervisor > Biology Department > 024C Brooks Hall > Central Michigan University > Mt. Pleasant, MI 48859 > voice: (989) 774-3576 > dept. fax: (989) 774-3462 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Apr 11 15:19:23 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 11 15:19:49 2007 Subject: [Histonet] web resource for histology In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C9@EMAIL.archildrens.org> I browsed this site a bit and it is good. I'll spend more time there when I can. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Wednesday, April 11, 2007 3:09 PM To: Histonet@Pathology.swmed.edu Subject: [Histonet] web resource for histology Listers, I just came upon this site and thought I'd pass it on. Hope it's not repeating a well-known resource. http://courseweb.edteched.uottawa.ca/Medicine-histology/Default_En.htm Also available in French. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Rcartun <@t> harthosp.org Wed Apr 11 17:11:53 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Apr 11 17:12:06 2007 Subject: [Histonet] When staining IHC's, are labs putting control tissue on every slide? In-Reply-To: References: <7F2A2AE306CE254DB7279E86A51A7406125E33@CMROCEX01.cellmarque.local> Message-ID: <461D24E902000077000053E6@gwmail.harthosp.org> No, our positive control tissues are placed on separate slides. We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Happel, James F." 04/10/07 4:29 PM >>> Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From jqb7 <@t> cdc.gov Wed Apr 11 17:20:25 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 11 17:20:29 2007 Subject: [Histonet] FW: HTL question References: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C5@EMAIL.archildrens.org> Message-ID: Absolutely! Sometimes you need that special for confirmation but definteley not always. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wed 4/11/2007 3:22 PM To: Dolores Townsend; Bartlett, Jeanine (CDC/CCID/NCZVED); histonet@lists.utsouthwestern.edu Cc: Subject: RE: [Histonet] FW: HTL question I disagree. An experienced pathologist will see and know fungus on an H&E. He/She does not need another stain, billed to the patient, for confirmation. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 12:35 PM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Rcartun <@t> harthosp.org Wed Apr 11 17:21:59 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Apr 11 17:22:17 2007 Subject: [Histonet] FW: HTL question In-Reply-To: References: Message-ID: <461D274702000077000053EF@gwmail.harthosp.org> I would have picked "C" as well. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 04/11/07 1:23 PM >>> All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From shirley.jen <@t> roche.com Wed Apr 11 18:24:22 2007 From: shirley.jen <@t> roche.com (Jen, Shirley) Date: Wed Apr 11 18:24:39 2007 Subject: [Histonet] Job Opening In-Reply-To: <200704112227.FBE98232@mailgate4.roche.com> References: <200704112227.FBE98232@mailgate4.roche.com> Message-ID: <6A39BF27EAB27743887E0425BD51196B0499AF71@rplmsem1.nala.roche.com> Research Associate position available at Roche Palo Alto, California. This position will function as a histotechnologist in the Non-Clincial Drug Safety group, performing histology, from wet tissue trimming to staining, immunohistochemistry, and imaging tasks to support research and investigative toxicology studies. Please send resume to shirley.jen@roche.com. Shirley Jen Research Supervisor, Histology Pathology Department Roche Palo Alto LLC 3431 Hillview Avenue Palo Alto, CA ?94304 Phone:? 650-852-1810 Fax:? 650-852-1098 e-mail:? shirley.jen@roche.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, April 11, 2007 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 41, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: HTL question (Bartlett, Jeanine (CDC/CCID/NCZVED)) 2. Re: In situ hybridization on insects: fixing, processing & cuticle softening advice (Bruce Webber) 3. Re: FW: HTL question (Dolores Townsend) 4. HTL Question (Judy Collins) 5. Simple device for holding bones (Linda Jenkins) 6. Re: In situ hybridization on insects: fixing, processing & cuticle softening advice (Bruce Webber) 7. RE: FW: HTL question (Liz Chlipala) 8. RE: FW: HTL question (Bartlett, Jeanine (CDC/CCID/NCZVED)) 9. RE: FW: HTL question (Monfils, Paul) 10. RE: Re: Bone saw (Ingles Claire) 11. RE: Re: Bone saw (Philip Oshel) 12. RE: Re: Bone saw (Akemi Allison-Tacha) 13. RE: FW: HTL question (Horn, Hazel V) 14. RE: Re: Bone saw (Philip Oshel) 15. RE: FW: HTL question Can diagnose fungus from H&E (Akemi Allison-Tacha) 16. RE: FW: HTL question Can diagnose fungus from H&E (Horn, Hazel V) 17. Seasonal snap freezing noses and ears in Montana (Gayle Callis) 18. web resource for histology (Philip Oshel) 19. RE: Re: Bone saw (Rittman, Barry R) 20. RE: web resource for histology (Horn, Hazel V) 21. Re: When staining IHC's, are labs putting control tissue on every slide? (Richard Cartun) 22. RE: FW: HTL question (Bartlett, Jeanine (CDC/CCID/NCZVED)) 23. Re: FW: HTL question (Richard Cartun) ---------------------------------------------------------------------- Message: 1 Date: Wed, 11 Apr 2007 13:23:30 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" Subject: [Histonet] FW: HTL question To: Message-ID: Content-Type: text/plain; charset="us-ascii" All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? ------------------------------ Message: 2 Date: Wed, 11 Apr 2007 18:25:50 +0100 From: Bruce Webber Subject: Re: [Histonet] In situ hybridization on insects: fixing, processing & cuticle softening advice To: Histonet Message-ID: <461D1A1E.7020709@cefe.cnrs.fr> Content-Type: text/plain; charset=ISO-8859-1 Perhaps I shouldn't have sent my request for information so close to the Easter holidays.... I'm yet to receive any feedback on my query email of April 5th, so if anyone has some input on any of the issues I would greatly appreciate it! With thanks in advance, Bruce ------------------------------ Message: 3 Date: Wed, 11 Apr 2007 13:35:10 -0400 From: "Dolores Townsend" Subject: Re: [Histonet] FW: HTL question To: "Jeanine (CDC/CCID/NCZVED) Bartlett" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores ------------------------------ Message: 4 Date: Wed, 11 Apr 2007 13:36:07 -0400 From: "Judy Collins" Subject: [Histonet] HTL Question To: Message-ID: Content-Type: text/plain; charset="us-ascii" Although my first thought was that "C" was correct, on second thought, I think it probably is "D" as they stain the same color as the background (counterstain) and therefore tend to blend in. Judy ------------------------------ Message: 5 Date: Wed, 11 Apr 2007 13:40:17 -0400 From: Linda Jenkins Subject: [Histonet] Simple device for holding bones To: histonet@lists.utsouthwestern.edu Message-ID: <5.2.1.1.2.20070411132713.01f43c98@mailhost.ces.clemson.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Terry, You stated: "PS The recurring problem of sawing bones drives me nuts. The sawing bit is easy, you just need a saw, it's the holding of the bone that's the problem." Next time you are out shopping, pick up a melon ball device in assorted sizes. They are perfect for holding a femoral head to guide into the table top band saw or while using the Stryker Saw. They are also great for holding irregular shaped bones such as clavicles, etc. From the kitchen to the lab... :-) Linda PS: Now, if we could just figure out how to put those nifty stir bars into our home cook pots so we would not have to stand and stir...hmmm! Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm ------------------------------ Message: 6 Date: Wed, 11 Apr 2007 18:40:06 +0100 From: Bruce Webber Subject: Re: [Histonet] In situ hybridization on insects: fixing, processing & cuticle softening advice To: Histonet Message-ID: <461D1D76.8050409@cefe.cnrs.fr> Content-Type: text/plain; charset=ISO-8859-1 ... and here is the original email, given that the previous email does not seem to link up to the original message thread. On 05/04/2007 16:38, Bruce Webber wrote: > Hi Histonetters, > > I am looking for advice on preparing and processing small (c. 2-3mm > long) adult ants (i.e. with a well-formed chitinous exoskeleton) for > in situ hybridization (ISH) detection of bacteria (using DNA probes to > target RNA). The ants are transverse sectioned into 3 parts before > fixing to allow greater penetration into each of the 3 main body cavities. > > I've trawled the archives for various options and have consulted my > histological mentor, Bruce Abaloz, but after not finding what I was > looking for, I would greatly appreciate advice, opinions and > recommendations from others. I do promise to post a summary of any > responses together with any methodological tips I found along the way! > > The main problem I see is dealing with the cuticle to allow for good > sectioning (5-8um), but using chemicals & methods that don't interfere > with ISH, damage the target RNA, or interfere with the efficiency of > the ISH probes. My understanding is that the following factors need > to be addressed: > > 1) Fixing the tissue: > The only available material available at this stage was fixed in 2% > gluteraldehyde (12h at 4?C) and then stored (> 3 weeks) in 80% EtOH > (meaning that gut excision is not an option due to brittle material). > In the future, 4% paraformaldehyde, 10% neutral buffered formalin or > just straight into 70% EtOH (with slightly shorter storage times) > could also be considered as options if people feel that they may be > better (the latter option would certainly increase the availability of > material). > > 2) Softening the cuticle: > IMHO this is the biggest potential area for chemical > damage/interference with ISH. Proposals I am aware of that would not > be compatible with ISH due to acidic damage of the RNA are [1] > Perenyi's fluid (4:3:3, 10% nitric acid:100% EtOH:0.05% chromic > acid); [2] Diaphanol (50% glacial acetic acid saturated with ClO2); > and [3] Bouin's fixative (3:1:2, saturated picric > acid:formaldehyde:glacial acetic acid). > > However, other suggestions from previous posts which I know very > little about include [4] chloral hydrate processing (after tissue > dehydration, melt equal parts of chloral hydrate & phenol and immerse > tissue for up to 7 days, followed by chloroform as an intermediary > agent); [5] Mollifex Gurr (glycerol, phenol, acetone and EtOH > solution, applied to the cutting surface of the paraffin block); [6] > Nair (as in the hair removal cream - thioglycolate salts & calcium > hydroxide, between fixing and processing, soak the tissue for c. > 24hrs); and [7] Phenol (after fixation, soak fixed specimens in 4% > phenol (in 80% EtOH) for 24 hours). Lastly, there is 1% DMSO (added > to the fixative), but given that the ant is chopped into 3 sections, > this may not be necessary to ensure adequate penetration (but does > DMSO also improve sectioning?). > > 3) Processing the tissue: > The ongoing debate of xylene v histoclear (or histosolve)..... > Histoclear is probably safer, but does xylene produce better results? > Has anyone had experience with either on similar invertebrate > material? Others have suggested using isopropyl alcohol (instead of > EtOH) for dehydration as is tends to have a less hardening effect on > the tissue. > > 4) Material for embedding: > I've seen it argued that tougher material such as that containing > chitinous exoskeletons benefits from using a harder embedding medium > (e.g. TissuePrep 2 from Fisher) or one with better infiltration (e.g. > Paraplast X-tra) over standard paraffin. I've also read that > Paraplast Plus (containing DMSO) makes sectioning of cuticles easier. > Any opinions/recommendations/experience with similar material and any > of these embedding mediums would be appreciated! > > Thank you all in advance for your help with this matter (I apologise > for the length of this post!). > > Bruce Webber > Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, France > > (Key words: cuticle, exoskeleton, chitin, insect, invertebrate, ant, > in situ hybridization, ISH, softening.) ------------------------------ Message: 7 Date: Wed, 11 Apr 2007 12:06:48 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] FW: HTL question To: "'Dolores Townsend'" , Message-ID: <000201c77c64$2c5d8a10$0d00a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" ASCP study guide Some of the questions that are in the HT/HTL study from ASCP are questions that used to be on the exam but were removed because they did not perform well for a variety of reasons. They did not differentiate between knowledgeable candidates and others that did not know as much, or perhaps there was more than one correct answer, but they still give the candidate an idea of how the questions are written, etc. This question was probably removed from the exam and is in the booklet for the same reason it is under discussion now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 11:35 AM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com ------------------------------ Message: 8 Date: Wed, 11 Apr 2007 13:55:20 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" Subject: RE: [Histonet] FW: HTL question To: "Liz Chlipala" , "Dolores Townsend" , Message-ID: Content-Type: text/plain; charset="us-ascii" Good point. It definitely seems like a poorly designed question. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, April 11, 2007 2:07 PM To: 'Dolores Townsend'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: HTL question ASCP study guide Some of the questions that are in the HT/HTL study from ASCP are questions that used to be on the exam but were removed because they did not perform well for a variety of reasons. They did not differentiate between knowledgeable candidates and others that did not know as much, or perhaps there was more than one correct answer, but they still give the candidate an idea of how the questions are written, etc. This question was probably removed from the exam and is in the booklet for the same reason it is under discussion now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 11:35 AM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 11 Apr 2007 14:21:43 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] FW: HTL question To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C66@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" It's a poor question because the last choice is ambiguous. When you read "are never demonstrated" you naturally take that to mean "cannot be seen". However, we know that fungi can in fact be seen in an H&E section. Therefore they are "demonstrated" to some degree. But they are not specifically demonstrated in a way that makes them obvious against the background. ------------------------------ Message: 10 Date: Wed, 11 Apr 2007 13:29:11 -0500 From: "Ingles Claire" Subject: RE: [Histonet] Re: Bone saw To: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120021@uwhis-xchng3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" I know what you mean! Here we can't get a tissue processor because the Breast Radiology folks "forgot" to order all of the required components for their digital scanner. Again, millions versus thousands. Maybe we all need to hire publicity consultants or agents to get the word out... :) Claire Ingles UW Hospital Madison WI ------------------------------ Message: 11 Date: Wed, 11 Apr 2007 14:48:09 -0400 From: Philip Oshel Subject: RE: [Histonet] Re: Bone saw To: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Rubber bracelets, H&E colored. Enjoy the snow today! 2 years ago, I would've been on the 10th floor of Animal Sciences, and I suspect unable to see the UW Hospital for the flakes. Phll > > > > >I know what you mean! Here we can't get a tissue processor because the >Breast Radiology folks "forgot" to order all of the required components >for their digital scanner. Again, millions versus thousands. > > > Maybe we all need to hire publicity consultants or agents to get >the word out... :) > > > >Claire Ingles > >UW Hospital > >Madison WI -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 ------------------------------ Message: 12 Date: Wed, 11 Apr 2007 12:00:20 -0700 (PDT) From: Akemi Allison-Tacha Subject: RE: [Histonet] Re: Bone saw To: Philip Oshel , Histonet@Pathology.swmed.edu Message-ID: <912935.16875.qm@web31302.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yeah, Snow Brizzard in the middle of April...After coming back from 75 degree weather. I feel like like Dorothy clicking her heels.... THERE'S NO PLACE LIKE HOME!! THERE'S NO PLACE LIKE HOME!! Won't be long now. Akemi --- Philip Oshel wrote: > Rubber bracelets, H&E colored. > Enjoy the snow today! 2 years ago, I would've been > on the 10th floor > of Animal Sciences, and I suspect unable to see the > UW Hospital for > the flakes. > > Phll > > > > > > > > > > >I know what you mean! Here we can't get a tissue > processor because the > >Breast Radiology folks "forgot" to order all of the > required components > >for their digital scanner. Again, millions versus > thousands. > > > > > > Maybe we all need to hire publicity consultants > or agents to get > >the word out... :) > > > > > > > >Claire Ingles > > > >UW Hospital > > > >Madison WI > -- > Philip Oshel > Microscopy Facility Supervisor > Biology Department > 024C Brooks Hall > Central Michigan University > Mt. Pleasant, MI 48859 > voice: (989) 774-3576 > dept. fax: (989) 774-3462 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Wed, 11 Apr 2007 14:22:29 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] FW: HTL question To: "Dolores Townsend" , "Jeanine (CDC/CCID/NCZVED) Bartlett" , histonet@lists.utsouthwestern.edu Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C5@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii I disagree. An experienced pathologist will see and know fungus on an H&E. He/She does not need another stain, billed to the patient, for confirmation. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 12:35 PM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 14 Date: Wed, 11 Apr 2007 15:34:13 -0400 From: Philip Oshel Subject: RE: [Histonet] Re: Bone saw To: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Mid-mitten Michigan. The way this weather system is moving, you may yet see snow. (I like Canada, but I could do without the exports from Nunavut and the Northwest Territories.) And I only see the snow if I crawl up out of the dungeon where us EM trolls are generally kept. Phil >Hey Phil, where are you? I would love to see some snow.. rain here in >Hotlanta. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip >Oshel >Sent: Wednesday, April 11, 2007 1:48 PM >To: Histonet@Pathology.swmed.edu >Subject: RE: [Histonet] Re: Bone saw > >Rubber bracelets, H&E colored. >Enjoy the snow today! 2 years ago, I would've been on the 10th floor >of Animal Sciences, and I suspect unable to see the UW Hospital for >the flakes. > >Phll > >> >> >> >> >>I know what you mean! Here we can't get a tissue processor because the >>Breast Radiology folks "forgot" to order all of the required components >>for their digital scanner. Again, millions versus thousands. >> >> >> Maybe we all need to hire publicity consultants or agents to get >>the word out... :) >> >> >> >>Claire Ingles >> >>UW Hospital >> >>Madison WI >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >024C Brooks Hall >Central Michigan University >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message >may be privileged and is confidential information intended for the >use of the addressee listed above. If you are neither the intended >recipient nor the employee or agent responsible for delivering this >message to the intended recipient, you are hereby notified that any >disclosure, copying, distribution or the taking of any action in >reliance on the contents of this information is strictly prohibited. >If you have received this communication in error, please notify us >immediately by replying to the message and deleting it from your >computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 15 Date: Wed, 11 Apr 2007 12:48:57 -0700 (PDT) From: Akemi Allison-Tacha Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E To: "Horn, Hazel V" , Dolores Townsend , "Jeanine \(CDC/CCID/NCZVED\) Bartlett" , histonet@lists.utsouthwestern.edu Message-ID: <862289.72421.qm@web31315.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You have opened Pandora's Box!! One thing I have learned, once it's in writing, no turning back! --- "Horn, Hazel V" wrote: > I disagree. An experienced pathologist will see and > know fungus on an > H&E. He/She does not need another stain, billed to > the patient, for > confirmation. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dolores > Townsend > Sent: Wednesday, April 11, 2007 12:35 PM > To: Jeanine (CDC/CCID/NCZVED) Bartlett; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] FW: HTL question > > As for a lot of tests, the answer is a bit tricky > and confusing, but D > is right: although the pathologist can see something > that MIGHT be > fungus on the routine stain, (s)he will ask for a > special stain (GMS or > other) to confirm the presence of fungus. Hence, D. > Fungi are never > demonstrated with routine procedure. You will need > the special stain to > be sure. This is true for most special stains: the > pathologist sees > something on the routine stain, but it is not > conclusive, so special > stains are needed to confirm the diagnostic. > Dolores > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------------ > The information contained in this message may be > privileged and confidential > and protected from disclosure. If the reader of this > message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are hereby > notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this communication > in error, please notify > us immediately by replying to the message and > deleting it from your computer. > Thank you. > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Wed, 11 Apr 2007 15:02:23 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E To: "Akemi Allison-Tacha" , "Dolores Townsend" , "Jeanine (CDC/CCID/NCZVED) Bartlett" , histonet@lists.utsouthwestern.edu Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C8@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii Oops.... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Wednesday, April 11, 2007 2:49 PM To: Horn, Hazel V; Dolores Townsend; Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E You have opened Pandora's Box!! One thing I have learned, once it's in writing, no turning back! --- "Horn, Hazel V" wrote: > I disagree. An experienced pathologist will see and > know fungus on an > H&E. He/She does not need another stain, billed to > the patient, for > confirmation. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dolores > Townsend > Sent: Wednesday, April 11, 2007 12:35 PM > To: Jeanine (CDC/CCID/NCZVED) Bartlett; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] FW: HTL question > > As for a lot of tests, the answer is a bit tricky > and confusing, but D > is right: although the pathologist can see something > that MIGHT be > fungus on the routine stain, (s)he will ask for a > special stain (GMS or > other) to confirm the presence of fungus. Hence, D. > Fungi are never > demonstrated with routine procedure. You will need > the special stain to > be sure. This is true for most special stains: the > pathologist sees > something on the routine stain, but it is not > conclusive, so special > stains are needed to confirm the diagnostic. > Dolores > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------ ------ > The information contained in this message may be > privileged and confidential > and protected from disclosure. If the reader of this > message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are hereby > notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this communication > in error, please notify > us immediately by replying to the message and > deleting it from your computer. > Thank you. > ======================================================================== ====== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 17 Date: Wed, 11 Apr 2007 14:04:49 -0600 From: Gayle Callis Subject: [Histonet] Seasonal snap freezing noses and ears in Montana To: Philip Oshel , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070411134619.01b0bf58@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Ah, you guys have it WARM, so don't be wussies! It has been snowing here all week. Come visit Montana where our annual Easter Bunny snowstorm only dropped 8 inches heavy wet white UGH! this year (normal is ~ 2 ft!) Temperatures plunged to 20F - 25F with winds making the annual Easter egg hunt a ear and nose snap freezing experience. The front yard mini glacier should be taken out with Global Warming by end of week though. Springtime in the Rockies is sooo much fun at times. Gayle Callis From Frozen Reaches of Montana! At 01:34 PM 4/11/2007, you wrote: >Mid-mitten Michigan. The way this weather system is moving, you may yet >see snow. (I like Canada, but I could do without the exports from Nunavut >and the Northwest Territories.) > >And I only see the snow if I crawl up out of the dungeon where us EM >trolls are generally kept. >Phil > >>Hey Phil, where are you? I would love to see some snow.. rain here in >>Hotlanta. >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip >>Oshel >>Sent: Wednesday, April 11, 2007 1:48 PM >>To: Histonet@Pathology.swmed.edu >>Subject: RE: [Histonet] Re: Bone saw >> >>Rubber bracelets, H&E colored. >>Enjoy the snow today! 2 years ago, I would've been on the 10th floor >>of Animal Sciences, and I suspect unable to see the UW Hospital for >>the flakes. >> >>Phll ------------------------------ Message: 18 Date: Wed, 11 Apr 2007 16:08:45 -0400 From: Philip Oshel Subject: [Histonet] web resource for histology To: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Listers, I just came upon this site and thought I'd pass it on. Hope it's not repeating a well-known resource. http://courseweb.edteched.uottawa.ca/Medicine-histology/Default_En.htm Also available in French. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 ------------------------------ Message: 19 Date: Wed, 11 Apr 2007 15:12:54 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] Re: Bone saw To: Message-ID: Content-Type: text/plain; charset="us-ascii" While I don't wish to seem an old petarder, most of the several emails we have seen re bone saws have nothing to do with bone saws. How about decreasing the amount of interpersonal chatter that is being distributed to all on the Histonet so that we don't have to waste our time with opening and deleting all these? Thanks for your consideration Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, April 11, 2007 2:00 PM To: Philip Oshel; Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Re: Bone saw Yeah, Snow Brizzard in the middle of April...After coming back from 75 degree weather. I feel like like Dorothy clicking her heels.... THERE'S NO PLACE LIKE HOME!! THERE'S NO PLACE LIKE HOME!! Won't be long now. Akemi --- Philip Oshel wrote: > Rubber bracelets, H&E colored. > Enjoy the snow today! 2 years ago, I would've been > on the 10th floor > of Animal Sciences, and I suspect unable to see the > UW Hospital for > the flakes. > > Phll > > > > > > > > > > >I know what you mean! Here we can't get a tissue > processor because the > >Breast Radiology folks "forgot" to order all of the > required components > >for their digital scanner. Again, millions versus > thousands. > > > > > > Maybe we all need to hire publicity consultants > or agents to get > >the word out... :) > > > > > > > >Claire Ingles > > > >UW Hospital > > > >Madison WI > -- > Philip Oshel > Microscopy Facility Supervisor > Biology Department > 024C Brooks Hall > Central Michigan University > Mt. Pleasant, MI 48859 > voice: (989) 774-3576 > dept. fax: (989) 774-3462 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 11 Apr 2007 15:19:23 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] web resource for histology To: "Philip Oshel" , Histonet@Pathology.swmed.edu Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C9@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii I browsed this site a bit and it is good. I'll spend more time there when I can. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Wednesday, April 11, 2007 3:09 PM To: Histonet@Pathology.swmed.edu Subject: [Histonet] web resource for histology Listers, I just came upon this site and thought I'd pass it on. Hope it's not repeating a well-known resource. http://courseweb.edteched.uottawa.ca/Medicine-histology/Default_En.htm Also available in French. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 21 Date: Wed, 11 Apr 2007 18:11:53 -0400 From: "Richard Cartun" Subject: Re: [Histonet] When staining IHC's, are labs putting control tissue on every slide? To: , "James F. Happel" Message-ID: <461D24E902000077000053E6@gwmail.harthosp.org> Content-Type: text/plain; charset=US-ASCII No, our positive control tissues are placed on separate slides. We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Happel, James F." 04/10/07 4:29 PM >>> Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 22 Date: Wed, 11 Apr 2007 18:20:25 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" Subject: RE: [Histonet] FW: HTL question To: "Horn, Hazel V" , "Dolores Townsend" , Message-ID: Content-Type: text/plain; charset="UTF-8" Absolutely! Sometimes you need that special for confirmation but definteley not always. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wed 4/11/2007 3:22 PM To: Dolores Townsend; Bartlett, Jeanine (CDC/CCID/NCZVED); histonet@lists.utsouthwestern.edu Cc: Subject: RE: [Histonet] FW: HTL question I disagree. An experienced pathologist will see and know fungus on an H&E. He/She does not need another stain, billed to the patient, for confirmation. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Wednesday, April 11, 2007 12:35 PM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: HTL question As for a lot of tests, the answer is a bit tricky and confusing, but D is right: although the pathologist can see something that MIGHT be fungus on the routine stain, (s)he will ask for a special stain (GMS or other) to confirm the presence of fungus. Hence, D. Fungi are never demonstrated with routine procedure. You will need the special stain to be sure. This is true for most special stains: the pathologist sees something on the routine stain, but it is not conclusive, so special stains are needed to confirm the diagnostic. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 23 Date: Wed, 11 Apr 2007 18:21:59 -0400 From: "Richard Cartun" Subject: Re: [Histonet] FW: HTL question To: "Jeanine (CDC/CCID/NCZVED) Bartlett" , Message-ID: <461D274702000077000053EF@gwmail.harthosp.org> Content-Type: text/plain; charset=US-ASCII I would have picked "C" as well. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 04/11/07 1:23 PM >>> All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 41, Issue 17 **************************************** From jnocito <@t> satx.rr.com Wed Apr 11 21:02:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 11 21:02:19 2007 Subject: [Histonet] validation of IHC slides for Benchmark Stainer References: <7B5F5D9A00739F43A1819403EC7CF49103C3221C@thm-mail.thomaswv.org> Message-ID: <011201c77ca6$99245920$11614542@yourxhtr8hvc4p> I'm keeping the slides for 2 years and then discarding them. the paperwork we will keep for 10 years. We just validated the HPV HR & LR back in November and had our CAP inspection in January. The inspectors just glanced over them and didn't say anything one way or another. Just my 3 cents (might have to go to 4 cents if the price of gas keeps going up) JTT ----- Original Message ----- From: "Price, Tiffany" To: Sent: Wednesday, April 11, 2007 9:06 AM Subject: [Histonet] validation of IHC slides for Benchmark Stainer Could someone tell me if I need to keep all of the slides that I stained to optimize my IHC protocols, the slide for the final protocol I keep, or is a printed log signed off by the Pathologist sufficient for documentation? Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 11 21:05:13 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 11 21:05:19 2007 Subject: [Histonet] FW: HTL question Can diagnose fungus from H&E References: <862289.72421.qm@web31315.mail.mud.yahoo.com> Message-ID: <014001c77ca7$0348e500$11614542@yourxhtr8hvc4p> Hey, someone else is going to get flamed instead of me. YOU GO Hazel. I have the fire extinguisher ready for ya. JTT ----- Original Message ----- From: "Akemi Allison-Tacha" To: "Horn, Hazel V" ; "Dolores Townsend" ; "Jeanine (CDC/CCID/NCZVED) Bartlett" ; Sent: Wednesday, April 11, 2007 2:48 PM Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E > You have opened Pandora's Box!! > One thing I have learned, once it's in writing, no > turning back! > > --- "Horn, Hazel V" wrote: > >> I disagree. An experienced pathologist will see and >> know fungus on an >> H&E. He/She does not need another stain, billed to >> the patient, for >> confirmation. >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Dolores >> Townsend >> Sent: Wednesday, April 11, 2007 12:35 PM >> To: Jeanine (CDC/CCID/NCZVED) Bartlett; >> histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] FW: HTL question >> >> As for a lot of tests, the answer is a bit tricky >> and confusing, but D >> is right: although the pathologist can see something >> that MIGHT be >> fungus on the routine stain, (s)he will ask for a >> special stain (GMS or >> other) to confirm the presence of fungus. Hence, D. >> Fungi are never >> demonstrated with routine procedure. You will need >> the special stain to >> be sure. This is true for most special stains: the >> pathologist sees >> something on the routine stain, but it is not >> conclusive, so special >> stains are needed to confirm the diagnostic. >> Dolores >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > ------------------------------------------------------------------------------ >> The information contained in this message may be >> privileged and confidential >> and protected from disclosure. If the reader of this >> message is not the >> intended recipient, or an employee or agent >> responsible for delivering this >> message to the intended recipient, you are hereby >> notified that any >> dissemination, distribution or copying of this >> communication is strictly >> prohibited. If you have received this communication >> in error, please notify >> us immediately by replying to the message and >> deleting it from your computer. >> Thank you. >> > ============================================================================== >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Apr 11 23:17:26 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Apr 11 23:17:33 2007 Subject: [Histonet] IHC Message-ID: <041220070417.945.461DB2D60008A625000003B122070029539D09020704040A0105@comcast.net> Atoska, Don't know if you've been answered privately. Are you asking if anyone is staining FOR phage? Or are you asking about using (maxibodies, minibodies, etc) as IHC staining reagents that were derived from phage library panning and screening as an alternative to classical hybridoma antibodies? I might be able to help if no one else has written. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: Atoska Gentry > hello, is anyone doing any phage IHC staining, preferably on frozen > sections? One of our researchers is trying to come up with a suitable > staining protocol and I decided to inquire on blocking agents used > including recommended percentages. Thank you. Atoska > > -- > Atoska S. Gentry, B.S., HT(ASCP) > Research Assistant IV > Scott-Ritchey RSCH Center > College of Vet. Med > Auburn, AL 36849 > PH (334) 844-5579 > FAX (334) 844-5850 > email: gentras@vetmed.auburn.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Apr 11 23:42:37 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Apr 11 23:42:41 2007 Subject: [Histonet] California Society for Histotechnology Message-ID: The CSH annual symposium will be held in San Mateo, California from May 17 to May 20. San Mateo is about 10 minutes from the San Francisco airport. I have the program available as an attachment if you are interested. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From nyilmaz <@t> mersin.edu.tr Thu Apr 12 00:41:18 2007 From: nyilmaz <@t> mersin.edu.tr (Nejat) Date: Thu Apr 12 00:41:20 2007 Subject: [Histonet] Lapham's Stain Message-ID: <002c01c77cc5$321161f0$5201a8c0@NEJAT1> Dear Listers Does anybody know Lapham's stain method for nerve tissue. We couldn't find any source. Thanks in advance... Dr. Necat Yilmaz From TMcNemar <@t> lmhealth.org Thu Apr 12 04:56:35 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Apr 12 04:56:44 2007 Subject: [Histonet] When staining IHC's, are labs putting controltissue on every slide? In-Reply-To: <461D24E902000077000053E6@gwmail.harthosp.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F47D@lmhsmail.lmhealth.org> We put a positive control on every slide. We use the slides with the red box for most of them. The only ihc controls that we cut ahead are the H. Pylori. everything else we cut as needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, April 11, 2007 6:12 PM To: histonet@lists.utsouthwestern.edu; James F. Happel Subject: Re: [Histonet] When staining IHC's, are labs putting controltissue on every slide? No, our positive control tissues are placed on separate slides. We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Happel, James F." 04/10/07 4:29 PM >>> Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Apr 12 07:53:27 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Apr 12 07:53:32 2007 Subject: [Histonet] Lapham's Stain References: <002c01c77cc5$321161f0$5201a8c0@NEJAT1> Message-ID: <898D946569A27444B65667A49C074052D0E1CF@mailbe06.mc.vanderbilt.edu> Hello. Chuck Churukian of the University of Rochester Medical Center (Rochester, NY) used to include this procedure in his stain manual. I'm not sure if it's still in there, but you could contact him and find out. NSH should have his contact info or consult the website www.urmc.edu . I know it was still included in 1997 when I worked there, but I'm not sure about newer editions which are available for purchase. It is an excellent resource and unfortunately mine is out on loan right now or I'd just give you the procedure. Good Luck. Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 phone (615) 343-7089 fax TSH President NSH Quality Control Committee Chair ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Nejat Sent: Thu 4/12/2007 12:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Lapham's Stain Dear Listers Does anybody know Lapham's stain method for nerve tissue. We couldn't find any source. Thanks in advance... Dr. Necat Yilmaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Apr 12 07:57:58 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Apr 12 07:58:04 2007 Subject: [Histonet] When staining IHC's, are labs putting controltissue on every slide? In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F47D@lmhsmail.lmhealth.org> Message-ID: Tom, Don't you go through a lot of control tissue but cutting it new each time you need it? We cut our control slides ahead of time and those that are temperature sensitive we store at -20 degrees C with no problem. For instance, we generally cut our tonsil control 200 at a time. and store some at RT and some at -20. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, April 12, 2007 4:57 AM To: Richard Cartun; histonet@lists.utsouthwestern.edu; James F. Happel Subject: RE: [Histonet] When staining IHC's,are labs putting controltissue on every slide? We put a positive control on every slide. We use the slides with the red box for most of them. The only ihc controls that we cut ahead are the H. Pylori. everything else we cut as needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, April 11, 2007 6:12 PM To: histonet@lists.utsouthwestern.edu; James F. Happel Subject: Re: [Histonet] When staining IHC's, are labs putting controltissue on every slide? No, our positive control tissues are placed on separate slides. We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Happel, James F." 04/10/07 4:29 PM >>> Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tiffany.Price <@t> thomaswv.org Thu Apr 12 08:36:07 2007 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Thu Apr 12 08:36:18 2007 Subject: [Histonet] IHC validation Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C32226@thm-mail.thomaswv.org> -----Original Message----- I thought you would be interested in knowing the CAP response to my question about IHC slides for validation. Tiffany From: Mr. Edward J Gruber [mailto:egruber@cap.org] Sent: Wednesday, April 11, 2007 3:56 PM To: Price, Tiffany Subject: RE: Professional Practice Issues Good laboratory practice dictates that when validating IHC protocols, the slides should be retained for at least two years as part of the laboratory quality control system. Any of the slides that were used for the assessment should be saved as those were used to arrive at your finalized approach. The paper documentation records should be retained for the life of the instrumentation, technology or test system methodology. Thank you for your inquiry of the Laboratory Accreditation Program Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From mgdelaware <@t> comcast.net Thu Apr 12 08:48:53 2007 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Thu Apr 12 08:48:55 2007 Subject: [Histonet] Visionbiosystems Bond Message-ID: <000801c77d09$4ffe44f0$0711c847@D7XQNX91> Hello all: Anyone out there using the Bond? What are the advantages and disadvantages? Any hidden costs, etc.? Thanks in advance. Marian From TMcNemar <@t> lmhealth.org Thu Apr 12 08:53:31 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Apr 12 08:53:41 2007 Subject: [Histonet] When staining IHC's, are labs putting controltissue on every slide? In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F47E@lmhsmail.lmhealth.org> Yes, but I'm sure that our IHC volumne is no where near what your is. I have thought about cutting and keeping some tonsil since that is our most used control but it's also easy to come by so I haven't really felt the need. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Sebree Linda A. [mailto:LSebree@uwhealth.org] Sent: Thursday, April 12, 2007 8:58 AM To: Tom McNemar; Richard Cartun; histonet@lists.utsouthwestern.edu; James F. Happel Subject: RE: [Histonet] When staining IHC's,are labs putting controltissue on every slide? Tom, Don't you go through a lot of control tissue but cutting it new each time you need it? We cut our control slides ahead of time and those that are temperature sensitive we store at -20 degrees C with no problem. For instance, we generally cut our tonsil control 200 at a time. and store some at RT and some at -20. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, April 12, 2007 4:57 AM To: Richard Cartun; histonet@lists.utsouthwestern.edu; James F. Happel Subject: RE: [Histonet] When staining IHC's,are labs putting controltissue on every slide? We put a positive control on every slide. We use the slides with the red box for most of them. The only ihc controls that we cut ahead are the H. Pylori. everything else we cut as needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, April 11, 2007 6:12 PM To: histonet@lists.utsouthwestern.edu; James F. Happel Subject: Re: [Histonet] When staining IHC's, are labs putting controltissue on every slide? No, our positive control tissues are placed on separate slides. We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Happel, James F." 04/10/07 4:29 PM >>> Good Afternoon All, When staining IHC's, is anyone/everyone putting control tissue on every slide? Both + and - ? James -----Original Message----- From: Liz Beitman [mailto:LBeitman@cellmarque.com] Sent: Friday, March 23, 2007 12:19 PM To: Happel, James F. Subject: RE: [Histonet] Question about historical photographs Hi James, I just want to mention that we do have some posters of all the paintings that adorn our catalog that I can send to you. If you take a look in our catalog (volume 7), page 79, it will give you an idea of some of the posters. Some are rather interesting from different points of view of featured artists (Picasso, Klimt, and Michelangelo). They might fit in with your history presentation. If you find any of interest, I would look into either getting you an image or the actually poster. Please let me know. Thanks, Liz Beitman Cell Marque 1-800-665-7284 x 8981 lbeitman@cellmarque.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Thu Apr 12 09:45:20 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Apr 12 09:45:35 2007 Subject: [Histonet] Lapham's Stain Message-ID: <461E4600.40500@vetmed.auburn.edu> Dr. Necat, the Lapham's stain that I've used for myelin & glial fibers is in the 3rd edition of _AFIP Manual of Histologic Staining Methods,_ pp 204-205. It's an easy stain and yields beautiful results. Best wishes.Atoska _-- _ Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From awatanabe <@t> tgen.org Thu Apr 12 10:10:44 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Thu Apr 12 10:10:52 2007 Subject: [Histonet] Re: Vision Biosystems In-Reply-To: <20070412135648.0215420026D6@mr1.tgen.org> Message-ID: I use the BondMax for IHC staining. I love the reproducibility of the staining. I'm a research lab so the machine is 99% open for me. The reason it's not 100% open is you have to use at least one component of their detection kits for every run. I have switched to their kits exclusively except for some of the weird research antibodies and/or tissue I use. I have seen the ability to push titers out and use less antibody and reagents per run than most machines. It's very user friendly. The hidden costs come from the fact that you have to use their reagents for things like antigen retrieval, wash buffer and dewaxing, but these reagents are worth the money. I would recommend the machine for clinical or research work. Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From vazquezr <@t> ohsu.edu Thu Apr 12 11:55:12 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Apr 12 11:55:51 2007 Subject: [Histonet] Hello all Message-ID: Hello all Would anyone know where I could look for a troubleshooting guide for H & E staining? Thanks in advance Robyn OHSU From akemiat3377 <@t> yahoo.com Thu Apr 12 12:15:02 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 12 12:15:14 2007 Subject: [Histonet] Hello all In-Reply-To: Message-ID: <420966.4499.qm@web31306.mail.mud.yahoo.com> A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Apr 12 12:15:10 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 12 12:15:19 2007 Subject: [Histonet] Hello all In-Reply-To: Message-ID: <676225.38557.qm@web31301.mail.mud.yahoo.com> A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Apr 12 12:15:11 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 12 12:15:21 2007 Subject: [Histonet] Hello all In-Reply-To: Message-ID: <457793.87073.qm@web31311.mail.mud.yahoo.com> A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Apr 12 12:15:12 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 12 12:17:57 2007 Subject: [Histonet] Hello all In-Reply-To: Message-ID: <82803.73928.qm@web31302.mail.mud.yahoo.com> A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Apr 12 12:33:14 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 12 12:33:21 2007 Subject: [Histonet] Sorry-Internet problems Message-ID: <897651.79340.qm@web31308.mail.mud.yahoo.com> Sorry for the multiple H&E responses. My connection is messed-up. Akemi From splaza <@t> tylerpathology.com Thu Apr 12 12:38:23 2007 From: splaza <@t> tylerpathology.com (Susan Plaza) Date: Thu Apr 12 12:38:42 2007 Subject: [Histonet] Job Opening in East Texas Message-ID: <000201c77d29$5ef145f0$800aa8c0@domain.local> Full-time Histotechnologist/Histologic Technician position available at Pathology Associates of Tyler in scenic East Texas. Should be ASCP registered or registry-eligible. P.A.T. offers a highly competitive salary with an excellent benefits package. Interested applicants should forward their resume to: Pathology Associates of Tyler, 1726 South Beckham Ave., Tyler, TX 75701. Fax: (903)592-0555. Phone: (903)593-0481 or e-mail: sgibson@tylerpathology.com From lpwenk <@t> sbcglobal.net Thu Apr 12 19:03:26 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 12 19:04:35 2007 Subject: [Histonet] ASCP exam stats July-Dec. 2006 Message-ID: <000001c77d5f$2a738f50$a6bb2e4b@HPPav2> In case anyone wants the HT and HTL exam statistics for July-Dec 2006. If you want information on other categories or other years, go to: http://www.ascp.org/Certification/ForProgramDirectors/default.aspx Couple of notes first: - Range of scores is easiest question 100 to hardest question 999. Same with practical exam score numbers. - Minimul score of 400 degree of difficulty is required to pass each part. - Both the practical (PRAC) and the multiple choice questions (MCQ) must be passed, in order to pass the complete (COMP) exam. However, each candidates has 5 tries, so they may pass one part one year, and try again the next year - which is why the numbers don't match up. - NAACLS is the National Accrediting Agency for Clinical Laboratory Sciences. It is the agency that accredits HT and HTL programs. ASCP records the number of NAACLS students taking the exam for the first time. - The other category on the website is Total # Taking Exam. This would be NAACLS students taking it for the first time, repeating NAACLS students, OJT (on the job training) candidates taking it for the first time, and OJT candidates repeating the exam. - The "Non-NAACLS" category is one I extrapolated. It is the total # minus the NAACLS first timers. That leave the OJT first timers, OJT repeaters, and NAACLS repeaters (small number). HT MCQ - Mean = 432 - Range of Scores = 145-749 - Total # taking exam = 228 - Total pass = 134 = 59% - NAACLS students taking exam = 86 - NAACLS students passing = 72 = 86% - Non-NAACLS taking exam = 142 - Non-NAACLS passing = 62 = 44% HT PRAC - Mean = 543 - Range of Scores = 100-971 - Total # taking exam = 192 - Total pass = 166 = 57% - NAACLS students taking exam = 117 - NAACLS students passing = 109 = 93% - Non-NAACLS taking exam = 65 - Non-NAACLS passing = 57 = 88% HT COMP - Total # taking exam = 262 - Total pass = 189 = 72% - NAACLS students taking exam = 64 - NAACLS students passing = 62 = 93% - Non-NAACLS taking exam = 198 - Non-NAACLS passing = 127 = 64% First year certified = 1948 Total Certified to date = 20,087 - - - - - - HTL MCQ - Mean = 434 - Range of Scores = 162-681 - Total # taking exam = 56 - Total pass = 39 = 70% - NAACLS students taking exam = 7 - NAACLS students passing = 7 = 100% - Non-NAACLS taking exam = 49 - Non-NAACLS passing = 32 = 65% HTL PRAC - Mean = 550 - Range of Scores = 325 - 736 - Total # taking exam = 43 - Total pass = 39 = 91% - NAACLS students taking exam = 8 - NAACLS students passing = 8 = 100% - Non-NAACLS taking exam = 35 - Non-NAACLS passing = 31 = 86% HTL COMP - Total # taking exam = 46 - Total pass = 40 = 87% - NAACLS students taking exam = 8 - NAACLS students passing = 8 = 100% - Non-NAACLS taking exam = 38 - Non-NAACLS passing = 32% = 84% First year certified = 1980 Total Certified to date = 2,372 - - - - - - Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, mI 48073 From detmar <@t> mshri.on.ca Thu Apr 12 20:46:31 2007 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Apr 12 20:46:49 2007 Subject: [Histonet] rodent smooth muscle marker Message-ID: Hi all. I am asking this question on behalf of a colleague. She wishes to measure rat uterine smooth muscle cell volume over gestation and would like to have a marker that would delinate the smooth muscle plasma membrane. I have looked at some of my Masson trichrome-stained mouse placental sections and this *might* be helpful for her; however, I was thinking one of you might know of a good lectin or antibody marker. Like I said, she works on rat tissue, but I wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers, too. Thanks a bunch! Jacqui Detmar Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON M5G 1X5 From liz <@t> premierlab.com Thu Apr 12 21:12:44 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Apr 12 20:59:34 2007 Subject: [Histonet] rodent smooth muscle marker In-Reply-To: Message-ID: <000001c77d71$39a3a1b0$0d00a8c0@domain.Premier> What about alpha smooth muscle actin, most anti-human SMA antibodies will cross react with rodent. If she is planning on both rat and mouse tissues then I would choose a rabbit antibody to smooth muscle actin. The one that we use works in both rat and mouse, its from abcam ab5694. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui Detmar Sent: Thursday, April 12, 2007 7:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rodent smooth muscle marker Hi all. I am asking this question on behalf of a colleague. She wishes to measure rat uterine smooth muscle cell volume over gestation and would like to have a marker that would delinate the smooth muscle plasma membrane. I have looked at some of my Masson trichrome-stained mouse placental sections and this *might* be helpful for her; however, I was thinking one of you might know of a good lectin or antibody marker. Like I said, she works on rat tissue, but I wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers, too. Thanks a bunch! Jacqui Detmar Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON M5G 1X5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From anh2006 <@t> med.cornell.edu Thu Apr 12 21:31:45 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Apr 12 21:31:56 2007 Subject: [Histonet] rodent smooth muscle marker In-Reply-To: <000001c77d71$39a3a1b0$0d00a8c0@domain.Premier> References: <000001c77d71$39a3a1b0$0d00a8c0@domain.Premier> Message-ID: Along those same lines mouse anti-SMAalpha clone 1A4 works very well for SMC in mouse and human. To avoid having to use a MOM kit I use the EPOS version from DAKO which is VERY pricey but worth every penny for the gorgeous and clean result in mouse tissues (it can also be diluted and doesn't need to be used neat as recommended by manufacturer). Andrea >What about alpha smooth muscle actin, most anti-human SMA antibodies will >cross react with rodent. If she is planning on both rat and mouse tissues >then I would choose a rabbit antibody to smooth muscle actin. The one that >we use works in both rat and mouse, its from abcam ab5694. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, CO 80308 >phone (303) 735-5001 >fax (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: > >Premier Laboratory, LLC >University of Colorado at Boulder >MCDB, Room A3B40 >Boulder, CO 80309 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui >Detmar >Sent: Thursday, April 12, 2007 7:47 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] rodent smooth muscle marker > >Hi all. I am asking this question on behalf of a colleague. She wishes to >measure rat uterine smooth muscle cell volume over gestation and would like >to have a marker that would delinate the smooth muscle plasma membrane. I >have looked at some of my Masson trichrome-stained mouse placental sections >and this *might* be helpful for her; however, I was thinking one of you >might know of a good lectin or antibody marker. Like I said, she works on >rat tissue, but I wouldn't mind receiving some advice on mouse uterine >smooth muscle cell markers, too. > >Thanks a bunch! > >Jacqui Detmar >Samuel Lunenfeld Research Institute, room 876 >Mount Sinai Hospital >600 University Avenue >Toronto, ON M5G 1X5 > -- From yvan_lindekens <@t> yahoo.com Fri Apr 13 04:17:47 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Fri Apr 13 04:17:54 2007 Subject: [Histonet] plant anatomy - sectioning problems Message-ID: <292946.36182.qm@web30909.mail.mud.yahoo.com> Hi all, I'm trying to cut some plant samples but I have lots of problems with the samples containing even the smallest amount of wood (f.e. young white deadnettle stems - Lamium album L.). A thickness of 15 micron is the thinnest I can get, without the ribbon scattering longitudaly after only a few sections. Whiping the blade with a cloth moisted with some xylene solves the problem, but only for another 2 or 3 sections... I use a Leica-Jung Autocut 1140 and Feather S/R/N 35 blades. Cutting at veeeeeeryyyyy looooow speed gives slightly better results but still not good enough! When sectioned on a sliding microtome(Reichert-Jung Hn-40, Feather S/R/N 35 blades, declination angle +/- 130?), the sections are okay, but I would like to use a rotary. Samples were fixed in AFA, processed by hand using an ETOH/IPA/xylene protocol. On the other hand I processed some samples using a tert. butyl alcohol schedule. There's hardly any difference between the two regarding sectioning properties... By the way: is there something as a "standard thickness" for plantanatomical sections? Thanks in advance for every suggestion! Yvan. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From TownsendD <@t> childrensdayton.org Fri Apr 13 08:45:55 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Apr 13 08:46:19 2007 Subject: [Histonet] Demonstration of H. Pylori Message-ID: Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend From JWEEMS <@t> sjha.org Fri Apr 13 08:49:50 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Apr 13 08:50:27 2007 Subject: [Histonet] Demonstration of H. Pylori In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3BD1@sjhaexc02.sjha.org> We do Genta - actually a modified Genta. We have the Artisan - so we do a Warthin Starry and add the rest off line. No IHC. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 13 09:00:12 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Apr 13 09:00:22 2007 Subject: [Histonet] Demonstration of H. Pylori Message-ID: <407F05A128805F4C879A33DBA32E618E20C2BD@TRFT-EX01.xRothGen.nhs.uk> I am replying on behalf of my technologists to say that their pathologist likes ipox, though usually does with an H & E. He asks for it when there is an appearance which suggests H. pylori should be there but can't see it, or when he sees organisms but they are not typical shape. (H. pylori occasionally has a coccal form). One of the girls did a project comparing 6 or 7 stains and the best was ipox,as one would expect. The next best was Giema, in that it gave the best staining against background light staining, most consistently. Silver stains were pretty hopeless. Tol. Blue easily overstained, and others were too laborious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: 13 April 2007 14:46 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Ferrigon <@t> sanofi-aventis.com Fri Apr 13 09:02:31 2007 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Fri Apr 13 09:02:37 2007 Subject: [Histonet] Microwaves for use in antigen retrieval. Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D1E197B@ALPW31.f2.enterprise> Hi I would like to purchase a microwave to use in our ICC lab for antigen retrieval, can anyone recommend a reliable laboratory microwave???( at present we have a old household Sanyo model but would like to buy a new one where we can accurately measure the internal temperature). Thanks Susan From doug <@t> ppspath.com Fri Apr 13 10:08:22 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Apr 13 09:06:42 2007 Subject: [Histonet] Demonstration of H. Pylori In-Reply-To: Message-ID: We do IHC. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Apr 13 09:14:51 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Apr 13 09:13:57 2007 Subject: [Histonet] rodent smooth muscle marker In-Reply-To: References: Message-ID: <461F905B.3030509@umdnj.edu> Periodic acid-Shiff (PAS) will outline the smooth muscle cells by staining the cell coat external to the plasma membrane. Geoff Jacqui Detmar wrote: >Hi all. I am asking this question on behalf of a colleague. She wishes to measure rat uterine smooth muscle cell volume over gestation and would like to have a marker that would delinate the smooth muscle plasma membrane. I have looked at some of my Masson trichrome-stained mouse placental sections and this *might* be helpful for her; however, I was thinking one of you might know of a good lectin or antibody marker. Like I said, she works on rat tissue, but I wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers, too. > >Thanks a bunch! > >Jacqui Detmar >Samuel Lunenfeld Research Institute, room 876 >Mount Sinai Hospital >600 University Avenue >Toronto, ON M5G 1X5 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From burch007 <@t> mc.duke.edu Fri Apr 13 09:21:14 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Apr 13 09:21:21 2007 Subject: [Histonet] Demonstration of H. Pylori In-Reply-To: Message-ID: Hello Delores and everyone else, We use IHC for the demonstration of H pylori. I don't get caught up in the argument of IHC vs. special stains. If our doctors like IHC, that's fine with me. Job security says it all. I use Dako's rabbit polyclonal antibody at 1:100, pretreat with Brigati's stable pepsin, and detect with a biotin/streptavidin system. I like AEC, but sometimes use DAB as the chromogen. After taking a couple years off from Histonet.... I'm back Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Dolores Townsend" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 08:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From EWURDAK <@t> CSBSJU.EDU Fri Apr 13 09:29:21 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Fri Apr 13 09:29:28 2007 Subject: [Histonet] plant anatomy - sectioning problems In-Reply-To: <292946.36182.qm@web30909.mail.mud.yahoo.com> Message-ID: Hi Yvan, The procedure we followed is listed below. We had pretty good results as far as sectioning went. We are staining with safranin and fast green or hematoxylin and safranin. Neither comes out as bright as I would like to see it. I would welcome any suggestions you have for staining. Liz Processing Plant Tissues for Paraffin Sections On January 5 and 6, 2007 various plant organs were collected in the SJU Greenhouse. They were fixed in FAA and kept in this solution until February 13 and 14, 2007. The composition of FAA is as follows: 95% ethanol 50 cc glacial acetic acid 5 cc formalin 10 cc distilled water 35 cc Wiley, R. L. 1971 Microtechniques: A Laboratory Guide, The Macmillan Company, New York Dehydration and clearing was carried out according to the following schedule: 1. Jar #1: 30:50:20 water:alcohol:tert-butyl-alcohol 2. Jar #2: 15:50:35 water:alcohol:tert-butyl-alcohol for 1 hour. 3. Jar #3: 25:75 alcohol:tert-butyl-alcohol for 1 hour. 4. Jar #4: 100% Tert-butyl-alcohol for 1 hour. 5. Jar #5: 100% Tert-butyl-alcohol for 1 hour. 6. Jar #6: 100% Tert-butyl-alcohol + paraffin chips, ON at 45oC. 7. Jar #7: Pure paraffin 30 min, 60oC under vacuum 8. Jar #8: Pure paraffin in 60oC oven 1hr. 9. Jar #9: Third change of pure paraffin in 60oC oven 1hr. 10. Embed. This schedule is a modified version of the protocol we followed in 1997. I also consulted Grey, P. 1964 Handbook of Basic Microtechnique 3rd. ed, McGraw-Hill Book Company, New York. Ruzin, S. E. 1999 Plant Microtechnique and Microscopy, Oxford University Press, New York On 4/13/07 4:17 AM, "yvan lindekens" wrote: > Hi all, > > I'm trying to cut some plant samples but I have lots > of problems with the samples containing even the > smallest amount of wood (f.e. young white deadnettle > stems - Lamium album L.). > > A thickness of 15 micron is the thinnest I can get, > without the ribbon scattering longitudaly after only a > few sections. Whiping the blade with a cloth moisted > with some xylene solves the problem, but only for > another 2 or 3 sections... I use a Leica-Jung Autocut > 1140 and Feather S/R/N 35 blades. > > Cutting at veeeeeeryyyyy looooow speed gives slightly > better results but still not good enough! > > When sectioned on a sliding microtome(Reichert-Jung > Hn-40, Feather S/R/N 35 blades, declination angle +/- > 130?), the sections are okay, but I would like to use > a rotary. > > Samples were fixed in AFA, processed by hand using an > ETOH/IPA/xylene protocol. On the other hand I > processed some samples using a tert. butyl alcohol > schedule. There's hardly any difference between the > two regarding sectioning properties... > > By the way: is there something as a "standard > thickness" for plantanatomical sections? > > Thanks in advance for every suggestion! > > Yvan. > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHosp.org Fri Apr 13 09:37:21 2007 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Fri Apr 13 09:38:29 2007 Subject: [Histonet] Demonstration of H. Pylori References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E0F43@fhosxchmb006.ADVENTISTCORP.NET> We do a Giemsa stain. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dolores Townsend Sent: Fri 4/13/2007 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From TownsendD <@t> childrensdayton.org Fri Apr 13 09:46:37 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Apr 13 09:47:14 2007 Subject: [Histonet] H. Pylori IHC Message-ID: One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores From sbreeden <@t> nmda.nmsu.edu Fri Apr 13 09:48:01 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Apr 13 09:48:09 2007 Subject: [Histonet] Fluorescein Question Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F43FD@nmdamailsvr.nmda.ad.nmsu.edu> A current project is cutting slides to check for fluorescein expression. The first time I did this, I was asked to air-dry the slides and mount with aqueous medium; as you can expect, the coverage was "iffy" at best with lifting of the coverslip, etc. Is there any reason I could not dry these in a 60 degree oven as usual, then deparaffinize and coverslip out of xylene? Would this process compromise the results? Help? Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Fri Apr 13 09:50:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 13 09:50:17 2007 Subject: [Histonet] Demonstration of H. Pylori In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47802E0F43@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <874731.20789.qm@web61224.mail.yahoo.com> We do modified Steiner Ren? J. "Bonner, Janet" wrote: We do a Giemsa stain. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dolores Townsend Sent: Fri 4/13/2007 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From HornHV <@t> archildrens.org Fri Apr 13 09:51:04 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Apr 13 09:51:25 2007 Subject: [Histonet] H. Pylori IHC In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70CF@EMAIL.archildrens.org> We do IHC only if requested by the pathologist. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori IHC One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From burch007 <@t> mc.duke.edu Fri Apr 13 09:58:09 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Apr 13 09:58:17 2007 Subject: [Histonet] H. Pylori IHC In-Reply-To: Message-ID: It depends on the clinical history. Some GI bx's are accessioned and H pylori is prospectively ordered. Other IHC requests come in as a direct order from the attending pathologist or resident in training. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Dolores Townsend" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 09:46 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] H. Pylori IHC One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Apr 13 10:16:24 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Apr 13 10:16:17 2007 Subject: CoverslippingRe: [Histonet] Fluorescein Question In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F43FD@nmdamailsvr.nmda.ad .nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F43FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6.0.0.22.1.20070413090850.01b5f278@gemini.msu.montana.edu> Sara, Try it, but keep sections in the dark to avoid photobleaching. Fluorescein should survive deparaffinization and then coverslipping. Putting in an extra xylene to ensure good paraffin removal before putting a coverglass on may be of help. We have some who do that here but they are requesting thick sections ~ 10 um or so. I generally just air dry at RT in a dark place. If you want to know the effects of temperature on FITC, contact Molecular Probes technical services, they will know about this also. However, if the tissues are fixed with formalin, you may be battling autofluorescence. At 08:48 AM 4/13/2007, you wrote: >A current project is cutting slides to check for fluorescein expression. >The first time I did this, I was asked to air-dry the slides and mount >with aqueous medium; as you can expect, the coverage was "iffy" at best >with lifting of the coverslip, etc. Is there any reason I could not dry >these in a 60 degree oven as usual, then deparaffinize and coverslip out >of xylene? Would this process compromise the results? Help? Thanks! > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From pmarcum <@t> vet.upenn.edu Fri Apr 13 10:24:03 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Apr 13 10:24:11 2007 Subject: [Histonet] Need a Fixative Formula Message-ID: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> Does anyone have a formula for the old original Lillie's Fixative? Someone asked me for it and I can't find it in any of my books here. Thanks in Advance!! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From stern <@t> ipmc.cnrs.fr Fri Apr 13 10:26:13 2007 From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern) Date: Fri Apr 13 10:25:28 2007 Subject: [Histonet] Fixatives for IHF Message-ID: <461FA115.2080502@ipmc.cnrs.fr> Hi all, I am working on mouse lung, and have come across a small problem. Basically I embed the lungs in OCT/sucrose with snap freezing in Isopentane and then postfix. Initially I was postfixing in pure acetone but there staining with certain antibodies was not working We are trying to detect: DAPI, Smooth muscle actin (directly labeled PE), CD4 (Directly labeled Alexa 647), Thy1.1 (FITC coupled + GARabitt Alexa 488) and Thy1.2 (Biotinylated + Stv Alexa 594) Now the conflict: When I postfix with 100% EtOH I get a very nice staining with Thy1.1 and CD4 but have not managed to see the Thy1.1 and the SMA looks terrible When I postfix with either Acetone or PAF 1% the SMA look lovely but the CD4 staining is very dull. I have not managed yet to detect the Stv Alexa 594 signal yet. How can I get the best of both worlds? A very nice and well demarcated SMA staining (as with PAF of Acetone) with a nice CD4 staining (as with EtOH). Any input will be welcomed. Alejandro -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From benoit.delatour <@t> u-psud.fr Fri Apr 13 10:38:50 2007 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Beno=EEt_Delatour?=) Date: Fri Apr 13 10:35:14 2007 Subject: [Histonet] fluoro-jade Message-ID: <461FA40A.7080604@u-psud.fr> Dear Lisa, I performed FJ staining on formaldehyde-fixed brain tissue (frozen rat/mice 40?m sections on Superfrost+ slides) using the protocol detailed in [Schmued LC, Hopkins KJ (2000) Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration. Brain Res 874: 123-130]. It works fine, staining all degenerating material with minimal background. I can send you the paper if you do not have it. Good luck! Benoit -- Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Mobile 06 82 99 10 92 Email benoit.delatour@u-psud.fr Web http://www.namc.u-psud.fr From plucas <@t> biopath.org Fri Apr 13 10:40:39 2007 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Apr 13 10:40:44 2007 Subject: [Histonet] Proper Decalcification Message-ID: <20070413154039.D1DF032B0@centaur.cnchost.com> I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group From jimr0712 <@t> comcast.net Fri Apr 13 10:40:42 2007 From: jimr0712 <@t> comcast.net (jimr0712@comcast.net) Date: Fri Apr 13 10:40:46 2007 Subject: [Histonet] HSV 1&2 staining Message-ID: <041320071540.27129.461FA47A000E13DA000069F92200745672CDCEC9CF9D030706@comcast.net> Could someone share the current methodology that they are using on the DAKO Autostainer Plus for HSV 1 & 2 staining ? Thanks. -- Jim Robinson, M.S., HTL/HT (ASCP) Director of Laboratory Operations Orizon Diagnostics, LLC 102 Chestnut Avenue Westmont, Illinois 60559 jrobinson@orizondiagnostics.com 630-321-1506 (fax) 630-230-6325 From Barbara_Lentz <@t> dahlchase.com Fri Apr 13 10:40:55 2007 From: Barbara_Lentz <@t> dahlchase.com (Barbara Lentz) Date: Fri Apr 13 10:43:16 2007 Subject: [Histonet] Demonstration of H. Pylori Message-ID: All Gastric Biopsies are stained up front with "Little Quicker" (a modified Giemsa) and given out with the H&E's. Depending on the pathologist, an IHC may be ordered subsequently. >>> "Dolores Townsend" 04/13/07 09:45AM >>> Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 13 10:47:20 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Apr 13 10:47:25 2007 Subject: [Histonet] FW: HTL question Message-ID: <407F05A128805F4C879A33DBA32E618E20C2C3@TRFT-EX01.xRothGen.nhs.uk> C is the best answer. D is plain wrong. Moreover, it is ridiculous. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 11 April 2007 18:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: HTL question All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Apr 13 10:50:23 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Apr 13 10:50:28 2007 Subject: [Histonet] FW: HTL question References: <407F05A128805F4C879A33DBA32E618E20C2C3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Hah! Thanks! -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, April 13, 2007 11:47 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: HTL question C is the best answer. D is plain wrong. Moreover, it is ridiculous. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 11 April 2007 18:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: HTL question All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation B. are removed in the > routine staining process C. stain variably with the H&E procedure D. > are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Apr 13 11:00:15 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Apr 13 11:00:20 2007 Subject: [Histonet] Fluorescein Follow-up Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4401@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to those that responded to my fluorescein question (is fluorescein removed by xylene?). I had two conflicting responses and that brings up a very logical (I should have seen this before...) question: if fluorescein is removed by xylene, am I not "dead in the water" right out of the box although I do use Sub-X on the processor (but xylene in the stain line). Is THAT a factor (the Sub-X) as it's a XYL substitute? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From HDOWNS <@t> PARTNERS.ORG Fri Apr 13 11:02:50 2007 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Fri Apr 13 11:07:26 2007 Subject: [Histonet] 11. plant anatomy - sectioning problems (yvan lindekens) In-Reply-To: <20070413151750.D570844828F@phsmgmx3.partners.org> References: <20070413151750.D570844828F@phsmgmx3.partners.org> Message-ID: If possible and you can use resins, try Spurr's. It was specifically designed to use with plants and vegetables, and you can cut as thin as 1 micron. The resin is easy to use, is less toxic that epon, and has great penetration. Heather Downs BS Nerve Injury Unit Mass General Hospital Boston Mass, 02114 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, April 13, 2007 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 41, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Hello all (Akemi Allison-Tacha) 2. Re: Hello all (Akemi Allison-Tacha) 3. Re: Hello all (Akemi Allison-Tacha) 4. Re: Hello all (Akemi Allison-Tacha) 5. Sorry-Internet problems (Akemi Allison-Tacha) 6. Job Opening in East Texas (Susan Plaza) 7. ASCP exam stats July-Dec. 2006 (Lee & Peggy Wenk) 8. rodent smooth muscle marker (Jacqui Detmar) 9. RE: rodent smooth muscle marker (Liz Chlipala) 10. RE: rodent smooth muscle marker (Andrea Hooper) 11. plant anatomy - sectioning problems (yvan lindekens) 12. Demonstration of H. Pylori (Dolores Townsend) 13. RE: Demonstration of H. Pylori (Weems, Joyce) 14. RE: Demonstration of H. Pylori (Marshall Terry Dr, Consultant Histopathologist) 15. Microwaves for use in antigen retrieval. (Susan.Ferrigon@sanofi-aventis.com) 16. RE: Demonstration of H. Pylori (Douglas D Deltour) 17. Re: rodent smooth muscle marker (Geoff McAuliffe) 18. Re: Demonstration of H. Pylori (James L Burchette) 19. Re: plant anatomy - sectioning problems (Wurdak, Elizabeth) 20. RE: Demonstration of H. Pylori (Bonner, Janet) 21. H. Pylori IHC (Dolores Townsend) 22. Fluorescein Question (Breeden, Sara) 23. RE: Demonstration of H. Pylori (Rene J Buesa) 24. RE: H. Pylori IHC (Horn, Hazel V) 25. Re: H. Pylori IHC (James L Burchette) 26. CoverslippingRe: [Histonet] Fluorescein Question (Gayle Callis) ---------------------------------------------------------------------- Message: 1 Date: Thu, 12 Apr 2007 10:15:02 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Hello all To: Robyn Vazquez , Histonet@lists.utsouthwestern.edu Message-ID: <420966.4499.qm@web31306.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Thu, 12 Apr 2007 10:15:10 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Hello all To: Robyn Vazquez , Histonet@lists.utsouthwestern.edu Message-ID: <676225.38557.qm@web31301.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Thu, 12 Apr 2007 10:15:11 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Hello all To: Robyn Vazquez , Histonet@lists.utsouthwestern.edu Message-ID: <457793.87073.qm@web31311.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Thu, 12 Apr 2007 10:15:12 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Hello all To: Robyn Vazquez , Histonet@lists.utsouthwestern.edu Message-ID: <82803.73928.qm@web31302.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A couple of weeks ago there was quite a bit of discussion on that subject. If you go to the histonet archives and pull up H&E artifacts, it will give you the book info with contacts. Akemi Allison-Tacha --- Robyn Vazquez wrote: > Hello all > > Would anyone know where I could look for a > troubleshooting guide for H > & E staining? > > Thanks in advance > > Robyn > OHSU > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Thu, 12 Apr 2007 10:33:14 -0700 (PDT) From: Akemi Allison-Tacha Subject: [Histonet] Sorry-Internet problems To: histonet@lists.utsouthwestern.edu Message-ID: <897651.79340.qm@web31308.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Sorry for the multiple H&E responses. My connection is messed-up. Akemi ------------------------------ Message: 6 Date: Thu, 12 Apr 2007 12:38:23 -0500 From: "Susan Plaza" Subject: [Histonet] Job Opening in East Texas To: , "':'" Message-ID: <000201c77d29$5ef145f0$800aa8c0@domain.local> Content-Type: text/plain; charset="us-ascii" Full-time Histotechnologist/Histologic Technician position available at Pathology Associates of Tyler in scenic East Texas. Should be ASCP registered or registry-eligible. P.A.T. offers a highly competitive salary with an excellent benefits package. Interested applicants should forward their resume to: Pathology Associates of Tyler, 1726 South Beckham Ave., Tyler, TX 75701. Fax: (903)592-0555. Phone: (903)593-0481 or e-mail: sgibson@tylerpathology.com ------------------------------ Message: 7 Date: Thu, 12 Apr 2007 20:03:26 -0400 From: "Lee & Peggy Wenk" Subject: [Histonet] ASCP exam stats July-Dec. 2006 To: Message-ID: <000001c77d5f$2a738f50$a6bb2e4b@HPPav2> Content-Type: text/plain; charset="us-ascii" In case anyone wants the HT and HTL exam statistics for July-Dec 2006. If you want information on other categories or other years, go to: http://www.ascp.org/Certification/ForProgramDirectors/default.aspx Couple of notes first: - Range of scores is easiest question 100 to hardest question 999. Same with practical exam score numbers. - Minimul score of 400 degree of difficulty is required to pass each part. - Both the practical (PRAC) and the multiple choice questions (MCQ) must be passed, in order to pass the complete (COMP) exam. However, each candidates has 5 tries, so they may pass one part one year, and try again the next year - which is why the numbers don't match up. - NAACLS is the National Accrediting Agency for Clinical Laboratory Sciences. It is the agency that accredits HT and HTL programs. ASCP records the number of NAACLS students taking the exam for the first time. - The other category on the website is Total # Taking Exam. This would be NAACLS students taking it for the first time, repeating NAACLS students, OJT (on the job training) candidates taking it for the first time, and OJT candidates repeating the exam. - The "Non-NAACLS" category is one I extrapolated. It is the total # minus the NAACLS first timers. That leave the OJT first timers, OJT repeaters, and NAACLS repeaters (small number). HT MCQ - Mean = 432 - Range of Scores = 145-749 - Total # taking exam = 228 - Total pass = 134 = 59% - NAACLS students taking exam = 86 - NAACLS students passing = 72 = 86% - Non-NAACLS taking exam = 142 - Non-NAACLS passing = 62 = 44% HT PRAC - Mean = 543 - Range of Scores = 100-971 - Total # taking exam = 192 - Total pass = 166 = 57% - NAACLS students taking exam = 117 - NAACLS students passing = 109 = 93% - Non-NAACLS taking exam = 65 - Non-NAACLS passing = 57 = 88% HT COMP - Total # taking exam = 262 - Total pass = 189 = 72% - NAACLS students taking exam = 64 - NAACLS students passing = 62 = 93% - Non-NAACLS taking exam = 198 - Non-NAACLS passing = 127 = 64% First year certified = 1948 Total Certified to date = 20,087 - - - - - - HTL MCQ - Mean = 434 - Range of Scores = 162-681 - Total # taking exam = 56 - Total pass = 39 = 70% - NAACLS students taking exam = 7 - NAACLS students passing = 7 = 100% - Non-NAACLS taking exam = 49 - Non-NAACLS passing = 32 = 65% HTL PRAC - Mean = 550 - Range of Scores = 325 - 736 - Total # taking exam = 43 - Total pass = 39 = 91% - NAACLS students taking exam = 8 - NAACLS students passing = 8 = 100% - Non-NAACLS taking exam = 35 - Non-NAACLS passing = 31 = 86% HTL COMP - Total # taking exam = 46 - Total pass = 40 = 87% - NAACLS students taking exam = 8 - NAACLS students passing = 8 = 100% - Non-NAACLS taking exam = 38 - Non-NAACLS passing = 32% = 84% First year certified = 1980 Total Certified to date = 2,372 - - - - - - Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, mI 48073 ------------------------------ Message: 8 Date: Thu, 12 Apr 2007 21:46:31 -0400 From: "Jacqui Detmar" Subject: [Histonet] rodent smooth muscle marker To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all. I am asking this question on behalf of a colleague. She wishes to measure rat uterine smooth muscle cell volume over gestation and would like to have a marker that would delinate the smooth muscle plasma membrane. I have looked at some of my Masson trichrome-stained mouse placental sections and this *might* be helpful for her; however, I was thinking one of you might know of a good lectin or antibody marker. Like I said, she works on rat tissue, but I wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers, too. Thanks a bunch! Jacqui Detmar Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON M5G 1X5 ------------------------------ Message: 9 Date: Thu, 12 Apr 2007 20:12:44 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] rodent smooth muscle marker To: "'Jacqui Detmar'" , Message-ID: <000001c77d71$39a3a1b0$0d00a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" What about alpha smooth muscle actin, most anti-human SMA antibodies will cross react with rodent. If she is planning on both rat and mouse tissues then I would choose a rabbit antibody to smooth muscle actin. The one that we use works in both rat and mouse, its from abcam ab5694. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui Detmar Sent: Thursday, April 12, 2007 7:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rodent smooth muscle marker Hi all. I am asking this question on behalf of a colleague. She wishes to measure rat uterine smooth muscle cell volume over gestation and would like to have a marker that would delinate the smooth muscle plasma membrane. I have looked at some of my Masson trichrome-stained mouse placental sections and this *might* be helpful for her; however, I was thinking one of you might know of a good lectin or antibody marker. Like I said, she works on rat tissue, but I wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers, too. Thanks a bunch! Jacqui Detmar Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON M5G 1X5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com ------------------------------ Message: 10 Date: Thu, 12 Apr 2007 22:31:45 -0400 From: "Andrea Hooper" Subject: RE: [Histonet] rodent smooth muscle marker To: Liz Chlipala , Histonet Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Along those same lines mouse anti-SMAalpha clone 1A4 works very well for SMC in mouse and human. To avoid having to use a MOM kit I use the EPOS version from DAKO which is VERY pricey but worth every penny for the gorgeous and clean result in mouse tissues (it can also be diluted and doesn't need to be used neat as recommended by manufacturer). Andrea >What about alpha smooth muscle actin, most anti-human SMA antibodies will >cross react with rodent. If she is planning on both rat and mouse tissues >then I would choose a rabbit antibody to smooth muscle actin. The one that >we use works in both rat and mouse, its from abcam ab5694. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, CO 80308 >phone (303) 735-5001 >fax (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: > >Premier Laboratory, LLC >University of Colorado at Boulder >MCDB, Room A3B40 >Boulder, CO 80309 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui >Detmar >Sent: Thursday, April 12, 2007 7:47 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] rodent smooth muscle marker > >Hi all. I am asking this question on behalf of a colleague. She wishes to >measure rat uterine smooth muscle cell volume over gestation and would like >to have a marker that would delinate the smooth muscle plasma membrane. I >have looked at some of my Masson trichrome-stained mouse placental sections >and this *might* be helpful for her; however, I was thinking one of you >might know of a good lectin or antibody marker. Like I said, she works on >rat tissue, but I wouldn't mind receiving some advice on mouse uterine >smooth muscle cell markers, too. > >Thanks a bunch! > >Jacqui Detmar >Samuel Lunenfeld Research Institute, room 876 >Mount Sinai Hospital >600 University Avenue >Toronto, ON M5G 1X5 > -- ------------------------------ Message: 11 Date: Fri, 13 Apr 2007 02:17:47 -0700 (PDT) From: yvan lindekens Subject: [Histonet] plant anatomy - sectioning problems To: histonet@lists.utsouthwestern.edu Message-ID: <292946.36182.qm@web30909.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, I'm trying to cut some plant samples but I have lots of problems with the samples containing even the smallest amount of wood (f.e. young white deadnettle stems - Lamium album L.). A thickness of 15 micron is the thinnest I can get, without the ribbon scattering longitudaly after only a few sections. Whiping the blade with a cloth moisted with some xylene solves the problem, but only for another 2 or 3 sections... I use a Leica-Jung Autocut 1140 and Feather S/R/N 35 blades. Cutting at veeeeeeryyyyy looooow speed gives slightly better results but still not good enough! When sectioned on a sliding microtome(Reichert-Jung Hn-40, Feather S/R/N 35 blades, declination angle +/- 130?), the sections are okay, but I would like to use a rotary. Samples were fixed in AFA, processed by hand using an ETOH/IPA/xylene protocol. On the other hand I processed some samples using a tert. butyl alcohol schedule. There's hardly any difference between the two regarding sectioning properties... By the way: is there something as a "standard thickness" for plantanatomical sections? Thanks in advance for every suggestion! Yvan. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 12 Date: Fri, 13 Apr 2007 09:45:55 -0400 From: "Dolores Townsend" Subject: [Histonet] Demonstration of H. Pylori To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend ------------------------------ Message: 13 Date: Fri, 13 Apr 2007 09:49:50 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Demonstration of H. Pylori To: "Dolores Townsend" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3BD1@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" We do Genta - actually a modified Genta. We have the Artisan - so we do a Warthin Starry and add the rest off line. No IHC. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 14 Date: Fri, 13 Apr 2007 15:00:12 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Demonstration of H. Pylori To: "Dolores Townsend" , Message-ID: <407F05A128805F4C879A33DBA32E618E20C2BD@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="us-ascii" I am replying on behalf of my technologists to say that their pathologist likes ipox, though usually does with an H & E. He asks for it when there is an appearance which suggests H. pylori should be there but can't see it, or when he sees organisms but they are not typical shape. (H. pylori occasionally has a coccal form). One of the girls did a project comparing 6 or 7 stains and the best was ipox,as one would expect. The next best was Giema, in that it gave the best staining against background light staining, most consistently. Silver stains were pretty hopeless. Tol. Blue easily overstained, and others were too laborious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: 13 April 2007 14:46 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 13 Apr 2007 15:02:31 +0100 From: Subject: [Histonet] Microwaves for use in antigen retrieval. To: Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D1E197B@ALPW31.f2.enterprise> Content-Type: text/plain; charset="us-ascii" Hi I would like to purchase a microwave to use in our ICC lab for antigen retrieval, can anyone recommend a reliable laboratory microwave???( at present we have a old household Sanyo model but would like to buy a new one where we can accurately measure the internal temperature). Thanks Susan ------------------------------ Message: 16 Date: Fri, 13 Apr 2007 10:08:22 -0500 From: "Douglas D Deltour" Subject: RE: [Histonet] Demonstration of H. Pylori To: "'Dolores Townsend'" , Message-ID: Content-Type: text/plain; charset="us-ascii" We do IHC. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 13 Apr 2007 10:14:51 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] rodent smooth muscle marker To: Jacqui Detmar Cc: histonet@lists.utsouthwestern.edu Message-ID: <461F905B.3030509@umdnj.edu> Content-Type: text/plain; format=flowed; charset=us-ascii Periodic acid-Shiff (PAS) will outline the smooth muscle cells by staining the cell coat external to the plasma membrane. Geoff Jacqui Detmar wrote: >Hi all. I am asking this question on behalf of a colleague. She wishes to measure rat uterine smooth muscle cell volume over gestation and would like to have a marker that would delinate the smooth muscle plasma membrane. I have looked at some of my Masson trichrome-stained mouse placental sections and this *might* be helpful for her; however, I was thinking one of you might know of a good lectin or antibody marker. Like I said, she works on rat tissue, but I wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers, too. > >Thanks a bunch! > >Jacqui Detmar >Samuel Lunenfeld Research Institute, room 876 >Mount Sinai Hospital >600 University Avenue >Toronto, ON M5G 1X5 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 18 Date: Fri, 13 Apr 2007 09:21:14 -0500 From: James L Burchette Subject: Re: [Histonet] Demonstration of H. Pylori To: "Dolores Townsend" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello Delores and everyone else, We use IHC for the demonstration of H pylori. I don't get caught up in the argument of IHC vs. special stains. If our doctors like IHC, that's fine with me. Job security says it all. I use Dako's rabbit polyclonal antibody at 1:100, pretreat with Brigati's stable pepsin, and detect with a biotin/streptavidin system. I like AEC, but sometimes use DAB as the chromogen. After taking a couple years off from Histonet.... I'm back Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Dolores Townsend" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 08:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 13 Apr 2007 09:29:21 -0500 From: "Wurdak, Elizabeth" Subject: Re: [Histonet] plant anatomy - sectioning problems To: yvan lindekens , Histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hi Yvan, The procedure we followed is listed below. We had pretty good results as far as sectioning went. We are staining with safranin and fast green or hematoxylin and safranin. Neither comes out as bright as I would like to see it. I would welcome any suggestions you have for staining. Liz Processing Plant Tissues for Paraffin Sections On January 5 and 6, 2007 various plant organs were collected in the SJU Greenhouse. They were fixed in FAA and kept in this solution until February 13 and 14, 2007. The composition of FAA is as follows: 95% ethanol 50 cc glacial acetic acid 5 cc formalin 10 cc distilled water 35 cc Wiley, R. L. 1971 Microtechniques: A Laboratory Guide, The Macmillan Company, New York Dehydration and clearing was carried out according to the following schedule: 1. Jar #1: 30:50:20 water:alcohol:tert-butyl-alcohol 2. Jar #2: 15:50:35 water:alcohol:tert-butyl-alcohol for 1 hour. 3. Jar #3: 25:75 alcohol:tert-butyl-alcohol for 1 hour. 4. Jar #4: 100% Tert-butyl-alcohol for 1 hour. 5. Jar #5: 100% Tert-butyl-alcohol for 1 hour. 6. Jar #6: 100% Tert-butyl-alcohol + paraffin chips, ON at 45oC. 7. Jar #7: Pure paraffin 30 min, 60oC under vacuum 8. Jar #8: Pure paraffin in 60oC oven 1hr. 9. Jar #9: Third change of pure paraffin in 60oC oven 1hr. 10. Embed. This schedule is a modified version of the protocol we followed in 1997. I also consulted Grey, P. 1964 Handbook of Basic Microtechnique 3rd. ed, McGraw-Hill Book Company, New York. Ruzin, S. E. 1999 Plant Microtechnique and Microscopy, Oxford University Press, New York On 4/13/07 4:17 AM, "yvan lindekens" wrote: > Hi all, > > I'm trying to cut some plant samples but I have lots > of problems with the samples containing even the > smallest amount of wood (f.e. young white deadnettle > stems - Lamium album L.). > > A thickness of 15 micron is the thinnest I can get, > without the ribbon scattering longitudaly after only a > few sections. Whiping the blade with a cloth moisted > with some xylene solves the problem, but only for > another 2 or 3 sections... I use a Leica-Jung Autocut > 1140 and Feather S/R/N 35 blades. > > Cutting at veeeeeeryyyyy looooow speed gives slightly > better results but still not good enough! > > When sectioned on a sliding microtome(Reichert-Jung > Hn-40, Feather S/R/N 35 blades, declination angle +/- > 130?), the sections are okay, but I would like to use > a rotary. > > Samples were fixed in AFA, processed by hand using an > ETOH/IPA/xylene protocol. On the other hand I > processed some samples using a tert. butyl alcohol > schedule. There's hardly any difference between the > two regarding sectioning properties... > > By the way: is there something as a "standard > thickness" for plantanatomical sections? > > Thanks in advance for every suggestion! > > Yvan. > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 13 Apr 2007 10:37:21 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] Demonstration of H. Pylori To: "Dolores Townsend" , histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E0F43@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 We do a Giemsa stain. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dolores Townsend Sent: Fri 4/13/2007 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 21 Date: Fri, 13 Apr 2007 10:46:37 -0400 From: "Dolores Townsend" Subject: [Histonet] H. Pylori IHC To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores ------------------------------ Message: 22 Date: Fri, 13 Apr 2007 08:48:01 -0600 From: "Breeden, Sara" Subject: [Histonet] Fluorescein Question To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F43FD@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" A current project is cutting slides to check for fluorescein expression. The first time I did this, I was asked to air-dry the slides and mount with aqueous medium; as you can expect, the coverage was "iffy" at best with lifting of the coverslip, etc. Is there any reason I could not dry these in a 60 degree oven as usual, then deparaffinize and coverslip out of xylene? Would this process compromise the results? Help? Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 23 Date: Fri, 13 Apr 2007 07:50:05 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Demonstration of H. Pylori To: "Bonner, Janet" , Dolores Townsend , histonet@lists.utsouthwestern.edu Message-ID: <874731.20789.qm@web61224.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We do modified Steiner Ren? J. "Bonner, Janet" wrote: We do a Giemsa stain. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dolores Townsend Sent: Fri 4/13/2007 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Demonstration of H. Pylori Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 24 Date: Fri, 13 Apr 2007 09:51:04 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] H. Pylori IHC To: "Dolores Townsend" , histonet@lists.utsouthwestern.edu Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70CF@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii We do IHC only if requested by the pathologist. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori IHC One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 25 Date: Fri, 13 Apr 2007 09:58:09 -0500 From: James L Burchette Subject: Re: [Histonet] H. Pylori IHC To: "Dolores Townsend" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" It depends on the clinical history. Some GI bx's are accessioned and H pylori is prospectively ordered. Other IHC requests come in as a direct order from the attending pathologist or resident in training. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Dolores Townsend" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 09:46 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] H. Pylori IHC One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Fri, 13 Apr 2007 09:16:24 -0600 From: Gayle Callis Subject: CoverslippingRe: [Histonet] Fluorescein Question To: "Breeden, Sara" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070413090850.01b5f278@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Sara, Try it, but keep sections in the dark to avoid photobleaching. Fluorescein should survive deparaffinization and then coverslipping. Putting in an extra xylene to ensure good paraffin removal before putting a coverglass on may be of help. We have some who do that here but they are requesting thick sections ~ 10 um or so. I generally just air dry at RT in a dark place. If you want to know the effects of temperature on FITC, contact Molecular Probes technical services, they will know about this also. However, if the tissues are fixed with formalin, you may be battling autofluorescence. At 08:48 AM 4/13/2007, you wrote: >A current project is cutting slides to check for fluorescein expression. >The first time I did this, I was asked to air-dry the slides and mount >with aqueous medium; as you can expect, the coverage was "iffy" at best >with lifting of the coverslip, etc. Is there any reason I could not dry >these in a 60 degree oven as usual, then deparaffinize and coverslip out >of xylene? Would this process compromise the results? Help? Thanks! > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 41, Issue 20 **************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From rjbuesa <@t> yahoo.com Fri Apr 13 11:09:59 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 13 11:10:07 2007 Subject: [Histonet] Demonstration of H. Pylori In-Reply-To: Message-ID: <883936.71443.qm@web61217.mail.yahoo.com> We used modified Steiner for H. pylori Ren? J. Barbara Lentz wrote: All Gastric Biopsies are stained up front with "Little Quicker" (a modified Giemsa) and given out with the H&E's. Depending on the pathologist, an IHC may be ordered subsequently. >>> "Dolores Townsend" 04/13/07 09:45AM >>> Hello, Histonetters I am taking a census: what stain do you/your pathologists prefer to demonstrate H. Pylori? How many of you do IHC? How many do special stains, and if so, which one? I do a Steiner here, which is time consuming, even in the microwave, and seems to give variable results in staining intensity, so I'd like your opinion as to what stain you prefer. Thank you, Dolores Townsend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From gcallis <@t> montana.edu Fri Apr 13 11:17:15 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Apr 13 11:17:05 2007 Subject: [Histonet] Fluorescein Follow-up In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4401@nmdamailsvr.nmda.ad .nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F4401@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6.0.0.22.1.20070413101425.01b7b9b8@gemini.msu.montana.edu> We have one researcher who does this and is seeing the FITC after paraffin processing. We actually have one xylene, and one Clearite 3 on our processor. We deparaffinize in Clearite 3, with an extra change to ensure good paraffin removal. She is seeing FITC signal, although she asks for thick sections 10 um or more for confocal work. I don't know what Sub-X is? Some kind of xylene substitute. At 10:00 AM 4/13/2007, you wrote: >Thanks to those that responded to my fluorescein question (is >fluorescein removed by xylene?). I had two conflicting responses and >that brings up a very logical (I should have seen this before...) >question: if fluorescein is removed by xylene, am I not "dead in the >water" right out of the box although I do use Sub-X on the processor >(but xylene in the stain line). Is THAT a factor (the Sub-X) as it's a >XYL substitute? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From burch007 <@t> mc.duke.edu Fri Apr 13 11:23:00 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Apr 13 11:23:13 2007 Subject: [Histonet] HSV 1&2 staining In-Reply-To: <041320071540.27129.461FA47A000E13DA000069F92200745672CDCEC9CF9D030706@comcast.net> Message-ID: Mr. Robinson: I prepare an antibody cocktail of HSV I & II. No pretreatment for FFPE tissues. 30-45 minute primary, 20 minute HRP labeled Envision Plus, standard DAB (DAB+ not needed). HSV will work just as well with a biotin/streptavidin system. Rather straight forward, no tricks or hoops to jump through. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" jimr0712@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 10:40 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] HSV 1&2 staining Could someone share the current methodology that they are using on the DAKO Autostainer Plus for HSV 1 & 2 staining ? Thanks. -- Jim Robinson, M.S., HTL/HT (ASCP) Director of Laboratory Operations Orizon Diagnostics, LLC 102 Chestnut Avenue Westmont, Illinois 60559 jrobinson@orizondiagnostics.com 630-321-1506 (fax) 630-230-6325 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Fri Apr 13 11:27:28 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Apr 13 11:27:32 2007 Subject: [Histonet] Need a Fixative Formula References: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> Message-ID: <003901c77de8$a08d2dc0$6500a8c0@mainbox> Pam, Which one? Lillie published formulas for a number of different fixatives, these range from AAF to Zenker to the buffered sublimate series such as B4&B5 and even an alcoholic lead nitrate formalin! Let me know, maybe I can help. Regards, Bryan ----- Original Message ----- From: "Pamela Marcum" To: Sent: Friday, April 13, 2007 11:24 AM Subject: [Histonet] Need a Fixative Formula > > > Does anyone have a formula for the old original Lillie's Fixative? > Someone asked me for it and I can't find it in any of my books here. > Thanks in Advance!! > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AGrobe2555 <@t> aol.com Fri Apr 13 11:31:17 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Apr 13 11:31:26 2007 Subject: [Histonet] Re:Fluorescein Question Message-ID: Why not use a hardening fluorescence mounting medium like Fluorsave (Calbiochem/EMD Biosciences)? This would save time and eliminate the possibility of signal loss.................... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From rsrichmond <@t> aol.com Fri Apr 13 12:08:33 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Fri Apr 13 12:08:47 2007 Subject: [Histonet] Re: Demonstration of H. Pylori In-Reply-To: <200704131117.129461f9eef2b2@rly-mc05.mail.aol.com> References: <200704131117.129461f9eef2b2@rly-mc05.mail.aol.com> Message-ID: <8C94C05802A8FF8-E10-C443@WEBMAIL-RB08.sysops.aol.com> I have experience with two techniques for Helicobacter, blue dye (Diff-Quik II, Giemsa, toluidine blue), and immunohistochemistry. My experience is that the dye method often requires oil immersion examination (slightly time consuming), where IHC slides can be examined under much lower magnification. This difference doesn't matter if I have three gastric biopsies a week, but it matters a lot if I have ten in a day, plus fifty other cases to sign out. Many laboratories do the stain - whatever stain they do - on every gastric biopsy specimen. Others only do the stain only if it's specifically requested, or if neutrophilic infiltration (chronic active gastritis) is seen in the H & E sections. A current discussion going on on PATHO-L suggests that regulators are starting to prohibit doing IHC on every gastric biopsy specimen, and you should be following this point closely. I've often been told that real men can see Helicobacter in the H & E slides, but I can't reliably - maybe it's my 68 year old eyes. It's simpler to a quick stain with Diff-Quik II (or one of its generic equivalents) or with toluidine blue, rather than doing a full Giemsa stain. A frequently ought-to-be-asked question: does Helicobacter heilmannii (the bug formerly known as Gastrospirillum hominis) mark with the H. pylori antibody? Answer (according to Japanese sources - H. heilmannii is more common in Japan than in the rest of the world) is that it definitely does mark. (I've seen H. heilmannii once in my life, and that was good many years ago.) Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From dpahisto <@t> yahoo.com Fri Apr 13 12:41:15 2007 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Fri Apr 13 12:41:19 2007 Subject: [Histonet] Derm IHC Message-ID: <888256.81950.qm@web33410.mail.mud.yahoo.com> Currently we do not do IHC at our lab. My manager asked me "If we sign a Dermatology account" what should we offer the physician in the way of IHC? S-100, HMB-45? What is the standard these days? Also, what is the best way to process a small volume? (By hand, auto-stainer ??) Any comments and advice would be appreciated, Cindy DuBois Integrated Pathology --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From cmiller <@t> physlab.com Fri Apr 13 13:32:43 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Apr 13 13:32:50 2007 Subject: [Histonet] Need a Fixative Formula In-Reply-To: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> References: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> Message-ID: <000001c77dfa$22122920$db01a8c0@plab.local> This is our lilies we use it for our fatty tissues and love it. Stock solution; 200 ml glacial acetic acid 800 ml 95% alcohol Working solution; 1 part stock to 3 parts 10% neutral buffered formalin. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Friday, April 13, 2007 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need a Fixative Formula Does anyone have a formula for the old original Lillie's Fixative? Someone asked me for it and I can't find it in any of my books here. Thanks in Advance!! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Fri Apr 13 13:38:16 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Apr 13 13:39:30 2007 Subject: [Histonet] Proper Decalcification Message-ID: <1271778907.20070413223816@mail.ru> Paula: I use now "Weight loss, weight gain" method. It is easily and quickly for routine surgical purposes. Need some simple instruments: the scales, pencil, filter paper... and few seconds for one object. Earlier I used oxalate test, but it requires the half an hour (all reagents I prepared by itself). Thanks to Gayle Callis for advice. Maxim Peshkov Russia, Taganrog. From gcallis <@t> montana.edu Fri Apr 13 13:50:11 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Apr 13 13:49:59 2007 Subject: [Histonet] Spurrs? Re: plant anatomy - sectioning problems In-Reply-To: References: <20070413151750.D570844828F@phsmgmx3.partners.org> Message-ID: <6.0.0.22.1.20070413124646.01b8f8f8@gemini.msu.montana.edu> I had heard that Spurrs was discontinued. Hopefully there is a substitute and contacting Energy Beam Sciences may be one way to find out. Can anyone enlighten us on this? :02 AM 4/13/2007, you wrote: > If possible and you can use resins, try Spurr's. It was specifically > designed >to use with plants and vegetables, and you can cut as thin as 1 micron. The >resin is easy to use, is less toxic that epon, and has great penetration. > >Heather Downs BS >Nerve Injury Unit >Mass General Hospital >Boston Mass, 02114 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From rsrichmond <@t> aol.com Fri Apr 13 13:50:40 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Fri Apr 13 13:50:57 2007 Subject: [Histonet] Re: Fixative Formula In-Reply-To: <200704131300.113461fb73e174@rly-mc03.mail.aol.com> References: <200704131300.113461fb73e174@rly-mc03.mail.aol.com> Message-ID: <8C94C13C494B80E-E10-C922@WEBMAIL-RB08.sysops.aol.com> Pamela Marcum at the U of PA vet school down in mushroom country asks: Does anyone have a formula for the old original Lillie's Fixative? Someone asked me for it and I can't find it in any of my books here. Thanks in Advance!! There is no such thing. Ralph D. Lillie invented a great many fixatives. Ordinary neutral buffered formalin (buffered to neutrality with phosphate) was his invention. A formalin-ethanol-acetic acid mixture is also attributed to him by some. But the term "Lillie's fixative" should be avoided. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From LSebree <@t> uwhealth.org Fri Apr 13 14:01:22 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Apr 13 14:01:29 2007 Subject: [Histonet] Job available in Madison, WI Message-ID: Dear Histonet, The following is a job description I am posting for a friend: Histology Laboratory Technician Mirus Bio Corporation As a histology laboratory technician at Mirus this person will be an integral member of the research team. Duties Include: Harvesting tissues samples Processing frozen and paraffin embedded sections Maintaining accurate specimen logs and detailed laboratory notebooks Interfacing and working with scientist from other disciplines as the histology expert Qualification Requirements: Extensive histology experience (ASCP or ASVP certification) in tissue processing and staining Knowledge of immunohistochemistry a plus To apply, please send a cover letter and resume to Mirus Bio Corporation at one of the following addresses: Employment@mirusbio.com Mirus Bio Corporation Attn: Human Resources 505 S. Rosa Rd #104 Madison, WI 53719-1267 Fax: 608-441-2849 Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From bhewlett <@t> cogeco.ca Fri Apr 13 14:03:05 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Apr 13 14:03:48 2007 Subject: [Histonet] Need a Fixative Formula References: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> <000001c77dfa$22122920$db01a8c0@plab.local> Message-ID: <007a01c77dfe$6e5248c0$6500a8c0@mainbox> Cheri, Just what is the published reference for this wonderful elixir??? Bryan ----- Original Message ----- From: "Cheri Miller" To: "'Pamela Marcum'" ; Sent: Friday, April 13, 2007 2:32 PM Subject: RE: [Histonet] Need a Fixative Formula > > This is our lilies we use it for our fatty tissues and love it. > > > Stock solution; 200 ml glacial acetic acid > 800 ml 95% alcohol > > Working solution; 1 part stock to 3 parts 10% neutral buffered formalin. > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela > Marcum > Sent: Friday, April 13, 2007 10:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Need a Fixative Formula > > > > Does anyone have a formula for the old original Lillie's > Fixative? Someone asked me for it and I can't find it in any of my > books here. Thanks in Advance!! > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From oshel1pe <@t> cmich.edu Fri Apr 13 14:18:33 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Apr 13 14:18:42 2007 Subject: [Histonet] Spurrs? Re: plant anatomy - sectioning problems In-Reply-To: <6.0.0.22.1.20070413124646.01b8f8f8@gemini.msu.montana.edu> References: <20070413151750.D570844828F@phsmgmx3.partners.org> <6.0.0.22.1.20070413124646.01b8f8f8@gemini.msu.montana.edu> Message-ID: Spurr's as such has not been discontinued, but one of its components has. Which is good, it's my favorite resin for both plants and animals. ERL 4206, VCD, is no longer available, and ERL 4221 has been substituted. 4221 is Cycloaliphatic Epoxide Resin. The suppliers were (may still be) claiming that 4221 is a straight 1:1 substitute for 4206, but it isn't. Making Spurr's with the new component is covered in: E. Ann Ellis Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations Microscopy Today, July 2006 14(4):32 ff I can a PDF of the article to anyone who wants it. Phil >I had heard that Spurrs was discontinued. Hopefully there is a >substitute and contacting Energy Beam Sciences may be one way to >find out. Can anyone enlighten us on this? > >:02 AM 4/13/2007, you wrote: >> If possible and you can use resins, try Spurr's. It was >>specifically designed >>to use with plants and vegetables, and you can cut as thin as 1 micron. The >>resin is easy to use, is less toxic that epon, and has great penetration. >> >>Heather Downs BS >>Nerve Injury Unit >>Mass General Hospital >>Boston Mass, 02114 > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Technical Editor, Microscopy Today Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 fax: (989) 774-3462 From CIngles <@t> uwhealth.org Fri Apr 13 15:19:21 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Apr 13 15:19:26 2007 Subject: [Histonet] Derm IHC References: <888256.81950.qm@web33410.mail.mud.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120025@uwhis-xchng3.uwhis.hosp.wisc.edu> I think it depends on what diseases you are wanting to screen for. I have just started running stains for Melanoma. So far I have done HMB-45, S-100, Mart-1, and a Melanoma cocktail (Mart-1 & Tyrosinase). There are probably others for SCC, BCC, etc. I only run slides on frozen sections since I am in a MOHS lab. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Fri 4/13/2007 12:41 PM To: Histonet Subject: [Histonet] Derm IHC Currently we do not do IHC at our lab. My manager asked me "If we sign a Dermatology account" what should we offer the physician in the way of IHC? S-100, HMB-45? What is the standard these days? Also, what is the best way to process a small volume? (By hand, auto-stainer ??) Any comments and advice would be appreciated, Cindy DuBois Integrated Pathology --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From soofias2 <@t> yahoo.com Fri Apr 13 16:32:50 2007 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Fri Apr 13 16:32:53 2007 Subject: [Histonet] Derm IHC In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120025@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <108020.86368.qm@web39510.mail.mud.yahoo.com> In my opinion for small volumes manual staining is the cheapest. There are surface markers available for CTCL, CBCL, differentiation of dermatitis over mycosis fungoides. We carry more than 30 antibodies in our dermatology lab for immunophenotyping on frozen skin sections. Soofia Siddiqui Molecular Diagnostics lab UW Hospital and Clinics Madison WI --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From jnocito <@t> satx.rr.com Fri Apr 13 18:20:53 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Apr 13 18:20:57 2007 Subject: [Histonet] H. Pylori IHC References: Message-ID: <00cb01c77e22$623a1530$11614542@yourxhtr8hvc4p> we do H. pylori on any bx from the stomach (antrum, cardia, pyloris, etc) as well as GE junction bx's with a HX of hiatal hernia. ----- Original Message ----- From: "Dolores Townsend" To: Sent: Friday, April 13, 2007 9:46 AM Subject: [Histonet] H. Pylori IHC > One more question for those who do IHC: do you do these routinely on all > gastric biopsies, or only as requested by your pathologist? > Thanks, > Dolores > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Apr 13 18:22:25 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Apr 13 18:22:26 2007 Subject: [Histonet] Demonstration of H. Pylori References: Message-ID: <00f001c77e22$992f24e0$11614542@yourxhtr8hvc4p> we do IHC all he time unless they didn't work and then we'll do a rapid modified Giemsa Jo Da To ----- Original Message ----- From: "Dolores Townsend" To: Sent: Friday, April 13, 2007 8:45 AM Subject: [Histonet] Demonstration of H. Pylori > Hello, Histonetters > I am taking a census: what stain do you/your pathologists prefer to > demonstrate H. Pylori? > How many of you do IHC? > How many do special stains, and if so, which one? > I do a Steiner here, which is time consuming, even in the microwave, > and seems to give variable results in staining intensity, so I'd like > your opinion as to what stain you prefer. > Thank you, > Dolores Townsend > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdhisto <@t> yahoo.com Fri Apr 13 19:32:18 2007 From: jdhisto <@t> yahoo.com (Jonathan De La Rosa) Date: Fri Apr 13 19:32:23 2007 Subject: [Histonet] GASTRIC BX'S Message-ID: <849441.92282.qm@web36208.mail.mud.yahoo.com> SHOULD IT NOT DEPEND ON THE PATHOLOGIST AND/OR THE ANATOMIC PATH LAB'S SUGGESTION TOWARDS THE H.P IHC TESTING IF ITS NOT A LAB WHERE ITS ROUTINE TO MAKE ALL OF THE SUGGESTED IHC TESTING DONE ON ALL OF GASTRO ENTEROLOGY SPECIMENS DUE TO PATIENT BILLING. CAN THIS NOT AMOUNT TO BILLING THAT IS NOT REQUIRED? JD AUSTIN, TX Jonathan N. De La Rosa HT Austin Gastroenterology Anatomic Path. Laboratory 210-412-5837 jdelarosa@austingastro.com JDhisto@yahoo.com From jdhisto <@t> yahoo.com Fri Apr 13 19:36:03 2007 From: jdhisto <@t> yahoo.com (Jonathan De La Rosa) Date: Fri Apr 13 19:42:50 2007 Subject: [Histonet] (no subject) Message-ID: <20070414003603.18513.qmail@web36207.mail.mud.yahoo.com> RAPID MODIFIED GIEMSA? A SHORT CUT DQ STAIN! WHY NOT DO THE "MODIFIED GIEMSA" ON A ROUTINE BASES BEFORE THE HIGHER COST IHC IS PERFORMED? JD From JMacDonald <@t> mtsac.edu Sat Apr 14 00:03:44 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Apr 14 00:03:51 2007 Subject: [Histonet] FW: HTL question In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2C3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: There is more than one answer wrong in the study guide. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 08:47 AM To "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , cc Subject RE: [Histonet] FW: HTL question C is the best answer. D is plain wrong. Moreover, it is ridiculous. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 11 April 2007 18:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: HTL question All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Sat Apr 14 00:07:17 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Apr 14 00:07:22 2007 Subject: [Histonet] plant anatomy - sectioning problems In-Reply-To: Message-ID: We also have problems with the light green, so we add extra acetic acid to the working solution and get much better results. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Wurdak, Elizabeth" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 07:29 AM To yvan lindekens , Histonet cc Subject Re: [Histonet] plant anatomy - sectioning problems Hi Yvan, The procedure we followed is listed below. We had pretty good results as far as sectioning went. We are staining with safranin and fast green or hematoxylin and safranin. Neither comes out as bright as I would like to see it. I would welcome any suggestions you have for staining. Liz Processing Plant Tissues for Paraffin Sections On January 5 and 6, 2007 various plant organs were collected in the SJU Greenhouse. They were fixed in FAA and kept in this solution until February 13 and 14, 2007. The composition of FAA is as follows: 95% ethanol 50 cc glacial acetic acid 5 cc formalin 10 cc distilled water 35 cc Wiley, R. L. 1971 Microtechniques: A Laboratory Guide, The Macmillan Company, New York Dehydration and clearing was carried out according to the following schedule: 1. Jar #1: 30:50:20 water:alcohol:tert-butyl-alcohol 2. Jar #2: 15:50:35 water:alcohol:tert-butyl-alcohol for 1 hour. 3. Jar #3: 25:75 alcohol:tert-butyl-alcohol for 1 hour. 4. Jar #4: 100% Tert-butyl-alcohol for 1 hour. 5. Jar #5: 100% Tert-butyl-alcohol for 1 hour. 6. Jar #6: 100% Tert-butyl-alcohol + paraffin chips, ON at 45oC. 7. Jar #7: Pure paraffin 30 min, 60oC under vacuum 8. Jar #8: Pure paraffin in 60oC oven 1hr. 9. Jar #9: Third change of pure paraffin in 60oC oven 1hr. 10. Embed. This schedule is a modified version of the protocol we followed in 1997. I also consulted Grey, P. 1964 Handbook of Basic Microtechnique 3rd. ed, McGraw-Hill Book Company, New York. Ruzin, S. E. 1999 Plant Microtechnique and Microscopy, Oxford University Press, New York On 4/13/07 4:17 AM, "yvan lindekens" wrote: > Hi all, > > I'm trying to cut some plant samples but I have lots > of problems with the samples containing even the > smallest amount of wood (f.e. young white deadnettle > stems - Lamium album L.). > > A thickness of 15 micron is the thinnest I can get, > without the ribbon scattering longitudaly after only a > few sections. Whiping the blade with a cloth moisted > with some xylene solves the problem, but only for > another 2 or 3 sections... I use a Leica-Jung Autocut > 1140 and Feather S/R/N 35 blades. > > Cutting at veeeeeeryyyyy looooow speed gives slightly > better results but still not good enough! > > When sectioned on a sliding microtome(Reichert-Jung > Hn-40, Feather S/R/N 35 blades, declination angle +/- > 130?), the sections are okay, but I would like to use > a rotary. > > Samples were fixed in AFA, processed by hand using an > ETOH/IPA/xylene protocol. On the other hand I > processed some samples using a tert. butyl alcohol > schedule. There's hardly any difference between the > two regarding sectioning properties... > > By the way: is there something as a "standard > thickness" for plantanatomical sections? > > Thanks in advance for every suggestion! > > Yvan. > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michelle.Perrins <@t> uct.ac.za Sat Apr 14 04:34:44 2007 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Sat Apr 14 04:34:59 2007 Subject: [Histonet] ZN stain Message-ID: <4620BC54.A704.0070.0@uct.ac.za> Hi Does anyone have a situation when staining for mycobacterium tuberculosis with the ZN stain, get negative results in the tissue sampled but the control is positive, in cases where the patient/deceased is known to have had TB. I work with autopsy material and have this situation when we know the person had TB but is negative in the test. Anyone have any other stain to demonstrate TB bacilli or information on this issue. Many thanks Michelle Forensic Pathology Services Western Cape South Africa From rjbuesa <@t> yahoo.com Sat Apr 14 11:00:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 14 11:00:44 2007 Subject: [Histonet] Safety survey-summary Message-ID: <440474.97221.qm@web61211.mail.yahoo.com> The safety survey has received a very good response, with 62 national and 21 international participants. The information gathered has been very interesting and in some aspects surprising. All the participants have already received the evaluation of each lab and the general summary. I just want to thank in a collective manner to all who participated. Ren? J. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From akemiat3377 <@t> yahoo.com Sat Apr 14 14:22:30 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Sat Apr 14 14:22:34 2007 Subject: [Histonet] (no subject) In-Reply-To: <20070414003603.18513.qmail@web36207.mail.mud.yahoo.com> Message-ID: <266096.65226.qm@web31302.mail.mud.yahoo.com> It makes it difficult to read your comments when every word is in CAP'S. Perhaps, you could just make the important word's in CAP's. Sometimes, MORE is not necessarily better. Akemi --- Jonathan De La Rosa wrote: > RAPID MODIFIED GIEMSA? A SHORT CUT DQ STAIN! WHY NOT > DO THE "MODIFIED GIEMSA" ON A ROUTINE BASES BEFORE > THE HIGHER COST IHC IS PERFORMED? > > > JD > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Maxim_71 <@t> mail.ru Sat Apr 14 13:38:38 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Apr 14 14:26:52 2007 Subject: [Histonet] Re: Demonstration of H. Pylori Message-ID: <1622433868.20070414223838@mail.ru> We use now Loeffler's stain (methylene blue) for H.pylori. It is very quickly and simply method. We use oil immersion for value. We have 3-16 GI bx daily (every gastric biopsy stain H&E, AB-PAS and Loeffler; we do not IHC). I also seen few times Helicobacter heilmannii in past year. And we never used H&E for values H.pylori. Maxim Peshkov Russia Taganrog From liz <@t> premierlab.com Sat Apr 14 15:11:59 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Apr 14 14:58:52 2007 Subject: [Histonet] ZN stain In-Reply-To: <4620BC54.A704.0070.0@uct.ac.za> Message-ID: <000001c77ed1$28bba800$0d00a8c0@domain.Premier> Michelle I think that what you are describing does happen. We handle quite a few samples from TB research and there is some discussion that sometimes the cell wall of the bacteria changes and is no longer acid fast. We use several methods here depending upon the project to look at TB in tissues sections the most common one is the Ziehl-Neelsen stain, we have also tried Wade-Fites modification for Leprosy, personally I did not see any differences between the two stains in guinea pig tissues exposed to m TB. We have also used a few IHC protocols, one being an anti-BCG antibody, but you need to be careful with this one in working up the protocol we also found that it also can stain gram positive bacteria, and it is not entirely specific to m. tuberculosis, which I believe is stated right on the spec sheet. There are also some home grown antibodies that we have worked on derived from culture supernates and some of those work also. Because we are working directly with the researchers on these projects we also have used some really nice RNA probes that are specific to m. tuberculosis. We have done extensive dual staining with anti-BCG, acid fast staining and in-situ probes and there are instances in which they are all staining different organisms. I'm going to forward you in a different e-mail a few papers and some contact information for a researcher up at Colorado State University who we work with on occasion to run the probes on human tissue samples. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Perrins Sent: Saturday, April 14, 2007 3:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ZN stain Hi Does anyone have a situation when staining for mycobacterium tuberculosis with the ZN stain, get negative results in the tissue sampled but the control is positive, in cases where the patient/deceased is known to have had TB. I work with autopsy material and have this situation when we know the person had TB but is negative in the test. Anyone have any other stain to demonstrate TB bacilli or information on this issue. Many thanks Michelle Forensic Pathology Services Western Cape South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From barry_m <@t> ozemail.com.au Sat Apr 14 19:49:31 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Sat Apr 14 19:49:39 2007 Subject: [Histonet] HSV 1&2 staining In-Reply-To: <041320071540.27129.461FA47A000E13DA000069F92200745672CDCEC9CF9D030706@comcast.net> References: <041320071540.27129.461FA47A000E13DA000069F92200745672CDCEC9CF9D030706@comcast.net> Message-ID: <000001c77ef7$f08776e0$d19664a0$@com.au> Hi Jim, We prepare a antibody cocktail of HSV I & II both from DAKO. HSV1 1:400 HSV2 1:800 No pre-treatment and 15 minute incubation in the antibody cocktail. No heat On a Vision BioSystem BONDMAX using the Polymer Define Detection System. I am certain it will work just as well on the DAKO Autostainer. Regards Barry Madigan HT (ADCLT) Immunohistochemistry QHPS-RBH Royal Brisbane Hospital Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jimr0712@comcast.net Sent: Saturday, 14 April 2007 1:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HSV 1&2 staining Could someone share the current methodology that they are using on the DAKO Autostainer Plus for HSV 1 & 2 staining ? Thanks. -- Jim Robinson, M.S., HTL/HT (ASCP) Director of Laboratory Operations Orizon Diagnostics, LLC 102 Chestnut Avenue Westmont, Illinois 60559 jrobinson@orizondiagnostics.com 630-321-1506 (fax) 630-230-6325 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdhisto <@t> yahoo.com Sun Apr 15 10:02:34 2007 From: jdhisto <@t> yahoo.com (Jonathan De La Rosa) Date: Sun Apr 15 10:02:44 2007 Subject: [Histonet] shouting ? Message-ID: <243980.58003.qm@web36201.mail.mud.yahoo.com> IM SORRY TO ACTUALLY HAVE TO POST A MESSAGE THAT DOES NOT PERTAIN TO HISTOLOGY OR THE ACTUALL REASON I POSTED A COMMENT IN THE FIRST PLACE REGARDING H/P STAINING AND IHC. BUT WHEN I WRIGHT IN CAPS ITS NOT TO BE RUDE AT ALL OR THAT I WOULD LIKE TO "SHOUT" MY OPINION'S. BUT IM SHURE LIKE MOST PEOPLE HERE I DONT HAVE MUCH TIME TO WORRY ABOUT LITTLE THINGS LIKE CAPS OR SPELLING OR MISS SPELLING. IM JUST INTERESTED IN VIEWING OTHER OPINIONS, GIVING AND RECIEVING VALUABLE INFORMATION MOSTLY PERTAINING TO HISTOLOGY, SER. PATH AND OTHER ANATOMIC PATH. ONCE AGAIN THANKS FOR YOUR COMMENTS AND SORRY ABOUT THE CAPS. GOTTA RUN.... JD From rjbuesa <@t> yahoo.com Sun Apr 15 10:18:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 15 10:18:31 2007 Subject: [Histonet] shouting ? In-Reply-To: <243980.58003.qm@web36201.mail.mud.yahoo.com> Message-ID: <334174.27373.qm@web61219.mail.yahoo.com> Jonathan: I seldom intervene in this type of exchange, but I think I will in this case. Your "disregarding" answer is somewhat arrogant and does not fit well with the general practice in Histonet. It takes the same time to type in lower case than in capitals and it is the norm. Society is ruled by norms and you are disregarding them. Do not expect having your concerns well received if your attitude is the one you expressed. Being in a hurry is no excuse. Ah, by the way: you " write" NOT "WRIGHT" (shouting now!) Ren? J. Jonathan De La Rosa wrote: IM SORRY TO ACTUALLY HAVE TO POST A MESSAGE THAT DOES NOT PERTAIN TO HISTOLOGY OR THE ACTUALL REASON I POSTED A COMMENT IN THE FIRST PLACE REGARDING H/P STAINING AND IHC. BUT WHEN I WRIGHT IN CAPS ITS NOT TO BE RUDE AT ALL OR THAT I WOULD LIKE TO "SHOUT" MY OPINION'S. BUT IM SHURE LIKE MOST PEOPLE HERE I DONT HAVE MUCH TIME TO WORRY ABOUT LITTLE THINGS LIKE CAPS OR SPELLING OR MISS SPELLING. IM JUST INTERESTED IN VIEWING OTHER OPINIONS, GIVING AND RECIEVING VALUABLE INFORMATION MOSTLY PERTAINING TO HISTOLOGY, SER. PATH AND OTHER ANATOMIC PATH. ONCE AGAIN THANKS FOR YOUR COMMENTS AND SORRY ABOUT THE CAPS. GOTTA RUN.... JD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From akemiat3377 <@t> yahoo.com Sun Apr 15 11:51:29 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Sun Apr 15 11:51:37 2007 Subject: [Histonet] Akemi's Response to "Shooting!" Be Kind Message-ID: <857666.1054.qm@web31307.mail.mud.yahoo.com> When posting my comment regarding CAP's to JD, I did not realize it would be taken in such a manner. It is truly difficult for me to read sentences when they are all capitalized. My eyesight isn't what it used to be!! I have been in the professional field of histology, since 1965 and am still learning something new every day. When I was younger, I was pretty arrogant as well. Maybe, it wasn't that long ago! Along the way, I met an became friends with such wonderful gracious people as Dezna Sheehan, Lee Luna, Chuck Churukian and Rich Horobin. They were and still are such inspirational individuals who took me under their wing. I can't remember a time when they were not available to me for trouble-shooting any issues regarding histology. They were always understanding of my motives for learning and trying to produce the best results as possible. I have to remind myself sometimes on their humbleness and hopefully it will rub off on me. Before leaving Biocare, I was involved with global technical support and trouble-shooting a special stains line I developed. I have learned to be a more patient person that has BIG EARS. It usually turns out to be 95% pilot error, but we have to be empathic and open to all possibilities. This is how to gain trust and respect. After all, we are all human and most of us hate to admit our mistakes. Warmest Regards, Akemi Allison-Tacha From greg.dobbin <@t> gmail.com Sun Apr 15 19:03:02 2007 From: greg.dobbin <@t> gmail.com (Greg Dobbin) Date: Sun Apr 15 19:03:08 2007 Subject: [Histonet] I'm back in Histonet circulation! Message-ID: Hello Colleagues, After a 10 month hiatus or so, I am back on the Histonet. It looked as though my career was heading away from histology but I am back in to it bigger than ever and I am quite excited about my new opportunity running a small histology department. I used to be in veterinary research so I will have plenty of questions for you all in the weeks and months to come and I very much look forward to re-establishing former Histonet aquaintences and friendships. Bye for now. Greg -- Greg Dobbin Chief Technologist Histology Queen Elizabeth Hospital Charlottetown, Prince Edward Island, Canada From greg.dobbin <@t> gmail.com Sun Apr 15 19:10:38 2007 From: greg.dobbin <@t> gmail.com (Greg Dobbin) Date: Sun Apr 15 19:10:42 2007 Subject: [Histonet] Oil Red O Controls Message-ID: Hello All, I am interested in hearing what other labs are doing with regard to having good positive controls slides on hand. Are frozen sections cut from normal breast tissue and stored? How? Fixed or unfixed? Room temp or freezer? Thanks in advance! -- Greg Dobbin Chief Technologist Histology Queen Elizabeth Hospital Charlottetown, PE Canada Ph (902) 894-2337 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 16 01:56:07 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 16 01:56:09 2007 Subject: [Histonet] ZN stain Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124753A@wahtntex2.waht.swest.nhs.uk> If memory serves TB bacilli maybe difficult to impossible to demonstrate in tissues from people that succumb to TB; but I can't recollect why. Is it to do with it being a focal disease and you need to be able to locate the 'hot spot'? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Progress in every age results only from the fact that there are some men and women who refuse to believe that what they know to be right cannot be done. --Russell W. Davenport This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 16 02:11:17 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 16 02:11:19 2007 Subject: [Histonet] Re: Fixative Formula Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124753B@wahtntex2.waht.swest.nhs.uk> Formol 37-40% HCHO 10 ml Dist Water 10 ml Absolute ethanol 80 ml "Tellyesniczky recommended a similar fluid to the last, containing 50 ml each of formalin and acetic acid and 1000 ml alcohol. This fluid is to be distinguished from the author's acetic dichromate formula." R.D. Lillie 'Histologic Technic and Practical Histochemistry' 4th Edition. McGraw-Hill 1976, ISBN 0-07-037862-2 Tellyesniczky's fluid Tellyesnic Fekete Opie Lillie's Bodian Zky's & Lavin AAF 37-40% formaldehyde 5ml 10ml 5ml 10ml 5ml Glacial acetic acid 5ml 5ml 5ml 5ml 5ml Alcohol Concentration 70% 70% 80% 95-100% 50% Amount 100ml 100ml 90ml 85ml 100ml Page 34 of Lillie's esteemed volume. "Trying to keep the old fixatives alive" Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Progress in every age results only from the fact that there are some men and women who refuse to believe that what they know to be right cannot be done. --Russell W. Davenport This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 16 03:35:30 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 16 03:35:35 2007 Subject: [Histonet] Demonstration of H. Pylori Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247543@wahtntex2.waht.swest.nhs.uk> "One of the girls did a project comparing 6 or 7 stains and the best was ipox,as one would expect. The next best was Giema, in that it gave the best staining against background light staining, most consistently. Silver stains were pretty hopeless. Tol. Blue easily overstained, and others were too laborious." Terry 'girls', 'girls'!! Oh you mean highly trained MSc qualified female Healthcare Scientists, you had me for a while there. What did the boys think of the project? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Progress in every age results only from the fact that there are some men and women who refuse to believe that what they know to be right cannot be done. --Russell W. Davenport This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jqb7 <@t> cdc.gov Mon Apr 16 05:19:44 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Apr 16 05:19:51 2007 Subject: [Histonet] FW: HTL question References: Message-ID: That's what we were afraid of. Thanks. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _____ From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Saturday, April 14, 2007 1:04 AM To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Bartlett, Jeanine (CDC/CCID/NCZVED) Subject: RE: [Histonet] FW: HTL question There is more than one answer wrong in the study guide. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2007 08:47 AM To "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , cc Subject RE: [Histonet] FW: HTL question C is the best answer. D is plain wrong. Moreover, it is ridiculous. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 11 April 2007 18:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: HTL question All, I am forwarding this email from a young lady I know that is studying for her HTL. I will pass along your responses. Thanks in advance for helping. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov > ______________________________________________ > From: Packard, Michelle (CDC/CCID/NCZVED) > Sent: Wednesday, April 11, 2007 1:21 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED) > Subject: RE: HTL question > > The following question is in the Histotechnology BOR Study Guide > Practice Questions 2nd Edition published by ASCP: > > For light microscopic evaluation it is generally necessary to use > special stains to demonstrate fungi in tissue sections because fungi: > A. can only be seen using silver impregnation > B. are removed in the routine staining process > C. stain variably with the H&E procedure > D. are never demonstrated with routine procedures > > The consensus here is that C is the best answer, however, the answer > key lists D as correct. Would like to get some feedback on this if > possible. Is the answer key wrong or am I missing something? > > In preparing for the exam, has anyone run across any other answers > from this text that appear to be wrong? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Apr 16 05:41:03 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Apr 16 05:41:05 2007 Subject: [Histonet] Proper Decalcification In-Reply-To: <20070413154039.D1DF032B0@centaur.cnchost.com> Message-ID: We use the Faxitron. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Friday, April 13, 2007 10:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Proper Decalcification I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 16 07:48:57 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 16 07:49:01 2007 Subject: [Histonet] Oil Red O Controls In-Reply-To: Message-ID: <804452.17014.qm@web61223.mail.yahoo.com> I used to cut FS of (+) materials and stored UNFIXED in a -80?C freezer. They were useful for several years. Ren? J. Greg Dobbin wrote: Hello All, I am interested in hearing what other labs are doing with regard to having good positive controls slides on hand. Are frozen sections cut from normal breast tissue and stored? How? Fixed or unfixed? Room temp or freezer? Thanks in advance! -- Greg Dobbin Chief Technologist Histology Queen Elizabeth Hospital Charlottetown, PE Canada Ph (902) 894-2337 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From TMcNemar <@t> lmhealth.org Mon Apr 16 07:52:30 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Apr 16 07:52:41 2007 Subject: [Histonet] H. Pylori IHC In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F47F@lmhsmail.lmhealth.org> We automatically do IHC for H Pylori on all gastric biopsies. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dolores Townsend Sent: Friday, April 13, 2007 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori IHC One more question for those who do IHC: do you do these routinely on all gastric biopsies, or only as requested by your pathologist? Thanks, Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wfubmc.edu Mon Apr 16 07:56:55 2007 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Mon Apr 16 07:57:02 2007 Subject: [Histonet] Hard Tissue members Message-ID: <9AEEF1FB6254224AA355ED285F849165212812EF@EXCHVS2.medctr.ad.wfubmc.edu> The following Hard Tissue members had their copies of the most recent HTC newsletter returned to me due to "undeliverable at this address". If you are still members of NSH and would like to continue to receive your copies of the newsletter in the future, please e-mail me your current address. Thanks! Hermina Jennifer Stashevsky Bruce Shroyer Simon Smith Loralee Gehan Connie McManus Crystal Idleburg Hermina M. Borgerink, BA, HT, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax. (336) 716-1515 e-mail hborgeri@wfubmc.edu "Treat a man as he appears to be, and you make him worse. But treat a man as if he already were what he potentially could be, and you make him what he should be." (Goethe) This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. Thank you. From Mary.Vaughan <@t> RoswellPark.org Mon Apr 16 07:58:22 2007 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Mon Apr 16 07:58:26 2007 Subject: [Histonet] PDGF alpha, beta and receptors Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE1A6E8E6B@VERITY.roswellpark.org> good morning everyone- I have a proposed project that involves staining of PDGF alpha, beta, and the receptor for each. Does anyone have these protocols worked out? I'd appreciate some help, thanks. [PDGF-A, PDGFR-a, PDGF-B, PDGFR-b] Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From BMolinari <@t> heart.thi.tmc.edu Mon Apr 16 08:31:48 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Apr 16 08:31:51 2007 Subject: [Histonet] Proper Decalcification In-Reply-To: Message-ID: Faxitron MX 20 Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Monday, April 16, 2007 5:47 AM To: Molinari, Betsy Subject: RE: [Histonet] Proper Decalcification Which model do you use? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, April 16, 2007 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification We use the Faxitron. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Friday, April 13, 2007 10:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Proper Decalcification I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Mon Apr 16 08:35:20 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Apr 16 08:35:28 2007 Subject: [Histonet] derm path Message-ID: <8C94E43364E2C99-F80-89E6@WEBMAIL-MB18.sysops.aol.com> Hello all, We are thinking of adding another client, but it is a derm client. Can anybody tell me what all antidoies I will need to have on hand? I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? Thanks in advance, Roxanne ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rjbuesa <@t> yahoo.com Mon Apr 16 09:16:50 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 16 09:16:57 2007 Subject: [Histonet] derm path In-Reply-To: <8C94E43364E2C99-F80-89E6@WEBMAIL-MB18.sysops.aol.com> Message-ID: <603210.72760.qm@web61218.mail.yahoo.com> Think also on Vimentin, Cam 5.2, AE1/AE3 CK5/6, Collagen IV, EMAg, EGFR, Factor VIII and don't forget that some pathologists will request UEA-1 lectin with the Anti-Ulex Europaeus Ren? J. godsgalnow@aol.com wrote: Hello all, We are thinking of adding another client, but it is a derm client. Can anybody tell me what all antidoies I will need to have on hand? I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? Thanks in advance, Roxanne ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From Rcartun <@t> harthosp.org Mon Apr 16 09:30:35 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 16 09:30:53 2007 Subject: [Histonet] derm path In-Reply-To: <8C94E43364E2C99-F80-89E6@WEBMAIL-MB18.sysops.aol.com> References: <8C94E43364E2C99-F80-89E6@WEBMAIL-MB18.sysops.aol.com> Message-ID: <4623504B0200007700005539@gwmail.harthosp.org> I think the more important issues here are 1.) how much experience do you have doing immunohistochemical (IHC) staining and 2.) what will be your volume as you move forward. Keep in mind that many DermPath diagnoses are based on IHC findings and you need to perform validations on-site (for every primary antibody) to demonstrate that you are obtaining the correct IHC results. Are you prepared to deal with issues concerning fixation and tissue processing, antigen retrieval, nonspecific staining, antibody cross-reactivity, appropriate negative and positive controls, etc.? In my opinion, you should not be doing IHC unless you are performing the tests on a regular basis and you have an experienced staff to do the job right, including a pathologist to assist and support you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 04/16/07 9:35 AM >>> Hello all, We are thinking of adding another client, but it is a derm client. Can anybody tell me what all antidoies I will need to have on hand? I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? Thanks in advance, Roxanne ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From gcallis <@t> montana.edu Mon Apr 16 09:44:43 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Apr 16 09:44:38 2007 Subject: [Histonet] component substitute in Spurrs In-Reply-To: References: <20070413151750.D570844828F@phsmgmx3.partners.org> <6.0.0.22.1.20070413124646.01b8f8f8@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070416084236.01b26290@gemini.msu.montana.edu> Dear Phil, Thanks for the update for the new component in Spurrs. Please PDF the information to me privately. At 01:18 PM 4/13/2007, you wrote: >Spurr's as such has not been discontinued, but one of its components has. >Which is good, it's my favorite resin for both plants and animals. >ERL 4206, VCD, is no longer available, and ERL 4221 has been substituted. >4221 is Cycloaliphatic Epoxide Resin. >The suppliers were (may still be) claiming that 4221 is a straight 1:1 >substitute for 4206, but it isn't. Making Spurr's with the new component >is covered in: >E. Ann Ellis >Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene >Dioxide in Spurr Low Viscosity Embedding Formulations >Microscopy Today, July 2006 14(4):32 ff > >I can a PDF of the article to anyone who wants it. > >Phil > >>I had heard that Spurrs was discontinued. Hopefully there is a >>substitute and contacting Energy Beam Sciences may be one way to find >>out. Can anyone enlighten us on this? >> >>Gayle Callis >>MT,HT,HTL(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University - Bozeman >>PO Box 173610 >>Bozeman MT 59717-3610 From Terry.Marshall <@t> rothgen.nhs.uk Mon Apr 16 10:10:21 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Apr 16 10:10:31 2007 Subject: [Histonet] Demonstration of H. Pylori Message-ID: <407F05A128805F4C879A33DBA32E618E20C2C5@TRFT-EX01.xRothGen.nhs.uk> Not necessarily. In fact she was some junior version of MLSO or scientist or whatever you call yourselves this week. The fact remains she was a girl. I still recognise those things even if you had a problem remembering. My gout prevents my memory being of use, 'cos I can't give chase any more. The boys didn't think at all, because we don't have them, except the big boss administrator and the AP cytology. Terry PS Talking of Spurr, whatever happened to Taab? ________________________________ From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 16 April 2007 09:36 To: Marshall Terry Dr, Consultant Histopathologist; Dolores Townsend; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Demonstration of H. Pylori "One of the girls did a project comparing 6 or 7 stains and the best was ipox,as one would expect. The next best was Giema, in that it gave the best staining against background light staining, most consistently. Silver stains were pretty hopeless. Tol. Blue easily overstained, and others were too laborious." Terry 'girls', 'girls'!! Oh you mean highly trained MSc qualified female Healthcare Scientists, you had me for a while there. What did the boys think of the project? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Progress in every age results only from the fact that there are some men and women who refuse to believe that what they know to be right cannot be done. --Russell W. Davenport This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From godsgalnow <@t> aol.com Mon Apr 16 10:36:57 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Apr 16 10:37:43 2007 Subject: [Histonet] derm path In-Reply-To: <4623504B0200007700005539@gwmail.harthosp.org> References: <8C94E43364E2C99-F80-89E6@WEBMAIL-MB18.sysops.aol.com> <4623504B0200007700005539@gwmail.harthosp.org> Message-ID: <8C94E5433EA14A2-F80-9124@WEBMAIL-MB18.sysops.aol.com> Thank you for your input. I have an extensive knowledge of IHC and am familiar with all of the concerns that you mentioned in your email. I do IHC and FISH everyday, I was just questioning what other antibodies could they ask for, in addition to the more common ones. I asked this question not because of inexperience, but because I have been out of the hospital setting for a few years and currently the specimens I receive are GI and GU. Roxanne Soto HT(ASCP)QIHC Lab Supervisor Physicians RightPath Tampa, Fl -----Original Message----- From: Rcartun@harthosp.org To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Sent: Mon, 16 Apr 2007 10:30 AM Subject: Re: [Histonet] derm path I think the more important issues here are 1.) how much experience do you have doing immunohistochemical (IHC) staining and 2.) what will be your volume as you move forward. Keep in mind that many DermPath diagnoses are based on IHC findings and you need to perform validations on-site (for every primary antibody) to demonstrate that you are obtaining the correct IHC results. Are you prepared to deal with issues concerning fixation and tissue processing, antigen retrieval, nonspecific staining, antibody cross-reactivity, appropriate negative and positive controls, etc.? In my opinion, you should not be doing IHC unless you are performing the tests on a regular basis and you have an experienced staff to do the job right, including a pathologist to assist and support you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 04/16/07 9:35 AM >>> Hello all, We are thinking of adding another client, but it is a derm client. Can anybody tell me what all antidoies I will need to have on hand? I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? Thanks in advance, Roxanne ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From JGarfield <@t> lifecell.com Mon Apr 16 11:43:50 2007 From: JGarfield <@t> lifecell.com (Jacqueline D. Garfield) Date: Mon Apr 16 11:43:57 2007 Subject: [Histonet] component substitute in Spurrs Message-ID: Hello Phil, May I please have a PDF of the article, also? Thank you, Jackie Garfield LifeCell Corporation jgarfield@lifecell.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, April 16, 2007 10:45 AM To: Philip Oshel; Histonet@lists.utsouthwestern.edu Subject: [Histonet] component substitute in Spurrs Dear Phil, Thanks for the update for the new component in Spurrs. Please PDF the information to me privately. At 01:18 PM 4/13/2007, you wrote: >Spurr's as such has not been discontinued, but one of its components has. >Which is good, it's my favorite resin for both plants and animals. >ERL 4206, VCD, is no longer available, and ERL 4221 has been substituted. >4221 is Cycloaliphatic Epoxide Resin. >The suppliers were (may still be) claiming that 4221 is a straight 1:1 >substitute for 4206, but it isn't. Making Spurr's with the new component >is covered in: >E. Ann Ellis >Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene >Dioxide in Spurr Low Viscosity Embedding Formulations >Microscopy Today, July 2006 14(4):32 ff > >I can a PDF of the article to anyone who wants it. > >Phil > >>I had heard that Spurrs was discontinued. Hopefully there is a >>substitute and contacting Energy Beam Sciences may be one way to find >>out. Can anyone enlighten us on this? >> >>Gayle Callis >>MT,HT,HTL(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University - Bozeman >>PO Box 173610 >>Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From billingconsultants <@t> yahoo.com Mon Apr 16 12:34:36 2007 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Mon Apr 16 12:34:40 2007 Subject: [Histonet] Derm Path Message-ID: <51193.16546.qm@web54215.mail.yahoo.com> Hi, They may also ask for CD34 and Factor XIIIA (13A). In my experience, the list you've been given and what you started with will cover most of them. Kindest regards, Louri Roberts Consultant www.billingconsultants.net --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From TJJ <@t> Stowers-Institute.org Mon Apr 16 13:05:30 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Apr 16 13:05:53 2007 Subject: [Histonet] RE: Derm path In-Reply-To: Message-ID: Roxanne - depends too on whether you will be doing direct immunofluorescence for them as well. I imagine a busy derm practice would require the capability of doing them. Therefore, FITC-conjugated IgG, IgA, IgM, C3 and Fibrinogen might be useful. (Disclaimer: this panel may have changed since I left clinical.) Also, you may have to have the leukemia/lymphoma markers on hand because cutaneous lymphomas are usually biopsied by Dermatologists. Talk with your pathologist as to the panel he/she might recommend for typing these. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Valerie.Hannen <@t> parrishmed.com Mon Apr 16 13:11:44 2007 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Apr 16 13:11:51 2007 Subject: [Histonet] Zinc Formalin and Iron stains Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34A8@ISMAIL.parrishmed.local> Hi fellow histonetters!! We are just wondering if anyone else has run into this problem...we have been told by our Pathologist that smears that we make from a Bone Marrow aspirate (before fixation) show positive Iron staining... however, the clot from the same aspirate that has been fixed in Zinc Formalin showed no Iron staining. Has Zinc Formalin been known to leach Iron out of Bone Marrow aspirates? Any insight that you could share with us would be greatly appreciated. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From mtarango <@t> nvcancer.org Mon Apr 16 13:32:53 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Apr 16 13:33:09 2007 Subject: [Histonet] Zinc Formalin and Iron stains In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB34A8@ISMAIL.parrishmed.local> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6C7C@NVCIEXCH02.NVCI.org> I don't know, we only use 10% NBF for bone marrows here, but I've seen a video of some cells getting fixed with zinc formalin. The cells were popping and exploding all over the place. Made me think, "no wonder you get that great nuclear detail." Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, April 16, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Zinc Formalin and Iron stains Hi fellow histonetters!! We are just wondering if anyone else has run into this problem...we have been told by our Pathologist that smears that we make from a Bone Marrow aspirate (before fixation) show positive Iron staining... however, the clot from the same aspirate that has been fixed in Zinc Formalin showed no Iron staining. Has Zinc Formalin been known to leach Iron out of Bone Marrow aspirates? Any insight that you could share with us would be greatly appreciated. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************ ************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Rcartun <@t> harthosp.org Mon Apr 16 13:39:11 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 16 13:39:22 2007 Subject: [Histonet] Visionbiosystems Bond In-Reply-To: <000801c77d09$4ffe44f0$0711c847@D7XQNX91> References: <000801c77d09$4ffe44f0$0711c847@D7XQNX91> Message-ID: <46238A8F0200007700005562@gwmail.harthosp.org> I've had two Bond Max instruments in my laboratory since last December (I also have 4 Dako autostainers). The machine is incredible; very simple to use (even I can do it). It does everything except coverslip the slide. Each machine will stain up to 30 slides and you have the option of continuous throughput. You can start 10 slides, then add another 10, and so on. I have never seen more "robust" immunohistochemical stains. However, you will need to re-titer your primary antibodies, but this is a good thing. Using their Bond Polymer Refine detection kit, we have found that we can dilute our primaries 1 or 2 fold and get better reactivity than what we saw before. They offer a beautiful alkaline phosphatase (red) detection kit as well. You pre-select the antigen retrieval when you input your antibodies into the machine's computer. The low and high pH antigen retrievals on the machine are very good, and there is never any tissue loss (even with their high pH). I like the fact that I can change the antigen retrieval depending on the type of specimen. For example, if I am staining a core biopsy from a lymph node that was "rushed" processed for CD5, I can change the high pH retrieval from 20 minutes to 10 minutes very easily, whereas I can keep it at 20 minutes for our over-fixed tonsil positive control. My only concern is the cost per slide, but I was told by Vision BioSystems that they are competitive. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marian Powers" 04/12/07 9:48 AM >>> Hello all: Anyone out there using the Bond? What are the advantages and disadvantages? Any hidden costs, etc.? Thanks in advance. Marian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From gcallis <@t> montana.edu Mon Apr 16 13:59:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Apr 16 13:59:13 2007 Subject: [Histonet] Zinc Formalin and Iron stains In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDE011B6C7C@NVCIEXCH02.NVCI.o rg> References: <5680DA93771F0C48954CC8D38425E72401AB34A8@ISMAIL.parrishmed.local> <5AEC610C1CE02945BD63A395BA763EDE011B6C7C@NVCIEXCH02.NVCI.org> Message-ID: <6.0.0.22.1.20070416125416.01b3d438@gemini.msu.montana.edu> We have used zinc formalin, although it was Anatech's buffered zinc formalin formulation, and had wonderful results on human kidney biopsies - excellent morphology and no exploded cells that the pathologist could see. He loved the results. The sections cut at 1 - 2 um. When we tried this fixative on animal tissues, we also had great success. Could it be it was a zinc formalin that was NOT buffered correctly or even at all that caused the exploding cells? As for iron staining after zinc formalin, not sure what the results are. However, you should speak to Anatech, in particular, Ada Feldman, she could let you know if iron staining is compromised by this fixative. At 12:32 PM 4/16/2007, you wrote: >I don't know, we only use 10% NBF for bone marrows here, but I've seen a >video of some cells getting fixed with zinc formalin. The cells were >popping and exploding all over the place. Made me think, "no wonder you >get that great nuclear detail." Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From wia2005 <@t> med.cornell.edu Mon Apr 16 14:14:09 2007 From: wia2005 <@t> med.cornell.edu (William Ares) Date: Mon Apr 16 14:14:15 2007 Subject: [Histonet] propidium iodide protocol Message-ID: <5f4fef8e23ad.462392c1@med.cornell.edu> Does anyone have a protocol that they can share for use of propidium iodide to assess cell death in culture (medulloblastoma culture, if that makes a difference)? I'm using both 96 well plates and 35ml flasks and Sigma Aldrich's Propidium Iodide Solution. Thanks. William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu From ROrr <@t> enh.org Mon Apr 16 14:25:31 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Apr 16 14:25:37 2007 Subject: [Histonet] Derm Path Message-ID: Hi Roxanne, I see you've got a few responses to this querry. Our Derm Path who also happens to be the big cheese around here runs differential markers for Lymphomas in the Dermis. We run quite a few CD markers(CD 3,4,5, 7, 8,20 to name a few) as well as those mentioned by the others. Our Derm Path also requests PAS(fungus), Brown/Hopps, and Colloidal Iron on the majority. Plus AFB and GMS on a few. I would never DARE call this overkill, as I have no idea what needs to be diagnosed on myriad of skins that come through our department. I just call it Job security. (as if....) My question would be, do you have a pathologist interested in this specialty, or would you just run the stains and then send them to a Dermpath specialist? Becky Becky Orr, CLA, HT(ASCP) QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 Message: 13 Date: Mon, 16 Apr 2007 09:35:20 -0400 From: godsgalnow@aol.com Subject: [Histonet] derm path To: histonet@lists.utsouthwestern.edu Message-ID: <8C94E43364E2C99-F80-89E6@WEBMAIL-MB18.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello all, We are thinking of adding another client, but it is a derm client. Can anybody tell me what all antidoies I will need to have on hand? I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? Thanks in advance, Roxanne ********************** From llewllew <@t> shaw.ca Mon Apr 16 14:36:23 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Apr 16 14:37:58 2007 Subject: [Histonet] Picric acid Message-ID: <000501c7805e$84949680$6500a8c0@yourlk4rlmsu> I thought Histonetters might be interested in this news article. http://www.cbc.ca/cp/Oddities/070416/K041602AU.html Bryan Llewellyn From liz <@t> premierlab.com Mon Apr 16 14:55:06 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Apr 16 14:42:04 2007 Subject: [Histonet] Zinc Formalin and Iron stains In-Reply-To: <6.0.0.22.1.20070416125416.01b3d438@gemini.msu.montana.edu> Message-ID: <000001c78061$21916bf0$0d00a8c0@domain.Premier> Gayle is correct if the zinc formalin is not buffered. When I initially worked with this fixative in the mid eighty's it was not commercially available. If you made this solution up from scratch its pH was between 1 and 2, so very acidic. I believe you can only purchase the buffered form through a vendor and you can't make it up from scratch, the zinc will come out of solution when you try to make it up in a buffer. Iron can be removed in acid solutions, that's why it can be sometimes difficult to see iron in decalcified bone marrow cores. Just search the archives for iron and bone marrow and you'll see lots of responses. Peggy Wenk has a nice post that goes over what you need to do to retain the iron in bone marrow specimens, she also states in that post that iron can be removed in fixatives that are acidic and to make sure the fixative that you use is buffered and is at the correct pH. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, April 16, 2007 12:59 PM To: Tarango, Mark; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Zinc Formalin and Iron stains We have used zinc formalin, although it was Anatech's buffered zinc formalin formulation, and had wonderful results on human kidney biopsies - excellent morphology and no exploded cells that the pathologist could see. He loved the results. The sections cut at 1 - 2 um. When we tried this fixative on animal tissues, we also had great success. Could it be it was a zinc formalin that was NOT buffered correctly or even at all that caused the exploding cells? As for iron staining after zinc formalin, not sure what the results are. However, you should speak to Anatech, in particular, Ada Feldman, she could let you know if iron staining is compromised by this fixative. At 12:32 PM 4/16/2007, you wrote: >I don't know, we only use 10% NBF for bone marrows here, but I've seen a >video of some cells getting fixed with zinc formalin. The cells were >popping and exploding all over the place. Made me think, "no wonder you >get that great nuclear detail." Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From AGrobe2555 <@t> aol.com Mon Apr 16 15:32:05 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Apr 16 15:32:19 2007 Subject: [Histonet] Picric acid Message-ID: It probably would have been easier to add some water to the bottle......and less expensive too. ************************************** See what's free at http://www.aol.com. From micro <@t> superlink.net Mon Apr 16 15:48:52 2007 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Mon Apr 16 15:49:22 2007 Subject: [Histonet] Picric acid References: <000501c7805e$84949680$6500a8c0@yourlk4rlmsu> Message-ID: <00bb01c78068$a56df770$13893cd1@DJ4VDH31> Don't panic now!!! That is why one stores Picric acid as a water saturated mixture! Never heard of an explosion of picric acid in a histo lab! Markus ----- Original Message ----- From: "Bryan Llewellyn" To: "Histonet" Sent: Monday, April 16, 2007 3:36 PM Subject: [Histonet] Picric acid >I thought Histonetters might be interested in this news article. > > http://www.cbc.ca/cp/Oddities/070416/K041602AU.html > > Bryan Llewellyn > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bhewlett <@t> cogeco.ca Mon Apr 16 16:08:31 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Apr 16 16:08:36 2007 Subject: [Histonet] Picric acid References: Message-ID: <000b01c7806b$63035fd0$6500a8c0@mainbox> How would you get the bottle open to add the water??? Moving the bottle or opening it could set it off. Dumb move!!! Bryan ----- Original Message ----- From: To: Sent: Monday, April 16, 2007 4:32 PM Subject: [Histonet] Picric acid > It probably would have been easier to add some water to the > bottle......and > less expensive too. > > > > > ************************************** See what's free at > http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From esther.peters <@t> verizon.net Mon Apr 16 16:15:20 2007 From: esther.peters <@t> verizon.net (Esther Peters) Date: Mon Apr 16 16:14:14 2007 Subject: [Histonet] Picric acid References: <000501c7805e$84949680$6500a8c0@yourlk4rlmsu> <00bb01c78068$a56df770$13893cd1@DJ4VDH31> Message-ID: <4623E768.4020003@verizon.net> A friend of mine working at a U.S. government lab was doing just that (had filled the bottle to the top with water, sealed around the lid with parafilm and tape to prevent dehydration) and the new safety officer who saw it called the bomb squad to remove it! Esther Peters George Mason University Markus F. Meyenhofer wrote: > Don't panic now!!! > That is why one stores Picric acid as a water saturated mixture! Never > heard of an explosion of picric acid in a histo lab! > Markus > ----- Original Message ----- From: "Bryan Llewellyn" > To: "Histonet" > Sent: Monday, April 16, 2007 3:36 PM > Subject: [Histonet] Picric acid > > >> I thought Histonetters might be interested in this news article. >> >> http://www.cbc.ca/cp/Oddities/070416/K041602AU.html >> >> Bryan Llewellyn >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jfish <@t> gladstone.ucsf.edu Mon Apr 16 16:37:08 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Mon Apr 16 16:37:41 2007 Subject: [Histonet] Picric acid In-Reply-To: <4623E768.4020003@verizon.net> Message-ID: <000301c7806f$62c03f80$8903010a@JFISH> Eeek! I had just run to check my Picric Acid. I added a little water to be on the safe side, although it was covered by more than 2cm of water. Then I opened this email. I've had this bottle for nearly 6 years (who knows how long it was here before I inherited it). We even moved it a couple miles over to our new building 2.5 years ago! The especially hired chemical movers never said a word! Glad it didn't explode and make CNN's prime time news! Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Esther Peters Sent: Monday, April 16, 2007 2:15 PM To: Markus F. Meyenhofer Cc: Histonet Subject: Re: [Histonet] Picric acid A friend of mine working at a U.S. government lab was doing just that (had filled the bottle to the top with water, sealed around the lid with parafilm and tape to prevent dehydration) and the new safety officer who saw it called the bomb squad to remove it! Esther Peters George Mason University Markus F. Meyenhofer wrote: > Don't panic now!!! > That is why one stores Picric acid as a water saturated mixture! Never > heard of an explosion of picric acid in a histo lab! > Markus > ----- Original Message ----- From: "Bryan Llewellyn" > > To: "Histonet" > Sent: Monday, April 16, 2007 3:36 PM > Subject: [Histonet] Picric acid > > >> I thought Histonetters might be interested in this news article. >> >> http://www.cbc.ca/cp/Oddities/070416/K041602AU.html >> >> Bryan Llewellyn >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Mon Apr 16 17:18:09 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Apr 16 17:18:31 2007 Subject: [Histonet] Picric acid In-Reply-To: <4623E768.4020003@verizon.net> References: <000501c7805e$84949680$6500a8c0@yourlk4rlmsu> <00bb01c78068$a56df770$13893cd1@DJ4VDH31> Message-ID: <4.3.2.7.2.20070416151101.02920d40@algranth.inbox.email.arizona.edu> Same thing happened to me a long time ago only it was a new lab manager who saw the bottle of picric acid stored pretty much the same way as you describe and pitched a fit. Not only did he call the bomb squad but I got written up for having it in the lab. Totally unfair! It was in the lab before I started working there, stored improperly in the Chemistry dept. Andi At 05:15 PM 4/16/2007 -0400, Esther Peters wrote: >A friend of mine working at a U.S. government lab was doing just that (had >filled the bottle to the top with water, sealed around the lid with >parafilm and tape to prevent dehydration) and the new safety officer who >saw it called the bomb squad to remove it! > >Esther Peters >George Mason University > >Markus F. Meyenhofer wrote: > >>Don't panic now!!! >>That is why one stores Picric acid as a water saturated mixture! Never >>heard of an explosion of picric acid in a histo lab! >>Markus >>----- Original Message ----- From: "Bryan Llewellyn" >>To: "Histonet" >>Sent: Monday, April 16, 2007 3:36 PM >>Subject: [Histonet] Picric acid >> >>>I thought Histonetters might be interested in this news article. >>> >>>http://www.cbc.ca/cp/Oddities/070416/K041602AU.html >>> >>>Bryan Llewellyn >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From jnocito <@t> satx.rr.com Mon Apr 16 17:28:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Apr 16 17:28:36 2007 Subject: [Histonet] Picric acid References: <000501c7805e$84949680$6500a8c0@yourlk4rlmsu> Message-ID: <01cc01c78076$985f09d0$11614542@yourxhtr8hvc4p> yeah, but look at the blast we could have had. Sorry, you know me, couldn't resist Jo Da To ----- Original Message ----- From: "Bryan Llewellyn" To: "Histonet" Sent: Monday, April 16, 2007 2:36 PM Subject: [Histonet] Picric acid >I thought Histonetters might be interested in this news article. > > http://www.cbc.ca/cp/Oddities/070416/K041602AU.html > > Bryan Llewellyn > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Apr 16 17:31:47 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Apr 16 17:31:39 2007 Subject: [Histonet] I'm back in Histonet circulation! References: Message-ID: <01f401c78077$0553a5a0$11614542@yourxhtr8hvc4p> welcome back Greg ----- Original Message ----- From: "Greg Dobbin" To: Sent: Sunday, April 15, 2007 7:03 PM Subject: [Histonet] I'm back in Histonet circulation! > Hello Colleagues, > After a 10 month hiatus or so, I am back on the Histonet. It looked as > though my career was heading away from histology but I am back in to it > bigger than ever and I am quite excited about my new opportunity running a > small histology department. > > I used to be in veterinary research so I will have plenty of questions for > you all in the weeks and months to come and I very much look forward to > re-establishing former Histonet aquaintences and friendships. > Bye for now. > Greg > > -- > > Greg Dobbin > > Chief Technologist > Histology > Queen Elizabeth Hospital > Charlottetown, Prince Edward Island, > Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From opiecurt <@t> yahoo.com Mon Apr 16 19:17:38 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Mon Apr 16 19:17:43 2007 Subject: [Histonet] need to fill 2 positions Message-ID: <780993.98225.qm@web81615.mail.mud.yahoo.com> finally got the go ahead and will need to add 2 techs. prefer a couple years experience. going to be doing a lot of embedding and cutting... staining and cover slipping is automated. a small amount of specials. basic computer skills and some occasional driving will be required when short staffed. also do a good bit of derm specimens.... all the basics. we're in southern California, San Bernardina and will be moving within a couple months to the Anaheim (Disneyland) area. there will be 2 shifts, early a.m. to mid day and late a.m. to early evening. probably rotate shifts for flexibility. respond to this email for more contact information and/or information. no website. curt pathology arts --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From Histosprv06 <@t> aol.com Mon Apr 16 21:50:04 2007 From: Histosprv06 <@t> aol.com (Histosprv06@aol.com) Date: Mon Apr 16 21:50:11 2007 Subject: [Histonet] (no subject) Message-ID: Hi Greg, I did about the same as Rene` J. I used fresh fatty breast tissue and froze it unfixed in OCT to cut for control +plus slides. I think if you fix it, it will not demonstrate the fat...the alcohol pulls it right out (on cytospin bronch washings anyway) . However, I stored it in a -60 degree freezer for future use. My controls always came out beautiful. They keep just fine! A nice, thick control block ought to last you ages. Good luck. Kari Zajic HT,MLT I used to cut FS of (+) materials and stored UNFIXED in a -80?C freezer. They were useful for several years. Ren? J. Greg Dobbin wrote: Hello All, I am interested in hearing what other labs are doing with regard to having good positive controls slides on hand. Are frozen sections cut from normal breast tissue and stored? How? Fixed or unfixed? Room temp or freezer? Thanks in advance! -- Greg Dobbin Chief Technologist Histology Queen Elizabeth Hospital Charlottetown, PE Canada Ph (902) 894-2337 ************************************** See what's free at http://www.aol.com. From e.kaplan <@t> uaeu.ac.ae Mon Apr 16 22:42:57 2007 From: e.kaplan <@t> uaeu.ac.ae (Evelyn Kaplan) Date: Mon Apr 16 22:39:55 2007 Subject: [Histonet] Oil Red O Controls In-Reply-To: <804452.17014.qm@web61223.mail.yahoo.com> Message-ID: <000a01c780a2$7d5a5dc0$afa2fea9@aa.uaeu.ac.ae> I have used a piece of fixed fish liver for many years (not the same one, that is! :-)) and it makes an easy source of fat control tissue. Evelyn Kaplan, Dept. of Medical Education, Chair, BSc MLT Programme, Faculty of Medicine & Health Sciences, Al Ain, UAE. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 16 April 2007 16:49 To: Greg Dobbin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O Controls I used to cut FS of (+) materials and stored UNFIXED in a -80?C freezer. They were useful for several years. Ren? J. Greg Dobbin wrote: Hello All, I am interested in hearing what other labs are doing with regard to having good positive controls slides on hand. Are frozen sections cut from normal breast tissue and stored? How? Fixed or unfixed? Room temp or freezer? Thanks in advance! -- Greg Dobbin Chief Technologist Histology Queen Elizabeth Hospital Charlottetown, PE Canada Ph (902) 894-2337 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rbwadley <@t> dodo.com.au Mon Apr 16 22:48:28 2007 From: rbwadley <@t> dodo.com.au (Rbwadley) Date: Mon Apr 16 22:48:35 2007 Subject: [Histonet] Picric acid Message-ID: Hi All, I was taught in my histopathology classes that picric acid was dangerous, in chemistry classes we were told of all the gags that can be played using it. Apparently it was used in Naval ammunitions because it explodes when slightly damp - when gun powder won't. Crytals spread on the flood in the days of hard, leather soled shoes created a crackling sound as you walked across the room. Crystals sprinkled on the bench and slapped with a ruler go 'pop' just like a fire cracker. More seriously it is also a neuro toxin. Many years ago the safety officer in the institute I was working in found a dried out jar of picric acid. While he was gingerly walking across the yard from the main building to the less used pump house (he was all dressed up in face shield, leather gloves and leather apron), the jar was safely contained in a 20L bucket of water, I was asking what he intended to do. It turned out that I sneaked into the pump house a couple of days later, gently loosened the lid (under water), retrieved the jar filled it with distilled water and had a nice stock of picric acid for the histo lab! A little bit of thought and care is much cheaper than the bomb squad! Regards Rob W. ________________________________________________ This message was sent using Dodo Webmail - www.dodo.com.au From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Apr 17 01:41:46 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Apr 17 01:41:47 2007 Subject: [Histonet] Zinc Formalin and Iron stains Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124755A@wahtntex2.waht.swest.nhs.uk> Zinc also mordents haematoxylin resulting in a very intense nuclear stain; makes tissue brittle too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The bond that links your true family is not one of blood, but of respect and joy in each other's life. Rarely do members of one family grow up under the same roof. --Richard Bach This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From yvan_lindekens <@t> yahoo.com Tue Apr 17 05:06:44 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Apr 17 05:06:52 2007 Subject: [Histonet] Re: Picric acid Message-ID: <896411.44094.qm@web30910.mail.mud.yahoo.com> There were some problems in a high school in the city of Sint-Niklaas, Belgium too, in september last year. 1400 students were evacuated after which the bomb squad took the picric acid away and detonated it. According to Belgian newspapers it left a crater of one meter in diameter. I wonder what charge they used to detonate it... According to the science teacher who found the bottle containing about 200gm of dried out picric acid, he wasn't shure whether it was "picric acid" or "citric acid" in the bottle... Excuse me: I wouldn't trust such a science teacher to teach my children. Anyway, it was a good story for the newspapers: "Time bomb thicking in high school", "Our children in danger", "Science teacher saves more than 1000 children", "A heroe!". Perhaps a new entry for the manuals on psychopatology: "picric acid psychosis"... __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From BMolinari <@t> heart.thi.tmc.edu Tue Apr 17 05:25:47 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Apr 17 05:25:55 2007 Subject: [Histonet] Proper Decalcification In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F0F3@exchange.gmhpost.com> Message-ID: It is a digital x-ray machine. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Amy Self [mailto:ASelf@gmhsc.com] Sent: Monday, April 16, 2007 9:44 AM To: Molinari, Betsy Subject: RE: [Histonet] Proper Decalcification This is the first I have heard of this--- What is it... Thanks, amy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Molinari, Betsy Sent: Monday, April 16, 2007 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification Faxitron MX 20 Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Monday, April 16, 2007 5:47 AM To: Molinari, Betsy Subject: RE: [Histonet] Proper Decalcification Which model do you use? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, April 16, 2007 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification We use the Faxitron. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Friday, April 13, 2007 10:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Proper Decalcification I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From TillRenee <@t> uams.edu Tue Apr 17 07:31:10 2007 From: TillRenee <@t> uams.edu (Till, Renee) Date: Tue Apr 17 07:31:38 2007 Subject: [Histonet] Old paraffin sections Message-ID: <11F927674DEBDC43B960809A7403C5D204144125@MAILPED.ad.uams.edu> What is your opinion of the effectiveness of IHC on paraffin sections that have not been recently cut? I have been trying to do a PCNA on some liver paraffin sections. I was given the paraffin blocks, but also some slides boxes of previously cut slides from 1 to 2 years old. Do you think this could greatly effect the staining of the slides? I don't normally do too much liver staining, so I had begun adapting my mammary gland PCNA protocol to the livers, when I was given a protocol this group had previously used in several of their papers. When I try to duplicate this protocol, I get faint staining to negative slides. I have tried everything I can think of. Remaking solutions, using a newer secondary in case the other had been frozen too long (though I've never had a problem with that before), leaving the DAB on longer, anything that still is within the protocol. The only thing left I can think of is the age of the sections. The only other thing I can think of is that my secondary is from a different company than theirs, and I can't do anything about that short of ordering the other and waiting for it. I generally order my secondaries from Jackson and have never had any problems with them. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JWEEMS <@t> sjha.org Tue Apr 17 07:35:42 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 17 07:36:17 2007 Subject: [Histonet] Picric acid In-Reply-To: <000b01c7806b$63035fd0$6500a8c0@mainbox> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9516@sjhaexc02.sjha.org> You would put the bottle in a bucket of water and open it under water. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bryan Hewlett Sent: Monday, April 16, 2007 5:09 PM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Picric acid How would you get the bottle open to add the water??? Moving the bottle or opening it could set it off. Dumb move!!! Bryan ----- Original Message ----- From: To: Sent: Monday, April 16, 2007 4:32 PM Subject: [Histonet] Picric acid > It probably would have been easier to add some water to the > bottle......and > less expensive too. > > > > > ************************************** See what's free at > http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Tue Apr 17 07:43:10 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 17 07:43:35 2007 Subject: [Histonet] Picric acid In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9516@sjhaexc02.sjha.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9519@sjhaexc02.sjha.org> oops I meant to put a ? after this sentence... would that work? Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Tuesday, April 17, 2007 8:36 AM To: Bryan Hewlett; AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Picric acid You would put the bottle in a bucket of water and open it under water. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bryan Hewlett Sent: Monday, April 16, 2007 5:09 PM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Picric acid How would you get the bottle open to add the water??? Moving the bottle or opening it could set it off. Dumb move!!! Bryan ----- Original Message ----- From: To: Sent: Monday, April 16, 2007 4:32 PM Subject: [Histonet] Picric acid > It probably would have been easier to add some water to the > bottle......and > less expensive too. > > > > > ************************************** See what's free at > http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Jackie.O'Connor <@t> abbott.com Tue Apr 17 07:54:09 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Apr 17 07:54:46 2007 Subject: [Histonet] Re: Picric acid - Science teachers In-Reply-To: <896411.44094.qm@web30910.mail.mud.yahoo.com> Message-ID: Speaking of good v.bad science teachers. In grammar school (at about the age of 8 years) my daughter brought home a paper she was very proud of. She was asked to draw a picture of a vertebrate and a non-vertebrate. She drew a picture of a donkey and a hamster - - and was given a grade of 100% correct. I brought this up to the teacher who told me that it WAS correct because since rodents have no spines in order to fit under doorways, they are - in her opinion - invertebrates. My daughter has since graduated from DePaul University with a biology degree - - I dont' know where that teacher went to school. Jackie O' yvan lindekens Sent by: histonet-bounces@lists.utsouthwestern.edu 04/17/2007 05:06 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Picric acid There were some problems in a high school in the city of Sint-Niklaas, Belgium too, in september last year. 1400 students were evacuated after which the bomb squad took the picric acid away and detonated it. According to Belgian newspapers it left a crater of one meter in diameter. I wonder what charge they used to detonate it... According to the science teacher who found the bottle containing about 200gm of dried out picric acid, he wasn't shure whether it was "picric acid" or "citric acid" in the bottle... Excuse me: I wouldn't trust such a science teacher to teach my children. Anyway, it was a good story for the newspapers: "Time bomb thicking in high school", "Our children in danger", "Science teacher saves more than 1000 children", "A heroe!". Perhaps a new entry for the manuals on psychopatology: "picric acid psychosis"... __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Apr 17 07:59:41 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Apr 17 08:00:19 2007 Subject: [Histonet] Old paraffin sections In-Reply-To: <11F927674DEBDC43B960809A7403C5D204144125@MAILPED.ad.uams.edu> Message-ID: Many antigens will deteriorate on old cut paraffin sections - well documented instances are CD31, and Ki67. I would guess PCNA would be the same. "Till, Renee" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/17/2007 07:31 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Old paraffin sections What is your opinion of the effectiveness of IHC on paraffin sections that have not been recently cut? I have been trying to do a PCNA on some liver paraffin sections. I was given the paraffin blocks, but also some slides boxes of previously cut slides from 1 to 2 years old. Do you think this could greatly effect the staining of the slides? I don't normally do too much liver staining, so I had begun adapting my mammary gland PCNA protocol to the livers, when I was given a protocol this group had previously used in several of their papers. When I try to duplicate this protocol, I get faint staining to negative slides. I have tried everything I can think of. Remaking solutions, using a newer secondary in case the other had been frozen too long (though I've never had a problem with that before), leaving the DAB on longer, anything that still is within the protocol. The only thing left I can think of is the age of the sections. The only other thing I can think of is that my secondary is from a different company than theirs, and I can't do anything about that short of ordering the other and waiting for it. I generally order my secondaries from Jackson and have never had any problems with them. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raghulvet <@t> yahoo.co.in Tue Apr 17 08:22:04 2007 From: raghulvet <@t> yahoo.co.in (raghul) Date: Tue Apr 17 08:22:16 2007 Subject: [Histonet] alcian blue_ precipitation Message-ID: <99519.9722.qm@web8318.mail.in.yahoo.com> dear histonetters When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin cells in positive control. please help raghul Medbiotech res. limited chennai, India --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Apr 17 09:07:47 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Apr 17 09:07:51 2007 Subject: [Histonet] alcian blue_ precipitation Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247570@wahtntex2.waht.swest.nhs.uk> When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin cells in positive control. please help Raghul Logically if 'Alcian Blue' is not dissolving in water but does dissolve in 1:1 water:alcohol then it is the alcohol that it is dissolving in and I don't know if it works non polar liquids, but then a mixture would be polar wouldn't it? (I dunno, confused). Alcian Blue I think is usually used as an aqueous solution of 1% at various PHs. The only thing I wonder is that Alcian Blue 8GX is the usual dye used and Alcian Blue 8XM is less soluble and is used in lower concentrations. I gather the composition of Alcian Blue 8GX has, over the years, been changed to make it more soluble. These are basic dyes with a phthalocynain nucleus and basic side chain; logically you either been sold the 'wrong stuff' (8XM) or you have a poorly soluble variant. Send it back, get some more. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The bond that links your true family is not one of blood, but of respect and joy in each other's life. Rarely do members of one family grow up under the same roof. --Richard Bach This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From bhewlett <@t> cogeco.ca Tue Apr 17 09:08:55 2007 From: bhewlett <@t> cogeco.ca (bhewlett@cogeco.ca) Date: Tue Apr 17 09:09:03 2007 Subject: [Histonet] alcian blue_ precipitation Message-ID: <4624d4f7.2cd.68cb.17109@cogeco.ca> > Raghul, try dissolving the Alcian blue powder in distilled water first, then adjust the pH with acid. You should have no problem with pH 2.5, but some lot #s of dye will precipitate at pH 1.0. If this happens contact me again. Bryan > dear histonetters > > When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting > dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin > cells in positive control. please help > > raghul > Medbiotech res. limited > chennai, India > > > --------------------------------- > Check out what you're missing if you're not on Yahoo! Messenger > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Tue Apr 17 09:14:50 2007 From: bhewlett <@t> cogeco.ca (bhewlett@cogeco.ca) Date: Tue Apr 17 09:14:58 2007 Subject: [Histonet] Picric acid Message-ID: <4624d65a.312.6e42.15002@cogeco.ca> > You still have the problem of moving a potentially explosive bottle!! Still a dumb move, call the bomb disposal experts. > You would put the bottle in a bucket of water and open it under water.Joyce WeemsPathology ManagerSaint Joseph's > Hospital 5665 Peachtree Dunwoody Rd NEAtlanta, GA 30342404-851-7376 - Phone404-851-7831 - Fax-----Original > Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf > Of BryanHewlettSent: Monday, April 16, 2007 5:09 PMTo: AGrobe2555@aol.com; histonet@lists.utsouthwestern.eduSubject: > Re: [Histonet] Picric acidHow would you get the bottle open to add the water???Moving the bottle or opening it could > set it off.Dumb move!!!Bryan----- Original Message ----- From: To: > Sent: Monday, April 16, 2007 4:32 PMSubject: [Histonet] Picric acid> It probably > would have been easier to add some water to the > bottle......and> less expensive too.>>>>> > ************************************** See what's free at > http://www.aol.com.> > _______________________________________________> Histonet mailing list> H istonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________Histonet > mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetConfidentiality > Notice ** The information contained in this message may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by replying to the message and deleting it from > your computer. Thank you. Saint Joseph's Health System, Inc. From cmiller <@t> physlab.com Tue Apr 17 09:45:24 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Apr 17 09:45:34 2007 Subject: [Histonet] Picric acid In-Reply-To: <4624d65a.312.6e42.15002@cogeco.ca> References: <4624d65a.312.6e42.15002@cogeco.ca> Message-ID: <001701c780ff$081ecac0$db01a8c0@plab.local> As a new supervisor and new employee, I just last month had the bomb Squad remove a very dry jar of picric acid 25 yrs old! And it did go boom when they detonated it. I was happy to discover it was also free of charge. Nice to know those high taxes pay for more than street repair!!( chuck hole city) Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bhewlett@cogeco.ca Sent: Tuesday, April 17, 2007 9:15 AM To: Weems, Joyce; Bryan Hewlett; AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Picric acid > You still have the problem of moving a potentially explosive bottle!! Still a dumb move, call the bomb disposal experts. > You would put the bottle in a bucket of water and open it under water.Joyce WeemsPathology ManagerSaint Joseph's > Hospital 5665 Peachtree Dunwoody Rd NEAtlanta, GA 30342404-851-7376 - Phone404-851-7831 - Fax-----Original > Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utso uthwestern.edu]On Behalf > Of BryanHewlettSent: Monday, April 16, 2007 5:09 PMTo: AGrobe2555@aol.com; histonet@lists.utsouthwestern.eduSubject: > Re: [Histonet] Picric acidHow would you get the bottle open to add the water???Moving the bottle or opening it could > set it off.Dumb move!!!Bryan----- Original Message ----- From: To: > Sent: Monday, April 16, 2007 4:32 PMSubject: [Histonet] Picric acid> It probably > would have been easier to add some water to the > bottle......and> less expensive too.>>>>> > ************************************** See what's free at > http://www.aol.com.> > _______________________________________________> Histonet mailing list> H istonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________Histonet > mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman /listinfo/histonetConfidentiality > Notice ** The information contained in this message may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by replying to the message and deleting it from > your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Tue Apr 17 09:51:03 2007 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Tue Apr 17 09:51:49 2007 Subject: [Histonet] HT needed Message-ID: <46249887020000C50000D476@MAIL.TSRH.ORG> Texas Scottish Rite Hospital - Histotechnologist Texas Scottish Rite Hospital is looking for a qualified Histotechnologist that will be responsible for supporting research projects in tissue preparation, microtomy, staining, mounting, special procedures, histochemistry, hard tissue histology and image analysis of pathological specimens. BS in Medical Technology/Histology or BS in Biomedical Sciences required. HT (ASCP) or eligible preferred with min 5 yrs work exp. Visit www.tsrhc.org for more information. Please submit resumes to tsrhhr@tsrh.org or via fax at 214-559-7595. ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From stamptrain <@t> yahoo.com Tue Apr 17 09:53:51 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Tue Apr 17 09:53:56 2007 Subject: [Histonet] Picric acid In-Reply-To: <4624d65a.312.6e42.15002@cogeco.ca> Message-ID: <597520.66854.qm@web55814.mail.re3.yahoo.com> I have to chime in on this. I had an experience back in the olde tymes (at least 25-30 yrs ago!) when I had to clean out an old stock area. I had a chemist look through to make sure I had nothing nasty in there (the chemicals were from a pathologist who did his own thing--started in the 30's) and then had a summer intern start hauling cartloads to the lab where we sorted things out and disposed of them appropriately. Well, what comes out on one bumpy cartload but a nearly full bottle of Picric Acid all recrystallized into beautiful orange crystals on the lid and throughout the jar. It (and we) survived the bumpy ride (so much for having a chemist check things out for nasty substances), and I carefully took the jar, placed it in the back of a hood, and notified the proper authorities (Safety, of all people). About one hour later we were evacuating the building (it was a beautiful, warm May afternoon, and the ice cream truck stopped by...but that's another story) while the bomb squad gingerly removed the offending bottle and disposed of it (I think they eventually detonated it, but there was no big bang). Then, the facility director (a pathologist) had me accompany him on a sweep through the building for any other possible explosives (what am I, a bomb squad member here?) during which sweep we found a brand new bottle (received the day before) of Picric Acid (with the requisite water) in the Histology Lab (of all places). Well, in comes the Bomb Squad, out goes the Picric Acid and the Histologists land all over me. At least the ice cream was good....;-) Roger Moretz, Ph.D. from another place than where the Picric Acid incident took place. --- bhewlett@cogeco.ca wrote: > > You still have the problem of moving a potentially > explosive bottle!! > Still a dumb move, call the bomb disposal experts. > > > You would put the bottle in a bucket of water and > open it under water.Joyce WeemsPathology > ManagerSaint Joseph's > > Hospital 5665 Peachtree Dunwoody Rd NEAtlanta, GA > 30342404-851-7376 - Phone404-851-7831 - > Fax-----Original > > Message-----From: > histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On > Behalf > > Of BryanHewlettSent: Monday, April 16, 2007 5:09 > PMTo: AGrobe2555@aol.com; > histonet@lists.utsouthwestern.eduSubject: > > Re: [Histonet] Picric acidHow would you get the > bottle open to add the water???Moving the bottle or > opening it could > > set it off.Dumb move!!!Bryan----- Original Message > ----- From: To: > > Sent: Monday, > April 16, 2007 4:32 PMSubject: [Histonet] Picric > acid> It probably > > would have been easier to add some water to the > > bottle......and> less expensive too.>>>>> > > ************************************** See what's > free at > http://www.aol.com.> > > _______________________________________________> > Histonet mailing list> H > istonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________Histonet > > mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetConfidentiality > > Notice ** The information contained in this > message may be privileged and is confidential > information intended for the > > use of the addressee listed above. If you are > neither the intended recipient nor the employee or > agent responsible for > > delivering this message to the intended recipient, > you are hereby notified that any disclosure, > copying, distribution > > or the taking of any action in reliance on the > contents of this information is strictly prohibited. > If you have > > received this communication in error, please > notify us immediately by replying to the message and > deleting it from > > your computer. Thank you. Saint Joseph's Health > System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Eric.C.Kellar <@t> questdiagnostics.com Tue Apr 17 10:04:47 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Tue Apr 17 10:05:07 2007 Subject: [Histonet] alcian blue_ precipitation Message-ID: <6843061CE6B98E4B96590D4F299618F801583C1C@qdcws0117.us.qdx.com> The dye required is Alcian Blue 8G. Alcian Blue 5G and 7G are similar, but carry fewer of the cationic substituents and are less water soluble. Alcian Blue 8GX (X for extra) has a higher dye content than does 8GS (S for standard). The stability of the dye is variable. Depending on the age of your dye lot, it could take approximately 1 hour to dissolve Alcian Blue 8GX into aqueous solution with a magnetic stirrer, then adjust the pH. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Tuesday, April 17, 2007 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_ precipitation dear histonetters When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin cells in positive control. please help raghul Medbiotech res. limited chennai, India --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From gcallis <@t> montana.edu Tue Apr 17 10:08:37 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 17 10:08:21 2007 Subject: [Histonet] Zinc Formalin fixation In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0124755A@wahtntex2.waht.sw est.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0124755A@wahtntex2.waht.swest.nhs.uk> Message-ID: <6.0.0.22.1.20070417090357.01b58d48@gemini.msu.montana.edu> We never had a problem with tissue being brittle after fixation with the commercial Anatech zinc formalin (buffered) although I have heard others complain about this along with Liz's comments about precipitation problems, maybe in house prepared zinc formalin? However, the zinc TRIS buffer fixative, that is FORMALIN FREE, for CD marker immunohistochemistry (Beckstead, J Histochem Cytochem) made tissues terrible brittle. At 12:41 AM 4/17/2007, you wrote: >Zinc also mordents haematoxylin resulting in a very intense nuclear >stain; makes tissue brittle too. > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Tue Apr 17 10:17:56 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 17 10:17:39 2007 Subject: [Histonet] alcian blue_ precipitation In-Reply-To: <99519.9722.qm@web8318.mail.in.yahoo.com> References: <99519.9722.qm@web8318.mail.in.yahoo.com> Message-ID: <6.0.0.22.1.20070417091406.01b53028@gemini.msu.montana.edu> Our pH 2.5 alcian blue was dissolved in 100 ml of 3% glacial acetic acid, and pH 1 alcian blue was dissolved in 0.1N HCL. Your alcian blue may be old? Or not biological stain commission certified. We also store our alcian blue in the refrigerator, per recommendation of R.W. Mowry of McManus and Mowry fame, his lecture at NSH some years ago. At 07:22 AM 4/17/2007, you wrote: >dear histonetters > > When i try to dissolve the alcian blue (sigma) powder in acidified > (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 > alchol:distilled water adjusted to required pH its dissolving but isnt > staining any of mucin cells in positive control. please help > > raghul > Medbiotech res. limited > chennai, India > > >--------------------------------- > Check out what you're missing if you're not on Yahoo! Messenger >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From akemiat3377 <@t> yahoo.com Tue Apr 17 10:17:34 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Apr 17 10:17:44 2007 Subject: [Histonet] alcian blue_ precipitation In-Reply-To: <99519.9722.qm@web8318.mail.in.yahoo.com> Message-ID: <240166.70643.qm@web31304.mail.mud.yahoo.com> This is what I do with my Alcian Blue. My stabalizer is missing from this protocol because it is proprietary. By the way, Anatech has the best Alcian Blue on the market! The most dye content!!! 1. Label the appropriately sized container with description, lot number, storage conditions, expiration date, prepared by and date. Add a large stir bar to the container. 2. Measure the 3% acetic acid solution determined in the Calculation section of Manufacturing Document. Transfer the 3% Acetic Acid to the labeled container, and begin stirring on a large stir plate. Add the Alcian Blue and continue mixing solution. 3. Mix thoroughly for 30?5 minutes or until all the chemicals and reagents have dissolved. Visually inspect solution to ensure adequate mixing. 4. Measure the pH of the solution using a pH meter calibrated at pH 1 and pH 4. The pH must be 2.5 ? 0.1. If necessary, adjust pH with NaOH of HCL. 5. The reagent is stable for 12 months at room temperature. Store the reagent at room temperature in an opaque container. 6. Test for quality control. Good Luck, Akemi Allison-Tacha --- raghul wrote: > dear histonetters > > When i try to dissolve the alcian blue (sigma) > powder in acidified (ph2.5/ph1.0) dd water the stain > isnt getting dissolved. When i try 1:1 > alchol:distilled water adjusted to required pH its > dissolving but isnt staining any of mucin cells in > positive control. please help > > raghul > Medbiotech res. limited > chennai, India > > > --------------------------------- > Check out what you're missing if you're not on > Yahoo! Messenger > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ElizabethWyand <@t> texashealth.org Tue Apr 17 11:06:22 2007 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Tue Apr 17 11:06:30 2007 Subject: [Histonet] HT positions Message-ID: <26BE9ACC202D29479B0A144066A733E5022404DA@phdex01.txhealth.org> MD Pathology currently has openings for 2 certified Histotechnologists. One is for hours 10pm-6am (somewhat negotiable) and the second is PT/PRN from 2am-8am. MD Pathology is a private anatomic lab located in Plano and offers very competitive wages and benefits for full times employees. Please submit resumes to geniejacobs@texashealth.org or via fax to 972-981-3236. The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From PMonfils <@t> Lifespan.org Tue Apr 17 11:29:18 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Apr 17 11:29:23 2007 Subject: [Histonet] propidium iodide protocol In-Reply-To: <5f4fef8e23ad.462392c1@med.cornell.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C69@LSRIEXCH1.lsmaster.lifespan.org> Are you doing this assessment visually, or by flow cytometry? In either case, propidium iodide wouldn't be my first choice for assessing viability. It is quite cytotoxic, and unless you keep the concentration very low it will kill the cells. It also requires use of a fluorescence microscope. One of the classic methods like trypan blue staining would be my preference for visual assessment. It has low toxicity and can be viewed with a standard light microscope. If you do want to do the assessment visually by fluorescence, fluorescein diacetate would be my first choice. It's very fast acting and is not cytotoxic. For flow cytometric assessment PI can be used successfully if you keep the concentration very low. This is not a problem in flow cytometry because the cytometer is much more sensitive to low level fluorescence emission than the human eye is. But again, I find fluorescein diacetate a more reliable method for assessing viability by flow cytometry, for the same reasons mentioned above. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of William Ares > Sent: Monday, April 16, 2007 3:14 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] propidium iodide protocol > > Does anyone have a protocol that they can share for use of propidium iodide to assess cell death in culture (medulloblastoma culture, if that makes a difference)? I'm using both 96 well plates and 35ml flasks and Sigma Aldrich's Propidium Iodide Solution. Thanks. > > > William Ares > Research Technician > Laboratory of Molecular and Developmental Neuroscience > Weill Medical College of Cornell University > Tel: (212) 746 5056 > Email: wia2005@med.cornell.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From akemiat3377 <@t> yahoo.com Tue Apr 17 13:19:13 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Apr 17 13:19:19 2007 Subject: [Histonet] Alcian Blue 8GX and S from someone who made it to sell Message-ID: <483244.40926.qm@web31304.mail.mud.yahoo.com> FYI to those of you who are not in the loop. Alcian Blue has not been procured in it's natural form for several years. There were too many fatalities during it's procurement from South America. This came to my attention in the late 90's. Anatech started creating it, in a synthetic form some years ago. It was my understanding and I could be wrong, that for awhile, Anatech was the only company who produced Alcian Blue to pass the Biological Stains Commission evaluation process. It is my understanding, there is only a 8GS being made now. Again, I could be wrong. In the past few years, Dudley Chemical, Aldrich, Sigma, EM Science and some Chinese companies were able to produce synthetic Alcian Blue, although the dye content varies with each companies submission. You should always get a seal from the Biological Stains Commission on each bottle of Alcian Blue powder. If the label is not on it, it has not passed their evaluation process. This only means that it met it's minimum standard. The Commision will not divulge actual test results. When I started my R&D on Alcian Blue for Biocare, Anatech had the highest dye content from the few companies that sold it. It is only my opinion, but they still have the best on the market. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com From fetid <@t> hotmail.com Tue Apr 17 13:41:05 2007 From: fetid <@t> hotmail.com (Jaume del Valle) Date: Tue Apr 17 13:41:10 2007 Subject: [Histonet] Evans Blue Message-ID: Dear Histomembers Some months ago I've trying to work with Evans blue. My problem is that I inject it (IV or IP) and when I want to see the fluorescence of the dye in the brain there is a lot of... Of red signal here and there. I've read that the tissue have to be fixed (which fixative is the best? At which temperature? How much time? Do I have to let the sample to air-dry? Some minutes? Hours? The animal have to be perfused? With PFA? Saline is OK? When I let to dry at Room temperature the signal spreads all over the sample, when I use Mowiol to Mount the slide it seems that the evans blue gets disolved... Have anybody worked with Evans blue and Brain slides to see extravastion? Please, give me the (whole) details!!! Jaume del Valle Universitat de Barcelona _________________________________________________________________ Consigue el nuevo Windows Live Messenger http://get.live.com/messenger/overview From bhewlett <@t> cogeco.ca Tue Apr 17 13:50:28 2007 From: bhewlett <@t> cogeco.ca (bhewlett@cogeco.ca) Date: Tue Apr 17 13:50:32 2007 Subject: [Histonet] Alcian Blue 8GX and S from someone who made it to sell Message-ID: <462516f4.38f.89e.24449@cogeco.ca> > Akemi, Alcian blue was NEVER available in any natural form. It is and always has been, a synthetic dye first produced by ICI in the early fifties. The process of production required some dangerous intermediates, that was why production ceased. Production now involves less dangerous intermediates and hence more expense(naturally). Bryan > FYI to those of you who are not in the loop. Alcian > Blue has not been procured in it's natural form for > several years. There were too many fatalities during > it's procurement from South America. This came to my > attention in the late 90's. > > Anatech started creating it, in a synthetic form some > years ago. It was my understanding and I could be > wrong, that for awhile, Anatech was the only company > who produced Alcian Blue to pass the Biological Stains > Commission evaluation process. It is my understanding, > there is only a 8GS being made now. Again, I could be > wrong. > > In the past few years, Dudley Chemical, Aldrich, > Sigma, EM Science and some Chinese companies were able > to produce synthetic Alcian Blue, although the dye > content varies with each companies submission. You > should always get a seal from the Biological Stains > Commission on each bottle of Alcian Blue powder. If > the label is not on it, it has not passed their > evaluation process. This only means that it met it's > minimum standard. The Commision will not divulge > actual test results. > > When I started my R&D on Alcian Blue for Biocare, > Anatech had the highest dye content from the few > companies that sold it. It is only my opinion, but > they still have the best on the market. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology, SS, IHC, & Microarray > Madison, WI > Tele: (925) 788-0900 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Apr 17 14:09:14 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Apr 17 14:09:17 2007 Subject: [Histonet] Alcian Blue 8GX and S from someone who made it to sell In-Reply-To: <462516f4.38f.89e.24449@cogeco.ca> Message-ID: <168363.73522.qm@web31315.mail.mud.yahoo.com> Well, I stand corrected! Never let it be said that I don't admit when I am wrong. This was what was stated to me. I always say, The mind is like a parachute, keep it open! By the way, the Alcian Blue pH 2.5/PAS from Biocare's website still has the hematoxylin showing nicely. That image was taken prior to incorporating any stabalizers in the stains. I'm not sure if it is still on the website. I know that several stain kits have been eliminated, since I left. No $$ in it compared to IHC. They no longer have ART Names for the kit's, just the generic name. Also, FYI--I don't get any residuals from Biocare. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- bhewlett@cogeco.ca wrote: > > Akemi, > > Alcian blue was NEVER available in any natural form. > It is and always has been, a synthetic dye first > produced by ICI in the early fifties. The process of > production > required some dangerous intermediates, that was why > production ceased. Production now involves less > dangerous > intermediates and hence more expense(naturally). > > Bryan > > > > FYI to those of you who are not in the loop. > Alcian > > Blue has not been procured in it's natural form > for > > several years. There were too many fatalities > during > > it's procurement from South America. This came to > my > > attention in the late 90's. > > > > Anatech started creating it, in a synthetic form > some > > years ago. It was my understanding and I could be > > wrong, that for awhile, Anatech was the only > company > > who produced Alcian Blue to pass the Biological > Stains > > Commission evaluation process. It is my > understanding, > > there is only a 8GS being made now. Again, I > could be > > wrong. > > > > In the past few years, Dudley Chemical, Aldrich, > > Sigma, EM Science and some Chinese companies were > able > > to produce synthetic Alcian Blue, although the dye > > content varies with each companies submission. You > > should always get a seal from the Biological > Stains > > Commission on each bottle of Alcian Blue powder. > If > > the label is not on it, it has not passed their > > evaluation process. This only means that it met > it's > > minimum standard. The Commision will not divulge > > actual test results. > > > > When I started my R&D on Alcian Blue for Biocare, > > Anatech had the highest dye content from the few > > companies that sold it. It is only my opinion, > but > > they still have the best on the market. > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > President > > Phoenix Lab Consulting & Staffing > > Specializing in Histology, SS, IHC, & Microarray > > Madison, WI > > Tele: (925) 788-0900 > > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Tue Apr 17 14:24:36 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Apr 17 14:24:41 2007 Subject: [Histonet] Alcian Blue Stabalizer Message-ID: <665144.56148.qm@web31314.mail.mud.yahoo.com> --- Akemi Allison-Tacha wrote: > Date: Tue, 17 Apr 2007 12:22:07 -0700 (PDT) > From: Akemi Allison-Tacha > Subject: RE: [Histonet] Alcian Blue 8GX and S from > someone who made it to sell > To: Sarah Jones > > Sarah, I am speaking about incorporating a post > stabalizer it the lq.solution of Alcian Blue not the > synthetic powder dye. > > Akemi > > --- Sarah Jones wrote: > > > John A. Kiernan was my basic source for the > research > > I did recently on > > Alcian Blue. In his book, Histological & > > Histochemical Methods, he > > states (referring to Alcian Blue 8GS, 8GX or 8GN), > > "All these dyes are > > supplied mixed with boric acid, sodium sulphate, > and > > dextrin." Also, in > > an e-mail to the Histonet, Kiernan writes: "A low > > dye content such as > > the 10-20% in your old ICI material is not > > necessarily a bad thing for > > Alcian Blue, and batches with high dye content > > (70%+) often perform > > badly. It seems likely that the additives > (dextrin, > > boric acid, etc.) > > are needed in adequate amount." In addition, > > quoting from Churukian et > > al, 1972: "Minimum dye content for Commission > > certification is 50%. A > > high dye content is at the expense of less > > stabilizer. Consequently, > > purer samples may have reduced shelf-lives." > > > > Sarah Jones > > > > > > > > -----Original Message----- > > From: Akemi Allison-Tacha > > [mailto:akemiat3377@yahoo.com] > > Sent: Tuesday, April 17, 2007 11:49 AM > > To: Sarah Jones > > Subject: RE: [Histonet] Alcian Blue 8GX and S from > > someone who made it > > to sell > > > > Very Interesting. Chuck Churukian does not > mention > > any stabilizers in > > his Special Stains book when making it up. He was > > in charge of > > evaluating all dyes for the Biological Stains > > Commission before > > semi-retiring. > > We spoke on several collaborations for Special > > Stains and he was one of > > the people who the CAT hematoxylin > > was named after. > > > > The image that is on the www.biocare.net site for > > Alcian Blue pH 2.5 was > > taken w/o the stabilizer. I only started putting > in > > a stabilizer after > > we started to sell it. > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > President > > Phoenix Lab Consulting & Staffing > > Specializing in Histology, SS, IHC, & Microarray > > Madison, WI > > Tele: (925) 788-0900 > > E-Mail: akemiat3377@yahoo.com > > > > > > --- Sarah Jones wrote: > > > > > Unfortunately, with Alcian Blue a higher dye > > content doesn't > > > necessarily mean a better dye. Alcian Blue > > absolutely NEEDS the > > > stabilizers in order to work properly. The > higher > > the dye content, > > > the fewer stabilizers are in the dye. This is > an > > exception to the > > > usual rule about dyes and high dye content. > > > Personally, I am finding that the synthetic > Alcian > > Blue dye renders a > > > different blue than the Robin's egg blue we were > > used to seeing over > > > the years. This blue seems to disappear if you > > use a Hematoxylin > > > counterstain, such as in the Alcian > > Blue/PAS-Hematoxylin. > > > > > > Sarah Jones > > > Dako North America > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > > On Behalf Of Akemi > > > Allison-Tacha > > > Sent: Tuesday, April 17, 2007 11:19 AM > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Alcian Blue 8GX and S from > > someone who made it to > > > sell > > > > > > FYI to those of you who are not in the loop. > > Alcian Blue has not been > > > procured in it's natural form for several years. > > > There were too many > > > fatalities during it's procurement from South > > America. This came to > > > my attention in the late 90's. > > > > > > Anatech started creating it, in a synthetic form > > some years ago. It > > > was my understanding and I could be wrong, that > > for awhile, Anatech > > > was the only company who produced Alcian Blue to > > pass the Biological > > > Stains Commission evaluation process. It is my > > understanding, there is > > > only a 8GS being made now. Again, I could be > > wrong. > > > > > > In the past few years, Dudley Chemical, Aldrich, > > Sigma, EM Science and > > > some Chinese companies were able to produce > > synthetic Alcian Blue, > > > although the dye content varies with each > > companies submission. You > > > should always get a seal from the Biological > > Stains Commission on each > > > bottle of Alcian Blue powder. If the label is > not > > on it, it has not > > > passed their evaluation process. This only means > > that it met it's > > > minimum standard. The Commision will not > divulge > > actual test results. > > > > > > When I started my R&D on Alcian Blue for > Biocare, > > Anatech had the > > > highest dye content from the few companies that > > sold it. It is only > > > my opinion, but they still have the best on the > > market. > > > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > > President > > > Phoenix Lab Consulting & Staffing > > > Specializing in Histology, SS, IHC, & Microarray > > Madison, WI > > > Tele: (925) 788-0900 > > > E-Mail: akemiat3377@yahoo.com > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > From ASelf <@t> gmhsc.com Tue Apr 17 14:33:05 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Tue Apr 17 14:33:00 2007 Subject: [Histonet] Processing Specimens Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F0F9@exchange.gmhpost.com> Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From LRaff <@t> lab.uropartners.com Tue Apr 17 14:35:29 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Tue Apr 17 14:35:13 2007 Subject: [Histonet] Inking of same type specimens Message-ID: <5DA1CA5D0B98A84985B545A24423B822052E84@UPLAB01.uplab.local> Are any of you following the trend of inking cores from similar types cases with different colors to prevent misidentification? For example, prostate biopsies from patient A are inked green, prostate biopsies from patient B inked red, prostate biopsies from patient C inked blue, and then back to green for prostate patient D. If anyone is doing this: 1. How long have you been doing it 2. Does it significantly impact grossing time 3. What method are you using for inking (the little dropper bottles, big bottles with disposable pipettes, other) 4. Are you diluting the ink 5. Does it make a mess 6. Have you found it worthwhile Thanks! Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From rjbuesa <@t> yahoo.com Tue Apr 17 14:39:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 17 14:39:17 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F0F9@exchange.gmhpost.com> Message-ID: <747918.7942.qm@web61223.mail.yahoo.com> Amy: The "clue" for the answer is in the "NOTE". If a pathologist is doing the tissue processing (TP) (which they don't), then don't mind to answers. IF, the TP is done by the histotechs (which they do) the protocol has to be in the SOP and the answer would be: "described in the SOP". Ren? J. Amy Self wrote: Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Tue Apr 17 14:45:02 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 17 14:45:09 2007 Subject: [Histonet] Inking of same type specimens In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822052E84@UPLAB01.uplab.local> Message-ID: <739132.76201.qm@web61216.mail.yahoo.com> I never saw the need for inking. Each case has its own identifying number and cassette and there was no reason for missidentifying one with any other. Ren? J. Lester Raff wrote: Are any of you following the trend of inking cores from similar types cases with different colors to prevent misidentification? For example, prostate biopsies from patient A are inked green, prostate biopsies from patient B inked red, prostate biopsies from patient C inked blue, and then back to green for prostate patient D. If anyone is doing this: 1. How long have you been doing it 2. Does it significantly impact grossing time 3. What method are you using for inking (the little dropper bottles, big bottles with disposable pipettes, other) 4. Are you diluting the ink 5. Does it make a mess 6. Have you found it worthwhile Thanks! Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From doug <@t> ppspath.com Tue Apr 17 15:51:10 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Apr 17 14:49:34 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F0F9@exchange.gmhpost.com> Message-ID: Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Apr 17 14:52:33 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 17 14:52:58 2007 Subject: [Histonet] Inking of same type specimens In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822052E84@UPLAB01.uplab.local> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9550@sjhaexc02.sjha.org> We started a couple of years ago, but found the inking to be a mess and very time consuming. The gross tech at the time "created" an agar based marker that can be cut into small pieces. He has since left, taking his formula with him. The techs have recreated something similar using agar and Histogel, acetic acid and tissue dye. We put a piece of that in each block - it embeds and cuts like tissue and still differentiates the cases. The pathologists are very happy with this method. We use it for breast, prostate, hearts, etc... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lester Raff Sent: Tuesday, April 17, 2007 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inking of same type specimens Are any of you following the trend of inking cores from similar types cases with different colors to prevent misidentification? For example, prostate biopsies from patient A are inked green, prostate biopsies from patient B inked red, prostate biopsies from patient C inked blue, and then back to green for prostate patient D. If anyone is doing this: 1. How long have you been doing it 2. Does it significantly impact grossing time 3. What method are you using for inking (the little dropper bottles, big bottles with disposable pipettes, other) 4. Are you diluting the ink 5. Does it make a mess 6. Have you found it worthwhile Thanks! Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From akemiat3377 <@t> yahoo.com Tue Apr 17 15:03:04 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Apr 17 15:03:08 2007 Subject: [Histonet] Alcian Blue Stabilizer & Manufacturing In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB301023160@exbackca.caus.dako.net> Message-ID: <727936.56337.qm@web31303.mail.mud.yahoo.com> Sarah, Perhaps, I was misunderstood. I am speaking about incorporating a post stabilizer to the lq.solution of Alcian Blue, not the synthetic powder dye. That is what I am referring to for all the previous correspondence. Below was my protocol for making-up Alcian Blue pH 2.5 w/o a post stabilizer. And by the way, alot has changed in Chuck's thoughts of special stains since 1972. I am BCC'ing him this e-mail. Believe it or not, in the beginning of my Biocare association, I actually made all the IHC products, H&E's, special stain solutions, QC'd them and bottled them. I learned alot about bulk manufacturing and pH'ing. This is what I do with my Alcian Blue. My "post" stabilizer is missing from this protocol, because it is proprietary. By the way, Anatech has the best Alcian Blue powder on the market! The best dye content! 1. Label the appropriately sized container with description, lot number, storage conditions, expiration date, prepared by and date. Add a large stir bar to the container. 2. Measure the 3% acetic acid solution determined in the Calculation section of Manufacturing Document. Transfer the 3% Acetic Acid to the labeled container, and begin stirring on a large stir plate. Add the Alcian Blue and continue mixing solution. 3. Mix thoroughly for 30?5 minutes or until all the chemicals and reagents have dissolved. Visually inspect solution to ensure adequate mixing. 4. Measure the pH of the solution using a pH meter calibrated at pH 1 and pH 4. The pH must be 2.5 ? 0.1. If necessary, adjust pH with NaOH of HCL. 5. The reagent is stable for 12 months at room temperature. Store the reagent at room temperature in an opaque container. 6. Test for quality control. > > Akemi > --- Sarah Jones wrote: > John A. Kiernan was my basic source for the research > I did recently on > Alcian Blue. In his book, Histological & > Histochemical Methods, he > states (referring to Alcian Blue 8GS, 8GX or 8GN), > "All these dyes are > supplied mixed with boric acid, sodium sulphate, and > dextrin." Also, in > an e-mail to the Histonet, Kiernan writes: "A low > dye content such as > the 10-20% in your old ICI material is not > necessarily a bad thing for > Alcian Blue, and batches with high dye content > (70%+) often perform > badly. It seems likely that the additives (dextrin, > boric acid, etc.) > are needed in adequate amount." In addition, > quoting from Churukian et > al, 1972: "Minimum dye content for Commission > certification is 50%. A > high dye content is at the expense of less > stabilizer. Consequently, > purer samples may have reduced shelf-lives." > > Sarah Jones > > > > -----Original Message----- > From: Akemi Allison-Tacha > [mailto:akemiat3377@yahoo.com] > Sent: Tuesday, April 17, 2007 11:49 AM > To: Sarah Jones > Subject: RE: [Histonet] Alcian Blue 8GX and S from > someone who made it > to sell > > Very Interesting. Chuck Churukian does not mention > any stabilizers in > his Special Stains book when making it up. He was > in charge of > evaluating all dyes for the Biological Stains > Commission before > semi-retiring. > We spoke on several collaborations for Special > Stains and he was one of > the people who the CAT hematoxylin > was named after. > > The image that is on the www.biocare.net site for > Alcian Blue pH 2.5 was > taken w/o the stabilizer. I only started putting in > a stabilizer after > we started to sell it. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology, SS, IHC, & Microarray > Madison, WI > Tele: (925) 788-0900 > E-Mail: akemiat3377@yahoo.com > > > --- Sarah Jones wrote: > > > Unfortunately, with Alcian Blue a higher dye > content doesn't > > necessarily mean a better dye. Alcian Blue > absolutely NEEDS the > > stabilizers in order to work properly. The higher > the dye content, > > the fewer stabilizers are in the dye. This is an > exception to the > > usual rule about dyes and high dye content. > > Personally, I am finding that the synthetic Alcian > Blue dye renders a > > different blue than the Robin's egg blue we were > used to seeing over > > the years. This blue seems to disappear if you > use a Hematoxylin > > counterstain, such as in the Alcian > Blue/PAS-Hematoxylin. > > > > Sarah Jones > > Dako North America > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of Akemi > > Allison-Tacha > > Sent: Tuesday, April 17, 2007 11:19 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Alcian Blue 8GX and S from > someone who made it to > > sell > > > > FYI to those of you who are not in the loop. > Alcian Blue has not been > > procured in it's natural form for several years. > > There were too many > > fatalities during it's procurement from South > America. This came to > > my attention in the late 90's. > > > > Anatech started creating it, in a synthetic form > some years ago. It > > was my understanding and I could be wrong, that > for awhile, Anatech > > was the only company who produced Alcian Blue to > pass the Biological > > Stains Commission evaluation process. It is my > understanding, there is > > only a 8GS being made now. Again, I could be > wrong. > > > > In the past few years, Dudley Chemical, Aldrich, > Sigma, EM Science and > > some Chinese companies were able to produce > synthetic Alcian Blue, > > although the dye content varies with each > companies submission. You > > should always get a seal from the Biological > Stains Commission on each > > bottle of Alcian Blue powder. If the label is not > on it, it has not > > passed their evaluation process. This only means > that it met it's > > minimum standard. The Commision will not divulge > actual test results. > > > > When I started my R&D on Alcian Blue for Biocare, > Anatech had the > > highest dye content from the few companies that > sold it. It is only > > my opinion, but they still have the best on the > market. > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > President > > Phoenix Lab Consulting & Staffing > > Specializing in Histology, SS, IHC, & Microarray > Madison, WI > > Tele: (925) 788-0900 > > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From AnthonyH <@t> chw.edu.au Tue Apr 17 16:44:51 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 17 16:45:02 2007 Subject: [Histonet] alcian blue_ precipitation Message-ID: Dissolve the dye in water first and then add diluted acid in water. Eg for 1% alcian blue in 2.5% acetic acid: 1g dye in 50ml distilled water. 2.5ml acetic acid in 47.5ml water. When the dye has dissolved, mix the two solutions Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Tuesday, 17 April 2007 11:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_ precipitation dear histonetters When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin cells in positive control. please help raghul Medbiotech res. limited chennai, India --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Apr 17 16:48:16 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 17 16:48:23 2007 Subject: [Histonet] alcian blue_ precipitation Message-ID: Alcian blue tetrakis (methylpyridinium) chloride (Sigma, Cat. No. A4045, 90% dye content) is a better dye to use. See "Improved demonstration of mast cells using alcian blue tetrakis (methylpyridium) chloride" Biotechnic & Histochemistry 77(2):93-94.(2002) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bhewlett@cogeco.ca Sent: Wednesday, 18 April 2007 12:09 AM To: raghul; histonet@lists.utsouthwestern.edu Subject: re: [Histonet] alcian blue_ precipitation > Raghul, try dissolving the Alcian blue powder in distilled water first, then adjust the pH with acid. You should have no problem with pH 2.5, but some lot #s of dye will precipitate at pH 1.0. If this happens contact me again. Bryan > dear histonetters > > When i try to dissolve the alcian blue (sigma) powder in acidified > (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try > 1:1 alchol:distilled water adjusted to required pH its dissolving but > isnt staining any of mucin cells in positive control. please help > > raghul > Medbiotech res. limited > chennai, India > > > --------------------------------- > Check out what you're missing if you're not on Yahoo! Messenger > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From billions <@t> public1.sz.js.cn Tue Apr 17 21:51:46 2007 From: billions <@t> public1.sz.js.cn (Sinoera Tech) Date: Tue Apr 17 21:52:17 2007 Subject: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX Message-ID: <02b101c78164$86973e40$d601a8c0@sinoera63d3596> Dear Histonetters, We are manufacturer of Alcian Yellow, Alcian Blue 8GX, Alcian Green, o-Cresolphthalein monophosphate disodium salt o-Cresolphthalein monophosphate N-methylglucammonium salt 1-Naphtholphthalein monophosphate 1-Naphtholphthalein monophosphate disodium salt Phenolphthalein disulfate tripotassium salt CAS NO.62625-16-5 3',3'',5',5''-Tetrachlorophenol-3,4,5,6-tetrabromosulfophthalein Indocyanine green IR-820 Please contact us for requirement. Kind Regards. - SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68258994 Tel: +86 512 68246939 http://www.sinoeratech.com http://www.sinoerachem.com From HornHV <@t> archildrens.org Wed Apr 18 07:29:24 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 18 07:29:37 2007 Subject: [Histonet] Inking of same type specimens In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9550@sjhaexc02.sjha.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70DC@EMAIL.archildrens.org> We don't use ink but we do use different colored cassettes for multiple cases of the same type of tissue. The color of the cassette is dictated into the gross description. We process multiple GI biopsies and this does help. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, April 17, 2007 2:53 PM To: Lester Raff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inking of same type specimens We started a couple of years ago, but found the inking to be a mess and very time consuming. The gross tech at the time "created" an agar based marker that can be cut into small pieces. He has since left, taking his formula with him. The techs have recreated something similar using agar and Histogel, acetic acid and tissue dye. We put a piece of that in each block - it embeds and cuts like tissue and still differentiates the cases. The pathologists are very happy with this method. We use it for breast, prostate, hearts, etc... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lester Raff Sent: Tuesday, April 17, 2007 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inking of same type specimens Are any of you following the trend of inking cores from similar types cases with different colors to prevent misidentification? For example, prostate biopsies from patient A are inked green, prostate biopsies from patient B inked red, prostate biopsies from patient C inked blue, and then back to green for prostate patient D. If anyone is doing this: 1. How long have you been doing it 2. Does it significantly impact grossing time 3. What method are you using for inking (the little dropper bottles, big bottles with disposable pipettes, other) 4. Are you diluting the ink 5. Does it make a mess 6. Have you found it worthwhile Thanks! Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Charles.Embrey <@t> carle.com Wed Apr 18 08:32:59 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Apr 18 08:33:08 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <20070417195313.66066203164@mx4.carle.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4C9@EXCHANGEBE1.carle.com> Amy, Douglas has explained it better than anyone could. Keep in mind that if you are also CLIA certified you have to go by their stronger policy. Under CLIA '88 any grossing (even what CAP now calls "processing") is high complexity testing and the person performing it must qualify. It is a snare that is bound to catch someone. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, April 17, 2007 3:51 PM To: 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Wed Apr 18 09:54:53 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Apr 18 08:54:45 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE4C9@EXCHANGEBE1.carle.com> Message-ID: Charles, Now we are touching on another issue about grossing. Grossing is now different from processing. "Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized." If you have to exercise judgment on what to submit then it is "grossing" or "high complexity testing". If you are submitting all of the tissue then it is "processing". Tissue "processing" can be performed according to standardized protocols. Grossing techs have to follow CLIA and all of the requirements for high complexity testing. ANP.11610 Phase II N/A YES NO If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? This includes the 60 semester hours of sciences and other courses. If you are processing then you do not have to be "CLIA eligible". For years histo techs have done "processing" and when the CLIA thing came into place they were not eligible anymore. In my opinion this change (processing) was made because labs were having a difficult time finding the new CLIA grossing techs who meet the requirements. This was forcing labs to find anyone with a BS and training them to gross. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Wednesday, April 18, 2007 8:33 AM To: Douglas D Deltour; Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, Douglas has explained it better than anyone could. Keep in mind that if you are also CLIA certified you have to go by their stronger policy. Under CLIA '88 any grossing (even what CAP now calls "processing") is high complexity testing and the person performing it must qualify. It is a snare that is bound to catch someone. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, April 17, 2007 3:51 PM To: 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lance.Erickson <@t> intermountainmail.org Wed Apr 18 09:12:26 2007 From: Lance.Erickson <@t> intermountainmail.org (Lance Erickson) Date: Wed Apr 18 09:12:45 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F0F9@exchange.gmhpost.com> Message-ID: <8B08EC394B366D4A895DD5686D6AFE4A58007D@LP-EXCHVS08.CO.IHC.COM> It seems the CAP has once again completely messed up on the wording of checklist questions and left it up to vague interpretation as to what is meant by their wording. In an unfortunate use of the word "processing" the CAP has tried to make the "processing" of tissue into the gross description of small and uncomplicated tissue types. How they came to use this word is beyond common sense as the definition of tissue processing in any AP lab has been set since the onset of the tissue processing procedure when first used. This new CAP question is referring to their definition of "processing" in the notes section of ANP 11600. The set of questions 11600-11670 is about tissue grossing and not histology tissue processing. In an apparent attempt to disregard the standard set out by CLIA '88 for qualifications of the personnel performing tissue gross, they are making this "processing" "generally limited to small specimens (skin ellipses, small biopsies, curetting, etc.)and does not require knowledge of anatomy" into something that can be done by anyone in the lab. Actual grossing is defined by CAP as "tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. If you have anyone that is grossing in small and non-complex specimens then you need procedures for this. These people according to the CAP now do not have to qualify as "high complexity testing personnel" under CLIA-88. Lance Erickson Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michelle.Perrins <@t> uct.ac.za Wed Apr 18 09:28:57 2007 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Wed Apr 18 09:29:18 2007 Subject: [Histonet] difference between.. Message-ID: <46264748.A704.0070.0@uct.ac.za> Hi What is the definition of a Laboratory Assistant and a Medical technician. Michelle From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 18 09:37:10 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 18 09:37:11 2007 Subject: [Histonet] difference between.. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124758E@wahtntex2.waht.swest.nhs.uk> What is the definition of a Laboratory Assistant and a Medical technician. Michelle In the UK a Laboratory Technician (now Biomedical Scientist) was state registered and if naughty could be thrown off; they could then come back as a Laboratory Assistant and be naughty again but this time supervised by a Biomedical Scientist who gets thrown off the register for letting the assistant be naughty. Dunno in America I guess you get sued. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The bond that links your true family is not one of blood, but of respect and joy in each other's life. Rarely do members of one family grow up under the same roof. --Richard Bach This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Charles.Embrey <@t> carle.com Wed Apr 18 09:41:48 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Apr 18 09:41:55 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <20070418135727.E786F20316C@mx4.carle.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4CA@EXCHANGEBE1.carle.com> Douglas, You are right, according to CAP. You have to remember that CLIA and CAP are separate and not interconnected with their requirements. The problem is that CLIA has not changed the standard since CLIA '88 and it doesn't look like they are about to any time soon. My lab is both CAP and CLIA certified so I have to follow the stricter rule on grossing. CAP cannot dictate to CLIA or soften CLIA standards since they are two entirely different entities (civilian vs. government). As far as CLIA '88 dictates processing techs (as outlined by CAP) and grossing techs must both meet High Complexity testing standards because CLIA does not differentiate between processing and grossing. Charles Embrey PA(ASCP) Histology Manager Carle Clinic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, April 18, 2007 9:55 AM To: Charles.Embrey; 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Charles, Now we are touching on another issue about grossing. Grossing is now different from processing. "Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized." If you have to exercise judgment on what to submit then it is "grossing" or "high complexity testing". If you are submitting all of the tissue then it is "processing". Tissue "processing" can be performed according to standardized protocols. Grossing techs have to follow CLIA and all of the requirements for high complexity testing. ANP.11610 Phase II N/A YES NO If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? This includes the 60 semester hours of sciences and other courses. If you are processing then you do not have to be "CLIA eligible". For years histo techs have done "processing" and when the CLIA thing came into place they were not eligible anymore. In my opinion this change (processing) was made because labs were having a difficult time finding the new CLIA grossing techs who meet the requirements. This was forcing labs to find anyone with a BS and training them to gross. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Wednesday, April 18, 2007 8:33 AM To: Douglas D Deltour; Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, Douglas has explained it better than anyone could. Keep in mind that if you are also CLIA certified you have to go by their stronger policy. Under CLIA '88 any grossing (even what CAP now calls "processing") is high complexity testing and the person performing it must qualify. It is a snare that is bound to catch someone. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, April 17, 2007 3:51 PM To: 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AWempren <@t> skcc.org Wed Apr 18 09:43:44 2007 From: AWempren <@t> skcc.org (Alexina Wempren) Date: Wed Apr 18 09:43:55 2007 Subject: [Histonet] CD34 Message-ID: What dilutions does anyone use for CD34? (for endothelial or pan-endothelial markers?) thx..........a wempren From rjbuesa <@t> yahoo.com Wed Apr 18 09:48:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 18 09:48:17 2007 Subject: [Histonet] difference between.. In-Reply-To: <46264748.A704.0070.0@uct.ac.za> Message-ID: <913171.93076.qm@web61218.mail.yahoo.com> Medical "technician" or laboratory "technician" is somebody usually with some level of licensure to complete less complex tasks in the laboratory (in our case the histology laboratory). The technician can for example embed, cut and routine stain, but should not do special stains or IHC, which are tasks that should be completed by the technologist. A laboratory assistant is somebody with the educational level described in your SOP (or in the hiring manual of your institution) that is usually a high school diploma, and that receives special training in the laboratory to do non-complex tasks, or those tasks that can be done automatically, like taking care of automated routine stainers or coverslippers, changing reagents in the tissue processor, recycling tasks and the like. Ren? J.` Michelle Perrins wrote: Hi What is the definition of a Laboratory Assistant and a Medical technician. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From JEllin <@t> yumaregional.org Wed Apr 18 09:49:10 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Apr 18 09:49:27 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <8B08EC394B366D4A895DD5686D6AFE4A58007D@LP-EXCHVS08.CO.IHC.COM> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8A6AF69@EXCHANGECLUSTER.yumaregional.local> Now I feel a flame coming on,, is this just a way to put higher responsibilites on Histotech and not receive additional pay?? Also is this type of "processing" addressed in the examination for a HT or HTL. I think that the excuse that there are not enough personnel does not warrant the fact that they are tyring to add additional duties on us without having to increase pay. To many times Histotech have had to increase their burden to help with shortages and additional testing duties. When are we going to draw the line and say "Why is CAP making decisions without first going through ASCP and making sure that the people doing the work are properly trained and are being tested for competencies!!"" This is why we are seeing the shortages, additional responsibilites without first providing way to succeed!!! Please do not give me the answer that the Pathologist over sees and is ultimaley responsible. A person working in the field is the one that is responsible for there actions, I can just see with a skin elipse that the person does not ink or proper orientate one time due to lack of knowledge or better yet they forget to describe the right color or texture of a specimen since they are only pouring things through a tea back or in a lens paper. We are not mindless robots here, and that is how I feel CAP continues to see us. I am a HISTO TECH and proud, I am also a PA, for that matter because of being a HT I have a better understanding on what quality needs to come from sectioning properly and describing things properly, WE ARE NOT MINDLESS ROBOTS!!! Any takers Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lance Erickson Sent: Wednesday, April 18, 2007 7:12 AM To: Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens It seems the CAP has once again completely messed up on the wording of checklist questions and left it up to vague interpretation as to what is meant by their wording. In an unfortunate use of the word "processing" the CAP has tried to make the "processing" of tissue into the gross description of small and uncomplicated tissue types. How they came to use this word is beyond common sense as the definition of tissue processing in any AP lab has been set since the onset of the tissue processing procedure when first used. This new CAP question is referring to their definition of "processing" in the notes section of ANP 11600. The set of questions 11600-11670 is about tissue grossing and not histology tissue processing. In an apparent attempt to disregard the standard set out by CLIA '88 for qualifications of the personnel performing tissue gross, they are making this "processing" "generally limited to small specimens (skin ellipses, small biopsies, curetting, etc.)and does not require knowledge of anatomy" into something that can be done by anyone in the lab. Actual grossing is defined by CAP as "tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. If you have anyone that is grossing in small and non-complex specimens then you need procedures for this. These people according to the CAP now do not have to qualify as "high complexity testing personnel" under CLIA-88. Lance Erickson Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From TJJ <@t> Stowers-Institute.org Wed Apr 18 09:49:41 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Apr 18 09:49:59 2007 Subject: [Histonet] A word of caution about posting private email responses Message-ID: Please Histonet users, If someone replies to you in a private manner rather than on list (public), do not forward this information to the list unless you have the permission of the sender first. Many have reasons to keep the response private, and probably specifically chose to answer directly rather than through the list. It is possible to copy/paste the information/text into an email in order to generate further discussion and information, and that is one way all could potentially benefit from the information while retaining the sender's anonymity. Personally speaking, I had an email response that I privately sent subsequently become a public post and I know it angered someone on the list. Additionally, others (JTT) have had difficulty when publicly offering strong opinions against certain products. In this case, if I were to email you privately and say "So-and-so's staining kit is crap and they should be horsewhipped for offering it" and you forwarded this to the list with commentary...I'm thinking that opens me up as a potential target. Please use discretion and think before you automatically turn a private response public. Carry on! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From rsrichmond <@t> aol.com Wed Apr 18 09:50:20 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Apr 18 09:50:35 2007 Subject: [Histonet] Re: Alcian Blue 8GX and S from someone who made it to sell In-Reply-To: <200704181013.1194626279611c@rly-mc04.mail.aol.com> References: <200704181013.1194626279611c@rly-mc04.mail.aol.com> Message-ID: <8C94FE005B7330C-154C-1D14@webmail-da06.sysops.aol.com> Just how you make Alcian blue has been unclear in the fifty or sixty years the dye's been on the market. It is, of course, an entirely synthetic product - it doesn't grow in little blue flowers. For many years Alcian blue was made in Germany, but the manufacturer decided that one of the synthetic intermediates (never publicly identified that I know of) was too hazardous to work with, and discontinued manufacture. A number of companies in China, India, and elsewhere began to make Alcian blue and sell it. None of them ever made clear how they made the dye. A few years ago Dick Dapson at ANATECH announced that he had invented an environmentally safe process for makiing Alcian blue, and ANATECH began to offer this product. They stated that they also sold it to other vendors - I don't know whether they ever made public who they sold it to. ANATECH's new synthetic process is also a trade secret. Manufacture in China and probably in other countries continues. I receive frequent e-mail advertisements for Alcian blue and other dyes from Suzhou Sinoera Chem Co. in China. (As a matter of fact, they've published an advertisement on Histonet today.) As far as I know, they have not publicly stated how they make Alcian blue. If I were purchasing Alcian blue and related dyes, I would be concerned that the product I bought was safe for the environment and for the workers who produce it. I have no connection with ANATECH, Suzhou Sinoera, or any other company involved in the manufacture or distribution of histologic dyes and related materials. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rjbuesa <@t> yahoo.com Wed Apr 18 09:51:55 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 18 09:52:01 2007 Subject: [Histonet] CD34 In-Reply-To: Message-ID: <335365.59617.qm@web61219.mail.yahoo.com> I used CD-34 from Becton Dickinson (mouse) at 1:100 with HIER at pH6 with placenta control. Ren? J. Alexina Wempren wrote: What dilutions does anyone use for CD34? (for endothelial or pan-endothelial markers?) thx..........a wempren _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From gcallis <@t> montana.edu Wed Apr 18 09:54:52 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Apr 18 09:54:42 2007 Subject: Dilution panel Re: [Histonet] CD34 In-Reply-To: References: Message-ID: <6.0.0.22.1.20070418084847.01b08740@gemini.msu.montana.edu> I recommend doing a dilution panel, starting at 10 to 20 ug/ml on a positive control tissue. This way you determine the correct antibody titer to fit your laboratory conditions. The company product data sheet should also give a recommended working concentration for their antibody, but a dilution panel is still a good idea. A dilution panel is something we do as a standard procedure for all primary antibodies in order to optimize the working concentration. This is important when one tries another source of the antibody as vendors may have a different clone than the one you have used before. At 08:43 AM 4/18/2007, you wrote: >What dilutions does anyone use for CD34? (for endothelial or > > pan-endothelial markers?) thx..........a wempren > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From doug <@t> ppspath.com Wed Apr 18 11:18:22 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Apr 18 10:18:09 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE4CA@EXCHANGEBE1.carle.com> Message-ID: What is CLIA's definition of "grossing tech" and "high complexity testing"? I have looked through the CLIA regs and have not seen a true definition. What a mess! :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 18, 2007 9:42 AM To: Douglas D Deltour; Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Douglas, You are right, according to CAP. You have to remember that CLIA and CAP are separate and not interconnected with their requirements. The problem is that CLIA has not changed the standard since CLIA '88 and it doesn't look like they are about to any time soon. My lab is both CAP and CLIA certified so I have to follow the stricter rule on grossing. CAP cannot dictate to CLIA or soften CLIA standards since they are two entirely different entities (civilian vs. government). As far as CLIA '88 dictates processing techs (as outlined by CAP) and grossing techs must both meet High Complexity testing standards because CLIA does not differentiate between processing and grossing. Charles Embrey PA(ASCP) Histology Manager Carle Clinic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, April 18, 2007 9:55 AM To: Charles.Embrey; 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Charles, Now we are touching on another issue about grossing. Grossing is now different from processing. "Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized." If you have to exercise judgment on what to submit then it is "grossing" or "high complexity testing". If you are submitting all of the tissue then it is "processing". Tissue "processing" can be performed according to standardized protocols. Grossing techs have to follow CLIA and all of the requirements for high complexity testing. ANP.11610 Phase II N/A YES NO If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? This includes the 60 semester hours of sciences and other courses. If you are processing then you do not have to be "CLIA eligible". For years histo techs have done "processing" and when the CLIA thing came into place they were not eligible anymore. In my opinion this change (processing) was made because labs were having a difficult time finding the new CLIA grossing techs who meet the requirements. This was forcing labs to find anyone with a BS and training them to gross. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Wednesday, April 18, 2007 8:33 AM To: Douglas D Deltour; Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, Douglas has explained it better than anyone could. Keep in mind that if you are also CLIA certified you have to go by their stronger policy. Under CLIA '88 any grossing (even what CAP now calls "processing") is high complexity testing and the person performing it must qualify. It is a snare that is bound to catch someone. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, April 17, 2007 3:51 PM To: 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Apr 18 10:37:46 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Apr 18 10:37:30 2007 Subject: [Histonet] Medical technician versus laboratory assistant Message-ID: <6.0.0.22.1.20070418090417.01b92e30@gemini.msu.montana.edu> I hope I can offer some insight on what a medical technician or MT(ASCP) but laboratory assistant is left as explanation by others, hopefully from someone who is a laboratory assistant. I would like to see that explanation myself. In other countries, the qualifications and even titles can be different - Canada has a different system for training and subsequent title(?). A medical technician,or MT(ASCP), in the USA will have a baccalaureate degree (complete four years of university) in some science curriculum which could be a BS in microbiology with a Med Tech option and an internship in an accredited laboratory/hospital setting. After the internship, they take a test for certification as an MT(ASCP). When I did my med tech training, 18 months internship was required before graduated from university - a long haul since it was 5 full years of college instead of 4 years. I later opted to take the HT(ASCP) histologic technician and HTL(ASCP) certification exams long after getting the MT(ASCP). MT's perform complex diagnostic testing in a laboratory including microbiology including parasitology, urinalysis, blood chemistry, hematology, serology, blood gases, virology, and probably more skills i.e. molecular biology related testing? In the 60's, med tech training still included histology with testing on our certification exam, but that has changed. The registry for histotechnicians (HT or HTL) is now a separate entity and certification, although potential MT's often have exposure to histotechnology during their training, and many MT's are also certified as HT and HTL's. I will let someone else explain an laboratory assistant. Also, the ASCP website should provide even more details on medical technicians. Hope this helps to some extent. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman Bozeman MT At 08:28 AM 4/18/2007, you wrote: >Hi >What is the definition of a Laboratory Assistant and a Medical >technician. >Michelle From Barry.R.Rittman <@t> uth.tmc.edu Wed Apr 18 10:48:49 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Apr 18 10:48:53 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8A6AF69@EXCHANGECLUSTER.yumaregional.local> Message-ID: Jesus Join the real world. This regression in jobs is not limited to the profession of histotechnology but pervades much of the business and academic community. Just look at Universities where faculty and staff have not increased significantly but individual work loads have increased sometimes dramatically (e.g. I now teach in 8 courses rather than 3). Unfortunately the bottom line seems to be driving this. When I was training I regarded loyalty as a two way street, both employer and employee benefited. This is no longer the case resulting in individuals in many jobs not regarding themselves as part of a team and therefore asking "what is in this job for me". While I do not personally support this attitude I can understand those who do. Our profession is unfortunately being more reactive than proactive. An example is the recent decision not to have a practical examination. I equate this to obtaining a driving license without a test of ability to drive. The only difference with our profession is that potential damage is often not as obvious. The age of robotic Histotechnology is some way in the future as far as we are concerned but just around the corner as far as most businesses are concerned. Let us hope that administrative synaptic firing will reach an appropriate level when there are too few histotechs to do the work needed. Joe that's twice you owe me. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Wednesday, April 18, 2007 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Now I feel a flame coming on,, is this just a way to put higher responsibilites on Histotech and not receive additional pay?? Also is this type of "processing" addressed in the examination for a HT or HTL. I think that the excuse that there are not enough personnel does not warrant the fact that they are tyring to add additional duties on us without having to increase pay. To many times Histotech have had to increase their burden to help with shortages and additional testing duties. When are we going to draw the line and say "Why is CAP making decisions without first going through ASCP and making sure that the people doing the work are properly trained and are being tested for competencies!!"" This is why we are seeing the shortages, additional responsibilites without first providing way to succeed!!! Please do not give me the answer that the Pathologist over sees and is ultimaley responsible. A person working in the field is the one that is responsible for there actions, I can just see with a skin elipse that the person does not ink or proper orientate one time due to lack of knowledge or better yet they forget to describe the right color or texture of a specimen since they are only pouring things through a tea back or in a lens paper. We are not mindless robots here, and that is how I feel CAP continues to see us. I am a HISTO TECH and proud, I am also a PA, for that matter because of being a HT I have a better understanding on what quality needs to come from sectioning properly and describing things properly, WE ARE NOT MINDLESS ROBOTS!!! Any takers Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lance Erickson Sent: Wednesday, April 18, 2007 7:12 AM To: Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens It seems the CAP has once again completely messed up on the wording of checklist questions and left it up to vague interpretation as to what is meant by their wording. In an unfortunate use of the word "processing" the CAP has tried to make the "processing" of tissue into the gross description of small and uncomplicated tissue types. How they came to use this word is beyond common sense as the definition of tissue processing in any AP lab has been set since the onset of the tissue processing procedure when first used. This new CAP question is referring to their definition of "processing" in the notes section of ANP 11600. The set of questions 11600-11670 is about tissue grossing and not histology tissue processing. In an apparent attempt to disregard the standard set out by CLIA '88 for qualifications of the personnel performing tissue gross, they are making this "processing" "generally limited to small specimens (skin ellipses, small biopsies, curetting, etc.)and does not require knowledge of anatomy" into something that can be done by anyone in the lab. Actual grossing is defined by CAP as "tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. If you have anyone that is grossing in small and non-complex specimens then you need procedures for this. These people according to the CAP now do not have to qualify as "high complexity testing personnel" under CLIA-88. Lance Erickson Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley.Chu <@t> moldev.com Wed Apr 18 11:41:39 2007 From: Shirley.Chu <@t> moldev.com (Shirley Chu) Date: Wed Apr 18 11:41:51 2007 Subject: [Histonet] Calif State Meeting Message-ID: <056AFE945F2C1F4292A1F8EBA9B84B5B049CA2BA@MI8NYCMAIL15.Mi8.com> The California Society for Histotechnology will hold is annual meeting on May 17-20 at the San Mateo Marriott located in the San Francisco Bay Area. Workshop Titles: Quality Control and Standardization of IHC Colors of Our Day Antibodies - 2007 The Cryostat and Technique Intro to Laser Capture Microdissection Technology Animal Tissue Stat-IHC Staining Fundamental IHC Utilizing Fine Needle Aspirations as an Aid in Tumor Diagnosis Interesting Things I Have Learned Through 40 years in Histology HPV Testing Methods Antibody Panels for the Clinical Laboratory A Family Affair: A Systems Approach to Team Development Quantitative Data Extraction: The Road Ahead for Histotechnology Basic Dynamics of Fixation and Processing Multi-Level Testing: A Working Algorithm for IHC Assay Development & Validation Ready or Not, Here It Comes: Microwave Technology Mini-Symposium: The Role of IHC in Tumor Diagnosis & Advances in Colorectal Cancer using MSI PCR Testing Managing a Winning Team - Communications is the Key The Chemistry of Fixaiton and Its Effect on Staining Please contact me if you wish an electronic copy of the full program. Shirley Chu President, California Society for Histotechnology Applications Scientist | Arcturus LCM Products Molecular Devices Corp 510-675-6260 | www.moleculardevices.com From tina.hammel <@t> usafa.af.mil Wed Apr 18 11:52:42 2007 From: tina.hammel <@t> usafa.af.mil (Hammel Tina M SSgt 10 MDSS/SGSC) Date: Wed Apr 18 11:52:47 2007 Subject: [Histonet] Jobs? Message-ID: <200704181652.l3IGqeuU006069@scorpion.usafa.af.mil> My husband and I will be moving to Delaware in June. If you have any histology job openings please let me know. My husband is certified and has been in histology for 5 years, and I have been in the military for 9 years as a histotech. Any information will be helpful. Tina Hammel From SecrestK <@t> wvuh.com Wed Apr 18 12:20:39 2007 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Wed Apr 18 12:22:11 2007 Subject: [Histonet] Cilia biopsies? Message-ID: <7708E0A8E46BDC40B23113F97FB0FB23031DCF5E@nt-exchange1.wvuh.wvuhs.com> Does anyone know of a reference laboratory that performs EM on cilia biopsies for motility? Thanks Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital From Charles.Embrey <@t> carle.com Wed Apr 18 12:33:50 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Apr 18 12:33:54 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <20070418152045.273D1203172@mx4.carle.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4CB@EXCHANGEBE1.carle.com> This is the CLIA '88 guideline for high complexity testing personnel- CLIA '88 493.1489 "On or before April 24 1995 (I) be a high school graduate or equivalent; and (b) have documentation of training appropriate for the test performed before analyzing patient specimens"...After that date it requires an associate degree in a biological or chemical science or medical laboratory technology -or- qualify as a medical technologist with a bachelor's degree from an accredited institution -or- earned a bachelor's degree in a chemical, physical, biologic or clinical laboratory science. A few years ago CLIA put out this interpretive guideline (printed below)to clear up the question of grossing. As you see it references 493.1498 (the above high complexity guideline". They basically sum up that if you examine/describe the tissue for record noting color weight or measurement then it is grossing and falls under 493.1498. Tissue processing as CAP call it involves noting size and other characteristics and qualifies as grossing under CLIA. The new federal register CLIA interpretive guidelines appendix C subpart M effective April 24, 2003 states in section 493.1461(e) "In the case if gross examinations, the technical supervisor may delegate to individuals qualified under 493.1498 the responsibility for the physical examination/description, including color, weight, measurement, and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed." Charles Embrey PA(ASCP) Histology Manager Carle Clinic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, April 18, 2007 11:18 AM To: Charles.Embrey; 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens What is CLIA's definition of "grossing tech" and "high complexity testing"? I have looked through the CLIA regs and have not seen a true definition. What a mess! :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 18, 2007 9:42 AM To: Douglas D Deltour; Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Douglas, You are right, according to CAP. You have to remember that CLIA and CAP are separate and not interconnected with their requirements. The problem is that CLIA has not changed the standard since CLIA '88 and it doesn't look like they are about to any time soon. My lab is both CAP and CLIA certified so I have to follow the stricter rule on grossing. CAP cannot dictate to CLIA or soften CLIA standards since they are two entirely different entities (civilian vs. government). As far as CLIA '88 dictates processing techs (as outlined by CAP) and grossing techs must both meet High Complexity testing standards because CLIA does not differentiate between processing and grossing. Charles Embrey PA(ASCP) Histology Manager Carle Clinic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, April 18, 2007 9:55 AM To: Charles.Embrey; 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Charles, Now we are touching on another issue about grossing. Grossing is now different from processing. "Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized." If you have to exercise judgment on what to submit then it is "grossing" or "high complexity testing". If you are submitting all of the tissue then it is "processing". Tissue "processing" can be performed according to standardized protocols. Grossing techs have to follow CLIA and all of the requirements for high complexity testing. ANP.11610 Phase II N/A YES NO If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? This includes the 60 semester hours of sciences and other courses. If you are processing then you do not have to be "CLIA eligible". For years histo techs have done "processing" and when the CLIA thing came into place they were not eligible anymore. In my opinion this change (processing) was made because labs were having a difficult time finding the new CLIA grossing techs who meet the requirements. This was forcing labs to find anyone with a BS and training them to gross. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Wednesday, April 18, 2007 8:33 AM To: Douglas D Deltour; Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, Douglas has explained it better than anyone could. Keep in mind that if you are also CLIA certified you have to go by their stronger policy. Under CLIA '88 any grossing (even what CAP now calls "processing") is high complexity testing and the person performing it must qualify. It is a snare that is bound to catch someone. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, April 17, 2007 3:51 PM To: 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Wed Apr 18 12:44:23 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Apr 18 12:44:48 2007 Subject: [Histonet] agar Message-ID: <462612A7020000AC00001860@nodcmsngwia1.trinity-health.org> Good Morning, Is there a recipe for this agar base ? Marcia From Charles.Embrey <@t> carle.com Wed Apr 18 12:45:32 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Apr 18 12:45:37 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8A6AF69@EXCHANGECLUSTER.yumaregional.local> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4CC@EXCHANGEBE1.carle.com> Jesus, You can't blame the doctors. At a time when budgets are getting tighter pathologists are understandably looking for the cheapest way to get the job done. There are too many histotechs that are happy in their job and don't want to leave that they will comply with the pathologists wishes. Everyone these days wants the most "bang for their buck" so until histotechs stand up and demand the extra money they deserve for taking on added responsibilities then it will continue to happen. JTT can attest to what I am saying. If they think you won't leave you are at their mercy. Hopefully most of us work for decent appreciative pathologists (like most of the ones I know) and don't have to worry about this but a few of us aren't that lucky. Like you I have an HT background and am certified HT so I do understand the situation from an HT perspective. I just don't know what will happen if ever CAP and CLIA should collide in court in a malpractice case. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Wednesday, April 18, 2007 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Now I feel a flame coming on,, is this just a way to put higher responsibilites on Histotech and not receive additional pay?? Also is this type of "processing" addressed in the examination for a HT or HTL. I think that the excuse that there are not enough personnel does not warrant the fact that they are tyring to add additional duties on us without having to increase pay. To many times Histotech have had to increase their burden to help with shortages and additional testing duties. When are we going to draw the line and say "Why is CAP making decisions without first going through ASCP and making sure that the people doing the work are properly trained and are being tested for competencies!!"" This is why we are seeing the shortages, additional responsibilites without first providing way to succeed!!! Please do not give me the answer that the Pathologist over sees and is ultimaley responsible. A person working in the field is the one that is responsible for there actions, I can just see with a skin elipse that the person does not ink or proper orientate one time due to lack of knowledge or better yet they forget to describe the right color or texture of a specimen since they are only pouring things through a tea back or in a lens paper. We are not mindless robots here, and that is how I feel CAP continues to see us. I am a HISTO TECH and proud, I am also a PA, for that matter because of being a HT I have a better understanding on what quality needs to come from sectioning properly and describing things properly, WE ARE NOT MINDLESS ROBOTS!!! Any takers Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lance Erickson Sent: Wednesday, April 18, 2007 7:12 AM To: Amy Self; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens It seems the CAP has once again completely messed up on the wording of checklist questions and left it up to vague interpretation as to what is meant by their wording. In an unfortunate use of the word "processing" the CAP has tried to make the "processing" of tissue into the gross description of small and uncomplicated tissue types. How they came to use this word is beyond common sense as the definition of tissue processing in any AP lab has been set since the onset of the tissue processing procedure when first used. This new CAP question is referring to their definition of "processing" in the notes section of ANP 11600. The set of questions 11600-11670 is about tissue grossing and not histology tissue processing. In an apparent attempt to disregard the standard set out by CLIA '88 for qualifications of the personnel performing tissue gross, they are making this "processing" "generally limited to small specimens (skin ellipses, small biopsies, curetting, etc.)and does not require knowledge of anatomy" into something that can be done by anyone in the lab. Actual grossing is defined by CAP as "tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. If you have anyone that is grossing in small and non-complex specimens then you need procedures for this. These people according to the CAP now do not have to qualify as "high complexity testing personnel" under CLIA-88. Lance Erickson Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marybeth.kaulahao <@t> thibodaux.com Wed Apr 18 12:49:50 2007 From: marybeth.kaulahao <@t> thibodaux.com (Marybeth Kaulahao) Date: Wed Apr 18 12:49:53 2007 Subject: [Histonet] GROSSING Message-ID: <003001c781e1$f67a1890$7496010a@trmc.thibodaux.com> Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth From Charles.Embrey <@t> carle.com Wed Apr 18 12:56:32 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Apr 18 12:56:36 2007 Subject: [Histonet] GROSSING In-Reply-To: <003001c781e1$f67a1890$7496010a@trmc.thibodaux.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4CD@EXCHANGEBE1.carle.com> I put my own lids on. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marybeth Kaulahao Sent: Wednesday, April 18, 2007 12:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Wed Apr 18 13:06:41 2007 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Wed Apr 18 13:06:59 2007 Subject: [Histonet] Expiration Dates on Reagents Message-ID: Hello all, Does anyone know of a list expiration dates for dry chemicals and reagents for histology? Thank you, Sally Norton Histology Children's Hospital and Regional Medical Center CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Wed Apr 18 13:08:25 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Apr 18 13:09:33 2007 Subject: [Histonet] GROSSING In-Reply-To: <003001c781e1$f67a1890$7496010a@trmc.thibodaux.com> Message-ID: <000901c781e4$8f937240$0d00a8c0@domain.Premier> This was along time ago when I was in clinical, but we also would put cassette lids on the specimens and place them in the fixative, decal, etc. for a few of the pathologists, not all of them, when they were grossing. It was not a big issue for us at the time since we had the gross room staffed with at least one person (a lab aide or a tech) most of the day to enter the specimens, print cassettes, handle frozen etc. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marybeth Kaulahao Sent: Wednesday, April 18, 2007 11:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From JSCHUMA1 <@t> Fairview.org Wed Apr 18 13:10:39 2007 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Wed Apr 18 13:10:16 2007 Subject: [Histonet] GROSSING In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE4CD@EXCHANGEBE1.carle.com> Message-ID: The pathologists, residents and pathology assistants here put their own lids on. Seems like a waste of time for two people in my humble opinion. Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 18, 2007 12:57 PM To: Marybeth Kaulahao; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GROSSING I put my own lids on. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marybeth Kaulahao Sent: Wednesday, April 18, 2007 12:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Wed Apr 18 13:23:03 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed Apr 18 13:23:07 2007 Subject: [Histonet] Expiration Dates on Reagents In-Reply-To: Message-ID: <138712.66585.qm@web55814.mail.re3.yahoo.com> Unless otherwise stated on the reagent label, we use 10yrs for dry chemicals and 5yrs for liquids (assuming proper storage). These dates are stated in our SOP. We do have the ability to extend the expiration date, especially for dry reagents that have a longer shelf life. Roger Moretz, Ph.D. Dept. of Toxicology Boehringer Ingelheim Pharmaceuticals Ridgefield, CT --- "Norton, Sally" wrote: > > > Hello all, > > > > Does anyone know of a list expiration > dates for dry > chemicals and reagents for histology? > > > > Thank you, > > > > Sally Norton > > Histology > > Children's Hospital and Regional Medical > Center > > > > > > > CONFIDENTIALITY NOTICE: This e-mail message, > including any attachments, is for the sole use of > the intended recipient(s) and may contain > confidential and privileged information protected by > law. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the > intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pruegg <@t> ihctech.net Wed Apr 18 13:26:10 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 18 13:26:08 2007 Subject: [Histonet] RE: [IHCRG] PDGF alpha, beta and receptors In-Reply-To: <6FF91AE4F1DC7743A6466E334EB865AE1A6E8E6B@VERITY.roswellpark.org> Message-ID: <008e01c781e7$0aeef750$6501a8c0@Patsy> Mary, I use PDGF B from Lab Vision RB-9257 at 1:100 steam AR for 25 min. with citrate buffer, hrp rab labeled polymer for 30 min., DAB or AEC (I do this on skin samples) for 10 min. I have not tried the others. Patsy _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Vaughan, Mary Sent: Monday, April 16, 2007 6:58 AM To: Histonet Subject: [IHCRG] PDGF alpha, beta and receptors good morning everyone- I have a proposed project that involves staining of PDGF alpha, beta, and the receptor for each. Does anyone have these protocols worked out? I'd appreciate some help, thanks. [PDGF-A, PDGFR-a, PDGF-B, PDGFR-b] Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From gu.lang <@t> gmx.at Wed Apr 18 13:39:07 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 18 13:38:41 2007 Subject: AW: [Histonet] GROSSING In-Reply-To: <003001c781e1$f67a1890$7496010a@trmc.thibodaux.com> Message-ID: <000801c781e8$d90333d0$6412a8c0@dielangs.at> Who labels the cassets, if the pathologist works alone? For us it is usual practice to assist at the grossing. We label the cassets, write a protocol (casset-list), give tissue-dyes, formalin-containers, HNO3 etc. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marybeth Kaulahao Gesendet: Mittwoch, 18. April 2007 19:50 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Wed Apr 18 15:06:58 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Apr 18 14:24:12 2007 Subject: [Histonet] GROSSING In-Reply-To: <003001c781e1$f67a1890$7496010a@trmc.thibodaux.com> Message-ID: It sounds like they are paying a technical salary for non-technical work. The only thing that can be done is do it or hire a histo-aid to do it. There is also the remote possibility that you can talk to the pathologist and voice your concerns. :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marybeth Kaulahao Sent: Wednesday, April 18, 2007 12:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Apr 18 14:27:06 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 18 14:27:20 2007 Subject: [Histonet] GROSSING In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE4CD@EXCHANGEBE1.carle.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70E1@EMAIL.archildrens.org> Our residents put their on lids on. But in my past I have worked for a place where we put the lids on the cassettes for all of our pathologists. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 18, 2007 12:57 PM To: Marybeth Kaulahao; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GROSSING I put my own lids on. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marybeth Kaulahao Sent: Wednesday, April 18, 2007 12:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From HornHV <@t> archildrens.org Wed Apr 18 14:40:05 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 18 14:40:20 2007 Subject: [Histonet] GROSSING In-Reply-To: <000801c781e8$d90333d0$6412a8c0@dielangs.at> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70E2@EMAIL.archildrens.org> We have the cassettes labeled and waiting for our residents to gross. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, April 18, 2007 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] GROSSING Who labels the cassets, if the pathologist works alone? For us it is usual practice to assist at the grossing. We label the cassets, write a protocol (casset-list), give tissue-dyes, formalin-containers, HNO3 etc. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marybeth Kaulahao Gesendet: Mittwoch, 18. April 2007 19:50 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] GROSSING Does anyone out there assist the pathologist during gross? We are in a bit of a fix over here. We did with our pathologist for years. She has retired. 2 guys took her place and one needs us to put the lids on the cassettes...because it makes gross go much faster for him. it is making the days when he does gross very long for us. The other pathologist doesn't need any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ day lab. my lab manager has asked me to ask other labs what they do. Anyone?? thanks Mary beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From tina.hammel <@t> usafa.af.mil Wed Apr 18 14:42:06 2007 From: tina.hammel <@t> usafa.af.mil (Hammel Tina M SSgt 10 MDSS/SGSC) Date: Wed Apr 18 14:42:10 2007 Subject: [Histonet] RE[HISTNET] GROSSING Message-ID: <200704181942.l3IJg7uS024601@scorpion.usafa.af.mil> I've only been in a military histo lab and for us we actually gross for the doctors and when they gross we write cassettes and put the lids on for them. My husband is a Civilian and in his lab the Docs gross the tissue in and put the tissue back into the containers then the histotechs have to make cassettes and take the tissue out of the containers and put into cassettes. From HornHV <@t> archildrens.org Wed Apr 18 14:42:53 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 18 14:43:18 2007 Subject: [Histonet] Cilia biopsies? In-Reply-To: <7708E0A8E46BDC40B23113F97FB0FB23031DCF5E@nt-exchange1.wvuh.wvuhs.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70E3@EMAIL.archildrens.org> ARUP Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Secrest, Kimberly Sent: Wednesday, April 18, 2007 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cilia biopsies? Does anyone know of a reference laboratory that performs EM on cilia biopsies for motility? Thanks Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From JBABBITT <@t> fresco.com Wed Apr 18 15:06:10 2007 From: JBABBITT <@t> fresco.com (Babbitt, Jeff) Date: Wed Apr 18 15:06:27 2007 Subject: [Histonet] Industrial Histology In-Reply-To: Message-ID: <2BA2AD943995314D9D98D68F33A0AC7B035B7766@exchange.fresco.local> Hello. As a relative newcomer to the field (and outside the medical and pathology fields to boot) I have some relatively basic questions concerning histology. My application is obtaining high-quality cross sections of multilayer film using a cryostat microtome, for optical and FTIR microscopic analysis. Does anyone have any experience with an application of this ilk? If so, I'd really like to compare notes. Thanks! RJ R. Jefferson Babbitt, Ph.D. Analytical Services Manager Fres-co System USA 3005 State Rd. Telford, PA 18969-1021 voice - 215-721-4600 x2149 cell - 267-236-4027 fax - 215-799-8017 email - jbabbitt@fresco.com From bsylinda <@t> aol.com Wed Apr 18 15:42:48 2007 From: bsylinda <@t> aol.com (bsylinda@aol.com) Date: Wed Apr 18 15:42:56 2007 Subject: [Histonet] (no subject) Message-ID: <8C9501142756159-1658-1ECA@webmail-stg-d01.sysops.aol.com> Hello Histoland, One of our pathologist has stated that some facilities have changed their way of reporting Her2 results from 1+, 2+, (grading system) etc. to just stating that the patient is simply positive or negative. If indeed you are doing this is there any literature that backs this way of reporting up. Thanks in advance, Sylinda Battle, HT (ASCP) ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From pmcardle <@t> ebsciences.com Wed Apr 18 14:55:21 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed Apr 18 15:59:26 2007 Subject: [Histonet] Old unwanted equipment Message-ID: <462677A9.2050706@ebsciences.com> Hello all: This e-mail is from a vendor BUT is entirely non-commercial; in fact, it is more "charitable" in nature: Equipment that most of us would consider "obsolete" is much needed in other areas of the world. Dr. Yaw Nsiah, a friend of mine and Professor at Eastern Connecticut State University, spends much of his spare time (and money) on a community health clinic in Ghana. Details may be found at http://www.ecsu.ctstateu.edu/personal/faculty/nsiahy/CRHPdoc2.htm I've offered to help spread the word in "my" circles of influence. There is more immediate need for certain equipment, much of which is outside the anatomic pathology domain (like old monitoring equipment), but centrifuges, microbiological equipment, etc. would also be much appreciated. Anyone with something they're looking to get rid of may contact me at EBS, or may contact Dr. Nsiah directly (nsiah@easternct.edu). Have a great day! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From pruegg <@t> ihctech.net Wed Apr 18 16:00:14 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 18 16:00:10 2007 Subject: [Histonet] Zinc Formalin and Iron stains In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0124755A@wahtntex2.waht.swest.nhs.uk> Message-ID: <00d001c781fc$9092a5e0$6501a8c0@Patsy> I have been using commercially made zinc formalin instead of formalin for the past 10+ years, I have not ever seen it make my tissues brittle or precipitate in any of the reagents on the tissue processor. As for retaining iron, there are other issues to consider besides just the fixative. Even alcohol dehydration can remove iron, (anything acidic), when I was in hematopathology we always did the iron stain on smears or GMA processed bone marrows without decal. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Tuesday, April 17, 2007 12:42 AM To: Liz Chlipala; Gayle Callis; Tarango, Mark; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Zinc Formalin and Iron stains Zinc also mordents haematoxylin resulting in a very intense nuclear stain; makes tissue brittle too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The bond that links your true family is not one of blood, but of respect and joy in each other's life. Rarely do members of one family grow up under the same roof. --Richard Bach This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 18 17:39:13 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 18 17:37:47 2007 Subject: [Histonet] GROSSING References: <5glv6h$1lic04f@orngca-mx-14.mgw.rr.com> Message-ID: <010b01c7820a$63f3e2c0$11614542@yourxhtr8hvc4p> In the hospital, there is a grossing tech who helps the pathologists like make cassettes, logs specimens in, makes bags for extra tissues and puts the cassettes in formalin. I love working with him because I get so spoiled. In the private lab, we make our own cassettes, bags, grossing logs and put our own cassettes in formalin. Some of the doctors do, ok, one doctor does, the other 3 need their hands held. Hey, they want to pay me a manager's salary to be a grossing assistant, I can live with that. JTT ----- Original Message ----- From: "Douglas D Deltour" To: "'Marybeth Kaulahao'" ; Sent: Wednesday, April 18, 2007 3:06 PM Subject: RE: [Histonet] GROSSING > It sounds like they are paying a technical salary for non-technical work. > The only thing that can be done is do it or hire a histo-aid to do it. > There > is also the remote possibility that you can talk to the pathologist and > voice your concerns. :) > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marybeth > Kaulahao > Sent: Wednesday, April 18, 2007 12:50 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] GROSSING > > Does anyone out there assist the pathologist during gross? We are in a bit > of a fix over here. We did with our pathologist for years. She has > retired. > 2 guys took her place and one needs us to put the lids on the > cassettes...because it makes gross go much faster for him. it is making > the > days when he does gross very long for us. The other pathologist doesn't > need > any one to stay with him. We are about a 7000 case /year 85-120 cassettes/ > day lab. my lab manager has asked me to ask other labs what they do. > Anyone?? > thanks > Mary beth > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 18 17:46:25 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 18 17:46:16 2007 Subject: [Histonet] Processing Specimens References: <44780C571F28624DBB446DE55C4D733A1FE4C9@EXCHANGEBE1.carle.com> Message-ID: <013b01c7820b$6643a9b0$11614542@yourxhtr8hvc4p> and people wonder why I have issues with CAP. Before this question was added, one of my pathologists (who is on the CAP board) told me that CAP was thinking that "Processing" was going to be consider any tissue that can be totally submitted, without inking or cutting, such as GI bxs cervical bxs, EMBs, etc. I told him that "processing" was going to get confused with processing like a tissue processor. He told me that CAP needed to distinguish between pouring tissue in a cassette and actually putting blade to tissue. Hey, I don't know what I'm talking about, now do I? ----- Original Message ----- From: "Charles.Embrey" To: "Douglas D Deltour" ; "Amy Self" ; Sent: Wednesday, April 18, 2007 8:32 AM Subject: RE: [Histonet] Processing Specimens Amy, Douglas has explained it better than anyone could. Keep in mind that if you are also CLIA certified you have to go by their stronger policy. Under CLIA '88 any grossing (even what CAP now calls "processing") is high complexity testing and the person performing it must qualify. It is a snare that is bound to catch someone. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, April 17, 2007 3:51 PM To: 'Amy Self'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing Specimens Amy, CAP defines processing as.... 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. So basically you need to have a "processing procedure" in place for the different type of specimens that are being "processed". If you have a PA or a Pathologist grossing everything then this would be N/A. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, April 17, 2007 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing Specimens Hello Histonetters, I was just thumbing through the checklist and came across a NEW question and wanted to see how some of you answered or would answer the following question; Thanks in advance, Amy ANP 11665 Are there written procedures for processing specimens. NOTE: this question applies if a non-pathologists process specimens. Amy Self Georgetown Memorial Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 18 17:48:34 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 18 17:48:21 2007 Subject: [Histonet] Expiration Dates on Reagents References: Message-ID: <016a01c7820b$b22f8ba0$11614542@yourxhtr8hvc4p> never seen one. We're still using the Picric Acid we had from the 1960's. I'm kidding, I'm kidding. JTT ----- Original Message ----- From: "Norton, Sally" To: Sent: Wednesday, April 18, 2007 1:06 PM Subject: [Histonet] Expiration Dates on Reagents Hello all, Does anyone know of a list expiration dates for dry chemicals and reagents for histology? Thank you, Sally Norton Histology Children's Hospital and Regional Medical Center CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 18 17:50:51 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 18 17:50:34 2007 Subject: [Histonet] difference between.. References: <86ADE4EB583CE64799A9924684A0FBBF0124758E@wahtntex2.waht.swest.nhs.uk> Message-ID: <017801c7820c$047f0480$11614542@yourxhtr8hvc4p> Kemlo, when you say naughty, how do you mean? I've been told that I'm very naughty. JTT ----- Original Message ----- From: "Kemlo Rogerson" To: "Michelle Perrins" ; Sent: Wednesday, April 18, 2007 9:37 AM Subject: RE: [Histonet] difference between.. What is the definition of a Laboratory Assistant and a Medical technician. Michelle In the UK a Laboratory Technician (now Biomedical Scientist) was state registered and if naughty could be thrown off; they could then come back as a Laboratory Assistant and be naughty again but this time supervised by a Biomedical Scientist who gets thrown off the register for letting the assistant be naughty. Dunno in America I guess you get sued. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The bond that links your true family is not one of blood, but of respect and joy in each other's life. Rarely do members of one family grow up under the same roof. --Richard Bach This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 18 17:53:34 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 18 17:53:15 2007 Subject: [Histonet] A word of caution about posting private email responses References: Message-ID: <018e01c7820c$654508f0$11614542@yourxhtr8hvc4p> Teri, I love it when you get physical (horse whipped) and talk dirty (staining kit is crap) oops, did I send this publicly again? JTT ----- Original Message ----- From: "Johnson, Teri" To: Sent: Wednesday, April 18, 2007 9:49 AM Subject: [Histonet] A word of caution about posting private email responses Please Histonet users, If someone replies to you in a private manner rather than on list (public), do not forward this information to the list unless you have the permission of the sender first. Many have reasons to keep the response private, and probably specifically chose to answer directly rather than through the list. It is possible to copy/paste the information/text into an email in order to generate further discussion and information, and that is one way all could potentially benefit from the information while retaining the sender's anonymity. Personally speaking, I had an email response that I privately sent subsequently become a public post and I know it angered someone on the list. Additionally, others (JTT) have had difficulty when publicly offering strong opinions against certain products. In this case, if I were to email you privately and say "So-and-so's staining kit is crap and they should be horsewhipped for offering it" and you forwarded this to the list with commentary...I'm thinking that opens me up as a potential target. Please use discretion and think before you automatically turn a private response public. Carry on! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Wed Apr 18 18:15:20 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Apr 18 18:15:41 2007 Subject: [Histonet] Expiration Dates on Reagents In-Reply-To: <016a01c7820b$b22f8ba0$11614542@yourxhtr8hvc4p> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6C80@NVCIEXCH02.NVCI.org> I worked in a lab about 10 years ago that had really old dry chemicals. The owner of that lab still used them and had several area hospital accounts. The oldest I remember seeing was 1916. I remember thinking, "wow that should be in a museum." She had the other high school kids that she hired dump formalin into the water reservoir that was next door. The lab has since closed. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, April 18, 2007 3:49 PM To: Norton, Sally; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Expiration Dates on Reagents never seen one. We're still using the Picric Acid we had from the 1960's. I'm kidding, I'm kidding. JTT ----- Original Message ----- From: "Norton, Sally" To: Sent: Wednesday, April 18, 2007 1:06 PM Subject: [Histonet] Expiration Dates on Reagents Hello all, Does anyone know of a list expiration dates for dry chemicals and reagents for histology? Thank you, Sally Norton Histology Children's Hospital and Regional Medical Center CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From JMacDonald <@t> mtsac.edu Wed Apr 18 19:56:36 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Apr 18 19:56:41 2007 Subject: [Histonet] Expiration Dates on Reagents In-Reply-To: Message-ID: When we have received dry chemicals without the expiration date, I called the company to ask. Hydroquinone is an example. The bottle was received new with no expiration date. I called the company and was told that once it was opened it was only stable for 1 year. Once reagents have been diluted the expiration date is shortened. The more dilute the reagent the shorter the expiration date for that reagent. Frieda Carson's book has a chart for expiration dates for prepared reagents, as does the staining manual of Charles Churukian. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Norton, Sally" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/18/2007 11:06 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Expiration Dates on Reagents Hello all, Does anyone know of a list expiration dates for dry chemicals and reagents for histology? Thank you, Sally Norton Histology Children's Hospital and Regional Medical Center CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Wed Apr 18 21:19:46 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Apr 18 21:19:52 2007 Subject: [Histonet] Picric Acid and teachers! Message-ID: <795391.33209.qm@web52505.mail.re2.yahoo.com> Hello all! I have been working for the Vet School for about nine months now and have learned many things. 1. When a cow is colored black it is in fact a bull. 2. On the Nile River Hippos eat crocodiles. 3. And deer have lights on their antlers, I am not sure if they have long lasting battery packs, or maybe extra long extension cords. Of course maybe a few even have solar panels. I have learned all of this from the person they hired to be my assistant. I am also teaching her histology, having a bit of trouble teaching the gram stain and the concept of until the blue runs off. We have not been discussing life changing events for the last few weeks. I am kind of exhausted pulling up facts on the net to show her the different breeds of animals and the male and female of each breed. I have also explained how the crocodile and hippo live in the same river, without the hippos becoming croc meat. (or crocs becoming hippo meat). And also that it is just a figure of speech to say "I know it was a buck by the way the light flashed off his antlers!" So what do our schools teach? Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Apr 19 01:39:02 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Apr 19 01:39:02 2007 Subject: [Histonet] difference between.. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247590@wahtntex2.waht.swest.nhs.uk> Kemlo, when you say naughty, how do you mean? I've been told that I'm very naughty Gulp, I hope you are, as Terry puts it, a girl!!! If you are then I'm pleased to hear it, pity about the pond! If you're not a girl, then thank god for the pond!!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The bond that links your true family is not one of blood, but of respect and joy in each other's life. Rarely do members of one family grow up under the same roof. --Richard Bach This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From raghulvet <@t> yahoo.co.in Thu Apr 19 04:32:22 2007 From: raghulvet <@t> yahoo.co.in (raghul) Date: Thu Apr 19 04:32:34 2007 Subject: [Histonet] alcian blue_ppt_thanks Message-ID: <704989.17584.qm@web8318.mail.in.yahoo.com> Dear members, thanks for the overwhelming response. I'll comeback soon after trying different options raghul Send a FREE SMS to your friend's mobile from Yahoo! Messenger. Get it now at http://in.messenger.yahoo.com/ From jlinda <@t> ces.clemson.edu Thu Apr 19 07:42:04 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Thu Apr 19 07:42:15 2007 Subject: [Histonet] RE:Industrial Histology Message-ID: <6.2.3.4.2.20070419082929.053f36f0@mailhost.ces.clemson.edu> Hi, RJ! You stated: "My application is obtaining high-quality cross sections of multilayer film using a cryostat microtome, for optical and FTIR microscopic analysis. Does anyone have any experience with an application of this ilk? If so, I'd really like to compare notes." 1. Well...been there, done that, NOT easy! Sounds like a perfect opportunity to use a tape-transfer technique. If you are not familiar with this technique, see http://www.instrumedics.com/cryojanetapetransferprocess.htm. 2. I would definitely use a high profile disposable blade. 3. Wear red the day you try sectioning! Now, there is no scientific evidence that this helps...it just seems to work for me:-) Good Luck! Linda (wearing red today) From michael.owen <@t> fda.hhs.gov Thu Apr 19 08:21:30 2007 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Thu Apr 19 08:21:44 2007 Subject: [Histonet] Expiration Dates on Reagents In-Reply-To: Message-ID: <15B0433665D1724BAE49F6E4476AD81C01F62807@FMD3VS012.fda.gov> Dear List Members, My laboratory had many reagents that were very old before it prepared for ISO 17025 status in 2004. I found a bottle of Methylene Blue powder dated from 1965 and a Loeffler's Alkaline Methylene Blue solution from 1987. :o) The facility has many individuals slow to change, therefore control of expired reagents can be a challenge occasionally. The laboratory is doing better than in the past. I will be interested to see what the A2LA re-assessment for ISO 17025 accreditation maintenance occurring next week will discover on this issue. :o) Based on observations like the ones given above, my laboratory has established the following guidelines for its work. Although it is a FDA facility, the concepts of CAPA and quality controls in their own laboratories is a new concept to many FDA scientists. * DISCLAIMER* I would not consider these SOPS/guidelines as ones that would be accepted in your own facilities if you are under FDA jurisdiction. Note also my laboratory primarly tests foods and performs limited histochemical work which is usually on pure cultures. :o) Quality Assurance of Microbiological Media, Reagents, and Locally Sterilized Equipment SOP 3. The following schedule will be followed for prepared media, components and reagents for which specific directions on expiration date are not provided: a. Tubed or bottled media (<1 liter): screw capped, 180 days; slip-capped, 90 days; some exceptions apply. Media in slip-capped tubes used less regularly may be stored at refrigeration temperature to avoid excessive evaporation. b. Large bottled media, including agar (> or = 1 liter): 90 days. c. Agar plates: 90 days (with some exceptions). d. Sterile reagents in screw capped container, e.g. saline, distilled water, mineral oil, and glycerol: 1 year. Chemical Mangaement SOP The Industrial Hygeinist will assign a five-year expiration date to stock chemcials started from the date of receipt. Five years will be the expiration date unless otherwise specified. For in-house prepared reagent solutions and/or solvent mixtures, an expiration date (shelf life span) of one year will be assigned unless another SOP or procedure dictates otherwise. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From elizabeth.heimrich <@t> bms.com Thu Apr 19 08:44:50 2007 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Apr 19 08:45:45 2007 Subject: [Histonet] Processing Specimens In-Reply-To: <013b01c7820b$6643a9b0$11614542@yourxhtr8hvc4p> References: <44780C571F28624DBB446DE55C4D733A1FE4C9@EXCHANGEBE1.carle.com> <013b01c7820b$6643a9b0$11614542@yourxhtr8hvc4p> Message-ID: <46277252.3090201@bms.com> To all concerned, What we have always called "putting a blade to tissue" is Trimming! How 'bout it. Why are they reinventing the wheel! Yes it is a 'Process', but Processing to anyone with histo background is definately not trimming or grossing. It is processing to paraffin. Why can't they just say submitting tissue, trimming tissue, or Wet process, should I go on? I feel sorry for all you HT, HTL certified persons that have to deal with the idiocy of bureaucracy!! Sometimes the simpliest answer is the best solution. I'm sorry.....just my 25 cents (inflationary 2 cents) Beth And on another note: paying someone manager wages to stand there and put cassette lids on....what a waste of knowledge/skill and money! And you are OK with IT? Like I said, I feel for ya!! No wonder there are less people entering the field of histology! If I had to stand there and put lids on..... I would probably quit!!!! Ther you have it my first histonet response!!! Joe Nocito wrote: > and people wonder why I have issues with CAP. Before this question was > added, one of my pathologists (who is on the CAP board) told me that > CAP was thinking that "Processing" was going to be consider any tissue > that can be totally submitted, without inking or cutting, such as GI > bxs cervical bxs, EMBs, etc. I told him that "processing" was going to > get confused with processing like a tissue processor. He told me that > CAP needed to distinguish between pouring tissue in a cassette and > actually putting blade to tissue. > Hey, I don't know what I'm talking about, now do I? > ----- Original Message ----- From: "Charles.Embrey" > > To: "Douglas D Deltour" ; "Amy Self" > ; > Sent: Wednesday, April 18, 2007 8:32 AM > Subject: RE: [Histonet] Processing Specimens > > > Amy, Douglas has explained it better than anyone could. Keep in mind > that if you are also CLIA certified you have to go by their stronger > policy. Under CLIA '88 any grossing (even what CAP now calls > "processing") is high complexity testing and the person performing it > must qualify. It is a snare that is bound to catch someone. > > Charles Embrey, PA(ASCP) > Histology Manager > Carle Clinic, IL > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas > D Deltour > Sent: Tuesday, April 17, 2007 3:51 PM > To: 'Amy Self'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Processing Specimens > > Amy, > > CAP defines processing as.... > 1) Processing is defined as a tissue examination limited to > description, > inking and cutting of the specimen (if applicable), and submission of > the > entire specimen to histology. Tissue processing can be performed > according > to standardized protocols. Processing is generally limited to small > specimens (skin ellipses, small biopsies, curettings, etc.) and does not > require knowledge of anatomy. > > So basically you need to have a "processing procedure" in place for the > different type of specimens that are being "processed". If you have a PA > or > a Pathologist grossing everything then this would be N/A. > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self > Sent: Tuesday, April 17, 2007 2:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing Specimens > > > > Hello Histonetters, > > I was just thumbing through the checklist and came across a NEW > question and wanted to see how some of you answered or would answer the > following question; Thanks in advance, Amy > > ANP 11665 > Are there written procedures for processing specimens. > NOTE: this question applies if a non-pathologists process > specimens. > > > > Amy Self > Georgetown Memorial Hospital > > > > NOTE: > The information contained in this message may be privileged, > confidential and protected from disclosure. > If the reader of this message is not the intended recipient, > or an employee or agent responsible for delivering this message > to the intended recipient, > you are hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. > If you have received this communication in error, > please notify us immediately by replying to this message and > deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.owen <@t> fda.hhs.gov Thu Apr 19 09:26:13 2007 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Thu Apr 19 09:26:24 2007 Subject: [Histonet] Expiration Dates on Reagents In-Reply-To: <15B0433665D1724BAE49F6E4476AD81C01F62807@FMD3VS012.fda.gov> Message-ID: <15B0433665D1724BAE49F6E4476AD81C01F62808@FMD3VS012.fda.gov> Dear List Members, Oops. I forgot one important item that probably does not relate to your work. :o) Chemical Management Peroxide-forming chemicals have an expiration date of six months after opening. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From denise.woodward <@t> uconn.edu Thu Apr 19 09:33:57 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Apr 19 09:34:05 2007 Subject: [Histonet] Oil Red O Controls In-Reply-To: <000a01c780a2$7d5a5dc0$afa2fea9@aa.uaeu.ac.ae> References: <804452.17014.qm@web61223.mail.yahoo.com> <000a01c780a2$7d5a5dc0$afa2fea9@aa.uaeu.ac.ae> Message-ID: A hot dog works terrific as an Oil Red O or Sudan Black B control. 'Course, you will never eat another one after you see the "tissue" it contains! Happy trails, Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Sciences Storrs, CT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evelyn Kaplan Sent: Monday, April 16, 2007 11:43 PM To: 'Rene J Buesa'; 'Greg Dobbin'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Oil Red O Controls I have used a piece of fixed fish liver for many years (not the same one, that is! :-)) and it makes an easy source of fat control tissue. Evelyn Kaplan, Dept. of Medical Education, Chair, BSc MLT Programme, Faculty of Medicine & Health Sciences, Al Ain, UAE. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 16 April 2007 16:49 To: Greg Dobbin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O Controls I used to cut FS of (+) materials and stored UNFIXED in a -80?C freezer. They were useful for several years. Ren? J. Greg Dobbin wrote: Hello All, I am interested in hearing what other labs are doing with regard to having good positive controls slides on hand. Are frozen sections cut from normal breast tissue and stored? How? Fixed or unfixed? Room temp or freezer? Thanks in advance! -- Greg Dobbin Chief Technologist Histology Queen Elizabeth Hospital Charlottetown, PE Canada Ph (902) 894-2337 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angela.mcnabola <@t> ikonisys.com Thu Apr 19 09:59:16 2007 From: angela.mcnabola <@t> ikonisys.com (Angela McNabola) Date: Thu Apr 19 09:59:25 2007 Subject: [Histonet] Cyto people out there? Message-ID: <4ECD18F12E443644B1F3C924A203982417DCCC@ikoexchange.ikonisys.com> Hi all, Question for anyone who is doing/ has done/ shares a lab with someone who does FISH on cells (nuclei) from suspensions, smears, etc. Would you be willing to share how you pretreat your slides before staining? I'm interested in your basic home-made protocols (pepsin/HCL) as well as any commercially available ones that have worked for you or any other methods. Thanks in advance! Angela Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com From Karen.Heckford <@t> CHW.edu Thu Apr 19 10:23:36 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Apr 19 10:23:42 2007 Subject: [Histonet] Peanut Oil/Oliver Oil Message-ID: Good Morning, I have been asked to do a Fite stain this morning. I have not done one in years so of course I do not have any peanut oil on hand. I just thought possibly I could use olive oil since I can get it down in the Cafeteria. Has anyone ever tried this? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From stamptrain <@t> yahoo.com Thu Apr 19 10:34:21 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Apr 19 10:34:28 2007 Subject: [Histonet] Peanut Oil/Oliver Oil In-Reply-To: Message-ID: <200356.75410.qm@web55806.mail.re3.yahoo.com> Sorry, can't help myself. I don't know about Oliver Oil but maybe Popeye can help with Olive Oyl. Roger Moretz --- "Heckford, Karen - SMMC-SF" wrote: > Good Morning, I have been asked to do a Fite stain > this morning. I have > not done one in years so of course I do not have any > peanut oil on hand. I > just thought possibly I could use olive oil since I > can get it down in the > Cafeteria. Has anyone ever tried this? > Cheers, > > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Apr 19 10:38:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 19 10:38:11 2007 Subject: [Histonet] Peanut Oil/Oliver Oil In-Reply-To: Message-ID: <347892.35662.qm@web61213.mail.yahoo.com> Under the same circumstances, I once did and it came well. Ren? J. "Heckford, Karen - SMMC-SF" wrote: Good Morning, I have been asked to do a Fite stain this morning. I have not done one in years so of course I do not have any peanut oil on hand. I just thought possibly I could use olive oil since I can get it down in the Cafeteria. Has anyone ever tried this? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From Karen.Heckford <@t> CHW.edu Thu Apr 19 12:00:58 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Apr 19 12:01:13 2007 Subject: FW: [Histonet] Peanut Oil/Oliver Oil Message-ID: -----Original Message----- From: Heckford, Karen - SMMC-SF Sent: Thursday, April 19, 2007 10:00 AM To: 'Roger Moretz' Subject: RE: [Histonet] Peanut Oil/Oliver Oil Sorry everyone about the Oliver Oil. What can you expect it is Thursday and I have been up since 3am. So much for poof reading. Oops Proof Reading. AUGH!!!!! One more day until the weekend. I actually wrote Oliver Oil twice and did not catch the Subject one. -----Original Message----- From: Roger Moretz [mailto:stamptrain@yahoo.com] Sent: Thursday, April 19, 2007 8:34 AM To: Heckford, Karen - SMMC-SF; Histonet (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Peanut Oil/Oliver Oil Sorry, can't help myself. I don't know about Oliver Oil but maybe Popeye can help with Olive Oyl. Roger Moretz --- "Heckford, Karen - SMMC-SF" wrote: > Good Morning, I have been asked to do a Fite stain this morning. I > have not done one in years so of course I do not have any peanut oil > on hand. I just thought possibly I could use olive oil since I can > get it down in the Cafeteria. Has anyone ever tried this? > Cheers, > > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From relia1 <@t> earthlink.net Thu Apr 19 12:18:59 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Apr 19 12:19:05 2007 Subject: [Histonet] Cyto help. Can you help? (Slightly off-topic) Message-ID: <7796584.1177003139594.JavaMail.root@elwamui-chisos.atl.sa.earthlink.net> Hi Histonetters, A posting I saw this morning looking for cyto help gave me an idea. I also need some help. I am currently working on a recruiting assignment for one of my histology clients to assist them in finding a cytology supervisor. As you know I specialize in permanent placement in histology. The help I need is if you know any cytology supervisors that might be interested in a new opportunity could you please pass this e-mail on to them? This is a full time, day shift permanent position located in the Pittsburgh area. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Again I realize this is off topic and if anyone is offended by this posting I would like to apologize in advance. I really appreciate you taking the time to read this post. Thanks Again - Pam Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1@earthlink.net Toll Free: 866-60-RELIA (866-607-3542) From soper <@t> ciwemb.edu Thu Apr 19 12:27:50 2007 From: soper <@t> ciwemb.edu (Sarah Clatterbuck Soper) Date: Thu Apr 19 12:28:24 2007 Subject: [Histonet] problem with "foggy" DAPI staining Message-ID: <4627A696.9010802@ciwemb.edu> Hi all, First I want to thank you all for being such a great resource. I learn so much from this list! But now I have a question of my own. I've been having a problem for a while now, and I haven't been able to figure out what is going on. I hope you can supply some ideas. :-) I'm a graduate student working with mouse testes. My usual procedure is to dissect out the testes, fix overnight in Bouin's, process over the next night (1 hour per station), embed, and section at 6 microns. I then PAS-H stain or perform fluorescence immunostaining. If I am immunostaining I always counterstain with DAPI. This procedure works perfectly for wild-type adult testes. For juvenile mice or for our mutant mice, however, my DAPI staining looks terrible. It is fuzzy and diffuse looking, as if it is out of focus. It's like looking at the DAPI though a fog. If I PAS-H stain these sections they look fine. Immunostaining still works. It's just the DNA staining that looks really crummy. I've had wild-type and mutant testes processed side by side all the way through; the wild-type looks great and the mutants look foggy. Obviously, the difference must have to do with the size of the tissues--the mutant testes weigh 25-35 mg, or about 1/4-1/3 of the wild-types. So I'm guessing I'm over-fixing or over-processing or something. I tend not to think it is over-fixing, as I've forgotten some WT testes in Bouin's and processed them weeks later, and they still look ok. So my guess is over-processing, though is seems sort of bizarre to me that processing would screw up the DNA somehow. Does this seem likely to you? Do you have any other ideas? Thanks so much for any help you can offer! Sarah From Sarah.Jones <@t> dako.com Thu Apr 19 12:53:01 2007 From: Sarah.Jones <@t> dako.com (Sarah Jones) Date: Thu Apr 19 12:51:53 2007 Subject: [Histonet] Top ten stains Message-ID: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM From Sarah.Jones <@t> dako.com Thu Apr 19 13:14:11 2007 From: Sarah.Jones <@t> dako.com (Sarah Jones) Date: Thu Apr 19 13:13:01 2007 Subject: [Histonet] For Shirley Chu Message-ID: <8B07D141BCDE434285DC12B3290E3FB3010239CC@exbackca.caus.dako.net> I would appreciate it if you could send me an electronic form of the CSH May meeting. Thank you!! Sarah A. Jones, HTL (ASCP) CM hawkmoon1515@yahoo.com From Barry.R.Rittman <@t> uth.tmc.edu Thu Apr 19 14:19:05 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Apr 19 14:19:10 2007 Subject: [Histonet] Peanut Oil/Oliver Oil In-Reply-To: Message-ID: Karen I am sure that Popeye will not mind a bit. B -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, April 19, 2007 12:01 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: FW: [Histonet] Peanut Oil/Oliver Oil -----Original Message----- From: Heckford, Karen - SMMC-SF Sent: Thursday, April 19, 2007 10:00 AM To: 'Roger Moretz' Subject: RE: [Histonet] Peanut Oil/Oliver Oil Sorry everyone about the Oliver Oil. What can you expect it is Thursday and I have been up since 3am. So much for poof reading. Oops Proof Reading. AUGH!!!!! One more day until the weekend. I actually wrote Oliver Oil twice and did not catch the Subject one. -----Original Message----- From: Roger Moretz [mailto:stamptrain@yahoo.com] Sent: Thursday, April 19, 2007 8:34 AM To: Heckford, Karen - SMMC-SF; Histonet (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Peanut Oil/Oliver Oil Sorry, can't help myself. I don't know about Oliver Oil but maybe Popeye can help with Olive Oyl. Roger Moretz --- "Heckford, Karen - SMMC-SF" wrote: > Good Morning, I have been asked to do a Fite stain this morning. I > have not done one in years so of course I do not have any peanut oil > on hand. I just thought possibly I could use olive oil since I can > get it down in the Cafeteria. Has anyone ever tried this? > Cheers, > > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 19 14:23:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 19 14:23:05 2007 Subject: [Histonet] Top ten stains In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> Message-ID: <784854.33274.qm@web61218.mail.yahoo.com> My "top ten" used to be: 1-PAS with / without diastase 2-Modified Steiner 3-Giemsa 4-Reticulin (silver) 5-Methenamine silver 6-ACID fast 7-Brown-Brenn 8-Trichrome (masson) 9-van Gieson 10-Mucicarmine These procedures will be determined by the type of lab and for sure will be different between labs Ren? J. Sarah Jones wrote: I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From elizabeth.heimrich <@t> bms.com Thu Apr 19 14:51:40 2007 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Apr 19 14:51:48 2007 Subject: [Histonet] testes fixation/ staining Message-ID: <4627C84C.9070501@bms.com> The only thing I can say is that Picric acid left in tissue ultimately affects the staining of the nuclei if not neutralized with EtOH saturated w/ Li2CO3. There is a much better fixative of testes 'Modified Davidson's Fluid'. The pathologist I worked with, Dr Diane Creasy, published her findings in TOXICOLOGIC PATHOLOGY vol. 30, no 4, pp524-533, 2002, entitled _Fixation of Testes and Eyes Using a Modified Davidson's Fluid: Comparison with Bouin's Fluid and Conventional Davidson's Fluid._ This article describes the recipe for mDavidson's and compares it to other Fixatives routinely used. Best of Luck! Beth From Laurie <@t> conxis.com Thu Apr 19 18:29:14 2007 From: Laurie <@t> conxis.com (Laurie Popp) Date: Thu Apr 19 18:31:02 2007 Subject: [Histonet] RE: ZN stain (Liz Chlipala) Message-ID: <003c01c782da$8b794350$9700a8c0@laurie> Hi Liz, If I remember correctly when we were studying the chapter on acid fast microbes a couple of weeks ago there was a notation in Carson's book about the outer cell wall of m. Tb becoming non-acid fast if exposed to alcohols thus yielding a false negative. The alcohol reacts with the lipid in the membrane and strips it away if I remember correctly. Laurie Popp HT Candidate Mayo Clinic, Rochester From lpaveli1 <@t> hurleymc.com Thu Apr 19 19:12:59 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Apr 19 19:13:29 2007 Subject: [Histonet] Top ten stains Message-ID: <4627CD4C020000EE00013576@smtp-gw.hurleymc.com> Sarah, Seems that more and more of our stains are turning towards IHC, but these are the most frequently used in our 461 bed hospital. 1. Diff Quik Giemsa for H.P. 2. Perl's Iron 3. Pas (w & w/o diastase) 4. Gomori's Trichrome 5. Reticulum 6. GMS 7. AFB 8. FAFB 9. Mucicarmine 10. Brown & Brenn's gram stain Lynette >>> "Sarah Jones" 04/19/07 1:53 PM >>> I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From conniegrubaugh <@t> hotmail.com Thu Apr 19 19:14:03 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Thu Apr 19 19:14:06 2007 Subject: [Histonet] Charlie Brown Message-ID: I'm looking for Charlie Brown. Please contact me. Connie G. _________________________________________________________________ Connect to the next generation of MSN Messenger? http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From scorpionrider <@t> cox.net Thu Apr 19 19:58:57 2007 From: scorpionrider <@t> cox.net (Mark Turner) Date: Thu Apr 19 19:59:04 2007 Subject: [Histonet] Top ten stains In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> Message-ID: <000001c782e7$132756f0$6400a8c0@D8P565C1> Sarah, Just off the top of my head, without any documentation supporting it (I know, a truly deficient way to operate, lol), I find our top 10 special stains to be: 1. PAS 2. PASD 3. Masson's Trichrome 4. GMS 5. Periodic Acid - Methenamine Silver (PAMS) 6. Iron 7. Congo Red 8. AFB 9. Elastic Van Gieson 10. Alcian Blue / PAS Let us know what you compile as the "Top Ten" Mark -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Jones Sent: Thursday, April 19, 2007 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Top ten stains I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Apr 19 20:15:22 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 19 20:15:26 2007 Subject: [Histonet] Charlie Brown In-Reply-To: Message-ID: <940682.58029.qm@web31308.mail.mud.yahoo.com> Which Ckarlie Brown? The one at NIH or the one that was at Quest LV, Nevada, Mercy, Sacramento, CA and now at UC Davis, CA? I have both contacts in my personal info files. Akemi Allison-Tacha --- connie grubaugh wrote: > I'm looking for Charlie Brown. Please contact me. > Connie G. > _________________________________________________________________ > Connect to the next generation of MSN Messenger? > http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Apr 19 20:42:26 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 19 20:42:30 2007 Subject: [Histonet] Which Charlie Brown? Message-ID: <134489.52178.qm@web31313.mail.mud.yahoo.com> Charlie from NIH & UC Davis would of course have to give me permission to divulge their contact info. Akemi From BMolinari <@t> heart.thi.tmc.edu Fri Apr 20 05:53:28 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Apr 20 05:53:33 2007 Subject: [Histonet] Top ten stains In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> Message-ID: Hi Sarah, 1) Masson Trichrome 2) Verhoff's Van Gieson 3 Picro-Sirius Red 4) Movats 5) Brown and Brenn 6) Von Kossa 7) PTAH 8) Toluidine Blue 9) PAS 10) Carstairs Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Jones Sent: Thursday, April 19, 2007 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Top ten stains I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 20 06:36:48 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Apr 20 06:36:54 2007 Subject: [Histonet] Charlie Brown Message-ID: <407F05A128805F4C879A33DBA32E618E20C2CA@TRFT-EX01.xRothGen.nhs.uk> Is this a continuation of the peanuts theme? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: 20 April 2007 01:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charlie Brown I'm looking for Charlie Brown. Please contact me. Connie G. _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Apr 20 06:39:49 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Apr 20 06:39:54 2007 Subject: [Histonet] Charlie Brown References: <407F05A128805F4C879A33DBA32E618E20C2CA@TRFT-EX01.xRothGen.nhs.uk> Message-ID: If so I bet the little red-haired girl knows where he is. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Friday, April 20, 2007 7:37 AM To: connie grubaugh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Charlie Brown Is this a continuation of the peanuts theme? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: 20 April 2007 01:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charlie Brown I'm looking for Charlie Brown. Please contact me. Connie G. _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Temple <@t> ssfhs.org Fri Apr 20 06:52:12 2007 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Fri Apr 20 06:52:52 2007 Subject: [Histonet] Top ten stains In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> Message-ID: AFB GMS PAS/Light Green for Fungus Masson Trichrome Alcian Blue pH 2.5 Colloidal Iron PAS with and w/o diastase Brown & Brenn Congo Red Retic Nancy Temple St. Francis Hospital Indianapolis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Jones Sent: Thursday, April 19, 2007 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Top ten stains I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and destroy all copies of the original message. Thank you. From TMcNemar <@t> lmhealth.org Fri Apr 20 06:57:36 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Apr 20 06:57:50 2007 Subject: [Histonet] Top ten stains In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F483@lmhsmail.lmhealth.org> Here's ours: 1. Alcian Blue - PAS 2. Iron 3. PAS 4. Reticulin 5. Masson's Trichrome 6 Mucicarmine 7. PAS (fungal) 8. GMS 9. Acid Fast 10. Verhoeff's Elastic Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sarah Jones Sent: Thursday, April 19, 2007 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Top ten stains I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Apr 20 07:02:28 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Apr 20 07:02:32 2007 Subject: [Histonet] hot air oven for drying slides temp Message-ID: I was under the impression that the temp of the drying oven in a stainer or a hot air oven if stained seperately should be aroung 60 degrees--just above the melting point of the paraffin. I have recently been in a lab where they dry their routine and special stain slides at 95 degrees. The leave their immunos at room temp over night. Their seems to be no complaints by the pathologists with the temp that high but I was wondering what the averge temp others are using. Thanks Diana From rjbuesa <@t> yahoo.com Fri Apr 20 07:18:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 20 07:18:42 2007 Subject: [Histonet] hot air oven for drying slides temp In-Reply-To: Message-ID: <391593.85669.qm@web61217.mail.yahoo.com> Diana: And are you surprised? Remember that histology is not even close to a science, it is an art and that is why each lab does things pretty much as they want to, as long as "things are OK", until one day when things are not OK any longer and they turn to Histonet for answers. In my personal experience: you do not need to leave slides for IHC at room temp. overnight; they will require melting the paraffin anyway and it is unnecessary the overnight step. Also you are right about "just above" the MP of the parafin; around 60?C and the pathologists do not complain because they ususally do not have any idea on how histology is done, until one day also when they start complaining and asking WHY?. Heating sections to 95?C is not only unnecessary but will affect some staining, like nuclear with hematoxylin. If you heat all your slides to just 60?C, including those for IHC, keep doing that and do not pay attentions to others. Practice histology as usual, doing what is OK for you (untill one improbable day when there will be working standards for all labs, NOT). Ren? J. Diana McCaig wrote: I was under the impression that the temp of the drying oven in a stainer or a hot air oven if stained seperately should be aroung 60 degrees--just above the melting point of the paraffin. I have recently been in a lab where they dry their routine and special stain slides at 95 degrees. The leave their immunos at room temp over night. Their seems to be no complaints by the pathologists with the temp that high but I was wondering what the averge temp others are using. Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From pruegg <@t> ihctech.net Fri Apr 20 07:37:29 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Fri Apr 20 07:36:40 2007 Subject: [Histonet] hot air oven for drying slides temp In-Reply-To: Message-ID: <200704201236.l3KCaWNg024973@pro12.abac.com> There was an article in JOH last year I believe on the effects of slide drying temps on IHC, it was very interesting and yes high temps can affect some antigens. I keep my drying oven at 60dc or below. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, April 20, 2007 6:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hot air oven for drying slides temp I was under the impression that the temp of the drying oven in a stainer or a hot air oven if stained seperately should be aroung 60 degrees--just above the melting point of the paraffin. I have recently been in a lab where they dry their routine and special stain slides at 95 degrees. The leave their immunos at room temp over night. Their seems to be no complaints by the pathologists with the temp that high but I was wondering what the averge temp others are using. Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Apr 20 07:43:59 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Apr 20 07:45:11 2007 Subject: [Histonet] Top ten stains In-Reply-To: <4627CD4C020000EE00013576@smtp-gw.hurleymc.com> Message-ID: <000501c78349$944660c0$d00f7ca5@lurie.northwestern.edu> Sarah, I can't quite come up with 10 as we are a research lab and do more IHC than anything else,but here goes: 1. Masson Trichrome 2. Safranin O 3. Iron 4. PAS 5. Movat's Pentachrome 6. May-Grunwald Giemsa 7. Retic 8. Sirius Red 9. GMS 10. Toluidine Blue That should be interesting in your tally. IHC would be ER/PR, Her2, Ki67. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center Chicago,IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, April 19, 2007 6:13 PM To: Sarah.Jones@dako.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Top ten stains Sarah, Seems that more and more of our stains are turning towards IHC, but these are the most frequently used in our 461 bed hospital. 1. Diff Quik Giemsa for H.P. 2. Perl's Iron 3. Pas (w & w/o diastase) 4. Gomori's Trichrome 5. Reticulum 6. GMS 7. AFB 8. FAFB 9. Mucicarmine 10. Brown & Brenn's gram stain Lynette >>> "Sarah Jones" 04/19/07 1:53 PM >>> I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Apr 20 07:54:55 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Apr 20 07:58:57 2007 Subject: [Histonet] hot air oven for drying slides temp References: <200704201236.l3KCaWNg024973@pro12.abac.com> Message-ID: For those interested it is Volume 28, Number 1, March 2005. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy ruegg Sent: Friday, April 20, 2007 8:37 AM To: 'Diana McCaig'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] hot air oven for drying slides temp There was an article in JOH last year I believe on the effects of slide drying temps on IHC, it was very interesting and yes high temps can affect some antigens. I keep my drying oven at 60dc or below. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, April 20, 2007 6:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hot air oven for drying slides temp I was under the impression that the temp of the drying oven in a stainer or a hot air oven if stained seperately should be aroung 60 degrees--just above the melting point of the paraffin. I have recently been in a lab where they dry their routine and special stain slides at 95 degrees. The leave their immunos at room temp over night. Their seems to be no complaints by the pathologists with the temp that high but I was wondering what the averge temp others are using. Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TownsendD <@t> childrensdayton.org Fri Apr 20 08:07:11 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Apr 20 08:07:40 2007 Subject: [Histonet] hot air oven for drying slides temp Message-ID: Our temperature range is from 58 to 70 degrees. We usually keep it around 65-68 degrees. Dolores From timothy.macatee <@t> med.nyu.edu Fri Apr 20 08:13:07 2007 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Apr 20 08:15:28 2007 Subject: [Histonet] Top ten stains In-Reply-To: Message-ID: 1. Ketchup 2. Nacho cheese 3. Paraffin (of course) 4. Gravy 5. Mustard 6. pasta sauce 7. diet coke 8. coffee 9. butter 10. eosin Oh, you mean for slides. It is Friday. -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From pmcardle <@t> ebsciences.com Fri Apr 20 08:25:49 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Apr 20 08:25:55 2007 Subject: [Histonet] Re: paraffin heating Message-ID: <4628BF5D.2090502@ebsciences.com> Hi: Glad they're working out. First, they are single use and second, ALWAYS silver side up. Please don't hesitate to contact me with any questions or issues you may have. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com -------- Original Message -------- Subject: paraffin heating Date: Fri, 20 Apr 2007 09:18:03 -0400 From: Mike Scott To: pmcardle@ebsciences.com Dear Mr. McCardle: Thanks for samples of the microwave sheets. The paraffin heats quick and the artifacts are gone, otherwise no difference (good). We're going to order some, but someone told me I can flip them over and use them twice, is this correct? Mike Scott From POWELL_SA <@t> Mercer.edu Fri Apr 20 08:54:21 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Apr 20 08:55:30 2007 Subject: [Histonet] Charlie Brown In-Reply-To: Message-ID: <01MFN2LAAKPS8X1GQX@Macon2.Mercer.edu> Oh no, get Snoopy to find him. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, April 20, 2007 7:40 AM To: Marshall Terry Dr, Consultant Histopathologist; connie grubaugh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Charlie Brown If so I bet the little red-haired girl knows where he is. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Friday, April 20, 2007 7:37 AM To: connie grubaugh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Charlie Brown Is this a continuation of the peanuts theme? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: 20 April 2007 01:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charlie Brown I'm looking for Charlie Brown. Please contact me. Connie G. _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Apr 20 08:56:58 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Apr 20 08:57:24 2007 Subject: [Histonet] Charlie Brown Message-ID: I saw him with Snoopy down at the corner store, buy some ice cream....happy Friday!!! Robyn >>> "connie grubaugh" 4/19/2007 5:14 PM >>> I'm looking for Charlie Brown. Please contact me. Connie G. _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Apr 20 09:13:42 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Apr 20 09:13:48 2007 Subject: [Histonet] Happy Friday and National Medical Lab Professionals Appreciation Week Message-ID: <08A0A863637F1349BBFD83A96B27A50A12002B@uwhis-xchng3.uwhis.hosp.wisc.edu> OK, with it being Friday and all. I thought everyone needed a bit of a celebration on making it through another week without killing any pathologists/bosses (apologies to you pathologists out there). Here is a quote I found somewhere. "Ah the Professional Histologist! Walk in awe when you meet one...Because there are only few people who can produce something as beautiful and useful as a slide." Drs William Osler and F.E. Mohs So smile, put a spring in your step, and at least acknowledge you co-workers for the good job they do. If we expect others to respect the work we do, we must start with ourselves. (opps, waxing philosophical, sorry) It might also be a good idea to post this quote "inconspicuously" around the lab next week. After all, if the great Dr. F.E. Mohs recognized our value, maybe the other docs will consider the idea. :) HAPPY NATIONAL MEDICAL LAB PROFESSIONAL APPRECIATION WEEK!!! Claire Ingles UW Hospital West Clinic Mohs Lab Madison WI From alaskagirl1950 <@t> yahoo.com Fri Apr 20 09:26:08 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Apr 20 09:26:15 2007 Subject: Fwd: [Histonet] Happy Friday and National Medical Lab Professionals Appreciation Week Message-ID: <756710.63823.qm@web52502.mail.re2.yahoo.com> This was way cool! Thank you for these words, been having a confusing week, trying to teach simple things. Starting to feel very unimportant. And I love Histology so much! --- Ingles Claire wrote: > Date: Fri, 20 Apr 2007 09:13:42 -0500 > From: "Ingles Claire" > To: > Subject: [Histonet] Happy Friday and National > Medical Lab Professionals > Appreciation Week > > OK, with it being Friday and all. I thought > everyone needed a bit of a celebration on > making it through another week without killing > any pathologists/bosses (apologies to you > pathologists out there). Here is a quote I > found somewhere. > > "Ah the Professional Histologist! Walk in awe > when you meet one...Because there are only few > people who can produce something as beautiful > and useful as a slide." > > Drs William Osler and F.E. Mohs > > So smile, put a spring in your step, and at > least acknowledge you co-workers for the good > job they do. If we expect others to respect the > work we do, we must start with ourselves. > (opps, waxing philosophical, sorry) It might > also be a good idea to post this quote > "inconspicuously" around the lab next week. > After all, if the great Dr. F.E. Mohs > recognized our value, maybe the other docs will > consider the idea. :) > > > HAPPY NATIONAL MEDICAL LAB PROFESSIONAL > APPRECIATION WEEK!!! > > > Claire Ingles > UW Hospital > West Clinic Mohs Lab > Madison WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mickie25 <@t> netzero.net Fri Apr 20 09:42:55 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Apr 20 09:43:23 2007 Subject: [Histonet] Happy Friday and National Medical Lab ProfessionalsAppreciation Week In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A12002B@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A12002B@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: Thank you Claire! I was not aware of this quote. I'm sorry I never got to meet Dr. Mohs. I have talked with those who have and they always speak of him with warmth and respect. It is how I feel about Lee Luna and Dezna Sheehan, whom I had the privilege to know. Their inspiration and encouragement helped to make Histotechnology professional. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, April 20, 2007 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Happy Friday and National Medical Lab ProfessionalsAppreciation Week OK, with it being Friday and all. I thought everyone needed a bit of a celebration on making it through another week without killing any pathologists/bosses (apologies to you pathologists out there). Here is a quote I found somewhere. "Ah the Professional Histologist! Walk in awe when you meet one...Because there are only few people who can produce something as beautiful and useful as a slide." Drs William Osler and F.E. Mohs So smile, put a spring in your step, and at least acknowledge you co-workers for the good job they do. If we expect others to respect the work we do, we must start with ourselves. (opps, waxing philosophical, sorry) It might also be a good idea to post this quote "inconspicuously" around the lab next week. After all, if the great Dr. F.E. Mohs recognized our value, maybe the other docs will consider the idea. :) HAPPY NATIONAL MEDICAL LAB PROFESSIONAL APPRECIATION WEEK!!! Claire Ingles UW Hospital West Clinic Mohs Lab Madison WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Fri Apr 20 09:54:56 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Apr 20 09:55:03 2007 Subject: [Histonet] Charlie Brown In-Reply-To: Message-ID: <682906.35948.qm@web55807.mail.re3.yahoo.com> Cannn't resist...resistance is futile. I think I saw him out with Oliver Oil along with the little red-headed girl and Olive Oyl. Roger Moretz It is indeed Friday!!!!!! --- Robyn Vazquez wrote: > I saw him with Snoopy down at the corner store, buy > some ice > cream....happy Friday!!! > > Robyn > > >>> "connie grubaugh" > 4/19/2007 5:14 PM > >>> > > I'm looking for Charlie Brown. Please contact me. > Connie G. > _________________________________________________________________ > Connect to the next generation of MSN Messenger > http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jqb7 <@t> cdc.gov Fri Apr 20 09:56:56 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Apr 20 09:57:01 2007 Subject: [Histonet] Charlie Brown References: <682906.35948.qm@web55807.mail.re3.yahoo.com> Message-ID: What about little Woodstock!? Don't leave him out.... Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Moretz Sent: Friday, April 20, 2007 10:55 AM To: Robyn Vazquez; conniegrubaugh@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Charlie Brown Cannn't resist...resistance is futile. I think I saw him out with Oliver Oil along with the little red-headed girl and Olive Oyl. Roger Moretz It is indeed Friday!!!!!! --- Robyn Vazquez wrote: > I saw him with Snoopy down at the corner store, buy some ice > cream....happy Friday!!! > > Robyn > > >>> "connie grubaugh" > 4/19/2007 5:14 PM > >>> > > I'm looking for Charlie Brown. Please contact me. > Connie G. > _________________________________________________________________ > Connect to the next generation of MSN Messenger > http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timothy.dale.neal <@t> us.army.mil Fri Apr 20 10:01:14 2007 From: timothy.dale.neal <@t> us.army.mil (timothy.dale.neal@us.army.mil) Date: Fri Apr 20 10:01:19 2007 Subject: [Histonet] Charlie Brown In-Reply-To: <682906.35948.qm@web55807.mail.re3.yahoo.com> References: <682906.35948.qm@web55807.mail.re3.yahoo.com> Message-ID: CAP IS HERE VISITING!!!!!!!!!! ----- Original Message ----- From: Roger Moretz Date: Friday, April 20, 2007 9:59 am Subject: Re: [Histonet] Charlie Brown To: Robyn Vazquez , conniegrubaugh@hotmail.com, histonet@lists.utsouthwestern.edu > Cannn't resist...resistance is futile. > > I think I saw him out with Oliver Oil along with the > little red-headed girl and Olive Oyl. > > Roger Moretz > > It is indeed Friday!!!!!! > > > --- Robyn Vazquez wrote: > > > I saw him with Snoopy down at the corner store, buy > > some ice > > cream....happy Friday!!! > > > > Robyn > > > > >>> "connie grubaugh" > > 4/19/2007 5:14 PM > > >>> > > > > I'm looking for Charlie Brown. Please contact me. > > Connie G. > > > _________________________________________________________________ > > Connect to the next generation of MSN Messenger > > > http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Fri Apr 20 10:11:56 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Apr 20 10:11:35 2007 Subject: [Histonet] Charlie Brown In-Reply-To: References: <682906.35948.qm@web55807.mail.re3.yahoo.com> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F07EDCE@bruexchange1.digestivespecialists.com> CAP visiting on a FRIDAY... there should be a law against that! Make sure undated Oliver Oyl isn't lurking in a cupboard. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of timothy.dale.neal@us.army.mil Sent: Friday, April 20, 2007 11:01 AM To: Roger Moretz Cc: histonet@lists.utsouthwestern.edu; conniegrubaugh@hotmail.com Subject: Re: [Histonet] Charlie Brown CAP IS HERE VISITING!!!!!!!!!! ----- Original Message ----- From: Roger Moretz Date: Friday, April 20, 2007 9:59 am Subject: Re: [Histonet] Charlie Brown To: Robyn Vazquez , conniegrubaugh@hotmail.com, histonet@lists.utsouthwestern.edu > Cannn't resist...resistance is futile. > > I think I saw him out with Oliver Oil along with the > little red-headed girl and Olive Oyl. > > Roger Moretz > > It is indeed Friday!!!!!! > > > --- Robyn Vazquez wrote: > > > I saw him with Snoopy down at the corner store, buy > > some ice > > cream....happy Friday!!! > > > > Robyn > > > > >>> "connie grubaugh" > > 4/19/2007 5:14 PM > > >>> > > > > I'm looking for Charlie Brown. Please contact me. > > Connie G. > > > _________________________________________________________________ > > Connect to the next generation of MSN Messenger > > > http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Fri Apr 20 10:29:51 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Apr 20 10:29:55 2007 Subject: [Histonet] Charlie Brown In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F07EDCE@bruexchange1.digestivespecialists.com> Message-ID: <280213.27917.qm@web52501.mail.re2.yahoo.com> I really think the Red Baron got them all.... Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Jason.Wiese <@t> va.gov Fri Apr 20 10:31:07 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri Apr 20 10:31:14 2007 Subject: [Histonet] Top ten stains In-Reply-To: <000001c782e7$132756f0$6400a8c0@D8P565C1> References: <8B07D141BCDE434285DC12B3290E3FB3010239A0@exbackca.caus.dako.net> <000001c782e7$132756f0$6400a8c0@D8P565C1> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E1295E@VHAV20MSGA3.v20.med.va.gov> Okay... I'll chime in.... In order from 1 -10. 1. Diff Quick for HP 2. PAS 3. PAS D. 4. Iron (pearls) 5. Trichrome (Gomori's One step) 6. Alcian Blue 2.5 7. GMS 8. AFB 9. Congo red 10. Trichrome (Masson's) Cheers! Jason -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Thursday, April 19, 2007 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Top ten stains Sarah, Just off the top of my head, without any documentation supporting it (I know, a truly deficient way to operate, lol), I find our top 10 special stains to be: 1. PAS 2. PASD 3. Masson's Trichrome 4. GMS 5. Periodic Acid - Methenamine Silver (PAMS) 6. Iron 7. Congo Red 8. AFB 9. Elastic Van Gieson 10. Alcian Blue / PAS Let us know what you compile as the "Top Ten" Mark -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Jones Sent: Thursday, April 19, 2007 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Top ten stains I am in the process of writing a paper for a class I am taking, and I would appreciate it if you could send me the top ten special stains (not IHC) that you do in your laboratory in the order in which they rank. Thanks in advance for your assistance! Sarah A. Jones, HTL (ASCP) CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vaskomark <@t> yahoo.com Fri Apr 20 10:33:12 2007 From: vaskomark <@t> yahoo.com (Mark Vasko) Date: Fri Apr 20 10:33:16 2007 Subject: [Histonet] Take me off the mailing list Message-ID: <344487.25848.qm@web32410.mail.mud.yahoo.com> Hello, I would like to be taken off the mailing list. Thank you Mark --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From pex0220 <@t> yahoo.com.cn Fri Apr 20 10:34:05 2007 From: pex0220 <@t> yahoo.com.cn (docqian) Date: Fri Apr 20 10:34:11 2007 Subject: [Histonet] alkaline phosphatase antibody Message-ID: <311395.68823.qm@web15201.mail.cnb.yahoo.com> Dear all,=0A=0AI have ordered one antibody-anti-human/mouse/rat alkaline ph= osphatase antibody from one company. I have used it for immunostaining in 1= d or 3d osteoblasts, however, the results are not so nice. It could not lab= el the osteoblast membrane so well since I only find some small vesicles in= cytosol or membrane. But I read one artcile, they showed that alkaline pho= sphatase antibody could label the membane strongly in osteoblasts.=0AIf pos= sible, could you give some advice for this antibody? I am looking forward t= o hearing from you.=0A =0A =0AGuofeng=0A=0A=0A _______________________= ____________________________________ =0A=D1=C5=BB=A2=C3=E2=B7=D1=D3=CA=CF= =E4-3.5G=C8=DD=C1=BF=A3=AC20M=B8=BD=BC=FE =0Ahttp://cn.mail.yahoo.com/From Dorothy.L.Webb <@t> HealthPartners.Com Fri Apr 20 10:34:31 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Apr 20 10:34:36 2007 Subject: [Histonet] Top 10 stains Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640BD7@hpes1.HealthPartners.int> I feel like I am on David Letterman, but, here goes with the Top 10 special stains we do in our lab in order of highest usage first: Giemsa, PAS for fungus, GMS (fungus), Iron, Trichrome, GMS (pneumocystis), Reticulin, Gram, Congo Red, Elastic Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Dorothy.L.Webb <@t> HealthPartners.Com Fri Apr 20 10:37:13 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Apr 20 10:37:19 2007 Subject: [Histonet] OOPS Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640BD8@hpes1.HealthPartners.int> Let me adjust my Top 10 to place AFB as #4 and Fite as #6 and then delete Congo Red and Elastic as being in our "top 10"!! Sorry...shouldn't type and talk on the phone at the same time, I guess!!!!!!!!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From JWEEMS <@t> sjha.org Fri Apr 20 11:32:17 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Apr 20 11:32:42 2007 Subject: [Histonet] Charlie Brown In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF95A8@sjhaexc02.sjha.org> Let us know how it goes! Good luck.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of timothy.dale.neal@us.army.mil Sent: Friday, April 20, 2007 11:01 AM To: Roger Moretz Cc: histonet@lists.utsouthwestern.edu; conniegrubaugh@hotmail.com Subject: Re: [Histonet] Charlie Brown CAP IS HERE VISITING!!!!!!!!!! ----- Original Message ----- From: Roger Moretz Date: Friday, April 20, 2007 9:59 am Subject: Re: [Histonet] Charlie Brown To: Robyn Vazquez , conniegrubaugh@hotmail.com, histonet@lists.utsouthwestern.edu > Cannn't resist...resistance is futile. > > I think I saw him out with Oliver Oil along with the > little red-headed girl and Olive Oyl. > > Roger Moretz > > It is indeed Friday!!!!!! > > > --- Robyn Vazquez wrote: > > > I saw him with Snoopy down at the corner store, buy > > some ice > > cream....happy Friday!!! > > > > Robyn > > > > >>> "connie grubaugh" > > 4/19/2007 5:14 PM > > >>> > > > > I'm looking for Charlie Brown. Please contact me. > > Connie G. > > > _________________________________________________________________ > > Connect to the next generation of MSN Messenger > > > http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pruegg <@t> ihctech.net Fri Apr 20 11:47:41 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Apr 20 11:47:35 2007 Subject: [Histonet] lfigueroaIHCRG Membership Application Form Message-ID: <007401c7836b$9d56f6d0$6501a8c0@Patsy> Verify NSH membership please. _____ From: webmaster@neo.agsci.colostate.edu [mailto:webmaster@neo.agsci.colostate.edu] Sent: Friday, April 20, 2007 10:34 AM To: patsy.ruegg@gmail.com Subject: IHCRG Membership Application Form _____ New_Application: Yes Last_Name: Figueroa First_Name: Lydia NSH_Member: Yes Employer: Sakura FinetekUSA Address: 1047 N Overlook Ridge Rd City: Diamond Bar State: CA Zip: 91765 Province: Country: USA Phone: (800) 7258723 Ext: 7863 Fax: Email: lfigueroa@sakuraus.com Surgical_Pathology: Hematopathology: Orthopedic: Veterinary: Immunology: Plastics: Research: medical device/ industrial- instrument validations studies Paraffin: Yes Frozen: Cytospins: Smears_Touch_Preps: Plastics_Sample: Sample_Other: PAP: Yes APAAP: Yes ABC_HRP: Yes ABC_AP: Yes SA_HRP: Yes SA_AP: Yes IF: IHC_Other: ISH: Gels: PCR: Mol_Other: Automated_IHC: No Company: Select from list IHC_Qualification: No Date_Taken_Exam: Like_To_Take_Exam: Yes Expected_Date: 2008 Can_Provide_Tissues: No Tissues_Provided: Need_Tissues: Yes Tissue_Needed: Endo carcinoma;HBsAg/HBcAg; CMV; EBV. HSV;melanoma;breast tumor ;Hodgkins lymphoma, colon carcinoma, HPV Remote Name: 208.48.224.226 HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1) Date: 04/20/2007 Time: 10:33 AM Other_Tech From slappycraw <@t> yahoo.com Fri Apr 20 11:47:43 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Apr 20 11:47:46 2007 Subject: [Histonet] Looking for Leslie Dixon Message-ID: <415992.54356.qm@web53608.mail.re2.yahoo.com> Hi Leslie if you are out there in histonet land could you e-mail me --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From kimtournear <@t> yahoo.com Fri Apr 20 11:54:58 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Apr 20 11:55:03 2007 Subject: [Histonet] re: top 10 special stains Message-ID: <20070420165458.93006.qmail@web50605.mail.re2.yahoo.com> Where I come from, on Friday,it's: 1. Bud 2. Corona 3. Miller 4. Michelob 5. Bud light 6. Corona with lime 7. Miller light 8. Michelob light 9. Coors (but only if we're really hard up for a beer) 10. Ta-kill-ya shot (usually several) Where I'm at now: I don't do specials "yet".... But I still do the above mentioned from time to time.... Ya'll have a great Friday and a better weekend.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Tucson, AZ ~~ Don't be afraid your life will end, be afraid it will never begin ~~ --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From shive003 <@t> umn.edu Fri Apr 20 12:34:28 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Apr 20 12:34:31 2007 Subject: [Histonet] Toxoplasma and Neospora IHC Message-ID: <012d01c78372$25aca3d0$a1065486@auxs.umn.edu> Hello all, Could someone point me in the direction of where I might be able to obtain IHC-positive control tissues or slides for Toxoplasma gondii and Neospora caninum? I'm having a problem of cross-reactivity with my antibodies and need to optimize their protocols with more positive control cases than what I have on hand. Thanks much for any help that you can provide. Jan Shivers Sr. Scientist/Section Head Histo/IHC/EM Veterinary Diagnostic Laboratory Univ. of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 shive003@umn.edu From Rcartun <@t> harthosp.org Fri Apr 20 12:53:13 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 20 12:53:27 2007 Subject: [Histonet] Toxoplasma and Neospora IHC In-Reply-To: <012d01c78372$25aca3d0$a1065486@auxs.umn.edu> References: <012d01c78372$25aca3d0$a1065486@auxs.umn.edu> Message-ID: <4628C5C902000077000056F5@gwmail.harthosp.org> Hi Jan: I can send you some unstained slides from tissue infected with T. gondii. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jan Shivers" 04/20/07 1:34 PM >>> Hello all, Could someone point me in the direction of where I might be able to obtain IHC-positive control tissues or slides for Toxoplasma gondii and Neospora caninum? I'm having a problem of cross-reactivity with my antibodies and need to optimize their protocols with more positive control cases than what I have on hand. Thanks much for any help that you can provide. Jan Shivers Sr. Scientist/Section Head Histo/IHC/EM Veterinary Diagnostic Laboratory Univ. of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From LewisS <@t> pediatrics.ohio-state.edu Fri Apr 20 12:59:16 2007 From: LewisS <@t> pediatrics.ohio-state.edu (Lewis, Sarah) Date: Fri Apr 20 12:59:40 2007 Subject: [Histonet] TESPA Message-ID: <714B9F12B4E18C4C843B66E8E190F2AD01340B9E@res2k3ms01.CRII.ORG> I have a doc that is looking for a product called TESPA? Evidently it is used to coat slides. The only reference I can find on it is in the Histonet archives from 1999. Is anyone familiar with this product? If so do you know where I can order it? Thanks so much, Sarah Sarah E. Lewis Histotechnician Childrens Research Center for Gene Therapy 700 Childrens Dr Rm WA3112 Columbus OH 43205 (614)-722-2204 LewisS@ccri.net From victor <@t> pathology.washington.edu Fri Apr 20 13:19:52 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Apr 20 13:19:58 2007 Subject: [Fwd: Re: [Histonet] TESPA] Message-ID: <46290448.1020705@pathology.washington.edu> Below is the link if anybody else is interested. -------- Original Message -------- Subject: Re: [Histonet] TESPA Date: Fri, 20 Apr 2007 11:09:05 -0700 From: Victor Tobias To: Lewis, Sarah References: <714B9F12B4E18C4C843B66E8E190F2AD01340B9E@res2k3ms01.CRII.ORG> http://www.ifom-ieo-campus.it/RESEARCH/Mol_path/Final_Protocols/ISH_protocol.pdf In the protocol it mentions Sigma and has the alternate name. Victor Lewis, Sarah wrote: > I have a doc that is looking for a product called TESPA? Evidently it is > used to coat slides. The only reference I can find on it is in the > Histonet archives from 1999. Is anyone familiar with this product? If > so do you know where I can order it? > > Thanks so much, > > Sarah > > > Sarah E. Lewis > Histotechnician > Childrens Research > Center for Gene Therapy > 700 Childrens Dr Rm WA3112 > Columbus OH 43205 > (614)-722-2204 > LewisS@ccri.net > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From laurie.colbert <@t> huntingtonhospital.com Fri Apr 20 13:26:33 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Apr 20 13:26:43 2007 Subject: [Histonet] IHC sausage/checkerboard controls Message-ID: <57BE698966D5C54EAE8612E8941D76831999F2@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can purchase sausage and/or checkerboard controls at a decent price?? Laurie Colbert Huntington Hospital Pasadena, CA From cmiller <@t> physlab.com Fri Apr 20 13:44:39 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Apr 20 13:44:45 2007 Subject: [Histonet] Charlie Brown In-Reply-To: <280213.27917.qm@web52501.mail.re2.yahoo.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F07EDCE@bruexchange1.digestivespecialists.com> <280213.27917.qm@web52501.mail.re2.yahoo.com> Message-ID: <000d01c7837b$f3aa5170$db01a8c0@plab.local> When we find him., I get to hold the football.......Lucy has had her turn! Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Friday, April 20, 2007 10:30 AM To: HistoNet Subject: RE: [Histonet] Charlie Brown I really think the Red Baron got them all.... Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Apr 20 13:53:23 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Apr 20 13:53:29 2007 Subject: [Histonet] Charlie Brown References: <1F937FB30BDB7C4A9F39F83FEA8D379F07EDCE@bruexchange1.digestivespecialists.com><280213.27917.qm@web52501.mail.re2.yahoo.com> <000d01c7837b$f3aa5170$db01a8c0@plab.local> Message-ID: You are one cruel gal! Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, April 20, 2007 2:45 PM To: 'Patricia Adams'; 'HistoNet' Subject: RE: [Histonet] Charlie Brown When we find him., I get to hold the football.......Lucy has had her turn! Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Friday, April 20, 2007 10:30 AM To: HistoNet Subject: RE: [Histonet] Charlie Brown I really think the Red Baron got them all.... Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Fri Apr 20 14:04:46 2007 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Fri Apr 20 14:04:50 2007 Subject: [Histonet] 2007 NSH Awards Deadline Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB10355850B@uwhis-xchng4.uwhis.hosp.wisc.edu> May 1st is the fast approaching application deadline for all 2007 NSH Awards. All available awards/scholarships and their criteria are posted on the NSH website; www.nsh.org NSH members; please take the time to read through the award listings, think about how these awards are applicable to you or to NSH members that you know that deserve to be recognized for their dedicated service to our profession. Then submit applications/nominations and support material before the May 1st deadline. ** If you are continuing your education through college or technical courses, if you want to enhance your knowledge by attending at upcoming NSH meeting - 5 $1000 Educational Scholarships are available for you. ** If you do IHC or In Situ in your laboratory and want to enhance your knowledge in these areas - there are 6 awards totaling $8500 available for you. ** NSH offers awards/scholarships for management, teaching, microwave, foreign travel, hard tissue, outstanding service to NSH, newsletters, websites and more. Feel free to contact me with questions you have about any of the NSH Awards/Scholarship offerings. Jean Mitchell NSH Awards Chairperson 608-263-9184 From pruegg <@t> ihctech.net Fri Apr 20 15:35:10 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Apr 20 15:35:05 2007 Subject: [Histonet] message mistake Message-ID: <00d801c7838b$6486b0f0$6501a8c0@Patsy> Earlier today I accidentally sent a message to histonet that was meant for NSH on membership verification, please disregard this message from me, I hit the wrong email address button, excuse me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From pvalente <@t> sbcglobal.net Fri Apr 20 16:29:03 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Fri Apr 20 16:29:06 2007 Subject: Fw: [Histonet] Top 10 stains Message-ID: <222429.86617.qm@web81702.mail.mud.yahoo.com> Subject: Re: [Histonet] Top 10 stains top ten special stains that I seem to do 1. Pap on my fingertips 2. Gram on the bench and sink 3. PAS on my new white sneakers 4. GMS on the microsope stage 5. Trichrome on the phone reciever 6. Mucicarmine on the computer keyboard 7. AFB on the balance pan 8. Everything on my lab coat\ 9. even more of everthing when I don't wear a lab coat 10. Must remember to do final decolorisation step for all of these. They tried to give me a desk job!! Pat Valente San Antonio ----- Original Message ---- From: "Webb, Dorothy L" To: Histonet@lists.utsouthwestern.edu Sent: Friday, April 20, 2007 10:34:31 AM Subject: [Histonet] Top 10 stains I feel like I am on David Letterman, but, here goes with the Top 10 special stains we do in our lab in order of highest usage first: Giemsa, PAS for fungus, GMS (fungus), Iron, Trichrome, GMS (pneumocystis), Reticulin, Gram, Congo Red, Elastic Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pvalente <@t> sbcglobal.net Fri Apr 20 16:29:03 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Fri Apr 20 16:29:09 2007 Subject: Fw: [Histonet] Top 10 stains Message-ID: <222429.86617.qm@web81702.mail.mud.yahoo.com> Subject: Re: [Histonet] Top 10 stains top ten special stains that I seem to do 1. Pap on my fingertips 2. Gram on the bench and sink 3. PAS on my new white sneakers 4. GMS on the microsope stage 5. Trichrome on the phone reciever 6. Mucicarmine on the computer keyboard 7. AFB on the balance pan 8. Everything on my lab coat\ 9. even more of everthing when I don't wear a lab coat 10. Must remember to do final decolorisation step for all of these. They tried to give me a desk job!! Pat Valente San Antonio ----- Original Message ---- From: "Webb, Dorothy L" To: Histonet@lists.utsouthwestern.edu Sent: Friday, April 20, 2007 10:34:31 AM Subject: [Histonet] Top 10 stains I feel like I am on David Letterman, but, here goes with the Top 10 special stains we do in our lab in order of highest usage first: Giemsa, PAS for fungus, GMS (fungus), Iron, Trichrome, GMS (pneumocystis), Reticulin, Gram, Congo Red, Elastic Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bruce.webber <@t> cefe.cnrs.fr Sat Apr 21 02:52:25 2007 From: bruce.webber <@t> cefe.cnrs.fr (Bruce Webber) Date: Sat Apr 21 02:52:50 2007 Subject: [Histonet] TESPA In-Reply-To: <714B9F12B4E18C4C843B66E8E190F2AD01340B9E@res2k3ms01.CRII.ORG> References: <714B9F12B4E18C4C843B66E8E190F2AD01340B9E@res2k3ms01.CRII.ORG> Message-ID: <4629C2B9.8030601@cefe.cnrs.fr> Hi Sarah, I've just covered a similar issue, but I was given the acronym ATPEs to search for.... As far as I am now aware, both ATPEs and TESPA refer to Silane, officially known as 3-aminopropyl-triethoxysilane. You can get it from Sigma (Cat Ref: A-3648), however, be aware that you can also buy pre-coated slides - Superfrost Plus - that are already coated with silane.... If you do some searching of the Histonet archives, you will turn up many posts debating the pros and cons of Superfrost Plus versus self-coated silane slides! I'll leave it to you to work out which option everyone considers the better slide (I've decided to use the Superfrost Plus for my ISH to begin with and see how good they are)! I hope that helps. Cheers, Bruce On 20/04/2007 6:59 PM, Lewis, Sarah wrote: > I have a doc that is looking for a product called TESPA? Evidently it is > used to coat slides. The only reference I can find on it is in the > Histonet archives from 1999. Is anyone familiar with this product? If > so do you know where I can order it? > > Thanks so much, > > Sarah > > > Sarah E. Lewis > Histotechnician > Childrens Research > Center for Gene Therapy > 700 Childrens Dr Rm WA3112 > Columbus OH 43205 > (614)-722-2204 > LewisS@ccri.net From sally.norton <@t> seattlechildrens.org Sat Apr 21 12:08:24 2007 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Sat Apr 21 12:08:42 2007 Subject: [Histonet] Osmium Message-ID: Hello all, I was wondering if anyone has a method for doing ACE staining that does not use osmium. Thank you, Sally Norton, Histotech Children's Hospital and Regional Medical Center Seattle, Wa CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Shirley_PHUA <@t> hsa.gov.sg Sun Apr 22 13:09:44 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Apr 22 13:10:03 2007 Subject: [Histonet] Shirley is away 23-25 April 2007 ... Message-ID: I will be out of the office from 23-04-2007 to 25-04-2007. I will return on 26 April 2007 (Thursday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From AnthonyH <@t> chw.edu.au Sun Apr 22 18:23:17 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Apr 22 18:25:55 2007 Subject: [Histonet] Osmium & ACE staining Message-ID: Sure do, Method below: Acetylcholinesterase (ACHE) Principle The diagnosis of Hirschsprung's disease is by showing the absence of ganglion cells in the distal portion of the large intestine. Hirschsprung's disease may be divided into 3 main groups: The first and most common, comprises those patients with aganglionosis as far as the rectosigmoid junction (short segment disease). The second group of patients has aganglionosis extending beyond the rectosigmoid junction but not involving the small bowel (long segment disease). The third group which accounts for 2 14% of all Hirschsprung's disease patients have aganglionosis extending into the small bowel, sometimes as far as the duodenum or stomach (total colonic aganglionosis). In both long and short segment disease the appearance in the Acetylcholinesterase preparations will show an increase in nerve fibres present in the muscularis mucosae and this is accompanied by an absence of ganglion cells and an increase in nerve fibres and nerve trunks in the submucosa. This general picture varies with the age of the patient and many clinical factors need to be taken into consideration before a conclusion can be reached. Section Requirements Cut sections at 5 - 6um and stain with toluidine blue. If the appropriate level is obtained then cut 10 sections for the ACHE stain. Solutions 1. 10% buffered formalin cooled to 4oC 2. Dilute Ammonium Sulphide (about 0.005%) Warning: Flammable liquid, Irritant, Toxic stench - see MSDS 20% Ammonium sulphide 20?l Tap water 20ml 3. 0.1% Silver Nitrate 4. Stock solution A Make up 648ml (for 72 tubes) of solution A: Acetylthiocholine iodide (Sigma A5751) 3640mg Sodium Acetate (0.06M) 3.719 g Acetic Acid (0.1M) 0.082ml Sodium Citrate (0.1M) 1.058 g Copper Sulphate (0.03M) 0.540 g Add distilled water up to 633.7ml OMPA (0.004M) (Sigma T-1505) 0.020g Aliquot 9ml into 10ml tubes, labeled "AA" and store at -20oC 5. Stock solution B Make up 72ml of solution B (for 72 tubes): Potassium ferricyanide 5mM (0.005M) 0.119g/72ml distilled water. Aliquot into 1ml tubes, labeled "AB" and store at 20oC. 6. Incubating solution After defrosting, mix one vial of both stock solution A and B. Method 1. Air dry sections for 15 minutes 2. Fix sections in 10%NBF for 10 minutes (at 4?C) 3. Wash well with distilled water 4. Incubate in substrate solution at 37?C for 60 minutes (mix A+B) 5. Wash briefly in distilled water 6. Treat with dilute ammonium sulphide for 30 seconds (in fume hood) 7. Wash in tap water then rinse in distilled water 8. Treat with 0.1% silver nitrate for 1 minute at room temperature 9. Wash in distilled water 10. Counterstain with haematoxylin for 30 seconds 11. Rinse slides in water 12. Dip in blueing solution for 1 minute 13. Rinse quickly in distilled water 14. Dehydrate, clear and mount Results Nerve fibres, ganglion cells, and other tissue containing acetylcholinesterase are stained dark brown to black. (Beware erythrocyte membranes also contain endogenous acetylcholinesterase). References 1. Karnovsky and Roots, J. Histochemistry and Cytochemistry, 1964, Vol 12, p 219 221. 2. Filipe and Lake, Histochemistry in Pathology, 2nd Ed, 1990, p463. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norton, Sally Sent: Sunday, 22 April 2007 3:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osmium Hello all, I was wondering if anyone has a method for doing ACE staining that does not use osmium. Thank you, Sally Norton, Histotech Children's Hospital and Regional Medical Center Seattle, Wa CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From failm <@t> musc.edu Sun Apr 22 21:37:00 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Sun Apr 22 21:37:31 2007 Subject: [Histonet] CD3 clean with RB monos Message-ID: We have had quite a problem with CD3s on bone marrow biopsies being "messy" both with mouse monoclonals and rabbit polyclonals. Protein block is used. Diluting the Ab out further lost some cells in the lymph node control. We tried a rabbit monoclonal. The staining is very specific and intense. The slide is beautifully free of extraneous staining. The higher dilution has not appeared to have effected the number of cells stained. Question is why would the rabbit monoclonal produce a cleaner slide? Rena Fail Rena Fail From langxingpan <@t> pantomics.com Mon Apr 23 03:33:37 2007 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Mon Apr 23 03:33:54 2007 Subject: [Histonet] RE: Vol 41, Issue 33, 'IHC sausage/checkerboard controls' Message-ID: <001601c78582$1735e840$cf94b443@Master> Dear Laurie, We make several IHC control tissue arrays. The most popular one is UNC241 (http://www.pantomics.com/tissuearray/UNC241.htm) that contains 12 different samples in duplicates (24 cores) and can serve as control for >90% of the IHC markers commonly used in routine IHC labs. Some IHC reagent companies such as LabVision have used our control arrays for their in-house IHC QA/QC. Routine IHC labs, such as IHC Division of UK NEQAS and Immunostains Lab of MAYO Clinic (Rochester) have also purchased our control arrays for their routine IHC. If you like to try, we can send you some free samples. We have a special offer for routine IHC labs. Regards, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco CA 94107, U.S.A. Direct line: 1-510-207-9788 Fax: 1-510-653-1227 www.pantomics.com Message: 5 Date: Fri, 20 Apr 2007 11:26:33 -0700 From: "Laurie Colbert" Subject: [Histonet] IHC sausage/checkerboard controls To: Message-ID: <57BE698966D5C54EAE8612E8941D76831999F2@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="iso-8859-1" Does anyone know where I can purchase sausage and/or checkerboard controls at a decent price?? Laurie Colbert Huntington Hospital Pasadena, CA From ree3 <@t> leicester.ac.uk Mon Apr 23 03:40:36 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Apr 23 03:40:41 2007 Subject: [Histonet] Looking for Leslie Dixon In-Reply-To: <415992.54356.qm@web53608.mail.re2.yahoo.com> References: <415992.54356.qm@web53608.mail.re2.yahoo.com> Message-ID: She's probably with Charlie Brown?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: 20 April 2007 17:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for Leslie Dixon Hi Leslie if you are out there in histonet land could you e-mail me --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhanna <@t> histosearch.com Mon Apr 23 06:05:50 2007 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Mon Apr 23 06:05:56 2007 Subject: [Histonet] CD3 clean with RB monos In-Reply-To: References: Message-ID: <44738AE0-CFAC-4C89-A6DB-678E211A9B96@histosearch.com> Hi Rena, I think it mainly has to do with a rabbit antibody's greater affinity for human tissue versus mouse antibodies. We seem to be closer to rabbits on an evolutionary scale than mice. Up until recently, all rabbit antibodies have been made by injecting rabbits with the antigen, which produces polyclonal antibodies, a mixture of antibodies targeting that antigen. A few years ago, researchers developed the technology to manufacture rabbit monoclonal antibodies using hybridomas, the method used to manufacture mouse monoclonals, which produces antibodies with greater specificity, a single clone. So, a rabbit monoclonal can have the same specificity as a mouse monoclonal, with up to a 10 times greater affinity for it's human target. I've visited a few hundred IHC labs over the last few years and utilized rabbit monoclonals in many of them. Consistently, histotechs and pathologists commented on the cleaner backgrounds and stronger intensity with them. I assume the greater affinity for human targets means a less affinity for anything that is not the intended target, creating less background. The promise of a new round of antibodies with a 10 times greater affinity for their targets could be a big benefit for IHC labs and cancer patients. There is also research work going on to use rabbit monoclonals therapeutically. With the successes so far in IHC, it makes you wonder if drugs like Genetech's Herceptin, which is a mouse monoclonal that blocks the her2 gene and has been shown to double the chance of survival of patients who take it, could be even more successful as a rabbit monoclonal. Best Regards, Marvin Hanna On Apr 22, 2007, at 9:37 PM, Mildred Fail wrote: > We have had quite a problem with CD3s on bone marrow biopsies being > "messy" both with mouse monoclonals and rabbit polyclonals. Protein > block is used. Diluting the Ab out further lost some cells in the > lymph > node control. We tried a rabbit monoclonal. The staining is very > specific and intense. The slide is beautifully free of extraneous > staining. The higher dilution has not appeared to have effected the > number of cells stained. Question is why would the rabbit > monoclonal produce a cleaner slide? > Rena Fail > > Rena Fail > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bruce.webber <@t> cefe.cnrs.fr Mon Apr 23 06:39:04 2007 From: bruce.webber <@t> cefe.cnrs.fr (Bruce Webber) Date: Mon Apr 23 06:39:56 2007 Subject: [Histonet] Help with ISH on ants - last request! In-Reply-To: <461D1D76.8050409@cefe.cnrs.fr> References: <46151805.8020602@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8750@LMMAIL.lmhosp.local> <461BBB0A.8040609@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8753@LMMAIL.lmhosp.local> <461D1A1E.7020709@cefe.cnrs.fr> <461D1D76.8050409@cefe.cnrs.fr> Message-ID: <462C9AD8.2060805@cefe.cnrs.fr> My last request for help! Please! I am still yet to receive any responses on any of the issues outlined below.... even advice on only small parts of the points mentioned would be most appreciated. Cheers, Bruce On 05/04/2007 16:38, Bruce Webber wrote: Hi Histonetters, I am looking for advice on preparing and processing small (c. 2-3mm long) adult ants (i.e. with a well-formed chitinous exoskeleton) for in situ hybridization (ISH) detection of bacteria (using DNA probes to target RNA). The ants are transverse sectioned into 3 parts before fixing to allow greater penetration into each of the 3 main body cavities. I've trawled the archives for various options and have consulted my histological mentor, Bruce Abaloz, but after not finding what I was looking for, I would greatly appreciate advice, opinions and recommendations from others. I do promise to post a summary of any responses together with any methodological tips I found along the way! The main problem I see is dealing with the cuticle to allow for good sectioning (5-8um), but using chemicals & methods that don't interfere with ISH, damage the target RNA, or interfere with the efficiency of the ISH probes. My understanding is that the following factors need to be addressed: 1) Fixing the tissue: The only available material available at this stage was fixed in 2% gluteraldehyde (12h at 4?C) and then stored (> 3 weeks) in 80% EtOH (meaning that gut excision is not an option due to brittle material). In the future, 4% paraformaldehyde, 10% neutral buffered formalin or just straight into 70% EtOH (with slightly shorter storage times) could also be considered as options if people feel that they may be better (the latter option would certainly increase the availability of material). 2) Softening the cuticle: IMHO this is the biggest potential area for chemical damage/interference with ISH. Proposals I am aware of that would not be compatible with ISH due to acidic damage of the RNA are [1] Perenyi's fluid (4:3:3, 10% nitric acid:100% EtOH:0.05% chromic acid); [2] Diaphanol (50% glacial acetic acid saturated with ClO2); and [3] Bouin's fixative (3:1:2, saturated picric acid:formaldehyde:glacial acetic acid). However, other suggestions from previous posts which I know very little about include [4] chloral hydrate processing (after tissue dehydration, melt equal parts of chloral hydrate & phenol and immerse tissue for up to 7 days, followed by chloroform as an intermediary agent); [5] Mollifex Gurr (glycerol, phenol, acetone and EtOH solution, applied to the cutting surface of the paraffin block); [6] Nair (as in the hair removal cream - thioglycolate salts & calcium hydroxide, between fixing and processing, soak the tissue for c. 24hrs); and [7] Phenol (after fixation, soak fixed specimens in 4% phenol (in 80% EtOH) for 24 hours). Lastly, there is 1% DMSO (added to the fixative), but given that the ant is chopped into 3 sections, this may not be necessary to ensure adequate penetration (but does DMSO also improve sectioning?). 3) Processing the tissue: The ongoing debate of xylene v histoclear (or histosolve)..... Histoclear is probably safer, but does xylene produce better results on tissue for ISH? Has anyone had experience with either on similar invertebrate material? Others have suggested using isopropyl alcohol (instead of EtOH) for dehydration as is tends to have a less hardening effect on the tissue. 4) Material for embedding: I've seen it argued that tougher material such as that containing chitinous exoskeletons benefits from using a harder embedding medium (e.g. TissuePrep 2 from Fisher) or one with better infiltration (e.g. Paraplast X-tra) over standard paraffin. I've also read that Paraplast Plus (containing DMSO) makes sectioning of cuticles easier. Any opinions/recommendations/experience with similar material and any of these embedding mediums would be appreciated! Thank you all in advance for your help with this matter (I apologise for the length of this post!). Bruce Webber Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, France (Key words: cuticle, exoskeleton, chitin, insect, invertebrate, ant, in situ hybridization, ISH, softening) From Jessica.Vacca <@t> HCAhealthcare.com Mon Apr 23 09:18:19 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Apr 23 09:18:27 2007 Subject: [Histonet] Happy Lab Week To all! Message-ID: <41E16A15CE78374EA45B57E0F94339B8024A4794@ORLEV01.hca.corpad.net> I hope everyone has a great week.....Thanks for all you do! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From olek.michalski <@t> nencki.gov.pl Mon Apr 23 09:29:56 2007 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Mon Apr 23 09:28:30 2007 Subject: [Histonet] freezing microtome Message-ID: Dear Histonetters, I've just found a freezing microtome in our laboratory. It's Microm HM 440E w/ K400 fast freezing unit. I'd like to get it running but can't find a piece of manual. Our technical lady claims that the freezing unit haven't ever been used. I don't like to break it by improper operation, so could anybody tell me how to do the test run and what to check before? Another issue: I've noticed that there is a large metal clamp inside the knife holder with a screw connector. I suppose it is for cooling the knife and I should connect the clamp to the cooling stage. The problem is I have no idea how the connector look like and I have to find it in the large box full of parts. If I eventually find it, will it work efficiently? I'd like to cut large pieces of brain about 15mm thick into 40um sections frozen to -30 C deg. Do anybody have such experience? Best regards Olek Michalski -- .LP` a, dd' !4aPLa _J'4;. . . Laboratory of Neurobiology -!L?!9w_a, ?0L,"'. of Development and Evolution ?l -\aJ'aP' j\aas j1 Nencki Institute of Experimental Biology _dW~ "ajl ul. Pasteura 3, 02-093 Warszawa, Poland _#' -\L Tel. +48 22 5892268, Fax +48 22 8225342 "P, "J_ From Karen.Heckford <@t> CHW.edu Mon Apr 23 10:23:50 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Mon Apr 23 10:23:57 2007 Subject: [Histonet] Lab Week Message-ID: Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE From pruegg <@t> ihctech.net Mon Apr 23 10:47:41 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Mon Apr 23 14:22:54 2007 Subject: [Histonet] Help with ISH on ants - last request! In-Reply-To: <462C9AD8.2060805@cefe.cnrs.fr> References: <46151805.8020602@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8750@LMMAIL.lmhosp.local> <461BBB0A.8040609@cefe.cnrs.fr> <59688E3227A2204CBC05459074547F951C8753@LMMAIL.lmhosp.local> <461D1A1E.7020709@cefe.cnrs.fr> <461D1D76.8050409@cefe.cnrs.fr> <462C9AD8.2060805@cefe.cnrs.fr> Message-ID: <1360.71.219.229.176.1177343261.squirrel@71.219.229.176> Bruce, I have been working on doing IHC in glycol methacrylate processed tissue (GMA) which might be of use to you, we have even done some inblock labeling of wet tissue and then processed it into GMA which is much harder than paraffin, we used it to cut undecalcified bone. It can be very difficult to get large molecule IHC/ISh reagents into GMA sections as it cannot be removed before staining, but if you do the labeling in the wet tissue you see the signal already there when you cut the section, another option would be to use methyl methacrylate (MMA) which can be removed before staining. I am out of town but contact me directly for some protocols if you are interested in trying GMA processing for this. Patsy > My last request for help! Please! I am still yet to receive any > responses on any of the issues outlined below.... even advice on only > small parts of the points mentioned would be most appreciated. > > Cheers, Bruce > > > On 05/04/2007 16:38, Bruce Webber wrote: > Hi Histonetters, > > I am looking for advice on preparing and processing small (c. 2-3mm > long) adult ants (i.e. with a well-formed chitinous exoskeleton) for in > situ hybridization (ISH) detection of bacteria (using DNA probes to > target RNA). The ants are transverse sectioned into 3 parts before > fixing to allow greater penetration into each of the 3 main body cavities. > > I've trawled the archives for various options and have consulted my > histological mentor, Bruce Abaloz, but after not finding what I was > looking for, I would greatly appreciate advice, opinions and > recommendations from others. I do promise to post a summary of any > responses together with any methodological tips I found along the way! > > The main problem I see is dealing with the cuticle to allow for good > sectioning (5-8um), but using chemicals & methods that don't interfere > with ISH, damage the target RNA, or interfere with the efficiency of the > ISH probes. My understanding is that the following factors need to be > addressed: > > 1) Fixing the tissue: > The only available material available at this stage was fixed in 2% > gluteraldehyde (12h at 4?C) and then stored (> 3 weeks) in 80% EtOH > (meaning that gut excision is not an option due to brittle material). > In the future, 4% paraformaldehyde, 10% neutral buffered formalin or > just straight into 70% EtOH (with slightly shorter storage times) could > also be considered as options if people feel that they may be better > (the latter option would certainly increase the availability of material). > > 2) Softening the cuticle: > IMHO this is the biggest potential area for chemical damage/interference > with ISH. Proposals I am aware of that would not be compatible with ISH > due to acidic damage of the RNA are [1] Perenyi's fluid (4:3:3, 10% > nitric acid:100% EtOH:0.05% chromic acid); [2] Diaphanol (50% glacial > acetic acid saturated with ClO2); and [3] Bouin's fixative (3:1:2, > saturated picric acid:formaldehyde:glacial acetic acid). > > However, other suggestions from previous posts which I know very little > about include [4] chloral hydrate processing (after tissue dehydration, > melt equal parts of chloral hydrate & phenol and immerse tissue for up > to 7 days, followed by chloroform as an intermediary agent); [5] > Mollifex Gurr (glycerol, phenol, acetone and EtOH solution, applied to > the cutting surface of the paraffin block); [6] Nair (as in the hair > removal cream - thioglycolate salts & calcium hydroxide, between fixing > and processing, soak the tissue for c. 24hrs); and [7] Phenol (after > fixation, soak fixed specimens in 4% phenol (in 80% EtOH) for 24 > hours). Lastly, there is 1% DMSO (added to the fixative), but given > that the ant is chopped into 3 sections, this may not be necessary to > ensure adequate penetration (but does DMSO also improve sectioning?). > > 3) Processing the tissue: > The ongoing debate of xylene v histoclear (or histosolve)..... > Histoclear is probably safer, but does xylene produce better results on > tissue for ISH? Has anyone had experience with either on similar > invertebrate material? Others have suggested using isopropyl alcohol > (instead of EtOH) for dehydration as is tends to have a less hardening > effect on the tissue. > > 4) Material for embedding: > I've seen it argued that tougher material such as that containing > chitinous exoskeletons benefits from using a harder embedding medium > (e.g. TissuePrep 2 from Fisher) or one with better infiltration (e.g. > Paraplast X-tra) over standard paraffin. I've also read that Paraplast > Plus (containing DMSO) makes sectioning of cuticles easier. Any > opinions/recommendations/experience with similar material and any of > these embedding mediums would be appreciated! > > Thank you all in advance for your help with this matter (I apologise for > the length of this post!). > > Bruce Webber > Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, France > (Key words: cuticle, exoskeleton, chitin, insect, invertebrate, ant, in > situ hybridization, ISH, softening) > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From amylee779 <@t> yahoo.com Mon Apr 23 14:01:07 2007 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Apr 23 14:27:53 2007 Subject: [Histonet] Two questions about double IHC Message-ID: <956641.56478.qm@web38010.mail.mud.yahoo.com> Hello, I need to do double IHC on FFPE mouse tissue. Unluckily two antibodies I have to use are all rat anti-mouse. The protocol for these antibodies are very similar: Pro K digestion, Primary antibody, Rb anti-Rat 2ND antibody and the rest is following PerkinElmer TSA biotin system instruction (Streptavidin-HRP, biotinylated-tyramide and Streptavidin-HRP). I will perform the two IHC sequentially. I have two questions: 1. Do I need to do Proteinase K for the second antigen? 2. Can I keep all other steps the same and just use Streptavidin-FITC or Streptavidin-Texas Red at the final step to differentiate two antigen? BTW, could you recommend a good website to learn double IHC? Any input is highly appreciated! Amy --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From TMcNemar <@t> lmhealth.org Mon Apr 23 10:42:20 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Apr 23 14:28:46 2007 Subject: [Histonet] Lab Week In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F488@lmhsmail.lmhealth.org> Labweek used to be a huge deal around here. We had potlucks, games, pizza party, etc., all day, every day. It really got to be way too much. All that changed a couple of years ago and now the hospital has a week-long celebration for all departments instead of each department celebrating their own weeks throughout the year. Funny thing though, we still celebrate nursing days..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, April 23, 2007 11:24 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE From japoteete <@t> saintfrancis.com Mon Apr 23 10:42:47 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Mon Apr 23 14:39:09 2007 Subject: [Histonet] Lab Week In-Reply-To: Message-ID: Evidently the vendors around here haven't heard about a rule, because we are having something "vendor-provided" every day this week. They volunteered, and we didn't solicit anything from them. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, April 23, 2007 10:24 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE From hymclab <@t> hyhc.com Mon Apr 23 14:56:25 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Mon Apr 23 14:48:07 2007 Subject: [Histonet] Lab Week Message-ID: This week we are having: Monday: Repungent food day (Pot Luck with foods with gross names,ie. Chunky cat barf(spaghetti squash with cottage cheese and spaghetti sauce), blood clot cake, vomit dip, rancid mucous(guacamole) etc.... Tuesday: Sub sandwchiches provided for by the Pathologists Wednesday: Breakfast (not sure who is providing that) Thurs: Chinese take out Friday: Cake and punch (provided by our Lab Director) All week we have contests: Guess who's hands (several people had their pictures taken of their hands and put on a bulletin board), Scavenger hunt (clues with little pieces of paper in hidden spots like Clue: Look for body parts. They were hidden in the cupboard we store our buckets of tissues. Dress up lab coat day (had some old lab coats and people could put whatever they want on them). We also had a word search puzzle. Hope everyone has a great lab week!! Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Monday, April 23, 2007 10:42 AM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Week Labweek used to be a huge deal around here. We had potlucks, games, pizza party, etc., all day, every day. It really got to be way too much. All that changed a couple of years ago and now the hospital has a week-long celebration for all departments instead of each department celebrating their own weeks throughout the year. Funny thing though, we still celebrate nursing days..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, April 23, 2007 11:24 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From burch007 <@t> mc.duke.edu Mon Apr 23 15:09:52 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Mon Apr 23 15:09:58 2007 Subject: [Histonet] CD3 clean with RB monos In-Reply-To: Message-ID: Mildred: Check out www.epitomics.com for information on rabbit monoclonal technology. Best Regards, Lemuel Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Mildred Fail" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/22/2007 09:37 PM To cc Subject [Histonet] CD3 clean with RB monos We have had quite a problem with CD3s on bone marrow biopsies being "messy" both with mouse monoclonals and rabbit polyclonals. Protein block is used. Diluting the Ab out further lost some cells in the lymph node control. We tried a rabbit monoclonal. The staining is very specific and intense. The slide is beautifully free of extraneous staining. The higher dilution has not appeared to have effected the number of cells stained. Question is why would the rabbit monoclonal produce a cleaner slide? Rena Fail Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwstarbu <@t> mdanderson.org Mon Apr 23 11:05:40 2007 From: mwstarbu <@t> mdanderson.org (mwstarbu@mdanderson.org) Date: Mon Apr 23 15:15:27 2007 Subject: [Histonet] tissue processing Message-ID: Hello, Is there a standard tissue volume to liquid volume ratio that you use when dehydrating samples with ethanol? Thank you in advance. Mike From koellingr <@t> comcast.net Mon Apr 23 15:35:46 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Apr 23 15:35:53 2007 Subject: [Histonet] CD3 clean with RB monos Message-ID: <042320072035.7880.462D18A20006A21C00001EC822070215739D09020704040A0105@comcast.net> Rena, I agree that the epitomics website is great for learning about this technology and I further agree it could be of help as has been mentioned before. I am a bit skeptical of claims of a 10-fold better affinity than mouse monoclonals as a blanket statement. At a previous meeting a while back we asked about claims of higher affinity and couldn't get a sufficient (to our mind) response. To me that response would be in the form of a side to side comparison, using the same immunogen, and with a host mouse and rabbit side by side each producing monoclonals to same target to see which might be better. I believe the concept of a superior rabbit monoclonal technology in terms of being able to break immunological tolerance in the immunized systems, especially for some difficult targets, is probably right on. But simply breaking tolerance is not the same as producing higher affinity antibodies. For the blanket statement that rabbit monoclonals have higher affinity, I'd like to see data comparing them to their (identical) target in a mouse host and see data such as from Biacore, x-ray crystallography and that differences in somatic hypermutation are indeed making these higher affinity. That being said, I agree that the rabbit monoclonals have great use and even more potential. I've used several (many) that are far superior to their counter-part mouse monoclonals. However, I've used (and heard of) a few that weren't as good. So while I like rabbit monoclonals, use them, advocate for them and endorse the suggestion you try them, I'm not convinced that as a rule they have 10x higher affinity than do mouse monoclonals. Ray Koelling Phenopath Laboratories Seattle, WA -------------- Original message ---------------------- From: "Mildred Fail" > We have had quite a problem with CD3s on bone marrow biopsies being > "messy" both with mouse monoclonals and rabbit polyclonals. Protein > block is used. Diluting the Ab out further lost some cells in the lymph > node control. We tried a rabbit monoclonal. The staining is very > specific and intense. The slide is beautifully free of extraneous > staining. The higher dilution has not appeared to have effected the > number of cells stained. Question is why would the rabbit > monoclonal produce a cleaner slide? > Rena Fail > > Rena Fail > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Mon Apr 23 15:40:09 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Apr 23 15:40:21 2007 Subject: [Histonet] Lab Week In-Reply-To: References: Message-ID: <8C953FEB7AD3A80-1524-42AC@WEBMAIL-RB18.sysops.aol.com> This week we are Monday-------create your own sunday Tuesday------finger food munchies all day Wednesday---lunch from the pathologists Thursday-----death by chocolate day Friday--------covered dish Plus we have a raffle everyday and we do give-aways everyday, small give-aways, but give-aways nontheless. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: Karen.Heckford@CHW.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, 23 Apr 2007 11:23 AM Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From koellingr <@t> comcast.net Mon Apr 23 16:32:56 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Apr 23 16:33:00 2007 Subject: [Histonet] CD3 clean with RB monos Message-ID: <042320072132.3190.462D26080006F90200000C7622070032019D09020704040A0105@comcast.net> PS. As far as I know Herceptin (trastuzumab) is a HUMANIZED monoclonal antibody and not a mouse or rabbit monoclonal. While certainly many things are possible, I'm skeptical of rabbit monoclonals, no matter how great they are for IHC, going into human therapeutics in light of the norm which is to humanize these reagents. Unless you were to "humanize" the rabbit monoclonal itself similar to humanizing mouse monoclonals. Ray Koelling Phenopath Laboratories Seattle, WA -------------- Original message ---------------------- From: koellingr@comcast.net > Rena, > > I agree that the epitomics website is great for learning about this technology > and I further agree it could be of help as has been mentioned before. I am a > bit skeptical of claims of a 10-fold better affinity than mouse monoclonals as a > blanket statement. > > At a previous meeting a while back we asked about claims of higher affinity and > couldn't get a sufficient (to our mind) response. To me that response would be > in the form of a side to side comparison, using the same immunogen, and with a > host mouse and rabbit side by side each producing monoclonals to same target to > see which might be better. I believe the concept of a superior rabbit > monoclonal technology in terms of being able to break immunological tolerance in > the immunized systems, especially for some difficult targets, is probably right > on. > But simply breaking tolerance is not the same as producing higher affinity > antibodies. > > For the blanket statement that rabbit monoclonals have higher affinity, I'd like > to see data comparing them to their (identical) target in a mouse host and see > data such as from Biacore, x-ray crystallography and that differences in somatic > hypermutation are indeed making these higher affinity. > That being said, I agree that the rabbit monoclonals have great use and even > more potential. I've used several (many) that are far superior to their > counter-part mouse monoclonals. However, I've used (and heard of) a few that > weren't as good. > So while I like rabbit monoclonals, use them, advocate for them and endorse the > suggestion you try them, I'm not convinced that as a rule they have 10x higher > affinity than do mouse monoclonals. > > Ray Koelling > Phenopath Laboratories > Seattle, WA > > -------------- Original message ---------------------- > From: "Mildred Fail" > > We have had quite a problem with CD3s on bone marrow biopsies being > > "messy" both with mouse monoclonals and rabbit polyclonals. Protein > > block is used. Diluting the Ab out further lost some cells in the lymph > > node control. We tried a rabbit monoclonal. The staining is very > > specific and intense. The slide is beautifully free of extraneous > > staining. The higher dilution has not appeared to have effected the > > number of cells stained. Question is why would the rabbit > > monoclonal produce a cleaner slide? > > Rena Fail > > > > Rena Fail > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Apr 23 10:53:22 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Apr 23 17:08:19 2007 Subject: [Histonet] Lab Week In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70F6@EMAIL.archildrens.org> Happy Lab Week to All!!! We have different sections of the lab responsible for one day of lab week. Today Virology and Microbiology provided breakfast. Hospital administration is providing lunch. Tomorrow we have a company providing lunch but they are doing an inservice as well. Wednesday we have breakfast provided by chemistry and lunch provided by a company plus an inservice during the lunch. Thursday we have another company sponsored inservice lunch. Friday we are having sundaes sponsored by one of my techs who won this gift at our state meeting from a vendor. We also play games all week with prizes. The hospital also gives everyone a gift. We have a great time and everyone always looks forward to lab week! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, April 23, 2007 10:24 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Lchausse <@t> nmh.org Mon Apr 23 17:26:01 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Mon Apr 23 17:26:34 2007 Subject: [Histonet] Lab Week In-Reply-To: <8C953FEB7AD3A80-1524-42AC@WEBMAIL-RB18.sysops.aol.com> Message-ID: We had a Lab Coat decorating contest that was judged today - staff decorated white, disposable lab coats last week and submitted them on Friday. It seemed to be quite successful. There was a 1st, 2nd, and 3rd place as well as three honorable mentions. We also had an ice cream social today for the staff. The Lab Week Committee scheduled three CE presentations by various physicians and arranged for PACE credits. There will be a staff bake-off tomorrow - with prizes for various categories. That was very successful last year. We also have lunch / dinner one day this week for the staff and laboratory tours. Puzzles & word searches were distributed last week and there will be prizes for those activities as well. Prizes are primarly donated by staff and managers. Actually, we're lucky as all of the activities were handled by our Lab Week Committee and they really did a great job this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, April 23, 2007 3:40 PM To: Karen.Heckford@CHW.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week This week we are Monday-------create your own sunday Tuesday------finger food munchies all day Wednesday---lunch from the pathologists Thursday-----death by chocolate day Friday--------covered dish Plus we have a raffle everyday and we do give-aways everyday, small give-aways, but give-aways nontheless. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: Karen.Heckford@CHW.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, 23 Apr 2007 11:23 AM Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From bwhitaker <@t> brownpathology.com Mon Apr 23 18:18:09 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Mon Apr 23 18:17:05 2007 Subject: [Histonet] Lab Week In-Reply-To: <8C953FEB7AD3A80-1524-42AC@WEBMAIL-RB18.sysops.aol.com> Message-ID: <001101c785fd$a8560be0$3601a8c0@brownpathology.net> Today we had breakfast provided by our executive director Tuesday-- lab provided "gift" handed out to everyone Wednesday--salad day (supervisors provide an assortment of salads) Thursday-- give away day (raffles, etc.) Friday-- pathologists provide lunch Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, April 23, 2007 3:40 PM To: Karen.Heckford@CHW.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week This week we are Monday-------create your own sunday Tuesday------finger food munchies all day Wednesday---lunch from the pathologists Thursday-----death by chocolate day Friday--------covered dish Plus we have a raffle everyday and we do give-aways everyday, small give-aways, but give-aways nontheless. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: Karen.Heckford@CHW.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, 23 Apr 2007 11:23 AM Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Apr 23 18:27:15 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Apr 23 19:04:24 2007 Subject: [Histonet] Help with ISH on ants Message-ID: <20070423232715.26794.qmail@web50109.mail.re2.yahoo.com> Hello Bruce, 1st the glut fixed tissue is probably not good for ISH or IHC. You can use them to maybe practice cutting. I have done drosophila for IHC by fixing in an alcoholic formalin. The alcohol helps with faster penetration and better fixation. When processed to paraffin, face off the block, soak in ammonia water about 5-10 minutes, move knife to new edge, chill block, and cut. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From jnocito <@t> satx.rr.com Mon Apr 23 19:50:40 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Apr 23 19:50:41 2007 Subject: [Histonet] Lab Week References: <001101c785fd$a8560be0$3601a8c0@brownpathology.net> Message-ID: <007501c7860a$9506e340$d49eae18@yourxhtr8hvc4p> we hit up our vendors. Monday- pizza lunch Tuesday- breakfast tacos Wednesday- lunch Thursday- breakfast & ice cream social Friday- pathologists buy lunch Saturday- we all go to Weight Watchers JTT ----- Original Message ----- From: "Bonnie Whitaker" To: Sent: Monday, April 23, 2007 6:18 PM Subject: RE: [Histonet] Lab Week Today we had breakfast provided by our executive director Tuesday-- lab provided "gift" handed out to everyone Wednesday--salad day (supervisors provide an assortment of salads) Thursday-- give away day (raffles, etc.) Friday-- pathologists provide lunch Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, April 23, 2007 3:40 PM To: Karen.Heckford@CHW.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week This week we are Monday-------create your own sunday Tuesday------finger food munchies all day Wednesday---lunch from the pathologists Thursday-----death by chocolate day Friday--------covered dish Plus we have a raffle everyday and we do give-aways everyday, small give-aways, but give-aways nontheless. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: Karen.Heckford@CHW.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, 23 Apr 2007 11:23 AM Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laurie <@t> conxis.com Mon Apr 23 21:22:07 2007 From: Laurie <@t> conxis.com (Laurie Popp) Date: Mon Apr 23 21:23:50 2007 Subject: [Histonet] RE: Histonet Digest, Vol 41, Issue 35 Message-ID: <000001c78617$5b7591f0$9700a8c0@laurie> We're celebrating lab week :-) I've been on the committee twice and hopefully next year we'll get an earlier start. The supervisors and pathologists are feeding us ice cream sundaes tomorrow (they're making them... )and we're having healthy potluck tomorrow and junk food potluck on Thursday... Wings on Wednesday ... And one of the techs came up with a histology sudoku and crossword puzzle and we're doing a name this pet's owner contest ... Institution wide they did a door decorating contest and they are having coffee for everyone. Since we are also not allowed to accept vendor gifts we had a budget to go shopping so I picked up a few little things at our local cost plus world market and we have a way up our sleeves to get around the gift card rule :-) Happy Lab Week everyone :-) Laurie Popp HT Candidate, Mayo Clinic Rochester Message: 10 Date: Mon, 23 Apr 2007 08:23:50 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Lab Week To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 41, Issue 35 **************************************** From mhanna <@t> histosearch.com Tue Apr 24 01:32:08 2007 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Tue Apr 24 01:32:16 2007 Subject: [Histonet] CD3 clean with RB monos In-Reply-To: <042320072132.3190.462D26080006F90200000C7622070032019D09020704040A0105@comcast.net> References: <042320072132.3190.462D26080006F90200000C7622070032019D09020704040A0105@comcast.net> Message-ID: <5EFF2B22-029D-460C-95D9-40CD48854757@histosearch.com> Hi Ray, Thanks for the additional input. I stand corrected. Herceptin is a HUMANIZED mouse monoclonal antibody. From Dr. Kimball's online Biology textbook, "The [Humanized] antibody combines only the amino acids responsible for making the antigen binding site (the hypervariable regions) of a mouse (or rat) antibody with the rest of a human antibody molecule thus replacing its own hypervariable regions." This helps reduce the problem of HAMA (human anti-mouse antibodies), which can cause damage to the kidneys and cause the therapeutic antibodies to be quickly eliminated from the human patient. http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/ Monoclonals.html I will also qualify my statement, "with up to a 10 times greater affinity" with "according to a number of IHC vendors of rabbit monoclonal antibodies". I have found at least 4 making claims of higher affinity. The Epitomics website claims that mouse monoclonals have affinities of "Nanomolar (~10-9 KD M)" and rabbit monoclonals have affinities of "Picomolar (10-12 KD M) possible", a thousand fold potential increase. I did not know that there are researchers who disagree with these statements and appreciate you pointing it out. I agree additional research should be done. http://www.epitomics.com/technology/tech.html According to a news release on the Epitomics website, they are providing humanized antibodies for therapeutic research. It is early to speculate that (possible) higher affinities of humanized rabbit monoclonals will provide a better therapeutic response in patients compared to humanized mouse monoclonals and will require many years of research and clinical trials to determine. I also agree I have used rabbit monoclonal antibodies in IHC that have not given better results, and sometimes worse results, than their mouse monoclonal counterparts. Best Regards, Marvin Hanna mhanna@histosearch.com On Apr 23, 2007, at 4:32 PM, koellingr@comcast.net wrote: > PS. > As far as I know Herceptin (trastuzumab) is a HUMANIZED monoclonal > antibody and not a mouse or rabbit monoclonal. While certainly > many things are possible, I'm skeptical of rabbit monoclonals, no > matter how great they are for IHC, going into human therapeutics in > light of the norm which is to humanize these reagents. Unless you > were to "humanize" the rabbit monoclonal itself similar to > humanizing mouse monoclonals. > > Ray Koelling > Phenopath Laboratories > Seattle, WA > -------------- Original message ---------------------- > From: koellingr@comcast.net >> Rena, >> >> I agree that the epitomics website is great for learning about >> this technology >> and I further agree it could be of help as has been mentioned >> before. I am a >> bit skeptical of claims of a 10-fold better affinity than mouse >> monoclonals as a >> blanket statement. >> >> At a previous meeting a while back we asked about claims of higher >> affinity and >> couldn't get a sufficient (to our mind) response. To me that >> response would be >> in the form of a side to side comparison, using the same >> immunogen, and with a >> host mouse and rabbit side by side each producing monoclonals to >> same target to >> see which might be better. I believe the concept of a superior >> rabbit >> monoclonal technology in terms of being able to break >> immunological tolerance in >> the immunized systems, especially for some difficult targets, is >> probably right >> on. >> But simply breaking tolerance is not the same as producing higher >> affinity >> antibodies. >> >> For the blanket statement that rabbit monoclonals have higher >> affinity, I'd like >> to see data comparing them to their (identical) target in a mouse >> host and see >> data such as from Biacore, x-ray crystallography and that >> differences in somatic >> hypermutation are indeed making these higher affinity. >> That being said, I agree that the rabbit monoclonals have great >> use and even >> more potential. I've used several (many) that are far superior to >> their >> counter-part mouse monoclonals. However, I've used (and heard of) >> a few that >> weren't as good. >> So while I like rabbit monoclonals, use them, advocate for them >> and endorse the >> suggestion you try them, I'm not convinced that as a rule they >> have 10x higher >> affinity than do mouse monoclonals. >> >> Ray Koelling >> Phenopath Laboratories >> Seattle, WA >> >> -------------- Original message ---------------------- >> From: "Mildred Fail" >>> We have had quite a problem with CD3s on bone marrow biopsies being >>> "messy" both with mouse monoclonals and rabbit polyclonals. Protein >>> block is used. Diluting the Ab out further lost some cells in the >>> lymph >>> node control. We tried a rabbit monoclonal. The staining is very >>> specific and intense. The slide is beautifully free of extraneous >>> staining. The higher dilution has not appeared to have effected the >>> number of cells stained. Question is why would the rabbit >>> monoclonal produce a cleaner slide? >>> Rena Fail >>> >>> Rena Fail >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ree3 <@t> leicester.ac.uk Tue Apr 24 06:13:45 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Apr 24 06:14:04 2007 Subject: [Histonet] Lab Week In-Reply-To: <001101c785fd$a8560be0$3601a8c0@brownpathology.net> References: <8C953FEB7AD3A80-1524-42AC@WEBMAIL-RB18.sysops.aol.com> <001101c785fd$a8560be0$3601a8c0@brownpathology.net> Message-ID: In the UK every week is Lab Week, on Monday we work hard, Tuesday very hard, Wednesday extremely hard, Thursday extremely very hard and on Friday, well I'm not too sure what happens, as I have never made it that far!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: 24 April 2007 00:18 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Week Today we had breakfast provided by our executive director Tuesday-- lab provided "gift" handed out to everyone Wednesday--salad day (supervisors provide an assortment of salads) Thursday-- give away day (raffles, etc.) Friday-- pathologists provide lunch Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, April 23, 2007 3:40 PM To: Karen.Heckford@CHW.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week This week we are Monday-------create your own sunday Tuesday------finger food munchies all day Wednesday---lunch from the pathologists Thursday-----death by chocolate day Friday--------covered dish Plus we have a raffle everyday and we do give-aways everyday, small give-aways, but give-aways nontheless. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: Karen.Heckford@CHW.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, 23 Apr 2007 11:23 AM Subject: [Histonet] Lab Week Happy Lab Week Everyone. I hope everyone has a great week. I am just wondering how many labs celebrate everyday. We were told vendors cannot do anything anymore because of some law. We are getting two lunches on Wed. and Friday. Thanks, Karen Heckford HT (ASCP) CE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> CHW.edu Tue Apr 24 07:01:57 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue Apr 24 07:02:03 2007 Subject: [Histonet] Response to Lab Week Message-ID: Okay, I am so jealous of the responses to Lab Week. Wednesday is a Caesar Salad Lunch provided by our Laboratory and Friday a Hot Lunch and raffle provided by the Hospital. Our Pathologists do not do anything. I guess it could be worse and they not celebrate at all. AUGH!!!!! Karen Heckford HT (ASCP) From rjbuesa <@t> yahoo.com Tue Apr 24 07:17:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 24 07:17:49 2007 Subject: [Histonet] tissue processing In-Reply-To: Message-ID: <648217.27829.qm@web61222.mail.yahoo.com> If you are using a tissue processor (TP) you do not have to worry about this problem but since you asked it seems that you are going to process manually. If that is the case you should maintain the same volume ratio of 20:1 as when fixing. The other thing (if processing tissue manually) is that you will not have agitation, pressure, vacuum or controlled temperature, so time has to be increased (as compared with an automatic TP) for each step. Volume alone will not determine proper processing without the other advantages of a TP and these disadvantages have to be compensated with longer steps. Ren? J. mwstarbu@mdanderson.org wrote: Hello, Is there a standard tissue volume to liquid volume ratio that you use when dehydrating samples with ethanol? Thank you in advance. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From doug <@t> ppspath.com Tue Apr 24 08:33:29 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Apr 24 07:32:27 2007 Subject: [Histonet] Response to Lab Week In-Reply-To: Message-ID: Karen, What's worse than salad? :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Tuesday, April 24, 2007 7:02 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Response to Lab Week Okay, I am so jealous of the responses to Lab Week. Wednesday is a Caesar Salad Lunch provided by our Laboratory and Friday a Hot Lunch and raffle provided by the Hospital. Our Pathologists do not do anything. I guess it could be worse and they not celebrate at all. AUGH!!!!! Karen Heckford HT (ASCP) From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 24 07:53:36 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 24 07:53:47 2007 Subject: [Histonet] tissue processing In-Reply-To: <648217.27829.qm@web61222.mail.yahoo.com> Message-ID: Ren? raises an interesting point. While we all accept the 20:1 ratio for fixation as our standard to aim for, I do not believe that this has actually been studied or published as original paper. Please let me know if I am incorrect in this statement. The 20:1 ratio is one of those items that are always passed along. In fact it has been suggested that the concentration of the main fixing agent (within certain limits) and the time of fixation are more important considerations. This principal is used in tanning leather when the amount of glutaraldehyde is carefully matched to the quantity of the proteins in the leather. The aim is to have just the right amount of glutaraldehyde. Excess glutaraldehyde would end up permeating various parts of our anatomy close to the billfold for example. In most labs we only carry out a partial fixation. This is necessitated by time constraints, immunohistochemistry etc. An alternative to immersion fixation for small or thin sample sis vapor fixation. This has the added advantages that no carrier vehicle is necessary, small amount of sometimes expensive fixing agents can be used. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, April 24, 2007 7:18 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue processing If you are using a tissue processor (TP) you do not have to worry about this problem but since you asked it seems that you are going to process manually. If that is the case you should maintain the same volume ratio of 20:1 as when fixing. The other thing (if processing tissue manually) is that you will not have agitation, pressure, vacuum or controlled temperature, so time has to be increased (as compared with an automatic TP) for each step. Volume alone will not determine proper processing without the other advantages of a TP and these disadvantages have to be compensated with longer steps. Ren? J. mwstarbu@mdanderson.org wrote: Hello, Is there a standard tissue volume to liquid volume ratio that you use when dehydrating samples with ethanol? Thank you in advance. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Apr 24 07:58:17 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Apr 24 07:58:27 2007 Subject: [Histonet] Response to Lab Week In-Reply-To: References: Message-ID: Salad cream?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: 24 April 2007 14:33 To: 'Heckford, Karen - SMMC-SF'; 'Histonet' Subject: RE: [Histonet] Response to Lab Week Karen, What's worse than salad? :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Tuesday, April 24, 2007 7:02 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Response to Lab Week Okay, I am so jealous of the responses to Lab Week. Wednesday is a Caesar Salad Lunch provided by our Laboratory and Friday a Hot Lunch and raffle provided by the Hospital. Our Pathologists do not do anything. I guess it could be worse and they not celebrate at all. AUGH!!!!! Karen Heckford HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhkatie <@t> aol.com Tue Apr 24 09:30:31 2007 From: mhkatie <@t> aol.com (mhkatie@aol.com) Date: Tue Apr 24 09:30:53 2007 Subject: [Histonet] Xylene Substitute Message-ID: <8C954943F6EC5BA-D30-7A3F@FWM-R09.sysops.aol.com> Has anyone tried 'Clear -Advantage Xylene Substitute from Polysciences? Did it work as good as they advertise? Thanks, Ann Lynde Medicine Hat Diagnostic Lab Medicine Hat, Alberta ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From CIngles <@t> uwhealth.org Tue Apr 24 10:26:00 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Apr 24 10:26:11 2007 Subject: [Histonet] Response to Lab Week References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A12002F@uwhis-xchng3.uwhis.hosp.wisc.edu> Yea, that would be us with the no celebrations. (outside the lab that is) I guess we get passed over because it is also "Administrative Professionals week". I made lanyards with matching earrings for all my co-workers except for one. (I couldn't figure out what kind would look good on him.;) ). Another coworker of mine makes up certificates with the lab week logo on it. We also put up subtle hints that it is lab week, etc. But we are usually ignored anyway. Anyway... Everyone have a good one and somebody have a chocolate sundae for me this week. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Heckford, Karen - SMMC-SF Sent: Tue 4/24/2007 7:01 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Response to Lab Week Okay, I am so jealous of the responses to Lab Week. Wednesday is a Caesar Salad Lunch provided by our Laboratory and Friday a Hot Lunch and raffle provided by the Hospital. Our Pathologists do not do anything. I guess it could be worse and they not celebrate at all. AUGH!!!!! Karen Heckford HT (ASCP) From sonya.martin <@t> soton.ac.uk Tue Apr 24 10:39:36 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Apr 24 10:40:47 2007 Subject: [Histonet] Aqueous mountant suggestions please Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3572@ISS-CL-EX-V1.soton.ac.uk> Quick query, I need and aqueous mountant for immunohistochemistry slides. I am currently using Vectamount AQ from Vectashield but am having some problems with it not hardening completely and it doesnt seem to last very long ie. the use by date is already up and I'm only halfway through the bottle. Mostly I am mounting immunolabelled frozen tissue sections with DAB. I know aqueous mountants are generally used for AEC but I dont see the point in doing the alcohol and xylene steps if an aqueous mountant works. I dont do paraffin sections so dont already have this set-up. Vector say that their aqueous mountant is also suitable for DAB but is this true for all aqueous mountants? I've been looking at Faramount Aqueous mountant from Dako - any comments? Thanks Sonya From HornHV <@t> archildrens.org Tue Apr 24 11:02:32 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Apr 24 11:02:52 2007 Subject: [Histonet] Aqueous mountant suggestions please In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3572@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7100@EMAIL.archildrens.org> We use Faramount from DAKO. The docs like it. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Tuesday, April 24, 2007 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aqueous mountant suggestions please Quick query, I need and aqueous mountant for immunohistochemistry slides. I am currently using Vectamount AQ from Vectashield but am having some problems with it not hardening completely and it doesnt seem to last very long ie. the use by date is already up and I'm only halfway through the bottle. Mostly I am mounting immunolabelled frozen tissue sections with DAB. I know aqueous mountants are generally used for AEC but I dont see the point in doing the alcohol and xylene steps if an aqueous mountant works. I dont do paraffin sections so dont already have this set-up. Vector say that their aqueous mountant is also suitable for DAB but is this true for all aqueous mountants? I've been looking at Faramount Aqueous mountant from Dako - any comments? Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Erin.Martin <@t> ucsf.edu Tue Apr 24 11:11:16 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Apr 24 11:12:01 2007 Subject: [Histonet] Giemsa as counterstain Message-ID: Hello all, Have any of you every used giemsa as an IHC counterstain for melanin containing tissues? It apparently turns the melanin green which differentiates it from the DAB. My docs have seen this and want to use it here. I bought giemsa solution from American Master Tech and tried it, but the docs said it doesn't look right. If you have used giemsa for this purpose or know what I am doing wrong, I would appreciate any input! Thanks so much, Erin Martin San Francisco From gcallis <@t> montana.edu Tue Apr 24 11:34:28 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 24 11:34:05 2007 Subject: [Histonet] Re: Aqueous mountant suggestions please In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3572@ISS-CL-EX-V1.soto n.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4023E3572@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <6.0.0.22.1.20070424102658.01b11290@gemini.msu.montana.edu> The Faramount works, but it must be heated before coverglass application, and the heating adds another step you have to deal with, Howevere, it is very nice aqueous media that sets up hard and clear. We have also done frozen sections with DAB and set up the dehydration/clearing for permanent mounting media. I must admit this gives a superb final preparation that when dry, the mounting media doesn't retract under the coverglass. It is not that difficult to set up the dehyration/clearing/permanent mountant, and there are some nice mini-staining devices to help you out depending on how many slides you stain at one time. We prefer dehydration/clearing/and permanent media with DAB. At 09:39 AM 4/24/2007, you wrote: >Quick query, > >I need and aqueous mountant for immunohistochemistry slides. I am >currently using Vectamount AQ from Vectashield but am having some >problems with it not hardening completely and it doesnt seem to last >very long ie. the use by date is already up and I'm only halfway through >the bottle. > >Mostly I am mounting immunolabelled frozen tissue sections with DAB. I >know aqueous mountants are generally used for AEC but I dont see the >point in doing the alcohol and xylene steps if an aqueous mountant >works. I dont do paraffin sections so dont already have this set-up. > >Vector say that their aqueous mountant is also suitable for DAB but is >this true for all aqueous mountants? I've been looking at Faramount >Aqueous mountant from Dako - any comments? > >Thanks >Sonya > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Wanda.Smith <@t> HCAhealthcare.com Tue Apr 24 11:42:00 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Apr 24 11:42:05 2007 Subject: [Histonet] Finding Lymph Nodes After Fixation Message-ID: Happy NMLW Everyone!!!!! One of my Pathologists asked me to ask a question: Has anyone had success in finding colonic lymph nodes in fat AFTER fixation with 10% NB Formalin? This Pathologist has tried the Dissect-aid and other solutions that seem to work on fresh fat, but he has had no luck trying this after the fat has been in formalin. Any suggestions or comments would be helpful and appreciated! Thanks, Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com From HornHV <@t> archildrens.org Tue Apr 24 11:43:28 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Apr 24 11:43:38 2007 Subject: [Histonet] Re: Aqueous mountant suggestions please In-Reply-To: <6.0.0.22.1.20070424102658.01b11290@gemini.msu.montana.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7102@EMAIL.archildrens.org> No you do not have to heat Faramount. Use it straight from the bottle. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, April 24, 2007 11:34 AM To: Martin S.; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Aqueous mountant suggestions please The Faramount works, but it must be heated before coverglass application, and the heating adds another step you have to deal with, Howevere, it is very nice aqueous media that sets up hard and clear. We have also done frozen sections with DAB and set up the dehydration/clearing for permanent mounting media. I must admit this gives a superb final preparation that when dry, the mounting media doesn't retract under the coverglass. It is not that difficult to set up the dehyration/clearing/permanent mountant, and there are some nice mini-staining devices to help you out depending on how many slides you stain at one time. We prefer dehydration/clearing/and permanent media with DAB. At 09:39 AM 4/24/2007, you wrote: >Quick query, > >I need and aqueous mountant for immunohistochemistry slides. I am >currently using Vectamount AQ from Vectashield but am having some >problems with it not hardening completely and it doesnt seem to last >very long ie. the use by date is already up and I'm only halfway through >the bottle. > >Mostly I am mounting immunolabelled frozen tissue sections with DAB. I >know aqueous mountants are generally used for AEC but I dont see the >point in doing the alcohol and xylene steps if an aqueous mountant >works. I dont do paraffin sections so dont already have this set-up. > >Vector say that their aqueous mountant is also suitable for DAB but is >this true for all aqueous mountants? I've been looking at Faramount >Aqueous mountant from Dako - any comments? > >Thanks >Sonya > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From opiecurt <@t> yahoo.com Tue Apr 24 11:47:35 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Tue Apr 24 11:47:41 2007 Subject: [Histonet] how to keep toenail on the slide Message-ID: <546697.59915.qm@web81612.mail.mud.yahoo.com> i keep having my sections float off, not all but most. any suggestions? --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From burch007 <@t> mc.duke.edu Tue Apr 24 12:33:07 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Tue Apr 24 12:33:14 2007 Subject: [Histonet] Giemsa as counterstain In-Reply-To: Message-ID: Erin - here is the protocol I use for staining melanin with Giemsa. Post DAB Giemsa Counterstain for Melanin Staining From DAB, wash slides in tap water. Counterstain with hematoxylin, wash, ?blue, and wash per usual technique. Rinse with DI water. Place slides in full strength May Grunewald solution for one minute. Pour off MG solution and drain excess from slides. Pour on working Giemsa solution for two minutes. Gently rinse slides in tap water for 1 minute. Differentiation Place slides in 95% ETOH for 2 minutes. ** Transfer slides to 95% ETOH with colophonium resin for 2 minutes. ** Transfer slides to a second 95% ETOH with colophonium resin for 2 minutes. ** Dehydrate and clear slides, mount with permanent mounting media. **gently agitate or dip slides to facilitate removal of excess stain** Giemsa working solution: 2.5 ml Giemsa stock in 50 ml DI water Colophonium working solution: 10 drops 10% colophonium solution in 100 ml 95% ETOH Giemsa, Sigma GS-1L May-Gruenwald, Sigma MG1-L Note: Differentiation can be accomplished with a 0.1-0.2% acetic acid solution. This will be very rapid in comparison to the 95% ETOH with colophonium. It will also remove the hematoxylin. A hematoxylin counterstain is not necessary, but does make a nice combination with the Giemsa counter stain. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Martin, Erin" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/24/2007 11:11 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Giemsa as counterstain Hello all, Have any of you every used giemsa as an IHC counterstain for melanin containing tissues? It apparently turns the melanin green which differentiates it from the DAB. My docs have seen this and want to use it here. I bought giemsa solution from American Master Tech and tried it, but the docs said it doesn't look right. If you have used giemsa for this purpose or know what I am doing wrong, I would appreciate any input! Thanks so much, Erin Martin San Francisco _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Tue Apr 24 12:54:54 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Tue Apr 24 12:55:10 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <200704241301.18d462e37d11ef@rly-yb03.mx.aol.com> References: <200704241301.18d462e37d11ef@rly-yb03.mx.aol.com> Message-ID: <8C954B0CCB71184-138C-3D3A@FWM-M43.sysops.aol.com> Wanda Smith asks: >>One of my pathologists asked me to ask a question - Has anyone had success in finding colonic lymph nodes in fat AFTER fixation with 10% neutral buffered formalin? This pathologist has tried Dissect-aid and other solutions that seem to work on fresh fat, but he has had no luck trying this after the fat has been in formalin.<< No, this doesn't work. Though often when the specimen arrives in formalin the fat has been so little penetrated by formalin that using a disclosing fixative is still worthwhile. Any other specialty, you'd be able to make arrangements with the operating room staff to solve this problem. In pathology, the red-haired stepchild again. And the clinical stakes are high here - one positive lymph node, however small, requires chemotherapy. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From gcallis <@t> montana.edu Tue Apr 24 13:04:50 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Apr 24 13:04:29 2007 Subject: [Histonet] I stand corrected on heating Faramount, it was Glycergel we heated In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7102@EMAIL.archildrens .org> References: <6.0.0.22.1.20070424102658.01b11290@gemini.msu.montana.edu> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7102@EMAIL.archildrens.org> Message-ID: <6.0.0.22.1.20070424120055.01b133c8@gemini.msu.montana.edu> I stand corrected, it was DAKO's Glycergel mounting media we heated in Chris van der Loos lab - and it set up hard after drying. Thanks for the heads up, Hazel. 10:43 AM 4/24/2007, you wrote: >No you do not have to heat Faramount. Use it straight from the bottle. > >Hazel Horn Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From anh2006 <@t> med.cornell.edu Tue Apr 24 13:08:05 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Apr 24 13:08:20 2007 Subject: [Histonet] Re: Aqueous mountant suggestions please In-Reply-To: <6.0.0.22.1.20070424102658.01b11290@gemini.msu.montana.edu> References: <71437982F5B13A4D9A5B2669BDB89EE4023E3572@ISS-CL-EX-V1.soton.ac.uk> <6.0.0.22.1.20070424102658.01b11290@gemini.msu.montana.edu> Message-ID: We don't heat Faramount and it works well for our chromagens such as AEC+. And like Gayle's lab, for DAB+ frozens we dehydrate and clear and get beautiful, permanent results. Highly recommended. Andrea At 10:34 AM -0600 4/24/07, Gayle Callis wrote: >The Faramount works, but it must be heated before coverglass >application, and the heating adds another step you have to deal >with, Howevere, it is very nice aqueous media that sets up hard and >clear. > >We have also done frozen sections with DAB and set up the >dehydration/clearing for permanent mounting media. I must admit >this gives a superb final preparation that when dry, the mounting >media doesn't retract under the coverglass. It is not that >difficult to set up the dehyration/clearing/permanent mountant, and >there are some nice mini-staining devices to help you out depending >on how many slides you stain at one time. We prefer >dehydration/clearing/and permanent media with DAB. > > > >At 09:39 AM 4/24/2007, you wrote: >>Quick query, >> >>I need and aqueous mountant for immunohistochemistry slides. I am >>currently using Vectamount AQ from Vectashield but am having some >>problems with it not hardening completely and it doesnt seem to last >>very long ie. the use by date is already up and I'm only halfway through >>the bottle. >> >>Mostly I am mounting immunolabelled frozen tissue sections with DAB. I >>know aqueous mountants are generally used for AEC but I dont see the >>point in doing the alcohol and xylene steps if an aqueous mountant >>works. I dont do paraffin sections so dont already have this set-up. >> >>Vector say that their aqueous mountant is also suitable for DAB but is >>this true for all aqueous mountants? I've been looking at Faramount >>Aqueous mountant from Dako - any comments? >> >>Thanks >>Sonya >> -- From Heather.D.Renko <@t> osfhealthcare.org Tue Apr 24 13:15:43 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Apr 24 13:15:18 2007 Subject: [Histonet] Aqueous mountant Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775473@pmc-rfd-mx01.intranet.osfnet.org> I have used the Fara mount Aqueous mountant from Dako and was very pleased with the results. Dried nicely. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Heather.D.Renko <@t> osfhealthcare.org Tue Apr 24 13:17:41 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Apr 24 13:17:14 2007 Subject: [Histonet] Faramount Aqueous mountant from Dako Message-ID: <40026EDDE64CDA47AB382C52619ACD3C07775474@pmc-rfd-mx01.intranet.osfnet.org> Additionally, I warmed my in the waterbath before using-very nice and clean. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Jason.Wiese <@t> va.gov Tue Apr 24 13:38:47 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Apr 24 13:39:53 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <8C954B0CCB71184-138C-3D3A@FWM-M43.sysops.aol.com> References: <200704241301.18d462e37d11ef@rly-yb03.mx.aol.com> <8C954B0CCB71184-138C-3D3A@FWM-M43.sysops.aol.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E1296D@VHAV20MSGA3.v20.med.va.gov> I disagree. I use Presto-Fixe II from spectra-tint for fat and lymph node. Even after formalin fixation, you can throw your pericolonic fat, etc.. in it, and it will turn the lymph nodes white... Jason Wiese, BS, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Tuesday, April 24, 2007 10:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Finding Lymph Nodes After Fixation Wanda Smith asks: >>One of my pathologists asked me to ask a question - Has anyone had success in finding colonic lymph nodes in fat AFTER fixation with 10% neutral buffered formalin? This pathologist has tried Dissect-aid and other solutions that seem to work on fresh fat, but he has had no luck trying this after the fat has been in formalin.<< No, this doesn't work. Though often when the specimen arrives in formalin the fat has been so little penetrated by formalin that using a disclosing fixative is still worthwhile. Any other specialty, you'd be able to make arrangements with the operating room staff to solve this problem. In pathology, the red-haired stepchild again. And the clinical stakes are high here - one positive lymph node, however small, requires chemotherapy. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From splaza <@t> tylerpathology.com Tue Apr 24 13:52:50 2007 From: splaza <@t> tylerpathology.com (Susan Plaza) Date: Tue Apr 24 13:52:55 2007 Subject: [Histonet] RE: Giemsa as counterstain Message-ID: <000501c786a1$c2aaab50$800aa8c0@domain.local> I did the same thing (using the Giemsa solution from American Master Tech). Apparently, you should do the entire Giemsa Stain (Working Jenner Solution followed by Working Giemsa Solution and differentiated in 1% Aqueous Acetic Acid. Unfortunately, I did not do this and my pathologist didn't want me to give it another shot. Let me know if it works. Susan Plaza, HT(ASCP), QIHC From TJJ <@t> Stowers-Institute.org Tue Apr 24 13:54:44 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Apr 24 13:55:09 2007 Subject: [Histonet] Re: Two questions about double IHC Message-ID: Amy, you asked: >snip< I need to do double IHC on FFPE mouse tissue. Unluckily two antibodies I have to use are all rat anti-mouse. The protocol for these antibodies are very similar: Pro K digestion, Primary antibody, Rb anti-Rat 2ND antibody and the rest is following PerkinElmer TSA biotin system instruction (Streptavidin-HRP, biotinylated-tyramide and Streptavidin-HRP). I will perform the two IHC sequentially. I have two questions: 1. Do I need to do Proteinase K for the second antigen? 2. Can I keep all other steps the same and just use Streptavidin-FITC or Streptavidin-Texas Red at the final step to differentiate two antigen? BTW, could you recommend a good website to learn double IHC? >snip< You cannot do the double staining as you describe. By using the same detection method for both, you will end up labeling both antibodies with both colors. If you try eluting the first antigen-antibody complex after labeling with the first antibody, you will lose your first label and will only show labeling of the second. (does this make sense?) It's best if you have two different animal species, or use two different methods for detection. Having both from the same species AND having both require tyramide amplification makes your task next to impossible by fluorescence. It may be possible to do this chromogenically using DAB in a sequential staining protocol, but it's going to be complicated. It's even more complex if you think you will have co-localization. I'll take a stab at it putting something together that might work, but I'm hoping Chris van der Loos (if he's on list) or Gayle Callis comes up with something easier: Primary antibody #1 labeling: Pro K digestion, rat anti-mouse primary Ab 1, biotinylated Rabbit anti-rat, Streptavidin-HRP, biotinylated-tyramide, Streptavidin-HRP, DAB. Elute using heat-induced antigen retrieval (shouldn't need PK digestion at this point) Rat anti-mouse secondary Ab 2, HRP-labeled anti-rat antibody, FITC-tyramide, anti-FITC AP, NBT/BCIP or other stable chromogen Nuclear fast red counterstain (If the above chromogens used). Co-localization will be impossible to see with this color combination. You will need adequate controls doing this experiment. That could potentially be a lot of slides with one experiment. It might be easier to try to optimize one of your antibodies with a higher concentration and no tyramide. Have you tried not using tyramide? Sometimes using polymer HRP systems work well. Look for anti-rat polymer, I think Biocare Medical might have some? Or replace one of the antibodies with one made in a different species (should be much easier), and then try to optimize it without tyramide. Or use labeled antibodies, one with biotin, one with FITC, and use two different detections. You're going to have to simplify this before you can really get good results that you can trust. Best reference I know is Chris van der Loos' book on Multiple Immunostaining, ISBN 1-85996-187-8. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Valerie.Hannen <@t> parrishmed.com Tue Apr 24 14:24:09 2007 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Apr 24 14:24:15 2007 Subject: [Histonet] Colonic Lymph Nodes Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34AB@ISMAIL.parrishmed.local> Happy Lab Week to all, I am responding to Wanda's request for suggestions for finding colonic nodes after fixation. While we do not use 10% NBF(we use Zinc Formalin), we do use a product after fixation. This is solution is called "O-FIX" and we buy it from Surgipath. It does a wonderul job of enhancing the nodes so that they can be easily found. If enhancing is not time critical, we place the peri-colonic / mesentaric fat in the "O-FIX" overnight. If time is critical, we place the fat in the "O-FIX" and heat it at 42-43 degrees centigrade for approximately 3 hours. I hope this will help. Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida 32796 (321) 268-6333 Ext. 7506 ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From safety.trainorlab <@t> mts.net Tue Apr 24 14:38:12 2007 From: safety.trainorlab <@t> mts.net (SAFETY TRAINORLAB) Date: Tue Apr 24 14:38:01 2007 Subject: [Histonet] overstained Fontana-Masson Message-ID: <000801c786a8$18d52cc0$19c809c0@tlabs> Fontana for melanin was done on a very tiny skin biopsy and was overstained with silver nitrate. There is no specimen left in the block to recut. Does anybody have a method to remove the silver so that it can be restained? Thanks. Joyce Winnipeg Canada From Valerie.Hannen <@t> parrishmed.com Tue Apr 24 14:50:42 2007 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Apr 24 14:50:50 2007 Subject: [Histonet] Lab Week Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34AC@ISMAIL.parrishmed.local> Boy do we celebrate... Monday: breakfast provided by lab folks Tuesday: BBQ lunch provided by the pathologists Wednesday: Pizza lunch provided by the Lab Director Thursday: we have a "cooking" Contest...this year is 5 ingredients or less recipes...1st place gets a $30 gift certicate to a local store. Friday: catered Lunch by our Cafeteria All week we play various games put together by various lab folks ( we only play at break, lunch and take them home). There are 1st, 2nd and 3rd place prizes for each game. Every Lab employee also gets a "door prize" just for working here. We really celebrate our selves and our accomplishments and we also vote on a Lab Employee of the Year. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida 32796 ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 24 14:56:13 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Apr 24 14:56:18 2007 Subject: [Histonet] Hiring PA Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640BF3@hpes1.HealthPartners.int> My manager is out on maternity leave and I am in need of hiring a PA or PA tech. Would anyone out there be willing to share an ad and/or job description for a histotech that does grossing, not processing? I would appreciate any help in this area now that the newest hat I wear is interim manager along with technical supervisor and bench tech!!! Time is a commodity I am out of so any way I can make things a little easier for myself right now is a bonus to me!!!!!! Thank you ahead of time!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Tracey.Lenek <@t> CLS.ab.ca Tue Apr 24 15:13:44 2007 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Tue Apr 24 15:14:48 2007 Subject: [Histonet] AFB Contamination Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From JWEEMS <@t> sjha.org Tue Apr 24 15:18:44 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 24 15:19:12 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <8C954B0CCB71184-138C-3D3A@FWM-M43.sysops.aol.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF95F3@sjhaexc02.sjha.org> We use Clark's fixative = Carnoy's without chloroform. It works well even after the specimen has been in formalin. Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Tuesday, April 24, 2007 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Finding Lymph Nodes After Fixation Wanda Smith asks: >>One of my pathologists asked me to ask a question - Has anyone had success in finding colonic lymph nodes in fat AFTER fixation with 10% neutral buffered formalin? This pathologist has tried Dissect-aid and other solutions that seem to work on fresh fat, but he has had no luck trying this after the fat has been in formalin.<< No, this doesn't work. Though often when the specimen arrives in formalin the fat has been so little penetrated by formalin that using a disclosing fixative is still worthwhile. Any other specialty, you'd be able to make arrangements with the operating room staff to solve this problem. In pathology, the red-haired stepchild again. And the clinical stakes are high here - one positive lymph node, however small, requires chemotherapy. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bhewlett <@t> cogeco.ca Tue Apr 24 15:19:39 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Apr 24 15:19:43 2007 Subject: [Histonet] AFB Contamination References: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> Message-ID: <002c01c786ad$e2fffd40$6500a8c0@mainbox> Tracey, Contaminated waterbaths, tap water rinses and re-used staining solutions have all been found to be a potential source of contamination. I would suspect tap nozzles as the #1 source. Bryan ----- Original Message ----- From: To: Sent: Tuesday, April 24, 2007 4:13 PM Subject: [Histonet] AFB Contamination Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Apr 24 15:19:10 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Apr 24 15:20:32 2007 Subject: [Histonet] Lab Week Photo Message-ID: <000901c786ad$d2a76b40$fa052e4b@HPPav2> If you want a photo and information on histotechnology and Lab Week, to post on your lab's bulletin board or to hand out to people, the NSH website has one. Looks great in color, prints out well in black and white. http://www.nsh.org/PDF/LabWeek2007.pdf Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From liz <@t> premierlab.com Tue Apr 24 15:27:22 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Apr 24 15:27:35 2007 Subject: [Histonet] AFB Contamination In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> Message-ID: <000901c786ae$f6ee43b0$0d00a8c0@domain.Premier> Tracey There is a ton of mycobacterium in tap water and in the plumbing system. We always use filtered water in our water bath for AFB staining. Check out Freidas book and staining for AFB. The culprit is probably your waterbath, but it could also be your AFB stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Tuesday, April 24, 2007 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Contamination Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From lpwenk <@t> sbcglobal.net Tue Apr 24 15:27:16 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Apr 24 15:28:37 2007 Subject: [Histonet] AFB Contamination In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> Message-ID: <000a01c786ae$f3bed5b0$fa052e4b@HPPav2> Send samples of water (tap, d. water, flotation bath, etc) to the microbiology lab, and have them do cultures on it. These are a possible source of bacteria. Some parts of the US have non-pathogenic acid fast bacteria in their water supply (OK to drink, but a concern in labs for false positives). In our lab (Michigan), a couple of years after we moved into a new lab area, we found gram negative water in the d. water. They had hooked up the d. water lines wrong, and it took us months before they were able to eliminate all the bacteria. Also, what is your control slide? Any chance it's a smear? Bacteria could be wafting off from that. Other people on Histonet have also commented that they have experienced the same phenomena of bacteria coming off the fixed, processed control slide, when the control is loaded with AFB. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Tuesday, April 24, 2007 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Contamination Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Tue Apr 24 15:39:22 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Apr 24 15:39:44 2007 Subject: [Histonet] AFB Contamination In-Reply-To: <002c01c786ad$e2fffd40$6500a8c0@mainbox> References: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> <002c01c786ad$e2fffd40$6500a8c0@mainbox> Message-ID: <6.2.5.6.2.20070424163334.01fc4940@vet.upenn.edu> Tracey, If you are using a deionized water system you may want to check when you filters were last changed. Many times the filters are not changed for laboratories until someone calls and reports they are out of date or have a problem. Biofilm and bacteria often grow in the pieces of tubing we attach to the nozzles on sinks to keep the water from coming out to strongly. They should be changed often to assure they are not a problem also. I have not ever used tap water in my water bath or for any making up any of my solutions. We even use deionized or distilled for all rinses if a bacterial stain is required. Just saves a few more moments of my sanity. Pam Marcum At 04:19 PM 4/24/2007, Bryan Hewlett wrote: >Tracey, > >Contaminated waterbaths, tap water rinses and re-used staining >solutions have all been found to be a potential source of contamination. >I would suspect tap nozzles as the #1 source. > >Bryan > >----- Original Message ----- From: >To: >Sent: Tuesday, April 24, 2007 4:13 PM >Subject: [Histonet] AFB Contamination > > >Hi, > >We are experiencing transient false positive ZN staining on skin >biopies. Today a slide had clumped AFB over the majority of the >slide where no tissue was present. Previous to this, only one or >two bacilli were noted on the slide. >Has anyone investigated and found a contaminated waterbath or >perhaps the Kinyoun's is the culprit as our lab reuses both? Any >help would be appreciated. > >Tracey > > >CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may >contain confidential, personal and/or privileged information >intended for a specific purpose and recipient. If you are not the >intended recipient do not disclose, copy, retain, distribute, use or >modify any of the contents of this transmission. If you received >this transmission in error please notify me immediately by return >e-mail or telephone and destroy the entire transmission and any >copies produced. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From lpwenk <@t> sbcglobal.net Tue Apr 24 15:42:25 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Apr 24 15:43:47 2007 Subject: [Histonet] overstained Fontana-Masson In-Reply-To: <000801c786a8$18d52cc0$19c809c0@tlabs> Message-ID: <000b01c786b1$11992110$fa052e4b@HPPav2> Below is from a 2002 Histonet response from Robert Lott about removing silver from an overstained GMS, that I saved in my "good to know in case I need it" file. - - - - Below is a treatment that really works, but it is best to go really slow and check microscopically over and over until the desired result is obtained. 1. De-coverslip sections and run then back to distilled water. 2. Place section(s) in coplin jar containing 1 part of 1% potassium "ferri"cyanide to 3 parts distilled water (0.25% potassium ferricyanide) 3. Monitor continuously with the microscope until excess silver has been removed. 4. Wash well in distilled water. 5. Re-counterstain as dersired. - - - - Note from me: Make certain it is potassium ferrIcyanide (red crystals, such as used in the Schmorl stain), NOT potassium ferrOcyanide (yellow flakes, used in the Prussian blue iron stain). Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SAFETY TRAINORLAB Sent: Tuesday, April 24, 2007 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overstained Fontana-Masson Fontana for melanin was done on a very tiny skin biopsy and was overstained with silver nitrate. There is no specimen left in the block to recut. Does anybody have a method to remove the silver so that it can be restained? Thanks. Joyce Winnipeg Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hancockestates <@t> yahoo.com Tue Apr 24 15:50:25 2007 From: hancockestates <@t> yahoo.com (MaryAnn Hancock) Date: Tue Apr 24 15:50:29 2007 Subject: [Histonet] AUTOMATED BLOCK AND SLIDE STORAGE Message-ID: <890197.29844.qm@web51611.mail.re2.yahoo.com> Does anyone know of an automated or semi automated system for the storage of blocks and slides? Thanks MaryAnn --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From hancockestates <@t> yahoo.com Tue Apr 24 15:53:41 2007 From: hancockestates <@t> yahoo.com (MaryAnn Hancock) Date: Tue Apr 24 15:53:46 2007 Subject: [Histonet] BIOPSY PROGRAM Message-ID: <20070424205341.28471.qmail@web51603.mail.re2.yahoo.com> I am interested in Short Biopsy Programs on the VIP and Leica TP 1050. Thanks MA --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Tue Apr 24 16:01:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 24 16:01:28 2007 Subject: [Histonet] AFB Contamination In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> Message-ID: <560534.37911.qm@web61225.mail.yahoo.com> There is a good wealth of references on this issue in Histonet archieves. It has been pointed out several times the AFB+ organisms can develop in water pipes and several containers. Programmed cleaning of all water container can eliminate this problem. Ren? J. Tracey.Lenek@CLS.ab.ca wrote: Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From laurie.colbert <@t> huntingtonhospital.com Tue Apr 24 16:17:16 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Apr 24 16:17:22 2007 Subject: [Histonet] AFB Contamination Message-ID: <57BE698966D5C54EAE8612E8941D768301268CDA@EXCHANGE3.huntingtonhospital.com> We recently had this same problem and traced it to the water containers on our automated stainer. I bleached them out really well, but the bacteria were still there, so now we run all specials for microorganisms down by hand in our special stains area. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Tuesday, April 24, 2007 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Contamination Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MBURTON1 <@t> PARTNERS.ORG Tue Apr 24 16:36:23 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Tue Apr 24 16:35:03 2007 Subject: [Histonet] Newborn mouse brain Message-ID: Does anyone have suggestions on how to fix and process newborn mouse heads/brain? What is the best way to adequately fix and process without perfusion or removal of the brain first? We would like to stain brain sections with immunofluorescence and possibly IHC. Thank you. Mark Burton HTL (ASCP) Molecular Aging & Development Lab Brigham & Women's Hospital Boston, MA The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From SCOTT.TURNER <@t> SPCORP.COM Tue Apr 24 18:11:34 2007 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Tue Apr 24 18:11:38 2007 Subject: [Histonet] Re: Two questions about double IHC In-Reply-To: Message-ID: <9A919A5D70313A4D9C56A025710874082907A0@kenmsg40.us.schp.com> I think there might be a way to do this. The tyramide doesn't actually bind to the immune complex but rather precipitates and covalently binds to the tyrosine residues in the tissue proteins. Therefore, I think you could use the non-biotin fluorescyl-tyramide kit to stain your first antibody, then elute the immune complex leaving the fluorescyl-tyramide behind. Then you'd have to get a little tricky. Since both of your antibodies are rat anti-mouse, you'll have to pre-label your other primary somehow. The easiest thing to do would be to biotinylate that primary. Alternatively, you could pre-complex your biotinylated secondary Ab with your second primary and block the unbound secondary Ab with mouse serum so that it doesn't stick to your first primary antibody. You can figure out the concentrations that work by yourself or you could use the biotinylation and blocking reagents from the Dako ARK. After you've done that, you can put the primary/secondary complex on the tissue and follow with your biotin-based TSA and a Streptavidin-Texas red. Anyway, that's what I could come up with. I know it sounds really complicated but I think it might work (with a LOT of trial and error to get it right). Does anyone out there have any input on this? Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Tuesday, April 24, 2007 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two questions about double IHC Amy, you asked: >snip< I need to do double IHC on FFPE mouse tissue. Unluckily two antibodies I have to use are all rat anti-mouse. The protocol for these antibodies are very similar: Pro K digestion, Primary antibody, Rb anti-Rat 2ND antibody and the rest is following PerkinElmer TSA biotin system instruction (Streptavidin-HRP, biotinylated-tyramide and Streptavidin-HRP). I will perform the two IHC sequentially. I have two questions: 1. Do I need to do Proteinase K for the second antigen? 2. Can I keep all other steps the same and just use Streptavidin-FITC or Streptavidin-Texas Red at the final step to differentiate two antigen? BTW, could you recommend a good website to learn double IHC? >snip< You cannot do the double staining as you describe. By using the same detection method for both, you will end up labeling both antibodies with both colors. If you try eluting the first antigen-antibody complex after labeling with the first antibody, you will lose your first label and will only show labeling of the second. (does this make sense?) It's best if you have two different animal species, or use two different methods for detection. Having both from the same species AND having both require tyramide amplification makes your task next to impossible by fluorescence. It may be possible to do this chromogenically using DAB in a sequential staining protocol, but it's going to be complicated. It's even more complex if you think you will have co-localization. I'll take a stab at it putting something together that might work, but I'm hoping Chris van der Loos (if he's on list) or Gayle Callis comes up with something easier: Primary antibody #1 labeling: Pro K digestion, rat anti-mouse primary Ab 1, biotinylated Rabbit anti-rat, Streptavidin-HRP, biotinylated-tyramide, Streptavidin-HRP, DAB. Elute using heat-induced antigen retrieval (shouldn't need PK digestion at this point) Rat anti-mouse secondary Ab 2, HRP-labeled anti-rat antibody, FITC-tyramide, anti-FITC AP, NBT/BCIP or other stable chromogen Nuclear fast red counterstain (If the above chromogens used). Co-localization will be impossible to see with this color combination. You will need adequate controls doing this experiment. That could potentially be a lot of slides with one experiment. It might be easier to try to optimize one of your antibodies with a higher concentration and no tyramide. Have you tried not using tyramide? Sometimes using polymer HRP systems work well. Look for anti-rat polymer, I think Biocare Medical might have some? Or replace one of the antibodies with one made in a different species (should be much easier), and then try to optimize it without tyramide. Or use labeled antibodies, one with biotin, one with FITC, and use two different detections. You're going to have to simplify this before you can really get good results that you can trust. Best reference I know is Chris van der Loos' book on Multiple Immunostaining, ISBN 1-85996-187-8. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From jnocito <@t> satx.rr.com Tue Apr 24 18:37:42 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Apr 24 18:37:43 2007 Subject: [Histonet] how to keep toenail on the slide References: <546697.59915.qm@web81612.mail.mud.yahoo.com> Message-ID: <00fc01c786c9$8df09050$d49eae18@yourxhtr8hvc4p> Joe the Toe here. I believe I can ass ist you here. (oops, I know I just mad someone mad, but, it wouldn't be me if I didn't). Have you tried positive charged slides? Do you soak your nails in a solution before cutting? We soak our nails in 20% sodium hydroxide (thank you Rene), then cut them and place on Plus Slides. We place the slides upright in an 80 C oven for 15 minutes and let the slides cool completely before staining. Need to make sure that all the water is out. Hope this helps. JTT ----- Original Message ----- From: "curt tague" To: Sent: Tuesday, April 24, 2007 11:47 AM Subject: [Histonet] how to keep toenail on the slide >i keep having my sections float off, not all but most. any suggestions? > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Tue Apr 24 18:46:16 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Tue Apr 24 18:46:31 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <200704241718.1df462e741079@rly-yc05.mail.aol.com> References: <200704241718.1df462e741079@rly-yc05.mail.aol.com> Message-ID: <8C954E1E2A40EF2-138C-5752@FWM-M43.sysops.aol.com> Well, three different people have claimed they can get away with post-fixing colonic mesenteries in a disclosing fixative to look for lymph nodes. I'm surprised - theoretically it shouldn't work, in my personal experience it doesn't work, and several people have told me that they couldn't make it work - so I felt justified in pontificating. Well, I'll try it again if the opportunity arises. I wonder if the mesenteries received in formalin were adequately fixed - it's a situation where formalin penetrates very slowly. My personal preference is Davidson's fixative (3 parts water, 3 parts alcohol, 2 parts 37% formaldehyde solution, 1 part glacial acetic acid). Surgipath's O-Fix in my experience works quite as well. I've had no luck Googling "Presto-Fixe II from spectra-tint" - look at the MSDS and tell us what's in it. Once again I want to emphasize the clinical seriousness of this problem - lymph node metastases from colon cancer are often the size of pinheads, very hard to find - and their presence determines treatment. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Jason.Wiese <@t> va.gov Tue Apr 24 19:03:28 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Apr 24 19:03:37 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <8C954E1E2A40EF2-138C-5752@FWM-M43.sysops.aol.com> References: <200704241718.1df462e741079@rly-yc05.mail.aol.com> <8C954E1E2A40EF2-138C-5752@FWM-M43.sysops.aol.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12970@VHAV20MSGA3.v20.med.va.gov> Presto-fixe II Active ingredients: 1.0 Molar Formaldehyde 0.2 Molar Glyoxal 60% Methanol Buffered plus PVP(macromolecular colloidal membrane protectant) That's it... I agree with you 100% on the clinical seriousness of this problem. I am in no way saying that presto-fixe II will solve this problem, nor am I endorsing the product as some great mystery which has been overlooked. I only know that, at times, I have place mesentery that has been sitting in formalin for a long time (days) in this product, and I have noticed the nodes turn white and firm up a bit. Thanks, Jason -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Tuesday, April 24, 2007 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Finding Lymph Nodes After Fixation Well, three different people have claimed they can get away with post-fixing colonic mesenteries in a disclosing fixative to look for lymph nodes. I'm surprised - theoretically it shouldn't work, in my personal experience it doesn't work, and several people have told me that they couldn't make it work - so I felt justified in pontificating. Well, I'll try it again if the opportunity arises. I wonder if the mesenteries received in formalin were adequately fixed - it's a situation where formalin penetrates very slowly. My personal preference is Davidson's fixative (3 parts water, 3 parts alcohol, 2 parts 37% formaldehyde solution, 1 part glacial acetic acid). Surgipath's O-Fix in my experience works quite as well. I've had no luck Googling "Presto-Fixe II from spectra-tint" - look at the MSDS and tell us what's in it. Once again I want to emphasize the clinical seriousness of this problem - lymph node metastases from colon cancer are often the size of pinheads, very hard to find - and their presence determines treatment. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anthony <@t> histotechexchange.com Tue Apr 24 23:53:33 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Tue Apr 24 23:54:02 2007 Subject: [Histonet] Temporary Histologist Position in Hays KS Message-ID: <3216.71.51.6.248.1177476836.squirrel@host4.wfdns.com> I have a great temporary job going for 13 weeks in Hays KS. Grossing is a large part of the job and previous experience is needed. The position starts 06/06/07. There is overtime almost every week and we pay it at double time. If you have any intrest please contact us. Yours truly, Anthony Williams Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@Histotechexchange.com www.histotechexchange.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 25 01:36:31 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 25 01:36:36 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012475DD@wahtntex2.waht.swest.nhs.uk> Terry will probably remember sticking mesentery in methyl salycylate (is that how you spell it?) to reveal lymph nodes but I honestly can't remember if it worked at all. I know it had a funny smell and smelled of old people. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The great question... which I have not been able to answer... is, "What does a woman want? Sigmund Freud This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sonya.martin <@t> soton.ac.uk Wed Apr 25 07:19:24 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Apr 25 07:19:43 2007 Subject: [Histonet] An histology puzzle? Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> I have an image of an H&E stained structure that I want to submit to a micrograph competition (it is to be judged on artistic rather than scientific merit!). Does anyone know what it might be? It was in an FFPE mouse kidney section - but not within the kidney tissue. Is it a stucture or an artifact? Please go to www.flickr.com/photos/sonzer to see it. Sonya ;-) From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 25 07:24:05 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 25 07:24:16 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation Message-ID: <407F05A128805F4C879A33DBA32E618E20C2D0@TRFT-EX01.xRothGen.nhs.uk> Yes Kem, I remember it well. It worked fairly well, but what a load of trouble it was. Tissue went rather light mahogany and stiff, but the fat was cleared. BTW, it's old people who smell of methyl salicylate rather than the other way round. It's all the rubs for their painful joints (oil of Wintergreen). Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 25 April 2007 07:37 To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Finding Lymph Nodes After Fixation Terry will probably remember sticking mesentery in methyl salycylate (is that how you spell it?) to reveal lymph nodes but I honestly can't remember if it worked at all. I know it had a funny smell and smelled of old people. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The great question... which I have not been able to answer... is, "What does a woman want? Sigmund Freud This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Wed Apr 25 07:30:16 2007 From: bhewlett <@t> cogeco.ca (bhewlett@cogeco.ca) Date: Wed Apr 25 07:30:20 2007 Subject: [Histonet] An histology puzzle? Message-ID: <462f49d8.31b.9e0.16306@cogeco.ca> > Sonja, The photo is of an artery cut on an angle through a bend! Bryan > I have an image of an H&E stained structure that I want to submit to a > micrograph competition (it is to be judged on artistic rather than > scientific merit!). Does anyone know what it might be? > > It was in an FFPE mouse kidney section - but not within the kidney > tissue. > > Is it a stucture or an artifact? > > Please go to www.flickr.com/photos/sonzer to see it. > > > Sonya ;-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Wed Apr 25 07:31:41 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Apr 25 07:31:50 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: Looks to me like a tangential section near the edge of a glomerulus (a chord through a sphere kind of section). Hm ... this sort of thing would be good for Fridays. Phil >I have an image of an H&E stained structure that I want to submit to a >micrograph competition (it is to be judged on artistic rather than >scientific merit!). Does anyone know what it might be? > >It was in an FFPE mouse kidney section - but not within the kidney >tissue. > >Is it a stucture or an artifact? > >Please go to www.flickr.com/photos/sonzer to see it. > > >Sonya ;-) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 25 07:32:09 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 25 07:32:13 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012475EB@wahtntex2.waht.swest.nhs.uk> BTW, it's old people who smell of methyl salicylate rather than the other way round. It's all the rubs for their painful joints (oil of Wintergreen). Terry You using it too? Still trying cod liver oil and glucosamine to repair my karate destroyed joints. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From oshel1pe <@t> cmich.edu Wed Apr 25 07:35:12 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Apr 25 07:35:20 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2D0@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E20C2D0@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Me salicylate is great for clearing small vertebrates for Alizarin Red-S/Alcian Blue skeletal stains. Even shows the air bladders of fish for a few days (before the Me salicylate diffuses across the bladder wall). Doubt it helped their joints any, though. Phil ----- Wonko the Sane was right. >Yes Kem, I remember it well. It worked fairly well, but what a load of >trouble it was. Tissue went rather light mahogany and stiff, but the fat >was cleared. > >BTW, it's old people who smell of methyl salicylate rather than the >other way round. It's all the rubs for their painful joints (oil of >Wintergreen). > >Terry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo >Rogerson >Sent: 25 April 2007 07:37 >To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Re: Finding Lymph Nodes After Fixation > >Terry will probably remember sticking mesentery in methyl salycylate (is >that how you spell it?) to reveal lymph nodes but I honestly can't >remember if it worked at all. > >I know it had a funny smell and smelled of old people. > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; > >The great question... which I have not been able to answer... is, "What >does a woman want? Sigmund Freud > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on >its contents: to do so is strictly prohibited and may be unlawful. >Please inform me that this message has gone astray before deleting it. >Thank you for your co-operation -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From jlinda <@t> ces.clemson.edu Wed Apr 25 07:36:26 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Wed Apr 25 07:36:32 2007 Subject: [Histonet] RE: AUTOMATED BLOCK AND SLIDE STORAGE Message-ID: <6.2.3.4.2.20070425083205.043b7698@mailhost.ces.clemson.edu> Mary Ann, You asked: "Does anyone know of an automated or semi automated system for the storage of blocks and slides?" Southern Business Systems has a system designed especially for pathology. Go to: http://www.sbssolutions.com/03_products/auto.shtml and check it out. Linda From Barry.R.Rittman <@t> uth.tmc.edu Wed Apr 25 07:51:03 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Apr 25 07:51:08 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: Sonya I must agree with Bryan that this is a small artery cut at an oblique angle just as it bends. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Wednesday, April 25, 2007 7:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] An histology puzzle? I have an image of an H&E stained structure that I want to submit to a micrograph competition (it is to be judged on artistic rather than scientific merit!). Does anyone know what it might be? It was in an FFPE mouse kidney section - but not within the kidney tissue. Is it a stucture or an artifact? Please go to www.flickr.com/photos/sonzer to see it. Sonya ;-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Apr 25 08:00:56 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Apr 25 08:01:05 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4077@exchsrv01.barrynet.barry.edu> Artery with branch. Probably an interlobar artery with an arcuate artery branching off at the bottom of the picture. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Wednesday, April 25, 2007 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] An histology puzzle? I have an image of an H&E stained structure that I want to submit to a micrograph competition (it is to be judged on artistic rather than scientific merit!). Does anyone know what it might be? It was in an FFPE mouse kidney section - but not within the kidney tissue. Is it a stucture or an artifact? Please go to www.flickr.com/photos/sonzer to see it. Sonya ;-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From stamptrain <@t> yahoo.com Wed Apr 25 08:01:13 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed Apr 25 08:01:21 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <15872.15255.qm@web55810.mail.re3.yahoo.com> It appears to be a highly contracted artery or arteriole with artifactual damage to the surrounding tissue. The smooth muscle cells also appear to have vacuoles or fixation- or mechanical-related artifacts. The angle of cut through the vessel seems tangential--giving the interesting image. Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals Inc Ridgefield, CT --- "Martin S." wrote: > I have an image of an H&E stained structure that I > want to submit to a > micrograph competition (it is to be judged on > artistic rather than > scientific merit!). Does anyone know what it might > be? > > It was in an FFPE mouse kidney section - but not > within the kidney > tissue. > > Is it a stucture or an artifact? > > Please go to www.flickr.com/photos/sonzer to see it. > > > Sonya ;-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ian.montgomery <@t> bio.gla.ac.uk Wed Apr 25 08:20:09 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Apr 25 08:20:21 2007 Subject: [Histonet] An histology puzzle? References: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <001e01c7873c$73edcba0$4724d182@ibls.gla.ac.uk> Sonya, Structure. Blood vessel that ranges from T.S., oblique to 'en face'. An interesting micrograph both artistically and scientifically for teaching. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Martin S." To: Sent: Wednesday, April 25, 2007 1:19 PM Subject: [Histonet] An histology puzzle? I have an image of an H&E stained structure that I want to submit to a micrograph competition (it is to be judged on artistic rather than scientific merit!). Does anyone know what it might be? It was in an FFPE mouse kidney section - but not within the kidney tissue. Is it a stucture or an artifact? Please go to www.flickr.com/photos/sonzer to see it. Sonya ;-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Apr 25 09:03:50 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Apr 25 09:03:10 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <462F5FC6.7030300@umdnj.edu> It is an artery cut at an odd angle (or at a bend). The smoothe muslce of the media and the connective tissue of the adventitia show very well. Geoff Martin S. wrote: >I have an image of an H&E stained structure that I want to submit to a >micrograph competition (it is to be judged on artistic rather than >scientific merit!). Does anyone know what it might be? > >It was in an FFPE mouse kidney section - but not within the kidney >tissue. > >Is it a stucture or an artifact? > >Please go to www.flickr.com/photos/sonzer to see it. > > >Sonya ;-) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From sonya.martin <@t> soton.ac.uk Wed Apr 25 09:25:04 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Apr 25 09:25:25 2007 Subject: [Histonet] RE: An histology puzzle? - Thanks! Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3577@ISS-CL-EX-V1.soton.ac.uk> Thanks for everyones answers - it appears that it is an artery (cut tangentially or on a bend)- obvious to many, but an eye opener for me! Thanks again! Sonya From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 25 09:52:32 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 25 09:52:44 2007 Subject: [Histonet] An histology puzzle? Message-ID: <407F05A128805F4C879A33DBA32E618E20C2D3@TRFT-EX01.xRothGen.nhs.uk> Absolutely. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bhewlett@cogeco.ca Sent: 25 April 2007 13:30 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: re: [Histonet] An histology puzzle? > Sonja, The photo is of an artery cut on an angle through a bend! Bryan > I have an image of an H&E stained structure that I want to submit to a > micrograph competition (it is to be judged on artistic rather than > scientific merit!). Does anyone know what it might be? > > It was in an FFPE mouse kidney section - but not within the kidney > tissue. > > Is it a stucture or an artifact? > > Please go to www.flickr.com/photos/sonzer to see it. > > > Sonya ;-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Apr 25 10:05:21 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Apr 25 10:05:29 2007 Subject: [Histonet] RE: An histology puzzle? - Thanks! In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3577@ISS-CL-EX-V1.soton.ac.uk> Message-ID: Sonya A lot of individuals have a problem with extrapolating 2 dimensional images to 3 dimensions. We always give demonstrations to our students of what images you can see if you take a tube with a side branch or a division into two tubes and section it from various angles. You get a lot of interesting images and with oblique cuts of vessels a false impression of the thickness of the wall. Histology texts such as Gartner and Hiatt also have nice diagrams in the introductory chapter. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Wednesday, April 25, 2007 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: An histology puzzle? - Thanks! Thanks for everyones answers - it appears that it is an artery (cut tangentially or on a bend)- obvious to many, but an eye opener for me! Thanks again! Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Wed Apr 25 10:13:33 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Apr 25 10:14:48 2007 Subject: [Histonet] pre filled bouin's Message-ID: Hi Friends I need to find small specimen bottles that come pre filled with bouin's solution. Does anyone have a supplier? I want to avoid making up my own bottles and the NFPA blah blah blah. Becky Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 From EWURDAK <@t> CSBSJU.EDU Wed Apr 25 10:16:04 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Wed Apr 25 10:16:15 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3576@ISS-CL-EX-V1.soton.ac.uk> Message-ID: It's a blood vessel: a small artery that is turning in the plane of section. The section is tangential to the wall and grazes the smoooth muscle of the tunica media. Liz On 4/25/07 7:19 AM, "Martin S." wrote: > www.flickr.com/photos/sonzer From jmahoney <@t> alegent.org Wed Apr 25 10:26:21 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Apr 25 10:26:39 2007 Subject: [Histonet] pre filled bouin's In-Reply-To: References: Message-ID: <462F2CCD0200003C0000BEFD@gwia.alegent.org> Polyscientific 800-645-5825 >>> "Orr, Rebecca" 04/25/2007 10:13 AM >>> Hi Friends I need to find small specimen bottles that come pre filled with bouin's solution. Does anyone have a supplier? I want to avoid making up my own bottles and the NFPA blah blah blah. Becky Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Apr 25 10:34:03 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 25 10:34:18 2007 Subject: [Histonet] An histology puzzle? In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2D3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <003501c7874f$2865e100$6501a8c0@Patsy> Yep, I agree with Bryan. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, April 25, 2007 8:53 AM To: bhewlett@cogeco.ca; Martin S.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] An histology puzzle? Absolutely. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bhewlett@cogeco.ca Sent: 25 April 2007 13:30 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: re: [Histonet] An histology puzzle? > Sonja, The photo is of an artery cut on an angle through a bend! Bryan > I have an image of an H&E stained structure that I want to submit to a > micrograph competition (it is to be judged on artistic rather than > scientific merit!). Does anyone know what it might be? > > It was in an FFPE mouse kidney section - but not within the kidney > tissue. > > Is it a stucture or an artifact? > > Please go to www.flickr.com/photos/sonzer to see it. > > > Sonya ;-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Wed Apr 25 10:39:15 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Apr 25 10:39:24 2007 Subject: [Histonet] RE: An histology puzzle? - Thanks! References: Message-ID: <004c01c7874f$e229b9e0$4724d182@ibls.gla.ac.uk> Sonya, Like Barry, I do a similar thing but use fruit. Take a banana, cut it in various ways then let the students see the numerous views of the orientation beside the one they usually see. An orange is also good, plus it lets you feed the eager students and have a wee nibble yourself. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Rittman, Barry R" To: Sent: Wednesday, April 25, 2007 4:05 PM Subject: RE: [Histonet] RE: An histology puzzle? - Thanks! Sonya A lot of individuals have a problem with extrapolating 2 dimensional images to 3 dimensions. We always give demonstrations to our students of what images you can see if you take a tube with a side branch or a division into two tubes and section it from various angles. You get a lot of interesting images and with oblique cuts of vessels a false impression of the thickness of the wall. Histology texts such as Gartner and Hiatt also have nice diagrams in the introductory chapter. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Wednesday, April 25, 2007 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: An histology puzzle? - Thanks! Thanks for everyones answers - it appears that it is an artery (cut tangentially or on a bend)- obvious to many, but an eye opener for me! Thanks again! Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Apr 25 10:43:10 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Apr 25 10:43:20 2007 Subject: [Histonet] Processing lymph nodes In-Reply-To: <003501c7874f$2865e100$6501a8c0@Patsy> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8A6B71C@EXCHANGECLUSTER.yumaregional.local> Was wondering what protocols others were doing for processing lymph nodes??? We are getting the partced earth affect. We are also having issues with fall off after cutting, but we have tried various procedures with heating the slides, drying over night, ect to get the water out but we are still seeing fall off. Any takers Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, April 25, 2007 8:34 AM To: 'Marshall Terry Dr, Consultant Histopathologist'; bhewlett@cogeco.ca; 'Martin S.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] An histology puzzle? Yep, I agree with Bryan. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, April 25, 2007 8:53 AM To: bhewlett@cogeco.ca; Martin S.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] An histology puzzle? Absolutely. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bhewlett@cogeco.ca Sent: 25 April 2007 13:30 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: re: [Histonet] An histology puzzle? > Sonja, The photo is of an artery cut on an angle through a bend! Bryan > I have an image of an H&E stained structure that I want to submit to a > micrograph competition (it is to be judged on artistic rather than > scientific merit!). Does anyone know what it might be? > > It was in an FFPE mouse kidney section - but not within the kidney > tissue. > > Is it a stucture or an artifact? > > Please go to www.flickr.com/photos/sonzer to see it. > > > Sonya ;-) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 25 11:06:06 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 25 11:06:12 2007 Subject: [Histonet] RE: An histology puzzle? - Thanks! Message-ID: <407F05A128805F4C879A33DBA32E618E20C2D5@TRFT-EX01.xRothGen.nhs.uk> To re-enforce what Barry said: Some will have noticed that most answers referred to the tangential cut. Having spent a lifetime looking at sections, it didn't even register. That is, I am so used to recognising funny cuts and angles that I give not a second thought to it. However, give me a skin cut parallel to the surface, and I will sink, because I'm not used to that view. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: 25 April 2007 16:05 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: An histology puzzle? - Thanks! Sonya A lot of individuals have a problem with extrapolating 2 dimensional images to 3 dimensions. We always give demonstrations to our students of what images you can see if you take a tube with a side branch or a division into two tubes and section it from various angles. You get a lot of interesting images and with oblique cuts of vessels a false impression of the thickness of the wall. Histology texts such as Gartner and Hiatt also have nice diagrams in the introductory chapter. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Wednesday, April 25, 2007 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: An histology puzzle? - Thanks! Thanks for everyones answers - it appears that it is an artery (cut tangentially or on a bend)- obvious to many, but an eye opener for me! Thanks again! Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SCOTT.TURNER <@t> SPCORP.COM Wed Apr 25 11:13:20 2007 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Wed Apr 25 11:13:45 2007 Subject: [Histonet] Re: Two questions about double IHC In-Reply-To: <9A919A5D70313A4D9C56A025710874082907A0@kenmsg40.us.schp.com> Message-ID: <9A919A5D70313A4D9C56A025710874082907A2@kenmsg40.us.schp.com> I thought about it some more and I think I made this too complicated. If you elute the immune complex after the first antibody, you don't need to precomplex your second primary with the secondary Ab because there won't be any of the first antibody for the secondary to detect. In my original post I said to do both--in actuality only one or the other is necessary. I think you could do this using only the two different TSA kits: the fluorescyl-tyramide kit and the biotin kit. Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Turner, Scott Sent: Tuesday, April 24, 2007 4:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Two questions about double IHC I think there might be a way to do this. The tyramide doesn't actually bind to the immune complex but rather precipitates and covalently binds to the tyrosine residues in the tissue proteins. Therefore, I think you could use the non-biotin fluorescyl-tyramide kit to stain your first antibody, then elute the immune complex leaving the fluorescyl-tyramide behind. Then you'd have to get a little tricky. Since both of your antibodies are rat anti-mouse, you'll have to pre-label your other primary somehow. The easiest thing to do would be to biotinylate that primary. Alternatively, you could pre-complex your biotinylated secondary Ab with your second primary and block the unbound secondary Ab with mouse serum so that it doesn't stick to your first primary antibody. You can figure out the concentrations that work by yourself or you could use the biotinylation and blocking reagents from the Dako ARK. After you've done that, you can put the primary/secondary complex on the tissue and follow with your biotin-based TSA and a Streptavidin-Texas red. Anyway, that's what I could come up with. I know it sounds really complicated but I think it might work (with a LOT of trial and error to get it right). Does anyone out there have any input on this? Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Tuesday, April 24, 2007 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two questions about double IHC Amy, you asked: >snip< I need to do double IHC on FFPE mouse tissue. Unluckily two antibodies I have to use are all rat anti-mouse. The protocol for these antibodies are very similar: Pro K digestion, Primary antibody, Rb anti-Rat 2ND antibody and the rest is following PerkinElmer TSA biotin system instruction (Streptavidin-HRP, biotinylated-tyramide and Streptavidin-HRP). I will perform the two IHC sequentially. I have two questions: 1. Do I need to do Proteinase K for the second antigen? 2. Can I keep all other steps the same and just use Streptavidin-FITC or Streptavidin-Texas Red at the final step to differentiate two antigen? BTW, could you recommend a good website to learn double IHC? >snip< You cannot do the double staining as you describe. By using the same detection method for both, you will end up labeling both antibodies with both colors. If you try eluting the first antigen-antibody complex after labeling with the first antibody, you will lose your first label and will only show labeling of the second. (does this make sense?) It's best if you have two different animal species, or use two different methods for detection. Having both from the same species AND having both require tyramide amplification makes your task next to impossible by fluorescence. It may be possible to do this chromogenically using DAB in a sequential staining protocol, but it's going to be complicated. It's even more complex if you think you will have co-localization. I'll take a stab at it putting something together that might work, but I'm hoping Chris van der Loos (if he's on list) or Gayle Callis comes up with something easier: Primary antibody #1 labeling: Pro K digestion, rat anti-mouse primary Ab 1, biotinylated Rabbit anti-rat, Streptavidin-HRP, biotinylated-tyramide, Streptavidin-HRP, DAB. Elute using heat-induced antigen retrieval (shouldn't need PK digestion at this point) Rat anti-mouse secondary Ab 2, HRP-labeled anti-rat antibody, FITC-tyramide, anti-FITC AP, NBT/BCIP or other stable chromogen Nuclear fast red counterstain (If the above chromogens used). Co-localization will be impossible to see with this color combination. You will need adequate controls doing this experiment. That could potentially be a lot of slides with one experiment. It might be easier to try to optimize one of your antibodies with a higher concentration and no tyramide. Have you tried not using tyramide? Sometimes using polymer HRP systems work well. Look for anti-rat polymer, I think Biocare Medical might have some? Or replace one of the antibodies with one made in a different species (should be much easier), and then try to optimize it without tyramide. Or use labeled antibodies, one with biotin, one with FITC, and use two different detections. You're going to have to simplify this before you can really get good results that you can trust. Best reference I know is Chris van der Loos' book on Multiple Immunostaining, ISBN 1-85996-187-8. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From wasielewski.reinhard.von <@t> mh-hannover.de Wed Apr 25 11:19:03 2007 From: wasielewski.reinhard.von <@t> mh-hannover.de (wasielewski.reinhard.von@mh-hannover.de) Date: Wed Apr 25 11:19:08 2007 Subject: [Histonet] Question again Message-ID: <462F9B97.25815.6EB8AD4@wasielewski.reinhard.von.mh-hannover.de> Hi Histonetters, now we know "it is an artery", I can place my question again: Which is your favourite antibody to stain macrophages and histocytes in FFPE mouse tissue ? Sorry to put the question again, but I sort of missed any reply to my first attempt some days ago. Thanks in advance ("an artery") Reinhard PD Dr. med. Reinhard von Wasielewski From ander093 <@t> tc.umn.edu Wed Apr 25 11:26:45 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Apr 25 11:27:08 2007 Subject: [Histonet] tissue processing In-Reply-To: References: <648217.27829.qm@web61222.mail.yahoo.com> Message-ID: Sheehan states "at least 15 to 20 volumes of fixative should be used for every volume of tissue" pg. 49---in the "Tissue-Handling fixation chart". LuAnn At 07:53 AM 4/24/2007, Rittman, Barry R wrote: >Ren? raises an interesting point. >While we all accept the 20:1 ratio for fixation >as our standard to aim for, I do not believe >that this has actually been studied or published >as original paper. Please let me know if I am incorrect in this statement. >The 20:1 ratio is one of those items that are always passed along. >In fact it has been suggested that the >concentration of the main fixing agent (within >certain limits) and the time of fixation are >more important considerations. This principal is >used in tanning leather when the amount of >glutaraldehyde is carefully matched to the >quantity of the proteins in the leather. The aim >is to have just the right amount of >glutaraldehyde. Excess glutaraldehyde would end >up permeating various parts of our anatomy close to the billfold for example. >In most labs we only carry out a partial >fixation. This is necessitated by time constraints, immunohistochemistry etc. >An alternative to immersion fixation for small >or thin sample sis vapor fixation. This has the >added advantages that no carrier vehicle is >necessary, small amount of sometimes expensive fixing agents can be used. >Barry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Tuesday, April 24, 2007 7:18 AM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] tissue processing > >If you are using a tissue processor (TP) you do >not have to worry about this problem but since >you asked it seems that you are going to process manually. > If that is the case you should maintain the > same volume ratio of 20:1 as when fixing. > The other thing (if processing tissue > manually) is that you will not have agitation, > pressure, vacuum or controlled temperature, so > time has to be increased (as compared with an automatic TP) for each step. > Volume alone will not determine proper > processing without the other advantages of a TP > and these disadvantages have to be compensated with longer steps. > Ren? J. >mwstarbu@mdanderson.org wrote: > Hello, > >Is there a standard tissue volume to liquid volume ratio that you use when >dehydrating samples with ethanol? >Thank you in advance. > >Mike >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Gonzalez <@t> cellgenesys.com Wed Apr 25 11:39:20 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Wed Apr 25 11:39:30 2007 Subject: [Histonet] RE: IHC sausage/checkerboard controls In-Reply-To: <20070421171756.9221727EC35@mail.cellgenesys.com> References: <20070421171756.9221727EC35@mail.cellgenesys.com> Message-ID: Laurie, I don't know if you would consider this a decent price, but we've ordered from Pantomics (San Francisco) http://www.pantomics.com They seems to have frequent "specials" Melissa ------------------------------ Message: 5 Date: Fri, 20 Apr 2007 11:26:33 -0700 From: "Laurie Colbert" Subject: [Histonet] IHC sausage/checkerboard controls To: Message-ID: <57BE698966D5C54EAE8612E8941D76831999F2@EXCHANGE3.huntingtonhospital.com > Content-Type: text/plain; charset="iso-8859-1" Does anyone know where I can purchase sausage and/or checkerboard controls at a decent price?? Laurie Colbert Huntington Hospital Pasadena, CA From TJJ <@t> Stowers-Institute.org Wed Apr 25 11:44:06 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Apr 25 11:44:28 2007 Subject: [Histonet] Re: Two questions about double IHC Message-ID: Scott wrote: I think there might be a way to do this. The tyramide doesn't actually bind to the immune complex but rather precipitates and covalently binds to the tyrosine residues in the tissue proteins. Therefore, I think you could use the non-biotin fluorescyl-tyramide kit to stain your first antibody, then elute the immune complex leaving the fluorescyl-tyramide behind. Then you'd have to get a little tricky. Since both of your antibodies are rat anti-mouse, you'll have to pre-label your other primary somehow. The easiest thing to do would be to biotinylate that primary. Alternatively, you could pre-complex your biotinylated secondary Ab with your second primary and block the unbound secondary Ab with mouse serum so that it doesn't stick to your first primary antibody. You can figure out the concentrations that work by yourself or you could use the biotinylation and blocking reagents from the Dako ARK. After you've done that, you can put the primary/secondary complex on the tissue and follow with your biotin-based TSA and a Streptavidin-Texas red. Anyway, that's what I could come up with. I know it sounds really complicated but I think it might work (with a LOT of trial and error to get it right). Does anyone out there have any input on this? Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA >snip< Actually that does sound doable although still complicated. I'm always concerned about residual molecules of the first binding with the detection of the second, or losing some of the signal of the first after elution. It would take a lot of work to make sure what you're seeing is real. Nice job, Scott. If you can get this simplified, using one biotinylated primary antibody and separate detections systems, I think that's the best. I think Albert Einstein had it right when he said: "Make everything as simple as possible, but not simpler." Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From rsrichmond <@t> aol.com Wed Apr 25 11:53:31 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Apr 25 11:53:40 2007 Subject: [Histonet] Re: Finding Lymph Nodes After Fixation In-Reply-To: <200704251140.1ef462f7667150@rly-yd01.mx.aol.com> References: <200704251140.1ef462f7667150@rly-yd01.mx.aol.com> Message-ID: <8C9557163E397CC-138C-8694@FWM-M43.sysops.aol.com> Jason Wiese notes: Presto-fixe II Active ingredients: 1.0 Molar Formaldehyde 0.2 Molar Glyoxal 60% Methanol Buffer PVP [polyvinylpyrrolidone?] (macromolecular colloidal membrane protectant) This is a totally irrational proprietary formula of the sort John Kiernan used to rail against - where's John now that we need him? If it works as a disclosing fixative or postfixative, it's because it contains an alcohol and formaldehyde. Neutral buffering isn't desirable with such fixatives, but who knows what pH it's buffered to? - The glyoxal is an additional aldehyde fixative, and I can think of no reason to mix them - you probably get the disadvantages of both. People have put things like PVP and middle range Carbowaxes into fixatives for a long time, but I'm not sure they do any good. - You have no way of knowing how the fixative supports immunohistochemistry, and it would take long and expensive trials to find out - and wouldn't be FDA approved for breast cancer anyway. Methyl salicylate (oil of wintergreen) and cedar oil were once recommended as disclosing fixatives, but they aren't practical - time-consuming, expensive, smelly, and sometimes toxic. Bob Richmond Samurai Pathologist Knoxvill TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Eva_Brosco <@t> ihacares.com Wed Apr 25 12:19:32 2007 From: Eva_Brosco <@t> ihacares.com (Eva Brosco) Date: Wed Apr 25 12:19:41 2007 Subject: [Histonet] Shredding and Vacuoles in frozen sections of skin Message-ID: Hello Everyone, I am the histologist in a new Mohs surgery practice and am seeing terrible artifact in the frozen tissue. It is multi complex (maybe simple) in that the dermis is often blasted apart, glands are vacuoled and not staining and the cells look spindled. I believe most is a result of moisture (my lab is 10 feet from the autoclave room). I am using the AccuEdge high profile blades in a Leica CM150S at - 20. I use Tissue Tek OCT, tissue to matrix, not flat embedding technique (will try soon, its is a good technique) Plus slides at room temp. I have scoped the slide prestain and the artifact is present so it is not stain reagents. The DermPath surgeon is not cauterizing until afte tissue is totally removed from patient. I believe ice crystalls are the culprit but would like to hear opinions. I have looked at tissue artifact on line but have not seen any this degraded. Thank you all in advance. From sbreeden <@t> nmda.nmsu.edu Wed Apr 25 12:27:23 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Apr 25 12:27:28 2007 Subject: [Histonet] NEW MEXICO SOCIETY FOR HISTOLOGY Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4420@nmdamailsvr.nmda.ad.nmsu.edu> A quick reminder that the NMSH Annual Meeting will be held on Saturday, May 12th in Albuquerque at the Sheraton Uptown. A unique "twist" to this year's meeting is a structure of Sponsorship Levels. Our Green Chile sponsorship is for $500 "scholarship" to attend NSH; the Red Chile sponsorship provides two $200 awards to techs to purchase books, sign up for teleconferences, or for use in other educational endeavors for histology. A Turquoise sponsorship provides funding for our newsletter costs for one year, and the Roadrunner sponsor is providing a dinner for members the evening before the meeting. At this time, all sponsorships are taken by our tremendously supportive vendors and the awards for NSH and technical materials are ready to be awarded, the newsletter is set for the year and our members will enjoy a dinner at Buca di Beppo Italian restaurant on Friday evening. It's not too late to register, but the deadline is right out there (May 1st). If you'd like to sign up, please email me ASAYC (as soon as you can) so I can make sure we have a spot for you! New Mexico Society members are eligible for the awards. Our website has a registration form with cost details (and a bargain, at that!). Go to www.nmhisto.org and download the only form (registration) I've figured out how to post! Thanks everyone! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From gcallis <@t> montana.edu Wed Apr 25 13:04:52 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Apr 25 13:04:29 2007 Subject: Fixative volumes RE: [Histonet] tissue processing In-Reply-To: References: <648217.27829.qm@web61222.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070425113352.01b40b20@gemini.msu.montana.edu> We have always opt for the 20:1 volume of fixative for research purposes. Fixative is cheap so we like the "rule" that more is better. In the past, the "plunk and dunk fixation syndrome" was alive and well here. The worst example was a whole (unsliced) femoral head sitting in 100 mls for over a month (not in this lab), sent to us, and when bisected, it was still bloody. Needless to say, the fixation was horrible. We encourage submission to this core histology lab as soon as possible, prefering the next day, and IF the tissue is put into less than a 10:1 ratio, it is changed to fresh fixative to replenish the active fixing ingredient. Too little fixative happens way too often especially when the people who initiate the tissue fixation do NOT and sometimes never seem to understand the concept of good fixation, or don't want to bother. If immunostaining is required, time constraints for optimal fixation are explained but that doesn't always work either. Fortunately, most researchers here do their own immunostaining, so they have to deal with their fixation problems. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 10:26 AM 4/25/2007, you wrote: >Sheehan states "at least 15 to 20 volumes of fixative should be used for >every volume of tissue" >pg. 49---in the "Tissue-Handling fixation chart". >LuAnn > > >At 07:53 AM 4/24/2007, Rittman, Barry R wrote: >>Ren? raises an interesting point. >>While we all accept the 20:1 ratio for fixation as our standard to aim >>for, I do not believe that this has actually been studied or published as >>original paper. Please let me know if I am incorrect in this statement. >>The 20:1 ratio is one of those items that are always passed along. >>In fact it has been suggested that the concentration of the main fixing >>agent (within certain limits) and the time of fixation are more important >>considerations. This principal is used in tanning leather when the amount >>of glutaraldehyde is carefully matched to the quantity of the proteins in >>the leather. The aim is to have just the right amount of glutaraldehyde. >>Excess glutaraldehyde would end up permeating various parts of our >>anatomy close to the billfold for example. >>In most labs we only carry out a partial fixation. This is necessitated >>by time constraints, immunohistochemistry etc. >>An alternative to immersion fixation for small or thin sample sis vapor >>fixation. This has the added advantages that no carrier vehicle is >>necessary, small amount of sometimes expensive fixing agents can be used. >>Barry >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >>Sent: Tuesday, April 24, 2007 7:18 AM >>To: histonet@lists.utsouthwestern.edu >>Subject: Re: [Histonet] tissue processing >> >>If you are using a tissue processor (TP) you do not have to worry about >>this problem but since you asked it seems that you are going to process >>manually. >> If that is the case you should maintain the same volume ratio of 20:1 >> as when fixing. >> The other thing (if processing tissue manually) is that you will not >> have agitation, pressure, vacuum or controlled temperature, so time has >> to be increased (as compared with an automatic TP) for each step. >> Volume alone will not determine proper processing without the other >> advantages of a TP and these disadvantages have to be compensated with >> longer steps. >> Ren? J. >>mwstarbu@mdanderson.org wrote: >> Hello, >> >>Is there a standard tissue volume to liquid volume ratio that you use when >>dehydrating samples with ethanol? >>Thank you in advance. >> >>Mike >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >>--------------------------------- >>Ahhh...imagining that irresistible "new car" smell? >> Check outnew cars at Yahoo! Autos. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Apr 25 13:41:52 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 25 13:41:59 2007 Subject: [Histonet] Question again In-Reply-To: <462F9B97.25815.6EB8AD4@wasielewski.reinhard.von.mh-hannover.de> Message-ID: <008901c78769$652248d0$6501a8c0@Patsy> I use rat anti-ms F4/80 from Serotec for macs on mouse tissue. Patsy Also DAKO CD68 PGM1 clone works for macs and monocytes on ms tissue but it is a ms antibody so you have to deal with ms on ms issues. For CD68 be sure to use the PGM1 clone, the others stain a lot more than just macraphages and monocytes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wasielewski.reinhard.von@mh-hannover.de Sent: Wednesday, April 25, 2007 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question again Hi Histonetters, now we know "it is an artery", I can place my question again: Which is your favourite antibody to stain macrophages and histocytes in FFPE mouse tissue ? Sorry to put the question again, but I sort of missed any reply to my first attempt some days ago. Thanks in advance ("an artery") Reinhard PD Dr. med. Reinhard von Wasielewski _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kshimp <@t> seattlecca.org Wed Apr 25 15:35:35 2007 From: kshimp <@t> seattlecca.org (Shimp, Kristen R) Date: Wed Apr 25 15:35:40 2007 Subject: [Histonet] Decal and Fixation of Bone Marrow Biopsies Message-ID: <4EA6CBCAB26218408EFCEC255A88624101E57159@storm.seattlecca.org> We are trying to speed up the decal and fixation for our bone marrow biopsies. Does anyone know of and use a good combination fixative/decal solution? Any other suggestions are welcome. We currently fix them for 4 hours in 10%NBF and Decal for 1:45min. Kristen Shimp, HT (ASCP) Seattle Cancer Care Alliance Pathology Department Desk: 206-288-7801 Main Lab: 206-288-1355 Email: kshimp@seattlecca.org This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From Histosprv06 <@t> aol.com Wed Apr 25 17:49:40 2007 From: Histosprv06 <@t> aol.com (Histosprv06@aol.com) Date: Wed Apr 25 17:49:50 2007 Subject: [Histonet] how to keep toenail on the slide Message-ID: Don't want to step on Joe the Toe's toes (hee hee, ok that was bad)...but I have a different way of cutting toenails that seems to work for us. In our lab we face into the processed block, turn it upside down and put Nair (yes, the hair removal cream) on it for about 3-4 hours (a decent dab). I didn't come up with this, just "inherited" the suggestion and tweeked it a bit. We then wipe it off, spray it with Cytocool and cut it (it's so nice and soft by then) and put it on a plus slide. We leave really hard nails faced up overnight to dry and then reapply the Nair as necessary until it is soft enough. We then "bake" them in the oven for about 30-40 minutes and let them completely cool before staining. We do PAS-F's on the automated stainer this way as well. Our pathologist has not had any problems reading them at all and our slides come out beautiful! Good luck. Kari Zajic, HT,MLT :) ************************************** See what's free at http://www.aol.com. From eileen_dusek <@t> yahoo.com Wed Apr 25 20:39:28 2007 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Wed Apr 25 20:39:32 2007 Subject: [Histonet] Supervisor job at Edward Hospital, Naperville Message-ID: <321727.6079.qm@web50602.mail.re2.yahoo.com> Hi Everyone, Edward Hospital will be searching for a Supervisor in Histology. We are a fast growing Lab in Chicago's western suburbs. We perform ER/PR Quantitation, FISH, IHC and of course routine Histolgy. This is a new position, available July 1, 2007 Thanks Eileen C. Dusek HT Edward Hospital 630-527-3545 --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From jaina.negandhi <@t> sickkids.ca Thu Apr 26 07:56:36 2007 From: jaina.negandhi <@t> sickkids.ca (jaina.negandhi@sickkids.ca) Date: Thu Apr 26 07:56:55 2007 Subject: [Histonet] c-fos staining Message-ID: I will be carrying out c-fos straining, and according to the protocol I am following, I need to preserve my tissue in a mixture of succrose and Thermosol. Thermosol is a preservative, however, I can't seem to find it anywhere (i.e sigma, fluka etc.) Is there another chemical/preservative that I can use instead, or even another technique? Jaina From asmith <@t> mail.barry.edu Thu Apr 26 08:26:16 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Apr 26 08:26:21 2007 Subject: [Histonet] intercalated discs Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4078@exchsrv01.barrynet.barry.edu> Can anyone send me or post the formula and staining and washing procedures for the azocarmine-thionin stain for the intercalated discs of cardiac muscle? Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From burch007 <@t> mc.duke.edu Thu Apr 26 08:52:26 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Apr 26 08:52:32 2007 Subject: [Histonet] intercalated discs In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E4078@exchsrv01.barrynet.barry.edu> Message-ID: Dr. Smith: I know this isn't what you asked for, but you might want to try IHC with N-cadherin. Reacts nicely with the intercalated discs. If you want, I can send you some photographs. JB Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Smith, Allen" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 08:26 AM To cc Subject [Histonet] intercalated discs Can anyone send me or post the formula and staining and washing procedures for the azocarmine-thionin stain for the intercalated discs of cardiac muscle? Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Thu Apr 26 09:18:31 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Apr 26 09:18:53 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: Hi Folks, In our gross room, (and maybe in most-I'm not familiar with the practise) our pathologists are using 1% silver nitrate to see tumor margins in bowels, breast, etc. by pouring the solution onto the tissue prior to slicing. One pathologist has suggested keeping a bucket of 1% AgNO3 in the gross room so that the tissue could instead be briefly immersed. I know that if we use a plastic bucket the plastic will be forever stained which is of course no big deal, but will the plastic compromise the shelf life of the solution itself? Thanks for your time. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. Déclaration de confidentialité Le présent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer complètement de votre système informatique. From gvdobbin <@t> ihis.org Thu Apr 26 09:18:31 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Apr 26 09:19:01 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: Hi Folks, In our gross room, (and maybe in most-I'm not familiar with the practise) our pathologists are using 1% silver nitrate to see tumor margins in bowels, breast, etc. by pouring the solution onto the tissue prior to slicing. One pathologist has suggested keeping a bucket of 1% AgNO3 in the gross room so that the tissue could instead be briefly immersed. I know that if we use a plastic bucket the plastic will be forever stained which is of course no big deal, but will the plastic compromise the shelf life of the solution itself? Thanks for your time. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. Déclaration de confidentialité Le présent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer complètement de votre système informatique. From HParker <@t> Skaggs.Net Thu Apr 26 09:22:09 2007 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu Apr 26 09:23:12 2007 Subject: [Histonet] Histotech needed in Branson, MO In-Reply-To: <20070422170241.1B810AE15@barracuda.skaggs.net> Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A56FE4470@mail1-schc.skaggs.net> Hi Everyone, We are in need of a Histotech (pref ASCP registered) at Skaggs Community Health Center in Branson Missouri. We have a small lab and it is just a two man (woman) team right now. One of our techs is leaving us to move up north and get married. We do approx 500 non-gyn and 5000 surgicals a year. The Tri-Lakes area is a great place to live. It is southwest Missouri and very pretty also is a fisherman's paradise. Plenty of entertainment in town and affordable housing compared to many areas. I enclosed a link to the chamber of commerce here http://www.bransonchamber.com/ Any interested parties can contact me at hparker@skaggs.net and I can frwd your info to the approp people. Thank you, Helayne Parker, H.T. (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Apr 26 09:35:07 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Apr 26 09:35:13 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012475FD@wahtntex2.waht.swest.nhs.uk> Silver Nitrate Poisonous. Causes burns. Long-term exposure can cause permanent blue-grey staining of eyes, mouth, throat and skin, (argyria) and may cause eye damage. Short contact can lead to deposition of black silver stains on the skin. Very destructive of mucous membranes. Skin and eye irritant. Experimental equivocal tumorigenic agent. Toxicity data ORL-MUS LD50 50 mg kg-1 UNR-MAN LDLO 29 mg kg-1 ORL-RBT LDLO 800 mg kg-1 SCU-GPG LDLO 62 mg kg-1 IVN-RBT LDLO 9 mg kg-1 Would you really want a bucket of the stuff lying around for people to dunk things into and increase the surface area for exposure? I would suggest you carry out the procedure in a fume cupboard with small quantities, wearing gloves and lab coat. Mind you blue-grey eyes, mouth, throat and skin might be rather attractive. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From PMonfils <@t> Lifespan.org Thu Apr 26 09:35:47 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Apr 26 09:35:51 2007 Subject: [Histonet] congo red on frozen sections? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C72@LSRIEXCH1.lsmaster.lifespan.org> I was asked to do a congo red for amyloid on a mouse brain that had been formalin fixed, sucrose infiltrated, and frozen. The researcher was certain that this brain would have a high level of amyloid. I used a positive control that was human tissue, formalin fixed, paraffin embedded. The control slide stained perfectly. The mouse brain shows absolutely nothing. Has anyone successfully done congo red staining on frozen sections? Do you see any other possible problems in what I described? From Terry.Marshall <@t> rothgen.nhs.uk Thu Apr 26 09:57:30 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Apr 26 09:57:37 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <407F05A128805F4C879A33DBA32E618E20C2D6@TRFT-EX01.xRothGen.nhs.uk> Wimp:-) Seriously though, I am perpetually perplexed for this modern mania for marking the margins. I think it's a response to blunt knives. Alternatively, and going back to a thread of a couple of weeks ago, a response to cutting tissue before it is fixed and firm, AKA quick turnaround time, AKA keeping ahead of the competition. If you cut with a sharp knife when the tissue is fixed and firm, there will be a flat embedding surface. The real edge of the tissue will be the bit where tissue becomes empty space. Is this so difficult? The suggestion of having buckets of silver nitrate around the place is a species of insanity to my thoughts. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 26 April 2007 15:35 To: Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Silver Nitrate Poisonous. Causes burns. Long-term exposure can cause permanent blue-grey staining of eyes, mouth, throat and skin, (argyria) and may cause eye damage. Short contact can lead to deposition of black silver stains on the skin. Very destructive of mucous membranes. Skin and eye irritant. Experimental equivocal tumorigenic agent. Toxicity data ORL-MUS LD50 50 mg kg-1 UNR-MAN LDLO 29 mg kg-1 ORL-RBT LDLO 800 mg kg-1 SCU-GPG LDLO 62 mg kg-1 IVN-RBT LDLO 9 mg kg-1 Would you really want a bucket of the stuff lying around for people to dunk things into and increase the surface area for exposure? I would suggest you carry out the procedure in a fume cupboard with small quantities, wearing gloves and lab coat. Mind you blue-grey eyes, mouth, throat and skin might be rather attractive. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Thu Apr 26 09:58:13 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Apr 26 09:58:24 2007 Subject: [Histonet] embedding tissue Message-ID: Hi Histonetters, If the forceps wells in an embedding station malfunctions and does not get hot enough, what do you use in an emergency to embed tissue? Bunsen Burner? or Bacti-Cinerator Sterilizer? Helen Johnson (hej01@health.state.ny.state) From PMonfils <@t> Lifespan.org Thu Apr 26 10:14:46 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Apr 26 10:14:52 2007 Subject: [Histonet] embedding tissue In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C73@LSRIEXCH1.lsmaster.lifespan.org> I use an alcohol lamp. The flame is much less hot than that of a bunsen burner. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Helen E Johnson > Sent: Thursday, April 26, 2007 10:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding tissue > > > Hi Histonetters, > If the forceps wells in an embedding station malfunctions and does not > get hot enough, what do you use in an emergency to embed tissue? Bunsen > Burner? or Bacti-Cinerator Sterilizer? > Helen Johnson (hej01@health.state.ny.state) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Jackie.O'Connor <@t> abbott.com Thu Apr 26 10:09:36 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Apr 26 10:16:31 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2D6@TRFT-EX01.xRothGen.nhs.uk> Message-ID: 'Twas always my impression that the reason for marking the surgical margin of a specimen was to determine microscopically that all the tumor had been excised. Lots of surgeons like to cut it really close. "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 09:57 AM To "Kemlo Rogerson" , "Greg Dobbin" , cc Subject RE: [Histonet] Use of 1% AgNO3 in gross room Wimp:-) Seriously though, I am perpetually perplexed for this modern mania for marking the margins. I think it's a response to blunt knives. Alternatively, and going back to a thread of a couple of weeks ago, a response to cutting tissue before it is fixed and firm, AKA quick turnaround time, AKA keeping ahead of the competition. If you cut with a sharp knife when the tissue is fixed and firm, there will be a flat embedding surface. The real edge of the tissue will be the bit where tissue becomes empty space. Is this so difficult? The suggestion of having buckets of silver nitrate around the place is a species of insanity to my thoughts. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 26 April 2007 15:35 To: Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Silver Nitrate Poisonous. Causes burns. Long-term exposure can cause permanent blue-grey staining of eyes, mouth, throat and skin, (argyria) and may cause eye damage. Short contact can lead to deposition of black silver stains on the skin. Very destructive of mucous membranes. Skin and eye irritant. Experimental equivocal tumorigenic agent. Toxicity data ORL-MUS LD50 50 mg kg-1 UNR-MAN LDLO 29 mg kg-1 ORL-RBT LDLO 800 mg kg-1 SCU-GPG LDLO 62 mg kg-1 IVN-RBT LDLO 9 mg kg-1 Would you really want a bucket of the stuff lying around for people to dunk things into and increase the surface area for exposure? I would suggest you carry out the procedure in a fume cupboard with small quantities, wearing gloves and lab coat. Mind you blue-grey eyes, mouth, throat and skin might be rather attractive. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barrickstacey <@t> yahoo.com Thu Apr 26 10:26:38 2007 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Thu Apr 26 10:26:49 2007 Subject: [Histonet] c-fos staining In-Reply-To: Message-ID: <787645.79542.qm@web54309.mail.yahoo.com> Hi Jaina, I did c-fos for a number of years in my old position. Just fix and then put the tissue into 30% sucrose overnight at 4degC before you freeze it for sectioning. We never had a problem and would keep the tissue forever. jaina.negandhi@sickkids.ca wrote: I will be carrying out c-fos straining, and according to the protocol I am following, I need to preserve my tissue in a mixture of succrose and Thermosol. Thermosol is a preservative, however, I can't seem to find it anywhere (i.e sigma, fluka etc.) Is there another chemical/preservative that I can use instead, or even another technique? Jaina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From contact <@t> excaliburpathology.com Thu Apr 26 10:31:55 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Apr 26 10:31:58 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <375981.28172.qm@web50110.mail.re2.yahoo.com> And have you seen the price of silver lately? Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From Terry.Marshall <@t> rothgen.nhs.uk Thu Apr 26 10:32:14 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Apr 26 10:32:19 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <407F05A128805F4C879A33DBA32E618E20C2D8@TRFT-EX01.xRothGen.nhs.uk> Correct. Are you too suggesting you need the edge marked to enable you to see it? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 26 April 2007 16:10 To: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room 'Twas always my impression that the reason for marking the surgical margin of a specimen was to determine microscopically that all the tumor had been excised. Lots of surgeons like to cut it really close. "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 09:57 AM To "Kemlo Rogerson" , "Greg Dobbin" , cc Subject RE: [Histonet] Use of 1% AgNO3 in gross room Wimp:-) Seriously though, I am perpetually perplexed for this modern mania for marking the margins. I think it's a response to blunt knives. Alternatively, and going back to a thread of a couple of weeks ago, a response to cutting tissue before it is fixed and firm, AKA quick turnaround time, AKA keeping ahead of the competition. If you cut with a sharp knife when the tissue is fixed and firm, there will be a flat embedding surface. The real edge of the tissue will be the bit where tissue becomes empty space. Is this so difficult? The suggestion of having buckets of silver nitrate around the place is a species of insanity to my thoughts. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 26 April 2007 15:35 To: Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Silver Nitrate Poisonous. Causes burns. Long-term exposure can cause permanent blue-grey staining of eyes, mouth, throat and skin, (argyria) and may cause eye damage. Short contact can lead to deposition of black silver stains on the skin. Very destructive of mucous membranes. Skin and eye irritant. Experimental equivocal tumorigenic agent. Toxicity data ORL-MUS LD50 50 mg kg-1 UNR-MAN LDLO 29 mg kg-1 ORL-RBT LDLO 800 mg kg-1 SCU-GPG LDLO 62 mg kg-1 IVN-RBT LDLO 9 mg kg-1 Would you really want a bucket of the stuff lying around for people to dunk things into and increase the surface area for exposure? I would suggest you carry out the procedure in a fume cupboard with small quantities, wearing gloves and lab coat. Mind you blue-grey eyes, mouth, throat and skin might be rather attractive. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Thu Apr 26 11:07:38 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Apr 26 11:08:16 2007 Subject: [Histonet] Shredding and Vacuoles in frozen sections of skin In-Reply-To: References: Message-ID: Hi Eva, I train Mohs techs and have seen many artifacts. I am familiar with the Leica 1510S as well. I feel that this is probably the way you are freezing the specimen. I can tell more if I can learn the complete process you use. (how you embed, etc). It would be easier over the phone. Give me a call whenever it is convenient. Best regards, Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Brosco Sent: Wednesday, April 25, 2007 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shredding and Vacuoles in frozen sections of skin Hello Everyone, I am the histologist in a new Mohs surgery practice and am seeing terrible artifact in the frozen tissue. It is multi complex (maybe simple) in that the dermis is often blasted apart, glands are vacuoled and not staining and the cells look spindled. I believe most is a result of moisture (my lab is 10 feet from the autoclave room). I am using the AccuEdge high profile blades in a Leica CM150S at - 20. I use Tissue Tek OCT, tissue to matrix, not flat embedding technique (will try soon, its is a good technique) Plus slides at room temp. I have scoped the slide prestain and the artifact is present so it is not stain reagents. The DermPath surgeon is not cauterizing until afte tissue is totally removed from patient. I believe ice crystalls are the culprit but would like to hear opinions. I have looked at tissue artifact on line but have not seen any this degraded. Thank you all in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dassog <@t> evergreen.edu Thu Apr 26 11:19:11 2007 From: dassog <@t> evergreen.edu (Dasso, Greg (staff)) Date: Thu Apr 26 11:19:16 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room References: <407F05A128805F4C879A33DBA32E618E20C2D8@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <3872E8431D06E545AC955BED177CB6F601604F42@oak.evergreen.edu> Wait, you slosh formalin, osmium tet and halogenated polyaromatics around the bench all day but you wont touch a little silver nitrate? Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marshall Terry Dr,Consultant Histopathologist Sent: Thu 4/26/2007 8:32 AM To: Jackie M O'Connor; Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Correct. Are you too suggesting you need the edge marked to enable you to see it? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 26 April 2007 16:10 To: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room 'Twas always my impression that the reason for marking the surgical margin of a specimen was to determine microscopically that all the tumor had been excised. Lots of surgeons like to cut it really close. "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 09:57 AM To "Kemlo Rogerson" , "Greg Dobbin" , cc Subject RE: [Histonet] Use of 1% AgNO3 in gross room Wimp:-) Seriously though, I am perpetually perplexed for this modern mania for marking the margins. I think it's a response to blunt knives. Alternatively, and going back to a thread of a couple of weeks ago, a response to cutting tissue before it is fixed and firm, AKA quick turnaround time, AKA keeping ahead of the competition. If you cut with a sharp knife when the tissue is fixed and firm, there will be a flat embedding surface. The real edge of the tissue will be the bit where tissue becomes empty space. Is this so difficult? The suggestion of having buckets of silver nitrate around the place is a species of insanity to my thoughts. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 26 April 2007 15:35 To: Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Silver Nitrate Poisonous. Causes burns. Long-term exposure can cause permanent blue-grey staining of eyes, mouth, throat and skin, (argyria) and may cause eye damage. Short contact can lead to deposition of black silver stains on the skin. Very destructive of mucous membranes. Skin and eye irritant. Experimental equivocal tumorigenic agent. Toxicity data ORL-MUS LD50 50 mg kg-1 UNR-MAN LDLO 29 mg kg-1 ORL-RBT LDLO 800 mg kg-1 SCU-GPG LDLO 62 mg kg-1 IVN-RBT LDLO 9 mg kg-1 Would you really want a bucket of the stuff lying around for people to dunk things into and increase the surface area for exposure? I would suggest you carry out the procedure in a fume cupboard with small quantities, wearing gloves and lab coat. Mind you blue-grey eyes, mouth, throat and skin might be rather attractive. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Jones <@t> dako.com Thu Apr 26 11:27:06 2007 From: Sarah.Jones <@t> dako.com (Sarah Jones) Date: Thu Apr 26 11:25:54 2007 Subject: [Histonet] IHC & Special Stains combined Message-ID: <8B07D141BCDE434285DC12B3290E3FB30107D315@exbackca.caus.dako.net> Does anyone out there is Histonetland have any experience with combining IHC and Special Stains? Any thoughts on this? Thanks! Sarah Sarah A. Jones, HTL (ASCP) CM Research Associate, R&D Artisan Special Stains Dako North America, Inc. 6392 Via Real Carpinteria, CA 93013 sarah.jones@dako.com Tel (800) 235-5743 Ext 5681 Fax (805) 684-1372 From Terry.Marshall <@t> rothgen.nhs.uk Thu Apr 26 11:25:53 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Apr 26 11:26:01 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <407F05A128805F4C879A33DBA32E618E20C2D9@TRFT-EX01.xRothGen.nhs.uk> What? I can't even say halogenated polyaromatics:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dasso, Greg (staff) Sent: 26 April 2007 17:19 To: Marshall Terry Dr, Consultant Histopathologist; Jackie M O'Connor; Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Wait, you slosh formalin, osmium tet and halogenated polyaromatics around the bench all day but you wont touch a little silver nitrate? Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marshall Terry Dr,Consultant Histopathologist Sent: Thu 4/26/2007 8:32 AM To: Jackie M O'Connor; Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Correct. Are you too suggesting you need the edge marked to enable you to see it? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 26 April 2007 16:10 To: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room 'Twas always my impression that the reason for marking the surgical margin of a specimen was to determine microscopically that all the tumor had been excised. Lots of surgeons like to cut it really close. "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 09:57 AM To "Kemlo Rogerson" , "Greg Dobbin" , cc Subject RE: [Histonet] Use of 1% AgNO3 in gross room Wimp:-) Seriously though, I am perpetually perplexed for this modern mania for marking the margins. I think it's a response to blunt knives. Alternatively, and going back to a thread of a couple of weeks ago, a response to cutting tissue before it is fixed and firm, AKA quick turnaround time, AKA keeping ahead of the competition. If you cut with a sharp knife when the tissue is fixed and firm, there will be a flat embedding surface. The real edge of the tissue will be the bit where tissue becomes empty space. Is this so difficult? The suggestion of having buckets of silver nitrate around the place is a species of insanity to my thoughts. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 26 April 2007 15:35 To: Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Silver Nitrate Poisonous. Causes burns. Long-term exposure can cause permanent blue-grey staining of eyes, mouth, throat and skin, (argyria) and may cause eye damage. Short contact can lead to deposition of black silver stains on the skin. Very destructive of mucous membranes. Skin and eye irritant. Experimental equivocal tumorigenic agent. Toxicity data ORL-MUS LD50 50 mg kg-1 UNR-MAN LDLO 29 mg kg-1 ORL-RBT LDLO 800 mg kg-1 SCU-GPG LDLO 62 mg kg-1 IVN-RBT LDLO 9 mg kg-1 Would you really want a bucket of the stuff lying around for people to dunk things into and increase the surface area for exposure? I would suggest you carry out the procedure in a fume cupboard with small quantities, wearing gloves and lab coat. Mind you blue-grey eyes, mouth, throat and skin might be rather attractive. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 26 11:34:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 26 11:34:26 2007 Subject: [Histonet] IHC & Special Stains combined In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB30107D315@exbackca.caus.dako.net> Message-ID: <840921.90813.qm@web61220.mail.yahoo.com> Sarah: It will have to depend on the special stain (HC) you are going to do. If the "target tissue" for both HC and IHC is the same, they will interfere. If you just want it as a background color there will be no problem. The thing is that I do not see the usefulness of that combination. Usually IHC targets cells or parts of cells in a very specific way, and HC is used more to stain microorganisms in tissues, or cells in their general tisular arrangement. It could be done if the interference is minimal, but I don't see the advantage of submitting a section to the mechanical "stress" of doing HC and IHC on it. Just a thought though! Ren? J. Sarah Jones wrote: Does anyone out there is Histonetland have any experience with combining IHC and Special Stains? Any thoughts on this? Thanks! Sarah Sarah A. Jones, HTL (ASCP) CM Research Associate, R&D Artisan Special Stains Dako North America, Inc. 6392 Via Real Carpinteria, CA 93013 sarah.jones@dako.com Tel (800) 235-5743 Ext 5681 Fax (805) 684-1372 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From laurie.colbert <@t> huntingtonhospital.com Thu Apr 26 11:52:03 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Apr 26 11:52:20 2007 Subject: [Histonet] CAP Questions regarding microwave leakage and reproducibility Message-ID: <57BE698966D5C54EAE8612E8941D768301268CEA@EXCHANGE3.huntingtonhospital.com> Would these CAP survey questions apply to our lab if we only use the microwave for heating water and agar? We do not process any tissue or perform any special stains in the microwave. Laurie Colbert From rsrichmond <@t> aol.com Thu Apr 26 12:01:56 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Apr 26 12:02:12 2007 Subject: [Histonet] Re: Use of 1% AgNO3 in gross room In-Reply-To: <200704261220.9fa4630d144330@rly-mg02.mx.aol.com> References: <200704261220.9fa4630d144330@rly-mg02.mx.aol.com> Message-ID: <8C9563BBB5EA1BC-138C-D52A@FWM-M43.sysops.aol.com> In my travels to a large number of surgical pathology services back to 1965, I have never seen silver nitrate (AgNO3) used to mark margins, though I've seen references to it in the literature. It seems to me that its environmental problems (and the expense) make it a poor choice. If your pathologists want a tub of something to mark whole specimens with, I suggest india ink (bought cheaply from an art supply store) or black marking ink (bought expensively from the folks that keep the cost of medical care high). I've seen india ink handled this way in a number of labs, though my personal preference is to swab the stuff on. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From llewllew <@t> shaw.ca Thu Apr 26 12:05:21 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Apr 26 12:06:22 2007 Subject: [Histonet] Use of 1% AgNo3 in gross room Message-ID: <002e01c78825$13780a30$6500a8c0@yourlk4rlmsu> I worked in a place where they did this with small breast biopsies. The silver nitrate solution gets all dark and sludgy from tissue chemicals and the formalin. It stops working after a short time. Very expensive and not worth the trouble. Use tattoo inks, much cheaper, safer, prettier, and you can mark each margin a different colour. Bryan Llewellyn ----- Original Message ----- From: "Greg Dobbin" To: Sent: Thursday, April 26, 2007 7:18 AM Subject: [Histonet] Use of 1% AgNO3 in gross room > Hi Folks, > In our gross room, (and maybe in most-I'm not familiar with the > practise) our pathologists are using 1% silver nitrate to see > tumor > margins in bowels, breast, etc. by pouring the solution onto the > tissue > prior to slicing. One pathologist has suggested keeping a bucket > of 1% > AgNO3 in the gross room so that the tissue could instead be > briefly > immersed. I know that if we use a plastic bucket the plastic will > be > forever stained which is of course no big deal, but will the > plastic > compromise the shelf life of the solution itself? > Thanks for your time. > Greg > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or organization. > If you have received this communication in error, please notify the sender > immediately. If you are not the intended recipient, you are not > authorized to use, disclose, distribute, copy, print or rely on this > email, and should promptly delete this email from your entire computer > system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ayant pour objet une personne ou un organisme > particulier. Si vous avez re?u la pr?sente communication par erreur, > veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le > destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, > distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et > vous devriez l'effacer compl?tement de votre syst?me informatique. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From timothy.m.coskran <@t> pfizer.com Thu Apr 26 12:15:57 2007 From: timothy.m.coskran <@t> pfizer.com (Coskran, Timothy M) Date: Thu Apr 26 12:16:14 2007 Subject: [Histonet] IHC and special stains Message-ID: <3F4063B77CC1F445933078B93A0A0C7201E6C697@groamrexm05.amer.pfizer.com> Sarah, I haven't done too much of this combination, but it can definitely be done. I would run your IHC first and then 'counterstain' with the special stain of choice. We've used antibodies to pancreatic hormones and then counterstained with PAS. The PAS does a better job of outlining basement membranes as compared to a standard H&E. I'm not sure if silver stains would be compatible, but I think there are possibilities. Tim Coskran Pfizer ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From benoit.delatour <@t> u-psud.fr Thu Apr 26 12:45:26 2007 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Delatour_Beno=EEt?=) Date: Thu Apr 26 12:45:38 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 41 In-Reply-To: <20070426163327.4B90E629678@smtp1.u-psud.fr> References: <20070426163327.4B90E629678@smtp1.u-psud.fr> Message-ID: <4630E536.7050405@u-psud.fr> Congo red staining can be performed on frozen sections, obtained from cryostat or microtome, without any troubles (see for instance http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16023262&query_hl=2&itool=pubmed_docsum). You can use thioflavin-S as an alternative to Congo red. If results are still negative, it remains possible that amyloid-beta peptides are not aggregated (therefore not stained by the previous dyes) but only present in the tissue as diffuse deposits (you can do IHC with dedicated antibodies to check this possibility). Beno?t > Message: 15 > Date: Thu, 26 Apr 2007 10:35:47 -0400 > From: "Monfils, Paul" > Subject: [Histonet] congo red on frozen sections? > To: > Message-ID: > <4EBFF65383B74D49995298C4976D1D5E273C72@LSRIEXCH1.lsmaster.lifespan.org> > > Content-Type: text/plain; charset="iso-8859-1" > > I was asked to do a congo red for amyloid on a mouse brain that had been formalin fixed, sucrose infiltrated, and frozen. The researcher was certain that this brain would have a high level of amyloid. I used a positive control that was human tissue, formalin fixed, paraffin embedded. The control slide stained perfectly. The mouse brain shows absolutely nothing. Has anyone successfully done congo red staining on frozen sections? Do you see any other possible problems in what I described? > -- Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Email benoit.delatour@u-psud.fr Web http://www.namc.u-psud.fr From benoit.delatour <@t> u-psud.fr Thu Apr 26 12:50:40 2007 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Delatour_Beno=EEt?=) Date: Thu Apr 26 12:50:57 2007 Subject: [Histonet] c-fos In-Reply-To: <20070426163327.4B90E629678@smtp1.u-psud.fr> References: <20070426163327.4B90E629678@smtp1.u-psud.fr> Message-ID: <4630E670.8020409@u-psud.fr> I also confirm that fos IHC can be performed on frozen sections after fixation-cryoprotection without thermosol. If you really need a preservative you can use sodium diazide (http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA). Beno?t histonet-request@lists.utsouthwestern.edu a ?crit : > Message: 8 > Date: Thu, 26 Apr 2007 08:56:36 -0400 > From: jaina.negandhi@sickkids.ca > Subject: [Histonet] c-fos staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > I will be carrying out c-fos straining, and according to the protocol I am > following, I need to preserve my tissue in a mixture of succrose and > Thermosol. Thermosol is a preservative, however, I can't seem to find it > anywhere (i.e sigma, fluka etc.) Is there another chemical/preservative > that I can use instead, or even another technique? > > Jaina > -- Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Email benoit.delatour@u-psud.fr Web http://www.namc.u-psud.fr From Jessica.Vacca <@t> HCAhealthcare.com Thu Apr 26 12:55:46 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Thu Apr 26 12:55:52 2007 Subject: [Histonet] Policy and procedures Message-ID: <41E16A15CE78374EA45B57E0F94339B8024A4E24@ORLEV01.hca.corpad.net> I was wondering how many of you include the product information and MSDS warnings in your policy and procedures on the chemicals that are used. I am reviewing my procedures and this information as well as the manufacturer and catalog numbers are included. I have to do some revisions as well incorporate new ones, and was wondering if all that information is actually required Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From b-frederick <@t> northwestern.edu Thu Apr 26 13:02:57 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 26 13:03:30 2007 Subject: [Histonet] Re: Use of 1% AgNO3 in gross room In-Reply-To: <8C9563BBB5EA1BC-138C-D52A@FWM-M43.sysops.aol.com> Message-ID: <000501c7882d$2300f680$d00f7ca5@lurie.northwestern.edu> After the India ink, a quick dip in Bouin's helps to fix the ink really well! Bernice Frederick HTL (ASCP) Supervisor Senior Research Tech Pathology Core Facility/ECOGPCO-RL Robert H. Lurie Cancer Center Northwestern university Chicago 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Thursday, April 26, 2007 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Use of 1% AgNO3 in gross room In my travels to a large number of surgical pathology services back to 1965, I have never seen silver nitrate (AgNO3) used to mark margins, though I've seen references to it in the literature. It seems to me that its environmental problems (and the expense) make it a poor choice. If your pathologists want a tub of something to mark whole specimens with, I suggest india ink (bought cheaply from an art supply store) or black marking ink (bought expensively from the folks that keep the cost of medical care high). I've seen india ink handled this way in a number of labs, though my personal preference is to swab the stuff on. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SCOTT.TURNER <@t> SPCORP.COM Thu Apr 26 13:09:05 2007 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Thu Apr 26 13:09:38 2007 Subject: [Histonet] c-fos In-Reply-To: <4630E670.8020409@u-psud.fr> Message-ID: <9A919A5D70313A4D9C56A025710874082907A4@kenmsg40.us.schp.com> Are you sure it's "thermosol" and not "thimerosal"? Thimerosal is a commonly used preservative and can be found on the Sigma website (CAS Number: 54-64-8). Thermosol is a process used in commercial dyeing. Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Delatour Beno?t Sent: Thursday, April 26, 2007 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] c-fos I also confirm that fos IHC can be performed on frozen sections after fixation-cryoprotection without thermosol. If you really need a preservative you can use sodium diazide (http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA). Beno?t histonet-request@lists.utsouthwestern.edu a ?crit : > Message: 8 > Date: Thu, 26 Apr 2007 08:56:36 -0400 > From: jaina.negandhi@sickkids.ca > Subject: [Histonet] c-fos staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset=US-ASCII > > I will be carrying out c-fos straining, and according to the protocol > I am following, I need to preserve my tissue in a mixture of succrose > and Thermosol. Thermosol is a preservative, however, I can't seem to > find it anywhere (i.e sigma, fluka etc.) Is there another > chemical/preservative that I can use instead, or even another > technique? > > Jaina > -- Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Email benoit.delatour@u-psud.fr Web http://www.namc.u-psud.fr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From rsrichmond <@t> aol.com Thu Apr 26 13:17:47 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Apr 26 13:18:01 2007 Subject: [Histonet] Re: Use of 1% AgNO3 in gross room In-Reply-To: <000501c7882d$2300f680$d00f7ca5@lurie.northwestern.edu> Message-ID: <8C95646540A5502-138C-DA1F@FWM-M43.sysops.aol.com> Bernice Frederick at theRobert H. Lurie Cancer Center, Northwestern University, Chicago suggests: >>After the India ink, a quick dip in Bouin's helps to fix the ink really well!<< Widely done, though I've never found it helped much - it's much more important to blot the specimen thoroughly dry before inking it. Bouin's fixative is messy and has environmental hazards. Acetone - major fire hazard - is sometimes used after inking also. Supposedly 2% acetic acid (white vinegar diluted about half and half) works quite as well and is not hazardous, but I've never tried it. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Jackie.O'Connor <@t> abbott.com Thu Apr 26 13:33:45 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Apr 26 13:34:25 2007 Subject: [Histonet] c-fos In-Reply-To: <9A919A5D70313A4D9C56A025710874082907A4@kenmsg40.us.schp.com> Message-ID: Thimerosol is a mercury based preservative. Bad stuff. "Turner, Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 01:09 PM To cc Subject RE: [Histonet] c-fos Are you sure it's "thermosol" and not "thimerosal"? Thimerosal is a commonly used preservative and can be found on the Sigma website (CAS Number: 54-64-8). Thermosol is a process used in commercial dyeing. Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Delatour Beno?t Sent: Thursday, April 26, 2007 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] c-fos I also confirm that fos IHC can be performed on frozen sections after fixation-cryoprotection without thermosol. If you really need a preservative you can use sodium diazide ( http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA ). Beno?t histonet-request@lists.utsouthwestern.edu a ?crit : > Message: 8 > Date: Thu, 26 Apr 2007 08:56:36 -0400 > From: jaina.negandhi@sickkids.ca > Subject: [Histonet] c-fos staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset=US-ASCII > > I will be carrying out c-fos straining, and according to the protocol > I am following, I need to preserve my tissue in a mixture of succrose > and Thermosol. Thermosol is a preservative, however, I can't seem to > find it anywhere (i.e sigma, fluka etc.) Is there another > chemical/preservative that I can use instead, or even another > technique? > > Jaina > -- Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Email benoit.delatour@u-psud.fr Web http://www.namc.u-psud.fr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SCOTT.TURNER <@t> SPCORP.COM Thu Apr 26 13:42:33 2007 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Thu Apr 26 13:43:10 2007 Subject: [Histonet] c-fos In-Reply-To: Message-ID: <9A919A5D70313A4D9C56A025710874082907A5@kenmsg40.us.schp.com> Yeah, it's got a particularly bad rap because of its use in vaccines and its possible link to increased incidence of autism. I'm not suggesting it's the best thing to use, simply that the reason it couldn't be found in Sigma or Fluka might be because of a misspelling. Scott Turner Schering-Plough Biopharma (formerly DNAX Research Institute) Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, April 26, 2007 11:34 AM To: Turner, Scott Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] c-fos Thimerosol is a mercury based preservative. Bad stuff. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From alaskagirl1950 <@t> yahoo.com Thu Apr 26 14:08:21 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Apr 26 14:08:28 2007 Subject: [Histonet] Re: Use of 1% AgNO3 in gross room In-Reply-To: <8C95646540A5502-138C-DA1F@FWM-M43.sysops.aol.com> Message-ID: <220270.73436.qm@web52501.mail.re2.yahoo.com> Last hospital that I worked at, the pathologists used vinegar. It did work very well, they would ink the specimen then pat the excess ink off and dip in the vinegar. We just changed the vinegar when it started to take on a lot of color. Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Apr 26 14:23:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 26 14:23:42 2007 Subject: [Histonet] CAP Questions regarding microwave leakage and reproducibility In-Reply-To: <57BE698966D5C54EAE8612E8941D768301268CEA@EXCHANGE3.huntingtonhospital.com> Message-ID: <785463.22689.qm@web61218.mail.yahoo.com> Leakage refers to the potentially harmful activity of MW on the human body, is oven dependent and procedure independent, meaning that can be produced (if the leakage takes places) regardless of the oven usage. For just boiling water, that is something you can "see", I don't believe reproducibility should not be an issue. Just my opinion (as always)! Ren? J. Laurie Colbert wrote: Would these CAP survey questions apply to our lab if we only use the microwave for heating water and agar? We do not process any tissue or perform any special stains in the microwave. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Thu Apr 26 14:29:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 26 14:29:53 2007 Subject: [Histonet] Policy and procedures In-Reply-To: <41E16A15CE78374EA45B57E0F94339B8024A4E24@ORLEV01.hca.corpad.net> Message-ID: <119386.35488.qm@web61221.mail.yahoo.com> Since MSDS are available to all by just pulling the "3 rings binders" I did not include them in the SOP, neither the catalog numbers of the reagents since frequently we had to change vendors due to bulk contracts with the suppliers. I just included the procedure and a bibliographic reference for each (also my SOP was beautifully illustrated with photomicrographs of each procedure, it was gorgeous indeed!). Ren? J. Vacca Jessica wrote: I was wondering how many of you include the product information and MSDS warnings in your policy and procedures on the chemicals that are used. I am reviewing my procedures and this information as well as the manufacturer and catalog numbers are included. I have to do some revisions as well incorporate new ones, and was wondering if all that information is actually required Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Thu Apr 26 15:01:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 26 15:01:19 2007 Subject: [Histonet] Formalin substitutes Message-ID: <566790.47921.qm@web61224.mail.yahoo.com> Dear colleagues: I have a question: besides BoonFix, GlyoFix, Histochoice, HistoFix, KryoFix, MicroFix, Mirsky'sFixative, NeoFix, NoTox, OmniFix (II and 2000), Prefer, Preserve, SafeFix, StatFix, STF (Streck Tissue Fixative), and UMFIX, which other formalin substitutes you know exist and/or have used? Did you all know that formalin is still the fixing agent of choice for 81% of all histology labs (meaning the only 19% use substitutes)? Ren? J. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From julie.blake <@t> holburn.com Thu Apr 26 15:24:05 2007 From: julie.blake <@t> holburn.com (Julie Blake) Date: Thu Apr 26 15:24:14 2007 Subject: [Histonet] Job Posting - Histologist - GTA, Ontario Message-ID: THE HOLBURN GROUP OF COMPANIES, an expanding research and development firm located in Bowmanville, Ontario is focused on innovative research and development programmes in the fields of diagnostics and therapeutics. The projects we undertake both independently and in collaboration with academia and private industry range from early-stage drug discovery to product delivery. We are looking for results-driven professionals to join our team! HISTOLOGIST As Histologist, reporting to a Team Leader, you will undertake various histological procedures. A minimum three years of histology experience preferred. The successful candidate must have a minimum B.Sc. in Medical Laboratory Sciences or possess a MLT (or equivalent) designation. The incumbent will be highly motivated, flexible and thrive in a fast paced environment. We offer a salary commensurate with skills and experience, a comprehensive benefits package and opportunities for growth and professional development. Please submit a covering letter referencing the position and your curriculum vitae in confidence to hr@holburn.com or by fax to 905-623-6702, attention Human Resources. Thank you in advance for your interest. Only those candidates selected for an interview will be contacted. We are an equal opportunity employer. From smclaugh <@t> fhcrc.org Thu Apr 26 15:58:21 2007 From: smclaugh <@t> fhcrc.org (McLaughlin, Sharon R) Date: Thu Apr 26 15:58:26 2007 Subject: [Histonet] Part-time work Message-ID: <9214508BF36C4D4697A25230CE97EE411B1CD9@groucho.fhcrc.org> Hi to all the histonetters, I am looking for per-diem work in the Seattle area. I would be able to work early mornings; week-ends; or possibly evenings. I have been a histology technician since 1971. I would be willing to cut, special stains, embedding, or anything else that you might need help with. Thank you, Sharon McLaughlin 206-667-6115 From AnthonyH <@t> chw.edu.au Thu Apr 26 18:15:58 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Apr 26 18:16:12 2007 Subject: [Histonet] Policy and procedures Message-ID: We only include a note on a chemical used in a procedure if it is classified as hazardous (according to Criteria of Worksafe Australia). Eg: >From Weigert's Iron Haematoxylin Procedure: 1. 10% Alcoholic Haematoxylin Warning: Flammable - see MSDS 2. Aqueous Ferric Chloride Warning: Corrosive - see MSDS 30% aqueous Ferric Chloride 20ml Distilled water 500ml Conc. Hydrochloric acid 5ml Whereas a solution such as 1% Periodic Acid, which is not classified as hazardous according to Criteria of Worksafe Australia does not have a warning in the procedure manual. We have MSDSs (electronic)for all solutions prepared in our lab and it is from these that we determine the hazardous nature of the solution as well design the label. Each hazardous solution has a label with a watermark symbol to indicate its hazardous nature. This hopefully prevents the "familiarity breeds contempt" syndrome. I suppose that every solution, if swallowed, is dangerous but our philosophy is to highlight only those solutions that are, at the present time, known to be hazardous. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, 27 April 2007 5:30 AM To: Vacca Jessica; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy and procedures Since MSDS are available to all by just pulling the "3 rings binders" I did not include them in the SOP, neither the catalog numbers of the reagents since frequently we had to change vendors due to bulk contracts with the suppliers. I just included the procedure and a bibliographic reference for each (also my SOP was beautifully illustrated with photomicrographs of each procedure, it was gorgeous indeed!). Ren? J. Vacca Jessica wrote: I was wondering how many of you include the product information and MSDS warnings in your policy and procedures on the chemicals that are used. I am reviewing my procedures and this information as well as the manufacturer and catalog numbers are included. I have to do some revisions as well incorporate new ones, and was wondering if all that information is actually required Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Thu Apr 26 19:32:57 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 26 19:32:55 2007 Subject: [Histonet] Policy and procedures References: <41E16A15CE78374EA45B57E0F94339B8024A4E24@ORLEV01.hca.corpad.net> Message-ID: <016301c78863$9a792940$d49eae18@yourxhtr8hvc4p> Jessica, I never put vendors and catalog numbers in procedures. Too many changes. I did that once and had to rewrite all the procedures because 'purchasing" went with another vendor. Never did that again. JTT ----- Original Message ----- From: "Vacca Jessica" To: Sent: Thursday, April 26, 2007 12:55 PM Subject: [Histonet] Policy and procedures I was wondering how many of you include the product information and MSDS warnings in your policy and procedures on the chemicals that are used. I am reviewing my procedures and this information as well as the manufacturer and catalog numbers are included. I have to do some revisions as well incorporate new ones, and was wondering if all that information is actually required Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Apr 26 19:36:47 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 26 19:36:44 2007 Subject: [Histonet] how to keep toenail on the slide References: Message-ID: <01a101c78864$237d4d70$d49eae18@yourxhtr8hvc4p> ouch, get off my toes Kari. We used to use Nair also, but it was taking too long and I just got down right tired of hearing the pathologists complain that their cases were taking too long. JTT ----- Original Message ----- From: To: Sent: Wednesday, April 25, 2007 5:49 PM Subject: Re: [Histonet] how to keep toenail on the slide > Don't want to step on Joe the Toe's toes (hee hee, ok that was bad)...but > I > have a different way of cutting toenails that seems to work for us. In our > lab > we face into the processed block, turn it upside down and put Nair (yes, > the > hair removal cream) on it for about 3-4 hours (a decent dab). I didn't > come > up with this, just "inherited" the suggestion and tweeked it a bit. We > then > wipe it off, spray it with Cytocool and cut it (it's so nice and soft by > then) > and put it on a plus slide. We leave really hard nails faced up overnight > to > dry and then reapply the Nair as necessary until it is soft enough. We > then > "bake" them in the oven for about 30-40 minutes and let them completely > cool > before staining. We do PAS-F's on the automated stainer this way as well. > Our pathologist has not had any problems reading them at all and our > slides > come out beautiful! > Good luck. > Kari Zajic, HT,MLT :) > > > > ************************************** See what's free at > http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Thu Apr 26 21:11:45 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Apr 26 21:11:48 2007 Subject: [Histonet] Formalin substitutes In-Reply-To: <566790.47921.qm@web61224.mail.yahoo.com> Message-ID: <342454.92283.qm@web55803.mail.re3.yahoo.com> Not really that surprised. Getting pathologists to change seems to be in the same league as Xerxes telling the tide to stop. We have done some work to show that Prefer gives equivalent fixation for small samples and might work on larger tissue specimens...that is if anyone showed the least interest. It took over 3 months and pounding on doors to even get the set of slides back!!!! Then there's the use of Bouins for testes; again we (altho' we were just validating published data) that Modified Davidson's is superior to Bouins for testes. Any change? Nope. We are all encouraged to be innovative; to "Lead and Learn". So much for team work, etc. Roger Moretz PhD BI Pharmaceuticals Dept of Toxicology Ridgefield CT PS This is just my current house of mossbacks. I can point to at least 2 previous lives with different mossbacks. --- Rene J Buesa wrote: > Dear colleagues: > I have a question: besides BoonFix, GlyoFix, > Histochoice, HistoFix, KryoFix, MicroFix, > Mirsky'sFixative, NeoFix, NoTox, OmniFix (II and > 2000), Prefer, Preserve, SafeFix, StatFix, > STF (Streck Tissue Fixative), and UMFIX, which > other formalin substitutes you know exist and/or > have used? > Did you all know that formalin is still the fixing > agent of choice for 81% of all histology labs > (meaning the only 19% use substitutes)? > Ren? J. > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Apr 27 01:55:05 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Apr 27 01:55:13 2007 Subject: [Histonet] Formalin substitutes Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012475FE@wahtntex2.waht.swest.nhs.uk> Getting pathologists to change seems to be in the same league as Xerxes telling the tide to stop. Rene Xerxes (the Great) was a Persian Emperor who attempted to bridge the Hellespont and whipped (300x) it when a storm destroyed his bridges; his second attempt to bridge the strait was successful. King Canute (some 1800 years later) sat on the beach, on his thrown, and tried to stop the tide. So I guess your comparison is historically inaccurate IMHO. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Apr 27 02:06:49 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Apr 27 02:06:55 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012475FF@wahtntex2.waht.swest.nhs.uk> "halogenated polyaromatics" The common carp (Cyprinus carpio) offers an apparently good system for monitoring halogenated polyaromatics both by cytochrome P450IA proteins and EROD activity (van der Weiden, Tibosch et al. 1993). A third example was provided by measurements of TCDD and TCDF, in addition to cytochrome P450IA1 in the common carp (Cyprinus carpio) and the cutthroat trout (Onchorhynchus clarkii). Isn't that interesting? In my search to find out exactly what a halogenated polyaromatic (poly- aromatic Terry), I found the common carp has a detection system! It's a bad day when you don't learn something, but why would I want to use dioxin and if I did I'd have a pond nearby with Koi. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Apr 27 02:13:56 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Apr 27 02:14:02 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247600@wahtntex2.waht.swest.nhs.uk> Wimp:-) It's all right for you, your old and your life's nearly over, but think of the Staff!! Those poor sods are instructed by the Pathologist to dunk things in things with total disregard for life and limp. I'm the one suffering from a life of formalin inhalation, zinc ingestion (your fault), lead ingestion (again your fault) and various other toxic cocktails. Is it no wonder I'm sterile, losing my hair and have aching joints? The young are our future (god save us) and with reducing sperm rates we can't afford to poison them any further (g). Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Apr 27 02:17:44 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Apr 27 02:17:50 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247602@wahtntex2.waht.swest.nhs.uk> 'limb' not 'limp' Freudian slip!!! See what you've done! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 27 05:24:15 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Apr 27 05:24:22 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <407F05A128805F4C879A33DBA32E618E20C2DA@TRFT-EX01.xRothGen.nhs.uk> Well, you're quite right in all respects Kemlo. I'll just shuffle my chair a little closer to the coffin I keep close by in case of need, and shut up. Terry PS I'll leave you my testicles if they can be of any service to you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 27 April 2007 08:14 To: Marshall Terry Dr, Consultant Histopathologist; Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room Wimp:-) It's all right for you, your old and your life's nearly over, but think of the Staff!! Those poor sods are instructed by the Pathologist to dunk things in things with total disregard for life and limp. I'm the one suffering from a life of formalin inhalation, zinc ingestion (your fault), lead ingestion (again your fault) and various other toxic cocktails. Is it no wonder I'm sterile, losing my hair and have aching joints? The young are our future (god save us) and with reducing sperm rates we can't afford to poison them any further (g). Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 27 05:26:51 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Apr 27 05:26:56 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <407F05A128805F4C879A33DBA32E618E20C2DB@TRFT-EX01.xRothGen.nhs.uk> Hmm. I'd better leave you my magazines too. Terry (It's Friday here). -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 27 April 2007 08:18 To: Marshall Terry Dr, Consultant Histopathologist; Dasso, Greg (staff); Jackie M O'Connor; Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Use of 1% AgNO3 in gross room 'limb' not 'limp' Freudian slip!!! See what you've done! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Apr 27 05:40:57 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Apr 27 05:41:06 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247609@wahtntex2.waht.swest.nhs.uk> PS I'll leave you my testicles if they can be of any service to you. Terry No thanks, I've seen where they've been! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Apr 27 05:41:53 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Apr 27 05:41:58 2007 Subject: [Histonet] Use of 1% AgNO3 in gross room Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124760A@wahtntex2.waht.swest.nhs.uk> Hmm. I'd better leave you my magazines too. Terry Home brewing and gardening? What the hell am I going to do with them? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From hancockestates <@t> yahoo.com Fri Apr 27 05:46:43 2007 From: hancockestates <@t> yahoo.com (MaryAnn Hancock) Date: Fri Apr 27 05:46:45 2007 Subject: [Histonet] SILVER NITRATE Message-ID: <119491.3433.qm@web51605.mail.re2.yahoo.com> Our Pathologists have used silver nitrate for years a 5% solution and then add NACL before discarding down the drain with water. We only use it on large specimens, breast, lumpectomy etc. MaryAnn Hancock --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From lnlj <@t> novonordisk.com Fri Apr 27 06:00:36 2007 From: lnlj <@t> novonordisk.com (LNLJ (Lene Lyngsie Jensen)) Date: Fri Apr 27 06:00:47 2007 Subject: [Histonet] Proper Decalcification In-Reply-To: Message-ID: Hey all... I have never heard about this machine before. I don't think we have it here I Denmark (small country in Scandinavia:-)). Could someone please tell me a bit more about it, and how you use it? Maybe it would be worth getting it all the way to Denmark? Thanks. Lene Lyngsie Jensen Medical Laboratory Scientific Officer (MLSO**) GLP-1 and Obesity Pharmacology Novo Nordisk A/S Novo Nordisk Park DK-2760 M?l?v Denmark -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 16. april 2007 15:32 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification Faxitron MX 20 Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Monday, April 16, 2007 5:47 AM To: Molinari, Betsy Subject: RE: [Histonet] Proper Decalcification Which model do you use? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, April 16, 2007 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification We use the Faxitron. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Friday, April 13, 2007 10:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Proper Decalcification I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Apr 27 06:21:10 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Apr 27 06:21:18 2007 Subject: [Histonet] Proper Decalcification References: Message-ID: I looked up info. on it at www.faxitron.com Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LNLJ (Lene Lyngsie Jensen) Sent: Friday, April 27, 2007 7:01 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification Hey all... I have never heard about this machine before. I don't think we have it here I Denmark (small country in Scandinavia:-)). Could someone please tell me a bit more about it, and how you use it? Maybe it would be worth getting it all the way to Denmark? Thanks. Lene Lyngsie Jensen Medical Laboratory Scientific Officer (MLSO**) GLP-1 and Obesity Pharmacology Novo Nordisk A/S Novo Nordisk Park DK-2760 M?l?v Denmark -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 16. april 2007 15:32 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification Faxitron MX 20 Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Monday, April 16, 2007 5:47 AM To: Molinari, Betsy Subject: RE: [Histonet] Proper Decalcification Which model do you use? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, April 16, 2007 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification We use the Faxitron. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Friday, April 13, 2007 10:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Proper Decalcification I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Apr 27 06:53:00 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 27 06:54:28 2007 Subject: [Histonet] SILVER NITRATE In-Reply-To: <119491.3433.qm@web51605.mail.re2.yahoo.com> Message-ID: <000001c788c2$9cccf820$fce82d4b@HPPav2> I would suggest you check with your safety department and/or your city's waste department. Silver salt is silver salt, and can not be disposed of down the sink, per the EPA. Chemically, by adding sodium chloride, the clear silver nitrate has been made into silver chloride, which is now white and which is not very soluble in water, hence the precipitation (turning visible). However, it is still a silver salt which cannot be disposed down the sink. Now, if you filter this through a filter paper, collect up the silver chloride on the filter paper, you should at that point be able to throw a away the clear water. You might wanted to have your safety department check the water first, to see if you did remove all the silver salts from the water. Safety department will send it out for analysis. Then, let the filter paper with the silver chloride dry overnight (in a hood is fine - dries faster and it's out of everyone's way). Put the dried filter paper into a zippable plastic bag. As you collect more filter papers with silver on it (such as from disposing solutions of silver stains like retic, GMS, PAMS, etc.) you can put these filter papers together in the same zippable plastic bag. These plastic bags with filter paper with silver chloride can be hauled away by your hazardous waste hauler. Now you are properly disposing silver nitrate via EPA rules, and you are not paying for disposal of water which is very heavy by weight. Another method for disposal of silver nitrate solutions is to add equal amount by volume of 1.0 N hydrocloric acid to the silver solution, and let it set overnight. Again, silver chloride will precipitate out, collect on the filter paper and dried as mentioned above, and the "water" part can now be disposed down the sink (if tested and OK'ed to go this). We take an old 1 gallon plastic alcohol bottle, pour all our silver staining solutions in it until it is 1/2 full, the pour the 1 N Hydrochoric acid to the top, let it set overnight, filter, etc. Then we reuse the plastic bottle for the next collection, as the some silver does stick to the inside plastic and turns black. There's no danger in this, it just looks ugly. After a few times of collecting and precipitating, we have the waste hauler dispose of the bottle too, and just start with a new plastic bottle. This bottle cannot go into the regular trash, as it is contaminated with silver, and the EPA won't like it in a regular land fill. We are a large institution, and are doing lots of silver stains a day, so we collect silver in large quantities. If you are a small institution, collect the silver solutions in a smaller bottle. Ammoniacal silver (such as used in the retic stain), if allowed to dry, can be explosive. So don't let the silver solutions dry out when you are collecting them. Add some water, if needed. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryAnn Hancock Sent: Friday, April 27, 2007 6:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SILVER NITRATE Our Pathologists have used silver nitrate for years a 5% solution and then add NACL before discarding down the drain with water. We only use it on large specimens, breast, lumpectomy etc. MaryAnn Hancock --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Fri Apr 27 06:55:43 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Apr 27 06:56:26 2007 Subject: [Histonet] Speaking of margins... Message-ID: Hi Friends, We use different inks and marking dyes for our margins. We have stopped using bouin's as a mordant to keep the ink on the tissue through processing, and switched over to an acetic acid formulation. Could anyone please share what they use to help keep the ink in place? Bouin's is hands down better but quite stinkish. ~ I had suggested to the residents and PAs that are grossing to perhaps lighten up on the administration of ink....any backers on this? Have a great weekend! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 Message: 1 Date: Thu, 26 Apr 2007 13:01:56 -0400 From: rsrichmond@aol.com Subject: [Histonet] Re: Use of 1% AgNO3 in gross room To: histonet@lists.utsouthwestern.edu Message-ID: <8C9563BBB5EA1BC-138C-D52A@FWM-M43.sysops.aol.com> Content-Type: text/plain; charset="us-ascii"; format=flowed In my travels to a large number of surgical pathology services back to 1965, I have never seen silver nitrate (AgNO3) used to mark margins, though I've seen references to it in the literature. It seems to me that its environmental problems (and the expense) make it a poor choice. If your pathologists want a tub of something to mark whole specimens with, I suggest india ink (bought cheaply from an art supply store) or black marking ink (bought expensively from the folks that keep the cost of medical care high). I've seen india ink handled this way in a number of labs, though my personal preference is to swab the stuff on. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ From sonya.martin <@t> soton.ac.uk Fri Apr 27 07:15:47 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Fri Apr 27 07:16:17 2007 Subject: [Histonet] Friday afternoon brain teasers Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E357C@ISS-CL-EX-V1.soton.ac.uk> Hi All, I have a couple more brain teasers for you - since its Friday! Can anyone identify these structures (1 and 2) - again they appeared in FFPE sections of mouse Kidney www.flickr.com/photos/sonzer Apologies if they're just too easy! Sonya From Jackie.O'Connor <@t> abbott.com Fri Apr 27 07:22:40 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Apr 27 07:23:15 2007 Subject: [Histonet] Formalin substitutes In-Reply-To: <566790.47921.qm@web61224.mail.yahoo.com> Message-ID: STF is no longer being sold. Becton Dickenson Zinc IHC fixative is the next best choice as a replacement for anyone using STF for IHC. I compared STF to BD zinc vs. Prefer - -tissues fixed in Prefer had greatly reduced signal (Murine CD31) than STF and the BD zinc. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 04/26/2007 03:01 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Formalin substitutes Dear colleagues: I have a question: besides BoonFix, GlyoFix, Histochoice, HistoFix, KryoFix, MicroFix, Mirsky'sFixative, NeoFix, NoTox, OmniFix (II and 2000), Prefer, Preserve, SafeFix, StatFix, STF (Streck Tissue Fixative), and UMFIX, which other formalin substitutes you know exist and/or have used? Did you all know that formalin is still the fixing agent of choice for 81% of all histology labs (meaning the only 19% use substitutes)? Ren? J. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Fri Apr 27 07:44:30 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Apr 27 07:44:38 2007 Subject: [Histonet] SILVER NITRATE In-Reply-To: <000001c788c2$9cccf820$fce82d4b@HPPav2> References: <000001c788c2$9cccf820$fce82d4b@HPPav2> Message-ID: Or, for those of us old enough to remember photomicroscopy by wet-chemistry methods, AgCl is used in film negatives and prints for recording images. And, photograph buffs collected their used developed, put a current through it, and collected the metallic silver. Not only made some money, but much less hazardous waste (and nowadays, less hazardous-waste costs). Keep collecting the filters, ash them to get the Ag precipitate free of the paper, and reclaim the metal. I don't have a recipe for doing this handy, but there would be plenty on the web, I'm sure. Phil >I would suggest you check with your safety department and/or your city's >waste department. Silver salt is silver salt, and can not be disposed of >down the sink, per the EPA. > >Chemically, by adding sodium chloride, the clear silver nitrate has been >made into silver chloride, which is now white and which is not very soluble >in water, hence the precipitation (turning visible). However, it is still a >silver salt which cannot be disposed down the sink. > >Now, if you filter this through a filter paper, collect up the silver >chloride on the filter paper, you should at that point be able to throw a >away the clear water. You might wanted to have your safety department check >the water first, to see if you did remove all the silver salts from the >water. Safety department will send it out for analysis. > >Then, let the filter paper with the silver chloride dry overnight (in a hood >is fine - dries faster and it's out of everyone's way). Put the dried filter >paper into a zippable plastic bag. As you collect more filter papers with >silver on it (such as from disposing solutions of silver stains like retic, >GMS, PAMS, etc.) you can put these filter papers together in the same >zippable plastic bag. > >These plastic bags with filter paper with silver chloride can be hauled away >by your hazardous waste hauler. Now you are properly disposing silver >nitrate via EPA rules, and you are not paying for disposal of water which is >very heavy by weight. > >Another method for disposal of silver nitrate solutions is to add equal >amount by volume of 1.0 N hydrocloric acid to the silver solution, and let >it set overnight. Again, silver chloride will precipitate out, collect on >the filter paper and dried as mentioned above, and the "water" part can now >be disposed down the sink (if tested and OK'ed to go this). > >We take an old 1 gallon plastic alcohol bottle, pour all our silver staining >solutions in it until it is 1/2 full, the pour the 1 N Hydrochoric acid to >the top, let it set overnight, filter, etc. Then we reuse the plastic bottle >for the next collection, as the some silver does stick to the inside plastic >and turns black. There's no danger in this, it just looks ugly. After a few >times of collecting and precipitating, we have the waste hauler dispose of >the bottle too, and just start with a new plastic bottle. This bottle cannot >go into the regular trash, as it is contaminated with silver, and the EPA >won't like it in a regular land fill. > >We are a large institution, and are doing lots of silver stains a day, so we >collect silver in large quantities. If you are a small institution, collect >the silver solutions in a smaller bottle. Ammoniacal silver (such as used in >the retic stain), if allowed to dry, can be explosive. So don't let the >silver solutions dry out when you are collecting them. Add some water, if >needed. > >Peggy A. Wenk, HTL(ASCP)SLS >William Beaumont Hospital >Royal Oak, MI 48073 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryAnn >Hancock >Sent: Friday, April 27, 2007 6:47 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] SILVER NITRATE > >Our Pathologists have used silver nitrate for years a 5% solution and then >add NACL before discarding down the drain with water. We only use it on >large specimens, breast, lumpectomy etc. > MaryAnn Hancock > > >--------------------------------- >Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From asmith <@t> mail.barry.edu Fri Apr 27 08:07:44 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Apr 27 08:07:50 2007 Subject: [Histonet] Friday afternoon brain teasers In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E357C@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E407C@exchsrv01.barrynet.barry.edu> Left hand picture (#2) is of collecting ducts in the renal medulla of a rodent. (Human collecting ducts have taller cells.) Center picture is a sympathetic ganglion. From its size and the fat cells around it, I would guess it is the aorticorenal ganglion; ganglia within the kidney are smaller. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Friday, April 27, 2007 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday afternoon brain teasers Hi All, I have a couple more brain teasers for you - since its Friday! Can anyone identify these structures (1 and 2) - again they appeared in FFPE sections of mouse Kidney www.flickr.com/photos/sonzer Apologies if they're just too easy! Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From ian.montgomery <@t> bio.gla.ac.uk Fri Apr 27 09:18:17 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Apr 27 09:18:44 2007 Subject: Fw: [Histonet] Friday afternoon brain teasers Message-ID: <009b01c788d6$e7a9eb00$4724d182@ibls.gla.ac.uk> Structure 1: Ganglion. Structure 2: L.S,ish section of straight tubules. Friday afternoon flaming. Magnification factors, bad idea, I don't believe them, it's not x40 on my monitor. Put a scale bar on the micrographs, you know it makes sense. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Martin S." To: Sent: Friday, April 27, 2007 1:15 PM Subject: [Histonet] Friday afternoon brain teasers Hi All, I have a couple more brain teasers for you - since its Friday! Can anyone identify these structures (1 and 2) - again they appeared in FFPE sections of mouse Kidney www.flickr.com/photos/sonzer Apologies if they're just too easy! Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MBURTON1 <@t> PARTNERS.ORG Fri Apr 27 09:43:46 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Fri Apr 27 09:45:37 2007 Subject: [Histonet] Re: Use of 1% AgNO3 in gross room Message-ID: Yes, we also used very dilute acetic acid solution (1%?). Yet another much better alternative to a bucket of AgNO3 Message: 12 Date: Thu, 26 Apr 2007 12:08:21 -0700 (PDT) From: Patricia Adams Subject: Re: [Histonet] Re: Use of 1% AgNO3 in gross room To: HistoNet Message-ID: <220270.73436.qm@web52501.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Last hospital that I worked at, the pathologists used vinegar. It did work very well, they would ink the specimen then pat the excess ink off and dip in the vinegar. We just changed the vinegar when it started to take on a lot of color. Patricia Adams The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From cormier <@t> MIT.EDU Fri Apr 27 09:45:36 2007 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Fri Apr 27 09:46:28 2007 Subject: [Histonet] ? why does my gallbladder control not stain for bile Message-ID: <002c01c788da$b7c222f0$92003712@mit.edu> Hello All, Why does my gallbladder control not stain for bile? ( the mouse livers do not stain either, but that is not unexpected ) It is freshly cut, the solutions are commercially bought and appropriately stored, the stain seems very straightforward... Shouldn't this stain the bile in the gallbladder? I can see it in the block... all nice dark green...Am I just missing the obvious? Am I clueless? (Don't answer that!) I did this stain like, one other time, in the early 90's and we had a nice piece of liver w/ bile for a control. I have read Carson's and Bancroft's books, and they say the best control is a piece of tissue w/ bile, so the gallbladder should work, right? Help! (and thanks!) Kathy Div Comp Medicine MIT From gu.lang <@t> gmx.at Fri Apr 27 09:50:14 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Apr 27 09:50:15 2007 Subject: AW: [Histonet] Speaking of margins... In-Reply-To: Message-ID: <000c01c788db$5dd2cd20$6412a8c0@dielangs.at> We take a hairdryer and dry the inked tissue until it gets a shiny surface. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orr, Rebecca Gesendet: Freitag, 27. April 2007 13:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Speaking of margins... Hi Friends, We use different inks and marking dyes for our margins. We have stopped using bouin's as a mordant to keep the ink on the tissue through processing, and switched over to an acetic acid formulation. Could anyone please share what they use to help keep the ink in place? Bouin's is hands down better but quite stinkish. ~ I had suggested to the residents and PAs that are grossing to perhaps lighten up on the administration of ink....any backers on this? Have a great weekend! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 Message: 1 Date: Thu, 26 Apr 2007 13:01:56 -0400 From: rsrichmond@aol.com Subject: [Histonet] Re: Use of 1% AgNO3 in gross room To: histonet@lists.utsouthwestern.edu Message-ID: <8C9563BBB5EA1BC-138C-D52A@FWM-M43.sysops.aol.com> Content-Type: text/plain; charset="us-ascii"; format=flowed In my travels to a large number of surgical pathology services back to 1965, I have never seen silver nitrate (AgNO3) used to mark margins, though I've seen references to it in the literature. It seems to me that its environmental problems (and the expense) make it a poor choice. If your pathologists want a tub of something to mark whole specimens with, I suggest india ink (bought cheaply from an art supply store) or black marking ink (bought expensively from the folks that keep the cost of medical care high). I've seen india ink handled this way in a number of labs, though my personal preference is to swab the stuff on. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Fri Apr 27 10:00:37 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Fri Apr 27 10:01:20 2007 Subject: [Histonet] RE Friday afternoon brain teasers Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E357D@ISS-CL-EX-V1.soton.ac.uk> Ok they were too easy for those in the know - I've added a couple more just for fun! Have a good weekend! Sonya From liz <@t> premierlab.com Fri Apr 27 10:14:08 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Apr 27 10:14:19 2007 Subject: [Histonet] Proper Decalcification In-Reply-To: Message-ID: <000001c788de$b4a38100$0d00a8c0@domain.Premier> Faxitrons are quite expensive. I just checked into purchasing one and the small ones range from I think about $20,000 to $50,000 for the digital one. Its nice to have but for me the cost was too much. Here is the link. IMEB used to have a used MX-20 digital for sale, but it still think that it was around $35,000.00. http://faxitron.com/medical.htm Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LNLJ (Lene Lyngsie Jensen) Sent: Friday, April 27, 2007 5:01 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification Hey all... I have never heard about this machine before. I don't think we have it here I Denmark (small country in Scandinavia:-)). Could someone please tell me a bit more about it, and how you use it? Maybe it would be worth getting it all the way to Denmark? Thanks. Lene Lyngsie Jensen Medical Laboratory Scientific Officer (MLSO**) GLP-1 and Obesity Pharmacology Novo Nordisk A/S Novo Nordisk Park DK-2760 M?l?v Denmark -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 16. april 2007 15:32 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification Faxitron MX 20 Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Monday, April 16, 2007 5:47 AM To: Molinari, Betsy Subject: RE: [Histonet] Proper Decalcification Which model do you use? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, April 16, 2007 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proper Decalcification We use the Faxitron. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Friday, April 13, 2007 10:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Proper Decalcification I've been having problems with my PA when it comes to proper decalcification of our bone specimens. They are either over or under decalcified. Do many of you use a chemical end point method to determine the decalcification or do you use other methods? If so, could you tell me the best product to use? I appreciate any feedback. Paula Lucas Lab Manger Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From relia1 <@t> earthlink.net Fri Apr 27 10:27:59 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Apr 27 10:28:03 2007 Subject: [Histonet] Happy Lab Week Message-ID: Hello Histonetters, It sounds like most everyone has had a great Lab Week. It is great to be appreciated for the important work you do. Have a Great Weekend. Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 27 10:30:47 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Apr 27 10:30:55 2007 Subject: [Histonet] Formalin substitutes Message-ID: <407F05A128805F4C879A33DBA32E618E20C2DC@TRFT-EX01.xRothGen.nhs.uk> Amazing isn't it, that whilst all the human diagnostic labs are going for the quickest turnaround times, they use the slowest fixative. Beam me up (the late) Scottie. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 26 April 2007 21:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin substitutes Dear colleagues: I have a question: besides BoonFix, GlyoFix, Histochoice, HistoFix, KryoFix, MicroFix, Mirsky'sFixative, NeoFix, NoTox, OmniFix (II and 2000), Prefer, Preserve, SafeFix, StatFix, STF (Streck Tissue Fixative), and UMFIX, which other formalin substitutes you know exist and/or have used? Did you all know that formalin is still the fixing agent of choice for 81% of all histology labs (meaning the only 19% use substitutes)? Ren? J. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Apr 27 10:38:44 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Apr 27 10:38:20 2007 Subject: [Histonet] Proper Decalcification In-Reply-To: References: Message-ID: <6.0.0.22.1.20070427093223.01b06780@gemini.msu.montana.edu> How to use the FAXITRON for decalcification endpoint checks is found in Gamble and Bancroft Theory and Practice of Histological Technique, 5th edition, 2001, Bone Chapter. The instrument is not cheap but it is the most sensitive way to do a decalcification endpoint. Finding film cans sometimes be a chore, but you can xray many samples at once to make it more efficient. There are other ways to do decalcification endpoints, some simple but very effective. The chemical test is easy, cheap and commonly done, found in histotechnology textbooks. Another is a weight loss/weight gain originally done to check nitric acid decalcification. Since we gave away our FAXITRON (worst thing ever done!) we do the weight loss/weight gain method, but you need a balance that weighs in milligrams. I will be happy to send the latter two privately if needed. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 05:21 AM 4/27/2007, you wrote: >I looked up info. on it at www.faxitron.com > > >Jeanine Bartlett >Infectious Disease Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LNLJ (Lene >Lyngsie Jensen) >Sent: Friday, April 27, 2007 7:01 AM >To: Molinari, Betsy; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Proper Decalcification > >Hey all... > >I have never heard about this machine before. I don't think we have it >here I Denmark (small country in Scandinavia:-)). > >Could someone please tell me a bit more about it, and how you use it? >Maybe it would be worth getting it all the way to Denmark? > > > >Thanks. > > >Lene Lyngsie Jensen >Medical Laboratory Scientific Officer (MLSO**) >GLP-1 and Obesity Pharmacology > >Novo Nordisk A/S >Novo Nordisk Park >DK-2760 M?l?v >Denmark > > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy >Sent: 16. april 2007 15:32 >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Proper Decalcification > > > >Faxitron MX 20 > > > >Betsy Molinari HT (ASCP) > >Texas Heart Institute > >Cardiovascular Pathology > >6770 Bertner Ave. > >Houston,TX 77030 > >832-355-6524 > >832-355-6812 (fax) > > > > > >-----Original Message----- > >From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] > >Sent: Monday, April 16, 2007 5:47 AM > >To: Molinari, Betsy > >Subject: RE: [Histonet] Proper Decalcification > > > >Which model do you use? > > > >Thanks, > > > >Jeanine Bartlett, BS, HT(ASCP)QIHC > >Centers for Disease Control and Prevention > >Infectious Diseases Pathology Branch > >1600 Clifton Road, MS/G-32 > >18/SB-114 > >Atlanta, GA 30333 > >(404) 639-3590 > >jeanine.bartlett@cdc.hhs.gov > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Molinari, Betsy > >Sent: Monday, April 16, 2007 6:41 AM > >To: histonet@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Proper Decalcification > > > >We use the Faxitron. > > > >Betsy Molinari HT (ASCP) > >Texas Heart Institute > >Cardiovascular Pathology > >6770 Bertner Ave. > >Houston,TX 77030 > >832-355-6524 > >832-355-6812 (fax) > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > >Lucas > >Sent: Friday, April 13, 2007 10:41 AM > >To: histonet@pathology.swmed.edu > >Subject: [Histonet] Proper Decalcification > > > >I've been having problems with my PA when it comes to proper > >decalcification of our bone specimens. They are either over or under > >decalcified. > > > >Do many of you use a chemical end point method to determine the > >decalcification or do you use other methods? If so, could you tell me > >the best product to use? > > > >I appreciate any feedback. > > > >Paula Lucas > >Lab Manger > >Bio-Path Medical Group > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Fri Apr 27 11:23:16 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Apr 27 11:23:19 2007 Subject: [Histonet] Formalin substitutes In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF012475FE@wahtntex2.waht.swest.nhs.uk> Message-ID: <115952.80598.qm@web55805.mail.re3.yahoo.com> Right. Right idea, wrong king. So much for being smart at 11pm. --- Kemlo Rogerson wrote: > Getting pathologists to change seems to be in the > same league as Xerxes > telling the tide to stop. > Rene > > Xerxes (the Great) was a Persian Emperor who > attempted to bridge the > Hellespont and whipped (300x) it when a storm > destroyed his bridges; his > second attempt to bridge the strait was successful. > > King Canute (some 1800 years later) sat on the > beach, on his thrown, and > tried to stop the tide. So I guess your comparison > is historically > inaccurate IMHO. > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > I want it said of me by those who knew me best, that > I always plucked a > thistle and planted a flower where I thought a > flower would grow. > --Abraham Lincoln > > This e-mail is confidential and privileged. If you > are not the intended > recipient please accept my apologies; please do not > disclose, copy or > distribute information in this e-mail or take any > action in reliance on > its contents: to do so is strictly prohibited and > may be unlawful. > Please inform me that this message has gone astray > before deleting it. > Thank you for your co-operation > > > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From lpwenk <@t> sbcglobal.net Fri Apr 27 18:48:23 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 27 18:49:50 2007 Subject: [Histonet] ? why does my gallbladder control not stain for bile In-Reply-To: <002c01c788da$b7c222f0$92003712@mit.edu> Message-ID: <001301c78926$8b888110$c0a42e4b@HPPav2> I think I can explain some of it, but I'd love to pathologist's input into the exact type of "bile" in the wall of the gallbladder. I'm assuming you are doing the Fouchet reaction, using trichloractic acid and ferric chloride. This reaction take bilirubin (which is a brown-yellow color) and oxidizes it back biliverdin (which is a green color). When red blood cells die, they are broken down in the spleen and bone marrow to heme and globin. The globin is further broken down to amino acids and are recycled by the body into new proteins. The heme part is broken down into iron and biliverdin. The iron is sent back to the bone marrow to make new RBC's. This leave the biliverdin, which the macrophages turn into water-insoluble unconjugated bilirubin. When this insoluble bilirubin arrives in the liver, glucuronic acid is added, make it a water-soluble conjugated bilirubin. The bilirubins are brown-yellow in color. The bile that is found in gallbladder is a mixture of conjugated and unconjugated bilirubin, with some biliverdin (and salts and cholesterol). So when doing the Fouchet stain, the brown-yellow bilirubin that in trapped in the liver due to cirrhosis or obstruction is converted back to green biliverdin (with a van Gieson counterstain). Our control for the Fouchet stain is a liver with bile duct obstruction. I've never tried this stain on a gall bladder. So, this is the part I'm not sure about, and where I could use a pathologist's input - why is the gallbladder wall green? I'm supposing that it is because it has absorbed biliverdin, not the bilirubin. If that is the case, then the Fouchet stain won't work, because there is no bilirubin to convert back to biliverdin. I'd love to hear other explanations, theories, conjectures, etc. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy Cormier Sent: Friday, April 27, 2007 10:46 AM To: histonet@pathology.swmed.edu Subject: [Histonet] ? why does my gallbladder control not stain for bile Hello All, Why does my gallbladder control not stain for bile? ( the mouse livers do not stain either, but that is not unexpected ) It is freshly cut, the solutions are commercially bought and appropriately stored, the stain seems very straightforward... Shouldn't this stain the bile in the gallbladder? I can see it in the block... all nice dark green...Am I just missing the obvious? Am I clueless? (Don't answer that!) I did this stain like, one other time, in the early 90's and we had a nice piece of liver w/ bile for a control. I have read Carson's and Bancroft's books, and they say the best control is a piece of tissue w/ bile, so the gallbladder should work, right? Help! (and thanks!) Kathy Div Comp Medicine MIT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sat Apr 28 07:00:23 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Apr 28 07:01:53 2007 Subject: [Histonet] NSH Symposium Message-ID: <000301c7898c$cde4a910$da022e4b@HPPav2> I just looked at the NSH website, and the workshop and lecture program for the Oct. 26-31, 2007 NSH Symposium in Denver, Colorado is posted. Note under the registration page says the registration programs will be mailed the last week of April, and will be available on-line the first week of May. If you want a sneak preview of the program, go to http://www.nsh.org Highlight "Symposium/Convention" on the left side. Click on "Educational Sessions". Some of the other links under Symposium/Convention also have information, some don't yet. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From tahseen <@t> brain.net.pk Sat Apr 28 07:19:54 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Sat Apr 28 07:23:50 2007 Subject: [Histonet] Cost analysis Message-ID: <006c01c7898f$8910b290$951480cb@piii> Dear All, I am currently working on a cost analysis for Muscle/ Liver Biopsy & frosen section. Does anyone know the" average"Consultant time per slide or per case on light microscopy. Any information would be appreciated. Thanks advance. Muhammad Tahseen Histology Supervisor, Shaukat Khanum Memorial Cancer Hospital & Research Centre Lahore,Pakistan. tahseen@brain.net.pk From rjbuesa <@t> yahoo.com Sat Apr 28 07:36:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 28 07:36:25 2007 Subject: [Histonet] Cost analysis In-Reply-To: <006c01c7898f$8910b290$951480cb@piii> Message-ID: <705512.18706.qm@web61219.mail.yahoo.com> Tahseen: 1- liver (or other Bx) from sample to slide: $2.00 materials + $2.71 labor = $4.71 2-FS: $0.70 materials + $4.84 labor - $5.54 Under separate cover I am sending you a "pdf" of an article of mine on the sibject (Advance MLP 15 Jan./07) Ren? J. Tahseen wrote: Dear All, I am currently working on a cost analysis for Muscle/ Liver Biopsy & frosen section. Does anyone know the" average"Consultant time per slide or per case on light microscopy. Any information would be appreciated. Thanks advance. Muhammad Tahseen Histology Supervisor, Shaukat Khanum Memorial Cancer Hospital & Research Centre Lahore,Pakistan. tahseen@brain.net.pk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From Eric <@t> ategra.com Fri Apr 27 16:57:05 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sat Apr 28 12:34:27 2007 Subject: [Histonet] Histology jobs both temp and perm, can you help? Message-ID: Hi - Histonetters -Are you still working at ? May I call you at or to discuss jobs? I have both permanent and temporary J0BS for HistoTechs throughout the US. If any of these jobs are not in an the City or State you are or would like to be. Please call me or send me an e-mail and I will look in that specific area for you! ----------------------------------- Temporary HISTOTECH J0BS : ----------------------------------- (Updated APR 27 2007) 1. South Carolina - Bench - 3 months (starting in August) -Interviewing immediately-filled 2. New Hampshire - 3 months - Routine Histology-filled 3. California - (San diego area) - 2 months -Routine Histology- (Start in July)- STILL AVAILABLE........ ------------------------------------------------------- New Permanent HISTOTECHJ0BS Listed below ------------------------------------------------------- (Updated APR 27 2007) 1. Central Florida -Histotech-Bench-perm (BrandNew, HOT) 2. Florida( Tampa Bay area) Histotech- bench- perm 3. Virginia (Central)- Histotech- perm -bench- BrandNew 4. Virginia (Close to North Carolina Border!!) bench- perm 5. Florida ( Tampa Bay area) bench- perm- BrandNew, HOT) 6. Oregon - perm- bench- HOT 7. West Virginia - perm- bench 8. Massachusetts ( Greater Boston Area) Histotech Supervisor 9. Massachusetts (Greater Boston Area) Histotech- Bench - perm 10. Northern Ohio- Histotech - bench - perm 11. Virginia (Close to North Carolina Border!!) bench- perm -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 223 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From marybeth.kaulahao <@t> thibodaux.com Sat Apr 28 17:56:24 2007 From: marybeth.kaulahao <@t> thibodaux.com (Marybeth Kaulahao) Date: Sat Apr 28 17:56:27 2007 Subject: [Histonet] AFB Contamination References: <4BC300747AF87A48BCDF8E48BC2885CE040BB9C3@mail1.calgary.com> Message-ID: <012a01c789e8$722d0700$7496010a@trmc.thibodaux.com> We have been getting false positives as well. The pathologists have decided it is the water. Now we use distilled water in the float bath and to rinse the slides. It seems to work so far. mb ----- Original Message ----- From: To: Sent: Tuesday, April 24, 2007 3:13 PM Subject: [Histonet] AFB Contamination Hi, We are experiencing transient false positive ZN staining on skin biopies. Today a slide had clumped AFB over the majority of the slide where no tissue was present. Previous to this, only one or two bacilli were noted on the slide. Has anyone investigated and found a contaminated waterbath or perhaps the Kinyoun's is the culprit as our lab reuses both? Any help would be appreciated. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Sun Apr 29 12:06:47 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Apr 29 12:06:53 2007 Subject: [Histonet] CD3 clean with RB monos Message-ID: <042920071706.6961.4634D0A70002B0A400001B3122007348409D09020704040A0105@comcast.net> Marvin, thanks for this tremendous reply. I hope many have copied it for you have provided a wealth of background information that goes to core of why IHC even works in the first place. I still have an uneasy feeling when I hear or read advertisements from companies regaling nano to pico molar potential improvements. My sense of theory is suddenly shocked until I can think this through. At that 25 square angstrom or so (or 3,000 cubic angstrom or so), whichever kind of figure you want to use, the 4 non-covalent forces of hydrogen bonding, electrostatic interactions, hydrophobic interactions and Van der Waals dispersion interactions, rule the day and determine strength between the binding "pocket" and its binding partner. But amino acids are amino acids whether from mouse or rabbit. The degrees of freedom of the carbons and/or nitrogen in the amino acid chain are set not by whether it is mouse or rabbit. All chemistry (of the atoms themselves) is the same for mouse or rabbit. So whi le things might certainly be different between mouse and rabbit (accessory molecules or amino acid distributions or breaking of immunological tolerance or somatic hypermuation differences or anything like that) I just can't see how the binding sites, which affect the strength of the binding, can be up to 1,000 fold different. But if there are immunological type chemists out there who can explain it to the Histonet, I'd be super happy to hear from you. Thanks again Marvin, Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: Marvin Hanna > Hi Ray, > > Thanks for the additional input. I stand corrected. Herceptin is a > HUMANIZED mouse monoclonal antibody. From Dr. Kimball's online > Biology textbook, "The [Humanized] antibody combines only the amino > acids responsible for making the antigen binding site (the > hypervariable regions) of a mouse (or rat) antibody with the rest of > a human antibody molecule thus replacing its own hypervariable > regions." This helps reduce the problem of HAMA (human anti-mouse > antibodies), which can cause damage to the kidneys and cause the > therapeutic antibodies to be quickly eliminated from the human patient. > > http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/ > Monoclonals.html > > I will also qualify my statement, "with up to a 10 times greater > affinity" with "according to a number of IHC vendors of rabbit > monoclonal antibodies". I have found at least 4 making claims of > higher affinity. The Epitomics website claims that mouse monoclonals > have affinities of "Nanomolar (~10-9 KD M)" and rabbit monoclonals > have affinities of "Picomolar (10-12 KD M) possible", a thousand fold > potential increase. I did not know that there are researchers who > disagree with these statements and appreciate you pointing it out. I > agree additional research should be done. > > http://www.epitomics.com/technology/tech.html > > According to a news release on the Epitomics website, they are > providing humanized antibodies for therapeutic research. It is early > to speculate that (possible) higher affinities of humanized rabbit > monoclonals will provide a better therapeutic response in patients > compared to humanized mouse monoclonals and will require many years > of research and clinical trials to determine. > > I also agree I have used rabbit monoclonal antibodies in IHC that > have not given better results, and sometimes worse results, than > their mouse monoclonal counterparts. > > Best Regards, > > Marvin Hanna > mhanna@histosearch.com > > > On Apr 23, 2007, at 4:32 PM, koellingr@comcast.net wrote: > > > PS. > > As far as I know Herceptin (trastuzumab) is a HUMANIZED monoclonal > > antibody and not a mouse or rabbit monoclonal. While certainly > > many things are possible, I'm skeptical of rabbit monoclonals, no > > matter how great they are for IHC, going into human therapeutics in > > light of the norm which is to humanize these reagents. Unless you > > were to "humanize" the rabbit monoclonal itself similar to > > humanizing mouse monoclonals. > > > > Ray Koelling > > Phenopath Laboratories > > Seattle, WA > > -------------- Original message ---------------------- > > From: koellingr@comcast.net > >> Rena, > >> > >> I agree that the epitomics website is great for learning about > >> this technology > >> and I further agree it could be of help as has been mentioned > >> before. I am a > >> bit skeptical of claims of a 10-fold better affinity than mouse > >> monoclonals as a > >> blanket statement. > >> > >> At a previous meeting a while back we asked about claims of higher > >> affinity and > >> couldn't get a sufficient (to our mind) response. To me that > >> response would be > >> in the form of a side to side comparison, using the same > >> immunogen, and with a > >> host mouse and rabbit side by side each producing monoclonals to > >> same target to > >> see which might be better. I believe the concept of a superior > >> rabbit > >> monoclonal technology in terms of being able to break > >> immunological tolerance in > >> the immunized systems, especially for some difficult targets, is > >> probably right > >> on. > >> But simply breaking tolerance is not the same as producing higher > >> affinity > >> antibodies. > >> > >> For the blanket statement that rabbit monoclonals have higher > >> affinity, I'd like > >> to see data comparing them to their (identical) target in a mouse > >> host and see > >> data such as from Biacore, x-ray crystallography and that > >> differences in somatic > >> hypermutation are indeed making these higher affinity. > >> That being said, I agree that the rabbit monoclonals have great > >> use and even > >> more potential. I've used several (many) that are far superior to > >> their > >> counter-part mouse monoclonals. However, I've used (and heard of) > >> a few that > >> weren't as good. > >> So while I like rabbit monoclonals, use them, advocate for them > >> and endorse the > >> suggestion you try them, I'm not convinced that as a rule they > >> have 10x higher > >> affinity than do mouse monoclonals. > >> > >> Ray Koelling > >> Phenopath Laboratories > >> Seattle, WA > >> > >> -------------- Original message ---------------------- > >> From: "Mildred Fail" > >>> We have had quite a problem with CD3s on bone marrow biopsies being > >>> "messy" both with mouse monoclonals and rabbit polyclonals. Protein > >>> block is used. Diluting the Ab out further lost some cells in the > >>> lymph > >>> node control. We tried a rabbit monoclonal. The staining is very > >>> specific and intense. The slide is beautifully free of extraneous > >>> staining. The higher dilution has not appeared to have effected the > >>> number of cells stained. Question is why would the rabbit > >>> monoclonal produce a cleaner slide? > >>> Rena Fail > >>> > >>> Rena Fail > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From relia1 <@t> earthlink.net Sun Apr 29 21:24:50 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Sun Apr 29 21:24:54 2007 Subject: [Histonet] RELIA's Histology Careers Bulletin from Pam Barker 4/30/07 Message-ID: Hi Histonetters, I hope you are enjoying a beautiful Spring! Can you believe May Day is Tuesday!!! This is a quick update of my client?s current openings. As you know all of the positions I work with are full-time permanent positions with the best hospitals, labs and clinics. I only work with clients that offer excellent compensation including competitive salaries, great benefits and relocation assistance /sign on bonuses. These positions are Day Shift M-F unless noted otherwise. I represent companies nationwide that are in need of histology supervisors histotechnologists and histo technicians. Here are my Newest Openings: Lead Histotechnologist ? Greater Boston Area Histo Tech ? Virginia/North Carolina Border Histo Tech ? Los Angeles, CA Here is a list of my most current openings: HISTOLOGY MANAGEMENT: Histology Manager ? Greater Boston Area Histology Manager ? Central Florida IHC Supervisor ? Los Angeles, CA Histology Supervisor ? South Texas (For your coworkers in Cytology I have an opening for a cytology supervisor in PA) HISTOLOGY TECHNICIAN/TECHNOLOGIST Histo Tech ? South Texas Histo Tech ? Rhode Island Histo Tech/PA ? North Central Florida Histo Tech ? Southwest Florida Histo Tech ? Illinois Histo Tech 3rd shift ? OH If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. If you know of anyone who would like to receive my e-mail as well please feel free to send me their contact information and I would be happy to send it to them as well. Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 30 01:24:58 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 30 01:25:05 2007 Subject: [Histonet] AFB Contamination Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247613@wahtntex2.waht.swest.nhs.uk> You get an AAFB in water but I can't remember its name. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 30 01:54:07 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 30 01:54:13 2007 Subject: [Histonet] Formalin substitutes Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247616@wahtntex2.waht.swest.nhs.uk> Xerxes was an emperor not a King. A king recognises that, in religion, the church is an equal or even superior whilst an emperor doesn't. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Mon Apr 30 06:15:36 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Apr 30 06:15:47 2007 Subject: [Histonet] Formalin substitutes Message-ID: <407F05A128805F4C879A33DBA32E618E20C2E1@TRFT-EX01.xRothGen.nhs.uk> Now - that's the first new thing I've learned today. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 30 April 2007 07:54 To: Roger Moretz; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin substitutes Xerxes was an emperor not a King. A king recognises that, in religion, the church is an equal or even superior whilst an emperor doesn't. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rbruggeman <@t> psu.edu Mon Apr 30 09:52:24 2007 From: rbruggeman <@t> psu.edu (Richard Bruggeman) Date: Mon Apr 30 09:53:03 2007 Subject: [Histonet] Dako CD4 on FFPE? Message-ID: <4635CA68.48B8.00C6.0@psu.edu> Has anyone been successful in using Dako's CD4 antibody for IHC on FFPE tissue. I know that Dako does not recommend this antibody for IHC on FFPE tissues, but I was curious if anyone has tried it and gotten it to work. If you were successful in this, could you please provide some protocol details. Thanks in advance. Trey Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 From hej01 <@t> health.state.ny.us Mon Apr 30 10:14:32 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Apr 30 10:14:38 2007 Subject: [Histonet] CD90.2 Message-ID: Hi Histonetters, Is there a vendor that has CD90.2 specifically for IHC - formalin-fixed, paraffin-embedded mouse tissue? Thanks. Helen Johnson (hej01@health.state.ny.us) From rjbuesa <@t> yahoo.com Mon Apr 30 11:00:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 30 11:00:15 2007 Subject: [Histonet] Dako CD4 on FFPE? In-Reply-To: <4635CA68.48B8.00C6.0@psu.edu> Message-ID: <417166.19819.qm@web61221.mail.yahoo.com> Richard: I had to use NovoCastra Ab (1:5, pH8 HIER, tonsil +control w. FFPE tissues) Ren? J. ard Bruggeman wrote: Has anyone been successful in using Dako's CD4 antibody for IHC on FFPE tissue. I know that Dako does not recommend this antibody for IHC on FFPE tissues, but I was curious if anyone has tried it and gotten it to work. If you were successful in this, could you please provide some protocol details. Thanks in advance. J. y Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From gu.lang <@t> gmx.at Mon Apr 30 11:08:12 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 30 11:08:21 2007 Subject: [Histonet] CD4 (4B12) protocol on Benchmark XT Message-ID: <000001c78b41$c146db90$6412a8c0@dielangs.at> Hi, can someone provide me with a successfull protocol for CD4 clone 4B12 for the Benchmark XT? Thanks in advance Gudrun From Valerie.Hannen <@t> parrishmed.com Mon Apr 30 11:48:17 2007 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Apr 30 11:48:23 2007 Subject: [Histonet] Bone Marrow Biopsies Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34AD@ISMAIL.parrishmed.local> Kristen, We fix our BM bx's in zinc formalin for 2 hrs and then decal for 3 hrs. Hope this helps. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida 32796 (321)268-6333 ext.7506 ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From Mark.Frei <@t> sial.com Mon Apr 30 11:56:19 2007 From: Mark.Frei <@t> sial.com (Mark Frei) Date: Mon Apr 30 11:56:43 2007 Subject: [Histonet] Re: Histonet Digest, Vol 41, Issue 47 AFB Contamination In-Reply-To: <200704291712.l3THCgtA004808@stlspfw2.sial.com> Message-ID: Carbol-Fuchsin has been known to precipitate out of solution, usually due to exposure to extreme temperatures during shipping. The small red granules can be confused with AFB. A clue would be seeing dye particles deposited on parts of the slide where there should be no specimen. Pitch the bottle and ask for a replacement. Do not try to filter! Mark Frei MT(ASCP) From gu.lang <@t> gmx.at Mon Apr 30 14:55:37 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 30 14:55:45 2007 Subject: AW: [Histonet] CD4 (4B12) protocol on Benchmark XT In-Reply-To: Message-ID: <001401c78b61$863cd250$6412a8c0@dielangs.at> What are the specifications of your protocol? CC1 mild or standard Antibody-dilution? I use the ultra-view polymer kit; CD4 (4B12) from Novocastra. Best regards Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _____ Von: Vivian Anderson [mailto:vivanderson14@hotmail.com] Gesendet: Montag, 30. April 2007 19:53 An: gu.lang@gmx.at Betreff: RE: [Histonet] CD4 (4B12) protocol on Benchmark XT Hi, I have a protocol for C4d on Ventana Benchmark. It works great! I deparaffinize on line and use CC1 Standard. Incubate for 26 minutes. Amplify Use A/B Block and counterstain. Good luck! Vivian Anderson, HT(ASCP) Lead Histotech Memorial Medical Center Springfield, Illinois _____ From: "Gudrun Lang" Reply-To: gu.lang@gmx.at To: Subject: [Histonet] CD4 (4B12) protocol on Benchmark XT Date: Mon, 30 Apr 2007 18:08:12 +0200 Hi, can someone provide me with a successfull protocol for CD4 clone 4B12 for the Benchmark XT? Thanks in advance Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ MSN is giving away a trip to Vegas to see Elton John. Enter to win today. From carl.hobbs <@t> kcl.ac.uk Mon Apr 30 15:00:20 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Apr 30 15:00:49 2007 Subject: [Histonet] Re: Emperor Message-ID: <002601c78b62$2eca1540$4001a8c0@carlba65530bda> Shoot me down, lol. There was no such word as "Emperor" in Xerxes' time: perhaps "King of Kings"? Imperator/Emperor is Roman . Also, even Xerxes paid homage to the Gods......;-) Too dangerous not to...... PS: I really larf at Napoleon's insolence when he crowned himself "Emperor".....and he forced The Church to attende....but not to officiate. OK: I will Immuno-orient this: which immunodetection system is Emperor? Or do we have such systems that are Kings for a day? From cindipqr <@t> wowway.com Mon Apr 30 18:22:50 2007 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Mon Apr 30 17:22:54 2007 Subject: [Histonet] Neurogenin1 on mouse or rat tissue Message-ID: <20070430222250.M98121@wowway.com> Hi All, Does anyone know of a Neurogenin-1 antibody that works on FFPE mouse and rat tissue? Thanks, Cindi Roberts Neurology Research HFH Detroit, Mi.