From kappeler <@t> patho.unibe.ch Fri Sep 1 06:14:44 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Fri Sep 1 06:15:25 2006 Subject: [Histonet] Blood group antigens: antibodies for IHC on FFPE tissue Message-ID: <00a701c6cdb7$e634ff60$27955c82@patho.unibe.ch> We used to work with monoclonal Abs from Dako against blood group antigens A (clone 81FR2.2), B (clone 3E7), and H (clone 92FR-A2). Unfortunately, Dako has discontinued these products. Does anybody have a source for such antibodies (not necessarily same clones) that work reliably on FFPE human tissues? Many thanks! Andi Kappeler Institute of Pathology, University of Bern, Switzerland From msviapiano <@t> yahoo.com Fri Sep 1 08:19:04 2006 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Fri Sep 1 08:19:12 2006 Subject: [Histonet] Microscopes: Thanks for all the info! Message-ID: <20060901131904.11796.qmail@web54511.mail.yahoo.com> Hello everybody, Thank you very much for all the good advice and interesting links that I got regarding the basic microscopes for my lab. I will post here additional questions once I learn more about the options that I have for buying. Just for additional information, my major purchase is likely going to be a Zeiss Axiovert-200 for fluorescent live-cell imaging. I need an additional basic inverted scope for routine checking of live and stained cells and tissue slices (no high mag here), and a relatively simple stereomicroscope for tissue microdissection. I have found that there are two forgotten microscopes in my department, which I can freely "share" (i.e., install) in my new lab: An inverted Nikon Diaphot (with plan-achro objectives from 4x to 40x) and a stereo Nikon SMZ-10. However, both seem to have been through a minor war or something: they were kept in a warm/humid room used for cultures and I don?t know how badly they may be affected. In addition, the Diaphot doesn?t have a specimen carrier so I will need to contact Nikon and see about accessories for that scope. Best regards to all, Mariano Mariano S. Viapiano, PhD Center for Molecular Neurobiology Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Fri Sep 1 09:26:19 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 1 09:26:32 2006 Subject: [Histonet] OCT embedded Tissue Storage In-Reply-To: <20060831185235.32327.qmail@web50914.mail.yahoo.com> References: <20060831165028.65482.qmail@web61214.mail.yahoo.com> <20060831185235.32327.qmail@web50914.mail.yahoo.com> Message-ID: <6.0.0.22.1.20060901082116.01b4f4f0@gemini.msu.montana.edu> >One of the clever suggestions, made by Patsy Ruegg years ago. Place your >resealed (and foil wrapped if you wish) OCT block in a labelled tissue >cassette, store in boxes suggested. It is also important to keep a freezer log of what you horde From BMolinari <@t> heart.thi.tmc.edu Fri Sep 1 09:34:15 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 1 09:34:19 2006 Subject: [Histonet] B5 Immuno Message-ID: Happy Friday, An investigator asked me about this and having very little experience with immunos I come to you. She found an article where myocardial sections were fixed in B5 without formalin (is there a B5 without formalin?) and then stained with several polyclonal and monoclonal antibodies. She wanted to know why B5? Wouldn't 10% NBF be just as effective? I did some archiving and read that some have had success with zinc formalin and B Plus. Also I do know of the hazards of B5 so we will not be using it in my lab, but just wondering. Thanks, Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From Don.Birgerson <@t> leica-microsystems.com Fri Sep 1 10:24:21 2006 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Fri Sep 1 10:24:33 2006 Subject: [Histonet] Parts for Laborlux 12 In-Reply-To: <44F6F002.2020003@qmul.ac.uk> Message-ID: Hi Mike, Since "Leitz-Wetzler" is a Leica Microscope, I suggest you try the web www.Leica-Microsystems.com The web site will give you the chance to ask your question and refer the question to our UK office for a more localized answer. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 Michael Doube To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Parts for Laborlux 12 08/31/2006 09:19 AM Hi All I acquired a Leitz-Wetzlar Laborlux 12 microscope with fluorescence attachments during a recent pathology lab clear-out. It's a bit old but in good condition, so I am renovating it. Does anyone have a trinocular head, with C mount or SLR camera (esp. Canon) that would be suitable for this unit, that they are willing to part with or swap for the binocular head? Know anyone who might? Thanks, Mike -- Michael Doube BPhil BVSc MRCVS PhD Student Dental Institute Queen Mary, University of London New Rd London E1 1BB United Kingdom Phone +44 (0)20 7377 7000 ext 2681 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sbelma <@t> lsuhsc.edu Fri Sep 1 16:13:09 2006 From: sbelma <@t> lsuhsc.edu (Belmadani, Souad) Date: Fri Sep 1 16:13:14 2006 Subject: [Histonet] (no subject) Message-ID: <28F2770D73F1B7438869424D8019A89E124878@EXCHBE2.master.lsuhsc.edu> Hi, im trying to stain endothelium in mice hearts, i used the anti CD31 from abcm and it did not work , now im using the FVW from DAKo again its not working. im using the antibody at 1;200 dilution overnight and no staining at all its formalin paraffine section and im using the citrate buffer ph 6 as antigen retrieval .... any ideas why its not working i apprecite your help thanks souad From Vivian.King <@t> CLS.ab.ca Fri Sep 1 16:16:46 2006 From: Vivian.King <@t> CLS.ab.ca (Vivian.King@CLS.ab.ca) Date: Fri Sep 1 16:16:54 2006 Subject: [Histonet] Alcian Blue 8GX Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE1BC517@mail1.calgary.com> Is there any difference between Alcian Blue 8GX (Ingrain Blue 1) and just plain Alcian Blue 8GX? And do they work the same way in staining methods? CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From rjbuesa <@t> yahoo.com Sat Sep 2 08:58:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Sep 2 08:58:24 2006 Subject: [Histonet] (no subject) In-Reply-To: <28F2770D73F1B7438869424D8019A89E124878@EXCHBE2.master.lsuhsc.edu> Message-ID: <20060902135818.57087.qmail@web61220.mail.yahoo.com> Souad: I used CD31 (placenta as + control) from Dako (mouse monoclonal) at 1:20 on formalin fixed paraffin processed tissue and HIER citrate at pH6; primary during 30 minutes. Always with good results. The difference with your protocol is that you dilute more but at the same time you incubate overnight and this should compensate for your 10 times more dilution rate. I don't really see anything wrong with your protocol unless something happens during the overnight incubation; too low temperature perhaps? I would try first a shorter incubation and less dilution. Hope this will help you. Ren? J. "Belmadani, Souad" wrote: Hi, im trying to stain endothelium in mice hearts, i used the anti CD31 from abcm and it did not work , now im using the FVW from DAKo again its not working. im using the antibody at 1;200 dilution overnight and no staining at all its formalin paraffine section and im using the citrate buffer ph 6 as antigen retrieval .... any ideas why its not working i apprecite your help thanks souad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From kemlo <@t> f2s.com Sun Sep 3 04:13:25 2006 From: kemlo <@t> f2s.com (kemlo) Date: Sun Sep 3 04:20:26 2006 Subject: [Histonet] Lyme disease agent staining In-Reply-To: Message-ID: <004101c6cf39$367c5040$0302a8c0@KEMLOS> Rats, dunno, I'll find out. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: 31 August 2006 16:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lyme disease agent staining I have a case which may be Lyme disease, manifesting as erythema chronicum migrans, the first skin manifestation. Has anyone had any experience? If so, what silver stain did you use to demonstrate the organism? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Sep 3 10:19:29 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Sep 3 10:20:07 2006 Subject: [Histonet] Parvovirus control slides Message-ID: <44FABA410200007700001C1C@hcnwgwds01.hh.chs> Someone from Canada contacted me a few months ago about getting some unstained slides from tissue infected with Parvovirus. I apologize - I misplaced your name and address. If you still need them please contact me and I will forward the unstained slides to you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From Rcartun <@t> harthosp.org Sun Sep 3 10:36:56 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Sep 3 10:37:48 2006 Subject: [Histonet] L1 (CD171) IHC staining Message-ID: <44FABE580200007700001C26@hcnwgwds01.hh.chs> Is anyone doing immunohistochemical staining for "L1" (CD171) on formalin-fixed, paraffin-embedded tissue? If so, whose antibody are you using? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From gu.lang <@t> gmx.at Sun Sep 3 13:37:33 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Sep 3 13:37:42 2006 Subject: [Histonet] ferrous hematein Message-ID: <001501c6cf88$052c5090$eeeea8c0@SERVER01> Hi all, in the histonet archives I found an entry about masson trichrom from Peggy Wenk. She mentioned ferrous hematein usage instead of Weigerts Hem. Can someone provide me with the recept of this dye? thanks in advance Gudrun Lang From rjbuesa <@t> yahoo.com Sun Sep 3 14:04:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 3 14:04:04 2006 Subject: [Histonet] ferrous hematein In-Reply-To: <001501c6cf88$052c5090$eeeea8c0@SERVER01> Message-ID: <20060903190401.5088.qmail@web61223.mail.yahoo.com> Gudrun: Is you are referring to "ferrous sulfate" as ingredient of Lillie and Earle's hematein (hematoxylin) I can send you the recipe. Ren? J. Gudrun Lang wrote: Hi all, in the histonet archives I found an entry about masson trichrom from Peggy Wenk. She mentioned ferrous hematein usage instead of Weigerts Hem. Can someone provide me with the recept of this dye? thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From fferreir <@t> ibmc.up.pt Mon Sep 4 05:06:55 2006 From: fferreir <@t> ibmc.up.pt (=?iso-8859-1?b?RuF0aW1h?= Ferreirinha) Date: Mon Sep 4 05:33:11 2006 Subject: [Histonet] Co-localization studies... Message-ID: <1157364415.44fbfabf6d559@webmail.ibmc.up.pt> Hello Histonetters, I am a little bit ashamed, but I have a very basic question to put you: when we are doing co-localization studies (IF) both primary antibodies should not be raised in the same species. Why? What would be the result? I would like to read a little bit more about this subject, where should I look for? Thank you, F?tima -- F?tima Ferreirinha IBMC R. do Campo Alegre, 823 4150-180, Porto Portugal ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From histosci <@t> shentel.net Mon Sep 4 14:15:25 2006 From: histosci <@t> shentel.net (HSRL) Date: Mon Sep 4 14:13:43 2006 Subject: [Histonet] HSRL Job Opportunities Message-ID: <000701c6d056$7d12b260$0700a8c0@HSRLMAIN> Dear Netters, Histo-Scientific Research Laboratories (HSRL, Inc.) is looking to add two full time histotechs and one part time histotech to our team. HSRL is a privately-owned histopathology laboratory located in the Shenandoah Valley of Virginia. We are about 1 ? hours SW of Washington DC tucked away in the beautiful Blue Ridge Mountains. The ideal candidate would have skills in basic paraffin histology as well as plastics (GMA and/or MMA) histology. We offer a full benefits package along with competitive wages, paid vacation days, and a FRIENDLY and RELAXED ATMOSPHERE! If you are interested in learning more about the opportunities at HSRL, please fax or e-mail resume to: Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 FAX: 540.477.4448 tomgalati@hsrl.org www.hsrl.org Thank you. From TJJ <@t> Stowers-Institute.org Mon Sep 4 18:14:44 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Sep 4 18:15:10 2006 Subject: [Histonet] RE: Co-localization studies... References: Message-ID: >> I am a little bit ashamed, but I have a very basic question to put you: when we are doing co-localization studies (IF) both primary antibodies should not be raised in the same species. Why? What would be the result? I would like to read a little bit more about this subject, where should I look for? Thank you, F?tima<< Fatima - you can do this easily if you have abundant protein and directly labeled antibodies. However, if you are using unlabeled primary antibodies, you need a secondary antibody to complex with the first which adds either an enzyme, biotin, or a fluorescent label. The secondary antibody is usually targeted against the animal species the first antibody was made in. Therefore, if both your primary antibodies are rabbit, and you use a green fluorescent labeled anti-rabbit and a red fluorescent labeled anti-rabbit, both labels will bind with the rabbit species. Everywhere both antibodies bound with the target protein will label with both red and green. Even though that will show colocalization, it does not give you the option of seeing each color channel separately. What if your antigens do not colocalize in every cell? It will look as though it does by doing your immunostaining this way. If primary antibody #1 is located in cell type A and cell type C, and primary antibody #2 is located in cell type B and cell type C, they should only co-localize in C. But by doing it with your method, you will also show colocalization in cell types A, B, and C. Does this make sense? If you use two primary antibodies raised in different species, say goat for Antibody #1 and rabbit for Antibody #2, you can use an anti-goat green fluorescent labeled secondary antibody, and an anti-rabbit red fluorescent labeled secondary antibody. The anti-goat should localize your Antibody #1, and the anti-rabbit should localize your Antibody #2. Therefore, given the above example, Antibody #1 will show positivity in cell type A and C, Antibody #2 will show positivity in cell type B and C, and they will only co-localize in cell type C. For more information on multiple immunostaining methods, I urge you to purchase and read (cover to cover) Chris van der Loos' book, ISBN 038791594X. Best wishes, Teri Johnson Stowers Institute for Medical Research Kansas City, MO From cpomajzl <@t> cpllabs.com Tue Sep 5 06:34:00 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Tue Sep 5 06:27:34 2006 Subject: [Histonet] Sakura Coverslipper Timing Message-ID: <001a01c6d0df$2f491180$26fca8c0@CSP> Histonetters: We have 2 Sakura coverslippers that are in desperate need of some TLC, maintenance and repair. They are 7+ years old, and have been used and abused for all of those years. They are shared equipment between Histo and Cytology. Therefore, there has been 7 years of PAP juice buildup. Our in-house BioMed tech retired a couple of years ago, and ever since, we have used a local BioMed company that does not have much experience with these machines. They would come out and temporarily fix any problems, but it would only correct the problem for a week or two. As a result I have to do much of the maintenance and repair myself. I am somewhat mechanically inclined, but I have my limits. I finally disassembled one of the machines last week so that I could remove all of the disgusting build-up that was covering all of the parts. I was able to put it back together, and it appears to be in good working order, except for one problem. I cannot get the timing of the solenoid in sync with the lever arm that allows the receiving basket to drop one notch of the basket at a time. The service manual explains how to fix the timing, but I don't know that I understand these directions clearly. It just doesn't make much sense, and I simply am not sure I am doing it correctly. Does anyone have any experience who could offer some tips and advice? It would be much appreciated. We are in the process of replacing one of these machines, but that will take some time, I'm sure. And that still leaves me with one old machine. Chris Pomajzl, BS, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. From sjchtascp <@t> yahoo.com Tue Sep 5 08:41:42 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Sep 5 08:41:49 2006 Subject: [Histonet] lighted floatation Bath Message-ID: <20060905134142.5601.qmail@web38209.mail.mud.yahoo.com> I'm looking for a working lighted floatation bath as a primary for our lab. --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From kgrobert <@t> rci.rutgers.edu Tue Sep 5 08:52:37 2006 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Tue Sep 5 08:50:42 2006 Subject: [Histonet] lighted floatation Bath In-Reply-To: <20060905134142.5601.qmail@web38209.mail.mud.yahoo.com> References: <20060905134142.5601.qmail@web38209.mail.mud.yahoo.com> Message-ID: <44FD8125.3020403@rci.rutgers.edu> I like this one from Fisher Scientific: https://www1.fishersci.com/Coupon?cid=1328&gid=137998 Kathleen Roberts Neurotoxicology Labs Rutgers University Steven Coakley wrote: >I'm looking for a working lighted floatation bath as a primary for our lab. > >--------------------------------- >How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From algranth <@t> u.arizona.edu Tue Sep 5 08:58:10 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Sep 5 08:58:19 2006 Subject: [Histonet] Phoenix Message-ID: <4.3.2.7.2.20060905065316.00c7f678@algranth.inbox.email.arizona.edu> This week many of you will be traveling to Phoenix for the 32nd NSH Symposium/Convention and the Arizona Committee is anxiously awaiting for your arrival. We hope that you have a wonderful visit to Phoenix and Arizona. We have ordered cooler temperatures for you and maybe a bit of late summer monsoons. Please stop by the Phoenix PR table in the Convention Center to say hello. Safe travels! ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From micro <@t> superlink.net Tue Sep 5 09:03:51 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Tue Sep 5 09:04:03 2006 Subject: [Histonet] lighted floatation Bath References: <20060905134142.5601.qmail@web38209.mail.mud.yahoo.com> Message-ID: <082d01c6d0f4$1e1b2190$26893cd1@DJ4VDH31> Several available, also other histo and EM equipment. Please contact me off list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 ----- Original Message ----- From: "Steven Coakley" To: Sent: Tuesday, September 05, 2006 9:41 AM Subject: [Histonet] lighted floatation Bath > I'm looking for a working lighted floatation bath as a primary for our > lab. > > --------------------------------- > How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call > rates. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jqb7 <@t> cdc.gov Tue Sep 5 08:56:56 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Tue Sep 5 09:05:26 2006 Subject: [Histonet] lighted floatation Bath Message-ID: I LOVE the one Surgipath sells. It doesn't have the glass insert so it heats very rapidly and has a black background. You want the square one, not the round one. It's product number 04535S. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, September 05, 2006 9:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] lighted floatation Bath I'm looking for a working lighted floatation bath as a primary for our lab. --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DavidH <@t> marketlabinc.com Tue Sep 5 09:06:47 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Tue Sep 5 09:06:56 2006 Subject: [Histonet] lighted floatation Bath Message-ID: <19E3602A16438E48B51A4250CA04B5F680AE9B@exchange.marketlab.com> Barnstead has a much newer model with LED temperature readout, separate light and power switches and a temp probe for less money. http://www.barnsteadthermolyne.com/search_modelnbr.cfm Product Group:Histology Product Category:TISSUE FLOAT BATH Model Number: 26106Q You have to plug the model number into the search field to see the product. Dave Haagsma -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Roberts Sent: Tuesday, September 05, 2006 9:53 AM To: Steven Coakley Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] lighted floatation Bath I like this one from Fisher Scientific: https://www1.fishersci.com/Coupon?cid=1328&gid=137998 Kathleen Roberts Neurotoxicology Labs Rutgers University Steven Coakley wrote: >I'm looking for a working lighted floatation bath as a primary for our lab. > >--------------------------------- >How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From antyler <@t> uncg.edu Tue Sep 5 10:41:11 2006 From: antyler <@t> uncg.edu (Amber Tyler ANTYLER) Date: Tue Sep 5 10:41:20 2006 Subject: [Histonet] Anyone looking for an Ultra Low Freezer Message-ID: Just posting to tell everyone that New Brunswick Scientific has a discount offer on Ultralow freezers. I just received a quote today for half price. Thank you, Amber N. Tyler, Ph.D. Research Scientist Dept of Psychology (PO Box 26170) 296 Eberhart Building, Walker Avenue University of North Carolina - Greensboro Greensboro, NC 27402 From MSHERWOOD <@t> PARTNERS.ORG Tue Sep 5 10:54:14 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Sep 5 10:54:26 2006 Subject: [Histonet] FW: Re: Collagen stain for paraffin sections Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30BC3@PHSXMB1.partners.org> To all: Does anyone know if the collagen stain, Masson Trichrome, would deliniate denatured or necrotic collagen from normal collagen in paraffin sections? One of the researchers is looking for such a stain. If not, is there another collagen stain that would? (We routinely use MTC). Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org From pmarcum <@t> vet.upenn.edu Tue Sep 5 11:15:25 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Sep 5 11:15:37 2006 Subject: [Histonet] If You Can't Go to NSH in AZ We Have an Alternative in October Message-ID: <6.1.1.1.2.20060905115941.01a24ab8@mail.vet.upenn.edu> The Pennsylvania Histotechnology Society is holding an October seminar program in the Pittsburgh area. If you could not go to the NSH Symposium in Phoenix and you will still need to complete CEUs this year join us for the meeting. We will have 3 days of special seminars covering management issues, beginning and advanced IHC classes, forensics autopsy and entomology to name a few. Go to our web site at www.pahisto.org for a full program and registration forms. The meeting will be in Cranberry, PA just outside Pittsburgh so we encourage members of NSH, Ohio, New York, West Virginia and all of Region II to join us in an educational/fun experience. We will have vendor exhibits and a special workshop on automated special stainers they will participate in. We have workshops and talks planned for all phases of Histology and many related fields. We will have a talk on FNA procedures for cytology/histology. We will have safety and autopsy workshops for PAs and others who will be required to have CEUs in these areas. Plese contact me if you have any questions. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From lhartman18 <@t> adelphia.net Tue Sep 5 11:25:40 2006 From: lhartman18 <@t> adelphia.net (lhartman18@adelphia.net) Date: Tue Sep 5 11:25:46 2006 Subject: [Histonet] Peloris tissue processor Message-ID: <6769591.1157473540991.JavaMail.root@web18> Would you please share your experiences with the Peloris tissue processor by visionbiosystems? Any comments would be appreciated. Thanks, Linda Hartman Histology Section Head Mount Nittany Medical Center State College, PA 16803 814-234-6117 X6650 email lhartman@mountnittany.org From Vickroy.Jim <@t> mhsil.com Tue Sep 5 11:48:40 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Sep 5 11:48:49 2006 Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR Message-ID: Are there folks supervising histology laboratories that only have an HT certification and not a degree? And............. What do the governing organizations say about this? I know I can get the information but wondered if anyone had a quick answer. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From doug <@t> ppspath.com Tue Sep 5 11:56:11 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 5 11:53:20 2006 Subject: [Histonet] Peloris tissue processor In-Reply-To: <6769591.1157473540991.JavaMail.root@web18> Message-ID: Run away! Ask them for a list of users. I have one sitting in my storage area waiting on them to pick it up. It has never worked right and the tech support is non existent. The techs do not know how to fix it. It is the second one that they have sent us. If anyone from Vision is reading this please have it removed from our storage area. It has been here for four months waiting on you to pick it up. I hope the acquisition of this company doesn't tarnish the Ventana reputation. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lhartman18@adelphia.net Sent: Tuesday, September 05, 2006 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Would you please share your experiences with the Peloris tissue processor by visionbiosystems? Any comments would be appreciated. Thanks, Linda Hartman Histology Section Head Mount Nittany Medical Center State College, PA 16803 814-234-6117 X6650 email lhartman@mountnittany.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Sep 5 12:04:27 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Sep 5 12:04:43 2006 Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D95@EMAIL.archildrens.org> I do not have a degree. I took the HTL through a grandfather clause. It is my understanding the pathologist is considered the actual supervisor of histology (because they are the ones reporting results) and he/she has the authority to delegate the responsibilities. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 05, 2006 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR Are there folks supervising histology laboratories that only have an HT certification and not a degree? And............. What do the governing organizations say about this? I know I can get the information but wondered if anyone had a quick answer. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Charlotte.Kopczynski <@t> baycare.org Tue Sep 5 12:29:21 2006 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Tue Sep 5 12:29:28 2006 Subject: [Histonet] Histology Openings Message-ID: BayCare Health System, serving the Tampa Bay Area, has openings for: Histotechnicians/Histotechnologists.... Current Florida license and ASCP certification required. Performs routine histology procedures in a fully automated laboratory. Other duties include responsibility for instrumentation operation and maintenance. Record documentation, quality control monitoring, computer applications and lab safety requirements are also needed. Strong interpersonal and excellent customer service skills. Good communication skills. Contact: BayCare Regional Services E-mail: regional.teamresources@baycare.org 16255 BayVista Drive Clearwater, FL 33760 Phone (866) 221-3222 Apply On Line - http://baycare.org Company Profile The Tampa Bay area's leading not-for-profit hospitals, BayCare Health System is a joint operating agreement between three Community Health Alliances (CHAs). Each CHA uniquely focuses on the distinctive needs of their communities and taps into the regional system for improved quality, access and efficiencies. The CHAs are: * St. Anthony's Health Care, which includes St. Anthony's Hospital in St. Petersburg. * Morton Plant Mease Health Care, which includes Morton Plant Hospital in Clearwater and Morton Plant North Bay Hospital in Pasco County. Mease Countryside and Mease Dunedin hospitals. * St. Joseph's-Baptist Health Care, which includes St. Joseph's, St. Joseph's Children's, and St. Joseph's Women's hospitals in Tampa and South Florida Baptist Hospitals in Plant City. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, September 05, 2006 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 34, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. HSRL Job Opportunities (HSRL) 2. RE: Co-localization studies... (Johnson, Teri) 3. Sakura Coverslipper Timing (Chris Pomajzl) 4. lighted floatation Bath (Steven Coakley) 5. Re: lighted floatation Bath (Kathleen Roberts) 6. Phoenix (Andrea Grantham) 7. Re: lighted floatation Bath (Markus F. Meyenhofer) 8. RE: lighted floatation Bath (Bartlett, Jeanine (CDC/CCID/NCID)) 9. RE: lighted floatation Bath (David Haagsma) 10. Anyone looking for an Ultra Low Freezer (Amber Tyler ANTYLER) 11. FW: Re: Collagen stain for paraffin sections (Sherwood, Margaret ) 12. If You Can't Go to NSH in AZ We Have an Alternative in October (Pamela Marcum) 13. Peloris tissue processor (lhartman18@adelphia.net) 14. QUALIFICATIONS FOR SUPERVISOR (Vickroy, Jim) 15. RE: Peloris tissue processor (Douglas D Deltour) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Sep 2006 15:15:25 -0400 From: "HSRL" Subject: [Histonet] HSRL Job Opportunities To: Message-ID: <000701c6d056$7d12b260$0700a8c0@HSRLMAIN> Content-Type: text/plain; charset="iso-8859-1" Dear Netters, Histo-Scientific Research Laboratories (HSRL, Inc.) is looking to add two full time histotechs and one part time histotech to our team. HSRL is a privately-owned histopathology laboratory located in the Shenandoah Valley of Virginia. We are about 1 ? hours SW of Washington DC tucked away in the beautiful Blue Ridge Mountains. The ideal candidate would have skills in basic paraffin histology as well as plastics (GMA and/or MMA) histology. We offer a full benefits package along with competitive wages, paid vacation days, and a FRIENDLY and RELAXED ATMOSPHERE! If you are interested in learning more about the opportunities at HSRL, please fax or e-mail resume to: Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 FAX: 540.477.4448 tomgalati@hsrl.org www.hsrl.org Thank you. ------------------------------ Message: 2 Date: Mon, 4 Sep 2006 18:14:44 -0500 From: "Johnson, Teri" Subject: [Histonet] RE: Co-localization studies... To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" >> I am a little bit ashamed, but I have a very basic question to put you: when we are doing co-localization studies (IF) both primary antibodies should not be raised in the same species. Why? What would be the result? I would like to read a little bit more about this subject, where should I look for? Thank you, F?tima<< Fatima - you can do this easily if you have abundant protein and directly labeled antibodies. However, if you are using unlabeled primary antibodies, you need a secondary antibody to complex with the first which adds either an enzyme, biotin, or a fluorescent label. The secondary antibody is usually targeted against the animal species the first antibody was made in. Therefore, if both your primary antibodies are rabbit, and you use a green fluorescent labeled anti-rabbit and a red fluorescent labeled anti-rabbit, both labels will bind with the rabbit species. Everywhere both antibodies bound with the target protein will label with both red and green. Even though that will show colocalization, it does not give you the option of seeing each color channel separately. What if your antigens do not colocalize in every cell? It will look as though it does by doing your immunostaining this way. If primary antibody #1 is located in cell type A and cell type C, and primary antibody #2 is located in cell type B and cell type C, they should only co-localize in C. But by doing it with your method, you will also show colocalization in cell types A, B, and C. Does this make sense? If you use two primary antibodies raised in different species, say goat for Antibody #1 and rabbit for Antibody #2, you can use an anti-goat green fluorescent labeled secondary antibody, and an anti-rabbit red fluorescent labeled secondary antibody. The anti-goat should localize your Antibody #1, and the anti-rabbit should localize your Antibody #2. Therefore, given the above example, Antibody #1 will show positivity in cell type A and C, Antibody #2 will show positivity in cell type B and C, and they will only co-local ize in cell type C. For more information on multiple immunostaining methods, I urge you to purchase and read (cover to cover) Chris van der Loos' book, ISBN 038791594X. Best wishes, Teri Johnson Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 3 Date: Tue, 5 Sep 2006 06:34:00 -0500 From: "Chris Pomajzl" Subject: [Histonet] Sakura Coverslipper Timing To: "HISTONET" Message-ID: <001a01c6d0df$2f491180$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" Histonetters: We have 2 Sakura coverslippers that are in desperate need of some TLC, maintenance and repair. They are 7+ years old, and have been used and abused for all of those years. They are shared equipment between Histo and Cytology. Therefore, there has been 7 years of PAP juice buildup. Our in-house BioMed tech retired a couple of years ago, and ever since, we have used a local BioMed company that does not have much experience with these machines. They would come out and temporarily fix any problems, but it would only correct the problem for a week or two. As a result I have to do much of the maintenance and repair myself. I am somewhat mechanically inclined, but I have my limits. I finally disassembled one of the machines last week so that I could remove all of the disgusting build-up that was covering all of the parts. I was able to put it back together, and it appears to be in good working order, except for one problem. I cannot get the timing of the solenoid in sync with the lever arm that allows the receiving basket to drop one notch of the basket at a time. The service manual explains how to fix the timing, but I don't know that I understand these directions clearly. It just doesn't make much sense, and I simply am not sure I am doing it correctly. Does anyone have any experience who could offer some tips and advice? It would be much appreciated. We are in the process of replacing one of these machines, but that will take some time, I'm sure. And that still leaves me with one old machine. Chris Pomajzl, BS, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. ------------------------------ Message: 4 Date: Tue, 5 Sep 2006 06:41:42 -0700 (PDT) From: Steven Coakley Subject: [Histonet] lighted floatation Bath To: Histonet@lists.utsouthwestern.edu Message-ID: <20060905134142.5601.qmail@web38209.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I'm looking for a working lighted floatation bath as a primary for our lab. --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. ------------------------------ Message: 5 Date: Tue, 05 Sep 2006 09:52:37 -0400 From: Kathleen Roberts Subject: Re: [Histonet] lighted floatation Bath To: Steven Coakley Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <44FD8125.3020403@rci.rutgers.edu> Content-Type: text/plain; charset=windows-1252; format=flowed I like this one from Fisher Scientific: https://www1.fishersci.com/Coupon?cid=1328&gid=137998 Kathleen Roberts Neurotoxicology Labs Rutgers University Steven Coakley wrote: >I'm looking for a working lighted floatation bath as a primary for our lab. > >--------------------------------- >How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 05 Sep 2006 06:58:10 -0700 From: Andrea Grantham Subject: [Histonet] Phoenix To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20060905065316.00c7f678@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed This week many of you will be traveling to Phoenix for the 32nd NSH Symposium/Convention and the Arizona Committee is anxiously awaiting for your arrival. We hope that you have a wonderful visit to Phoenix and Arizona. We have ordered cooler temperatures for you and maybe a bit of late summer monsoons. Please stop by the Phoenix PR table in the Convention Center to say hello. Safe travels! ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 7 Date: Tue, 5 Sep 2006 10:03:51 -0400 From: "Markus F. Meyenhofer" Subject: Re: [Histonet] lighted floatation Bath To: "Steven Coakley" , Message-ID: <082d01c6d0f4$1e1b2190$26893cd1@DJ4VDH31> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Several available, also other histo and EM equipment. Please contact me off list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 ----- Original Message ----- From: "Steven Coakley" To: Sent: Tuesday, September 05, 2006 9:41 AM Subject: [Histonet] lighted floatation Bath > I'm looking for a working lighted floatation bath as a primary for our > lab. > > --------------------------------- > How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call > rates. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Tue, 5 Sep 2006 09:56:56 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCID\)" Subject: RE: [Histonet] lighted floatation Bath To: "Steven Coakley" , Message-ID: Content-Type: text/plain; charset="us-ascii" I LOVE the one Surgipath sells. It doesn't have the glass insert so it heats very rapidly and has a black background. You want the square one, not the round one. It's product number 04535S. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, September 05, 2006 9:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] lighted floatation Bath I'm looking for a working lighted floatation bath as a primary for our lab. --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 5 Sep 2006 10:06:47 -0400 From: "David Haagsma" Subject: RE: [Histonet] lighted floatation Bath To: "Kathleen Roberts" , "Steven Coakley" Cc: histonet@lists.utsouthwestern.edu Message-ID: <19E3602A16438E48B51A4250CA04B5F680AE9B@exchange.marketlab.com> Content-Type: text/plain; charset="us-ascii" Barnstead has a much newer model with LED temperature readout, separate light and power switches and a temp probe for less money. http://www.barnsteadthermolyne.com/search_modelnbr.cfm Product Group:Histology Product Category:TISSUE FLOAT BATH Model Number: 26106Q You have to plug the model number into the search field to see the product. Dave Haagsma -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Roberts Sent: Tuesday, September 05, 2006 9:53 AM To: Steven Coakley Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] lighted floatation Bath I like this one from Fisher Scientific: https://www1.fishersci.com/Coupon?cid=1328&gid=137998 Kathleen Roberts Neurotoxicology Labs Rutgers University Steven Coakley wrote: >I'm looking for a working lighted floatation bath as a primary for our lab. > >--------------------------------- >How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 5 Sep 2006 11:41:11 -0400 From: Amber Tyler ANTYLER Subject: [Histonet] Anyone looking for an Ultra Low Freezer To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Just posting to tell everyone that New Brunswick Scientific has a discount offer on Ultralow freezers. I just received a quote today for half price. Thank you, Amber N. Tyler, Ph.D. Research Scientist Dept of Psychology (PO Box 26170) 296 Eberhart Building, Walker Avenue University of North Carolina - Greensboro Greensboro, NC 27402 ------------------------------ Message: 11 Date: Tue, 5 Sep 2006 11:54:14 -0400 From: "Sherwood, Margaret " Subject: [Histonet] FW: Re: Collagen stain for paraffin sections To: Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30BC3@PHSXMB1.partners.org> Content-Type: text/plain; charset="iso-8859-1" To all: Does anyone know if the collagen stain, Masson Trichrome, would deliniate denatured or necrotic collagen from normal collagen in paraffin sections? One of the researchers is looking for such a stain. If not, is there another collagen stain that would? (We routinely use MTC). Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org ------------------------------ Message: 12 Date: Tue, 05 Sep 2006 12:15:25 -0400 From: Pamela Marcum Subject: [Histonet] If You Can't Go to NSH in AZ We Have an Alternative in October To: Histonet@lists.utsouthwestern.edu Message-ID: <6.1.1.1.2.20060905115941.01a24ab8@mail.vet.upenn.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed The Pennsylvania Histotechnology Society is holding an October seminar program in the Pittsburgh area. If you could not go to the NSH Symposium in Phoenix and you will still need to complete CEUs this year join us for the meeting. We will have 3 days of special seminars covering management issues, beginning and advanced IHC classes, forensics autopsy and entomology to name a few. Go to our web site at www.pahisto.org for a full program and registration forms. The meeting will be in Cranberry, PA just outside Pittsburgh so we encourage members of NSH, Ohio, New York, West Virginia and all of Region II to join us in an educational/fun experience. We will have vendor exhibits and a special workshop on automated special stainers they will participate in. We have workshops and talks planned for all phases of Histology and many related fields. We will have a talk on FNA procedures for cytology/histology. We will have safety and autopsy workshops for PAs and others who will be required to have CEUs in these areas. Plese contact me if you have any questions. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ------------------------------ Message: 13 Date: Tue, 5 Sep 2006 9:25:40 -0700 From: Subject: [Histonet] Peloris tissue processor To: histonet@lists.utsouthwestern.edu Message-ID: <6769591.1157473540991.JavaMail.root@web18> Content-Type: text/plain; charset=utf-8 Would you please share your experiences with the Peloris tissue processor by visionbiosystems? Any comments would be appreciated. Thanks, Linda Hartman Histology Section Head Mount Nittany Medical Center State College, PA 16803 814-234-6117 X6650 email lhartman@mountnittany.org ------------------------------ Message: 14 Date: Tue, 5 Sep 2006 11:48:40 -0500 From: "Vickroy, Jim" Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR To: Message-ID: Content-Type: text/plain; charset="us-ascii" Are there folks supervising histology laboratories that only have an HT certification and not a degree? And............. What do the governing organizations say about this? I know I can get the information but wondered if anyone had a quick answer. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 15 Date: Tue, 5 Sep 2006 12:56:11 -0400 From: "Douglas D Deltour" Subject: RE: [Histonet] Peloris tissue processor To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Run away! Ask them for a list of users. I have one sitting in my storage area waiting on them to pick it up. It has never worked right and the tech support is non existent. The techs do not know how to fix it. It is the second one that they have sent us. If anyone from Vision is reading this please have it removed from our storage area. It has been here for four months waiting on you to pick it up. I hope the acquisition of this company doesn't tarnish the Ventana reputation. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lhartman18@adelphia.net Sent: Tuesday, September 05, 2006 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Would you please share your experiences with the Peloris tissue processor by visionbiosystems? Any comments would be appreciated. Thanks, Linda Hartman Histology Section Head Mount Nittany Medical Center State College, PA 16803 814-234-6117 X6650 email lhartman@mountnittany.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 34, Issue 5 *************************************** Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From MVaughan4 <@t> ucok.edu Tue Sep 5 12:47:44 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Tue Sep 5 12:48:13 2006 Subject: [Histonet] re: Co-localization studies... In-Reply-To: Message-ID: Fatima, If you are using direct immunofluorescence, two primaries from the same species should not matter as long as they are fluorescently labeled differently. However, if you are using indirect immunofluroescence, the secondary antibody would recognize both primaries and you could not tell which stain was which. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm Hello Histonetters, I am a little bit ashamed, but I have a very basic question to put you: when we are doing co-localization studies (IF) both primary antibodies should not be raised in the same species. Why? What would be the result? I would like to read a little bit more about this subject, where should I look for? Thank you, F?tima -- F?tima Ferreirinha IBMC R. do Campo Alegre, 823 4150-180, Porto Portugal From RJLevier <@t> LancasterGeneral.org Tue Sep 5 13:00:40 2006 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Tue Sep 5 13:00:53 2006 Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2D6E054@MAIL-LR.lha.org> Just to let you know, you can have an associates degree in histology and have the HT certification. It is not just those who OJT'd that have an HT. Most schools currently are offering an associates degree for you to take the HT exam. Most job postings that I see are asking for either an HT or an HTL for the supervisory position. Becky -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 05, 2006 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR Are there folks supervising histology laboratories that only have an HT certification and not a degree? And............. What do the governing organizations say about this? I know I can get the information but wondered if anyone had a quick answer. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gu.lang <@t> gmx.at Tue Sep 5 13:05:21 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Sep 5 13:05:27 2006 Subject: [Histonet] freeze or not to freeze Message-ID: <000501c6d115$da8a4150$eeeea8c0@SERVER01> Hi, I hope one of the IHC-specialists can explain to me, why some of the antibody-concentrats are not allowed to be stored at -20?C in aliquots. What is the difference to the others? Must I take this information on the datasheet seriously? Thank you Gudrun Lang From kimtournear <@t> yahoo.com Tue Sep 5 13:06:22 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Sep 5 13:06:26 2006 Subject: [Histonet] Florida Histology License Message-ID: <20060905180622.4762.qmail@web37703.mail.mud.yahoo.com> Can anyone out there tell me how to find out about getting a Florida Histology license? Is there a web site, phone#, etc.....I don't live there, but a friend of mine is looking to relocate out there in the near future and would like to get it before excepting any offers....Thanks Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From Kari.Zajic <@t> HCAhealthcare.com Tue Sep 5 13:18:41 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Sep 5 13:18:52 2006 Subject: [Histonet] Florida Histology License In-Reply-To: <20060905180622.4762.qmail@web37703.mail.mud.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC633@ORLEV03.hca.corpad.net> Hi Kim! You can contact the Florida Clinical Laboratory Personnel Board/FDOH by visiting www.doh-mqaservices.com or calling 850-488-0595 menu option #3. They are very nice and helpful! There is a company for CEU's that you may require (I am not sure) but they know all of the requirements and laws and are very professional and nice! They might be able to help as well. Anderson Continuing Education 1-800-532-2332 Good luck!! We need more techs in Florida!!! :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kim Tournear Sent: Tuesday, September 05, 2006 2:06 PM To: Histonet Subject: [Histonet] Florida Histology License Can anyone out there tell me how to find out about getting a Florida Histology license? Is there a web site, phone#, etc.....I don't live there, but a friend of mine is looking to relocate out there in the near future and would like to get it before excepting any offers....Thanks Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Sep 5 13:17:27 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Sep 5 13:19:08 2006 Subject: [Histonet] Florida Histology License References: <20060905180622.4762.qmail@web37703.mail.mud.yahoo.com> Message-ID: Here it is: Florida Health and Rehabilitative Services - www.doh-mqaservices.com or telephone:850-245-4444 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Tournear Sent: Tue 9/5/2006 2:06 PM To: Histonet Subject: [Histonet] Florida Histology License Can anyone out there tell me how to find out about getting a Florida Histology license? Is there a web site, phone#, etc.....I don't live there, but a friend of mine is looking to relocate out there in the near future and would like to get it before excepting any offers....Thanks Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> gmhsc.com Tue Sep 5 14:40:57 2006 From: ASelf <@t> gmhsc.com (Amy Self) Date: Tue Sep 5 14:38:32 2006 Subject: [Histonet] Histology PI Manual Message-ID: <39836CD6DB61654E8F95A35898C9218602E4EF12@exchange.gmhpost.com> Does anyone out in histoland have the manual "Histopathology Laboratory Performance Improvement and Leadership Manual". and if so how do you like it. I rec'd a fax today about this manual and was just curious if anyone out there has it on hand. Thanks in advance. Amy Amy Self Georgetown Hospital 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From AGrobe2555 <@t> aol.com Tue Sep 5 14:38:48 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Sep 5 14:38:57 2006 Subject: [Histonet] Co-localization studies Message-ID: In your case, if you do 2 rounds of staining and detect each primary individually with a different-colored secondary, you should have no problem. It is indeed more convenient if the primaries are from different species, as you can detect both simultaneously with different-colored secondaries. This saves quite a bit of time. You could also used direct-conjugated primary antibodies, but the sensitivity may be reduced. Albert Albert C. Grobe, PhD International Heart Institute, Tissue Engineering Lab Saint Patrick Hospital From beckyjo <@t> email.unc.edu Tue Sep 5 14:40:54 2006 From: beckyjo <@t> email.unc.edu (Rebecca Jo) Date: Tue Sep 5 14:40:06 2006 Subject: [Histonet] Peptide Competition Message-ID: <20060905154054.dylg1mkc78k8sk40@webmail4.isis.unc.edu> Hello Histoneters- I am doing peptide competitions (in tissue) with our antibody, but I've been getting some puzzling results. I'm incubating primary antibody with specific or non-specific peptide (10X concentration of primary) overnight at 4C. Then I spin them down and proceed with my usual staining protocol. I also use a positive control where I use just primary antibody with no peptide which is also incubated overnight and spun down. My problem is that I'm getting a pretty weak signal in both primary alone and primary + non-specific peptide. Is it possible that my signal weakens because of the extra day of incubation (incubation with primary + peptide overnight and then incubation of primary/peptide with tissue overnight)? If so, why would this be? I'm asking this because when I stain with our antibody I get a very strong signal, but add that extra day of incubation for the peptide competition and my signal goes way down. Any suggestions as to what might be going on here? Thanks, -- Rebecca E. Jo UNC-School of Medicine Department of Cell and Developmental Biology (919)843-9648 CB# 7090 From pruegg <@t> ihctech.net Tue Sep 5 14:48:01 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Sep 5 14:48:08 2006 Subject: [Histonet] more rigid paraffin Message-ID: <000501c6d124$32c384e0$6601a8c0@Patsy> Does anyone know of a paraffin which will stretch less/distort less than most? We are trying to digitally reconstruct tissue 3 dimensionally at the histology level by scanning tissue sections completely thru the sample and line them up one after the other but the paraffin sections stretch and distort so that one section does not match it's deeper counter part. We thought if we had a stiffer medium this distortion from section to deeper section would be reduced? We are thinking about using GMa instead paraffin to embed expecting less distortion but we also want to do IHC and that is problematic with GMA. Any suggestions for this project would be appreciated. Thanks, Patsy Ruegg IHCtech 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net From PMonfils <@t> Lifespan.org Tue Sep 5 15:15:51 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Sep 5 15:16:10 2006 Subject: [Histonet] more rigid paraffin Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717799@lsexch.lsmaster.lifespan.org> The problem is, tissues shrink during processing, specifically during dehydration. However, tissue has "memory", and on the water bath the tissue section relaxes and stretches back to approximately its original dimensions. The embedding medium has to stretch to accomodate the stretching of the tissue. If the water bath is too warm, some tissues may overstretch, causing separations within the tissue. If it is too cool, the tissue may not resume its original dimensions, and may have wrinkles in it. An embedding medium that doesn't stretch well would probably have the same effect as a water bath that is too cool, preventing the tissue section from assuming its true size and shape. If your sections are overstretching, perhaps you could try setting your water bath a couple of degrees cooler. Or, you might have to go a couple of degrees warmer. Inconsistent stretching can be caused by either condition. And/or you could try adding a drop of detergent to your water bath, to lessen the effects of surface tension on the tissue sections. Paul M. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patsy > Ruegg > Sent: Tuesday, September 5, 2006 12:48 PM > To: histonet@pathology.swmed.edu > Subject: [Histonet] more rigid paraffin > > Does anyone know of a paraffin which will stretch less/distort less than > most? We are trying to digitally reconstruct tissue 3 dimensionally at > the > histology level by scanning tissue sections completely thru the sample and > line them up one after the other but the paraffin sections stretch and > distort so that one section does not match it's deeper counter part. We > thought if we had a stiffer medium this distortion from section to deeper > section would be reduced? We are thinking about using GMa instead > paraffin > to embed expecting less distortion but we also want to do IHC and that is > problematic with GMA. Any suggestions for this project would be > appreciated. > > Thanks, > > Patsy Ruegg > > IHCtech > > 12635 Montview Blvd. Ste.215 > > Aurora, Colorado 80045 > > Phone: 720-859-4060 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Tue Sep 5 16:06:53 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 5 16:07:00 2006 Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR In-Reply-To: Message-ID: <20060905210653.91367.qmail@web61219.mail.yahoo.com> Jim: Regulations vary state by state. In Florida you have to have a Supervisor's license, and to have that you have to have certain study credentials (with some "grandfathering" of course in some cases). Otherwise assigning the supervision of a histology lab either as a FTE supervisor, "bench" supervisor or lead tech is mostly an internal lab decision sometimes briven by non technical considerations. Ren? J. "Vickroy, Jim" wrote: Are there folks supervising histology laboratories that only have an HT certification and not a degree? And............. What do the governing organizations say about this? I know I can get the information but wondered if anyone had a quick answer. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From laurie.colbert <@t> huntingtonhospital.com Tue Sep 5 16:14:50 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Sep 5 16:14:57 2006 Subject: [Histonet] CAP Question Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653D10@EXCHANGE1.huntingtonhospital.com> Can anyone help explain what kind of policy I would need for the following CAP question: ANP.22615: If the laboratory uses an avidin-biotin complex (ABC) detection system (or a related system such as streptavidin-biotin or neutravidin-bioton), is there a policy that addresses nonspecific false positive staining from endogenous bioton? Laurie Colbert Huntington Hospital From hancockestates <@t> yahoo.com Tue Sep 5 16:31:00 2006 From: hancockestates <@t> yahoo.com (MaryAnn Hancock) Date: Tue Sep 5 16:31:05 2006 Subject: [Histonet] (no subject) Message-ID: <20060905213101.14392.qmail@web51603.mail.yahoo.com> What is the fixation time people use for Breast Core Biopsies...minimum time required. Thanks MA --------------------------------- Get your email and more, right on the new Yahoo.com From tpmorken <@t> labvision.com Tue Sep 5 16:34:26 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Tue Sep 5 16:34:31 2006 Subject: FW: [Histonet] QUALIFICATIONS FOR SUPERVISOR Message-ID: Jim, Short answer: Yes, an HT with no degree can supervise a lab. In the end the pathologist laboratory director is responsible for what comes out of pathology and he/she can delegate duties to whomever they wish (in fact, the pathologist lab director has sole technical responsibility for histology lab results. No histotech anywhere that I am aware of signs out anything). The duties in the supervisor role are administrative by definition so do not require any special skills beyond administration. If the person is involved in testing procedures then they need to show proficiency for that, but that is another matter. I have been in labs in which the supervisor was a managerial-type person with no knowledge of histology. They also had "senior techs" who handled the technical side. It worked out ok since the supervisor did not delve into technical matters beyond financial concerns. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 05, 2006 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR Are there folks supervising histology laboratories that only have an HT certification and not a degree? And............. What do the governing organizations say about this? I know I can get the information but wondered if anyone had a quick answer. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amanda.Garcia <@t> TriadHospitals.com Tue Sep 5 17:28:14 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Tue Sep 5 17:43:36 2006 Subject: [Histonet] Cryostat decontaminant Message-ID: <8B63039C9DF4554C8FDBF31235F0E14801EF26E1@CPRTEVS02.triadhospitals.net> Dear fellow histo-netters, Could someone help me out with this question. Is 70% alcohol a sufficient decontaminant to use for our bi-annual defrost and decontamination of the cryostat? Any help would be greatly appreciated. If it is not sufficient, could someone please forward a disinfectant that they use? Thanks in advance, Amanda > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From tahseen <@t> brain.net.pk Tue Sep 5 20:10:22 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Tue Sep 5 20:10:31 2006 Subject: [Histonet] (no subject) References: <20060905213101.14392.qmail@web51603.mail.yahoo.com> Message-ID: <001701c6d151$3addfa20$6d0980cb@p> Dear MA 10%Buffred Neutral Formalin Solution for 4hours. Muhammad Tahseen ----- Original Message ----- From: "MaryAnn Hancock" To: Sent: Wednesday, September 06, 2006 2:31 AM Subject: [Histonet] (no subject) > What is the fixation time people use for Breast Core Biopsies...minimum > time required. > Thanks > MA > > > --------------------------------- > Get your email and more, right on the new Yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> adelphia.net Tue Sep 5 20:35:14 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Tue Sep 5 20:35:24 2006 Subject: [Histonet] Peloris tissue processor In-Reply-To: <20060905170546.PKVU6179.aamta2.adelphia.net@swlx162.swmed.edu> Message-ID: <000401c6d154$b3df9430$2ea2ac45@D6WRV2B1> We have been using our new Peloris for 5 weeks with great results. So far so good along with good tech support. Steve Westra (Florida rep) and Rick Couture (Mass. tech support) have been great with us. Ron Martin, BS, HTL(ASCP) Histology Supervisor Advanced Dermatology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 05, 2006 12:56 PM To: lhartman18@adelphia.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peloris tissue processor Run away! Ask them for a list of users. I have one sitting in my storage area waiting on them to pick it up. It has never worked right and the tech support is non existent. The techs do not know how to fix it. It is the second one that they have sent us. If anyone from Vision is reading this please have it removed from our storage area. It has been here for four months waiting on you to pick it up. I hope the acquisition of this company doesn't tarnish the Ventana reputation. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lhartman18@adelphia.net Sent: Tuesday, September 05, 2006 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Would you please share your experiences with the Peloris tissue processor by visionbiosystems? Any comments would be appreciated. Thanks, Linda Hartman Histology Section Head Mount Nittany Medical Center State College, PA 16803 814-234-6117 X6650 email lhartman@mountnittany.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Wed Sep 6 00:52:01 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Wed Sep 6 01:07:29 2006 Subject: [Histonet] freeze or not to freeze References: <000501c6d115$da8a4150$eeeea8c0@SERVER01> Message-ID: <003f01c6d178$a6fc9690$27955c82@patho.unibe.ch> Hi Gudrun we generally store antibodies according to the manufacturer's recommendations - today for most antibodies this is between 2 and 8?C. However, we do aliquot antibodies that we use only very rarely. When we aliquot and freeze, we make sure that there is some protein in the antibody solution besides the antibody (e.g. BSA, serum proteins, etc.). Further, we store aliquots at -80?C, not at -20?C. The reason is mainly the quality of the freezers: most "household-type" freezers have an automatic defrost cycle which results in a very dry environment in the freezer. This leads to "freeze drying" of the aliquots over time. With -80?C freezers the problem is somewhat reduced (they have no defrost cycle, however, frequent opening of the -80?C freezer can result in a similar effect). Nevertheless we try to freeze aliquots of a volume no smaller than approx. 50 ul (predilute antibody if necessary) and we never use Eppendorf type tubes, as their lid is not as tightly closed as when you use screw cap tubes with a sealing (e.g. Sarstedt and others). Freezing is generally not recommended for labeled antibodies (biotin, fluorescent labels, etc.), as the label may be detached. If we have labeled antibodies that we wish to store at lower temperatures, because we need only very small amounts per year, then we add glycerol up to 50% (v/v) and store at -20?C. The glycerol keeps the solution in liquid phase and avoids the mechanical stress on the label that you get when you freeze (--> liquid to solid phase). This has worked fine with us and we have antibodies stored that way for years - and when they come out of the freezer, they still work. Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Gudrun Lang" To: "Histonetliste (Histonetliste)" Sent: Tuesday, September 05, 2006 8:05 PM Subject: [Histonet] freeze or not to freeze > Hi, > I hope one of the IHC-specialists can explain to me, why some of the > antibody-concentrats are not allowed to be stored at -20?C in aliquots. > What > is the difference to the others? Must I take this information on the > datasheet seriously? > > Thank you > Gudrun Lang > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gillian.2.brown <@t> gsk.com Wed Sep 6 03:00:44 2006 From: gillian.2.brown <@t> gsk.com (gillian.2.brown@gsk.com) Date: Wed Sep 6 02:59:36 2006 Subject: [Histonet] more rigid paraffin In-Reply-To: <000501c6d124$32c384e0$6601a8c0@Patsy> Message-ID: Patsy, You need to use a GMA procedure where you start off fixing in ice-cold acetone and the whole infiltration procedure is carried out at 4 degrees, rather than a 'standard GMA method. Brilliant morphology and retained antigenicity and as desired little distortion between sections. However size can be a limitation, 2mm is recommended but I've done whole mouse heads. Look out papers by Susan Wilson or I could send you our protocol (which is hers). Cheers Gill Brown GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 05-Sep-2006 20:48 To histonet@pathology.swmed.edu cc Subject [Histonet] more rigid paraffin Does anyone know of a paraffin which will stretch less/distort less than most? We are trying to digitally reconstruct tissue 3 dimensionally at the histology level by scanning tissue sections completely thru the sample and line them up one after the other but the paraffin sections stretch and distort so that one section does not match it's deeper counter part. We thought if we had a stiffer medium this distortion from section to deeper section would be reduced? We are thinking about using GMa instead paraffin to embed expecting less distortion but we also want to do IHC and that is problematic with GMA. Any suggestions for this project would be appreciated. Thanks, Patsy Ruegg IHCtech 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Sep 6 05:30:47 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Sep 6 05:30:55 2006 Subject: [Histonet] FW: Re: Collagen stain for paraffin sections In-Reply-To: <1AF23D0AD12E7444A5DB083CA978B73407A30BC3@PHSXMB1.partners.org> Message-ID: Hi Peggy, Quite a few of the researchers here like the PicroSirius Red stain (PSR). I am not sure if that is one that is a stain that would help you. I can send you the protocol. Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, September 05, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Re: Collagen stain for paraffin sections To all: Does anyone know if the collagen stain, Masson Trichrome, would deliniate denatured or necrotic collagen from normal collagen in paraffin sections? One of the researchers is looking for such a stain. If not, is there another collagen stain that would? (We routinely use MTC). Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JosefaNava <@t> texashealth.org Wed Sep 6 06:31:50 2006 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Wed Sep 6 06:31:57 2006 Subject: [Histonet] PSMA (PROSTATE SPECIFIC MEMBRANE ANTIGEN) Message-ID: <2C515C1049EAF5459EFD8C9B929078A401DA13B3@phdex03.txhealth.org> Hello Everyone, Can someone tell me where I can order the PSMA antibody that will work well with the Ventana machines. I appreciate your help. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From rjbuesa <@t> yahoo.com Wed Sep 6 07:36:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 6 07:36:05 2006 Subject: [Histonet] Cryostat decontaminant In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E14801EF26E1@CPRTEVS02.triadhospitals.net> Message-ID: <20060906123601.51848.qmail@web61213.mail.yahoo.com> Amanda: We used bleach at the same concentration as we used for countertops. Ren? J. "Garcia, Amanda" wrote: Dear fellow histo-netters, Could someone help me out with this question. Is 70% alcohol a sufficient decontaminant to use for our bi-annual defrost and decontamination of the cryostat? Any help would be greatly appreciated. If it is not sufficient, could someone please forward a disinfectant that they use? Thanks in advance, Amanda > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From TillRenee <@t> uams.edu Wed Sep 6 07:49:09 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Wed Sep 6 07:49:39 2006 Subject: [Histonet] tissues in cleaning cycle Message-ID: <11F927674DEBDC43B960809A7403C5D201DA6266@MAILPED.ad.uams.edu> Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Wed Sep 6 08:01:26 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 6 08:01:34 2006 Subject: [Histonet] tissues in cleaning cycle In-Reply-To: <11F927674DEBDC43B960809A7403C5D201DA6266@MAILPED.ad.uams.edu> Message-ID: <20060906130126.56158.qmail@web61212.mail.yahoo.com> Ren?e: A tissue left in the cleaning cycle is one that is already dehydrated and in the "antemedium" (xylene if that is what you use). That is "normal procedure" if you want to "reinfiltrate" the tissue, so your next step would be to put it BRIEFLY in clean xylene and transfer it to be infiltrated with paraffin. Very likely tat your tissue can be put in the last xylene station and through the paraffin stations. Ren? J. "Till, Renee" wrote: Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From jqb7 <@t> cdc.gov Wed Sep 6 08:00:24 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Wed Sep 6 08:03:24 2006 Subject: [Histonet] tissues in cleaning cycle Message-ID: That happened to me once; a single cassette was accidentally left in. I simply reinfiltrated with paraffin in the last of the processing cycle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Wednesday, September 06, 2006 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissues in cleaning cycle Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vanann702 <@t> skmc.gov.ae Wed Sep 6 08:28:45 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Wed Sep 6 08:27:31 2006 Subject: [Histonet] tissues in cleaning cycle Message-ID: I totally disagree- my vip5's cleaning cycle does xylene then absolute and then has a water wash cycle. If I left a block in my wash cycle it would end up in water so I would certainly reprocess it from at least the 95% station!! If I put it straight into the xylene I would put water into my xylene!!! Just my 5cents worth here in the desert Annie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 06 September 2006 17:01 To: Till, Renee; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissues in cleaning cycle Ren?e: A tissue left in the cleaning cycle is one that is already dehydrated and in the "antemedium" (xylene if that is what you use). That is "normal procedure" if you want to "reinfiltrate" the tissue, so your next step would be to put it BRIEFLY in clean xylene and transfer it to be infiltrated with paraffin. Very likely tat your tissue can be put in the last xylene station and through the paraffin stations. Ren? J. "Till, Renee" wrote: Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Sep 6 08:31:52 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Wed Sep 6 08:38:02 2006 Subject: [Histonet] tissues in cleaning cycle Message-ID: You are right: it depends on your cleaning cycle. We do not use formalin on our VIP so we only have xylene and alcohol on the cleaning cycles. Of course if the last step is something other than xylene it must be stepped back before infiltration. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Van Binsbergen Sent: Wednesday, September 06, 2006 9:29 AM To: Rene J Buesa; Till, Renee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissues in cleaning cycle I totally disagree- my vip5's cleaning cycle does xylene then absolute and then has a water wash cycle. If I left a block in my wash cycle it would end up in water so I would certainly reprocess it from at least the 95% station!! If I put it straight into the xylene I would put water into my xylene!!! Just my 5cents worth here in the desert Annie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 06 September 2006 17:01 To: Till, Renee; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissues in cleaning cycle Ren?e: A tissue left in the cleaning cycle is one that is already dehydrated and in the "antemedium" (xylene if that is what you use). That is "normal procedure" if you want to "reinfiltrate" the tissue, so your next step would be to put it BRIEFLY in clean xylene and transfer it to be infiltrated with paraffin. Very likely tat your tissue can be put in the last xylene station and through the paraffin stations. Ren? J. "Till, Renee" wrote: Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Sep 6 08:56:00 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Sep 6 08:56:03 2006 Subject: [Histonet] CAP Question In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628005653D10@EXCHANGE1.huntingtonhospital.com> Message-ID: Laurie, We have a statement in our Laboratory Procedure Manual under "Antibody Optimization" that reads: "The selection of avidin biotin (AB) blocking will be dependent on the specimen source. Examples of tissues benefiting from AB block include kidney and liver. It may be necessary to have more than one protocol programmed to offer the choice of blocking or not." Hope this helps, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, September 05, 2006 4:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone help explain what kind of policy I would need for the following CAP question: ANP.22615: If the laboratory uses an avidin-biotin complex (ABC) detection system (or a related system such as streptavidin-biotin or neutravidin-bioton), is there a policy that addresses nonspecific false positive staining from endogenous bioton? Laurie Colbert Huntington Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Wed Sep 6 10:40:20 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Wed Sep 6 10:40:30 2006 Subject: [Histonet] extra$$$ Message-ID: Netters, I just found out we have extra money in our budget. Limitations are purchases need to be made by this Fri9/8 and maximum per item is $10K. Things I think would be helpful to me are an automated H&E stainer and slide labeler. I am probably over the amount with these but if anyone has any great ideas I would be grateful. Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives From pkarlisch <@t> psu.edu Wed Sep 6 11:14:15 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Wed Sep 6 11:14:57 2006 Subject: [Histonet] tissues in cleaning cycle In-Reply-To: References: Message-ID: <44FEBB970200008C0002206F@GWIA02.HERSHEYMED.NET> I agree. The cleaning cycle brings the tissue back to alcohol. The tissue would need to be reprocessed. We use the cleaning cycle to rehydrate poorly fixed specimens from paraffin back to alcohol. This is a process I learned here that works well. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Anne Van Binsbergen" 9/6/2006 9:28 AM >>> I totally disagree- my vip5's cleaning cycle does xylene then absolute and then has a water wash cycle. If I left a block in my wash cycle it would end up in water so I would certainly reprocess it from at least the 95% station!! If I put it straight into the xylene I would put water into my xylene!!! Just my 5cents worth here in the desert Annie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 06 September 2006 17:01 To: Till, Renee; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissues in cleaning cycle Ren?e: A tissue left in the cleaning cycle is one that is already dehydrated and in the "antemedium" (xylene if that is what you use). That is "normal procedure" if you want to "reinfiltrate" the tissue, so your next step would be to put it BRIEFLY in clean xylene and transfer it to be infiltrated with paraffin. Very likely tat your tissue can be put in the last xylene station and through the paraffin stations. Ren? J. "Till, Renee" wrote: Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mari.ann.mailhiot <@t> leica-microsystems.com Wed Sep 6 11:23:17 2006 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Wed Sep 6 11:23:38 2006 Subject: [Histonet] tissues in cleaning cycle In-Reply-To: <11F927674DEBDC43B960809A7403C5D201DA6266@MAILPED.ad.uams.edu> Message-ID: Renee As long as the last step on the cleaning cycle is 100% alcohol not water you can go to xylene and paraffin. But usually cleaning cycles consist of xylene, 100% alcohol, and water. Water being the last step. Also some cleaning cycleces use heat around 60 degrees. Do you want your specimens subjected to the heat for the amount of time the cleaning cycle runs? It may be something to think about. I don't know what processor you have but you may be able to creat a program that starts with an alcohol like 95%. You may want to check with your processor rep on whether you can try and do it. Hope this helps. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Till, Renee" To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] tissues in cleaning cycle 09/06/2006 07:49 AM Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sjchtascp <@t> yahoo.com Wed Sep 6 11:41:36 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Sep 6 11:41:40 2006 Subject: [Histonet] work wanted Message-ID: <20060906164136.86993.qmail@web38203.mail.mud.yahoo.com> Appears I will be looking for work. I just found out my position is being eliminated. I live in So. WI and will be open to short term out of state or long term/PT/FT within 50 miles. Steve --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From sheila_adey <@t> hotmail.com Wed Sep 6 12:53:55 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Sep 6 12:54:06 2006 Subject: [Histonet] Cryostat decontaminant In-Reply-To: <20060906123601.51848.qmail@web61213.mail.yahoo.com> Message-ID: Hi, We use "Conflikt" by Decon Laboratories. Their email is deconlabs.com Sheila >From: Rene J Buesa >To: "Garcia, Amanda" >,histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Cryostat decontaminant >Date: Wed, 6 Sep 2006 05:36:01 -0700 (PDT) > >Amanda: > We used bleach at the same concentration as we used for countertops. > René J. > >"Garcia, Amanda" wrote: > Dear fellow histo-netters, >Could someone help me out with this question. Is 70% alcohol a >sufficient decontaminant to use for our bi-annual defrost and >decontamination of the cryostat? Any help would be greatly appreciated. >If it is not sufficient, could someone please forward a disinfectant >that they use? > >Thanks in advance, >Amanda > > > > Amanda (Amy) Garcia > > Histology/Pathology > > College Station Medical Center > > (979) 680-5372 office > > (979) 696-5446 fax > > *Mailto:amanda.garcia@triadhospitals.com > > > > This email and any files transmitted with it may contain PRIVILEGED or > > CONFIDENTIAL information and may be read or used only by the intended > > recipient. If you are not the intended recipient of the e-mail or any > > of its attachments, please be advised that you have received this > > e-mail in error and that any use, dissemination, distribution, > > forwarding, printing, or copying of this e-mail or any attached files > > is strictly prohibited. If you have received this e-mail in error, > > please immediately purge it and all attachments and notify the sender > > by reply e-mail or contact the sender at the number listed. > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Do you Yahoo!? > Everyone is raving about the all-new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Deck to dock: outfit your cottage in stylish comfort. Check out Sympatico / MSN Shopping for great Cottage Living ideas. http://shopping.sympatico.msn.ca/category/shp/?bCatID=11,ptnrid=176,ptnrdata=081801 From sheila_adey <@t> hotmail.com Wed Sep 6 13:03:53 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Sep 6 13:04:04 2006 Subject: [Histonet] Deparaffinization times Message-ID: I would like to thank everyone for taking the time to respond to my question about bringing slides to water. The most popular method is 3 changes of Xylene for 5 min. each. We are deffinitely getting better results. Thanks again Sheila _________________________________________________________________ Deck to dock: outfit your cottage in stylish comfort. Check out Sympatico / MSN Shopping for great Cottage Living ideas. http://shopping.sympatico.msn.ca/category/shp/?bCatID=11,ptnrid=176,ptnrdata=081801 From ploykasek <@t> phenopath.com Wed Sep 6 13:06:57 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Sep 6 13:07:07 2006 Subject: [Histonet] Polarizing lenses Message-ID: HI all. I am desperately looking for a source for polarizing lenses for the microscope. Somehow ours have come up missing. What I would like is the simple set up of 2 small, round lenses that can be used with any microscope. Thanks so much. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From PMonfils <@t> Lifespan.org Wed Sep 6 13:14:39 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Sep 6 13:14:46 2006 Subject: [Histonet] Polarizing lenses Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171779C@lsexch.lsmaster.lifespan.org> You can get round polarizing filters in various sizes at any photographic supply store. You can also get them from microscope companies but you'll pay a lot more. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti > Loykasek > Sent: Wednesday, September 6, 2006 11:06 AM > To: histonet > Subject: [Histonet] Polarizing lenses > > HI all. I am desperately looking for a source for polarizing lenses for > the > microscope. Somehow ours have come up missing. What I would like is the > simple set up of 2 small, round lenses that can be used with any > microscope. > > Thanks so much. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From lrichey <@t> u.washington.edu Wed Sep 6 13:34:51 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Wed Sep 6 13:35:04 2006 Subject: [Histonet] tissues in cleaning cycle In-Reply-To: <11F927674DEBDC43B960809A7403C5D201DA6266@MAILPED.ad.uams.edu> References: <11F927674DEBDC43B960809A7403C5D201DA6266@MAILPED.ad.uams.edu> Message-ID: <44FF14CB.7030200@u.washington.edu> They can be reprocessed. We have in the past used the cleaning cycle to reprocess large numbers of under- processed blocks. Till, Renee wrote: >Hello. Any suggestions on what to do with tissues that were accidentally >left in the processor during the cleaning cycle? I would think they need >to be re-infiltrated with paraffin at the very least. I don't know if >our processor will let us do anything but a full processing run. > > > >Renee' Till, HT > >Research Assistant > >Arkansas Children's Nutrition Center > >1212 Marshall St./N2021 > >Little Rock, AR 72002 > >Office (501)364-2785 > >Fax (501)364-3161 > > > > >Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kallred <@t> bellsouth.net Wed Sep 6 15:33:57 2006 From: kallred <@t> bellsouth.net (Kim Allred) Date: Wed Sep 6 15:29:50 2006 Subject: [Histonet] unsubscribe Message-ID: <20060906202946.SPC14159.ibm65aec.bellsouth.net@D6GV9G51> Please take me off the list. I will be out 9/7-10/7. Thanks. From Brandiwork <@t> mchsi.com Wed Sep 6 20:13:01 2006 From: Brandiwork <@t> mchsi.com (Brandi Farris) Date: Wed Sep 6 20:13:41 2006 Subject: [Histonet] Billing question Message-ID: <004f01c6d21a$c47cbe50$14e6d70c@ernie> Hello, Our hospital recently had an insurance/billing consultant give us some advice. We currently perform and bill for a giemsa stain (special stain group I) on all stomach and esophagus biopsy specimens. The consultant told us that we could no longer bill for those. What stain is everyone else doing and how are you billing it? Thanks Brandi Farris From Pathrm35 <@t> adelphia.net Thu Sep 7 06:55:53 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Thu Sep 7 06:56:00 2006 Subject: [Histonet] new employer Message-ID: <000001c6d274$9215cf60$2ea2ac45@D6WRV2B1> Fellow techs, I would appreciate some input on my current situation. I started a new salaried position a few months ago. I figured I would be working about 50 hours a week. I am working 60-70 hours (5-7 days) a week. All my lab personnel are working as much OT as they can. I told my MD that I could not work at this pace for another month while we awaited approval (just approval, not hire) for a new tech. I was told that if I couldn't work all the hours to give my 2 weeks notice. I did give my notice and within 10 minutes I had a new tech hired. My question is this: after seeing his true colors, what would all of you do? Would you stay with him or leave after your probation is up? I know there are plenty of histology positions out there, but trying to find a good company that has a MD that will treat you half way decent is becoming nearly impossible ( here in Florida at least). Sorry for the complaining but I would appreciate some input. Thanks, Ron Martin From Rcartun <@t> harthosp.org Thu Sep 7 06:59:01 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Sep 7 06:59:39 2006 Subject: [Histonet] Billing question In-Reply-To: <004f01c6d21a$c47cbe50$14e6d70c@ernie> References: <004f01c6d21a$c47cbe50$14e6d70c@ernie> Message-ID: <44FFD1450200007700001CCD@hcnwgwds01.hh.chs> I agree with your consultant. Please refer to an article by Wright and Kelly titled "The use of routine special stains for upper gastrointestinal biopsies" that appeared in the March issue of Am J Surg Pathol, Vol. 30, Number 3, pages 357-361. The authors concluded "that routine special stains for all gastric and/or esophageal biopsies are NOT required, and H&E assessment combined with selective ordering of special stains will identify virtually all cases of H. pylori gastritis and intestinal metaplasia". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Brandi Farris" 09/06/06 9:13 PM >>> Hello, Our hospital recently had an insurance/billing consultant give us some advice. We currently perform and bill for a giemsa stain (special stain group I) on all stomach and esophagus biopsy specimens. The consultant told us that we could no longer bill for those. What stain is everyone else doing and how are you billing it? Thanks Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdmd77 <@t> hotmail.com Thu Sep 7 07:02:13 2006 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Thu Sep 7 07:02:19 2006 Subject: [Histonet] Billing question In-Reply-To: <004f01c6d21a$c47cbe50$14e6d70c@ernie> Message-ID: Brandi - The practice of ordering stains "from the bench" in which a special stain is routinely performed on ALL of certain types of specimens is a practice that is frequently performed for the convenience of the laboratory (habits and routines MAY build efficiency) and to reduce turnaround time. In many of these specimens, there are not histologic features on the H&E that would prompt a pathologist to order the giemsa (or other stain). To date, I have not heard of any ruling that does not allow a pathologist/hospital to bill for NECESSARY special stains. However, there is a great deal of inquiry about whether or not practices are billing for unnecessary stains. This could be what the billing consultant is referring to. In your instance you could: - Continue to perform the Giemsas on all gastric and esophageal biopsies (I'm assuming you mean GEJ where there is gastric mucosa?) but only bill for those stains that are INDICATED and REVIEWED because the H&E findings would prompt a giemsa stain. - Discontinue performing Giemsas on ALL gastric and esophageal biopsies and perform Giemsas (or another Group I 88312 or H. pylori immuno (88342) on ONLY those cases in which the stain is indicated by the H&E findings. To appropriately bill for an 88312 - the test should be BOTH indicated and reviewed by the pathologist. A giemsa or H.pylori immuno on a fundic gland polyp is generally NOT indicated (unless there is a great deal of chronic and active inflammation) and billing for an unnecessary test - even if the pathologist reviewed the Giemsa is a risky practice. Not to mention a questionable use of medical dollars. Julia Dahl MD Mosaic Gastrointestinal Research Consortium >From: "Brandi Farris" >To: >Subject: [Histonet] Billing question >Date: Wed, 6 Sep 2006 20:13:01 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc9-f2.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Wed, 6 >Sep 2006 18:14:20 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1GL8Sc-0002nM-Na; Wed, 06 Sep >2006 20:13:42 -0500 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1GL8SZ-0002nF-C0for >histonet@lists.utsouthwestern.edu; Wed, 06 Sep 2006 20:13:40 -0500 >Received: from sccmmhc91.asp.att.net ([204.127.203.211])by >swlx167.swmed.edu with esmtp (Exim 4.44) id 1GL8SY-0004vE-8Nfor >histonet@lists.utsouthwestern.edu; Wed, 06 Sep 2006 20:13:39 -0500 >Received: from ernie (12-215-230-20.client.mchsi.com[12.215.230.20])by >sccmmhc91.asp.att.net (sccmmhc91) with SMTPid <20060907011336m9100sm9t7e>; >Thu, 7 Sep 2006 01:13:37 +0000 >X-Message-Info: LsUYwwHHNt2vN8rsEafwCPGx7mgw5KCGMUKIqYxHo/4= >X-MSMail-Priority: Normal >X-Mailer: Microsoft Outlook Express 6.00.2900.2869 >X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 >X-Scan-Signature: 30210518ac25ee15bb6d4f97c2fc75d4 >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: ef1ad7719a1154f9450b1c67eafa0a9b >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 07 Sep 2006 01:14:20.0474 (UTC) >FILETIME=[F26B51A0:01C6D21A] > >Hello, > >Our hospital recently had an insurance/billing consultant give us some >advice. We currently perform and bill for a giemsa stain (special stain >group I) on all stomach and esophagus biopsy specimens. The consultant told >us that we could no longer bill for those. What stain is everyone else >doing and how are you billing it? > >Thanks >Brandi Farris >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check the weather nationwide with MSN Search: Try it now! http://search.msn.com/results.aspx?q=weather&FORM=WLMTAG From cpomajzl <@t> cpllabs.com Thu Sep 7 07:34:21 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu Sep 7 07:27:57 2006 Subject: [Histonet] new employer References: <000001c6d274$9215cf60$2ea2ac45@D6WRV2B1> Message-ID: <000901c6d279$f21165a0$26fca8c0@CSP> Ron, I feel your pain. We are/were in much the same situation you are. Earlier this year, we had several techs leave because they were able to find better working conditions elsewhere. As a result, we were down 3-4 techs, everyone was working 10-15 hours of OT per week for several months. All new positions needed to be approved before hiring could even begin. Fortunately, we persevered, but it was a very painful process. Stress levels were extrememly high, and the threat of more people leaving was always present. The demand for histotechs right now far outweighs the supply, so that puts the market in the hands of the tech. Consider yourself in a power position of sorts, so be prepared to use it if necessary. Remember that it is a business, and the business side is just "doing business". Try not to take it personally. They are going to try and get away with whatever they can in order to keep costs down and profit margins up. Only you can make this personal decision, and it depends on your specific situation. If you are truly unhappy and can easily find somethig elsewhere, by all means. The only person that is going to look out for you is you. Hope this helps. ----- Original Message ----- From: "Ron Martin" To: Sent: Thursday, September 07, 2006 6:55 AM Subject: [Histonet] new employer > Fellow techs, > > I would appreciate some input on my current situation. I started a new > salaried position a few months ago. I figured I would be working about 50 > hours a week. I am working 60-70 hours (5-7 days) a week. All my lab > personnel are working as much OT as they can. I told my MD that I could not > work at this pace for another month while we awaited approval (just > approval, not hire) for a new tech. I was told that if I couldn't work all > the hours to give my 2 weeks notice. I did give my notice and within 10 > minutes I had a new tech hired. My question is this: after seeing his true > colors, what would all of you do? Would you stay with him or leave after > your probation is up? I know there are plenty of histology positions out > there, but trying to find a good company that has a MD that will treat you > half way decent is becoming nearly impossible ( here in Florida at least). > Sorry for the complaining but I would appreciate some input. > > Thanks, > > Ron Martin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Zajic <@t> HCAhealthcare.com Thu Sep 7 07:29:09 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Sep 7 07:29:17 2006 Subject: [Histonet] Billing question In-Reply-To: <004f01c6d21a$c47cbe50$14e6d70c@ernie> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC64D@ORLEV03.hca.corpad.net> We also bill for a Giemsa on all stomach specimens...if it's being performed, read and diagnosed out, why wouldn't you be able to charge for the work? Did he give you a reason? That's like saying oh, you can't charge for services done... Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brandi Farris Sent: Wednesday, September 06, 2006 9:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question Hello, Our hospital recently had an insurance/billing consultant give us some advice. We currently perform and bill for a giemsa stain (special stain group I) on all stomach and esophagus biopsy specimens. The consultant told us that we could no longer bill for those. What stain is everyone else doing and how are you billing it? Thanks Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Sep 7 08:13:34 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Sep 7 08:14:33 2006 Subject: [Histonet] Billing question In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC64D@ORLEV03.hca.corpad.net> References: <004f01c6d21a$c47cbe50$14e6d70c@ernie> <095327C7CDBDF64B9E9728A54799091E015CC64D@ORLEV03.hca.corpad.net> Message-ID: <44FFE2BE0200007700001CDA@hcnwgwds01.hh.chs> That's not the point. The real question is, "Why are you doing it if it's not medically necessary?". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Zajic Kari" 09/07/06 8:29 AM >>> We also bill for a Giemsa on all stomach specimens...if it's being performed, read and diagnosed out, why wouldn't you be able to charge for the work? Did he give you a reason? That's like saying oh, you can't charge for services done... Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brandi Farris Sent: Wednesday, September 06, 2006 9:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question Hello, Our hospital recently had an insurance/billing consultant give us some advice. We currently perform and bill for a giemsa stain (special stain group I) on all stomach and esophagus biopsy specimens. The consultant told us that we could no longer bill for those. What stain is everyone else doing and how are you billing it? Thanks Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Zajic <@t> HCAhealthcare.com Thu Sep 7 08:31:42 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Sep 7 08:31:53 2006 Subject: [Histonet] Billing question In-Reply-To: <44FFE2BE0200007700001CDA@hcnwgwds01.hh.chs> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC64F@ORLEV03.hca.corpad.net> 99% of the time our biopsies are ordered with R/O H.Pylori from the GI doc in which we prove or disprove with the Giemsa stain. I don't know, not my call but I'd sure want one and would pay for one if I was having a stomach biopsy! I guess it's facility-wide call...it happens to be a policy here put in place by the Medical Director and he's very "old-school", we even keep old handwritten log books here since the inception of the hospital....lucky me. hahaha Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Thursday, September 07, 2006 9:14 AM To: Zajic Kari; histonet@lists.utsouthwestern.edu; Brandi Farris Subject: RE: [Histonet] Billing question That's not the point. The real question is, "Why are you doing it if it's not medically necessary?". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Zajic Kari" 09/07/06 8:29 AM >>> We also bill for a Giemsa on all stomach specimens...if it's being performed, read and diagnosed out, why wouldn't you be able to charge for the work? Did he give you a reason? That's like saying oh, you can't charge for services done... Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brandi Farris Sent: Wednesday, September 06, 2006 9:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question Hello, Our hospital recently had an insurance/billing consultant give us some advice. We currently perform and bill for a giemsa stain (special stain group I) on all stomach and esophagus biopsy specimens. The consultant told us that we could no longer bill for those. What stain is everyone else doing and how are you billing it? Thanks Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Sep 7 08:33:23 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Sep 7 08:32:50 2006 Subject: [Histonet] new employer In-Reply-To: <000001c6d274$9215cf60$2ea2ac45@D6WRV2B1> References: <000001c6d274$9215cf60$2ea2ac45@D6WRV2B1> Message-ID: <45001FA3.5080005@umdnj.edu> Hi Ron: I would stay. The fact that he immediately hired another tech tells me he knows you are right and are not worth loosing. Also, knowing how he operates gives you a leg up in the next round of battles. Good luck! Geoff Ron Martin wrote: >Fellow techs, > >I would appreciate some input on my current situation. I started a new >salaried position a few months ago. I figured I would be working about 50 >hours a week. I am working 60-70 hours (5-7 days) a week. All my lab >personnel are working as much OT as they can. I told my MD that I could not >work at this pace for another month while we awaited approval (just >approval, not hire) for a new tech. I was told that if I couldn't work all >the hours to give my 2 weeks notice. I did give my notice and within 10 >minutes I had a new tech hired. My question is this: after seeing his true >colors, what would all of you do? Would you stay with him or leave after >your probation is up? I know there are plenty of histology positions out >there, but trying to find a good company that has a MD that will treat you >half way decent is becoming nearly impossible ( here in Florida at least). >Sorry for the complaining but I would appreciate some input. > >Thanks, > >Ron Martin > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From histosci <@t> shentel.net Thu Sep 7 10:27:37 2006 From: histosci <@t> shentel.net (HSRL) Date: Thu Sep 7 10:27:51 2006 Subject: [Histonet] Microemboli staining procedure Message-ID: <002801c6d292$296f3550$0700a8c0@HSRLMAIN> Dear Netters, Has anyone used a non-IHC stain to detect microemboli? The Oil Red O method works well on frozen sections, but I am having a difficult time finding a paraffin method. Perhaps PTAH? Any suggestions are appreciated. Thanks, Tom Tom Galati Laboratory Director HSRL, Inc.- A GLP Histopathology Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org From Jackie.O'Connor <@t> abbott.com Thu Sep 7 10:44:28 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Sep 7 10:45:04 2006 Subject: [Histonet] Paraffin dispenser In-Reply-To: Message-ID: I'm posting this for a friend of mine - He is looking for the old type coffee pot paraffin dispenser, along with stainless steel pitchers. Does anyone know where he can buy them new or used? Thanks. Please direct any responses to histonet as well as to Sightdog@comcast.net Thanks. From Jackie.O'Connor <@t> abbott.com Thu Sep 7 10:46:15 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Sep 7 10:46:45 2006 Subject: [Histonet] Anti mouse CD31 In-Reply-To: <002801c6d292$296f3550$0700a8c0@HSRLMAIN> Message-ID: If anyone has any new ideas on CD31 that works in murine tissue, I'll give you a million dollars. (If you'll take a check . . . . . post dated) Wacky Jackie From MMargiotta <@t> bmhmc.org Thu Sep 7 10:49:05 2006 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Thu Sep 7 10:49:19 2006 Subject: [Histonet] bone marrow procedure Message-ID: <922CE5B88F398948B4076A9A4340E7AF03411AB2@bmh_exchange.bmhmc.org> Hi All, Our pathologist is wondering how most labs are processing bone marrow bxs. Are you doing a rapid or slow decal and for how long? If someone could share their procedure, that would be most helpful. Thanks, Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From anh2006 <@t> med.cornell.edu Thu Sep 7 10:54:05 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Thu Sep 7 10:54:11 2006 Subject: [Histonet] Anti mouse CD31 In-Reply-To: References: Message-ID: Everyone (including me) is so obsessed with CD31, but I have moved on. Can't continue to fight a losing battle ... VE-Cadherin is much better - more endothelial cell specific - and easier to work with. Andrea At 10:46 AM -0500 9/7/06, Jackie M O'Connor wrote: >If anyone has any new ideas on CD31 that works in murine tissue, I'll give >you a million dollars. (If you'll take a check . . . . . post dated) > >Wacky Jackie >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From micro <@t> superlink.net Thu Sep 7 11:11:26 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Thu Sep 7 11:11:43 2006 Subject: [Histonet] Paraffin dispenser References: Message-ID: <010701c6d298$4589ab70$dd893cd1@DJ4VDH31> We have a few available. Also other used EM , Histo and lab equipment. List available. Please contact me off list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Thursday, September 07, 2006 11:44 AM Subject: [Histonet] Paraffin dispenser > I'm posting this for a friend of mine - > > He is looking for the old type coffee pot paraffin dispenser, along with > stainless steel pitchers. Does anyone know where he can buy them new or > used? > Thanks. > Please direct any responses to histonet as well as to > > Sightdog@comcast.net > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sheila_adey <@t> hotmail.com Thu Sep 7 11:36:13 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Sep 7 11:36:30 2006 Subject: [Histonet] bone marrow procedure In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF03411AB2@bmh_exchange.bmhmc.org> Message-ID: Hi Michele We allow the core to fix for three hours in 10% NBF, then a 3 hour decal in Richard Allens Cal Rite. Sheila >From: "Margiotta, Michele" >To: >Subject: [Histonet] bone marrow procedure >Date: Thu, 7 Sep 2006 11:49:05 -0400 > >Hi All, >Our pathologist is wondering how most labs are processing bone marrow bxs. >Are you doing a rapid or slow decal and for how long? If someone could >share their procedure, that would be most helpful. > >Thanks, >Michele > > > >This e-mail and any files transmitted with it are confidential and are >intended >solely for the use of the individual or entity to which they are addressed. >This communication may contain material protected by the attorney-client >privilege. If you are not the intended recipient or the person responsible >for >delivering the e-mail to the intended recipient, be advised that you have >received >this e-mail in error and that any use, dissemination, forwarding, printing, >or copying of this e-mail is strictly prohibited. If you have received this >e-mail in >error, please immediately notify the sender via return e-mail or call >Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 From dmccaig <@t> ckha.on.ca Thu Sep 7 11:41:04 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Sep 7 11:46:43 2006 Subject: [Histonet] size of pancreas beta cells on a mouse Message-ID: Hi I know this is a bit off topic but I had a co-workers daughter ask me to find the size of a beta cell of the pancreas on a mouse. I work in a hospital environment and do not have access to information on mice. It is for a school project. She claims she can not find the size on the internet. I would appreciate it if someone in research could provide me the information. sincere thanks Diana From gu.lang <@t> gmx.at Thu Sep 7 11:55:25 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 7 11:55:25 2006 Subject: AW: [Histonet] bone marrow procedure In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF03411AB2@bmh_exchange.bmhmc.org> Message-ID: <000901c6d29e$6ab32970$eeeea8c0@SERVER01> Hi Michele, I think it is an important point to know, how thick and long the cores are. We process bone marrow trephines with 3-4 mm diameter and about 30 mm length. Therefore we let them fix in 8% NBF until the next day of receiving, next day morning we decal them in a bought mixture with formic acid and formaldehyd for 8 hours. Then they are washed in running water for 15 min and put in the usual processing cycle in the VIP. Together with the Ventana IHC system we have HE and IHC on the second day after receiving. Smaller samples will probably require shorter times. Gudrun Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Margiotta, Michele Gesendet: Donnerstag, 07. September 2006 17:49 An: histonet@pathology.swmed.edu Betreff: [Histonet] bone marrow procedure Hi All, Our pathologist is wondering how most labs are processing bone marrow bxs. Are you doing a rapid or slow decal and for how long? If someone could share their procedure, that would be most helpful. Thanks, Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 7 12:09:07 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 7 12:09:16 2006 Subject: [Histonet] new employer In-Reply-To: <000001c6d274$9215cf60$2ea2ac45@D6WRV2B1> Message-ID: <20060907170907.94717.qmail@web61213.mail.yahoo.com> Ron: There have been 2 outcomes to your situation: 1- you know now how your boss REALLY is, and 2- you found out that he prefers to keep you although this does not mean at all that you have him under your thumb. On the other hand you have to determine how do you feel working for him and if the stress is worth while. This is a very personal decision to take and you have to analyze the situation as objectively as possible and you could even discuss your feelings with him and how you realized that he was able to hire somebody when confronted with your resignation. Perhaps talking this over with him will end in a good working relationship. He will realize that you have been frank and forthcoming with him and this is a quality always appreciated. Just my opinion! Ren? J. Ron Martin wrote: Fellow techs, I would appreciate some input on my current situation. I started a new salaried position a few months ago. I figured I would be working about 50 hours a week. I am working 60-70 hours (5-7 days) a week. All my lab personnel are working as much OT as they can. I told my MD that I could not work at this pace for another month while we awaited approval (just approval, not hire) for a new tech. I was told that if I couldn't work all the hours to give my 2 weeks notice. I did give my notice and within 10 minutes I had a new tech hired. My question is this: after seeing his true colors, what would all of you do? Would you stay with him or leave after your probation is up? I know there are plenty of histology positions out there, but trying to find a good company that has a MD that will treat you half way decent is becoming nearly impossible ( here in Florida at least). Sorry for the complaining but I would appreciate some input. Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From rjbuesa <@t> yahoo.com Thu Sep 7 12:15:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 7 12:15:32 2006 Subject: [Histonet] bone marrow procedure In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF03411AB2@bmh_exchange.bmhmc.org> Message-ID: <20060907171525.65052.qmail@web61225.mail.yahoo.com> Michele: Our BM core biosies were fixed for 3-4 in NBF followed by a very GENTLE decalcification with EDTA pH7 overnight. Any other more "rapid" decalcifier always ended reducing nuclear/staining detail when compared with EDTA. Ren? J. "Margiotta, Michele" wrote: Hi All, Our pathologist is wondering how most labs are processing bone marrow bxs. Are you doing a rapid or slow decal and for how long? If someone could share their procedure, that would be most helpful. Thanks, Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From bernaweston <@t> hotmail.com Thu Sep 7 12:31:35 2006 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Thu Sep 7 12:31:43 2006 Subject: [Histonet] Bone Marrows Message-ID: We have had much better bone marrow cores by extendeding the fixation times to 3 and 4 hours, and increasing the rinse time to 30 minutes after decalcification. Trying to make everything so Rapid proves haste makes waste. I like that you can let cores sit overnight in B Plus. Recently our Pathologist suggested extending the B Plus time for the aspirate as well as the core. Any luck with that out there? Bernadette Weston HT From PBugelsk <@t> CNTUS.JNJ.COM Thu Sep 7 12:36:45 2006 From: PBugelsk <@t> CNTUS.JNJ.COM (Bugelski, Peter [CNTUS]) Date: Thu Sep 7 12:36:54 2006 Subject: [Histonet] Anti mouse CD31 Message-ID: <7BF70FA941B9AE4783EAAF733762F1B50B3A5383@CNTUSMAEXS3.na.jnj.com> I've had good luck with murine anti-CD31 in paraffin sections of human xenografts in tissue fixed with a modification of Beckstead's zinc. Tris 12.1 g Calcium chloride 0.5 g Zinc acetate 10 g formalin 13.5 mL Distilled H2O to 900 mL adjust pH until clear Bring to 1000 mL We published using this this fixative in Tang Y et al. CAncer Res 65:3193, 2005 Peter J. Bugelski, Ph.D., FRCPath Senior Research Fellow and Head of Experimental Pathology Centocor R-4-2 200 Great Valley Parkway Malvern, PA 19355 From jdmd77 <@t> hotmail.com Thu Sep 7 12:41:46 2006 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Thu Sep 7 12:41:51 2006 Subject: [Histonet] Billing question In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC64F@ORLEV03.hca.corpad.net> Message-ID: Kari - Please note that this communication is one from a bias of being a fellowship trained GI/Liver pathologist with roughly 55,000 GI/liver case experience over the last six (6) years. A Giemsa or other special stain is sometimes necessary to identify H. pylori - an infection of the stomach that has specific OTHER histologic characteristics on H&E stain. Sometimes, you can see H. pylori on an H&E. The important thing to recognize is that IF those other features aren't there - then the H. pylori won't be either. In the roughly 15,000 gastric biopsies that I have reviewed, about 7500 of them had special stains done "from the bench," and the remaining 7500 I ordered a special stain if I suspected that there was H. pylori or if a "threshold" was crossed. My personal thresholds: (1) any active inflammation with accompanying plasma cells > than 3/interfoveolar space [even if Ithink it's reactive gastropathy with active inflammation - as is seen in bile reflux, NSAIDs and others]; (2) ten or greater plasma cells per interfoveolar space even without active inflammation - in the antrum; (3) six or greater plasma cells per interfoveolar space even without active inflammation - in the body of the stomach; (4) any intestinal metaplasia - that is clearly not junctional pylorus sampling. Anectodally, I have only been surprised by ONE H. pylori stain... in about 15,000 cases... and it ws a case that met my threshold - but barely. About 5% of the cases with the threshold above DO demonstrate H. pylori. These are what many pathologists would call "unsuspected" H. pylori - however, once you know the various patterns that H. pylori can show - in addition to the standard "chronic active gastritis" then the stains can be performed in a directed fashion... eliminating about 70% of all H. pylori stains done from the bench. In all of the "from the bench stains" that I expected to be negative - they were negative. If the clinician writes "r/o H. pylori" - that doesn't REQUIRE a pathologist to do a Giemsa stain. A comparison: if a patient has an iron deficiency anemia and the primary care doctor writes "Rule out colon cancer" on the consultation request - that doesn't REQUIRE the gastroenterologist to take a biopsy of NORMAL COLON if there is no mass there. Julia Dahl, M.D. Gastrointestinal and Hepatic Pathologist Mosaic Gastrointestinal Research Consortium >From: "Zajic Kari" >To: "Richard Cartun" >,, "Brandi Farris" > >Subject: RE: [Histonet] Billing question >Date: Thu, 7 Sep 2006 09:31:42 -0400 > >99% of the time our biopsies are ordered with R/O H.Pylori from the GI doc >in which we prove or disprove with the Giemsa stain. >I don't know, not my call but I'd sure want one and would pay for one if I >was having a stomach biopsy! >I guess it's facility-wide call...it happens to be a policy here put in >place by the Medical Director and he's very "old-school", we even keep old >handwritten log books here since the inception of the hospital....lucky me. >hahaha > >Kari Marie Zajic HTL,MLT >Histology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >telefax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: Richard Cartun [mailto:Rcartun@harthosp.org] >Sent: Thursday, September 07, 2006 9:14 AM >To: Zajic Kari; histonet@lists.utsouthwestern.edu; Brandi Farris >Subject: RE: [Histonet] Billing question > > >That's not the point. The real question is, "Why are you doing it if >it's not medically necessary?". > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Zajic Kari" 09/07/06 8:29 AM >>> >We also bill for a Giemsa on all stomach specimens...if it's being >performed, read and diagnosed out, why wouldn't you be able to charge >for the work? Did he give you a reason? That's like saying oh, you can't >charge for services done... > >Kari Marie Zajic HTL,MLT >Histology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >telefax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If >you are not the intended recipient of the email or any of its >attachment, please be advised that you have received this email in error >and that any use, dissemination, distribution, forwarding, printing, or >copying of this email or any attached files is strictly prohibited. If >you have received this email in error, please immediately purge it and >all attachments and notify the sender by reply email or contact the >sender at the number listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brandi >Farris >Sent: Wednesday, September 06, 2006 9:13 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Billing question > > >Hello, > >Our hospital recently had an insurance/billing consultant give us some >advice. We currently perform and bill for a giemsa stain (special stain >group I) on all stomach and esophagus biopsy specimens. The consultant >told us that we could no longer bill for those. What stain is everyone >else doing and how are you billing it? > >Thanks >Brandi Farris >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check the weather nationwide with MSN Search: Try it now! http://search.msn.com/results.aspx?q=weather&FORM=WLMTAG From wsimms <@t> mcintoshclinic.com Thu Sep 7 12:40:52 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Thu Sep 7 12:51:36 2006 Subject: [Histonet] new employer References: <20060907170907.94717.qmail@web61213.mail.yahoo.com> Message-ID: <004201c6d2a6$3c8c7580$530114ac@w3256domain.com> I would find another job. The guy's obviously a liar, and if he would lie to you about the encumbrances of hiring help, he will lie to you about anything else. Why work for someone you can't trust? Wesley W. Simms ----- Original Message ----- From: "Rene J Buesa" To: "Ron Martin" ; Sent: Thursday, September 07, 2006 1:09 PM Subject: Re: [Histonet] new employer > Ron: > There have been 2 outcomes to your situation: > 1- you know now how your boss REALLY is, and > 2- you found out that he prefers to keep you although this does not mean > at all that you have him under your thumb. > > On the other hand you have to determine how do you feel working for him > and if the stress is worth while. > This is a very personal decision to take and you have to analyze the > situation as objectively as possible and you could even discuss your > feelings with him and how you realized that he was able to hire somebody > when confronted with your resignation. > Perhaps talking this over with him will end in a good working > relationship. He will realize that you have been frank and forthcoming > with him and this is a quality always appreciated. > Just my opinion! > Ren? J. > > > Ron Martin wrote: > Fellow techs, > > I would appreciate some input on my current situation. I started a new > salaried position a few months ago. I figured I would be working about 50 > hours a week. I am working 60-70 hours (5-7 days) a week. All my lab > personnel are working as much OT as they can. I told my MD that I could > not > work at this pace for another month while we awaited approval (just > approval, not hire) for a new tech. I was told that if I couldn't work all > the hours to give my 2 weeks notice. I did give my notice and within 10 > minutes I had a new tech hired. My question is this: after seeing his true > colors, what would all of you do? Would you stay with him or leave after > your probation is up? I know there are plenty of histology positions out > there, but trying to find a good company that has a MD that will treat you > half way decent is becoming nearly impossible ( here in Florida at least). > Sorry for the complaining but I would appreciate some input. > > Thanks, > > Ron Martin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ > countries) for 2?/min or less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Karen.Heckford <@t> CHW.edu Thu Sep 7 12:51:38 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Sep 7 12:55:20 2006 Subject: [Histonet] Cell Smears Message-ID: Hello Everyone, I have never had to do IHC on cell smears only FFPE. I am not sure how to bring them down to buffer before placing them on the Autostainer. Is there something special I need to do? The smears are in 95% alcohol. Do I just place them straight into the buffer? Do I need to rinse them? Augh!!!! HELP!!! Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From billingconsultants <@t> yahoo.com Thu Sep 7 13:00:44 2006 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Thu Sep 7 13:00:48 2006 Subject: [Histonet] Billing Question Message-ID: <20060907180044.56494.qmail@web54214.mail.yahoo.com> We advise our clients to bill for the giemsa stain if it is contributory to the case. If the stain is performed, reviewed and deemed medically necessary then it is a valid charge. Kindest regards, Louri Roberts Billing Consutlants, LLC www.billingconsultants.net --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From wellborj <@t> mercyhealth.com Thu Sep 7 13:19:02 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Thu Sep 7 13:19:40 2006 Subject: [Histonet] new employer Message-ID: Not to change the subject - but how does one continue working with co-workers who do not always tell the truth? Especially whe they mistrained you? I have been working here for just over a year and have been discovering that the others that work here do not always revial the correct ways. I should be able to read the procedures - but they are the ones who wrote them and state that it has changed and the book is not updated. I have taken issue with my managers and it comes up to my word against theirs - they have been here for years - so what to do? >>> "Wesley Simms, MD" 9/7/2006 12:40 PM >>> I would find another job. The guy's obviously a liar, and if he would lie to you about the encumbrances of hiring help, he will lie to you about anything else. Why work for someone you can't trust? Wesley W. Simms ----- Original Message ----- From: "Rene J Buesa" To: "Ron Martin" ; Sent: Thursday, September 07, 2006 1:09 PM Subject: Re: [Histonet] new employer > Ron: > There have been 2 outcomes to your situation: > 1- you know now how your boss REALLY is, and > 2- you found out that he prefers to keep you although this does not mean > at all that you have him under your thumb. > > On the other hand you have to determine how do you feel working for him > and if the stress is worth while. > This is a very personal decision to take and you have to analyze the > situation as objectively as possible and you could even discuss your > feelings with him and how you realized that he was able to hire somebody > when confronted with your resignation. > Perhaps talking this over with him will end in a good working > relationship. He will realize that you have been frank and forthcoming > with him and this is a quality always appreciated. > Just my opinion! > Ren? J. > > > Ron Martin wrote: > Fellow techs, > > I would appreciate some input on my current situation. I started a new > salaried position a few months ago. I figured I would be working about 50 > hours a week. I am working 60-70 hours (5-7 days) a week. All my lab > personnel are working as much OT as they can. I told my MD that I could > not > work at this pace for another month while we awaited approval (just > approval, not hire) for a new tech. I was told that if I couldn't work all > the hours to give my 2 weeks notice. I did give my notice and within 10 > minutes I had a new tech hired. My question is this: after seeing his true > colors, what would all of you do? Would you stay with him or leave after > your probation is up? I know there are plenty of histology positions out > there, but trying to find a good company that has a MD that will treat you > half way decent is becoming nearly impossible ( here in Florida at least). > Sorry for the complaining but I would appreciate some input. > > Thanks, > > Ron Martin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ > countries) for 2?/min or less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Sep 7 14:05:29 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Sep 7 14:05:35 2006 Subject: [Histonet] Cell Smears Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017177A0@lsexch.lsmaster.lifespan.org> You would most likely have no problem transferring smears from 95% alcohol directly into buffer, but when I do such preps I err on the side of caution and place them in 70% alcohol for a few minutes before going into buffer. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Heckford, Karen - SMMC-SF > Sent: Thursday, September 7, 2006 10:51 AM > To: Histonet (E-mail) > Subject: [Histonet] Cell Smears > > <> > Hello Everyone, I have never had to do IHC on cell smears only FFPE. I > am > not sure how to bring them down to buffer before placing them on the > Autostainer. Is there something special I need to do? The smears are in > 95% > alcohol. Do I just place them straight into the buffer? Do I need to > rinse > them? Augh!!!! HELP!!! > Cheers, > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathololgy Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > > From mobine <@t> MIT.EDU Thu Sep 7 14:16:27 2006 From: mobine <@t> MIT.EDU (Hector Mobine) Date: Thu Sep 7 14:16:42 2006 Subject: [Histonet] zinc-fixed paraffin embedding tissue protocol In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE017177A0@lsexch.lsmaster.lifespan.org> Message-ID: <200609071916.k87JGS88018124@outgoing.mit.edu> Hello Everyone, Does anyone have a good protocol for zinc fixing and paraffin embedding of animal tissue? I would greatly appreciate any help. Thanks, Hector ---------------------------------------------------------------------------- Langer Lab Biological Engineering Massachusetts Institute of Technology t: 617-258-8895 f: 617-253-2514 From LBlack <@t> carilion.com Thu Sep 7 15:26:01 2006 From: LBlack <@t> carilion.com (Lisa Black) Date: Thu Sep 7 15:26:20 2006 Subject: [Histonet] Peloris Message-ID: Linda, We received the Vision Biosystems Peloris in April of this year. It is a wonderful machine. The dual retort and shortened process time allows much flexibility. We did have a minor issue once since then and were pleased with the service quality and quick response. This one machine has replaced three traditional processors saving reagent cost and precious floor space. Our lab processes approximately 45,000 surgicals annually with at least 80% currently flowing through the Peloris. Best regards, Lisa Black Histology Manager Carilion Consolidated Lab Roanoke, VA LBLACK@CARILION.COM (540) 985-4082 From rjbuesa <@t> yahoo.com Thu Sep 7 15:39:59 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 7 15:40:03 2006 Subject: [Histonet] new employer In-Reply-To: Message-ID: <20060907203959.60107.qmail@web61217.mail.yahoo.com> Janci: Any lab that prides on being in compliance has a front page in the SOP where it is stated that ALL the employees have read and understand the procedures. Is that way of doing things not implemended in your lab? If that is the case you have nothing to do with your co-workers, this is a manager's issue and you have the right to review the procedures as is their duty to UPDATE THEM. Your issue is more a clear-cut one dealing with mismanagement and supervisory incompetence. Somewhere along the chain of command you have to be able to find somebody that understands your need and correct what is wrong. Ren? J. Janci Wellborn wrote: Not to change the subject - but how does one continue working with co-workers who do not always tell the truth? Especially whe they mistrained you? I have been working here for just over a year and have been discovering that the others that work here do not always revial the correct ways. I should be able to read the procedures - but they are the ones who wrote them and state that it has changed and the book is not updated. I have taken issue with my managers and it comes up to my word against theirs - they have been here for years - so what to do? >>> "Wesley Simms, MD" 9/7/2006 12:40 PM >>> I would find another job. The guy's obviously a liar, and if he would lie to you about the encumbrances of hiring help, he will lie to you about anything else. Why work for someone you can't trust? Wesley W. Simms ----- Original Message ----- From: "Rene J Buesa" To: "Ron Martin" ; Sent: Thursday, September 07, 2006 1:09 PM Subject: Re: [Histonet] new employer > Ron: > There have been 2 outcomes to your situation: > 1- you know now how your boss REALLY is, and > 2- you found out that he prefers to keep you although this does not mean > at all that you have him under your thumb. > > On the other hand you have to determine how do you feel working for him > and if the stress is worth while. > This is a very personal decision to take and you have to analyze the > situation as objectively as possible and you could even discuss your > feelings with him and how you realized that he was able to hire somebody > when confronted with your resignation. > Perhaps talking this over with him will end in a good working > relationship. He will realize that you have been frank and forthcoming > with him and this is a quality always appreciated. > Just my opinion! > Ren? J. > > > Ron Martin wrote: > Fellow techs, > > I would appreciate some input on my current situation. I started a new > salaried position a few months ago. I figured I would be working about 50 > hours a week. I am working 60-70 hours (5-7 days) a week. All my lab > personnel are working as much OT as they can. I told my MD that I could > not > work at this pace for another month while we awaited approval (just > approval, not hire) for a new tech. I was told that if I couldn't work all > the hours to give my 2 weeks notice. I did give my notice and within 10 > minutes I had a new tech hired. My question is this: after seeing his true > colors, what would all of you do? Would you stay with him or leave after > your probation is up? I know there are plenty of histology positions out > there, but trying to find a good company that has a MD that will treat you > half way decent is becoming nearly impossible ( here in Florida at least). > Sorry for the complaining but I would appreciate some input. > > Thanks, > > Ron Martin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ > countries) for 2?/min or less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From rjbuesa <@t> yahoo.com Thu Sep 7 15:45:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 7 15:46:00 2006 Subject: [Histonet] Cell Smears In-Reply-To: Message-ID: <20060907204556.82567.qmail@web61215.mail.yahoo.com> Karen: Yours is a simple case: if your smears were NOT fixed with formalin, you do NOT need to do HIER (and that is what I think you refer to when writing about the buffer). To do IHC in alcohol fixed smears, you just bring them to water, then to the PBS and start the IHC procedure without the HIER step (solely designed to eliminate the crosslinkage due to the NBF fixation). Hope this will help you! Ren? J. "Heckford, Karen - SMMC-SF" wrote: Hello Everyone, I have never had to do IHC on cell smears only FFPE. I am not sure how to bring them down to buffer before placing them on the Autostainer. Is there something special I need to do? The smears are in 95% alcohol. Do I just place them straight into the buffer? Do I need to rinse them? Augh!!!! HELP!!! Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From vazquezr <@t> ohsu.edu Thu Sep 7 15:36:22 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Sep 7 16:05:31 2006 Subject: [Histonet] new employer Message-ID: Janci, It is unfortunate that your manager doesn't see the discrepancies between the manual and the way things are done. Have you told your manager the differences and what they are telling you? Your manager should back you and take some action (update manual). Unfortunately, some managers I SAID SOME :>) don't care as long as the tissue gets out and inspections are past. I worked for a lab that I had two other co-workers harass, verbally abused (even to the supervisor), emotionally abused me and others and nothing was ever done. It even went as far as the Medical Director and HR. I felt my supervisor really let me down, I was the brunt of their abuse and she still did nothing to back me. I got another job. and happy now! Okay, I will stop, just venting. Have wonderful weekend Robyn OHSU From llewllew <@t> shaw.ca Thu Sep 7 16:58:25 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Sep 7 16:58:54 2006 Subject: [Histonet] new employer References: Message-ID: <002301c6d2c8$bec4d2a0$130e4246@yourlk4rlmsu> The correct procedure is given in the lab manual. If other techs are doing it differently they are not complying with the correct procedure and are violating their terms of employment. You should do the procedures as the manual dictates. That is your defence in case of any problems. Your manager now is aware that there may be unauthorised changes to procedures, so if there is problems others are responsible for them, not you. Bryan Llewellyn ----- Original Message ----- From: "Janci Wellborn" To: ; Sent: Thursday, September 07, 2006 11:19 AM Subject: Re: [Histonet] new employer Not to change the subject - but how does one continue working with co-workers who do not always tell the truth? Especially whe they mistrained you? I have been working here for just over a year and have been discovering that the others that work here do not always revial the correct ways. I should be able to read the procedures - but they are the ones who wrote them and state that it has changed and the book is not updated. I have taken issue with my managers and it comes up to my word against theirs - they have been here for years - so what to do? >>> "Wesley Simms, MD" 9/7/2006 12:40 PM >>> I would find another job. The guy's obviously a liar, and if he would lie to you about the encumbrances of hiring help, he will lie to you about anything else. Why work for someone you can't trust? Wesley W. Simms ----- Original Message ----- From: "Rene J Buesa" To: "Ron Martin" ; Sent: Thursday, September 07, 2006 1:09 PM Subject: Re: [Histonet] new employer > Ron: > There have been 2 outcomes to your situation: > 1- you know now how your boss REALLY is, and > 2- you found out that he prefers to keep you although this does not mean > at all that you have him under your thumb. > > On the other hand you have to determine how do you feel working for him > and if the stress is worth while. > This is a very personal decision to take and you have to analyze the > situation as objectively as possible and you could even discuss your > feelings with him and how you realized that he was able to hire somebody > when confronted with your resignation. > Perhaps talking this over with him will end in a good working > relationship. He will realize that you have been frank and forthcoming > with him and this is a quality always appreciated. > Just my opinion! > Ren? J. > > > Ron Martin wrote: > Fellow techs, > > I would appreciate some input on my current situation. I started a new > salaried position a few months ago. I figured I would be working about 50 > hours a week. I am working 60-70 hours (5-7 days) a week. All my lab > personnel are working as much OT as they can. I told my MD that I could > not > work at this pace for another month while we awaited approval (just > approval, not hire) for a new tech. I was told that if I couldn't work all > the hours to give my 2 weeks notice. I did give my notice and within 10 > minutes I had a new tech hired. My question is this: after seeing his true > colors, what would all of you do? Would you stay with him or leave after > your probation is up? I know there are plenty of histology positions out > there, but trying to find a good company that has a MD that will treat you > half way decent is becoming nearly impossible ( here in Florida at least). > Sorry for the complaining but I would appreciate some input. > > Thanks, > > Ron Martin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ > countries) for 2?/min or less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrickbeall <@t> aim.com Thu Sep 7 17:22:56 2006 From: patrickbeall <@t> aim.com (patrickbeall@aim.com) Date: Thu Sep 7 17:23:07 2006 Subject: [Histonet] Frozen Tissue Prep and Fixation Questions for FISH in Spinal Cord and DRG Sections Message-ID: <8C8A0E34D77040C-EC8-3E7B@MBLK-M03.sysops.aol.com> Hi, I'm currently working on studying the expression of chemotropic receptor mRNA in neurons during regenration after an injury. I've got a few questions about the best way to prep the tissue with regards to quality and minimizing trauma that may cause confounding results. 1. What is the best way to extract and fix tissue, and is cryoprotection necessary? Initially I followed GeneDetect's frozen tissue protocol (excise tissue from living animal, snap freeze on foil in a -70 Celsius freezer), but I'm concerned about repeated thawing of the tissue producing artifacts (cryosection, thaw mount on slides, air dry, back to the freezer, back to room temp another day, fix in 4% PFA--I imagine I could fix immediately after drying though, if this is otherwise a decent method). Currently I've been considering just dunking the tissue in a little jar of fix immediately after harvesting, then cryoprotecting in a sucrose based solution prior to cryosectioning. My advisor suggested transcardial fixation, but in that case I'm worried that the anoxia occuring from the time I puncture the diaphragm until the pre-fix wash completes (15-20 minutes) may cause either erroneous RNA expression or RNA degradation. Unfortunately, I don't have much knowledge about the time course of mRNA synthesis and degradation, so it's difficult for me to decide if such a timespan is significant. 2. For spinal or DRG tissue, is it necessary to use anything like proteinase K to make the membrane more permeable when using flourescent anti-sense probes, or is it OK just to hybridize as is. Any assistance would by greatly appreciated. Patrick Beall NeuroEngineering Laboratory School of Behavioral and Brain Sciences University of Texas at Dallas ________________________________________________________________________ Check Out the new free AIM(R) Mail -- 2 GB of storage and industry-leading spam and email virus protection. From Jason.PALMER <@t> svhm.org.au Thu Sep 7 18:40:04 2006 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Thu Sep 7 18:40:18 2006 Subject: [Histonet] re:anti mouse CD31 Message-ID: We have been using BD Biosciences rat anti mouse CD31 (Clone MEC13.3 / #553370) for a while now on FFPE mouse tissue (dermis and connective / adipose tissue mainly). On the whole, it works pretty well for us, though there is the odd tissue where labelling is very weak or absent and we can't quite seem to work out why. Nothing special about our protocol - proteinase K 8 mins, block, primary at 1:150, rabbit anti rat Ig - biotin, vector ABC elite, DAB.... Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ------------------------------ Message: 18 Date: Thu, 7 Sep 2006 10:46:15 -0500 From: "Jackie M O'Connor" Subject: [Histonet] Anti mouse CD31 To: histonet@pathology.swmed.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" If anyone has any new ideas on CD31 that works in murine tissue, I'll give you a million dollars. (If you'll take a check . . . . . post dated) Wacky Jackie - Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From palladineus <@t> yahoo.com Thu Sep 7 20:47:14 2006 From: palladineus <@t> yahoo.com (Randy Khoo) Date: Thu Sep 7 20:47:18 2006 Subject: [Histonet] Fontana Masson Staining Message-ID: <20060908014714.17543.qmail@web36503.mail.mud.yahoo.com> Dear All, I hope someone can help me out here. I read that alcohol will dissolve the argentaffin granules. How does the chemistry work here? Is it only because of beta-carboline formation or are there other reactions taking place as well? Is the disappearance of argentaffin granules under alcohol fixation confirmatory of its presence if compared to a parallel formaldehyde staining showing its presence? Also, if I use alcohol fixation, the cells tend to shrink and shrivel. Is there any way I can preserve the morphology with alcohol fixation (ie any other components I can add to alcohol)? Does Fontana-Masson staining pick up oxidized melanin? I presume its ability to reduce silver ions is because in the natural state, melanin is mostly in the reduced form. Therefore, is it possible that FM is not picking up all the melanin in a cell? However, am I right in assuming that formalin as a strong reducing agent would reduce all the melanin in melancytes if they are fixed in formalin? Thanks in advance. Randy __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From G.Spoelstra <@t> murdoch.edu.au Thu Sep 7 21:22:41 2006 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Thu Sep 7 21:22:49 2006 Subject: [Histonet] sections cutting thick and thin Message-ID: Dear Histonetters, Recently our Leica 2135 microtome has been giving us problems. It had only just been serviced and started to cut thick and thin. We've isolated it to the knife holder, as we exchanged the knife holder from a more recent Leica microtome and it was fine. Has anyone with this model had the same problem? Gerard Spoelstra Medical Scientist Murdoch University Western Australia From jnocito <@t> satx.rr.com Fri Sep 8 05:53:27 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 8 05:53:34 2006 Subject: [Histonet] new employer References: Message-ID: <007301c6d335$044c1420$63614542@yourxhtr8hvc4p> Janci, yes, y'all know I love Fridays. Everyone knows that Histotechs are hard to find and good ones are even harder. A manager who has their head on right will see what's going on. Unfortunately, some managers are placed in their position simply by default because they've been there longer with no training what so ever. It's sad because we spend at least 1/3 of our lives at work and it should be somewhat pleasant. People always carry heavy baggage with them. You don't how they were treated in the past. Some managers just treat employees bad because that's all they know. It's really sad when the doctors, lab director and HR don't do anything. I worked in a hospital like that and on my exit interview I had wrote four pages of concerns. It made me feel better a minor issue was addressed, but the major concerns were never addressed. I know that because I work for the same group of doctors that are affiliated with the hospital. Depending on your financial situation and the work force where you are at, I'd leave ASAP. Your health is more important. You can not constantly work under continued stress for the rest of you life. Good Luck Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Robyn Vazquez" To: ; ; Sent: Thursday, September 07, 2006 3:36 PM Subject: Re: [Histonet] new employer Janci, It is unfortunate that your manager doesn't see the discrepancies between the manual and the way things are done. Have you told your manager the differences and what they are telling you? Your manager should back you and take some action (update manual). Unfortunately, some managers I SAID SOME :>) don't care as long as the tissue gets out and inspections are past. I worked for a lab that I had two other co-workers harass, verbally abused (even to the supervisor), emotionally abused me and others and nothing was ever done. It even went as far as the Medical Director and HR. I felt my supervisor really let me down, I was the brunt of their abuse and she still did nothing to back me. I got another job. and happy now! Okay, I will stop, just venting. Have wonderful weekend Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.405 / Virus Database: 268.12.1/440 - Release Date: 9/6/2006 From gu.lang <@t> gmx.at Fri Sep 8 06:55:47 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 8 06:55:45 2006 Subject: AW: [Histonet] Fontana Masson Staining In-Reply-To: <20060908014714.17543.qmail@web36503.mail.mud.yahoo.com> Message-ID: <001101c6d33d$b8f15f90$eeeea8c0@SERVER01> We usually stain Fontana Masson on formalin fixed and paraffin embedded tissue. So there is also an alcoholstep because of dehydration. Melanocytes are stained well and show black pigment. You have to use neutral buffered formaldehyd, because without buffering formalinpigment may occur, that has also reducing activity on the silversolution. If all melanin in the tissue is stained or if there is a remaining unstained part is beyound my knowledge. hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Randy Khoo Gesendet: Freitag, 08. September 2006 03:47 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fontana Masson Staining Dear All, I hope someone can help me out here. I read that alcohol will dissolve the argentaffin granules. How does the chemistry work here? Is it only because of beta-carboline formation or are there other reactions taking place as well? Is the disappearance of argentaffin granules under alcohol fixation confirmatory of its presence if compared to a parallel formaldehyde staining showing its presence? Also, if I use alcohol fixation, the cells tend to shrink and shrivel. Is there any way I can preserve the morphology with alcohol fixation (ie any other components I can add to alcohol)? Does Fontana-Masson staining pick up oxidized melanin? I presume its ability to reduce silver ions is because in the natural state, melanin is mostly in the reduced form. Therefore, is it possible that FM is not picking up all the melanin in a cell? However, am I right in assuming that formalin as a strong reducing agent would reduce all the melanin in melancytes if they are fixed in formalin? Thanks in advance. Randy __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nathalie.dhomen <@t> icr.ac.uk Fri Sep 8 07:11:54 2006 From: nathalie.dhomen <@t> icr.ac.uk (ndhomen) Date: Fri Sep 8 07:12:20 2006 Subject: [Histonet] How to best treat mouse skin Message-ID: <804F8845-D729-4D98-BBE9-27C4024AC584@icr.ac.uk> Dear all, I have recently started as a postdoc at the institute of cancer research in London and will be working with a mouse model. I will be wanting to fix and section mouse skin and have been exploring the best ways to do this in the histonet archives. Various options seem to work, so I'm finding it hard to discover what has been most successful for people. I'm thinking of shaving the skin and fixing it in 4% paraformaldehyde although I'm not sure what length of time is appropriate. I have noticed people both cryosection and paraffin embed the skin and would be interested to hear if anyone prefers one over the other or whether one method is better for certain procedures than others, and if anyone has any optimised embedding protocols. All advice would be greatly appreciated as I've not worked with skin before. Oh and incidentally, if anyone knows how to extract DNA from fixed mouse skin that would be great, though I understand this forum is for histologists and I'm unlikely to get much comment on this point. Sincerely, Nathalie Dhomen From Kari.Zajic <@t> HCAhealthcare.com Fri Sep 8 07:26:33 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Fri Sep 8 07:26:39 2006 Subject: [Histonet] sections cutting thick and thin In-Reply-To: Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC651@ORLEV03.hca.corpad.net> Hi Gerard...yes, I have experienced that problem as well with my Leica Microtome. It seems that the "plates" that hold the back of the knife holder kind-of warp...I am sorry, I don't know the technical terms (my service guy always knew what I was referring to). They seem to warp after alot of cutting (I used to cut alot of uterus,bone,etc) and I would have them replaced about every 18 months or so or as soon as I experienced the thick/thin sections. I wish I could explain the part a little better for you!! If you take off the back plate of the knife holder, you'll see a small "square" with 2 thin metal clamps. Those are the culprits! Good luck, I know how frustrating they are!! Kari :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gerard Spoelstra Sent: Thursday, September 07, 2006 10:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections cutting thick and thin Dear Histonetters, Recently our Leica 2135 microtome has been giving us problems. It had only just been serviced and started to cut thick and thin. We've isolated it to the knife holder, as we exchanged the knife holder from a more recent Leica microtome and it was fine. Has anyone with this model had the same problem? Gerard Spoelstra Medical Scientist Murdoch University Western Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlm11 <@t> cornell.edu Fri Sep 8 08:15:00 2006 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Fri Sep 8 08:15:07 2006 Subject: [Histonet] free stuff Message-ID: <6.2.1.2.2.20060908090525.03cfdb68@postoffice9.mail.cornell.edu> Hi Everybody, Cleaning out.....Free: Tissue Tek Embedding Rings. Almost 1000. Leitz 1212 Microtome. Has been serviced...nothing guaranteed. Mettler H54 scale. Has been serviced...nothing guaranteed. I will pay for mailing the rings just to get rid of them. You will pay for shipping of the others. I will find out how to do that once someone says they want it. Thanks, Mary Lou Norman Cornell University Ithaca, NY 607-253-3047 From luke.perkocha <@t> ucsf.edu Fri Sep 8 08:42:06 2006 From: luke.perkocha <@t> ucsf.edu (Luke A Perkocha) Date: Fri Sep 8 08:42:17 2006 Subject: [Histonet] Control tissue for skin DIF Message-ID: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> Hello, I am trying to develop a protocol for doing direct immunofluorescence testing on skin for bullous disease. I have a couple of sample protocols but none say anything about positive control tissue. I'm uncomfortable running the test on clinical specimens and reporting out a negative, without good positive controls. Does anyone know of a commercial source for positive control tissue, or does anyone have some they would be willing to share for development? Help much appreciated. Thanks, Luke From vazquezr <@t> ohsu.edu Fri Sep 8 09:20:12 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 8 09:20:43 2006 Subject: [Histonet] new employer Message-ID: Joe, Well said!! Robyn From kfineout <@t> hotmail.com Fri Sep 8 09:42:21 2006 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Fri Sep 8 09:42:31 2006 Subject: [Histonet] CAP Microwave ?s Message-ID: I was wondering what most histology labs are doing about the new CAP microwave questions, especially the ventilation(ANP.29430) and temperature reproducibility(ANP.28290) ones??!! We are using a normal household microwave from Walmart. I looked into getting one made for lab use and it costs $1800 (I don't think so!!). I am now thinking about getting a fume hood, like the one we use for coverslipping slides, and put the microwave we have in that. Any suggestions?? Kelly Pathology Services of West MI From Jackie.O'Connor <@t> abbott.com Fri Sep 8 10:13:53 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 8 10:14:26 2006 Subject: [Histonet] zinc-fixed paraffin embedding tissue protocol In-Reply-To: <200609071916.k87JGS88018124@outgoing.mit.edu> Message-ID: When I have to use zinc buffer for 'fixation' of murine tissues, I use Streck Tissue Fixative. Harvested samples are bisected to no thicker than 3mm (like liver and tumors) prior to fixation, and allowed to fix in 20x volume for 24-48 hours. Samples should be rinsed well with 50% etoh prior to transfer to 70% etoh for storage or processing to avoid zinc precipitate, which will form if the fixative is not cleared from the tissues (particularly hemorrhagic tissues) prior to exposure to higher graded alchol. It's a messl. You can contact Streck Laboratories for more information. Some antigens are easier to retrieve in Streck fixed tissues, but the morphology is not as good as NBF. Further processing is the same as with NBF tissues - I usually do 45 minutes per station for murine tissue on a junky old open processor, and tissues come out fine. Jackie O' "Hector Mobine" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/07/2006 02:16 PM To cc Subject [Histonet] zinc-fixed paraffin embedding tissue protocol Hello Everyone, Does anyone have a good protocol for zinc fixing and paraffin embedding of animal tissue? I would greatly appreciate any help. Thanks, Hector ---------------------------------------------------------------------------- Langer Lab Biological Engineering Massachusetts Institute of Technology t: 617-258-8895 f: 617-253-2514 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Fri Sep 8 10:29:53 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Fri Sep 8 10:29:57 2006 Subject: [Histonet] Control tissue for skin DIF In-Reply-To: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> Message-ID: Luke, I had no idea even what this disease was and did and search sounds terrible. Any way there is a Registry "National Epidermolysis Bullosa Registry" you might contact to se if they have any samples. Stanford has a lab that does a panel of immunoflourescent studies so they might be helpful as well. Good Luck! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Luke A Perkocha [mailto:luke.perkocha@ucsf.edu] Sent: Friday, September 08, 2006 6:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control tissue for skin DIF Hello, I am trying to develop a protocol for doing direct immunofluorescence testing on skin for bullous disease. I have a couple of sample protocols but none say anything about positive control tissue. I'm uncomfortable running the test on clinical specimens and reporting out a negative, without good positive controls. Does anyone know of a commercial source for positive control tissue, or does anyone have some they would be willing to share for development? Help much appreciated. Thanks, Luke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Traczyk7 <@t> aol.com Fri Sep 8 10:36:17 2006 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Sep 8 10:36:24 2006 Subject: [Histonet] Decal End Point Message-ID: Would someone please supply me with contact information for a company that supplies a chemical that will assist in determining when a decal solution is depleted? Thanks, Dorothy From mlm11 <@t> cornell.edu Fri Sep 8 11:14:07 2006 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Fri Sep 8 11:14:13 2006 Subject: Fwd: [Histonet] No free stuff Message-ID: <6.2.1.2.2.20060908120851.03e61e60@postoffice9.mail.cornell.edu> Boy that was quick. Everything is gone. Thanks for your interest. Mary Lou >Hi Everybody, > >Cleaning out.....Free: > >Tissue Tek Embedding Rings. Almost 1000. > >Leitz 1212 Microtome. Has been serviced...nothing guaranteed. > >Mettler H54 scale. Has been serviced...nothing guaranteed. > >I will pay for mailing the rings just to get rid of them. > >You will pay for shipping of the others. I will find out how to do that >once someone says they want it. > >Thanks, >Mary Lou Norman >Cornell University >Ithaca, NY >607-253-3047 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Sep 8 11:44:19 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Sep 8 11:44:33 2006 Subject: [Histonet] Decal End Point Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017177A1@lsexch.lsmaster.lifespan.org> I'm not sure exactly what you mean. In the title of your message you referred to "decal end point", but in the message you said "when a decal solution is depleted". The "decal end point" of course would be the point where all the calcium has been removed from the tissue. This can be tested chemically. However, that isn't the same thing as depletion of the decal solution. Decal solutions do not ordinarily become depleted. In most cases, the active ingredient in the decal solution is many times the amount necessary to decalcify the specimens - assuming of course that you use a sufficiently large volume of decal solution. If you put 5 cc of bone into 10 cc of decal solution, then the solution might become depleted. An acid decal solution, if depleted, would theoretically become neutral in pH, but I have never seen this happen. I'm assuming that you are actually inquiring about determining the end point of decalcification. This can be done by taking 5 cc of the decal solution from the specimens in a test tube and adding 1 cc of 5% sodium oxalate. Mix well and let it stand about a minute. If it becomes cloudy, the cloudiness is due to the precipitation of insoluble calcium oxalate. Of course, after such a test the decal solution must be changed before another test can be done. When the solution remains clear, no further calcium is coming out of the tissue, so one can assume that decalcification is complete. Ammonium oxalate can also be used in place of sodium oxalate. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Traczyk7@aol.com > Sent: Friday, September 8, 2006 8:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decal End Point > > Would someone please supply me with contact information for a company that > > supplies a chemical that will assist in determining when a decal solution > is > depleted? > Thanks, > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JMcCormick <@t> schosp.org Fri Sep 8 12:10:41 2006 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Fri Sep 8 12:10:49 2006 Subject: [Histonet] Decal End Point In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE017177A1@lsexch.lsmaster.lifespan.org> Message-ID: <913FAC2B773C19488E26AE6572180FA50315DB74@exch01.schosp.org> All, I think they are talking about the point at which there are no more calcium ions liberated into the electrolyte solution (acid+H2O). If there was a meter on the bath, as in a plating tank, there would be a distinct end point signaled by a Change in the electrolytic process. This is ,in fact, the end point of a titration of the bone fixed calcium that has been converted into a soluble calcium salt. At some point in the past years I have seen an electrolytic "fast" bone decalcifyer that worked this way. Many things have gone over the work bench of the years, only to be discovered or reinvented ! J.B.McCormick M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, September 08, 2006 10:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Decal End Point I'm not sure exactly what you mean. In the title of your message you referred to "decal end point", but in the message you said "when a decal solution is depleted". The "decal end point" of course would be the point where all the calcium has been removed from the tissue. This can be tested chemically. However, that isn't the same thing as depletion of the decal solution. Decal solutions do not ordinarily become depleted. In most cases, the active ingredient in the decal solution is many times the amount necessary to decalcify the specimens - assuming of course that you use a sufficiently large volume of decal solution. If you put 5 cc of bone into 10 cc of decal solution, then the solution might become depleted. An acid decal solution, if depleted, would theoretically become neutral in pH, but I have never seen this happen. I'm assuming that you are actually inquiring about determining the end point of decalcification. This can be done by taking 5 cc of the decal solution from the specimens in a test tube and adding 1 cc of 5% sodium oxalate. Mix well and let it stand about a minute. If it becomes cloudy, the cloudiness is due to the precipitation of insoluble calcium oxalate. Of course, after such a test the decal solution must be changed before another test can be done. When the solution remains clear, no further calcium is coming out of the tissue, so one can assume that decalcification is complete. Ammonium oxalate can also be used in place of sodium oxalate. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Traczyk7@aol.com > Sent: Friday, September 8, 2006 8:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decal End Point > > Would someone please supply me with contact information for a company that > > supplies a chemical that will assist in determining when a decal solution > is > depleted? > Thanks, > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From azsundi <@t> msn.com Fri Sep 8 18:59:28 2006 From: azsundi <@t> msn.com (Sundi Readlinger) Date: Fri Sep 8 18:59:36 2006 Subject: [Histonet] posting Message-ID: azsundi@msn.com From jnocito <@t> satx.rr.com Fri Sep 8 20:21:52 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 8 20:21:59 2006 Subject: [Histonet] Control tissue for skin DIF References: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> Message-ID: <009b01c6d3ae$55582c90$63614542@yourxhtr8hvc4p> Luke, if you find a source, please let me know. We save cases that stain positive, but we don't have a full spectrum of known controls. Our dermpath is content with that (no, really, this is a huge, huge point, this lady is not content with anything else). I've contacted several sources. They do not or will not commit to storing frozen section slides and still guarantee their antigenicity, which I can't blame them because our own controls fade after a while. I know this wasn't that helpful, but hell, it's Friday, so let's enjoy the weekend. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Luke A Perkocha" To: Sent: Friday, September 08, 2006 8:42 AM Subject: [Histonet] Control tissue for skin DIF > > Hello, > I am trying to develop a protocol for doing direct immunofluorescence > testing on skin for bullous disease. I have a couple of sample > protocols but none say anything about positive control tissue. I'm > uncomfortable running the test on clinical specimens and reporting out > a negative, without good positive controls. Does anyone know of a > commercial source for positive control tissue, or does anyone have > some they would be willing to share for development? Help much > appreciated. > Thanks, > Luke > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.12.2/442 - Release Date: 9/8/2006 > From jnocito <@t> satx.rr.com Fri Sep 8 20:30:18 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 8 20:30:27 2006 Subject: [Histonet] CAP Microwave ?s References: Message-ID: <00cd01c6d3af$82b5fc20$63614542@yourxhtr8hvc4p> Kelly, this is what we did. We purchased a tabletop fume hood from Mopec and a microwave leak detector from Lab Safety Supply (I think it was under $80). I looked at our range of times we used and set up a chart that had 15, 30 and 60 seconds with 3 rows each. I placed a beaker with distilled water and a calibrated thermometer and recorded the temps for three times. I used new water each time. At the time the microwave was on, I ran the meter all around the microwave and recorded the results. I didn't make it elaborate because I like the KISS method. It answers all the questions and it's recorded. I'd be willing to give you a copy of my procedures and charts. Just email me at work: jnocito@pathreflab.com Hopes this helps. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Kelly Larson" To: Sent: Friday, September 08, 2006 9:42 AM Subject: [Histonet] CAP Microwave ?s > > I was wondering what most histology labs are doing about the new CAP > microwave questions, especially the ventilation(ANP.29430) and > temperature reproducibility(ANP.28290) ones??!! We are using a normal > household microwave from Walmart. I looked into getting one made for > lab use and it costs $1800 (I don't think so!!). I am now thinking > about getting a fume hood, like the one we use for coverslipping > slides, and put the microwave we have in that. Any suggestions?? > > Kelly > > Pathology Services of West MI > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.12.2/442 - Release Date: 9/8/2006 > > From Traczyk7 <@t> aol.com Fri Sep 8 23:29:23 2006 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Sep 8 23:29:30 2006 Subject: [Histonet] Decal Message-ID: <4d3.64f432c.32339d23@aol.com> Okay...first, thanks to Pam Marcum of UPenn for directing me to a kit available from Polysciences. Sorry if my original subject line was misleading. What I want to do is figure out how long the decal solution continues to be effective. My client is happy with their technique of determining when the sample is sufficiently decalcified. Right now an adult rat femur is placed in a 50ml centrifuge tube with approximately 30 ml of EDTA. The tube is placed in a rack on a stirrer, the solution is changed once a week. I was thinking if they increased the volume of solution, or changed it more often, it would take less time to completely decalcify the sample. Am I on the right the track? Now I'm curious about the device that Dr. McCormick mentioned. Is anyone using it? Thanks, Dorothy From rjbuesa <@t> yahoo.com Sat Sep 9 09:22:24 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Sep 9 09:22:30 2006 Subject: [Histonet] Control tissue for skin DIF In-Reply-To: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> Message-ID: <20060909142224.32867.qmail@web61223.mail.yahoo.com> Luke: We always used for any skin disease tested (+) for DIF sections from (+) patients kept at -80?C Ren? J. Luke A Perkocha wrote: Hello, I am trying to develop a protocol for doing direct immunofluorescence testing on skin for bullous disease. I have a couple of sample protocols but none say anything about positive control tissue. I'm uncomfortable running the test on clinical specimens and reporting out a negative, without good positive controls. Does anyone know of a commercial source for positive control tissue, or does anyone have some they would be willing to share for development? Help much appreciated. Thanks, Luke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From nickandmanda <@t> paradise.net.nz Sun Sep 10 01:45:32 2006 From: nickandmanda <@t> paradise.net.nz (nick bowden) Date: Sun Sep 10 01:45:39 2006 Subject: [Histonet] Pressure cookers Message-ID: <20060910064533.4AD883F9C6@smtp-3.paradise.net.nz> Hello everyone!! We have come to the conclusion our old pressure cooker pot needs upgrading. could anyone please recommend a good pressure-cooker for antigen-retrieval methods and if so where to buy it? Thanx Amanda From jnocito <@t> satx.rr.com Sun Sep 10 09:09:41 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Sep 10 09:09:47 2006 Subject: [Histonet] Pressure cookers References: <20060910064533.4AD883F9C6@smtp-3.paradise.net.nz> Message-ID: <00b001c6d4e2$c2fbd820$63614542@yourxhtr8hvc4p> Amanda, contact Biocare Medical at 1-800-799-9949. They have one with really good safety features. They also have heat strips that you put inside when you A-R to ensure that the correct temp was reached. Hope this helps. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "nick bowden" To: Sent: Sunday, September 10, 2006 1:45 AM Subject: [Histonet] Pressure cookers > Hello everyone!! > > > > We have come to the conclusion our old pressure cooker pot needs > upgrading. > could anyone please recommend a good pressure-cooker for antigen-retrieval > methods and if so where to buy it? > > > > Thanx > > Amanda > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.12.2/442 - Release Date: 9/8/2006 > > From sheila_adey <@t> hotmail.com Sun Sep 10 11:15:11 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sun Sep 10 11:15:18 2006 Subject: [Histonet] Pressure cookers In-Reply-To: <00b001c6d4e2$c2fbd820$63614542@yourxhtr8hvc4p> Message-ID: Hi Amanda, Before you upgrade your pressure cooker, you may want to look into the EZ retriever (microwave) from Biogenex. We have a demo (that we are going to purchase). We have been getting much better looking tissue. We find it much more consistent with less variables. Our pressure cooker from Biocare is the kind that you turn the knob. Even filling the pot was a potential for different amounts of water being used. We don't have just one tech that does IHCs, so we are always trying to eliminate the potential for variables. Setting the microwave is pretty staight forward. Sheila Adey, HT MLT Port Huron Hospital >From: "Joe Nocito" >To: "nick bowden" >, >Subject: Re: [Histonet] Pressure cookers >Date: Sun, 10 Sep 2006 09:09:41 -0500 > >Amanda, >contact Biocare Medical at 1-800-799-9949. They have one with really good >safety features. They also have heat strips that you put inside when you >A-R to ensure that the correct temp was reached. Hope this helps. > >Joe Nocito BS, HT(ASCP)QIHC >Histology Manager >Pathology Reference Lab >San Antonio, TX >----- Original Message ----- From: "nick bowden" > >To: >Sent: Sunday, September 10, 2006 1:45 AM >Subject: [Histonet] Pressure cookers > > >>Hello everyone!! >> >> >> >>We have come to the conclusion our old pressure cooker pot needs >>upgrading. >>could anyone please recommend a good pressure-cooker for antigen-retrieval >>methods and if so where to buy it? >> >> >> >>Thanx >> >>Amanda >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>-- >>No virus found in this incoming message. >>Checked by AVG Free Edition. >>Version: 7.1.405 / Virus Database: 268.12.2/442 - Release Date: 9/8/2006 >> >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata=081805 From ladylaynah <@t> yahoo.com Sun Sep 10 22:12:04 2006 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Sun Sep 10 22:12:08 2006 Subject: [Histonet] help with MART-1 on frozen sections Message-ID: <20060911031204.75831.qmail@web37001.mail.mud.yahoo.com> Histo Landers! Hi! It's been over a year since I was on this list. I have had some very wild and hairy adventures setting up a business and now I'm back doing histology in a Moh's clinic at the University of Utah. I find this very exciting and really like it. It's waaaaay different from the veterinary stuff was doing for such a lont time. Anyway, I'm glad to be back. One of the docs wants to set up MART-1 for melanoma. If anyone out there is doing this on frozen sections, I would very much appreciate your advice on the questions below and anything else you might think of. 1) What do you fix your sections in? We routinely fix in acetone and I assume acetone is acceptable for IHC, also. Please verify if this is OK 2) I assume, but am seeking verification, that either a nonsense ab or buffer need to be applied to the test tissue and the pos ctrl tissue for a negative ctrl. 3) what do you rinse with? My procedure says to rinse with TRIS, but no mention of any surfactant (Triton X or Tween 20, etc). I'm inclined to add the surfactant, but would like verification if this is OK on frozens. 4) Who do you recommend purchasing ab's and ancilliary reagents from? Are there any RTU ab's out there that are reliable? 5) I have never worked with biotinylated abs before, but it seems to me they would eliminate the linking ab step, thus making the staining time shorter. thoughts?? What are some things to be aware of when using biotinylated abs? I think that's all for now. thanks bunches! Connie McManus HT __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From nathalie.dhomen <@t> icr.ac.uk Mon Sep 11 03:24:11 2006 From: nathalie.dhomen <@t> icr.ac.uk (ndhomen) Date: Mon Sep 11 03:24:19 2006 Subject: [Histonet] How to best treat mouse skin Message-ID: Dear all, thanks for your various responses, I now feel armed to try a couple of mouse skin fixing/embedding methods to see what works for me. You've been a great help, Nathalie From cpomajzl <@t> cpllabs.com Mon Sep 11 07:24:42 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Mon Sep 11 07:18:30 2006 Subject: [Histonet] Control tissue for skin DIF References: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> Message-ID: <004d01c6d59d$44738140$26fca8c0@CSP> We use fresh tonsil for +CTRL. We cut a tonsil down into many tiny pieces and freeze them in OCT. When doing DIF on skin, we will thaw one piece of tonsil and embed it in the same chuck as the skin specimen so that both specimens are on the same slide. It has worked very well since we started doing this. The pathologists love it. ----- Original Message ----- From: "Luke A Perkocha" To: Sent: Friday, September 08, 2006 8:42 AM Subject: [Histonet] Control tissue for skin DIF > > Hello, > I am trying to develop a protocol for doing direct immunofluorescence > testing on skin for bullous disease. I have a couple of sample > protocols but none say anything about positive control tissue. I'm > uncomfortable running the test on clinical specimens and reporting out > a negative, without good positive controls. Does anyone know of a > commercial source for positive control tissue, or does anyone have > some they would be willing to share for development? Help much > appreciated. > Thanks, > Luke > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Mon Sep 11 08:42:16 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Mon Sep 11 08:42:48 2006 Subject: [Histonet] Pressure cookers Message-ID: <45052168020000DF00001528@gwmail.medicine.wisc.edu> Amanda, I would second the Biocare Medical Decloaking Chamber. Tom Pier >>> nick bowden 09/10/06 1:45 AM >>> Hello everyone!! We have come to the conclusion our old pressure cooker pot needs upgrading. could anyone please recommend a good pressure-cooker for antigen-retrieval methods and if so where to buy it? Thanx Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Mon Sep 11 10:21:03 2006 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Mon Sep 11 10:21:12 2006 Subject: [Histonet] HT position available in VA Message-ID: <20060911152103.4450.qmail@web81006.mail.mud.yahoo.com> Netters, We have a full time HT position open in our histo lab here at Bon Secours Richmond hospital lab. Duties include: embedding, microtomy, special stains and IHC. This position can be applied for on-line. Please go to www.bonsecours.com to fill out an application and send your resume. Dawn Cowie, HT (ASCP) Director of Anatomic Pathology BSR From tfranks <@t> ma.rr.com Mon Sep 11 12:53:23 2006 From: tfranks <@t> ma.rr.com (Tina Franks) Date: Mon Sep 11 12:53:24 2006 Subject: [Histonet] Mohs technical error Message-ID: <003c01c6d5cb$2d85d220$fb7cd218@yourkybtg65gxe> Hello! I was in desperate need on some advice of frozen (skin) samples chipping out before complete section is obtained. Thank you, Tina F. Morgantown, WV From vazquezr <@t> ohsu.edu Mon Sep 11 13:52:30 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Sep 11 13:53:07 2006 Subject: [Histonet] Mohs technical error Message-ID: Tina, Are you freezing with liquid nitrogen? could be that you are freezing too long. just give small intermitted shots of liq nitro to set it up. I find that has helped in the past. Robyn OHSU From m-degutes <@t> northwestern.edu Mon Sep 11 14:31:40 2006 From: m-degutes <@t> northwestern.edu (Mathew DeGutes) Date: Mon Sep 11 14:31:46 2006 Subject: [Histonet] How to best treat mouse skin Message-ID: <20060911193140.4BA4035C75@casbah.it.northwestern.edu> In the past I have disected and sectioned mouse skin, and had perfect sections with a 1 hour fixation in 4% PFA on ice followed by 30% sucrose overnight at 4c and embedding in OCT. From lrichey <@t> u.washington.edu Mon Sep 11 17:57:38 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Mon Sep 11 17:57:49 2006 Subject: [Histonet] Control tissue for skin DIF In-Reply-To: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> References: <6.2.3.4.2.20060908064118.02f084c8@exchange.ucsf.edu> Message-ID: <4505E9E2.2000209@u.washington.edu> You can use normal fresh tonsil for G,A, and M, Placenta for Fibrinogen,, and for C3 and C1q, we use postive samples. Luke A Perkocha wrote: > Hello, > I am trying to develop a protocol for doing direct immunofluorescence > testing on skin for bullous disease. I have a couple of sample > protocols but none say anything about positive control tissue. I'm > uncomfortable running the test on clinical specimens and reporting out > a negative, without good positive controls. Does anyone know of a > commercial source for positive control tissue, or does anyone have > some they would be willing to share for development? Help much > appreciated. > Thanks, > Luke >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ohenry <@t> dfw.net Tue Sep 12 02:27:06 2006 From: ohenry <@t> dfw.net (Susan Owens) Date: Tue Sep 12 02:27:18 2006 Subject: [Histonet] Black Walnut stains Message-ID: <000601c6d63c$dab03280$c6f763d8@your4f1261a8e5> OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors From JWEEMS <@t> sjha.org Tue Sep 12 05:39:57 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Sep 12 05:40:17 2006 Subject: [Histonet] Black Walnut stains Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEA09@sjhaexc02.sjha.org> I don't know of any way, but I would try Eradasol if you have that in your lab. I didn't have that when I had walnut stained hands growing up! It will eventually wear off! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Tuesday, September 12, 2006 3:27 AM To: Histonet Subject: [Histonet] Black Walnut stains OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From hodges420 <@t> msn.com Tue Sep 12 06:39:21 2006 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Tue Sep 12 06:39:35 2006 Subject: [Histonet] tissues in cleaning cycle In-Reply-To: Message-ID: a tissue left in the cleaning cycle is completetly taben back to water and needs to be reprocessed for formalin. just place the tissue on ice water ans see how fast the tissue is white. it a test for fixation that ols techs used long ago to test processing if it is white in 20-30 secs there is no fixation. I would just reprocess the block and have before .it comes out great tere hodges ______________________________________________________________ From: "Anne Van Binsbergen" To: "Rene J Buesa" , "Till, Renee" , Subject: RE: [Histonet] tissues in cleaning cycle Date: Wed, 6 Sep 2006 17:28:45 +0400 >I totally disagree- my vip5's cleaning cycle does xylene then absolute and then has a water wash cycle. If I left a block in my wash cycle it would end up in water so I would certainly reprocess it from at least the 95% station!! If I put it straight into the xylene I would put water into my xylene!!! >Just my 5cents worth here in the desert >Annie > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: 06 September 2006 17:01 >To: Till, Renee; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] tissues in cleaning cycle > >Renée: > A tissue left in the cleaning cycle is one that is already dehydrated and in the "antemedium" (xylene if that is what you use). That is "normal procedure" if you want to "reinfiltrate" the tissue, so your next step would be to put it BRIEFLY in clean xylene and transfer it to be infiltrated with paraffin. > Very likely tat your tissue can be put in the last xylene station and through the paraffin stations. > René J. > >"Till, Renee" wrote: > Hello. Any suggestions on what to do with tissues that were accidentally >left in the processor during the cleaning cycle? I would think they need >to be re-infiltrated with paraffin at the very least. I don't know if >our processor will let us do anything but a full processing run. > > > >Renee' Till, HT > >Research Assistant > >Arkansas Children's Nutrition Center > >1212 Marshall St./N2021 > >Little Rock, AR 72002 > >Office (501)364-2785 > >Fax (501)364-3161 > > > > >Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Stay in the know. Pulse on the new Yahoo.com. Check it out. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Sep 12 06:34:03 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Sep 12 06:40:32 2006 Subject: [Histonet] Black Walnut stains References: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEA09@sjhaexc02.sjha.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A5EF@fhosxchmb006.ADVENTISTCORP.NET> My parents had a Black Walnut tree in our backyard and we never did find out how to remove those stains. Thinking back now though, you might not look so much like a walnut-stained piece of furniture if you use furniture stain remover (?). Good Luck with this one!! P.S. Read the warnings on the stain remover before using, they're extremely caustic! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Tue 9/12/2006 6:39 AM To: Susan Owens; Histonet Subject: RE: [Histonet] Black Walnut stains I don't know of any way, but I would try Eradasol if you have that in your lab. I didn't have that when I had walnut stained hands growing up! It will eventually wear off! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Tuesday, September 12, 2006 3:27 AM To: Histonet Subject: [Histonet] Black Walnut stains OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Sep 12 07:26:40 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Sep 12 07:26:44 2006 Subject: [Histonet] Black Walnut stains Message-ID: I have found nothing that will get rid of the black WALNUT STAINS. Similar stains from the husks of pecans. Just have o let it wear off. It is embarrassing as it gets under finger nails and looks as if you hav'nt cleaned your nails properly. You may want to go a step further and create a design with the juice and say that it is henna stain. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Tuesday, September 12, 2006 2:27 AM To: Histonet Subject: [Histonet] Black Walnut stains OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Sep 12 08:25:32 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Sep 12 08:25:37 2006 Subject: [Histonet] Adenovirus testing needed Message-ID: Hello, Are there any reference labs out there that perform adenovirus staining on FFPE sections by IHC? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From jcarpenter764 <@t> aol.com Tue Sep 12 09:11:39 2006 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Tue Sep 12 09:12:14 2006 Subject: [Histonet] Is there any way in the state of New York Message-ID: <8C8A48C8016D20E-EEC-2A21@webmail-md11.sysops.aol.com> Is there any way that you can get grandfathered in, in New York without having to take a exam. After 5 year of experience in the lab I heard that with your supervisor's approval you can become a certified HT. Can someone let me know anything about this...Thank you Jennell ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From abright <@t> brightinstruments.com Tue Sep 12 09:46:04 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Sep 12 09:33:33 2006 Subject: [Histonet] Mohs technical error Message-ID: Dear Tina, Is the skin sample pre-frozen prior to adding onto the embedding medium? In which case if the sample is too cold this could happen, and it would be better to place it into the embedding medium slightly earlier. Also if the freezing method is too fast this and cracking could occur. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Tina Franks [mailto:tfranks@ma.rr.com] Sent: 11 September 2006 18:53 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mohs technical error Hello! I was in desperate need on some advice of frozen (skin) samples chipping out before complete section is obtained. Thank you, Tina F. Morgantown, WV _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Tue Sep 12 11:27:54 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Sep 12 11:28:09 2006 Subject: [Histonet] True Blue chromogen Message-ID: I am in need of some clarification on information in Chris van der Loos book "Immunoenzyme Multiple Staining Methods". On page 45 in the chart he lists TMB HRP substrate as producing a green color. In KPL's catalog it lists TMB as blue and I talked to their tech service and they also said it turns blue. Do you have to do something to the TMB to turn it green or does it turn green automatically with IHC ? Are there any other chromogens that turn green? Thanks in advance for your help. Margaret Perry HT(ASCP) South Dakota State University Vet Science From dassog <@t> evergreen.edu Tue Sep 12 11:39:49 2006 From: dassog <@t> evergreen.edu (Dasso, Greg (staff)) Date: Tue Sep 12 11:41:44 2006 Subject: [Histonet] Black Walnut stains References: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEA09@sjhaexc02.sjha.org> Message-ID: <3872E8431D06E545AC955BED177CB6F6016049A8@oak.evergreen.edu> You just have to wait. It will come off eventually. Walnut husk is a tradional textile dye. now, if you could only label something with it. /Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Tue 9/12/2006 3:39 AM To: Susan Owens; Histonet Subject: RE: [Histonet] Black Walnut stains I don't know of any way, but I would try Eradasol if you have that in your lab. I didn't have that when I had walnut stained hands growing up! It will eventually wear off! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Tuesday, September 12, 2006 3:27 AM To: Histonet Subject: [Histonet] Black Walnut stains OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Sep 12 13:24:30 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Sep 12 13:25:47 2006 Subject: [Histonet] Black Walnut stains References: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEA09@sjhaexc02.sjha.org> <3872E8431D06E545AC955BED177CB6F6016049A8@oak.evergreen.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A5F9@fhosxchmb006.ADVENTISTCORP.NET> It would make a great histo lab marker!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg (staff) Sent: Tue 9/12/2006 12:39 PM To: Weems, Joyce; Susan Owens; Histonet Subject: RE: [Histonet] Black Walnut stains You just have to wait. It will come off eventually. Walnut husk is a tradional textile dye. now, if you could only label something with it. /Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Tue 9/12/2006 3:39 AM To: Susan Owens; Histonet Subject: RE: [Histonet] Black Walnut stains I don't know of any way, but I would try Eradasol if you have that in your lab. I didn't have that when I had walnut stained hands growing up! It will eventually wear off! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Tuesday, September 12, 2006 3:27 AM To: Histonet Subject: [Histonet] Black Walnut stains OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Sep 12 14:18:40 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Sep 12 14:19:18 2006 Subject: [Histonet] Black Walnut stains In-Reply-To: <5F31F38C96781A4FBE3196EBC22D478004A5F9@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: I have a beer dyed shirt from the "Ugly Tuna Saloona", and there are money dyed shirts, as well as red dirt dyed shirts from Hawaii. I know this has absolutely nothing to do with anything, but all the cool people are at the NSH...........(ha). "Bonner, Janet" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/12/2006 01:24 PM To "Dasso, Greg (staff)" , "Weems, Joyce" , "Susan Owens" , "Histonet" cc Subject RE: [Histonet] Black Walnut stains It would make a great histo lab marker!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg (staff) Sent: Tue 9/12/2006 12:39 PM To: Weems, Joyce; Susan Owens; Histonet Subject: RE: [Histonet] Black Walnut stains You just have to wait. It will come off eventually. Walnut husk is a tradional textile dye. now, if you could only label something with it. /Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Tue 9/12/2006 3:39 AM To: Susan Owens; Histonet Subject: RE: [Histonet] Black Walnut stains I don't know of any way, but I would try Eradasol if you have that in your lab. I didn't have that when I had walnut stained hands growing up! It will eventually wear off! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Owens Sent: Tuesday, September 12, 2006 3:27 AM To: Histonet Subject: [Histonet] Black Walnut stains OFF SUBJECT BUT I NEED HELP. Guys, I need some help... Yesterday I decided to peel and clean some Black Walnuts to plant later. Now I've never done this before....My walnut trees came from my father-in-law's place years ago, where the trees grow wild in the mountains. When the nuts, which look like hard green apples, fell from the trees he peeled the thick hard outer cover then cleaned and polish the inner nut(taste great)...We were there one Christmas and took several nuts home, planted them, and now have several beautiful big shade trees. After all these years I decided I needed more trees(lost several oaks) and I wanted to replace the oaks with the walnuts...........Sooooooo since they are falling now, a collected several and started the hard job of peeling.....Under that green outer skin you fine a light color(yellow-green) meat covering the nut.....I had peeled a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems the clear juice coming from that meat turns brown-dark brown after a while.....I have tried everything and nothing will touch it.......Surely someone out there knows what to do!!!! HELP!!!!! Thanks Susan, who should of wore gloves,but who knew. ohenry@dfw.net Ohenry Labradors _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KParker <@t> mail.nih.gov Tue Sep 12 14:23:05 2006 From: KParker <@t> mail.nih.gov (Tuttle, Kimberly (NIH/NCI) [E]) Date: Tue Sep 12 14:23:21 2006 Subject: [Histonet] Is there any way in the state of New York In-Reply-To: <8C8A48C8016D20E-EEC-2A21@webmail-md11.sysops.aol.com> Message-ID: I sure hope not! -----Original Message----- From: jcarpenter764@aol.com [mailto:jcarpenter764@aol.com] Sent: Tuesday, September 12, 2006 9:12 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Is there any way in the state of New York Is there any way that you can get grandfathered in, in New York without having to take a exam. After 5 year of experience in the lab I heard that with your supervisor's approval you can become a certified HT. Can someone let me know anything about this...Thank you Jennell ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> TriadHospitals.com Tue Sep 12 14:55:47 2006 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Tue Sep 12 14:55:57 2006 Subject: [Histonet] Surgical Pathology Departments Message-ID: <673832E27C45FC4D97EF758FFC777C270149299C@CPRTEVS01.triadhospitals.net> Hi Everyone, I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've missed in many years).... but, since I'm not, I have a question for all of you! Are their hospital based or even private practice anatomic pathology laboratories which operate without transcriptionists/secretary/clerical type individuals for reporting and distribution of reports, etc. and ALL those things they do? If so, who performs the duties that would normally be part of their job.... ?? Seems I vaguely remember a short thread about this maybe a year ago... Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com From histologyfield <@t> yahoo.com Tue Sep 12 14:18:01 2006 From: histologyfield <@t> yahoo.com (Histology Field) Date: Tue Sep 12 15:45:12 2006 Subject: [Histonet] BrdU control Message-ID: <20060912191801.683.qmail@web58613.mail.re3.yahoo.com> Hi there, I was wondering if anyone out there had any BrdU control tissue that they would be willing to share? I am working on a project that requires BrdU staining, but unfortunately, I wasn't given any control tissue. Baboon gut BrdU tissue would be great? Or any other control tissue from any species. Any help would be appreciated! Thanks! N --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From Barry.R.Rittman <@t> uth.tmc.edu Tue Sep 12 16:29:19 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Sep 12 16:29:26 2006 Subject: [Histonet] Surgical Pathology Departments Message-ID: Hi Robert You have just pressed the "not my job" button. I believe that this is a constant sore point with many histotechs. As in many labs, tasks that used to be regarded as "secretarial" are now carried out by the histotechs. in addition to actual histologic tissue preparation. While I believe that it is important to understand the various aspects of a pathology lab I feel that in many cases the extensive training that histotechs receive in the preparation of tissue is wasted in these "other tasks". This is not to detract from the skill required to be an accurate transcriber but I really feel that this is not what most histotecs. are really paid for. In our institute there are no "secretaries" but due to political correctness are known as "administrative assistants". However these are fewer in number than the original secretarial staff (they also do not carry out any histological techniques). These extra tasks for the histotech. are not usually reflected in job descriptions and rarely come with extra remuneration. However the good news is that histotechs. are not alone. Many faculty are typing their own memos and often carrying out numerous other tasks that were originally carried out by secretaries. I stand corrected, we have one secretary here. This was someone who we perfused and have preserved in the main entrance so that the image of a real secretary is retained for posterity (has a nice bronze plaque also). Believe that Joe N. would classify this as a potential flaming topic. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Tuesday, September 12, 2006 2:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical Pathology Departments Hi Everyone, I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've missed in many years).... but, since I'm not, I have a question for all of you! Are their hospital based or even private practice anatomic pathology laboratories which operate without transcriptionists/secretary/clerical type individuals for reporting and distribution of reports, etc. and ALL those things they do? If so, who performs the duties that would normally be part of their job.... ?? Seems I vaguely remember a short thread about this maybe a year ago... Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Tue Sep 12 16:43:14 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Sep 12 16:43:43 2006 Subject: [Histonet] BrdU control Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F98AE@NVCIEXCH02.NVCI.org> Wouldn't you just use the tissue that you have for the project as the control? If you injected BrdU into the animal before you sacrificed it, then it should stain positive (provided the tissue has dividing cells in it), so I'm thinking that you should be able to use the tissue for the project as the positive control and tissue from an animal not treated with BrdU as the negative control. Right? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Field Sent: Tuesday, September 12, 2006 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BrdU control Hi there, I was wondering if anyone out there had any BrdU control tissue that they would be willing to share? I am working on a project that requires BrdU staining, but unfortunately, I wasn't given any control tissue. Baboon gut BrdU tissue would be great? Or any other control tissue from any species. Any help would be appreciated! Thanks! N --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From mtarango <@t> nvcancer.org Tue Sep 12 16:56:05 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Sep 12 16:56:31 2006 Subject: [Histonet] Surgical Pathology Departments Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F98AF@NVCIEXCH02.NVCI.org> Dude, I'm totally doing a bunch of work that isn't actually in my job description. I'm figuring it'll pay off at some point, but so far it just seems like I have too much to do (including transcribing all the pathology reports myself). Luckily, our volume for clinical cases is really low, because doing more than a few reports in a day really takes up a too much of my time. The one good thing about having me do it, is that I catch a lot of mistakes. Because I work here (as opposed to working for the outside transcription service we hired in the past), I have access to more information about the case and I can double check to make sure that things are correct and accurate. When a report would come back from the transcription service it seems as if the pathologists wouldn't read more than the diagnosis. Working on-site with the pathologists, I can just ask one of them about something when it doesn't look right. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, September 12, 2006 2:29 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Surgical Pathology Departments Hi Robert You have just pressed the "not my job" button. I believe that this is a constant sore point with many histotechs. As in many labs, tasks that used to be regarded as "secretarial" are now carried out by the histotechs. in addition to actual histologic tissue preparation. While I believe that it is important to understand the various aspects of a pathology lab I feel that in many cases the extensive training that histotechs receive in the preparation of tissue is wasted in these "other tasks". This is not to detract from the skill required to be an accurate transcriber but I really feel that this is not what most histotecs. are really paid for. In our institute there are no "secretaries" but due to political correctness are known as "administrative assistants". However these are fewer in number than the original secretarial staff (they also do not carry out any histological techniques). These extra tasks for the histotech. are not usually reflected in job descriptions and rarely come with extra remuneration. However the good news is that histotechs. are not alone. Many faculty are typing their own memos and often carrying out numerous other tasks that were originally carried out by secretaries. I stand corrected, we have one secretary here. This was someone who we perfused and have preserved in the main entrance so that the image of a real secretary is retained for posterity (has a nice bronze plaque also). Believe that Joe N. would classify this as a potential flaming topic. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Tuesday, September 12, 2006 2:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical Pathology Departments Hi Everyone, I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've missed in many years).... but, since I'm not, I have a question for all of you! Are their hospital based or even private practice anatomic pathology laboratories which operate without transcriptionists/secretary/clerical type individuals for reporting and distribution of reports, etc. and ALL those things they do? If so, who performs the duties that would normally be part of their job.... ?? Seems I vaguely remember a short thread about this maybe a year ago... Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From djmr55 <@t> hotmail.com Tue Sep 12 18:09:14 2006 From: djmr55 <@t> hotmail.com (donna rossi) Date: Tue Sep 12 18:09:24 2006 Subject: [Histonet] pH of HIER Message-ID: Does anyone out there take the pH of their retrieval solutions ( CITRA, EDTA) after they have been diluted from the concentrated form? Is this necessary or should we just document the pH from the company for the concentrated Retrieval ? Thanks, Donna, SRHS From laurie.reilly <@t> jcu.edu.au Tue Sep 12 18:07:52 2006 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Tue Sep 12 18:12:17 2006 Subject: [Histonet] Black Walnut stains In-Reply-To: <000601c6d63c$dab03280$c6f763d8@your4f1261a8e5> Message-ID: <5.2.0.9.0.20060913090553.00c19348@mail.jcu.edu.au> Dear All, Old time timber workers used to use lemon juice and sugar to remove timber stains, maybe that is worth a try. Regards, Laurie. At 02:27 AM 12/09/2006 -0500, Susan Owens wrote: >OFF SUBJECT BUT I NEED HELP. >Guys, I need some help... >Yesterday I decided to peel and clean some Black Walnuts to plant later. Now >I've never done this before....My walnut trees came from my father-in-law's >place years ago, where the trees grow wild in the mountains. When the nuts, >which look like hard green apples, fell from the trees he peeled the thick >hard outer cover then cleaned and polish the inner nut(taste great)...We >were there one Christmas and took several nuts home, planted them, and now >have several beautiful big shade trees. After all these years I decided I >needed more trees(lost several oaks) and I wanted to replace the oaks with >the walnuts...........Sooooooo since they are falling now, a collected >several and started the hard job of peeling.....Under that green outer skin >you fine a light color(yellow-green) meat covering the nut.....I had peeled >a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems >the clear juice coming from that meat turns brown-dark brown after a >while.....I have tried everything and nothing will touch it.......Surely >someone out there knows what to do!!!! HELP!!!!! > >Thanks >Susan, who should of wore gloves,but who knew. >ohenry@dfw.net >Ohenry Labradors > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 From jnocito <@t> satx.rr.com Tue Sep 12 18:52:33 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 12 18:52:38 2006 Subject: [Histonet] Surgical Pathology Departments References: Message-ID: <00af01c6d6c6$84a01470$63614542@yourxhtr8hvc4p> Robert, believe it or not, they tried me out transcribing once. I was a bit perturbed. I was not having a good day when the transcriptionist called in sick. My doctor asked me to see if I could transcribe some stat cases. My response went something like this: "I gross, embed, cut, and stain. Now you want be to transcribe. Just give me the damn slides and I'll read and sign out those too" We now have three transcriptionists and a secretary who can fill in as needed. Unlike Barry's, our's is not stuffed, bronzed or dusty. She really is a big help. Now, Barry, what's this "flaming thing" you talk about? Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rittman, Barry R" To: Sent: Tuesday, September 12, 2006 4:29 PM Subject: RE: [Histonet] Surgical Pathology Departments Hi Robert You have just pressed the "not my job" button. I believe that this is a constant sore point with many histotechs. As in many labs, tasks that used to be regarded as "secretarial" are now carried out by the histotechs. in addition to actual histologic tissue preparation. While I believe that it is important to understand the various aspects of a pathology lab I feel that in many cases the extensive training that histotechs receive in the preparation of tissue is wasted in these "other tasks". This is not to detract from the skill required to be an accurate transcriber but I really feel that this is not what most histotecs. are really paid for. In our institute there are no "secretaries" but due to political correctness are known as "administrative assistants". However these are fewer in number than the original secretarial staff (they also do not carry out any histological techniques). These extra tasks for the histotech. are not usually reflected in job descriptions and rarely come with extra remuneration. However the good news is that histotechs. are not alone. Many faculty are typing their own memos and often carrying out numerous other tasks that were originally carried out by secretaries. I stand corrected, we have one secretary here. This was someone who we perfused and have preserved in the main entrance so that the image of a real secretary is retained for posterity (has a nice bronze plaque also). Believe that Joe N. would classify this as a potential flaming topic. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Tuesday, September 12, 2006 2:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical Pathology Departments Hi Everyone, I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've missed in many years).... but, since I'm not, I have a question for all of you! Are their hospital based or even private practice anatomic pathology laboratories which operate without transcriptionists/secretary/clerical type individuals for reporting and distribution of reports, etc. and ALL those things they do? If so, who performs the duties that would normally be part of their job.... ?? Seems I vaguely remember a short thread about this maybe a year ago... Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.405 / Virus Database: 268.12.3/445 - Release Date: 9/11/2006 From jnocito <@t> satx.rr.com Tue Sep 12 18:59:16 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 12 18:59:54 2006 Subject: [Histonet] Black Walnut stains References: Message-ID: <011801c6d6c7$7555c8b0$63614542@yourxhtr8hvc4p> wait a minute. I'm cool and I'm not at NSH. Now as for the stains. I have no idea how to remove them, but hey, it should wear off in a month or two, maybe three or four. Six the latest. Joe ----- Original Message ----- From: "Jackie M O'Connor" To: "Bonner, Janet" Cc: "Histonet" ; "Weems, Joyce" ; ; "Susan Owens" Sent: Tuesday, September 12, 2006 2:18 PM Subject: RE: [Histonet] Black Walnut stains >I have a beer dyed shirt from the "Ugly Tuna Saloona", and there are money > dyed shirts, as well as red dirt dyed shirts from Hawaii. > I know this has absolutely nothing to do with anything, but all the cool > people are at the NSH...........(ha). > > > > "Bonner, Janet" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 09/12/2006 01:24 PM > > To > "Dasso, Greg (staff)" , "Weems, Joyce" > , "Susan Owens" , "Histonet" > > cc > > Subject > RE: [Histonet] Black Walnut stains > > > > > > > It would make a great histo lab marker!!! > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg > (staff) > Sent: Tue 9/12/2006 12:39 PM > To: Weems, Joyce; Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > > > You just have to wait. It will come off eventually. Walnut husk is a > tradional textile dye. > > now, if you could only label something with it. > > /Greg > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce > Sent: Tue 9/12/2006 3:39 AM > To: Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > I don't know of any way, but I would try Eradasol if you have that in > your lab. I didn't have that when I had walnut stained hands growing up! > It will eventually wear off! > > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Owens > Sent: Tuesday, September 12, 2006 3:27 AM > To: Histonet > Subject: [Histonet] Black Walnut stains > > OFF SUBJECT BUT I NEED HELP. > Guys, I need some help... > Yesterday I decided to peel and clean some Black Walnuts to plant later. > Now I've never done this before....My walnut trees came from my > father-in-law's place years ago, where the trees grow wild in the > mountains. When the nuts, which look like hard green apples, fell from > the trees he peeled the thick hard outer cover then cleaned and polish > the inner nut(taste great)...We were there one Christmas and took > several nuts home, planted them, and now have several beautiful big > shade trees. After all these years I decided I needed more trees(lost > several oaks) and I wanted to replace the oaks with the > walnuts...........Sooooooo since they are falling now, a collected > several and started the hard job of peeling.....Under that green outer > skin you fine a light color(yellow-green) meat covering the nut.....I > had peeled a few when I saw that my hands were dirty....Well, it wasn't > dirt.....Seems the clear juice coming from that meat turns brown-dark > brown after a while.....I have tried everything and nothing will touch > it.......Surely someone out there knows what to do!!!! HELP!!!!! > > Thanks > Susan, who should of wore gloves,but who knew. > ohenry@dfw.net > Ohenry Labradors > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.12.3/445 - Release Date: 9/11/2006 > > From rcartun <@t> harthosp.org Tue Sep 12 20:26:45 2006 From: rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Sep 12 20:27:24 2006 Subject: [Histonet] Adenovirus testing needed Message-ID: <450726150200007700001E16@hcnwgwds01.hh.chs> Hi Linda: We do IHC for adenovirus. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sebree Linda A." 09/12/06 9:25 AM >>> Hello, Are there any reference labs out there that perform adenovirus staining on FFPE sections by IHC? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 13 08:12:10 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 13 08:12:18 2006 Subject: [Histonet] Surgical Pathology Departments In-Reply-To: Message-ID: <20060913131210.58382.qmail@web61225.mail.yahoo.com> Robert: Any laboratory manager that assigns clerical work to a histotech is a fool and a dumbhead! Who in his/her right mind will pay the HT salary rate to do clerical work? This is one of the most stupid ways of managing the laboratory complement. In the same way that certified HTs should not be routine staining or coverslipping instead of embedding, sectioning or completing special procedures, they even less should be doing clerical work. That is what clerks, "administrative assistantes" or laboratory assistants should be doing. If unfortunately you work in such an environment where your abilities and train are so underappreciated, start finding another workplace because it may get to the point when you are going to be required to take care of the lawn or the lavatories. Ren? J. "Rittman, Barry R" wrote: Hi Robert You have just pressed the "not my job" button. I believe that this is a constant sore point with many histotechs. As in many labs, tasks that used to be regarded as "secretarial" are now carried out by the histotechs. in addition to actual histologic tissue preparation. While I believe that it is important to understand the various aspects of a pathology lab I feel that in many cases the extensive training that histotechs receive in the preparation of tissue is wasted in these "other tasks". This is not to detract from the skill required to be an accurate transcriber but I really feel that this is not what most histotecs. are really paid for. In our institute there are no "secretaries" but due to political correctness are known as "administrative assistants". However these are fewer in number than the original secretarial staff (they also do not carry out any histological techniques). These extra tasks for the histotech. are not usually reflected in job descriptions and rarely come with extra remuneration. However the good news is that histotechs. are not alone. Many faculty are typing their own memos and often carrying out numerous other tasks that were originally carried out by secretaries. I stand corrected, we have one secretary here. This was someone who we perfused and have preserved in the main entrance so that the image of a real secretary is retained for posterity (has a nice bronze plaque also). Believe that Joe N. would classify this as a potential flaming topic. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Tuesday, September 12, 2006 2:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical Pathology Departments Hi Everyone, I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've missed in many years).... but, since I'm not, I have a question for all of you! Are their hospital based or even private practice anatomic pathology laboratories which operate without transcriptionists/secretary/clerical type individuals for reporting and distribution of reports, etc. and ALL those things they do? If so, who performs the duties that would normally be part of their job.... ?? Seems I vaguely remember a short thread about this maybe a year ago... Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Wed Sep 13 08:29:12 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 13 08:29:22 2006 Subject: [Histonet] pH of HIER In-Reply-To: Message-ID: <20060913132912.82207.qmail@web61219.mail.yahoo.com> Rossi: When a run does not "work" you should be able to troubleshoot. If you do not exactly know what has happened you cannot troubleshoot. If you did not know what the pH was, this could be part of the problem. This is why you should determine the pH How accurately you do that depends on what is available to you. At least a good pH paper with close scale will be helpful. Yes, you should determine the pH It is just a few seconds and a lot of peace of mind. Ren? J. donna rossi wrote: Does anyone out there take the pH of their retrieval solutions ( CITRA, EDTA) after they have been diluted from the concentrated form? Is this necessary or should we just document the pH from the company for the concentrated Retrieval ? Thanks, Donna, SRHS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From cmiller <@t> physlab.com Wed Sep 13 08:33:52 2006 From: cmiller <@t> physlab.com (Cheryl Miller) Date: Wed Sep 13 08:34:09 2006 Subject: [Histonet] Is there any way in the state of New York In-Reply-To: Message-ID: <000001c6d739$42acdad0$8b01a8c0@plab.local> I sure hope not! May I second that????? .............I worked freaking hard on my registry! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuttle, Kimberly (NIH/NCI) [E] Sent: Tuesday, September 12, 2006 2:23 PM To: jcarpenter764@aol.com; histonet@pathology.swmed.edu Subject: RE: [Histonet] Is there any way in the state of New York I sure hope not! -----Original Message----- From: jcarpenter764@aol.com [mailto:jcarpenter764@aol.com] Sent: Tuesday, September 12, 2006 9:12 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Is there any way in the state of New York Is there any way that you can get grandfathered in, in New York without having to take a exam. After 5 year of experience in the lab I heard that with your supervisor's approval you can become a certified HT. Can someone let me know anything about this...Thank you Jennell ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhorne <@t> upei.ca Wed Sep 13 09:32:18 2006 From: mhorne <@t> upei.ca (Margaret Horne) Date: Wed Sep 13 08:38:24 2006 Subject: [Histonet] Black Walnut stains In-Reply-To: References: <5F31F38C96781A4FBE3196EBC22D478004A5F9@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <4507DE31.25939.206A85@localhost> In PEI you can buy PEI Dirt Shirts .; t-shirts stained with PEI red mud. Great for mothers of kids who play in the mud. :-) As for the walnut stains, Eradasol would work I think , but if not try Avon's Skin So Soft. I use it to get paint off my hands , brushes and clothes. Doesn't seem to work on enamel paint but worked fine on an oil base I was using once. good luck , Margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada From tkngflght <@t> yahoo.com Wed Sep 13 09:18:19 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Sep 13 09:18:30 2006 Subject: [Histonet] A question about HT licensure in various states --which ones require it? In-Reply-To: <000001c6d739$42acdad0$8b01a8c0@plab.local> Message-ID: <20060913141819.65103.qmail@web50915.mail.yahoo.com> Hi all- Other states I am aware of that require registry for HTs are Rhode Island Nevada Florida --any others? I wrote to the state of NY a few months ago and requested the registry information. They sent me the information for MT/MLT and CT but nothing for HT. Could someone enlighten me to the NY regulations in plain language? Thanks in advance-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From vazquezr <@t> ohsu.edu Wed Sep 13 09:23:45 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Sep 13 09:24:14 2006 Subject: [Histonet] Surgical Pathology Departments Message-ID: Robert, We have an EPIC system that the doctor and nurses input pat's info and we have electronic medical records now. There is though the staff that types the letters to the referring doctor, reporting the procedure. They did away with our transcriptionist... Hope this helps, Robyn OHSU From joycefr <@t> frontiernet.net Wed Sep 13 10:02:39 2006 From: joycefr <@t> frontiernet.net (Joyce Friedland) Date: Wed Sep 13 09:58:52 2006 Subject: [Histonet] A question about HT licensure In-Reply-To: <20060913141819.65103.qmail@web50915.mail.yahoo.com> References: <20060913141819.65103.qmail@web50915.mail.yahoo.com> Message-ID: Hi All, I am by no means an expert on the subject of New York State Licensure but one key thing to know is that licensure is in no way connected to ASCP Registry. They are separate things entirely. There are a number of pathways to gaining a license in NYS. Some methods are based on "grandfathering", some are based on education requirements and some are based on an exam, which to my knowledge is yet to be written. Your best way to get all the info is to go to the following web site and print out everything available as you may need to read through it several times in order to figure out which is the pathway for your particular situation : WWW.OP.NYSED.GOV Your application needs to be in by 9/30/06 Hope this helps. Joyce Friedland Muhlbauer Derm-Path Lab Pittsford, NY 585-586-5166 From Amanda.Garcia <@t> TriadHospitals.com Wed Sep 13 11:39:12 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Wed Sep 13 11:39:20 2006 Subject: [Histonet] Histotech limits Message-ID: <8B63039C9DF4554C8FDBF31235F0E14801FC19DA@CPRTEVS02.triadhospitals.net> Could anyone help me out with a question that was asked today about there being a limit on number of slides one histotech should be able to prepare in a regular 8 hour day. That is of course if there is another person available to handle the constant interruptions that happen throughout the day. Thanks in advance for your help > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From Amanda.Garcia <@t> TriadHospitals.com Wed Sep 13 11:46:46 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Wed Sep 13 11:47:38 2006 Subject: [Histonet] Automatic coverslippers Message-ID: <8B63039C9DF4554C8FDBF31235F0E14801FC19EB@CPRTEVS02.triadhospitals.net> I have another question for the experts out there. How much time does an automatic coverlipper save the histotech? Thanks again > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From cgfields <@t> lexhealth.org Wed Sep 13 11:51:38 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Wed Sep 13 11:51:48 2006 Subject: [Histonet] NSH Message-ID: Hi Netters, Nice time at NSH.... and good turnout. IF anyone took pictures at the banquet I would really appreciate someone emailing me a few. I took my camera everywhere and then did not take it to the banquet. Since I do the newsletter for SC I would like to include a couple of the presentations in this months issue. Thank you in advance. Carole Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 (803) 936-8214 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From rjbuesa <@t> yahoo.com Wed Sep 13 11:58:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 13 11:58:42 2006 Subject: [Histonet] Histotech limits In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E14801FC19DA@CPRTEVS02.triadhospitals.net> Message-ID: <20060913165835.2205.qmail@web61214.mail.yahoo.com> Amy: The average for 45 laboratories with workloads from 600 to 116,000 surgical cases/year, are the following: 1- section = 31 blocks / hour = 248 blocks / 8 hours 2- slides = 2 / block = 496 slides / 8 hours 3- Time utilization rate = 65% which reduces the work output to 161 blocks and 322 slides per 8 hour shift. The ranges are wide. If you want I can send you a Workshop I presented at the last year convention of the Florida Society of Histotechnology. Ren? J. "Garcia, Amanda" wrote: Could anyone help me out with a question that was asked today about there being a limit on number of slides one histotech should be able to prepare in a regular 8 hour day. That is of course if there is another person available to handle the constant interruptions that happen throughout the day. Thanks in advance for your help > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From rjbuesa <@t> yahoo.com Wed Sep 13 12:00:41 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 13 12:00:49 2006 Subject: [Histonet] Automatic coverslippers In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E14801FC19EB@CPRTEVS02.triadhospitals.net> Message-ID: <20060913170041.92037.qmail@web61216.mail.yahoo.com> Amanda: A combination of automated coverslippers/stainers and lab assistants increase the HT average productivity 2.4 times Ren? J. "Garcia, Amanda" wrote: I have another question for the experts out there. How much time does an automatic coverlipper save the histotech? Thanks again > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From Anna.Yates <@t> se.amedd.army.mil Wed Sep 13 13:03:55 2006 From: Anna.Yates <@t> se.amedd.army.mil (Yates, Anna M Ms BACH) Date: Wed Sep 13 13:04:22 2006 Subject: [Histonet] subscribe Message-ID: <17B8554B178BCC42BABB83121934086CFC037D@amedmlsermc133> A co-worker of mine would like to be added to histonet. Thanks Timothy.neal@se.amedd.army.mil From MadaryJ <@t> MedImmune.com Wed Sep 13 13:09:03 2006 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Sep 13 13:09:07 2006 Subject: [Histonet] Slide prep numbers Message-ID: <7CAB706201F11843BD26AD516326F0C80166D078@MD1MS007.medimmune.com> The query was how many slides one can prepare, but that is vague to me, but I am interested in this. By prepare, in my lab preparing is trimming, processing, embedding, microtomy, staining and coverslipping. I honestly do not believe I could trim, process, embed, cut, stain and coverslip 248 per day. I could probably make a fortune in a private histolab with a couple of techs like that. Is it just cutting? From rjbuesa <@t> yahoo.com Wed Sep 13 14:00:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 13 14:00:39 2006 Subject: [Histonet] Slide prep numbers In-Reply-To: <7CAB706201F11843BD26AD516326F0C80166D078@MD1MS007.medimmune.com> Message-ID: <20060913190035.19302.qmail@web61219.mail.yahoo.com> Yes, just sectioning! Even other tasks as trimming, preparing the blocks to cut and slides etching are considered separate. Ren? J. "Madary, Joseph" wrote: The query was how many slides one can prepare, but that is vague to me, but I am interested in this. By prepare, in my lab preparing is trimming, processing, embedding, microtomy, staining and coverslipping. I honestly do not believe I could trim, process, embed, cut, stain and coverslip 248 per day. I could probably make a fortune in a private histolab with a couple of techs like that. Is it just cutting? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From portera <@t> msu.edu Wed Sep 13 14:59:45 2006 From: portera <@t> msu.edu (Amy Porter) Date: Wed Sep 13 14:58:53 2006 Subject: [Histonet] TUNEL Apoptosis on Decalcified Tissue Message-ID: <001601c6d76f$28c0e630$8e7a0923@HistoJJ> Is anyone out there doing Tunel for apoptosis on decalcified material? If you are and would be willing to share any modifications you made to the protocol to improve the results? Thanks in advance - struggling here using a kit!!! Not Good! Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu From jnocito <@t> satx.rr.com Wed Sep 13 16:39:51 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 13 16:39:58 2006 Subject: [Histonet] NSH References: Message-ID: <008d01c6d77d$258cb4e0$63614542@yourxhtr8hvc4p> go ahead, rub it in. I missed going this year, last year I developed Pedro the kidney stone. It's okay. I'm over not going to Phoenix this year. Totally. So what if people were sitting by the pool drinking margaritas while I was working. No problem. Like I said, I'm over NOT going this year. Totally forgotten. No hard feelings. So what if I couldn't go this year. It's fine. Really. Don't care. Nope, not one bit, etc, etc, etc Joe ----- Original Message ----- From: "Carole Fields" To: Sent: Wednesday, September 13, 2006 11:51 AM Subject: [Histonet] NSH > Hi Netters, > Nice time at NSH.... and good turnout. IF anyone took pictures at the > banquet I would really appreciate someone emailing me a few. I took my > camera everywhere and then did not take it to the banquet. Since I do the > newsletter for SC I would like to include a couple of the presentations > in > this months issue. > Thank you in advance. > Carole > > Carole Fields, HT,ASCP > Pathology Supervisor > Lexington Medical Center > 2720 Sunset Blvd. > W. Columbia, SC 29169 > > (803) 936-8214 > > > _________________________________ > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of > its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed above if one is provided. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.12.3/446 - Release Date: 9/12/2006 > > From fmoraes <@t> igc.gulbenkian.pt Thu Sep 14 04:32:29 2006 From: fmoraes <@t> igc.gulbenkian.pt (fmoraes@igc.gulbenkian.pt) Date: Thu Sep 14 04:32:41 2006 Subject: [Histonet] Tissue teck or gelatin embedding? In-Reply-To: <20060913165829.382236BC02A@igcns.igc.gulbenkian.pt> References: <20060913165829.382236BC02A@igcns.igc.gulbenkian.pt> Message-ID: <1158226349.450921ad63adf@webmail.igc.gulbenkian.pt> Hi I am using gelatin embedding to perform cryosections of E10.5-E11.5 mouse embryos and then I will use the sections for Immuno fluorescence protocols. However I notice some people include their samples only on tissue teck is there any difference can I use tissue teck to avoid the steps of gelatin/sucrose mouse embedding? Thanks Filipa Moraes Mois??s Mallo's Lab Neural Crest Group Instituto Gulbenkian de Ci??ncia Rua da Quinta Grande 6 2800-156, Oeiras Portugal URL: www.igc.gulbenkian.pt fmoraes@igc.gulbenkian.pt Phone: +351 214464525 Quoting histonet-request@lists.utsouthwestern.edu: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Black Walnut stains (Bonner, Janet) > 2. RE: Black Walnut stains (Jackie M O'Connor) > 3. RE: Is there any way in the state of New York > (Tuttle, Kimberly (NIH/NCI) [E]) > 4. Surgical Pathology Departments (Lott, Robert) > 5. BrdU control (Histology Field) > 6. RE: Surgical Pathology Departments (Rittman, Barry R) > 7. RE: BrdU control (Tarango, Mark) > 8. RE: Surgical Pathology Departments (Tarango, Mark) > 9. pH of HIER (donna rossi) > 10. Re: Black Walnut stains (Laurie Reilly) > 11. Re: Surgical Pathology Departments (Joe Nocito) > 12. Re: Black Walnut stains (Joe Nocito) > 13. Re: Adenovirus testing needed (Richard Cartun) > 14. RE: Surgical Pathology Departments (Rene J Buesa) > 15. Re: pH of HIER (Rene J Buesa) > 16. RE: Is there any way in the state of New York (Cheryl Miller) > 17. RE: Black Walnut stains (Margaret Horne) > 18. A question about HT licensure in various states --which ones > require it? (Cheryl) > 19. Re: Surgical Pathology Departments (Robyn Vazquez) > 20. Re: A question about HT licensure (Joyce Friedland) > 21. Histotech limits (Garcia, Amanda) > 22. Automatic coverslippers (Garcia, Amanda) > 23. NSH (Carole Fields) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 12 Sep 2006 14:24:30 -0400 > From: "Bonner, Janet" > Subject: RE: [Histonet] Black Walnut stains > To: "Dasso, Greg (staff)" , "Weems, Joyce" > , "Susan Owens" , "Histonet" > > Message-ID: > <5F31F38C96781A4FBE3196EBC22D478004A5F9@fhosxchmb006.ADVENTISTCORP.NET> > > Content-Type: text/plain; charset=iso-8859-1 > > It would make a great histo lab marker!!! > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg > (staff) > Sent: Tue 9/12/2006 12:39 PM > To: Weems, Joyce; Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > > > You just have to wait. It will come off eventually. Walnut husk is a > tradional textile dye. > > now, if you could only label something with it. > > /Greg > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce > Sent: Tue 9/12/2006 3:39 AM > To: Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > I don't know of any way, but I would try Eradasol if you have that in > your lab. I didn't have that when I had walnut stained hands growing up! > It will eventually wear off! > > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Owens > Sent: Tuesday, September 12, 2006 3:27 AM > To: Histonet > Subject: [Histonet] Black Walnut stains > > OFF SUBJECT BUT I NEED HELP. > Guys, I need some help... > Yesterday I decided to peel and clean some Black Walnuts to plant later. > Now I've never done this before....My walnut trees came from my > father-in-law's place years ago, where the trees grow wild in the > mountains. When the nuts, which look like hard green apples, fell from > the trees he peeled the thick hard outer cover then cleaned and polish > the inner nut(taste great)...We were there one Christmas and took > several nuts home, planted them, and now have several beautiful big > shade trees. After all these years I decided I needed more trees(lost > several oaks) and I wanted to replace the oaks with the > walnuts...........Sooooooo since they are falling now, a collected > several and started the hard job of peeling.....Under that green outer > skin you fine a light color(yellow-green) meat covering the nut.....I > had peeled a few when I saw that my hands were dirty....Well, it wasn't > dirt.....Seems the clear juice coming from that meat turns brown-dark > brown after a while.....I have tried everything and nothing will touch > it.......Surely someone out there knows what to do!!!! HELP!!!!! > > Thanks > Susan, who should of wore gloves,but who knew. > ohenry@dfw.net > Ohenry Labradors > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, distribution > or the taking of any action in reliance on the contents of this information > is strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. Thank you. Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Tue, 12 Sep 2006 14:18:40 -0500 > From: "Jackie M O'Connor" > Subject: RE: [Histonet] Black Walnut stains > To: "Bonner, Janet" > Cc: Histonet , "Weems, Joyce" > , histonet-bounces@lists.utsouthwestern.edu, Susan > Owens > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I have a beer dyed shirt from the "Ugly Tuna Saloona", and there are money > dyed shirts, as well as red dirt dyed shirts from Hawaii. > I know this has absolutely nothing to do with anything, but all the cool > people are at the NSH...........(ha). > > > > "Bonner, Janet" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 09/12/2006 01:24 PM > > To > "Dasso, Greg (staff)" , "Weems, Joyce" > , "Susan Owens" , "Histonet" > > cc > > Subject > RE: [Histonet] Black Walnut stains > > > > > > > It would make a great histo lab marker!!! > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg > (staff) > Sent: Tue 9/12/2006 12:39 PM > To: Weems, Joyce; Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > > > You just have to wait. It will come off eventually. Walnut husk is a > tradional textile dye. > > now, if you could only label something with it. > > /Greg > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce > Sent: Tue 9/12/2006 3:39 AM > To: Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > I don't know of any way, but I would try Eradasol if you have that in > your lab. I didn't have that when I had walnut stained hands growing up! > It will eventually wear off! > > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Owens > Sent: Tuesday, September 12, 2006 3:27 AM > To: Histonet > Subject: [Histonet] Black Walnut stains > > OFF SUBJECT BUT I NEED HELP. > Guys, I need some help... > Yesterday I decided to peel and clean some Black Walnuts to plant later. > Now I've never done this before....My walnut trees came from my > father-in-law's place years ago, where the trees grow wild in the > mountains. When the nuts, which look like hard green apples, fell from > the trees he peeled the thick hard outer cover then cleaned and polish > the inner nut(taste great)...We were there one Christmas and took > several nuts home, planted them, and now have several beautiful big > shade trees. After all these years I decided I needed more trees(lost > several oaks) and I wanted to replace the oaks with the > walnuts...........Sooooooo since they are falling now, a collected > several and started the hard job of peeling.....Under that green outer > skin you fine a light color(yellow-green) meat covering the nut.....I > had peeled a few when I saw that my hands were dirty....Well, it wasn't > dirt.....Seems the clear juice coming from that meat turns brown-dark > brown after a while.....I have tried everything and nothing will touch > it.......Surely someone out there knows what to do!!!! HELP!!!!! > > Thanks > Susan, who should of wore gloves,but who knew. > ohenry@dfw.net > Ohenry Labradors > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 12 Sep 2006 15:23:05 -0400 > From: "Tuttle, Kimberly \(NIH/NCI\) [E]" > Subject: RE: [Histonet] Is there any way in the state of New York > To: , > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I sure hope not! > > -----Original Message----- > From: jcarpenter764@aol.com [mailto:jcarpenter764@aol.com] > Sent: Tuesday, September 12, 2006 9:12 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Is there any way in the state of New York > > > Is there any way that you can get grandfathered in, in New York without > having to take a exam. After 5 year of experience in the lab I heard > that with your supervisor's approval you can become a certified HT. Can > someone let me know anything about this...Thank you Jennell > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and > security tools, free access to millions of high-quality videos from > across the web, free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Tue, 12 Sep 2006 14:55:47 -0500 > From: "Lott, Robert" > Subject: [Histonet] Surgical Pathology Departments > To: > Message-ID: > <673832E27C45FC4D97EF758FFC777C270149299C@CPRTEVS01.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 12 Sep 2006 12:18:01 -0700 (PDT) > From: Histology Field > Subject: [Histonet] BrdU control > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060912191801.683.qmail@web58613.mail.re3.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi there, > > I was wondering if anyone out there had any BrdU control tissue that they > would be willing to share? I am working on a project that requires BrdU > staining, but unfortunately, I wasn't given any control tissue. Baboon gut > BrdU tissue would be great? Or any other control tissue from any species. > Any help would be appreciated! > > Thanks! > N > > > --------------------------------- > Stay in the know. Pulse on the new Yahoo.com. Check it out. > > ------------------------------ > > Message: 6 > Date: Tue, 12 Sep 2006 16:29:19 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] Surgical Pathology Departments > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Tue, 12 Sep 2006 14:43:14 -0700 > From: "Tarango, Mark" > Subject: RE: [Histonet] BrdU control > To: "Histology Field" , > histonet@lists.utsouthwestern.edu > Message-ID: > <5AEC610C1CE02945BD63A395BA763EDE1F98AE@NVCIEXCH02.NVCI.org> > Content-Type: text/plain; charset=us-ascii > > Wouldn't you just use the tissue that you have for the project as the > control? If you injected BrdU into the animal before you sacrificed it, > then it should stain positive (provided the tissue has dividing cells in > it), so I'm thinking that you should be able to use the tissue for the > project as the positive control and tissue from an animal not treated > with BrdU as the negative control. Right? > > > > Mark Adam Tarango HT(ASCP) > > Histology/Immunohistochemistry Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > mtarango@nvcancer.org > > Direct Line (702) 822-5112 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Histology Field > Sent: Tuesday, September 12, 2006 12:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] BrdU control > > Hi there, > > I was wondering if anyone out there had any BrdU control tissue that > they would be willing to share? I am working on a project that requires > BrdU staining, but unfortunately, I wasn't given any control tissue. > Baboon gut BrdU tissue would be great? Or any other control tissue > from any species. Any help would be appreciated! > > Thanks! > N > > > --------------------------------- > Stay in the know. Pulse on the new Yahoo.com. Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "MMS " made the following annotations. > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential, > proprietary, and/or privileged information protected by law. If you are not > the intended recipient, you may not use, copy, or distribute this e-mail > message or its attachments. If you believe you have received this e-mail > message in error, please contact the sender by reply e-mail and destroy all > copies of the original message > ============================================================================== > > > > > ------------------------------ > > Message: 8 > Date: Tue, 12 Sep 2006 14:56:05 -0700 > From: "Tarango, Mark" > Subject: RE: [Histonet] Surgical Pathology Departments > To: "Rittman, Barry R" , > histonet@lists.utsouthwestern.edu > Message-ID: > <5AEC610C1CE02945BD63A395BA763EDE1F98AF@NVCIEXCH02.NVCI.org> > Content-Type: text/plain; charset=us-ascii > > Dude, I'm totally doing a bunch of work that isn't actually in my job > description. I'm figuring it'll pay off at some point, but so far it > just seems like I have too much to do (including transcribing all the > pathology reports myself). Luckily, our volume for clinical cases is > really low, because doing more than a few reports in a day really takes > up a too much of my time. > > The one good thing about having me do it, is that I catch a lot of > mistakes. Because I work here (as opposed to working for the outside > transcription service we hired in the past), I have access to more > information about the case and I can double check to make sure that > things are correct and accurate. When a report would come back from the > transcription service it seems as if the pathologists wouldn't read more > than the diagnosis. Working on-site with the pathologists, I can just > ask one of them about something when it doesn't look right. > > > > > > Mark Adam Tarango HT(ASCP) > > Histology/Immunohistochemistry Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > mtarango@nvcancer.org > > Direct Line (702) 822-5112 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, > Barry R > Sent: Tuesday, September 12, 2006 2:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Surgical Pathology Departments > > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "MMS " made the following annotations. > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential, > proprietary, and/or privileged information protected by law. If you are not > the intended recipient, you may not use, copy, or distribute this e-mail > message or its attachments. If you believe you have received this e-mail > message in error, please contact the sender by reply e-mail and destroy all > copies of the original message > ============================================================================== > > > > > ------------------------------ > > Message: 9 > Date: Tue, 12 Sep 2006 19:09:14 -0400 > From: "donna rossi" > Subject: [Histonet] pH of HIER > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > Does anyone out there take the pH of their retrieval > solutions ( CITRA, EDTA) after they have been diluted from the > concentrated form? Is this necessary or should we just document the > pH from the company for the concentrated Retrieval ? Thanks, Donna, > SRHS > > > ------------------------------ > > Message: 10 > Date: Wed, 13 Sep 2006 09:07:52 +1000 > From: Laurie Reilly > Subject: Re: [Histonet] Black Walnut stains > To: "Susan Owens" , "Histonet" > > Message-ID: <5.2.0.9.0.20060913090553.00c19348@mail.jcu.edu.au> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Dear All, > Old time timber workers used to use lemon juice and sugar to remove timber > stains, maybe that is worth a try. > > Regards, Laurie. > > > At 02:27 AM 12/09/2006 -0500, Susan Owens wrote: > >OFF SUBJECT BUT I NEED HELP. > >Guys, I need some help... > >Yesterday I decided to peel and clean some Black Walnuts to plant later. Now > >I've never done this before....My walnut trees came from my father-in-law's > >place years ago, where the trees grow wild in the mountains. When the nuts, > >which look like hard green apples, fell from the trees he peeled the thick > >hard outer cover then cleaned and polish the inner nut(taste great)...We > >were there one Christmas and took several nuts home, planted them, and now > >have several beautiful big shade trees. After all these years I decided I > >needed more trees(lost several oaks) and I wanted to replace the oaks with > >the walnuts...........Sooooooo since they are falling now, a collected > >several and started the hard job of peeling.....Under that green outer skin > >you fine a light color(yellow-green) meat covering the nut.....I had peeled > >a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems > >the clear juice coming from that meat turns brown-dark brown after a > >while.....I have tried everything and nothing will touch it.......Surely > >someone out there knows what to do!!!! HELP!!!!! > > > >Thanks > >Susan, who should of wore gloves,but who knew. > >ohenry@dfw.net > >Ohenry Labradors > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Mr.Laurie Reilly > School of Veterinary and Biomedical Sciences > James Cook University > Townsville. 4811 > Australia. > > Phone 07 4781 4468 > Fax 07 4779 1526 > > > > > ------------------------------ > > Message: 11 > Date: Tue, 12 Sep 2006 18:52:33 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Surgical Pathology Departments > To: "Rittman, Barry R" , > > Message-ID: <00af01c6d6c6$84a01470$63614542@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Robert, > believe it or not, they tried me out transcribing once. I was a bit > perturbed. I was not having a good day when the transcriptionist called in > sick. My doctor asked me to see if I could transcribe some stat cases. My > response went something like this: "I gross, embed, cut, and stain. Now you > want be to transcribe. Just give me the damn slides and I'll read and sign > out those too" > We now have three transcriptionists and a secretary who can fill in as > needed. Unlike Barry's, our's is not stuffed, bronzed or dusty. She really > is a big help. > Now, Barry, what's this "flaming thing" you talk about? > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > ----- Original Message ----- > From: "Rittman, Barry R" > To: > Sent: Tuesday, September 12, 2006 4:29 PM > Subject: RE: [Histonet] Surgical Pathology Departments > > > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.12.3/445 - Release Date: 9/11/2006 > > > > > > ------------------------------ > > Message: 12 > Date: Tue, 12 Sep 2006 18:59:16 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Black Walnut stains > To: "Jackie M O'Connor" , "Bonner, Janet" > > Cc: Histonet , > histonet-bounces@lists.utsouthwestern.edu, "Weems, Joyce" > , Susan Owens > Message-ID: <011801c6d6c7$7555c8b0$63614542@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > wait a minute. I'm cool and I'm not at NSH. > Now as for the stains. I have no idea how to remove them, but hey, it should > wear off in a month or two, maybe three or four. Six the latest. > > Joe > ----- Original Message ----- > From: "Jackie M O'Connor" > To: "Bonner, Janet" > Cc: "Histonet" ; "Weems, Joyce" > ; ; "Susan > Owens" > Sent: Tuesday, September 12, 2006 2:18 PM > Subject: RE: [Histonet] Black Walnut stains > > > >I have a beer dyed shirt from the "Ugly Tuna Saloona", and there are money > > dyed shirts, as well as red dirt dyed shirts from Hawaii. > > I know this has absolutely nothing to do with anything, but all the cool > > people are at the NSH...........(ha). > > > > > > > > "Bonner, Janet" > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 09/12/2006 01:24 PM > > > > To > > "Dasso, Greg (staff)" , "Weems, Joyce" > > , "Susan Owens" , "Histonet" > > > > cc > > > > Subject > > RE: [Histonet] Black Walnut stains > > > > > > > > > > > > > > It would make a great histo lab marker!!! > > > > ________________________________ > > > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg > > (staff) > > Sent: Tue 9/12/2006 12:39 PM > > To: Weems, Joyce; Susan Owens; Histonet > > Subject: RE: [Histonet] Black Walnut stains > > > > > > > > You just have to wait. It will come off eventually. Walnut husk is a > > tradional textile dye. > > > > now, if you could only label something with it. > > > > /Greg > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce > > Sent: Tue 9/12/2006 3:39 AM > > To: Susan Owens; Histonet > > Subject: RE: [Histonet] Black Walnut stains > > > > I don't know of any way, but I would try Eradasol if you have that in > > your lab. I didn't have that when I had walnut stained hands growing up! > > It will eventually wear off! > > > > Joyce > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > > Owens > > Sent: Tuesday, September 12, 2006 3:27 AM > > To: Histonet > > Subject: [Histonet] Black Walnut stains > > > > OFF SUBJECT BUT I NEED HELP. > > Guys, I need some help... > > Yesterday I decided to peel and clean some Black Walnuts to plant later. > > Now I've never done this before....My walnut trees came from my > > father-in-law's place years ago, where the trees grow wild in the > > mountains. When the nuts, which look like hard green apples, fell from > > the trees he peeled the thick hard outer cover then cleaned and polish > > the inner nut(taste great)...We were there one Christmas and took > > several nuts home, planted them, and now have several beautiful big > > shade trees. After all these years I decided I needed more trees(lost > > several oaks) and I wanted to replace the oaks with the > > walnuts...........Sooooooo since they are falling now, a collected > > several and started the hard job of peeling.....Under that green outer > > skin you fine a light color(yellow-green) meat covering the nut.....I > > had peeled a few when I saw that my hands were dirty....Well, it wasn't > > dirt.....Seems the clear juice coming from that meat turns brown-dark > > brown after a while.....I have tried everything and nothing will touch > > it.......Surely someone out there knows what to do!!!! HELP!!!!! > > > > Thanks > > Susan, who should of wore gloves,but who knew. > > ohenry@dfw.net > > Ohenry Labradors > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Confidentiality Notice ** The information contained in this message may be > > privileged and is confidential information intended for the use of the > > addressee listed above. If you are neither the intended recipient nor the > > employee or agent responsible for delivering this message to the intended > > recipient, you are hereby notified that any disclosure, copying, > > distribution or the taking of any action in reliance on the contents of > > this information is strictly prohibited. If you have received this > > communication in error, please notify us immediately by replying to the > > message and deleting it from your computer. Thank you. Saint Joseph's > > Health System, Inc. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > No virus found in this incoming message. > > Checked by AVG Free Edition. > > Version: 7.1.405 / Virus Database: 268.12.3/445 - Release Date: 9/11/2006 > > > > > > > > > ------------------------------ > > Message: 13 > Date: Tue, 12 Sep 2006 21:26:45 -0400 > From: "Richard Cartun" > Subject: Re: [Histonet] Adenovirus testing needed > To: , > Message-ID: <450726150200007700001E16@hcnwgwds01.hh.chs> > Content-Type: text/plain; charset=US-ASCII > > Hi Linda: > > We do IHC for adenovirus. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>> "Sebree Linda A." 09/12/06 9:25 AM >>> > Hello, > > Are there any reference labs out there that perform adenovirus staining > on FFPE sections by IHC? > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > ------------------------------ > > Message: 14 > Date: Wed, 13 Sep 2006 06:12:10 -0700 (PDT) > From: Rene J Buesa > Subject: RE: [Histonet] Surgical Pathology Departments > To: "Rittman, Barry R" , > histonet@lists.utsouthwestern.edu > Message-ID: <20060913131210.58382.qmail@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Robert: > Any laboratory manager that assigns clerical work to a histotech is a fool > and a dumbhead! > Who in his/her right mind will pay the HT salary rate to do clerical work? > This is one of the most stupid ways of managing the laboratory complement. > In the same way that certified HTs should not be routine staining or > coverslipping instead of embedding, sectioning or completing special > procedures, they even less should be doing clerical work. > That is what clerks, "administrative assistantes" or laboratory assistants > should be doing. > If unfortunately you work in such an environment where your abilities and > train are so underappreciated, start finding another workplace because it may > get to the point when you are going to be required to take care of the lawn > or the lavatories. > Ren??? J. > > "Rittman, Barry R" wrote: > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > How low will we go? Check out Yahoo! Messenger???s low PC-to-Phone call rates. > > ------------------------------ > > Message: 15 > Date: Wed, 13 Sep 2006 06:29:12 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] pH of HIER > To: donna rossi , > histonet@lists.utsouthwestern.edu > Message-ID: <20060913132912.82207.qmail@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Rossi: > When a run does not "work" you should be able to troubleshoot. If you do > not exactly know what has happened you cannot troubleshoot. > If you did not know what the pH was, this could be part of the problem. > This is why you should determine the pH How accurately you do that depends > on what is available to you. > At least a good pH paper with close scale will be helpful. > Yes, you should determine the pH It is just a few seconds and a lot of > peace of mind. > Ren??? J. > > donna rossi wrote: > > Does anyone out there take the pH of their retrieval > solutions ( CITRA, EDTA) after they have been diluted from the > concentrated form? Is this necessary or should we just document the > pH from the company for the concentrated Retrieval ? Thanks, Donna, > SRHS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates > starting at 1¢/min. > > ------------------------------ > > Message: 16 > Date: Wed, 13 Sep 2006 08:33:52 -0500 > From: "Cheryl Miller" > Subject: RE: [Histonet] Is there any way in the state of New York > To: "'Tuttle, Kimberly \(NIH/NCI\) [E]'" , > , > Message-ID: <000001c6d739$42acdad0$8b01a8c0@plab.local> > Content-Type: text/plain; charset="US-ASCII" > > I sure hope not! > > May I second that????? .............I worked freaking hard on my registry! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuttle, > Kimberly (NIH/NCI) [E] > Sent: Tuesday, September 12, 2006 2:23 PM > To: jcarpenter764@aol.com; histonet@pathology.swmed.edu > Subject: RE: [Histonet] Is there any way in the state of New York > > I sure hope not! > > -----Original Message----- > From: jcarpenter764@aol.com [mailto:jcarpenter764@aol.com] > Sent: Tuesday, September 12, 2006 9:12 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Is there any way in the state of New York > > > Is there any way that you can get grandfathered in, in New York without > having to take a exam. After 5 year of experience in the lab I heard > that with your supervisor's approval you can become a certified HT. Can > someone let me know anything about this...Thank you Jennell > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and > security tools, free access to millions of high-quality videos from > across the web, free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 17 > Date: Wed, 13 Sep 2006 10:32:18 -0400 > From: "Margaret Horne" > Subject: RE: [Histonet] Black Walnut stains > To: histonet@lists.utsouthwestern.edu > Message-ID: <4507DE31.25939.206A85@localhost> > Content-Type: text/plain; charset=US-ASCII > > In PEI you can buy PEI Dirt Shirts .; t-shirts stained with PEI red > mud. Great for mothers of kids who play in the mud. :-) > > As for the walnut stains, > Eradasol would work I think , but if not try Avon's Skin So Soft. I > use it to get paint off my hands , brushes and clothes. Doesn't > seem to work on enamel paint but worked fine on an oil base I was > using once. > > good luck , > Margaret > Margaret Horne , > Histology Teaching Assistant, > Dept. of B.SC., > Atlantic Veterinary College, U.P.E.I., > 550 University Ave., Charlottetown, > P.E.I., C1A 4P3 > Canada > > > > > ------------------------------ > > Message: 18 > Date: Wed, 13 Sep 2006 07:18:19 -0700 (PDT) > From: Cheryl > Subject: [Histonet] A question about HT licensure in various states > --which ones require it? > To: histonet@pathology.swmed.edu > Message-ID: <20060913141819.65103.qmail@web50915.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi all- > > Other states I am aware of that require registry for HTs are > Rhode Island > Nevada > Florida > --any others? > > I wrote to the state of NY a few months ago and requested the registry > information. They sent me the information for MT/MLT and CT but nothing for > HT. Could someone enlighten me to the NY regulations in plain language? > > Thanks in advance-- > > Cheryl > > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one Tech at a time. > 281.883.7704 c > 281.852.9457 o > admin@fullstaff.org > > Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade > and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and specialty in > the body of the email. > > ------------------------------ > > Message: 19 > Date: Wed, 13 Sep 2006 07:23:45 -0700 > From: "Robyn Vazquez" > Subject: Re: [Histonet] Surgical Pathology Departments > To: histonet@lists.utsouthwestern.edu, Robert.Lott@TriadHospitals.com > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Robert, > We have an EPIC system that the doctor and nurses input pat's info and we > have electronic medical records now. There is though the staff that types > the letters to the referring doctor, reporting the procedure. They did away > with our transcriptionist... > > Hope this helps, > > Robyn > OHSU > > > > > > ------------------------------ > > Message: 20 > Date: Wed, 13 Sep 2006 11:02:39 -0400 > From: Joyce Friedland > Subject: Re: [Histonet] A question about HT licensure > To: Cheryl > Cc: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; format=flowed > > Hi All, > I am by no means an expert on the subject of New York State Licensure > but one key thing to know is that licensure is in no way connected to > ASCP Registry. They are separate things entirely. > There are a number of pathways to gaining a license in NYS. Some > methods are based on > "grandfathering", some are based on education requirements and some > are based on an exam, which to my knowledge is yet to be written. > Your best way to get all the info is to go to the following web site > and print out everything available as you may need to read through it > several times in order to figure out which is the pathway for your > particular situation : > WWW.OP.NYSED.GOV > Your application needs to be in by 9/30/06 > Hope this helps. > Joyce Friedland > Muhlbauer Derm-Path Lab > Pittsford, NY > 585-586-5166 > > > > > ------------------------------ > > Message: 21 > Date: Wed, 13 Sep 2006 11:39:12 -0500 > From: "Garcia, Amanda" > Subject: [Histonet] Histotech limits > To: > Message-ID: > <8B63039C9DF4554C8FDBF31235F0E14801FC19DA@CPRTEVS02.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Could anyone help me out with a question that was asked today about > there being a limit on number of slides one histotech should be able to > prepare in a regular 8 hour day. That is of course if there is another > person available to handle the constant interruptions that happen > throughout the day. > > Thanks in advance for your help > > > Amanda (Amy) Garcia > > Histology/Pathology > > College Station Medical Center > > (979) 680-5372 office > > (979) 696-5446 fax > > *Mailto:amanda.garcia@triadhospitals.com > > > > This email and any files transmitted with it may contain PRIVILEGED or > > CONFIDENTIAL information and may be read or used only by the intended > > recipient. If you are not the intended recipient of the e-mail or any > > of its attachments, please be advised that you have received this > > e-mail in error and that any use, dissemination, distribution, > > forwarding, printing, or copying of this e-mail or any attached files > > is strictly prohibited. If you have received this e-mail in error, > > please immediately purge it and all attachments and notify the sender > > by reply e-mail or contact the sender at the number listed. > > > > > > > > > ------------------------------ > > Message: 22 > Date: Wed, 13 Sep 2006 11:46:46 -0500 > From: "Garcia, Amanda" > Subject: [Histonet] Automatic coverslippers > To: > Message-ID: > <8B63039C9DF4554C8FDBF31235F0E14801FC19EB@CPRTEVS02.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > I have another question for the experts out there. How much time does > an automatic coverlipper save the histotech? > > Thanks again > > > Amanda (Amy) Garcia > > Histology/Pathology > > College Station Medical Center > > (979) 680-5372 office > > (979) 696-5446 fax > > *Mailto:amanda.garcia@triadhospitals.com > > > > This email and any files transmitted with it may contain PRIVILEGED or > > CONFIDENTIAL information and may be read or used only by the intended > > recipient. If you are not the intended recipient of the e-mail or any > > of its attachments, please be advised that you have received this > > e-mail in error and that any use, dissemination, distribution, > > forwarding, printing, or copying of this e-mail or any attached files > > is strictly prohibited. If you have received this e-mail in error, > > please immediately purge it and all attachments and notify the sender > > by reply e-mail or contact the sender at the number listed. > > > > > > > > > ------------------------------ > > Message: 23 > Date: Wed, 13 Sep 2006 12:51:38 -0400 > From: Carole Fields > Subject: [Histonet] NSH > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi Netters, > Nice time at NSH.... and good turnout. IF anyone took pictures at the > banquet I would really appreciate someone emailing me a few. I took my > camera everywhere and then did not take it to the banquet. Since I do the > newsletter for SC I would like to include a couple of the presentations in > this months issue. > Thank you in advance. > Carole > > Carole Fields, HT,ASCP > Pathology Supervisor > Lexington Medical Center > 2720 Sunset Blvd. > W. Columbia, SC 29169 > > (803) 936-8214 > > > _________________________________ > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed above if one is provided. > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 34, Issue 15 > **************************************** > -------------------------------- Filipa Moraes Mois??s Mallo's Lab Neural Crest Group Instituto Gulbenkian de Ci??ncia Rua da Quinta Grande 6 2800-156, Oeiras Portugal URL: www.igc.gulbenkian.pt fmoraes@igc.gulbenkian.pt Phone: +351 214464525 From BMolinari <@t> heart.thi.tmc.edu Thu Sep 14 05:38:06 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Sep 14 05:38:11 2006 Subject: [Histonet] Automatic coverslippers In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E14801FC19EB@CPRTEVS02.triadhospitals.net> Message-ID: Amy, I am the only tech in this lab. I waited years for a coverslipper and stainer. I finally got both! The coverslipper came first (Sakura Tissue Tek Glas). It was a huge help. Before it arrived I hand coverslipped or, if I had a large amount of slides, I would pack the slides (in staining dishes of xylene_) and take then to the clinical lab to use their automatic coverslipper. So to say my coverslipper is a timesaver is an understatement! Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Amanda Sent: Wednesday, September 13, 2006 11:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic coverslippers I have another question for the experts out there. How much time does an automatic coverlipper save the histotech? Thanks again > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Sep 14 08:55:29 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 14 08:52:17 2006 Subject: [Histonet] CLIA again Message-ID: I am currently looking to hire a grossing tech. I am going through the CLIA regs again about "high complexity testing". Is high complexity testing defined by CLIA anywhere? Also can a person have an AS degree as long as they have the 24 semester hours of sciences? There have been a few that have applied that have taken the "on-line" histology certificate program. Do you think this would count toward the 24 semester hours of sciences? Anyone have a copy of "CLIA For Dummies" laying around? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Nancy.Temple <@t> ssfhs.org Thu Sep 14 09:30:52 2006 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Thu Sep 14 09:31:03 2006 Subject: [Histonet] Microwave Processing Message-ID: Our hospital administration is wanting me to explore the possibility of microwave processing in order to have faster turn around times, mainly on breast core biopsy and GI biopsy cases. Can I please get some feedback from other labs that are doing microwave processing (mainly for just these types of specimens). What brand of processor, changes that had to be made to your workflow, etc. Also, do you process cytology cell blocks in microwave. Any information given will be greatly appreciated. Thank you, Nancy Temple, HT(ASCP) Supervisor Histology/Cytology St. Francis Hospital Indianapolis, IN From mobine <@t> MIT.EDU Thu Sep 14 10:44:23 2006 From: mobine <@t> MIT.EDU (Hector Mobine) Date: Thu Sep 14 10:44:37 2006 Subject: [Histonet] Organ Histology Message-ID: <200609141544.k8EFiOZC000065@outgoing.mit.edu> I am new to histology and was recently assigned the task of developing a protocol for fixing and staining rat adrenal organs, lungs, kidneys following a drug delivery experiment analyzing the development of cardiomyopathy. I have been searching for existing protocols but have been unable to find a detailed protocol for their fixation. I would greatly appreciate it if someone could forward me their protocol for such an experiment or point me in the right direction? Thanks, Hector From Anna.Yates <@t> se.amedd.army.mil Thu Sep 14 11:25:04 2006 From: Anna.Yates <@t> se.amedd.army.mil (Yates, Anna M Ms BACH) Date: Thu Sep 14 11:25:29 2006 Subject: [Histonet] subscribe for a co-worker Message-ID: <17B8554B178BCC42BABB83121934086CFF7F12@amedmlsermc133> timothy.neal@se.amedd.army.mil From cheastys <@t> svm.vetmed.wisc.edu Thu Sep 14 12:56:08 2006 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Thu Sep 14 12:57:39 2006 Subject: [Histonet] Adapter for Lecia Autostainer XL Message-ID: <77d6adbba4ea7049a22023a2c2440bea@svm.vetmed.wisc.edu> Has anyone heard of (or made themselves) an adapter to accommodate the large lantern slides on the XL Autostainer? Thanks, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 From melissa.mazan <@t> tufts.edu Thu Sep 14 13:25:43 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Thu Sep 14 13:25:50 2006 Subject: [Histonet] Re: Histonet Digest, Vol 34, Issue 15 In-Reply-To: <200609131655.k8DGtpks013830@mail-proofpoint-2a.usg.tufts.edu> References: <200609131655.k8DGtpks013830@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <45099EA7.9000001@tufts.edu> We use the tissue from the control animals without BRDU as our control tissue. Melissa Mazan histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Black Walnut stains (Bonner, Janet) > 2. RE: Black Walnut stains (Jackie M O'Connor) > 3. RE: Is there any way in the state of New York > (Tuttle, Kimberly (NIH/NCI) [E]) > 4. Surgical Pathology Departments (Lott, Robert) > 5. BrdU control (Histology Field) > 6. RE: Surgical Pathology Departments (Rittman, Barry R) > 7. RE: BrdU control (Tarango, Mark) > 8. RE: Surgical Pathology Departments (Tarango, Mark) > 9. pH of HIER (donna rossi) > 10. Re: Black Walnut stains (Laurie Reilly) > 11. Re: Surgical Pathology Departments (Joe Nocito) > 12. Re: Black Walnut stains (Joe Nocito) > 13. Re: Adenovirus testing needed (Richard Cartun) > 14. RE: Surgical Pathology Departments (Rene J Buesa) > 15. Re: pH of HIER (Rene J Buesa) > 16. RE: Is there any way in the state of New York (Cheryl Miller) > 17. RE: Black Walnut stains (Margaret Horne) > 18. A question about HT licensure in various states --which ones > require it? (Cheryl) > 19. Re: Surgical Pathology Departments (Robyn Vazquez) > 20. Re: A question about HT licensure (Joyce Friedland) > 21. Histotech limits (Garcia, Amanda) > 22. Automatic coverslippers (Garcia, Amanda) > 23. NSH (Carole Fields) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 12 Sep 2006 14:24:30 -0400 > From: "Bonner, Janet" > Subject: RE: [Histonet] Black Walnut stains > To: "Dasso, Greg (staff)" , "Weems, Joyce" > , "Susan Owens" , "Histonet" > > Message-ID: > <5F31F38C96781A4FBE3196EBC22D478004A5F9@fhosxchmb006.ADVENTISTCORP.NET> > > Content-Type: text/plain; charset=iso-8859-1 > > It would make a great histo lab marker!!! > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg (staff) > Sent: Tue 9/12/2006 12:39 PM > To: Weems, Joyce; Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > > > You just have to wait. It will come off eventually. Walnut husk is a tradional textile dye. > > now, if you could only label something with it. > > /Greg > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce > Sent: Tue 9/12/2006 3:39 AM > To: Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > I don't know of any way, but I would try Eradasol if you have that in > your lab. I didn't have that when I had walnut stained hands growing up! > It will eventually wear off! > > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Owens > Sent: Tuesday, September 12, 2006 3:27 AM > To: Histonet > Subject: [Histonet] Black Walnut stains > > OFF SUBJECT BUT I NEED HELP. > Guys, I need some help... > Yesterday I decided to peel and clean some Black Walnuts to plant later. > Now I've never done this before....My walnut trees came from my > father-in-law's place years ago, where the trees grow wild in the > mountains. When the nuts, which look like hard green apples, fell from > the trees he peeled the thick hard outer cover then cleaned and polish > the inner nut(taste great)...We were there one Christmas and took > several nuts home, planted them, and now have several beautiful big > shade trees. After all these years I decided I needed more trees(lost > several oaks) and I wanted to replace the oaks with the > walnuts...........Sooooooo since they are falling now, a collected > several and started the hard job of peeling.....Under that green outer > skin you fine a light color(yellow-green) meat covering the nut.....I > had peeled a few when I saw that my hands were dirty....Well, it wasn't > dirt.....Seems the clear juice coming from that meat turns brown-dark > brown after a while.....I have tried everything and nothing will touch > it.......Surely someone out there knows what to do!!!! HELP!!!!! > > Thanks > Susan, who should of wore gloves,but who knew. > ohenry@dfw.net > Ohenry Labradors > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Tue, 12 Sep 2006 14:18:40 -0500 > From: "Jackie M O'Connor" > Subject: RE: [Histonet] Black Walnut stains > To: "Bonner, Janet" > Cc: Histonet , "Weems, Joyce" > , histonet-bounces@lists.utsouthwestern.edu, Susan > Owens > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I have a beer dyed shirt from the "Ugly Tuna Saloona", and there are money > dyed shirts, as well as red dirt dyed shirts from Hawaii. > I know this has absolutely nothing to do with anything, but all the cool > people are at the NSH...........(ha). > > > > "Bonner, Janet" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 09/12/2006 01:24 PM > > To > "Dasso, Greg (staff)" , "Weems, Joyce" > , "Susan Owens" , "Histonet" > > cc > > Subject > RE: [Histonet] Black Walnut stains > > > > > > > It would make a great histo lab marker!!! > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dasso, Greg > (staff) > Sent: Tue 9/12/2006 12:39 PM > To: Weems, Joyce; Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > > > You just have to wait. It will come off eventually. Walnut husk is a > tradional textile dye. > > now, if you could only label something with it. > > /Greg > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce > Sent: Tue 9/12/2006 3:39 AM > To: Susan Owens; Histonet > Subject: RE: [Histonet] Black Walnut stains > > I don't know of any way, but I would try Eradasol if you have that in > your lab. I didn't have that when I had walnut stained hands growing up! > It will eventually wear off! > > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Owens > Sent: Tuesday, September 12, 2006 3:27 AM > To: Histonet > Subject: [Histonet] Black Walnut stains > > OFF SUBJECT BUT I NEED HELP. > Guys, I need some help... > Yesterday I decided to peel and clean some Black Walnuts to plant later. > Now I've never done this before....My walnut trees came from my > father-in-law's place years ago, where the trees grow wild in the > mountains. When the nuts, which look like hard green apples, fell from > the trees he peeled the thick hard outer cover then cleaned and polish > the inner nut(taste great)...We were there one Christmas and took > several nuts home, planted them, and now have several beautiful big > shade trees. After all these years I decided I needed more trees(lost > several oaks) and I wanted to replace the oaks with the > walnuts...........Sooooooo since they are falling now, a collected > several and started the hard job of peeling.....Under that green outer > skin you fine a light color(yellow-green) meat covering the nut.....I > had peeled a few when I saw that my hands were dirty....Well, it wasn't > dirt.....Seems the clear juice coming from that meat turns brown-dark > brown after a while.....I have tried everything and nothing will touch > it.......Surely someone out there knows what to do!!!! HELP!!!!! > > Thanks > Susan, who should of wore gloves,but who knew. > ohenry@dfw.net > Ohenry Labradors > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 12 Sep 2006 15:23:05 -0400 > From: "Tuttle, Kimberly \(NIH/NCI\) [E]" > Subject: RE: [Histonet] Is there any way in the state of New York > To: , > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I sure hope not! > > -----Original Message----- > From: jcarpenter764@aol.com [mailto:jcarpenter764@aol.com] > Sent: Tuesday, September 12, 2006 9:12 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Is there any way in the state of New York > > > Is there any way that you can get grandfathered in, in New York without > having to take a exam. After 5 year of experience in the lab I heard > that with your supervisor's approval you can become a certified HT. Can > someone let me know anything about this...Thank you Jennell > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and > security tools, free access to millions of high-quality videos from > across the web, free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Tue, 12 Sep 2006 14:55:47 -0500 > From: "Lott, Robert" > Subject: [Histonet] Surgical Pathology Departments > To: > Message-ID: > <673832E27C45FC4D97EF758FFC777C270149299C@CPRTEVS01.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 12 Sep 2006 12:18:01 -0700 (PDT) > From: Histology Field > Subject: [Histonet] BrdU control > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060912191801.683.qmail@web58613.mail.re3.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi there, > > I was wondering if anyone out there had any BrdU control tissue that they would be willing to share? I am working on a project that requires BrdU staining, but unfortunately, I wasn't given any control tissue. Baboon gut BrdU tissue would be great? Or any other control tissue from any species. Any help would be appreciated! > > Thanks! > N > > > --------------------------------- > Stay in the know. Pulse on the new Yahoo.com. Check it out. > > ------------------------------ > > Message: 6 > Date: Tue, 12 Sep 2006 16:29:19 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] Surgical Pathology Departments > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Tue, 12 Sep 2006 14:43:14 -0700 > From: "Tarango, Mark" > Subject: RE: [Histonet] BrdU control > To: "Histology Field" , > histonet@lists.utsouthwestern.edu > Message-ID: > <5AEC610C1CE02945BD63A395BA763EDE1F98AE@NVCIEXCH02.NVCI.org> > Content-Type: text/plain; charset=us-ascii > > Wouldn't you just use the tissue that you have for the project as the > control? If you injected BrdU into the animal before you sacrificed it, > then it should stain positive (provided the tissue has dividing cells in > it), so I'm thinking that you should be able to use the tissue for the > project as the positive control and tissue from an animal not treated > with BrdU as the negative control. Right? > > > > Mark Adam Tarango HT(ASCP) > > Histology/Immunohistochemistry Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > mtarango@nvcancer.org > > Direct Line (702) 822-5112 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Histology Field > Sent: Tuesday, September 12, 2006 12:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] BrdU control > > Hi there, > > I was wondering if anyone out there had any BrdU control tissue that > they would be willing to share? I am working on a project that requires > BrdU staining, but unfortunately, I wasn't given any control tissue. > Baboon gut BrdU tissue would be great? Or any other control tissue > from any species. Any help would be appreciated! > > Thanks! > N > > > --------------------------------- > Stay in the know. Pulse on the new Yahoo.com. Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "MMS " made the following annotations. > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message > ============================================================================== > > > > > ------------------------------ > > Message: 8 > Date: Tue, 12 Sep 2006 14:56:05 -0700 > From: "Tarango, Mark" > Subject: RE: [Histonet] Surgical Pathology Departments > To: "Rittman, Barry R" , > histonet@lists.utsouthwestern.edu > Message-ID: > <5AEC610C1CE02945BD63A395BA763EDE1F98AF@NVCIEXCH02.NVCI.org> > Content-Type: text/plain; charset=us-ascii > > Dude, I'm totally doing a bunch of work that isn't actually in my job > description. I'm figuring it'll pay off at some point, but so far it > just seems like I have too much to do (including transcribing all the > pathology reports myself). Luckily, our volume for clinical cases is > really low, because doing more than a few reports in a day really takes > up a too much of my time. > > The one good thing about having me do it, is that I catch a lot of > mistakes. Because I work here (as opposed to working for the outside > transcription service we hired in the past), I have access to more > information about the case and I can double check to make sure that > things are correct and accurate. When a report would come back from the > transcription service it seems as if the pathologists wouldn't read more > than the diagnosis. Working on-site with the pathologists, I can just > ask one of them about something when it doesn't look right. > > > > > > Mark Adam Tarango HT(ASCP) > > Histology/Immunohistochemistry Supervisor > > Nevada Cancer Institute > > One Breakthrough Way > > Las Vegas, NV 89135 > > mtarango@nvcancer.org > > Direct Line (702) 822-5112 > > Fax (702) 939-7663 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, > Barry R > Sent: Tuesday, September 12, 2006 2:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Surgical Pathology Departments > > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > "MMS " made the following annotations. > ------------------------------------------------------------------------------ > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message > ============================================================================== > > > > > ------------------------------ > > Message: 9 > Date: Tue, 12 Sep 2006 19:09:14 -0400 > From: "donna rossi" > Subject: [Histonet] pH of HIER > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > Does anyone out there take the pH of their retrieval > solutions ( CITRA, EDTA) after they have been diluted from the > concentrated form? Is this necessary or should we just document the > pH from the company for the concentrated Retrieval ? Thanks, Donna, > SRHS > > > ------------------------------ > > Message: 10 > Date: Wed, 13 Sep 2006 09:07:52 +1000 > From: Laurie Reilly > Subject: Re: [Histonet] Black Walnut stains > To: "Susan Owens" , "Histonet" > > Message-ID: <5.2.0.9.0.20060913090553.00c19348@mail.jcu.edu.au> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Dear All, > Old time timber workers used to use lemon juice and sugar to remove timber > stains, maybe that is worth a try. > > Regards, Laurie. > > > At 02:27 AM 12/09/2006 -0500, Susan Owens wrote: > >>OFF SUBJECT BUT I NEED HELP. >>Guys, I need some help... >>Yesterday I decided to peel and clean some Black Walnuts to plant later. Now >>I've never done this before....My walnut trees came from my father-in-law's >>place years ago, where the trees grow wild in the mountains. When the nuts, >>which look like hard green apples, fell from the trees he peeled the thick >>hard outer cover then cleaned and polish the inner nut(taste great)...We >>were there one Christmas and took several nuts home, planted them, and now >>have several beautiful big shade trees. After all these years I decided I >>needed more trees(lost several oaks) and I wanted to replace the oaks with >>the walnuts...........Sooooooo since they are falling now, a collected >>several and started the hard job of peeling.....Under that green outer skin >>you fine a light color(yellow-green) meat covering the nut.....I had peeled >>a few when I saw that my hands were dirty....Well, it wasn't dirt.....Seems >>the clear juice coming from that meat turns brown-dark brown after a >>while.....I have tried everything and nothing will touch it.......Surely >>someone out there knows what to do!!!! HELP!!!!! >> >>Thanks >>Susan, who should of wore gloves,but who knew. >>ohenry@dfw.net >>Ohenry Labradors >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Mr.Laurie Reilly > School of Veterinary and Biomedical Sciences > James Cook University > Townsville. 4811 > Australia. > > Phone 07 4781 4468 > Fax 07 4779 1526 > > > > > ------------------------------ > > Message: 11 > Date: Tue, 12 Sep 2006 18:52:33 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Surgical Pathology Departments > To: "Rittman, Barry R" , > > Message-ID: <00af01c6d6c6$84a01470$63614542@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Robert, > believe it or not, they tried me out transcribing once. I was a bit > perturbed. I was not having a good day when the transcriptionist called in > sick. My doctor asked me to see if I could transcribe some stat cases. My > response went something like this: "I gross, embed, cut, and stain. Now you > want be to transcribe. Just give me the damn slides and I'll read and sign > out those too" > We now have three transcriptionists and a secretary who can fill in as > needed. Unlike Barry's, our's is not stuffed, bronzed or dusty. She really > is a big help. > Now, Barry, what's this "flaming thing" you talk about? > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > ----- Original Message ----- > From: "Rittman, Barry R" > To: > Sent: Tuesday, September 12, 2006 4:29 PM > Subject: RE: [Histonet] Surgical Pathology Departments > > > Hi Robert > You have just pressed the "not my job" button. > I believe that this is a constant sore point with many histotechs. > As in many labs, tasks that used to be regarded as "secretarial" are now > carried out by the histotechs. in addition to actual histologic tissue > preparation. > While I believe that it is important to understand the various aspects > of a pathology lab I feel that in many cases the extensive training that > histotechs receive in the preparation of tissue is wasted in these > "other tasks". This is not to detract from the skill required to be an > accurate transcriber but I really feel that this is not what most > histotecs. are really paid for. > In our institute there are no "secretaries" but due to political > correctness are known as "administrative assistants". However these are > fewer in number than the original secretarial staff (they also do not > carry out any histological techniques). These extra tasks for the > histotech. are not usually reflected in job descriptions and rarely come > with extra remuneration. > However the good news is that histotechs. are not alone. > Many faculty are typing their own memos and often carrying out numerous > other tasks that were originally carried out by secretaries. > > I stand corrected, we have one secretary here. > This was someone who we perfused and have preserved in the main entrance > so that the image of a real secretary is retained for posterity (has a > nice bronze plaque also). > > Believe that Joe N. would classify this as a potential flaming topic. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Tuesday, September 12, 2006 2:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Surgical Pathology Departments > > Hi Everyone, > > I'm sooooo....sad because I'm not in Phoenix at NSH (first one I've > missed in many years).... but, since I'm not, I have a question for all > of you! > > > > Are their hospital based or even private practice anatomic pathology > laboratories which operate without transcriptionists/secretary/clerical > type individuals for reporting and distribution of reports, etc. and ALL > those things they do? > > > > If so, who performs the duties that would normally be part of their > job.... ?? > > > > Seems I vaguely remember a short thread about this maybe a year ago... > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu From m-degutes <@t> northwestern.edu Thu Sep 14 13:57:55 2006 From: m-degutes <@t> northwestern.edu (Mathew DeGutes) Date: Thu Sep 14 13:58:00 2006 Subject: [Histonet] Alternative to DAPI Message-ID: <20060914185755.D5E9435CE9@casbah.it.northwestern.edu> Are there any alternative nuclear fluorescent stains to DAPI that I can use in the Blue (455-495) spectrum of excitation that will work with frozen sections that are very quickly acetone fixed? I am trying to utilize this for LCM RNA extraction so time is of course a VERY important factor as well. My other fluorophore would be Alexa-555 so I don't want the two to overlap. From j.potas <@t> gmail.com Thu Sep 14 15:05:42 2006 From: j.potas <@t> gmail.com (Jason Potas) Date: Thu Sep 14 15:05:48 2006 Subject: [Histonet] scotts blue recipe?? Message-ID: Hi guys, What a good recipe for Scott's blue to do a H&E stain with Mayers Hematoxylin on spinal cord frozen cut tissue? there is conflicting info between the following 2 sites: http://www.nottingham.ac.uk/pathology/protocols/hande.html and http://stainsfile.info/StainsFile/stain/solutions/scotts-tws.htm which have the same ingredients but opposite quantities!!!! cheers jason -- ______________________________________ Jason Potas (PhD) Laboratorio de Neurobiologia Celular e Molecular Instituto de Biofisica Carlos Chagas Filho - IBCCF Universidade Federal do Rio de Janeiro CCS bloco J sala J1-029 Ilha do Fundao 21941-590 Rio de Janeiro, RJ - Brasil Tel: +5521 2280 4694 +5521 2562 6554 Email: jason@biof.ufrj.br From shive003 <@t> umn.edu Thu Sep 14 15:31:25 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Sep 14 15:31:46 2006 Subject: [Histonet] veterinary IHC Message-ID: <007401c6d83c$c9fac4d0$a1065486@auxs.umn.edu> Hello all, Does anyone know of a laboratory that tests for Porcine Enterovirus through the IHC method? Thanks in advance, Jan Shivers UMN VDL shive003@umn.edu From timothy.macatee <@t> med.nyu.edu Thu Sep 14 16:12:30 2006 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Thu Sep 14 16:15:53 2006 Subject: [Histonet] tissues in cleaning cycle In-Reply-To: Message-ID: Is the Histonet down, no messages today. Or did I quit accidently? Tim On 9/6/06 12:23 PM, "mari.ann.mailhiot@leica-microsystems.com" wrote: > Renee > > As long as the last step on the cleaning cycle is 100% alcohol not water > you can go to xylene and paraffin. But usually cleaning cycles consist of > xylene, 100% alcohol, and water. Water being the last step. > > Also some cleaning cycleces use heat around 60 degrees. Do you want your > specimens subjected to the heat for the amount of time the cleaning cycle > runs? It may be something to think about. > > I don't know what processor you have but you may be able to creat a program > that starts with an alcohol like 95%. You may want to check with your > processor rep on whether you can try and do it. > > Hope this helps. > > Mari Ann Mailhiot BA HT ASCP > Application Specialist > Leica Technical Assistance Center > 800 248 0123 x7267 > 847 236 3063 fax > mari.ann.mailhiot@leica-microsystems.com > www.leica-microsystems.com > > > > "Till, Renee" > du> To > Sent by: histonet@lists.utsouthwestern.edu > histonet-bounces@ cc > lists.utsouthwest > ern.edu Subject > [Histonet] tissues in cleaning > cycle > 09/06/2006 07:49 > AM > > > > > > > > Hello. Any suggestions on what to do with tissues that were accidentally > left in the processor during the cleaning cycle? I would think they need > to be re-infiltrated with paraffin at the very least. I don't know if > our processor will let us do anything but a full processing run. > > > > Renee' Till, HT > > Research Assistant > > Arkansas Children's Nutrition Center > > 1212 Marshall St./N2021 > > Little Rock, AR 72002 > > Office (501)364-2785 > > Fax (501)364-3161 > > > > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From histotecnologia <@t> hotmail.com Thu Sep 14 17:35:50 2006 From: histotecnologia <@t> hotmail.com (Colegio de Histotecnologos) Date: Thu Sep 14 17:35:56 2006 Subject: [Histonet] problems with leica microtome Message-ID: Hello all!! I read about the thik and thin sections problems. I have a 2125 leica microtome. ??ve been having the same problem with mine. I changed the leaf spring, the clamping lever and the clamps, three times, it start working fine and after a few moth it start working bad again. Does any one now if there is a recall for those knife holders?? Its wird that everyone is having the same problem. isn?t? Thanks HT Anaeva Aleo Caracas Venezuela _________________________________________________________________ Consigue aqu? las mejores y mas recientes ofertas de trabajo en Am?rica Latina y USA: [1]Haz clic aqu?... References 1. http://g.msn.com/8HMBES/2743??PS=47575 From c.m.vanderloos <@t> amc.uva.nl Fri Sep 15 01:32:35 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 15 01:32:43 2006 Subject: [Histonet] RE: True Blue chromogen Message-ID: <4622d5462853.4628534622d5@amc.uva.nl> Margaret, TrueBlue yields the type of color that is basically turquoise; one calls it green and others blue. But indeed TrueBlue is more blue than green. The original protocol (also in the book under 'non-commercial visualization systems') using TMB chromogen is more greenish. However, both TMB and TrueBlue are very sensitive/efficient but difficult chromgens in terms of good staining intensity and low background. To my experience it doesn't always work for any antibody. Usually you have to dilute your primary at least 5-50x more than usual. Also dilution of your secondary (2-10x) is optional. Lots of success! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Tue, 12 Sep 2006 11:27:54 -0500 From: "Perry, Margaret" Subject: [Histonet] True Blue chromogen To: I am in need of some clarification on information in Chris van der Loos book "Immunoenzyme Multiple Staining Methods". On page 45 in the chart he lists TMB HRP substrate as producing a green color. In KPL's catalog it lists TMB as blue and I talked to their tech service and they also said it turns blue. Do you have to do something to the TMB to turn it green or does it turn green automatically with IHC ? Are there any other chromogens that turn green? Thanks in advance for your help. Margaret Perry HT(ASCP) South Dakota State University Vet Science From ASelf <@t> gmhsc.com Fri Sep 15 09:53:07 2006 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 15 09:50:44 2006 Subject: [Histonet] test Message-ID: <39836CD6DB61654E8F95A35898C9218602E4EF39@exchange.gmhpost.com> test NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From AGrobe2555 <@t> aol.com Fri Sep 15 10:11:31 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Sep 15 10:11:49 2006 Subject: [Histonet] BRDU Control Message-ID: You could use tissue from your BRDU-treated experimental animal that has a rapid turn-over of cells. I personally have used intestinal tissue. However, this was with PCNA and not with BRDU. Albert Albert C. Grobe, PhD International Heart Institute, Tissue Engineering Lab From naje1972 <@t> yahoo.com Fri Sep 15 10:14:47 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Fri Sep 15 10:14:57 2006 Subject: [Histonet] Not receiving the histonet Message-ID: <20060915151447.53180.qmail@web33003.mail.mud.yahoo.com> Dear Sirs, I have not received any e-mail from the histonet for the last week or so.What is happening? Is your server down? Cynthia Haynes H.T. From anh2006 <@t> med.cornell.edu Fri Sep 15 10:16:52 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Fri Sep 15 10:17:02 2006 Subject: [Histonet] Image analysis software In-Reply-To: <17B8554B178BCC42BABB83121934086CFC037D@amedmlsermc133> References: <17B8554B178BCC42BABB83121934086CFC037D@amedmlsermc133> Message-ID: Dear All, I am looking into acquiring some new image analysis software to upgrade the current situation. Can everyone please recommend to me their favorite platform and some pros and cons of platforms you have used (ImageProPlus, Metamorph etc)? Thanks., Andrea -- From anh2006 <@t> med.cornell.edu Fri Sep 15 10:21:30 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Fri Sep 15 10:21:45 2006 Subject: [Histonet] test Message-ID: I haven't received any Histonet messages since Wednesday, is there something wrong? -- From schnegelsberg <@t> xgene.com Fri Sep 15 10:56:01 2006 From: schnegelsberg <@t> xgene.com (Birthe Schnegelsberg) Date: Fri Sep 15 10:56:16 2006 Subject: [Histonet] don' t receive histonet emails anymore?! In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E14801FC19EB@CPRTEVS02.triadhospitals.net> Message-ID: Hi. Since two days I don' t receive any histonet emails anymore. Can you tell me if my email got lost in the distribution list? Best regards, Birthe Birthe Schnegelsberg Ph.D. Xgene Corporation 2330 Marinship Way, Suite 121 Sausalito, CA 94965 phone 415 339 0516 ext. 104# fax 415 339 0518 www.xgene.com schnegelsberg@xgene.com From Bauer.Karen <@t> mayo.edu Fri Sep 15 11:55:35 2006 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Sep 15 11:55:53 2006 Subject: [Histonet] Test... Message-ID: Just testing... I'm not receiving any emails from histonet. KB ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From immrstambo <@t> hotmail.com Fri Sep 15 18:40:46 2006 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Fri Sep 15 18:40:53 2006 Subject: [Histonet] A question about HT licensure in various states --whichones require it? In-Reply-To: <20060913141819.65103.qmail@web50915.mail.yahoo.com> Message-ID: New York does not require registry with the ASCP-although some hospitals or labs prefer it. September 1st of this year New York is requiring state licensure from New York. The histology license is under the clinical laboratory technologist or clinical laboratory technician license. It is not specific to just histology. The only license that is specific is CYTOLOGY. So in New York State, to practice histology you must have a license for either the 2 yr degree clinical laboratoy technician or 4 year degree clinical laboratory technologist. They are just implementing and many people will be grandfathered, but after this you will need to take an exam to obtain your license. Hope this helps. CHristine Tambasco, HT (ASCP) ST MARY's Hospital @ Amsterdam, New York 12010 ph-518-841-7287 ______________________________________________________________ From: Cheryl To: histonet@pathology.swmed.edu Subject: [Histonet] A question about HT licensure in various states --whichones require it? Date: Wed, 13 Sep 2006 07:18:19 -0700 (PDT) >Hi all- > > Other states I am aware of that require registry for HTs are > Rhode Island > Nevada > Florida > --any others? > > I wrote to the state of NY a few months ago and requested the registry information. They sent me the information for MT/MLT and CT but nothing for HT. Could someone enlighten me to the NY regulations in plain language? > > Thanks in advance-- > > Cheryl > > > > >Cheryl Kerry, HT(ASCP) >Full Staff Inc. >Staffing the AP Lab by helping one Tech at a time. >281.883.7704 c >281.852.9457 o >admin@fullstaff.org > >Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Sep 17 08:54:16 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Sep 17 08:54:23 2006 Subject: [Histonet] may grunwald giemsa Message-ID: <000401c6da60$c477a9d0$eeeea8c0@SERVER01> Hi histonetters, I am looking for a successful procedure for May-Grunwald-Giemsa stain on cytological preps. My colleages in the cyto-lab want to establish it on an automated stainer. They are interested in the right dehydration steps afterwards. Thanks in advance Gudrun Lang From Eric <@t> ategra.com Sat Sep 16 18:52:41 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Mon Sep 18 02:32:39 2006 Subject: [Histonet] Histology Technician - Perm and Temp Jobs in your area Message-ID: Hi - Fellow-Histonetters How are things there in MN/TX/CA/MA/USA ? Below is the updated list of both temp and perm Histology jobs, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech jobs are permanent full-time unless indicated otherwise Permanent Histology Jobs: ------------------------------------------------------ 1. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 2. Washington, D.C - Histotech -perm and temp 3. Northern New Jersey - HistoTech - perm 4. Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech 5. Boston Mass - HistoTech - HistoTech -perm 6. Massachusetts (North of Boston) - perm - Bench Histotech 7. Central Florida -perm- Histotech 8. Southeast Florida - Treasure Coast - perm - HistoTech 9. Southeast Florida - perm - HistoTech 10. Southwest Florida - perm - Bench Histotech/ Supervisor 11. Florida, West Coast - both temp & perm openings- Bench Histotech 12. New Hampshire perm openings - Bench Histotech (opportunity to learn Mohs!) 13. New York ( Syracuse area) - Bench Histotech- perm 14. Central Florida - Bench Histotech- perm 15. New York (Long Island)- Bench Histotech- perm -----------------------------------end perm jobs ---------------------------------------------------- Temporary Assignments ------------------------------- 1. Alaska -Histo Tech Temp - minimum of 16 weeks 2. Washington,DC - HistoTech - minimum of 6 weeks- also Temp to Perm 3. Massachusetts (Boston area) - Histo Tech minimum of 13 weeks (2 people) 4. Pennsylvania - HistoTech- 6 months 5. New Mexico - Histotech- 10-12 weeks 6. Florida (South East)(Treasure Coast)- minimum of 4 weeks possibly longer -------------------------- end list of HistoTech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From sohail_e <@t> yahoo.com Mon Sep 18 04:29:10 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Mon Sep 18 04:31:04 2006 Subject: [Histonet] Protocol for whole mount staining of trachea Message-ID: <20060918092910.70849.qmail@web39504.mail.mud.yahoo.com> Dear All I am currently screening DKK1 transgenic mice for their effect on tracheal vasculature using the whole mount immunoflouresence. I am using Cd31 antibody for that and everything is going well when i do it with retinal whole mount staining but when it comes to trachea, i cant see any blood vessels. What i am thinking is that i am not getting enough penetration. Is there anyone who could kindly guide me a complete protocol for whole mount immunoflouresence of trachea. Thanks Dr.Sohail Ejaz Vascular signalling group Institut f?r Kardiovaskul?re Physiologie Fachbereich Medizin der Johann Wolfgang Goethe-Universit?t, Theodor-Stern-Kai 7 D-60590 Frankfurt am Main Germany --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From rcharles <@t> state.pa.us Mon Sep 18 09:17:41 2006 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Mon Sep 18 09:19:35 2006 Subject: [Histonet] Test Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB570006ACB6E2@enhbgpri04.backup> Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From Rae.Staskiewicz <@t> Illinois.gov Mon Sep 18 10:13:49 2006 From: Rae.Staskiewicz <@t> Illinois.gov (Staskiewicz, Rae) Date: Mon Sep 18 10:31:28 2006 Subject: [Histonet] Test Message-ID: <0909DF46D9192E45A16DC6254AA853D41CE3D8@IL084EX102.Illinois.gov> From jqb7 <@t> cdc.gov Mon Sep 18 10:39:47 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Mon Sep 18 11:59:03 2006 Subject: [Histonet] test: please delete Message-ID: From tkngflght <@t> yahoo.com Mon Sep 18 13:41:57 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Sep 18 14:10:32 2006 Subject: [Histonet] is the histonet broken? Message-ID: <20060918184158.89455.qmail@web50915.mail.yahoo.com> Haven't received emails for a couple of days--please advise? Cheryl From nienhuis <@t> ucla.edu Mon Sep 18 11:19:06 2006 From: nienhuis <@t> ucla.edu (nienhuis@ucla.edu) Date: Mon Sep 18 15:00:19 2006 Subject: [Histonet] Hello? In-Reply-To: References: Message-ID: <20060918091906.3pfv32utc0w8gksk@mail.ucla.edu> Hello, Anybody there? Haven't seen anything from Histonet for a few days. Bob From pmarcum <@t> vet.upenn.edu Mon Sep 18 14:40:32 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Sep 18 15:35:18 2006 Subject: [Histonet] Pennsylvania Histotechnology Society Meeting for October Message-ID: <6.1.1.1.2.20060918152913.019a5828@mail.vet.upenn.edu> Hello Again, We are offering a number of seminars for all phases of Histotechnology and related fields. We have Grossing Surgical Specimens: A Histologist View, FNA for Cytologists and Management seminars the first day with CPT Coding, CAP and Ergonomics. The beginner or someone who is need of a refresher class in IHC will be covered and for the advanced IHC person we will have Multiple Antigen/Chromogen Staining. Just is case you need to negoiate contracts will even have a seminar on that for you. CSI and Entomology will be covered as will some plastics information for research people. HT Readiness and QHIC seminars are available for those who are registering soon. Join us and see what is new, exciting and get those CEUs we all need for the year. The full program is at www.pahisto.org for all. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From soofias2 <@t> yahoo.com Mon Sep 18 15:20:09 2006 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Mon Sep 18 15:46:54 2006 Subject: [Histonet] Slide prep numbers In-Reply-To: <7CAB706201F11843BD26AD516326F0C80166D078@MD1MS007.medimmune.com> Message-ID: <20060918202009.99728.qmail@web39513.mail.mud.yahoo.com> I agree with you. It seems impossible for one person tp cut so many slides in one day. Soofia "Madary, Joseph" wrote: The query was how many slides one can prepare, but that is vague to me, but I am interested in this. By prepare, in my lab preparing is trimming, processing, embedding, microtomy, staining and coverslipping. I honestly do not believe I could trim, process, embed, cut, stain and coverslip 248 per day. I could probably make a fortune in a private histolab with a couple of techs like that. Is it just cutting? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From tissuearray <@t> hotmail.com Mon Sep 18 14:43:08 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Sep 18 15:55:19 2006 Subject: [Histonet] Looking for a fast pnuemocystis stain for frozen sections? Message-ID: I am looking for a procedure for rapid staining of pnuemocystis of frozen sections for our pathologists. I remember having a procedure a long time ago. But I can't find anything like it out there. Has anyone heard of a fungus stain for frozen tissues? Thanks, Thom From immrstambo <@t> hotmail.com Sun Sep 17 13:28:48 2006 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Mon Sep 18 15:57:18 2006 Subject: [Histonet] NYS licensure In-Reply-To: <8C8A48C8016D20E-EEC-2A21@webmail-md11.sysops.aol.com> Message-ID: Yes, if your patholgist will sign to testify that you have been doing histology work for 5 years you can grandfather in for a clinical laboratory technician license. The number of hours that NYS gives is 7,200 clock hours of work prior to september 1, 2006. good luck! christine ______________________________________________________________ From: jcarpenter764@aol.com To: histonet@pathology.swmed.edu Subject: [Histonet] Is there any way in the state of New York Date: Tue, 12 Sep 2006 10:11:39 -0400 > Is there any way that you can get grandfathered in, in New York without having to take a exam. After 5 year of experience in the lab I heard that with your supervisor's approval you can become a certified HT. Can someone let me know anything about this...Thank you Jennell >__________________________________________________________________ ______ >Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Mon Sep 18 14:02:47 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Sep 18 16:03:44 2006 Subject: [Histonet] Looking for Pnuemocysis Staining for Frozen Sections Message-ID: Looking for a rapid staining procedure for fungus for our pathologist to use when doing STAT frozen sections. I remember having one along time ago but I can't find it anywhere. Does any one have a procedure for rapid fungus staining tucked away in their SOPs from years gone by? Thom From cgfields <@t> lexhealth.org Mon Sep 18 08:09:02 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Mon Sep 18 16:09:28 2006 Subject: [Histonet] NSH pictures Message-ID: Hi Again Netters, IF anyone took pictures at the banquet or any of the parties I would really appreciate someone emailing me a few. I took my camera everywhere and then did not take it to the banquet and could not make it to the Thermo party. Since I do the newsletter for SC I would like to include a couple of the presentations in this months issue. Thank you in advance. Carole Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From jfish <@t> gladstone.ucsf.edu Mon Sep 18 15:21:32 2006 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Mon Sep 18 16:21:47 2006 Subject: [Histonet] Where's the histonet? Message-ID: <000001c6db60$081f4c10$8903010a@JFISH> Am I the only one who hasn't had any emails from the histonet list since at least Friday??? Is anyone out there? Or, did I get thrown off the list for some unknown reason? Take care, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 From cjohnston <@t> mdanderson.org Mon Sep 18 13:25:25 2006 From: cjohnston <@t> mdanderson.org (cjohnston@mdanderson.org) Date: Mon Sep 18 16:26:10 2006 Subject: [Histonet] test Message-ID: Carol M. Johnston HT(ASCP) Chief Histology Laboratory Division of Surgery M.D. Anderson Cancer Center 1515 Holcombe Blvd. Unit 017 Houston, Texas 77030 713-745-4625 fax: 713-745-2548 From Tony_Reilly <@t> health.qld.gov.au Mon Sep 18 18:15:25 2006 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Sep 18 18:16:35 2006 Subject: [Histonet] Subscribe Message-ID: Subscribe ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From mtarango <@t> nvcancer.org Mon Sep 18 18:52:27 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Sep 18 18:52:43 2006 Subject: [Histonet] AFB & ALK-1 controls Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F98D9@NVCIEXCH02.NVCI.org> Hi everyone, Does anyone have any AFB or ALK-1 control tissue that they would be willing to share or trade? If so, please e-mail me. Thanks Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From NHeath <@t> Lifespan.org Tue Sep 19 06:36:52 2006 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Tue Sep 19 06:37:08 2006 Subject: [Histonet] please add me Message-ID: <09C945920A6B654199F7A58A1D7D1FDE65EE19@lsexch.lsmaster.lifespan.org> Hi, For some odd reason I have not been receiving my histonet email....so please add me again. Thanks, Nancy nheath@lifespan.org From cpomajzl <@t> cpllabs.com Tue Sep 19 07:15:29 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Tue Sep 19 07:08:57 2006 Subject: [Histonet] Universal Negative Control for IHC??? Message-ID: <001601c6dbe5$4ce026f0$26fca8c0@CSP> My IHC tech had an in-service with our Biocare reps yesterday, and apparently they informed her that there is a universal negative control serum that includes mouse and rabbit IgG. Does anyone know if this is true, and if so, where can this be purchased? Thanks in advance. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. From vbaker60 <@t> yahoo.com Tue Sep 19 08:10:14 2006 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Tue Sep 19 08:10:22 2006 Subject: [Histonet] Is the server down? Message-ID: <20060919131014.92731.qmail@web56811.mail.re3.yahoo.com> Is the list server down __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From lelpers <@t> bioanalytical.com Tue Sep 19 08:17:35 2006 From: lelpers <@t> bioanalytical.com (LaDonna G. Elpers) Date: Tue Sep 19 08:17:58 2006 Subject: [Histonet] paraffin in embedding center Message-ID: <3100BDBD96C0724D9CE9E476BFB57D962DD0FE@basi10003.BIOANALYTICAL.COM> I would like to find out how many people do or do not have molten paraffin in the portion of their embedding center that houses tissue cassettes while embedding. And if you are not keeping cassettes in the molten paraffin your reasoning or theory behind this. Appreciate it! LaDonna LaDonna G. Elpers, BA, HT(ASCP) Manager, Histology BASi (Bioanalytical Systems, Inc.) 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com From rjbuesa <@t> yahoo.com Tue Sep 19 09:24:58 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 19 09:25:08 2006 Subject: [Histonet] Histonet down??? Message-ID: <20060919142458.58021.qmail@web61216.mail.yahoo.com> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From LSebree <@t> uwhealth.org Tue Sep 19 09:46:30 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Sep 19 09:46:43 2006 Subject: [Histonet] Is histonet down? Message-ID: I haven't received any mail from histonet since last Thursday, 914. Is histonet down or is it something within my institution? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From jacobc <@t> mmc.org Tue Sep 19 10:26:55 2006 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Tue Sep 19 10:27:12 2006 Subject: [Histonet] Negative Control for DIF Message-ID: Histonetters! Does anyone have a protocol for DIF on renal biopsies, skin lesions, oral lesions or all of the above that includes a negative control? CAP now requestst that one is done and our lab has never included a negative before in this procedure. Any help would be appreciated. I am also looking for a procedure for doing DIF on paraffin embedded tissue. Any ideas? Chris Jacobs, HT(ASCP), QIHC NorDx Laboratory Scarborough, ME jacobc@mmc.org From JWEEMS <@t> sjha.org Tue Sep 19 11:16:05 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Sep 19 11:16:25 2006 Subject: [Histonet] Methyl Green Pyronin Kits for plasma cell staining Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEAE3@sjhaexc02.sjha.org> Does anyone have a source for a kit for the above stain to be used for plasma cell staining. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kmerriam2003 <@t> yahoo.com Tue Sep 19 11:54:28 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Sep 19 11:54:35 2006 Subject: [Histonet] Tyramide Signal Amplification protocol Message-ID: <20060919165428.74432.qmail@web50301.mail.yahoo.com> Hi, I am looking for the protocol for use of the Perkin-Elmer TSA kit (primarily how to dilute the stuff). The kit we received came with no instructions and we have been unable to contact the vendor. Help! Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Amgen Cambridge, MA --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From JWEEMS <@t> sjha.org Tue Sep 19 12:07:38 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Sep 19 12:07:53 2006 Subject: [Histonet] Immunoperoxidase for plasmacytosis Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEAE7@sjhaexc02.sjha.org> We have a pathologist who is insisting on a stain for plasma cells. Thus the previous question about MGP kits. Second question, do any of you do CD138 for plasma cells. If so, would you please share your source for the antibody and your procedure? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From vanann702 <@t> skmc.gov.ae Tue Sep 19 12:07:57 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Tue Sep 19 12:09:20 2006 Subject: [Histonet] ? blocked email Message-ID: I subscribe to a work-related laboratory list server and have not been receiving any emails from this source histonet@lists.utsouthwestern.edu. Is there a reason for this - has it been blocked by IT? regards Anne From ASelf <@t> gmhsc.com Tue Sep 19 14:41:04 2006 From: ASelf <@t> gmhsc.com (Amy Self) Date: Tue Sep 19 14:38:33 2006 Subject: [Histonet] test Message-ID: <39836CD6DB61654E8F95A35898C9218602E4EF54@exchange.gmhpost.com> test NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From naje1972 <@t> yahoo.com Tue Sep 19 15:43:15 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Tue Sep 19 15:43:22 2006 Subject: [Histonet] I am not receiving the histonet Message-ID: <20060919204315.34927.qmail@web33001.mail.mud.yahoo.com> Dear Sirs I have not received any e-mail from the histonet for the past week or so. Is there a problem with your servers? If so please add my email address to your list again. Thanks in advance Cynthia Haynes H.T. From jmaass <@t> frii.com Tue Sep 19 22:57:30 2006 From: jmaass <@t> frii.com (Janet Maass) Date: Tue Sep 19 22:57:39 2006 Subject: [Histonet] problems receiving mail Message-ID: <000e01c6dc68$e5539f10$0200a8c0@oemcomputer> Hello Something happened and I have not been receiving any histonet information since Sept. 13. Could you please check why I can not receiving mail? Thank you, Janet Maass From Inga.Hansson <@t> neuro.uu.se Wed Sep 20 01:43:11 2006 From: Inga.Hansson <@t> neuro.uu.se (Inga Hansson) Date: Wed Sep 20 01:41:13 2006 Subject: [Histonet] no mail Message-ID: <8cdd1ceef54d9e9e2014514542ec6b1a@neuro.uu.se> Hi, I haven?t recieved any mail from histonet this week...........wondering what?s wrong? Spoke to a swedish collegue and she hasn?t recieved any either! Inga From louise.renton <@t> gmail.com Wed Sep 20 02:14:19 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Sep 20 02:14:23 2006 Subject: [Histonet] histonet Message-ID: what has happened to histonet? I have had no messages for a couple of days now...have i missed something? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From vanann702 <@t> skmc.gov.ae Wed Sep 20 03:06:44 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Wed Sep 20 03:06:20 2006 Subject: [Histonet] CAP Question References: Message-ID: i have had a problem with my connection - am not getting anything from histonet - has anyone else had any problems lately? Annie ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sebree Linda A. Sent: Wed 2006/09/06 05:56 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP Question Laurie, We have a statement in our Laboratory Procedure Manual under "Antibody Optimization" that reads: "The selection of avidin biotin (AB) blocking will be dependent on the specimen source. Examples of tissues benefiting from AB block include kidney and liver. It may be necessary to have more than one protocol programmed to offer the choice of blocking or not." Hope this helps, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, September 05, 2006 4:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone help explain what kind of policy I would need for the following CAP question: ANP.22615: If the laboratory uses an avidin-biotin complex (ABC) detection system (or a related system such as streptavidin-biotin or neutravidin-bioton), is there a policy that addresses nonspecific false positive staining from endogenous bioton? Laurie Colbert Huntington Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Wed Sep 20 10:48:50 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Wed Sep 20 10:49:01 2006 Subject: [Histonet] no messages Message-ID: I haven't received a Histonet message since Friday. Is it just me? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From RFISHER <@t> gbmc.org Wed Sep 20 11:26:49 2006 From: RFISHER <@t> gbmc.org (RENEE FISHER) Date: Wed Sep 20 11:27:14 2006 Subject: [Histonet] question about acid cleaning slides to help adhere tissue to the slide for immuno staining has any Message-ID: <20060920T122649Z_5C8700070000@gbmc.org> question about acid cleaning slides to help adhere tissue to the slide for immuno staining has anyone done this if so is there a procedure? thanks, renee' _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From Dawn.Olszewski <@t> SGMC.ORG Wed Sep 20 12:16:45 2006 From: Dawn.Olszewski <@t> SGMC.ORG (Olszewski, Dawn) Date: Wed Sep 20 12:17:16 2006 Subject: [Histonet] honey as a formalin substitute Message-ID: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043A1@exchange.sgmc.org> Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org From lizhu <@t> acucela.com Wed Sep 20 15:09:59 2006 From: lizhu <@t> acucela.com (Li Zhu) Date: Wed Sep 20 15:11:46 2006 Subject: [Histonet] Good microtomes replacing JB-4 In-Reply-To: <20060830162427.22864.qmail@web61215.mail.yahoo.com> Message-ID: <001601c6dcf0$c03b95b0$7c03a8c0@acucela.local> Dear all histonetters, We're in search for a microtome which could provide similar or higher quality plastic sections as the DuPont Sorvall JB-4 microtome, which has been discontinued. I would appreciate your inputs very much if you could suggest any. Thanks, Li From sohail_e <@t> yahoo.com Wed Sep 20 17:02:03 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Wed Sep 20 17:02:09 2006 Subject: [Histonet] Protcol request for whole mount staining of trachea Message-ID: <20060920220203.43579.qmail@web39507.mail.mud.yahoo.com> Dear All I am currently screening DKK1 transgenic mice for their effect on tracheal vasculature using the whole mount immunoflouresence. I am using Cd31 antibody for that and everything is going well when i do it with retinal whole mount staining but when it comes to trachea, i cant see any blood vessels. What i am thinking is that i am not getting enough penetration. Is there anyone who could kindly guide me a complete protocol for whole mount immunoflouresence of trachea. Thanks Dr.Sohail Ejaz Vascular signalling group Institut f?r Kardiovaskul?re Physiologie Fachbereich Medizin der Johann Wolfgang Goethe-Universit?t, Theodor-Stern-Kai 7 D-60590 Frankfurt am Main Germany --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From histotech <@t> charter.net Wed Sep 20 14:55:41 2006 From: histotech <@t> charter.net (histotech@charter.net) Date: Wed Sep 20 17:02:57 2006 Subject: [Histonet] Re: subscribe Message-ID: <1518023757.1158782141211.JavaMail.root@fepweb12> ---- histotech@charter.net wrote: > -- > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Can someone please tell me why I am not receiving any "histomail" ?!? From RebeNoel <@t> aol.com Wed Sep 20 17:53:02 2006 From: RebeNoel <@t> aol.com (RebeNoel@aol.com) Date: Wed Sep 20 17:53:12 2006 Subject: [Histonet] please unsub my account, thank you Message-ID: From mwahlste <@t> msp.stu.argosyu.edu Wed Sep 20 17:53:47 2006 From: mwahlste <@t> msp.stu.argosyu.edu (Michelle Wahlsten) Date: Wed Sep 20 17:53:55 2006 Subject: [Histonet] Histotechnology student with questions for an assignment Message-ID: <20060920225347.20BED1C6ABB@stu.argosyu.edu> Hello All, I am currently working on an assignment for class regarding histotechnology laboratory procedures and any input would be helpful. If you have the time to answer any or all of these questions, anything would be appreciated!! 1. What accreditation does your laboratory have? Who is responsible for reviewing your lab procedures? How often is this done? 2. About how many histotechnology related procedures exist in your laboratory? How are the organized? Where are they kept?? 3. What is the policy for new employees in relation to learning your lab's procedures? 4. Who is responsible for creating new procedures when necessary? What is the process? 5. How often are procedures updated and how long does this take? Again, thanks for your time and any input you can provide. Michelle-Argosy University-Twin Cities From lizhu <@t> acucela.com Wed Sep 20 19:33:04 2006 From: lizhu <@t> acucela.com (Li Zhu) Date: Wed Sep 20 19:34:26 2006 Subject: [Histonet] FW: Good microtomes replacing JB-4 Message-ID: <000301c6dd15$851c3d70$7c03a8c0@acucela.local> Sending again since it looks like the first time it didn't go through. -----Original Message----- From: Li Zhu [mailto:lizhu@acucela.com] Sent: Wednesday, September 20, 2006 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Good microtomes replacing JB-4 Dear all histonetters, We're in search for a microtome which could provide similar or higher quality plastic sections as the DuPont Sorvall JB-4 microtome, which has been discontinued. I would appreciate your inputs very much if you could suggest any. Thanks, Li From jnocito <@t> satx.rr.com Thu Sep 21 04:33:40 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 21 04:33:48 2006 Subject: [Histonet] yes sir Message-ID: <005401c6dd61$05eb22e0$63614542@yourxhtr8hvc4p> aaaaaaaaaaaaaahhhhhhhhhhhhhhhhhhhhhhhhh. The Histonet is back. I was needing a bad fix. I was shaky, couldn't concentrate, lost my appetite, had bad sweats, couldn't concentrate, bad sweats, couldn't concentrate. Whew. I'm all better now. I missed you all. Joe Nocito BS, PA, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX From MDiCarlo <@t> KaleidaHealth.Org Thu Sep 21 06:51:27 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Sep 21 06:51:34 2006 Subject: [Histonet] Not receiving the histonet In-Reply-To: <20060915151447.53180.qmail@web33003.mail.mud.yahoo.com> Message-ID: <9B4A77DF11463E4FB723D484214AE9BC017EE9B1@KALEXMB02.KaleidaHealth.org> The same goes for me. Peggy DiCarlo HT (ASCP) Orthopaedic Bone Lab Buffalo General Hospital 100 High Street Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cynthia haynes Sent: Friday, September 15, 2006 11:15 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Not receiving the histonet Dear Sirs, I have not received any e-mail from the histonet for the last week or so.What is happening? Is your server down? Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Laurie.Cote <@t> cytyc.com Thu Sep 21 06:54:09 2006 From: Laurie.Cote <@t> cytyc.com (Cote, Laurie) Date: Thu Sep 21 06:54:04 2006 Subject: [Histonet] wax temperatures Message-ID: <3E76917CB874EF4DB6DF79DD5CEBC11712145C06@nahqmails9.cytyc.com> Does anyone know what happens to Paraplast Xtra if it is 10 degrees above its melting point? From sbreeden <@t> nmda.nmsu.edu Thu Sep 21 07:06:44 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Sep 21 07:06:48 2006 Subject: [Histonet] NEW MEXICO SOCIETY FOR HISTOLOGY Message-ID: Just a reminder that the New Mexico Society for Histology will hold its first Annual Meeting in over three years this coming October 21st in Albuquerque. For information on the meeting and/or registration forms, contact me via email or phone. "Come on down!" P.S. This Friday will be the resumption of the Friday Hour of Fuming. Get your ammo ready! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From portera <@t> msu.edu Thu Sep 21 08:09:10 2006 From: portera <@t> msu.edu (Amy Porter) Date: Thu Sep 21 08:08:16 2006 Subject: [Histonet] Yeh glad to be back & ?? Message-ID: <002401c6dd7f$20828e90$8e7a0923@HistoJJ> Is anyone out there doing Tunel on FFPE acid decalcifed sections. I am trying to stain for apoptotic cells using a Tunel kit on decalcified mouse femurs. Not having much success, if anyone has any tips or do's / don'ts I would really appreciate the information. Thanks in advance, Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu From chiggerson <@t> memhosp.com Thu Sep 21 08:36:43 2006 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Thu Sep 21 08:36:56 2006 Subject: [Histonet] Re: Histonet Plasmacytosis In-Reply-To: <20060920212519.19112.qmail@mail.memhosp.com> Message-ID: Joyce, We use CD138 pre-dilute from Cell Marque on the Ventana Benchmark XT. They have several different sizes of pre-dilute and concentrate. Our pathologists love it. We use hidradenitis suppurativa as a positive control (it has a lot more plasma cells than tonsil and is easier to get than plasmacytoma). Procedure: Mild cell conditioning (Tris/EDTA buffer, pH 8) for 30 minutes. AB incubation 32 min @ 37 degrees. with Amplification Hope this helps. Cindy Message: 1 Date: Tue, 19 Sep 2006 13:07:38 -0400 From: "Weems, Joyce" Subject: [Histonet] Immunoperoxidase for plasmacytosis To: "Histonet \(E-mail\)" Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEAE7@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" We have a pathologist who is insisting on a stain for plasma cells. Thus the previous question about MGP kits. Second question, do any of you do CD138 for plasma cells. If so, would you please share your source for the antibody and your procedure? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax From Malcolm.McCallum <@t> tamut.edu Thu Sep 21 08:53:21 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Thu Sep 21 08:54:11 2006 Subject: [Histonet] wax temperatures References: <3E76917CB874EF4DB6DF79DD5CEBC11712145C06@nahqmails9.cytyc.com> Message-ID: I don't know about 10 degrees above melting, but if held too warm for too long it will cause the carbon chains to break down. VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cote, Laurie Sent: Thu 9/21/2006 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wax temperatures Does anyone know what happens to Paraplast Xtra if it is 10 degrees above its melting point? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Thu Sep 21 08:55:41 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Thu Sep 21 08:55:51 2006 Subject: [Histonet] NSH Phoenix Message-ID: Hi again Netters, If anyone took pictures at the NSH banquet would you please email me just a couple. I left my camera in my room (smart) and I need just one or two for the SC newsletter. It would be greatly appreciated... You can send them to my hospital email. Thank you in advance. cgfields@lexhealth.org. Carole Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 (803) 936-8214 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From vazquezr <@t> ohsu.edu Thu Sep 21 09:01:18 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Sep 21 09:01:51 2006 Subject: [Histonet] don' t receive histonet emails anymore?! Message-ID: I haven't rec'd any either. Aprox 2 days. Robyn From Rcartun <@t> harthosp.org Thu Sep 21 09:12:39 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Sep 21 09:13:20 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043A1@exchange.sgmc.org> References: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043A1@exchange.sgmc.org> Message-ID: <451265970200007700002015@hcnwgwds01.hh.chs> The article is in the most recent issue (September 2006) of The Journal of Histotechnology. The authors also presented a poster on this topic at the NSH meeting Phoenix last week. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Olszewski, Dawn" 09/20/06 1:16 PM >>> Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org From cbass <@t> bidmc.harvard.edu Thu Sep 21 09:19:23 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Thu Sep 21 09:20:07 2006 Subject: [Histonet] mounting fluorescent tissue Message-ID: <1F8AFAA9-AF22-4F92-802F-866B1B6AA50E@bidmc.harvard.edu> Hi, I have what might be a silly question. I am dealing with a lot of native fluorescence, particularly in the brain, 40 um floating sections. At some point I want to mount the sections to observe the native fluorescence. I have been doing this in a dimly lit room, but it can be difficult at times. Do you think there would be a loss of signal if I mount the sections under normal light conditions? Also, if I choose to do immunofluorescence should I be careful, I heard that FITC staining should be done in the dark and I assume the mounting should as well. Any advice is appreciated. Thanks, Caroline From jqb7 <@t> cdc.gov Thu Sep 21 09:21:43 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Thu Sep 21 09:31:02 2006 Subject: [Histonet] don' t receive histonet emails anymore?! Message-ID: I have.... Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Thursday, September 21, 2006 10:01 AM To: histonet@lists.utsouthwestern.edu; schnegelsberg@xgene.com Subject: Re: [Histonet] don' t receive histonet emails anymore?! I haven't rec'd any either. Aprox 2 days. Robyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Don.Birgerson <@t> leica-microsystems.com Thu Sep 21 09:38:35 2006 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Thu Sep 21 09:39:41 2006 Subject: [Histonet] FW: Good microtomes replacing JB-4 In-Reply-To: <000301c6dd15$851c3d70$7c03a8c0@acucela.local> Message-ID: Hi Li, Our Leica-Microsystems has a line of microtomes RM2255 and RM2265 that can handle your requirements and improve on your sample options. These microtomes come from a refined routine microtome and can cut 0.25 to 60 microns and section sizes from 2mm to 25mm. There are six different knife holders and specimen holders so it can be configured for the task the operators is trying to accomplish. Give me a call and I can elaborate for you. Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 7:00 to 4:00 pm CDT "Li Zhu" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] FW: Good microtomes replacing JB-4 09/20/2006 07:33 PM Sending again since it looks like the first time it didn't go through. -----Original Message----- From: Li Zhu [mailto:lizhu@acucela.com] Sent: Wednesday, September 20, 2006 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Good microtomes replacing JB-4 Dear all histonetters, We're in search for a microtome which could provide similar or higher quality plastic sections as the DuPont Sorvall JB-4 microtome, which has been discontinued. I would appreciate your inputs very much if you could suggest any. Thanks, Li _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mauger <@t> email.chop.edu Thu Sep 21 09:50:53 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Thu Sep 21 09:51:44 2006 Subject: [Histonet] BK virus Message-ID: Hi, Does anyone know a source for BK virus + control slides, or cell cultures?? Thanks.Positive patient tissue is always scant! Jo Mauger From LBUSTAMANTE <@t> cvm.tamu.edu Thu Sep 21 10:16:06 2006 From: LBUSTAMANTE <@t> cvm.tamu.edu (Lin Bustamante) Date: Thu Sep 21 10:16:40 2006 Subject: [Histonet] Technovit 9100 Kit Message-ID: <45126666020000B900002657@CVM.TAMU.EDU> Please help me to find the distributor of this kit in the USA. I tried many companies with no luck. Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Histology Lab Dept. of Veterinary Anatomy and Public Health Texas A&M University College Station, TX 77843-4458 From liz <@t> premierlab.com Thu Sep 21 10:44:58 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Sep 21 10:40:37 2006 Subject: [Histonet] Yeh glad to be back & ?? In-Reply-To: <002401c6dd7f$20828e90$8e7a0923@HistoJJ> Message-ID: <002201c6dd94$e49f47e0$0300a8c0@domain.Premier> Amy I did this back a few years ago and found that the process of formic acid decalcification caused all the nuclei to be positive. From what I remember the use of formic acid is not compatible with the tunel technique. Why don't you try using cleaved caspase 3 immunohistochemistry instead. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, September 21, 2006 7:09 AM To: Histonet Subject: [Histonet] Yeh glad to be back & ?? Is anyone out there doing Tunel on FFPE acid decalcifed sections. I am trying to stain for apoptotic cells using a Tunel kit on decalcified mouse femurs. Not having much success, if anyone has any tips or do's / don'ts I would really appreciate the information. Thanks in advance, Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1766 (20060921) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From gu.lang <@t> gmx.at Thu Sep 21 10:42:18 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 21 10:42:20 2006 Subject: AW: [Histonet] Technovit 9100 Kit In-Reply-To: <45126666020000B900002657@CVM.TAMU.EDU> Message-ID: <000001c6dd94$855de7a0$eeeea8c0@SERVER01> I think EMS sells this kit. http://www.emsdiasum.com/microscopy/search/results.asp?Prod=technovit&Submit 2=Search Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lin Bustamante Gesendet: Donnerstag, 21. September 2006 17:16 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Technovit 9100 Kit Please help me to find the distributor of this kit in the USA. I tried many companies with no luck. Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Histology Lab Dept. of Veterinary Anatomy and Public Health Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Sep 21 10:48:45 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Sep 21 10:44:21 2006 Subject: [Histonet] Image analysis software In-Reply-To: Message-ID: <002301c6dd95$6bd4eda0$0300a8c0@domain.Premier> Andrea We use image pro plus and also automeasure from zeiss. Both work well, but our image analysis guy prefers the image pro plus because he can write his own code for the image pro and we can't do that with the automeasure software we have currently we would need to buy an additional software package from zeiss called commander. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Friday, September 15, 2006 9:17 AM To: Histonet Subject: [Histonet] Image analysis software Dear All, I am looking into acquiring some new image analysis software to upgrade the current situation. Can everyone please recommend to me their favorite platform and some pros and cons of platforms you have used (ImageProPlus, Metamorph etc)? Thanks., Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1766 (20060921) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From rhrogers <@t> iupui.edu Thu Sep 21 10:39:26 2006 From: rhrogers <@t> iupui.edu (Rogers, Rhonda) Date: Thu Sep 21 10:47:41 2006 Subject: [Histonet] Myc tag Antibody Message-ID: <0263AA7A7CB6FF42AA4048567A26EBC9458A0A@iu-mssg-mbx107.ads.iu.edu> Does anyone know a good Myc tag antibody (clone 9E10)? I'll be using it on formalin-fixed paraffin embedded sections of mouse tissue. Would like to avoid frozen sections, if possible. Any advice would be appreciated. Thanks, Rhonda From rjbuesa <@t> yahoo.com Thu Sep 21 10:58:51 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 10:59:04 2006 Subject: [Histonet] may grunwald giemsa In-Reply-To: <000401c6da60$c477a9d0$eeeea8c0@SERVER01> Message-ID: <20060921155851.22275.qmail@web61221.mail.yahoo.com> Reduce the dehydration steps to a minimum, starting with 95% EthOL and make them as fast as possible. Clearing will pose no problems for decolorization, but dehydration will. Ren? J. Gudrun Lang wrote: Hi histonetters, I am looking for a successful procedure for May-Grunwald-Giemsa stain on cytological preps. My colleages in the cyto-lab want to establish it on an automated stainer. They are interested in the right dehydration steps afterwards. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Thu Sep 21 11:00:20 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 11:00:27 2006 Subject: [Histonet] Universal Negative Control for IHC??? In-Reply-To: <001601c6dbe5$4ce026f0$26fca8c0@CSP> Message-ID: <20060921160020.59361.qmail@web61224.mail.yahoo.com> True, from DAKO-Cytomation. Ren? J. Chris Pomajzl wrote: My IHC tech had an in-service with our Biocare reps yesterday, and apparently they informed her that there is a universal negative control serum that includes mouse and rabbit IgG. Does anyone know if this is true, and if so, where can this be purchased? Thanks in advance. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From pmcardle <@t> ebsciences.com Thu Sep 21 11:02:21 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Thu Sep 21 11:02:33 2006 Subject: [Histonet] Technovit 9100 Kit In-Reply-To: <45126666020000B900002657@CVM.TAMU.EDU> References: <45126666020000B900002657@CVM.TAMU.EDU> Message-ID: <4512B78D.2060907@ebsciences.com> Hi: Energy Beam Sciences, East Granby, Connecticut (www.ebsciences.com), is the US distributor of Heraeus-Kulzer Technovit 7100, 8100 and 9100 kits and components. PLease don't hesitate to contact me with any questions or issues you may have. Best regards, Phil -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" You must be the change you want to see in the world. - Mahatma Gandhi Lin Bustamante wrote: > Please help me to find the distributor of this kit in the USA. > I tried many companies with no luck. > Thank you. Lin. > > Lin S. Bustamante, B.Sc.; HT(ASCP) > Histology Lab > Dept. of Veterinary Anatomy and Public Health > Texas A&M University > College Station, TX 77843-4458 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Sep 21 11:14:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 11:14:44 2006 Subject: [Histonet] paraffin in embedding center In-Reply-To: <3100BDBD96C0724D9CE9E476BFB57D962DD0FE@basi10003.BIOANALYTICAL.COM> Message-ID: <20060921161438.87662.qmail@web61223.mail.yahoo.com> Any reason for not keeping the cassettes in the molten paraffin is a wrong reason. Cassttes with tissues out of the retort should not be kept out of melted paraffin, there will always be some solidification around the tissue that will impede a really continuous paraffin medium when the block is casted in the mold. This will affect sectioning, in some cases more than in others, but it is a risk not worth taking. The side of the embedding center designed to receive the cassettes should be filled with molten paraffin. Just my opinion (not shared by some, by the way!). Ren? J., "LaDonna G. Elpers" wrote: I would like to find out how many people do or do not have molten paraffin in the portion of their embedding center that houses tissue cassettes while embedding. And if you are not keeping cassettes in the molten paraffin your reasoning or theory behind this. Appreciate it! LaDonna LaDonna G. Elpers, BA, HT(ASCP) Manager, Histology BASi (Bioanalytical Systems, Inc.) 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From rjbuesa <@t> yahoo.com Thu Sep 21 11:29:55 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 11:30:02 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043A1@exchange.sgmc.org> Message-ID: <20060921162955.30514.qmail@web61213.mail.yahoo.com> Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From rjbuesa <@t> yahoo.com Thu Sep 21 11:35:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 11:35:35 2006 Subject: Fwd: Re: [Histonet] Histonet down??? Message-ID: <20060921163529.68713.qmail@web61220.mail.yahoo.com> Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From rjbuesa <@t> yahoo.com Thu Sep 21 11:46:47 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 11:46:53 2006 Subject: Fwd: Re: [Histonet] honey as a formalin substitute Message-ID: <20060921164647.42194.qmail@web61217.mail.yahoo.com> Rene J Buesa wrote: Date: Thu, 21 Sep 2006 09:29:55 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] honey as a formalin substitute To: "Olszewski, Dawn" , histonet@lists.utsouthwestern.edu Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From rjbuesa <@t> yahoo.com Thu Sep 21 11:47:23 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 11:47:29 2006 Subject: Fwd: Re: [Histonet] Histonet down??? Message-ID: <20060921164724.33591.qmail@web61212.mail.yahoo.com> Rene J Buesa wrote: Date: Thu, 21 Sep 2006 09:35:28 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] Histonet down??? To: histonet@lists.utsouthwestern.edu Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com --------------------------------- Get your email and more, right on the new Yahoo.com From Barry.R.Rittman <@t> uth.tmc.edu Thu Sep 21 11:48:00 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Sep 21 11:48:06 2006 Subject: [Histonet] honey as a formalin substitute Message-ID: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Steven2146 <@t> aol.com Thu Sep 21 11:49:05 2006 From: Steven2146 <@t> aol.com (Steven2146@aol.com) Date: Thu Sep 21 11:49:24 2006 Subject: [Histonet] Re: Histonet Digest, Vol 34, Issue 24 Message-ID: Hi... I believe I saw a reference to that in the latest issue of NSH magazine. Steven Lee From TJJ <@t> Stowers-Institute.org Thu Sep 21 11:52:38 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Sep 21 11:52:52 2006 Subject: [Histonet] Re: Tunel staining with acid decalcified samples Message-ID: (formerly "Yeh glad to be back & ??") Amy, I'm not surprised you are having difficulty with TUNEL staining on acid decalcified samples. You are denaturing the DNA with your decalcifier. You might look into using EDTA instead, and with mouse femurs it shouldn't take more than a week for adequate decalcification. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From JGarfield <@t> lifecell.com Thu Sep 21 11:54:13 2006 From: JGarfield <@t> lifecell.com (Jacqueline D. Garfield) Date: Thu Sep 21 11:55:11 2006 Subject: [Histonet] Technovit 9100 Kit Message-ID: Lin, You can find this product at Electron Microscopy Sciences (EMS). Their address and phone number: 321 Morris Road Box 251 Fort Washington, PA 19034 (215) 646-1566 (800) 523-5874 www.emsdiasum.com Regards, Jackie Garfield -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, September 21, 2006 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 Kit Please help me to find the distributor of this kit in the USA. I tried many companies with no luck. Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Histology Lab Dept. of Veterinary Anatomy and Public Health Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu Sep 21 11:56:09 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Sep 21 12:07:18 2006 Subject: [Histonet] wax temperatures References: <3E76917CB874EF4DB6DF79DD5CEBC11712145C06@nahqmails9.cytyc.com> Message-ID: <003401c6dd9e$d68f61d0$130e4246@yourlk4rlmsu> Based on my experience with similar waxes, but not Paraplast Xtra itself, not much. It gets a little less viscous and likely infiltrates fractionally faster, but for usual periods of time involved in processing it has no other effect. If kept for too long (weeks or months) the colour changes as a result of chemical alteration from the heat. I used to keep some wax of this kind in an oven set at about 70C for remelting blocks and such, and I experienced no difficulties with it, although it is not a recommended practice. Bryan Llewellyn From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cote, Laurie Sent: Thu 9/21/2006 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wax temperatures Does anyone know what happens to Paraplast Xtra if it is 10 degrees above its melting point? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 21 12:20:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 21 12:20:31 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: Message-ID: <20060921172025.7835.qmail@web61223.mail.yahoo.com> Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From Kari.Zajic <@t> HCAhealthcare.com Thu Sep 21 12:56:42 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Sep 21 12:56:56 2006 Subject: [Histonet] Histonet down??? In-Reply-To: <20060921163529.68713.qmail@web61220.mail.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC69F@ORLEV03.hca.corpad.net> Rene` for Histonet president!! :) he always has all the answers!!! yay! thanks Rene`! :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital email: Kari.Zajic@HCAHealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Histonet down??? Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbryant <@t> labpath.com Thu Sep 21 13:47:42 2006 From: sbryant <@t> labpath.com (Susan Bryant) Date: Thu Sep 21 13:54:32 2006 Subject: [Histonet] universal negative control for IHC Message-ID: <00dd01c6ddae$8181e720$8d01a8c0@labpath.com> I buy mine from Biocare. It is as they say, both mouse and rabbit IgG. The mouse IgG contains a spectrum of IgG1, IgG2, IgG3, IgG4 and IgM subclasses. Susan Bryant Knoxville, TN From gcallis <@t> montana.edu Thu Sep 21 15:50:23 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 21 15:50:27 2006 Subject: [Histonet] Student writing summary on honey as a fixative and other comments Message-ID: <6.0.0.22.1.20060921142032.01b49708@gemini.msu.montana.edu> Dawn, Have your student contact the author of this publication for further discussion on this topic. I also suggest your student access some of the references cited in the publication - it would be a good exercise for doing a reference i.e. literature search to broaden their knowledge base. Philip.Bryant@bromor-tr.wales.nhs.uk Although honey may not be an ideal formalin substitute and the honey was from two species of bees found in Oman, I was intrigued by the topic both how it was used in the paper and historically as a preservative and dehydrant in other parts of the world. I found the publication unique and rather interesting that something like this can be done, maybe without the better results of formalin fixation. I also saw the poster at 2006 NSH S/C, where many were just as curious as I was honey as a formalin substitute, it was a popular poster to visit. If people are NOT happy with the publication as it is, a letter to the JOH editor would be in order and allow the authors to make further comments on what points may have been not addressed as they may not be looking in on Histonet to see current commentary or critique of their work. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ttruscot <@t> vetmed.wsu.edu Thu Sep 21 17:06:39 2006 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Sep 21 17:06:44 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: <20060921162955.30514.qmail@web61213.mail.yahoo.com> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44C06CE09@cvm36.vetmed.wsu.edu> I would also be curious to know if honey collected from plants high in estrogens would affect any type of estrogen staining after processing tissue fixed in honey. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 8:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shirley.jen <@t> roche.com Thu Sep 21 19:54:45 2006 From: shirley.jen <@t> roche.com (Jen, Shirley) Date: Thu Sep 21 19:54:57 2006 Subject: [Histonet] Job Opportunity In-Reply-To: <200609211709.EFD72947@mailgate4.roche.com> Message-ID: <6A39BF27EAB27743887E0425BD51196B0499AB16@rplmsem1.nala.roche.com> We have a full time histology position available at Roche Palo Alto in California. The position requires working with a variety of laboratory animal tissues for pre-clincal drug safety studies, performing wet tissue trimming to staining for routine paraffin processing, special stains, frozen sections, IHC and other specialized techniques. Interested person please send resume to shirley.jen@roche.com. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, September 21, 2006 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 34, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fwd: Re: [Histonet] Histonet down??? (Rene J Buesa) 2. Fwd: Re: [Histonet] honey as a formalin substitute (Rene J Buesa) 3. Fwd: Re: [Histonet] Histonet down??? (Rene J Buesa) 4. RE: honey as a formalin substitute (Rittman, Barry R) 5. Re: Histonet Digest, Vol 34, Issue 24 (Steven2146@aol.com) 6. Re: Tunel staining with acid decalcified samples (Johnson, Teri) 7. RE: Technovit 9100 Kit (Jacqueline D. Garfield) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 Sep 2006 09:35:28 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] Histonet down??? To: histonet@lists.utsouthwestern.edu Message-ID: <20060921163529.68713.qmail@web61220.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com ------------------------------ Message: 2 Date: Thu, 21 Sep 2006 09:46:47 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] honey as a formalin substitute To: histonet@lists.utsouthwestern.edu Message-ID: <20060921164647.42194.qmail@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Rene J Buesa wrote: Date: Thu, 21 Sep 2006 09:29:55 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] honey as a formalin substitute To: "Olszewski, Dawn" , histonet@lists.utsouthwestern.edu Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. ------------------------------ Message: 3 Date: Thu, 21 Sep 2006 09:47:23 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] Histonet down??? To: histonet@lists.utsouthwestern.edu Message-ID: <20060921164724.33591.qmail@web61212.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Rene J Buesa wrote: Date: Thu, 21 Sep 2006 09:35:28 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] Histonet down??? To: histonet@lists.utsouthwestern.edu Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com --------------------------------- Get your email and more, right on the new Yahoo.com ------------------------------ Message: 4 Date: Thu, 21 Sep 2006 11:48:00 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] honey as a formalin substitute To: "Rene J Buesa" , "Olszewski, Dawn" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 21 Sep 2006 12:49:05 EDT From: Steven2146@aol.com Subject: [Histonet] Re: Histonet Digest, Vol 34, Issue 24 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi... I believe I saw a reference to that in the latest issue of NSH magazine. Steven Lee ------------------------------ Message: 6 Date: Thu, 21 Sep 2006 11:52:38 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Tunel staining with acid decalcified samples To: Message-ID: Content-Type: text/plain; charset="us-ascii" (formerly "Yeh glad to be back & ??") Amy, I'm not surprised you are having difficulty with TUNEL staining on acid decalcified samples. You are denaturing the DNA with your decalcifier. You might look into using EDTA instead, and with mouse femurs it shouldn't take more than a week for adequate decalcification. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ------------------------------ Message: 7 Date: Thu, 21 Sep 2006 12:54:13 -0400 From: "Jacqueline D. Garfield" Subject: RE: [Histonet] Technovit 9100 Kit To: "Lin Bustamante" , Message-ID: Content-Type: text/plain; charset="us-ascii" Lin, You can find this product at Electron Microscopy Sciences (EMS). Their address and phone number: 321 Morris Road Box 251 Fort Washington, PA 19034 (215) 646-1566 (800) 523-5874 www.emsdiasum.com Regards, Jackie Garfield -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, September 21, 2006 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 Kit Please help me to find the distributor of this kit in the USA. I tried many companies with no luck. Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Histology Lab Dept. of Veterinary Anatomy and Public Health Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 34, Issue 25 **************************************** From Linresearch <@t> aol.com Thu Sep 21 20:09:27 2006 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Thu Sep 21 20:09:35 2006 Subject: [Histonet] Fink-Heimer Silver Stain Message-ID: <374.a1a69e7.324491c7@aol.com> Hello, Does anyone have a protocol for Fink-Heimer Silver Stain for nerve cells and endings that they would be willing to share with me? Thanks, Lin From micro <@t> superlink.net Thu Sep 21 21:31:42 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Thu Sep 21 21:31:54 2006 Subject: [Histonet] mounting fluorescent tissue References: <1F8AFAA9-AF22-4F92-802F-866B1B6AA50E@bidmc.harvard.edu> Message-ID: <027301c6ddef$3db1e9f0$55893cd1@DJ4VDH31> a little light won't do any harm! Just think of the high intensity of UV light you will bombard your samples under the microscope !!!! Regards, Markus F. Meyenhofer ----- Original Message ----- From: "Caroline Bass" To: "histonet" Sent: Thursday, September 21, 2006 10:19 AM Subject: [Histonet] mounting fluorescent tissue > Hi, I have what might be a silly question. I am dealing with a lot of > native fluorescence, particularly in the brain, 40 um floating sections. > At some point I want to mount the sections to observe the native > fluorescence. I have been doing this in a dimly lit room, but it can be > difficult at times. Do you think there would be a loss of signal if I > mount the sections under normal light conditions? Also, if I choose to > do immunofluorescence should I be careful, I heard that FITC staining > should be done in the dark and I assume the mounting should as well. > > Any advice is appreciated. Thanks, Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ree3 <@t> leicester.ac.uk Fri Sep 22 03:27:32 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 22 03:27:46 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: <20060921172025.7835.qmail@web61223.mail.yahoo.com> Message-ID: Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Fri Sep 22 04:47:34 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 22 04:34:48 2006 Subject: [Histonet] FW: Good microtomes replacing JB-4 Message-ID: Dear Li, We produce the Bright 5040 microtome that will more than suit your requirements as its specifications and options will give you a far greater range of applications than the discontinued DuPont Sorvall JB-4 microtome. If you could please let me know your location I will have a distributor from your region contact you. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 ------Original Message----- From: Li Zhu [mailto:lizhu@acucela.com] Sent: Wednesday, September 20, 2006 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Good microtomes replacing JB-4 Dear all histonetters, We're in search for a microtome which could provide similar or higher quality plastic sections as the DuPont Sorvall JB-4 microtome, which has been discontinued. I would appreciate your inputs very much if you could suggest any. Thanks, Li From Stephen.Eyres <@t> sanofi-aventis.com Fri Sep 22 04:46:54 2006 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Fri Sep 22 04:47:57 2006 Subject: [Histonet] honey as a formalin substitute Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D05F356@ALPW31.f2.enterprise> Oooo, there was a sting in the tail there!!! Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 22, 2006 9:28 AM To: Rene J Buesa; Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stephen.Eyres <@t> sanofi-aventis.com Fri Sep 22 04:53:59 2006 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Fri Sep 22 04:54:13 2006 Subject: [Histonet] honey as a formalin substitute Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D05F357@ALPW31.f2.enterprise> On the other hand you could ask then to pollen the other one, or your views were nectar to my ears, or then again, this paper seems to be causing a flap!!! I'll stop now, I need a lay down. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 22, 2006 9:28 AM To: Rene J Buesa; Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Sep 22 05:16:09 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Fri Sep 22 05:18:21 2006 Subject: [Histonet] honey as a formalin substitute Message-ID: Hey, you catch more flies with honey......... Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen.Eyres@sanofi-aventis.com Sent: Friday, September 22, 2006 5:47 AM To: ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Oooo, there was a sting in the tail there!!! Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 22, 2006 9:28 AM To: Rene J Buesa; Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stephen.Eyres <@t> sanofi-aventis.com Fri Sep 22 05:22:59 2006 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Fri Sep 22 05:26:00 2006 Subject: [Histonet] honey as a formalin substitute Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D05F359@ALPW31.f2.enterprise> Wasp that you say? I don't know swat you mean. Or am I just bumbling about! I have wings to do so Legsleave this now as I am six of it!! Steve -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCID) [mailto:jqb7@cdc.gov] Sent: Friday, September 22, 2006 11:16 AM To: Eyres, Stephen SMA/GB; ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Hey, you catch more flies with honey......... Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen.Eyres@sanofi-aventis.com Sent: Friday, September 22, 2006 5:47 AM To: ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Oooo, there was a sting in the tail there!!! Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 22, 2006 9:28 AM To: Rene J Buesa; Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Sep 22 06:11:03 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 22 06:11:30 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: <90B6684A9D6DAF468F7A5DC148754E1D05F359@ALPW31.f2.enterprise> Message-ID: This thread seems to be droning on a bit!!. -----Original Message----- From: Stephen.Eyres@sanofi-aventis.com [mailto:Stephen.Eyres@sanofi-aventis.com] Sent: 22 September 2006 11:23 To: jqb7@cdc.gov; ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Wasp that you say? I don't know swat you mean. Or am I just bumbling about! I have wings to do so Legsleave this now as I am six of it!! Steve -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCID) [mailto:jqb7@cdc.gov] Sent: Friday, September 22, 2006 11:16 AM To: Eyres, Stephen SMA/GB; ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Hey, you catch more flies with honey......... Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen.Eyres@sanofi-aventis.com Sent: Friday, September 22, 2006 5:47 AM To: ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Oooo, there was a sting in the tail there!!! Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 22, 2006 9:28 AM To: Rene J Buesa; Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Fri Sep 22 06:43:13 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Fri Sep 22 06:43:21 2006 Subject: [Histonet] FW: Good microtomes replacing JB-4 References: <000301c6dd15$851c3d70$7c03a8c0@acucela.local> Message-ID: <029f01c6de3c$49a79000$55893cd1@DJ4VDH31> JB-4 Microtomes are still available! We have several reconditioned JB-4 Microtomes available, guaranteed, with various knife holder / block holder configurations. Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 EM and Histology Services, Lab Equipment since 1977 ----- Original Message ----- From: "Li Zhu" To: Sent: Wednesday, September 20, 2006 8:33 PM Subject: [Histonet] FW: Good microtomes replacing JB-4 > Sending again since it looks like the first time it didn't go through. > > -----Original Message----- > From: Li Zhu [mailto:lizhu@acucela.com] > Sent: Wednesday, September 20, 2006 1:10 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Good microtomes replacing JB-4 > > Dear all histonetters, > > We're in search for a microtome which could provide similar or higher > quality plastic sections as the DuPont Sorvall JB-4 microtome, which has > been discontinued. I would appreciate your inputs very much if you could > suggest any. > > Thanks, > Li > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mpetey <@t> aol.com Fri Sep 22 08:41:02 2006 From: mpetey <@t> aol.com (mpetey@aol.com) Date: Fri Sep 22 08:41:09 2006 Subject: [Histonet] lab procedures Message-ID: <8C8AC63E168B95D-E10-3082@WEBMAIL-RA07.sysops.aol.com> I am a histotechnology student, and I have a few questions for an assignment. Thanks for your time! What accreditation does your lab have? Who reviews your lab procedures and how often is it done? How many histotechnology related procedures does your lab have? How are they organized and where are they kept? What is the policy for new employees learning your lab procedures? Who creates new procedures when needed and what is the process? Are your lab procedures always followed? When and how do you vary from them, and is this a norm in your lab? Thank you! ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From ryaskovich <@t> dir.nidcr.nih.gov Fri Sep 22 08:41:31 2006 From: ryaskovich <@t> dir.nidcr.nih.gov (Ruth Yaskovich) Date: Fri Sep 22 08:42:46 2006 Subject: [Histonet] Student writing summary on honey as a fixative and other comments In-Reply-To: <6.0.0.22.1.20060921142032.01b49708@gemini.msu.montana.edu> Message-ID: Gayle, I agree this paper is interesting it has us all talking about it. I have my own bees and so does my neighbor and I can get plenty of spare tissues so I think I'm going to repeat the experiment. I didn't have much honey this year but I'll use next years and compare with local honey and some purchased from the grocery store. I'll try some on perfused rats and try some C.N.S. Tissues this should be fun. It will be an interesting topic at the Maryland Beekeepers Meeting. I'll let everyone know what happens. Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Pain and Neurobiology Branch On 9/21/06 4:50 PM, "Gayle Callis" wrote: > Dawn, > > Have your student contact the author of this publication for further > discussion on this topic. I also suggest your student access some of the > references cited in the publication - it would be a good exercise for doing > a reference i.e. literature search to broaden their knowledge base. > > Philip.Bryant@bromor-tr.wales.nhs.uk > > Although honey may not be an ideal formalin substitute and the honey was > from two species of bees found in Oman, I was intrigued by the topic both > how it was used in the paper and historically as a preservative and > dehydrant in other parts of the world. I found the publication unique > and rather interesting that something like this can be done, maybe without > the better results of formalin fixation. I also saw the poster at 2006 NSH > S/C, where many were just as curious as I was honey as a formalin > substitute, it was a popular poster to visit. > > If people are NOT happy with the publication as it is, a letter to the JOH > editor would be in order and allow the authors to make further comments on > what points may have been not addressed as they may not be looking in on > Histonet to see current commentary or critique of their work. > > > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpetey <@t> aol.com Fri Sep 22 08:45:38 2006 From: mpetey <@t> aol.com (mpetey@aol.com) Date: Fri Sep 22 08:45:55 2006 Subject: [Histonet] transmissible spongiform encephalopathies Message-ID: <8C8AC6485A83985-E10-30D5@WEBMAIL-RA07.sysops.aol.com> I am a histotechnology student, and I am doing a research project on bovine spongiform encephalopathy (mad cow disease). I was wondering if anyone has had any cases of any of the transmissible spongiform encephalopathies? What part of the brain was submitted for exam? What fixative did you use? What stains did you perform? Was the case negative or positive? Thank you for your time! ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From scott142 <@t> comcast.net Fri Sep 22 08:48:24 2006 From: scott142 <@t> comcast.net (scott142@comcast.net) Date: Fri Sep 22 08:48:31 2006 Subject: [Histonet] Alcian Yellow **HELP** Message-ID: <092220061348.18307.4513E9A800083D3B000047832200734748CDCBCE9B9B010C9C@comcast.net> I routinely run a procedure that works very well with old lots of Alcian Yellow. Unfortunately I have no more lots of old Alcian Yellow. If anyone has inventory of Alcian Yellow that they do not use, I would be very eager to purchase it from you. Thanks you... From sohail_e <@t> yahoo.com Fri Sep 22 09:00:20 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Fri Sep 22 09:00:26 2006 Subject: [Histonet] Tracheal wholmount staining Message-ID: <20060922140020.88162.qmail@web39504.mail.mud.yahoo.com> Dear All I am currently screening DKK1 transgenic mice for their effect on tracheal vasculature using the whole mount immunoflouresence. I am using Cd31 antibody for that and everything is going well when i do it with retinal whole mount staining but when it comes to trachea, i cant see any blood vessels. What i am thinking is that i am not getting enough penetration. Is there anyone who could kindly guide me a complete protocol for whole mount immunoflouresence of trachea. Thanks Dr.Sohail Ejaz histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: wax temperatures (Bryan Llewellyn) 2. RE: honey as a formalin substitute (Rene J Buesa) 3. RE: Re: [Histonet] Histonet down??? (Zajic Kari) 4. universal negative control for IHC (Susan Bryant) 5. Student writing summary on honey as a fixative and other comments (Gayle Callis) 6. RE: honey as a formalin substitute (Truscott, Tom) 7. Job Opportunity (Jen, Shirley) 8. Fink-Heimer Silver Stain (Linresearch@aol.com) 9. Re: mounting fluorescent tissue (Markus F. Meyenhofer) 10. RE: honey as a formalin substitute (Edwards, R.E.) 11. RE: FW: Good microtomes replacing JB-4 (Alan Bright) 12. RE: honey as a formalin substitute (Stephen.Eyres@sanofi-aventis.com) 13. RE: honey as a formalin substitute (Stephen.Eyres@sanofi-aventis.com) 14. RE: honey as a formalin substitute (Bartlett, Jeanine (CDC/CCID/NCID)) 15. RE: honey as a formalin substitute (Stephen.Eyres@sanofi-aventis.com) 16. RE: honey as a formalin substitute (Edwards, R.E.) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 Sep 2006 09:56:09 -0700 From: Bryan Llewellyn Subject: Re: [Histonet] wax temperatures To: Histonet Message-ID: <003401c6dd9e$d68f61d0$130e4246@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original Based on my experience with similar waxes, but not Paraplast Xtra itself, not much. It gets a little less viscous and likely infiltrates fractionally faster, but for usual periods of time involved in processing it has no other effect. If kept for too long (weeks or months) the colour changes as a result of chemical alteration from the heat. I used to keep some wax of this kind in an oven set at about 70C for remelting blocks and such, and I experienced no difficulties with it, although it is not a recommended practice. Bryan Llewellyn From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cote, Laurie Sent: Thu 9/21/2006 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wax temperatures Does anyone know what happens to Paraplast Xtra if it is 10 degrees above its melting point? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 21 Sep 2006 10:20:25 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] honey as a formalin substitute To: "Rittman, Barry R" , "Olszewski, Dawn" , histonet@lists.utsouthwestern.edu Message-ID: <20060921172025.7835.qmail@web61223.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. ------------------------------ Message: 3 Date: Thu, 21 Sep 2006 13:56:42 -0400 From: "Zajic Kari" Subject: RE: Re: [Histonet] Histonet down??? To: "Rene J Buesa" , Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC69F@ORLEV03.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Rene` for Histonet president!! :) he always has all the answers!!! yay! thanks Rene`! :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital email: Kari.Zajic@HCAHealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Histonet down??? Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 21 Sep 2006 14:47:42 -0400 From: "Susan Bryant" Subject: [Histonet] universal negative control for IHC To: Message-ID: <00dd01c6ddae$8181e720$8d01a8c0@labpath.com> Content-Type: text/plain; charset="iso-8859-1" I buy mine from Biocare. It is as they say, both mouse and rabbit IgG. The mouse IgG contains a spectrum of IgG1, IgG2, IgG3, IgG4 and IgM subclasses. Susan Bryant Knoxville, TN ------------------------------ Message: 5 Date: Thu, 21 Sep 2006 14:50:23 -0600 From: Gayle Callis Subject: [Histonet] Student writing summary on honey as a fixative and other comments To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060921142032.01b49708@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Dawn, Have your student contact the author of this publication for further discussion on this topic. I also suggest your student access some of the references cited in the publication - it would be a good exercise for doing a reference i.e. literature search to broaden their knowledge base. Philip.Bryant@bromor-tr.wales.nhs.uk Although honey may not be an ideal formalin substitute and the honey was from two species of bees found in Oman, I was intrigued by the topic both how it was used in the paper and historically as a preservative and dehydrant in other parts of the world. I found the publication unique and rather interesting that something like this can be done, maybe without the better results of formalin fixation. I also saw the poster at 2006 NSH S/C, where many were just as curious as I was honey as a formalin substitute, it was a popular poster to visit. If people are NOT happy with the publication as it is, a letter to the JOH editor would be in order and allow the authors to make further comments on what points may have been not addressed as they may not be looking in on Histonet to see current commentary or critique of their work. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 6 Date: Thu, 21 Sep 2006 15:06:39 -0700 From: "Truscott, Tom" Subject: RE: [Histonet] honey as a formalin substitute To: "Rene J Buesa" , "Olszewski, Dawn" , Message-ID: <35CF12B690D8CA4E95375A36B4E7B44C06CE09@cvm36.vetmed.wsu.edu> Content-Type: text/plain; charset="iso-8859-1" I would also be curious to know if honey collected from plants high in estrogens would affect any type of estrogen staining after processing tissue fixed in honey. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 8:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 21 Sep 2006 17:54:45 -0700 From: "Jen, Shirley" Subject: [Histonet] Job Opportunity To: Message-ID: <6A39BF27EAB27743887E0425BD51196B0499AB16@rplmsem1.nala.roche.com> Content-Type: text/plain; charset="iso-8859-1" We have a full time histology position available at Roche Palo Alto in California. The position requires working with a variety of laboratory animal tissues for pre-clincal drug safety studies, performing wet tissue trimming to staining for routine paraffin processing, special stains, frozen sections, IHC and other specialized techniques. Interested person please send resume to shirley.jen@roche.com. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, September 21, 2006 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 34, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fwd: Re: [Histonet] Histonet down??? (Rene J Buesa) 2. Fwd: Re: [Histonet] honey as a formalin substitute (Rene J Buesa) 3. Fwd: Re: [Histonet] Histonet down??? (Rene J Buesa) 4. RE: honey as a formalin substitute (Rittman, Barry R) 5. Re: Histonet Digest, Vol 34, Issue 24 (Steven2146@aol.com) 6. Re: Tunel staining with acid decalcified samples (Johnson, Teri) 7. RE: Technovit 9100 Kit (Jacqueline D. Garfield) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 Sep 2006 09:35:28 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] Histonet down??? To: histonet@lists.utsouthwestern.edu Message-ID: <20060921163529.68713.qmail@web61220.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Fellow Histonetters: I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet". Regards Ren? J. LINDA MARGRAF wrote: Date: Thu, 21 Sep 2006 08:47:42 -0500 From: "LINDA MARGRAF" To: Subject: Re: [Histonet] Histonet down??? It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator >>> Rene J Buesa 09/19/06 9:24 AM >>> Histonet server: Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise Ren? J. Buesa --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com ------------------------------ Message: 2 Date: Thu, 21 Sep 2006 09:46:47 -0700 (PDT) From: Rene J Buesa Subject: Fwd: Re: [Histonet] honey as a formalin substitute To: histonet@lists.utsouthwestern.edu Message-ID: <20060921164647.42194.qmail@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Rene J Buesa wrote: Date: Thu, 21 Sep 2006 09:29:55 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] honey as a formalin substitute To: "Olszewski, Dawn" , histonet@lists.utsouthwestern.edu Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. === message truncated === __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From lblazek <@t> digestivespecialists.com Fri Sep 22 09:12:24 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Sep 22 09:12:47 2006 Subject: [Histonet] Alcian Yellow **HELP** Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684AAA@bruexchange.digestivespecialists.com> I'd also be interested! Want to share? Or if anyone knows of a good substitute that info would be appreciated too. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of scott142@comcast.net Sent: Friday, September 22, 2006 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Yellow **HELP** I routinely run a procedure that works very well with old lots of Alcian Yellow. Unfortunately I have no more lots of old Alcian Yellow. If anyone has inventory of Alcian Yellow that they do not use, I would be very eager to purchase it from you. Thanks you... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DOOLEEO <@t> shands.ufl.edu Fri Sep 22 09:14:39 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Fri Sep 22 09:15:02 2006 Subject: [Histonet] Thomsen-Friedenreich Antigen Message-ID: Dear Histonetters and Vendors, Does anyone know of a vendor that supplies an antibody which works on paraffin embedded sections which stains for Thomsen-Friedenreich Antigen? ( t antigen) We used to get it from DAKO and they said they did not carry it anymore. Thanks in advance Elaine Dooley HTL Shands Teaching Hospital 352-265-0111 ext 72117 From sbreeden <@t> nmda.nmsu.edu Fri Sep 22 09:17:31 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Sep 22 09:17:41 2006 Subject: [Histonet] Friday Hour of Fuming Message-ID: Determined as I am to reinstitute the FHoF, I have been in too good a mood all week to be annoyed. Alas, I was devoid of a Subject de Fume until I came to work and found that the network administrator had been doing whatever-network-administrators-do and my computer was locked!! I had to track him down (at 6:00 a.m., mind you) and promise him my firstborn - but here I am. So, someone out there gets to pick the Fume du Jour! If no one steps up to the plate, I'll just whine about dripping plastic gallon jugs again... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From mauger <@t> email.chop.edu Fri Sep 22 09:31:39 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 22 09:32:24 2006 Subject: [Histonet] flame Dako Message-ID: Lets all flame DAKO! What's the problem? They are discontinuing so many products, and we have been waiting for months to receive our orders!! I was trying to be patient, but I have just about had it!! I have switched to many LabVision products. Get with it, DAKO, or you will lose customers! That feels better, Jo Mauger From gcallis <@t> montana.edu Fri Sep 22 09:37:38 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 22 09:37:51 2006 Subject: [Histonet] honey as a formalin substitute In-Reply-To: <35CF12B690D8CA4E95375A36B4E7B44C06CE09@cvm36.vetmed.wsu.ed u> References: <20060921162955.30514.qmail@web61213.mail.yahoo.com> <35CF12B690D8CA4E95375A36B4E7B44C06CE09@cvm36.vetmed.wsu.edu> Message-ID: <6.0.0.22.1.20060922083525.01b6b238@gemini.msu.montana.edu> Tom, Contact the author , Philip.Bryant@bromor-tr.wales.nhs.uk. I am sure he would be able to answer your question. At 04:06 PM 9/21/2006, you wrote: >I would also be curious to know if honey collected from plants high in >estrogens would affect any type of estrogen staining after processing >tissue fixed in honey. Tom Truscott Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From sjchtascp <@t> yahoo.com Fri Sep 22 09:55:11 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Sep 22 09:55:19 2006 Subject: [Histonet] Seeking Employment Message-ID: <20060922145511.97467.qmail@web38201.mail.mud.yahoo.com> Good morning "all" from bee-buzzing Southern Wisconsin. My position at a research lab in Madison was elininated 2 weeks ago . I'm looking to somthing in either southern Wisconsin or northern Illinois. Doesn't need to be full time, part time, weekends or as needed would be fine. I'm not quite ready to be put out to pasture yet.. Thanks everyone, Steve --------------------------------- Get your email and more, right on the new Yahoo.com From schraz13 <@t> yahoo.com Fri Sep 22 10:12:54 2006 From: schraz13 <@t> yahoo.com (Scheherazade Humphrey) Date: Fri Sep 22 10:12:58 2006 Subject: [Histonet] Histotechnology Laboratory Procedure Assignment Message-ID: <20060922151254.34250.qmail@web81008.mail.mud.yahoo.com> Hello Everyone, My name is Scheherazade Humphrey and I am currently a student at Argosy University in the Twin Cities. I was asked by my instructor to post a listserve to get possible answers and help on one of my assignments. If any one is able to answer any of the following questions, that would be greatly appreciated. Thank you. The following questions are : 1. What accreditation does your laboratory have? Who is responsible for reviewing your lab procedures? How often is it done? 2. About how many histotechnology related procedures exist in your laboratory? How are they organized? Where are they kept? 3. What is the policy for new employees in relation to learning the laboratory procedures? 4. Who is responsible for creating new procedures when needed? What is the process? Thank you for participating in my Questionnaire for my Histotechnology Laboratory Procedure Assignment. Scheherazade H. --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From soofias2 <@t> yahoo.com Fri Sep 22 10:17:15 2006 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Fri Sep 22 10:17:20 2006 Subject: [Histonet] Seeking Employment In-Reply-To: <20060922145511.97467.qmail@web38201.mail.mud.yahoo.com> Message-ID: <20060922151715.22662.qmail@web39505.mail.mud.yahoo.com> Hi Steve, Did you check my last email?I gave you my supervisor's name and phone number she was looking for two employees. She is a manager of several labs. I believe she hired one histonet and she is looking for another one. Did you talk to her already? Soofia Steven Coakley wrote: Good morning "all" from bee-buzzing Southern Wisconsin. My position at a research lab in Madison was elininated 2 weeks ago . I'm looking to somthing in either southern Wisconsin or northern Illinois. Doesn't need to be full time, part time, weekends or as needed would be fine. I'm not quite ready to be put out to pasture yet.. Thanks everyone, Steve --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From thoward <@t> unm.edu Fri Sep 22 10:47:21 2006 From: thoward <@t> unm.edu (Tamara A Howard) Date: Fri Sep 22 10:47:26 2006 Subject: [Histonet] The infamous honey paper Message-ID: Would someone be willing/able to send me a pdf of this paper? Our library does not have a subscription and I'd like to see what all the fuss (I'll avoid saying "buzz") is about. Thanks! *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From tkngflght <@t> yahoo.com Fri Sep 22 12:01:57 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Sep 22 12:02:07 2006 Subject: [Histonet] Jobs--several openings In-Reply-To: <20060922145511.97467.qmail@web38201.mail.mud.yahoo.com> Message-ID: <20060922170157.60255.qmail@web50915.mail.yahoo.com> Hi All- It was SO QUIET last week! I MISSED you guys :) I have a number of open permanent positions from entry level through management and IHC all around the states. I don't push people into the jobs on the list but it'd be nice to help some of these labs--they are all great places to be a tech--I've benched in some myself. If you're seriously looking, we'll work FOR you to find the right job. Give me a call or email and I'll go over the list and maybe I can help you AND the lab?!! Everyone wins! Also, I am always seeking techs with great skills and flexible dispositions for temp assignments. As a temp you'd work as an employee of my company (I'm a working Histotech--it helps) and we keep you working. Take a look at the article in the NSH edition of Advance (Aug 28, p. 18) and you can check with folks already on staff--let them tell you if I'm any good at this :) One week to three months--we're busy and can always use good help. I welcome all callers--new grads, registered, unregistered or OJT. My favorite part of this is meeting smart people who love what they do--good histology. Please do not respond to the histonet--send your inquiries (or flames--it is Friday) to: admin@fullstaff.org or call 800.756.3309. I answer til 10PM CST, or leave a message and I'll call you back . Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From chewy71874 <@t> aol.com Fri Sep 22 12:27:32 2006 From: chewy71874 <@t> aol.com (chewy71874@aol.com) Date: Fri Sep 22 12:27:56 2006 Subject: [Histonet] Prefer fixative Message-ID: <8C8AC8385BABA6B-F74-448B@mblk-r30.sysops.aol.com> How many out there are using Prefer fixative? What are your results with IHC? Ellen Yee ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Erin.Wrona <@t> kp.org Fri Sep 22 12:30:05 2006 From: Erin.Wrona <@t> kp.org (Erin.Wrona@kp.org) Date: Fri Sep 22 12:31:39 2006 Subject: [Histonet] San Francisco job opening Message-ID: Hi all, SF Kaiser's Regional Immuno lab has an tech opening. The hours would be 3-1:30 PM. Immuno experience and HT certification preferred, but not required. The salary is among the best (possibly the best) in the SF bay area, and benefits are excellent. If you're interested, check out Kaiser's web site at www.kp.org and search on histology technician as the job title. Erin NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From gu.lang <@t> gmx.at Fri Sep 22 12:45:18 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 22 12:45:20 2006 Subject: [Histonet] proteolytic antigenretrieval Message-ID: <000601c6de6e$dead5c70$eeeea8c0@SERVER01> Hi, what type of enzyme do you recommend for proteolytik antigenretrieval? (Sigma Number? or similar) thank you Gudrun Lang Histolab Akh Linz Austria From tpmorken <@t> labvision.com Fri Sep 22 13:04:50 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Fri Sep 22 13:05:04 2006 Subject: [Histonet] t writing summary on honey as a fixative and other comments Message-ID: Honey for use for anything is a popular subject in the Arab peninsula (one of the authors of this paper is of apparent Arab descent). In Arabia honey is considered a food, a preservative for food and medical substance used for treatment of various ailments. Some consider it a practically magical elixer. When I worked in Saudi Arabia our med tech / histotech students constantly wanted to do studies using honey for one thing or another so when I saw this topic I instantly knew the impetus for it. This current article may actually be informative if it is considered in the context of preserving tissue for other uses, not necessarily for high quality histology work. I know that is not the authors intention, but research in one field often informs another field without intending to. I do agree that the reviewers should have been much more critical of the authors contention that the histology of honey- vs formalin-fixed tissue is comparable (describe to what degree it is comparable). It is obvious the pictures were chosen to prove their point. For instance, the picture of a formalin-fixed rat kidney glomerulus is the worst I have ever seen (tissue is badly distorted) while the honey-fixed tissue is reasonable for gross structure. Even so, it does show that honey does preserve tissue to a cetain degree which may be useful for something. The fact a certain honey was used is probably not too critical for this study, but honey from different areas do have different chemicals in them depending on the flowers the nectar came from. Tim Morken Gayle, I agree this paper is interesting it has us all talking about it. I have my own bees and so does my neighbor and I can get plenty of spare tissues so I think I'm going to repeat the experiment. I didn't have much honey this year but I'll use next years and compare with local honey and some purchased from the grocery store. I'll try some on perfused rats and try some C.N.S. Tissues this should be fun. It will be an interesting topic at the Maryland Beekeepers Meeting. I'll let everyone know what happens. Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Pain and Neurobiology Branch On 9/21/06 4:50 PM, "Gayle Callis" wrote: > Dawn, > > Have your student contact the author of this publication for further > discussion on this topic. I also suggest your student access some of the > references cited in the publication - it would be a good exercise for doing > a reference i.e. literature search to broaden their knowledge base. > > Philip.Bryant@bromor-tr.wales.nhs.uk > > Although honey may not be an ideal formalin substitute and the honey was > from two species of bees found in Oman, I was intrigued by the topic both > how it was used in the paper and historically as a preservative and > dehydrant in other parts of the world. I found the publication unique > and rather interesting that something like this can be done, maybe without > the better results of formalin fixation. I also saw the poster at 2006 NSH > S/C, where many were just as curious as I was honey as a formalin > substitute, it was a popular poster to visit. > > If people are NOT happy with the publication as it is, a letter to the JOH > editor would be in order and allow the authors to make further comments on > what points may have been not addressed as they may not be looking in on > Histonet to see current commentary or critique of their work. > > > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) Tim Morken Product Development Lab Vision - Neomarkers 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 email: tpmorken@labvision.com web: www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm From shive003 <@t> umn.edu Fri Sep 22 13:29:04 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 22 14:31:42 2006 Subject: [Histonet] proteolytic antigenretrieval References: <000601c6de6e$dead5c70$eeeea8c0@SERVER01> Message-ID: <01c401c6de7d$b58505f0$a1065486@auxs.umn.edu> Proteinase K (Dako; S3004). Jan Shivers IHC Lab UMN VetDiagLab USA ----- Original Message ----- From: "Gudrun Lang" To: "Histonetliste (Histonetliste)" Sent: Friday, September 22, 2006 12:45 PM Subject: [Histonet] proteolytic antigenretrieval > Hi, > what type of enzyme do you recommend for proteolytik antigenretrieval? > (Sigma Number? or similar) > > thank you > Gudrun Lang > > Histolab > Akh Linz > Austria > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Fri Sep 22 13:33:45 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 22 14:31:43 2006 Subject: [Histonet] off-topic re: our Tim Morken Message-ID: <01c501c6de7d$b58e7bd0$a1065486@auxs.umn.edu> I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers From pruegg <@t> ihctech.net Fri Sep 22 14:43:43 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 22 14:43:58 2006 Subject: [Histonet] Yeh glad to be back & ?? In-Reply-To: <002201c6dd94$e49f47e0$0300a8c0@domain.Premier> Message-ID: <003901c6de7f$69dcd1d0$6601a8c0@Patsy> Amy, I agree with Liz on this, it is hard enough with Tunnel to distinguish between necrosis and apoptosis without adding decal. I much prefer caspase, you can count on the positive cells being just those under going apoptosis. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, September 21, 2006 9:45 AM To: 'Amy Porter'; 'Histonet' Subject: RE: [Histonet] Yeh glad to be back & ?? Amy I did this back a few years ago and found that the process of formic acid decalcification caused all the nuclei to be positive. From what I remember the use of formic acid is not compatible with the tunel technique. Why don't you try using cleaved caspase 3 immunohistochemistry instead. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, September 21, 2006 7:09 AM To: Histonet Subject: [Histonet] Yeh glad to be back & ?? Is anyone out there doing Tunel on FFPE acid decalcifed sections. I am trying to stain for apoptotic cells using a Tunel kit on decalcified mouse femurs. Not having much success, if anyone has any tips or do's / don'ts I would really appreciate the information. Thanks in advance, Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1766 (20060921) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Fri Sep 22 15:07:18 2006 From: portera <@t> msu.edu (Amy Porter) Date: Fri Sep 22 15:06:18 2006 Subject: [Histonet] Yeh glad to be back & ?? References: <003901c6de7f$69dcd1d0$6601a8c0@Patsy> Message-ID: <002901c6de82$b48e0980$8e7a0923@HistoJJ> Thanks Patsy - Are you using Caspase 3 ? or another Caspase for that and is it active or cleaved - just curious. I think I am going to have trouble all the way around. We are trying to do immuno on mouse tibias that have been acid decalcified. After several postings I am trying to look at alternative decalcification methods, I have been able to obtain some forelimbs from mice just to work with the decaling and processing. Any pointers that you would have would be greatly appreciated. Amy ----- Original Message ----- From: "Patsy Ruegg" To: "'Liz Chlipala'" ; "'Amy Porter'" ; "'Histonet'" Sent: Friday, September 22, 2006 3:43 PM Subject: RE: [Histonet] Yeh glad to be back & ?? > Amy, > I agree with Liz on this, it is hard enough with Tunnel to distinguish > between necrosis and apoptosis without adding decal. I much prefer > caspase, > you can count on the positive cells being just those under going > apoptosis. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz > Chlipala > Sent: Thursday, September 21, 2006 9:45 AM > To: 'Amy Porter'; 'Histonet' > Subject: RE: [Histonet] Yeh glad to be back & ?? > > Amy > > I did this back a few years ago and found that the process of formic acid > decalcification caused all the nuclei to be positive. From what I > remember > the use of formic acid is not compatible with the tunel technique. Why > don't you try using cleaved caspase 3 immunohistochemistry instead. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter > Sent: Thursday, September 21, 2006 7:09 AM > To: Histonet > Subject: [Histonet] Yeh glad to be back & ?? > > Is anyone out there doing Tunel on FFPE acid decalcifed sections. I am > trying to stain for apoptotic cells using a Tunel kit on decalcified mouse > femurs. Not having much success, if anyone has any tips or do's / don'ts > I > would really appreciate the information. Thanks in advance, > > > Amy S. Porter, HT(ASCP) QIHC - Supervisor > Michigan State University > Investigative HistoPathology Laboratory > Department of Physiology / Division of Human Pathology > 2100 Biomedical Physical Sciences Bldg. Room #2133 > East Lansing, MI 48824-3320 > Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 > Email: portera@msu.edu > www.humanpathology.msu.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________ NOD32 1.1766 (20060921) Information __________ > > This message was checked by NOD32 antivirus system. > http://www.eset.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tbraud <@t> holyredeemer.com Fri Sep 22 15:31:39 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Sep 22 15:33:04 2006 Subject: [Histonet] Histotechnology Laboratory Procedure Assignment Message-ID: in answer to your questions... 1. For the whole lab, CAP(College of American Pathologists), JCAHO (Joint Commission for Accreditation of Healthcare Organizations) and AABB (American Assoc of Blood Banks) Procedures must be reviewed annually, divided into sections so that a portion is reviewed every month. New procedures must be reviewed by the Chief Pathologist, and thereafter, by his designee (in this case, the Anatomic Pathology Supervisor) This is a CAP reg. If a new Medical Director is hired, then he/she must review and sign all lab procedures within a reasonable amount of time. 2. We have 322 Histology related procedures. Those pertaining to all divisions of the lab (such as retention of records, HIPPA rules, etc.) are in the Gen Lab Manual in addition to all Safety Procedures that are in a General Lab Safety manual Specific to the AP department, we have 4 manuals. The General Anatomic Pathology manual, which covers Histology and Cytology, the Immunohistochemistry Manual, the Special Stains Manual, and the Quality Assurance and Quality Control Manual. In addition to that, we keep a manual of MSDS (Material Safety Data Sheets) for every chemical in use in our department. 3. New employees must show review of the manuals by signature, in addition to signing a proficiency qualification for all procedures they are expected to routinely perform. They must be signed off on any procedure by a supervising technician before performing the procedure independently. After that, certain procedures are selectively tested for annual competancy for the annual review. Also, any technician performing tissue gross dissections must have a special annual gross competancy check by the chief pathologist for every type of specimen that is defined in the non pathologist grossing policy. These cases are directly observed, documented, and signed off on by the Chief Pathologist in order for them to perform gross dissections. 4. The AP Supervisor is responsible for the creation of new procedures as requested or as needed. They must be written in NCCLS (National Committee for Clinical Laboratory Standards)approved format, and they must be approved by the Chief Pathologist and then signed off by all technicians before being put into place. I hope this helps. Terri Braud Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Medical Laboratory Holy Redeemer Hospital and Medical Center (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scheherazade Humphrey Sent: Friday, September 22, 2006 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnology Laboratory Procedure Assignment Hello Everyone, My name is Scheherazade Humphrey and I am currently a student at Argosy University in the Twin Cities. I was asked by my instructor to post a listserve to get possible answers and help on one of my assignments. If any one is able to answer any of the following questions, that would be greatly appreciated. Thank you. The following questions are : 1. What accreditation does your laboratory have? Who is responsible for reviewing your lab procedures? How often is it done? 2. About how many histotechnology related procedures exist in your laboratory? How are they organized? Where are they kept? 3. What is the policy for new employees in relation to learning the laboratory procedures? 4. Who is responsible for creating new procedures when needed? What is the process? Thank you for participating in my Questionnaire for my Histotechnology Laboratory Procedure Assignment. Scheherazade H. --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From liz <@t> premierlab.com Fri Sep 22 16:44:06 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Sep 22 16:39:49 2006 Subject: [Histonet] Yeh glad to be back & ?? In-Reply-To: <002901c6de82$b48e0980$8e7a0923@HistoJJ> Message-ID: <000e01c6de90$3ac8c190$0300a8c0@domain.Premier> Amy I use cleaved caspase 3 from cell signaling technologies, it does require HIER retrieval, which you can do on bone but you are going to have some section loss. I'll attach my protocol in an additional e-mail. I run lots of immunos on formic acid decalicifed bone samples with good success. I try to stick with enzyme retrieval either pronase or proteinase K. Proteinase K is my favorite. What other antibodies are you looking at? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: Amy Porter [mailto:portera@msu.edu] Sent: Friday, September 22, 2006 2:07 PM To: Patsy Ruegg; 'Liz Chlipala'; 'Histonet' Subject: Re: [Histonet] Yeh glad to be back & ?? Thanks Patsy - Are you using Caspase 3 ? or another Caspase for that and is it active or cleaved - just curious. I think I am going to have trouble all the way around. We are trying to do immuno on mouse tibias that have been acid decalcified. After several postings I am trying to look at alternative decalcification methods, I have been able to obtain some forelimbs from mice just to work with the decaling and processing. Any pointers that you would have would be greatly appreciated. Amy ----- Original Message ----- From: "Patsy Ruegg" To: "'Liz Chlipala'" ; "'Amy Porter'" ; "'Histonet'" Sent: Friday, September 22, 2006 3:43 PM Subject: RE: [Histonet] Yeh glad to be back & ?? > Amy, > I agree with Liz on this, it is hard enough with Tunnel to distinguish > between necrosis and apoptosis without adding decal. I much prefer > caspase, > you can count on the positive cells being just those under going > apoptosis. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz > Chlipala > Sent: Thursday, September 21, 2006 9:45 AM > To: 'Amy Porter'; 'Histonet' > Subject: RE: [Histonet] Yeh glad to be back & ?? > > Amy > > I did this back a few years ago and found that the process of formic acid > decalcification caused all the nuclei to be positive. From what I > remember > the use of formic acid is not compatible with the tunel technique. Why > don't you try using cleaved caspase 3 immunohistochemistry instead. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter > Sent: Thursday, September 21, 2006 7:09 AM > To: Histonet > Subject: [Histonet] Yeh glad to be back & ?? > > Is anyone out there doing Tunel on FFPE acid decalcifed sections. I am > trying to stain for apoptotic cells using a Tunel kit on decalcified mouse > femurs. Not having much success, if anyone has any tips or do's / don'ts > I > would really appreciate the information. Thanks in advance, > > > Amy S. Porter, HT(ASCP) QIHC - Supervisor > Michigan State University > Investigative HistoPathology Laboratory > Department of Physiology / Division of Human Pathology > 2100 Biomedical Physical Sciences Bldg. Room #2133 > East Lansing, MI 48824-3320 > Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 > Email: portera@msu.edu > www.humanpathology.msu.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________ NOD32 1.1766 (20060921) Information __________ > > This message was checked by NOD32 antivirus system. > http://www.eset.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > __________ NOD32 1.1768 (20060922) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From bomeara <@t> globalscience.co.nz Fri Sep 22 17:01:04 2006 From: bomeara <@t> globalscience.co.nz (bomeara@globalscience.co.nz) Date: Fri Sep 22 17:00:56 2006 Subject: [Histonet] Barbara O'Meara/NZ/GlobalScience is out of the office. Message-ID: I will be out of the office starting 22/09/2006 and will not return until 27/09/2006. I will respond to your message when I return, or contact our Auckland office global@globalscience.co.nz From jamubiru <@t> yahoo.com Fri Sep 22 17:21:59 2006 From: jamubiru <@t> yahoo.com (James Mubiru) Date: Fri Sep 22 17:22:07 2006 Subject: [Histonet] Honey as a fixative? Message-ID: <20060922222159.23950.qmail@web54715.mail.yahoo.com> Dear all I saw an article in the Journal of Histotechnology on honey as a fixative and was impressed by the innovative spirit of the authors. It would be helpful to the whole histo community if more studies are done on honey as a fixative. The main question should be if the results are reproducible by other investigators using honey from different areas. It would be also interesting to know the component of honey that is responsible for the fixation. James Mubiru --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From jnocito <@t> satx.rr.com Sat Sep 23 05:13:09 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Sep 23 05:13:17 2006 Subject: [Histonet] off-topic re: our Tim Morken References: <01c501c6de7d$b58e7bd0$a1065486@auxs.umn.edu> Message-ID: <005601c6def8$df054cc0$63614542@yourxhtr8hvc4p> ahhh. The price of fame. Go Tim Go ----- Original Message ----- From: "Jan Shivers" To: "histonet" Sent: Friday, September 22, 2006 1:33 PM Subject: [Histonet] off-topic re: our Tim Morken I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.405 / Virus Database: 268.12.8/455 - Release Date: 9/22/2006 From jqb7 <@t> cdc.gov Sat Sep 23 06:21:05 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Sat Sep 23 06:24:00 2006 Subject: [Histonet] off-topic re: our Tim Morken References: <01c501c6de7d$b58e7bd0$a1065486@auxs.umn.edu> Message-ID: Okay, inquiring minds and all that stuff......how old was the picture???? Sorry, Tim. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/22/2006 2:33 PM To: histonet Cc: Subject: [Histonet] off-topic re: our Tim Morken I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From josephnerk <@t> hotmail.com Sat Sep 23 08:21:30 2006 From: josephnerk <@t> hotmail.com (Joseph Nerk) Date: Sat Sep 23 08:21:37 2006 Subject: [Histonet] Quality Assurance Program Message-ID: Hello once again Can anyone provide information on where I could find some for of Quality Assurance Program in Histotechnology, _________________________________________________________________ Express yourself instantly with MSN Messenger! [1]MSN Messenger Download today it's FREE! References 1. http://g.msn.com/8HMBEN/2740??PS=47575 From carl.hobbs <@t> kcl.ac.uk Sat Sep 23 12:55:04 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Sep 23 12:55:50 2006 Subject: [Histonet] re: Honey as a fixative Message-ID: No ..it is a preservative, not a fixative. Tim Morken does in fact mention this en passant. However, just thought I?d underline the fact. Carl From sheila_adey <@t> hotmail.com Sat Sep 23 15:01:31 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sat Sep 23 15:01:42 2006 Subject: [Histonet] flame Dako In-Reply-To: Message-ID: I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don’t waste time standing in line—try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca From Pathrm35 <@t> adelphia.net Sat Sep 23 16:46:08 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Sat Sep 23 16:44:02 2006 Subject: [Histonet] flame Dako In-Reply-To: Message-ID: <000001c6df59$adfa8e40$2ea2ac45@D6WRV2B1> I miss my old Biogenex i6000!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Saturday, September 23, 2006 4:02 PM To: mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sun Sep 24 13:25:00 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sun Sep 24 13:25:14 2006 Subject: [Histonet] RE: flame Dako Message-ID: I would never ?flame ? anyone. There are better ways to deal with problems, if one is prepared to spend the time. I have: I have always used and championed Dako?s immunodetection reagents , until recently. I just got fed up paying a LOT of money for their detection reagents when Vectorlabs deliver the same reagents for much less outlay. Sure, I had to spend a week recalibrating. So I no longer buy Dako. They were such a great , tactile, Company in the early days. Even Behemoths like VWR still attempt to contact customers more often than Dako ..these days. Since I left clinical labs 10yrs ago, I have never, ever, been personally contacted by any DAKO Rep. Also, all of the PIs in our Centre do not even bother with Dako such is their non-existant profile in our College. Does this constitute a ?flame? .? ;-) Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases Kings College London England. From sohail_e <@t> yahoo.com Sun Sep 24 13:32:50 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Sun Sep 24 13:32:55 2006 Subject: [Histonet] Tracheal whole mount staining Message-ID: <20060924183250.70422.qmail@web39511.mail.mud.yahoo.com> Dear All I am currently screening DKK1 transgenic mice for their effect on tracheal vasculature using the whole mount immunoflouresence. I am using Cd31 antibody for that and everything is going well when i do it with retinal whole mount staining but when it comes to trachea, i cant see any blood vessels. Is there anyone who could kindly guide me a complete protocol for whole mount immunoflouresence of trachea. Thanks Dr.Sohail Ejaz histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. re: Honey as a fixative (Carl Hobbs) 2. RE: flame Dako (sheila adey) 3. RE: flame Dako (Ron Martin) ---------------------------------------------------------------------- Message: 1 Date: Sat, 23 Sep 2006 18:55:04 +0100 From: "Carl Hobbs" Subject: [Histonet] re: Honey as a fixative To: "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" No ..it is a preservative, not a fixative. Tim Morken does in fact mention this en passant. However, just thought I?d underline the fact. Carl ------------------------------ Message: 2 Date: Sat, 23 Sep 2006 16:01:31 -0400 From: "sheila adey" Subject: RE: [Histonet] flame Dako To: mauger@email.chop.edu, Histonet@pathology.swmed.edu, DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Message-ID: Content-Type: text/plain; format=flowed I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don?t waste time standing in line?try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca ------------------------------ Message: 3 Date: Sat, 23 Sep 2006 17:46:08 -0400 From: "Ron Martin" Subject: RE: [Histonet] flame Dako To: "'sheila adey'" , , , Cc: MAGILJ@pathology.ufl.edu Message-ID: <000001c6df59$adfa8e40$2ea2ac45@D6WRV2B1> Content-Type: text/plain; charset="us-ascii" I miss my old Biogenex i6000!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Saturday, September 23, 2006 4:02 PM To: mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 34, Issue 29 **************************************** --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From AnthonyH <@t> chw.edu.au Sun Sep 24 17:58:59 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 24 18:00:25 2006 Subject: [Histonet] The infamous honey paper Message-ID: I'm surprised that more people are not a member of the NSH since you would have received the journal where the paper was published. I can't recommend this journal highly enough for those with a professional interest in Histotechnology. Membership is very cheap and even with the current exchange rate being poor, we in Australia make sure we receive it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tamara A Howard Sent: Saturday, 23 September 2006 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The infamous honey paper Would someone be willing/able to send me a pdf of this paper? Our library does not have a subscription and I'd like to see what all the fuss (I'll avoid saying "buzz") is about. Thanks! *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Sep 24 18:02:38 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 24 18:04:10 2006 Subject: [Histonet] t writing summary on honey as a fixative and othercomments Message-ID: But how much of the preservation is due to alcohol in the processing of the blocks to wax. It would be interesting to observe the central shrinkage in tissues such as spleen and lymph node that often occurs in underfixed tissue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Saturday, 23 September 2006 4:05 AM To: histonet@pathology.swmed.edu Subject: [Histonet] t writing summary on honey as a fixative and othercomments Honey for use for anything is a popular subject in the Arab peninsula (one of the authors of this paper is of apparent Arab descent). In Arabia honey is considered a food, a preservative for food and medical substance used for treatment of various ailments. Some consider it a practically magical elixer. When I worked in Saudi Arabia our med tech / histotech students constantly wanted to do studies using honey for one thing or another so when I saw this topic I instantly knew the impetus for it. This current article may actually be informative if it is considered in the context of preserving tissue for other uses, not necessarily for high quality histology work. I know that is not the authors intention, but research in one field often informs another field without intending to. I do agree that the reviewers should have been much more critical of the authors contention that the histology of honey- vs formalin-fixed tissue is comparable (describe to what degree it is comparable). It is obvious the pictures were chosen to prove their point. For instance, the picture of a formalin-fixed rat kidney glomerulus is the worst I have ever seen (tissue is badly distorted) while the honey-fixed tissue is reasonable for gross structure. Even so, it does show that honey does preserve tissue to a cetain degree which may be useful for something. The fact a certain honey was used is probably not too critical for this study, but honey from different areas do have different chemicals in them depending on the flowers the nectar came from. Tim Morken Gayle, I agree this paper is interesting it has us all talking about it. I have my own bees and so does my neighbor and I can get plenty of spare tissues so I think I'm going to repeat the experiment. I didn't have much honey this year but I'll use next years and compare with local honey and some purchased from the grocery store. I'll try some on perfused rats and try some C.N.S. Tissues this should be fun. It will be an interesting topic at the Maryland Beekeepers Meeting. I'll let everyone know what happens. Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Pain and Neurobiology Branch On 9/21/06 4:50 PM, "Gayle Callis" wrote: > Dawn, > > Have your student contact the author of this publication for further > discussion on this topic. I also suggest your student access some of the > references cited in the publication - it would be a good exercise for doing > a reference i.e. literature search to broaden their knowledge base. > > Philip.Bryant@bromor-tr.wales.nhs.uk > > Although honey may not be an ideal formalin substitute and the honey was > from two species of bees found in Oman, I was intrigued by the topic both > how it was used in the paper and historically as a preservative and > dehydrant in other parts of the world. I found the publication unique > and rather interesting that something like this can be done, maybe without > the better results of formalin fixation. I also saw the poster at 2006 NSH > S/C, where many were just as curious as I was honey as a formalin > substitute, it was a popular poster to visit. > > If people are NOT happy with the publication as it is, a letter to the JOH > editor would be in order and allow the authors to make further comments on > what points may have been not addressed as they may not be looking in on > Histonet to see current commentary or critique of their work. > > > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) Tim Morken Product Development Lab Vision - Neomarkers 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 email: tpmorken@labvision.com web: www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Annette_hall <@t> pa-ucl.com Sun Sep 24 21:37:08 2006 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Sun Sep 24 21:37:18 2006 Subject: [Histonet] flame Dako Message-ID: <9FC023A4AB52BB4D87DC6456081A822C070C87@mercury.pa-ucl.com> We also have the Artisan and constantly struggle to keep it running. Everything from antibodies, diluents, TRIS buffer, and detection kits have been on back order. Makes you wonder if Dako bought the competition (Cytologix) just to eliminate it. They certainly aren't supporting it, or us. -----Original Message----- From: sheila adey [mailto:sheila_adey@hotmail.com] Sent: Saturday, September 23, 2006 3:02 PM To: mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sackmanj <@t> gmail.com Sun Sep 24 21:49:09 2006 From: sackmanj <@t> gmail.com (Joseph Sackman) Date: Sun Sep 24 21:49:14 2006 Subject: [Histonet] Mounting and staining placenta Message-ID: Im looking for the best technique for cutting (microtome), mounting, and staining rat placenta to look at structure.I plan on using an H&E stain, but I'm not sure which one is the best. Most papers state they use a standard H&E stain; but even still there seems to be several types and techniques. Does anyone have any suggestions? -Joe From ree3 <@t> leicester.ac.uk Mon Sep 25 04:25:14 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Sep 25 04:25:36 2006 Subject: [Histonet] 18.5 to 9.5!!. In-Reply-To: Message-ID: From JWEEMS <@t> sjha.org Mon Sep 25 07:02:55 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Sep 25 07:03:19 2006 Subject: [Histonet] flame Dako Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEB8C@sjhaexc02.sjha.org> I've used DAKO products for a 100 years and fully believe in the company. In their defense, they upgraded their computer system, and it has been a nightmare. Be patient and they will be back to normal before long. Keep in touch with your rep - if you don't have one that pays attention, then call DAKO and let them know. Happy Monday! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Annette Hall Sent: Sunday, September 24, 2006 10:37 PM To: 'sheila adey'; mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako We also have the Artisan and constantly struggle to keep it running. Everything from antibodies, diluents, TRIS buffer, and detection kits have been on back order. Makes you wonder if Dako bought the competition (Cytologix) just to eliminate it. They certainly aren't supporting it, or us. -----Original Message----- From: sheila adey [mailto:sheila_adey@hotmail.com] Sent: Saturday, September 23, 2006 3:02 PM To: mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From oshel1pe <@t> cmich.edu Mon Sep 25 07:25:58 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Mon Sep 25 07:26:14 2006 Subject: [Histonet] Honey as a fixative? In-Reply-To: <20060922222159.23950.qmail@web54715.mail.yahoo.com> References: <20060922222159.23950.qmail@web54715.mail.yahoo.com> Message-ID: I would be very surprised if there is anything in honey that acts as a fixative. Remember that honey is evolved to be food for growing bee larvae, not exactly something compatible with fixation. I do find it easy to believe that honey acts as a preservative, simply because its high sugar content makes honey a strong dehydrating agent. This will perserve tissue and prevent bacterial and fungal growth. It would be interesting to do a similar study on salt-preserved tissues. I suspect the results would be similar, although the morphology would be uglier in the salt-preseved tissue because salt's strong osmotic effects. Phil >Dear all > I saw an article in the Journal of Histotechnology on honey as a >fixative and was impressed by the innovative spirit of the authors. > It would be helpful to the whole histo community if more studies >are done on honey as a fixative. > The main question should be if the results are reproducible by >other investigators using honey from different areas. It would be >also interesting to know the component of honey that is responsible >for the fixation. > James Mubiru -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From prippstein <@t> ottawaheart.ca Mon Sep 25 08:38:29 2006 From: prippstein <@t> ottawaheart.ca (Peter Rippstein) Date: Mon Sep 25 08:39:01 2006 Subject: [Histonet] stents & Spurr epoxy resin Message-ID: Hello histonetters Has anybody out there processed and sectioned any stented arteries in Spurr epoxy resin? If so, I would greatly appreciate a protocol of the method you have been using. Many thanks in advance.... Peter Peter Rippstein ART, MLT Core Pathology Laboratory Rm H2102 University of Ottawa Heart Institute 40 Ruskin Street Ottawa, Ontario Canada, K1Y 4W7 Tel: (613) 761-5282 Fax: (613) 761-5281 Email: prippstein@ottawaheart.ca -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD From Terry.Marshall <@t> rothgen.nhs.uk Mon Sep 25 09:13:43 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Sep 25 09:17:31 2006 Subject: [Histonet] Honey as a fixative? Message-ID: Ah, the voice of reason. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Philip Oshel [mailto:oshel1pe@cmich.edu] Sent: 25 September 2006 13:26 To: Histonet@Pathology.swmed.edu Subject: Re: [Histonet] Honey as a fixative? I would be very surprised if there is anything in honey that acts as a fixative. Remember that honey is evolved to be food for growing bee larvae, not exactly something compatible with fixation. I do find it easy to believe that honey acts as a preservative, simply because its high sugar content makes honey a strong dehydrating agent. This will perserve tissue and prevent bacterial and fungal growth. It would be interesting to do a similar study on salt-preserved tissues. I suspect the results would be similar, although the morphology would be uglier in the salt-preseved tissue because salt's strong osmotic effects. Phil >Dear all > I saw an article in the Journal of Histotechnology on honey as a >fixative and was impressed by the innovative spirit of the authors. > It would be helpful to the whole histo community if more studies >are done on honey as a fixative. > The main question should be if the results are reproducible by >other investigators using honey from different areas. It would be >also interesting to know the component of honey that is responsible >for the fixation. > James Mubiru -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Sep 25 09:21:40 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Sep 25 09:22:06 2006 Subject: [Histonet] honey as a formalin substitute/NEW MOVIE In-Reply-To: <90B6684A9D6DAF468F7A5DC148754E1D05F357@ALPW31.f2.enterprise> Message-ID: Honey, It Fixed The Kids -----Original Message----- From: Stephen.Eyres@sanofi-aventis.com [mailto:Stephen.Eyres@sanofi-aventis.com] Sent: 22 September 2006 10:54 To: ree3@leicester.ac.uk; rjbuesa@yahoo.com; Barry.R.Rittman@uth.tmc.edu; Dawn.Olszewski@SGMC.ORG; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute On the other hand you could ask then to pollen the other one, or your views were nectar to my ears, or then again, this paper seems to be causing a flap!!! I'll stop now, I need a lay down. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 22, 2006 9:28 AM To: Rene J Buesa; Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Perhaps the authors should have been told to buzz off?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 September 2006 18:20 To: Rittman, Barry R; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute Barry R: I cannot agree more with you. You would not have accepted the paper for publication in the form it was accepted neither would have I. Yes, the JOH is peer reviewed but it seems that peers reviewing this paper gave more weight to publishing the paper than to assure quality of the paper and by doing so have jeopardized the stature of the JOH as the quality vehicle it should be not to mentione the inconsistency between this article and others dealing with esoteric techniques frequently published and wonderful topic articles on December of each year. Until I resigned from the Editorial board of the JOH I reviewed some papers, and made recommendations that sometimes were followed and sometimes not. Somebody "drop the ball" at the JOH with this article. Again, just my opinion. Ren? J. "Rittman, Barry R" wrote: Rene I was in process of also commenting on this paper to the Journal but perhaps here is as important. Rene - I agree with all the points that you have written. To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 11:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Sep 25 09:24:49 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Sep 25 09:25:01 2006 Subject: [Histonet] flame Dako In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEB8C@sjhaexc02.sjha.or g> References: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEB8C@sjhaexc02.sjha.org> Message-ID: <6.0.0.22.1.20060925081723.01b58288@gemini.msu.montana.edu> Joyce is right, and in defense of DAKO - we use some DAKO products and when all the problems erupted with their computer update, we contacted our DAKO rep, who advised us to order products by FAX or through him. He advised NO TELEPHONE orders, and probably not order on line either. Some computer glitch created havoc saying things were backordered, when in reality, products were sitting on the shelf. You may not want to CALL DAKO, email them instead. I am not about to give up my beloved DAKO chromogens until Hades freezes over. Good luck At 06:02 AM 9/25/2006, you wrote: >I've used DAKO products for a 100 years and fully believe in the company. >In their defense, they upgraded their computer system, and it has been a >nightmare. > >Be patient and they will be back to normal before long. Keep in touch with >your rep - if you don't have one that pays attention, then call DAKO and >let them know. > >Happy Monday! >Joyce Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ree3 <@t> leicester.ac.uk Mon Sep 25 09:29:59 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Sep 25 09:30:08 2006 Subject: [Histonet] flame Dako In-Reply-To: <6.0.0.22.1.20060925081723.01b58288@gemini.msu.montana.edu> Message-ID: Here hear for DAKO U.K.............. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 25 September 2006 15:25 To: Weems, Joyce; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] flame Dako Joyce is right, and in defense of DAKO - we use some DAKO products and when all the problems erupted with their computer update, we contacted our DAKO rep, who advised us to order products by FAX or through him. He advised NO TELEPHONE orders, and probably not order on line either. Some computer glitch created havoc saying things were backordered, when in reality, products were sitting on the shelf. You may not want to CALL DAKO, email them instead. I am not about to give up my beloved DAKO chromogens until Hades freezes over. Good luck At 06:02 AM 9/25/2006, you wrote: >I've used DAKO products for a 100 years and fully believe in the company. >In their defense, they upgraded their computer system, and it has been >a nightmare. > >Be patient and they will be back to normal before long. Keep in touch >with your rep - if you don't have one that pays attention, then call >DAKO and let them know. > >Happy Monday! >Joyce Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Sep 25 09:42:27 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Sep 25 09:42:42 2006 Subject: [Histonet] The infamous honey paper In-Reply-To: Message-ID: <007501c6e0b0$d3790ef0$6601a8c0@Patsy> I am with you Tony. Even with the recent increase in membership, NSH is the best bargin around, where else can you get the premier Journal for Histotechnology and all the other benefits of NSH membership for such a small fee. www.nsh.org histo@nsh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Sunday, September 24, 2006 4:59 PM To: Tamara A Howard; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The infamous honey paper I'm surprised that more people are not a member of the NSH since you would have received the journal where the paper was published. I can't recommend this journal highly enough for those with a professional interest in Histotechnology. Membership is very cheap and even with the current exchange rate being poor, we in Australia make sure we receive it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tamara A Howard Sent: Saturday, 23 September 2006 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The infamous honey paper Would someone be willing/able to send me a pdf of this paper? Our library does not have a subscription and I'd like to see what all the fuss (I'll avoid saying "buzz") is about. Thanks! *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Sep 25 09:51:30 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Sep 25 09:51:49 2006 Subject: [Histonet] flame Dako..no - no problems here In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C070C87@mercury.pa-ucl.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6DD3@EMAIL.archildrens.org> I have been receiving all of my orders. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Hall Sent: Sunday, September 24, 2006 9:37 PM To: 'sheila adey'; mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako We also have the Artisan and constantly struggle to keep it running. Everything from antibodies, diluents, TRIS buffer, and detection kits have been on back order. Makes you wonder if Dako bought the competition (Cytologix) just to eliminate it. They certainly aren't supporting it, or us. -----Original Message----- From: sheila adey [mailto:sheila_adey@hotmail.com] Sent: Saturday, September 23, 2006 3:02 PM To: mauger@email.chop.edu; Histonet@pathology.swmed.edu; DOOLEEO@shands.ufl.edu Cc: MAGILJ@pathology.ufl.edu Subject: RE: [Histonet] flame Dako I agree totally. We are switching to Biogenex. (i6000) Our job is tough enough with out worrying about having the products we need. Just curious, were you using the Artisan? Thay's what we have. Sheila Adey >From: "Joanne Mauger" >To: , >CC: MAGILJ@pathology.ufl.edu >Subject: [Histonet] flame Dako >Date: Fri, 22 Sep 2006 10:31:39 -0400 > >Lets all flame DAKO! What's the problem? They are discontinuing so many >products, and we have been waiting for months to receive our orders!! I >was trying to be patient, but I have just about had it!! I have switched >to many LabVision products. Get with it, DAKO, or you will lose >customers! > >That feels better, > >Jo Mauger > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From mauger <@t> email.chop.edu Mon Sep 25 10:02:48 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Mon Sep 25 10:03:51 2006 Subject: [Histonet] flame Dako Message-ID: I have always been a strong advocate for DAKO, but I have been loosing my patience after all these months. I really hope they turn it around, but how long can we wait? We have work to do! Jo From oshel1pe <@t> cmich.edu Mon Sep 25 10:10:34 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Mon Sep 25 10:10:51 2006 Subject: [Histonet] Honey as a fixative? In-Reply-To: References: Message-ID: If insufficiently proof-read. Need more coffee ... >Ah, the voice of reason. > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Philip Oshel [mailto:oshel1pe@cmich.edu] >Sent: 25 September 2006 13:26 >To: Histonet@Pathology.swmed.edu >Subject: Re: [Histonet] Honey as a fixative? > > >I would be very surprised if there is anything in honey that acts as >a fixative. Remember that honey is evolved to be food for growing bee >larvae, not exactly something compatible with fixation. >I do find it easy to believe that honey acts as a preservative, >simply because its high sugar content makes honey a strong >dehydrating agent. This will perserve tissue and prevent bacterial >and fungal growth. It would be interesting to do a similar study on >salt-preserved tissues. I suspect the results would be similar, >although the morphology would be uglier in the salt-preseved tissue >because salt's strong osmotic effects. > >Phil > >>Dear all >> I saw an article in the Journal of Histotechnology on honey as a >>fixative and was impressed by the innovative spirit of the authors. >> It would be helpful to the whole histo community if more studies >>are done on honey as a fixative. >> The main question should be if the results are reproducible by >>other investigators using honey from different areas. It would be >>also interesting to know the component of honey that is responsible >>for the fixation. >> James Mubiru > >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >Central Michigan University >024C Brooks Hall >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From jerry.santiago <@t> jax.ufl.edu Mon Sep 25 10:40:15 2006 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Mon Sep 25 10:40:32 2006 Subject: [Histonet] Florida Society for Histotechnology: Call for Abstracts Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF3089165@jaxmail.umc.ufl.edu> The Florida Society for Histotechnology is calling for abstract for the 2007 Meeting. The meeting will be held at the Bahia Mar Beach Resort, Fort Lauderdale, Florida from April 20-22, 2007. The deadline for abstract submission is December 1st, 2006. All abstract are welcomed. We will be revising abstracts in January 2007. Meeting Information will be available in February 2007. All abstracts forms may be downloaded from our website at www.fshgroup.org. Come Visit! Jerry Santiago, BS, HTL(ASCP)QIHC FSH President Shands Jacksonville Pathology-Tumor Analysis Lab 904-244-6149 From rfisher <@t> gbmc.org Mon Sep 25 12:33:44 2006 From: rfisher <@t> gbmc.org (RENEE FISHER) Date: Mon Sep 25 12:34:24 2006 Subject: [Histonet] Help: I heard in a class at the recent NSH that one could acid clean slides to prevent tissue from Message-ID: <20060925T133344Z_09C200070000@gbmc.org> Help: I heard in a class at the recent NSH that one could acid clean slides to prevent tissue from falling off during IHC staining. Has anyone done this? if so is there a prodedure? Thanks, Renee _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From han <@t> synatom.biz Mon Sep 25 12:40:29 2006 From: han <@t> synatom.biz (Hanqing Xie) Date: Mon Sep 25 12:40:34 2006 Subject: [Histonet] ExactAntigen and Antibody Review project Message-ID: <20060925174030.15178.qmail@web308.biz.mail.mud.yahoo.com> Dear Histonet subscribers, With permission from Dr. Linda Margraf, we are sending you this message to introduce our service. ExactAntigen provides gene- and species-specific reagent information. This email is to introduce our Antibody Review service. In this service, antibody information including suppliers, immunological methods, and more are systematically extracted from peer-reviewed publications, and organized to help ExactAntigen visitors find most suitable antibodies. We have so far collected antibody application information for over 1900 genes, and the number is increasing daily. Here are two examples: http://www.exactantigen.com/review/AKT1.html http://www.exactantigen.com/review/BRCA1.html ExactAntigen is a free website and no registration is required. Many of you are experts in immunohistochemistry, we appreciate your corrections, comments, and suggestions. Thanks for your time and consideration. Hanqing Xie, Ph. D. http://www.exactantigen.com/ From tkngflght <@t> yahoo.com Mon Sep 25 13:39:07 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Sep 25 13:39:11 2006 Subject: [Histonet] acid-cleaned slides --a walk down memory lane. In-Reply-To: <20060925T133344Z_09C200070000@gbmc.org> Message-ID: <20060925183908.49250.qmail@web50912.mail.yahoo.com> Renee--a short history of slides in histology....may want to save it for happy hour. Of course there are members on our 'net who can take this story farther back but in the interest of time: Long before you probably knew there was such a thing as a Histologist, slides came to us in boxes full of dust, dirt and oil deposited by the process of breaking them into slides, etc. These were the days of diamond pens, steel knives and there weren't such a thing as 'frost' slides, coverslippers or slide printers other than the techs who didn't write in cursive! We used to acid clean all of our slides before use (usually an acid alcohol or aqueous acid rinse--depending on oil/finger oil contamination--followed by lots of dH2O rinsing & a drying oven). This was before many advancements in our science in the days when you had your morning coffee on TOP of your microtome and albumin was the adhesive of choice. Slide production improved, then came frosted glass tops and then PAINTED colored tops we could use out of the box!!! This was AWESOME. In the late 70s/early '80s someone (anyone know who came up with this?) figured how poly-L-lysine could be used in solution to charge the surface of a slide without interfering with staining the way albumin did. We cleaned our slides again, to prepare them for dipping in poly-L-lysine! What a marvelous thing! Now with incredible high-tech production methods, there are all sorts of specialty slides available, made more consistently than we could ever produce by hand. Our time is saved and the products WORK, leaving time once in a while for a trip to the break room for coffee (most labs still run on ample supplies of coffee and chocolate.) If you were to acid clean any of these treated products, you'd likely remove what you've paid so dearly to obtain--a slide designed to grab and hold on to that tissue!! I didn't attend this year's NSH (disappointing but no one to hold down the fort) so perhaps I missed something and have dated myself unnessecarily. (Waiting with anticipation for Joe's comments on histo-dating :) ) I WOULD like to hear if there is anyone who used to make 2x2 mounts for slide conference still out there??!! LOL! Hope this helps--sorry if it didn't. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From tbraud <@t> holyredeemer.com Mon Sep 25 14:04:44 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Sep 25 14:06:28 2006 Subject: [Histonet] voice recognition Message-ID: Hello Netters: I am interested in hearing feedback (pro or con) from anyone using Dragon Naturally Speaking, Version 9 ONLY, the Medical version, for pathology transcription. Even more so if it is interfaced with CoPath Plus. How did it affect transcription productivity? report turnaround times? were there layoffs? labor reassignments? How well was it received by pathologists? by any technicians/pa performing gross? Are you using the bluetooth feature to be wireless, or what alternative? What was the training time? How much upfront work was needed for installation? Implementation? Who performed this work? How much downtime is there and how is it handled? Inquiring minds want to know. Thanks for any info that you can provide! Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory Holy Redeemer Hospital (215) 938-3689 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From SEGoebel <@t> mdanderson.org Mon Sep 25 14:11:34 2006 From: SEGoebel <@t> mdanderson.org (SEGoebel@mdanderson.org) Date: Mon Sep 25 14:11:42 2006 Subject: [Histonet] images Message-ID: I am looking for good images that could be printable for several antibodies. Does anyone know a good site for this, or do you possibly have images I could use for personal reference? Sarah Elizabeth Goebel, B.A., HT(ASCP) M.D. Anderson Cancer Center Michale E. Keeling Center for Comparative Medicine and Research Bastrop, Texas 78602 From tpmorken <@t> labvision.com Mon Sep 25 14:49:14 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Mon Sep 25 14:49:23 2006 Subject: [Histonet] images In-Reply-To: Message-ID: You can find good images by searching for free papers on pubmed (or google scholar (http://scholar.google.com) though it is surprising that even peer-reviewed papers rarely describe textually the staining pattern they claim is real (they usually just say "such and such cells are postive"). A good source for education and images is ProPath. Dr Miller has a series of newsletters that are very informative: http://www.propathlab.com/immu/newsletter.asp IHC World has an image database but takes awhile to move through. http://www.ihcworld.com/imagegallery/ These are public domain as far as I know. There are lots of other sites with various images, but nothing that really covers the whole range of IHC in pathology in one spot, especially with good descriptions of what is supposed to be stained in the image! Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SEGoebel@mdanderson.org Sent: Monday, September 25, 2006 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] images I am looking for good images that could be printable for several antibodies. Does anyone know a good site for this, or do you possibly have images I could use for personal reference? Sarah Elizabeth Goebel, B.A., HT(ASCP) M.D. Anderson Cancer Center Michale E. Keeling Center for Comparative Medicine and Research Bastrop, Texas 78602 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Mon Sep 25 14:51:11 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Sep 25 14:51:20 2006 Subject: [Histonet] acid-cleaned slides --a walk down memory lane. Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017177AD@lsexch.lsmaster.lifespan.org> 2x2 mounts for slide conferences? You bet! I actually made a few for someone a couple of years ago! And I still have a case of 2x2 glass in my lab. Those were the days when a new shipment of embedding paraffin went first into a big filter-lined funnel on a big flask in the oven, to melt it and filter all the dirt and crud out of the wax before we used it. But first the 2-pound blocks of solid paraffin had to be broken up with a hammer in order to fit in the funnel. After filtering, the clean wax was transferred into the metal pitchers we used to pour it into the embedding molds. Those were kept in the oven too. You used one pitcher of wax until it started to get too cool, then returned it to the oven and took another pitcher. Then on friday afternoon we would all sit on chairs in a circle, fold little paper squares over wooden blocks to make little paper boxes, and toss them into a carton on the floor in the middle of the circle (our embedding molds for the following week). What fun! Paul M. From tpmorken <@t> labvision.com Mon Sep 25 14:54:09 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Mon Sep 25 14:54:15 2006 Subject: [Histonet] off-topic re: our Tim Morken In-Reply-To: Message-ID: Hey, it was only about 4 years ago! Those who have been around awhile might remember a freelance writer posted on Histonet looking for a histotech to interview and to take pictures of in the lab. I volunteered and the rest is history. I even got a free copy of the book! It is "Biology" (high school) by Prentice-Hall publishers and has a dragonfly on the cover. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCID) Sent: Saturday, September 23, 2006 4:21 AM To: Jan Shivers; histonet Subject: RE: [Histonet] off-topic re: our Tim Morken Okay, inquiring minds and all that stuff......how old was the picture???? Sorry, Tim. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/22/2006 2:33 PM To: histonet Cc: Subject: [Histonet] off-topic re: our Tim Morken I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Mon Sep 25 15:47:50 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Sep 25 15:48:25 2006 Subject: [Histonet] acid-cleaned slides --a walk down memory lane. References: <20060925183908.49250.qmail@web50912.mail.yahoo.com> Message-ID: <001101c6e0e3$ddd13070$130e4246@yourlk4rlmsu> Oh, yes, I used to make 2x2 slides, both from mounted sections, stained and coverslipped, and by photographing off a computer screen. The first ones I made were from 2x2 pieces of glass made to look good by covering the edge with tape after the Canada Balsam dried. Later I progressed to the luxury of using glass film mounts for 35 mm with a fancy plastic surround, which occasionally softened from the xylene if I put the glass back too soon. Text slides were made from Kodak lith film developed in a high contrast lith developer from text typed onto white paper, or from Letraset plastic letters. They were colored in bulk by soaking the film in a dilute orange G solution. Highlighting was done with a paint brush and solutions of basic fuchsin, light green etc. It used to take longer to make a series of slides than to write and give the lecture ten times over. I still have some of them stashed away. Once computers with flat screens (relatively) became common I used to prepare the text on a black screen, with fancy fonts and coloured text and photograph with a macro lens (otherwise used for flowers) for 2 seconds so the scan lines would not show. The last talk I gave requiring projection was about a year ago and I used a series of web pages and a browser with a computer projector and screen. It took all the fun out of it! About adhesive slides; I never used to buy them as I always found a really clean slide caused the tissue to adhere quite well in nearly all cases. However, I did notice that the outward facing surface of the first and last slides in a box were often incredibly sticky. This was the surface in contact with the plastic film wrap. The slides were those most inexpensive ground edge slides made in Germany. I used to save them for those special cases we all love. Once the tissue touched the slide it could not be moved, i.e. no repositioning allowed. The only way to get the section off the slide was to scrub it with tissue paper. The scrubbing did not remove the sticky surface no matter how hard I pressed, and the slide could be reused. I always presumed there was some deposit transferred from whatever material the plastic film was made from. Whatever it was, it was the most effective adhesive I ever came accross. Bryan Llewellyn ----- Original Message ----- From: "Cheryl" To: "RENEE FISHER" ; Sent: Monday, September 25, 2006 11:39 AM Subject: [Histonet] acid-cleaned slides --a walk down memory lane. > Renee--a short history of slides in histology....may want to save it for > happy hour. Of course there are members on our 'net who can take this > story farther back but in the interest of time: > > Long before you probably knew there was such a thing as a Histologist, > slides came to us in boxes full of dust, dirt and oil deposited by the > process of breaking them into slides, etc. These were the days of diamond > pens, steel knives and there weren't such a thing as 'frost' slides, > coverslippers or slide printers other than the techs who didn't write in > cursive! We used to acid clean all of our slides before use (usually an > acid alcohol or aqueous acid rinse--depending on oil/finger oil > contamination--followed by lots of dH2O rinsing & a drying oven). This > was before many advancements in our science in the days when you had your > morning coffee on TOP of your microtome and albumin was the adhesive of > choice. > > Slide production improved, then came frosted glass tops and then PAINTED > colored tops we could use out of the box!!! This was AWESOME. In the > late 70s/early '80s someone (anyone know who came up with this?) figured > how poly-L-lysine could be used in solution to charge the surface of a > slide without interfering with staining the way albumin did. We cleaned > our slides again, to prepare them for dipping in poly-L-lysine! What a > marvelous thing! > > Now with incredible high-tech production methods, there are all sorts of > specialty slides available, made more consistently than we could ever > produce by hand. Our time is saved and the products WORK, leaving time > once in a while for a trip to the break room for coffee (most labs still > run on ample supplies of coffee and chocolate.) If you were to acid clean > any of these treated products, you'd likely remove what you've paid so > dearly to obtain--a slide designed to grab and hold on to that tissue!! > > I didn't attend this year's NSH (disappointing but no one to hold down > the fort) so perhaps I missed something and have dated myself > unnessecarily. (Waiting with anticipation for Joe's comments on > histo-dating :) ) I WOULD like to hear if there is anyone who used to > make 2x2 mounts for slide conference still out there??!! LOL! > > Hope this helps--sorry if it didn't. > > Cheryl > > > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one Tech at a time. > 281.883.7704 c > 281.852.9457 o > admin@fullstaff.org > > Sign up for the FREE monthly newsletter AP News--updates, tricks of the > trade and current issues for Anatomic Pathology Clinical Labs. Send a > 'subscribe' request to APNews@fullstaff.org. Please include your name and > specialty in the body of the email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotecnologia <@t> hotmail.com Mon Sep 25 16:43:04 2006 From: histotecnologia <@t> hotmail.com (Colegio de Histotecnologos) Date: Mon Sep 25 16:43:16 2006 Subject: [Histonet] problems with leica microtome Message-ID: Hello all!! I read about the thik and thin sections problems. I have a 2125 leica microtome. ??ve been having the same problem with mine. I changed the leaf spring, the clamping lever and the clamps, three times, it start working fine and after a few moth it brokes, the leaf spring wear down and start working bad again. Does any one now if there is a recall for those knife holders?? Its wird that everyone is having the same problem. isn?t? Thanks HT Anaeva Aleo Caracas Venezuela _________________________________________________________________ Las mejores tiendas, los precios mas bajos, entregas en todo el mundo, YupiMSN Compras: [1]Haz clic aqu?... References 1. http://g.msn.com/8HMBES/2746??PS=47575 From lidiaz <@t> ucsd.edu Mon Sep 25 16:57:15 2006 From: lidiaz <@t> ucsd.edu (Diaz, Liliana) Date: Mon Sep 25 16:59:18 2006 Subject: [Histonet] Microwave processor question. Message-ID: <550F625770202C4CBE4EF3296F0805A91C39C6@ucsdhc-exch4.AD.UCSD.EDU> Hello Histonet members. I am a current employee at UCSan Diego. We want to purchase a microwave processor and would like some good and bad feedback about current microwave tissue processors available in the market. Thanks for your help, Lily From Bauer.Karen <@t> mayo.edu Mon Sep 25 18:57:29 2006 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Mon Sep 25 18:58:09 2006 Subject: [Histonet] Histology Opening in Eau Claire, WI Message-ID: Job Opportunity - Histology Luther Midelfort-Mayo Health System (Eau Claire, WI) is seeking a full-time Histotechnician. The position is 32-40 hrs/wk, varied shifts 5am - 5:30pm, and every 5th wknd. Responsibilities include: accession, grossing, embedding, cutting, coverslipping, histochemical and immunoperoxidase staining, maintenance, data entry, QA/QC, and filing. The Histology Department consists of a team oriented group that is highly efficient, yet flexible enough to be accommodating. Luther Midelfort's pathology laboratory is a comprehensive medical laboratory offering both clinical and anatomic laboratory services. The laboratory has a staff of 95 technical and support employees, four board certified clinical and anatomic pathologists and is accredited by the College of American Pathologists. The laboratory provides inpatient, outpatient, and outreach services in West-Central Wisconsin. For more information, please view the job posting at www.luthermidelfort.org . Thank You. ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From vanann702 <@t> skmc.gov.ae Mon Sep 25 23:41:54 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Mon Sep 25 23:40:06 2006 Subject: [Histonet] flame Dako Message-ID: Our DAKO vendor is great, and so is the local DAKO service engineer. We have no problems getting our orders - even out here in the desert!!! Annie in Abu Dhabi -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: 25 September 2006 18:30 To: Gayle Callis; Weems, Joyce; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] flame Dako Here hear for DAKO U.K.............. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 25 September 2006 15:25 To: Weems, Joyce; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] flame Dako Joyce is right, and in defense of DAKO - we use some DAKO products and when all the problems erupted with their computer update, we contacted our DAKO rep, who advised us to order products by FAX or through him. He advised NO TELEPHONE orders, and probably not order on line either. Some computer glitch created havoc saying things were backordered, when in reality, products were sitting on the shelf. You may not want to CALL DAKO, email them instead. I am not about to give up my beloved DAKO chromogens until Hades freezes over. Good luck At 06:02 AM 9/25/2006, you wrote: >I've used DAKO products for a 100 years and fully believe in the company. >In their defense, they upgraded their computer system, and it has been >a nightmare. > >Be patient and they will be back to normal before long. Keep in touch >with your rep - if you don't have one that pays attention, then call >DAKO and let them know. > >Happy Monday! >Joyce Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jschwamborn <@t> yahoo.de Tue Sep 26 00:55:48 2006 From: jschwamborn <@t> yahoo.de (Jens Schwamborn) Date: Tue Sep 26 00:56:00 2006 Subject: [Histonet] Questions concerning: Mouse Brain Cryosections Message-ID: <20060926055548.98416.qmail@web26808.mail.ukl.yahoo.com> Dear Histonet-people, I want to image EGFP fluorescence (endogenous) in cryosections of embryonic mouse brains (stages: E12.5 to E18.5). Furthermore I would like to perform antibody-stainings for immunofluorescence on those sections. Now I am searching for the best protocol to do so. Do you fix the brains before the sections? If yes, how? (I guess perfusion is impossible at E12.5) Or do you freeze the complete brain, perform the sectioning and fix afterwards? If so, how do you freeze, with dry ice ? Thanks for the help. Jens email: jschwamborn@yahoo.de --------------------------------- Was ist Gl?ck? Schlafen Fische ?berhaupt? Die Antworten gibt?s auf Yahoo! Clever. From Jean.Gillson <@t> elht.nhs.uk Tue Sep 26 05:49:02 2006 From: Jean.Gillson <@t> elht.nhs.uk (Gillson Jean (ELHT) Pathology) Date: Tue Sep 26 05:47:43 2006 Subject: [Histonet] control material Message-ID: <95C57EA9EF5C5A41AC7E075734B75259E42BD4@elht-exch2.xelht.nhs.uk> Hi all, we are very desperate for control material for tuberculosis (ZN stain, toludine blue) and CEA immunocychemistry. If any one can help by providing us with material or directing me to a commercial supplier then I would be extremely grateful. Thankyou Jean From ereagan2005 <@t> aol.com Tue Sep 26 06:41:43 2006 From: ereagan2005 <@t> aol.com (ereagan2005@aol.com) Date: Tue Sep 26 06:41:55 2006 Subject: [Histonet] mohs Message-ID: <8C8AF77DFBD559B-155C-2682@MBLK-M17.sysops.aol.com> i am a mohs histotech and would like to know if anyone knows the limit as to how many cases are acceptable in a day is there a limit to say no thats to much when you are the only histotech doing all the work? i'm only 30 and already have lost the use of my forfinger the only reason anyone has come up with yet is that i have my hand in -26-30 temps for at least 8-10 hours a day. ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Philip.Bryant <@t> bromor-tr.wales.nhs.uk Tue Sep 26 07:02:55 2006 From: Philip.Bryant <@t> bromor-tr.wales.nhs.uk (Philip Bryant) Date: Tue Sep 26 07:03:16 2006 Subject: [Histonet] Honey as a formalin substitute Message-ID: <000E6144BE0F3D4B8E93A92BDA5B900D0949B9@exchpow01.BAN.xbromor.wales.nhs.uk> Now that all the jokes and puns about honey have been exhausted, I thought that I would thank everyone for their interest and comments, positive or otherwise, regarding my publication. When the paper was written, it was intended as a basic, wacky and off the wall idea. It certainly proved to be that and I'm grateful for the publicity it generated. I would also be pleased to hear any other comments or results of any independent research that may come as a result. Apparently, the supermarkets in the UK are finding it hard to compete with the demand for honey and fresh liver!!!! By the way, do you know about the decalcifying powers of Coca Cola.......?? Happy researching! Phil Phil Bryant PhD CSci FIBMS Cellular Pathology Team Manager Department of Pathology Princess of Wales Hospital Bridgend Mid Glamorgan Wales, UK CF31 1RQ Phone : 01656 752320 E-mail : philip.bryant@bromor-tr.wales.nhs.uk Cymraeg:- Mae'r neges hon yn gyfrinachol.Os nad chi yw'r derbynnydd y bwriedid y neges ar ei gyfer, byddwch mor garedig ? rhoi gwybod i'r anfonydd yn ddi-oed. Dylid ystyried un rhywd datganiadau neu sylwadau a wneir uchod yn rhai personol,ac nid o angen rhaid yn rhai o eiddo Ymddiriedolaeth GIG Bro Morgannwg, nac unrhyw ran gyfansoddol ohoni na chorff cysylltiedig. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Bro Morgannwg roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau'r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Bro Morgannwg ar www.bromor-tr.wales.nhs.uk English:- This message is confidential. If you are not the intended recipient of the message then please notify the sender immediately. Any of the statements or comments made above should be regarded as personal and not necessarily those of Bro Morgannwg NHS Trust, any constituent part or connected body. Please be aware that, under the terms of the Freedom of Information Act 2000, Bro Morgannwg NHS Trust may be required to make public the content of any emails or correspondence received. For further information on Freedom of Information, please refer to the Bro Morgannwg NHS Trust website at www.bromor-tr.wales.nhs.uk. From sheila_adey <@t> hotmail.com Tue Sep 26 07:12:11 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Sep 26 07:12:25 2006 Subject: [Histonet] Fatty unfixed tissues? Message-ID: Hi Everyone, Just wondering if anyone has any neat ideas about what to do with fatty tissue that comes out of the processor not processed. We have done the "squeeze" technique and then re soak in paraffin. That helps but any other ideas? It's one of those pathologists who has to put everything in the day it arrives and is not a thin slicer. Thanks in advance Sheila _________________________________________________________________ Don’t waste time standing in line—try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca From Karen.Heckford <@t> CHW.edu Tue Sep 26 07:21:07 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue Sep 26 07:21:24 2006 Subject: [Histonet] Tissue in Paraffin Message-ID: Good Morning my fellow Histonetters, I was wondering how long you would leave tissue unembedded after pulling them out of paraffin. My processor is broke down. I would greatly appreciate the feedback. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From MDiCarlo <@t> KaleidaHealth.Org Tue Sep 26 07:49:53 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Tue Sep 26 07:50:03 2006 Subject: [Histonet] Help: I heard in a class at the recent NSH that onecould acid clean slides to prevent tissue from In-Reply-To: <20060925T133344Z_09C200070000@gbmc.org> Message-ID: <9B4A77DF11463E4FB723D484214AE9BC01AFB524@KALEXMB02.KaleidaHealth.org> Renee, To acid clean slides: 1% Acid Alcohol- 5 minutes Two rinses- 95% alcohol- 5 min. ea. Dry in 60 degree oven for 1 hour or longer. Cool. I usually wrap the slides individually with kimwipes or paper towels- depending on size and store in a box until ready to use. Peggy DiCarlo HT (ASCP) Orthopaedic Bone Lab Buffalo General Hospital 100 High Street Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Monday, September 25, 2006 13:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help: I heard in a class at the recent NSH that onecould acid clean slides to prevent tissue from Help: I heard in a class at the recent NSH that one could acid clean slides to prevent tissue from falling off during IHC staining. Has anyone done this? if so is there a prodedure? Thanks, Renee ________________________________________________________________________ _______________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From vanann702 <@t> skmc.gov.ae Tue Sep 26 07:53:03 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Tue Sep 26 07:51:11 2006 Subject: [Histonet] Fatty unfixed tissues? Message-ID: Sounds like your pathologist needs a few lessons in the 'dos and donts' of grossing. Garbage in garbage out. Now if I was the tech in charge, here is what I would do - and HAVE done....give him sections from the garbage he has grossed. Quietly reprocess the blocks, cut and stain them and then present him with what he 'could' have if he follows certain golden grossing rules. They are all in such a flipping hurry and the quality of the end result gets compromised for a 'neat' turn around time - drives me bananas Today we had staples in some blocks and my techs were bleating. I told them to cut what they could and submit 'shredded' sections to the Path. Have not had a single complaint from him about the sections. He knows he should have checked for the staples! Pathologists need to be trained - well perhaps not ALL of them!! That's my 2 cents worth from out here in the desert Annie in Abu Dhabi -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: 26 September 2006 16:12 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty unfixed tissues? Hi Everyone, Just wondering if anyone has any neat ideas about what to do with fatty tissue that comes out of the processor not processed. We have done the "squeeze" technique and then re soak in paraffin. That helps but any other ideas? It's one of those pathologists who has to put everything in the day it arrives and is not a thin slicer. Thanks in advance Sheila _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Tue Sep 26 08:31:32 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Tue Sep 26 08:32:09 2006 Subject: [Histonet] Sentinel node protocol Message-ID: Good morning, At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would like to know the IHC sentinel protocols performed in other labs. Thanks Rena Fail From sheila_adey <@t> hotmail.com Tue Sep 26 08:45:16 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Sep 26 08:45:21 2006 Subject: [Histonet] Sentinel node protocol In-Reply-To: Message-ID: Hi Rena, We only do the AE1/ AE3 on our sentinels. Sheila >From: "Mildred Fail" >To: >Subject: [Histonet] Sentinel node protocol >Date: Tue, 26 Sep 2006 09:31:32 -0400 > >Good morning, > At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would >like to know the IHC sentinel protocols performed in other labs. Thanks > >Rena Fail > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata=081805 From sheila_adey <@t> hotmail.com Tue Sep 26 09:01:50 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Sep 26 09:01:59 2006 Subject: [Histonet] Tissue in Paraffin In-Reply-To: Message-ID: Hi Karen, I would just put them back into liquid paraffin and if it was at the end of the paraffin infiltration time just go ahead and embed them. Sorry about your processor, I feel your pain :( Sheila >From: "Heckford, Karen - SMMC-SF" >To: "Histonet (E-mail)" >Subject: [Histonet] Tissue in Paraffin >Date: Tue, 26 Sep 2006 05:21:07 -0700 > >Good Morning my fellow Histonetters, I was wondering how long you would >leave tissue unembedded after pulling them out of paraffin. My processor >is broke down. I would greatly appreciate the feedback. >Cheers, >Karen Heckford HT (ASCP) CE >Lead Histology Technician >Histology/Pathololgy Department >St. Mary's Medical Center >450 Stanyan St. >San Francisco, Ca. 94117 >415-668-1000 ext. 6167 >Fax: 415-750-8123 >email: kheckfor@chw.edu >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don’t waste time standing in line—try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca From tbraud <@t> holyredeemer.com Tue Sep 26 09:07:04 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Sep 26 09:08:31 2006 Subject: [Histonet] Sentinel node protocol Message-ID: Same here, but for breast ca sentinel nodes only, for melanomas, we use s-100 and HMB-45. Lev 1 - H&E Lev 2 - Immuno Lev 3 - H&E Lev 4 - Immuno Lev 5 - H&E Lev 6 - Pt negative Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory Holy Redeemer Hospital (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of sheila adey Sent: Tuesday, September 26, 2006 9:45 AM To: failm@musc.edu; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sentinel node protocol Hi Rena, We only do the AE1/ AE3 on our sentinels. Sheila >From: "Mildred Fail" >To: >Subject: [Histonet] Sentinel node protocol >Date: Tue, 26 Sep 2006 09:31:32 -0400 > >Good morning, > At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would >like to know the IHC sentinel protocols performed in other labs. Thanks > >Rena Fail > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata=081805 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From STapper <@t> slhduluth.com Tue Sep 26 09:13:27 2006 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Tue Sep 26 09:13:38 2006 Subject: [Histonet] Bone Marrow H&E Message-ID: I am looking for protocols for bone marrow H&E. If you are willing to send your protocol - fill my box!! Thanks! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN From gcallis <@t> montana.edu Tue Sep 26 09:15:16 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Sep 26 09:15:29 2006 Subject: [Histonet] mohs In-Reply-To: <8C8AF77DFBD559B-155C-2682@MBLK-M17.sysops.aol.com> References: <8C8AF77DFBD559B-155C-2682@MBLK-M17.sysops.aol.com> Message-ID: <6.0.0.22.1.20060926080126.01b4ac80@gemini.msu.montana.edu> Maybe some repetive motion also. To keep my hands warm for long periods of time inside a cryostat, I buy silk gloves or liners from Wintersilks (they have a website),, they come in sizes and are for right and left hand fits. I obviously wear these underneath my protective latex or nitrile gloves. I do not lose dexterity with this layering, but be very careful, wearing gloves that are TOO TIGHT will cut your circulation and contribute to loss of sensation in your fingers. Be sure to find the best glove for YOU, not something that is just supplied willy nilly. Reevaluate how you do your cryotomy, even how you sit at the cryostat to be relaxed and do some stretching, motion exercises of you hands, fingers, wrists, arms and back to relieve both cold and stiffness one encounters for long periods of time inside a cryostat. Sounds like you need to restore your circulation frequently during the day, especially during a cutting session. 8 to 10 hours seems a bit excessive. I would hope you get routine breaks from this kind of repetive work. I also suggest your MOHS surgeon consider hiring another person to help, what you are experiencing my lead to some serious down time and physical therapy or not being able to work at all, then the surgeon would be in a terrible situation if they cannot do their work. You are the third MOHS tech I have heard of who has some type of physical problem due to their work, the others had shoulder, back, neck and rotator cuff problems, and were always going to therapy to alleviate the problem. I was appalled by their cryotomy technics, but most of all, the cryostat they used - it was a horror to watch. Automated cryostats can help do trimming, and keep your hands out of the chamber. At 05:41 AM 9/26/2006, you wrote: >i am a mohs histotech and would like to know if anyone knows the limit as >to how many cases are acceptable in a day is there a limit to say no thats >to much when you are the only histotech doing all the work? i'm only 30 >and already have lost the use of my forfinger the only reason anyone has >come up with yet is that i have my hand in -26-30 temps for at least 8-10 >hours a day. >________________________________________________________________________ >Check out the new AOL. Most comprehensive set of free safety and security >tools, free access to millions of high-quality videos from across the web, >free AOL Mail and more. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From dmccaig <@t> ckha.on.ca Tue Sep 26 09:12:17 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Sep 26 09:18:13 2006 Subject: [Histonet] Sentinel node protocol Message-ID: Hi I would like to know if anyone is handling the sentinal node immediately, doing frozen on them, or putting them in a lead lined tower for 24 hours prior to handling. Thanks Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mildred Fail Sent: Tuesday, September 26, 2006 9:32 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Sentinel node protocol Good morning, At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would like to know the IHC sentinel protocols performed in other labs. Thanks Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fawn <@t> cs.cmu.edu Tue Sep 26 09:18:55 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Tue Sep 26 09:19:04 2006 Subject: [Histonet] Help with immuno Message-ID: <451936CF.7050400@cs.cmu.edu> I was wondering if anybody had an immuno staining procedure that uses the following antibodies: primary, secondary linked to alkaline phosphatase I have a post doc here who wants to do an immuno stain on some mouse femurs embedded in MMA, but she doesn't want to order any kits or antibodies. She uses the antibodies above to do immuno's on cells, and wants to use them on the bone. If anybody could help me out I would greatly appreciate it Fawn From sheila_adey <@t> hotmail.com Tue Sep 26 09:23:48 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Sep 26 09:24:01 2006 Subject: [Histonet] Sentinel node protocol In-Reply-To: Message-ID: Hi Diana, We do a frozen on our Sentinels and then process them that day. Our Radiology dept. determined that the small amount of radioactivity found in the nodes did not warrant any wait times. Hope this helps Sheila >From: "Diana McCaig" >To: "Mildred Fail" , >Subject: RE: [Histonet] Sentinel node protocol >Date: Tue, 26 Sep 2006 10:12:17 -0400 > > Hi > >I would like to know if anyone is handling the sentinal node >immediately, doing frozen on them, or putting them in a lead lined tower >for 24 hours prior to handling. >Thanks >Diana > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mildred >Fail >Sent: Tuesday, September 26, 2006 9:32 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Sentinel node protocol > >Good morning, > At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would >like to know the IHC sentinel protocols performed in other labs. Thanks > >Rena Fail > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Buy what you want when you want it on Sympatico / MSN Shopping http://shopping.sympatico.msn.ca/content/shp/?ctId=2,ptnrid=176,ptnrdata=081805 From bayoubelle311 <@t> gmail.com Tue Sep 26 09:25:15 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Tue Sep 26 09:25:22 2006 Subject: [Histonet] WBC counts Message-ID: <398f02c20609260725v7b8ae2c3u229f2a72c5a9bc9b@mail.gmail.com> Hi all... We have never done a manual WBC count before and my advisor has asked me to get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's stained peripheral blood smears. What is the general protocol for a manual count? Do you count WBC's inb relation to RBCs or just WBCs, the whole slide, or just representative sections? Melissa P.S - this is for rat sample, not human From DavidH <@t> marketlabinc.com Tue Sep 26 09:36:19 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Tue Sep 26 09:36:31 2006 Subject: [Histonet] WBC counts Message-ID: <19E3602A16438E48B51A4250CA04B5F686CB17@exchange.marketlab.com> Missy, Is what your supervisor wants a WBC Differential? If so you count 100 cells identifying each different type of cell and report the percentage of each cell type. A white blood cell count would have to be performed on a whole blood specimen - not a peripheral smear - there is no way to measure volume otherwise as cell counts are reported as number of cells per volume measured. Hope this helps. Dave Haagsma MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Missy Sent: Tuesday, September 26, 2006 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] WBC counts Hi all... We have never done a manual WBC count before and my advisor has asked me to get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's stained peripheral blood smears. What is the general protocol for a manual count? Do you count WBC's inb relation to RBCs or just WBCs, the whole slide, or just representative sections? Melissa P.S - this is for rat sample, not human _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Sep 26 10:07:56 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Sep 26 10:08:39 2006 Subject: [Histonet] Sentinel node protocol In-Reply-To: References: Message-ID: <45190A0C02000077000021A1@hcnwgwds01.hh.chs> We do a cytokeratin cocktail (clones AE1/AE3 and CAM5.2) on one tissue section prepared from blocks that do not show metastatic carcinoma on the frozen section and the permanent section. Are you doing a cocktail or each cytokeratin antibody individually? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Mildred Fail" 09/26/06 9:31 AM >>> Good morning, At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would like to know the IHC sentinel protocols performed in other labs. Thanks Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Sep 26 10:15:09 2006 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Tue Sep 26 10:15:37 2006 Subject: [Histonet] Honey as a formalin substitute Message-ID: Hi Phil, it was good to see and speak with you in Phoenix. now about that Coca Cola, do you know if tissues decal'd in this solution are suitable for immunohistochemistry ?? we never seem to have a shortage of Diet Coke here in the lab, might be a good substitute in a pinch ! 8>) Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Philip Bryant" 09/26/06 08:02AM >>> Now that all the jokes and puns about honey have been exhausted, I thought that I would thank everyone for their interest and comments, positive or otherwise, regarding my publication. When the paper was written, it was intended as a basic, wacky and off the wall idea. It certainly proved to be that and I'm grateful for the publicity it generated. I would also be pleased to hear any other comments or results of any independent research that may come as a result. Apparently, the supermarkets in the UK are finding it hard to compete with the demand for honey and fresh liver!!!! By the way, do you know about the decalcifying powers of Coca Cola.......?? Happy researching! Phil Phil Bryant PhD CSci FIBMS Cellular Pathology Team Manager Department of Pathology Princess of Wales Hospital Bridgend Mid Glamorgan Wales, UK CF31 1RQ Phone : 01656 752320 E-mail : philip.bryant@bromor-tr.wales.nhs.uk Cymraeg:- Mae'r neges hon yn gyfrinachol.Os nad chi yw'r derbynnydd y bwriedid y neges ar ei gyfer, byddwch mor garedig ? rhoi gwybod i'r anfonydd yn ddi-oed. Dylid ystyried un rhywd datganiadau neu sylwadau a wneir uchod yn rhai personol,ac nid o angen rhaid yn rhai o eiddo Ymddiriedolaeth GIG Bro Morgannwg, nac unrhyw ran gyfansoddol ohoni na chorff cysylltiedig. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Bro Morgannwg roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau'r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Bro Morgannwg ar www.bromor-tr.wales.nhs.uk English:- This message is confidential. If you are not the intended recipient of the message then please notify the sender immediately. Any of the statements or comments made above should be regarded as personal and not necessarily those of Bro Morgannwg NHS Trust, any constituent part or connected body. Please be aware that, under the terms of the Freedom of Information Act 2000, Bro Morgannwg NHS Trust may be required to make public the content of any emails or correspondence received. For further information on Freedom of Information, please refer to the Bro Morgannwg NHS Trust website at www.bromor-tr.wales.nhs.uk. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Sep 26 10:26:50 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Sep 26 10:26:57 2006 Subject: AW: [Histonet] Sentinel node protocol In-Reply-To: Message-ID: <001e01c6e180$3070cc40$eeeea8c0@SERVER01> If the sentinel comes in for a frozen section, half of it is done for the frozen and half of it is saved for the paraffin-process. We cut three fozen-levels. With breast-sentinels we do one HE -first section- and one HE - last section-, between we cut in 250 ?m steps. These sections are stained with anti-Pancytokeratin. Melanoma-sentinels are cut in the same way and stained with Pan-Melanoma-Cocktail. Recently we heard about a quick-frozen-stain for Pancytokeratin with the Envision-detection kit, and want to try it soon. If someone can give me input on it, i will be glad. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mildred Fail Gesendet: Dienstag, 26. September 2006 15:32 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sentinel node protocol Good morning, At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would like to know the IHC sentinel protocols performed in other labs. Thanks Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 26 10:26:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 26 10:27:06 2006 Subject: [Histonet] Fatty unfixed tissues? In-Reply-To: Message-ID: <20060926152654.48621.qmail@web61219.mail.yahoo.com> Sheila: Other than good fixation (enough amount and time), thin slices and a correct processing protocol, you will keep having this problem for ever! Ah, and the patien is the one who will be affected! Ren? J. sheila adey wrote: Hi Everyone, Just wondering if anyone has any neat ideas about what to do with fatty tissue that comes out of the processor not processed. We have done the "squeeze" technique and then re soak in paraffin. That helps but any other ideas? It's one of those pathologists who has to put everything in the day it arrives and is not a thin slicer. Thanks in advance Sheila _________________________________________________________________ Don?t waste time standing in line?try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From failm <@t> musc.edu Tue Sep 26 10:28:21 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Tue Sep 26 10:39:52 2006 Subject: [Histonet] Sentinel node protocol Message-ID: each cytokeratin individually Rena Fail >>> "Richard Cartun" 09/26/06 11:07AM >>> We do a cytokeratin cocktail (clones AE1/AE3 and CAM5.2) on one tissue section prepared from blocks that do not show metastatic carcinoma on the frozen section and the permanent section. Are you doing a cocktail or each cytokeratin antibody individually? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Mildred Fail" 09/26/06 9:31 AM >>> Good morning, At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would like to know the IHC sentinel protocols performed in other labs. Thanks Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Philip.Bryant <@t> bromor-tr.wales.nhs.uk Tue Sep 26 10:50:24 2006 From: Philip.Bryant <@t> bromor-tr.wales.nhs.uk (Philip Bryant) Date: Tue Sep 26 10:50:32 2006 Subject: [Histonet] Fatty unfixed tissues Message-ID: <000E6144BE0F3D4B8E93A92BDA5B900D0949C4@exchpow01.BAN.xbromor.wales.nhs.uk> I sent this to Sheila Adey in response to her query and thought it may be of use elsewhere. Try this technique: (1) Trim specimens as usual, dry and place into cassettes(2) Place into solution (Alcohol 600ml, Xylene 300ml and Glacial acetic acid 100ml) for 4 hours, but 2-3 hours may suffice (3) Wash in water (4) Place cassettes into formalin and process as usual. Cheers, Phil Phil Bryant PhD CSci FIBMS Cellular Pathology Team Manager Department of Pathology Princess of Wales Hospital Bridgend, Wales,UK CF31 1RQ Phone : 01656 752320 E-mail : philip.bryant@bromor-tr.wales.nhs.uk Cymraeg:- Mae'r neges hon yn gyfrinachol.Os nad chi yw'r derbynnydd y bwriedid y neges ar ei gyfer, byddwch mor garedig ? rhoi gwybod i'r anfonydd yn ddi-oed. Dylid ystyried un rhywd datganiadau neu sylwadau a wneir uchod yn rhai personol,ac nid o angen rhaid yn rhai o eiddo Ymddiriedolaeth GIG Bro Morgannwg, nac unrhyw ran gyfansoddol ohoni na chorff cysylltiedig. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Bro Morgannwg roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau'r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Bro Morgannwg ar www.bromor-tr.wales.nhs.uk English:- This message is confidential. If you are not the intended recipient of the message then please notify the sender immediately. Any of the statements or comments made above should be regarded as personal and not necessarily those of Bro Morgannwg NHS Trust, any constituent part or connected body. Please be aware that, under the terms of the Freedom of Information Act 2000, Bro Morgannwg NHS Trust may be required to make public the content of any emails or correspondence received. For further information on Freedom of Information, please refer to the Bro Morgannwg NHS Trust website at www.bromor-tr.wales.nhs.uk. From tpmorken <@t> labvision.com Tue Sep 26 11:20:56 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Tue Sep 26 11:21:04 2006 Subject: [Histonet] WBC counts In-Reply-To: <398f02c20609260725v7b8ae2c3u229f2a72c5a9bc9b@mail.gmail.com> Message-ID: Is this some kind of project for a class? If not, hand it over to hematology. If so, get a medical technology text book and look up blood cell counting. To do it right requires special counting slides that are etched with grids. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Missy Sent: Tuesday, September 26, 2006 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] WBC counts Hi all... We have never done a manual WBC count before and my advisor has asked me to get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's stained peripheral blood smears. What is the general protocol for a manual count? Do you count WBC's inb relation to RBCs or just WBCs, the whole slide, or just representative sections? Melissa P.S - this is for rat sample, not human _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo20 <@t> hotmail.com Tue Sep 26 11:37:16 2006 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Tue Sep 26 11:37:25 2006 Subject: [Histonet] Decal for Immunos Message-ID: Hi everyone, Does anyone know of a decal solution without HCL? It seems our RapidCal is interfering with some of the immuno stains. Thanks so much! Paula Wilder St. Joseph Medical Center Towson, MD 21204 From Rae.Staskiewicz <@t> Illinois.gov Tue Sep 26 11:39:38 2006 From: Rae.Staskiewicz <@t> Illinois.gov (Staskiewicz, Rae) Date: Tue Sep 26 11:40:11 2006 Subject: [Histonet] The Illinois Society for Histotechnologists presents a Fall Seminar Message-ID: <0909DF46D9192E45A16DC6254AA853D41E918D@IL084EX102.Illinois.gov> The Illinois Society for Histotechnologists is presenting it's First Annual Fall Seminar, Saturday October 21, 2006. It will be held from 8:00 am to 4:30 pm at the Four Points by Sheraton in Fairview Heights, IL. Topics include Chemical Safety, Tumor Nomenclature, Large Intestine Resections, and Interpreting Special Stains. Speakers include Maureen Doran HTL(ASCP), Steven Nichols MD, Beth Obertino-Norwood MT(ASCP)PA, and M. Lamar Jones BS HT(ASCP). There will be 6 hours of CEU's available. Registration is $20 in advance and $25 the day of the Seminar. Registration includes lunch. You may register on-line at www.ilhisto.org , or by e-mailing dspears@ventanamed.com for a brochure. Hope to see you there! Rae Ann Staskiewicz From gu.lang <@t> gmx.at Tue Sep 26 11:48:08 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Sep 26 11:48:12 2006 Subject: AW: [Histonet] Fatty unfixed tissues In-Reply-To: <000E6144BE0F3D4B8E93A92BDA5B900D0949C4@exchpow01.BAN.xbromor.wales.nhs.uk> Message-ID: <002101c6e18b$8ba11010$eeeea8c0@SERVER01> How does this procedure influence immunhistochemistry of breast tissue? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Philip Bryant Gesendet: Dienstag, 26. September 2006 17:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fatty unfixed tissues I sent this to Sheila Adey in response to her query and thought it may be of use elsewhere. Try this technique: (1) Trim specimens as usual, dry and place into cassettes(2) Place into solution (Alcohol 600ml, Xylene 300ml and Glacial acetic acid 100ml) for 4 hours, but 2-3 hours may suffice (3) Wash in water (4) Place cassettes into formalin and process as usual. Cheers, Phil Phil Bryant PhD CSci FIBMS Cellular Pathology Team Manager Department of Pathology Princess of Wales Hospital Bridgend, Wales,UK CF31 1RQ Phone : 01656 752320 E-mail : philip.bryant@bromor-tr.wales.nhs.uk Cymraeg:- Mae'r neges hon yn gyfrinachol.Os nad chi yw'r derbynnydd y bwriedid y neges ar ei gyfer, byddwch mor garedig ? rhoi gwybod i'r anfonydd yn ddi-oed. Dylid ystyried un rhywd datganiadau neu sylwadau a wneir uchod yn rhai personol,ac nid o angen rhaid yn rhai o eiddo Ymddiriedolaeth GIG Bro Morgannwg, nac unrhyw ran gyfansoddol ohoni na chorff cysylltiedig. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Bro Morgannwg roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau'r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Bro Morgannwg ar www.bromor-tr.wales.nhs.uk English:- This message is confidential. If you are not the intended recipient of the message then please notify the sender immediately. Any of the statements or comments made above should be regarded as personal and not necessarily those of Bro Morgannwg NHS Trust, any constituent part or connected body. Please be aware that, under the terms of the Freedom of Information Act 2000, Bro Morgannwg NHS Trust may be required to make public the content of any emails or correspondence received. For further information on Freedom of Information, please refer to the Bro Morgannwg NHS Trust website at www.bromor-tr.wales.nhs.uk. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Sep 26 11:56:03 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Sep 26 11:57:09 2006 Subject: [Histonet] off-topic re: our Tim Morken References: Message-ID: <00a301c6e18c$a7071c90$a1065486@auxs.umn.edu> In his textbook photo, Tim looks very professional, and handsome, of course. And I can attest that as far as 10th grade biology books go, this one is Great. It even has a section on Stem Cells in it. I find myself reading the darn thing for pleasure... Oh geeeez, am I officially becoming a biology nerd? Jan ----- Original Message ----- From: "Morken, Tim" To: "histonet" Sent: Monday, September 25, 2006 2:54 PM Subject: RE: [Histonet] off-topic re: our Tim Morken Hey, it was only about 4 years ago! Those who have been around awhile might remember a freelance writer posted on Histonet looking for a histotech to interview and to take pictures of in the lab. I volunteered and the rest is history. I even got a free copy of the book! It is "Biology" (high school) by Prentice-Hall publishers and has a dragonfly on the cover. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCID) Sent: Saturday, September 23, 2006 4:21 AM To: Jan Shivers; histonet Subject: RE: [Histonet] off-topic re: our Tim Morken Okay, inquiring minds and all that stuff......how old was the picture???? Sorry, Tim. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/22/2006 2:33 PM To: histonet Cc: Subject: [Histonet] off-topic re: our Tim Morken I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Sep 26 12:10:28 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Sep 26 12:10:35 2006 Subject: [Histonet] Coca-cola: decalcify and cryoprotect? Message-ID: Dear All: If Coca-cola does decalcify, there may be another advantage. How much sugar is in Coke? It may do two things, decalcify and be a cryoprotectant! We prepare our decalcified tissues for freezing and cryostat sectioning by placing them in sucrose, so why not combine two steps in one? Anyone willing to try it? Let me know the results! Sarah Pixley Ohio From tpmorken <@t> labvision.com Tue Sep 26 12:35:22 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Tue Sep 26 12:35:28 2006 Subject: [Histonet] off-topic re: our Tim Morken In-Reply-To: <00a301c6e18c$a7071c90$a1065486@auxs.umn.edu> Message-ID: If interested you can see the page of the Biology book at the California Society site under the Downloads section: http://www.californiahistology.org/index.cfm?p=10 Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, September 26, 2006 9:56 AM To: histonet Subject: Re: [Histonet] off-topic re: our Tim Morken In his textbook photo, Tim looks very professional, and handsome, of course. And I can attest that as far as 10th grade biology books go, this one is Great. It even has a section on Stem Cells in it. I find myself reading the darn thing for pleasure... Oh geeeez, am I officially becoming a biology nerd? Jan ----- Original Message ----- From: "Morken, Tim" To: "histonet" Sent: Monday, September 25, 2006 2:54 PM Subject: RE: [Histonet] off-topic re: our Tim Morken Hey, it was only about 4 years ago! Those who have been around awhile might remember a freelance writer posted on Histonet looking for a histotech to interview and to take pictures of in the lab. I volunteered and the rest is history. I even got a free copy of the book! It is "Biology" (high school) by Prentice-Hall publishers and has a dragonfly on the cover. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCID) Sent: Saturday, September 23, 2006 4:21 AM To: Jan Shivers; histonet Subject: RE: [Histonet] off-topic re: our Tim Morken Okay, inquiring minds and all that stuff......how old was the picture???? Sorry, Tim. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/22/2006 2:33 PM To: histonet Cc: Subject: [Histonet] off-topic re: our Tim Morken I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue Sep 26 12:53:20 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Sep 26 12:53:37 2006 Subject: [Histonet] shipping regs Message-ID: <8C8AFABC986B3F2-F70-270B@MBLK-M42.sysops.aol.com> Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From godsgalnow <@t> aol.com Tue Sep 26 12:53:20 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Sep 26 12:53:40 2006 Subject: [Histonet] shipping regs Message-ID: <8C8AFABC986B3F2-F70-270B@MBLK-M42.sysops.aol.com> Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From akbitting <@t> geisinger.edu Tue Sep 26 13:23:45 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Sep 26 13:24:01 2006 Subject: [Histonet] Antigen retrieval in the microwave Message-ID: <451937F1020000C9000001DC@GHSGWIANW5V.GEISINGER.EDU> I want to make sure I'm, totally CAP compliant, soooooo here's my question. Has anyone gotten cited for heating their citrate buffer or EDTA (for antigen retrieval) in an unvented microwave? I'm getting the impression that CAP wants all microwaves vented even though they throw out that blurb about "unnecessary if only non-hazardous solutions are being heated, i.e. water, some biological stains, paraffin sections". Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From rjbuesa <@t> yahoo.com Tue Sep 26 13:27:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 26 13:27:21 2006 Subject: [Histonet] shipping regs In-Reply-To: <8C8AFABC986B3F2-F70-270B@MBLK-M42.sysops.aol.com> Message-ID: <20060926182718.50851.qmail@web61224.mail.yahoo.com> If you ask either FedEx of UPS they will inform you because they are required by law to follow all existing regulations. Ren? J. godsgalnow@aol.com wrote: Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From rjbuesa <@t> yahoo.com Tue Sep 26 13:27:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 26 13:27:27 2006 Subject: [Histonet] shipping regs In-Reply-To: <8C8AFABC986B3F2-F70-270B@MBLK-M42.sysops.aol.com> Message-ID: <20060926182718.50851.qmail@web61224.mail.yahoo.com> If you ask either FedEx of UPS they will inform you because they are required by law to follow all existing regulations. Ren? J. godsgalnow@aol.com wrote: Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From rjbuesa <@t> yahoo.com Tue Sep 26 13:31:08 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 26 13:31:17 2006 Subject: [Histonet] Antigen retrieval in the microwave In-Reply-To: <451937F1020000C9000001DC@GHSGWIANW5V.GEISINGER.EDU> Message-ID: <20060926183108.41542.qmail@web61218.mail.yahoo.com> Nobody that I know off. Citrate buffer falls into the classification of non-hazardous solutions (0.1 M conc. solutions). Ren? J. Angela Bitting wrote: I want to make sure I'm, totally CAP compliant, soooooo here's my question. Has anyone gotten cited for heating their citrate buffer or EDTA (for antigen retrieval) in an unvented microwave? I'm getting the impression that CAP wants all microwaves vented even though they throw out that blurb about "unnecessary if only non-hazardous solutions are being heated, i.e. water, some biological stains, paraffin sections". Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From pmarcum <@t> vet.upenn.edu Tue Sep 26 13:36:43 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Sep 26 13:36:52 2006 Subject: [Histonet] Pennsylvania Histotechnology Society Fall Symposium Message-ID: <6.1.1.1.2.20060926141510.019b61e8@mail.vet.upenn.edu> Welcome back for those who attended NSH! Welcome to those still needing CEUs for the year and those who could not attend NSH! We are presenting our Fall Symposium on October 26th (Mangement Seminars for All), October 27th and 28th will be workshops and seminars for histologists an related fields. The hotel is booking fast and we have over 30 vendors for you to meet during the meeting! Join us by checking out the program at www.pahisto.org the official PHS web site, print it off and use the handy registration form at the back to insure your place in the workshops we are offering. Let your colleagues and friends know about the meeting! We invite anyone who would like to attend and we have marketed to all of Region II (Delaware, Maryland, New Jersey, Pennsylvania and Virginia) as well as Ohio, New York and West Virginia and of course Washington DC. Please the web site at www.pahisto.org (just so you don't miss it) for abstracts and full speaker information. SEE YOU IN OCTOBER IN PITTSBURGH PA!! This day is designed for Supervisors and Managers to assist in some of the special areas we are all asked to become experts in today with limited resources and opportunities to attend local educational events. If you are a new manager or just need a refresher with updates join us!! WS# 1 8:00AM to 11:30AM CPT Coding - Which Code Do I Use? Whitaker WS# 2 1:00PM to 4:30PM Preparing for a CAP Inspection Nocito & Hernandez WS# 3 4:30PM to 6:30PM The SWEET Workshop Smart Working Environment Ergonomics Training Minshew Friday Oct 27 - Morning Sessions WS# 4 8:00AM to 9:30 AM Richmond Introduction to Fine Needle Aspiration for the Histotechnologist WS# 5 10:00AM to 11:30 AM Histology Laboratory Workload Measurement: Evaluating Complexity and Productivity Schmitt WS# 6 8:00AM to 11:30 AM Don?t Let Immunos Intimidate You! Whitaker Beginning Immunos and Refresher WS# 7 8:00AM to 9:30 AM Safety a Priority in Our Lives Casey WS# 8 10:00AM to 11:30 AM Recycling for Histology Welch WS# 9 8:00AM to 11:30AM Praet Unlocking the Secrets of Mohs? Grossing and Cryosectioning Friday October 27 ? Afternoon Sessions WS#10 1:00PM to 2:30 PM Smart Shopping and Contracts for Histology Macrea WS# 11 1:00PM to 2:30 PM Detection and Amplification of Nucleic Acids in Morphologically Preserved Cells and Tissues Gore WS# 12 1:00PM to 2:30PM Automated Stains Workshop with Multiple Vendors (15 minutes each) WS# 13 3:00PM to 4:30 PM The Bog People of Northern Europe Olsen WS#14 1:00PM to 4:30 PM Nocito & Grossing Surgical Specimens: A Histologist View Hernandez WS# 15 1:00PM to 4:30 PM Peters Frozen Section Techniques New Methods for Cryo-Embedding Saturday October 28 - Morning Sessions WS#16 8:00AM to 11:30 AM Brave New World: An Introduction to Molecular Pathology Haas WS#17 8:00AM to 11:30 AM Meyers Wet Workshop: Multi-antigen Immunohistochemistry (IHC) Staining WS#18 8:00AM to 11:30 AM The Art of Forensic Autopsy Mancuso WS #19 8:00AM to 4:30AM QIHC Readiness and Troubleshooting Macrea WS# 20 8:00AM to 4:30PM HT (ASCP) Examination Readiness Micciche Saturday October 28 Afternoon Sessions WS# 21 1:00PM to 2:30 PM What Plastic Do I Embed This Project In? Marcum WS# 22 1:00PM to 2:30 PM Specimen Labeling and Identification Bar Codes TBA WS#23 1:00PM to 2:30 PM Forensic Insect Entomology Todaro Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From Kari.Zajic <@t> HCAhealthcare.com Tue Sep 26 13:41:38 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Sep 26 13:41:47 2006 Subject: [Histonet] shipping regs In-Reply-To: <20060926182718.50851.qmail@web61224.mail.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC6C4@ORLEV03.hca.corpad.net> I have only dealt with shipping specimens on dry ice but I know UPS and FedEx both have very different company policies regarding these (in the state of Florida, anyway) shipments. Both of those shipping companies have "dangerous goods" departments that can specifically tell you what you need to do. I think formalin, dry ice and human tissue/products in general are considered "dangerous goods" and require shipper's declaration and the nine yards (i.e.. specific labeling, packaging,etc.). Hope this helps... FedEx Dangerous Goods Dept: 901-344-3000 (M-F 7a-8p) UPS website: www.UPS.com Kari :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Tuesday, September 26, 2006 2:27 PM To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] shipping regs If you ask either FedEx of UPS they will inform you because they are required by law to follow all existing regulations. Ren? J. godsgalnow@aol.com wrote: Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Zajic <@t> HCAhealthcare.com Tue Sep 26 13:41:38 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Sep 26 13:41:49 2006 Subject: [Histonet] shipping regs In-Reply-To: <20060926182718.50851.qmail@web61224.mail.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC6C4@ORLEV03.hca.corpad.net> I have only dealt with shipping specimens on dry ice but I know UPS and FedEx both have very different company policies regarding these (in the state of Florida, anyway) shipments. Both of those shipping companies have "dangerous goods" departments that can specifically tell you what you need to do. I think formalin, dry ice and human tissue/products in general are considered "dangerous goods" and require shipper's declaration and the nine yards (i.e.. specific labeling, packaging,etc.). Hope this helps... FedEx Dangerous Goods Dept: 901-344-3000 (M-F 7a-8p) UPS website: www.UPS.com Kari :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Tuesday, September 26, 2006 2:27 PM To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] shipping regs If you ask either FedEx of UPS they will inform you because they are required by law to follow all existing regulations. Ren? J. godsgalnow@aol.com wrote: Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Tue Sep 26 14:21:22 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Sep 26 14:22:47 2006 Subject: [Histonet] Antigen retrieval in the microwave Message-ID: The trick will be to only use transparent containers, another CAPmania reg...my microwave pressure cooker just got 86'd. I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory Holy Redeemer Hospital (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela Bitting Sent: Tuesday, September 26, 2006 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval in the microwave I want to make sure I'm, totally CAP compliant, soooooo here's my question. Has anyone gotten cited for heating their citrate buffer or EDTA (for antigen retrieval) in an unvented microwave? I'm getting the impression that CAP wants all microwaves vented even though they throw out that blurb about "unnecessary if only non-hazardous solutions are being heated, i.e. water, some biological stains, paraffin sections". Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From jtcrhb <@t> umr.edu Tue Sep 26 14:22:20 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Tue Sep 26 14:23:41 2006 Subject: [Histonet] (no subject) Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5A@UMR-CMAIL1.umr.edu> I do not have an automatic cover slipper therefor I will be coverslipping by hand and I need to know what the easiest way to do this is. I also need to know what coverslipping medium you all would recommend. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 From froyer <@t> bitstream.net Tue Sep 26 14:24:29 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Sep 26 14:24:41 2006 Subject: [Histonet] WBC counts In-Reply-To: Message-ID: <001b01c6e1a1$64c7ae70$7701a80a@Ford> Melissa, It sounds like your advisor wants a "differential" WBC if you are attempting to do it on a stained peripheral blood smear. To do this you would need some type of multi-key manual differential counter (example: http://stores.implex.net/minnesotamedical/product_info.php?cPath=205_174_176 &products_id=1726) To do this you assign a key on the counter to a specific white cell (i.e. nuetrophils) and do the same for the rest of the keys with the different types of white cells. You then scan the slide and count the different white cells (punching the proper key for each) until you reach a total count of 100 cells (pay no attention to the red cells). Your differential would then be the number of specific cells counted express as a percentage. Example, you counted 62 nuetrophils and 38 lymphocytes = 62% neturo & 38% Lymphs respectively. To do a total WBC, you would have to have a hemocytometer (what Tim was talking about), calibrated hemo pipettes, a lysing reagent, normal saline diluent, and WHOLE blood. You would dilute the whole blood to the proper dilution with normal saline, add the lysing reagent to destroy the red cells, load the hemocytometer, and count all the white cells you see. Or, as Tom suggested... give the assignment to the Hematology Department. ;-) Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, September 26, 2006 11:21 AM To: Missy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts Is this some kind of project for a class? If not, hand it over to hematology. If so, get a medical technology text book and look up blood cell counting. To do it right requires special counting slides that are etched with grids. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Missy Sent: Tuesday, September 26, 2006 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] WBC counts Hi all... We have never done a manual WBC count before and my advisor has asked me to get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's stained peripheral blood smears. What is the general protocol for a manual count? Do you count WBC's inb relation to RBCs or just WBCs, the whole slide, or just representative sections? Melissa P.S - this is for rat sample, not human _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Tue Sep 26 14:38:37 2006 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Tue Sep 26 14:38:41 2006 Subject: [Histonet] WBC counts Message-ID: Good luck finding a hemacytometer and/or any calibrated pipettes. Ours ended up in the display of "antique instruments and equipment". That's the best place for them (speaking as an old dinosaur who actually learned how to use them). Jacquie Poteete MT(ASCP)QIHC Lead Medical Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Tuesday, September 26, 2006 2:24 PM To: 'Missy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts Melissa, It sounds like your advisor wants a "differential" WBC if you are attempting to do it on a stained peripheral blood smear. To do this you would need some type of multi-key manual differential counter (example: http://stores.implex.net/minnesotamedical/product_info.php?cPath=205_174 _176 &products_id=1726) To do this you assign a key on the counter to a specific white cell (i.e. nuetrophils) and do the same for the rest of the keys with the different types of white cells. You then scan the slide and count the different white cells (punching the proper key for each) until you reach a total count of 100 cells (pay no attention to the red cells). Your differential would then be the number of specific cells counted express as a percentage. Example, you counted 62 nuetrophils and 38 lymphocytes = 62% neturo & 38% Lymphs respectively. To do a total WBC, you would have to have a hemocytometer (what Tim was talking about), calibrated hemo pipettes, a lysing reagent, normal saline diluent, and WHOLE blood. You would dilute the whole blood to the proper dilution with normal saline, add the lysing reagent to destroy the red cells, load the hemocytometer, and count all the white cells you see. Or, as Tom suggested... give the assignment to the Hematology Department. ;-) Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, September 26, 2006 11:21 AM To: Missy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts Is this some kind of project for a class? If not, hand it over to hematology. If so, get a medical technology text book and look up blood cell counting. To do it right requires special counting slides that are etched with grids. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Missy Sent: Tuesday, September 26, 2006 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] WBC counts Hi all... We have never done a manual WBC count before and my advisor has asked me to get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's stained peripheral blood smears. What is the general protocol for a manual count? Do you count WBC's inb relation to RBCs or just WBCs, the whole slide, or just representative sections? Melissa P.S - this is for rat sample, not human _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 26 14:38:33 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 26 14:38:45 2006 Subject: [Histonet] (no subject) In-Reply-To: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5A@UMR-CMAIL1.umr.edu> Message-ID: <20060926193833.20203.qmail@web61223.mail.yahoo.com> Regarding the medium any commercially available will be good and if it loses fluidity add xylene (even if it is originally dissolved in toluene). As to how to do it best, is like getting to Carnegie Hall: PRACTICE, PRACTICE, PRACTICE. Ren? J. "Campbell, John Thomas (UMR-Student)" wrote: I do not have an automatic cover slipper therefor I will be coverslipping by hand and I need to know what the easiest way to do this is. I also need to know what coverslipping medium you all would recommend. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From jtcrhb <@t> umr.edu Tue Sep 26 14:49:40 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Tue Sep 26 14:49:46 2006 Subject: [Histonet] coverslipping Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5C@UMR-CMAIL1.umr.edu> I just recently sent out an email regarding coverslipping. The question that I asked was What coverslipping medium do you all recomend? I am covering H&E slides. I would also like to know any tips for coverslipping since I will be doing it by hand. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 From kweidenh <@t> montefiore.org Tue Sep 26 15:00:43 2006 From: kweidenh <@t> montefiore.org (Karen Weidenheim) Date: Tue Sep 26 15:00:25 2006 Subject: [Histonet] shipping regs Message-ID: Also see Saf T Pak. at www.saftpak.com, 1 800 814-7484. Anyone who packs and ships specimens has to be trained. Saf T Pak offers courses all over the country, twice a year, also cd roms that you can use at your place, avoiding travel fees. You can have one person take the course, and then train others at your institution. K. Weidenheim Karen M. Weidenheim, M.D. Professor of Pathology, Clinical Neurology and Clinical Neurosurgery Albert Einstein College of Medicine Chief, Division of Neuropathology Montefiore Medical Center 111 East 210th Street Bronx, NY 10467 (718) 920-4446 FAX (718) 653-3409 Beeper (917) 556 3696 CONFIDENTIALITY NOTICE: This email, including any attachments, is for the sole use of the intended recipient(s). The information contained in this message may be private and confidential, and may also be subject to the work product doctrine. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. >>> Rene J Buesa 09/26/06 2:27 PM >>> If you ask either FedEx of UPS they will inform you because they are required by law to follow all existing regulations. Ren? J. godsgalnow@aol.com wrote: Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kweidenh <@t> montefiore.org Tue Sep 26 15:00:43 2006 From: kweidenh <@t> montefiore.org (Karen Weidenheim) Date: Tue Sep 26 15:00:28 2006 Subject: [Histonet] shipping regs Message-ID: Also see Saf T Pak. at www.saftpak.com, 1 800 814-7484. Anyone who packs and ships specimens has to be trained. Saf T Pak offers courses all over the country, twice a year, also cd roms that you can use at your place, avoiding travel fees. You can have one person take the course, and then train others at your institution. K. Weidenheim Karen M. Weidenheim, M.D. Professor of Pathology, Clinical Neurology and Clinical Neurosurgery Albert Einstein College of Medicine Chief, Division of Neuropathology Montefiore Medical Center 111 East 210th Street Bronx, NY 10467 (718) 920-4446 FAX (718) 653-3409 Beeper (917) 556 3696 CONFIDENTIALITY NOTICE: This email, including any attachments, is for the sole use of the intended recipient(s). The information contained in this message may be private and confidential, and may also be subject to the work product doctrine. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. >>> Rene J Buesa 09/26/06 2:27 PM >>> If you ask either FedEx of UPS they will inform you because they are required by law to follow all existing regulations. Ren? J. godsgalnow@aol.com wrote: Does anyone know what the shipping regs are for flying specimens in formalin, ie, what kind of packing is required, etc.? Thanks Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Sep 26 15:07:06 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Sep 26 15:07:39 2006 Subject: [Histonet] coverslipping In-Reply-To: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5C@UMR-CMAIL1.umr.edu> Message-ID: John, It is important to know what you are clearing the slides in. Not all mounting media are compatible with all of the xylene substitutes. Jennifer "Campbell, John Thomas (UMR-Student)" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/26/2006 12:49 PM To "histonet" cc Subject [Histonet] coverslipping I just recently sent out an email regarding coverslipping. The question that I asked was What coverslipping medium do you all recomend? I am covering H&E slides. I would also like to know any tips for coverslipping since I will be doing it by hand. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtcrhb <@t> umr.edu Tue Sep 26 15:13:01 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Tue Sep 26 15:14:18 2006 Subject: [Histonet] coverslipping References: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5C@UMR-CMAIL1.umr.edu> Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5D@UMR-CMAIL1.umr.edu> Lets try this again. I just recently sent out an email regarding coverslipping. The question that I asked was What coverslipping medium do you all recomend? I am covering H&E slides. The slides will be coming out of xylene. I would also like to know any tips for coverslipping since I will be doing it by hand. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 From rdavis4 <@t> rdg.boehringer-ingelheim.com Tue Sep 26 15:17:12 2006 From: rdavis4 <@t> rdg.boehringer-ingelheim.com (rdavis4@rdg.boehringer-ingelheim.com) Date: Tue Sep 26 15:17:33 2006 Subject: [Histonet] coverslipping In-Reply-To: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D5C@UMR-CMAIL1.umr.edu> Message-ID: <83BA2D3D42947D48BDAA449453644ABE086592@RDGEXM01.am.boehringer.com> John, There are many ways to hand coverslip; there is no wrong way, as long as it turns out well. I use Cytoseal-XYL from Richard Allan for hand coverslipping. It is xylene based. It comes in a plastic bottle and you can dispense it directly from the bottle to the coverslip. I choose the correct size slip for the tissue sample, and apply the medium to the slip. (you can also use the one size fits all slips) Then, I place the slide perpendicular to the slip and close it face down. Be sure to have the medium run from one side of the slip to the other, or you will increase your chances of air bubbles. Too much is better than not enough, and with only a few tries you will know how much is enough. Use a Kimwipe to wipe away excess xylene or medium from the back of the slide and place it on a flat to dry. The slides need to stay in xylene until JUST before applying the coverslip. Hope this helps. Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) Toxicology, Histopathology Lab Boehringer-Ingelheim Pharmaceuticals, Inc. rdavis4@rdg.boehringer-ingelheim.com 203-798-5448 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, John Thomas (UMR-Student) Sent: Tuesday, September 26, 2006 3:50 PM To: histonet Subject: [Histonet] coverslipping I just recently sent out an email regarding coverslipping. The question that I asked was What coverslipping medium do you all recomend? I am covering H&E slides. I would also like to know any tips for coverslipping since I will be doing it by hand. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Sep 26 15:22:11 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Sep 26 15:22:24 2006 Subject: [Histonet] WBC counts In-Reply-To: References: Message-ID: <6.0.0.22.1.20060926141141.01b39e80@gemini.msu.montana.edu> We purchase hemocytometers from Fisher (Healthcare catalog) or VWR. We still use them for lung lavage/washings from mice. AND we do cell count aka differentials on Diff Quik (if you can get it without difficulty) stained murine lung lavage/cytospins. If you have to do a differential on animal blood smears, buffy coats, Wright Giemsa stain is superb for this purpose, Harleco brand. For a WBC count, contact a clinical laboratory to run the WBC counts on their automated counters, much more accurate and worth the cost. It was painful to work with the RBC and WBC pipettes - HOORAY for modern technology and instrumentation. At 01:38 PM 9/26/2006, you wrote: >Good luck finding a hemacytometer and/or any calibrated pipettes. Ours >ended up in the display of "antique instruments and equipment". That's >the best place for them (speaking as an old dinosaur who actually >learned how to use them). Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Barry.R.Rittman <@t> uth.tmc.edu Tue Sep 26 15:23:03 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Sep 26 15:23:09 2006 Subject: [Histonet] WBC counts Message-ID: Hi I suspect that all your advisor wants to do is to get the relative amounts of each of the leukocytes. To do this select an area of the blood smear where the red blood cells are separated a little. Avoid clumps where the smear will be thick or the tail ends of the smear where the white cells may be clumped and distorted. Ideally the red blood cell should be salmon pink. If they are red then all the colors that you have in a description of the white cells will be shifted towards the red, if the red blood cells are slate blue then similarly all the colors will be shifted towards the blue. Start by counting the white cells in the first field. Move the slide one field over say to the right. Count these cell types. Then move one field down and count. Then one field to the right and count. Then up one field and count. Repeat this procedure until you have counted at least 200 preferably more leukocytes. If you get to the end of the slide or in an undesirable area during moving then move down one field and repeat this but moving to the left etc. This is the battlement method of counting and will give you a differential count of leukocytes. The more leukocytes you count the more accurate your percentages. You may have trouble in finding basophils as they are only present in the order of 0.5 to 1%. Hope that this helps Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Tuesday, September 26, 2006 2:39 PM To: Ford Royer; Missy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts Good luck finding a hemacytometer and/or any calibrated pipettes. Ours ended up in the display of "antique instruments and equipment". That's the best place for them (speaking as an old dinosaur who actually learned how to use them). Jacquie Poteete MT(ASCP)QIHC Lead Medical Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Tuesday, September 26, 2006 2:24 PM To: 'Missy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts Melissa, It sounds like your advisor wants a "differential" WBC if you are attempting to do it on a stained peripheral blood smear. To do this you would need some type of multi-key manual differential counter (example: http://stores.implex.net/minnesotamedical/product_info.php?cPath=205_174 _176 &products_id=1726) To do this you assign a key on the counter to a specific white cell (i.e. nuetrophils) and do the same for the rest of the keys with the different types of white cells. You then scan the slide and count the different white cells (punching the proper key for each) until you reach a total count of 100 cells (pay no attention to the red cells). Your differential would then be the number of specific cells counted express as a percentage. Example, you counted 62 nuetrophils and 38 lymphocytes = 62% neturo & 38% Lymphs respectively. To do a total WBC, you would have to have a hemocytometer (what Tim was talking about), calibrated hemo pipettes, a lysing reagent, normal saline diluent, and WHOLE blood. You would dilute the whole blood to the proper dilution with normal saline, add the lysing reagent to destroy the red cells, load the hemocytometer, and count all the white cells you see. Or, as Tom suggested... give the assignment to the Hematology Department. ;-) Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, September 26, 2006 11:21 AM To: Missy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts Is this some kind of project for a class? If not, hand it over to hematology. If so, get a medical technology text book and look up blood cell counting. To do it right requires special counting slides that are etched with grids. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Missy Sent: Tuesday, September 26, 2006 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] WBC counts Hi all... We have never done a manual WBC count before and my advisor has asked me to get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's stained peripheral blood smears. What is the general protocol for a manual count? Do you count WBC's inb relation to RBCs or just WBCs, the whole slide, or just representative sections? Melissa P.S - this is for rat sample, not human _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Tue Sep 26 15:44:35 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Tue Sep 26 15:44:44 2006 Subject: [Histonet] Antigen retrieval in the microwave In-Reply-To: References: Message-ID: <45199133.7080904@ebsciences.com> If you'll forgive a vendor for butting in... Regarding the microwave pressure cooker: Let's not confuse "microwave transparency" with what we typically associate with the concept of transparency: /optical/ transparency. They're not the same. Microwave transparency refers to an object's ability to allow passage of microwave energy without converting a significant portion to heat. While some optically transparent objects happen also to be microwave transparent, that's not always true; leaded crystal in a microwave would be a disaster. Conversely, opaque white Teflon isn't something we'd think of as "transparent," yet it IS "microwave transparent." EBS has just produced a "Microwave Companion for Histology," a 20-page booklet that seeks to put to rest just this kind of confusion, and it's free for the asking. It's purely informational, not looking to sell anything, with a view towards "advancing the art" rather than "shameless commerce." Please contact me directly and I'll be happy to send you one, or a few. Best regards, Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com Terri Braud wrote: > The trick will be to only use transparent containers, another CAPmania reg...my microwave pressure cooker just got 86'd. I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale. Terri > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supv. > Laboratory > Holy Redeemer Hospital > (215) 938-3689 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela > Bitting > Sent: Tuesday, September 26, 2006 2:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Antigen retrieval in the microwave > > > I want to make sure I'm, totally CAP compliant, soooooo here's my > question. > Has anyone gotten cited for heating their citrate buffer or EDTA (for > antigen retrieval) in an unvented microwave? > I'm getting the impression that CAP wants all microwaves vented even > though they throw out that blurb about "unnecessary if only > non-hazardous solutions are being heated, i.e. water, some biological > stains, paraffin sections". > > > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it is addressed. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail at the electronic address shown. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From froyer <@t> bitstream.net Tue Sep 26 15:49:46 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Sep 26 15:49:55 2006 Subject: [Histonet] WBC counts In-Reply-To: <6.0.0.22.1.20060926141141.01b39e80@gemini.msu.montana.edu> Message-ID: <003001c6e1ad$4d6b0950$7701a80a@Ford> Just a general tech-note... if you have the clinical lab run your whole blood samples through their automated counters, make sure you inform them what species the sample is from. You mentioned that you are working with rats, so it should not be a problem with them getting an accurate WBC count. In some species (i.e. birds) the RBCs are nucleated and will not completely lyse with standard lysing reagents and therefore the RBCs could be erroneously counted as WBCs giving a elevated count. Regardless, it is important that your hematology department knows the species of the sample that you are asking them to run through their analyzer. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, September 26, 2006 3:22 PM To: Poteete, Jacquie A.; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] WBC counts We purchase hemocytometers from Fisher (Healthcare catalog) or VWR. We still use them for lung lavage/washings from mice. AND we do cell count aka differentials on Diff Quik (if you can get it without difficulty) stained murine lung lavage/cytospins. If you have to do a differential on animal blood smears, buffy coats, Wright Giemsa stain is superb for this purpose, Harleco brand. For a WBC count, contact a clinical laboratory to run the WBC counts on their automated counters, much more accurate and worth the cost. It was painful to work with the RBC and WBC pipettes - HOORAY for modern technology and instrumentation. At 01:38 PM 9/26/2006, you wrote: >Good luck finding a hemacytometer and/or any calibrated pipettes. Ours >ended up in the display of "antique instruments and equipment". That's >the best place for them (speaking as an old dinosaur who actually >learned how to use them). Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amanda.Garcia <@t> TriadHospitals.com Tue Sep 26 16:05:14 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Tue Sep 26 16:05:11 2006 Subject: [Histonet] Leasing histology equipment Message-ID: <8B63039C9DF4554C8FDBF31235F0E148020D349F@CPRTEVS02.triadhospitals.net> Can anyone direct me to a company that will lease histology equipment. Thanks in advance Amanda > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From AnthonyH <@t> chw.edu.au Tue Sep 26 17:56:27 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 26 17:57:55 2006 Subject: [Histonet] Fatty unfixed tissues? Message-ID: The best process I've found is to gently squeeze out the excess wax, place back into the cassette and return to 10% formalin. Reprocessing seems to fix the problem. Reference below: While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. I have often had recourse to use this method and have found it to dramatically improve the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson (2003) Histologic 36(1):21-22). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, 26 September 2006 10:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty unfixed tissues? Hi Everyone, Just wondering if anyone has any neat ideas about what to do with fatty tissue that comes out of the processor not processed. We have done the "squeeze" technique and then re soak in paraffin. That helps but any other ideas? It's one of those pathologists who has to put everything in the day it arrives and is not a thin slicer. Thanks in advance Sheila _________________________________________________________________ Don't waste time standing in line-try shopping online. Visit Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Sep 26 17:59:20 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 26 18:00:54 2006 Subject: [Histonet] Tissue in Paraffin Message-ID: Since the tissue is already processed to wax, you can safely remove the cassettes from the wax and let them harden to room temp. Then to embed, place them in molten wax for 30 min or so to remelt the tissue ready for embedding. The tissue is already infiltrated with wax so no harm can come to them. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Tuesday, 26 September 2006 10:21 PM To: Histonet (E-mail) Subject: [Histonet] Tissue in Paraffin Good Morning my fellow Histonetters, I was wondering how long you would leave tissue unembedded after pulling them out of paraffin. My processor is broke down. I would greatly appreciate the feedback. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Sep 26 18:06:50 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 26 18:08:14 2006 Subject: [Histonet] Decal for Immunos Message-ID: The following might be of use: Organic acids are slower decalcifiers and are usually gentler on tissues. Formic acid (5%) has been extensively used primarily as a fixative but has good decalcifying properties especially for bone marrow trephine biopsies. Picric acid is rarely used by itself but is usually used in combination with other acids or fixatives. Acetic acid tends to shrink tissues so is usually combined with other ingredients. Trichloroacetic acid (4%) is very energetic in its action but tends to deteriorate in solution. Foschini & Muzzi (1993) described the use of 7% citric acid for the decalcification of small calcifications such as psammoma bodies. These small calcifications can render tissues difficult to cut and often the tissue surrounding the concretion is shattered and disappears microscopically. Though this gentle decalcification is not appropriate for dense bones such as teeth and femurs, it is quite adequate for the small calcifications (no greater than 1-2mm) where overnight treatment is enough. EDTA solutions are also quite usefull Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Wilder Sent: Wednesday, 27 September 2006 2:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for Immunos Hi everyone, Does anyone know of a decal solution without HCL? It seems our RapidCal is interfering with some of the immuno stains. Thanks so much! Paula Wilder St. Joseph Medical Center Towson, MD 21204 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Sep 26 18:10:53 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 26 18:12:21 2006 Subject: [Histonet] off-topic re: our Tim Morken Message-ID: Oh no, Does this mean we will have Tim Morken groupies? Beware Tim - I believe that the Paparazzi may have their eyes on you. Well done Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, 27 September 2006 2:56 AM To: histonet Subject: Re: [Histonet] off-topic re: our Tim Morken In his textbook photo, Tim looks very professional, and handsome, of course. And I can attest that as far as 10th grade biology books go, this one is Great. It even has a section on Stem Cells in it. I find myself reading the darn thing for pleasure... Oh geeeez, am I officially becoming a biology nerd? Jan ----- Original Message ----- From: "Morken, Tim" To: "histonet" Sent: Monday, September 25, 2006 2:54 PM Subject: RE: [Histonet] off-topic re: our Tim Morken Hey, it was only about 4 years ago! Those who have been around awhile might remember a freelance writer posted on Histonet looking for a histotech to interview and to take pictures of in the lab. I volunteered and the rest is history. I even got a free copy of the book! It is "Biology" (high school) by Prentice-Hall publishers and has a dragonfly on the cover. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCID) Sent: Saturday, September 23, 2006 4:21 AM To: Jan Shivers; histonet Subject: RE: [Histonet] off-topic re: our Tim Morken Okay, inquiring minds and all that stuff......how old was the picture???? Sorry, Tim. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/22/2006 2:33 PM To: histonet Cc: Subject: [Histonet] off-topic re: our Tim Morken I was paging through my children's new 10th grade biology textbook, and whose photo do I see on a page, but our own Tim Morken! He was featured as an example of a Histotechnologist in the textbook's focus on biology careers. I shouted... "Hey, I 'know' this guy!" (Well,... not really know know... but you all understand what I mean.) Congratulations to Tim on getting his face in print, as well as his words! Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From laurie.reilly <@t> jcu.edu.au Tue Sep 26 18:32:45 2006 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Tue Sep 26 18:30:36 2006 Subject: [Histonet] control material In-Reply-To: <95C57EA9EF5C5A41AC7E075734B75259E42BD4@elht-exch2.xelht.nh s.uk> Message-ID: <5.2.0.9.0.20060927092357.037f6eb8@mail.jcu.edu.au> Jean and Histonetters, Dogs are prone to skin lesions caused by Mycobacterium species which stain well with Ziehl-Neelson. If this is considered a valid control, your nearest Veterinary Path lab could probably supply you with a block. Regards, Laurie. At 11:49 AM 26/09/2006 +0100, Gillson Jean (ELHT) Pathology wrote: Hi all, we are very desperate for control material for tuberculosis (ZN stain, toludine blue) and CEA immunocychemistry. If any one can help by providing us with material or directing me to a commercial supplier then I would be extremely grateful. Thankyou Jean _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 References 1. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immrstambo <@t> hotmail.com Tue Sep 26 18:35:39 2006 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Tue Sep 26 18:35:46 2006 Subject: [Histonet] Fatty unfixed tissues? In-Reply-To: Message-ID: We reprocess fatty or unfixed tissue. Simply melt block down, put in a new cassette and immerse in histoclear, xylene, formula 83 or whatever substitute you may be using for about 2 hours. Then just put it back in formalin and reprocess with the next batch of tissue. It will be delayed 1 day, but it generally cuts and stains much better. Good Luck! Christine Tambasco, HT (ASCP) St. Mary's Hospital at Amsterdam, New York 518-841-7287 ______________________________________________________________ From: "sheila adey" To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty unfixed tissues? Date: Tue, 26 Sep 2006 08:12:11 -0400 >Hi Everyone, > >Just wondering if anyone has any neat ideas about what to do with >fatty tissue that comes out of the processor not processed. We have >done the "squeeze" technique and then re soak in paraffin. That >helps but any other ideas? It's one of those pathologists who has to >put everything in the day it arrives and is not a thin slicer. > >Thanks in advance > >Sheila > >_________________________________________________________________ >Dont waste time standing in linetry shopping online. Visit >Sympatico / MSN Shopping today! http://shopping.sympatico.msn.ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Sep 26 19:40:15 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Sep 26 19:40:45 2006 Subject: [Histonet] Microwave processors Message-ID: A graduate from our Histotechology program is in the market for a microwave processor and has asked for my advice. I have no working knowledge of the microwave processors. Can anyone provide the pros and cons of the microwave processors on the market? What should she look for? Thank you for your help. Jennifer MacDonald Mt. San Antonio College Walnut, CA From bayoubelle311 <@t> gmail.com Tue Sep 26 19:59:41 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Tue Sep 26 19:59:46 2006 Subject: [Histonet] Re: WBC Counts Message-ID: <398f02c20609261759g24397ec0j907295acf2391df8@mail.gmail.com> Thanks all... I wish I could hand it over to Hem, unfortunately we don't have a Hem dept. I am working through it with the 100 cell count differential. We'll see how it goes, the staining was not done very well either, so we may just have to figure out something else. Thanks for the suggestions tho :) From Stephen.Eyres <@t> sanofi-aventis.com Wed Sep 27 02:43:08 2006 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Wed Sep 27 02:46:17 2006 Subject: [Histonet] Honey as a formalin substitute Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D4E2447@ALPW31.f2.enterprise> And don't forget the reduced costs of waste disposal -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vinnie Della Speranza Sent: Tuesday, September 26, 2006 4:15 PM To: Philip.Bryant@bromor-tr.wales.nhs.uk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Honey as a formalin substitute Hi Phil, it was good to see and speak with you in Phoenix. now about that Coca Cola, do you know if tissues decal'd in this solution are suitable for immunohistochemistry ?? we never seem to have a shortage of Diet Coke here in the lab, might be a good substitute in a pinch ! 8>) Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Philip Bryant" 09/26/06 >>> 08:02AM >>> Now that all the jokes and puns about honey have been exhausted, I thought that I would thank everyone for their interest and comments, positive or otherwise, regarding my publication. When the paper was written, it was intended as a basic, wacky and off the wall idea. It certainly proved to be that and I'm grateful for the publicity it generated. I would also be pleased to hear any other comments or results of any independent research that may come as a result. Apparently, the supermarkets in the UK are finding it hard to compete with the demand for honey and fresh liver!!!! By the way, do you know about the decalcifying powers of Coca Cola.......?? Happy researching! Phil Phil Bryant PhD CSci FIBMS Cellular Pathology Team Manager Department of Pathology Princess of Wales Hospital Bridgend Mid Glamorgan Wales, UK CF31 1RQ Phone : 01656 752320 E-mail : philip.bryant@bromor-tr.wales.nhs.uk Cymraeg:- Mae'r neges hon yn gyfrinachol.Os nad chi yw'r derbynnydd y bwriedid y neges ar ei gyfer, byddwch mor garedig ? rhoi gwybod i'r anfonydd yn ddi-oed. Dylid ystyried un rhywd datganiadau neu sylwadau a wneir uchod yn rhai personol,ac nid o angen rhaid yn rhai o eiddo Ymddiriedolaeth GIG Bro Morgannwg, nac unrhyw ran gyfansoddol ohoni na chorff cysylltiedig. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Bro Morgannwg roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau'r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Bro Morgannwg ar www.bromor-tr.wales.nhs.uk English:- This message is confidential. If you are not the intended recipient of the message then please notify the sender immediately. Any of the statements or comments made above should be regarded as personal and not necessarily those of Bro Morgannwg NHS Trust, any constituent part or connected body. Please be aware that, under the terms of the Freedom of Information Act 2000, Bro Morgannwg NHS Trust may be required to make public the content of any emails or correspondence received. For further information on Freedom of Information, please refer to the Bro Morgannwg NHS Trust website at www.bromor-tr.wales.nhs.uk. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Sep 27 04:50:56 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Wed Sep 27 04:58:15 2006 Subject: [Histonet] Microwave processors Message-ID: It depends on what they need it for. For a few samples I had success with the Milestone TT/Mega but if you plan to do a lot of processing the continuous-load, walk-away features of the Sakura Xpress are great. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 26, 2006 8:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processors A graduate from our Histotechology program is in the market for a microwave processor and has asked for my advice. I have no working knowledge of the microwave processors. Can anyone provide the pros and cons of the microwave processors on the market? What should she look for? Thank you for your help. Jennifer MacDonald Mt. San Antonio College Walnut, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hodges420 <@t> msn.com Wed Sep 27 07:23:34 2006 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Wed Sep 27 07:23:49 2006 Subject: [Histonet] EGFR cost to patient Message-ID: Good morning all, Can anyone advise me as to how you are charging for EGFR to help recover the cost of the antibody? Thanks Tere Hodges St Mary's Hospital Tucson, Az From cpomajzl <@t> cpllabs.com Wed Sep 27 07:41:22 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Wed Sep 27 07:34:14 2006 Subject: [Histonet] First Ever Brain Atlas Completed Message-ID: <002b01c6e232$4304ad60$26fca8c0@CSP> The first ever 3-D brain atlas is now complete. The Allen Institute for Brain Scienece, with the help of $100 million from Microsoft co-founder Paul Allen, has put together the online atlas that contains 600 terabytes of data, equivalent to 20,000 iPods according to the article. ARTICLE: http://news.nationalgeographic.com/news/2006/09/060926-brain-atlas.html WEBSITE: http://www.brain-map.org Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. From dellav <@t> musc.edu Wed Sep 27 08:27:43 2006 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Wed Sep 27 08:28:14 2006 Subject: [Histonet] Fatty unfixed tissues? Message-ID: a neat technique that does NOT require the removal of paraffin appeared in = HistoLogic May 2003 authored by Mickie Johnson. you can find it at = www.sakuraus.com Vinnie >>> "sheila adey" 09/26/06 08:12AM >>> Hi Everyone, Just wondering if anyone has any neat ideas about what to do with fatty=20 tissue that comes out of the processor not processed. We have done the=20 "squeeze" technique and then re soak in paraffin. That helps but any = other=20 ideas? It's one of those pathologists who has to put everything in the = day=20 it arrives and is not a thin slicer. Thanks in advance Sheila _________________________________________________________________ Don=92t waste time standing in line=97try shopping online. Visit Sympatico = / MSN=20 Shopping today! http://shopping.sympatico.msn.ca=20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu=20 http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jlinda <@t> ces.clemson.edu Wed Sep 27 08:42:58 2006 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Wed Sep 27 08:37:52 2006 Subject: [Histonet] coverslipping Message-ID: <5.2.1.1.2.20060927093144.01fa65e0@mailhost.ces.clemson.edu> John, Even though Rebecca gave a wonderful description of coverslipping, this is a technique that benefits from a hands-on demonstration. I would recommend that you call the Phelps County Hospital, ask for the laboratory and see if they have a histology department. Ask the histologist if she/he would be so kind as to teach you how to coverslip. If they don't have a histology department, ask for hematology. This was the hardest manual technique for me to master as a novice histologist and I cannot imagine learning how to do this technique by reading about it. There may also be a private pathology lab in Rolla that you can access (my hubby went to UMR and my first child was born at the hospital) but I have been gone from there for many years and I am sure things have changed. Good Luck and, remember, NO air bubbles allowed:-) Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From rjbuesa <@t> yahoo.com Wed Sep 27 11:24:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 27 11:24:57 2006 Subject: [Histonet] Tissue in Paraffin In-Reply-To: Message-ID: <20060927162452.55852.qmail@web61211.mail.yahoo.com> I prefer to place the cassettes in a low carboard box of adequate size, filled with melted paraffin, and to make like a gigantic "common" paraffin block for all, that will be melted as later on to prepare the final individual tissue blocks. Ren? J. Tony Henwood wrote: Since the tissue is already processed to wax, you can safely remove the cassettes from the wax and let them harden to room temp. Then to embed, place them in molten wax for 30 min or so to remelt the tissue ready for embedding. The tissue is already infiltrated with wax so no harm can come to them. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Tuesday, 26 September 2006 10:21 PM To: Histonet (E-mail) Subject: [Histonet] Tissue in Paraffin Good Morning my fellow Histonetters, I was wondering how long you would leave tissue unembedded after pulling them out of paraffin. My processor is broke down. I would greatly appreciate the feedback. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From Karen.Heckford <@t> CHW.edu Wed Sep 27 12:49:22 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Sep 27 12:49:46 2006 Subject: [Histonet] Thanks Everyone! Message-ID: Thank you to everyone that responded about leaving tissue unembedded. We are finally getting a new processor !! I am so excited. I cannot say enough about my local histotechs (UCSF, Marin Medical Laboratory, Lee Histology Lab and last but not least Histo Tec. Laboratory) coming to my rescue and bailing me out so we did not lose much turn around time. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From fawn <@t> cs.cmu.edu Wed Sep 27 13:03:02 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Wed Sep 27 13:03:07 2006 Subject: [Histonet] help with immuno's Message-ID: <451ABCD6.8090907@cs.cmu.edu> Okay everybody, I know I sent out an e-mail about helping with immuno's on monday, but now I have some more specific information. If anyone could help me with any procedures using the following primary and secondary antibodies that would be great. The doctor would like to use RunX2 Goat Polyclonal as the primary antibody and CY3 as the secondary. Or Rabbit Polyclonal primary with an Alk phos secondary. Thanks in advance Fawn From david.kinsley <@t> spcorp.com Wed Sep 27 13:05:25 2006 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Wed Sep 27 13:05:37 2006 Subject: [Histonet] Zymed Mozaic IHC stainer Message-ID: Hi, We recently purchased a Zymed Mozaic automated immuno stainer and seem to have a carry over issue. I have tried 2 test runs with a CD68 antibody on 10 human tonsil slides. Slides 1-9 are stained with the CD68 antibody and slide #10 is PBS for a negative control. In 2 separate runs my negative control has weak positive staining. When I tried the same protocol by hand, my negative control slide is clean. I feel that there is some carry over of primary antibody that is either sticking to the outside of the pipette, or remaining in the tubing during the pipetting process. Has anyone had this problem before? How did you solve it? If anyone has any tips for working with this instrument they are appreciated. thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From jblaine <@t> bio-search.com Wed Sep 27 13:12:35 2006 From: jblaine <@t> bio-search.com (Jason Blaine) Date: Wed Sep 27 13:11:25 2006 Subject: [Histonet] Histotechnician Position announcement - NIH / NEI Message-ID: <200609271311984.SM01888@BIOSEARCH> BioSearch, a leading provider of research support service professionals to the Federal Government, is seeking an experienced Histotechnician to provide service to the NIH's National Eye Institute. This position is a fulltime temporary contract assignment. Responsibilities: Cut 20 - 30 blocks of methacrylate per day Preparation of Cytospin slides and staining - 2 cases per week Act as back-up to primary personnel for Special Stains Here are the details: Days: Monday - Friday (no holidays) Hours: 7:30AM - 4:00PM (flexible within an hour either direction) Location: Bethesda, MD 20892 Duration: 90+ days Compensation: competitive Requirements: Previous experience with the eye is highly desired but not required. Qualified candidate please send resume/CV to jblaine@bio-search.com From laurie.colbert <@t> huntingtonhospital.com Wed Sep 27 14:19:43 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Sep 27 14:19:49 2006 Subject: [Histonet] Block Trays Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653D85@EXCHANGE1.huntingtonhospital.com> Does anyone know if there are trays available (and if so, where) that will hold your blocks after cutting. The tray slants forward, so that your blocks will be in the right order for filing later. I am looking for something that is relatively small - one that will fit in at the cutting area. Laurie Colbert From akbitting <@t> geisinger.edu Wed Sep 27 14:29:16 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Sep 27 14:29:35 2006 Subject: [Histonet] neon bulb array help Message-ID: <451A98CC020000C900000243@GHSGWIANW5V.GEISINGER.EDU> Hi Histonetters! Anyone out there trying to make their Neon Bulb Array so that they can test theor microwaves energy pattern is going to find that Radio Shack doesn't carry that size bulb anymore and hasn't replaced it with another item. Sooo, because the Microwave Toolbook doesn't really give a description of that bulb, just the Radio Shack part number, I'm at a loss. Does anyone know what the specs are on that neon bulb or know an identical part number. I'm sure Lowes or Home Depot must sell them, but I don't even know what to ask them for. Help! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From pruegg <@t> ihctech.net Wed Sep 27 14:30:10 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Sep 27 14:30:23 2006 Subject: [Histonet] HT exam Message-ID: <00fa01c6e26b$59e07c60$6601a8c0@Patsy> Can anyone field this question? Please reply directly to Pat Barnes at queensd54@yahoo.com My student and I have been searching for information for some time on the explanation of coagulant -non coaglant fixatives. Sheehan and Carson have opposite definitions and all of our other material is ambiguous. Can you help me shed some light on what could be the best answer if it is on his test Friday? All opinions are welcome. Thanks. Pat Life is short. Enjoy daily. Shalom. Patsy Ruegg IHCtech 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net From portera <@t> msu.edu Wed Sep 27 14:32:07 2006 From: portera <@t> msu.edu (Amy Porter) Date: Wed Sep 27 14:31:06 2006 Subject: [Histonet] Block Trays References: <0BE6ADFAE4E7E04496BF21ABD346628005653D85@EXCHANGE1.huntingtonhospital.com> Message-ID: <003e01c6e26b$9ea30660$8e7a0923@HistoJJ> Try MarketLab, we have some that are disposable and interchangeable that sit on an acrylic stand. www.marketlabinc.com ----- Original Message ----- From: "Laurie Colbert" To: Sent: Wednesday, September 27, 2006 3:19 PM Subject: [Histonet] Block Trays Does anyone know if there are trays available (and if so, where) that will hold your blocks after cutting. The tray slants forward, so that your blocks will be in the right order for filing later. I am looking for something that is relatively small - one that will fit in at the cutting area. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Wed Sep 27 14:38:29 2006 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Sep 27 14:36:20 2006 Subject: [Histonet] Block Trays In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628005653D85@EXCHANGE1.huntingtonhospital.com> References: <0BE6ADFAE4E7E04496BF21ABD346628005653D85@EXCHANGE1.huntingtonhospital.com> Message-ID: <451AD335.80706@rci.rutgers.edu> How about these, from Marketlab? http://www.marketlabinc.com/products/details/478 http://www.marketlabinc.com/products/details/171 Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 Laurie Colbert wrote: >Does anyone know if there are trays available (and if so, where) that will hold your blocks after cutting. The tray slants forward, so that your blocks will be in the right order for filing later. I am looking for something that is relatively small - one that will fit in at the cutting area. > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Wed Sep 27 14:52:24 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 27 14:52:31 2006 Subject: [Histonet] neon bulb array help In-Reply-To: <451A98CC020000C900000243@GHSGWIANW5V.GEISINGER.EDU> Message-ID: <20060927195224.92436.qmail@web61219.mail.yahoo.com> Contact Ted Pella, they sell those bulbs (I bought mine from them). Ren? J. Angela Bitting wrote: Hi Histonetters! Anyone out there trying to make their Neon Bulb Array so that they can test theor microwaves energy pattern is going to find that Radio Shack doesn't carry that size bulb anymore and hasn't replaced it with another item. Sooo, because the Microwave Toolbook doesn't really give a description of that bulb, just the Radio Shack part number, I'm at a loss. Does anyone know what the specs are on that neon bulb or know an identical part number. I'm sure Lowes or Home Depot must sell them, but I don't even know what to ask them for. Help! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From rjbuesa <@t> yahoo.com Wed Sep 27 15:04:00 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 27 15:04:07 2006 Subject: [Histonet] neon bulb array help In-Reply-To: <20060927195224.92436.qmail@web61219.mail.yahoo.com> Message-ID: <20060927200400.25720.qmail@web61213.mail.yahoo.com> Angela: Another important thing though. With those bulbs you will be able to determine "hot" and "cold" spots in you EMPTY microwave oven, but the moment you introduce ANY water load or specimen in it, that determination so painstakingly obtained IS USELESS since that same water load or the specimen will completely change the way the heat is distributed in your microwave oven. Once I was able to find that out I stopped wasting my time with bulb arrays. Hope this will help you!. Ren? J. Rene J Buesa wrote: Contact Ted Pella, they sell those bulbs (I bought mine from them). Ren? J. Angela Bitting wrote: Hi Histonetters! Anyone out there trying to make their Neon Bulb Array so that they can test theor microwaves energy pattern is going to find that Radio Shack doesn't carry that size bulb anymore and hasn't replaced it with another item. Sooo, because the Microwave Toolbook doesn't really give a description of that bulb, just the Radio Shack part number, I'm at a loss. Does anyone know what the specs are on that neon bulb or know an identical part number. I'm sure Lowes or Home Depot must sell them, but I don't even know what to ask them for. Help! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From Gloria.A.Okland <@t> questdiagnostics.com Wed Sep 27 15:49:06 2006 From: Gloria.A.Okland <@t> questdiagnostics.com (Okland, Gloria A) Date: Wed Sep 27 15:49:29 2006 Subject: [Histonet] Giemsa stain for Malaria Message-ID: Hi Ya'll, My pathologist is wanting the histo lab to use automated Giemsa stain for the thick EDTA slides for Malaria. Our first test worked great, but now all the cells are washing off. Does anyone have a procedure for this? Thanks Gloria ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From pmcardle <@t> ebsciences.com Wed Sep 27 15:51:08 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed Sep 27 15:51:18 2006 Subject: [Histonet] neon bulb array help In-Reply-To: <451A98CC020000C900000243@GHSGWIANW5V.GEISINGER.EDU> References: <451A98CC020000C900000243@GHSGWIANW5V.GEISINGER.EDU> Message-ID: <451AE43C.3080400@ebsciences.com> Hi, and another "vendor response" if you'll forgive me: I don't believe that specs matter much for the neon bulbs in this application, the reason being that the microwaves are directly exciting the gas contained in the bulb; since it's not "electrical" per se, specs like voltage or wattage shouldn't matter. Do a google or yahoo search and you'll find lots of suppliers; go for the cheapest, and you DON'T need the type with a built-in resistor; the ones we used in the past were about 1/4" long and we clipped off the leads as short as possible (our design encased the bulbs entirely, so we didn't need the leads to hold the bulbs in place). Although we don't sell them, you can buy a pre-made neon bulb array from other sources, including Electron Microscopy Sciences (www.emsdiasum.com). It's apparent from your query that you're trying to quantify and document your microwave's performance, and we applaud this effort. However, you should be aware of limitations of what the array shows. At EBS we've found neon bulb arrays of limited practical usefulness for the following reason: any microwave's field changes as soon as anything is placed into the chamber. That said, the very fact that you're placing the bulb array into the chamber alters the microwave energy field. (Isn't that the "Westinghouse Effect:" the act of observation changes what you're trying to observe?) It is possible to re-test as you place different equipment into the microwave, for example, a Coplin jar, a 100mL container, etc., but the array also only shows what's reaching the chamber floor, not what's being absorbed by the equipment. It should be stressed that the array can reveal a major hotspot or cool spot, or show that your mode stirrer may not be working, and this has value since these are major defects that should be addressed immediately. Hope this helps! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com Angela Bitting wrote: > Hi Histonetters! > Anyone out there trying to make their Neon Bulb Array so that they can > test theor microwaves energy pattern is going to find that Radio Shack > doesn't carry that size bulb anymore and hasn't replaced it with another > item. > > Sooo, because the Microwave Toolbook doesn't really give a description > of that bulb, just the Radio Shack part number, I'm at a loss. > > Does anyone know what the specs are on that neon bulb or know an > identical part number. I'm sure Lowes or Home Depot must sell them, but > I don't even know what to ask them for. > > Help! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Sep 27 16:02:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 27 16:03:03 2006 Subject: [Histonet] neon bulb array help In-Reply-To: <451AE43C.3080400@ebsciences.com> Message-ID: <20060927210254.38217.qmail@web61218.mail.yahoo.com> No, that is Heisenberg's incertitude / uncertainty principle. Ren? J. Phil McArdle wrote: Hi, and another "vendor response" if you'll forgive me: I don't believe that specs matter much for the neon bulbs in this application, the reason being that the microwaves are directly exciting the gas contained in the bulb; since it's not "electrical" per se, specs like voltage or wattage shouldn't matter. Do a google or yahoo search and you'll find lots of suppliers; go for the cheapest, and you DON'T need the type with a built-in resistor; the ones we used in the past were about 1/4" long and we clipped off the leads as short as possible (our design encased the bulbs entirely, so we didn't need the leads to hold the bulbs in place). Although we don't sell them, you can buy a pre-made neon bulb array from other sources, including Electron Microscopy Sciences (www.emsdiasum.com). It's apparent from your query that you're trying to quantify and document your microwave's performance, and we applaud this effort. However, you should be aware of limitations of what the array shows. At EBS we've found neon bulb arrays of limited practical usefulness for the following reason: any microwave's field changes as soon as anything is placed into the chamber. That said, the very fact that you're placing the bulb array into the chamber alters the microwave energy field. (Isn't that the "Westinghouse Effect:" the act of observation changes what you're trying to observe?) It is possible to re-test as you place different equipment into the microwave, for example, a Coplin jar, a 100mL container, etc., but the array also only shows what's reaching the chamber floor, not what's being absorbed by the equipment. It should be stressed that the array can reveal a major hotspot or cool spot, or show that your mode stirrer may not be working, and this has value since these are major defects that should be addressed immediately. Hope this helps! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com Angela Bitting wrote: > Hi Histonetters! > Anyone out there trying to make their Neon Bulb Array so that they can > test theor microwaves energy pattern is going to find that Radio Shack > doesn't carry that size bulb anymore and hasn't replaced it with another > item. > > Sooo, because the Microwave Toolbook doesn't really give a description > of that bulb, just the Radio Shack part number, I'm at a loss. > > Does anyone know what the specs are on that neon bulb or know an > identical part number. I'm sure Lowes or Home Depot must sell them, but > I don't even know what to ask them for. > > Help! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From muddymoo <@t> gmail.com Wed Sep 27 21:51:01 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Wed Sep 27 21:51:07 2006 Subject: [Histonet] cassette writers Message-ID: Anyone got any recomendation for cassette writers? Need to go down that route by the end of the year but don't really know what is on offer at the moment and what is good or bad! All input appreciated. Cheers Alan Bishop Charge Scientist Histopathology Medlab Central Palmerston North New Zealand From Shirley_PHUA <@t> hsa.gov.sg Wed Sep 27 23:48:49 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed Sep 27 23:49:04 2006 Subject: [Histonet] Shirley is away on medical leave : 28 September 2006 (Thursday) Message-ID: I will be out of the office from 28-09-2006 to 29-09-2006. I will return on 29 September 2006 (Friday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From wulan <@t> med.kobe-u.ac.jp Thu Sep 28 03:03:27 2006 From: wulan <@t> med.kobe-u.ac.jp (Wulan Anggrahini) Date: Thu Sep 28 01:03:11 2006 Subject: [Histonet] VCAM-1, ICAM-1, P-selectin antibody Message-ID: <000f01c6e2d4$942a92c0$0ec8a8c0@your56ab121f6e> Hello... I need some recomendation to choose antibody for VCAM-1, ICAM-1 and P-selectin detection on mouse vascular tissue either processed as frozen or paraffin embedded section. I found so many available products and I have difficulties in choosing. If anyody have experience, please give me info. Thanks, Wulan From louise.renton <@t> gmail.com Thu Sep 28 02:36:37 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Sep 28 02:36:45 2006 Subject: [Histonet] observer's paradox Message-ID: Rene, phillip et al.... Schroedinger's cat, Heisenberg's uncertainty principle and the Westinghouse effect are all versions of the same thing, namely that the observation or measurement itself affects an outcome, so that it can never be known what the outcome would have been if it were not observed Misuse of Heisenberg's principle: http://www.slate.com/?id=2062844 On 9/27/06, Rene J Buesa wrote: > No, that is Heisenberg's incertitude / uncertainty principle. > Ren? J. > > Phil McArdle wrote: > Hi, and another "vendor response" if you'll forgive me: > > I don't believe that specs matter much for the neon bulbs in this > application, the reason being that the microwaves are directly exciting > the gas contained in the bulb; since it's not "electrical" per se, specs > like voltage or wattage shouldn't matter. Do a google or yahoo search > and you'll find lots of suppliers; go for the cheapest, and you DON'T > need the type with a built-in resistor; the ones we used in the past > were about 1/4" long and we clipped off the leads as short as possible > (our design encased the bulbs entirely, so we didn't need the leads to > hold the bulbs in place). > > Although we don't sell them, you can buy a pre-made neon bulb array from > other sources, including Electron Microscopy Sciences (www.emsdiasum.com). > > It's apparent from your query that you're trying to quantify and > document your microwave's performance, and we applaud this effort. > However, you should be aware of limitations of what the array shows. At > EBS we've found neon bulb arrays of limited practical usefulness for the > following reason: any microwave's field changes as soon as anything is > placed into the chamber. That said, the very fact that you're placing > the bulb array into the chamber alters the microwave energy field. > (Isn't that the "Westinghouse Effect:" the act of observation changes > what you're trying to observe?) It is possible to re-test as you place > different equipment into the microwave, for example, a Coplin jar, a > 100mL container, etc., but the array also only shows what's reaching the > chamber floor, not what's being absorbed by the equipment. It should be > stressed that the array can reveal a major hotspot or cool spot, or show > that your mode stirrer may not be working, and this has value since > these are major defects that should be addressed immediately. > > Hope this helps! > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > Energy Beam Sciences, Inc. > Tel: 800.992.9037 x 341 > Fax: 860.653.0422 > PMcardle@ebsciences.com > www.ebsciences.com > > > Angela Bitting wrote: > > Hi Histonetters! > > Anyone out there trying to make their Neon Bulb Array so that they can > > test theor microwaves energy pattern is going to find that Radio Shack > > doesn't carry that size bulb anymore and hasn't replaced it with another > > item. > > > > Sooo, because the Microwave Toolbook doesn't really give a description > > of that bulb, just the Radio Shack part number, I'm at a loss. > > > > Does anyone know what the specs are on that neon bulb or know an > > identical part number. I'm sure Lowes or Home Depot must sell them, but > > I don't even know what to ask them for. > > > > Help! > > > > Angela Bitting, HT(ASCP) > > Technical Specialist, Histology > > Geisinger Medical Center > > 100 N Academy Ave. MC 23-00 > > Danville, PA 17822 > > phone 570-214-9634 > > fax 570-271-5916 > > > > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your email and more, right on the new Yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lpwenk <@t> sbcglobal.net Thu Sep 28 05:13:19 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Sep 28 05:13:52 2006 Subject: [Histonet] HT exam In-Reply-To: <00fa01c6e26b$59e07c60$6601a8c0@Patsy> Message-ID: <000901c6e2e6$b904c1d0$97f12d4b@HPPav2> Oh, boy. One of my least favorite things to try to teach. I'm sure I'll raise a few hackles with my view on this. (Going to be long, so skip if not interested.) Coagulative and non-coagulative fixation concept goes back decades and decades, maybe even a century or so. My feeling is, it was more an observation of what the tissue/proteins "physically" seemed to be doing when placed in a chemical, rather than the actual chemical bonding that we now talk about. I demonstrate it to my students using raw egg whites. I get a bunch of lidded jars, and put about 20 mL of 10% NBF, 20% formalin, zinc-formalin, alcoholic formalin, 100% alcohol, acetone, Bouin, DB (alcoholic Bouin), acetic acid. Then I add 1 mL of egg white into each container, and we watch what happens and how quickly. - In the 10% and 20% formalin, the egg white remains "loose" - even days later, I can still swirl it with a small wooden pick. - The egg hardens up immediately in the 100% alcohol, acetone, Bouin, DB. - The zinc-formalin, alcoholic formalin and the acetic acid harden up slower - in a few hours, and end up harder than the 10-20% formalin, but softer than the other chemicals. Therefore, based on a bunch of experiments like this, it was decided that the following reagents are non-coagulative for proteins: - formalin/formaldehyde in any percent - all aldehyde fixatives (gluteraldehyde, paraformaldehyde) - potassium dichromate - osmium tetroxide (doesn't bind to protein, only lipids, so it does not coagulate proteins) All other chemicals were considered coagulative for proteins (acetic acid is a little of both, coagulative for nuclei, non-coagulative for cytoplasm, so it depends which author you are reading, as to whether acids are coagulative or non-coagulative. I lump it with coagulative.). Now, if you have a mixture, say alcoholic-formalin, since the alcohol is considered coagulative, while the formalin is non-coagulative, then the mixture is still considered coagulative. If there is even just one chemical that is coagulative in a fixative mixture and all the rest are non-coagulative, then that mixture is still considered coagulative. The egg white does coagulate in alcholic-formalin, but just at a slower rate. So, for the HT/HTL exam, learn that aqeous solutions that contain only formalin, potassium dichromate and/or osmium are the only non-coagulative. If the fixative contains any other reagent, it is now considered coagulative. (Example - Orth contains only potassium dichromate, formaladehyde and water. Since both are non-coagulative, Orth is non-coagulative. Helly contains potassium dichromate, formaldehyde, mercuric chloride and water. Even though the potassium dichromate and formaldehyde are non-coagulative, because of mercuric chloride is coagulative, the entire Helly fixative is considered coagulative (and back when I used to do the egg white test using mercuric chloride fixatives, yes, the egg white did coagulate).) I still teach this concept to my students, as it is in the textbooks and on the HT/HTL exams. I tell them to memorize it for my fixative test and then study it again for the ASCP exam, and then they can forget it after that. I personally don't think it is very helpful information when we are dealing with amino acids being cross-linked by fixatives. After all, even though the egg white in the formalin never hardens, I know the egg white proteins are fixed. And I am more concerned about how the dyes will react now that I've cross-linked different proteins, rather than "how much did the tissue become hard". I, and I think most other program directors of HT/HTL programs, prefer to teach the additive/non-additive concept, which is also the the ASCP exams. Additive means the chemical becomes physically bound (linked) to something in the tissue. Formalin, which is negatively charged, become cross-linked to positively charged amino acids (of which there are a lot in cytoplasm and fewer in nuclei). The overall effect is that with more positively charged amino acids being bound to the formaldehyde molecule, the tissue is now slightly more negatively charged. On the other hand, zinc and mercuric salts, which are positively charged, will bind to negatively charged amino acids (of which there are fewer in cytoplasm and more in nuclei). So because the metal salts are binding to positive amino acids, the tissue has more negative amino acids still available(nothing bound to them), so the tissue is now slightly more positively charged. All of this helps to explain why tissues stain differently with H&E (charged dyes pick up differently), IHC (different cross-links at different amino acids) and look different (nuclear preservation, for example). If this is a little hard to see in your mind, imagine 6 positive charge and 6 negative charges. Net charge is zero. Now bind a negative aldehyde to two of the positive charges. What we have left is 4 positives and 6 negatives. Net charge is more negative. That's how the aldehydes work. Zinc and mercury are the opposite. Being positively charged, they will bind 2 negative charges, yielding 6 positive and 4 negatives, net = more positively charged. There are basically two families of non-additives: One are the "dehydrants" - any alcohol (ethanol, methanol, etc.), acetone, etc. They remove water from the tissue, from between the proteins. This causes the proteins to shrink together. Now a positive and a negative amino acid (that used to be separated from each other by the water in the tissue) are moved closer to each other. Now, these two amino acids can bind to each other. The "dehydrants" are NOT chemically bound to the tissue. Chemically, since a positive and a negative bind to each other, there is no real change in the overall charge of the tissue/protein. (Start with the 6 + and 6 -, bind one + and one -, end result is 5 + and 5 -, so net charge is still zero.) So those lab that have shortened formalin fixation and their tissues are mostly alchohol fixed (no change in charges), their stains are going to look different than if their tissue was completely formalin fixed (tissue more negatively charged if formalin fixed, no change in charge if primary fixative was alcohol). Acids are the other non-additive. They are sort of the opposite of the dehydrants. They break open the naturally occuring protein bonds that occur when one positive amino acid is close to a negative amino acid. When these bonds break, these charged amino acids are now free to bind to water molecules. The proteins now swell. But the acids are NOT chemically bound to the tissue or protein. However, if there is a mixture, we do NOT lump all the chemicals together and CANNOT say the mixture is either additive or non-additive. We say what each separate chemical is doing. Zenker then would be: mercuric chloride = additive; potassium dichromate = additive; acetic acid = non-additive. In both additive/non-additive and the coagulative/non-coagulative, the water and any buffering salts are NOT considered. So the easy way to remember all this: Non-coagulative = APDO = any aldehyde, potassium dichromate, osmium Coagulative = all the rest Mixture = if there is even one coagulative, then the mixture is coagulative. Non-additive = 3 A's = alcohols, acetone, acids Additive = all other chemicals Mixture = cannot be lumped into any category. Consider each chemical separately. Sorry for being long-winded. These are some hard concepts, and I've actually simplified it a lot. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, September 27, 2006 3:30 PM To: 'Histonet' Cc: queensd54@yahoo.com Subject: [Histonet] HT exam Can anyone field this question? Please reply directly to Pat Barnes at queensd54@yahoo.com My student and I have been searching for information for some time on the explanation of coagulant -non coaglant fixatives. Sheehan and Carson have opposite definitions and all of our other material is ambiguous. Can you help me shed some light on what could be the best answer if it is on his test Friday? All opinions are welcome. Thanks. Pat Life is short. Enjoy daily. Shalom. Patsy Ruegg IHCtech 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 28 07:28:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 28 07:28:14 2006 Subject: [Histonet] cassette writers In-Reply-To: Message-ID: <20060928122809.50642.qmail@web61219.mail.yahoo.com> Alan: Sakura Finetek has made the decision to cover the whole histology work flow with automated instruments and has also a cassette writer. If quality and reliability of a company is something to take into consideration, I would ask for a demo to evaluate if I were in your position! Ren? J. Alan Bishop wrote: Anyone got any recomendation for cassette writers? Need to go down that route by the end of the year but don't really know what is on offer at the moment and what is good or bad! All input appreciated. Cheers Alan Bishop Charge Scientist Histopathology Medlab Central Palmerston North New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Thu Sep 28 07:28:40 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 28 07:28:48 2006 Subject: [Histonet] cassette writers In-Reply-To: Message-ID: <20060928122840.70705.qmail@web61214.mail.yahoo.com> Alan: Sakura Finetek has made the decision to cover the whole histology work flow with automated instruments and has also a cassette writer. If quality and reliability of a company is something to take into consideration, I would ask for a demo to evaluate if I were in your position! Ren? J. Alan Bishop wrote: Anyone got any recomendation for cassette writers? Need to go down that route by the end of the year but don't really know what is on offer at the moment and what is good or bad! All input appreciated. Cheers Alan Bishop Charge Scientist Histopathology Medlab Central Palmerston North New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From tbraud <@t> holyredeemer.com Thu Sep 28 07:56:43 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Sep 28 07:58:12 2006 Subject: [Histonet] Antigen retrieval in the microwave Message-ID: Thanks for the clarification (which I promptly took to our safety guru, right after digging my pressure cooker out of the garbage) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Phil McArdle Sent: Tuesday, September 26, 2006 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Antigen retrieval in the microwave If you'll forgive a vendor for butting in... Regarding the microwave pressure cooker: Let's not confuse "microwave transparency" with what we typically associate with the concept of transparency: /optical/ transparency. They're not the same. Microwave transparency refers to an object's ability to allow passage of microwave energy without converting a significant portion to heat. While some optically transparent objects happen also to be microwave transparent, that's not always true; leaded crystal in a microwave would be a disaster. Conversely, opaque white Teflon isn't something we'd think of as "transparent," yet it IS "microwave transparent." EBS has just produced a "Microwave Companion for Histology," a 20-page booklet that seeks to put to rest just this kind of confusion, and it's free for the asking. It's purely informational, not looking to sell anything, with a view towards "advancing the art" rather than "shameless commerce." Please contact me directly and I'll be happy to send you one, or a few. Best regards, Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com Terri Braud wrote: > The trick will be to only use transparent containers, another CAPmania reg...my microwave pressure cooker just got 86'd. I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale. Terri > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supv. > Laboratory > Holy Redeemer Hospital > (215) 938-3689 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela > Bitting > Sent: Tuesday, September 26, 2006 2:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Antigen retrieval in the microwave > > > I want to make sure I'm, totally CAP compliant, soooooo here's my > question. > Has anyone gotten cited for heating their citrate buffer or EDTA (for > antigen retrieval) in an unvented microwave? > I'm getting the impression that CAP wants all microwaves vented even > though they throw out that blurb about "unnecessary if only > non-hazardous solutions are being heated, i.e. water, some biological > stains, paraffin sections". > > > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it is addressed. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail at the electronic address shown. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From Charles.Embrey <@t> carle.com Thu Sep 28 08:16:01 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Sep 28 08:16:12 2006 Subject: [Histonet] cassette writers Message-ID: <44780C571F28624DBB446DE55C4D733A1FE458@EXCHANGEBE1.carle.com> Alan, We tested three different units this year, Sakura, TBS and Thermo. The thermo unit was the only one that impressed us as to reliability. The other two jammed up quite a bit. Needless to say, my thermo unit is now on order. Charles Embrey, PA(ASCP),HT Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Bishop Sent: Wednesday, September 27, 2006 9:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette writers Anyone got any recomendation for cassette writers? Need to go down that route by the end of the year but don't really know what is on offer at the moment and what is good or bad! All input appreciated. Cheers Alan Bishop Charge Scientist Histopathology Medlab Central Palmerston North New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgdelaware <@t> comcast.net Thu Sep 28 08:49:37 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Thu Sep 28 08:49:42 2006 Subject: [Histonet] Histology Position Message-ID: <000d01c6e304$f0444c60$0711c847@D7XQNX91> We have a full time Histology position open in Dover, DE. For more details contact: Marian Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Dr. Dover, DE 19904 (302)-677-0000 From algranth <@t> u.arizona.edu Thu Sep 28 09:20:04 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Sep 28 09:20:16 2006 Subject: [Histonet] Tissue in Paraffin In-Reply-To: <20060927162452.55852.qmail@web61211.mail.yahoo.com> References: Message-ID: <4.3.2.7.2.20060928071857.00c67600@algranth.inbox.email.arizona.edu> We have done both of these things and have not noticed a difference. When the tissues were finally embedded and cut they were fine. Andi At 09:24 AM 9/27/2006 -0700, you wrote: >I prefer to place the cassettes in a low carboard box of adequate size, >filled with melted paraffin, and to make like a gigantic "common" paraffin >block for all, that will be melted as later on to prepare the final >individual tissue blocks. > Ren? J. > >Tony Henwood wrote: > Since the tissue is already processed to wax, you can safely remove the >cassettes from the wax and let them harden to room temp. Then to embed, >place them in molten wax for 30 min or so to remelt the tissue ready for >embedding. The tissue is already infiltrated with wax so no harm can >come to them. > >Regards > >Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) >Laboratory Manager & Senior Scientist >The Children's Hospital at Westmead, >Locked Bag 4001, Westmead, 2145, AUSTRALIA. >Tel: 612 9845 3306 >Fax: 612 9845 3318 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Heckford, Karen - SMMC-SF >Sent: Tuesday, 26 September 2006 10:21 PM >To: Histonet (E-mail) >Subject: [Histonet] Tissue in Paraffin > > >Good Morning my fellow Histonetters, I was wondering how long you would >leave tissue unembedded after pulling them out of paraffin. My >processor is broke down. I would greatly appreciate the feedback. >Cheers, >Karen Heckford HT (ASCP) CE >Lead Histology Technician >Histology/Pathololgy Department >St. Mary's Medical Center >450 Stanyan St. >San Francisco, Ca. 94117 >415-668-1000 ext. 6167 >Fax: 415-750-8123 >email: kheckfor@chw.edu > >********************************************************************** >This email and any files transmitted with it are confidential and intended >solely for the use of the individual or entity to whom they are addressed. >If you are not the intended recipient, please delete it and notify the sender. > >Views expressed in this message and any attachments are those of the >individual sender, and are not necessarily the views of The Children's >Hospital at Westmead > >This note also confirms that this email message has been >virus scanned and although no computer viruses were detected, the >Childrens Hospital at Westmead accepts no liability for any consequential >damage resulting from email containing computer viruses. >********************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small >Business. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From manningl <@t> inspection.gc.ca Thu Sep 28 09:21:54 2006 From: manningl <@t> inspection.gc.ca (Lisa Manning) Date: Thu Sep 28 09:22:20 2006 Subject: [Histonet] Re: effects of elevation on immunohistochemistry Message-ID: Good morning, Does anyone have any experience or knowledge about the effects of elevation on immunohistochemistry? Thanks kindly, Lisa Manning Lisa Manning R.T, B.Sc. Histopathology Biologist Canadian Food Inspection Agency National Centre for Foreign Animal Disease 1015 Arlington Avenue, Suite T2300 Winnipeg, Manitoba R3E 3M4 PH. (204) 789-2087 FAX (204)789-2038 email: manningl@inspection.gc.ca From victor <@t> pathology.washington.edu Thu Sep 28 09:35:08 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Sep 28 09:35:22 2006 Subject: [Histonet] cassette writers In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE458@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A1FE458@EXCHANGEBE1.carle.com> Message-ID: <451BDD9C.208@pathology.washington.edu> We have 3 thermo units with the oldest one purchased in 2000. They have been extremely reliable and can print 2D bar-codes. The only drawback to the current model is that to add the bar-code to the cassette requires about 40 seconds per cassette. This is unacceptable in a high volume facility. They are coming out with the next generation model at the end of the year or early 07 which has a new print head that will take approx. 5 seconds per cassette. We are drooling over the prospects. Victor Charles.Embrey wrote: > Alan, We tested three different units this year, Sakura, TBS and > Thermo. The thermo unit was the only one that impressed us as to > reliability. The other two jammed up quite a bit. Needless to say, my > thermo unit is now on order. > > Charles Embrey, PA(ASCP),HT > Carle Clinic, Illinois > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan > Bishop > Sent: Wednesday, September 27, 2006 9:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette writers > > Anyone got any recomendation for cassette writers? Need to go down that > route by the end of the year but don't really know what is on offer at > the > moment and what is good or bad! > > All input appreciated. > > Cheers > > Alan Bishop > Charge Scientist > Histopathology > Medlab Central > Palmerston North > New Zealand > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From GDawson <@t> dynacaremilwaukee.com Thu Sep 28 09:37:00 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Sep 28 09:37:09 2006 Subject: [Histonet] Re: effects of elevation on immunohistochemistry Message-ID: I don't know much about the possible effects of elevation on immunohistochemistry but I see another section being added to the CAP inspection checklist now. Thanks alot Lisa. Just kidding btw. The obvious Biggie would be Heat Induced Epitope Retrieval (HIER). I would suggest a pressure cooker in higher elevations to overcome this one. My usual water bath HIER wouldn't be as effective with the lower boiling points seen in higher elevations. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lisa Manning Sent: Thursday, September 28, 2006 8:22 AM To: histonet@lists.utsouthwestern.edu; Sundi Readlinger Subject: [Histonet] Re: effects of elevation on immunohistochemistry Good morning, Does anyone have any experience or knowledge about the effects of elevation on immunohistochemistry? Thanks kindly, Lisa Manning Lisa Manning R.T, B.Sc. Histopathology Biologist Canadian Food Inspection Agency National Centre for Foreign Animal Disease 1015 Arlington Avenue, Suite T2300 Winnipeg, Manitoba R3E 3M4 PH. (204) 789-2087 FAX (204)789-2038 email: manningl@inspection.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Sep 28 09:43:10 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Sep 28 09:43:15 2006 Subject: [Histonet] Explanatory Formula Message-ID: I once worked for a veterinarian who, shall we say, liked to embellish the facts. He never lied -- he embellished and he was very entertaining. We put this into formula style: "Kelliher Factor: The square root of the truth times ten to the minus six". This has very little relevance to today's discussions but I had a minute and I'm trying to work up a subject for FRIDAY HOUR OF FUMING. Any takers? Are we all that darned HAPPY??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Thu Sep 28 09:44:59 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 28 09:45:10 2006 Subject: [Histonet] Re: effects of elevation on immunohistochemistry In-Reply-To: Message-ID: <20060928144459.33480.qmail@web61218.mail.yahoo.com> Lisa: This is a Physics issue! At higher elevation atmosferic pressure is lower and the water boils at less than 100?C so any HIER done in an "open" container, like heating in the MW oven or using a steamer or a water bath, will be affected because 100?C cannot be reached. HIER should be done in a pressurized environment (like a pressure cooker). There was a paper published in the Jorunal of Histotechnology not too long ago (I do not have the exact reference) dealing with Her-2 Neu tests at high elevation. Any step requiring a certain temperature in an open container, will be affected. Other steps temperature independednt will not be affected. Hope this will help you! Ren? J. Lisa Manning wrote: Good morning, Does anyone have any experience or knowledge about the effects of elevation on immunohistochemistry? Thanks kindly, Lisa Manning Lisa Manning R.T, B.Sc. Histopathology Biologist Canadian Food Inspection Agency National Centre for Foreign Animal Disease 1015 Arlington Avenue, Suite T2300 Winnipeg, Manitoba R3E 3M4 PH. (204) 789-2087 FAX (204)789-2038 email: manningl@inspection.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From Jerry <@t> ralambusa.com Thu Sep 28 09:45:41 2006 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Thu Sep 28 09:45:55 2006 Subject: [Histonet] 5. Block Trays (Laurie Colbert) Message-ID: <3855F92002259948A66A8CA2D16E3A4F01779A@server.ralambusa.com> Lori, Raymond A. Lamb has a Cassette tray/box that can take approximately 200 processing cassettes in 5 rows. Please see http://www.ralamb.net/product_info.php?products_id=85 Jerry Helisek? VP - North America 5409 Lumley Road, Unit #102 Durham, North Carolina 27703 Phone: 919-957-1964 Fax: 919-957-1972 Cell: 919-264-7964 jerry@ralambusa.com www.ralamb.com The information contained in this e-mail message may be privileged, confidential and protected from disclosure. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you think that you have received this e-mail message in error please e-mail the sender and delete the message. Thankyou. From victor <@t> pathology.washington.edu Thu Sep 28 10:05:05 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Sep 28 10:05:17 2006 Subject: [Histonet] Histology Position in Seattle Message-ID: <451BE4A1.1060301@pathology.washington.edu> There is a FT position available with the University of Washington Medical Center. For details please contact the supervisor Elaine Tanji at etanji@u.washington.edu (206) 598-6030. -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From gcallis <@t> montana.edu Thu Sep 28 10:16:25 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 28 10:16:29 2006 Subject: [Histonet] VCAM-1, ICAM-1, P-selectin antibody In-Reply-To: <000f01c6e2d4$942a92c0$0ec8a8c0@your56ab121f6e> References: <000f01c6e2d4$942a92c0$0ec8a8c0@your56ab121f6e> Message-ID: <6.0.0.22.1.20060928091232.01b5b958@gemini.msu.montana.edu> We buy our antibodies from BD Pharmingen, and do only frozen sections on fresh snap frozen tissues. You can do either enzyme immunohistochemistry or delightful double immunfluorescence staining if you want to see colocalization of the antigens. These antibodies may NOT work on formalin fixed paraffin seftions. Go to BD Pharmingens website for pricing, quantities, and also product data sheets for particulars on these antibodies. At 02:03 AM 9/28/2006, you wrote: >Hello... > >I need some recomendation to choose antibody for VCAM-1, ICAM-1 and >P-selectin detection on mouse vascular tissue either processed as frozen >or paraffin embedded section. I found so many available products and I >have difficulties in choosing. If anyody have experience, please give me info. > >Thanks, > >Wulan >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Jerry <@t> ralambusa.com Thu Sep 28 10:18:14 2006 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Thu Sep 28 10:18:34 2006 Subject: [Histonet] cassette writers Message-ID: <3855F92002259948A66A8CA2D16E3A4F0177A2@server.ralambusa.com> Alan, I would definitely recommend anyone of the Thermo, TBS or RA Lamb units, depending on your needs, they all have different advantages. Alan, We tested three different units this year, Sakura, TBS and Thermo. The thermo unit was the only one that impressed us as to reliability. The other two jammed up quite a bit. Needless to say, my thermo unit is now on order. Charles Embrey, PA(ASCP),HT Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Bishop Sent: Wednesday, September 27, 2006 9:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette writers Anyone got any recomendation for cassette writers? Need to go down that route by the end of the year but don't really know what is on offer at the moment and what is good or bad! All input appreciated. Cheers Alan Bishop Charge Scientist Histopathology Medlab Central Palmerston North New Zealand From celebrej <@t> HHSC.CA Thu Sep 28 10:40:27 2006 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Thu Sep 28 10:40:38 2006 Subject: [Histonet] Staining for TB Message-ID: <0B3BF9D810C7094FA34B17DD145C3D5E02B0D023@ipemail01.hhsc.ca> Hello Netters... Just curious to know how everybody does their ZN special stain? We have been told it is not recomended to perform this stain in a coplin jar, that the slides should be stained individually to avoid possible cross contamination from the control slide. Is this a proven fact ? Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From gcallis <@t> montana.edu Thu Sep 28 10:45:07 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 28 10:45:14 2006 Subject: [Histonet] Re: effects of elevation on immunohistochemistry In-Reply-To: References: Message-ID: <6.0.0.22.1.20060928093611.01b520d8@gemini.msu.montana.edu> Lisa, At 4800 ft above sea level, nothing on actual staining for application of reagents. However, it may affect your HIER or heated retrievals since water boils at a lower temperature at high altitude. I would assume one might have to establish retrieval time if deemed a problem. Altitude was discussed on Hitonet many years ago (check archives). Patsy Ruegg, who works at an even higher elevation in Colorado addressed this point. You should also ask the Ventana tech services too - some of them came from Flagstaff AZ, which is REALLY high altitude and their company is in Arizona. Hopefully Liz Chlipala will address this issue since she is in Colorado. I never do retrievals, but my staining protocols without problems. At 08:21 AM 9/28/2006, you wrote: >Good morning, > Does anyone have any experience or knowledge about the effects of > elevation on immunohistochemistry? > Thanks kindly, Lisa Manning Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From lloyd.3 <@t> osu.edu Thu Sep 28 10:51:07 2006 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Thu Sep 28 10:51:12 2006 Subject: [Histonet] Expiration dates on acids Message-ID: <2096B6FC591D034FA50AFFD7A42EE9650184C676@dental.dentnet.dent.ohio-state.edu> From lloyd.3 <@t> osu.edu Thu Sep 28 10:52:39 2006 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Thu Sep 28 10:52:43 2006 Subject: [Histonet] Expiration dates on acids Message-ID: <2096B6FC591D034FA50AFFD7A42EE9650184C677@dental.dentnet.dent.ohio-state.edu> I am getting ready for a Clia inspection. Where do I find expiration dates for acids so I can decide if my acids are out of date or not. Thanks Mary From pruegg <@t> ihctech.net Thu Sep 28 11:13:17 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Sep 28 11:13:33 2006 Subject: [Histonet] Re: effects of elevation on immunohistochemistry In-Reply-To: <20060928144459.33480.qmail@web61218.mail.yahoo.com> Message-ID: <004901c6e319$0523d600$6601a8c0@Patsy> HIER is affected at altitude and one of the big problems we ran into in CO was FDA compliance with the Her2 kit from DAKO which required HIER be carried out using a waterbath and the temp requirement was 95c for 45', well her in Denver and especially in Colorado Springs which is even higher our water boils rapidly at 92-93C, if you leave your container in a rapidly boiling WB for 45' all the water boils off and you still don't reach the required temp for the required time. We tried many solutions to compli with FDA, people built elaborate lids to seal the WB so that at least the water would not boil off, I tested pressure cookers extensively, and although it was thought that with the pressure being controlled the water should not boil violently my experience seemed to indicate that the water was boiling violently because the sections were really damaged in the pressure cooker compared to WB. I am not sure what DAKO finally agreed upon for Her2, I think they extended the time in the WB for those of us at altitude, I know it took years for anyone to even address the issue and frankly I have moved on and not kept up, I think it just points to the problem of physically not being able to always comply with FDA in certain areas. You may have noticed that the FDA approved EGFR kit from DAKO uses enzyme digestion instead of HIER. It never ceases to amaze me how tests that only tested and developed at sea level can get FDA certification without any consideration of others at higher altitudes. I asked the FDA this question at a CAP conference on Her2 in Chicago and they completely blew me off as if how could it matter. The fda made the statement that the her2 kit was tested from the east coast of the US to the west coast and this is when I questioned them about the higher altitude in the middle which obviously was not tested and they seemed to just say we tested in New York and California and assumed it work the same everywhere in between. On a more recent note. I did test the Lab Vision enclosed waterbath HIER instrument and was able to get it to 95dc and hold there for 45' without any obvious violence done to the tissue sections. Patsy Ruegg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 28, 2006 8:45 AM To: Lisa Manning; histonet@lists.utsouthwestern.edu; Sundi Readlinger Subject: Re: [Histonet] Re: effects of elevation on immunohistochemistry Lisa: This is a Physics issue! At higher elevation atmosferic pressure is lower and the water boils at less than 100?C so any HIER done in an "open" container, like heating in the MW oven or using a steamer or a water bath, will be affected because 100?C cannot be reached. HIER should be done in a pressurized environment (like a pressure cooker). There was a paper published in the Jorunal of Histotechnology not too long ago (I do not have the exact reference) dealing with Her-2 Neu tests at high elevation. Any step requiring a certain temperature in an open container, will be affected. Other steps temperature independednt will not be affected. Hope this will help you! Ren? J. Lisa Manning wrote: Good morning, Does anyone have any experience or knowledge about the effects of elevation on immunohistochemistry? Thanks kindly, Lisa Manning Lisa Manning R.T, B.Sc. Histopathology Biologist Canadian Food Inspection Agency National Centre for Foreign Animal Disease 1015 Arlington Avenue, Suite T2300 Winnipeg, Manitoba R3E 3M4 PH. (204) 789-2087 FAX (204)789-2038 email: manningl@inspection.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Sep 28 11:30:28 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Sep 28 11:26:06 2006 Subject: [Histonet] elevation and HIER In-Reply-To: <6.0.0.22.1.20060928091232.01b5b958@gemini.msu.montana.edu> Message-ID: <000001c6e31b$698a47d0$0300a8c0@domain.Premier> I'll chime in here also. We currently use the pascal pressure cooker for most of our HIER protocols, prior to purchasing the pascal we used a black and decker steamer, we still use the black and decker steamer on occasion, mostly for specimens which are a bit friable and have a tendency to fall off the slide during retrieval. But there are issues with the steamer. In the steamer and at my elevation I can only get the retrieval buffer to get to a temperature of 93 to 93.5 degrees Celsius, that temp can work for some immunos, but using the steamer can cause problems with consistency if you do not monitor the temperature of your retrieval buffer through the entire process. I learned this the hard way. It my opinion in order to use a steamer at elevation and to maintain any consistency with respects to your results you need to monitor temp through the entire process. You can not just start your steamer wait about 20 to 30 minutes and assume that its up to the correct temp, put your slides and then time for 20 mintues, that's not going to work consistently. When we use the steamer we use a digital probe thermometer that fits through the holes in the top of the steamer and directly into the retrieval buffer. Once the temp hits 93 + degrees we add the slides and continue to monitor the temp, when the temp gets back up to 93 + degrees we start our timer. That protocol seems to work well for the steamer. We much prefer using the pascal since its easy and does not take that long as compared to the steamer which can take up to 1.5 to 2 hours to complete the entire retrieval process. I have not used a microwave or water bath at elevation so I can not comment on those methods. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, September 28, 2006 9:16 AM To: Wulan Anggrahini; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] VCAM-1, ICAM-1, P-selectin antibody We buy our antibodies from BD Pharmingen, and do only frozen sections on fresh snap frozen tissues. You can do either enzyme immunohistochemistry or delightful double immunfluorescence staining if you want to see colocalization of the antigens. These antibodies may NOT work on formalin fixed paraffin seftions. Go to BD Pharmingens website for pricing, quantities, and also product data sheets for particulars on these antibodies. At 02:03 AM 9/28/2006, you wrote: >Hello... > >I need some recomendation to choose antibody for VCAM-1, ICAM-1 and >P-selectin detection on mouse vascular tissue either processed as frozen >or paraffin embedded section. I found so many available products and I >have difficulties in choosing. If anyody have experience, please give me info. > >Thanks, > >Wulan >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1781 (20060928) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From MSHERWOOD <@t> PARTNERS.ORG Thu Sep 28 11:40:56 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Sep 28 11:41:10 2006 Subject: [Histonet] VCAM-1, ICAM-1, P-selectin antibody In-Reply-To: <000f01c6e2d4$942a92c0$0ec8a8c0@your56ab121f6e> Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30C50@PHSXMB1.partners.org> I use these antibodies, as well as E-selectin, which are specific for various cell-adhesion molecules (CAMs) for a study on immuno-suppressed patients. We use them on frozen sections of punch biopsies of skin. I ordered my antibodies from DAKO. (I should add that I have not had any problems with ordering from DAKO). Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wulan Anggrahini Sent: Thursday, September 28, 2006 4:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VCAM-1, ICAM-1, P-selectin antibody Hello... I need some recomendation to choose antibody for VCAM-1, ICAM-1 and P-selectin detection on mouse vascular tissue either processed as frozen or paraffin embedded section. I found so many available products and I have difficulties in choosing. If anyody have experience, please give me info. Thanks, Wulan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Sep 28 15:01:30 2006 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Thu Sep 28 15:01:54 2006 Subject: [Histonet] Staining for TB Message-ID: yes. since the organism creates an environment of caseous necrosis around itself and in which it lives, it is entirely possible that organisms could contaminate a coplin jar of stain and although non-viable, be left floating where they can adhere to subsequent slides. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Celebre Julia" 09/28/06 11:40AM >>> Hello Netters... Just curious to know how everybody does their ZN special stain? We have been told it is not recomended to perform this stain in a coplin jar, that the slides should be stained individually to avoid possible cross contamination from the control slide. Is this a proven fact ? Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shu-cheng.chen <@t> spcorp.com Thu Sep 28 15:05:17 2006 From: shu-cheng.chen <@t> spcorp.com (Chen, Shu-Cheng) Date: Thu Sep 28 15:05:24 2006 Subject: [Histonet] histopathology service Message-ID: <886D951F4246D94DAF28C82DC102DDD0024F180E@KENMSG20.us.schp.com> Hi, I am looking for CROs that would do contract pathology work for animal disease models. Can you please either contact me directly or give me suggestions. Thank you for your help. Shu-Cheng ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From srousselle <@t> hsrl.org Thu Sep 28 15:53:38 2006 From: srousselle <@t> hsrl.org (Serge Rousselle) Date: Thu Sep 28 15:54:59 2006 Subject: [Histonet] histopathology service Message-ID: <200609282054.k8SKsgqA012279@sablefish.shentel.net> Dear Chen, HSRL is a full service contract histopathology organization. We can accommodate any of your histology and pathology needs, including animal model evaluations. I was recently working at Charles River Labs and did a lot of different types of evaluations, including animal models, but also implants, toxicology, efficacy studies, etc. Please visit our web site (www.hsrl.org ). The pathology page is still pending but that will give you an idea of who we are and what our client services are like. We are very responsive, strive for excellence, and have very competitive prices. I will be happy to send you my CV upon request. I hope we can discuss your projects, Best regards, Serge Serge D. Rousselle, DVM, DACVP Director of Pathology - Senior Pathologist HSRL (Histo-Scientific Research Laboratories) 5930 Main Street Mount Jackson, VA 22842 540 335 5638 (Mobile) 540 477 4440 540 477 4448 (Fax) srousselle@hsrl.org From gcallis <@t> montana.edu Thu Sep 28 16:13:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 28 16:13:14 2006 Subject: [Histonet] Precise fixed tissue slices using a CutMATE forceps Message-ID: <6.0.0.22.1.20060928144519.01b69d18@gemini.msu.montana.edu> A recent inquiry asked for devices to hold tissues for precise, although 1 mm thick slices of tissue. Mopec CutMATE forceps are designed to hold tissues for precise slicing. The forceps resembles a paddle with slots in the tissue griping area. One inserts a sharp blade into slots, above firmly held tissue, and then cut serial slices. There are forceps for 2, 3, and 4 mm thick slices, or you can purchase a set. The handles are ergonomically designed. Go to the company website at www.mopec.com Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Eric <@t> ategra.com Thu Sep 28 23:34:02 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Thu Sep 28 23:19:28 2006 Subject: [Histonet] Temporary and Permanent Histology opportunites near you Message-ID: Hi - Fellow-Histonetters How are things there in MN/TX/CA/MA/USA ? Below is the updated list of both temp and perm Histology jobs, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech jobs are permanent full-time unless indicated otherwise Also, if your not interested in any of these great opportunities and perfer a different location, please call me and let me know where and I can find it for you. Permanent Histology Jobs: ------------------------------------------------------ 1. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 2. Washington, D.C - Histotech -perm and temp 3. Northern New Jersey - HistoTech - perm 4. Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech 5. Boston Mass - HistoTech - HistoTech -perm 6. Massachusetts (North of Boston) - perm - Bench Histotech 7. Central Florida -perm- Histotech 8. Southeast Florida - Treasure Coast - perm - HistoTech 9. Southeast Florida - perm - HistoTech 10. Southwest Florida - perm - Bench Histotech/ Supervisor 11. Florida, West Coast - both temp & perm openings- Bench Histotech 12. New Hampshire perm openings - Bench Histotech (opportunity to learn Mohs!) 13. New York ( Syracuse area) - Bench Histotech- perm 14. Central Florida - Bench Histotech- perm 15. New York (Long Island)- Bench Histotech- perm -----------------------------------end perm jobs ---------------------------------------------------- Temporary Assignments ------------------------------- 1. Alaska -Histo Tech Temp - minimum of 16 weeks 2. Washington,DC - HistoTech - minimum of 6 weeks- also Temp to Perm 3. Massachusetts (Boston area) - Histo Tech minimum of 13 weeks (2 people) 4. Pennsylvania - HistoTech- 6 months 5. New Mexico - Histotech- 10-12 weeks 6. Florida (South East)(Treasure Coast)- minimum of 4 weeks possibly longer -------------------------- end list of HistoTech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From jqb7 <@t> cdc.gov Fri Sep 29 05:49:32 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Fri Sep 29 05:54:39 2006 Subject: [Histonet] Automated special stains Message-ID: Hi all, Any feedback on the NexES special stains machine? Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From ryeo <@t> wchosp.org Fri Sep 29 07:31:16 2006 From: ryeo <@t> wchosp.org (Richard Yeo) Date: Fri Sep 29 07:29:35 2006 Subject: [Histonet] Commercial kits Message-ID: Does anyone out there in histo-land know if there is a commercial kit available for detecting anionic detergent residue on glassware that has been cleaned? Any help will be greatly appreciated. My pathlological does not, for some reason like the pH meter test or the litmus paper test. He said there should be a kit available to check the glass-ware. From doug <@t> ppspath.com Fri Sep 29 07:38:24 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Sep 29 07:36:11 2006 Subject: [Histonet] Automated special stains In-Reply-To: Message-ID: I have no complaints about it. You can run multiple stains at the same time. It is easy to use and you can walk away from it. The only service that I needed was PM's. I recommend it. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCID) Sent: Friday, September 29, 2006 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated special stains Sensitivity: Confidential Hi all, Any feedback on the NexES special stains machine? Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikael.niku <@t> helsinki.fi Fri Sep 29 08:23:52 2006 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Sep 29 08:24:02 2006 Subject: [Histonet] Nonspecific staining in enterocyte cytoplasm Message-ID: <451D1E68.5060400@helsinki.fi> Dear Histonetters, I'm looking for an explanation for a peculiar type of nonspecific staining in the small intestine: with several monoclonal antibodies, we get intensely stained granules or vacuoles in the enterocyte cytoplasm, quite specifically localizing at a small area on the apical side of the nucleus. The nucleus itself, the most apical cytoplasm and the plasma membrane are clear. The staining appears to depend on the primary antibody, but occurs with several presumably independent monoclonals which otherwise work fairly nicely. It is somewhat exaggerated by alkaline antigen retrieval but unaffected by biotin or peroxidase blocking. We are routinely using a serum block, and include BSA in the antibody dilutions, but haven't tried other blocking methods against nonspecific antibody binding so far. This is about paraformaldehyde-fixed paraffin sections of bovine tissues. With best regards, Mikael -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From gu.lang <@t> gmx.at Fri Sep 29 09:54:00 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 29 09:54:06 2006 Subject: AW: [Histonet] Automated special stains In-Reply-To: Message-ID: <000001c6e3d7$196ff800$eeeea8c0@dielangs.at> It is good for small numbers of special stains and if you have no special modifications. Twenty positions for slides, twenty-five positions for reagens. A trichrom needs 10 places and takes ca. 90 min. So with a few different stains you have to make more runs, what is time-consuming. Now we need sometime the whole morning to make 15 stains, formerly we did it in one and a half hour by hand... But you need very little place for reagens-storage, you can go away, it is reliable, the silverstains are very good, it is easy to handle (no brain needed), the results are each time the same. It is not cheap and sometime the reagens-time is over before all tests are consumed. Our younger technicians haven't done a simple Alcianblue by hand since we have this automat (my histo-heart is crying). Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bartlett, Jeanine (CDC/CCID/NCID) Gesendet: Freitag, 29. September 2006 12:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Automated special stains Vertraulichkeit: Vertraulich Hi all, Any feedback on the NexES special stains machine? Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SohrabB1 <@t> wmmcpo.ah.org Fri Sep 29 11:49:26 2006 From: SohrabB1 <@t> wmmcpo.ah.org (Behnaz Sohrab) Date: Fri Sep 29 11:50:11 2006 Subject: [Histonet] Competency Message-ID: I was wondering what kind of forms or test one can do for histologists competency testing for Cap.? Thanks, Behnaz, histo supervisor From dchihc <@t> hotmail.com Fri Sep 29 12:39:52 2006 From: dchihc <@t> hotmail.com (Phyllis Thaxton) Date: Fri Sep 29 12:39:56 2006 Subject: [Histonet] Job Opening In Alabama Message-ID: Primary Duties/Responsibilities: Under proper supervision, performs a variety of tasks and functions of a technical nature making independent decisions as needed. Must have a working knowledge of histology, pathology equipment/instruments and computers. IHC and frozen section experience desired. Minimum Qualifications: Must be certified by the American Society of Clinical Pathologists (ASCP) as an HT or HTL. Must possess excellent human relation skills and the ability to perform at a high degree of efficiency, accuracy and effectiveness. Must be able to perform the duties of a Histology Technician to include age specific laboratory needs of the neonatal, pediatric, adolescent, adult and geriatric patients. Classification: Full Time Shift: Day shift Contact: Sherrie Faulkner Email: sfaulkner@dchsystem.com _________________________________________________________________ The next generation of Search—say hello! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLMTAG From mtarango <@t> nvcancer.org Fri Sep 29 13:34:51 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Sep 29 13:35:12 2006 Subject: [Histonet] Competency Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1C6EB@NVCIEXCH02.NVCI.org> Try using a CAP survey to demonstrate competency. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, September 29, 2006 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Competency I was wondering what kind of forms or test one can do for histologists competency testing for Cap.? Thanks, Behnaz, histo supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From rjbuesa <@t> yahoo.com Fri Sep 29 14:04:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 29 14:04:58 2006 Subject: [Histonet] Competency In-Reply-To: Message-ID: <20060929190454.57581.qmail@web61223.mail.yahoo.com> Behnaz Sohrab: I developed standards of performance and competency requirements for each task within the histology workflow and once a year I checked them against each HT (usually near their evaluation date). Those checked forms were kept on each personal file and presented to the CAP inspectors if requested. They always found this as a very good procedure. Hope this will help you! Ren? J. Behnaz Sohrab wrote: I was wondering what kind of forms or test one can do for histologists competency testing for Cap.? Thanks, Behnaz, histo supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From sohail_e <@t> yahoo.com Fri Sep 29 15:06:27 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Fri Sep 29 15:06:31 2006 Subject: [Histonet] Problem of Double immunoflouresence staining of retinal vasculature Message-ID: <20060929200627.949.qmail@web39506.mail.mud.yahoo.com> Hello everybody I have a big problem of staining retina vasculature using a cocktail of two different antibodies. The problem is that i can only see staining with FITC but not with TRITC, hence i cant make a merger of both FITC and TRITC. Cocktail of primary antibodies include (1) Monoclonal Rabbit Anti human vWF (2) Monoclonal mouse Anti alpha SMA and cocktail of secondary antibodies include (1) Anti rabbit FITC (2) Anti mouse TRITC Please let me know your commentc to slove this issue. Dr.Sohail --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From mtarango <@t> nvcancer.org Fri Sep 29 15:49:33 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Sep 29 15:49:59 2006 Subject: [Histonet] Commercial kits Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1C6F1@NVCIEXCH02.NVCI.org> Phenolphthalein Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Yeo Sent: Friday, September 29, 2006 5:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Commercial kits Does anyone out there in histo-land know if there is a commercial kit available for detecting anionic detergent residue on glassware that has been cleaned? Any help will be greatly appreciated. My pathlological does not, for some reason like the pH meter test or the litmus paper test. He said there should be a kit available to check the glass-ware. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From beldorth.msu+hist <@t> gmail.com Fri Sep 29 18:22:12 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Fri Sep 29 18:22:16 2006 Subject: [Histonet] Need major help with In Situ . . . Message-ID: Hi All, I am working on a project involving embryonic Sea Lamprey and am having many difficulties with In Situ. Namely, the tissue sections (frozen cryo-sections) are washing off the slides (mostly during the 68oC, 2x and 0.1x SSC washes, but anything that is left washes off before they are coverslipped), all of them, 100% loss. Everything worked fine at first, but then I started losing tissue and have not been able to isolate the problem and develop a cure. The embryos were killed, fixed overnight in 4% Paraformaldehyde, then placed in absolute methanol at -80oC. Before use I bring them to water (100%, 95%, 70% ethanol, diH20, 2x 5mins each) then soak overnight in 20% Sucrose, then soak 4hrs-overnight in OCT compound before freezing. After sectioning I dry overnight at 40oC before performing ISH. I started using Fisher SuperFrost Plus and have tried APES treated slides (2% APES in acetone for 15min, wash in acetone, wash in diH2O, dried overnight at 50oC) with no luck. I have tired working with them laying flat (the slides, not me) rather than using vertically-situated staining jars, and using a barrier pen instead of gasketed coverwells to hold solutions (the suction created while removing the coverwells pulls sections off the slide). The latest attempt involved staining/counterstaining the entire embryo first, then cryo-sectioning and air drying the slides overnight, then clarifying and mounting. This worked great, lots of tissue on the slides, buuuutttt . . .the counterstain (Nuclear Fast Red) washed out while soaking overnight in 20% Sucrose prior to freezing. In addition the stain (NBT/BCIP) looks, well, not good. Smeared, or spilled (as in, 'spilled out' of the cells) may be a way to describe it. So in summary: Procedure: In Situ Hybridization DIG/Alkaline Phosphatase labeled RNA probes NBT/BCIP stain Nuclear Fast Red Counterstain Vectamount Permanent mounting media Problem: tissue falling off slides I am at a complete loss. Has anyone out there in Histoland worked with similar tissues or had a similar problem, and how did you fix it? I have heard of using RNase A after Hybridization in place of high-temp, high stringency SSC washes, but have not tired it (we do a lot of work with RNA in my lab, and I am a bit worried about working with RNase). Can anyone recommend this procedure? Hell, can anyone recommend ANYTHING? I do not know how to proceed from here, and your help is dearly appreciated. IB From beldorth.msu+hist <@t> gmail.com Fri Sep 29 18:22:50 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Fri Sep 29 18:22:52 2006 Subject: [Histonet] Water stable counterstain? Message-ID: Hi All, I just sent a long message about ISH, but I have another question perhaps someone can answer. I am using Nuclear Fast Red (Vector Labs) to counter stain tissue stained with NBT/BCIP (also Vector Labs), and, in order to reduce the number of steps in the protocol, I would like to use an aqueous based mounting media (at least with the aqueous media I have tried). However, Nuclear Fast Red washes out of the tissue when mounted aqueously, making contrast under the microscope terrible. Can anyone 1) recommend a counterstain, preferably something red, that will be stable in aqueous mounting media, i.e. not wash out? 2) recommend a water-stable non-red counterstain that has excellent contrast to NBT/BCIP ( i.e., is there a different color counterstain that is compatible with NBT AND aqueous mounting media?) 3) recommend a completely different substrate/counterstain combination that works with DIG/Alkaline Phosphatase and that can be mounted aqueously? ( i.e., if the NBT/BCIP just plain sucks, and I am getting the impression that it does, is there anything out there I can use with Alkaline Phosphatase so I don't have to completely rework my probes?) Thanks, IB From asmith <@t> mail.barry.edu Sat Sep 30 10:06:52 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sat Sep 30 10:06:58 2006 Subject: [Histonet] Water stable counterstain? In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E3FD3@exchsrv01.barrynet.barry.edu> There are two silver stains for nuclei that are completely water-resistant: Korson's 1964 (J. Histochem. Cytochem. 12: 875) methanamine-silver method for DNA and Black and Ansley's 1964 (Science 143: 693) ammoniacal silver method for histones. It has been 35 years since I used either method, but my recollection is that Korson's methanamine-silver is extremely fussy and time-consuming. Black and Ansley's ammoniacal silver is quite tricky at first, but fairly simple once you get the hang of it. Mayer's paracarmine (p. 84 of the 2nd edition or p. 215 of the 4th edition of Lillie's HISTOPATHOLOGIC TECHNIC) calls for 70% alcohol for the staining solution, but the resulting stain is water-resistant. I used to make up a stock solution in 70% alcohol and dilute it to 50% alcohol for staining. Other alum carmine methods would probably also give water-resistant staining. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I.B. Sent: Friday, September 29, 2006 7:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water stable counterstain? Hi All, I just sent a long message about ISH, but I have another question perhaps someone can answer. I am using Nuclear Fast Red (Vector Labs) to counter stain tissue stained with NBT/BCIP (also Vector Labs), and, in order to reduce the number of steps in the protocol, I would like to use an aqueous based mounting media (at least with the aqueous media I have tried). However, Nuclear Fast Red washes out of the tissue when mounted aqueously, making contrast under the microscope terrible. Can anyone 1) recommend a counterstain, preferably something red, that will be stable in aqueous mounting media, i.e. not wash out? 2) recommend a water-stable non-red counterstain that has excellent contrast to NBT/BCIP ( i.e., is there a different color counterstain that is compatible with NBT AND aqueous mounting media?) 3) recommend a completely different substrate/counterstain combination that works with DIG/Alkaline Phosphatase and that can be mounted aqueously? ( i.e., if the NBT/BCIP just plain sucks, and I am getting the impression that it does, is there anything out there I can use with Alkaline Phosphatase so I don't have to completely rework my probes?) Thanks, IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From gu.lang <@t> gmx.at Sat Sep 30 11:49:23 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Sep 30 11:49:24 2006 Subject: AW: [Histonet] Need major help with In Situ . . . In-Reply-To: Message-ID: <000301c6e4b0$621c32b0$eeeea8c0@dielangs.at> I have no answer for your problem, but I'm curious, why the embryos must be fixed before freezing and cutting. I have in my mind that fixed, frozen tissue sticks not really well on the slides. I remember darkly, that cryosections of fixed tissue were stained free floating in small dishes, and at last were mounted on a slide, what wasn't really easy. If your procedure first worked with the Superfrost slides, perhaps their shelflife is over? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von I.B. Gesendet: Samstag, 30. September 2006 01:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Need major help with In Situ . . . Hi All, I am working on a project involving embryonic Sea Lamprey and am having many difficulties with In Situ. Namely, the tissue sections (frozen cryo-sections) are washing off the slides (mostly during the 68oC, 2x and 0.1x SSC washes, but anything that is left washes off before they are coverslipped), all of them, 100% loss. Everything worked fine at first, but then I started losing tissue and have not been able to isolate the problem and develop a cure. The embryos were killed, fixed overnight in 4% Paraformaldehyde, then placed in absolute methanol at -80oC. Before use I bring them to water (100%, 95%, 70% ethanol, diH20, 2x 5mins each) then soak overnight in 20% Sucrose, then soak 4hrs-overnight in OCT compound before freezing. After sectioning I dry overnight at 40oC before performing ISH. I started using Fisher SuperFrost Plus and have tried APES treated slides (2% APES in acetone for 15min, wash in acetone, wash in diH2O, dried overnight at 50oC) with no luck. I have tired working with them laying flat (the slides, not me) rather than using vertically-situated staining jars, and using a barrier pen instead of gasketed coverwells to hold solutions (the suction created while removing the coverwells pulls sections off the slide). The latest attempt involved staining/counterstaining the entire embryo first, then cryo-sectioning and air drying the slides overnight, then clarifying and mounting. This worked great, lots of tissue on the slides, buuuutttt . . .the counterstain (Nuclear Fast Red) washed out while soaking overnight in 20% Sucrose prior to freezing. In addition the stain (NBT/BCIP) looks, well, not good. Smeared, or spilled (as in, 'spilled out' of the cells) may be a way to describe it. So in summary: Procedure: In Situ Hybridization DIG/Alkaline Phosphatase labeled RNA probes NBT/BCIP stain Nuclear Fast Red Counterstain Vectamount Permanent mounting media Problem: tissue falling off slides I am at a complete loss. Has anyone out there in Histoland worked with similar tissues or had a similar problem, and how did you fix it? I have heard of using RNase A after Hybridization in place of high-temp, high stringency SSC washes, but have not tired it (we do a lot of work with RNA in my lab, and I am a bit worried about working with RNase). Can anyone recommend this procedure? Hell, can anyone recommend ANYTHING? I do not know how to proceed from here, and your help is dearly appreciated. IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Sat Sep 30 14:01:02 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Sat Sep 30 14:01:07 2006 Subject: [Histonet] Job Opening Message-ID: <6BB8BC4519AAB844B174FC739A679BBC50900C@IRMEXCH01.irm.inhs.org> Hello All, Deaconess is a 300 bed acute care community medical center. We have a full time, M-F, day time position open. Must be HT(ASCP) certified or eligible. Duties include all routine histology functions in a clinical setting (i.e. embedding , cutting, special stains and frozen sections). IHC experience preferred. Due to a recent market adjustment by our organization the salary range for this position has just increased 25-33% depending on years of experience. This is a golden opportunity for someone to improve on their current situation or to begin anew. Anyone new to this field is encouraged to apply as well, for the door is wide open. You can apply on-line by going to our corporate website www.empirehealth.org. The position is at Deaconess Medical Center in Spokane, WA www.deaconess-spokane.org. Please contact me directly for more details. Following is an additional web link to provide you with more info on Spokane and the greater Inland Northwest region www.visitspokane.com. ("Near Perfect-Near Nature"). Thank you and best wishes, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconess-spokane.org From jschwamborn <@t> yahoo.de Tue Sep 19 10:57:11 2006 From: jschwamborn <@t> yahoo.de (Jens Schwamborn) Date: Mon Oct 9 12:54:20 2006 Subject: [Histonet] Fixation of embryonic mouse brain for cryosections Message-ID: <20060919155708.48902.qmail@web26806.mail.ukl.yahoo.com> Dear Histonet-people, I want to image EGFP fluorescence (endogenous) in cryosections of embryonic mouse brains (stages: E12.5 to E18.5). Furthermore I would like to perform antibody-stainings for immunofluorescence on those sections. Now I am searching for the best protocol to do so. Do you fix the brains before the sections? If yes, how? (I guess perfusion is impossible at E12.5) Or do you freeze the complete brain, perform the sectioning and fix afterwards? If so, how do you freeze, with dry ice ? Thanks for the help. Jens email: jschwamborn@yahoo.de --------------------------------- Jetzt Mails schnell in einem Vorschaufenster ?berfliegen. Dies und viel mehr bietet das neue Yahoo! Mail . From vanann702 <@t> skmc.gov.ae Tue Sep 19 12:04:27 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Mon Oct 9 12:54:24 2006 Subject: [Histonet] help Message-ID: I have been asked by a colleague to source a paper/article on tissue artefacts from the 70's, author E A Wallington does anyone have info for me? please TIA Annie