From Melissa.Gonzalez <@t> cellgenesys.com Sun Oct 1 10:40:44 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Sun Oct 1 10:40:52 2006 Subject: [Histonet] RE: Problem of Double immunoflouresence staining .. Message-ID: Sohail, First, Perhaps you should try incubating each primary with respective secondary only, to determine how they look separately, and also make sure each is working on its own. For example, you may not have your aSMA dilution correct. Also, do you have a positive control for TRITC to make sure you can detect any TRITC signal adequately? Once you have appropriate signal from both, you can then make your cocktails. Also, in the future, I would recommend switching to Alexa Fluors from Molecular Probes, they really are great. In your instance you would use AF488 (FITC) and AF 546 or 555 (TRITC). But that is just my 2 cents. Good luck, Melissa Message: 4 Date: Fri, 29 Sep 2006 13:06:27 -0700 (PDT) From: sohail ejaz Subject: [Histonet] Problem of Double immunoflouresence staining of retinal vasculature To: histonet@lists.utsouthwestern.edu Message-ID: <20060929200627.949.qmail@web39506.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello everybody I have a big problem of staining retina vasculature using a cocktail of two different antibodies. The problem is that i can only see staining with FITC but not with TRITC, hence i cant make a merger of both FITC and TRITC. Cocktail of primary antibodies include (1) Monoclonal Rabbit Anti human vWF (2) Monoclonal mouse Anti alpha SMA and cocktail of secondary antibodies include (1) Anti rabbit FITC (2) Anti mouse TRITC Please let me know your commentc to slove this issue. Dr.Sohail From phiho.hoang <@t> rogers.com Sun Oct 1 10:47:56 2006 From: phiho.hoang <@t> rogers.com (PhiHo Hoang) Date: Sun Oct 1 10:47:56 2006 Subject: [Histonet] The procedure that substitues xylene with mineral oil. Message-ID: <005b01c6e570$f725b820$f300a8c0@ttth> Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Ren? J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Ren? J. Sharon Allen exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo From rjbuesa <@t> yahoo.com Sun Oct 1 11:55:53 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Oct 1 11:55:57 2006 Subject: [Histonet] The procedure that substitues xylene with mineral oil. In-Reply-To: <005b01c6e570$f725b820$f300a8c0@ttth> Message-ID: <20061001165553.32345.qmail@web61219.mail.yahoo.com> PhiHo: I am attaching the procedure under separate cover. Ren? J. PhiHo Hoang wrote: Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Ren? J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Ren? J. Sharon Allen exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From phiho.hoang <@t> rogers.com Sun Oct 1 12:23:31 2006 From: phiho.hoang <@t> rogers.com (PhiHo Hoang) Date: Sun Oct 1 12:23:30 2006 Subject: [Histonet] The procedure that substitues xylene with mineral oil. In-Reply-To: <20061001165553.32345.qmail@web61219.mail.yahoo.com> Message-ID: <006a01c6e57e$513dc340$f300a8c0@ttth> Hi Ren?, You wrote: > I am attaching the procedure under separate cover. > Ren? J. Your help is very much appreciated. I am reading your paper. Best regards, PhiHo _____ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Sunday, October 01, 2006 12:56 PM To: PhiHo Hoang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The procedure that substitues xylene with mineral oil. PhiHo: I am attaching the procedure under separate cover. Ren? J. PhiHo Hoang wrote: Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Ren? J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Ren? J. Sharon Allen <@t>exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From Shirley_PHUA <@t> hsa.gov.sg Sun Oct 1 13:06:36 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Oct 1 13:06:48 2006 Subject: [Histonet] Shirley is away, attending a workshop in Bangkok : 02 & 03 October 2006 (Monday & Tuesday) Message-ID: I will be out of the office from 02-10-2006 to 03-10-2006. I will return on 04 October 2006 (Wednesday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From arvidsonkristen <@t> yahoo.com Sun Oct 1 15:19:17 2006 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sun Oct 1 15:19:22 2006 Subject: [Histonet] Fwd: FW: fried skin specimens Message-ID: <20061001201917.34929.qmail@web61316.mail.yahoo.com> Note: forwarded message attached. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From anh2006 <@t> med.cornell.edu Mon Oct 2 01:37:51 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Oct 2 01:37:58 2006 Subject: [Histonet] Anti-mouse CD146 Message-ID: Does anyone know where to find this antibody and if it will stain mouse tissue in a similar way to how P1H12 stains human tissue (EC marker?). Thanks. -- From f.finlay <@t> formed.gla.ac.uk Mon Oct 2 04:40:29 2006 From: f.finlay <@t> formed.gla.ac.uk (Finlay Finlay) Date: Mon Oct 2 04:40:34 2006 Subject: [Histonet] cassette pens Message-ID: Hi Histonetters, This posting is for everyone who uses pens to mark histology cassettes. When we tried to replace our favourite Statmark pens, we discovered that we had to order them through Fisher in USA ( we are in Glasgow, Scotland) and would have to wait for weeks for the order to come through. In the meantime we ordered Secureline from VWR and they faded very badly! Good old fashioned pencil would have been better. Searching Histonet archives I found many complaints about these pens, and decided to complain to the manufacturer , Precision Dynamics. Their international sales coordinator , Ariane Bras has passed my comments on , but I wondered if anyone else who has had problems with these markers would send their views to her [abras@pdc-europe.com] maybe if enough people complain, they will make pens that are fit for purpose. We have now got our Statmark delivery from Fisher, it came through quite quickly, But does anyone know where to buy these in the UK? From dmccaig <@t> ckha.on.ca Mon Oct 2 08:20:38 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Oct 2 08:26:40 2006 Subject: [Histonet] TRANSFERRING SECTIONS FROM REGULAR TO CHARGED SLIDES Message-ID: Can someone share with me the procedure for transferring sections dried on a regular slide to a frosted slide to prevent possible lifting when special staining. Thanks Diana From jacobc <@t> mmc.org Mon Oct 2 09:29:40 2006 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Mon Oct 2 09:33:21 2006 Subject: [Histonet] Flame Dako.... Message-ID: I am also experiencing problems with Dako concentrate antibodies. They have apparently discontinued the EMA antibody I use and I had to wait over 2 months for the IgA-FITC antibody to be delivered. I see that there are no problems in Saudi Arabia receiving Dako products but for some reason Maine is off their radar! The biggest problem I am having with replacing the Dako products is finding IVD- or ASR-rated antibdies. I am anticipating CAP telling us we can't use them (I know they haven't yet, but I suspect they will soon). Therefore I don't really want to spend a bunch of time working up an RUO antibody. However, it is not easy to find repalcements with the appropriate rating. I have 2 Ventana XT's and I try to get as many antibodies from Ventana or Cell Marque as I can. BioGenex has mostly IVD-rated concentrates and offer a larger varitey of antibodies. I'm not completely stuck yet. Out of the 95 antibodies our lab offers, there is only one that I can only find an RUO for (Inhibin). I am afraid that with Dako limiting their antibody menu, I am going to run into more problems as I try to add more antibodies to our lab's request form! Maybe the manufacturers and vendors will take notice and do something before CAP does! Any comments?...... Chris Jacobs, HT(ASCP), QIHC NorDx Laboratory Scarborough, ME jacobc@mmc.org From victor <@t> pathology.washington.edu Mon Oct 2 10:19:49 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Oct 2 10:19:59 2006 Subject: [Histonet] Histology Position in Seattle - Update Message-ID: <45212E15.4050706@pathology.washington.edu> In my previous posting I listed the supervisor's email incorrectly. It is tanjie@u.washington.edu. Please contact Elaine for detailed information. Here is a link to the position. https://uwhires.admin.washington.edu/ENG/employees/default.cfm?szCategory=jobprofile&szOrderID=23959&szCandidateID=0&szSearchWords=pathology&szReturnToSearch=1 -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From gcallis <@t> montana.edu Mon Oct 2 10:25:31 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Oct 2 10:25:46 2006 Subject: [Histonet] The procedure that substitues xylene with mineral oil. In-Reply-To: <006a01c6e57e$513dc340$f300a8c0@ttth> References: <20061001165553.32345.qmail@web61219.mail.yahoo.com> <006a01c6e57e$513dc340$f300a8c0@ttth> Message-ID: <6.0.0.22.1.20061002091523.01b7a530@gemini.msu.montana.edu> Phil and Renee, If the solvents i.e. alcohols, xylene or xylene substitutes need to be collected for incineration after CJD cases, wouldn't mineral oil also need to be collected and disposed of in the same way? The expense of collection will still be there no matter what the powers that be do not like to do. Mineral oil may be less toxic compared to xylene but after contact.contamination with CJD or any other other prion diseased tissues, it's status changes back to being a collectable solvent. We work with prion diseased animal tissues using xylene substitutes (they work very well for processing and staining) and we are REQUIRED to collect these for incineration. At 11:23 AM 10/1/2006, you wrote: >I am attaching the procedure under separate cover. >Ren? J. >PhiHo Hoang wrote: >Greetings, >I am a new subscriber to this list and I am reading the list archive. >I came across a posting from Ren? J. responding to a request from Sharon: >Sharon: >I am sending privately a procedure I have that substitutes xylene with >mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. >Ren? J. >Sharon Allen <@t>exchange.hsc.mb.ca> wrote: >Does anyone use xylol substitutes, if so how good are they? >We do CJD testing & "the powers that be" don't like to pay for getting rid >of contaminated xylol. I don't want to have to start testing different >substitutes, so any help would be greatly appreciated. >Thanks >Sharon >sallen <@t> hsc.mb.ca >I am interested in this procedure >It is very much appreciated if I can get a copy >of that procedure. >Best regards, >PhiHo > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _____ > >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great >=39666/*http:/messenger.yahoo.com> rates starting at 1?/min. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From failm <@t> musc.edu Mon Oct 2 10:35:17 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Oct 2 10:35:48 2006 Subject: [Histonet] Commercial kits for checking glassware Message-ID: We use multi-check made by harleco Rena Fail From Charlene.Henry <@t> STJUDE.ORG Mon Oct 2 10:40:43 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Oct 2 10:40:58 2006 Subject: [Histonet] FW: Ad for ARC Histology Supervisor Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1E1B@SJMEMXMB02.stjude.sjcrh.local> We have a Histology Supervisor position in our veterinary Histology Lab just in case anyone is interested. St Jude Children's Research Hospital is a great place to work. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 > Job Title: Histology Supervisor, Requisition # 15512 > Job Location: Memphis, TN > > The Histology Supervisor in the Animal Resources Center supervises and > effectively manages staff of the Veterinary Histology laboratory. > Supervises and performs laboratory procedures and the interpretation > and reporting of results. Assists with the evaluation and testing of > new procedures and/or instrumentation; establishes maintenance > protocols. Ensures accurate records and quality control procedures > are implemented and maintained as required by the hospital and > accrediting, licensing/regulatory agencies (e.g. State of Tennessee, > AAALAC, and USDA). Coordinates the laboratory budget. > > The successful candidate will have the following requirements: > A high school diploma or GED is required. A Bachelor's degree or > equivalent (in concordance with current Federal and State Regulations) > is preferred. Additionally, six (6) years of experience as a > Histotechnologist or Histotechnician is required. Veterinary > histology experience is preferred. Supervisory experience is also > preferred. Certification as a Histotechnologist or Histotechnician by > the American Society of Clinical Pathology Board of Registry is > required. Subspecialty qualification in a related area (e.g. > Immunohistochemistry) is preferred. > > Qualified applicants may apply via our online process at > www.stjude.org/jobs. > > www.stjude.org > An Equal Opportunity Employer > > > > Kara M. Jackson > Research Recruiter > Human Resources Department > St. Jude Children's Research Hospital > 332 N. Lauderdale Street Mail Stop 507 > Memphis, Tennessee 38105-2794 > Office: 901-495-3259 > Fax: 901-495-3123 > email: kara.jackson@stjude.org > > From rjbuesa <@t> yahoo.com Mon Oct 2 10:50:02 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 2 10:50:11 2006 Subject: [Histonet] The procedure that substitues xylene with mineral oil. In-Reply-To: <6.0.0.22.1.20061002091523.01b7a530@gemini.msu.montana.edu> Message-ID: <20061002155002.40260.qmail@web61215.mail.yahoo.com> Gayle: It is evident that you have not read the procedure! Used minaral oil (= paraffin oil = paraffin of low molecular weight) is MIXED with the used paraffin and together form a solid mass to be incinerated after cooling. By the way, it is Ren? (male, just one "e") Ren? J. Gayle Callis wrote: Phil and Renee, If the solvents i.e. alcohols, xylene or xylene substitutes need to be collected for incineration after CJD cases, wouldn't mineral oil also need to be collected and disposed of in the same way? The expense of collection will still be there no matter what the powers that be do not like to do. Mineral oil may be less toxic compared to xylene but after contact.contamination with CJD or any other other prion diseased tissues, it's status changes back to being a collectable solvent. We work with prion diseased animal tissues using xylene substitutes (they work very well for processing and staining) and we are REQUIRED to collect these for incineration. At 11:23 AM 10/1/2006, you wrote: >I am attaching the procedure under separate cover. >Ren? J. >PhiHo Hoang wrote: >Greetings, >I am a new subscriber to this list and I am reading the list archive. >I came across a posting from Ren? J. responding to a request from Sharon: >Sharon: >I am sending privately a procedure I have that substitutes xylene with >mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. >Ren? J. >Sharon Allen <@t>exchange.hsc.mb.ca> wrote: >Does anyone use xylol substitutes, if so how good are they? >We do CJD testing & "the powers that be" don't like to pay for getting rid >of contaminated xylol. I don't want to have to start testing different >substitutes, so any help would be greatly appreciated. >Thanks >Sharon >sallen <@t> hsc.mb.ca >I am interested in this procedure >It is very much appreciated if I can get a copy >of that procedure. >Best regards, >PhiHo > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _____ > >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great >>=39666/*http:/messenger.yahoo.com> rates starting at 1?/min. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From gu.lang <@t> gmx.at Mon Oct 2 11:06:15 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Oct 2 11:06:16 2006 Subject: WG: [Histonet] Need major help with In Situ . . . Message-ID: <000601c6e63c$b08ee9a0$eeeea8c0@dielangs.at> _____ Von: Ion Beldorth [mailto:beldorth.msu@gmail.com] Gesendet: Montag, 02. Oktober 2006 15:06 An: gu.lang@gmx.at Betreff: Re: [Histonet] Need major help with In Situ . . . Gudrun, If you are referring to the 4%Paraformaldehyde followed by Methanol, I am not sure why it was done this way. My lab seems to always fix in Paraformaldehyde, but the methanol treatment is a new one, and I have heard it wasn't the best of ideas. I have suspected that freezing them in methanol simply 'removed' the stickiness from the tissue since reading in the Histonet archives that the fixation method can greatly affect adhesion. Anyone? Thoughts? Ion On 9/30/06, Gudrun Lang wrote: I have no answer for your problem, but I'm curious, why the embryos must be fixed before freezing and cutting. I have in my mind that fixed, frozen tissue sticks not really well on the slides. I remember darkly, that cryosections of fixed tissue were stained free floating in small dishes, and at last were mounted on a slide, what wasn't really easy. If your procedure first worked with the Superfrost slides, perhaps their shelflife is over? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto: histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von I.B. Gesendet: Samstag, 30. September 2006 01:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Need major help with In Situ . . . Hi All, I am working on a project involving embryonic Sea Lamprey and am having many difficulties with In Situ. Namely, the tissue sections (frozen cryo-sections) are washing off the slides (mostly during the 68oC, 2x and 0.1x SSC washes, but anything that is left washes off before they are coverslipped), all of them, 100% loss. Everything worked fine at first, but then I started losing tissue and have not been able to isolate the problem and develop a cure. The embryos were killed, fixed overnight in 4% Paraformaldehyde, then placed in absolute methanol at -80oC. Before use I bring them to water (100%, 95%, 70% ethanol, diH20, 2x 5mins each) then soak overnight in 20% Sucrose, then soak 4hrs-overnight in OCT compound before freezing. After sectioning I dry overnight at 40oC before performing ISH. I started using Fisher SuperFrost Plus and have tried APES treated slides (2% APES in acetone for 15min, wash in acetone, wash in diH2O, dried overnight at 50oC) with no luck. I have tired working with them laying flat (the slides, not me) rather than using vertically-situated staining jars, and using a barrier pen instead of gasketed coverwells to hold solutions (the suction created while removing the coverwells pulls sections off the slide). The latest attempt involved staining/counterstaining the entire embryo first, then cryo-sectioning and air drying the slides overnight, then clarifying and mounting. This worked great, lots of tissue on the slides, buuuutttt . . .the counterstain (Nuclear Fast Red) washed out while soaking overnight in 20% Sucrose prior to freezing. In addition the stain (NBT/BCIP) looks, well, not good. Smeared, or spilled (as in, 'spilled out' of the cells) may be a way to describe it. So in summary: Procedure: In Situ Hybridization DIG/Alkaline Phosphatase labeled RNA probes NBT/BCIP stain Nuclear Fast Red Counterstain Vectamount Permanent mounting media Problem: tissue falling off slides I am at a complete loss. Has anyone out there in Histoland worked with similar tissues or had a similar problem, and how did you fix it? I have heard of using RNase A after Hybridization in place of high-temp, high stringency SSC washes, but have not tired it (we do a lot of work with RNA in my lab, and I am a bit worried about working with RNase). Can anyone recommend this procedure? Hell, can anyone recommend ANYTHING? I do not know how to proceed from here, and your help is dearly appreciated. IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Oct 2 11:24:12 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Oct 2 11:24:22 2006 Subject: [Histonet] mineral oil, paraffin, xylene substitutes and xylene Message-ID: <6.0.0.22.1.20061002100356.01b04118@gemini.msu.montana.edu> Rene, Actually, I have read the publication in J of Histotechnology. However, the EXPENSE of collecting and incineration CJD contaminated material remains the same regardless of being a solid mass or liquid disposal. The point is the EXPENSE "the powers that be" are trying to avoid with contaminated xylene. At least, that is how I read Phil's initial inquiry. Unfortunately, this expense remains no matter what they use to replace xylene although consolidation of paraffin and the oil may reduce the overall amount to be carried away. They need to be prepared to pay for hauling it away. If we are going to be picky about spelling, "minaral" was just a typing error, correct? Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 Gayle: It is evident that you have not read the procedure! Used minaral oil (= paraffin oil = paraffin of low molecular weight) is MIXED with the used paraffin and together form a solid mass to be incinerated after cooling. By the way, it is Ren? (male, just one "e") Ren? J. Gayle Callis wrote: Phil and Renee, If the solvents i.e. alcohols, xylene or xylene substitutes need to be collected for incineration after CJD cases, wouldn't mineral oil also need to be collected and disposed of in the same way? The expense of collection will still be there no matter what the powers that be do not like to do. Mineral oil may be less toxic compared to xylene but after contact.contamination with CJD or any other other prion diseased tissues, it's status changes back to being a collectable solvent. We work with prion diseased animal tissues using xylene substitutes (they work very well for processing and staining) and we are REQUIRED to collect these for incineration. From rjbuesa <@t> yahoo.com Mon Oct 2 11:51:44 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 2 11:51:48 2006 Subject: [Histonet] mineral oil, paraffin, xylene substitutes and xylene In-Reply-To: <6.0.0.22.1.20061002100356.01b04118@gemini.msu.montana.edu> Message-ID: <20061002165144.26143.qmail@web61216.mail.yahoo.com> Gayle: Granted about the expense but if you work in a large hospital (like the one I was working in when I developed the procedure) there is not that much expense added because we incinerated our own materials and adding mineral oil to the paraffin was nothing of consideration. Another thing is incinerating a flammable substance like alcohols or xylene or its substitutes because that cannot be done readily in most facilities, although in this sense I was able also to convince the service department to mix the fuel they used to run the incinerator with used alcohols and xylene and at the end we had to dispose (using an outside company) of only formalin. So the expense issue is more relative to the size of the facility than to the procedure itself. Ren? J. Gayle Callis wrote: Rene, Actually, I have read the publication in J of Histotechnology. However, the EXPENSE of collecting and incineration CJD contaminated material remains the same regardless of being a solid mass or liquid disposal. The point is the EXPENSE "the powers that be" are trying to avoid with contaminated xylene. At least, that is how I read Phil's initial inquiry. Unfortunately, this expense remains no matter what they use to replace xylene although consolidation of paraffin and the oil may reduce the overall amount to be carried away. They need to be prepared to pay for hauling it away. If we are going to be picky about spelling, "minaral" was just a typing error, correct? Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 Gayle: It is evident that you have not read the procedure! Used minaral oil (= paraffin oil = paraffin of low molecular weight) is MIXED with the used paraffin and together form a solid mass to be incinerated after cooling. By the way, it is Ren? (male, just one "e") Ren? J. Gayle Callis wrote: Phil and Renee, If the solvents i.e. alcohols, xylene or xylene substitutes need to be collected for incineration after CJD cases, wouldn't mineral oil also need to be collected and disposed of in the same way? The expense of collection will still be there no matter what the powers that be do not like to do. Mineral oil may be less toxic compared to xylene but after contact.contamination with CJD or any other other prion diseased tissues, it's status changes back to being a collectable solvent. We work with prion diseased animal tissues using xylene substitutes (they work very well for processing and staining) and we are REQUIRED to collect these for incineration. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From Vfierke <@t> SNBLUSA.com Mon Oct 2 12:04:02 2006 From: Vfierke <@t> SNBLUSA.com (Vaughn Fierke) Date: Mon Oct 2 12:04:12 2006 Subject: [Histonet] isotype definition Message-ID: <3CB1E2E5EC8E9C48A06C8E0967EB82B2A10E4C@MAIL01.snblusa.com> Define isotype and use. Secondly, please explain the theory and need to run a negative isotype control? If you do get staining, what exactly is this telling you, everything else in your system being appropriate? I am specifically trying to set up a procedure for validating anibodies. Good references appreciated. Thank you. Confidentiality Notice: This letter, its contents and attachments are confidential and may contain privileged information. It is intended soley for the use of addressee(s) only. Any use, copying or disclosure of this communication or attachments to any other person is expressly prohibited without written permission of SNBL USA, Ltd. If you receive this message in error, please notify the sender at SNBL USA, Ltd immediately by return e-mail, telephone +1 425 407 0121, or fax +1 425 407 8601. We appreciate your cooperation. From Karen.Heckford <@t> CHW.edu Mon Oct 2 12:26:53 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Mon Oct 2 12:27:21 2006 Subject: [Histonet] CPT for H&E Message-ID: Happy Monday, The big wigs here have changed our productivity and the way of calculating it on us. I am trying to find procedures that are not getting into the system. We have the older Version of Powerpath. I noticed this morning that the H&E stains do not have a CPT code neither does H&E levels of their own.. I am not sure but I thought that the H&E should have a CPT code since we do this lab order on all of our tissue specimens or does it fall on under the CPT for that particular specimen. This should really bring up our productivity if it is individual. Please let me know how other hospitals are doing this? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Jerry <@t> ralambusa.com Mon Oct 2 12:29:00 2006 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Mon Oct 2 12:29:10 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 2 Message-ID: <3855F92002259948A66A8CA2D16E3A4F0177DC@server.ralambusa.com> Raymond A. Lamb, UK; based in the UK would have many alternatives to choose from. Please feel free to contact Dr. Neil Haine (neil@ralamb.com) or see www.ralamb.com. I hope that helps! Have a great day! Jerry Helisek? VP - North America 5409 Lumley Road, Unit #102 Durham, North Carolina 27703 Phone: 919-957-1964 Fax: 919-957-1972 Cell: 919-264-7964 jerry@ralambusa.com www.ralamb.com ? The information contained in this e-mail message may be privileged, confidential and protected from disclosure. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you think that you have received this e-mail message in error please e-mail thesender and delete the message. Thank you. Message: 5 Date: Mon, 2 Oct 2006 10:40:29 +0100 From: "Finlay Finlay" Subject: [Histonet] cassette pens To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Histonetters, This posting is for everyone who uses pens to mark histology cassettes. When we tried to replace our favourite Statmark pens, we discovered that we had to order them through Fisher in USA ( we are in Glasgow, Scotland) and would have to wait for weeks for the order to come through. In the meantime we ordered Secureline from VWR and they faded very badly! Good old fashioned pencil would have been better. Searching Histonet archives I found many complaints about these pens, and decided to complain to the manufacturer , Precision Dynamics. Their international sales coordinator , Ariane Bras has passed my comments on , but I wondered if anyone else who has had problems with these markers would send their views to her [abras@pdc-europe.com] maybe if enough people complain, they will make pens that are fit for purpose. We have now got our Statmark delivery from Fisher, it came through quite quickly, But does anyone know where to buy these in the UK? From SHARON.OSBORN <@t> SPCORP.COM Mon Oct 2 12:30:37 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Mon Oct 2 12:30:45 2006 Subject: [Histonet] Slide and cassette writers/printers Message-ID: <9A919A5D70313A4D9C56A02571087408C544E6@kenmsg40.us.schp.com> Alan, Sorry, I have been out for the past week or would have replied to this issue more quickly. Yes, do test out the various companies' products. I highly recommend the Leica Cassette and Slide printers. They are workhorses! We print up to several hundred slides a day on the slide printer. Sometimes, the cassette printer does a few hundred cassettes in a day. When there are problems, Leica service is very responsive. In addition, Leica has listened to the users and did a complete update of the product with a redesigned (much better and sturdier) unit. Then, they replaced the existing ones at no charge to the current users! Now, when you purchase their units, you get the best on the market for it has been "test driven" n both Europe and the USA so that you have a proven model ow. Leica did develop this in cooperation with another histology vendor. However, I do not know if the other vendor did all the updating and redesign on the original model that Leica has. Yes, there was a problem of it jamming on the first units; now, there are no problems with jamming slides. The units also are more quiet now than the first models. I do strongly encourage you to do the field testing, etc. before making a commitment. Again, we are very pleased with the Leica printers! Sharon Osborn DNAX, SP BioPharma Palo Alto, CA [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Bishop Sent: Wednesday, September 27, 2006 9:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette writers Anyone got any recomendation for cassette writers? Need to go down that route by the end of the year but don't really know what is on offer at the moment and what is good or bad! All input appreciated. Cheers Alan Bishop Charge Scientist Histopathology Medlab Central Palmerston North New Zealand ________________ ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From tbraud <@t> holyredeemer.com Mon Oct 2 13:12:21 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Oct 2 13:13:57 2006 Subject: [Histonet] CPT for H&E Message-ID: Welcome the "big-wigs" to the wonderful world of AP backwards billing, the part of the lab where the billing is done on the back end, by diagnosis and REPORTED stain interpretations. All H&Es are covered under the CPT code for the gross and microscopic for that specimen (which can also change depending on the diagnosis) REGARDLESS of the number of blocks submitted, or the number of H&Es stained. Ain't that a kick in the pants?!?!?! The bottom line is that billed tests have very little to do with productivity or workload in the Histo lab, tsk, tsk! Especially if you have a pathologist that insists on putting 80 blocks in on a uterus, where others might make do with 6. And that colon that was removed for diverticulitis that you just billed as a 88307??? Better make sure a tumor doesn't show up on the microscopic, because then you will be underbilling. Find out that the pathologist didn't need that Mucin after all? or worse, forgot to include the results in the final report? Then you can't charge for it! If its any consolation, cytology billing is much much worse! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, October 02, 2006 1:27 PM To: Histonet (E-mail) Subject: [Histonet] CPT for H&E Happy Monday, The big wigs here have changed our productivity and the way of calculating it on us. I am trying to find procedures that are not getting into the system. We have the older Version of Powerpath. I noticed this morning that the H&E stains do not have a CPT code neither does H&E levels of their own.. I am not sure but I thought that the H&E should have a CPT code since we do this lab order on all of our tissue specimens or does it fall on under the CPT for that particular specimen. This should really bring up our productivity if it is individual. Please let me know how other hospitals are doing this? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From Maxim_71 <@t> mail.ru Mon Oct 2 13:17:33 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Oct 2 13:18:58 2006 Subject: [Histonet] mineral oil, paraffin, xylene substitutes and xylene Message-ID: <566350156.20061002221733@mail.ru> Dear Gayle: In any solidify material any pathogenes moved much slovly, than in any liquids... This pertains to step of transportation waste rather then processing. Maxim Peshkov, histotechnologist Department of biopsy and cytological research Pathological and anatomical bureau Taganrog Russia, From kkusser <@t> trudeauinstitute.org Mon Oct 2 13:41:11 2006 From: kkusser <@t> trudeauinstitute.org (Kim Kusser) Date: Mon Oct 2 13:41:40 2006 Subject: [Histonet] BSL precautions for Primate frozen sectioning? Message-ID: <45212500.9214.0024.0@trudeauinstitute.org> Hi, I need a little (or, more than likely, a lot) of advice. I do a lot of frozen sectioning on mouse tissues. Our facility is only mouse. A researcher wants to be able to section baboon (uninfected and unfixed...just OCT embedded) tissue. Our cryostats are BSL2 (lab coats and gloves). To the people out there in the know, how do you treat Primate tissue when it comes to frozen sectioning in Cryostats? Would this procedure be recommended to all lab personnel without knowledge of their immune status (i.e. immunocompromised staff)? Thanks you for any and all help. Kim Kusser From beldorth.msu+hist <@t> gmail.com Mon Oct 2 14:03:21 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Mon Oct 2 14:03:28 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: References: Message-ID: Sarah, Our cryostat is a Leica 1850. Cryosectioning has, unfortunately, been an area of contention between myself and the person who trained me; specifically the blade angle. My trainer insists that the angle should never been changed, mostly because it has produced good results for her. However, the tissue I am sectioning is quite different and that angle (negative two, oddly enough) produces sections that curl into a tight tube and are therefore quite useless. By playing with the angle I have found that 3-4 degrees produces uncurled sections (more or less, they are often still 'springy' ) that I can actually use and, for a while, everything worked fine. I realize this compresses the tissue more, but can it affect adhesion as well? I would like to think that my cryosectioning technique is good, but I am of course rather biased and am always open to new and better ways. The Leica user manual is more of an Installation manual, and requests for clarification from there website on several issues has gone unanswered. Can you suggest a good source for cryosection technique and other information? Beyond that, I will try the paintbrush technique. I always avoid touching the tissue directly out of concern for damaging it, but at this point it can not hurt. There seems to be much praise for gelatin-subbing. A fellow histonetter sent me a protocol earlier, but if you can send me your favorite protocol for comparison that would be great. Ion p.s. Thanks to everyone who has answered so far. Sorry I replied to everyone backchannel, I did not realize that Gmail was sending my messages to private addresses instead of the listserv. Opps. On 10/2/06, Pixley, Sarah (pixleysk) < PIXLEYSK@ucmail.uc.edu > wrote: > > Dear Ion: > > We have had similar luck with SuperFrost slides. We do brain and nose > tissue, from rats perfused with 4% paraformaldehyde, 15-20% sucrose, frozen > and cryosectioned. The tissues were not sticking to the slides during > immunostaining. I have two suggestions, based on our experience: First, we > did find that improving our sectioning significantly helped keep the > sections on the slides. So you may want to check your cryostat and make sure > it is cutting well (new blade or freshly sharpened, check knife angle, etc. > and check out the sections right after fixing on the slide). If the sections > do not stick down well immediately, or if they have air bubbles under them > which cause slight wrinkles, then they can lift off in the immunostaining > procedures. It turned out to be a combination of getting a new technician up > to speed on sectioning (darn that learning curve!) and learning some new > tricks for sectioning. Someone showed us that if you take a cold dry paint > brush and lightly tap down the sections immediately after putting cold > sections on a cold slide, then you can avoid air bubble problems and get the > sections to stick better. However, Second, we also just gave up on > SuperFrost and went back to gelatin-subbing regular slides. That really > helps, even if the sections are not cut well. If you need a subbing > protocol, let me know. > > Sincerely, > > Sarah Pixley, Ph.D. > Associate Professor > Dept. of Cell Biology, Neurobiology and Anatomy > University of Cincinnati College of Medicine > Vontz Center for Molecular Studies, #3112 > PO Box 670521 (3125 Eden Ave) > Cincinnati, OH 45267-0521 > Tel# (513)-558-6086 > Fax# (513)-558-4454 > sarah.pixley@uc.edu > From rjbuesa <@t> yahoo.com Mon Oct 2 14:20:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 2 14:20:07 2006 Subject: [Histonet] BSL precautions for Primate frozen sectioning? In-Reply-To: <45212500.9214.0024.0@trudeauinstitute.org> Message-ID: <20061002192001.78360.qmail@web61213.mail.yahoo.com> Kim: I strongly recommend you to try to find out EXACTLY the conditions of those baboons. Remember that human AIDS virus is a mutant from a chimpanzee AIDS like virus! You should not mess around with unknown things after all! Ren? J. Kim Kusser wrote: Hi, I need a little (or, more than likely, a lot) of advice. I do a lot of frozen sectioning on mouse tissues. Our facility is only mouse. A researcher wants to be able to section baboon (uninfected and unfixed...just OCT embedded) tissue. Our cryostats are BSL2 (lab coats and gloves). To the people out there in the know, how do you treat Primate tissue when it comes to frozen sectioning in Cryostats? Would this procedure be recommended to all lab personnel without knowledge of their immune status (i.e. immunocompromised staff)? Thanks you for any and all help. Kim Kusser _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From Maxim_71 <@t> mail.ru Mon Oct 2 14:25:12 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Oct 2 14:26:00 2006 Subject: [Histonet] mineral oil, paraffin, xylene substitutes and xylene In-Reply-To: <6.2.3.4.0.20061002132904.03c05888@ander093.email.umn.edu> References: <566350156.20061002221733@mail.ru> <6.2.3.4.0.20061002132904.03c05888@ander093.email.umn.edu> Message-ID: <13110409000.20061002232512@mail.ru> LuAnn: I agree, but safety for they do not decrease. Maxim Maxim Peshkov, histotechnologist Department of biopsy and cytological research Pathological and anatomical bureau Taganrog Russia, From anh2006 <@t> med.cornell.edu Mon Oct 2 14:43:33 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Oct 2 14:58:41 2006 Subject: [Histonet] BSL precautions for Primate frozen sectioning? In-Reply-To: <20061002192001.78360.qmail@web61213.mail.yahoo.com> References: <20061002192001.78360.qmail@web61213.mail.yahoo.com> Message-ID: Even so, wouldn't you expect to treat it just like human tissue which could potentially also be hepatitis, HIV infected ... aka with universal precautions? Aren't we supposed to consider all human tissue as having those diseases and worse even if patient history is known, per universal precautions? The major thing to consider I believe is that primates commonly are infected with a potentially deadly form of hepatitis if humans get infected. Maybe some of you guys from SNBL or the like can speak up? >Kim: > I strongly recommend you to try to find out >EXACTLY the conditions of those baboons. > Remember that human AIDS virus is a mutant >from a chimpanzee AIDS like virus! > You should not mess around with unknown things after all! > Ren? J. > >Kim Kusser wrote: > Hi, > >I need a little (or, more than likely, a lot) of advice. I do a lot of >frozen sectioning on mouse tissues. Our facility is only mouse. A >researcher wants to be able to section baboon (uninfected and >unfixed...just OCT embedded) tissue. Our cryostats are BSL2 (lab coats >and gloves). > >To the people out there in the know, how do you treat Primate tissue >when it comes to frozen sectioning in Cryostats? Would this procedure be >recommended to all lab personnel without knowledge of their immune >status (i.e. immunocompromised staff)? > >Thanks you for any and all help. > >Kim Kusser -- From mari.ann.mailhiot <@t> leica-microsystems.com Mon Oct 2 15:32:46 2006 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Mon Oct 2 15:33:00 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: Message-ID: Ion Can you tell me what type of tissue you are cutting and how thick you are cutting your specimen. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com I.B. To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Re: Superfrost slides-Histonet question 10/02/2006 02:03 PM Sarah, Our cryostat is a Leica 1850. Cryosectioning has, unfortunately, been an area of contention between myself and the person who trained me; specifically the blade angle. My trainer insists that the angle should never been changed, mostly because it has produced good results for her. However, the tissue I am sectioning is quite different and that angle (negative two, oddly enough) produces sections that curl into a tight tube and are therefore quite useless. By playing with the angle I have found that 3-4 degrees produces uncurled sections (more or less, they are often still 'springy' ) that I can actually use and, for a while, everything worked fine. I realize this compresses the tissue more, but can it affect adhesion as well? I would like to think that my cryosectioning technique is good, but I am of course rather biased and am always open to new and better ways. The Leica user manual is more of an Installation manual, and requests for clarification from there website on several issues has gone unanswered. Can you suggest a good source for cryosection technique and other information? Beyond that, I will try the paintbrush technique. I always avoid touching the tissue directly out of concern for damaging it, but at this point it can not hurt. There seems to be much praise for gelatin-subbing. A fellow histonetter sent me a protocol earlier, but if you can send me your favorite protocol for comparison that would be great. Ion p.s. Thanks to everyone who has answered so far. Sorry I replied to everyone backchannel, I did not realize that Gmail was sending my messages to private addresses instead of the listserv. Opps. On 10/2/06, Pixley, Sarah (pixleysk) < PIXLEYSK@ucmail.uc.edu > wrote: > > Dear Ion: > > We have had similar luck with SuperFrost slides. We do brain and nose > tissue, from rats perfused with 4% paraformaldehyde, 15-20% sucrose, frozen > and cryosectioned. The tissues were not sticking to the slides during > immunostaining. I have two suggestions, based on our experience: First, we > did find that improving our sectioning significantly helped keep the > sections on the slides. So you may want to check your cryostat and make sure > it is cutting well (new blade or freshly sharpened, check knife angle, etc. > and check out the sections right after fixing on the slide). If the sections > do not stick down well immediately, or if they have air bubbles under them > which cause slight wrinkles, then they can lift off in the immunostaining > procedures. It turned out to be a combination of getting a new technician up > to speed on sectioning (darn that learning curve!) and learning some new > tricks for sectioning. Someone showed us that if you take a cold dry paint > brush and lightly tap down the sections immediately after putting cold > sections on a cold slide, then you can avoid air bubble problems and get the > sections to stick better. However, Second, we also just gave up on > SuperFrost and went back to gelatin-subbing regular slides. That really > helps, even if the sections are not cut well. If you need a subbing > protocol, let me know. > > Sincerely, > > Sarah Pixley, Ph.D. > Associate Professor > Dept. of Cell Biology, Neurobiology and Anatomy > University of Cincinnati College of Medicine > Vontz Center for Molecular Studies, #3112 > PO Box 670521 (3125 Eden Ave) > Cincinnati, OH 45267-0521 > Tel# (513)-558-6086 > Fax# (513)-558-4454 > sarah.pixley@uc.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From phiho.hoang <@t> rogers.com Mon Oct 2 15:43:30 2006 From: phiho.hoang <@t> rogers.com (PhiHo Hoang) Date: Mon Oct 2 15:43:39 2006 Subject: [Histonet] Reichert Jung 1130 Biocut Microtome lubrications Message-ID: <0d4901c6e663$6bf1d740$c70c010a@PhiHo> Greetings, It is very much appreciated if some kind souls would give advices on lubricating oil for the moving barrel (?) where the specimen holder is attached and grease for the bevel gears at the back. Best regards, PhiHo From beldorth.msu+hist <@t> gmail.com Mon Oct 2 15:49:39 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Mon Oct 2 15:49:44 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: References: Message-ID: Mari Ann, The tissue is embronic Sea Lamprey, 5-7mm long and 1mm thick at best, cut at -22oC in OCT compound (Tissue-Tec, Sukura Fintech Inc, I believe) to 10um. I have tried 12 and 14um with similar results. Ion On 10/2/06, mari.ann.mailhiot@leica-microsystems.com < mari.ann.mailhiot@leica-microsystems.com> wrote: > > Ion > > Can you tell me what type of tissue you are cutting and how thick you are > cutting your specimen. > > Best Regards > > > Mari Ann Mailhiot BA HT ASCP > Application Specialist > Leica Technical Assistance Center > 800 248 0123 x7267 > 847 236 3063 fax > mari.ann.mailhiot@leica-microsystems.com > www.leica-microsystems.com > > > > I.B. > t@gmail.com> To > Sent by: histonet@lists.utsouthwestern.edu > histonet-bounces@ cc > lists.utsouthwest > ern.edu Subject > [Histonet] Re: Superfrost > slides-Histonet question > 10/02/2006 02:03 > PM > > > > > > > > Sarah, > > Our cryostat is a Leica 1850. Cryosectioning has, unfortunately, been an > area of contention between myself and the person who trained me; > specifically the blade angle. My trainer insists that the angle should > never been changed, mostly because it has produced good results for her. > However, the tissue I am sectioning is quite different and that angle > (negative two, oddly enough) produces sections that curl into a tight tube > and are therefore quite useless. By playing with the angle I have found > that 3-4 degrees produces uncurled sections (more or less, they are often > still 'springy' ) that I can actually use and, for a while, everything > worked fine. I realize this compresses the tissue more, but can it affect > adhesion as well? I would like to think that my cryosectioning technique > is > good, but I am of course rather biased and am always open to new and > better > ways. The Leica user manual is more of an Installation manual, and > requests > for clarification from there website on several issues has gone > unanswered. > Can you suggest a good source for cryosection technique and other > information? > Beyond that, I will try the paintbrush technique. I always avoid touching > the tissue directly out of concern for damaging it, but at this point it > can > not hurt. > There seems to be much praise for gelatin-subbing. A fellow histonetter > sent me a protocol earlier, but if you can send me your favorite protocol > for comparison that would be great. > > Ion > > p.s. Thanks to everyone who has answered so far. Sorry I replied to > everyone backchannel, I did not realize that Gmail was sending my messages > to private addresses instead of the listserv. Opps. > > > On 10/2/06, Pixley, Sarah (pixleysk) < PIXLEYSK@ucmail.uc.edu > wrote: > > > > Dear Ion: > > > > We have had similar luck with SuperFrost slides. We do brain and nose > > tissue, from rats perfused with 4% paraformaldehyde, 15-20% sucrose, > frozen > > and cryosectioned. The tissues were not sticking to the slides during > > immunostaining. I have two suggestions, based on our experience: First, > we > > did find that improving our sectioning significantly helped keep the > > sections on the slides. So you may want to check your cryostat and make > sure > > it is cutting well (new blade or freshly sharpened, check knife angle, > etc. > > and check out the sections right after fixing on the slide). If the > sections > > do not stick down well immediately, or if they have air bubbles under > them > > which cause slight wrinkles, then they can lift off in the > immunostaining > > procedures. It turned out to be a combination of getting a new > technician > up > > to speed on sectioning (darn that learning curve!) and learning some new > > tricks for sectioning. Someone showed us that if you take a cold dry > paint > > brush and lightly tap down the sections immediately after putting cold > > sections on a cold slide, then you can avoid air bubble problems and get > the > > sections to stick better. However, Second, we also just gave up on > > SuperFrost and went back to gelatin-subbing regular slides. That really > > helps, even if the sections are not cut well. If you need a subbing > > protocol, let me know. > > > > Sincerely, > > > > Sarah Pixley, Ph.D. > > Associate Professor > > Dept. of Cell Biology, Neurobiology and Anatomy > > University of Cincinnati College of Medicine > > Vontz Center for Molecular Studies, #3112 > > PO Box 670521 (3125 Eden Ave) > > Cincinnati, OH 45267-0521 > > Tel# (513)-558-6086 > > Fax# (513)-558-4454 > > sarah.pixley@uc.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > From beldorth.msu+hist <@t> gmail.com Mon Oct 2 15:52:32 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Mon Oct 2 15:52:37 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: <6.0.0.22.1.20061002133323.01b18d30@gemini.msu.montana.edu> References: <6.0.0.22.1.20061002133323.01b18d30@gemini.msu.montana.edu> Message-ID: Thanks Gayle, I have actually been to Pathology Innovations and tried the brush technique, and done correctly it does prevent tissue from rolling, at least as the front edge comes off the block. Often, however, the tissue will roll from the back edge once it detaches. Not always, but the nature of the tissue (really thin) means that every section counts. I may start trying it again, however. Also, you kinda lost me on the angles bit. I counted 6 numbers and none of them seem to add up :) We use a low profile 'razor' blade and I take the 0deg mark to be 0deg. I set it to 3-4deg and 10um sections come off flat (provided, of course, that the anti-roll plate is positioned correctly). Well, actually sometimes a bit wavy, but at least not curled into a tube. More or less angle and they curl. It sounds like you get good results with a greater angle, and others have called for less. This seems to be one of those indeterminate situations that depends more on what works in a given setting for a specific tissue than what is correct or incorrect. Gotta love science . . . Ion On 10/2/06, Gayle Callis wrote: > > Ion, > > We have three 1850's and it is possible blade angles can be a bit > different > for thicker sections, the different types of blades one uses (high versus > low profile) and also differences between different blade > manufacturers. Compression generally is NOT good, as it is hard to get > the > section flat once it is compressed and then have it be flat on a > slide. When doing the brush technic, use a sable brush #1 or #2, and make > sure the tissue is surrounded by some OCT so you can grasp the frozen OCT > gently instead of the tissue. It is possible to use brush on unembedded > tissues but you can't be a Picasso (actually brushing/touching the tissue) > but rather let a few bristles guide the section onto the knife holder. Go > to Pathology Innovations website and watch Dr. Peters use the brush > technic, he does it as well as anyone around. I haven't used an antiroll > device for years with exception of 50 um prefixed tongue - sometimes I > have > to use it. We set our blade angle just one mark or 6 towards the 10 mark, > 5 being the middle. This would be an 11 to 12 degree angle. 5 to us > means 10 degrees, and I must admit, angles are sometimes hard to > understand > from one cryostat to the next. > > Your Leica representatives, often locally or even experts in their main > office, can be of help too. > mari.ann.mailhiot@leica-microsystems.com or > jan.minshew@leica-microsystems.com are two ladies who will steer you in > the > right direction, both are experts at microtomy/cryomicrotomy and sell > Leica > 1850's. > > Good luck on your dilemma > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > From ganesett <@t> fmed.edu.uy Mon Oct 2 17:50:39 2006 From: ganesett <@t> fmed.edu.uy (Gabriel Anesetti) Date: Mon Oct 2 16:41:49 2006 Subject: [Histonet] Using lectin BS-1 for staining endothelial cells Message-ID: <452197BF.8060402@fmed.edu.uy> Hello Histonetteers, I am having problems with the staining of rat endothelial cells in paraffin sections using a biotin labeled lectin from Bandeiraea Simplicifolia BS-1 (from Sigma). The lectin does not appear to be staining any of the endothelial cells. I have tried using citrate buffer for antigen retrieval and DAB, DAB + NiCl or FICT to visualize the site of reaction. Sections are from ovary grafts and have very new blood vessels forming. Any information would be helpful. Thanks Gabriel Anesetti Histology and Embryology Departament School of Medicine Montevideo, Uruguay ganesett@fmed.edu.uy From AGrobe2555 <@t> aol.com Mon Oct 2 17:02:55 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Oct 2 17:03:01 2006 Subject: [Histonet] BSL precautions for Primate frozen sectioning? Message-ID: <535.8704b98.3252e68f@aol.com> I believe it is a Simian Herpes virus that is deadly. Albert C. Grobe, PhD International Heart Institute, Tissue Engineering Lab From victor <@t> pathology.washington.edu Mon Oct 2 17:07:11 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Oct 2 17:08:05 2006 Subject: [Histonet] Leica Rep Message-ID: <45218D8F.3040705@pathology.washington.edu> Is there a rep for the Pacific NW that can provide pricing on the latest IPC? Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From beingmary53 <@t> sbcglobal.net Mon Oct 2 18:14:31 2006 From: beingmary53 <@t> sbcglobal.net (MARY JOHNSON) Date: Mon Oct 2 18:14:35 2006 Subject: [Histonet] Job opening Message-ID: <20061002231431.85346.qmail@web81607.mail.mud.yahoo.com> We have a full time Histo tech position at Harris county Medical Examinal Offical in Houston Tx, Please contact Dr. Wolf at 713-796-6829. From Jan.Minshew <@t> leica-microsystems.com Mon Oct 2 18:40:42 2006 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Mon Oct 2 18:40:53 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: Message-ID: Hello Ion, I see that Gayle Callis supplied you with my name and Mari Ann Mailhiot's as contacts at Leica who could assist you with your frozen section application questions. I just want to let you know that we will be happy to help in any way possible. Mari Ann is part of our Technical Assistance Center at Leica and spends a great deal of her time helping customers customize their applications and improve their results. Although your application of sectioning embryonic Sea Lamprey is rather unique, I'm certain that our (way too many) years of histology lab experience will be of some use in determining a solution to your problem. If you have any other questions or need additional help in the future, my signature provides the necessary information for you to contact the US division of Leica Microsystems. Best wishes, Jan Minshew, HT(ASCP)HTL Marketing Manager Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 I.B. To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Re: Superfrost slides-Histonet question 10/02/2006 02:03 PM Sarah, Our cryostat is a Leica 1850. Cryosectioning has, unfortunately, been an area of contention between myself and the person who trained me; specifically the blade angle. My trainer insists that the angle should never been changed, mostly because it has produced good results for her. However, the tissue I am sectioning is quite different and that angle (negative two, oddly enough) produces sections that curl into a tight tube and are therefore quite useless. By playing with the angle I have found that 3-4 degrees produces uncurled sections (more or less, they are often still 'springy' ) that I can actually use and, for a while, everything worked fine. I realize this compresses the tissue more, but can it affect adhesion as well? I would like to think that my cryosectioning technique is good, but I am of course rather biased and am always open to new and better ways. The Leica user manual is more of an Installation manual, and requests for clarification from there website on several issues has gone unanswered. Can you suggest a good source for cryosection technique and other information? Beyond that, I will try the paintbrush technique. I always avoid touching the tissue directly out of concern for damaging it, but at this point it can not hurt. There seems to be much praise for gelatin-subbing. A fellow histonetter sent me a protocol earlier, but if you can send me your favorite protocol for comparison that would be great. Ion p.s. Thanks to everyone who has answered so far. Sorry I replied to everyone backchannel, I did not realize that Gmail was sending my messages to private addresses instead of the listserv. Opps. On 10/2/06, Pixley, Sarah (pixleysk) < PIXLEYSK@ucmail.uc.edu > wrote: > > Dear Ion: > > We have had similar luck with SuperFrost slides. We do brain and nose > tissue, from rats perfused with 4% paraformaldehyde, 15-20% sucrose, frozen > and cryosectioned. The tissues were not sticking to the slides during > immunostaining. I have two suggestions, based on our experience: First, we > did find that improving our sectioning significantly helped keep the > sections on the slides. So you may want to check your cryostat and make sure > it is cutting well (new blade or freshly sharpened, check knife angle, etc. > and check out the sections right after fixing on the slide). If the sections > do not stick down well immediately, or if they have air bubbles under them > which cause slight wrinkles, then they can lift off in the immunostaining > procedures. It turned out to be a combination of getting a new technician up > to speed on sectioning (darn that learning curve!) and learning some new > tricks for sectioning. Someone showed us that if you take a cold dry paint > brush and lightly tap down the sections immediately after putting cold > sections on a cold slide, then you can avoid air bubble problems and get the > sections to stick better. However, Second, we also just gave up on > SuperFrost and went back to gelatin-subbing regular slides. That really > helps, even if the sections are not cut well. If you need a subbing > protocol, let me know. > > Sincerely, > > Sarah Pixley, Ph.D. > Associate Professor > Dept. of Cell Biology, Neurobiology and Anatomy > University of Cincinnati College of Medicine > Vontz Center for Molecular Studies, #3112 > PO Box 670521 (3125 Eden Ave) > Cincinnati, OH 45267-0521 > Tel# (513)-558-6086 > Fax# (513)-558-4454 > sarah.pixley@uc.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From yeepengtiang <@t> hotmail.com Mon Oct 2 19:06:02 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Mon Oct 2 19:06:11 2006 Subject: [Histonet] blocking peptide Message-ID: Dear all, I am currently using a cell-cycle marker, which is the Ki-67 made in the mouse for immunostaining. However, I need to do a western blot to validate my host protein in HeLa cells and I need a blocking peptide. The company which I bought my Ab from doesnt sell the peptide and I have to outsource from other companies. However, I found that the peptides are sold with the Ki-67 Ab made in other hosts and no one is actually selling blocking peptide for Ki-67 made in mouse. Will there be any problem if I use the peptide which is meant for Ki-67Ab made in other hosts (instead of mouse in which my Ab is made from)? Thanks in advance! With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. Correspondence address: Molecular Diagnostics Research Laboratory, (c/o: Clinical Diagnostics Laboratory) 4th floor, East Tower, University of Malaya Medical Centre, 50603 Pantai Valley, Kuala Lumpur, MALAYSIA. Tel: (+6)03-79492205 Fax: (+6)03-79492818 From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue Oct 3 04:30:26 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue Oct 3 04:33:58 2006 Subject: [Histonet] Statmark pens Message-ID: We get ours from Cellpath. The black ones are fine; however, when we were given a red one to try, the ink completely disappeared after processing. I repeated the test with the 2 colours and the same thing happened again! Having tested and used the black version for the last two years with no problems, we could have gone ahead and trusted the red pen on the routine blocks - good job we didn't ! Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From histobr <@t> yahoo.com Tue Oct 3 06:57:30 2006 From: histobr <@t> yahoo.com (Ruser Rebecca) Date: Tue Oct 3 06:57:39 2006 Subject: [Histonet] c4d antibody Message-ID: <20061003115730.57611.qmail@web56502.mail.re3.yahoo.com> Can anyone provide me with information on obtaining C4d antibody for use on paraffin sections? I found an article about an antibody from Biomedica but can't find where to purchase this antibody in the U.S. Thanks for any help you can give me. Rebecca Ruser --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From Don.Birgerson <@t> leica-microsystems.com Tue Oct 3 07:31:09 2006 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Tue Oct 3 07:31:24 2006 Subject: [Histonet] Reichert Jung 1130 Biocut Microtome lubrications In-Reply-To: <0d4901c6e663$6bf1d740$c70c010a@PhiHo> Message-ID: Hi PhiHo, The oil for the 1130 Biocut is #14033606081 Type 404 and the grease is 14033624744 Kluber Topas (color is white).These are available from Leica-Microsystems .Follow the lubrication guide "glued" inside the lid. If you have further questions, please feel free to phone.. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 7:00 - 4:00 CDT "PhiHo Hoang" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Reichert Jung 1130 Biocut Microtome lubrications 10/02/2006 03:43 PM Greetings, It is very much appreciated if some kind souls would give advices on lubricating oil for the moving barrel (?) where the specimen holder is attached and grease for the bevel gears at the back. Best regards, PhiHo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From phiho.hoang <@t> rogers.com Tue Oct 3 08:48:26 2006 From: phiho.hoang <@t> rogers.com (PhiHo Hoang) Date: Tue Oct 3 08:48:15 2006 Subject: [Histonet] Reichert Jung 1130 Biocut Microtome lubrications References: Message-ID: <0e2301c6e6f2$9a832d40$c70c010a@PhiHo> Hi Don, Thanks for the info. I will contact you off list for details. Cheers, PhiHo ----- Original Message ----- From: To: "PhiHo Hoang" Cc: ; Sent: Tuesday, October 03, 2006 8:31 AM Subject: Re: [Histonet] Reichert Jung 1130 Biocut Microtome lubrications > Hi PhiHo, > The oil for the 1130 Biocut is #14033606081 Type 404 and the grease > is 14033624744 Kluber Topas (color is white).These are available from > Leica-Microsystems .Follow the lubrication guide "glued" inside the lid. > If you have further questions, please feel free to phone.. > Best regards, > Don > > > > Don Birgerson > Leica Microsystems > Technical Assistance Center > Don.Birgerson@Leica-Microsystems.Com > 1-800-248-0123 ext 5918 7:00 - 4:00 CDT > > > > "PhiHo Hoang" > rs.com> To > Sent by: > histonet-bounces@ cc > lists.utsouthwest > ern.edu Subject > [Histonet] Reichert Jung 1130 > Biocut Microtome lubrications > 10/02/2006 03:43 > PM > > > > > > > > Greetings, > > It is very much appreciated if some kind souls would give > advices on lubricating oil for the moving barrel (?) where > the specimen holder is attached and grease for the bevel gears > at the back. > > Best regards, > > PhiHo > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ From bayoubelle311 <@t> gmail.com Tue Oct 3 09:22:05 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Tue Oct 3 09:22:15 2006 Subject: [Histonet] Re: Histonet Digest, Vol 35, Issue 3 In-Reply-To: <452259fe.0b850506.4336.ffff8baaSMTPIN_ADDED@mx.gmail.com> References: <452259fe.0b850506.4336.ffff8baaSMTPIN_ADDED@mx.gmail.com> Message-ID: <398f02c20610030722t2877b5d1y9bf0fbda94e94ac3@mail.gmail.com> Hi all... A colleague of mine is searching for the ICSH 1998 method for reticulocyte/RBC ratio... does anyone have a copy on hand? thanks Melissa From PMonfils <@t> Lifespan.org Tue Oct 3 09:39:18 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Oct 3 09:39:34 2006 Subject: [Histonet] Wanted: Used Embedding Unit/Embedding Center Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017177B2@lsexch.lsmaster.lifespan.org> Would you like to get rid of that old embedding unit that has been stored under the bench since you got your new one five years ago? I am not fussy about brand or model, as long as you can verify that it is working condition. An older unit is ok. (It's highly unlikely that you have one as old as the one we are getting rid of. We bought it reconditioned, 20 years ago.) If you have one you would like to sell, please email me and provide me with details and a price. Paul Monfils Core Research Laboratories Providence, Rhode Island From emerald_lake77 <@t> yahoo.com Tue Oct 3 11:29:26 2006 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Tue Oct 3 11:29:32 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution can be reversible? Message-ID: <20061003162926.90321.qmail@web31702.mail.mud.yahoo.com> Hello, I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax. Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this?? Also, what affect on antigenicity will such storage in PBS have on these samples? Thank you all so much for your help. G. H. Cambridge, MA Key words: NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From gu.lang <@t> gmx.at Tue Oct 3 11:32:09 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 3 11:32:11 2006 Subject: AW: [Histonet] c4d antibody In-Reply-To: <20061003115730.57611.qmail@web56502.mail.re3.yahoo.com> Message-ID: <000201c6e709$79a47220$eeeea8c0@dielangs.at> I found this: http://www.alpco.com/single.asp?CatNumber=04-BI-RC4D Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Ruser Rebecca Gesendet: Dienstag, 03. Oktober 2006 13:58 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] c4d antibody Can anyone provide me with information on obtaining C4d antibody for use on paraffin sections? I found an article about an antibody from Biomedica but can't find where to purchase this antibody in the U.S. Thanks for any help you can give me. Rebecca Ruser --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Oct 3 11:48:30 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Oct 3 11:48:36 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? Message-ID: This is really opening a barrel of worms but... Re formaldehyde solutions. If tissues are fixed for a few hours in standard formalin solutions then it is possible to reverse the fixation process by washing. It is a reversible process in that the bonds that have been formed are generally temporary and can be broken. Technically if you wish for "adequate or true fixation" then Pearse provides the theory for why this should be for about 1 week. (The fixation process does in fact continue for years). Of course no one fixes for one week except by accident. However the fixation for 24 to 48 hours is only an initial fixation. Fixation is generally carried on by the alcohols used for the processing. A. G. E. Pearse "Histochemistry Theoretical and Applied: volume 1 Preparative and Optical Technology." Churchill Livingstone. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Tuesday, October 03, 2006 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? Hello, I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax. Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this?? Also, what affect on antigenicity will such storage in PBS have on these samples? Thank you all so much for your help. G. H. Cambridge, MA Key words: NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 3 12:04:39 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 3 12:04:44 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution can be reversible? In-Reply-To: <20061003162926.90321.qmail@web31702.mail.mud.yahoo.com> Message-ID: <20061003170439.44792.qmail@web61211.mail.yahoo.com> GTH: A piece of tissue sitting in PBS for 1 month after being fixed for 24-48 hours only is likely to have suffered tissue decomposition at the center of it, specially if it was a thick piece. Formalin is an extremely fast fixative but with a very slow rate of penetration, this would have determined external fixation but not complete fixation. Fixation is not really reversible in a strict way and that is why you need a strong Heat Inducet Epitope Retrieval (HIER) before IHC to "undo" or "unfix" the crosslinkage produced by formalin. The damage to the tissue could have been caused by improper storage = PBS at room temperature during 1 month. If processed that tissue is very likely to present altered microscopic appearance. Just my opinion! Ren? J. GT Hebert wrote: Hello, I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax. Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this?? Also, what affect on antigenicity will such storage in PBS have on these samples? Thank you all so much for your help. G. H. Cambridge, MA Key words: NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From bhewlett <@t> cogeco.ca Tue Oct 3 12:20:15 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Oct 3 12:20:23 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution canbereversible? References: Message-ID: <006b01c6e710$31ba6530$6500a8c0@mainbox> Barry is quite correct. Further; Even after fixation times of longer than one week, formaldehyde fixation can still be partially reversed. This is, after all, why HIER works so well. Following fixation for 24-48 hours, sitting in PBS for a month will certainly have largely reversed the initial fixation. There is evidence that following 24 hour fixation in formaldehyde a water wash for 6 days will reverse 90% of formaldehyde binding. (see reference). The tissues will have been largely re-fixed in the processing alcohols. The effect of this will depend on the antigen of interest. It may be beneficial (antigenicity restored) or detrimental. The following reference may be of use; Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. 1994; 69, 177-179. Bryan ----- Original Message ----- From: "Rittman, Barry R" To: "GT Hebert" ; Sent: Tuesday, October 03, 2006 12:48 PM Subject: RE: [Histonet] Proof that fixation in formaldehyde solution canbereversible? This is really opening a barrel of worms but... Re formaldehyde solutions. If tissues are fixed for a few hours in standard formalin solutions then it is possible to reverse the fixation process by washing. It is a reversible process in that the bonds that have been formed are generally temporary and can be broken. Technically if you wish for "adequate or true fixation" then Pearse provides the theory for why this should be for about 1 week. (The fixation process does in fact continue for years). Of course no one fixes for one week except by accident. However the fixation for 24 to 48 hours is only an initial fixation. Fixation is generally carried on by the alcohols used for the processing. A. G. E. Pearse "Histochemistry Theoretical and Applied: volume 1 Preparative and Optical Technology." Churchill Livingstone. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Tuesday, October 03, 2006 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? Hello, I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax. Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this?? Also, what affect on antigenicity will such storage in PBS have on these samples? Thank you all so much for your help. G. H. Cambridge, MA Key words: NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Oct 3 12:28:26 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 3 12:28:23 2006 Subject: AW: [Histonet] Proof that fixation in formaldehyde solution can bereversible? In-Reply-To: <20061003170439.44792.qmail@web61211.mail.yahoo.com> Message-ID: <000301c6e711$55d80ac0$eeeea8c0@dielangs.at> Sorry Rene, but isn't it the other way round? That penetration is rather fast and fixation in the sense of making methylenbridges is rather slowly? So tissue of 5 mm is penetrated within a few hours, but fixation takes much longer? I also learned that fixation with formaldehyd can be reversed by washing in tapwater for several weeks. In some old books they recommend washing the tissue the same duration as fixation was done. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Dienstag, 03. Oktober 2006 19:05 An: GT Hebert; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Proof that fixation in formaldehyde solution can bereversible? GTH: A piece of tissue sitting in PBS for 1 month after being fixed for 24-48 hours only is likely to have suffered tissue decomposition at the center of it, specially if it was a thick piece. Formalin is an extremely fast fixative but with a very slow rate of penetration, this would have determined external fixation but not complete fixation. Fixation is not really reversible in a strict way and that is why you need a strong Heat Inducet Epitope Retrieval (HIER) before IHC to "undo" or "unfix" the crosslinkage produced by formalin. The damage to the tissue could have been caused by improper storage = PBS at room temperature during 1 month. If processed that tissue is very likely to present altered microscopic appearance. Just my opinion! Ren? J. GT Hebert wrote: Hello, I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax. Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this?? Also, what affect on antigenicity will such storage in PBS have on these samples? Thank you all so much for your help. G. H. Cambridge, MA Key words: NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rschoon <@t> email.unc.edu Tue Oct 3 12:35:00 2006 From: rschoon <@t> email.unc.edu (rschoon@email.unc.edu) Date: Tue Oct 3 12:34:13 2006 Subject: [Histonet] Amino Cupric Silver method Message-ID: <20061003133500.k0khdepy7ocs0ksk@webmail4.isis.unc.edu> Can anyone out there give me a refferance to a Amino Cupric Silver method (ACS). TIA Robert Schoonhoven From bhewlett <@t> cogeco.ca Tue Oct 3 12:41:18 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Oct 3 12:41:32 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? References: <20061003170439.44792.qmail@web61211.mail.yahoo.com> Message-ID: <007401c6e713$24c3bb80$6500a8c0@mainbox> Rene, You wrote; >>Formalin is an extremely fast fixative but with a very slow rate of >>penetration, ....<< Actually, the reverse is the case! Formaldehyde has very fast penetration(possibly the fastest that we use) with a diffusion coefficient(K) of at least 2.0 and most likely 3.5. Compared to alcohol with a diffusion coefficient(K) of 0.8-1.0. Penetration can be calculated from the following; d = K times the square root of time, where d = distance in mm and time is in hours. However, the real problem is fixation. This is very slow, taking at least 24 hours at RT for 90% of formaldehyde binding to occur. This is due to the 'clock' reaction exhibited by formaldehyde solutions. Once the binding has occurred , then more cross-linking can proceed. See; Fox CH et al. Formaldehyde fixation. J. Histochem. Cytochem. 1985; 33, 845-853. Bryan ----- Original Message ----- From: "Rene J Buesa" To: "GT Hebert" ; Sent: Tuesday, October 03, 2006 1:04 PM Subject: Re: [Histonet] Proof that fixation in formaldehyde solution can bereversible? > GTH: > A piece of tissue sitting in PBS for 1 month after being fixed for 24-48 > hours only is likely to have suffered tissue decomposition at the center > of it, specially if it was a thick piece. Formalin is an extremely fast > fixative but with a very slow rate of penetration, this would have > determined external fixation but not complete fixation. > Fixation is not really reversible in a strict way and that is why you > need a strong Heat Inducet Epitope Retrieval (HIER) before IHC to "undo" > or "unfix" the crosslinkage produced by formalin. > The damage to the tissue could have been caused by improper storage = PBS > at room temperature during 1 month. If processed that tissue is very > likely to present altered microscopic appearance. > Just my opinion! > Ren? J. > > GT Hebert wrote: > Hello, > > I am in a heated debate with an investigator regarding his samples. They > were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure > exactly which one) and then switch to PBS for shipment. They sat at room > temperature for over 1 month before being processed routinely and embedded > in paraffin wax. > > Can someone tell me if indeed the fixation is reversible, or once they > have been fixed for over 24hrs they remain fixed?? Can anyone refer me to > books or papers that talk about this?? > > Also, what affect on antigenicity will such storage in PBS have on these > samples? > > Thank you all so much for your help. > > G. H. > Cambridge, MA > > > > Key words: > NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, > reverse, reversible. > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates > starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Tue Oct 3 12:55:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 3 12:55:17 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? In-Reply-To: <007401c6e713$24c3bb80$6500a8c0@mainbox> Message-ID: <20061003175509.97990.qmail@web61218.mail.yahoo.com> Bryan: This is I think the "can of worms" that was mentioned before. If you put your fingers in formalin for just a few seconds you sill feel the numbness cause by the quick fixation and this same quick fixation is the one preventing the penetration with less and less penetration as more and more tissue is fixed. The coefficients of diffusability for major fixing agents (Bancroft & Stevens: Theory and practice of histological techniques", Table 2.1 based on reserach by Medawar) are as follows: potassium dichromate ---1.33 acetic acid ----1.2 ethanol---1.0 mercuric chloride--0.78 to 0.84 formaldehyde---0.78 That is why the combination of some of these reagents are able to take advantage of the quick fixation by formalin with the high penetration rates of the others. That is why to obtain a proper fixation with NBF it is required, not only at least 24 hours, but also why almost any processing protocol includes 1 or 2 initial station for a secondary fixation. Ren? J. Bryan Hewlett wrote: Rene, You wrote; >>Formalin is an extremely fast fixative but with a very slow rate of >>penetration, ....<< Actually, the reverse is the case! Formaldehyde has very fast penetration(possibly the fastest that we use) with a diffusion coefficient(K) of at least 2.0 and most likely 3.5. Compared to alcohol with a diffusion coefficient(K) of 0.8-1.0. Penetration can be calculated from the following; d = K times the square root of time, where d = distance in mm and time is in hours. However, the real problem is fixation. This is very slow, taking at least 24 hours at RT for 90% of formaldehyde binding to occur. This is due to the 'clock' reaction exhibited by formaldehyde solutions. Once the binding has occurred , then more cross-linking can proceed. See; Fox CH et al. Formaldehyde fixation. J. Histochem. Cytochem. 1985; 33, 845-853. Bryan ----- Original Message ----- From: "Rene J Buesa" To: "GT Hebert" ; Sent: Tuesday, October 03, 2006 1:04 PM Subject: Re: [Histonet] Proof that fixation in formaldehyde solution can bereversible? > GTH: > A piece of tissue sitting in PBS for 1 month after being fixed for 24-48 > hours only is likely to have suffered tissue decomposition at the center > of it, specially if it was a thick piece. Formalin is an extremely fast > fixative but with a very slow rate of penetration, this would have > determined external fixation but not complete fixation. > Fixation is not really reversible in a strict way and that is why you > need a strong Heat Inducet Epitope Retrieval (HIER) before IHC to "undo" > or "unfix" the crosslinkage produced by formalin. > The damage to the tissue could have been caused by improper storage = PBS > at room temperature during 1 month. If processed that tissue is very > likely to present altered microscopic appearance. > Just my opinion! > Ren? J. > > GT Hebert wrote: > Hello, > > I am in a heated debate with an investigator regarding his samples. They > were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure > exactly which one) and then switch to PBS for shipment. They sat at room > temperature for over 1 month before being processed routinely and embedded > in paraffin wax. > > Can someone tell me if indeed the fixation is reversible, or once they > have been fixed for over 24hrs they remain fixed?? Can anyone refer me to > books or papers that talk about this?? > > Also, what affect on antigenicity will such storage in PBS have on these > samples? > > Thank you all so much for your help. > > G. H. > Cambridge, MA > > > > Key words: > NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, > reverse, reversible. > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates > starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From beldorth.msu+hist <@t> gmail.com Tue Oct 3 12:56:01 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Tue Oct 3 12:56:08 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: <6.0.0.22.1.20061002155856.01b3ad78@gemini.msu.montana.edu> References: <6.0.0.22.1.20061002133323.01b18d30@gemini.msu.montana.edu> <6.0.0.22.1.20061002155856.01b3ad78@gemini.msu.montana.edu> Message-ID: Hi Gayle, Looks like I have to eat my earlier words. I was informed we use low profile blades, though it turns out they are Leica 818 high profile blades. Oh, if I could make a list of the things I was incorrectly taught . . . Anyway, exactly how slowly do you turn the wheel? You make it sound like a three-toed sloth made it's way into a biology lab :0 This seems to be another of those areas that depends on tissue and freezing media. I used to section adult Sea Lamprey brain & nasal epithelium surrounded by a good deal of additional tissue, and the cutting stroke was very fast, not quite violently fast, but quite fast. My embryos get mad at me if I treat them like that. Has anyone ever considered converting this list to a Web Forum? That way there can be some permanent posts stickied to the top dealing with issues that tend to send newbies into convulsions. I will play around with things some more, but I am pretty sure I am as cold and slow as I can get. Ion On 10/2/06, Gayle Callis wrote: > > Ion, > > I will go back and look at the blade holder again - angles will drive you > nuts!!! > > When I finish the cutting stroke i.e. turning the flywheel, I generally > stop near the top and NOT let the section release entirely from the block > face. I can maintain a flat section without curling this way, using the > brush. > > I do not use low profile blades, they are too flimsy for my work, and high > profiles are just as sharp, but sturdier, especially for thick or tougher > tissue sections. What blade are you using? > > We have use Tissue Tek Accuedge for years in our cryostats although I do > NOT use them for paraffin. I can't cut sucrose protected tissues at -22, > just too warm - but it may be the nature of the tissue used too and mine > is > much different than your lamprey! I crank it down to -25 or -26 for > anything prefixed, sucrose protected or the sucrose oozes out like syrup. > > I section VERY slowly which helps keep that section attached at top of > blade better. In fact, I section slower than most automated cryostats! > > Will let you know the angle numbers - > > Gayle (yeah, science is a hoot!) > > > > At 02:52 PM 10/2/2006, you wrote: > >Thanks Gayle, > > > >I have actually been to Pathology Innovations and tried the brush > technique, > >and done correctly it does prevent tissue from rolling, at least as the > >front edge comes off the block. Often, however, the tissue will roll > from > >the back edge once it detaches. Not always, but the nature of the tissue > >(really thin) means that every section counts. I may start trying it > again, > >however. Also, you kinda lost me on the angles bit. I counted 6 numbers > >and none of them seem to add up :) We use a low profile 'razor' blade > and I > >take the 0deg mark to be 0deg. I set it to 3-4deg and 10um sections come > >off flat (provided, of course, that the anti-roll plate is positioned > >correctly). Well, actually sometimes a bit wavy, but at least not curled > >into a tube. More or less angle and they curl. It sounds like you get > good > >results with a greater angle, and others have called for less. This > seems > >to be one of those indeterminate situations that depends more on what > works > >in a given setting for a specific tissue than what is correct or > incorrect. > >Gotta love science . . . > > > >Ion > > > > > > > > > >On 10/2/06, Gayle Callis wrote: > >> > >>Ion, > >> > >>We have three 1850's and it is possible blade angles can be a bit > >>different > >>for thicker sections, the different types of blades one uses (high > versus > >>low profile) and also differences between different blade > >>manufacturers. Compression generally is NOT good, as it is hard to get > >>the > >>section flat once it is compressed and then have it be flat on a > >>slide. When doing the brush technic, use a sable brush #1 or #2, and > make > >>sure the tissue is surrounded by some OCT so you can grasp the frozen > OCT > >>gently instead of the tissue. It is possible to use brush on unembedded > >>tissues but you can't be a Picasso (actually brushing/touching the > tissue) > >>but rather let a few bristles guide the section onto the knife > holder. Go > >>to Pathology Innovations website and watch Dr. Peters use the brush > >>technic, he does it as well as anyone around. I haven't used an > antiroll > >>device for years with exception of 50 um prefixed tongue - sometimes I > >>have > >>to use it. We set our blade angle just one mark or 6 towards the 10 > mark, > >>5 being the middle. This would be an 11 to 12 degree angle. 5 to us > >>means 10 degrees, and I must admit, angles are sometimes hard to > >>understand > >>from one cryostat to the next. > >> > >>Your Leica representatives, often locally or even experts in their main > >>office, can be of help too. > >> mari.ann.mailhiot@leica-microsystems.com or > >>jan.minshew@leica-microsystems.com are two ladies who will steer you in > >>the > >>right direction, both are experts at microtomy/cryomicrotomy and sell > >>Leica > >>1850's. > >> > >>Good luck on your dilemma > >> > >>Gayle Callis > >>MT,HT,HTL(ASCP) > >>Research Histopathology Supervisor > >>Veterinary Molecular Biology > >>Montana State University - Bozeman > >>PO Box 173610 > >>Bozeman MT 59717-3610 > >>406 994-6367 > >>406 994-4303 (FAX) > >> > >> > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From beldorth.msu+hist <@t> gmail.com Tue Oct 3 12:57:02 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Tue Oct 3 12:57:08 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: References: Message-ID: Mari Ann, Yes, quite soft tissue. Freezing took a while to figure out, but I now do it in much the same manner as the Paper Embedding technique at Pathology Innovations. The difficulty is in acting quickly enough for proper contact and freezing. I have tried freezing the tissue in 200 proof ethanol in Li N2, but apparently I am doing something wrong, as the tissue and OCT compound around it simply refuse to cut (even though it is allowed to come to cabinet temperature for eight hours before I attempt to section it). I am sure the error is on my end, I just haven't experimented enough with the technique to know where. Cutting at a colder temperature, at least with OCT, tends to fracture the OCT - it looks and sounds like I am cutting through a block of ice. I have tried a sample of Cryo-Gel from Instrumedics which seems to need a colder temperature for sectioning but, contrary to it's intended purpose, it is more difficult to work with. So it goes. I'll try playing with the blade angle some more (my trainer would have an aneurysm if she hear me say that), but what is the chance that will prevent tissue loss? This is great though; I would like to keep the sectioning conversation going (along with about half a dozen other issues), as searching though the Histonet achieves doesn't produce much helpful information. Cryo-sectioning, proper use of a cryostat, and what sections look like when you do things right/wrong, as well as how to remedy common problems, seems to be one of those grey areas where we are at the whim of whomever teaches us. When I have some more time, I will contact you and Jan about customizing my application. Ion From petepath <@t> yahoo.com Tue Oct 3 13:01:44 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Oct 3 13:01:50 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question Message-ID: <20061003180144.47575.qmail@web30406.mail.mud.yahoo.com> Hi Ion, In our Leica !850's I use a blade angle of 5 with our low profile blades. I know I would not have much luck at a negative angle. As far as curling tissue, in my experience the best temperature for cutting most tissus (except fat) is the warmest temp that the tissue will cut without crumpling. Try starting to cut a touch on the warm side. Press a piece of cryostat temp metal ( I use my over-chuck blocks for this) on the block face a few seconds at a time until the tissue starts to cut. When the tissue is just cold enough to cut it will float off in a flat sheet. This is why the sections in my videos lie so flat. It is largely do to the correct temperature. As we cut colder and colder we get more and more curling and shattering. If you are starting with a very cold block warming with the thumb will get you down to the same temp. But if it takes more than a few seconds to get your section, warming will need to be repeated as the temp will quickly fall back down to the core temp of the block. Thanks for visiting my web site. Gail thanks for the complement it means a lot coming from you. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From contact <@t> excaliburpathology.com Tue Oct 3 13:31:16 2006 From: contact <@t> excaliburpathology.com (P Pierce) Date: Tue Oct 3 13:31:26 2006 Subject: [Histonet] C4d Antibody Message-ID: <20061003183117.51259.qmail@web50105.mail.yahoo.com> American Research Products has an excellant C4d antibody. This is a link to their page. http://www.arp1.com/search.php?keyword=c4d&category=&Submit=Search&what=search Here is a link to pics of slides I have stained using their C4d: http://www.immunoportal.com/modules.php?set_albumName=album01&op=modload&name=Gallery&file=index&include=view_album.php&page=3 Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From bhewlett <@t> cogeco.ca Tue Oct 3 13:32:32 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Oct 3 13:32:40 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? References: <20061003175509.97990.qmail@web61218.mail.yahoo.com> Message-ID: <008401c6e71a$4a9b3750$6500a8c0@mainbox> Rene, Sorry to pursue the 'can of worms' further but; The coefficient of infusibility for formaldehyde given in Bancroft is NOT based on Medewar's work, it is obtained from Baker (1958) Baker based this on the work of Tellyesniczky(1926) who used thick tissue samples and long fixation times. T. observed the visible changes and concluded a fixation time(wrongly, since he did not know that fixatives follow the laws of diffusion) in mm/hour. Baker calculated a coefficient of 0.78 from this data. Medewar(1941) proved that fixatives follow the laws of diffusion. He used plasma clots and stated a coefficient of 5.5 and commented that it was probably less, due to the nature of cell walls. Baker repeated the experiments using gelatin/albumen gels to more closely mimic tissue. He quotes a coefficient of 3.6 and again points out that penetration in tissue would probably be less. It is then that he mentions the data of Tellyesniczky and suggests 0.78. The work of both Fox(1985) and of Helander(1994) using C14 labelled formaldehyde indicates that the K really MUST be at least 2.0 and most probably 3.5. The numbness that you feel in your fingers after a few seconds is due to penetration of Methylene glycol not to formaldehyde binding! Bryan ----- Original Message ----- From: Rene J Buesa To: Bryan Hewlett ; GT Hebert ; histonet@lists.utsouthwestern.edu Sent: Tuesday, October 03, 2006 1:55 PM Subject: Re: [Histonet] Proof that fixation in formaldehyde solution can bereversible? Bryan: This is I think the "can of worms" that was mentioned before. If you put your fingers in formalin for just a few seconds you sill feel the numbness cause by the quick fixation and this same quick fixation is the one preventing the penetration with less and less penetration as more and more tissue is fixed. The coefficients of diffusability for major fixing agents (Bancroft & Stevens: Theory and practice of histological techniques", Table 2.1 based on reserach by Medawar) are as follows: potassium dichromate ---1.33 acetic acid ----1.2 ethanol---1.0 mercuric chloride--0.78 to 0.84 formaldehyde---0.78 That is why the combination of some of these reagents are able to take advantage of the quick fixation by formalin with the high penetration rates of the others. That is why to obtain a proper fixation with NBF it is required, not only at least 24 hours, but also why almost any processing protocol includes 1 or 2 initial station for a secondary fixation. Ren? J. Bryan Hewlett wrote: Rene, You wrote; >>Formalin is an extremely fast fixative but with a very slow rate of >>penetration, ....<< Actually, the reverse is the case!< BR>Formaldehyde has very fast penetration(possibly the fastest that we use) with a diffusion coefficient(K) of at least 2.0 and most likely 3.5. Compared to alcohol with a diffusion coefficient(K) of 0.8-1.0. Penetration can be calculated from the following; d = K times the square root of time, where d = distance in mm and time is in hours. However, the real problem is fixation. This is very slow, taking at least 24 hours at RT for 90% of formaldehyde binding to occur. This is due to the 'clock' reaction exhibited by formaldehyde solutions. Once the binding has occurred , then more cross-linking can proceed. See; Fox CH et al. Formaldehyde fixation. J. Histochem. Cytochem. 1985; 33, 845-853. Bryan ----- Original Message ----- From: "Rene J Buesa" To: "GT Hebert" ; Sent: Tuesday, October 03, 2006 1:04 PM Subject: Re: [Histonet] Proof that fixation in formaldehyde solution can bereversible? > GTH: > A piece of tissue sitting in PBS for 1 month after being fixed for 24-48 > hours only is likely to have suffered tissue decomposition at the center > of it, specially if it was a thick piece. Formalin is an extremely fast > fixative but with a very slow rate of penetration, this would have > determined external fixation but not complete fixation. > Fixation is not really reversible in a strict way and that is why you > need a strong Heat Inducet Epitope Retrieval (HIER) before IHC to "undo" > or "unfix" the crosslinkage produced by formalin. > The damage to the tissue could have been caused by improper storage = PBS > at room temperature during 1 month. If processed that tissue is very > likely to present altered microscopic appearance. > Just my opinion! > Ren? J. > &g t; GT Hebert wrote: > Hello, > > I am in a heated debate with an investigator regarding his samples. They > were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure > exactly which one) and then switch to PBS for shipment. They sat at room > temperature for over 1 month before being processed routinely and embedded > in paraffin wax. > > Can someone tell me if indeed the fixation is reversible, or once they > have been fixed for over 24hrs they remain fixed?? Can anyone refer me to > books or papers that talk about this?? > > Also, what affect on antigenicity will such storage in PBS have on these > samples? > > Thank you all so much for your help. > > G. H. > Cambridge, MA > > > > Key words: > NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, > reverse, reversible. > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates > starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------------------ Want to be your own boss? Learn how on Yahoo! Small Business. From contact <@t> excaliburpathology.com Tue Oct 3 14:35:33 2006 From: contact <@t> excaliburpathology.com (P Pierce) Date: Tue Oct 3 14:35:46 2006 Subject: [Histonet] C4d Antibody Message-ID: <20061003193534.90889.qmail@web50113.mail.yahoo.com> Forgot to mention the C4d was on formalin fixed, paraffin embedded tissue with citrate buffer HIER and using Dako's Envision for detection. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From gcallis <@t> montana.edu Tue Oct 3 15:11:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Oct 3 15:11:19 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: <20061003180144.47575.qmail@web30406.mail.mud.yahoo.com> References: <20061003180144.47575.qmail@web30406.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20061003135917.01b73480@gemini.msu.montana.edu> Hi Stephen, You are very welcome! It was a pleasure to visit with you at Phoenix NSH S/C. Keep up the good work creating those clever tools you have for cryosectioning!!! Your waffle weave "grid" (square and rectangular) metal disks are superbly designed for holding frozen tissue blocks - the BEST!!! especially for undecalcified bone cryosectioning and they fit in my 1850's, all three of them! Take care Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 12:01 PM 10/3/2006, you wrote: >Hi Ion, > > In our Leica !850's I use a blade angle of 5 with our low profile > blades. I know I > would not have much luck at a negative angle. > As far as curling tissue, in my experience the best temperature for > cutting > most tissus (except fat) is the warmest temp that the tissue will cut > without crumpling. Try starting to cut a touch on the warm side. Press > a piece > of cryostat temp metal ( I use my over-chuck blocks for this) on the > block face a > few seconds at a time until the tissue starts to cut. When the tissue > is just > cold enough to cut it will float off in a flat sheet. This is why the > sections in my > videos lie so flat. It is largely do to the correct temperature. As we > cut colder and colder we get more and more curling and shattering. If you > are starting with a very > cold block warming with the thumb will get you down to the same temp. > But if it > takes more than a few seconds to get your section, warming will need > to be > repeated as the temp will quickly fall back down to the core temp of > the block. > Thanks for visiting my web site. Gail thanks for the complement it > means a lot coming from you. > > > > > > >Stephen Peters M.D. >Vice Chairman of Pathology >Hackensack University Medical Center >201 996 4836 > >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellinr <@t> amgen.com Tue Oct 3 15:18:43 2006 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Tue Oct 3 15:19:11 2006 Subject: [Histonet] Using lectin BS-1 for staining endothelial cells Message-ID: <16834C6DFFA6004C88DE4507FB8AE544053E7C10@wa-mb4-sea.amgen.com> Gabriel, It is always hard to come up with optimal solution to any staining problem via internet since there are so many variables in procedure. One you might consider, if you haven't already, is the buffer used in your staining diluent. For biotinylated lectins, we always use HEPES buffer (not PBS). Many lectins require Mg++ or Ca++ to bind optimally and such ions will precipitate out in PBS. In fact if you look at the Vector website, under information for biotinylated Griffonia (their genus name for Bandieraea) simplicifola I, it states that "binding appears to require divalent cations such as calcium and magnesium". Ray Raymond Koelling Research Scientist, Pathology Amgen Corp. Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gabriel Anesetti Sent: Monday, October 02, 2006 3:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Using lectin BS-1 for staining endothelial cells Hello Histonetteers, I am having problems with the staining of rat endothelial cells in paraffin sections using a biotin labeled lectin from Bandeiraea Simplicifolia BS-1 (from Sigma). The lectin does not appear to be staining any of the endothelial cells. I have tried using citrate buffer for antigen retrieval and DAB, DAB + NiCl or FICT to visualize the site of reaction. Sections are from ovary grafts and have very new blood vessels forming. Any information would be helpful. Thanks Gabriel Anesetti Histology and Embryology Departament School of Medicine Montevideo, Uruguay ganesett@fmed.edu.uy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Oct 3 16:27:58 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 3 16:30:58 2006 Subject: AW: [Histonet] Proof that fixation in formaldehyde solution can bereversible? In-Reply-To: <000301c6e711$55d80ac0$eeeea8c0@dielangs.at> References: <000301c6e711$55d80ac0$eeeea8c0@dielangs.at> Message-ID: <4522D5DE.1080303@umdnj.edu> Gudrun is correct, formaldehyde penetrates quickly but fixes slowly. Geoff Gudrun Lang wrote: > Sorry Rene, >but isn't it the other way round? That penetration is rather fast and >fixation in the sense of making methylenbridges is rather slowly? So tissue >of 5 mm is penetrated within a few hours, but fixation takes much longer? > >I also learned that fixation with formaldehyd can be reversed by washing in >tapwater for several weeks. In some old books they recommend washing the >tissue the same duration as fixation was done. > >Gudrun > >-----Urspr?ngliche Nachricht----- >Von: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J >Buesa >Gesendet: Dienstag, 03. Oktober 2006 19:05 >An: GT Hebert; histonet@lists.utsouthwestern.edu >Betreff: Re: [Histonet] Proof that fixation in formaldehyde solution can >bereversible? > >GTH: > A piece of tissue sitting in PBS for 1 month after being fixed for 24-48 >hours only is likely to have suffered tissue decomposition at the center of >it, specially if it was a thick piece. Formalin is an extremely fast >fixative but with a very slow rate of penetration, this would have >determined external fixation but not complete fixation. > Fixation is not really reversible in a strict way and that is why you need >a strong Heat Inducet Epitope Retrieval (HIER) before IHC to "undo" or >"unfix" the crosslinkage produced by formalin. > The damage to the tissue could have been caused by improper storage = PBS >at room temperature during 1 month. If processed that tissue is very likely >to present altered microscopic appearance. > Just my opinion! > Ren? J. > >GT Hebert wrote: > Hello, > >I am in a heated debate with an investigator regarding his samples. They >were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure >exactly which one) and then switch to PBS for shipment. They sat at room >temperature for over 1 month before being processed routinely and embedded >in paraffin wax. > >Can someone tell me if indeed the fixation is reversible, or once they have >been fixed for over 24hrs they remain fixed?? Can anyone refer me to books >or papers that talk about this?? > >Also, what affect on antigenicity will such storage in PBS have on these >samples? > >Thank you all so much for your help. > >G. H. >Cambridge, MA > > > >Key words: >NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, >reverse, reversible. > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates >starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Want to be your own boss? Learn how on Yahoo! Small Business. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From liz <@t> premierlab.com Tue Oct 3 16:42:32 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 3 16:38:21 2006 Subject: [Histonet] Amino Cupric Silver method In-Reply-To: <20061003133500.k0khdepy7ocs0ksk@webmail4.isis.unc.edu> Message-ID: <001301c6e734$d4e87e80$0300a8c0@domain.Premier> Robert I have a pdf of one, I'll send in a separate e-mail Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rschoon@email.unc.edu Sent: Tuesday, October 03, 2006 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amino Cupric Silver method Can anyone out there give me a refferance to a Amino Cupric Silver method (ACS). TIA Robert Schoonhoven _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1787 (20061002) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From gcallis <@t> montana.edu Tue Oct 3 16:45:35 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Oct 3 16:45:56 2006 Subject: Buffers for RE: [Histonet] Using lectin BS-1 for staining endothelial cells In-Reply-To: <16834C6DFFA6004C88DE4507FB8AE544053E7C10@wa-mb4-sea.amgen. com> References: <16834C6DFFA6004C88DE4507FB8AE544053E7C10@wa-mb4-sea.amgen.com> Message-ID: <6.0.0.22.1.20061003152616.01bd9e18@gemini.msu.montana.edu> Ray is correct on the problem of using PBS with lectins. After much discussion with Vector's Craig Pow (Technical service representative), he indicated not ALL lectins are affected by PBS. We have had successful staining with UEA1 and PNA using Dulbeccos PBS. More recently, we use TRIS buffer exclusively for all lectin staining. There is also "Lectin Buffer" that is TRIS based on the chance you do not have HEPES buffer. See recipe below: TRIS base 60.57 g sodium chloride 87 g Magnesium chloride 2.03g calcium chloride 1.11g Dissolve in 800 mls distilled water. Adjust pH to 7.6 with concentrated HCl, then bring to final volume of 1 liter. This is a 10X solution, can be stored in the refrigerator, and diluted 1:10 when needed. Or you can bring this 0ne liter of 10X to a final volume of 10 liter using distilled water. What you have is a popular immunostaining buffer, 0.05M TBS buffer but with the Mg and Ca added. The lectin buffer comes from an inexpensive, excellent book titled Lectin Histochemistry, a concise practical handbook Brooks SA, Leathem AJC, Schumacher U. ISBN# 1859961002 . Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 t 02:18 PM 10/3/2006, you wrote: >Gabriel, >It is always hard to come up with optimal solution to any staining problem >via internet since there are so many variables in procedure. One you might >consider, if you haven't already, is the buffer used in your staining >diluent. For biotinylated lectins, we always use HEPES buffer (not PBS). >Many lectins require Mg++ or Ca++ to bind optimally and such ions will >precipitate out in PBS. In fact if you look at the Vector website, under >information for biotinylated Griffonia (their genus name for Bandieraea) >simplicifola I, it states that "binding appears to require divalent cations >such as calcium and magnesium". > >Ray >Raymond Koelling >Research Scientist, Pathology >Amgen Corp. >Seattle, WA > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gabriel >Anesetti >Sent: Monday, October 02, 2006 3:51 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Using lectin BS-1 for staining endothelial cells > >Hello Histonetteers, >I am having problems with the staining of rat endothelial cells in >paraffin sections using a biotin labeled lectin from Bandeiraea >Simplicifolia BS-1 (from Sigma). The lectin does not appear to be >staining any of the endothelial cells. I have tried using citrate buffer >for antigen retrieval and DAB, DAB + NiCl or FICT to visualize the site >of reaction. Sections are from ovary grafts and have very new blood >vessels forming. Any information would be helpful. > From liz <@t> premierlab.com Tue Oct 3 16:57:28 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 3 16:53:08 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: <6.0.0.22.1.20061003135917.01b73480@gemini.msu.montana.edu> Message-ID: <001f01c6e736$eaf28390$0300a8c0@domain.Premier> I have to agree with Gayle I have Pathology Innovations chucks, base molds, etc. They work great, much better than the ones you get when you buy your cryostat. I frequently get frozen tissue that is not in OCT and the base molds from PI work great, I have embedded up to 5 different mouse tissues in the larger one and do not have any problems with sectioning. They also work great for fixed frozen sections. It's a great tool. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, October 03, 2006 2:11 PM To: Stephen Peters M.D.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Superfrost slides-Histonet question Hi Stephen, You are very welcome! It was a pleasure to visit with you at Phoenix NSH S/C. Keep up the good work creating those clever tools you have for cryosectioning!!! Your waffle weave "grid" (square and rectangular) metal disks are superbly designed for holding frozen tissue blocks - the BEST!!! especially for undecalcified bone cryosectioning and they fit in my 1850's, all three of them! Take care Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 12:01 PM 10/3/2006, you wrote: >Hi Ion, > > In our Leica !850's I use a blade angle of 5 with our low profile > blades. I know I > would not have much luck at a negative angle. > As far as curling tissue, in my experience the best temperature for > cutting > most tissus (except fat) is the warmest temp that the tissue will cut > without crumpling. Try starting to cut a touch on the warm side. Press > a piece > of cryostat temp metal ( I use my over-chuck blocks for this) on the > block face a > few seconds at a time until the tissue starts to cut. When the tissue > is just > cold enough to cut it will float off in a flat sheet. This is why the > sections in my > videos lie so flat. It is largely do to the correct temperature. As we > cut colder and colder we get more and more curling and shattering. If you > are starting with a very > cold block warming with the thumb will get you down to the same temp. > But if it > takes more than a few seconds to get your section, warming will need > to be > repeated as the temp will quickly fall back down to the core temp of > the block. > Thanks for visiting my web site. Gail thanks for the complement it > means a lot coming from you. > > > > > > >Stephen Peters M.D. >Vice Chairman of Pathology >Hackensack University Medical Center >201 996 4836 > >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1788 (20061003) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From emathelier <@t> tmail.com Tue Oct 3 18:29:13 2006 From: emathelier <@t> tmail.com (Emilie) Date: Tue Oct 3 18:29:20 2006 Subject: [Histonet] MOHS Tech position desired in SE Florida Message-ID: <1159918154.2188F14A@dg11.dngr.org> Excellent tech with over 3 years experience looking for part time work, 1 to 4 days a week. I can work afternoons after 1pm mon-thurs and all day fridays. I produce quality sections at a fast turn around time. All interested parties may contact me at emathelier@tmail.com or 954-864-9390. --Emilie From Gervaip <@t> aol.com Tue Oct 3 20:19:31 2006 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Tue Oct 3 20:19:41 2006 Subject: [Histonet] Dan, the "chicken man"??? Message-ID: <597.5bab3ff.32546623@aol.com> Hi, I am trying to get in touch with Dan S. who was at the NSH and attended the "Writing a job description workshop" . He was out of Florida and recently moved to Ohio. Pearl Gervais From donna <@t> milestonemed.com Tue Oct 3 20:42:06 2006 From: donna <@t> milestonemed.com (Donna Willis) Date: Tue Oct 3 20:42:29 2006 Subject: [Histonet] Looking for Ronnie Houston Message-ID: <000c01c6e756$4f4259f0$6d63d9a6@LAPTOP02> Does anyone in Histoland know where I might contact Ronnie Houston. I understand that he recently changed positions. Thanks in advance, Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager Gulf Coast Sales 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell From omnivore98 <@t> yahoo.com Tue Oct 3 20:53:42 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Tue Oct 3 20:53:47 2006 Subject: [Histonet] pure ethanol switch to reagent grade 100% Message-ID: <20061004015342.1100.qmail@web31307.mail.mud.yahoo.com> Histonetters, I hope to get input from our vast group here on switching from pure ethanol/200 proof to 100% reagent grade alcohol. The reagent grade is by RA, 90% ethyl, 5.5% methyl, and 4.5% isopropyl. My main concern was for the dehydration properties of the reagent grade to the pure but, I feel confident that there will not be any real problems with our processing or the staining. I would welcome any feedback to your switch over experiences. Lastly, we do not use this alcohol to make any reagents, just in its original state. Heather --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From lpwenk <@t> sbcglobal.net Wed Oct 4 04:52:17 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Oct 4 04:52:56 2006 Subject: [Histonet] Amino Cupric Silver method In-Reply-To: <20061003133500.k0khdepy7ocs0ksk@webmail4.isis.unc.edu> Message-ID: <000f01c6e79a$c7685080$faf62d4b@HPPav2> Try this - with pictures even! http://www.neuroscienceassociates.com/Stains/silver_degen.htm Peggy A. Wenk, HTL(ACP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rschoon@email.unc.edu Sent: Tuesday, October 03, 2006 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amino Cupric Silver method Can anyone out there give me a refferance to a Amino Cupric Silver method (ACS). TIA Robert Schoonhoven _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgibbon <@t> qltinc.com Wed Oct 4 09:30:47 2006 From: kgibbon <@t> qltinc.com (Kevin Gibbon) Date: Wed Oct 4 09:31:02 2006 Subject: [Histonet] pure ethanol switch to reagent grade 100% Message-ID: <66A24278845C594C98E5ECB840D7BFB5028C0A80@VAN-EXCH-VS1.qltinc.com> Hi Heather, I have always used reagent grade alcohols for dehydration and staining. The one I use at the moment is 85% ethanol and 15% other mixed alcohols. Kevin Kevin Gibbon Manager, Histology QLT Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Tuesday, October 03, 2006 6:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pure ethanol switch to reagent grade 100% Histonetters, I hope to get input from our vast group here on switching from pure ethanol/200 proof to 100% reagent grade alcohol. The reagent grade is by RA, 90% ethyl, 5.5% methyl, and 4.5% isopropyl. My main concern was for the dehydration properties of the reagent grade to the pure but, I feel confident that there will not be any real problems with our processing or the staining. I would welcome any feedback to your switch over experiences. Lastly, we do not use this alcohol to make any reagents, just in its original state. Heather --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. From mward <@t> wfubmc.edu Wed Oct 4 09:30:32 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Oct 4 09:35:53 2006 Subject: [Histonet] H. pylori Message-ID: <61135F0455D33347B5AAE209B903A30416990A6D@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this request for a colleague. They are interested in doing H. pylori immuno stains but will not be using an automated stainer. Does anyone have a suggestion about which detection kits and antibody they could use for manual staining? Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From laurie.colbert <@t> huntingtonhospital.com Wed Oct 4 10:24:04 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Oct 4 10:24:18 2006 Subject: [Histonet] Sleeve Covers Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653DB0@EXCHANGE1.huntingtonhospital.com> Does anyone know where I can purchase sleeve covers? Laurie Colbert From beldorth.msu+hist <@t> gmail.com Wed Oct 4 10:42:01 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Wed Oct 4 10:42:08 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question In-Reply-To: <6.0.0.22.1.20061003163439.01bee570@gemini.msu.montana.edu> References: <20061003180144.47575.qmail@web30406.mail.mud.yahoo.com> <6.0.0.22.1.20061003135917.01b73480@gemini.msu.montana.edu> <6.0.0.22.1.20061003163439.01bee570@gemini.msu.montana.edu> Message-ID: Gayle, Stephen and Mari Ann, Thanks again for the advice on sectioning. This has diverged quite a bit from my two original concerns (why my sections are falling off after washing, and trying to find a decent water-stable counterstain to NBT/BCIP), but this revives some issues and questions I had a few months ago that I never satisfactorily resolved. Gayle, I tried your advice on stopping just before the section detaches and it works great (now if only I could consistently catch that dang curl). I also like your idea of holding onto the wheel rather than the handle. MUCH better control that way. I was just thinking that there is a generation of kids behind me that would be really good at sectioning tissue -- it requires a great deal of coordination between both hands and what you are seeing, and there are none better then these kids who have mastered fast-paced first-person-shooter video games using control pads with dual joysticks and eight or more buttons. They are also great at sitting in one spot for hours at a time doing the same thing over and over and over. A concerted effort should be made to get them into labs . . . I will head to a art supply store sometime and check out brushes, too. I bought a stiff brush months ago to replace the soft brushes we had been using, but for a different reason: the soft brushes tend to build up static and getting the sections off of them is a nightmare, which led to many not-so-private curses. I did trim it at an angle after finding Stephen's website, but I don't think it is quite as stiff. Lastly, can you send along both powerpoints? Stephen, since you are in audience, can I ask about your technique of dipping in 95% Ethanol after mounting a frozen section? My main question is whether or not this can be done to a slide that will have multiple sections on it ( i.e., can I mount, dip, mount, dip, mount, etc.) or whether it is a one time only deal. Ion From tp2 <@t> medicine.wisc.edu Wed Oct 4 10:59:09 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Wed Oct 4 10:59:50 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question Message-ID: <452393FD020000DF00001D10@gwmail.medicine.wisc.edu> You bring up a good point. When I interviewed for my first histology position I was asked if I had any hobbies that required manual dexterity in both hands at once. I mentioned that I played guitar and also that I played video games. Some people on the pannel must have thought I was nuts because they said, "Video game?" It was then that I pointed out how many buttons (up to 12), sticks (1 or 2), and a directional pad were on a video game controller these days. At that point they realized that I wasn't joking around. Tom Pier >>> "I.B." 10/04/06 10:42 AM >>> Gayle, Stephen and Mari Ann, Thanks again for the advice on sectioning. This has diverged quite a bit from my two original concerns (why my sections are falling off after washing, and trying to find a decent water-stable counterstain to NBT/BCIP), but this revives some issues and questions I had a few months ago that I never satisfactorily resolved. Gayle, I tried your advice on stopping just before the section detaches and it works great (now if only I could consistently catch that dang curl). I also like your idea of holding onto the wheel rather than the handle. MUCH better control that way. I was just thinking that there is a generation of kids behind me that would be really good at sectioning tissue -- it requires a great deal of coordination between both hands and what you are seeing, and there are none better then these kids who have mastered fast-paced first-person-shooter video games using control pads with dual joysticks and eight or more buttons. They are also great at sitting in one spot for hours at a time doing the same thing over and over and over. A concerted effort should be made to get them into labs . . . I will head to a art supply store sometime and check out brushes, too. I bought a stiff brush months ago to replace the soft brushes we had been using, but for a different reason: the soft brushes tend to build up static and getting the sections off of them is a nightmare, which led to many not-so-private curses. I did trim it at an angle after finding Stephen's website, but I don't think it is quite as stiff. Lastly, can you send along both powerpoints? Stephen, since you are in audience, can I ask about your technique of dipping in 95% Ethanol after mounting a frozen section? My main question is whether or not this can be done to a slide that will have multiple sections on it ( i.e., can I mount, dip, mount, dip, mount, etc.) or whether it is a one time only deal. Ion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From soofias2 <@t> yahoo.com Wed Oct 4 11:05:44 2006 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Wed Oct 4 11:05:52 2006 Subject: [Histonet] Sleeve Covers In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628005653DB0@EXCHANGE1.huntingtonhospital.com> Message-ID: <20061004160544.72519.qmail@web39507.mail.mud.yahoo.com> Cardinal Health sterile disposible sleeves you can by it from Fisher. Soofia Laurie Colbert wrote: Does anyone know where I can purchase sleeve covers? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From gcallis <@t> montana.edu Wed Oct 4 11:22:44 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Oct 4 11:22:54 2006 Subject: [Histonet] Manual dexterity questions for potential techs In-Reply-To: <452393FD020000DF00001D10@gwmail.medicine.wisc.edu> References: <452393FD020000DF00001D10@gwmail.medicine.wisc.edu> Message-ID: <6.0.0.22.1.20061004101027.01b57e80@gemini.msu.montana.edu> Tom and all, This topic made me chuckle. In pre-video pad/game days, I asked students and potential technicians if they could knit. The majority were girls, the question caused raised eyebrows and laughs from the guys although many men do knit very well. It still causes chuckles and now the guys usually answer that they tie flies for fly fishing. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From petepath <@t> yahoo.com Wed Oct 4 11:24:49 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Oct 4 11:25:01 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question Message-ID: <20061004162449.18801.qmail@web30415.mail.mud.yahoo.com> Ion, In general once you have dipped the slide in ETOH you cannot put another section on while it is wet with ETOH . I do not see why you couldn't try to put another on after the ETOH has dried. You can also put multiple pieces on the slide and fix them all at once but the first section will show some drying artifact. I guess you could also put a first section toward the label, then dip only the lable half of the slide. Then put a second section toward the opposite end of the slide and dip that half. As far as FS brushes are concerned. I actually went to an art supply store and tried a variety of them. For me I settle on the stiffest which gave me the most control over the curling section. I use Chinese bristle brushes. Number 1 or 2 flat or bright type. ( 3/16 and 1/4 inch). I cut them at an angle with a sharp blade to match the angle I am holding it. I also cut the length of the handle down to a practical size ( they are long artist brushes). These are about $3 at your art supply store. I buy them in bulk from the solo brush company prepare and sell them for the same $3. Here is the link if anyone wants to buy them in quantity. http://www.solobrushes.com/WebCatg2.asp?Cat2ID=12&Cat1ID=6 Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From petepath <@t> yahoo.com Wed Oct 4 11:29:30 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Oct 4 11:29:40 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question Message-ID: <20061004162930.2157.qmail@web30406.mail.mud.yahoo.com> Ion, One last thing. You mentioned tissue sticking to the brushes. I start my day by cleaning the FS brushes with a touch of soap and water followed by drying, quick ETOH dip and dry, quick Xylene dip and dry. This takes less than a minute and will decrease the stickyness. Steve Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From petepath <@t> yahoo.com Wed Oct 4 11:44:10 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Oct 4 11:44:17 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question Message-ID: <20061004164410.8174.qmail@web30406.mail.mud.yahoo.com> Tom, You bring up manual dexterity and the importance of this in the frozen section process. I have played classsical guitar for over 40 years. The brush technique I describe on my web site is in many ways modeled after concepts I have taken from guitar technique, i.e. conservation of motion, maximizing our control through stability if the hand and proper ergonomic positioning of our hands and body to maximize our fine motor ability. Even passing the technique to students by holding their limp hands and moving the brush and wheel together so that they develop muscle memory for this technically challenging task came out of my music training. The goal of continous motion cutting is a skill which can be easily developed by anyone. Like a musical instrument, it comes with dicipline, practice and attention to technique. Steve Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From JWEEMS <@t> sjha.org Wed Oct 4 11:48:06 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 4 11:48:34 2006 Subject: [Histonet] Re: Superfrost slides-Histonet question Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEECBF@sjhaexc02.sjha.org> And I got laughed at when I asked applicants if they could play the guitar! Where were you then!!!? j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stephen Peters M.D. Sent: Wednesday, October 04, 2006 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Superfrost slides-Histonet question Tom, You bring up manual dexterity and the importance of this in the frozen section process. I have played classsical guitar for over 40 years. The brush technique I describe on my web site is in many ways modeled after concepts I have taken from guitar technique, i.e. conservation of motion, maximizing our control through stability if the hand and proper ergonomic positioning of our hands and body to maximize our fine motor ability. Even passing the technique to students by holding their limp hands and moving the brush and wheel together so that they develop muscle memory for this technically challenging task came out of my music training. The goal of continous motion cutting is a skill which can be easily developed by anyone. Like a musical instrument, it comes with dicipline, practice and attention to technique. Steve Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From petepath <@t> yahoo.com Wed Oct 4 11:53:13 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Oct 4 11:53:21 2006 Subject: [Histonet] Superfrost slides-Histonet question Message-ID: <20061004165313.6818.qmail@web30408.mail.mud.yahoo.com> Ion, In general once you have dipped the slide in ETOH you cannot put another section on while it is wet with ETOH . I do not see why you couldn't try to put another on after the ETOH has dried. You can also put multiple pieces on the slide and fix them all at once but the first section will show some drying artifact. I guess you could also put a first section toward the label, then dip only the lable half of the slide. Then put a second section toward the opposite end of the slide and dip that half. As far as FS brushes are concerned. I actually went to an art supply store and tried a variety of them. For me I settle on the stiffest which gave me the most control over the curling section. I use Chinese bristle brushes. Number 1 or 2 flat or bright type. ( 3/16 and 1/4 inch). I cut them at an angle with a sharp blade to match the angle I am holding it. I also cut the length of the handle down to a practical size ( they are long artist brushes). These are about $3 at your art supply store. I buy them in bulk from the solo brush company prepare and offer them for the same $3. Here is the link if anyone wants to buy them in quantity. http://www.solobrushes.com/WebCatg2.asp?Cat2ID=12&Cat1ID=6 From beldorth.msu+hist <@t> gmail.com Wed Oct 4 12:05:12 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Wed Oct 4 12:05:17 2006 Subject: [Histonet] Superfrost slides-Histonet question In-Reply-To: <20061004165313.6818.qmail@web30408.mail.mud.yahoo.com> References: <20061004165313.6818.qmail@web30408.mail.mud.yahoo.com> Message-ID: Stephen, OK, that is what I figured. I have been hesitant to try it because I need to mount multiple sections on each slide, and I wasn't sure if the EtOH would in some way alter the adhesive properties of the slide (I have been having enough problems with that, don't want to add to it). Ion On 10/4/06, Stephen Peters M.D. wrote: > > Ion, > > In general once you have dipped the slide in ETOH you cannot put another > section > on while it is wet with ETOH . I do not see why you couldn't try to put > another on > after the ETOH has dried. You can also put multiple pieces on the slide > and fix > them all at once but the first section will show some drying artifact. > I guess you could also put a first section toward the label, then dip > only the lable > half of the slide. Then put a second section toward the opposite end of > the slide > and dip that half. > As far as FS brushes are concerned. I actually went to an art supply > store and tried > a variety of them. For me I settle on the stiffest which gave me the > most control > over the curling section. > I use Chinese bristle brushes. Number 1 or 2 flat or bright type. ( 3/16 > and 1/4 inch). > I cut them at an angle with a sharp blade to match the angle I am > holding it. I also > cut the length of the handle down to a practical size ( they are long > artist brushes). > These are about $3 at your art supply store. I buy them in bulk from the > solo > brush company prepare and offer them for the same $3. Here is the link > if > anyone wants to buy them in quantity. > http://www.solobrushes.com/WebCatg2.asp?Cat2ID=12&Cat1ID=6 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Eric <@t> ategra.com Tue Oct 3 21:37:30 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Wed Oct 4 12:11:42 2006 Subject: [Histonet] Update Oct 03 2006 - Temporary and Permanent Histology opportunites near you Message-ID: Hi - Fellow-Histonetters How are things there in MN/TX/CA/MA/USA ? Below is the updated list of both temp and perm Histology jobs, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech jobs are permanent full-time unless indicated otherwise Also, if your not interested in any of these great opportunities and prefer a different location, please call me and let me know where and I can find it for you. Permanent Histology Jobs: ------------------------------------------------------ 1. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 2. Washington, D.C. - Histotech -perm and temp 3. Northern New Jersey - Histotech - perm 4. Boston Mass - Histotech - one Senior Histotech, One not so Senior Histotech 5. Boston Mass - Histotech - Histotech -perm 6. Massachusetts (North of Boston) - perm - Bench Histotech 7. Central Florida -perm- Histotech 8. Southeast Florida - Treasure Coast - perm - Histotech 9. Southeast Florida - perm - Histotech 10. Southwest Florida - perm - Bench Histotech/ Supervisor 11. Florida, West Coast - both temp & perm openings- Bench Histotech 12. New Hampshire perm openings - Bench Histotech 13. New York ( Syracuse area) - Bench Histotech- perm 14. Central Florida - Bench Histotech- perm 15. New York (Long Island)- Bench Histotech- perm 16. Virginia (Northern) - Bench Histotech - perm- NEW -----------------------------------end perm jobs ---------------------------------------------------- Temporary Assignments ------------------------------- 1. Alaska -Histo Tech Temp - minimum of 16 weeks 2. Washington,DC - Histotech - minimum of 6 weeks- also Temp to Perm 3. Massachusetts (Boston area) - Histo Tech minimum of 13 weeks (2 people) 4. Pennsylvania - Histotech- 6 months 5. New Mexico - Histotech- 10-12 weeks 6. Florida (South East)(Treasure Coast)- minimum of 4 weeks possibly longer -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From pruegg <@t> ihctech.net Wed Oct 4 12:33:34 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Oct 4 12:33:45 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution canbereversible? In-Reply-To: Message-ID: <00ab01c6e7db$38e6ed30$6601a8c0@Patsy> " Fixation is generally carried on by the alcohols used for the processing.? Here in lies the problem for many antigens "unintended consequences" you might say. If the sample is not adequately fixed-proteins protected by crosslinking, the alcohols can destroy the protein of interest. We need to fix for a minimum of 24 hours to allow for crosslinking which will protect the proteins (especially surface proteins) from processing so that they are still there and can be accessed after unmasking the formalin fixation. If certain proteins are not protected from tissue processing they will be lost forever and no amount of epitope retrieval will restore them. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, October 03, 2006 10:49 AM To: GT Hebert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proof that fixation in formaldehyde solution canbereversible? This is really opening a barrel of worms but... Re formaldehyde solutions. If tissues are fixed for a few hours in standard formalin solutions then it is possible to reverse the fixation process by washing. It is a reversible process in that the bonds that have been formed are generally temporary and can be broken. Technically if you wish for "adequate or true fixation" then Pearse provides the theory for why this should be for about 1 week. (The fixation process does in fact continue for years). Of course no one fixes for one week except by accident. However the fixation for 24 to 48 hours is only an initial fixation. Fixation is generally carried on by the alcohols used for the processing. A. G. E. Pearse "Histochemistry Theoretical and Applied: volume 1 Preparative and Optical Technology." Churchill Livingstone. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Tuesday, October 03, 2006 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Proof that fixation in formaldehyde solution can bereversible? Hello, I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax. Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this?? Also, what affect on antigenicity will such storage in PBS have on these samples? Thank you all so much for your help. G. H. Cambridge, MA Key words: NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Wed Oct 4 14:05:04 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Oct 4 14:05:11 2006 Subject: [Histonet] H. pylori In-Reply-To: <61135F0455D33347B5AAE209B903A30416990A6D@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30416990A6D@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <8C8B5FF22781C67-1498-595D@mblk-d23.sysops.aol.com> Biocare has a universal detection system that works great and we do it by hand.... Roxanne Soto HT(ASCP)QIHC Lab Manager Tampa, Florida -----Original Message----- From: mward@wfubmc.edu To: histonet@lists.utsouthwestern.edu Sent: Wed, 4 Oct 2006 10:30 AM Subject: [Histonet] H. pylori I am posting this request for a colleague. They are interested in doing H. pylori immuno stains but will not be using an automated stainer. Does anyone have a suggestion about which detection kits and antibody they could use for manual staining? Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From laurie.colbert <@t> huntingtonhospital.com Wed Oct 4 14:41:11 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Oct 4 14:41:19 2006 Subject: [Histonet] Sleeve Protectors Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653DB6@EXCHANGE1.huntingtonhospital.com> Thank you for all of the information on the sleeve protectors. I've already ordered some! Laurie From mward <@t> wfubmc.edu Wed Oct 4 14:50:44 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Oct 4 14:50:51 2006 Subject: [Histonet] H. pylori Message-ID: <61135F0455D33347B5AAE209B903A304169DD1BE@EXCHVS2.medctr.ad.wfubmc.edu> I would like to thank everyone who responded to my request for help. I have gotten many wonderful suggestions and I will pass them along to my colleague. Martha Ward From tanjie <@t> u.washington.edu Wed Oct 4 15:01:53 2006 From: tanjie <@t> u.washington.edu (Elaine Tanji) Date: Wed Oct 4 15:01:59 2006 Subject: [Histonet] Seattle Histo Tech Opening Message-ID: <45241331.902@u.washington.edu> Hello Everyone, Just wanted to update you that we still have an opening for a Histo Tech. Our HR department has been having some problems with their website and job posting so I have pasted the job description below. Please feel free to call or email me for details on this position. Thank you. Elaine -- Elaine Tanji Anatomic Pathology Laboratory Supervisor University of Washington Medical Center 206-598-6030 tanjie@u.washington.edu Join our award-winning team at University of Washington Medical Center (UWMC). Ranked among the nation?s top ten hospitals by US News and World Report (2005), UWMC prides itself on compassionate patient care as well as its pioneering medical advances. UWMC is operated by the University of Washington (UW) and serves as a training site for UW?s School of Medicine. The University of Washington is proud to be one of the nation?s premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement, artistic pursuits, and natural beauty. General Duties: The Anatomic Pathology department has an outstanding opportunity for a *Anatomic Pathology Technician.* The person in this role will be responsible for the following duties and tasks: Perform preparation of tissue for microscopic analysis * Receive and accession specimens, verifying that patient data is accurate * Can use computer to accession specimens * Knowledge of tissue fixation, dehydration, clearing, etc. * Prepare specimens by frozen section using a cryostat * Embed and orient tissue correctly in paraffin or OCT media * Cut thin sections of paraffin-embedded specimens using a rotary microtome * Coverslip microscopic slides with no air bubbles; label slides * Examine samples of stained slides with microscope to ascertain that results are satisfactory Perform histochemical procedures for identification of various tissue elements * Cut section of tissue to correct thickness to identify tissue element being stained * Perform staining procedures to identify fungus, fat, fibers, bacteria, by established methods * Prepare solutions and reagents in accordance with standard procedure * Ability to use pH meter, microwave, balance * Develop new staining procedures when necessary * Examine control slides of special stains to ascertain that results are satisfactory General knowledge of HISTOLOGY equipment * Perform routine maintenance of microtome and frozen section cryostat * Perform the daily maintenance of the automatic tissue processor and staining machines * Keep embedding centers cleaned and maintained * Properly load and unload cameras in the Gross Room * Record temperatures of instrumentation From Rcartun <@t> harthosp.org Wed Oct 4 17:34:49 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Oct 4 17:35:32 2006 Subject: [Histonet] c4d antibody In-Reply-To: <20061003115730.57611.qmail@web56502.mail.re3.yahoo.com> References: <20061003115730.57611.qmail@web56502.mail.re3.yahoo.com> Message-ID: <4523FEC902000077000023C6@hcnwgwds01.hh.chs> We use a rabbit anti-human C4d from American Research Products (Belmont, MA) for formalin-fixed, paraffin-embedded tissue. Their telephone number is (617) 489-1120. I have a beautiful digital image of C4d labeling in kidney tissue that I can send you if you're interested. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Ruser Rebecca 10/03/06 7:57 AM >>> Can anyone provide me with information on obtaining C4d antibody for use on paraffin sections? I found an article about an antibody from Biomedica but can't find where to purchase this antibody in the U.S. Thanks for any help you can give me. Rebecca Ruser --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Oct 4 23:22:50 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 4 23:23:09 2006 Subject: [Histonet] argentaffin vs argyrophil Message-ID: When performing an argyrophil stain (Grimelius) one must also per form an argentaffin stain (Fontana-Masson) to rule out the positive argenta you perfor substance for one Thank you, Jennifer MacDonald From AnthonyH <@t> chw.edu.au Thu Oct 5 01:05:09 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Oct 5 01:06:43 2006 Subject: [Histonet] argentaffin vs argyrophil Message-ID: Why not. This seems an ingenious way of detecting argyrophil cells only. This would be an interesting project. Stain various endocrine containing cells and other silver attracting substances (eg lipofuscin & melanin) with the two techniques and compare the results. Also include several argentaffinomas and argyrophilic tumours. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, 5 October 2006 2:23 PM To: 'Histonet' Subject: [Histonet] argentaffin vs argyrophil When performing an argyrophil stain (Grimelius) one must also per form an argentaffin stain (Fontana-Masson) to rule out the positive argenta=ffin staining in the Grimelius procedure. Would it work if you perfor=med a Grimelius on two slides, but left out the reducing substance for one =f the slides? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From BMolinari <@t> heart.thi.tmc.edu Thu Oct 5 05:32:29 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 5 05:32:36 2006 Subject: [Histonet] Sleeve Covers In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628005653DB0@EXCHANGE1.huntingtonhospital.com> Message-ID: Fisher Scientific Cat # 01-361-21 White sleeve protectors. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, October 04, 2006 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sleeve Covers Does anyone know where I can purchase sleeve covers? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mauger <@t> email.chop.edu Thu Oct 5 07:22:52 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Thu Oct 5 07:23:51 2006 Subject: [Histonet] c4d antibody Message-ID: Hi Richard, I am working this antibody up mainly for use on heart bxs,but I would like to see your picture of kidney if you would be so kind! Thanks,--by the way- you helped me out with cat scratch a while back. Just to let you know, it was working beautifully all along-my Pathologist was having trouble believing it until we got a very strongly + case!! Jo Mauger From hej01 <@t> health.state.ny.us Thu Oct 5 08:23:45 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Oct 5 08:23:59 2006 Subject: [Histonet] IHC on insects Message-ID: Hi Histonetters, I need to do IHC on FFPE insects. Does anyone have a good protocol? Helen Johnson ( hej01@health.state.ny.us ) (Tel: 518-474-8295) From c.weaver <@t> vla.defra.gsi.gov.uk Thu Oct 5 08:38:50 2006 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Thu Oct 5 08:51:56 2006 Subject: [Histonet] Modified ZN stain Message-ID: <1172D362B084D311986F0008C79F42C478CC72@vla22.cvlnt.vla.gov.uk> Hi - everyone - this is my first time on here. I wish to try staining Lawsonia intracellularis in gut sections, these are MZN positive in gut sgrapings but we can never get our MZN method to work satisfactorily. Anyone got any ideas or experience? Colin Weaver Histology Dept Veterinary Laboratory Agencies Regional Lab West House Station Road Thirsk North Yorks UK Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From pruegg <@t> ihctech.net Thu Oct 5 10:11:43 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 5 10:11:56 2006 Subject: [Histonet] H. pylori In-Reply-To: <8C8B5FF22781C67-1498-595D@mblk-d23.sysops.aol.com> Message-ID: <002201c6e890$92f37040$6601a8c0@Patsy> In my opinion, for an IHC protocol to be of value it must be flexible and I should be able to manipulate it manually, if it can only be used as is with a certain instrument, it is not for me. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Wednesday, October 04, 2006 1:05 PM To: mward@wfubmc.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] H. pylori Biocare has a universal detection system that works great and we do it by hand.... Roxanne Soto HT(ASCP)QIHC Lab Manager Tampa, Florida -----Original Message----- From: mward@wfubmc.edu To: histonet@lists.utsouthwestern.edu Sent: Wed, 4 Oct 2006 10:30 AM Subject: [Histonet] H. pylori I am posting this request for a colleague. They are interested in doing H. pylori immuno stains but will not be using an automated stainer. Does anyone have a suggestion about which detection kits and antibody they could use for manual staining? Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakerj <@t> umich.edu Thu Oct 5 11:00:46 2006 From: bakerj <@t> umich.edu (John Baker) Date: Thu Oct 5 11:02:51 2006 Subject: [Histonet] tissue coming off slides Message-ID: <4d17e246336fc0aaf90675b75d6110a5@umich.edu> Hi All, A group I work with just came to ask if there was a way of keeping frozen tissue sections from coming off a slide? It seems there technician used uncoated/regular slides to mount 100's of sections. The tissues were embedded in OCT and cut at 5 microns but of course are sliding off during any staining procedure. Is there a protocol of any sort to coat the slides after the fact to retain the sections through staining? I at least suggested they stain flat. Any suggestions are appreciated. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From godsgalnow <@t> aol.com Thu Oct 5 11:13:52 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Oct 5 11:14:10 2006 Subject: [Histonet] H. pylori In-Reply-To: <002201c6e890$92f37040$6601a8c0@Patsy> Message-ID: <8C8B6B06252E953-C88-17A8@mblk-d26.sysops.aol.com> This is an antibody that can be used with an instrument or by hand---as a matter of fact I do this particular antibody manually. But the question was about detection...and this detection kit also is purchased through Biocare and it a universal detection kit (poly or mono) and I get excellent results whether I use it on the machine or I use my little patties....... Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: pruegg@ihctech.net To: godsgalnow@aol.com; mward@wfubmc.edu; histonet@lists.utsouthwestern.edu Sent: Thu, 5 Oct 2006 11:11 AM Subject: RE: [Histonet] H. pylori In my opinion, for an IHC protocol to be of value it must be flexible and I should be able to manipulate it manually, if it can only be used as is with a certain instrument, it is not for me. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Wednesday, October 04, 2006 1:05 PM To: mward@wfubmc.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] H. pylori Biocare has a universal detection system that works great and we do it by hand.... Roxanne Soto HT(ASCP)QIHC Lab Manager Tampa, Florida -----Original Message----- From: mward@wfubmc.edu To: histonet@lists.utsouthwestern.edu Sent: Wed, 4 Oct 2006 10:30 AM Subject: [Histonet] H. pylori I am posting this request for a colleague. They are interested in doing H. pylori immuno stains but will not be using an automated stainer. Does anyone have a suggestion about which detection kits and antibody they could use for manual staining? Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Kari.Zajic <@t> HCAhealthcare.com Thu Oct 5 11:27:26 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Oct 5 11:27:44 2006 Subject: [Histonet] tissue coming off slides In-Reply-To: <4d17e246336fc0aaf90675b75d6110a5@umich.edu> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC716@ORLEV03.hca.corpad.net> Hi John! That used to happen to me and I changed all of my slides to + charged slides and it stopped. Now, I put the sectioned slides directly into Hematoxylin for staining, but I have had techs feel more comfortable putting them in 95% Alcohol first to fix. I personally haven't had negative or positive feedback on the stain quality either way (my Pathologists didn't notice a difference). I also heard just yesterday from a tech that she puts them into Formalin before staining...this I have never heard before, but she claims it works. Good luck, hope this helped a tad... Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of John Baker Sent: Thursday, October 05, 2006 12:01 PM To: Histonet Subject: [Histonet] tissue coming off slides Hi All, A group I work with just came to ask if there was a way of keeping frozen tissue sections from coming off a slide? It seems there technician used uncoated/regular slides to mount 100's of sections. The tissues were embedded in OCT and cut at 5 microns but of course are sliding off during any staining procedure. Is there a protocol of any sort to coat the slides after the fact to retain the sections through staining? I at least suggested they stain flat. Any suggestions are appreciated. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu Oct 5 11:32:49 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Oct 5 11:33:18 2006 Subject: [Histonet] tissue coming off slides Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED1A@sjhaexc02.sjha.org> Might try putting a bit of 37% formaldehyde in the bottom of a coplin and see if the vapor would fix the section enough to hold it on the slide. Good luck! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of John Baker Sent: Thursday, October 05, 2006 12:01 PM To: Histonet Subject: [Histonet] tissue coming off slides Hi All, A group I work with just came to ask if there was a way of keeping frozen tissue sections from coming off a slide? It seems there technician used uncoated/regular slides to mount 100's of sections. The tissues were embedded in OCT and cut at 5 microns but of course are sliding off during any staining procedure. Is there a protocol of any sort to coat the slides after the fact to retain the sections through staining? I at least suggested they stain flat. Any suggestions are appreciated. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From liz <@t> premierlab.com Thu Oct 5 11:45:49 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Oct 5 11:41:37 2006 Subject: [Histonet] contract labs in UK Message-ID: <000001c6e89d$b6d500c0$0300a8c0@domain.Premier> Does anyone know of contract histology lab able to perform routine H&E's. I have a client of mine that has been having difficulty getting samples in 10% NBF shipped to the US. They would need to be able to ship the completed slides back to the US. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From rjbuesa <@t> yahoo.com Thu Oct 5 12:31:44 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 12:31:54 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <20061005173144.23086.qmail@web61220.mail.yahoo.com> Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From timothy.macatee <@t> med.nyu.edu Thu Oct 5 13:04:32 2006 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Thu Oct 5 13:07:55 2006 Subject: [Histonet] Xylenes In-Reply-To: <20061003175509.97990.qmail@web61218.mail.yahoo.com> Message-ID: Hi, Has anyone had any trouble getting Xylene recently? Our in-house materials management has been out for about 2 months (they order from Fisher). I now order through Fisher and have had no problem, though. Reminds me of the Acetonitrile shortage of 1989 (do I sound like I should be in a rocking chair on the front porch?). Tim > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From rjbuesa <@t> yahoo.com Thu Oct 5 13:10:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 13:11:00 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061005181052.58492.qmail@web61213.mail.yahoo.com> Advance Magazine for Lab. Profess., Sept.25, 2006 "Morken, Tim" wrote: Rene, where did the average of 9 per lab come from? Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From rjbuesa <@t> yahoo.com Thu Oct 5 13:14:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 13:14:43 2006 Subject: [Histonet] Xylenes In-Reply-To: Message-ID: <20061005181435.54220.qmail@web61224.mail.yahoo.com> No, because benzene and aniline oil shortages were even worse in 1969! Ren? J. Timothy Macatee wrote: Hi, Has anyone had any trouble getting Xylene recently? Our in-house materials management has been out for about 2 months (they order from Fisher). I now order through Fisher and have had no problem, though. Reminds me of the Acetonitrile shortage of 1989 (do I sound like I should be in a rocking chair on the front porch?). Tim > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From PMonfils <@t> Lifespan.org Thu Oct 5 13:16:31 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Oct 5 13:16:41 2006 Subject: [Histonet] tissue coming off slides Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017177B4@lsexch.lsmaster.lifespan.org> There is the venerable old technique, seldom used today, of coating the slide with celloidin, also known as parlodion. The technique isn't used much today, partially because electrostatically charged slides have solved many of the adherence problems, and partially because the technique requires ethyl ether, which is forbidden by many institutions today because of its extreme flammability. But what we used to do is make up a solution of celloidin in 1:1 ethanol/ethyl ether, deparaffinize the slides through absolute alcohol, immerse them in the celloidin solution for about a minute, then transfer them to 80% ethanol for a few minutes to harden the celloidin film. After that they can be rehydrated and stained as usual. The celloidin forms a continuous semipermeable membrane over the entire slide and tissue, which physically holds the section in place during staining. Most dye molecules can pass through the celloidin membrane and stain the tissue. Antibody and enzyme molecules are too large to pass through the membrane, so techniques involving them cannot be done. Some standard histochemical procedures will stain the celloidin as well as the tissue, but this is not usually a problem because during the final dehydration prior to coverslipping, the xylene will dissolve away the celloidin. Many years ago we used this technique particularly for silver stains involving ammonia, since the ammonia would attack the gelatin we used as a section adhesive, and cause the sections to fall off the slides. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of John > Baker > Sent: Thursday, October 5, 2006 9:00 AM > To: Histonet > Subject: [Histonet] tissue coming off slides > > Hi All, A group I work with just came to ask if there was a way of > keeping frozen tissue sections from coming off a slide? It seems there > technician used uncoated/regular slides to mount 100's of sections. > The tissues were embedded in OCT and cut at 5 microns but of course are > sliding off during any staining procedure. Is there a protocol of any > sort to coat the slides after the fact to retain the sections through > staining? I at least suggested they stain flat. Any suggestions are > appreciated. John > > John A. Baker > The University of Michigan > Orthopaedic Research Laboratories > Histology Unit > 109 Zina Pitcher Place, 2218 BSRB > Ann Arbor, MI 48109-2200 > 734-936-1635 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From PMonfils <@t> Lifespan.org Thu Oct 5 13:20:35 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Oct 5 13:20:47 2006 Subject: [Histonet] Xylenes Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017177B5@lsexch.lsmaster.lifespan.org> Let's not forget the hematoxylin shortage in the mid-70's. Nobody had it for several months. That was a real headache! I haven't had any difficult obtaining xylene though. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Timothy Macatee > Sent: Thursday, October 5, 2006 11:04 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylenes > > > > From Kari.Zajic <@t> HCAhealthcare.com Thu Oct 5 13:28:28 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Oct 5 13:28:37 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061005173144.23086.qmail@web61220.mail.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC71A@ORLEV03.hca.corpad.net> Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 5 13:39:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 13:39:08 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC71A@ORLEV03.hca.corpad.net> Message-ID: <20061005183901.95801.qmail@web61216.mail.yahoo.com> I am glad that you are going to renew! NSH is a very prestigious society and the JOH is a first class scientific publication. Ren? J. Zajic Kari wrote: Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From Jackie.O'Connor <@t> abbott.com Thu Oct 5 13:45:34 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Oct 5 13:46:11 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC71A@ORLEV03.hca.corpad.net> Message-ID: What about those of us who are doing the work of four people? Does that count? JO'C "Zajic Kari" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2006 01:28 PM To "Rene J Buesa" , cc Subject RE: [Histonet] Histotechs: how many are we nationwide? Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DLGavin <@t> wyeth.com Thu Oct 5 13:53:13 2006 From: DLGavin <@t> wyeth.com (Donna L. Gavin) Date: Thu Oct 5 13:53:28 2006 Subject: [Histonet] In Situ Zymography Message-ID: Does anyone know of a vendor that sells pre-coated substrate slides for ISZ? I have never used this detection method and was told that I could purchase slides already coated with the specific substrate of choice. I am interested in MMP-13, MMP-9, and MMP-1. Any help will be appreciated! Thanks Donna From rjbuesa <@t> yahoo.com Thu Oct 5 13:57:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 13:58:04 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061005185756.15355.qmail@web61211.mail.yahoo.com> No, it does not count BUT if you divide your completed workload by the average you are supposed to do you will be able to demonstrate that you work for more than 1 and negotiate a proportional salary raise (just a thought!). Ren? J. Jackie M O'Connor wrote: What about those of us who are doing the work of four people? Does that count? JO'C "Zajic Kari" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2006 01:28 PM To "Rene J Buesa" , cc Subject RE: [Histonet] Histotechs: how many are we nationwide? Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From grant.steyer <@t> gmail.com Thu Oct 5 13:59:04 2006 From: grant.steyer <@t> gmail.com (Grant Steyer) Date: Thu Oct 5 13:59:13 2006 Subject: [Histonet] Reduction of Ice Crystals in Large Specimens Message-ID: <6499f4450610051159g5f856f82h15647bb8d1014765@mail.gmail.com> I routinely perform sectioning of whole mice and other large specimens. The best protocol I have found is to perfuse the mouse with a mixture of sucrose and DMSO before embedding in OCT and freezing in liquid nitrogen. Is anyone aware of other protocols that may be more effective in reducing ice crystals in large specimens? Thanks in advance for your help - Grant From christiegowan <@t> msn.com Thu Oct 5 14:25:07 2006 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Oct 5 14:25:18 2006 Subject: [Histonet] tissue coming off slides In-Reply-To: <4d17e246336fc0aaf90675b75d6110a5@umich.edu> Message-ID: We use regular slides to pick up our frozen sections when we are doing routine H&E's. We fix the slide in Rapid-Fixx(Thermo Electron Corp.) prior to staining and the sections never fall off. Good Luck! Christie Gowan >From: John Baker >To: Histonet >Subject: [Histonet] tissue coming off slides >Date: Thu, 5 Oct 2006 12:00:46 -0400 > >Hi All, A group I work with just came to ask if there was a way of keeping >frozen tissue sections from coming off a slide? It seems there technician >used uncoated/regular slides to mount 100's of sections. The tissues were >embedded in OCT and cut at 5 microns but of course are sliding off during >any staining procedure. Is there a protocol of any sort to coat the slides >after the fact to retain the sections through staining? I at least >suggested they stain flat. Any suggestions are appreciated. John > >John A. Baker >The University of Michigan >Orthopaedic Research Laboratories >Histology Unit >109 Zina Pitcher Place, 2218 BSRB >Ann Arbor, MI 48109-2200 >734-936-1635 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 5 14:27:48 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 14:27:57 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <35CF12B690D8CA4E95375A36B4E7B44C06CE13@cvm36.vetmed.wsu.edu> Message-ID: <20061005192748.47169.qmail@web61219.mail.yahoo.com> Good! The more the better! How many do you think we are? Ren? J. "Truscott, Tom" wrote: Hi Rene', Your figures might be low. There are HT's in govt. and military labs and private companies such as pharmceuticals. Tom Truscott USDA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From christiegowan <@t> msn.com Thu Oct 5 14:33:08 2006 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Oct 5 14:33:22 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061005192748.47169.qmail@web61219.mail.yahoo.com> Message-ID: There are many histotechs working for Biotech as well. Christie Gowan >From: Rene J Buesa >To: "Truscott, Tom" >,histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? >Date: Thu, 5 Oct 2006 12:27:48 -0700 (PDT) > >Good! The more the better! > How many do you think we are? > René J. > >"Truscott, Tom" wrote: > Hi Rene', Your figures might be low. There are HT's in govt. and >military labs and private companies such as pharmceuticals. Tom Truscott >USDA > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 9:32 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of them >have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership of >roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >René J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ >countries) for 2¢/min or less. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lelpers <@t> bioanalytical.com Thu Oct 5 14:41:52 2006 From: lelpers <@t> bioanalytical.com (LaDonna G. Elpers) Date: Thu Oct 5 14:42:09 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <3100BDBD96C0724D9CE9E476BFB57D962DD172@basi10003.BIOANALYTICAL.COM> Our facility has 6 Histology Technicians, my guess would be that there are probably another 15 in our town. The question would be if we talking about those certified only? We are currently half and half, 3 certified and 3 not. LaDonna G. Elpers, BA, HT(ASCP) Manager, Histology BASi (Bioanalytical Systems, Inc.) 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, October 05, 2006 1:46 PM To: Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? What about those of us who are doing the work of four people? Does that count? JO'C "Zajic Kari" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2006 01:28 PM To "Rene J Buesa" , cc Subject RE: [Histonet] Histotechs: how many are we nationwide? Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 5 14:44:23 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 14:44:35 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061005194423.86138.qmail@web61213.mail.yahoo.com> Keep adding! So, how many do you think we are? So far 49K seems to be on the "low side". Ren? J. CHRISTIE GOWAN wrote: There are many histotechs working for Biotech as well. Christie Gowan >From: Rene J Buesa >To: "Truscott, Tom" >,histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? >Date: Thu, 5 Oct 2006 12:27:48 -0700 (PDT) > >Good! The more the better! > How many do you think we are? > Ren? J. > >"Truscott, Tom" wrote: > Hi Rene', Your figures might be low. There are HT's in govt. and >military labs and private companies such as pharmceuticals. Tom Truscott >USDA > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 9:32 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of them >have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership of >roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ >countries) for 2?/min or less. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From rjbuesa <@t> yahoo.com Thu Oct 5 14:47:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 14:48:04 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <3100BDBD96C0724D9CE9E476BFB57D962DD172@basi10003.BIOANALYTICAL.COM> Message-ID: <20061005194756.54107.qmail@web61225.mail.yahoo.com> No, certification is not the issue, just how many HTs there are, how many of us share this art of histology? Ren? J. "LaDonna G. Elpers" wrote: Our facility has 6 Histology Technicians, my guess would be that there are probably another 15 in our town. The question would be if we talking about those certified only? We are currently half and half, 3 certified and 3 not. LaDonna G. Elpers, BA, HT(ASCP) Manager, Histology BASi (Bioanalytical Systems, Inc.) 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, October 05, 2006 1:46 PM To: Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? What about those of us who are doing the work of four people? Does that count? JO'C "Zajic Kari" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2006 01:28 PM To "Rene J Buesa" , cc Subject RE: [Histonet] Histotechs: how many are we nationwide? Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From Jerry <@t> ralambusa.com Thu Oct 5 14:49:20 2006 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Thu Oct 5 14:49:35 2006 Subject: [Histonet] 20. Contract Labs in UK Message-ID: <3855F92002259948A66A8CA2D16E3A4F01782D@server.ralambusa.com> Propath UK Ltd Willow Court Netherwood Road Rotherwas Industrial Estate Hereford Herefordshire HR2 6JU Tel: +44 1432 354052 I hope that helps Jerry Helisek? VP - North America 5409 Lumley Road, Unit #102 Durham, North Carolina 27703 Phone: 919-957-1964 Fax: 919-957-1972 Cell: 919-264-7964 jerry@ralambusa.com www.ralamb.com The information contained in this e-mail message may be privileged,confidential and protected from disclosure. If you are not the intendedrecipient, any dissemination, distribution or copying is strictly prohibited. Ifyou think that you have received this e-mail message in error please e-mail thesender and delete the message. Thankyou. ------------------------------ Message: 20 Date: Thu, 5 Oct 2006 10:45:49 -0600 From: "Liz Chlipala" Subject: [Histonet] contract labs in UK To: Message-ID: <000001c6e89d$b6d500c0$0300a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" Does anyone know of contract histology lab able to perform routine H&E's. I have a client of mine that has been having difficulty getting samples in 10% NBF shipped to the US. They would need to be able to ship the completed slides back to the US. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 7 *************************************** From victor <@t> pathology.washington.edu Thu Oct 5 15:07:27 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Oct 5 15:07:38 2006 Subject: [Histonet] Leica IPC Message-ID: <452565FF.8000401@pathology.washington.edu> Is anyone using the new Leica cassette labeler and printing bar-codes? -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From rjbuesa <@t> yahoo.com Thu Oct 5 15:08:44 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 5 15:08:55 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061005200844.64672.qmail@web61215.mail.yahoo.com> Good! So you think we could be as many as 60 to 70K? We are getting closer to another estimate I did for a Workshop for the FSH meeting this last May/06 of 60K that was based on the 20% of all the 292,960 "Medical and Clinical Technicians" as being HTs = 58,592! Ren? J. Surita Banwait wrote: Definitely more, we aren?t even counting the traveling temps and those working for the US oversees (volunteers in third world countries, , etc.) maybe 60-70 K -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, October 05, 2006 12:46 PM To: Surita Banwait Subject: RE: [Histonet] Histotechs: how many are we nationwide? Surita: Which is the average! So, do you think we are more than 49K or less? Ren? J. Surita Banwait wrote: There are 2 in our private research facility :) ... ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 12:28 PM To: Truscott, Tom; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Good! The more the better! How many do you think we are? Ren? J. "Truscott, Tom" wrote: Hi Rene', Your figures might be low. There are HT's in govt. and military labs and private companies such as pharmceuticals. Tom Truscott USDA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From carl.hobbs <@t> kcl.ac.uk Thu Oct 5 15:08:08 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Oct 5 15:09:09 2006 Subject: [Histonet] re:IHC on FFPE sections of Insects Message-ID: I have only carried out IHC on Drosophila embryos. I use a std FFPwE processing IHC with HIER protocol, before detection of Ag using a Std stABCpxDAB. IMHO, the fixation/processing times are not a major issue. However, THE major issue is whether or not the Abs will work on such sections! Many don?t. So, one has to then try unfixed/fixed frozen sections/brief-fixed vibratome sections. Adult insects, having chitin ?casings? will always be a problem, IMHO. I cut adult Formalin-fixed Drosophila flies on cryostat and Pwax - embedded sections for NISH and they are always problematic. Lately I have had better success using Methyl Benzoate as a clearing agent but I am not yet convinced that it is a priori better. Perhaps my techniques have improved ? You do not mention anything of the insects, tho .More details would be helpful http://www.immunoportal.com/index.php Try here..Image gallery, hit the ?search? box with ?fly? to see fly embryo IHC examples. From ander093 <@t> tc.umn.edu Thu Oct 5 15:30:09 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Thu Oct 5 15:30:27 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061005194756.54107.qmail@web61225.mail.yahoo.com> References: <3100BDBD96C0724D9CE9E476BFB57D962DD172@basi10003.BIOANALYTICAL.COM> <20061005194756.54107.qmail@web61225.mail.yahoo.com> Message-ID: <6.2.3.4.0.20061005152850.03d50da0@ander093.email.umn.edu> I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Oct 5 15:44:17 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Oct 5 15:41:13 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <6.2.3.4.0.20061005152850.03d50da0@ander093.email.umn.edu> Message-ID: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DeBrosse_Beatrice <@t> Allergan.com Thu Oct 5 16:13:09 2006 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Thu Oct 5 16:13:43 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Oct 5 16:18:36 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Oct 5 16:15:26 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Thu Oct 5 16:30:22 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Thu Oct 5 16:31:15 2006 Subject: [Histonet] tissue coming off slides Message-ID: <452516FE020000D60000BC8E@mail.mrl.ubc.ca> Paul I coat slides with celloidin for immunos here with little problem. I find some of the higher pH retrieval methods cause lose of tissue so need the coating. I just extend the time in each of the reagents by half and get nice staining. Mark Elliott iCAPTURE Centre Vancouver, BC >>> "Monfils, Paul" 10/05/06 11:16 AM >>> There is the venerable old technique, seldom used today, of coating the slide with celloidin, also known as parlodion. The technique isn't used much today, partially because electrostatically charged slides have solved many of the adherence problems, and partially because the technique requires ethyl ether, which is forbidden by many institutions today because of its extreme flammability. But what we used to do is make up a solution of celloidin in 1:1 ethanol/ethyl ether, deparaffinize the slides through absolute alcohol, immerse them in the celloidin solution for about a minute, then transfer them to 80% ethanol for a few minutes to harden the celloidin film. After that they can be rehydrated and stained as usual. The celloidin forms a continuous semipermeable membrane over the entire slide and tissue, which physically holds the section in place during staining. Most dye molecules can pass through the celloidin membrane and stain the tissue. Antibody and enzyme molecules are too large to pass through the membrane, so techniques involving them cannot be done. Some standard histochemical procedures will stain the celloidin as well as the tissue, but this is not usually a problem because during the final dehydration prior to coverslipping, the xylene will dissolve away the celloidin. Many years ago we used this technique particularly for silver stains involving ammonia, since the ammonia would attack the gelatin we used as a section adhesive, and cause the sections to fall off the slides. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of John > Baker > Sent: Thursday, October 5, 2006 9:00 AM > To: Histonet > Subject: [Histonet] tissue coming off slides > > Hi All, A group I work with just came to ask if there was a way of > keeping frozen tissue sections from coming off a slide? It seems there > technician used uncoated/regular slides to mount 100's of sections. > The tissues were embedded in OCT and cut at 5 microns but of course are > sliding off during any staining procedure. Is there a protocol of any > sort to coat the slides after the fact to retain the sections through > staining? I at least suggested they stain flat. Any suggestions are > appreciated. John > > John A. Baker > The University of Michigan > Orthopaedic Research Laboratories > Histology Unit > 109 Zina Pitcher Place, 2218 BSRB > Ann Arbor, MI 48109-2200 > 734-936-1635 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From liz <@t> premierlab.com Thu Oct 5 16:49:24 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Oct 5 16:45:13 2006 Subject: [Histonet] ISH reference Message-ID: <000001c6e8c8$1f63b850$0300a8c0@domain.Premier> Hello All Is there a good basic reference for ISH that is research based. Something that discusses the differences between gene probes, mRNA, rRNA oligo probes, the reagents that are used and why they are used. How to design probes, all of that good stuff. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From lelpers <@t> bioanalytical.com Thu Oct 5 16:53:30 2006 From: lelpers <@t> bioanalytical.com (LaDonna G. Elpers) Date: Thu Oct 5 16:53:56 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <3100BDBD96C0724D9CE9E476BFB57D962DD17D@basi10003.BIOANALYTICAL.COM> I think we may be confused here with the question of either how many technicians are there that share the love of histology versus the original question about NSH going to bat for recognition of HT's as professionals. I was present in the 2005 House when the motion was made by Catherine Locallo, seconded by Pat Patterson and adopted by the house. The motion was "I move that the National Society for Histotechnology Board of Directors form a task force to investigate how to get Histotechnician/Histotechnologist included as "Professionals" in the Federal government listing of healthcare professionals. A report to be submitted to the 2006 House of Delegate." It was understood in meetings prior to the house that there was nothing to report at this time as it is still under pursuit. Reference: 2005 House of Delegate meeting minutes from the NSH website. It would be neat to know how many person perform histology and I too am aware that persons whom are not certified are quite capable of working the bench with the certified, I witness this 5 days a week. LaDonna G. Elpers, BA, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeBrosse_Beatrice Sent: Thursday, October 05, 2006 4:13 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> MUSC.EDU Thu Oct 5 16:55:12 2006 From: dellav <@t> MUSC.EDU (Vinnie Della Speranza) Date: Thu Oct 5 16:55:41 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: I'm trying desperately to stay out of this discussion but I can help with the number you've requested. the NSH is making plans to celebrate the 20,000th HT(ASCP) which is expected to occur this Fall. I don't have #s of HTLs but it is of course much smaller. Vinnie >>> "Douglas D Deltour" 10/05/06 05:18PM >>> If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Oct 5 18:19:28 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 5 18:20:12 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <001501c6e8d4$b49ebad0$69b02e4b@HPPav2> Per ASCP Board of Registry webpage: http://www.ascp.org/Certification/ForProgramDirectors/statistics/statJanJun0 6.pdf Through June 2006: HT exam was first given in 1948 (58 years ago), and there are now 19,895 who have passed both parts of the HT exam. HTL was exam first given in 1980 (26 years ago), and there are now 2,331 who have passed both parts of the HTL exam. By way of comparison: MT - 1931 = 222,652 MTL - 1969 = 69,720 CT - 1957 = 14,244 HT - 1948 = 19,895 HTL - 1980 = 2,331 Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vinnie Della Speranza Sent: Thursday, October 05, 2006 5:55 PM To: DeBrosse_Beatrice@Allergan.com; histonet-bounces@lists.utsouthwestern.edu; doug@ppspath.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I'm trying desperately to stay out of this discussion but I can help with the number you've requested. the NSH is making plans to celebrate the 20,000th HT(ASCP) which is expected to occur this Fall. I don't have #s of HTLs but it is of course much smaller. Vinnie >>> "Douglas D Deltour" 10/05/06 05:18PM >>> If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how many HTs there are, how >many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my guess would be that >there are probably another > 15 in our town. The question would be if we talking about those >certified only? We are currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 >ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you >are taking count!! :) > >You have now inspired me to re-new my severly overdue membership with >the NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachment, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, >printing, or copying of this email or any attached files is strictly >prohibited. If you have received this email in error, please >immediately purge it and all attachments and notify the sender by reply >email or contact the sender at the number listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that >during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% >have some sort of histology lab, veterinary, marine, research, botany, >etc. = 2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) So, in universities there could be 2,000 x 2 = >4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a >membership of roughly 10% of the profession) it can be said that the >4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 6 07:35:43 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 07:35:53 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <6.2.3.4.0.20061005152850.03d50da0@ander093.email.umn.edu> Message-ID: <20061006123543.40685.qmail@web61224.mail.yahoo.com> LuAnn: Let me explain to you what I mean: positively certification is an issue in the sense that all HTs should be certified and, as a matter of fact, for potential employers certification is the first thing they consider. I was referring that for the specific topic or question of "how many are we" it is not an issue. If there are "X" number of HTs working as HTs it does not matter if they are certified or not, what matters is that they are working as HTs and are part of the total. Do I explain myself? Ren? J. LuAnn Anderson wrote: I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: No, certification is not the issue, just how many HTs there are, how many of us share this art of histology? Ren? J. "LaDonna G. Elpers" wrote: Our facility has 6 Histology Technicians, my guess would be that there are probably another 15 in our town. The question would be if we talking about those certified only? We are currently half and half, 3 certified and 3 not. LaDonna G. Elpers, BA, HT(ASCP) Manager, Histology BASi (Bioanalytical Systems, Inc.) 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, October 05, 2006 1:46 PM To: Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? What about those of us who are doing the work of four people? Does that count? JO'C "Zajic Kari" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2006 01:28 PM To "Rene J Buesa" , cc Subject RE: [Histonet] Histotechs: how many are we nationwide? Hi Rene, I enjoyed your recent article in the new Advance, was very interesting! I am only one in my facility, a general hospital. If you are taking count!! :) You have now inspired me to re-new my severly overdue membership with the NSH. Cheers from West Palm! Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 fax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From rjbuesa <@t> yahoo.com Fri Oct 6 07:41:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 07:41:10 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <02f601c6e8bd$abaca840$6701a8c0@Histopath.net> Message-ID: <20061006124102.86047.qmail@web61216.mail.yahoo.com> OK, then this would be a "downwards" correction: 1- 5,193 hospitals x 75% Texas proportion to the whole nation (assuming that this ratio is applicable nationwide) = 3900 hospitals 2- X HTs/hospital = 35,000 HTs 3- + 4,000 HTs in university settings = 39,000 HTs nationwide. A new figure to consider Now a question: those referral labs how many HTs do they have? Ren? J. Lance Beard wrote: Rene, I think your assumptions about number of hospitals with histology labs may be high. In Texas approximately 75 of the 370 general hospitals in the Texas Hospital Association have an independent lab doing their histology. -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, October 05, 2006 1:11 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Advance Magazine for Lab. Profess., Sept.25, 2006 "Morken, Tim" wrote: Rene, where did the average of 9 per lab come from? Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From rjbuesa <@t> yahoo.com Fri Oct 6 07:45:33 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 07:45:42 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <20061006124533.43791.qmail@web61224.mail.yahoo.com> And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From Kari.Zajic <@t> HCAhealthcare.com Fri Oct 6 07:45:52 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Fri Oct 6 07:46:03 2006 Subject: [Histonet] Cytology/H&E control Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC71D@ORLEV03.hca.corpad.net> Hi all, I had a manager from another facility ask me a question this morning. Her pathologist feels that there should be a "quality control slide" stained along with the cytology cases which are stained H&E. I personally have a contamination procedure in my facility to run a blank slide after cytology cases have been run to insure no cross contamination (we're CAP certified, they are JHCO) occurred. Aside from the pathologists filling out their daily QC log, what do you do in your facility? Does anyone run a QC slide with their H&E's? Is there a cytology QC slide I can direct them to make/purchase? Of course, this question is posed to me third party so I am not 100% sure on what this pathologist means, I am getting my information from a lab manager, not a histology/pathology manager... I would appreciate any feedback! Thanks in advance... Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department From rjbuesa <@t> yahoo.com Fri Oct 6 07:49:06 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 07:49:13 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <45256F97.80609@pathology.washington.edu> Message-ID: <20061006124906.15285.qmail@web61223.mail.yahoo.com> 20% of all MTs as HTs I also agree is somewhat high, that is why I tried the other approach and got to 49,000 for an average (as you point out) of roughly 1,000 per State, and that is how averages work; there will be States with more and others with less. But what about the 49,000 figure; do you thinks is too hight, too low or about right? Ren? J. Victor Tobias wrote: 20% of all MT's seems pretty high since they have staffing 24/7. I was thinking back to the 49,000. That's basically 1,000 techs per state. There are many states that don't come close to this figure and others will be over. Victor Rene J Buesa wrote: Good! So you think we could be as many as 60 to 70K? We are getting closer to another estimate I did for a Workshop for the FSH meeting this last May/06 of 60K that was based on the 20% of all the 292,960 "Medical and Clinical Technicians" as being HTs = 58,592! Ren? J. Surita Banwait wrote: Definitely more, we aren?t even counting the traveling temps and those working for the US oversees (volunteers in third world countries, , etc.) maybe 60-70 K -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, October 05, 2006 12:46 PM To: Surita Banwait Subject: RE: [Histonet] Histotechs: how many are we nationwide? Surita: Which is the average! So, do you think we are more than 49K or less? Ren? J. Surita Banwait wrote: There are 2 in our private research facility :) ... ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 12:28 PM To: Truscott, Tom; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Good! The more the better! How many do you think we are? Ren? J. "Truscott, Tom" wrote: Hi Rene', Your figures might be low. There are HT's in govt. and military labs and private companies such as pharmceuticals. Tom Truscott USDA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 05, 2006 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechs: how many are we nationwide? Dear Histonet subscriber: The US Census does NOT recognize histotechs (HTs) as a category of professionals (the NSH is going to try to do something about that during 2007) so we do not know how many we are. Some indirect calculations are needed to answer that question. According to the US Census bureau: 1- there are 5,193 general and surgical hospitals and perhaps 95% of them have a histology laboratory = 5,000 labs 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in some). So, in hospital settings there could be 5,000 x 9 = 45,000 HTs 3- There are 4,084 institutions of higher education and perhaps 50% have some sort of histology lab, veterinary, marine, research, botany, etc. = 2,000 histology labs. 4- those experimental labs have an average of 2 HTs (between 1 and 5, sometimes up to 10) So, in universities there could be 2,000 x 2 = 4,000 HTs Adding both = 49,000 histotechs nationwide. If membership to the NSH in 2006 (4,400 members) is any indicator (and usually prestigious professional societies are able to have a membership of roughly 10% of the profession) it can be said that the 4,400 members in 2006 = 9% of the proposed number. What do you think? Are we more than 49,000; less? or is 49,000 HTs about right? Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From doug <@t> ppspath.com Fri Oct 6 08:04:49 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Oct 6 08:03:22 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061006124533.43791.qmail@web61224.mail.yahoo.com> Message-ID: That was referring to the comment that LuAnn made. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 8:46 AM To: Douglas D Deltour; 'LuAnn Anderson'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 6 08:03:41 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 08:03:49 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <20061006130341.43740.qmail@web61217.mail.yahoo.com> Because the ASCP does NOT know! They only know the number of ASCP registered. Not even the CAP knows how many pathologists there are in the USA. They claim that serve 15,000 pathologists, but there have to be more than 15,000 PTs in the USA. As a matter of fact, there are 4 HTs per every 3 PTs so, if we are 49,000 there should be about 38,000 PTs. By the way, the PTs are NOT included as a special category of medical professionals in the US census; speach pathologists are, but not our PTs Ren? J. Douglas D Deltour wrote: If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From tbraud <@t> holyredeemer.com Fri Oct 6 08:04:35 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Oct 6 08:06:05 2006 Subject: [Histonet] Cytology/H&E control Message-ID: I don't know if this is enough, just right, or overkill, but we run a Formalin fixed paraffin embedded control (Section of uterus, skin, and colon) through our H&E set up every morning before staining the first rack. We run a buccal smear every morning in our FS H&E set up (just scrape our own cheeks, 95% Alc fix, then run) and we run a fixed paracentesis thin prep (used to be a cytospin) slide every morning for PAP stain control. We also run a blank slide with every FNA to check for possible cross contamination. The pathologists sign off H&E, PAP stain, and IHC stain QC sheets for overall quality. Just sharing, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory Holy Redeemer Hospital Meadowbrook, PA 19046 (215) 938-3689 From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Zajic Kari Sent: Friday, October 06, 2006 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology/H&E control Hi all, I had a manager from another facility ask me a question this morning. Her pathologist feels that there should be a "quality control slide" stained along with the cytology cases which are stained H&E. I personally have a contamination procedure in my facility to run a blank slide after cytology cases have been run to insure no cross contamination (we're CAP certified, they are JHCO) occurred. Aside from the pathologists filling out their daily QC log, what do you do in your facility? Does anyone run a QC slide with their H&E's? Is there a cytology QC slide I can direct them to make/purchase? Of course, this question is posed to me third party so I am not 100% sure on what this pathologist means, I am getting my information from a lab manager, not a histology/pathology manager... I would appreciate any feedback! Thanks in advance... Kari :) Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Fri Oct 6 08:08:46 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 08:08:56 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <3100BDBD96C0724D9CE9E476BFB57D962DD17D@basi10003.BIOANALYTICAL.COM> Message-ID: <20061006130846.94318.qmail@web61216.mail.yahoo.com> I was quoting from the NSH President's report and assuming that they were going to do something about during 2007. The thing is what you say: it would be neat to know how many of us are working in histology, how many there are nation wide doing slides daily for the PTs to do their life savings diagnosis. How many there are out there engaged in the art of histology, regardless of certification. Ren? J. "LaDonna G. Elpers" wrote: I think we may be confused here with the question of either how many technicians are there that share the love of histology versus the original question about NSH going to bat for recognition of HT's as professionals. I was present in the 2005 House when the motion was made by Catherine Locallo, seconded by Pat Patterson and adopted by the house. The motion was "I move that the National Society for Histotechnology Board of Directors form a task force to investigate how to get Histotechnician/Histotechnologist included as "Professionals" in the Federal government listing of healthcare professionals. A report to be submitted to the 2006 House of Delegate." It was understood in meetings prior to the house that there was nothing to report at this time as it is still under pursuit. Reference: 2005 House of Delegate meeting minutes from the NSH website. It would be neat to know how many person perform histology and I too am aware that persons whom are not certified are quite capable of working the bench with the certified, I witness this 5 days a week. LaDonna G. Elpers, BA, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeBrosse_Beatrice Sent: Thursday, October 05, 2006 4:13 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From rjbuesa <@t> yahoo.com Fri Oct 6 08:16:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 08:16:13 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061006131604.20765.qmail@web61218.mail.yahoo.com> Vinnie: I don't see why you are desperately trying to avoid this thread (I do not call it a discussion) if you have any solid numbers like the one on 20,000 registered HTs. It is just a simple question not about how many certified histotechs there are, but rather how many persons do the daily job of preparing slides and doing procedures that allow the diagnosis for the pathologists. Are we 49,000?; less?; more? You know this is something I have been pondering for quite some time now, as I once wrote you. And they are your potential constituency as president of the NSH! Ren? J. Vinnie Della Speranza wrote: I'm trying desperately to stay out of this discussion but I can help with the number you've requested. the NSH is making plans to celebrate the 20,000th HT(ASCP) which is expected to occur this Fall. I don't have #s of HTLs but it is of course much smaller. Vinnie >>> "Douglas D Deltour" 10/05/06 05:18PM >>> If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From rjbuesa <@t> yahoo.com Fri Oct 6 08:19:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 08:19:44 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <001501c6e8d4$b49ebad0$69b02e4b@HPPav2> Message-ID: <20061006131935.21726.qmail@web61218.mail.yahoo.com> And these colleagues ASCP certified do not even remotely account for the whole personnel doing histology work nation wide on a daily basis, and that is the number I would like to know. Ren? J. Lee & Peggy Wenk wrote: Per ASCP Board of Registry webpage: http://www.ascp.org/Certification/ForProgramDirectors/statistics/statJanJun0 6.pdf Through June 2006: HT exam was first given in 1948 (58 years ago), and there are now 19,895 who have passed both parts of the HT exam. HTL was exam first given in 1980 (26 years ago), and there are now 2,331 who have passed both parts of the HTL exam. By way of comparison: MT - 1931 = 222,652 MTL - 1969 = 69,720 CT - 1957 = 14,244 HT - 1948 = 19,895 HTL - 1980 = 2,331 Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vinnie Della Speranza Sent: Thursday, October 05, 2006 5:55 PM To: DeBrosse_Beatrice@Allergan.com; histonet-bounces@lists.utsouthwestern.edu; doug@ppspath.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I'm trying desperately to stay out of this discussion but I can help with the number you've requested. the NSH is making plans to celebrate the 20,000th HT(ASCP) which is expected to occur this Fall. I don't have #s of HTLs but it is of course much smaller. Vinnie >>> "Douglas D Deltour" 10/05/06 05:18PM >>> If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how many HTs there are, how >many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my guess would be that >there are probably another > 15 in our town. The question would be if we talking about those >certified only? We are currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 >ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you >are taking count!! :) > >You have now inspired me to re-new my severly overdue membership with >the NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachment, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, >printing, or copying of this email or any attached files is strictly >prohibited. If you have received this email in error, please >immediately purge it and all attachments and notify the sender by reply >email or contact the sender at the number listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that >during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% >have some sort of histology lab, veterinary, marine, research, botany, >etc. = 2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) So, in universities there could be 2,000 x 2 = >4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a >membership of roughly 10% of the profession) it can be said that the >4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From TownsendD <@t> childrensdayton.org Fri Oct 6 09:02:52 2006 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Oct 6 09:03:45 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: Hello, Rene I think 49,000 is on the low side. The first lab I worked for (I was not certified yet) had 15 techs, with only one certified. It was a derm lab. There are many private labs to consider, reference or otherwise, doing only Histology and employing mostly non-certified techs so they don't have to pay them as much. I think you are right when you consider non-certified to be part of the count: I have worked with many of them who were better than a lot of certified HTs. It's all a question of experience. Besides, there are so very few Histology programs that it is difficult to learn anywhere but on the job. Dolores From pmarcum <@t> vet.upenn.edu Fri Oct 6 09:04:10 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Oct 6 09:04:25 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: References: Message-ID: <6.2.5.6.2.20061006095627.01c10bf0@vet.upenn.edu> One area I don't see addressed is we may have had 20,000 registered but how many are still working. I was registered in the eight thousands in the mid seventies so the increase is not that great compared to what we seem to need now and the fact that many of us will retire over the next 5 to 10 years. The number of HTLs may increase that number however we are still woefully short of Histologist in all areas. I think actual numbers are hard to some by with all things considered. Pam Marcum At 05:55 PM 10/5/2006, Vinnie Della Speranza wrote: >I'm trying desperately to stay out of this >discussion but I can help with the number you've >requested. the NSH is making plans to celebrate >the 20,000th HT(ASCP) which is expected to occur >this Fall. I don't have #s of HTLs but it is of course much smaller. >Vinnie > > >>> "Douglas D Deltour" 10/05/06 05:18PM >>> >If that is the case then we should be able to get somewhat of an accurate >number from ASCP. I would assume??? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] >Sent: Thursday, October 05, 2006 5:10 PM >To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; >Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Maybe, but it would make the number a whole lot more accurate if only the >registered technicians would be accounted for. Otherwise, why even bother to >have a registration. > >That doesn't have anything do to with the quality of registered verses >nonregistered histotechs. I know from experience they can do just as good of >a job. But you have to draw the line somewhere. > >Beatrice DeBrosse-Serra >HT(ASCP)QIHC >Allergan, Inc. >2525 Dupont Drive RD-2A >Irvine, CA 92612 >714-246-5116 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D >Deltour >Sent: Thursday, October 05, 2006 1:44 PM >To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M >O'Connor'; 'Zajic Kari' >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Can of worms... >If you pass a NAACLS accredited school or get a degree in histology then why >wouldn't you call them a HT? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn >Anderson >Sent: Thursday, October 05, 2006 4:30 PM >To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >I think that certification should be the issue. >One does not truly have the title of HTif they >have not passed the registry exam. JMHO. > >At 02:47 PM 10/5/2006, Rene J Buesa wrote: > >No, certification is not the issue, just how > >many HTs there are, how many of us share this art of histology? > > Ren? J. > > > >"LaDonna G. Elpers" wrote: > > Our facility has 6 Histology Technicians, my > > guess would be that there are probably another > > 15 in our town. The question would be if we > > talking about those certified only? We are > > currently half and half, 3 certified and 3 not. > > > >LaDonna G. Elpers, BA, HT(ASCP) > >Manager, Histology > >BASi (Bioanalytical Systems, Inc.) > >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 > >P 812.985.5900 ext 128 > >F 812.985.3403 > >lelpers@bioanalytical.com > >www.bioanalytical.com > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > >On Behalf Of Jackie M O'Connor > >Sent: Thursday, October 05, 2006 1:46 PM > >To: Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >What about those of us who are doing the work of four people? Does that > >count? > >JO'C > > > > > > > >"Zajic Kari" > >Sent by: histonet-bounces@lists.utsouthwestern.edu > >10/05/2006 01:28 PM > > > >To > >"Rene J Buesa" , > >cc > > > >Subject > >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very > >interesting! I am only one in my facility, a general hospital. If you are > >taking count!! :) > > > >You have now inspired me to re-new my severly overdue membership with the > >NSH. > >Cheers from West Palm! > >Kari :) > > > >Kari Marie Zajic HTL,MLT > >Histology/Pathology Supervisor > >Palms West Hospital > >Pathology Department > >13001 State Road Eighty > >Loxahatchee, Florida 33470 > >phone: (561)798-6036 > >fax: (561)753-4298 > >voicemail: (561)753-4299 > >pager: (561)610-4949 > >email: Kari.Zajic@HCAHealthcare.com > > > >This email and any files transmitted with it may contain PRIVILEGED or > >CONFIDENTIAL > >information and may be read or used only by the intended recipient. If you > >are not the intended recipient of the email or any of its attachment, > >please be advised that you have received this email in error and that any > >use, dissemination, distribution, forwarding, printing, or copying of this > >email or any attached files is strictly prohibited. If you have received > >this email in error, please immediately purge it and all attachments and > >notify the sender by reply email or contact the sender at the number > >listed. > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > >Buesa > >Sent: Thursday, October 05, 2006 1:32 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Histotechs: how many are we nationwide? > > > > > >Dear Histonet subscriber: > > > >The US Census does NOT recognize histotechs (HTs) as a category of > >professionals (the NSH is going to try to do something about that during > >2007) so we do not know how many we are. Some indirect calculations are > >needed to answer that question. > >According to the US Census bureau: > > > >1- there are 5,193 general and surgical hospitals and perhaps 95% of > >them have a histology laboratory = 5,000 labs > >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in > >some). > >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > > > >3- There are 4,084 institutions of higher education and perhaps 50% have > >some sort of histology lab, veterinary, marine, research, botany, etc. = > >2,000 histology labs. > >4- those experimental labs have an average of 2 HTs (between 1 and 5, > >sometimes up to 10) > >So, in universities there could be 2,000 x 2 = 4,000 HTs > > > >Adding both = 49,000 histotechs nationwide. > > > >If membership to the NSH in 2006 (4,400 members) is any indicator (and > >usually prestigious professional societies are able to have a membership > >of roughly 10% of the profession) it can be said that the 4,400 members in > >2006 = 9% of the proposed number. > > > >What do you think? > >Are we more than 49,000; less? or is 49,000 HTs about right? > >Ren? J. > > > > > > > > > >--------------------------------- > >Do you Yahoo!? > >Get on board. You're invited to try the new Yahoo! Mail. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >--------------------------------- > >Talk is cheap. Use Yahoo! Messenger to make > >PC-to-Phone calls. Great rates starting at 1?/min. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From rjbuesa <@t> yahoo.com Fri Oct 6 09:23:37 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 09:23:46 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061006142337.41255.qmail@web61218.mail.yahoo.com> Dolores: You are so right! This is one of those "AHA!" issues: you have somebody doing a great job, efficiently and highly productive that has been paid not enough "in comparison", so why "bother" to push that fellow to get certified? To pay him or her MORE? No way! That will go against the "bottom line". At this moment there are fewer histology schools than ever and, although there are 23 "on line programs", certified schools at college level are on 11 in 9 States. I agree with you and all those who have the same "gut feeling": we are more than 49,000! Thank you for your imput! Ren? J. Dolores Townsend wrote: Hello, Rene I think 49,000 is on the low side. The first lab I worked for (I was not certified yet) had 15 techs, with only one certified. It was a derm lab. There are many private labs to consider, reference or otherwise, doing only Histology and employing mostly non-certified techs so they don't have to pay them as much. I think you are right when you consider non-certified to be part of the count: I have worked with many of them who were better than a lot of certified HTs. It's all a question of experience. Besides, there are so very few Histology programs that it is difficult to learn anywhere but on the job. Dolores --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From JWEEMS <@t> sjha.org Fri Oct 6 09:27:49 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 6 09:28:09 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED33@sjhaexc02.sjha.org> And this is exactly why NSH and others have worked so hard to require at least an associate's degree to raise the status of Histotechs. We are not viewed as professionals and will not be until we are on the same level as MLTs and MTs. My 2 more cents worth! Happy Friday Everyone, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:24 AM To: Dolores Townsend; histonet@lists.utsouthwestern.edu; ttruscot@vetmed.wsu.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Dolores: You are so right! This is one of those "AHA!" issues: you have somebody doing a great job, efficiently and highly productive that has been paid not enough "in comparison", so why "bother" to push that fellow to get certified? To pay him or her MORE? No way! That will go against the "bottom line". At this moment there are fewer histology schools than ever and, although there are 23 "on line programs", certified schools at college level are on 11 in 9 States. I agree with you and all those who have the same "gut feeling": we are more than 49,000! Thank you for your imput! Ren? J. Dolores Townsend wrote: Hello, Rene I think 49,000 is on the low side. The first lab I worked for (I was not certified yet) had 15 techs, with only one certified. It was a derm lab. There are many private labs to consider, reference or otherwise, doing only Histology and employing mostly non-certified techs so they don't have to pay them as much. I think you are right when you consider non-certified to be part of the count: I have worked with many of them who were better than a lot of certified HTs. It's all a question of experience. Besides, there are so very few Histology programs that it is difficult to learn anywhere but on the job. Dolores --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Fri Oct 6 09:31:15 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 09:31:24 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <6.2.5.6.2.20061006095627.01c10bf0@vet.upenn.edu> Message-ID: <20061006143115.68503.qmail@web61220.mail.yahoo.com> Pamela: The number is probably higher,but we should try to know, strength is in the numbers. Why we are not even considered amongst the medical professionals in the US Census? Why nobody knows how many are needed to cover the retiring HTs, those that are "baby boomers'? Why there is not a cry for more schools? Why it is allowed that some "private" labs pay functioning histotechs salaries below those of other medical lab professionals? Why is has been just now (2005) that the HTs salaries have been more or less reached the other salaries in the ML? The oficial figures of ASCP certified have to be reduced at least by half; I do not envision many HT(ASCP) certified in 1948 to be alive! And the same goes for the HTL, I am one already retired and I am not alone! Ren? J. Pamela Marcum wrote: One area I don't see addressed is we may have had 20,000 registered but how many are still working. I was registered in the eight thousands in the mid seventies so the increase is not that great compared to what we seem to need now and the fact that many of us will retire over the next 5 to 10 years. The number of HTLs may increase that number however we are still woefully short of Histologist in all areas. I think actual numbers are hard to some by with all things considered. Pam Marcum At 05:55 PM 10/5/2006, Vinnie Della Speranza wrote: >I'm trying desperately to stay out of this >discussion but I can help with the number you've >requested. the NSH is making plans to celebrate >the 20,000th HT(ASCP) which is expected to occur >this Fall. I don't have #s of HTLs but it is of course much smaller. >Vinnie > > >>> "Douglas D Deltour" 10/05/06 05:18PM >>> >If that is the case then we should be able to get somewhat of an accurate >number from ASCP. I would assume??? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] >Sent: Thursday, October 05, 2006 5:10 PM >To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; >Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Maybe, but it would make the number a whole lot more accurate if only the >registered technicians would be accounted for. Otherwise, why even bother to >have a registration. > >That doesn't have anything do to with the quality of registered verses >nonregistered histotechs. I know from experience they can do just as good of >a job. But you have to draw the line somewhere. > >Beatrice DeBrosse-Serra >HT(ASCP)QIHC >Allergan, Inc. >2525 Dupont Drive RD-2A >Irvine, CA 92612 >714-246-5116 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D >Deltour >Sent: Thursday, October 05, 2006 1:44 PM >To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M >O'Connor'; 'Zajic Kari' >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Can of worms... >If you pass a NAACLS accredited school or get a degree in histology then why >wouldn't you call them a HT? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn >Anderson >Sent: Thursday, October 05, 2006 4:30 PM >To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >I think that certification should be the issue. >One does not truly have the title of HTif they >have not passed the registry exam. JMHO. > >At 02:47 PM 10/5/2006, Rene J Buesa wrote: > >No, certification is not the issue, just how > >many HTs there are, how many of us share this art of histology? > > Ren? J. > > > >"LaDonna G. Elpers" wrote: > > Our facility has 6 Histology Technicians, my > > guess would be that there are probably another > > 15 in our town. The question would be if we > > talking about those certified only? We are > > currently half and half, 3 certified and 3 not. > > > >LaDonna G. Elpers, BA, HT(ASCP) > >Manager, Histology > >BASi (Bioanalytical Systems, Inc.) > >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 > >P 812.985.5900 ext 128 > >F 812.985.3403 > >lelpers@bioanalytical.com > >www.bioanalytical.com > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > >On Behalf Of Jackie M O'Connor > >Sent: Thursday, October 05, 2006 1:46 PM > >To: Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >What about those of us who are doing the work of four people? Does that > >count? > >JO'C > > > > > > > >"Zajic Kari" > >Sent by: histonet-bounces@lists.utsouthwestern.edu > >10/05/2006 01:28 PM > > > >To > >"Rene J Buesa" , > >cc > > > >Subject > >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very > >interesting! I am only one in my facility, a general hospital. If you are > >taking count!! :) > > > >You have now inspired me to re-new my severly overdue membership with the > >NSH. > >Cheers from West Palm! > >Kari :) > > > >Kari Marie Zajic HTL,MLT > >Histology/Pathology Supervisor > >Palms West Hospital > >Pathology Department > >13001 State Road Eighty > >Loxahatchee, Florida 33470 > >phone: (561)798-6036 > >fax: (561)753-4298 > >voicemail: (561)753-4299 > >pager: (561)610-4949 > >email: Kari.Zajic@HCAHealthcare.com > > > >This email and any files transmitted with it may contain PRIVILEGED or > >CONFIDENTIAL > >information and may be read or used only by the intended recipient. If you > >are not the intended recipient of the email or any of its attachment, > >please be advised that you have received this email in error and that any > >use, dissemination, distribution, forwarding, printing, or copying of this > >email or any attached files is strictly prohibited. If you have received > >this email in error, please immediately purge it and all attachments and > >notify the sender by reply email or contact the sender at the number > >listed. > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > >Buesa > >Sent: Thursday, October 05, 2006 1:32 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Histotechs: how many are we nationwide? > > > > > >Dear Histonet subscriber: > > > >The US Census does NOT recognize histotechs (HTs) as a category of > >professionals (the NSH is going to try to do something about that during > >2007) so we do not know how many we are. Some indirect calculations are > >needed to answer that question. > >According to the US Census bureau: > > > >1- there are 5,193 general and surgical hospitals and perhaps 95% of > >them have a histology laboratory = 5,000 labs > >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in > >some). > >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > > > >3- There are 4,084 institutions of higher education and perhaps 50% have > >some sort of histology lab, veterinary, marine, research, botany, etc. = > >2,000 histology labs. > >4- those experimental labs have an average of 2 HTs (between 1 and 5, > >sometimes up to 10) > >So, in universities there could be 2,000 x 2 = 4,000 HTs > > > >Adding both = 49,000 histotechs nationwide. > > > >If membership to the NSH in 2006 (4,400 members) is any indicator (and > >usually prestigious professional societies are able to have a membership > >of roughly 10% of the profession) it can be said that the 4,400 members in > >2006 = 9% of the proposed number. > > > >What do you think? > >Are we more than 49,000; less? or is 49,000 HTs about right? > >Ren? J. > > > > > > > > > >--------------------------------- > >Do you Yahoo!? > >Get on board. You're invited to try the new Yahoo! Mail. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >--------------------------------- > >Talk is cheap. Use Yahoo! Messenger to make > >PC-to-Phone calls. Great rates starting at 1?/min. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From dellav <@t> musc.edu Fri Oct 6 09:27:50 2006 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Oct 6 09:32:04 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: Rene, I'm trying to avoid the thread because my employer is expecting me to work and this thread will likely include a lively dialogue that may go on for some time, in part because your original question is provocative in that realistically there isn't a way to come up with anything more accurate than a guess. I've already found about fifteen messages in my inbox on this topic alone this morning. I will ask you one provocative question. are you including Mohs techs in the number you are seeking? I know Mohs techs who call themselves HT's yet their knowledge of histology is limited to cryosections of skin with H&E's. They cut beautiful sections but anything beyond skin or H&E is foreign to them. If you include them you now have countless physician practices across the country employing one or more in this capacity, most of whom are not HT certified. Vinnie >>> Rene J Buesa 10/06/06 09:16AM >>> Vinnie: I don't see why you are desperately trying to avoid this thread (I do not call it a discussion) if you have any solid numbers like the one on 20,000 registered HTs. It is just a simple question not about how many certified histotechs there are, but rather how many persons do the daily job of preparing slides and doing procedures that allow the diagnosis for the pathologists. Are we 49,000?; less?; more? You know this is something I have been pondering for quite some time now, as I once wrote you. And they are your potential constituency as president of the NSH! Ren? J. Vinnie Della Speranza wrote: I'm trying desperately to stay out of this discussion but I can help with the number you've requested. the NSH is making plans to celebrate the 20,000th HT(ASCP) which is expected to occur this Fall. I don't have #s of HTLs but it is of course much smaller. Vinnie >>> "Douglas D Deltour" 10/05/06 05:18PM >>> If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Fri Oct 6 09:33:29 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Oct 6 09:36:51 2006 Subject: [Histonet] histotechs Message-ID: At our institution we have 4 in the IHC/SS 10 in routine histology, 4 in MOHS, 2 in Pathology research, 2 in biochemistry and 1 in another research lab that I know of. There is a private lab in the area with 3, and 3 other hospitals whose numbers I don't know (maybe 10) Even with Vinnie on staff a number of our techs are not members of NSH or the state society. HE is working on making sure all are certified. It would be very interesting to see how many individuals are actually working as histologists certified or not. Rena Fail From rjbuesa <@t> yahoo.com Fri Oct 6 09:38:10 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 09:38:18 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED33@sjhaexc02.sjha.org> Message-ID: <20061006143810.70419.qmail@web61220.mail.yahoo.com> And you are right! It is right to look to the future, but if we should look to the past to gain experience, we cannot ignore the present and now there are many who are not certified and are doing a great job and providing the means for the PTs diagnosis. How many do you think we are: 49,000?; more?; less? Until we don't have an idea we cannot realize the strength of the trade. Ren? J. "Weems, Joyce" wrote: And this is exactly why NSH and others have worked so hard to require at least an associate's degree to raise the status of Histotechs. We are not viewed as professionals and will not be until we are on the same level as MLTs and MTs. My 2 more cents worth! Happy Friday Everyone, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:24 AM To: Dolores Townsend; histonet@lists.utsouthwestern.edu; ttruscot@vetmed.wsu.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Dolores: You are so right! This is one of those "AHA!" issues: you have somebody doing a great job, efficiently and highly productive that has been paid not enough "in comparison", so why "bother" to push that fellow to get certified? To pay him or her MORE? No way! That will go against the "bottom line". At this moment there are fewer histology schools than ever and, although there are 23 "on line programs", certified schools at college level are on 11 in 9 States. I agree with you and all those who have the same "gut feeling": we are more than 49,000! Thank you for your imput! Ren? J. Dolores Townsend wrote: Hello, Rene I think 49,000 is on the low side. The first lab I worked for (I was not certified yet) had 15 techs, with only one certified. It was a derm lab. There are many private labs to consider, reference or otherwise, doing only Histology and employing mostly non-certified techs so they don't have to pay them as much. I think you are right when you consider non-certified to be part of the count: I have worked with many of them who were better than a lot of certified HTs. It's all a question of experience. Besides, there are so very few Histology programs that it is difficult to learn anywhere but on the job. Dolores --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. --------------------------------- Get your email and more, right on the new Yahoo.com From akbitting <@t> geisinger.edu Fri Oct 6 09:43:16 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Oct 6 09:43:35 2006 Subject: [Histonet] Leica's auto coverslipper Message-ID: <45263344020000C9000006FE@GHSGWIANW5V.GEISINGER.EDU> We're getting 2 new Leica coverslippers this month. Can other users email me and let me know which brands of coverslips they prefer to use with this instrument? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From rjbuesa <@t> yahoo.com Fri Oct 6 09:46:42 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 09:46:50 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061006144642.96554.qmail@web61212.mail.yahoo.com> Vinnie: Those Mohs techs doing, as you say, beautiful H&E sections used in private practice diagnoses could also be included. Why not if their work is used for the diagnoses. If they are limited to H&E, many early job-trained histotech were also limited to H&E. Yes, they could also be included. Any idea on how many they are? I agree that without a solid base all these numbers are just "guesstimates" but could be useful to have at least a rough idea on our "potential" as a trade. Sorry about your "clottered" computer on the topic but you could either wait to answer later or delete them altogether (but I know you would not do it! You are too involved in your trade!). Ren? J. Vinnie Della Speranza wrote: Rene, I'm trying to avoid the thread because my employer is expecting me to work and this thread will likely include a lively dialogue that may go on for some time, in part because your original question is provocative in that realistically there isn't a way to come up with anything more accurate than a guess. I've already found about fifteen messages in my inbox on this topic alone this morning. I will ask you one provocative question. are you including Mohs techs in the number you are seeking? I know Mohs techs who call themselves HT's yet their knowledge of histology is limited to cryosections of skin with H&E's. They cut beautiful sections but anything beyond skin or H&E is foreign to them. If you include them you now have countless physician practices across the country employing one or more in this capacity, most of whom are not HT certified. Vinnie >>> Rene J Buesa 10/06/06 09:16AM >>> Vinnie: I don't see why you are desperately trying to avoid this thread (I do not call it a discussion) if you have any solid numbers like the one on 20,000 registered HTs. It is just a simple question not about how many certified histotechs there are, but rather how many persons do the daily job of preparing slides and doing procedures that allow the diagnosis for the pathologists. Are we 49,000?; less?; more? You know this is something I have been pondering for quite some time now, as I once wrote you. And they are your potential constituency as president of the NSH! Ren? J. Vinnie Della Speranza wrote: I'm trying desperately to stay out of this discussion but I can help with the number you've requested. the NSH is making plans to celebrate the 20,000th HT(ASCP) which is expected to occur this Fall. I don't have #s of HTLs but it is of course much smaller. Vinnie >>> "Douglas D Deltour" 10/05/06 05:18PM >>> If that is the case then we should be able to get somewhat of an accurate number from ASCP. I would assume??? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] Sent: Thursday, October 05, 2006 5:10 PM To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From rjbuesa <@t> yahoo.com Fri Oct 6 09:51:11 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 09:51:20 2006 Subject: [Histonet] histotechs In-Reply-To: Message-ID: <20061006145111.67247.qmail@web61215.mail.yahoo.com> Rena: You have just "pictured" a very common scenario: almost the same number are certified that are not and there are many labs not even considered. So, about my initial calculation with a "guestimate" of 49,000: do you think there are more?; less? or about right? Ren? J. Mildred Fail wrote: At our institution we have 4 in the IHC/SS 10 in routine histology, 4 in MOHS, 2 in Pathology research, 2 in biochemistry and 1 in another research lab that I know of. There is a private lab in the area with 3, and 3 other hospitals whose numbers I don't know (maybe 10) Even with Vinnie on staff a number of our techs are not members of NSH or the state society. HE is working on making sure all are certified. It would be very interesting to see how many individuals are actually working as histologists certified or not. Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Fri Oct 6 09:59:40 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 09:59:49 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061006145940.76906.qmail@web61220.mail.yahoo.com> Beatrice: That is the thing! There are people that do not have any idea what are the principles behind histology but they work as histotechs. Those who just came to embed were the ones that allowed you to section later and were part of the work flow. Had they not embedded somebody had to do it. Those who just stain H&E can be substituted by an automatic stainer or by a lab aide feeding the stainer. But as long as they do a histotech task they are facilitating the overall histology work and are part of the whole, have their fraction of the work flow from the specimen reception to the slide ready for the pathologist. Like it or not, knowingly or not, licensed or not, they are working as histotechs, even if they should not!. Ren? J. DeBrosse_Beatrice wrote: Hi Ren?, OK, ok, I guess I fell in the can of worms ..I really didn?t mean to offend anybody. I?m with you, our profession isn?t recognized or appreciated, and people do think anybody can do it. However, I worked in places where people just came in to embed, and they had no clue about anything else. Even if people stain, a lot of them follow a recipe, but do they really know how an H&E works? Oh boy, there I am again on the high horse, but there is a whole lot more to histology than ?just? embed, cut and stain. It is an art, and it is somewhat magical. I know I am taking a lot of pride in what I?m doing and I know a lot of people feel the same, registered or not. Honestly, I have no idea about the number, but it is high. I would imagine at least 49, 000. Bea -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, October 06, 2006 5:58 AM To: DeBrosse_Beatrice; Douglas D Deltour; LuAnn Anderson; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Beatrice: This is not an issue of drawing a line or anything like that: just trying to have an idea of how we all feel about this. Regardless of what should, or ought to be, the fact is that there are out there "X" number of people doing histology work; recognized or not, appreciated or not; with theoretical background or not; licensed or not. We all belong to the same trade, we all are histotechs like it or not. How many are we? 49,000? more? less? What do you think. This is just to promote a discussion on the issue of how many are we. What "respect" are entitled to as a class or trade, that is all. Not even the US Census recognizes us! Ren? J. DeBrosse_Beatrice wrote: Maybe, but it would make the number a whole lot more accurate if only the registered technicians would be accounted for. Otherwise, why even bother to have a registration. That doesn't have anything do to with the quality of registered verses nonregistered histotechs. I know from experience they can do just as good of a job. But you have to draw the line somewhere. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, October 05, 2006 1:44 PM To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M O'Connor'; 'Zajic Kari' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com >www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From liz <@t> premierlab.com Fri Oct 6 10:04:06 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 6 09:59:57 2006 Subject: [Histonet] certified techs Message-ID: <000001c6e958$ab089ca0$0300a8c0@domain.Premier> I guess I'm going to chime in here. I own a small contract lab, I have several employees on staff. I'm a certified tech and I have one other certified tech that works full time. I have two other individuals who have BS that are full time and are working there way towards taking the HTL exam. I have hired many people with degrees and train them on the job with the hopes that they will ultimately pass the registry. When I interview I want individuals who are motivated and want to take the registry. I allow them the time to study, give them whatever tools they need to help them pass the registry. I pay for their NSH membership and once they become certified I pay for their ASCP dues and the cost of the exam if they pass. As an employer I feel that this benefits me and my business. I believe that I pay well and offer the best benefits that I can because I firmly believe that you get what you pay for. As a business owner your biggest cost is your employees but they are also your biggest asset. Just my two cents - take it or leave it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From rjbuesa <@t> yahoo.com Fri Oct 6 10:03:03 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 10:03:13 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <45266AD9.3010109@pathology.washington.edu> Message-ID: <20061006150303.57774.qmail@web61219.mail.yahoo.com> Victor: It is very likely we will never know with certitude, but within some probability range (perhaps 80-90%?) we could have an idea. As long as it is not an absolutely "wild" or "completely off" number! Ren? J. Victor Tobias wrote: OK you got a few years on me. My initial reaction was the number was high, but maybe it is about right. I don't think we'll ever know. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: Now I know! The thing is that I have many more things to remember than you; I started in this trade in 1952 and I still have been able to avoid being hit by Al (Alzheimer's, I mean). But what do you think: 49,000?; more?; less? What is you "gut feeling"? Ren? J. Victor Tobias wrote: Too young!! I got my certification in the 70's by OJT. Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: Not the issue the position, just how many people do histology and can be considered histotechs. Probably you are too young to remember, but about 50 years ago in a hospital any one with dexterity could be trained on the job to prepare slides = become a histotech. Some are still around doing fantastic slides and with more experience that the average HTs. Victor Tobias wrote: Also what about your payroll/position title. Douglas D Deltour wrote: > Can of worms... > If you pass a NAACLS accredited school or get a degree in histology then why > wouldn't you call them a HT? > > > Douglas D. Deltour HT(ASCP) > Histology Supervisor > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn > Anderson > Sent: Thursday, October 05, 2006 4:30 PM > To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > I think that certification should be the issue. > One does not truly have the title of HTif they > have not passed the registry exam. JMHO. > > At 02:47 PM 10/5/2006, Rene J Buesa wrote: > >> No, certification is not the issue, just how >> many HTs there are, how many of us share this art of histology? >> Ren? J. >> >> "LaDonna G. Elpers" wrote: >> Our facility has 6 Histology Technicians, my >> guess would be that there are probably another >> 15 in our town. The question would be if we >> talking about those certified only? We are >> currently half and half, 3 certified and 3 not. >> >> LaDonna G. Elpers, BA, HT(ASCP) >> Manager, Histology >> BASi (Bioanalytical Systems, Inc.) >> 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >> P 812.985.5900 ext 128 >> F 812.985.3403 >> lelpers@bioanalytical.com >> www.bioanalytical.com >> >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Jackie M O'Connor >> Sent: Thursday, October 05, 2006 1:46 PM >> To: Zajic Kari >> Cc: histonet@lists.utsouthwestern.edu; >> histonet-bounces@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Histotechs: how many are we nationwide? >> >> What about those of us who are doing the work of four people? Does that >> count? >> JO'C >> >> >> >> "Zajic Kari" >> Sent by: histonet-bounces@lists.utsouthwestern.edu >> 10/05/2006 01:28 PM >> >> To >> "Rene J Buesa" , >> cc >> >> Subject >> RE: [Histonet] Histotechs: how many are we nationwide? >> >> >> >> >> >> >> Hi Rene, I enjoyed your recent article in the new Advance, was very >> interesting! I am only one in my facility, a general hospital. If you are >> taking count!! :) >> >> You have now inspired me to re-new my severly overdue membership with the >> NSH. >> Cheers from West Palm! >> Kari :) >> >> Kari Marie Zajic HTL,MLT >> Histology/Pathology Supervisor >> Palms West Hospital >> Pathology Department >> 13001 State Road Eighty >> Loxahatchee, Florida 33470 >> phone: (561)798-6036 >> fax: (561)753-4298 >> voicemail: (561)753-4299 >> pager: (561)610-4949 >> email: Kari.Zajic@HCAHealthcare.com >> >> This email and any files transmitted with it may contain PRIVILEGED or >> CONFIDENTIAL >> information and may be read or used only by the intended recipient. If you >> are not the intended recipient of the email or any of its attachment, >> please be advised that you have received this email in error and that any >> use, dissemination, distribution, forwarding, printing, or copying of this >> email or any attached files is strictly prohibited. If you have received >> this email in error, please immediately purge it and all attachments and >> notify the sender by reply email or contact the sender at the number >> listed. >> >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >> Buesa >> Sent: Thursday, October 05, 2006 1:32 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Histotechs: how many are we nationwide? >> >> >> Dear Histonet subscriber: >> >> The US Census does NOT recognize histotechs (HTs) as a category of >> professionals (the NSH is going to try to do something about that during >> 2007) so we do not know how many we are. Some indirect calculations are >> needed to answer that question. >> According to the US Census bureau: >> >> 1- there are 5,193 general and surgical hospitals and perhaps 95% of >> them have a histology laboratory = 5,000 labs >> 2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >> some). >> So, in hospital settings there could be 5,000 x 9 = 45,000 HTs >> >> 3- There are 4,084 institutions of higher education and perhaps 50% have >> some sort of histology lab, veterinary, marine, research, botany, etc. = >> 2,000 histology labs. >> 4- those experimental labs have an average of 2 HTs (between 1 and 5, >> sometimes up to 10) >> So, in universities there could be 2,000 x 2 = 4,000 HTs >> >> Adding both = 49,000 histotechs nationwide. >> >> If membership to the NSH in 2006 (4,400 members) is any indicator (and >> usually prestigious professional societies are able to have a membership >> of roughly 10% of the profession) it can be said that the 4,400 members in >> 2006 = 9% of the proposed number. >> >> What do you think? >> Are we more than 49,000; less? or is 49,000 HTs about right? >> Ren? J. >> >> >> >> >> --------------------------------- >> Do you Yahoo!? >> Get on board. You're invited to try the new Yahoo! Mail. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> --------------------------------- >> Talk is cheap. Use Yahoo! Messenger to make >> PC-to-Phone calls. Great rates starting at 1?/min. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Fri Oct 6 10:18:44 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 10:18:52 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <6.2.5.6.2.20061006103850.01c22780@vet.upenn.edu> Message-ID: <20061006151844.57618.qmail@web61218.mail.yahoo.com> Pamela: You are so right! I am going to transcribe to you a paragraph of an article I just finished and that is going to be published on Jan./07 titled: "Histology: a unique area of the Medical Laboratory" and the paragraphs deal with "Personnel / Staffing": "Who were the initial histotechs? They were medical students, nurses and some college graduates in disciplines unrelated to medicine or even biology. They were also anybody with manual dexterity willing to put up with toxic fumes and unsafe environments: orderlies, janitors or secretaries hoping to improve in position, as well as high school graduates and even family members and friends of the pathologists. They all just trained on the job and about 50 percent of the histology personnel were grandfathered when having a license was mandatory". O another heading I point out: "On those early days [late XIX and early XX centuries], except for some "bacteriology" pioneering research, and the microscopic observation of blood smears and urine sediments, the rest of the Medical Laboratory did not exen exist! [When histology was already a completely developed trade!]. Those facts are the ones causing the salaries disparities and the feeling some histotech have of being "looked down" by other med lab professionals. These problems will not "go away" until all are certified, have the needed theoretical knowledge and are aware of their value. A first step would be to have an idea of "hoe many we are!" Ren? J. Pamela Marcum wrote: Rene, Your questions are all on target and I don't see NSH or ASCP really answering them. The pay is low - still for most and the respect is not there. I am always amazed when I explain what histology is to someone and that we don't require a full degree I get the deer in headlights look. If lay people knew what we know about the training and expertise we are required to have and the lack of schools we might get more attention. Histology in this country started as tech being trained and staying with the same pathologist form residency to death and in the beginning it was the best we could do. Unfortuanetly the pathologist in many cases are just as guilty of not showing respect or expecting improvement or education as ASCP was when were just the ugly step children in the stinky laboratory down the hall. I am in research and have to say I have loved it more than clinical as I can get more respect and grow more than I did when I was clinical. I am thankful for the customers I worked with over the years in industry as they helped me grow in new directions also. I wish I had answers however the powers that control us are in the hands of ASCP and NSH. WE were to have more power with NSH for histology and I really don't see over my years in the field and I am a baby boomer too. Pamela At 10:31 AM 10/6/2006, you wrote: >Pamela: >The number is probably higher,but we should try >to know, strength is in the numbers. >Why we are not even considered amongst the >medical professionals in the US Census? >Why nobody knows how many are needed to cover >the retiring HTs, those that are "baby boomers'? >Why there is not a cry for more schools? >Why it is allowed that some "private" labs pay >functioning histotechs salaries below those of other medical lab professionals? >Why is has been just now (2005) that the HTs >salaries have been more or less reached the other salaries in the ML? >The oficial figures of ASCP certified have to be >reduced at least by half; I do not envision many >HT(ASCP) certified in 1948 to be alive! And the >same goes for the HTL, I am one already retired and I am not alone! >Ren? J. > >Pamela Marcum wrote: >One area I don't see addressed is we may have had >20,000 registered but how many are still >working. I was registered in the eight thousands >in the mid seventies so the increase is not that >great compared to what we seem to need now and >the fact that many of us will retire over the >next 5 to 10 years. The number of HTLs may >increase that number however we are still >woefully short of Histologist in all areas. I >think actual numbers are hard to some by with all things considered. > >Pam Marcum > >At 05:55 PM 10/5/2006, Vinnie Della Speranza wrote: > >I'm trying desperately to stay out of this > >discussion but I can help with the number you've > >requested. the NSH is making plans to celebrate > >the 20,000th HT(ASCP) which is expected to occur > >this Fall. I don't have #s of HTLs but it is of course much smaller. > >Vinnie > > > > >>> "Douglas D Deltour" 10/05/06 05:18PM >>> > >If that is the case then we should be able to get somewhat of an accurate > >number from ASCP. I would assume??? > > > > > >Douglas D. Deltour HT(ASCP) > >Histology Supervisor > >Professional Pathology Services, PC > >One Science Court > >Suite 200 > >Columbia, SC 29203 > >(803)252-1913 > >Fax (803)254-3262 > > > >***************************************************** > >PROFESSIONAL PATHOLOGY SERVICES, PC > >NOTICE OF CONFIDENTIALITY > >This message is intended only for the use of the individual or entity to > >which it is addressed and may contain information that is privileged, > >confidential and exempt from disclosure under applicable law. If the reader > >of this message is not the intended recipient, you are hereby notified that > >any dissemination, distribution, or copying of this communication is > >strictly prohibited by law. If you have received this communication in > >error, please notify me immediately. > > > >-----Original Message----- > >From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] > >Sent: Thursday, October 05, 2006 5:10 PM > >To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; > >Jackie M O'Connor; Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >Maybe, but it would make the number a whole lot more accurate if only the > >registered technicians would be accounted for. Otherwise, why even bother to > >have a registration. > > > >That doesn't have anything do to with the quality of registered verses > >nonregistered histotechs. I know from experience they can do just as good of > >a job. But you have to draw the line somewhere. > > > >Beatrice DeBrosse-Serra > >HT(ASCP)QIHC > >Allergan, Inc. > >2525 Dupont Drive RD-2A > >Irvine, CA 92612 > >714-246-5116 > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D > >Deltour > >Sent: Thursday, October 05, 2006 1:44 PM > >To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M > >O'Connor'; 'Zajic Kari' > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >Can of worms... > >If you pass a NAACLS accredited school or get a degree in histology then why > >wouldn't you call them a HT? > > > > > >Douglas D. Deltour HT(ASCP) > >Histology Supervisor > >Professional Pathology Services, PC > >One Science Court > >Suite 200 > >Columbia, SC 29203 > >(803)252-1913 > >Fax (803)254-3262 > > > >***************************************************** > >PROFESSIONAL PATHOLOGY SERVICES, PC > >NOTICE OF CONFIDENTIALITY > >This message is intended only for the use of the individual or entity to > >which it is addressed and may contain information that is privileged, > >confidential and exempt from disclosure under applicable law. If the reader > >of this message is not the intended recipient, you are hereby notified that > >any dissemination, distribution, or copying of this communication is > >strictly prohibited by law. If you have received this communication in > >error, please notify me immediately. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn > >Anderson > >Sent: Thursday, October 05, 2006 4:30 PM > >To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >I think that certification should be the issue. > >One does not truly have the title of HTif they > >have not passed the registry exam. JMHO. > > > >At 02:47 PM 10/5/2006, Rene J Buesa wrote: > > >No, certification is not the issue, just how > > >many HTs there are, how many of us share this art of histology? > > > Ren? J. > > > > > >"LaDonna G. Elpers" wrote: > > > Our facility has 6 Histology Technicians, my > > > guess would be that there are probably another > > > 15 in our town. The question would be if we > > > talking about those certified only? We are > > > currently half and half, 3 certified and 3 not. > > > > > >LaDonna G. Elpers, BA, HT(ASCP) > > >Manager, Histology > > >BASi (Bioanalytical Systems, Inc.) > > >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 > > >P 812.985.5900 ext 128 > > >F 812.985.3403 > > >lelpers@bioanalytical.com > > >www.bioanalytical.com > > > > > > > > > > > > > > >-----Original Message----- > > >From: histonet-bounces@lists.utsouthwestern.edu > > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > > >On Behalf Of Jackie M O'Connor > > >Sent: Thursday, October 05, 2006 1:46 PM > > >To: Zajic Kari > > >Cc: histonet@lists.utsouthwestern.edu; > > >histonet-bounces@lists.utsouthwestern.edu > > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > > > >What about those of us who are doing the work of four people? Does that > > >count? > > >JO'C > > > > > > > > > > > >"Zajic Kari" > > >Sent by: histonet-bounces@lists.utsouthwestern.edu > > >10/05/2006 01:28 PM > > > > > >To > > >"Rene J Buesa" , > > >cc > > > > > >Subject > > >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > > > > > > > > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very > > >interesting! I am only one in my facility, a general hospital. If you are > > >taking count!! :) > > > > > >You have now inspired me to re-new my severly overdue membership with the > > >NSH. > > >Cheers from West Palm! > > >Kari :) > > > > > >Kari Marie Zajic HTL,MLT > > >Histology/Pathology Supervisor > > >Palms West Hospital > > >Pathology Department > > >13001 State Road Eighty > > >Loxahatchee, Florida 33470 > > >phone: (561)798-6036 > > >fax: (561)753-4298 > > >voicemail: (561)753-4299 > > >pager: (561)610-4949 > > >email: Kari.Zajic@HCAHealthcare.com > > > > > >This email and any files transmitted with it may contain PRIVILEGED or > > >CONFIDENTIAL > > >information and may be read or used only by the intended recipient. If you > > >are not the intended recipient of the email or any of its attachment, > > >please be advised that you have received this email in error and that any > > >use, dissemination, distribution, forwarding, printing, or copying of this > > >email or any attached files is strictly prohibited. If you have received > > >this email in error, please immediately purge it and all attachments and > > >notify the sender by reply email or contact the sender at the number > > >listed. > > > > > > > > > > > > > > >-----Original Message----- > > >From: histonet-bounces@lists.utsouthwestern.edu > > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > > >Buesa > > >Sent: Thursday, October 05, 2006 1:32 PM > > >To: histonet@lists.utsouthwestern.edu > > >Subject: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > >Dear Histonet subscriber: > > > > > >The US Census does NOT recognize histotechs (HTs) as a category of > > >professionals (the NSH is going to try to do something about that during > > >2007) so we do not know how many we are. Some indirect calculations are > > >needed to answer that question. > > >According to the US Census bureau: > > > > > >1- there are 5,193 general and surgical hospitals and perhaps 95% of > > >them have a histology laboratory = 5,000 labs > > >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in > > >some). > > >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > > > > > >3- There are 4,084 institutions of higher education and perhaps 50% have > > >some sort of histology lab, veterinary, marine, research, botany, etc. = > > >2,000 histology labs. > > >4- those experimental labs have an average of 2 HTs (between 1 and 5, > > >sometimes up to 10) > > >So, in universities there could be 2,000 x 2 = 4,000 HTs > > > > > >Adding both = 49,000 histotechs nationwide. > > > > > >If membership to the NSH in 2006 (4,400 members) is any indicator (and > > >usually prestigious professional societies are able to have a membership > > >of roughly 10% of the profession) it can be said that the 4,400 members in > > >2006 = 9% of the proposed number. > > > > > >What do you think? > > >Are we more than 49,000; less? or is 49,000 HTs about right? > > >Ren? J. > > > > > > > > > > > > > > >--------------------------------- > > >Do you Yahoo!? > > >Get on board. You're invited to try the new Yahoo! Mail. > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > > >--------------------------------- > > >Talk is cheap. Use Yahoo! Messenger to make > > >PC-to-Phone calls. Great rates starting at 1?/min. > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Best Regards, > >Pamela A Marcum >Manager, Histology Special Procedures >University of Pennsylvania >School of Veterinary Medicine >R.S. Reynolds Jr. CORL >New Bolton Center >382 West Street Road >Kennett Square, PA 19348 > >Phone - 610-925-6278 >Fax - 610-925-8120 >E-mail - pmarcum@vet.upenn.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From ander093 <@t> tc.umn.edu Fri Oct 6 10:20:43 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri Oct 6 10:21:27 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061006124533.43791.qmail@web61224.mail.yahoo.com> References: <20061006124533.43791.qmail@web61224.mail.yahoo.com> Message-ID: <6.2.3.4.0.20061006101608.03d25c18@ander093.email.umn.edu> I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: >And who says I don't call them HTs? >For me any person doing histology work is an HT; >have you forgotten how many "trained just on the >job" HTs were grandfathered when a license was a requirement? >Is a fantastic section or a beautiful trichrome >done better by somebody accredited over somebody >with the right experience? (Another "can of worms"). >This is just a "number" of HTs nationwide = how >many of us are doing the slides on which the pathologists base their diagnosis. >Ren? J. > >Douglas D Deltour wrote: >Can of worms... >If you pass a NAACLS accredited school or get a degree in histology then why >wouldn't you call them a HT? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn >Anderson >Sent: Thursday, October 05, 2006 4:30 PM >To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >I think that certification should be the issue. >One does not truly have the title of HTif they >have not passed the registry exam. JMHO. > >At 02:47 PM 10/5/2006, Rene J Buesa wrote: > >No, certification is not the issue, just how > >many HTs there are, how many of us share this art of histology? > > Ren? J. > > > >"LaDonna G. Elpers" wrote: > > Our facility has 6 Histology Technicians, my > > guess would be that there are probably another > > 15 in our town. The question would be if we > > talking about those certified only? We are > > currently half and half, 3 certified and 3 not. > > > >LaDonna G. Elpers, BA, HT(ASCP) > >Manager, Histology > >BASi (Bioanalytical Systems, Inc.) > >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 > >P 812.985.5900 ext 128 > >F 812.985.3403 > >lelpers@bioanalytical.com > >www.bioanalytical.com > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > >On Behalf Of Jackie M O'Connor > >Sent: Thursday, October 05, 2006 1:46 PM > >To: Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >What about those of us who are doing the work of four people? Does that > >count? > >JO'C > > > > > > > >"Zajic Kari" > >Sent by: histonet-bounces@lists.utsouthwestern.edu > >10/05/2006 01:28 PM > > > >To > >"Rene J Buesa" , > >cc > > > >Subject > >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very > >interesting! I am only one in my facility, a general hospital. If you are > >taking count!! :) > > > >You have now inspired me to re-new my severly overdue membership with the > >NSH. > >Cheers from West Palm! > >Kari :) > > > >Kari Marie Zajic HTL,MLT > >Histology/Pathology Supervisor > >Palms West Hospital > >Pathology Department > >13001 State Road Eighty > >Loxahatchee, Florida 33470 > >phone: (561)798-6036 > >fax: (561)753-4298 > >voicemail: (561)753-4299 > >pager: (561)610-4949 > >email: Kari.Zajic@HCAHealthcare.com > > > >This email and any files transmitted with it may contain PRIVILEGED or > >CONFIDENTIAL > >information and may be read or used only by the intended recipient. If you > >are not the intended recipient of the email or any of its attachment, > >please be advised that you have received this email in error and that any > >use, dissemination, distribution, forwarding, printing, or copying of this > >email or any attached files is strictly prohibited. If you have received > >this email in error, please immediately purge it and all attachments and > >notify the sender by reply email or contact the sender at the number > >listed. > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > >Buesa > >Sent: Thursday, October 05, 2006 1:32 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Histotechs: how many are we nationwide? > > > > > >Dear Histonet subscriber: > > > >The US Census does NOT recognize histotechs (HTs) as a category of > >professionals (the NSH is going to try to do something about that during > >2007) so we do not know how many we are. Some indirect calculations are > >needed to answer that question. > >According to the US Census bureau: > > > >1- there are 5,193 general and surgical hospitals and perhaps 95% of > >them have a histology laboratory = 5,000 labs > >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in > >some). > >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > > > >3- There are 4,084 institutions of higher education and perhaps 50% have > >some sort of histology lab, veterinary, marine, research, botany, etc. = > >2,000 histology labs. > >4- those experimental labs have an average of 2 HTs (between 1 and 5, > >sometimes up to 10) > >So, in universities there could be 2,000 x 2 = 4,000 HTs > > > >Adding both = 49,000 histotechs nationwide. > > > >If membership to the NSH in 2006 (4,400 members) is any indicator (and > >usually prestigious professional societies are able to have a membership > >of roughly 10% of the profession) it can be said that the 4,400 members in > >2006 = 9% of the proposed number. > > > >What do you think? > >Are we more than 49,000; less? or is 49,000 HTs about right? > >Ren? J. > > > > > > > > > >--------------------------------- > >Do you Yahoo!? > >Get on board. You're invited to try the new Yahoo! Mail. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >--------------------------------- > >Talk is cheap. Use Yahoo! Messenger to make > >PC-to-Phone calls. Great rates starting at 1?/min. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > >Do you Yahoo!? >Get on board. >You're >invited to try the new Yahoo! Mail. From rjbuesa <@t> yahoo.com Fri Oct 6 10:28:02 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 10:28:10 2006 Subject: [Histonet] certified techs In-Reply-To: <000001c6e958$ab089ca0$0300a8c0@domain.Premier> Message-ID: <20061006152803.86300.qmail@web61220.mail.yahoo.com> Liz: And you are doing the right thing but not everybody does that! Fairness in employment is usually not a quality of some employers that are only looking at the profit and thinking that if somebody goes, another will replace it. Histology is an unique area in the medical lab and our samples are essentially solid, discrete, unique and mostly irreplaceable! Sample lost is lost for ever! Section not used is lost also for ever! Training is fundamental in our trade and the histotech is constantly making decisions: how much tissue per cassette; what surface to embed to section; when the decal is ready; when to renew the reagents; when the end point was reached in the staining method; constantly taking decisions that can impact on the specimen and, consequently, on the diagnosis. Training is essential and getting a license helps with the knowledge required for that decision making but this is sometimes overlooked and not encouraged. Ren? J. Liz Chlipala wrote: I guess I'm going to chime in here. I own a small contract lab, I have several employees on staff. I'm a certified tech and I have one other certified tech that works full time. I have two other individuals who have BS that are full time and are working there way towards taking the HTL exam. I have hired many people with degrees and train them on the job with the hopes that they will ultimately pass the registry. When I interview I want individuals who are motivated and want to take the registry. I allow them the time to study, give them whatever tools they need to help them pass the registry. I pay for their NSH membership and once they become certified I pay for their ASCP dues and the cost of the exam if they pass. As an employer I feel that this benefits me and my business. I believe that I pay well and offer the best benefits that I can because I firmly believe that you get what you pay for. As a business owner your biggest cost is your employees but they are also your biggest asset. Just my two cents - take it or leave it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From rjbuesa <@t> yahoo.com Fri Oct 6 10:36:10 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 10:36:21 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <6.2.3.4.0.20061006101608.03d25c18@ander093.email.umn.edu> Message-ID: <20061006153610.93296.qmail@web61224.mail.yahoo.com> LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From TownsendD <@t> childrensdayton.org Fri Oct 6 10:43:14 2006 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Oct 6 10:44:18 2006 Subject: [Histonet] histotechs Message-ID: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores From ander093 <@t> tc.umn.edu Fri Oct 6 10:51:53 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri Oct 6 10:52:12 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061006153610.93296.qmail@web61224.mail.yahoo.com> References: <6.2.3.4.0.20061006101608.03d25c18@ander093.email.umn.edu> <20061006153610.93296.qmail@web61224.mail.yahoo.com> Message-ID: <6.2.3.4.0.20061006105111.01db4110@ander093.email.umn.edu> Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: >LuAnn: >I write "HT" because it has less letters than >writing "histotech", no reference to the >"Histotechnician" ASCP title. It is just easier to type! >But again: with that in mind: what do you think: are we 49,000?; less?' more? >Ren? J. > >LuAnn Anderson wrote: >I never said nor did I imply that people who are >not certified are not capable of doing a good >job. I know very well that they can--I have >trained many myself. I simply said that if you >are asking for the number of "HT's" then you >would need to be counting those certified--just >because one is doing histology does not give >them the title of HT--technically one must pass >the registry to have that title. If you are just >looking for the total number of people who work >in histology labs--then that is a different question all together.~~LuAnn > > > >At 07:45 AM 10/6/2006, Rene J Buesa wrote: >>And who says I don't call them HTs? >>For me any person doing histology work is an >>HT; have you forgotten how many "trained just >>on the job" HTs were grandfathered when a license was a requirement? >>Is a fantastic section or a beautiful trichrome >>done better by somebody accredited over >>somebody with the right experience? (Another "can of worms"). >>This is just a "number" of HTs nationwide = how >>many of us are doing the slides on which the pathologists base their diagnosis. >>Ren? J. >> >>Douglas D Deltour wrote: >>Can of worms... >>If you pass a NAACLS accredited school or get a degree in histology then why >>wouldn't you call them a HT? >> >>Douglas D. Deltour HT(ASCP) >>Histology Supervisor >>Professional Pathology Services, PC >>One Science Court >>Suite 200 >>Columbia, SC 29203 >>(803)252-1913 >>Fax (803)254-3262 >>***************************************************** >>PROFESSIONAL PATHOLOGY SERVICES, PC >>NOTICE OF CONFIDENTIALITY >>This message is intended only for the use of the individual or entity to >>which it is addressed and may contain information that is privileged, >>confidential and exempt from disclosure under applicable law. If the reader >>of this message is not the intended recipient, you are hereby notified that >>any dissemination, distribution, or copying of this communication is >>strictly prohibited by law. If you have received this communication in >>error, please notify me immediately. >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn >>Anderson >>Sent: Thursday, October 05, 2006 4:30 PM >>To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari >>Cc: histonet@lists.utsouthwestern.edu; >>histonet-bounces@lists.utsouthwestern.edu >>Subject: RE: [Histonet] Histotechs: how many are we nationwide? >>I think that certification should be the issue. >>One does not truly have the title of HTif they >>have not passed the registry exam. JMHO. >>At 02:47 PM 10/5/2006, Rene J Buesa wrote: >> >No, certification is not the issue, just how >> >many HTs there are, how many of us share this art of histology? >> > Ren? J. >> > >> >"LaDonna G. Elpers" wrote: >> > Our facility has 6 Histology Technicians, my >> > guess would be that there are probably another >> > 15 in our town. The question would be if we >> > talking about those certified only? We are >> > currently half and half, 3 certified and 3 not. >> > >> >LaDonna G. Elpers, BA, HT(ASCP) >> >Manager, Histology >> >BASi (Bioanalytical Systems, Inc.) >> >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >> >P 812.985.5900 ext 128 >> >F 812.985.3403 >> >lelpers@bioanalytical.com >> > www.bioanalytical.com >> > >> > >> > >> > >> >-----Original Message----- >> >From: histonet-bounces@lists.utsouthwestern.edu >> >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >> >On Behalf Of Jackie M O'Connor >> >Sent: Thursday, October 05, 2006 1:46 PM >> >To: Zajic Kari >> >Cc: histonet@lists.utsouthwestern.edu; >> >histonet-bounces@lists.utsouthwestern.edu >> >Subject: RE: [Histonet] Histotechs: how many are we nationwide? >> > >> >What about those of us who are doing the work of four people? Does that >> >count? >> >JO'C >> > >> > >> > >> >"Zajic Kari" >> >Sent by: histonet-bounces@lists.utsouthwestern.edu >> >10/05/2006 01:28 PM >> > >> >To >> >"Rene J Buesa" , >> >cc >> > >> >Subject >> >RE: [Histonet] Histotechs: how many are we nationwide? >> > >> > >> > >> > >> > >> > >> >Hi Rene, I enjoyed your recent article in the new Advance, was very >> >interesting! I am only one in my facility, a general hospital. If you are >> >taking count!! :) >> > >> >You have now inspired me to re-new my severly overdue membership with the >> >NSH. >> >Cheers from West Palm! >> >Kari :) >> > >> >Kari Marie Zajic HTL,MLT >> >Histology/Pathology Supervisor >> >Palms West Hospital >> >Pathology Department >> >13001 State Road Eighty >> >Loxahatchee, Florida 33470 >> >phone: (561)798-6036 >> >fax: (561)753-4298 >> >voicemail: (561)753-4299 >> >pager: (561)610-4949 >> >email: Kari.Zajic@HCAHealthcare.com >> > >> >This email and any files transmitted with it may contain PRIVILEGED or >> >CONFIDENTIAL >> >information and may be read or used only by the intended recipient. If you >> >are not the intended recipient of the email or any of its attachment, >> >please be advised that you have received this email in error and that any >> >use, dissemination, distribution, forwarding, printing, or copying of this >> >email or any attached files is strictly prohibited. If you have received >> >this email in error, please immediately purge it and all attachments and >> >notify the sender by reply email or contact the sender at the number >> >listed. >> > >> > >> > >> > >> >-----Original Message----- >> >From: histonet-bounces@lists.utsouthwestern.edu >> >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >> >Buesa >> >Sent: Thursday, October 05, 2006 1:32 PM >> >To: histonet@lists.utsouthwestern.edu >> >Subject: [Histonet] Histotechs: how many are we nationwide? >> > >> > >> >Dear Histonet subscriber: >> > >> >The US Census does NOT recognize histotechs (HTs) as a category of >> >professionals (the NSH is going to try to do something about that during >> >2007) so we do not know how many we are. Some indirect calculations are >> >needed to answer that question. >> >According to the US Census bureau: >> > >> >1- there are 5,193 general and surgical hospitals and perhaps 95% of >> >them have a histology laboratory = 5,000 labs >> >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >> >some). >> >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs >> > >> >3- There are 4,084 institutions of higher education and perhaps 50% have >> >some sort of histology lab, veterinary, marine, research, botany, etc. = >> >2,000 histology labs. >> >4- those experimental labs have an average of 2 HTs (between 1 and 5, >> >sometimes up to 10) >> >So, in universities there could be 2,000 x 2 = 4,000 HTs >> > >> >Adding both = 49,000 histotechs nationwide. >> > >> >If membership to the NSH in 2006 (4,400 members) is any indicator (and >> >usually prestigious professional societies are able to have a membership >> >of roughly 10% of the profession) it can be said that the 4,400 members in >> >2006 = 9% of the proposed number. >> > >> >What do you think? >> >Are we more than 49,000; less? or is 49,000 HTs about right? >> >Ren? J. >> > >> > >> > >> > >> >--------------------------------- >> >Do you Yahoo!? >> >Get on board. You're invited to try the new Yahoo! Mail. >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > >> > >> > >> > >> >--------------------------------- >> >Talk is cheap. Use Yahoo! Messenger to make >> >PC-to-Phone calls. Great rates starting at 1?/min. >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >>Do you Yahoo!? >>Get on board. >>You're >>invited to try the new Yahoo! Mail. > > > > > >Stay in the know. Pulse on the new Yahoo.com. >Check it out. From rjbuesa <@t> yahoo.com Fri Oct 6 11:00:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 11:00:26 2006 Subject: [Histonet] histotechs In-Reply-To: Message-ID: <20061006160018.996.qmail@web61224.mail.yahoo.com> Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com From rjbuesa <@t> yahoo.com Fri Oct 6 11:19:19 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 11:19:28 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <6.2.3.4.0.20061006105111.01db4110@ander093.email.umn.edu> Message-ID: <20061006161919.71000.qmail@web61211.mail.yahoo.com> Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From eli4trac <@t> aol.com Fri Oct 6 11:22:13 2006 From: eli4trac <@t> aol.com (eli4trac@aol.com) Date: Fri Oct 6 11:22:30 2006 Subject: [Histonet] take me off of listserver Message-ID: <8C8B77AB78A0067-172C-442E@FWM-M44.sysops.aol.com> I would like to be taken off of listserver, I signed up for one class only, now that class is over. ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From cormier <@t> MIT.EDU Fri Oct 6 11:29:45 2006 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Fri Oct 6 11:30:07 2006 Subject: [Histonet] phalloidin? Message-ID: <001e01c6e964$a2d5a670$92003712@mit.edu> Hello 'Netters, Does anyone know of any phalloidin replacement that works as nicely as phalloidin, but safer to work with? (one never knows right?) We would be using this on frozen mouse sections... Thanks and have a great long weekend! Kathy From pruegg <@t> ihctech.net Fri Oct 6 11:48:35 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Oct 6 11:48:49 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <20061006161919.71000.qmail@web61211.mail.yahoo.com> Message-ID: <006001c6e967$464777f0$6601a8c0@Patsy> Rene, I have never heard of anyone being "grandfathered" in as an HT. Perhaps you are speaking of when the HTL certification first began around 1980? At that time if you were a certified HT with so many years of experience (?10) you could be grandfathered in as an HTL without having the required BS degree, but I believe you still had to take the HTL exam. ASCP certifies HT/HTL's and they do have an eligibility route which may include OJT but everyone has to take the exam to be called an HT. I know there are plenty of people working and doing a good job who do not have the certification from ASCP, so why don't they just try to take the exam and get the certification? Allowing anyone doing histology work to be called an HT would I believe diminish the significance of those of us who have bothered to be recognized as professionally certified by ASCP. Medical technology is a field that is regulated requiring that those in laboratory medicine be certified to do the test for patients that matter so much in their health care. Would you want just anybody performing such tests on you or your loved ones? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:19 AM To: LuAnn Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cormier <@t> MIT.EDU Fri Oct 6 11:49:09 2006 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Fri Oct 6 11:49:30 2006 Subject: [Histonet] phalloidin? References: <100620061633.354.45268558000DF519000001622160280741050E960C9D9A0606@att.net> Message-ID: <001201c6e967$589d3980$92003712@mit.edu> I just found out what is was last week, so I know what you are thinking...We just used it to stain filamentous actin in our tissue sections. We had a native population of cells that autofluoresced, and wanted to get "fluorescent H and E" to go along w/ it. It worked quite nicely, but it is really really toxic. Kathy ----- Original Message ----- From: jjurczak@att.net To: Kathy Cormier Sent: Friday, October 06, 2006 12:33 PM Subject: Re: [Histonet] phalloidin? Excuse my ignorance, but what is pahlloidin and what do you do with it? -------------- Original message from "Kathy Cormier" : -------------- > Hello 'Netters, > > Does anyone know of any phalloidin replacement that works as nicely as > phalloidin, but safer to work with? (one never knows right?) We would be using > this on frozen mouse sections... > > Thanks and have a great long weekend! > > Kathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Oct 6 11:52:39 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Oct 6 11:52:56 2006 Subject: [Histonet] histotechs In-Reply-To: <20061006160018.996.qmail@web61224.mail.yahoo.com> Message-ID: <006101c6e967$d66b7e30$6601a8c0@Patsy> You do not have to pay dues to ASCP to stay registered as an HT. The one time exam and certification is for life. Once you are certified by ASCP you can always call yourself an HT. They have initiated a continuing education requirement for those new HT's taking their certification, now you must submit proof of CEU's to maintain your certification. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:00 AM To: Dolores Townsend; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: Re: [Histonet] histotechs Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Oct 6 11:52:59 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 6 11:53:17 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED44@sjhaexc02.sjha.org> And you all must know that we now should be saying "Grandparented" in - instead of Grandfathered... a bit of Friday humor (very light I know) j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Friday, October 06, 2006 12:49 PM To: 'Rene J Buesa'; 'LuAnn Anderson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Rene, I have never heard of anyone being "grandfathered" in as an HT. Perhaps you are speaking of when the HTL certification first began around 1980? At that time if you were a certified HT with so many years of experience (?10) you could be grandfathered in as an HTL without having the required BS degree, but I believe you still had to take the HTL exam. ASCP certifies HT/HTL's and they do have an eligibility route which may include OJT but everyone has to take the exam to be called an HT. I know there are plenty of people working and doing a good job who do not have the certification from ASCP, so why don't they just try to take the exam and get the certification? Allowing anyone doing histology work to be called an HT would I believe diminish the significance of those of us who have bothered to be recognized as professionally certified by ASCP. Medical technology is a field that is regulated requiring that those in laboratory medicine be certified to do the test for patients that matter so much in their health care. Would you want just anybody performing such tests on you or your loved ones? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:19 AM To: LuAnn Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Fri Oct 6 11:53:51 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 6 11:54:15 2006 Subject: [Histonet] phalloidin? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED45@sjhaexc02.sjha.org> Thank you so much. I just googled and was trying to wade through all that stuff!!! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kathy Cormier Sent: Friday, October 06, 2006 12:49 PM To: jjurczak@att.net Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] phalloidin? I just found out what is was last week, so I know what you are thinking...We just used it to stain filamentous actin in our tissue sections. We had a native population of cells that autofluoresced, and wanted to get "fluorescent H and E" to go along w/ it. It worked quite nicely, but it is really really toxic. Kathy ----- Original Message ----- From: jjurczak@att.net To: Kathy Cormier Sent: Friday, October 06, 2006 12:33 PM Subject: Re: [Histonet] phalloidin? Excuse my ignorance, but what is pahlloidin and what do you do with it? -------------- Original message from "Kathy Cormier" : -------------- > Hello 'Netters, > > Does anyone know of any phalloidin replacement that works as nicely as > phalloidin, but safer to work with? (one never knows right?) We would be using > this on frozen mouse sections... > > Thanks and have a great long weekend! > > Kathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Fri Oct 6 11:56:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 11:56:46 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: Message-ID: <20061006165635.73765.qmail@web61214.mail.yahoo.com> Tim: I could not have said it better (even if that is really easy, I mean, saying something better than me!). Now since I get very focused (at times and while I am free from Al's grip; Alzheimer's I mean): I have to understand that without much difficulty we could be more than the 49,000 I "guestimated"? Ren? J. "Morken, Tim" wrote: Rene, The answer to several of your questions has to do with how a histotech fits in the medical field. The salient fact is that the histotech does not make any diagnostic decisions. That is the province of the pathologist. On the other hand, med techs are "signing out" cases all the time in the "clinical" lab and the pathologist only is involved when there is a discrepancy (sometimes not even then). This happens because med techs are for the most part reporting quantitiative values taken from their instruments. In microbiology they are reporting out postive/negative infections. Cytologists are reporting out diagnoses on interpreted information - far beyond most med tech work - and they have legal liability for it. Because of this diagnostic responsibility the med tech and cytotech has a much higher profile in the medical field than histotechs. Histotechs do the work, pathologists take all the glory (such as it is!). The histotech, in the US at least, is almost always an on-the-job trained person from a background that is probably not medical, or even biology related. The historical baggage has led to the field being seen by most as a backwater even today. Of course we on Histonet know that histotechnology has come a long, long way since the days when all we did was h&e's and special stains. I realized how far when pathologists began asking me which antibodies we should run on various cases. I knew more than some of them! Histotechnolgy is the equal of any other medical lab field in terms of technology and relevance, but it is still not a diagnostic job. We provide information that must be interpreted, and unfortunately (for histotechs) in most cases it requires great expertise to interpret. I am actually quite amazed that histotechs can now demand the same pay as med techs in most places, and in some places even more. Indeed, in my home area, the San Francisco Bay Area, a histotech with only a few years of experience can demand 50K to 70K for only basic histology work (not IHC, that pays even more!) - and labs will fight over them. That is far different than 25 years ago. The fact some labs hire unskilled people and train on the job is a fact of life. As noted, there are few schools for it, and only a handful of students will ever even hear about the profession (thus my appearance in a high school textbook). Even if a student did hear about histotechnology, how would they get into it without extensive help? Every single lab I have visited wants more techs but cannot find any. Since they are allowed to use unskilled people, and train them, they do (and pay much less - no qualifications, no degree, no certification, why pay more?). The only way that will not happen is if hundreds of schools are opened. Who will teach at these schools? The only people qualified to do so are working in the field. How many of those want to teach? I've considered it but it is a big job so who knows.... So how may histotechs? A LOT more than the numbers suggest. The vast majority are not certified, do not go to meetings and never hear anything about such things. I'm not sure anyone has every tried to even figure that out (besides Rene!). Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 7:31 AM To: Pamela Marcum; Vinnie Della Speranza; DeBrosse_Beatrice@Allergan.com; histonet-bounces@lists.utsouthwestern.edu; doug@ppspath.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Pamela: The number is probably higher,but we should try to know, strength is in the numbers. Why we are not even considered amongst the medical professionals in the US Census? Why nobody knows how many are needed to cover the retiring HTs, those that are "baby boomers'? Why there is not a cry for more schools? Why it is allowed that some "private" labs pay functioning histotechs salaries below those of other medical lab professionals? Why is has been just now (2005) that the HTs salaries have been more or less reached the other salaries in the ML? The oficial figures of ASCP certified have to be reduced at least by half; I do not envision many HT(ASCP) certified in 1948 to be alive! And the same goes for the HTL, I am one already retired and I am not alone! Ren? J. Pamela Marcum wrote: One area I don't see addressed is we may have had 20,000 registered but how many are still working. I was registered in the eight thousands in the mid seventies so the increase is not that great compared to what we seem to need now and the fact that many of us will retire over the next 5 to 10 years. The number of HTLs may increase that number however we are still woefully short of Histologist in all areas. I think actual numbers are hard to some by with all things considered. Pam Marcum At 05:55 PM 10/5/2006, Vinnie Della Speranza wrote: >I'm trying desperately to stay out of this discussion but I can help >with the number you've requested. the NSH is making plans to celebrate >the 20,000th HT(ASCP) which is expected to occur this Fall. I don't >have #s of HTLs but it is of course much smaller. >Vinnie > > >>> "Douglas D Deltour" 10/05/06 05:18PM >>> >If that is the case then we should be able to get somewhat of an >accurate number from ASCP. I would assume??? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity >to which it is addressed and may contain information that is >privileged, confidential and exempt from disclosure under applicable >law. If the reader of this message is not the intended recipient, you >are hereby notified that any dissemination, distribution, or copying of >this communication is strictly prohibited by law. If you have received >this communication in error, please notify me immediately. > >-----Original Message----- >From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] >Sent: Thursday, October 05, 2006 5:10 PM >To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; >Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Maybe, but it would make the number a whole lot more accurate if only >the registered technicians would be accounted for. Otherwise, why even >bother to have a registration. > >That doesn't have anything do to with the quality of registered verses >nonregistered histotechs. I know from experience they can do just as >good of a job. But you have to draw the line somewhere. > >Beatrice DeBrosse-Serra >HT(ASCP)QIHC >Allergan, Inc. >2525 Dupont Drive RD-2A >Irvine, CA 92612 >714-246-5116 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >D Deltour >Sent: Thursday, October 05, 2006 1:44 PM >To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M >O'Connor'; 'Zajic Kari' >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Can of worms... >If you pass a NAACLS accredited school or get a degree in histology >then why wouldn't you call them a HT? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity >to which it is addressed and may contain information that is >privileged, confidential and exempt from disclosure under applicable >law. If the reader of this message is not the intended recipient, you >are hereby notified that any dissemination, distribution, or copying of >this communication is strictly prohibited by law. If you have received >this communication in error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn >Anderson >Sent: Thursday, October 05, 2006 4:30 PM >To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >I think that certification should be the issue. >One does not truly have the title of HTif they have not passed the >registry exam. JMHO. > >At 02:47 PM 10/5/2006, Rene J Buesa wrote: > >No, certification is not the issue, just how many HTs there are, how > >many of us share this art of histology? > > Ren? J. > > > >"LaDonna G. Elpers" wrote: > > Our facility has 6 Histology Technicians, my guess would be that > >there are probably another > > 15 in our town. The question would be if we talking about those > >certified only? We are currently half and half, 3 certified and 3 > >not. > > > >LaDonna G. Elpers, BA, HT(ASCP) > >Manager, Histology > >BASi (Bioanalytical Systems, Inc.) > >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 > >ext 128 F 812.985.3403 lelpers@bioanalytical.com > >www.bioanalytical.com > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > >On Behalf Of Jackie M O'Connor > >Sent: Thursday, October 05, 2006 1:46 PM > >To: Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >What about those of us who are doing the work of four people? Does that > >count? > >JO'C > > > > > > > >"Zajic Kari" > >Sent by: histonet-bounces@lists.utsouthwestern.edu > >10/05/2006 01:28 PM > > > >To > >"Rene J Buesa" , > >cc > > > >Subject > >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very > >interesting! I am only one in my facility, a general hospital. If you are > >taking count!! :) > > > >You have now inspired me to re-new my severly overdue membership with the > >NSH. > >Cheers from West Palm! > >Kari :) > > > >Kari Marie Zajic HTL,MLT > >Histology/Pathology Supervisor > >Palms West Hospital > >Pathology Department > >13001 State Road Eighty > >Loxahatchee, Florida 33470 > >phone: (561)798-6036 > >fax: (561)753-4298 > >voicemail: (561)753-4299 > >pager: (561)610-4949 > >email: Kari.Zajic@HCAHealthcare.com > > > >This email and any files transmitted with it may contain PRIVILEGED or > >CONFIDENTIAL > >information and may be read or used only by the intended recipient. If you > >are not the intended recipient of the email or any of its attachment, > >please be advised that you have received this email in error and that any > >use, dissemination, distribution, forwarding, printing, or copying of this > >email or any attached files is strictly prohibited. If you have received > >this email in error, please immediately purge it and all attachments and > >notify the sender by reply email or contact the sender at the number > >listed. > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > >Buesa > >Sent: Thursday, October 05, 2006 1:32 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Histotechs: how many are we nationwide? > > > > > >Dear Histonet subscriber: > > > >The US Census does NOT recognize histotechs (HTs) as a category of > >professionals (the NSH is going to try to do something about that during > >2007) so we do not know how many we are. Some indirect calculations are > >needed to answer that question. > >According to the US Census bureau: > > > >1- there are 5,193 general and surgical hospitals and perhaps 95% of > >them have a histology laboratory = 5,000 labs > >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in > >some). > >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > > > >3- There are 4,084 institutions of higher education and perhaps 50% have > >some sort of histology lab, veterinary, marine, research, botany, etc. = > >2,000 histology labs. > >4- those experimental labs have an average of 2 HTs (between 1 and 5, > >sometimes up to 10) > >So, in universities there could be 2,000 x 2 = 4,000 HTs > > > >Adding both = 49,000 histotechs nationwide. > > > >If membership to the NSH in 2006 (4,400 members) is any indicator (and > >usually prestigious professional societies are able to have a membership > >of roughly 10% of the profession) it can be said that the 4,400 members in > >2006 = 9% of the proposed number. > > > >What do you think? > >Are we more than 49,000; less? or is 49,000 HTs about right? > >Ren? J. > > > > > > > > > >--------------------------------- > >Do you Yahoo!? > >Get on board. You're invited to try the new Yahoo! Mail. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >--------------------------------- > >Talk is cheap. Use Yahoo! Messenger to make > >PC-to-Phone calls. Great rates starting at 1?/min. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From GDawson <@t> dynacaremilwaukee.com Fri Oct 6 11:58:25 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Oct 6 11:58:34 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: Sounds more difficult than trying to take conduct a census since unregistered techs would be like trying to find all the homeless that could be in the woods, under a bridge, etc... Also, lets be wary about putting histotechs into the same bucket as artists since most artist are called "struggling" for a reason & histotechs have bills to pay. Put your hackles down, just some poor Friday humor. Glen Dawson Milwaukee, WI From rjbuesa <@t> yahoo.com Fri Oct 6 12:02:37 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 12:02:46 2006 Subject: Fwd: RE: [Histonet] Histotechs: how many are we nationwide? Message-ID: <20061006170238.75630.qmail@web61214.mail.yahoo.com> Rene J Buesa wrote: Date: Fri, 6 Oct 2006 10:01:21 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Histotechs: how many are we nationwide? To: Patsy Ruegg Patsy: I use "HT" as an abbreviation for "histotech" wehich is easier to write. Any State, with licensure requirements, "grandfathered" those working in histology that were required by the State to have a State licensure to work. That is the "grandfathering" I am referring to. Those States that even today do not require their HTs to have a State license did not have to do that. I am not writing about the ASCP HT licensure! But to the question: do you think we are 49,000?; more?; less? Ren? J. Patsy Ruegg wrote: Rene, I have never heard of anyone being "grandfathered" in as an HT. Perhaps you are speaking of when the HTL certification first began around 1980? At that time if you were a certified HT with so many years of experience (?10) you could be grandfathered in as an HTL without having the required BS degree, but I believe you still had to take the HTL exam. ASCP certifies HT/HTL's and they do have an eligibility route which may include OJT but everyone has to take the exam to be called an HT. I know there are plenty of people working and doing a good job who do not have the certification from ASCP, so why don't they just try to take the exam and get the certification? Allowing anyone doing histology work to be called an HT would I believe diminish the significance of those of us who have bothered to be recognized as professionally certified by ASCP. Medical technology is a field that is regulated requiring that those in laboratory medicine be certified to do the test for patients that matter so much in their health care. Would you want just anybody performing such tests on you or your loved ones? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:19 AM To: LuAnn Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From Virginia.Achstetter <@t> afip.osd.mil Fri Oct 6 12:06:21 2006 From: Virginia.Achstetter <@t> afip.osd.mil (Achstetter, Virginia A.) Date: Fri Oct 6 12:06:59 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 13 In-Reply-To: Message-ID: <127D612DBCD77546A12C4746F7491C7F111CC7@lewis.afip.osd.mil> The grandfather clause, I remember it well, did not GIVE you an HTL, it just allowed you to take the HTL exam without the other requirements of education. So, if you only had a high school education but had been a registered HT for say 10 years, you were allowed to take the exam. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, October 06, 2006 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 35, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: histotechs (Patsy Ruegg) 2. RE: Histotechs: how many are we nationwide? (Weems, Joyce) 3. RE: phalloidin? (Weems, Joyce) 4. RE: Histotechs: how many are we nationwide? (Rene J Buesa) 5. RE: Histotechs: how many are we nationwide? (Dawson, Glen) ---------------------------------------------------------------------- Message: 1 Date: Fri, 6 Oct 2006 10:52:39 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] histotechs To: "'Rene J Buesa'" , "'Dolores Townsend'" , , Message-ID: <006101c6e967$d66b7e30$6601a8c0@Patsy> Content-Type: text/plain; charset="iso-8859-1" You do not have to pay dues to ASCP to stay registered as an HT. The one time exam and certification is for life. Once you are certified by ASCP you can always call yourself an HT. They have initiated a continuing education requirement for those new HT's taking their certification, now you must submit proof of CEU's to maintain your certification. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:00 AM To: Dolores Townsend; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: Re: [Histonet] histotechs Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 6 Oct 2006 12:52:59 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Histotechs: how many are we nationwide? To: "Patsy Ruegg" , "Rene J Buesa" , "LuAnn Anderson" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED44@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" And you all must know that we now should be saying "Grandparented" in - instead of Grandfathered... a bit of Friday humor (very light I know) j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Friday, October 06, 2006 12:49 PM To: 'Rene J Buesa'; 'LuAnn Anderson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Rene, I have never heard of anyone being "grandfathered" in as an HT. Perhaps you are speaking of when the HTL certification first began around 1980? At that time if you were a certified HT with so many years of experience (?10) you could be grandfathered in as an HTL without having the required BS degree, but I believe you still had to take the HTL exam. ASCP certifies HT/HTL's and they do have an eligibility route which may include OJT but everyone has to take the exam to be called an HT. I know there are plenty of people working and doing a good job who do not have the certification from ASCP, so why don't they just try to take the exam and get the certification? Allowing anyone doing histology work to be called an HT would I believe diminish the significance of those of us who have bothered to be recognized as professionally certified by ASCP. Medical technology is a field that is regulated requiring that those in laboratory medicine be certified to do the test for patients that matter so much in their health care. Would you want just anybody performing such tests on you or your loved ones? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:19 AM To: LuAnn Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren?? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren?? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren?? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren?? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren?? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1??/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 3 Date: Fri, 6 Oct 2006 12:53:51 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] phalloidin? To: "Kathy Cormier" , Cc: histonet@pathology.swmed.edu Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED45@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Thank you so much. I just googled and was trying to wade through all that stuff!!! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kathy Cormier Sent: Friday, October 06, 2006 12:49 PM To: jjurczak@att.net Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] phalloidin? I just found out what is was last week, so I know what you are thinking...We just used it to stain filamentous actin in our tissue sections. We had a native population of cells that autofluoresced, and wanted to get "fluorescent H and E" to go along w/ it. It worked quite nicely, but it is really really toxic. Kathy ----- Original Message ----- From: jjurczak@att.net To: Kathy Cormier Sent: Friday, October 06, 2006 12:33 PM Subject: Re: [Histonet] phalloidin? Excuse my ignorance, but what is pahlloidin and what do you do with it? -------------- Original message from "Kathy Cormier" : -------------- > Hello 'Netters, > > Does anyone know of any phalloidin replacement that works as nicely as > phalloidin, but safer to work with? (one never knows right?) We would be using > this on frozen mouse sections... > > Thanks and have a great long weekend! > > Kathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 4 Date: Fri, 6 Oct 2006 09:56:35 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Histotechs: how many are we nationwide? To: "Morken, Tim" , histonet@lists.utsouthwestern.edu Message-ID: <20061006165635.73765.qmail@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Tim: I could not have said it better (even if that is really easy, I mean, saying something better than me!). Now since I get very focused (at times and while I am free from Al's grip; Alzheimer's I mean): I have to understand that without much difficulty we could be more than the 49,000 I "guestimated"? Ren? J. "Morken, Tim" wrote: Rene, The answer to several of your questions has to do with how a histotech fits in the medical field. The salient fact is that the histotech does not make any diagnostic decisions. That is the province of the pathologist. On the other hand, med techs are "signing out" cases all the time in the "clinical" lab and the pathologist only is involved when there is a discrepancy (sometimes not even then). This happens because med techs are for the most part reporting quantitiative values taken from their instruments. In microbiology they are reporting out postive/negative infections. Cytologists are reporting out diagnoses on interpreted information - far beyond most med tech work - and they have legal liability for it. Because of this diagnostic responsibility the med tech and cytotech has a much higher profile in the medical field than histotechs. Histotechs do the work, pathologists take all the glory (such as it is!). The histotech, in the US at least, is almost always an on-the-job trained person from a background that is probably not medical, or even biology related. The historical baggage has led to the field being seen by most as a backwater even today. Of course we on Histonet know that histotechnology has come a long, long way since the days when all we did was h&e's and special stains. I realized how far when pathologists began asking me which antibodies we should run on various cases. I knew more than some of them! Histotechnolgy is the equal of any other medical lab field in terms of technology and relevance, but it is still not a diagnostic job. We provide information that must be interpreted, and unfortunately (for histotechs) in most cases it requires great expertise to interpret. I am actually quite amazed that histotechs can now demand the same pay as med techs in most places, and in some places even more. Indeed, in my home area, the San Francisco Bay Area, a histotech with only a few years of experience can demand 50K to 70K for only basic histology work (not IHC, that pays even more!) - and labs will fight over them. That is far different than 25 years ago. The fact some labs hire unskilled people and train on the job is a fact of life. As noted, there are few schools for it, and only a handful of students will ever even hear about the profession (thus my appearance in a high school textbook). Even if a student did hear about histotechnology, how would they get into it without extensive help? Every single lab I have visited wants more techs but cannot find any. Since they are allowed to use unskilled people, and train them, they do (and pay much less - no qualifications, no degree, no certification, why pay more?). The only way that will not happen is if hundreds of schools are opened. Who will teach at these schools? The only people qualified to do so are working in the field. How many of those want to teach? I've considered it but it is a big job so who knows.... So how may histotechs? A LOT more than the numbers suggest. The vast majority are not certified, do not go to meetings and never hear anything about such things. I'm not sure anyone has every tried to even figure that out (besides Rene!). Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 7:31 AM To: Pamela Marcum; Vinnie Della Speranza; DeBrosse_Beatrice@Allergan.com; histonet-bounces@lists.utsouthwestern.edu; doug@ppspath.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Pamela: The number is probably higher,but we should try to know, strength is in the numbers. Why we are not even considered amongst the medical professionals in the US Census? Why nobody knows how many are needed to cover the retiring HTs, those that are "baby boomers'? Why there is not a cry for more schools? Why it is allowed that some "private" labs pay functioning histotechs salaries below those of other medical lab professionals? Why is has been just now (2005) that the HTs salaries have been more or less reached the other salaries in the ML? The oficial figures of ASCP certified have to be reduced at least by half; I do not envision many HT(ASCP) certified in 1948 to be alive! And the same goes for the HTL, I am one already retired and I am not alone! Ren? J. Pamela Marcum wrote: One area I don't see addressed is we may have had 20,000 registered but how many are still working. I was registered in the eight thousands in the mid seventies so the increase is not that great compared to what we seem to need now and the fact that many of us will retire over the next 5 to 10 years. The number of HTLs may increase that number however we are still woefully short of Histologist in all areas. I think actual numbers are hard to some by with all things considered. Pam Marcum At 05:55 PM 10/5/2006, Vinnie Della Speranza wrote: >I'm trying desperately to stay out of this discussion but I can help >with the number you've requested. the NSH is making plans to celebrate >the 20,000th HT(ASCP) which is expected to occur this Fall. I don't >have #s of HTLs but it is of course much smaller. >Vinnie > > >>> "Douglas D Deltour" 10/05/06 05:18PM >>> >If that is the case then we should be able to get somewhat of an >accurate number from ASCP. I would assume??? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity >to which it is addressed and may contain information that is >privileged, confidential and exempt from disclosure under applicable >law. If the reader of this message is not the intended recipient, you >are hereby notified that any dissemination, distribution, or copying of >this communication is strictly prohibited by law. If you have received >this communication in error, please notify me immediately. > >-----Original Message----- >From: DeBrosse_Beatrice [mailto:DeBrosse_Beatrice@Allergan.com] >Sent: Thursday, October 05, 2006 5:10 PM >To: Douglas D Deltour; LuAnn Anderson; Rene J Buesa; LaDonna G. Elpers; >Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Maybe, but it would make the number a whole lot more accurate if only >the registered technicians would be accounted for. Otherwise, why even >bother to have a registration. > >That doesn't have anything do to with the quality of registered verses >nonregistered histotechs. I know from experience they can do just as >good of a job. But you have to draw the line somewhere. > >Beatrice DeBrosse-Serra >HT(ASCP)QIHC >Allergan, Inc. >2525 Dupont Drive RD-2A >Irvine, CA 92612 >714-246-5116 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >D Deltour >Sent: Thursday, October 05, 2006 1:44 PM >To: 'LuAnn Anderson'; 'Rene J Buesa'; 'LaDonna G. Elpers'; 'Jackie M >O'Connor'; 'Zajic Kari' >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >Can of worms... >If you pass a NAACLS accredited school or get a degree in histology >then why wouldn't you call them a HT? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity >to which it is addressed and may contain information that is >privileged, confidential and exempt from disclosure under applicable >law. If the reader of this message is not the intended recipient, you >are hereby notified that any dissemination, distribution, or copying of >this communication is strictly prohibited by law. If you have received >this communication in error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn >Anderson >Sent: Thursday, October 05, 2006 4:30 PM >To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >I think that certification should be the issue. >One does not truly have the title of HTif they have not passed the >registry exam. JMHO. > >At 02:47 PM 10/5/2006, Rene J Buesa wrote: > >No, certification is not the issue, just how many HTs there are, how > >many of us share this art of histology? > > Ren? J. > > > >"LaDonna G. Elpers" wrote: > > Our facility has 6 Histology Technicians, my guess would be that > >there are probably another > > 15 in our town. The question would be if we talking about those > >certified only? We are currently half and half, 3 certified and 3 > >not. > > > >LaDonna G. Elpers, BA, HT(ASCP) > >Manager, Histology > >BASi (Bioanalytical Systems, Inc.) > >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 > >ext 128 F 812.985.3403 lelpers@bioanalytical.com > >www.bioanalytical.com > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > >On Behalf Of Jackie M O'Connor > >Sent: Thursday, October 05, 2006 1:46 PM > >To: Zajic Kari > >Cc: histonet@lists.utsouthwestern.edu; > >histonet-bounces@lists.utsouthwestern.edu > >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > > > >What about those of us who are doing the work of four people? Does that > >count? > >JO'C > > > > > > > >"Zajic Kari" > >Sent by: histonet-bounces@lists.utsouthwestern.edu > >10/05/2006 01:28 PM > > > >To > >"Rene J Buesa" , > >cc > > > >Subject > >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > > > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very > >interesting! I am only one in my facility, a general hospital. If you are > >taking count!! :) > > > >You have now inspired me to re-new my severly overdue membership with the > >NSH. > >Cheers from West Palm! > >Kari :) > > > >Kari Marie Zajic HTL,MLT > >Histology/Pathology Supervisor > >Palms West Hospital > >Pathology Department > >13001 State Road Eighty > >Loxahatchee, Florida 33470 > >phone: (561)798-6036 > >fax: (561)753-4298 > >voicemail: (561)753-4299 > >pager: (561)610-4949 > >email: Kari.Zajic@HCAHealthcare.com > > > >This email and any files transmitted with it may contain PRIVILEGED or > >CONFIDENTIAL > >information and may be read or used only by the intended recipient. If you > >are not the intended recipient of the email or any of its attachment, > >please be advised that you have received this email in error and that any > >use, dissemination, distribution, forwarding, printing, or copying of this > >email or any attached files is strictly prohibited. If you have received > >this email in error, please immediately purge it and all attachments and > >notify the sender by reply email or contact the sender at the number > >listed. > > > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > >Buesa > >Sent: Thursday, October 05, 2006 1:32 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Histotechs: how many are we nationwide? > > > > > >Dear Histonet subscriber: > > > >The US Census does NOT recognize histotechs (HTs) as a category of > >professionals (the NSH is going to try to do something about that during > >2007) so we do not know how many we are. Some indirect calculations are > >needed to answer that question. > >According to the US Census bureau: > > > >1- there are 5,193 general and surgical hospitals and perhaps 95% of > >them have a histology laboratory = 5,000 labs > >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in > >some). > >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > > > >3- There are 4,084 institutions of higher education and perhaps 50% have > >some sort of histology lab, veterinary, marine, research, botany, etc. = > >2,000 histology labs. > >4- those experimental labs have an average of 2 HTs (between 1 and 5, > >sometimes up to 10) > >So, in universities there could be 2,000 x 2 = 4,000 HTs > > > >Adding both = 49,000 histotechs nationwide. > > > >If membership to the NSH in 2006 (4,400 members) is any indicator (and > >usually prestigious professional societies are able to have a membership > >of roughly 10% of the profession) it can be said that the 4,400 members in > >2006 = 9% of the proposed number. > > > >What do you think? > >Are we more than 49,000; less? or is 49,000 HTs about right? > >Ren? J. > > > > > > > > > >--------------------------------- > >Do you Yahoo!? > >Get on board. You're invited to try the new Yahoo! Mail. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >--------------------------------- > >Talk is cheap. Use Yahoo! Messenger to make > >PC-to-Phone calls. Great rates starting at 1?/min. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. ------------------------------ Message: 5 Date: Fri, 6 Oct 2006 11:58:25 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] Histotechs: how many are we nationwide? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sounds more difficult than trying to take conduct a census since unregistered techs would be like trying to find all the homeless that could be in the woods, under a bridge, etc... Also, lets be wary about putting histotechs into the same bucket as artists since most artist are called "struggling" for a reason & histotechs have bills to pay. Put your hackles down, just some poor Friday humor. Glen Dawson Milwaukee, WI ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 13 **************************************** From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Oct 6 12:31:35 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Oct 6 12:31:46 2006 Subject: [Histonet] RE: phalloidin References: <200610061702.FZU58303@mprelay.uc.edu> Message-ID: Along the lines of phalloidin, I tried something else that I am reporting here did NOT work. Our frozen sections were coming off the slides during immunostaining so I tried something I have always wanted to try. We dipped the frozen sections (after air drying) in gelatin (pure 1 or 4% gelatin, in water, autoclaved to dissolve). Then air dried them vertically so they had a thin film. Then we tried to immunostain for BrdU after that. NOTHING worked!! I haven't tried staining for any other antigens. We used fairly long incubation times, so penetration theoretically should not have been a problem. (Overnight for primary, 2 hours each for biotinylated-secondary and ABC). The sections stayed on better, but no staining. Just FYI. Perhaps others have tried something similar? Does anything work? Sarah Pixley From CBark <@t> memorialcare.org Fri Oct 6 13:06:09 2006 From: CBark <@t> memorialcare.org (Christine Bark) Date: Fri Oct 6 13:06:32 2006 Subject: [Histonet] RE:Leica coverslipper Message-ID: <59FE4C55358C6A42B84BAEC6092CEDBC0DEE33F1@sbnt7.memnet.org> We use Anapath glass coverslips (product code: SL102450)from Statlab. We've found that those work the best for us. Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org Message: 7 Date: Fri, 06 Oct 2006 10:43:16 -0400 From: "Angela Bitting" Subject: [Histonet] Leica's auto coverslipper To: Message-ID: <45263344020000C9000006FE@GHSGWIANW5V.GEISINGER.EDU> Content-Type: text/plain; charset=US-ASCII We're getting 2 new Leica coverslippers this month. Can other users email me and let me know which brands of coverslips they prefer to use with this instrument? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Sign up for your free MemorialCare Medical Information and Access Card at http://www.memorialcare.com/apps/AccessCard/AboutCard.cfm From akbitting <@t> geisinger.edu Fri Oct 6 13:54:02 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Oct 6 13:54:20 2006 Subject: [Histonet] STP420 from Richard Allan Message-ID: <45266E0A020000C900000737@GHSGWIANW5V.GEISINGER.EDU> Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From mtarango <@t> nvcancer.org Fri Oct 6 14:04:06 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Oct 6 14:04:29 2006 Subject: [Histonet] STP420 from Richard Allan Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1C9C3@NVCIEXCH02.NVCI.org> I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From rjbuesa <@t> yahoo.com Fri Oct 6 14:18:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 14:18:11 2006 Subject: [Histonet] histotechs In-Reply-To: <006101c6e967$d66b7e30$6601a8c0@Patsy> Message-ID: <20061006191804.8217.qmail@web61216.mail.yahoo.com> The Certification Management Program has been created for those certified AFTER January 2004, meaning that those certified before have been "grandfathered" from this new requirement! Ren? J. Patsy Ruegg wrote: You do not have to pay dues to ASCP to stay registered as an HT. The one time exam and certification is for life. Once you are certified by ASCP you can always call yourself an HT. They have initiated a continuing education requirement for those new HT's taking their certification, now you must submit proof of CEU's to maintain your certification. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:00 AM To: Dolores Townsend; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: Re: [Histonet] histotechs Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From rjbuesa <@t> yahoo.com Fri Oct 6 14:22:45 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 14:22:55 2006 Subject: [Histonet] Histotechs: how many are we nationwide? In-Reply-To: <007601c6e96c$4ffbf550$6601a8c0@Patsy> Message-ID: <20061006192245.78634.qmail@web61212.mail.yahoo.com> Patsy: It seems that you did not read my original e-mail: a- 95% of 5,193 lbas with histology services x 9 HTs per lab = 45,000 HTs b- 50% of 4,084 universities with histology services x 2 HTs lab= 4,000 HTs Total = 49,000 HTs It is a "guestimate" but with some starting point. So far the majority of those with some opinion about the number, consider it on the "low side" because many other types of labs are not included. Ren? J. Patsy Ruegg wrote: v\:* {behavior:url(#default#VML);} o\:* {behavior:url(#default#VML);} w\:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} st1\:*{behavior:url(#default#ieooui) } You mean 49,000 doing histology work? If so, I do not know and do not know how you would measure that unless you did it by clinical cases done and sort of assign a technician an average # of cases and calculate it that way. That would not include all those in research and vet science, unless you include vet science in your clinical case value. I know that ASCP is about to certify the 20,000th HT they are on 19885 or something like that. The 20Kth HT will be certified this year. NSH is planning to celebrate that somehow. Patsy --------------------------------- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, October 06, 2006 11:01 AM To: Patsy Ruegg Subject: RE: [Histonet] Histotechs: how many are we nationwide? Patsy: I use "HT" as an abbreviation for "histotech" wehich is easier to write. Any State, with licensure requirements, "grandfathered" those working in histology that were required by the State to have a State licensure to work. That is the "grandfathering" I am referring to. Those States that even today do not require their HTs to have a State license did not have to do that. I am not writing about the ASCP HT licensure! But to the question: do you think we are 49,000?; more?; less? Ren? J. Patsy Ruegg wrote: Rene, I have never heard of anyone being "grandfathered" in as an HT. Perhaps you are speaking of when the HTL certification first began around 1980? At that time if you were a certified HT with so many years of experience (?10) you could be grandfathered in as an HTL without having the required BS degree, but I believe you still had to take the HTL exam. ASCP certifies HT/HTL's and they do have an eligibility route which may include OJT but everyone has to take the exam to be called an HT. I know there are plenty of people working and doing a good job who do not have the certification from ASCP, so why don't they just try to take the exam and get the certification? Allowing anyone doing histology work to be called an HT would I believe diminish the significance of those of us who have bothered to be recognized as professionally certified by ASCP. Medical technology is a field that is regulated requiring that those in laboratory medicine be certified to do the test for patients that matter so much in their health care. Would you want just anybody performing such tests on you or your loved ones? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:19 AM To: LuAnn Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From doug <@t> ppspath.com Fri Oct 6 14:31:10 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Oct 6 14:28:08 2006 Subject: [Histonet] STP420 from Richard Allan In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDEB1C9C3@NVCIEXCH02.NVCI.org> Message-ID: Mark, How many blocks are you putting through it a day? Thanks Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 3:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 6 14:31:23 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 14:31:33 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 13 In-Reply-To: <127D612DBCD77546A12C4746F7491C7F111CC7@lewis.afip.osd.mil> Message-ID: <20061006193123.59845.qmail@web61217.mail.yahoo.com> But if for instance you starting working in a histology lab in 1960, were trained on the job, had no theoretical training and "all of the sudden" the State required the histotechs you to have a State license (like in Florida) you were allowed to keep working without having to pass any test, you were "grandfathered" into a license that allowed you to keep working, now with the designation of histotech. Ren? J. "Achstetter, Virginia A." wrote: The grandfather clause, I remember it well, did not GIVE you an HTL, it just allowed you to take the HTL exam without the other requirements of education. So, if you only had a high school education but had been a registered HT for say 10 years, you were allowed to take the exam. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, October 06, 2006 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 35, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: histotechs (Patsy Ruegg) 2. RE: Histotechs: how many are we nationwide? (Weems, Joyce) 3. RE: phalloidin? (Weems, Joyce) 4. RE: Histotechs: how many are we nationwide? (Rene J Buesa) 5. RE: Histotechs: how many are we nationwide? (Dawson, Glen) ---------------------------------------------------------------------- Message: 1 Date: Fri, 6 Oct 2006 10:52:39 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] histotechs To: "'Rene J Buesa'" , "'Dolores Townsend'" , , Message-ID: <006101c6e967$d66b7e30$6601a8c0@Patsy> Content-Type: text/plain; charset="iso-8859-1" You do not have to pay dues to ASCP to stay registered as an HT. The one time exam and certification is for life. Once you are certified by ASCP you can always call yourself an HT. They have initiated a continuing education requirement for those new HT's taking their certification, now you must submit proof of CEU's to maintain your certification. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:00 AM To: Dolores Townsend; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: Re: [Histonet] histotechs Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 6 Oct 2006 12:52:59 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Histotechs: how many are we nationwide? To: "Patsy Ruegg" , "Rene J Buesa" , "LuAnn Anderson" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED44@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" And you all must know that we now should be saying "Grandparented" in - instead of Grandfathered... a bit of Friday humor (very light I know) j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Friday, October 06, 2006 12:49 PM To: 'Rene J Buesa'; 'LuAnn Anderson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Rene, I have never heard of anyone being "grandfathered" in as an HT. Perhaps you are speaking of when the HTL certification first began around 1980? At that time if you were a certified HT with so many years of experience (?10) you could be grandfathered in as an HTL without having the required BS degree, but I believe you still had to take the HTL exam. ASCP certifies HT/HTL's and they do have an eligibility route which may include OJT but everyone has to take the exam to be called an HT. I know there are plenty of people working and doing a good job who do not have the certification from ASCP, so why don't they just try to take the exam and get the certification? Allowing anyone doing histology work to be called an HT would I believe diminish the significance of those of us who have bothered to be recognized as professionally certified by ASCP. Medical technology is a field that is regulated requiring that those in laboratory medicine be certified to do the test for patients that matter so much in their health care. Would you want just anybody performing such tests on you or your loved ones? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:19 AM To: LuAnn Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? Well I think we have been "flamming" since yesterday! Have a nice weekend! Ren?? J. LuAnn Anderson wrote: Don't have a clue. How's this for "flaming Fridays"????? :o) At 10:36 AM 10/6/2006, Rene J Buesa wrote: LuAnn: I write "HT" because it has less letters than writing "histotech", no reference to the "Histotechnician" ASCP title. It is just easier to type! But again: with that in mind: what do you think: are we 49,000?; less?' more? Ren?? J. LuAnn Anderson wrote: I never said nor did I imply that people who are not certified are not capable of doing a good job. I know very well that they can--I have trained many myself. I simply said that if you are asking for the number of "HT's" then you would need to be counting those certified--just because one is doing histology does not give them the title of HT--technically one must pass the registry to have that title. If you are just looking for the total number of people who work in histology labs--then that is a different question all together.~~LuAnn At 07:45 AM 10/6/2006, Rene J Buesa wrote: And who says I don't call them HTs? For me any person doing histology work is an HT; have you forgotten how many "trained just on the job" HTs were grandfathered when a license was a requirement? Is a fantastic section or a beautiful trichrome done better by somebody accredited over somebody with the right experience? (Another "can of worms"). This is just a "number" of HTs nationwide = how many of us are doing the slides on which the pathologists base their diagnosis. Ren?? J. Douglas D Deltour wrote: Can of worms... If you pass a NAACLS accredited school or get a degree in histology then why wouldn't you call them a HT? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, October 05, 2006 4:30 PM To: Rene J Buesa; LaDonna G. Elpers; Jackie M O'Connor; Zajic Kari Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotechs: how many are we nationwide? I think that certification should be the issue. One does not truly have the title of HTif they have not passed the registry exam. JMHO. At 02:47 PM 10/5/2006, Rene J Buesa wrote: >No, certification is not the issue, just how >many HTs there are, how many of us share this art of histology? > Ren?? J. > >"LaDonna G. Elpers" wrote: > Our facility has 6 Histology Technicians, my > guess would be that there are probably another > 15 in our town. The question would be if we > talking about those certified only? We are > currently half and half, 3 certified and 3 not. > >LaDonna G. Elpers, BA, HT(ASCP) >Manager, Histology >BASi (Bioanalytical Systems, Inc.) >10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 >P 812.985.5900 ext 128 >F 812.985.3403 >lelpers@bioanalytical.com > www.bioanalytical.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Jackie M O'Connor >Sent: Thursday, October 05, 2006 1:46 PM >To: Zajic Kari >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] Histotechs: how many are we nationwide? > >What about those of us who are doing the work of four people? Does that >count? >JO'C > > > >"Zajic Kari" >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/05/2006 01:28 PM > >To >"Rene J Buesa" , >cc > >Subject >RE: [Histonet] Histotechs: how many are we nationwide? > > > > > > >Hi Rene, I enjoyed your recent article in the new Advance, was very >interesting! I am only one in my facility, a general hospital. If you are >taking count!! :) > >You have now inspired me to re-new my severly overdue membership with the >NSH. >Cheers from West Palm! >Kari :) > >Kari Marie Zajic HTL,MLT >Histology/Pathology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >fax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that any >use, dissemination, distribution, forwarding, printing, or copying of this >email or any attached files is strictly prohibited. If you have received >this email in error, please immediately purge it and all attachments and >notify the sender by reply email or contact the sender at the number >listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J >Buesa >Sent: Thursday, October 05, 2006 1:32 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histotechs: how many are we nationwide? > > >Dear Histonet subscriber: > >The US Census does NOT recognize histotechs (HTs) as a category of >professionals (the NSH is going to try to do something about that during >2007) so we do not know how many we are. Some indirect calculations are >needed to answer that question. >According to the US Census bureau: > >1- there are 5,193 general and surgical hospitals and perhaps 95% of >them have a histology laboratory = 5,000 labs >2- the average number of HTs per lab = 9 (range from 1 to 15 or more in >some). >So, in hospital settings there could be 5,000 x 9 = 45,000 HTs > >3- There are 4,084 institutions of higher education and perhaps 50% have >some sort of histology lab, veterinary, marine, research, botany, etc. = >2,000 histology labs. >4- those experimental labs have an average of 2 HTs (between 1 and 5, >sometimes up to 10) >So, in universities there could be 2,000 x 2 = 4,000 HTs > >Adding both = 49,000 histotechs nationwide. > >If membership to the NSH in 2006 (4,400 members) is any indicator (and >usually prestigious professional societies are able to have a membership >of roughly 10% of the profession) it can be said that the 4,400 members in >2006 = 9% of the proposed number. > >What do you think? >Are we more than 49,000; less? or is 49,000 HTs about right? >Ren?? J. > > > > >--------------------------------- >Do you Yahoo!? >Get on board. You're invited to try the new Yahoo! Mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at 1??/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. Stay in the know. Pulse on the new Yahoo.com. Check it out. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 3 Date: Fri, 6 Oct 2006 12:53:51 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] phalloidin? To: "Kathy Cormier" , Cc: histonet@pathology.swmed.edu Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED45@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Thank you so much. I just googled and was trying to wade through all that stuff!!! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kathy Cormier Sent: Friday, October 06, 2006 12:49 PM To: jjurczak@att.net Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] phalloidin? I just found out what is was last week, so I know what you are thinking...We just used it to stain filamentous actin in our tissue sections. We had a native population of cells that autofluoresced, and wanted to get "fluorescent H and E" to go along w/ it. It worked quite nicely, but it is really really toxic. Kathy ----- Original Message ----- From: jjurczak@att.net To: Kathy Cormier Sent: Friday, October 06, 2006 12:33 PM Subject: Re: [Histonet] phalloidin? Excuse my ignorance, but what is pahlloidin and what do you do with it? -------------- Original message from "Kathy Cormier" : -------------- > Hello 'Netters, > > Does anyone know of any phalloidin replacement that works as nicely as > phalloidin, but safer to work with? (one never knows right?) We would be using > this on frozen mouse sections... > > Thanks and have a great long weekend! > > Kathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 4 Date: Fri, 6 Oct 2006 09:56:35 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Histotechs: how many are we nationwide? To: "Morken, Tim" , histonet@lists.utsouthwestern.edu Message-ID: <20061006165635.73765.qmail@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Tim: I could not have said it better (even if that is really easy, I mean, saying something better than me!). Now since I get very focused (at times and while I am free from Al's grip; Alzheimer's I mean): I have to understand that without much difficulty we could be more than the 49,000 I "guestimated"? === message truncated === --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From pruegg <@t> ihctech.net Fri Oct 6 14:53:48 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Oct 6 14:53:59 2006 Subject: [Histonet] histotechs In-Reply-To: <20061006191804.8217.qmail@web61216.mail.yahoo.com> Message-ID: <00ab01c6e981$24dbb7b0$6601a8c0@Patsy> True, but even those of us not required to show CMP compliance are encouraged to take part and present our evidence of continuing education in our field. I think this is a great opportunity for NSH and others to offer more education for those who now will be required to have it to maintain their certification. I see this as a very positive move in the right direction to promote HT?s as professionals. Patsy _____ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, October 06, 2006 1:18 PM To: Patsy Ruegg; 'Dolores Townsend'; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: RE: [Histonet] histotechs The Certification Management Program has been created for those certified AFTER January 2004, meaning that those certified before have been "grandfathered" from this new requirement! Ren? J. Patsy Ruegg wrote: You do not have to pay dues to ASCP to stay registered as an HT. The one time exam and certification is for life. Once you are certified by ASCP you can always call yourself an HT. They have initiated a continuing education requirement for those new HT's taking their certification, now you must submit proof of CEU's to maintain your certification. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:00 AM To: Dolores Townsend; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: Re: [Histonet] histotechs Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From doug <@t> ppspath.com Fri Oct 6 14:59:54 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Oct 6 14:56:53 2006 Subject: [Histonet] STP420 from Richard Allan In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDEB1C9C5@NVCIEXCH02.NVCI.org> Message-ID: Thanks for that bit of info Mark. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 -----Original Message----- From: Tarango, Mark [mailto:mtarango@nvcancer.org] Sent: Friday, October 06, 2006 3:46 PM To: Douglas D Deltour Subject: RE: [Histonet] STP420 from Richard Allan I don't even use it some days. When I do, I put only a few blocks on it (like 10). I've used both chambers at the same time though, and it works well. The rotation does seem to speed up the processing quite a bit. It can same some processing time, if that's what you're interested in. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Douglas D Deltour [mailto:doug@ppspath.com] Sent: Friday, October 06, 2006 12:31 PM To: Tarango, Mark; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan Mark, How many blocks are you putting through it a day? Thanks Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 3:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ---- -- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================ == From rjbuesa <@t> yahoo.com Fri Oct 6 15:02:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 6 15:02:35 2006 Subject: [Histonet] histotechs In-Reply-To: <00ab01c6e981$24dbb7b0$6601a8c0@Patsy> Message-ID: <20061006200228.90113.qmail@web61212.mail.yahoo.com> I totally agree! I just would like to know how many will do the continued education requirements without having to. The case in Florida is different: we have to have continued education certification biannually to keep our license updated; I do not know about other States and their regulations. Ren? J. Patsy Ruegg wrote: v\:* {behavior:url(#default#VML);} o\:* {behavior:url(#default#VML);} w\:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} True, but even those of us not required to show CMP compliance are encouraged to take part and present our evidence of continuing education in our field. I think this is a great opportunity for NSH and others to offer more education for those who now will be required to have it to maintain their certification. I see this as a very positive move in the right direction to promote HT?s as professionals. Patsy --------------------------------- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, October 06, 2006 1:18 PM To: Patsy Ruegg; 'Dolores Townsend'; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: RE: [Histonet] histotechs The Certification Management Program has been created for those certified AFTER January 2004, meaning that those certified before have been "grandfathered" from this new requirement! Ren? J. Patsy Ruegg wrote: You do not have to pay dues to ASCP to stay registered as an HT. The one time exam and certification is for life. Once you are certified by ASCP you can always call yourself an HT. They have initiated a continuing education requirement for those new HT's taking their certification, now you must submit proof of CEU's to maintain your certification. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 06, 2006 10:00 AM To: Dolores Townsend; Histonet@lists.utsouthwestern.edu; failm@musc.edu Subject: Re: [Histonet] histotechs Dolores: Not only how many are paying their dues, but how many are already DEAD or RETIRED? Remember that those figures go back to certifications from 1948 (HT) and 1980 (HTL). I for one am retired (one HTL less from the count!). Ren? J. Dolores Townsend wrote: The number given for ASCP registered HT and HTL seems awfully low to me. So, another thing to consider: of those ASCP registered, how many are actually paying their dues to the ASCP and counted in the total? If only half of all HTs and HTLs are counted, that brings the numbers even higher. Going back to experience vs. certification, I worked for a small hospital and was HT, ASCP but some snotty MT once told me that we were not "real" techs. What is that suppose to mean? I'd like to see her do my job any day and see how she would get along... Dolores --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From llewllew <@t> shaw.ca Fri Oct 6 16:03:07 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Oct 6 16:03:31 2006 Subject: [Histonet] Histotechs: how many are we nationwide? References: <20061006165635.73765.qmail@web61214.mail.yahoo.com> Message-ID: <003f01c6e98a$d2bfc660$130e4246@yourlk4rlmsu> It was said in a post from someone: "The answer to several of your questions has to do with how a histotech fits in the medical field. The salient fact is that the histotech does not make any diagnostic decisions. That is the province of the pathologist. On the other hand, med techs are "signing out" cases all the time in the "clinical" lab and the pathologist only is involved when there is a discrepancy (sometimes not even then). This happens because med techs are for the most part reporting quantitiative values taken from their instruments. In microbiology they are reporting out postive/negative infections. Cytologists are reporting out diagnoses on interpreted information - far beyond most med tech work - and they have legal liability for it. " I, like most non US histotechs, have stayed out of this discussion about numbers and certification of HT and HTL, but the comment above caught my eye because we had a very similar discussion in Canada on the subject about 25 years ago, as to whether Histotechnologists do work as responsible as Medical laboratory Technologists. In Canada the debate was started in an attempt by a few who wanted to remove histotechnology from the Medical Laboratory Technologist syllabus. The US is an anomoly in that in most other countries Histotechnology is one of the fundamental disciplines in which Medical Laboratory technologists are trained. In the US it is replaced with mycology, I believe. We were successful in stopping the removal of the subject, but one of the major arguments used was that we were not MLTs because we did not sign out reports. The definition of a MLT is "one who does testing of biological samples", not "a person who signs reports". I always pointed out that a person who cuts frozen sections of a woman's breast while she is being operated on and who may lose the breast completely as a consequence of that section is doing something just a tad more responsible than signing out a urinalysis report saying, "No pus cells seen." Reductio ad absurdio in both cases. As an observation, I understood the original question to be how many people in the US made their living by doing technical work in a histology laboratory rather than how many were formally qualified to do it. Of course, I have always been in favoour of qualifications, preferably at an advanced level. Please excuse my intrusion in a US national debate. From JWEEMS <@t> sjha.org Fri Oct 6 16:07:42 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 6 16:08:01 2006 Subject: [Histonet] Histotechs: how many are we nationwide? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED54@sjhaexc02.sjha.org> Very well said Bryan. You can jump into our conversations any time! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bryan Llewellyn Sent: Friday, October 06, 2006 5:03 PM To: Histonet Subject: Re: [Histonet] Histotechs: how many are we nationwide? It was said in a post from someone: "The answer to several of your questions has to do with how a histotech fits in the medical field. The salient fact is that the histotech does not make any diagnostic decisions. That is the province of the pathologist. On the other hand, med techs are "signing out" cases all the time in the "clinical" lab and the pathologist only is involved when there is a discrepancy (sometimes not even then). This happens because med techs are for the most part reporting quantitiative values taken from their instruments. In microbiology they are reporting out postive/negative infections. Cytologists are reporting out diagnoses on interpreted information - far beyond most med tech work - and they have legal liability for it. " I, like most non US histotechs, have stayed out of this discussion about numbers and certification of HT and HTL, but the comment above caught my eye because we had a very similar discussion in Canada on the subject about 25 years ago, as to whether Histotechnologists do work as responsible as Medical laboratory Technologists. In Canada the debate was started in an attempt by a few who wanted to remove histotechnology from the Medical Laboratory Technologist syllabus. The US is an anomoly in that in most other countries Histotechnology is one of the fundamental disciplines in which Medical Laboratory technologists are trained. In the US it is replaced with mycology, I believe. We were successful in stopping the removal of the subject, but one of the major arguments used was that we were not MLTs because we did not sign out reports. The definition of a MLT is "one who does testing of biological samples", not "a person who signs reports". I always pointed out that a person who cuts frozen sections of a woman's breast while she is being operated on and who may lose the breast completely as a consequence of that section is doing something just a tad more responsible than signing out a urinalysis report saying, "No pus cells seen." Reductio ad absurdio in both cases. As an observation, I understood the original question to be how many people in the US made their living by doing technical work in a histology laboratory rather than how many were formally qualified to do it. Of course, I have always been in favoour of qualifications, preferably at an advanced level. Please excuse my intrusion in a US national debate. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bhewlett <@t> cogeco.ca Fri Oct 6 16:19:53 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Oct 6 16:20:01 2006 Subject: [Histonet] Histotechs: how many are we nationwide? References: <20061006165635.73765.qmail@web61214.mail.yahoo.com> <003f01c6e98a$d2bfc660$130e4246@yourlk4rlmsu> Message-ID: <002501c6e98d$2adaca00$6500a8c0@mainbox> Bryan, Here, here! Bryan ----- Original Message ----- From: "Bryan Llewellyn" To: "Histonet" Sent: Friday, October 06, 2006 5:03 PM Subject: Re: [Histonet] Histotechs: how many are we nationwide? > It was said in a post from someone: > > "The answer to several of your questions has to do with how a histotech > fits in the medical field. The salient fact is that the histotech does not > make any diagnostic decisions. That is the province of the pathologist. On > the other hand, med techs are "signing out" cases all the time in the > "clinical" lab and the pathologist only is involved when there is a > discrepancy (sometimes not even then). This happens because med techs are > for the most part reporting quantitiative values taken from their > instruments. In microbiology they are reporting out postive/negative > infections. Cytologists are reporting out diagnoses on interpreted > information - far beyond most med tech work - and they have legal > liability for it. " > > > I, like most non US histotechs, have stayed out of this discussion about > numbers and certification of HT and HTL, but the comment above caught my > eye because we had a very similar discussion in Canada on the subject > about 25 years ago, as to whether Histotechnologists do work as > responsible as Medical laboratory Technologists. > > In Canada the debate was started in an attempt by a few who wanted to > remove histotechnology from the Medical Laboratory Technologist syllabus. > The US is an anomoly in that in most other countries Histotechnology is > one of the fundamental disciplines in which Medical Laboratory > technologists are trained. In the US it is replaced with mycology, I > believe. We were successful in stopping the removal of the subject, but > one of the major arguments used was that we were not MLTs because we did > not sign out reports. > > The definition of a MLT is "one who does testing of biological samples", > not "a person who signs reports". I always pointed out that a person who > cuts frozen sections of a woman's breast while she is being operated on > and who may lose the breast completely as a consequence of that section is > doing something just a tad more responsible than signing out a urinalysis > report saying, "No pus cells seen." Reductio ad absurdio in both cases. > > As an observation, I understood the original question to be how many > people in the US made their living by doing technical work in a histology > laboratory rather than how many were formally qualified to do it. Of > course, I have always been in favoour of qualifications, preferably at an > advanced level. > > Please excuse my intrusion in a US national debate. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cbass <@t> bidmc.harvard.edu Fri Oct 6 22:50:58 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Fri Oct 6 22:49:18 2006 Subject: [Histonet] how to count nodules in a xenograft model Message-ID: <7E809C0E-69E1-4684-9607-B58080AFE24F@bidmc.harvard.edu> Hello Everyone, I am posting this on behalf of a friend. I know very little about xenograft models and I thought someone on this list may have more experience. Essentially, she is injecting MDA-MB-231 cells i.v. in mouse and studying metastasis. She wants to get a count of the nodules that form in the lung. Does anyone have a good protocol for doing this. She has little histology skills, so most likely she will send the tissue to a lab to process. What is the safest way to collect the tissue, how should it be processed, and how does one get a good idea of the number of nodules formed? Any help would be appreciated. Thanks, Caroline From arvidsonkristen <@t> yahoo.com Sat Oct 7 10:09:15 2006 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sat Oct 7 10:09:21 2006 Subject: [Histonet] fried specimens Message-ID: <20061007150915.43582.qmail@web61320.mail.yahoo.com> Help!! We have had problems with our specimens (all derm) on and off for several years. They get hard and fried looking. The problem is so sporadic. The weird thing is only one piece of the biopsy will be affected(ie. one half of a bisected punch looks fried the other normal). We use some sponges when necessary for smaller pieces (I've heard they can cause problems). We are on a 12 hour processing schedule (which we could probably reduce). We have older VIP processors. We are running 350-550 blocks per day so our processors get pretty full. We have recently lower our paraffin temps and we try to keep spaces between our cassettes as much as possible. We check all reagent levels to make sure they are full. So my questions is 'what else are we missing?' Also, can anyone help me with ideas on shorter processing schedules for skin? Thanks for any input!! Kristen --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Sat Oct 7 10:57:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 7 10:58:00 2006 Subject: [Histonet] fried specimens In-Reply-To: <20061007150915.43582.qmail@web61320.mail.yahoo.com> Message-ID: <20061007155756.75378.qmail@web61215.mail.yahoo.com> Kristen: As usual the problem you mention has several solutions, meaning several causes. Let me see if can be of some assistance: 1- I have no explanation as to why one half looks OK and the other not, but it could be something with the sample itself and if it was just partially immersed in the fixative; one part would be with the fixative and the other dehydrating in the air; 2-a 12 hours protocol for small biopsies is too much; I understand that the HT has to rest and that finishing to load the VIPs at 6PM would be advantageous to have a 12 hours protocol to get the finished cassettes at 6AM, but the dehydration steps could be too long. I think it would be advantageous to dehydrate in graded steps (starting at 70% EthOL and go up to the absolute) but that all those steps take less time than they have now. I think it would preferable to have the processed tissue sitting in melted paraffin for more time than now, than having them too long in the dehydratants. 3- you could also use the tissue processors on delay in a way that the specimens sit in the first formalin station completing the fixation before starting a shorter protocol that will end also at 6AM with less time in each station and without having to sit in paraffin until you start embedding; 4- you also could change to a less "violent" dehydrating/infiltrating medium, like mineral oil. When I changed to MO the sporadic problems we had desappeared totally (I could send you the protocol if you want). 5- I also realize that using sponges is faster, but you could get the problems you are having. I used to try 2 other approaches: wrap the biopsies in tissue paper (somewhat laborious) or use pieces of filter paper, precut to cassette size and used as if they were the sponges. Both gave me good results. 6- never overfill your tissue processors and since you have up to 550 blocks / day it is evident that you have more than 1 tissue processor. Dedicate 1 for a shorter protocol for small specimens (the smaller if they are fewer small specimens of the larger if it is the contrary). To me your problems either start at the sampling step (and you could do not much about it) or while processing (and in that aspect you are the "master of your destiny"!) Hope this will help you! Ren? J. kristen arvidson wrote: Help!! We have had problems with our specimens (all derm) on and off for several years. They get hard and fried looking. The problem is so sporadic. The weird thing is only one piece of the biopsy will be affected(ie. one half of a bisected punch looks fried the other normal). We use some sponges when necessary for smaller pieces (I've heard they can cause problems). We are on a 12 hour processing schedule (which we could probably reduce). We have older VIP processors. We are running 350-550 blocks per day so our processors get pretty full. We have recently lower our paraffin temps and we try to keep spaces between our cassettes as much as possible. We check all reagent levels to make sure they are full. So my questions is 'what else are we missing?' Also, can anyone help me with ideas on shorter processing schedules for skin? Thanks for any input!! Kristen --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From liz <@t> premierlab.com Sat Oct 7 11:56:52 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Oct 7 11:54:36 2006 Subject: [Histonet] how to count nodules in a xenograft model In-Reply-To: <7E809C0E-69E1-4684-9607-B58080AFE24F@bidmc.harvard.edu> Message-ID: <000001c6ea31$969852e0$0300a8c0@domain.Premier> Caroline I have done this in the past but with a different cell line B16F10Mel it's a common animal model of metastatic melanoma. The route is the same iv injection and then counting the number of lesions in the lung. The first thing is at necropsy is to inflate the lungs with fixative, if she has not done this before I believe there are other posts addressing this on the Histonet or even possibly the web, but if she needs some info on that just have her personally e-mail me and I'll go over the protocol. From there we did two things we counted the number of tumors on the outside of the lung first, this can be easy or hard depending upon the size of the tumors, sometimes there are too many to count. From there we would process the lung for routine H&E. This can be done two ways; 1. process the entire lung whole and embed as flat as possible or 2. Remove each of the lobes and then process (if you choose to separate the lobes in processing it might not be a bad idea to count the tumors on the outside of the lung for each lobe, that way you could see if your necropsy data correlates with the histology data). I find when the lobes are separated it's a bit easier to count the number of tumors. There should be some publications on this, for the B16F10Mel cell line, I would search that on the web. I would initially start by just cutting one section and staining with H&E and counting. If she is comparing treatment groups and there are significant differences between the treatments she should be able to see differences by counting just one level. If she is starting to see a trend and maybe the possibility that there are differences in the treatment groups she could then make the decision to cut additional levels and count those also. I've only use the MDA-MB-231 cell line as a sq xenograft before so I'm not certain how visible the tumors would be in the lung sections. It is a breast cancer cell line so some immunostain might be helpful possibly keratin? But why do the immunostain if you can see the tumors in the H&E. If she has any questions she can contact me directly. Hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Friday, October 06, 2006 9:51 PM To: HISTONET Subject: [Histonet] how to count nodules in a xenograft model Hello Everyone, I am posting this on behalf of a friend. I know very little about xenograft models and I thought someone on this list may have more experience. Essentially, she is injecting MDA-MB-231 cells i.v. in mouse and studying metastasis. She wants to get a count of the nodules that form in the lung. Does anyone have a good protocol for doing this. She has little histology skills, so most likely she will send the tissue to a lab to process. What is the safest way to collect the tissue, how should it be processed, and how does one get a good idea of the number of nodules formed? Any help would be appreciated. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1794 (20061006) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From sohail_e <@t> yahoo.com Sat Oct 7 14:01:57 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Sat Oct 7 14:02:02 2006 Subject: [Histonet] How much Triton X 100 is OK for permiability during staining Message-ID: <20061007190157.78792.qmail@web39511.mail.mud.yahoo.com> Hello everyone I would like to know which concentration of Triton X 100 is Ok for permiability during whole mount staining. Thanks Sohail --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From rjbuesa <@t> yahoo.com Sat Oct 7 14:24:48 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 7 14:24:53 2006 Subject: [Histonet] How much Triton X 100 is OK for permiability during staining In-Reply-To: <20061007190157.78792.qmail@web39511.mail.mud.yahoo.com> Message-ID: <20061007192448.32975.qmail@web61213.mail.yahoo.com> Sohail: Triton, as well as Tween 20, can (and should) be used in any (and all) staining solutions to facilitate staining at a 0.05% concentration. In many labs in Russia is used as "standard" procedure. Ren? J. sohail ejaz wrote: Hello everyone I would like to know which concentration of Triton X 100 is Ok for permiability during whole mount staining. Thanks Sohail --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From gu.lang <@t> gmx.at Sun Oct 8 04:33:21 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Oct 8 04:33:15 2006 Subject: [Histonet] general mouse-ihc-stain Message-ID: <000d01c6eabc$cbf99300$c812a8c0@dielangs.at> Hi, perhaps not a really clever question, but I hope somebody can easily explain it to me. Why does a (monoclonal) anti-mouse antibody stain all mouse-tissue, when it is created against the fc-part of mouse-antibodies? Or does this only happen with polyclonal antibodies, that are nt really purified? Is such a secondary anti-mouse ab specific against mouse-tissue in general? Is this just an artefact, that could be used for identifing mouse-cells in other species? Hmmm. Gudrun From Pathrm35 <@t> adelphia.net Sun Oct 8 14:29:17 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Sun Oct 8 14:29:17 2006 Subject: [Histonet] position available in southeast Florida Message-ID: <000001c6eb10$0c007b00$2ea2ac45@D6WRV2B1> We currently are seeking a fulltime, M-F, dayshift histotech for our Delray Beach, Florida dermpath lab. Position requires ASCP certification and a State of Florida histology license. Please contact Dr. Steven Glanz, Medical Director at DrSGlanz@leavittmgt.com for more details and resume submission. Ron Martin From anh2006 <@t> med.cornell.edu Mon Oct 9 01:53:40 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Oct 9 01:53:52 2006 Subject: [Histonet] general mouse-ihc-stain In-Reply-To: <000d01c6eabc$cbf99300$c812a8c0@dielangs.at> References: <000d01c6eabc$cbf99300$c812a8c0@dielangs.at> Message-ID: Hi Gudrun, Anti-mouse IgG stains all IgG in mouse tissue which is located in the blood which fully perfuses organs and tissues from the mouse. The IgG may also be within the extracellular matrix outside the vasculature. It does not stain ALL cells at all but rather specifically stains the IgG in the blood and that which has diffused into the tissues. There is something on the order of 10mg IgG per ml blood so you can imagine why it gives an intense staining pattern ... that is 1000X the amount of primary we normally add for staining!!!! To address your final question more specifically, anti-mouse IgG is NOT a marker of mouse cells in general as if you stain mouse cells in vitro (therefore without the blood perfusion) you will get NO STAINING. -- Andrea At 11:33 AM +0200 10/8/06, Gudrun Lang wrote: >Hi, > >perhaps not a really clever question, but I hope somebody can easily explain >it to me. Why does a (monoclonal) anti-mouse antibody stain all >mouse-tissue, when it is created against the fc-part of mouse-antibodies? Or >does this only happen with polyclonal antibodies, that are nt really >purified? Is such a secondary anti-mouse ab specific against mouse-tissue in >general? Is this just an artefact, that could be used for identifing >mouse-cells in other species? Hmmm. > > > >Gudrun > > >_____________________ -- From mgdelaware <@t> comcast.net Mon Oct 9 07:38:36 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Mon Oct 9 07:38:46 2006 Subject: [Histonet] Histology position Message-ID: <002d01c6eb9f$d6d7eb80$0711c847@D7XQNX91> We have a full-time/part-time Histology position opening, please contact: Marian Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Dr. Dover, DE 19904 (302) 677-0000 From mlm11 <@t> cornell.edu Mon Oct 9 08:01:44 2006 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Mon Oct 9 08:01:12 2006 Subject: [Histonet] free slide holders Message-ID: <6.2.1.2.2.20061009085616.01e847c8@postoffice9.mail.cornell.edu> Hi, Still cleaning out. Small to medium size box of slide holders: cardboard-1 & 2 slide, plastic-1 & 2 slide, both flat. Vertical 2 & 5 slide holders . First address to me gets it. Thanks, Mary Lou Norman From PIXLEYSK <@t> UCMAIL.UC.EDU Mon Oct 9 08:53:46 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Mon Oct 9 08:53:49 2006 Subject: [Histonet] Triton and Secondary Mouse Message-ID: Dear Sohail on Triton: For whole mounts we have used 1% Triton (in PBS or PB with sodium azide) in primary, secondary and ABC (no sodium azide in ABC!) reagents. We incubated with primary antibodies for up to one week, and secondary and ABC each for overnight, with longer and more washes than normal between each step. We use 0.2% for all other IHC. (We are not a histotechnology lab, though, so I don't know if this meets those standards). Dear Gudrun on mouse IHC: When we use a mouse primary antibody on mouse tissues, it is luck if the primary will work properly or not. For a secondary, we use a biotinylated secondary mouse antibody from Jackson that is absorbed against rat tissues. This gives us a very clean background, cleaner than the regular anti-mouse antibody. Theoretically, the secondary should only be attaching to cells of the blood system, like macrophages and tissue lymphocytes. But there is other background. My suggestion is to try the "rat-absorbed" version of anti-mouse. Best wishes Sarah Pixley From specialstainsqueen <@t> hotmail.com Mon Oct 9 09:22:41 2006 From: specialstainsqueen <@t> hotmail.com (Sherri Anderson) Date: Mon Oct 9 09:22:51 2006 Subject: [Histonet] IHC control blocks Message-ID: Good morning everyone! Does anyone know of a company that sells control blocks for IHC? I'm looking for blocks rather than slides because 1) I can cut my own slides and 2) in the long run, the blocks would probably end up being more cost efficient. Does anyone know of such a company? I did a quick search on the web but didn't come up with anything. I'm just looking for controls for some of the more "routine" antibodies: actin, CEA, cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. Thanks for any feedback! Sherri _________________________________________________________________ Try the new Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLMTAG From tcl133 <@t> psu.edu Mon Oct 9 09:30:03 2006 From: tcl133 <@t> psu.edu (Dena Lang) Date: Mon Oct 9 09:30:28 2006 Subject: [Histonet] fluorochrome labels for dynamic bone histology Message-ID: <000401c6ebaf$6c779be0$212b7680@kinesresearch.psu.edu> Hi, I would like to find out the exact type (if possible catalogue #) for: Calcein, Alizarin, and Demeclocycline flourochrome labels that are used for bone dynamic histology. For instance, Sigma offers different types of these chemicals with very different pricing. Any help or advice would be greatly appreciated. Thanks! Dean H. Lang, PhD Biomechanics Laboratory Department of Kinesiology Pennsylvania State University 29 Recreation Building tel. (814)-863-9243 fax (814)-863-4755 The Center for Developmental and Health Genetics tel. (814)-865-1717 EMAIL: tcl133@psu.edu From pruegg <@t> ihctech.net Mon Oct 9 09:38:09 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 9 09:39:36 2006 Subject: [Histonet] IHC control blocks In-Reply-To: Message-ID: <008601c6ebb0$8b6dfb10$6601a8c0@Patsy> Sherri and all, The NSH IHC Resource Group maintains a control tissue bank for use by members of our group. To join the IHCRG go online to www.ihcrg.org there is no charge to join our group of over 400 working in IHC from all over the world, but as this is a committee through NSH, membership with NSH is required and will be confirmed. Best regards, Patsy Ruegg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherri Anderson Sent: Monday, October 09, 2006 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC control blocks Good morning everyone! Does anyone know of a company that sells control blocks for IHC? I'm looking for blocks rather than slides because 1) I can cut my own slides and 2) in the long run, the blocks would probably end up being more cost efficient. Does anyone know of such a company? I did a quick search on the web but didn't come up with anything. I'm just looking for controls for some of the more "routine" antibodies: actin, CEA, cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. Thanks for any feedback! Sherri _________________________________________________________________ Try the new Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLM TAG _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Oct 9 10:24:30 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 9 10:25:09 2006 Subject: [Histonet] IHC control blocks In-Reply-To: References: Message-ID: <452A316F02000077000024E6@hcnwgwds01.hh.chs> Hi Sherri: Do you work in a hospital laboratory? If so, you really should prepare your own control blocks from tissue that you have on site. I am not a proponent of using someone else's tissue (that has been fixed and processed differently) for control purposes. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sherri Anderson" 10/09/06 10:22 AM >>> Good morning everyone! Does anyone know of a company that sells control blocks for IHC? I'm looking for blocks rather than slides because 1) I can cut my own slides and 2) in the long run, the blocks would probably end up being more cost efficient. Does anyone know of such a company? I did a quick search on the web but didn't come up with anything. I'm just looking for controls for some of the more "routine" antibodies: actin, CEA, cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. Thanks for any feedback! Sherri _________________________________________________________________ Try the new Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLMTAG _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Oct 9 11:06:21 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 9 11:06:50 2006 Subject: [Histonet] IHC control blocks In-Reply-To: <452A316F02000077000024E6@hcnwgwds01.hh.chs> Message-ID: <00c001c6ebbc$ddd683c0$6601a8c0@Patsy> Good point Richard. If you don't have the control tissue in house perhaps you should not be doing the test????/ Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, October 09, 2006 9:25 AM To: Sherri Anderson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC control blocks Hi Sherri: Do you work in a hospital laboratory? If so, you really should prepare your own control blocks from tissue that you have on site. I am not a proponent of using someone else's tissue (that has been fixed and processed differently) for control purposes. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sherri Anderson" 10/09/06 10:22 AM >>> Good morning everyone! Does anyone know of a company that sells control blocks for IHC? I'm looking for blocks rather than slides because 1) I can cut my own slides and 2) in the long run, the blocks would probably end up being more cost efficient. Does anyone know of such a company? I did a quick search on the web but didn't come up with anything. I'm just looking for controls for some of the more "routine" antibodies: actin, CEA, cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. Thanks for any feedback! Sherri _________________________________________________________________ Try the new Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLM TAG _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cnunes <@t> ipimar.pt Mon Oct 9 12:12:11 2006 From: cnunes <@t> ipimar.pt (Cristina Nunes) Date: Mon Oct 9 12:12:45 2006 Subject: [Histonet] Periodic acid vs environment Message-ID: <002201c6ebc6$0f686670$a5060a0a@ipimar.pt> Dear all, I have been trying for the first time the PAS staining and have a "practical" problem. How harmful is the periodic acid for the environment? Is it something you may throw out without any harm or do I need to store it for a specialised discard? I hope it is not a very silly question and many thanks for your help. Best regards, Cristina Nunes. From nancy.troiano <@t> yale.edu Mon Oct 9 12:31:00 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Mon Oct 9 12:44:29 2006 Subject: [Histonet] fluorochrome labels for bone Message-ID: <5.2.1.1.2.20061009132647.00c4a118@email.med.yale.edu> We use calcein from EM Sciences, catalog #CX0079-1 (1 gram size) and dose mice at 30 mg/kg IP. We use alizarin red complexone from Sigma, Cat. #A3882. We don't use demeclocycline but use xylenol orange from Sigma, cat #X-0127. From godsgalnow <@t> aol.com Mon Oct 9 12:44:42 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Oct 9 12:45:18 2006 Subject: [Histonet] fried specimens In-Reply-To: <20061007150915.43582.qmail@web61320.mail.yahoo.com> References: <20061007150915.43582.qmail@web61320.mail.yahoo.com> Message-ID: <8C8B9E1BC2048A4-90C-1717@FWM-R16.sysops.aol.com> We had the same problem and we had to rule out one variable at at time....... first, do you have heat on any of your xylenes? If so, remove it. second, what is the heat set at on the wax infiltration on your processor? 60 is too hot for some waxes third, the sponges gotta go.....go to histoscreen cassettes. I know that the cost is more but its worht it in the end. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RighPath Tampa -----Original Message----- From: arvidsonkristen@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Sat, 7 Oct 2006 11:09 AM Subject: [Histonet] fried specimens Help!! We have had problems with our specimens (all derm) on and off for several years. They get hard and fried looking. The problem is so sporadic. The weird thing is only one piece of the biopsy will be affected(ie. one half of a bisected punch looks fried the other normal). We use some sponges when necessary for smaller pieces (I've heard they can cause problems). We are on a 12 hour processing schedule (which we could probably reduce). We have older VIP processors. We are running 350-550 blocks per day so our processors get pretty full. We have recently lower our paraffin temps and we try to keep spaces between our cassettes as much as possible. We check all reagent levels to make sure they are full. So my questions is 'what else are we missing?' Also, can anyone help me with ideas on shorter processing schedules for skin? Thanks for any input!! Kristen --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From mtarango <@t> nvcancer.org Mon Oct 9 12:56:42 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Oct 9 12:56:58 2006 Subject: [Histonet] STP420 from Richard Allan. . Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1CA61@NVCIEXCH02.NVCI.org> Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From AFeatherstone <@t> KaleidaHealth.Org Mon Oct 9 13:02:29 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Oct 9 13:02:35 2006 Subject: [Histonet] IEC MInotome PLUS Cryostat Part Message-ID: <9B4A77DF11463E4FB723D484214AE9BCA55525@KALEXMB02.KaleidaHealth.org> Would anyone have an IEC Minotome Plus Cryostat available for parts? We need the "retract/advance" switch. Annette Featherstone HT/MLT ASCP, MT (HEW) Supervisor Anatomic Pathology Kaleida Health, Buffalo General Hospital 100 High Street Buffalo NY 14203 716-859-2625 FAX: 716-859-1853 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From specialstainsqueen <@t> hotmail.com Mon Oct 9 13:41:23 2006 From: specialstainsqueen <@t> hotmail.com (Sherri Anderson) Date: Mon Oct 9 13:41:35 2006 Subject: [Histonet] IHC control blocks Message-ID: Thanks to everyone for their input. I appreciate the information that was provided. To answer Richard's question, I do not work in a clinical hospital laboratory; therefore, I do not have access to a variety of human control tissues. I simply want to validate my own antibody preparations for a project I am researching. I totally understand what you (and others) are saying about preparing one's own control tissue -- and if I were able to do so, I certainly would. Not only would it be more cost efficient, but it would also be more "standardized" since I would be the person doing the processing. I agree with that. With regards to a subsequent email that questioned whether or not I should actually be doing the test(s) at all just because I'm looking to purchase control blocks, that's a little insulting. A lot of people purchase control slides for special stains and immunos -- should they not be running those stains just because they don't make their own controls? Why does NSH have an IHC control tissue bank then if that is the case? How does purchasing controls from a company or lab that specifically sells such a product somehow rob me of the knowledge, skill, and expertise necessary to conduct the test? I have made my own monoclonal antibodies in the past via hybridoma production -- does that fact make me any better or more qualified at running an IHC test than someone who purchases their antibodies? No, it doesn't. Anyway, thanks again for the responses. I am also very grateful for the offers that I received to trade blocks with some labs, but I really don't have much to trade at this point...and what I do have, I actually need for myself (i.e. I don't have much to spare). I got a couple of suggestions for companies that actually sell control blocks, so I will look into that. Thanks again. Sherri >From: "Patsy Ruegg" >To: "'Richard Cartun'" , "'Sherri Anderson'" >, > >Subject: RE: [Histonet] IHC control blocks >Date: Mon, 9 Oct 2006 10:06:21 -0600 > >Good point Richard. >If you don't have the control tissue in house perhaps you should not be >doing the test????/ >Patsy > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard >Cartun >Sent: Monday, October 09, 2006 9:25 AM >To: Sherri Anderson; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] IHC control blocks > >Hi Sherri: > >Do you work in a hospital laboratory? If so, you really should prepare >your own control blocks from tissue that you have on site. I am not a >proponent of using someone else's tissue (that has been fixed and >processed differently) for control purposes. > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Sherri Anderson" 10/09/06 10:22 >AM >>> >Good morning everyone! Does anyone know of a company that sells >control >blocks for IHC? I'm looking for blocks rather than slides because 1) I >can >cut my own slides and 2) in the long run, the blocks would probably end >up >being more cost efficient. Does anyone know of such a company? I did >a >quick search on the web but didn't come up with anything. I'm just >looking >for controls for some of the more "routine" antibodies: actin, CEA, >cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. > >Thanks for any feedback! > >Sherri > >_________________________________________________________________ >Try the new Live Search today! >http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLM >TAG > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ Be seen and heard with Windows Live Messenger and Microsoft LifeCams http://clk.atdmt.com/MSN/go/msnnkwme0020000001msn/direct/01/?href=http://www.microsoft.com/hardware/digitalcommunication/default.mspx?locale=en-us&source=hmtagline From llewllew <@t> shaw.ca Mon Oct 9 13:47:35 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Oct 9 13:47:41 2006 Subject: [Histonet] Periodic acid vs environment References: <002201c6ebc6$0f686670$a5060a0a@ipimar.pt> Message-ID: <001e01c6ebd3$63487e80$130e4246@yourlk4rlmsu> There are no silly questions, only things people know or don't know. I have never heard that flushing periodic acid is a problem. I presume it would fairly rapidly encounter reducing agents and lose its excess oxygen, converting to an iodide. There is a shortage of iodine compounds away from the sea (hence historical goitre), so I think the amount flushed would have little effect. Check your local municipal water supply company if you have to know for sure. Bryan Llewellyn ----- Original Message ----- From: "Cristina Nunes" To: Sent: Monday, October 09, 2006 10:12 AM Subject: [Histonet] Periodic acid vs environment > Dear all, > I have been trying for the first time the PAS staining and have a > "practical" problem. How harmful is the periodic acid for the > environment? Is it something you may throw out without any harm or do I > need to store it for a specialised discard? > I hope it is not a very silly question and many thanks for your help. > Best regards, > Cristina Nunes. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From langxingpan <@t> pantomics.com Mon Oct 9 13:50:24 2006 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Mon Oct 9 13:50:28 2006 Subject: [Histonet] RE: IHC control blocks Message-ID: <003301c6ebd3$c94f44c0$4a39c647@Familyroom> Dear Sherri, We have a 'Universal' IHC control tissue array (UNC241, 24 duplicated samples) which covers >90% commonly used IHC markers including all the "routine" makers that you mentioned (please check: http://www.pantomics.com/products/UNC241.htm). There are two different core sizes, 1.5 mm and 1 mm. The 1 mm UNC241 array is designed for 'built-in' control for every slide. Both arrays are being tested in some IHC centers, including NEQAS IHC center in UCL, London, U.K.. If you (or any IHC routine lab) want to try it I can send you some free sample array sections. For more information, please email me. As for paraffin blocks and price, we are in a process of working out an acceptable package for routine IHC lab. The tissue samples for the array have been fixed and processed in our dedicated tissue bank using identical standard protocols. Regards, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107, USA Tel: 1-415-863-2380 Fax: 1-510-653-1227 langxingpan@pantomics.com www.pantomics.com From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherri Anderson Sent: Monday, October 09, 2006 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC control blocks Good morning everyone! Does anyone know of a company that sells control blocks for IHC? I'm looking for blocks rather than slides because 1) I can cut my own slides and 2) in the long run, the blocks would probably end up being more cost efficient. Does anyone know of such a company? I did a quick search on the web but didn't come up with anything. I'm just looking for controls for some of the more "routine" antibodies: actin, CEA, cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. Thanks for any feedback! Sherri From wsimms <@t> mcintoshclinic.com Mon Oct 9 14:21:12 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Mon Oct 9 14:21:26 2006 Subject: [Histonet] STP420 from Richard Allan. . References: <5AEC610C1CE02945BD63A395BA763EDEB1CA61@NVCIEXCH02.NVCI.org> Message-ID: <002f01c6ebd8$15b5b110$530114ac@w3256domain.com> How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Mon Oct 9 14:56:17 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Mon Oct 9 14:56:29 2006 Subject: [Histonet] STP420 from Richard Allan. . In-Reply-To: <002f01c6ebd8$15b5b110$530114ac@w3256domain.com> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A415@SCREECH.ntcampus.smdc.org> Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Maxim_71 <@t> mail.ru Mon Oct 9 14:17:18 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Oct 9 15:14:21 2006 Subject: [Histonet] How much Triton X 100 is OK for permiability during staining Message-ID: <1573580140.20061009231718@mail.ru> Sohail: We really add Tween 20 in stock solutions for some histochemical stains: Hematoxylin, Gram, AFB, Giemsa and others. It is very useful for us. We do not IHC. Maxim Maxim Peshkov, histotechnologist Department of biopsy and cytological research Pathological and anatomical bureau Taganrog, Russia From wsimms <@t> mcintoshclinic.com Mon Oct 9 16:12:54 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Mon Oct 9 16:13:05 2006 Subject: [Histonet] STP420 from Richard Allan. . References: <1A9F2A6C5762524799A816F1F09744CF0143A415@SCREECH.ntcampus.smdc.org> Message-ID: <005801c6ebe7$b097d0f0$530114ac@w3256domain.com> By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From EWURDAK <@t> CSBSJU.EDU Mon Oct 9 16:33:38 2006 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Mon Oct 9 16:33:40 2006 Subject: [Histonet] Periodic acid vs environment In-Reply-To: <002201c6ebc6$0f686670$a5060a0a@ipimar.pt> Message-ID: Hi Cristina, We have been using the PAS staining method for years. We always collect the periodic acid and send it out with the rest of our toxic waste for proper disposal. Don't pour it down the drain. Cheers, Elizabeth Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 10/9/06 12:12 PM, "Cristina Nunes" wrote: > Dear all, > I have been trying for the first time the PAS staining and have a > "practical" problem. How harmful is the periodic acid for the > environment? Is it something you may throw out without any harm or do I > need to store it for a specialised discard? > I hope it is not a very silly question and many thanks for your help. > Best regards, > Cristina Nunes. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lao_ji <@t> yahoo.com Mon Oct 9 17:09:12 2006 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Mon Oct 9 17:09:18 2006 Subject: [Histonet] InsituPro VS Message-ID: <20061009220912.93678.qmail@web30705.mail.mud.yahoo.com> Dear Histoneters, Are there any people having experience in InSituPro VS for slide In Situ or/and for whole mount In Situ? The Lab I am working in is thinking to buy an automated machine for slide In Situ. Any comment is welcome! Thanks! Jimmy __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pruegg <@t> ihctech.net Mon Oct 9 17:08:50 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 9 17:10:14 2006 Subject: [Histonet] IHC control blocks In-Reply-To: Message-ID: <011401c6ebef$80e39f30$6601a8c0@Patsy> Sherri, I am sorry you took my statement as an insult I did not mean that not using your own clinical controls made you any less knowledgeable, I thought that you were perhaps from a clinical setting and was concerned about the results compared to your tissue processing ( my concern was what was best for the patient) if you had a control tissue from outside that was optimally fixed and processed for that antibody and for some reason your in house patient tissue was not, you could possible get a false negative and think it was real because your control tissue worked. One of the very reasons we have the NSH IHC control tissue bank is for "research" just like you are doing and for really hard to find IHC control tissues. We always say that what ever tissue block we send out has to be tested in your environment, we do not validate our tissues in the bank. Please do join the IHCRG, I am sure you will find this group very helpful and happy to share controls or any other resources we have. Best regards, Patsy -----Original Message----- From: Sherri Anderson [mailto:specialstainsqueen@hotmail.com] Sent: Monday, October 09, 2006 12:41 PM To: pruegg@ihctech.net; Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC control blocks Thanks to everyone for their input. I appreciate the information that was provided. To answer Richard's question, I do not work in a clinical hospital laboratory; therefore, I do not have access to a variety of human control tissues. I simply want to validate my own antibody preparations for a project I am researching. I totally understand what you (and others) are saying about preparing one's own control tissue -- and if I were able to do so, I certainly would. Not only would it be more cost efficient, but it would also be more "standardized" since I would be the person doing the processing. I agree with that. With regards to a subsequent email that questioned whether or not I should actually be doing the test(s) at all just because I'm looking to purchase control blocks, that's a little insulting. A lot of people purchase control slides for special stains and immunos -- should they not be running those stains just because they don't make their own controls? Why does NSH have an IHC control tissue bank then if that is the case? How does purchasing controls from a company or lab that specifically sells such a product somehow rob me of the knowledge, skill, and expertise necessary to conduct the test? I have made my own monoclonal antibodies in the past via hybridoma production -- does that fact make me any better or more qualified at running an IHC test than someone who purchases their antibodies? No, it doesn't. Anyway, thanks again for the responses. I am also very grateful for the offers that I received to trade blocks with some labs, but I really don't have much to trade at this point...and what I do have, I actually need for myself (i.e. I don't have much to spare). I got a couple of suggestions for companies that actually sell control blocks, so I will look into that. Thanks again. Sherri >From: "Patsy Ruegg" >To: "'Richard Cartun'" , "'Sherri Anderson'" >, > >Subject: RE: [Histonet] IHC control blocks >Date: Mon, 9 Oct 2006 10:06:21 -0600 > >Good point Richard. >If you don't have the control tissue in house perhaps you should not be >doing the test????/ >Patsy > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard >Cartun >Sent: Monday, October 09, 2006 9:25 AM >To: Sherri Anderson; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] IHC control blocks > >Hi Sherri: > >Do you work in a hospital laboratory? If so, you really should prepare >your own control blocks from tissue that you have on site. I am not a >proponent of using someone else's tissue (that has been fixed and >processed differently) for control purposes. > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Sherri Anderson" 10/09/06 10:22 >AM >>> >Good morning everyone! Does anyone know of a company that sells >control >blocks for IHC? I'm looking for blocks rather than slides because 1) I >can >cut my own slides and 2) in the long run, the blocks would probably end >up >being more cost efficient. Does anyone know of such a company? I did >a >quick search on the web but didn't come up with anything. I'm just >looking >for controls for some of the more "routine" antibodies: actin, CEA, >cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. > >Thanks for any feedback! > >Sherri > >_________________________________________________________________ >Try the new Live Search today! >http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WL M >TAG > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ Be seen and heard with Windows Live Messenger and Microsoft LifeCams http://clk.atdmt.com/MSN/go/msnnkwme0020000001msn/direct/01/?href=http://www .microsoft.com/hardware/digitalcommunication/default.mspx?locale=en-us&sourc e=hmtagline From mtarango <@t> nvcancer.org Mon Oct 9 17:24:44 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Oct 9 17:25:05 2006 Subject: [Histonet] STP420 from Richard Allan. . Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1CAB7@NVCIEXCH02.NVCI.org> You can process at around 5 hours on the STP420. I know that just from working out my processing schedules. They also sell a line of reagents that speed up processing more than conventional reagents, but they aren't required to be used w/ the processor. I haven't tried out the "Fast Flex" reagents yet, but I like having the choice. It DOESN'T cost $250,000 either. If you're using the rotation chamber, the tissue is being rotated through the formalin which speeds up fixation, so that's another bonus. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From TWM-VMD <@t> comcast.net Mon Oct 9 18:24:07 2006 From: TWM-VMD <@t> comcast.net (Thomas Mitchell) Date: Mon Oct 9 18:24:13 2006 Subject: [Histonet] Proof that fixation in formaldehyde solution canbereversible? In-Reply-To: <00ab01c6e7db$38e6ed30$6601a8c0@Patsy> References: <00ab01c6e7db$38e6ed30$6601a8c0@Patsy> Message-ID: <7EAEA21B-526A-4786-BAA6-8D1B4696A35F@comcast.net> One step further with this note. For straight morphology, 24-48 hours of fixation in 10% formaldehyde followed by 70% ethanol, how long do you feel comfortable keeping the tissue in 70% ethanol before processing? If you can?t process for whatever reason, any thoughts on what to keep tissues in until processing occurs? Tom On Oct 4, 2006, at 1:33 PM, Patsy Ruegg wrote: " Fixation is generally carried on by the alcohols used for the processing.? Here in lies the problem for many antigens "unintended consequences" you might say. If the sample is not adequately fixed-proteins protected by crosslinking, the alcohols can destroy the protein of interest. We need to fix for a minimum of 24 hours to allow for crosslinking which will protect the proteins (especially surface proteins) from processing so that they are still there and can be accessed after unmasking the formalin fixation. If certain proteins are not protected from tissue processing they will be lost forever and no amount of epitope retrieval will restore them. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, October 03, 2006 10:49 AM To: GT Hebert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Proof that fixation in formaldehyde solution can be reversible? This is really opening a barrel of worms but... Re formaldehyde solutions. If tissues are fixed for a few hours in standard formalin solutions then it is possible to reverse the fixation process by washing. It is a reversible process in that the bonds that have been formed are generally temporary and can be broken. Technically if you wish for "adequate or true fixation" then Pearse provides the theory for why this should be for about 1 week. (The fixation process does in fact continue for years). Of course no one fixes for one week except by accident. However the fixation for 24 to 48 hours is only an initial fixation. Fixation is generally carried on by the alcohols used for the processing. A. G. E. Pearse "Histochemistry Theoretical and Applied: volume 1 Preparative and Optical Technology."Churchill Livingstone. Barry From raj <@t> bluemarble.net Mon Oct 9 19:28:39 2006 From: raj <@t> bluemarble.net (raj) Date: Mon Oct 9 18:54:01 2006 Subject: [Histonet] Clean area Message-ID: <452AE936.B1692C09@bluemarble.net> What is everyone's feeling on a clean area. We have a room with our slides and blocks(paraffin) No chemicals are in this room. This is a room where we keep our purses, and we all have a section for personal things, such as food or drink. We have always cleaned this out before inspection. After thinking about it. In pathology dept. which is next to our dept they have slides and blocks that they are sending out and receiving. It is ok for food and drink in there. Please advise me about this. Thanks for all your help. Is their a CAP rule for this. I could not find anything. Rebecca A. Johnson From alineumann <@t> aol.com Mon Oct 9 22:41:18 2006 From: alineumann <@t> aol.com (alineumann@aol.com) Date: Mon Oct 9 22:41:31 2006 Subject: [Histonet] Program that works for shave skins and slightly larger ie 1 x 1 x 0.2 cm but not excisions In-Reply-To: <8C8B9E1BC2048A4-90C-1717@FWM-R16.sysops.aol.com> References: <20061007150915.43582.qmail@web61320.mail.yahoo.com> <8C8B9E1BC2048A4-90C-1717@FWM-R16.sysops.aol.com> Message-ID: <8C8BA35149F13E3-C40-512A@WEBMAIL-MA10.sysops.aol.com> Hi we had a problem with fried GI biopsies and used 10 minutes per solution. Skins that are not too thick and that don't have a lot of fat work well with this one below. 12 hours is a long time for small shave biopsies if heat is used. Alice Neumann MD SHANDON HYPERCENTER HP TISSUE PROCESSOR 5 HOUR PROCESS W/VACUUM REAGENTIMMERSION TIMETEMPERATUREVACUUMDRAIN TIME 10% BUFFERED FORMALIN15 MINUTES45 DEGREES C.YES30 SECONDS 10% BUFFERED FORMALIN20 MINUTES45 DEGREES C.YES30 SECONDS 70% ETHYL ALCOHOL20 MINUTES45 DEGREES C.YES30 SECONDS 95% ETHYL ALCOHOL20 MINUTES45 DEGREES C.YES30 SECONDS 95% ETHYL ALCOHOL25 MINUTES45 DEGREES C.YES30 SECONDS 100% ETHYL ALCOHOL20 MINUTES45 DEGREES C.YES30 SECONDS 100% ETHYL ALCOHOL25 MINUTES45 DEGREES C.YES30 SECONDS 100% ETHYL ALCOHOL30 MINUTES45 DEGREES C.YES30 SECONDS XYLENE20 MINUTES45 DEGREES C.YES30 SECONDS XYLENE20 MINUTES45 DEGREES C.YES30 SECONDS PARAFFIN20 MINUTES60 DEGREES CYES30 SECONDS PARAFFIN30 MINUTES60 DEGREES CYES2 MINUTES ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From specialstainsqueen <@t> hotmail.com Tue Oct 10 07:05:55 2006 From: specialstainsqueen <@t> hotmail.com (Sherri Anderson) Date: Tue Oct 10 07:06:02 2006 Subject: [Histonet] IHC control blocks In-Reply-To: <011401c6ebef$80e39f30$6601a8c0@Patsy> Message-ID: Patsy- I also am sorry that I misinterpreted your email...and appreciate the time you've taken to clarify and to explain a little about NSH's IHC tissue control bank. I understand what you are saying about using outside controls if one's own in-house processing of patient tissues is suboptimal...and I agree with you. Hopefully, our exchange has also made some others aware of the fact that NSH has an IHC tissue control bank (I was previously unaware of that myself). Thank you for bringing it to my attention -- I'm sincerely appreciative of your time. Sherri >From: "Patsy Ruegg" >To: "'Sherri Anderson'" , >, >Subject: RE: [Histonet] IHC control blocks >Date: Mon, 9 Oct 2006 16:08:50 -0600 > >Sherri, >I am sorry you took my statement as an insult I did not mean that not using >your own clinical controls made you any less knowledgeable, I thought that >you were perhaps from a clinical setting and was concerned about the >results >compared to your tissue processing ( my concern was what was best for the >patient) if you had a control tissue from outside that was optimally fixed >and processed for that antibody and for some reason your in house patient >tissue was not, you could possible get a false negative and think it was >real because your control tissue worked. >One of the very reasons we have the NSH IHC control tissue bank is for >"research" just like you are doing and for really hard to find IHC control >tissues. We always say that what ever tissue block we send out has to be >tested in your environment, we do not validate our tissues in the bank. >Please do join the IHCRG, I am sure you will find this group very helpful >and happy to share controls or any other resources we have. >Best regards, >Patsy > >-----Original Message----- >From: Sherri Anderson [mailto:specialstainsqueen@hotmail.com] >Sent: Monday, October 09, 2006 12:41 PM >To: pruegg@ihctech.net; Rcartun@harthosp.org; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] IHC control blocks > >Thanks to everyone for their input. I appreciate the information that was >provided. > >To answer Richard's question, I do not work in a clinical hospital >laboratory; therefore, I do not have access to a variety of human control >tissues. I simply want to validate my own antibody preparations for a >project I am researching. I totally understand what you (and others) are >saying about preparing one's own control tissue -- and if I were able to do >so, I certainly would. Not only would it be more cost efficient, but it >would also be more "standardized" since I would be the person doing the >processing. I agree with that. > >With regards to a subsequent email that questioned whether or not I should >actually be doing the test(s) at all just because I'm looking to purchase >control blocks, that's a little insulting. A lot of people purchase >control > >slides for special stains and immunos -- should they not be running those >stains just because they don't make their own controls? Why does NSH have >an IHC control tissue bank then if that is the case? How does purchasing >controls from a company or lab that specifically sells such a product >somehow rob me of the knowledge, skill, and expertise necessary to conduct >the test? I have made my own monoclonal antibodies in the past via >hybridoma production -- does that fact make me any better or more qualified >at running an IHC test than someone who purchases their antibodies? No, it >doesn't. > >Anyway, thanks again for the responses. I am also very grateful for the >offers that I received to trade blocks with some labs, but I really don't >have much to trade at this point...and what I do have, I actually need for >myself (i.e. I don't have much to spare). I got a couple of suggestions >for > >companies that actually sell control blocks, so I will look into that. >Thanks again. > >Sherri > > > > >From: "Patsy Ruegg" > >To: "'Richard Cartun'" , "'Sherri Anderson'" > >, > > > >Subject: RE: [Histonet] IHC control blocks > >Date: Mon, 9 Oct 2006 10:06:21 -0600 > > > >Good point Richard. > >If you don't have the control tissue in house perhaps you should not be > >doing the test????/ > >Patsy > > > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard > >Cartun > >Sent: Monday, October 09, 2006 9:25 AM > >To: Sherri Anderson; histonet@lists.utsouthwestern.edu > >Subject: Re: [Histonet] IHC control blocks > > > >Hi Sherri: > > > >Do you work in a hospital laboratory? If so, you really should prepare > >your own control blocks from tissue that you have on site. I am not a > >proponent of using someone else's tissue (that has been fixed and > >processed differently) for control purposes. > > > >Richard > > > >Richard W. Cartun, Ph.D. > >Director, Immunopathology & Histology > >Assistant Director, Anatomic Pathology > >Hartford Hospital > >80 Seymour Street > >Hartford, CT 06102 > >(860) 545-1596 > >(860) 545-0174 Fax > > > > >>> "Sherri Anderson" 10/09/06 10:22 > >AM >>> > >Good morning everyone! Does anyone know of a company that sells > >control > >blocks for IHC? I'm looking for blocks rather than slides because 1) I > >can > >cut my own slides and 2) in the long run, the blocks would probably end > >up > >being more cost efficient. Does anyone know of such a company? I did > >a > >quick search on the web but didn't come up with anything. I'm just > >looking > >for controls for some of the more "routine" antibodies: actin, CEA, > >cytokeratin, AFP, desmin, GFAP, S-100, chromogranin, PSA, etc. > > > >Thanks for any feedback! > > > >Sherri > > > >_________________________________________________________________ > >Try the new Live Search today! > >http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WL >M > >TAG > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_________________________________________________________________ >Be seen and heard with Windows Live Messenger and Microsoft LifeCams >http://clk.atdmt.com/MSN/go/msnnkwme0020000001msn/direct/01/?href=http://www >.microsoft.com/hardware/digitalcommunication/default.mspx?locale=en-us&sourc >e=hmtagline > _________________________________________________________________ Try the new Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&FORM=WLMTAG From rjbuesa <@t> yahoo.com Tue Oct 10 09:14:08 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 09:14:14 2006 Subject: [Histonet] Clean area In-Reply-To: <452AE936.B1692C09@bluemarble.net> Message-ID: <20061010141408.23531.qmail@web61218.mail.yahoo.com> We always had a designated area with just: lockers, a table, chairs, some wall cabinets, a refrigerator and a MW oven. Nothing else. In this room we had lunch and other breaks. Any area with anything coming from the lab, no matter how innocuous it may seem, is not a "clean area". Ren? J. raj wrote: What is everyone's feeling on a clean area. We have a room with our slides and blocks(paraffin) No chemicals are in this room. This is a room where we keep our purses, and we all have a section for personal things, such as food or drink. We have always cleaned this out before inspection. After thinking about it. In pathology dept. which is next to our dept they have slides and blocks that they are sending out and receiving. It is ok for food and drink in there. Please advise me about this. Thanks for all your help. Is their a CAP rule for this. I could not find anything. Rebecca A. Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From aep10 <@t> cornell.edu Tue Oct 10 09:19:06 2006 From: aep10 <@t> cornell.edu (Anna Elisse Beaudin) Date: Tue Oct 10 09:19:16 2006 Subject: [Histonet] embedding/freezing samples from glycerol Message-ID: <1640.132.236.104.213.1160489946.squirrel@webmail.cornell.edu> Hello, I recently froze embryo samples in OCT that had been sitting in 70% glycerol. When I tried to section these samples on the cryostat, the results were terrible -- the specimen basically would not be cut (appeared gooey and stuck out of the block). I wasn't able to save anything. The problem I have is that all of my samples are in glycerol, and I would eventually like to section them. :) Does anyone have any advice as to what I can do with these samples to make them section-able? They have been stained with Xgal, so I can't do anything that would weaken the stain. Thanks in advance for any advice! Best, Anna Beaudin Division of Nutritional Sciences Cornell University From rjbuesa <@t> yahoo.com Tue Oct 10 09:32:31 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 09:32:49 2006 Subject: [Histonet] STP420 from Richard Allan. . In-Reply-To: <005801c6ebe7$b097d0f0$530114ac@w3256domain.com> Message-ID: <20061010143231.69955.qmail@web61212.mail.yahoo.com> Dr. Simms: According with Morales et al. ("inventors") you can process tissue in the Xpress that has not been previously fixed, now it turns out that you cannot? That is another drawback yet, because it is also not true that you will be able to process 120 cassettes in 1 hour; their mathematics do not add up. The protocol (2 stations with MW assisted protocol) + 2 "conventional" stations (just convection heating + vacuum) takes 60 minutes to complete (15 min each). The only real advantage seems to be that you can introduce 30 cassettes every 15 minutes in the first station. This means that you will have 30 cassettes processed in 1 hour, the following 30 (to a total of 60) the next 15 minutes (=75 minutes) and so on until you get to the 120 cassettes after 105 minutes. And all of this has nothing to do, has not impacted at all, on the time required for manual work (grossing/cassetting, embedding, trimming and sectioning the blocks) that will amount to a total of 149 minuttes per every 30 cassettes, even allowing that there will be automated instruments to write cassetes, etch slides, routine stain and coverslip. The "famous" 120 cassettes / hour turn out to be a total of 105 min. processing + 160 minutes of automated ancillary tasks + 596 minutes of manual work, for a grand total of 861 min =14.4 h / 120 cassettes = 2.4 FTE with 75% time utilization on 8h shifts! I could also buy a good house with $250,000! Just a few "numbers" about the Xpress (no reagents cost included of course!) Ren? J. "Wesley Simms, MD" wrote: By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From settembr <@t> umdnj.edu Tue Oct 10 09:47:05 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Oct 10 09:48:15 2006 Subject: [Histonet] EGFR vIII antibody? Message-ID: Does anyone know of a commercial source for EGFR vIII antibody? We want to work it up on formalin fixed paraffin embedded human tissue. Thank you. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wellborj <@t> mercyhealth.com Tue Oct 10 09:53:09 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Tue Oct 10 09:53:43 2006 Subject: [Histonet] Ventana and Benchmark Maintance Message-ID: All, 1st - I want to thank everyone who has responded to my previous posting. I have been in a process of discovery about my training on the Ventana and Benchmark. This instutution is the 1st one that I have worked at that had this intrumentation. As we all know at times - people can be completely misled. No - our procedures do not completely outline all of the steps of the required maitance - it just provideds a list of the tasks without explaining how to do it. If someone could please send me a copy of yours I would truely appreciate it. I just want to be able to do my job correctly and to be able to trust my knowledge. Thank-you in advance! Janci Wellborn, HTL (ASCP), BS, BSeD From c.thompson <@t> student.ucc.ie Tue Oct 10 09:56:13 2006 From: c.thompson <@t> student.ucc.ie (c.thompson@student.ucc.ie) Date: Tue Oct 10 09:59:13 2006 Subject: [Histonet] Re: Immunofluorescence for rat kidney Message-ID: <452bb48d.e7.6d8a.1472671302@student.ucc.ie> Just wondering if anyone could provide me with an immunofluorescence protocol for tissue. Am trying to localise NHE3 in the rat kidney. Don't know whether the protocol I'm using is appropriate so just wanted to check if anyone knew of anything specific to kidney? Would be extremely grateful for any help at all. Thank you, Claire From wsimms <@t> mcintoshclinic.com Tue Oct 10 10:14:34 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Tue Oct 10 10:14:57 2006 Subject: [Histonet] STP420 from Richard Allan. . References: <20061010143231.69955.qmail@web61212.mail.yahoo.com> Message-ID: <002201c6ec7e$cc0434e0$530114ac@w3256domain.com> Rene, Yes indeed, I was told last week by the nice pathologist from Miami (whose name eludes me at the moment) who Sakura brought in to teach us to use the new grossing tools that raw tissue, even if sliced at 1.5mm thick, could not be put on the machine. He quoted the 4 hour figure for breast tissue in particular. When I saw the graph of Miami's FTE staffing for HT's and pathologists by time of day (before and after Xpress), I was also puzzled as to how this would translate into any tangible benefit, either cost-wise or by improved patient care. The only result I could see was that everyone's day is going to start later and be strung out over a longer period of time. My wife, who is an attorney, summed it up best:"At least with the old processor, something was doing work overnight and being productive. Now, with the new one, nothing is." Wesley Simms ----- Original Message ----- From: Rene J Buesa To: Wesley Simms, MD ; histonet@lists.utsouthwestern.edu Sent: Tuesday, October 10, 2006 10:32 AM Subject: Re: [Histonet] STP420 from Richard Allan. . Dr. Simms: According with Morales et al. ("inventors") you can process tissue in the Xpress that has not been previously fixed, now it turns out that you cannot? That is another drawback yet, because it is also not true that you will be able to process 120 cassettes in 1 hour; their mathematics do not add up. The protocol (2 stations with MW assisted protocol) + 2 "conventional" stations (just convection heating + vacuum) takes 60 minutes to complete (15 min each). The only real advantage seems to be that you can introduce 30 cassettes every 15 minutes in the first station. This means that you will have 30 cassettes processed in 1 hour, the following 30 (to a total of 60) the next 15 minutes (=75 minutes) and so on until you get to the 120 cassettes after 105 minutes. And all of this has nothing to do, has not impacted at all, on the time required for manual work (grossing/cassetting, embedding, trimming and sectioning the blocks) that will amount to a total of 149 minuttes per every 30 cassettes, even allowing that there will be automated instruments to write cassetes, etch slides, routine stain and coverslip. The "famous" 120 cassettes / hour turn out to be a total of 105 min. processing + 160 minutes of automated ancillary tasks + 596 minutes of manual work, for a grand total of 861 min =14.4 h / 120 cassettes = 2.4 FTE with 75% time utilization on 8h shifts! I could also buy a good house with $250,000! Just a few "numbers" about the Xpress (no reagents cost included of course!) Ren? J. "Wesley Simms, MD" wrote: By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From GDawson <@t> dynacaremilwaukee.com Tue Oct 10 10:32:00 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Oct 10 10:32:11 2006 Subject: [Histonet] Antibody Search Message-ID: All, Does anyone have a good source for an antibody called "protoplanin" or "D240" or both of these names for use on FFPE tissues? I have a Pathologist that is foaming at the mouth for this one. Thank-you in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From rjbuesa <@t> yahoo.com Tue Oct 10 10:32:19 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 10:32:31 2006 Subject: [Histonet] STP420 from Richard Allan. . In-Reply-To: <002201c6ec7e$cc0434e0$530114ac@w3256domain.com> Message-ID: <20061010153219.37983.qmail@web61219.mail.yahoo.com> Wesley: As you perfectly know, tissue processors "liberated" the histotech from the "slavery" of hand processing but we still have to see another "liberations". SakuraFineTek now has also a piece of equipment for automated embedding that has so many restrictions regarding the initial cassetting and has so many expensive complements that I doubt "will fly" specially because the cassettes are made of a plastic that has to be sectioned ALONG with the tissue. I have not hear histotechs screaming about that yet because I don't think they have been able to sell it. On the other hand, with regards to MW assisted technology for tissue processing the only really convenient are those with the "walk away" capability and so far only the PATHOS from Milestone and the Xpress from Sakura FineTek provide it. Any other single chamber MW oven that needs the handling of the cassettes by the histotech from one station to the other is just returning to old "slavery" days. MW assisted tissue processing is something to take with several, not just one grain of salt. On the other hand if you are able to process tissue quickly just to be sitting before sectioning, makes no sense. MW assisted tissue processing without rescheduling is a waste of resources, as are those MW tissue processors with 180 blocks capabilities: to get to such a large batch you will need several hours of grossing / casseting and what are you going to do?: start the processor at 4 PM and start sectioning at 7PM through the night? Promote an even worse histotech slavery? I don't think so. This whole issue has to be analyzed cautiously; this is not a band wagon to jump into without thinking it well! Just my opinion though! Ren? J. "Wesley Simms, MD" wrote: Rene, Yes indeed, I was told last week by the nice pathologist from Miami (whose name eludes me at the moment) who Sakura brought in to teach us to use the new grossing tools that raw tissue, even if sliced at 1.5mm thick, could not be put on the machine. He quoted the 4 hour figure for breast tissue in particular. When I saw the graph of Miami's FTE staffing for HT's and pathologists by time of day (before and after Xpress), I was also puzzled as to how this would translate into any tangible benefit, either cost-wise or by improved patient care. The only result I could see was that everyone's day is going to start later and be strung out over a longer period of time. My wife, who is an attorney, summed it up best:"At least with the old processor, something was doing work overnight and being productive. Now, with the new one, nothing is." Wesley Simms ----- Original Message ----- From: Rene J Buesa To: Wesley Simms, MD ; histonet@lists.utsouthwestern.edu Sent: Tuesday, October 10, 2006 10:32 AM Subject: Re: [Histonet] STP420 from Richard Allan. . Dr. Simms: According with Morales et al. ("inventors") you can process tissue in the Xpress that has not been previously fixed, now it turns out that you cannot? That is another drawback yet, because it is also not true that you will be able to process 120 cassettes in 1 hour; their mathematics do not add up. The protocol (2 stations with MW assisted protocol) + 2 "conventional" stations (just convection heating + vacuum) takes 60 minutes to complete (15 min each). The only real advantage seems to be that you can introduce 30 cassettes every 15 minutes in the first station. This means that you will have 30 cassettes processed in 1 hour, the following 30 (to a total of 60) the next 15 minutes (=75 minutes) and so on until you get to the 120 cassettes after 105 minutes. And all of this has nothing to do, has not impacted at all, on the time required for manual work (grossing/cassetting, embedding, trimming and sectioning the blocks) that will amount to a total of 149 minuttes per every 30 cassettes, even allowing that there will be automated instruments to write cassetes, etch slides, routine stain and coverslip. The "famous" 120 cassettes / hour turn out to be a total of 105 min. processing + 160 minutes of automated ancillary tasks + 596 minutes of manual work, for a grand total of 861 min =14.4 h / 120 cassettes = 2.4 FTE with 75% time utilization on 8h shifts! I could also buy a good house with $250,000! Just a few "numbers" about the Xpress (no reagents cost included of course!) Ren? J. "Wesley Simms, MD" wrote: By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. --------------------------------- Get your email and more, right on the new Yahoo.com From anh2006 <@t> med.cornell.edu Tue Oct 10 10:44:18 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Oct 10 10:44:32 2006 Subject: [Histonet] Antibody Search In-Reply-To: References: Message-ID: I believe Novus (and apparently Genetex from a quick search) sells D240. I am sure there are others. I thought DAKO sold it but I cannot find it listed in their website .......... Also I believe Developmental Studies Hybridoma Bank sells anti-podoplanin polyclonal which is good - I have used that one. Generally speaking (if your pathologist is interested in lymphatics), I am not a huge fan of using podoplanin as it isn't all that specific for lymphatics ... There are better markers. >All, > >Does anyone have a good source for an antibody called "protoplanin" >or "D240" or both of these names for use on FFPE tissues? I have a >Pathologist that is foaming at the mouth for this one. > >Thank-you in Advance, > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From JWEEMS <@t> sjha.org Tue Oct 10 10:44:31 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Oct 10 10:44:49 2006 Subject: [Histonet] STP420 from Richard Allan. . Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED8F@sjhaexc02.sjha.org> Just a reminder that tissue to be used for Her2/Neu needs to be adequately fixed in formalin in order to meet FDA criteria. And, from my research and investigation into the Xpress, I found it be excellent instrument - best suited for 24 hour labs that have thousands and thousands of specimens. I believe some 24 hour labs are able to shorten their days and have found a reduction in FTEs with the faster processing. It is a big change to adjust to! Again my 2 cents... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Tuesday, October 10, 2006 11:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . Rene, Yes indeed, I was told last week by the nice pathologist from Miami (whose name eludes me at the moment) who Sakura brought in to teach us to use the new grossing tools that raw tissue, even if sliced at 1.5mm thick, could not be put on the machine. He quoted the 4 hour figure for breast tissue in particular. When I saw the graph of Miami's FTE staffing for HT's and pathologists by time of day (before and after Xpress), I was also puzzled as to how this would translate into any tangible benefit, either cost-wise or by improved patient care. The only result I could see was that everyone's day is going to start later and be strung out over a longer period of time. My wife, who is an attorney, summed it up best:"At least with the old processor, something was doing work overnight and being productive. Now, with the new one, nothing is." Wesley Simms ----- Original Message ----- From: Rene J Buesa To: Wesley Simms, MD ; histonet@lists.utsouthwestern.edu Sent: Tuesday, October 10, 2006 10:32 AM Subject: Re: [Histonet] STP420 from Richard Allan. . Dr. Simms: According with Morales et al. ("inventors") you can process tissue in the Xpress that has not been previously fixed, now it turns out that you cannot? That is another drawback yet, because it is also not true that you will be able to process 120 cassettes in 1 hour; their mathematics do not add up. The protocol (2 stations with MW assisted protocol) + 2 "conventional" stations (just convection heating + vacuum) takes 60 minutes to complete (15 min each). The only real advantage seems to be that you can introduce 30 cassettes every 15 minutes in the first station. This means that you will have 30 cassettes processed in 1 hour, the following 30 (to a total of 60) the next 15 minutes (=75 minutes) and so on until you get to the 120 cassettes after 105 minutes. And all of this has nothing to do, has not impacted at all, on the time required for manual work (grossing/cassetting, embedding, trimming and sectioning the blocks) that will amount to a total of 149 minuttes per every 30 cassettes, even allowing that there will be automated instruments to write cassetes, etch slides, routine stain and coverslip. The "famous" 120 cassettes / hour turn out to be a total of 105 min. processing + 160 minutes of automated ancillary tasks + 596 minutes of manual work, for a grand total of 861 min =14.4 h / 120 cassettes = 2.4 FTE with 75% time utilization on 8h shifts! I could also buy a good house with $250,000! Just a few "numbers" about the Xpress (no reagents cost included of course!) Ren? J. "Wesley Simms, MD" wrote: By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Tue Oct 10 10:56:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 10:57:02 2006 Subject: [Histonet] STP420 from Richard Allan. . In-Reply-To: Message-ID: <20061010155652.15228.qmail@web61222.mail.yahoo.com> Karen: That is exactly what I was referring to. There is no way that adding something hard enough to survive the process that is able to keep pushed down the tissue is going to cut "just as if it did not exist". On the other hand, if you are going to use the Xpress along with this automated embedding instrument, you have to change the cassettes holders and reduce them to just 20 per run which means cutting the throughput Xpress capability by 34% (to just 80 cassettes / 105 minutes). Ren? J. "Heckford, Karen - SMMC-SF" wrote: I have actually tried the new cassette that you afe suppose to cut the plastic with the tissue. It was so absolutely horrible I had to laugh. What will they think of next? Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Tuesday, October 10, 2006 8:32 AM To: Wesley Simms, MD; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . Wesley: As you perfectly know, tissue processors "liberated" the histotech from the "slavery" of hand processing but we still have to see another "liberations". SakuraFineTek now has also a piece of equipment for automated embedding that has so many restrictions regarding the initial cassetting and has so many expensive complements that I doubt "will fly" specially because the cassettes are made of a plastic that has to be sectioned ALONG with the tissue. I have not hear histotechs screaming about that yet because I don't think they have been able to sell it. On the other hand, with regards to MW assisted technology for tissue processing the only really convenient are those with the "walk away" capability and so far only the PATHOS from Milestone and the Xpress from Sakura FineTek provide it. Any other single chamber MW oven that needs the handling of the cassettes by the histotech from one station to the other is just returning to old "slavery" days. MW assisted tissue processing is something to take with several, not just one grain of salt. On the other hand if you are able to process tissue quickly just to be sitting before sectioning, makes no sense. MW assisted tissue processing without rescheduling is a waste of resources, as are those MW tissue processors with 180 blocks capabilities: to get to such a large batch you will need several hours of grossing / casseting and what are you going to do?: start the processor at 4 PM and start sectioning at 7PM through the night? Promote an even worse histotech slavery? I don't think so. This whole issue has to be analyzed cautiously; this is not a band wagon to jump into without thinking it well! Just my opinion though! Ren? J. "Wesley Simms, MD" wrote: Rene, Yes indeed, I was told last week by the nice pathologist from Miami (whose name eludes me at the moment) who Sakura brought in to teach us to use the new grossing tools that raw tissue, even if sliced at 1.5mm thick, could not be put on the machine. He quoted the 4 hour figure for breast tissue in particular. When I saw the graph of Miami's FTE staffing for HT's and pathologists by time of day (before and after Xpress), I was also puzzled as to how this would translate into any tangible benefit, either cost-wise or by improved patient care. The only result I could see was that everyone's day is going to start later and be strung out over a longer period of time. My wife, who is an attorney, summed it up best:"At least with the old processor, something was doing work overnight and being productive. Now, with the new one, nothing is." Wesley Simms ----- Original Message ----- From: Rene J Buesa To: Wesley Simms, MD ; histonet@lists.utsouthwestern.edu Sent: Tuesday, October 10, 2006 10:32 AM Subject: Re: [Histonet] STP420 from Richard Allan. . Dr. Simms: According with Morales et al. ("inventors") you can process tissue in the Xpress that has not been previously fixed, now it turns out that you cannot? That is another drawback yet, because it is also not true that you will be able to process 120 cassettes in 1 hour; their mathematics do not add up. The protocol (2 stations with MW assisted protocol) + 2 "conventional" stations (just convection heating + vacuum) takes 60 minutes to complete (15 min each). The only real advantage seems to be that you can introduce 30 cassettes every 15 minutes in the first station. This means that you will have 30 cassettes processed in 1 hour, the following 30 (to a total of 60) the next 15 minutes (=75 minutes) and so on until you get to the 120 cassettes after 105 minutes. And all of this has nothing to do, has not impacted at all, on the time required for manual work (grossing/cassetting, embedding, trimming and sectioning the blocks) that will amount to a total of 149 minuttes per every 30 cassettes, even allowing that there will be automated instruments to write cassetes, etch slides, routine stain and coverslip. The "famous" 120 cassettes / hour turn out to be a total of 105 min. processing + 160 minutes of automated ancillary tasks + 596 minutes of manual work, for a grand total of 861 min =14.4 h / 120 cassettes = 2.4 FTE with 75% time utilization on 8h shifts! I could also buy a good house with $250,000! Just a few "numbers" about the Xpress (no reagents cost included of course!) Ren? J. "Wesley Simms, MD" wrote: By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From vbaker60 <@t> yahoo.com Tue Oct 10 11:11:09 2006 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Tue Oct 10 11:11:15 2006 Subject: [Histonet] unscribe Message-ID: <20061010161109.7296.qmail@web56814.mail.re3.yahoo.com> Hope I did this right! No flaming please __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kappeler <@t> patho.unibe.ch Tue Oct 10 11:19:39 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Oct 10 11:20:21 2006 Subject: [Histonet] Antibody Search References: Message-ID: <012d01c6ec87$f4998640$27955c82@patho.unibe.ch> Hi Glen it's "Podoplanin", clone D2-40. The antibody is available from various sources, including ID Labs (CatNo BP2721), Signet (730-01), Zymed (??), Dako (M3619) and probably more. The antibody works very nicely on FFPE after HIER (e.g. in 1 mM EDTA - 10 mM Tris, pH 9.0). Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Dawson, Glen" To: Sent: Tuesday, October 10, 2006 5:32 PM Subject: [Histonet] Antibody Search > All, > > Does anyone have a good source for an antibody called "protoplanin" or > "D240" or both of these names for use on FFPE tissues? I have a > Pathologist that is foaming at the mouth for this one. > > Thank-you in Advance, > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From benoit.delatour <@t> ibaic.u-psud.fr Tue Oct 10 11:54:22 2006 From: benoit.delatour <@t> ibaic.u-psud.fr (Delatour =?iso-8859-1?Q?Beno=EEt?=) Date: Tue Oct 10 11:54:37 2006 Subject: [Histonet] congo red featuring thioflavin-S Message-ID: <6.0.1.1.2.20061010184437.029ee2d0@pop3.club-internet.fr> Dear Histoneters, Does anyone knows the true difference (in terms of specificity/sensitivity) between Congo red and Thioflavin stains for amyloid deposits (eg Abeta plaques in the brain of Alzheimer's patients or genetically-modified mice that develop cerebral amyloidosis). In a recent publication I used Congo red to quantify agregated Abeta deposits but one of the reviewers claimed that thioflavin was, per sure, more efficient "to provide high contrast to detect even the smallest plaques". From my personal experience I would not share the same conclusions. Has anyone has a bibliographic reference dealing with differences between the two stains? Thanks! Beno?t From Xilong.Li <@t> UTSouthwestern.edu Tue Oct 10 12:01:57 2006 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Tue Oct 10 12:02:14 2006 Subject: [Histonet] looking for a method to statistic analysis HE result Message-ID: <452B8BB5020000F0000082FA@swnw124.swmed.edu> Hi, All, I am looking for a consistent method to do analysis for the results coming from HE staining of muscle tissue. I just wonder is there a method or scale to describe the damage in muslce after HE staining? I need focus on the changes of size of muscle cell, or the percent of area of necrosis, or I can combine those two factors together? How can I describle the damage in muscle in HE staining? Any suggestions would be appreciated. Thanks. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From rjbuesa <@t> yahoo.com Tue Oct 10 12:11:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 12:11:59 2006 Subject: [Histonet] congo red featuring thioflavin-S In-Reply-To: <6.0.1.1.2.20061010184437.029ee2d0@pop3.club-internet.fr> Message-ID: <20061010171154.38941.qmail@web61222.mail.yahoo.com> Neither would I, and you can add that thiflavin preparations will fade and are not permanent, something to consider also when deciding on the procedure. Ren? J. Delatour Beno?t wrote: Dear Histoneters, Does anyone knows the true difference (in terms of specificity/sensitivity) between Congo red and Thioflavin stains for amyloid deposits (eg Abeta plaques in the brain of Alzheimer's patients or genetically-modified mice that develop cerebral amyloidosis). In a recent publication I used Congo red to quantify agregated Abeta deposits but one of the reviewers claimed that thioflavin was, per sure, more efficient "to provide high contrast to detect even the smallest plaques". From my personal experience I would not share the same conclusions. Has anyone has a bibliographic reference dealing with differences between the two stains? Thanks! Beno?t _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From LSebree <@t> uwhealth.org Tue Oct 10 12:15:55 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 10 12:16:01 2006 Subject: [Histonet] Ventana and Benchmark Maintance In-Reply-To: Message-ID: Your on-line manual should give you step-by-step instructions for monthly and quarterly cleaning and maintenance for each instrument you have; our does. We printed the relevant pages out to keep in a binder for our student worker that does these tasks. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janci Wellborn Sent: Tuesday, October 10, 2006 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana and Benchmark Maintance All, 1st - I want to thank everyone who has responded to my previous posting. I have been in a process of discovery about my training on the Ventana and Benchmark. This instutution is the 1st one that I have worked at that had this intrumentation. As we all know at times - people can be completely misled. No - our procedures do not completely outline all of the steps of the required maitance - it just provideds a list of the tasks without explaining how to do it. If someone could please send me a copy of yours I would truely appreciate it. I just want to be able to do my job correctly and to be able to trust my knowledge. Thank-you in advance! Janci Wellborn, HTL (ASCP), BS, BSeD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Tue Oct 10 12:28:20 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Tue Oct 10 12:44:34 2006 Subject: [Histonet] congo red featuring thioflavin-S Message-ID: Congo red is considerd to be more specific, Thiofalavin stains a variety of tissue componets Rena Fail >>> Delatour Beno?t 10/10/06 12:54PM >>> Dear Histoneters, Does anyone knows the true difference (in terms of specificity/sensitivity) between Congo red and Thioflavin stains for amyloid deposits (eg Abeta plaques in the brain of Alzheimer's patients or genetically-modified mice that develop cerebral amyloidosis). In a recent publication I used Congo red to quantify agregated Abeta deposits but one of the reviewers claimed that thioflavin was, per sure, more efficient "to provide high contrast to detect even the smallest plaques". From my personal experience I would not share the same conclusions. Has anyone has a bibliographic reference dealing with differences between the two stains? Thanks! Beno?t _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Tue Oct 10 12:54:52 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Oct 10 12:55:06 2006 Subject: [Histonet] Automated special stains In-Reply-To: Message-ID: <000601c6ec95$320d6a20$db01a8c0@plab.local> I love it! Easy to operate and maintain very stable and reliable. I don't have one here and I miss it! Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCID) Sent: Friday, September 29, 2006 5:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated special stains Sensitivity: Confidential Hi all, Any feedback on the NexES special stains machine? Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Tue Oct 10 12:58:02 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Oct 10 12:58:16 2006 Subject: [Histonet] congo red featuring thioflavin-S References: <20061010171154.38941.qmail@web61222.mail.yahoo.com> Message-ID: <005101c6ec95$a23592a0$6500a8c0@mainbox> While fluorescence of Thioflavine-T(NOT Thioflavine-S) can provide a high contrast for demonstration of small amyloid deposits, providing that the excitation/barrier filter set is optimized to obtain a fully dark field, the staining per se is not specific for amyloid (other basophilic structures can stain even at low pH). Neither is Congo red however, the dichroism and green/red birefringence is specific for B pleated sheets. A carefully optimized polarization microscope, using a monocular head with stress free optics (no tape coverslips!!) to provide a completely dark field, is incredibly sensitive and will reveal even fine reticular fibers coated with amyloid. A literature search will provide several papers comparing the two methods (check Burns 1967, Mowry&Scott 1967, Puchler 1983). Bryan ----- Original Message ----- From: "Rene J Buesa" To: "Delatour Beno?t" ; Sent: Tuesday, October 10, 2006 1:11 PM Subject: Re: [Histonet] congo red featuring thioflavin-S > Neither would I, and you can add that thiflavin preparations will fade and > are not permanent, something to consider also when deciding on the > procedure. > Ren? J. > > Delatour Beno?t wrote: > > Dear Histoneters, > > Does anyone knows the true difference (in terms of > specificity/sensitivity) > between Congo red and Thioflavin stains for amyloid deposits (eg Abeta > plaques in the brain of Alzheimer's patients or genetically-modified mice > that develop cerebral amyloidosis). In a recent publication I used Congo > red to quantify agregated Abeta deposits but one of the reviewers claimed > that thioflavin was, per sure, more efficient "to provide high contrast to > detect even the smallest plaques". From my personal experience I would not > share the same conclusions. Has anyone has a bibliographic reference > dealing with differences between the two stains? > Thanks! > > Beno?t > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Do you Yahoo!? > Get on board. You're invited to try the new Yahoo! Mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Tue Oct 10 13:05:16 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Oct 10 13:05:54 2006 Subject: [Histonet] Patient consent Message-ID: <452BA89D0200007700002557@hcnwgwds01.hh.chs> One of my colleagues wants to send a difficult case to an expert pathologist in Toronto for a second opinion. She is requiring the patient's consent before she accepts the case. How many of you get the patient's consent before sending a case out for second opinion? Is this a HIPAA requirement that I am not familiar with? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From wsimms <@t> mcintoshclinic.com Tue Oct 10 13:30:29 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Tue Oct 10 13:31:10 2006 Subject: [Histonet] Patient consent References: <452BA89D0200007700002557@hcnwgwds01.hh.chs> Message-ID: <006901c6ec9a$30ffff80$530114ac@w3256domain.com> A) With HIPAA, the power of requiring consent rests with the patient, not the caregivers. B) Since the consultant pathologist is Canadian, she is not bound by HIPAA anyway. Wesley W. Simms, M.D. ----- Original Message ----- From: "Richard Cartun" To: Sent: Tuesday, October 10, 2006 2:05 PM Subject: [Histonet] Patient consent > One of my colleagues wants to send a difficult case to an expert > pathologist in Toronto for a second opinion. She is requiring the > patient's consent before she accepts the case. How many of you get the > patient's consent before sending a case out for second opinion? Is this > a HIPAA requirement that I am not familiar with? Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From GDawson <@t> dynacaremilwaukee.com Tue Oct 10 13:35:57 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Oct 10 13:36:07 2006 Subject: [Histonet] Antibody Search Message-ID: Wow, Everyone and their sister seems to sell the prodoplanin D2-40 antibody!! Thanx again to all who responded...you guys rock. Glen Dawson Milwaukee, WI From pkarlisch <@t> psu.edu Tue Oct 10 13:48:49 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Tue Oct 10 13:49:19 2006 Subject: [Histonet] Antigenicity Message-ID: <452BB2D10200008C0002870D@GWIA02.HERSHEYMED.NET> Hello all, I have a technical question. Do any of you pre-cut your control slides in larger batches? It used to be that the control slides would lose their antigenicity if stored for long periods of time (several months) in a slide file as opposed to cutting the blocks as needed. Have the HIER techniques evolved to the point that this is no longer true? Thank you in advance. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From judi.ford <@t> jax.org Tue Oct 10 13:53:14 2006 From: judi.ford <@t> jax.org (judi.ford@jax.org) Date: Tue Oct 10 13:53:27 2006 Subject: [Histonet] Ventana Discovery and XMatrx Message-ID: <27938164.1160506394047.JavaMail.ocsadmin@jcs-mid-prod.jax.org> I was wondering if anyone out there has experience working with the Ventana Discovery and/or XMatrx automated IHC stainers. I work in a research lab with mouse tissue and would love to hear what others have experienced using these machines in similar settings. Have you worked up in situ protocols with dig labeled probes? Have you used the companies reagents or do you use your own? What problems have you encountered? Thanks in advance for any replies....... Judi Ford HIstotechnologist The Jackson Laboratory From cbass <@t> bidmc.harvard.edu Tue Oct 10 13:56:46 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Tue Oct 10 13:56:58 2006 Subject: [Histonet] buffer for mounting floating brain sections Message-ID: <50479E31-CAC2-41A0-AEB0-A907BE636E34@bidmc.harvard.edu> Hey guys, I know this is a question that has probably popped up before, but I searched the archives and didn't find exactly what I need. I want to mount floating mouse brain sections (40 micron, NBF fixed, sucrose protected) onto superfrost slides. I will immediately coverslip after drying, they will not be processed further. What is the best buffer to float the sections in when mounting onto the slides. PBS seems to leave a lot of crystals, I have heard that 0.1M phosphate buffer is better. Distilled water results in a lot of curls. Since I am not going to process the slides I don't have to worry about them falling off later (which I heard is a problem with charged slides and PBS). If there is a preferred buffer could you also include the recipe. Thanks, Caroline From Kari.Zajic <@t> HCAhealthcare.com Tue Oct 10 13:58:17 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Oct 10 13:58:24 2006 Subject: [Histonet] supply charges for frozens Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC73C@ORLEV03.hca.corpad.net> Hello fellow netters! I have posed the "what do I charge for technical help" on frozens but now I need the supply cost. How would you break down a cost per case, for slides, OCT, blades, towels, cassettes, Cytocool, ink, etc.? We're talking like 10-12 blocks frozen in a month's time (for an outside surgicenter skin case) using our hospital supplies. We are trying to draw up a contract to charge the Pathologist for using our services. Any suggestions? Thanks in advance! Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor From rjbuesa <@t> yahoo.com Tue Oct 10 14:03:49 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 14:03:59 2006 Subject: [Histonet] Antigenicity In-Reply-To: <452BB2D10200008C0002870D@GWIA02.HERSHEYMED.NET> Message-ID: <20061010190349.54603.qmail@web61211.mail.yahoo.com> Patricia: Not at all! Antigenicity (due to antigen oxidation) is slowly lost when the control slides are stored for more than 4 weeks, although not all antigens oxidize at the same rate, some being more "resilient" than others. Some experiments have been done about how to prevent this "fading" and they go from storing the contols at 20?C, or in a nitrogen atmosphere, or covering them with paraffin. I did use to put them in a container with mineral oil which isolated them from the air (although is somewhat messy and will prevent you to put the case section along with the control in the same slide). Hope this will help you! Ren? J. Patricia Karlisch wrote: Hello all, I have a technical question. Do any of you pre-cut your control slides in larger batches? It used to be that the control slides would lose their antigenicity if stored for long periods of time (several months) in a slide file as opposed to cutting the blocks as needed. Have the HIER techniques evolved to the point that this is no longer true? Thank you in advance. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From rjbuesa <@t> yahoo.com Tue Oct 10 14:09:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 10 14:09:14 2006 Subject: [Histonet] supply charges for frozens In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC73C@ORLEV03.hca.corpad.net> Message-ID: <20061010190909.75524.qmail@web61222.mail.yahoo.com> Do as I did: observe yourself (or somebody else for that matter) doing the FS and write down all things used. Do it repeatedly (as many times as possible); obtain an average usage for each item and then go to the prices you pay for each item as you purchase ut, extrapolate to your actual usage rate and there you have it: $/slide or $/procedure. Hope this will help you. Under separate cover I am sending you an article on costs I just finished. Ren? J. Zajic Kari wrote: Hello fellow netters! I have posed the "what do I charge for technical help" on frozens but now I need the supply cost. How would you break down a cost per case, for slides, OCT, blades, towels, cassettes, Cytocool, ink, etc.? We're talking like 10-12 blocks frozen in a month's time (for an outside surgicenter skin case) using our hospital supplies. We are trying to draw up a contract to charge the Pathologist for using our services. Any suggestions? Thanks in advance! Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From jdmd77 <@t> hotmail.com Tue Oct 10 14:09:24 2006 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Tue Oct 10 14:09:41 2006 Subject: [Histonet] Patient consent In-Reply-To: <452BA89D0200007700002557@hcnwgwds01.hh.chs> Message-ID: Histonetters - There could be two issues at play here: (1) a perceived need for patient consent prior to consulation and (2) receiving patient's consent so that if/when the consultant ISN'T paid by the insurance company, that the patient will actually pay for the consultation. As a consultant pathologist - our practice receives cases from other physicians - both the clinician who took the biopsy/did the surgical procedure, from other pathologists AND from patient's who want "a specialist looking at their tissue." In the two former scenarios, we are consulting for the physician requesting the consultation - and that falls within HIPAA without a separate signature for patient consent. Not all referring physicians (or pathologists) understand this. I've had clinicians tell me that they can't send me clinical history without a signed consent from the patient. This, clearly, is a misunderstanding of both HIPAA and the role of the pathologist as a CONSULTING PHYSICIAN. My suspicion is that the consulting pathologist has seen that 50% of insurance companies REFUSE TO REIMBURSE for second opinions in pathology (while requiring them in clinical disciplines prior to definitive treatment - go figure)... and she may be instituting this policy within the framework of billing the patient for the consultation when the insurance doesn't pay. Julia Dahl, M.D. GI - Hepatic Pathologist Mosaic GI Research and Educational Consortium Mosaic GI-Hepatic Pathology Services >From: "Richard Cartun" >To: >Subject: [Histonet] Patient consent >Date: Tue, 10 Oct 2006 14:05:16 -0400 > >One of my colleagues wants to send a difficult case to an expert >pathologist in Toronto for a second opinion. She is requiring the >patient's consent before she accepts the case. How many of you get the >patient's consent before sending a case out for second opinion? Is this >a HIPAA requirement that I am not familiar with? Thank you. > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Add fun gadgets and colorful themes to express yourself on Windows Live Spaces http://clk.atdmt.com/MSN/go/msnnkwsp0070000001msn/direct/01/?href=http://www.get.live.com/spaces/features From LSebree <@t> uwhealth.org Tue Oct 10 14:13:48 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 10 14:13:55 2006 Subject: [Histonet] Antigenicity In-Reply-To: <452BB2D10200008C0002870D@GWIA02.HERSHEYMED.NET> Message-ID: We always cut our control slides in advance both on plain + slides and the red box control slides. Some we store at RT and others at -20 degrees C depending upon whether there are issues in loss of antigenicity of the antigen the control tissue is used for. We've determined which antigens those are through trial and error. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Tuesday, October 10, 2006 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigenicity Hello all, I have a technical question. Do any of you pre-cut your control slides in larger batches? It used to be that the control slides would lose their antigenicity if stored for long periods of time (several months) in a slide file as opposed to cutting the blocks as needed. Have the HIER techniques evolved to the point that this is no longer true? Thank you in advance. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Oct 10 14:20:05 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Oct 10 14:25:45 2006 Subject: [Histonet] Calcium oxalate procedure Message-ID: Hi everyone, If there a more contemporary procedure for testing of calcium oxalate than the Pizzolato Method? And if not, is the Pizzolato method utilizing the light-bulb (Sheehan) the most updated Pizzolato Method available? Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From TJJ <@t> Stowers-Institute.org Tue Oct 10 14:53:45 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Oct 10 14:54:10 2006 Subject: [Histonet] Re: embedding/freezing samples from glycerol Message-ID: Anna, You wrote: Hello, I recently froze embryo samples in OCT that had been sitting in 70% glycerol. When I tried to section these samples on the cryostat, the results were terrible -- the specimen basically would not be cut (appeared gooey and stuck out of the block). I wasn't able to save anything. The problem I have is that all of my samples are in glycerol, and I would eventually like to section them. :) Does anyone have any advice as to what I can do with these samples to make them section-able? They have been stained with Xgal, so I can't do anything that would weaken the stain. Thanks in advance for any advice! Best, Anna Beaudin Division of Nutritional Sciences Cornell University My advice is to paraffin process them and section them that way. There is some concern that the xylene will remove the x-gal stain, and I can assure you that unless you have the samples bathing in it for many hours, the x-gal will remain in the sample. Best wishes and good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From JWEEMS <@t> sjha.org Tue Oct 10 14:59:11 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Oct 10 14:59:31 2006 Subject: [Histonet] Patient consent Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED99@sjhaexc02.sjha.org> There was lots of confusion about that when HIPAA first took place. We confirmed with HIPAA that when case goes from doctor to doctor for patient care that this was not required. Canada is very particular, though, so I'm not sure if this is something that is required there. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, October 10, 2006 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient consent One of my colleagues wants to send a difficult case to an expert pathologist in Toronto for a second opinion. She is requiring the patient's consent before she accepts the case. How many of you get the patient's consent before sending a case out for second opinion? Is this a HIPAA requirement that I am not familiar with? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Tue Oct 10 15:04:09 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Oct 10 15:04:31 2006 Subject: [Histonet] Patient consent Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEED9A@sjhaexc02.sjha.org> Also, didn't think to say this before, but this would assure that the patient knows that a consult is occurring if there should be a bill their insurance won't cover... j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Tuesday, October 10, 2006 3:59 PM To: Richard Cartun; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Patient consent There was lots of confusion about that when HIPAA first took place. We confirmed with HIPAA that when case goes from doctor to doctor for patient care that this was not required. Canada is very particular, though, so I'm not sure if this is something that is required there. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, October 10, 2006 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient consent One of my colleagues wants to send a difficult case to an expert pathologist in Toronto for a second opinion. She is requiring the patient's consent before she accepts the case. How many of you get the patient's consent before sending a case out for second opinion? Is this a HIPAA requirement that I am not familiar with? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From TCostello <@t> gtxinc.com Tue Oct 10 15:46:07 2006 From: TCostello <@t> gtxinc.com (Terry Costello) Date: Tue Oct 10 15:46:18 2006 Subject: [Histonet] Rat Paw Protocol Message-ID: <9E8CB17DF8638A46832960D5E3DCAACDC835CC@gtxmail1.gtx.gtxinc.com> Does anyone have a protocol for histopathological processing of rat paws that they would be willing to share? It would be greatly appreciated. Thanks, Terry Terrence Costello GTx, Inc. Three North Dunlap Third Floor Van Vleet, Bldg. Memphis, TN 38163 (901) 523-9700 ext. 102 (office) (901) 523-9772 (fax) tcostello@gtxinc.com ________________________________________________________________________ ____________________________________________ This electronic message, including any attachments, is confidential and proprietary and is soley for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronis transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. From KPierce <@t> cancer-test.com Tue Oct 10 16:28:11 2006 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Tue Oct 10 16:28:19 2006 Subject: [Histonet] histotech position Message-ID: <9380B79A09A6DD43884D977256FC120204E232@fleming.mla.local> A full time (6 AM-2PM,Mon-Fri) histo tech position is available at Med Lab Assoc in Seattle, WA. MLA is a reference lab for hospitals and Dr offices in WA, AK, OR, Mt and Europe. Contact Ken Pierce at kpierce@cancer-test.com for more details. From LuckG <@t> empirehealth.org Tue Oct 10 16:40:19 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Tue Oct 10 16:43:01 2006 Subject: [Histonet] STP420 from Richard Allan. . References: <5AEC610C1CE02945BD63A395BA763EDEB1CA61@NVCIEXCH02.NVCI.org> <002f01c6ebd8$15b5b110$530114ac@w3256domain.com> Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C8F2B@IRMEXCH01.irm.inhs.org> Hellol All, Anyone else (besides Mark Tarango) out there with an STP420 tissue processor. Any and all input would be appreciated. Thanks, Greg ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Wesley Simms, MD Sent: Mon 10/9/2006 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.potas <@t> gmail.com Tue Oct 10 20:35:07 2006 From: j.potas <@t> gmail.com (Jason Potas) Date: Tue Oct 10 20:35:12 2006 Subject: [Histonet] help with luxol fast blue myline staining Message-ID: Hi everyone, im trying to make up Luxol Fast Blue to look at myelin in frozen cut spinal cord sections. Sigma makes Solvent Blue 37 and Solvent Blue 38. Which is better or the correct one to use? does anyone know what the difference is between the two? many thanks cheers jason -- ______________________________________ Jason Potas (PhD) Laboratorio de Neurobiologia Celular e Molecular Instituto de Biofisica Carlos Chagas Filho - IBCCF Universidade Federal do Rio de Janeiro CCS bloco J sala J1-029 Ilha do Fundao 21941-590 Rio de Janeiro, RJ - Brasil Tel: +5521 2280 4694 +5521 2562 6554 Email: jason@biof.ufrj.br From psicurello <@t> mcvh-vcu.edu Tue Oct 10 21:24:48 2006 From: psicurello <@t> mcvh-vcu.edu (Paula Sicurello) Date: Tue Oct 10 21:26:58 2006 Subject: [Histonet] Why ammonia water? Message-ID: Hello Histonetters, The question of the day, around here using 3% ammonia water while sectioning para We use established So if anybody out there knows why, please let me know. Paula :-) NOTE: The information contained in and confidential and protected from disclosu this message is not the intended recipient, you are h that any dissemination, distribution or copying of this comm unication is strictly prohibited. If you have received this communication the message and delet -------------------------------------- VCU Health System http://www.vcuhealth.org From sheriblair1 <@t> netzero.net Tue Oct 10 23:39:39 2006 From: sheriblair1 <@t> netzero.net (sheriblair1@netzero.net) Date: Tue Oct 10 23:40:40 2006 Subject: [Histonet] Why ammonia water? Message-ID: <20061010.214014.793.636013@webmail17.lax.untd.com> The reason I have been told was that the ammonia helps the water to get into the cells to rehydrate them more quickly than water alone. From petersen_lab_staff_health_med_uct <@t> mail.med.uct.ac.za Wed Oct 11 01:54:06 2006 From: petersen_lab_staff_health_med_uct <@t> mail.med.uct.ac.za (Morea Petersen) Date: Wed Oct 11 01:54:14 2006 Subject: [Histonet] Thioredoxin Message-ID: <1160549646.452c950ee376a@webmail.uct.ac.za> Dear Histonetters Has anyone had experience with antibodies to Human Thioredoxin, Glutaredoxin and Thioredoxin Reductase. I have antibodies produced by IMCO laboratories and would like someone who has used these to help with a suitable protocol. Thanks in advance. Morea Petersen Division of Anatomical Pathology UCT Medical School Anzio Road Observatory 7925 Cape Town South Africa Commitment leads to action. Action brings your dream closer. Marcia Wieder ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From wasielewski.reinhard.von <@t> mh-hannover.de Wed Oct 11 03:14:07 2006 From: wasielewski.reinhard.von <@t> mh-hannover.de (wasielewski.reinhard.von@mh-hannover.de) Date: Wed Oct 11 03:14:13 2006 Subject: [Histonet] Collagen immunohistochemistry in sheep Message-ID: <452CC3EF.15367.48830685@wasielewski.reinhard.von.mh-hannover.de> Hi Histonetters, does anyone have experience with immunos to differentiate collagen I vs. III (vs. VI) in sheep tissue ? The tissues are already formalin fixed and decalcified with EDTA. Any suggestions which antibodies are suitable and which protocols are best are welcome ! Thanks a lot in advance Best regards Reinhard PD Dr. med. Reinhard von Wasielewski From je2 <@t> sanger.ac.uk Wed Oct 11 04:09:32 2006 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Wed Oct 11 04:08:55 2006 Subject: [Histonet] Monoclonal antibody to Beta-galactosidase Message-ID: Hi, I need to find a monoclonal antibody to Beta-galactosidase working on FFPE mouse tissue. Could you give me some advice? Thanks Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA UK From mtyler <@t> uctgsh1.uct.ac.za Wed Oct 11 04:36:34 2006 From: mtyler <@t> uctgsh1.uct.ac.za (Tyler) Date: Wed Oct 11 04:36:43 2006 Subject: [Histonet] TFF3 antibody Message-ID: <452CBB22.BF0F76B8@uctgsh1.uct.ac.za> Morning to All Please where can I find anti -TFF3 (Trefoil Factor 3)(human) Mouse antibody.Need it for IHC in formalin fixed tissue. Thanks Marilyn From BMolinari <@t> heart.thi.tmc.edu Wed Oct 11 05:36:43 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Oct 11 05:36:52 2006 Subject: [Histonet] STP420 from Richard Allan. . In-Reply-To: <20061010153219.37983.qmail@web61219.mail.yahoo.com> Message-ID: I had some experience using the automated embedder. The two biggest disadvantages were that the pathologist has control over the way the specimen is orientated the histotech is left totally out of the equation. Once the tissue is placed into the cassette it is difficult to open again. Also Rene is correct, there is a plastic lid you have to cut thru before getting to the tissue. This presented a big problem with biopsies because the tissue would adhere to the lid and by the time you cleared away the lid you may also had cut thru the biopsy. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, October 10, 2006 10:32 AM To: Wesley Simms, MD; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . Wesley: As you perfectly know, tissue processors "liberated" the histotech from the "slavery" of hand processing but we still have to see another "liberations". SakuraFineTek now has also a piece of equipment for automated embedding that has so many restrictions regarding the initial cassetting and has so many expensive complements that I doubt "will fly" specially because the cassettes are made of a plastic that has to be sectioned ALONG with the tissue. I have not hear histotechs screaming about that yet because I don't think they have been able to sell it. On the other hand, with regards to MW assisted technology for tissue processing the only really convenient are those with the "walk away" capability and so far only the PATHOS from Milestone and the Xpress from Sakura FineTek provide it. Any other single chamber MW oven that needs the handling of the cassettes by the histotech from one station to the other is just returning to old "slavery" days. MW assisted tissue processing is something to take with several, not just one grain of salt. On the other hand if you are able to process tissue quickly just to be sitting before sectioning, makes no sense. MW assisted tissue processing without rescheduling is a waste of resources, as are those MW tissue processors with 180 blocks capabilities: to get to such a large batch you will need several hours of grossing / casseting and what are you going to do?: start the processor at 4 PM and start sectioning at 7PM through the night? Promote an even worse histotech slavery? I don't think so. This whole issue has to be analyzed cautiously; this is not a band wagon to jump into without thinking it well! Just my opinion though! Ren? J. "Wesley Simms, MD" wrote: Rene, Yes indeed, I was told last week by the nice pathologist from Miami (whose name eludes me at the moment) who Sakura brought in to teach us to use the new grossing tools that raw tissue, even if sliced at 1.5mm thick, could not be put on the machine. He quoted the 4 hour figure for breast tissue in particular. When I saw the graph of Miami's FTE staffing for HT's and pathologists by time of day (before and after Xpress), I was also puzzled as to how this would translate into any tangible benefit, either cost-wise or by improved patient care. The only result I could see was that everyone's day is going to start later and be strung out over a longer period of time. My wife, who is an attorney, summed it up best:"At least with the old processor, something was doing work overnight and being productive. Now, with the new one, nothing is." Wesley Simms ----- Original Message ----- From: Rene J Buesa To: Wesley Simms, MD ; histonet@lists.utsouthwestern.edu Sent: Tuesday, October 10, 2006 10:32 AM Subject: Re: [Histonet] STP420 from Richard Allan. . Dr. Simms: According with Morales et al. ("inventors") you can process tissue in the Xpress that has not been previously fixed, now it turns out that you cannot? That is another drawback yet, because it is also not true that you will be able to process 120 cassettes in 1 hour; their mathematics do not add up. The protocol (2 stations with MW assisted protocol) + 2 "conventional" stations (just convection heating + vacuum) takes 60 minutes to complete (15 min each). The only real advantage seems to be that you can introduce 30 cassettes every 15 minutes in the first station. This means that you will have 30 cassettes processed in 1 hour, the following 30 (to a total of 60) the next 15 minutes (=75 minutes) and so on until you get to the 120 cassettes after 105 minutes. And all of this has nothing to do, has not impacted at all, on the time required for manual work (grossing/cassetting, embedding, trimming and sectioning the blocks) that will amount to a total of 149 minuttes per every 30 cassettes, even allowing that there will be automated instruments to write cassetes, etch slides, routine stain and coverslip. The "famous" 120 cassettes / hour turn out to be a total of 105 min. processing + 160 minutes of automated ancillary tasks + 596 minutes of manual work, for a grand total of 861 min =14.4 h / 120 cassettes = 2.4 FTE with 75% time utilization on 8h shifts! I could also buy a good house with $250,000! Just a few "numbers" about the Xpress (no reagents cost included of course!) Ren? J. "Wesley Simms, MD" wrote: By "real" time, I refer to the fact that you can't load fresh tissue on the Xpress...even if it sliced at the requisite 1.5mm, several hours of fixation is required (4 hours for breast). Ergo, the "real" processing time for breast on the Xpress is five hours. In terms of cost, I have found a nice lakefront house in the Florida panhandle and a new boat that I can buy for the same price as an Xpress ($250,000). My informed sources also tell me to expect quadrupled reagent costs with the Xpress, so yes, I think your use of the term "stratosphere" is appropriate. I don't know the price of the STP240, but I cannot imagine that it is as expensive, given that it is, essentially, a turbocharged conventional processor. If the STP240's total processing time is in the 5 hour ballpark, I would certainly be interested in looking at one. Wesley W. Simms ----- Original Message ----- From: "Jasper, Thomas G." To: "Wesley Simms, MD" Cc: Sent: Monday, October 09, 2006 3:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Wesley, Could you clarify a bit? What do you mean by "real" time? And you are certain that the two are not comparable in terms of cost. Does this mean that the Tissue Tek Express is priced in the "stratosphere" or does it mean that the price is justifiable due to "real" time savings? Or is the price of the STP420 excessive because it doesn't do what the Tissue Tek Express does? Or is it a great deal because it does process quickly at a substantially lesser cost? Your statement begs for more information, because I can't tell where you stand on the issue. Thanks, Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wesley Simms, MD Sent: Monday, October 09, 2006 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STP420 from Richard Allan. . How do the "real" processing times on the STP 420 compare with the Tissue Tek Xpress? I feel certain that the two are not comparable in terms of cost. Wesley W. Simms ----- Original Message ----- From: "Tarango, Mark" To: "Henry, Charlene" ; Sent: Monday, October 09, 2006 1:56 PM Subject: RE: [Histonet] STP420 from Richard Allan. . Works great. I've never had a problem with it. I like that it is two tissue processors in one (has two chambers that can be ran at the same time). I've recently heard there are about 10 out there already. Hopefully someone else will make a comment about it. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, October 09, 2006 6:39 AM To: Tarango, Mark Subject: RE: [Histonet] STP420 from Richard Allan. . How is the instrument performing for you? Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tarango, Mark Sent: Friday, October 06, 2006 2:04 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . I have the STP420. The last time I heard I was the only one in the US who has one. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, October 06, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] STP420 from Richard Allan Has anyone had experience with this rapid tissue processor?( No vendors please) IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Oct 11 06:45:03 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Oct 11 06:45:16 2006 Subject: [Histonet] Monoclonal antibody to Beta-galactosidase Message-ID: <20061011114504.38251.qmail@web50301.mail.yahoo.com> I have been using a rabbit polyclonal from Abcam on (ab616) @ 1:800, HIER overnight at 60-65C in Biocare Reveal or BORG (don't use the BOG on tissues that are really fatty, like skin or brains - they will fall off!). The BORG gives better staining, but the Reveal is acceptable if using fatty tissues. Kim Merriam Cambridge, MA ----- Original Message ---- From: Jeanne Estabel To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 11, 2006 5:09:32 AM Subject: [Histonet] Monoclonal antibody to Beta-galactosidase Hi, I need to find a monoclonal antibody to Beta-galactosidase working on FFPE mouse tissue. Could you give me some advice? Thanks Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA UK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Oct 11 07:02:11 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Oct 11 07:03:04 2006 Subject: [Histonet] Why ammonia water? Message-ID: We don't use it. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> "sheriblair1@netzero.net" 10/11/06 12:39 AM >>> The reason I have been told was that the ammonia helps the water to get into the cells to rehydrate them more quickly than water alone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aep10 <@t> cornell.edu Wed Oct 11 07:38:19 2006 From: aep10 <@t> cornell.edu (Anna Elisse Beaudin) Date: Wed Oct 11 07:38:30 2006 Subject: [Histonet] counterstain for immunofluoroscence Message-ID: <4085.128.253.96.191.1160570299.squirrel@webmail.cornell.edu> Hello, I am processing mouse brain sections for double-immunofluorescence and need to find a suitable counterstain. I do not necessarily need the counterstain to be fluorescent, since the purpose of the counterstain is only to map the area of interest, but I want to make sure that whatever counterstain I use is not going to quench my fluoresence. I am using secondary-conjugated Alexa 488 and Streptavidin-Alexa 546. I appreciate any and all advice. Thanks in advance for your help! Best, Anna Beaudin Division of Nutritional Sciences Cornell University From rjbuesa <@t> yahoo.com Wed Oct 11 07:39:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 11 07:39:11 2006 Subject: [Histonet] Why ammonia water? In-Reply-To: Message-ID: <20061011123904.69065.qmail@web61221.mail.yahoo.com> Paula: Ammonia water is an alkaline solution and, as all alcaline solutions (soap included), it has softening qualities (as when you feel your hands softer after washing your hands with soap). Since the solution can also "penetrate" and "soak" the processed tissue, it makes it softer and easier to section, specially decals. Ren? J. Paula Sicurello wrote: Hello Histonetters, The question of the day, around here = anyway, What is the history of using 3% ammonia water while sectioning para= ffin? We use= it for small biopsies and skins but we don't know why and who established = that protocol anyway. So if anybody out there knows why, please let me know. Paula :-) NOTE: The information contained in = this message may be privileged and confidential and protected from disclosu= re. If the reader of this message is not the intended recipient, you are h= ereby notified that any dissemination, distribution or copying of this comm unication is strictly prohibited. If you have received this communication = in error, please notify us immediately by replying to the message and delet= ing it from your computer. -------------------------------------- VCU Health System http://www.vcuhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From lucy.anderson <@t> ucl.ac.uk Wed Oct 11 07:57:34 2006 From: lucy.anderson <@t> ucl.ac.uk (L. Anderson) Date: Wed Oct 11 07:57:48 2006 Subject: [Histonet] SMI neurofilaments in mouse Message-ID: <001201c6ed34$d22a55f0$94f82880@DP370LUCY> Dear All, I am looking at carrying out some SMI-32 staining in mouse brains, specifically auditory thalamus and cortex. I know SMI-32 has been used successfully in these areas in a number of species including cats and ferrets, but I have received mixed reports as to its usefulness in rodents. Can anyone offer any advice on the efficacy of SMI staining in mice, and suitable protocols? Many regards, Lucy **************************************** Lucy Anderson Research Fellow UCL Centre of Auditory Research 332 Gray's Inn Road London WC1X 8EE From tp2 <@t> medicine.wisc.edu Wed Oct 11 08:49:52 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Wed Oct 11 08:50:27 2006 Subject: [Histonet] counterstain for immunofluoroscence Message-ID: <452CB031020000DF00001F20@gwmail.medicine.wisc.edu> If you wanted a flourescent nuclear counterstain you could use DAPI or Hoechst. There are mounting mediums that already have DAPI in them like SlowFade Gold with DAPI. Tom Pier >>> "Anna Elisse Beaudin" 10/11/06 7:38 AM >>> Hello, I am processing mouse brain sections for double-immunofluorescence and need to find a suitable counterstain. I do not necessarily need the counterstain to be fluorescent, since the purpose of the counterstain is only to map the area of interest, but I want to make sure that whatever counterstain I use is not going to quench my fluoresence. I am using secondary-conjugated Alexa 488 and Streptavidin-Alexa 546. I appreciate any and all advice. Thanks in advance for your help! Best, Anna Beaudin Division of Nutritional Sciences Cornell University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Wed Oct 11 09:06:56 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Wed Oct 11 09:07:06 2006 Subject: [Histonet] freezing kidney bx Message-ID: <9B4A77DF11463E4FB723D484214AE9BC01CD110E@KALEXMB02.KaleidaHealth.org> When freezing kidney biopsies for the purpose of direct immunofluourescence what is the recommendation....snap freezing in liquid nitrogen or freezing in a cryostat? Thanks Annette Featherstone HT/MLT ASCP, MT (HEW) Supervisor Anatomic Pathology Kaleida Health, Buffalo General Hospital 100 High Street Buffalo NY 14203 716-859-2625 FAX: 716-859-1853 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From anh2006 <@t> med.cornell.edu Wed Oct 11 08:23:11 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Oct 11 09:08:29 2006 Subject: [Histonet] counterstain for immunofluoroscence In-Reply-To: <4085.128.253.96.191.1160570299.squirrel@webmail.cornell.edu> References: <4085.128.253.96.191.1160570299.squirrel@webmail.cornell.edu> Message-ID: DAPI, Hoechst 33342 are excellent for this purpose assuming you have the appropriate filter sets on your scope for that wavelength. Alternatively if you are doing confocal with a far red laser you can use Draq-5 or Topro-3. >Hello, > > I am processing mouse brain sections for double-immunofluorescence and >need to find a suitable counterstain. I do not necessarily need the >counterstain to be fluorescent, since the purpose of the counterstain >is only to map the area of interest, but I want to make sure that >whatever counterstain I use is not going to quench my fluoresence. I am >using secondary-conjugated Alexa 488 and Streptavidin-Alexa 546. I >appreciate any and all advice. Thanks in advance for your help! > >Best, >Anna Beaudin >Division of Nutritional Sciences >Cornell University -- From rjbuesa <@t> yahoo.com Wed Oct 11 09:13:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 11 09:13:45 2006 Subject: [Histonet] Are we 49,000 strong? Message-ID: <20061011141335.18335.qmail@web61223.mail.yahoo.com> Dear Histonet subscriber: A week ago I asked your opinion about being 49,000 strong. Following is a summary of 17 answers that included some quatification; the other respondents just said: "I don't know!" or "I think we will never know!". A- We are LESS than 49k (1 answer) because many hospitals in Texas send their cases to an independent lab. Extrapolating the Texas case to the whole nation, we could be 39,000 B- Probably WE ARE 49k (4 answers) although all accepted 49k as a possible figure, they all said it was a "big guess" and that we "will never know". C- We are MORE than 49k ( 12 answers). They thnk we are MORE than 49k because I did NOT include in my initial calculations many places doing histology work like: government institutions, pharmaceutical companies, private contract labs, large reference labs, biotechnology companies, temporary employment agencies, microscopy facilities, museums, zoological gardens, aquaria and marine research labs and military labs that have a histologist job category and a med. tech category trained to do histology work. Even the fact that Mohs techs (that do FS and H&E on a regular basis and that consider themselves as histotechs) were not included when they should be. Those colleagues offered levels of more than 50k, probably 58k, around 60k or between 60k and 70k Although the 49k initial number came from some actual figures (histology labs within known numbers of hospitals and average numbers of HT per lab) it is a "guestimate", in the same way that "considering" we are more or less are also speculative opinions, "gut feelings" without supporting "hard evidence". Nevertheless, there are so many places nationwide that do some sort of histology work, Histonet has so many subscribers seeking answers to histology problems that I do not think it would be too farfetched to say that we may be between 39,000 and 58,000 (the later being the average from 50k, 58k, 60k and 65k pointed out as possible maximum levels). Are we 49,000 strong? We may be between 39,000 and 58,000 (average of 49,000) strong! Ren? J. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From kewleys <@t> health.missouri.edu Wed Oct 11 10:13:16 2006 From: kewleys <@t> health.missouri.edu (Kewley, Sharyl F.) Date: Wed Oct 11 10:13:25 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 21 References: Message-ID: <93D0418A67D27C47BD90CF3A0DCC39F524A275@UM-XMAIL04.um.umsystem.edu> Hi Jason, I have checked in Conn's Biological Stains and for the Luxol Fast Blue dye it states that the C.I. is Solvent Blue 38, so I believe that is what you will want to use. You stated that Sigma made a 37 and a 38, you will need to order Solvent Blue 38 from Sigma. I am not sure what the difference is between Solvent Blue 37 and 38, but, in the Sigma catalog if you will notice the 37 says only practical grade and the 38 says Luxol Fast Blue MBSN and then practical grade. So to me that means that only the 38 pertains to the Luxol Fast Blue staining. If you have access to a Conn's Biological Stains book it will give you lots of information on all different staining dyes. Hope this helps, Sharyl Kewley, HT (ASCP) Columbia Regional Hospital 404 Keene St. Columbia, MO 65201 ________________________________ :------------------------------ Message: 25 Date: Tue, 10 Oct 2006 22:35:07 -0300 From: "Jason Potas" Subject: [Histonet] help with luxol fast blue myline staining To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi everyone, im trying to make up Luxol Fast Blue to look at myelin in frozen cut spinal cord sections. Sigma makes Solvent Blue 37 and Solvent Blue 38. Which is better or the correct one to use? does anyone know what the difference is between the two? many thanks cheers jason -- ______________________________________ Jason Potas (PhD) Laboratorio de Neurobiologia Celular e Molecular Instituto de Biofisica Carlos Chagas Filho - IBCCF Universidade Federal do Rio de Janeiro CCS bloco J sala J1-029 Ilha do Fundao 21941-590 Rio de Janeiro, RJ - Brasil Tel: +5521 2280 4694 +5521 2562 6554 Email: jason@biof.ufrj.br ------------------------------ From TillRenee <@t> uams.edu Wed Oct 11 10:19:42 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Wed Oct 11 10:20:17 2006 Subject: [Histonet] what are those cells? Message-ID: <11F927674DEBDC43B960809A7403C5D2022D5CFE@MAILPED.ad.uams.edu> Hello. Are there any colon experts out there who could maybe identify some cells for me if I send you a picture? I have done a PTEN immuno on proximal and distal rat colon and am getting strong staining in some cells in the lamnia propria of the distal colon, but not as much in the proximal. We are intrigued by this, but even the PhD who I am doing to work for is not sure what the cells are. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jqb7 <@t> cdc.gov Wed Oct 11 11:03:49 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Oct 11 11:20:49 2006 Subject: [Histonet] Xpress and AutoTEC comments Message-ID: All, I am not here to sing the praises of Sakura but I do want to make a few comments. We have just received our AutoTEC but prior to its purchase I did get a sampling of the Paraform cassettes and tried them. The mesh-like structure is not plastic and the main surface piece is cut away with trimming leaving an edge that does not affect ribboning. I had no trouble achieving good sections with them. As far as the Xpress goes, it suits our purpose very well. We are not a hospital so I cannot comment on how it works in that environment but we are quite satisfied. An aside regarding a comment I read that someone with a source knew they were going to quadruple the price on the kits....well, I spoke with my rep. and she said absolutely not. "We are not in the business of making our customers angry!' I tend to believe her. Just my two cents worth...... Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, October 10, 2006 11:57 AM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . Karen: That is exactly what I was referring to. There is no way that adding something hard enough to survive the process that is able to keep pushed down the tissue is going to cut "just as if it did not exist". On the other hand, if you are going to use the Xpress along with this automated embedding instrument, you have to change the cassettes holders and reduce them to just 20 per run which means cutting the throughput Xpress capability by 34% (to just 80 cassettes / 105 minutes). Ren? J. "Heckford, Karen - SMMC-SF" wrote: I have actually tried the new cassette that you afe suppose to cut the plastic with the tissue. It was so absolutely horrible I had to laugh. What will they think of next? Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From liz <@t> premierlab.com Wed Oct 11 11:27:38 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Oct 11 11:23:26 2006 Subject: [Histonet] Collagen immunohistochemistry in sheep In-Reply-To: <452CC3EF.15367.48830685@wasielewski.reinhard.von.mh-hannover.de> Message-ID: <001e01c6ed52$2c753da0$0300a8c0@domain.Premier> Reinhard I have not ran collagen immunostains specifically on sheep, but we have run them on mouse, rat, human and goat bone sections that have been formic acid decaled and the antibodies that are listed below work for those species so I suspect that they will also work for sheep. Collagen I - Rabbit Polyclonal - Biogenesis Collagen II - Rabbit Polyclonal - MD biosciences Collagen III - Rabbit Polyclonal - Biogenesis Hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wasielewski.reinhard.von@mh-hannover.de Sent: Wednesday, October 11, 2006 2:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Collagen immunohistochemistry in sheep Hi Histonetters, does anyone have experience with immunos to differentiate collagen I vs. III (vs. VI) in sheep tissue ? The tissues are already formalin fixed and decalcified with EDTA. Any suggestions which antibodies are suitable and which protocols are best are welcome ! Thanks a lot in advance Best regards Reinhard PD Dr. med. Reinhard von Wasielewski _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1796 (20061010) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From liz <@t> premierlab.com Wed Oct 11 11:36:07 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Oct 11 11:31:54 2006 Subject: [Histonet] Rat Paw Protocol In-Reply-To: <9E8CB17DF8638A46832960D5E3DCAACDC835CC@gtxmail1.gtx.gtxinc.com> Message-ID: <002401c6ed53$59fba3d0$0300a8c0@domain.Premier> Terry Are you processing the paws or digits or just the ankles? I can help with some info, if I have some more specifics. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terry Costello Sent: Tuesday, October 10, 2006 2:46 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat Paw Protocol Does anyone have a protocol for histopathological processing of rat paws that they would be willing to share? It would be greatly appreciated. Thanks, Terry Terrence Costello GTx, Inc. Three North Dunlap Third Floor Van Vleet, Bldg. Memphis, TN 38163 (901) 523-9700 ext. 102 (office) (901) 523-9772 (fax) tcostello@gtxinc.com ________________________________________________________________________ ____________________________________________ This electronic message, including any attachments, is confidential and proprietary and is soley for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronis transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1796 (20061010) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From amylee779 <@t> yahoo.com Wed Oct 11 12:43:40 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Oct 11 12:43:45 2006 Subject: [Histonet] How do you discard formalin that has been used to fix animal tissue? Message-ID: <20061011174341.11429.qmail@web58304.mail.re3.yahoo.com> Hello, I used to leave them in the bottle and put into red biohazard bag. Now I am rethinking about this. So how do you do this? I appreciate any input. Amy --------------------------------- Get your email and more, right on the new Yahoo.com From maa8 <@t> cornell.edu Wed Oct 11 12:57:46 2006 From: maa8 <@t> cornell.edu (Mary Ascenzi) Date: Wed Oct 11 12:57:09 2006 Subject: [Histonet] C4d Antibody Message-ID: <5.2.1.1.2.20061011135735.00afbf98@postoffice6.mail.cornell.edu> American Research Products has an excellant C4d antibody. This is a link to their page. http://www.arp1.com/search.php?keyword=c4d&category=&Submit=Search&what=search Here is a link to pics of slides I have stained using their C4d: http://www.immunoportal.com/modules.php?set_albumName=album01&op=modload&name=Gallery&file=index&include=view_album.php&page=3 Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Forgot to mention the C4d was on formalin fixed, paraffin embedded tissue with citrate buffer HIER and using Dako's Envision for detection. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com ______________________ From MSHERWOOD <@t> PARTNERS.ORG Wed Oct 11 13:23:27 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Oct 11 13:23:32 2006 Subject: [Histonet] How do you discard formalin that has been used to fixanimal tissue? In-Reply-To: <20061011174341.11429.qmail@web58304.mail.re3.yahoo.com> Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30C8E@PHSXMB1.partners.org> We collect the formalin for hazardous waste pick-up. We've never put it into a red biohazard bag. Once the vials have been emptied, we put cap back on and throw in regular trash. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy Lee Sent: Wednesday, October 11, 2006 1:44 PM To: histonet Subject: [Histonet] How do you discard formalin that has been used to fixanimal tissue? Hello, I used to leave them in the bottle and put into red biohazard bag. Now I am rethinking about this. So how do you do this? I appreciate any input. Amy --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Oct 11 14:27:39 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Oct 11 14:27:50 2006 Subject: [Histonet] New Mexico Society for Histology Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB2A6@nmdamailsvr.nmda.ad.nmsu.edu> The NMSH Annual Meeting - the first one in over four years - is a "go"! We will have over 35 techs, almost twenty vendors present (in person or by way of material on display) and a great program for this year's meeting on October 21st. If anyone out there has been on another planet for the last several months and, hearing about our meeting, would like to come - get in touch with me pronto! I can arrange for one more tuna sandwich on that Saturday if you get your word in by the end of this week. I'd like to thank all the techs that responded and registered and the many terrific vendors that have given of their time and resources to help us rejuvenate our Society. To get on the mailing list for the NMSH newsletter, contact me at nmhisto@aol.com. I've got to go take a nap now... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From wsimms <@t> mcintoshclinic.com Wed Oct 11 16:18:13 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Wed Oct 11 16:18:26 2006 Subject: [Histonet] Xpress and AutoTEC comments References: Message-ID: <003501c6ed7a$c38368c0$530114ac@w3256domain.com> The reference re. quadrupled reagent costs was regarding reagent cost of the Xpress vs. reagent cost of conventional processing (4:1). As there are only about 48 Xpress'es in the US, I seriously doubt they will be increasing the cost of their kits...yet. Wesley W. Simms ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Wednesday, October 11, 2006 12:03 PM Subject: [Histonet] Xpress and AutoTEC comments All, I am not here to sing the praises of Sakura but I do want to make a few comments. We have just received our AutoTEC but prior to its purchase I did get a sampling of the Paraform cassettes and tried them. The mesh-like structure is not plastic and the main surface piece is cut away with trimming leaving an edge that does not affect ribboning. I had no trouble achieving good sections with them. As far as the Xpress goes, it suits our purpose very well. We are not a hospital so I cannot comment on how it works in that environment but we are quite satisfied. An aside regarding a comment I read that someone with a source knew they were going to quadruple the price on the kits....well, I spoke with my rep. and she said absolutely not. "We are not in the business of making our customers angry!' I tend to believe her. Just my two cents worth...... Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, October 10, 2006 11:57 AM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] STP420 from Richard Allan. . Karen: That is exactly what I was referring to. There is no way that adding something hard enough to survive the process that is able to keep pushed down the tissue is going to cut "just as if it did not exist". On the other hand, if you are going to use the Xpress along with this automated embedding instrument, you have to change the cassettes holders and reduce them to just 20 per run which means cutting the throughput Xpress capability by 34% (to just 80 cassettes / 105 minutes). Ren? J. "Heckford, Karen - SMMC-SF" wrote: I have actually tried the new cassette that you afe suppose to cut the plastic with the tissue. It was so absolutely horrible I had to laugh. What will they think of next? Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vann_schaffner <@t> yahoo.com Wed Oct 11 18:00:22 2006 From: vann_schaffner <@t> yahoo.com (Vann Schaffner) Date: Wed Oct 11 18:00:26 2006 Subject: [Histonet] Lurking Pathologist seeks Histotech aquintances.... Message-ID: <20061011230022.35736.qmail@web38903.mail.mud.yahoo.com> Hi all, I am a pathologist that lurks on your prolific email list. I am trying to find histotechs that I know and have lost contact with, especially if interested in a histotech position in Spokane Washington. Nice Place! Thanks! Vann Schaffner, MD Pathology Services PS Deaconess Medical Center Spokane Washington vann_schaffner@yahoo.com From tbraud <@t> holyredeemer.com Thu Oct 12 08:33:15 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Oct 12 08:34:47 2006 Subject: [Histonet] free blades Message-ID: Cleaning house. I have 4 boxes of Accu-Edge, High Profile Blades, #4685, 2 unopened, and 2 out of the box. Just send me a name and address and they're yours. Free shipping within the continental US. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory Holy Redeemer Hospital (215) 938-3689 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From msviapiano <@t> yahoo.com Thu Oct 12 08:49:42 2006 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Thu Oct 12 08:49:50 2006 Subject: [Histonet] Antibodies from Abnova Message-ID: <20061012134942.16263.qmail@web54507.mail.yahoo.com> Hi all! Does anyone have experience with antibodies from this company?. Most of what they sell seems to be non-purified mouse antisera. Thanks! Mariano Mariano S. Viapiano, PhD Center for Molecular Neurobiology Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mcauliff <@t> umdnj.edu Thu Oct 12 09:00:41 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Oct 12 09:01:59 2006 Subject: [Histonet] buffer for mounting floating brain sections In-Reply-To: <50479E31-CAC2-41A0-AEB0-A907BE636E34@bidmc.harvard.edu> References: <50479E31-CAC2-41A0-AEB0-A907BE636E34@bidmc.harvard.edu> Message-ID: <452E4A89.8020105@umdnj.edu> Hi Caroline: PBS IS phosphate buffer with saline. I have had the same problem you are experiencing and I used a dilute phos. buffer. 0.05 M without saline to mount the sections. As soon as the sections are adhered I rinsed gently with distilled water and blot off the excess. Time consuming but it worked. Geoff Caroline Bass wrote: > Hey guys, > > I know this is a question that has probably popped up before, but I > searched the archives and didn't find exactly what I need. > > I want to mount floating mouse brain sections (40 micron, NBF fixed, > sucrose protected) onto superfrost slides. I will immediately > coverslip after drying, they will not be processed further. What is > the best buffer to float the sections in when mounting onto the > slides. PBS seems to leave a lot of crystals, I have heard that 0.1M > phosphate buffer is better. Distilled water results in a lot of > curls. Since I am not going to process the slides I don't have to > worry about them falling off later (which I heard is a problem with > charged slides and PBS). > > If there is a preferred buffer could you also include the recipe. > > Thanks, > > Caroline > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From tbraud <@t> holyredeemer.com Thu Oct 12 09:07:15 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Oct 12 09:08:59 2006 Subject: [Histonet] Blades are gone Message-ID: Thanks to all who responded!!! Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory Holy Redeemer Hospital (215) 938-3689 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From ganesett <@t> fmed.edu.uy Thu Oct 12 13:31:51 2006 From: ganesett <@t> fmed.edu.uy (Gabriel Anesetti) Date: Thu Oct 12 12:20:29 2006 Subject: [Histonet] Neu N antibody Message-ID: <452E8A17.6080209@fmed.edu.uy> Hello All, Does anyone know a provider of an antibody called "NeuN" not raised in mouse? Thank-you in Advance, Gabriel From bakerj <@t> umich.edu Thu Oct 12 12:33:02 2006 From: bakerj <@t> umich.edu (John Baker) Date: Thu Oct 12 12:35:15 2006 Subject: [Histonet] cryosectioning rat bone Message-ID: Hi All, Does anyone out there in histoland get good cryo-sections of rat femur or tibia? I get great 5-7 micron sections of mouse bone using the CryoJane Tape System. Whether longitudinal or cross the cortical bone will not laminate and comes off with the window. I certainly don't get sections at all using the anti-roll plate. I have a Hacker-Bright OTF cryostat and use t-c blades. Any suggestions are greatly appreciated. Thanks in advance. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From mobine <@t> MIT.EDU Thu Oct 12 13:01:52 2006 From: mobine <@t> MIT.EDU (Hector Mobine) Date: Thu Oct 12 13:04:31 2006 Subject: [Histonet] Imaging Histological Specimens In-Reply-To: Message-ID: <200610121803.k9CI3tgv004384@outgoing.mit.edu> Hi Netters, I've been put in charge of finding a lens for our Nikon D70 that can be used to image histological specimens (fixed rat hearts and organs mainly) after paraffin-embedding and for imaging during surgery. I was told a 60mm Nikon Macro lens would work well, but am concerned that it may need to be placed too close to the subject during surgery violating the sterile requirements. Anyone have experience with lenses that could help me out? Thank you in advance. Hector Mobine From SEGoebel <@t> mdanderson.org Thu Oct 12 13:13:57 2006 From: SEGoebel <@t> mdanderson.org (SEGoebel@mdanderson.org) Date: Thu Oct 12 13:14:21 2006 Subject: [Histonet] (no subject) Message-ID: We are looking for a xylene substitute that works well on bone, does anyone have any information on a good one to use. We have tried Clearify in the past and our pathologists didn't like the cellular staining for this one. Thanks Sarah Elizabeth Goebel, B.A., HT(ASCP) M.D. Anderson Cancer Center Michale E. Keeling Center for Comparative Medicine and Research Bastrop, Texas 78602 From rjbuesa <@t> yahoo.com Thu Oct 12 13:23:58 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 12 13:24:03 2006 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <20061012182358.566.qmail@web61221.mail.yahoo.com> Try just plain naphtha (also known as "white gasoline"). Cheap, works well and is 5 times less toxic than xylene. Ren? J. SEGoebel@mdanderson.org wrote: We are looking for a xylene substitute that works well on bone, does anyone have any information on a good one to use. We have tried Clearify in the past and our pathologists didn't like the cellular staining for this one. Thanks Sarah Elizabeth Goebel, B.A., HT(ASCP) M.D. Anderson Cancer Center Michale E. Keeling Center for Comparative Medicine and Research Bastrop, Texas 78602 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From mcauliff <@t> umdnj.edu Thu Oct 12 13:29:28 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Oct 12 13:31:31 2006 Subject: [Histonet] Imaging Histological Specimens In-Reply-To: <200610121803.k9CI3tgv004384@outgoing.mit.edu> References: <200610121803.k9CI3tgv004384@outgoing.mit.edu> Message-ID: <452E8988.3050005@umdnj.edu> Hi Hector: For photography during surgery a much longer lens is needed to provide longer working distance and avoid the problems you mentioned. For the F series Nikons (F4, F5) the 180 mm lens was often used. I don't know if that lens will fit the D series cameras, call NikonUSA and ask them. Perhaps they make a longer macro lens for the D series. If you want more than life size magnification of rat hearts, etc. you will need to extend the lens with a bellows or extension tubes. Again, call NikonUSA and tell them what you want to do. Geoff Hector Mobine wrote: >Hi Netters, > I've been put in charge of finding a lens for our Nikon D70 that can >be used to image histological specimens (fixed rat hearts and organs mainly) >after paraffin-embedding and for imaging during surgery. I was told a 60mm >Nikon Macro lens would work well, but am concerned that it may need to be >placed too close to the subject during surgery violating the sterile >requirements. Anyone have experience with lenses that could help me out? > >Thank you in advance. > >Hector Mobine > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From mward <@t> wfubmc.edu Thu Oct 12 15:02:38 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Oct 12 15:02:49 2006 Subject: [Histonet] Neurofilament antibody Message-ID: <61135F0455D33347B5AAE209B903A30416CA675B@EXCHVS2.medctr.ad.wfubmc.edu> I just received a notice from Dako that they are discontinuing their neurofilament antibody. What other vendor are labs switching to? Thanks in advance for the help. Martha Ward Wake Forest University Baptist Medical Center From DGNajarian <@t> comcast.net Thu Oct 12 15:27:27 2006 From: DGNajarian <@t> comcast.net (Dennis Najarian) Date: Thu Oct 12 15:27:38 2006 Subject: [Histonet] Neurofilament antibody In-Reply-To: <61135F0455D33347B5AAE209B903A30416CA675B@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: Upstate/Chemicon, part of Millipore Corporation has several: http://www.upstate.com/browse/search.asp?q=neurofilament&dl=UPSTATE_US -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Thursday, October 12, 2006 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neurofilament antibody I just received a notice from Dako that they are discontinuing their neurofilament antibody. What other vendor are labs switching to? Thanks in advance for the help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.wei <@t> biogenex.com Thu Oct 12 16:08:24 2006 From: m.wei <@t> biogenex.com (May Wei) Date: Thu Oct 12 16:08:48 2006 Subject: [Histonet] Neurofilament antibody Message-ID: <37DC9F93CF7F864182D0463EF93D571B24DD8D@ISLETON2.california.biogenex.com> BioGenex's Neurofilament clone NE-14. AM073-5M RTU 6ml, MU073-UC concentrated 1ml. May Wei, M. Med., MBA BioGenex Laboratories www.biogenex.com Tel: 800 421 4149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Thursday, October 12, 2006 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neurofilament antibody I just received a notice from Dako that they are discontinuing their neurofilament antibody. What other vendor are labs switching to? Thanks in advance for the help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Oct 12 17:47:46 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Oct 12 17:48:18 2006 Subject: [Histonet] freezing kidney bx In-Reply-To: <9B4A77DF11463E4FB723D484214AE9BC01CD110E@KALEXMB02.KaleidaHealth.org> References: <9B4A77DF11463E4FB723D484214AE9BC01CD110E@KALEXMB02.KaleidaHealth.org> Message-ID: <452E8DD20200007700002594@hcnwgwds01.hh.chs> We freeze ours in the cryostat. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Featherstone, Annette" 10/11/06 10:06 AM >>> When freezing kidney biopsies for the purpose of direct immunofluourescence what is the recommendation....snap freezing in liquid nitrogen or freezing in a cryostat? Thanks Annette Featherstone HT/MLT ASCP, MT (HEW) Supervisor Anatomic Pathology Kaleida Health, Buffalo General Hospital 100 High Street Buffalo NY 14203 716-859-2625 FAX: 716-859-1853 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DeBrosse_Beatrice <@t> Allergan.com Thu Oct 12 17:56:39 2006 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Thu Oct 12 17:57:12 2006 Subject: [Histonet] freezing kidney bx Message-ID: We used to snap freeze kidney bx's for direct IF in liquid nitrogen. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, October 12, 2006 3:48 PM To: Annette Featherstone; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] freezing kidney bx We freeze ours in the cryostat. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Featherstone, Annette" 10/11/06 10:06 AM >>> When freezing kidney biopsies for the purpose of direct immunofluourescence what is the recommendation....snap freezing in liquid nitrogen or freezing in a cryostat? Thanks Annette Featherstone HT/MLT ASCP, MT (HEW) Supervisor Anatomic Pathology Kaleida Health, Buffalo General Hospital 100 High Street Buffalo NY 14203 716-859-2625 FAX: 716-859-1853 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Spirowg <@t> aol.com Thu Oct 12 19:03:21 2006 From: Spirowg <@t> aol.com (Spirowg@aol.com) Date: Thu Oct 12 19:03:31 2006 Subject: [Histonet] Re: Histonet Digest, Vol 35, Issue 23 Message-ID: Hello I live in the Chicago area and I am looking for a hospital that will train me. I would like to do the online distant certificate program through the U of Indiana, but I need a lab that will let me use the facilities to train me. Thanks, Spiro From katri <@t> cogeco.ca Thu Oct 12 21:22:32 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Thu Oct 12 21:22:33 2006 Subject: [Histonet] freezing kidney bx References: <9B4A77DF11463E4FB723D484214AE9BC01CD110E@KALEXMB02.KaleidaHealth.org> Message-ID: <007501c6ee6e$70d701f0$6a9a9618@Katri> We freeze in the cryostat without any problems. Katri Katri Tuomala St.Joseph's Healthcare Hamilton, Ontario, Canada ----- Original Message ----- From: "Featherstone, Annette" To: Sent: Wednesday, October 11, 2006 10:06 AM Subject: [Histonet] freezing kidney bx When freezing kidney biopsies for the purpose of direct immunofluourescence what is the recommendation....snap freezing in liquid nitrogen or freezing in a cryostat? Thanks Annette Featherstone HT/MLT ASCP, MT (HEW) Supervisor Anatomic Pathology Kaleida Health, Buffalo General Hospital 100 High Street Buffalo NY 14203 716-859-2625 FAX: 716-859-1853 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Wed Oct 11 16:05:25 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Oct 13 01:52:56 2006 Subject: [Histonet] 5 New Openings for Temporary and Permanent Histology Technicians {Histo Techs} (10/11/06) Message-ID: Hi - Fellow-Histonetters - Are you looking for a change ? Below is the updated list of both temp and perm Histology jobs, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech jobs are permanent full-time unless indicated otherwise Also, if your not interested in any of these great opportunities and prefer a different location, please call me and let me know where and I can find it for you. MY HOTTEST JOB RIGHT NOW IS A TEMPORARY HISTOLOGY ASSIGNMENT IN PENNSYLVANIA, GREAT LOCATION, TOP DOLLAR, CALL TODAY- NEEDS TO BE FILLED BY OCTOBER 16TH 2006 Permanent Histology Jobs: ------------------------------------------------------ 1. Southwest Texas- Bench Histotech- perm - NEW 2. California (Bay Area) - Bench Histotech- perm -NEW 3. Texas (Dallas Area)- Bench Histotech- perm- NEW 4. Tennessee (Nashville Suburb) - Bench Histotech- perm- Hard Tissue (Bones) -NEW 5. Virginia (Northern) - Bench Histotech - perm- NEW 6. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 7. Northern New Jersey - Histotech - perm 8. Boston Mass - Histotech - one Senior Histotech, One not so Senior Histotech 9. Boston Mass - Histotech - Histotech -perm 10. Massachusetts (North of Boston) - perm - Bench Histotech 11. Central Florida -perm- Histotech 12. Southeast Florida - Treasure Coast - perm - Histotech 13. Southeast Florida - perm - Histotech 14. South Florida - perm - Bench Histotech 15. Florida, West Coast - both temp & perm openings- Bench Histotech 16. New Hampshire perm openings - Bench Histotech 17. New York ( Syracuse area) - Bench Histotech- perm 18. Central Florida - Bench Histotech- perm -----------------------------------end perm jobs ---------------------------------------------------- Temporary Assignments ------------------------------- 1. Massachusetts (Boston area) - Histo Tech minimum of 13 weeks (2 people) 2. Pennsylvania - Histotech- 6 months -HOT-HOT-HOT 3. New Mexico - Histotech- 10-12 weeks 4. Florida (South East)(Treasure Coast)- minimum of 4 weeks possibly longer -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From arvind <@t> nbrc.res.in Fri Oct 13 02:39:21 2006 From: arvind <@t> nbrc.res.in (Arvind Pundir) Date: Fri Oct 13 02:34:17 2006 Subject: [Histonet] querry References: Message-ID: Dear all i would like to have a favour from all members who can help , i am standardising the protocol for slice culture of bird brain , can anyone brief the protocol , i will be doing IHC on them later that means i need to section them further at around 10-50um , any suggestion are humbly requested Arvind Singh Pundir National Brain Research Centre Manesar , Gurgaon , Haryana INDIA 122050 From cpomajzl <@t> cpllabs.com Fri Oct 13 06:51:13 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Oct 13 06:51:29 2006 Subject: [Histonet] Full-Time HT Positions in Austin, TX Message-ID: <002f01c6eebd$e25e7a70$26fca8c0@cpllabs.com> Histonetters: Clinical Pathology Laboratories in Austin Texas is looking for full-time histotechs. Come down to the beautiful Hill Country of Central Texas with beautiful lakes and serene landscapes nestled near our state capital. HT certification preferred but not required. We are the largest private reference laboratory in the United States. We offer very competitive pay, health benefits, and a company profit sharing program. All interested applicants please call me or respond to this e-mail. You may also contact our Human Resources department at: 512-873-1607 (office) 512-873-5098 (fax) Sincerely, Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. From srishan <@t> mail.holyname.org Fri Oct 13 10:40:44 2006 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Fri Oct 13 10:41:13 2006 Subject: [Histonet] cap question regarding the microwave Message-ID: Hi, I would like to get some feed back on this cap question ANP. 28290 ARE MICROWAVE DEVICES PERIODICALLY MONITORED FOR TEMPERATURE REPRODUCIBILITY? THANKS NIRMALA From TaylorJA <@t> missouri.edu Fri Oct 13 11:00:19 2006 From: TaylorJA <@t> missouri.edu (Julia Taylor) Date: Fri Oct 13 11:00:31 2006 Subject: [Histonet] Damon/IEC minotome Message-ID: <046786a5b67bb5c20c70561e9facd742@missouri.edu> Hello. I was wondering if anybody has experience with the IEC 3398 minotome. Our multiple-user cryostat is down for repair, and we've been loaned one of these as a substitute. It has been sitting untouched for several years, and has no blades or manual. We're trying to determine a) what kind of blades to use and b) where to obtain them. Disposable blades would be preferable (based on both cost and the mulit-user issue). If anyone can tell me either of these things (and/or even give us practical hints on its use, since it's not at all like the departed Leica Frigocut cryostat)) I'd very much appreciate it. Thanks -JT. From rjbuesa <@t> yahoo.com Fri Oct 13 11:21:15 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 13 11:21:26 2006 Subject: [Histonet] cap question regarding the microwave In-Reply-To: Message-ID: <20061013162115.9800.qmail@web61213.mail.yahoo.com> Nirmala: As you know, QA records require procedural consistency. Some laboratory designed microwave ovens (MWO) can monitor temperature and, as a matter of fact, the program sets the desired temp. and the time, "leaving" the MWO to set the energy level to reach both requirements. Those MWO usually can print a protocol log in which temp. is recorded. If the MWO is not lab designed, or if it does not have the recording capability, the temp has to be determined with a probe. The whole idea is to make sure that the tissues are subjected only to a pre-determined temp, in the same way that each day the temp. of the retort on any regular tissue processor is recorded. The CAP requirement is that there should be a record, either automatic or logged, with the temp. the tissues have been exposed during the processing protocol. Ren? J. srishan@mail.holyname.org wrote: Hi, I would like to get some feed back on this cap question ANP. 28290 ARE MICROWAVE DEVICES PERIODICALLY MONITORED FOR TEMPERATURE REPRODUCIBILITY? THANKS NIRMALA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From mward <@t> wfubmc.edu Fri Oct 13 11:39:46 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Oct 13 11:39:53 2006 Subject: [Histonet] Neurofilament antibody Message-ID: <61135F0455D33347B5AAE209B903A30416CEC634@EXCHVS2.medctr.ad.wfubmc.edu> Thanks to everyone who responded to my request. I appreciate the information. Martha Ward From JWEEMS <@t> sjha.org Fri Oct 13 11:40:07 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 13 11:40:32 2006 Subject: [Histonet] Workload comparison Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE54@sjhaexc02.sjha.org> We have consultant's on site! Would you all please fill in the following information for me? Thanks in advance. I surely do appreciate each of you. Joyce Number of pathologists______ Number of cases_____ Number of blocks______ Number of slides_____ Number of special stains______ Number of immunos_______ Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From lesley.bechtold <@t> jax.org Fri Oct 13 11:53:03 2006 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Fri Oct 13 11:54:17 2006 Subject: [Histonet] Opening for a Histologist in Bar Harbor, ME Message-ID: <20061013125303909.00000005988@spikey> There is a fulltime position in the Histology Service of The Jackson Laboratory as a Histotechnologist. Responsibilities include conduct of standard histological protocols including embedding, sectioning and staining as well as routine laboratory maintenance and administrative tasks. Minimum qualifications include Associate's degree in a biological science and HT(ASCP) certification plus 2 years of experience in histology OR a Bachelor's degree in a biological field and 2-3 years of experience in histology. Experience in murine histology and specialized techniques such as serial sectioning, plastic embedding and plastic sectioning is helpful. The incumbent will have the opportunity to further their skills and knowledge. Required computer skills include email, internet, word processing, spreadsheets and familiarity with databases. Effective written and verbal communication skills are essential. Successful applicants will demonstrate good interpersonal skills and must have the ability and willingness to function effectively in a team environment. The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia National Park. Mountains, ocean, forests, lakes, and trails are all within walking distance. If you are looking for a more natural environment, this could be the opportunity you've been searching for. Interested individuals should apply on-line on the internet at www.jax.org, click on Careers in the green bar at the top of the page, then on Search Job Listings in the right-hand column. Refer to job requisition #0545. Please submit cover letter and resume as one document. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From rjbuesa <@t> yahoo.com Fri Oct 13 11:58:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 13 11:59:02 2006 Subject: [Histonet] In memoriam. Message-ID: <20061013165850.65871.qmail@web61216.mail.yahoo.com> On Friday 13 October 1307, exactly 699 years ago today, the troops of the Capetian (French) king Philip IV ["le Bel"] with the benediction of the also French (Avignon) pope Clement V, imprisoned 15,000 members and the 23rd Grand Master of "The Poor Knights of Christ and of the Temple of Salomon" known as the Knights Templar. Both that king and pope owe their principal historical significance to that criminal act but The Knights Templar survived and now are known as "Ordo Supremus Militaris Temple Hierosolymitani" and keep their allegiance to the Roman pope. This brutal act on a Friday the 13 reafirmed some old Nordic / Icelandic traditions about this "evil filled" day that have also survived to our days! Ren? J. --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From Anna.Inman <@t> stmarygj.org Fri Oct 13 12:19:35 2006 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Fri Oct 13 12:20:12 2006 Subject: [Histonet] cap question regarding the microwave Message-ID: <2925AE271EAAD440AF48FCCEB8002D0901DD645C@smgmail01.smgj.sclhs.net> I had asked CAP about this question ..........it makes sense to monitor reproducibility with regards to tissue processing but CAP would not comment on microwaves used for say simply heating agar for cell blocks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 13, 2006 10:21 AM To: srishan@mail.holyname.org; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cap question regarding the microwave Nirmala: As you know, QA records require procedural consistency. Some laboratory designed microwave ovens (MWO) can monitor temperature and, as a matter of fact, the program sets the desired temp. and the time, "leaving" the MWO to set the energy level to reach both requirements. Those MWO usually can print a protocol log in which temp. is recorded. If the MWO is not lab designed, or if it does not have the recording capability, the temp has to be determined with a probe. The whole idea is to make sure that the tissues are subjected only to a pre-determined temp, in the same way that each day the temp. of the retort on any regular tissue processor is recorded. The CAP requirement is that there should be a record, either automatic or logged, with the temp. the tissues have been exposed during the processing protocol. Ren? J. srishan@mail.holyname.org wrote: Hi, I would like to get some feed back on this cap question ANP. 28290 ARE MICROWAVE DEVICES PERIODICALLY MONITORED FOR TEMPERATURE REPRODUCIBILITY? THANKS NIRMALA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From maa8 <@t> cornell.edu Fri Oct 13 13:17:31 2006 From: maa8 <@t> cornell.edu (Mary A. Ascenzi) Date: Fri Oct 13 13:19:21 2006 Subject: [Histonet] Opening for a Histologist in Bar Harbor, ME In-Reply-To: <20061013125303909.00000005988@spikey> References: <20061013125303909.00000005988@spikey> Message-ID: I lived in Bar Harbor and used to ride my bike or use my crosscountry skis to get to my job at Jackson Lab. If you are an outdoor enthusiast, Bar Harbor is a great place to live. I left because I was in my 20s and wanted to see more of the world before I settled down. Mary Ascenzi >There is a fulltime position in the Histology Service of The Jackson >Laboratory as a Histotechnologist. Responsibilities include conduct >of standard histological protocols including embedding, sectioning >and staining as well as routine laboratory maintenance and >administrative tasks. Minimum qualifications include Associate's >degree in a biological science and HT(ASCP) certification plus 2 >years of experience in histology OR a Bachelor's degree in a >biological field and 2-3 years of experience in histology. >Experience in murine histology and specialized techniques such as >serial sectioning, plastic embedding and plastic sectioning is >helpful. The incumbent will have the opportunity to further their >skills and knowledge. Required computer skills include email, >internet, word processing, spreadsheets and familiarity with >databases. Effective written and verbal communication skills are >essential. Successful applicants will demonstrate good >interpersonal skills and must have the ability and willingness to >function effectively in a team environment. > >The Jackson Laboratory is one of the world's foremost centers for >mammalian genetics research. Located in Bar Harbor, Maine, the lab >is adjacent to Acadia National Park. Mountains, ocean, forests, >lakes, and trails are all within walking distance. If you are >looking for a more natural environment, this could be the >opportunity you've been searching for. > >Interested individuals should apply on-line on the internet at >www.jax.org, click on Careers in the green bar at the top of the >page, then on Search Job Listings in the right-hand column. Refer >to job requisition #0545. Please submit cover letter and resume as >one document. > > >Lesley S. Bechtold >Senior Manager, Histopathology & Microscopy Sciences >The Jackson Laboratory >600 Main St. >Bar Harbor, ME 04609 >207-288-6322 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Oct 13 13:22:29 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Oct 13 13:22:37 2006 Subject: [Histonet] PA Histotechnology Society Fall Symposium Message-ID: <6.2.5.6.2.20061013134617.01c65258@vet.upenn.edu> Good Afternoon, We have been updating HistoNet for the last few months we are having our Fall Symposium October 26th, 27th and 28th in Cranberry PA near Pittsburgh. We still space available if you wish to join us for a fun meeting with a variety of seminars and workshops. We have everything from autopsy through bog mummies, IHC for beginners and advanced classes and frozen sectioning for general and Mohs surgery. Want to learn about entomology and forensics or double and triple IHC reactions we have them. Thursday will be devoted to only three seminars for learning more about CAP Inspections and CPT Coding and Ergonomics in Histology. Please see the list below or go to the web site at www.pahisto.org for a full program with abstracts and the registration forms for the meeting. If you have any questions please contact either me (contact info below) or Gloria Limetti (President). Gloria can be reached at glorialimetti@yahoo.com or 412-647-8532 (Work number) for assistance. Join us and the 30 plus vendors for a fun and exciting meeting!! Thursday October 26 ? Special Management Seminars This day is designed for Supervisors and Managers to assist in some of the special areas we are all asked to become experts in today with limited resources and opportunities to attend local educational events. If you are a new manager or just need a refresher with updates join us!! WS# 1 8:00AM to 11:30AM CPT Coding - Which Code Do I Use? Whitaker WS# 2 1:00PM to 4:30PM Preparing for a CAP Inspection Nocito & Hernandez WS# 3 4:30PM to 6:30PM The SWEET Workshop Smart Working Environment Ergonomics Training Minshew Friday Oct 27 - Morning Sessions WS# 4 8:00AM to 9:30 AM Richmond Introduction to Fine Needle Aspiration for the Histotechnologist WS# 5 10:00AM to 11:30 AM Histology Laboratory Workload Measurement: Evaluating Complexity and Productivity Schmitt WS# 6 8:00AM to 11:30 AM Don't Let Immunos Intimdate You!! Whitaker Beginner and Refresher WS# 7 8:00AM to 9:30 AM Safety a Priority in Our Lives Casey WS# 8 10:00AM to 11:30 AM Recycling for Histology Welch WS# 9 8:00AM to 11:30AM Praet Unlocking the Secrets of Mohs? Grossing and Cryosectioning Friday October 27 ? Afternoon Sessions WS#10 1:00PM to 2:30 PM Smart Shopping and Contracts for Histology Macrea WS# 11 1:00PM to 2:30 PM Detection and Amplification of Nucleic Acids in Morphologically Preserved Cells and Tissues Gore WS# 12 1:00PM to 2:30PM Automated Stains Workshop with Multiple Vendors (15 minutes each) WS# 13 3:00PM to 4:30 PM The Bog People of Northern Europe Olsen WS#14 1:00PM to 4:30 PM Nocito & Grossing Surgical Specimens: A Histologist View Hernandez WS# 15 1:00PM to 4:30 PM Peters Frozen Section Techniques New Methods for Cryo-Embedding Saturday October 28 - Morning Sessions WS#16 8:00AM to 11:30 AM Brave New World: An Introduction to Molecular Pathology Haas WS#17 8:00AM to 11:30 AM Meyers Wet Workshop: Multi-antigen Immunohistochemistry (IHC) Staining WS#18 8:00AM to 11:30 AM The Art of Forensic Autopsy Mancuso WS #19 8:00AM to 4:30AM QIHC Readiness and Troubleshooting Macrea WS# 20 8:00AM to 4:30PM HT (ASCP) Examination Readiness Micciche Saturday October 28 Afternoon Sessions WS# 21 1:00PM to 2:30 PM What Plastic Do I Embed This Project In? Marcum WS# 22 1:00PM to 2:30 PM Specimen Labeling and Identification Bar Codes TBA WS#23 1:00PM to 2:30 PM Forensic Insect Entomology Todaro Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From nancy.troiano <@t> yale.edu Fri Oct 13 12:17:38 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Fri Oct 13 13:25:02 2006 Subject: [Histonet] cryosections of rat bone Message-ID: <5.2.1.1.2.20061013131226.00c4d090@email.med.yale.edu> We use the 4X slides from instrumedics and flash twice (flash once, leave the slide in the cryostat chamber while you cut another slide or two and then reflash the original slide - make sure you wait 30-40 seconds between flashes or you'll ruin your unit). Also, remove the tape after the slide warms up slightly or place the slide on the back of your hand to warm it up before pulling off the tape. This procedure should help but so far we've found that we can never get all of the bone to transfer and we've tried a lot of different combinations to get that bone to stick to the slide! From JWEEMS <@t> sjha.org Fri Oct 13 13:42:10 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 13 13:42:35 2006 Subject: [Histonet] Workload comparison Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE5F@sjhaexc02.sjha.org> I was just reminded... I should have said YTD time frame on this little venture! I am assuming that is what you have given me. If its last years that's ok too! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, October 13, 2006 12:40 PM To: Histonet Subject: [Histonet] Workload comparison We have consultant's on site! Would you all please fill in the following information for me? Thanks in advance. I surely do appreciate each of you. Joyce Number of pathologists______ Number of cases_____ Number of blocks______ Number of slides_____ Number of special stains______ Number of immunos_______ Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Maxim_71 <@t> mail.ru Fri Oct 13 13:37:36 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Oct 13 14:06:09 2006 Subject: [Histonet] (no subject) Message-ID: <167753578.20061013223736@mail.ru> We use for bone IPA as dehydratant and "white-spirit" as clearant, then wax. It is probably the same, which mentioned Rene. We do H&E and HC for different bones. We do not IHC. Maxim Peshkov, histotechnologist Department of biopsy and cytological research Pathological and anatomical bureau Taganrog Russia, From tbraud <@t> holyredeemer.com Fri Oct 13 15:57:45 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Oct 13 15:59:16 2006 Subject: [Histonet] cap question regarding the microwave Message-ID: On Microwaves and reproducibility - We use our Kenmore (straight from the shelves of Sears) to heat various stains. This worked for my last inspection. We devised a simple test that we perform monthly. Use 3 identical glass coplin jars, each filled with 50.mls room temp DI water. We check and record the room temp in each. Then one by one, microwave each jar in the center of the microwave on high for 15 seconds, then immediately check and record the heated temp as we remove the jar from the oven. We've never had more than 1 degree variance in the 3 jars or from month to month. Simple and seems to meet their requirement. My Friday flame...am I the only one that thinks that CAP is getting carried away with its AP regs? It seems that they add more and more every revision! Just my 2 cents, Terri Braud -----Original Message----- srishan@mail.holyname.org Sent: Friday, October 13, 2006 11:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cap question regarding the microwave Hi, I would like to get some feed back on this cap question ANP. 28290 ARE MICROWAVE DEVICES PERIODICALLY MONITORED FOR TEMPERATURE REPRODUCIBILITY? THANKS NIRMALA CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From Rcartun <@t> harthosp.org Sun Oct 15 08:46:57 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Oct 15 08:47:43 2006 Subject: [Histonet] Vison BioSystems - Peloris Message-ID: <453203910200007700002610@hcnwgwds01.hh.chs> Anyone out there using Vison BioSystems' "Peloris" for tissue processing? If so, what has been your experience? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From lhotaks <@t> mcmaster.ca Sun Oct 15 12:54:02 2006 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Sun Oct 15 12:54:30 2006 Subject: [Histonet] decorin antibody Message-ID: Hello Netters, Is anybody staining mouse tissue, preferable paraffin sections, for decorin? I would appreciate your help with locating a suitable antibody. Thank you, Sarka Lhotak McMaster University, Hamilton, Ontario From Pathrm35 <@t> adelphia.net Sun Oct 15 15:13:39 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Sun Oct 15 15:13:44 2006 Subject: [Histonet] Vison BioSystems - Peloris In-Reply-To: <453203910200007700002610@hcnwgwds01.hh.chs> Message-ID: <000601c6f096$67539790$2ea2ac45@D6WRV2B1> Richard, We have been using the Peloris for about two months now. We are a dermpath lab and our biopsies are mostly shaves, punches, excisions, cysts and lipomas. We run a 1.5 hour or a two hour run for our small cases (2 runs a day) and a 5 or 6 hour (overnight) run for our larger cases. Everything processes well and the machine is easy to use. Our immunos stain well with this processor and we use the Vision Biosystem Bond IHC system. I had the opportunity to go to Mass. for training with Rick Couture and it was a very pleasant, professional experience. In South Florida we have a very good Field Service Specialist named Steve Westra. Overall I have had a very good experience with the Peloris and this company. Just my two cents worth. Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Sunday, October 15, 2006 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vison BioSystems - Peloris Anyone out there using Vison BioSystems' "Peloris" for tissue processing? If so, what has been your experience? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Oct 16 10:21:59 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 16 10:22:36 2006 Subject: [Histonet] cryosectioning rat bone In-Reply-To: Message-ID: <004801c6f136$d5e5e820$6601a8c0@Patsy> John, I have used the Instrumedics tape transfer system for frozen rat bones for years and I am yet to get all of cortical bone to stay attached to the slide and not come off with the tape. Here is my best effort: I infiltrate the samples in 30% sucrose overnight before snap freezing, I use a tungsten carbide D profile knife of course, I cut the section as thin as possible (I shoot for 4 microns), then I really spend a lot of time and pressure rolling the section onto the coated slide, flash it with UV about 3 times, then before removing the tape I lay the slide flat on a block of dry ice and let it get really cold before slowly with even pulling remove the tape starting at a corner and pulling diagonally from corner to corner. John Trapley wrote a good how too article for JOH a several years ago which I contributed to, on this. Gayle Callis is another with experience and Diane Sterchi developed a method using another 3M tape I believe. This method is a good sent for cutting mineralized bone in the cryostat but it is not perfect or at least I have not ever gotten a completely perfect section with all the bone intact left on the coated slide. Diane send me the ordering info for that tape please, if you are out there. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Baker Sent: Thursday, October 12, 2006 11:33 AM To: Histonet Cc: ewaldorf@engin.umich.edu Subject: [Histonet] cryosectioning rat bone Hi All, Does anyone out there in histoland get good cryo-sections of rat femur or tibia? I get great 5-7 micron sections of mouse bone using the CryoJane Tape System. Whether longitudinal or cross the cortical bone will not laminate and comes off with the window. I certainly don't get sections at all using the anti-roll plate. I have a Hacker-Bright OTF cryostat and use t-c blades. Any suggestions are greatly appreciated. Thanks in advance. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology-consultants <@t> hotmail.com Mon Oct 16 14:53:33 2006 From: histology-consultants <@t> hotmail.com (Gregor Arlt) Date: Mon Oct 16 14:53:47 2006 Subject: [Histonet] Milestone Pathos vs Sakura Xpress Message-ID: Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ From jestrong <@t> comcast.net Mon Oct 16 15:43:55 2006 From: jestrong <@t> comcast.net (Jes Strong) Date: Mon Oct 16 15:44:05 2006 Subject: [Histonet] Milestone Pathos vs Sakura Xpress In-Reply-To: Message-ID: <00a501c6f163$cc5b0010$0200a8c0@Jes> Dear Mr. Arlt, If you would like to contact Milestone directly, we would be happy to explain the principles of Milestone's microwave processors including magnetron output and how it is dynamically regulated by software to adjust to each specific load. These are not questions that users would, or should be expected to be able to answer for you satisfactorily. Jes Strong Western Region Sales Manager Milestone Medical (203) 925-4240 (Office) (847) 323-8373 (Cell) (847) 655-6009 (Fax) jes@milestonemed.com www.milestonemed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregor Arlt Sent: Monday, October 16, 2006 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Milestone Pathos vs Sakura Xpress Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Oct 16 16:19:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 16 16:19:24 2006 Subject: [Histonet] Milestone Pathos vs Sakura Xpress In-Reply-To: Message-ID: <20061016211918.92177.qmail@web61214.mail.yahoo.com> Frank: The temperature produced by the magnetron is "controlled" by the temperature probe adjusted to the processing protocol; you can have 1200W and a working temperature of 50?C so wattage in itself is not a deletereous agent just provides the capability of getting to high temperatures quickly, if needed.. Not all technologies are alike and the Xpress uses 60W continuously in the first 2 chambers. PATHOS is pure microwave technology and Xpress is a blend of MW and conventional technology. In the Xpress the first 2 chambers are identical and using MW technology and bubble agitation. The other 2 are just 2 conventional retorts with convection heat and vacuum capabilities, operated at 65?C. The molecular integrity does not relate to the process but to the tissue fixation. Formalin greatly prevents RNA studies but any alcoholic fixative like Kryofix, BoonFix or the propietary by Sakura (UMFix) will preserve the macromolecules either if the tissue is going to be processed with MW or conventional technology. Any good alcoholic fixative containin PEG also will preserve the macromolecules. Both PATHOS and Xpress are "walk away" instruments but Xpress allows for the continuous addition of up to 30 cassettes every 15 minutes, for an overall work flow of 120 cassettes after 105 minutes, and 30 more every 15 minutes afterwards. The limit is the thickness of the sections (have to be 1.5mm thick) and some tissues have to be previously fixed from 4 to 4.5 hours before processing. PATHOS can process tissues of up to 5mm at a rate of 210 cassettes/4 hours (fixation included) for thick tissue slices or 210 cassettes/1 hour for small biopsies. Hope this information will help you! Ren? J. Gregor Arlt wrote: Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From jqb7 <@t> cdc.gov Mon Oct 16 17:46:57 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Oct 16 19:06:41 2006 Subject: [Histonet] Milestone Pathos vs Sakura Xpress References: Message-ID: Frank, I have no experience with the Pathos but our lab does have the Sakura Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 explains how the microwave itself operates (60 watts). One thing I want to add to the previous response is that the 1.5 mm thickness for the Xpress is not mandatory. There is allowance for thicker specimens but the processing time is then lengthened. But I for one like having an excuse to make pathologists gross properly to begin with. :) Jeanine Bartlett CDC, Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gregor Arlt Sent: Mon 10/16/2006 3:53 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Milestone Pathos vs Sakura Xpress Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srwilkes <@t> gmail.com Mon Oct 16 21:15:25 2006 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Mon Oct 16 21:15:33 2006 Subject: [Histonet] seeking hiotology position in DC area Message-ID: <8e5827cf0610161915w1f450489mb6aec23f432807ae@mail.gmail.com> Hello I am seeking a histology position in the greater DC area. I have a MA in biology, 2+ years of histology and immunohistochemistry experience, as well as a bit histology teaching. Thank you Steven From Tony_Reilly <@t> health.qld.gov.au Mon Oct 16 21:37:52 2006 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Oct 16 21:45:18 2006 Subject: [Histonet] Vison BioSystems - Peloris Message-ID: Hi Richard My peloris was part of the very first relase of the instrument. As a result there were some initial minor problems which have since been rectified. As I said these problems were minor and never in any way affected the processing of our tissue. It now runs very well and has had a positive impact on our laboratory. The instrument has a very powerful mixing ability which not only improves penetration but guarantees even heating on the steps where heat is utilised giving shorter processing times even for fatty tissue. This is aided by using one of their range of specimen baskets which separates each cassette individually allowing better flow of solution to each specimen. Our laboratory services both heart and lung transplant units requiring us to run numeroous short cycles throughout the day. The improved processing combined with a rapid clean cycle means that the one dual retort peloris can do the work of 3-4 of our prevoius instruments. Examples of our improved times include: Fatty tissue 18h to 14h Routine 12h to 9h Small Biopsy 2h to 1 h This has also had an impact on IHC as the small biopsies that are required urgently can be given an extra 1h in formalin and still be completed in the same time as the previous protocol. With the faster processing some tissues such as lletz biopsies need to be processed on shorter cycles to avoid hardening of the tissue. According to the manufacturer these times can be reduced further by substituting xylene with isopropanol but I have not tried that so cannot comment. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "Richard Cartun" 10/15/06 11:46 pm >>> Anyone out there using Vison BioSystems' "Peloris" for tissue processing? If so, what has been your experience? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From yjones2 <@t> csmlab.com Tue Oct 17 01:43:21 2006 From: yjones2 <@t> csmlab.com (Yvonne Jones) Date: Tue Oct 17 01:44:04 2006 Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog Message-ID: <453443490200004F00000225@GWGATE1.ahm.com> Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our pathologists have recently requested that we begin testing an antibody, p52. I have had a bear of a time finding any information about this antibody!!! All I could find was one article on-line, and I am still having a problem finding info and the antibody itself. ----------------------------------------- From jqb7 <@t> cdc.gov Tue Oct 17 04:49:06 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Oct 17 05:00:51 2006 Subject: [Histonet] Milestone Pathos vs Sakura Xpress Message-ID: Apparently the PDF was too large for Histonet to accept. You can go to the Sakura website and click on Xpress and open the brochure if you need these details. Jeanine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Monday, October 16, 2006 6:47 PM To: Gregor Arlt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress Frank, I have no experience with the Pathos but our lab does have the Sakura Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 explains how the microwave itself operates (60 watts). One thing I want to add to the previous response is that the 1.5 mm thickness for the Xpress is not mandatory. There is allowance for thicker specimens but the processing time is then lengthened. But I for one like having an excuse to make pathologists gross properly to begin with. :) Jeanine Bartlett CDC, Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gregor Arlt Sent: Mon 10/16/2006 3:53 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Milestone Pathos vs Sakura Xpress Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rdavis4 <@t> rdg.boehringer-ingelheim.com Tue Oct 17 06:46:17 2006 From: rdavis4 <@t> rdg.boehringer-ingelheim.com (rdavis4@rdg.boehringer-ingelheim.com) Date: Tue Oct 17 06:46:41 2006 Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog In-Reply-To: <453443490200004F00000225@GWGATE1.ahm.com> Message-ID: <83BA2D3D42947D48BDAA449453644ABE0865B1@RDGEXM01.am.boehringer.com> Yvonne, Google p52 and lots of hits come up. Also, check out www.abcam.com. They have p52 as a rabbit polyclonal. Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) Toxicology, Histopathology Lab Boehringer-Ingelheim Pharmaceuticals, Inc. rdavis4@rdg.boehringer-ingelheim.com 203-798-5448 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones Sent: Tuesday, October 17, 2006 2:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our pathologists have recently requested that we begin testing an antibody, p52. I have had a bear of a time finding any information about this antibody!!! All I could find was one article on-line, and I am still having a problem finding info and the antibody itself. ----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From soofias2 <@t> yahoo.com Tue Oct 17 09:39:31 2006 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Tue Oct 17 09:39:44 2006 Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog In-Reply-To: <83BA2D3D42947D48BDAA449453644ABE0865B1@RDGEXM01.am.boehringer.com> Message-ID: <20061017143931.16702.qmail@web39513.mail.mud.yahoo.com> Go to Google and go check for Biocompare.com. Biocompare is a very good source to search for any bio products. I have searched and found all of the antibodies, that Dako has discontinued, through this web site. Good luck! Soofia rdavis4@rdg.boehringer-ingelheim.com wrote: Yvonne, Google p52 and lots of hits come up. Also, check out www.abcam.com. They have p52 as a rabbit polyclonal. Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) Toxicology, Histopathology Lab Boehringer-Ingelheim Pharmaceuticals, Inc. rdavis4@rdg.boehringer-ingelheim.com 203-798-5448 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones Sent: Tuesday, October 17, 2006 2:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our pathologists have recently requested that we begin testing an antibody, p52. I have had a bear of a time finding any information about this antibody!!! All I could find was one article on-line, and I am still having a problem finding info and the antibody itself. ----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From prippstein <@t> ottawaheart.ca Tue Oct 17 10:36:56 2006 From: prippstein <@t> ottawaheart.ca (Peter Rippstein) Date: Tue Oct 17 10:37:28 2006 Subject: [Histonet] heat antigen retrieval methods Message-ID: Hello Histonetters, Our lab is in need of some information in regards heat antigen retrieval methods. Has anyone done a comparison in terms of results & cost effectiveness obtained from microwave irradiation vs pressure cooking and steam heating methods. Any recommendations would be appreciated. Many thanks. Peter Peter Rippstein ART, MLT Core Pathology Laboratory Rm H2102 University of Ottawa Heart Institute 40 Ruskin Street Ottawa, Ontario Canada, K1Y 4W7 Tel: (613) 761-5282 Fax: (613) 761-5281 Email: prippstein@ottawaheart.ca -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD From Martina.Urbanek <@t> uibk.ac.at Tue Oct 17 10:51:41 2006 From: Martina.Urbanek <@t> uibk.ac.at (Martina Urbanek) Date: Tue Oct 17 10:51:55 2006 Subject: [Histonet] problems with mouse brain fixation Message-ID: <1161100301.4534fc0d23f8a@web-mail1.uibk.ac.at> Hello everybody on histonet, we have some problems with handling mouse (21 and 90 days old) and rat brains. We do mouse brain perfusion using 4% paraformaldehyde in PBS, pH 7.4. We use gravity for perfusion for about 30 minutes (volume about 80 ml) and after perfusion we leave the brains over night in the same fixative. Then we process in a Shandon tissue processor (70% Alcohol, 80% Alcohol, 95% Alcohol, 3 changes 100% alcohol, 3 changes xylene, 2 changes paraffin 56?C; time depends on size of tissue). Now we have the problem that some brains are too hard and some also seem to shrink more than others, when we cut them and put them on waterbath they seem to expand and distort and often brittle. So that it looks like only fibrous tissue is left, the structure is gone. I already had a look on histonet archive but did not find anything that helps, therefore I hope that someone has an idea what can be wrong. I have to say that we don?t have any problems when we immersion fix the brains with 4% formaldehyde. The problems we have with rat brains are a bit different, because we get the brains from another group who perfuse the lung with 4% paraformaldehyde in Hepes-buffer, pH 7.35. We then postfix over night in the same fixative they use (when the brain is also perfused) or for 3 days (when brain is not perfused). After dehydration in tissue processor we have the same cutting problems like we observe with mouse brain tissue, even worse. Any suggestions are appreciated! Thank you very much for your help!!! Martina Urbanek Ms. Martina Urbanek Forschungslabor der Klin.Abt. f?r Neonatologie neonatal neuroscience research laboratory Med. University Innsbruck Innrain 66, 4th floor A-6020 Innsbruck Tel. +43 (0)512 504 27755/27765 Fax: +43 (0)512 504 27766 Email: Martina.Urbanek@uibk.ac.at From GoodwinD <@t> pahosp.com Tue Oct 17 11:52:24 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Tue Oct 17 11:52:37 2006 Subject: [Histonet] Phospho-S6 Ribosomal Protein Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8235@uphsmbx2.UPHS.PENNHEALTH.PRV> For those of us 'Netters using this Ab on human tissue, which one and at what dilution? Thanks! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From GoodwinD <@t> pahosp.com Tue Oct 17 13:14:30 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Tue Oct 17 13:14:57 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 28, heat antigen retrieval methods Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8237@uphsmbx2.UPHS.PENNHEALTH.PRV> Peter: Check out the June 2006 issue of the Medical Laboratory Observer(MLO)-www.mlo-online.com. The cover story was on AR. Diana Goodwin Pennsylvania Hospital Philadelphia, PA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, October 17, 2006 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 35, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Milestone Pathos vs Sakura Xpress (Gregor Arlt) 2. RE: Milestone Pathos vs Sakura Xpress (Jes Strong) 3. Re: Milestone Pathos vs Sakura Xpress (Rene J Buesa) 4. RE: Milestone Pathos vs Sakura Xpress (Bartlett, Jeanine (CDC/CCID/NCZVED)) 5. seeking hiotology position in DC area (Steven Wilkes) 6. Re: Vison BioSystems - Peloris (Anthony Reilly) 7. Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog (Yvonne Jones) 8. RE: Milestone Pathos vs Sakura Xpress (Bartlett, Jeanine (CDC/CCID/NCZVED)) 9. RE: Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog (rdavis4@rdg.boehringer-ingelheim.com) 10. RE: Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog (soofia siddiqui) 11. heat antigen retrieval methods (Peter Rippstein) 12. problems with mouse brain fixation (Martina Urbanek) 13. Phospho-S6 Ribosomal Protein (Goodwin, Diana) ---------------------------------------------------------------------- Message: 1 Date: Mon, 16 Oct 2006 14:53:33 -0500 From: "Gregor Arlt" Subject: [Histonet] Milestone Pathos vs Sakura Xpress To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ ------------------------------ Message: 2 Date: Mon, 16 Oct 2006 15:43:55 -0500 From: "Jes Strong" Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress To: Message-ID: <00a501c6f163$cc5b0010$0200a8c0@Jes> Content-Type: text/plain; charset="us-ascii" Dear Mr. Arlt, If you would like to contact Milestone directly, we would be happy to explain the principles of Milestone's microwave processors including magnetron output and how it is dynamically regulated by software to adjust to each specific load. These are not questions that users would, or should be expected to be able to answer for you satisfactorily. Jes Strong Western Region Sales Manager Milestone Medical (203) 925-4240 (Office) (847) 323-8373 (Cell) (847) 655-6009 (Fax) jes@milestonemed.com www.milestonemed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregor Arlt Sent: Monday, October 16, 2006 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Milestone Pathos vs Sakura Xpress Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 16 Oct 2006 14:19:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Milestone Pathos vs Sakura Xpress To: Gregor Arlt , histonet@lists.utsouthwestern.edu Message-ID: <20061016211918.92177.qmail@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Frank: The temperature produced by the magnetron is "controlled" by the temperature probe adjusted to the processing protocol; you can have 1200W and a working temperature of 50?C so wattage in itself is not a deletereous agent just provides the capability of getting to high temperatures quickly, if needed.. Not all technologies are alike and the Xpress uses 60W continuously in the first 2 chambers. PATHOS is pure microwave technology and Xpress is a blend of MW and conventional technology. In the Xpress the first 2 chambers are identical and using MW technology and bubble agitation. The other 2 are just 2 conventional retorts with convection heat and vacuum capabilities, operated at 65?C. The molecular integrity does not relate to the process but to the tissue fixation. Formalin greatly prevents RNA studies but any alcoholic fixative like Kryofix, BoonFix or the propietary by Sakura (UMFix) will preserve the macromolecules either if the tissue is going to be processed with MW or conventional technology. Any good alcoholic fixative containin PEG also will preserve the macromolecules. Both PATHOS and Xpress are "walk away" instruments but Xpress allows for the continuous addition of up to 30 cassettes every 15 minutes, for an overall work flow of 120 cassettes after 105 minutes, and 30 more every 15 minutes afterwards. The limit is the thickness of the sections (have to be 1.5mm thick) and some tissues have to be previously fixed from 4 to 4.5 hours before processing. PATHOS can process tissues of up to 5mm at a rate of 210 cassettes/4 hours (fixation included) for thick tissue slices or 210 cassettes/1 hour for small biopsies. Hope this information will help you! Ren? J. Gregor Arlt wrote: Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. ------------------------------ Message: 4 Date: Mon, 16 Oct 2006 18:46:57 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress To: "Gregor Arlt" , Message-ID: Content-Type: text/plain; charset="UTF-8" Frank, I have no experience with the Pathos but our lab does have the Sakura Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 explains how the microwave itself operates (60 watts). One thing I want to add to the previous response is that the 1.5 mm thickness for the Xpress is not mandatory. There is allowance for thicker specimens but the processing time is then lengthened. But I for one like having an excuse to make pathologists gross properly to begin with. :) Jeanine Bartlett CDC, Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gregor Arlt Sent: Mon 10/16/2006 3:53 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Milestone Pathos vs Sakura Xpress Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 16 Oct 2006 22:15:25 -0400 From: "Steven Wilkes" Subject: [Histonet] seeking hiotology position in DC area To: histonet@lists.utsouthwestern.edu Message-ID: <8e5827cf0610161915w1f450489mb6aec23f432807ae@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello I am seeking a histology position in the greater DC area. I have a MA in biology, 2+ years of histology and immunohistochemistry experience, as well as a bit histology teaching. Thank you Steven ------------------------------ Message: 6 Date: Tue, 17 Oct 2006 12:37:52 +1000 From: "Anthony Reilly" Subject: Re: [Histonet] Vison BioSystems - Peloris To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Richard My peloris was part of the very first relase of the instrument. As a result there were some initial minor problems which have since been rectified. As I said these problems were minor and never in any way affected the processing of our tissue. It now runs very well and has had a positive impact on our laboratory. The instrument has a very powerful mixing ability which not only improves penetration but guarantees even heating on the steps where heat is utilised giving shorter processing times even for fatty tissue. This is aided by using one of their range of specimen baskets which separates each cassette individually allowing better flow of solution to each specimen. Our laboratory services both heart and lung transplant units requiring us to run numeroous short cycles throughout the day. The improved processing combined with a rapid clean cycle means that the one dual retort peloris can do the work of 3-4 of our prevoius instruments. Examples of our improved times include: Fatty tissue 18h to 14h Routine 12h to 9h Small Biopsy 2h to 1 h This has also had an impact on IHC as the small biopsies that are required urgently can be given an extra 1h in formalin and still be completed in the same time as the previous protocol. With the faster processing some tissues such as lletz biopsies need to be processed on shorter cycles to avoid hardening of the tissue. According to the manufacturer these times can be reduced further by substituting xylene with isopropanol but I have not tried that so cannot comment. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "Richard Cartun" 10/15/06 11:46 pm >>> Anyone out there using Vison BioSystems' "Peloris" for tissue processing? If so, what has been your experience? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** ------------------------------ Message: 7 Date: Tue, 17 Oct 2006 02:43:21 -0400 From: "Yvonne Jones" Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog To: Message-ID: <453443490200004F00000225@GWGATE1.ahm.com> Content-Type: text/plain; charset="US-ASCII" Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our pathologists have recently requested that we begin testing an antibody, p52. I have had a bear of a time finding any information about this antibody!!! All I could find was one article on-line, and I am still having a problem finding info and the antibody itself. ----------------------------------------- ------------------------------ Message: 8 Date: Tue, 17 Oct 2006 05:49:06 -0400 From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , "Gregor Arlt" , Message-ID: Content-Type: text/plain; charset="us-ascii" Apparently the PDF was too large for Histonet to accept. You can go to the Sakura website and click on Xpress and open the brochure if you need these details. Jeanine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Monday, October 16, 2006 6:47 PM To: Gregor Arlt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress Frank, I have no experience with the Pathos but our lab does have the Sakura Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 explains how the microwave itself operates (60 watts). One thing I want to add to the previous response is that the 1.5 mm thickness for the Xpress is not mandatory. There is allowance for thicker specimens but the processing time is then lengthened. But I for one like having an excuse to make pathologists gross properly to begin with. :) Jeanine Bartlett CDC, Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gregor Arlt Sent: Mon 10/16/2006 3:53 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Milestone Pathos vs Sakura Xpress Dear Histonetters, I have heard the Pathos would use 600 Watts for the microwave processing. I can't imagine that this is right. In my opinion this would destroy the RNS structure of any tissue. On the other hand I heard tissues processed in the Xpress would be easy to use for molecularbiological investigations. Has anybody experience with those instruments, or know anybody the power of the microwave of the instruments. Thanks for the help Frank _________________________________________________________________ Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 17 Oct 2006 07:46:17 -0400 From: rdavis4@rdg.boehringer-ingelheim.com Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog To: yjones2@csmlab.com, histonet@lists.utsouthwestern.edu Message-ID: <83BA2D3D42947D48BDAA449453644ABE0865B1@RDGEXM01.am.boehringer.com> Content-Type: text/plain; charset=us-ascii Yvonne, Google p52 and lots of hits come up. Also, check out www.abcam.com. They have p52 as a rabbit polyclonal. Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) Toxicology, Histopathology Lab Boehringer-Ingelheim Pharmaceuticals, Inc. rdavis4@rdg.boehringer-ingelheim.com 203-798-5448 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones Sent: Tuesday, October 17, 2006 2:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our pathologists have recently requested that we begin testing an antibody, p52. I have had a bear of a time finding any information about this antibody!!! All I could find was one article on-line, and I am still having a problem finding info and the antibody itself. ----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 17 Oct 2006 07:39:31 -0700 (PDT) From: soofia siddiqui Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog To: rdavis4@rdg.boehringer-ingelheim.com, yjones2@csmlab.com, histonet@lists.utsouthwestern.edu Message-ID: <20061017143931.16702.qmail@web39513.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Go to Google and go check for Biocompare.com. Biocompare is a very good source to search for any bio products. I have searched and found all of the antibodies, that Dako has discontinued, through this web site. Good luck! Soofia rdavis4@rdg.boehringer-ingelheim.com wrote: Yvonne, Google p52 and lots of hits come up. Also, check out www.abcam.com. They have p52 as a rabbit polyclonal. Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) Toxicology, Histopathology Lab Boehringer-Ingelheim Pharmaceuticals, Inc. rdavis4@rdg.boehringer-ingelheim.com 203-798-5448 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones Sent: Tuesday, October 17, 2006 2:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our patholog Hello, fellow histotechies!!! This is my first time posing a question - so be gentle. Our pathologists have recently requested that we begin testing an antibody, p52. I have had a bear of a time finding any information about this antibody!!! All I could find was one article on-line, and I am still having a problem finding info and the antibody itself. ----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com ------------------------------ Message: 11 Date: Tue, 17 Oct 2006 11:36:56 -0400 From: "Peter Rippstein" Subject: [Histonet] heat antigen retrieval methods To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters, Our lab is in need of some information in regards heat antigen retrieval methods. Has anyone done a comparison in terms of results & cost effectiveness obtained from microwave irradiation vs pressure cooking and steam heating methods. Any recommendations would be appreciated. Many thanks. Peter Peter Rippstein ART, MLT Core Pathology Laboratory Rm H2102 University of Ottawa Heart Institute 40 Ruskin Street Ottawa, Ontario Canada, K1Y 4W7 Tel: (613) 761-5282 Fax: (613) 761-5281 Email: prippstein@ottawaheart.ca -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Peter Rippstein EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca N:Rippstein;Peter END:VCARD ------------------------------ Message: 12 Date: Tue, 17 Oct 2006 17:51:41 +0200 From: Martina Urbanek Subject: [Histonet] problems with mouse brain fixation To: histonet@lists.utsouthwestern.edu Message-ID: <1161100301.4534fc0d23f8a@web-mail1.uibk.ac.at> Content-Type: text/plain; charset=ISO-8859-1 Hello everybody on histonet, we have some problems with handling mouse (21 and 90 days old) and rat brains. We do mouse brain perfusion using 4% paraformaldehyde in PBS, pH 7.4. We use gravity for perfusion for about 30 minutes (volume about 80 ml) and after perfusion we leave the brains over night in the same fixative. Then we process in a Shandon tissue processor (70% Alcohol, 80% Alcohol, 95% Alcohol, 3 changes 100% alcohol, 3 changes xylene, 2 changes paraffin 56?C; time depends on size of tissue). Now we have the problem that some brains are too hard and some also seem to shrink more than others, when we cut them and put them on waterbath they seem to expand and distort and often brittle. So that it looks like only fibrous tissue is left, the structure is gone. I already had a look on histonet archive but did not find anything that helps, therefore I hope that someone has an idea what can be wrong. I have to say that we don?t have any problems when we immersion fix the brains with 4% formaldehyde. The problems we have with rat brains are a bit different, because we get the brains from another group who perfuse the lung with 4% paraformaldehyde in Hepes-buffer, pH 7.35. We then postfix over night in the same fixative they use (when the brain is also perfused) or for 3 days (when brain is not perfused). After dehydration in tissue processor we have the same cutting problems like we observe with mouse brain tissue, even worse. Any suggestions are appreciated! Thank you very much for your help!!! Martina Urbanek Ms. Martina Urbanek Forschungslabor der Klin.Abt. f?r Neonatologie neonatal neuroscience research laboratory Med. University Innsbruck Innrain 66, 4th floor A-6020 Innsbruck Tel. +43 (0)512 504 27755/27765 Fax: +43 (0)512 504 27766 Email: Martina.Urbanek@uibk.ac.at ------------------------------ Message: 13 Date: Tue, 17 Oct 2006 12:52:24 -0400 From: "Goodwin, Diana" Subject: [Histonet] Phospho-S6 Ribosomal Protein To: , Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8235@uphsmbx2.UPHS.PENNHEALTH.PRV> Content-Type: text/plain; charset="us-ascii" For those of us 'Netters using this Ab on human tissue, which one and at what dilution? Thanks! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 28 **************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From lwhite <@t> prhc.on.ca Tue Oct 17 14:03:20 2006 From: lwhite <@t> prhc.on.ca (White, Lori) Date: Tue Oct 17 14:03:26 2006 Subject: [Histonet] trichrome staining Message-ID: <466A56825600F346A3BA6F436051A8973A84CC@prhc-mx1.prhc.on.ca> Does anybody have a method for trichrome staining that does not use picric acid or mercury for fixation? References would be wonderful if you have them. Thanks, Lori White Peterborough, Ontario Important Notice - This electronic transmission is strictly confidential to Peterborough Regional Health Centre and intended to be delivered only to the addressee. Information contained within the transmission, is protected by legal, professional and/or other privilege. If you are not the intended addressee, or not authorized by the intended addressee to receive this transmission, you must not retain, disclose in any manner, copy, or take any action in reliance on this transmission. If you have received this transmission in error, as a courtesy, please notify the sender as soon as possible and then delete or destroy this message. From Malcolm.McCallum <@t> tamut.edu Tue Oct 17 15:08:56 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Tue Oct 17 15:11:55 2006 Subject: [Histonet] trichrome staining References: <466A56825600F346A3BA6F436051A8973A84CC@prhc-mx1.prhc.on.ca> Message-ID: According to Humason's Animal Tissue Techniques (5th ed.), Pg 126. You can use Masson Trichrome Staining method with any general fixative. Pollak Rapid Method (pg 128) can also use any general fixative, although mercuric chloride will improve formalin fixed tissues. Finally, you can use Gomori's one-step Trichrome as it is intended for use with neutral buffered formalin (pg 129). Hope that helps! VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of White, Lori Sent: Tue 10/17/2006 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] trichrome staining Does anybody have a method for trichrome staining that does not use picric acid or mercury for fixation? References would be wonderful if you have them. Thanks, Lori White Peterborough, Ontario Important Notice - This electronic transmission is strictly confidential to Peterborough Regional Health Centre and intended to be delivered only to the addressee. Information contained within the transmission, is protected by legal, professional and/or other privilege. If you are not the intended addressee, or not authorized by the intended addressee to receive this transmission, you must not retain, disclose in any manner, copy, or take any action in reliance on this transmission. If you have received this transmission in error, as a courtesy, please notify the sender as soon as possible and then delete or destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nienhuis <@t> ucla.edu Tue Oct 17 16:31:41 2006 From: nienhuis <@t> ucla.edu (nienhuis@ucla.edu) Date: Tue Oct 17 16:31:51 2006 Subject: [Histonet] Controls for nickel enhanced DAB In-Reply-To: <466A56825600F346A3BA6F436051A8973A84CC@prhc-mx1.prhc.on.ca> References: <466A56825600F346A3BA6F436051A8973A84CC@prhc-mx1.prhc.on.ca> Message-ID: <20061017143141.mhc97owess4s8kwg@mail.ucla.edu> Is leaving out the primary antibody an adequate control for nickel enhanced DAB IHC? I'm staining for receptors in mouse CNS, and don't see any staining in control sections and some diffuse staining of varying intensity in the experimental tissue. Is All the darker stuff specific staining? Of course I see some stained cells and processes too. Looking to do some quantative IHC on the tissue and this is a new area for me. Can anyone point out some possible pitfalls that I might run into in this endeavor? Bob Nienhuis Neurobiology Research UCLA / VA Medical Center Los Angeles From dassog <@t> evergreen.edu Tue Oct 17 16:55:19 2006 From: dassog <@t> evergreen.edu (Dasso, Greg (staff)) Date: Tue Oct 17 16:56:28 2006 Subject: [Histonet] Capillary analysis by confocal fluorescence microscopy References: <466A56825600F346A3BA6F436051A8973A84CC@prhc-mx1.prhc.on.ca> <20061017143141.mhc97owess4s8kwg@mail.ucla.edu> Message-ID: <3872E8431D06E545AC955BED177CB6F601604ABB@oak.evergreen.edu> Histonetters, I'm throwing this out to the community in hopes that I'll find some creative techniques for capillary analysis. The current project I'm working on involves staining capillaries in unfixed fetal sheep hearts for confocal imaging and vascular network analysis. We've used CD-31 and CD-34 with limited success on mice. Additionally, the polymer casting material we've used for the last few years doesn't offer adequate resolution with respect to autofluorescence at the capillary level, so my PI has asked me to explore other labelling techniques. Initially, I thought that perfusion with an antibody or PFBFDA to stain the complete arterial tree followed by a smooth muscle label would work to differentiate caps from higher-order network structures. Any ideas (obvious or otherwise)? Thanks in advance, Greg Dasso Barlow Scientific, Inc dassog@evergreen.edu From jqb7 <@t> cdc.gov Tue Oct 17 17:52:32 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Oct 17 17:53:13 2006 Subject: [Histonet] trichrome staining References: <466A56825600F346A3BA6F436051A8973A84CC@prhc-mx1.prhc.on.ca> Message-ID: But with the One-Step you must post-mordant with Bouin's. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Malcolm McCallum Sent: Tue 10/17/2006 4:08 PM To: White, Lori; histonet@lists.utsouthwestern.edu Cc: Subject: RE: [Histonet] trichrome staining According to Humason's Animal Tissue Techniques (5th ed.), Pg 126. You can use Masson Trichrome Staining method with any general fixative. Pollak Rapid Method (pg 128) can also use any general fixative, although mercuric chloride will improve formalin fixed tissues. Finally, you can use Gomori's one-step Trichrome as it is intended for use with neutral buffered formalin (pg 129). Hope that helps! VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of White, Lori Sent: Tue 10/17/2006 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] trichrome staining Does anybody have a method for trichrome staining that does not use picric acid or mercury for fixation? References would be wonderful if you have them. Thanks, Lori White Peterborough, Ontario Important Notice - This electronic transmission is strictly confidential to Peterborough Regional Health Centre and intended to be delivered only to the addressee. Information contained within the transmission, is protected by legal, professional and/or other privilege. If you are not the intended addressee, or not authorized by the intended addressee to receive this transmission, you must not retain, disclose in any manner, copy, or take any action in reliance on this transmission. If you have received this transmission in error, as a courtesy, please notify the sender as soon as possible and then delete or destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abishop <@t> medlabcentral.co.nz Tue Oct 17 22:57:57 2006 From: abishop <@t> medlabcentral.co.nz (Alan Bishop) Date: Tue Oct 17 23:02:14 2006 Subject: [Histonet] Dako Eridan Message-ID: Are there any users of the new eridan immuno stainer out there in histo land? We are looking at either the BondMax or the Eridan and personally much prefer the idea of the BondMax as have used one compared to the Eridan where they are saying there will be ONE in Australasia by the end of the year - maybe, and if we want one before 2008 we have to say now and order one now even without seeing the machine as they are only selling two to New Zealand next year! Also, are the general experiences with DAKO getting any better at the moment? So if anyone does have some feedback or any other comments to add I would like to hear them. Cheers and best wishes Alan Bishop Charge scientist Histology Medlab Central Palmerston North Tel: 06 952 3135 Fax: 06 952 3199 From matt.prideaux <@t> bbsrc.ac.uk Wed Oct 18 04:12:17 2006 From: matt.prideaux <@t> bbsrc.ac.uk (matt prideaux (RI)) Date: Wed Oct 18 04:12:36 2006 Subject: [Histonet] Benzoyl peroxide Message-ID: <84DA9D8AC9B05F4B889E7C70238CB45104C52A82@rie2ksrv1.ri.bbsrc.ac.uk> Hi, I'm trying to embed some mouse tibiae in methyl methacrylate, but I'm having no luck in getting the resin to polymerise. After doing some reading I've discovered that it's probably because I didn't dry the benzoyl peroxide first (the stuff we use contains 30% water). Does anyone know of a safe way to dry the benzoyl peroxide? (I've heard lots of horror stories about ovens and explosions!) we don't have a dessicator here but there is a vacuum oven and a freeze drier, would these be ok to use? Thanks Matt The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute. From nancy.troiano <@t> yale.edu Wed Oct 18 06:39:38 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Wed Oct 18 06:39:46 2006 Subject: [Histonet] Fluorochrome labeling of cell culture Message-ID: <5.2.1.1.2.20061018073809.00c30740@email.med.yale.edu> Does anyone have any experience with labeling of mineralization in cell cultures using fluorochrome labels (alizarin red/calcein, etc). If so, what concentration do you dose the cells with? Any suggestions? Thanks From mparker <@t> epl-inc.com Wed Oct 18 08:32:55 2006 From: mparker <@t> epl-inc.com (Mary Parker) Date: Wed Oct 18 08:33:06 2006 Subject: [Histonet] Coverslipping Film Message-ID: Does anyone have any information on repairing film coverslips. We have an Archive facility with slides that were prepared years ago and had the film cover strips on them. The problem is that the film is lifting away from the slide, and in some instances the tissue section is adhered to the film. If anyone knows how to go about repairing these slides, I would be most appreciative of any information that you might have( other than Scotch Tape) Mary D. Parker Histology Lab Manager EPL, Inc. PO Box 12766 RTP, N.C. 27709 919 998-9407 From Charlene.Henry <@t> STJUDE.ORG Wed Oct 18 08:59:38 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Oct 18 08:59:54 2006 Subject: [Histonet] Dako Eridan. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1E79@SJMEMXMB02.stjude.sjcrh.local> I would also be interested in any feed-back on the BondMax. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Bishop Sent: Tuesday, October 17, 2006 10:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Eridan. . Are there any users of the new eridan immuno stainer out there in histo land? We are looking at either the BondMax or the Eridan and personally much prefer the idea of the BondMax as have used one compared to the Eridan where they are saying there will be ONE in Australasia by the end of the year - maybe, and if we want one before 2008 we have to say now and order one now even without seeing the machine as they are only selling two to New Zealand next year! Also, are the general experiences with DAKO getting any better at the moment? So if anyone does have some feedback or any other comments to add I would like to hear them. Cheers and best wishes Alan Bishop Charge scientist Histology Medlab Central Palmerston North Tel: 06 952 3135 Fax: 06 952 3199 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lelpers <@t> bioanalytical.com Wed Oct 18 09:46:04 2006 From: lelpers <@t> bioanalytical.com (LaDonna G. Elpers) Date: Wed Oct 18 09:46:34 2006 Subject: [Histonet] Coverslipping Film Message-ID: <3100BDBD96C0724D9CE9E476BFB57D962DD1B6@basi10003.BIOANALYTICAL.COM> Hi Mary, There were issues with this a couple years ago, many comments on the Histonet. If you go to the Histonet web page www.histonet.org look to the right hand side in the black area and click on Histonet Text Archive. When the screen pops up type in the field "Tape Coverslipping" and all articles pertaining to this will be posted. There were some solutions offered but even more importantly contacts you may be able to reach to see how this was resolved for their companies. Good Luck Mary! LaDonna G. Elpers, BA, HT(ASCP) Manager, Histology BASi (Bioanalytical Systems, Inc.) 10424 Middle Mount Vernon Rd - Mount Vernon, IN 47620 P 812.985.5900 ext 128 F 812.985.3403 lelpers@bioanalytical.com www.bioanalytical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Parker Sent: Wednesday, October 18, 2006 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipping Film Does anyone have any information on repairing film coverslips. We have an Archive facility with slides that were prepared years ago and had the film cover strips on them. The problem is that the film is lifting away from the slide, and in some instances the tissue section is adhered to the film. If anyone knows how to go about repairing these slides, I would be most appreciative of any information that you might have( other than Scotch Tape) Mary D. Parker Histology Lab Manager EPL, Inc. PO Box 12766 RTP, N.C. 27709 919 998-9407 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dpconsult <@t> earthlink.net Wed Oct 18 10:31:21 2006 From: dpconsult <@t> earthlink.net (Dick Paulson [Source Medical Products]) Date: Wed Oct 18 10:31:59 2006 Subject: [Histonet] Coverslipping Film Message-ID: Mary - this is some advice from the Fred Underwood on the Histonet from 2004! Immerse the slide in acetone for about 5 minutes. The film will swell up and come right off the slide. Swish the slide in alcohol then back into xylene(a couple of changes) and coverslip. Dick Paulson From llewllew <@t> shaw.ca Wed Oct 18 11:00:41 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Oct 18 11:01:58 2006 Subject: [Histonet] Benzoyl peroxide References: <84DA9D8AC9B05F4B889E7C70238CB45104C52A82@rie2ksrv1.ri.bbsrc.ac.uk> Message-ID: <000601c6f2ce$902a16a0$130e4246@yourlk4rlmsu> After adding the benzoyl peroxide to the methacrylate, but before adding the hardener, add some anhydrous calcium chloride and shake well. After a while, the calcium chloride settles and the dry methacrylate can be decanted. Bryan Llewellyn ----- Original Message ----- From: "matt prideaux (RI)" To: Sent: Wednesday, October 18, 2006 2:12 AM Subject: [Histonet] Benzoyl peroxide Hi, I'm trying to embed some mouse tibiae in methyl methacrylate, but I'm having no luck in getting the resin to polymerise. After doing some reading I've discovered that it's probably because I didn't dry the benzoyl peroxide first (the stuff we use contains 30% water). Does anyone know of a safe way to dry the benzoyl peroxide? (I've heard lots of horror stories about ovens and explosions!) we don't have a dessicator here but there is a vacuum oven and a freeze drier, would these be ok to use? Thanks Matt The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fawn <@t> cs.cmu.edu Wed Oct 18 11:42:23 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Wed Oct 18 11:42:12 2006 Subject: [Histonet] EDTA decalcification solution Message-ID: <4536596F.80800@cs.cmu.edu> Hi everybody, I am getting ready to decalcify some mouse femurs for immuno staining. I know I have to use an EDTA decalcifier, but I do not know how to make it. Does anybody have a protocol on this that they would be willing to share? Thanks in advance Fawn From histosci <@t> shentel.net Wed Oct 18 11:50:26 2006 From: histosci <@t> shentel.net (HSRL) Date: Wed Oct 18 11:51:19 2006 Subject: [Histonet] Coverslipping Film In-Reply-To: Message-ID: <000001c6f2d5$860d1d00$0700a8c0@HSRLMAIN> Mary, We encountered the same problem years ago. We fixed tens of thousands of slides and in most cases we were able to save most of the tissue. As you can imagine, this was very tedious and time consuming. If you would like specifics on how to do this, please feel free to call 540.477.4441 and ask to speak with Ed. He will be able to give you the details and share his technique with you. Good day, Tom Tom Galati Laboratory Director HSRL, Inc.- A GLP Histopathology Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Parker Sent: Wednesday, October 18, 2006 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipping Film Does anyone have any information on repairing film coverslips. We have an Archive facility with slides that were prepared years ago and had the film cover strips on them. The problem is that the film is lifting away from the slide, and in some instances the tissue section is adhered to the film. If anyone knows how to go about repairing these slides, I would be most appreciative of any information that you might have( other than Scotch Tape) Mary D. Parker Histology Lab Manager EPL, Inc. PO Box 12766 RTP, N.C. 27709 919 998-9407 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Wed Oct 18 11:55:33 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Oct 18 11:55:42 2006 Subject: [Histonet] EDTA decalcification solution. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1E7D@SJMEMXMB02.stjude.sjcrh.local> You can make a 10% EDTA solution and adjust the pH to 7.4 but if the mouse femurs have been fixed in 10% formalin, I would suggest that you use 10% formalin saturated with EDTA. I have used both and the morphology of the bones decalcified with EDTA/Formalin is much better than the 10% solution of EDTA. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Jones Sent: Wednesday, October 18, 2006 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution. . Hi everybody, I am getting ready to decalcify some mouse femurs for immuno staining. I know I have to use an EDTA decalcifier, but I do not know how to make it. Does anybody have a protocol on this that they would be willing to share? Thanks in advance Fawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy.troiano <@t> yale.edu Wed Oct 18 12:19:32 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Wed Oct 18 12:19:47 2006 Subject: [Histonet] benzoyl peroxide mouse tibias Message-ID: <5.2.1.1.2.20061018131646.00c340d0@email.med.yale.edu> We don't dry our peroxide - that can be dangerous - instead we remove the water from the MMA/DP/BP mix by filtering through Calcium Chloride beads after dissolving the benzoyl peroxide in the methacrylate. Also, we polymerize our mouse tibias in a 37 deg C radiant heat oven in a waterbath - you can look up our methods in the Journal of Histotechnology, , Vol. 27, No. 2, PP119-130. From pathderm <@t> yahoo.com Wed Oct 18 12:45:10 2006 From: pathderm <@t> yahoo.com (bobby king) Date: Wed Oct 18 12:45:22 2006 Subject: [Histonet] Histology School Message-ID: <20061018174510.29379.qmail@web50010.mail.yahoo.com> With the number of Histology Schools decreasing and the critical shortage of qualified Histologists, we are considering establishing a training program to provide the academic as well as the practical day to day applications necessary for anyone interested in becoming a Histologist. I would appreciate any feedback from anyone who has considered this option or is presently in a Histology program. Robert King, BS, MA Laboratory Services Director From TJJ <@t> Stowers-Institute.org Wed Oct 18 13:25:52 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Oct 18 13:26:28 2006 Subject: [Histonet] Re: EDTA decalcification solution Message-ID: Fawn, If you do a histonet search (www.histosearch.com/histonet) for EDTA decalcification, you will pull up a lot of useful information. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Margaret.Perry <@t> sdstate.edu Wed Oct 18 13:50:09 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Oct 18 13:50:18 2006 Subject: [Histonet] CAP and thermometers Message-ID: We are moving towards accreditation by AAVLD(veterinary) which will be similar to CAP. I am wondering how you calibrate thermometers for use in the slide drying oven. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From dfinkelstein <@t> mhri.edu.au Wed Oct 18 13:41:57 2006 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Wed Oct 18 14:13:26 2006 Subject: [Histonet] RE: Controls for nickel enhanced DAB Message-ID: <000f01c6f2e5$176845e0$0200a8c0@davidfink> Hi Bob, Cobalt Nickel DAB is my favourite was of intensifying IHC [2]. This is then counter stained with Neutral red to give good quality images. Leaving out the primary antibody is often an adequate control especially if you can identify the antigen in at a specific location in the brain. "I'm staining for receptors in mouse CNS, and don't see any staining in control sections and some diffuse staining of varying intensity in the experimental tissue." It sounds like you have to try playing with the level of fixation. Receptors are very difficult to visualise and in my previous studies with the Dopamine transporter fixation is crucial [1]. It is very difficult to design experiments with quantitative densotometric IHC analysis whereas it is, it is relatively easy to do stereological studies 1. Parish, C.L., D.I. Finkelstein, J. Drago, E. Borrelli, and M.K. Horne, The role of dopamine receptors in regulating the size of axonal arbors. Journal of Neuroscience, 2001. 21(14): p. 5147-57. 2. Adams, J.C., Heavy metal intensification of DAB-based HRP reaction product. Journal of Histochemistry and Cytochemistry, Journal of Histochemistry and Cytochemistry, 1981. 29: p. 775. Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA Mobile: 0409171227 Tel: +61 (03) 9388 1633 Fax: +61 (03) 9387 5061 dfinkelstein@mhri.edu.au d From Maxim_71 <@t> mail.ru Wed Oct 18 14:20:51 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Wed Oct 18 14:22:34 2006 Subject: [Histonet] from Maxim Message-ID: <8212423375.20061018232051@mail.ru> Dear Rene! 1- I want once again thank to you for PT/PM Hematoxyline protocol. Today I was received zinc chloride and stained slides with this procedure. These results much better than with bichromat acetic acid, which I use earlier. Old procedure require 48 hours, but new (your protocol) less than 2 hours. 2- Also I was receive Gallocyanin and will be able use the methods, include this dye. But, on a label not contain C.I.?, and its molecular formula C15H12N2O5 (instead C15H13N2O5Cl, as mentioned B. Llewellin on this site). I cannot have other source for info about dyes. Our gallocyanin manufactured maybe in Russia (no dates on label). 3- I was tried ice cubes for cooling embedded block immeditealy afterwards, but advantage when cutting has not noticed. If the blocks immediately after embedding place into freezer, then blocks will be cracked. Thereby too strong difference I have not noticed. Maybe I do something wrong? 4- Some pathologists, HTs and medical technologist read your article ("Histology: a unique area of the Medical Laboratory.") with great taste and these paper was very interesting and useful for they. They are send to you separate thanks. I very pleased was once again you to write. Best regards from all. Sincerely, Maxim. From robert.bates <@t> eglin.af.mil Wed Oct 18 14:23:56 2006 From: robert.bates <@t> eglin.af.mil (Bates Robert R Maj MIL USAF 96 MDSS/SGSAH) Date: Wed Oct 18 14:24:47 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: <29E7697C9175724D921A904EB1DAEEDB01095E37@mail06.eglin.af.mil> Hello, We have a Ventana Benchmark that has been giving my histotechs fits. There are times when the staining is hit or miss. Other times the controls will not stain properly but the specimen tissue does or visa versa. We have troubleshot the machine numerous times without success. We call the aid line and do the things they recommend and it 'may' work for a while and then it reverts back to the hit or miss schedule. We have tried random tissue sections that have know positive properties (such as smooth muscle tissue stained with smooth muscle actin) and even observed internal tissue controls to see if they react properly. Has anyone else had this type of problem with this machine? Any ideas or suggestions would be most welcome. Dr. Robert R. Bates Jr Florida From rjbuesa <@t> yahoo.com Wed Oct 18 14:46:02 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 18 14:46:08 2006 Subject: [Histonet] EDTA decalcification solution In-Reply-To: <4536596F.80800@cs.cmu.edu> Message-ID: <20061018194602.15976.qmail@web61214.mail.yahoo.com> Fawn: This is how I used to prepare it: EDTA --- 250g Dist. water ---1,750 mL Add the EDTA to the water in a hot plate with constant agitation (teflon covered magnet). When all the salt is dissolved the solution will have a "milky" appearance. Leave the solution reach room temperature and determine pH (ideally with a pH-meter). The solution will be acid ( wrote: Hi everybody, I am getting ready to decalcify some mouse femurs for immuno staining. I know I have to use an EDTA decalcifier, but I do not know how to make it. Does anybody have a protocol on this that they would be willing to share? Thanks in advance Fawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From doug <@t> ppspath.com Wed Oct 18 14:49:43 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Oct 18 14:47:08 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: <29E7697C9175724D921A904EB1DAEEDB01095E37@mail06.eglin.af.mil> Message-ID: It could be a vortex mixer issue. Call the help line and mention this to them. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bates Robert R Maj MIL USAF 96 MDSS/SGSAH Sent: Wednesday, October 18, 2006 3:24 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana Benchmark Problems Hello, We have a Ventana Benchmark that has been giving my histotechs fits. There are times when the staining is hit or miss. Other times the controls will not stain properly but the specimen tissue does or visa versa. We have troubleshot the machine numerous times without success. We call the aid line and do the things they recommend and it 'may' work for a while and then it reverts back to the hit or miss schedule. We have tried random tissue sections that have know positive properties (such as smooth muscle tissue stained with smooth muscle actin) and even observed internal tissue controls to see if they react properly. Has anyone else had this type of problem with this machine? Any ideas or suggestions would be most welcome. Dr. Robert R. Bates Jr Florida _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 18 14:50:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 18 14:50:55 2006 Subject: [Histonet] CAP and thermometers In-Reply-To: Message-ID: <20061018195052.76242.qmail@web61218.mail.yahoo.com> Margaret: I used to run a series of temperature readings with my thermometers against one certified by the National Bureau of Standards. Average and standard deviations of the readings to find out if there was accordance with the calibrated thermometer. Ren? J. "Perry, Margaret" wrote: We are moving towards accreditation by AAVLD(veterinary) which will be similar to CAP. I am wondering how you calibrate thermometers for use in the slide drying oven. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From LuckG <@t> empirehealth.org Wed Oct 18 15:09:13 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Oct 18 15:09:22 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: <6BB8BC4519AAB844B174FC739A679BBC50905A@IRMEXCH01.irm.inhs.org> Dr. Bates, Be thankful you're not trying to get tech support on one of Ventana's tissue processors. Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bates Robert R Maj MIL USAF 96 MDSS/SGSAH Sent: Wednesday, October 18, 2006 12:24 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana Benchmark Problems Hello, We have a Ventana Benchmark that has been giving my histotechs fits. There are times when the staining is hit or miss. Other times the controls will not stain properly but the specimen tissue does or visa versa. We have troubleshot the machine numerous times without success. We call the aid line and do the things they recommend and it 'may' work for a while and then it reverts back to the hit or miss schedule. We have tried random tissue sections that have know positive properties (such as smooth muscle tissue stained with smooth muscle actin) and even observed internal tissue controls to see if they react properly. Has anyone else had this type of problem with this machine? Any ideas or suggestions would be most welcome. Dr. Robert R. Bates Jr Florida _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Wed Oct 18 15:33:36 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Oct 18 15:29:23 2006 Subject: [Histonet] (no subject) Message-ID: <63B8B599DE283148B92E83C78B32C15D032580C3@cmhexbe2.childrensmemorial.org> Hi histoneters: My question probably very stupid :( I did some immunoperoxide staining with full protocol (blocking steps, Ab primary and secondary, tertiary, DAB, hematoxylin counterstained, alcohols, xylin and coverslipped. My question is: Is it possible to reuse/restain with other Ab this slide again? Thanks in advance, Naira From portera <@t> msu.edu Wed Oct 18 15:30:40 2006 From: portera <@t> msu.edu (Amy Porter) Date: Wed Oct 18 15:29:51 2006 Subject: [Histonet] Re: EDTA decalcification solution References: Message-ID: <001501c6f2f4$46d4c8d0$8e7a0923@HistoJJ> I was able to obtain a lot of useful information on EDTA decalcification just recently from histonet. Anything that was discussed would be in the archives from just this month. ----- Original Message ----- From: "Johnson, Teri" To: Sent: Wednesday, October 18, 2006 2:25 PM Subject: [Histonet] Re: EDTA decalcification solution Fawn, If you do a histonet search (www.histosearch.com/histonet) for EDTA decalcification, you will pull up a lot of useful information. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From belinda_cairns <@t> yahoo.com Wed Oct 18 17:43:35 2006 From: belinda_cairns <@t> yahoo.com (Belinda Cairns) Date: Wed Oct 18 17:43:39 2006 Subject: [Histonet] Frozen Tissue Sections Message-ID: <20061018224335.49775.qmail@web50403.mail.yahoo.com> I have a very quick question I hope! Does anyone know where I can purchase frozen sections on slides commercially? I need some frozen sections (any tissues, normal or diseased) ready for IHC in an independant lab with no access to tissues or ways to section tissues. We are a chemistry lab working on new dyes for IHC and instrumentation detection. I can get paraffin sections commercially but I would prefer frozen as I am looking at new fluorescent dyes. Thanks in advance Belinda Cairns __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From AnthonyH <@t> chw.edu.au Wed Oct 18 17:50:58 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Oct 18 17:52:34 2006 Subject: [Histonet] EDTA decalcification solution Message-ID: Try this: EDTA Decalcification Mixtures Ethylenediaminetetraacetic Acid (EDTA) is a calcium absorbent that has gained popularity because of its gentleness on tissue and antigens. It is a slow decalcifier but the rate of decalcification can be increased with use of microwave energy (Sanderson et al 1995 Biotech & Histochem 70(1):12-18): 1. To 1 litre of distilled water add 90ml concentrated ammonium hydroxide. 2. Stirring continuously, slowly add 140g EDTA 3. Adjust to pH 7.1 using concentrated ammonia. Adjustment of the pH is essential. If the pH is too low it works only as a too-weak acid. If the pH is too high (above 8) decalcification is accelerated but alkalinity can be damaging to tissues. EDTA has also been combined with Hydrochloric acid resulting in a rapid decalcifier. In this case it seems to act by combining with the calcium as it is solubilized by the acid. This allows the newly freed acid to remove more calcium: Dissolve 20g EDTA (di-sodium salt) in 1860ml of distilled water, and then add 140ml concentrated hydrochloric acid. A precipitate may form within 24 hours of preparation of the solution but this does not appear to affect the usefulness of the solution. Since EDTA will precipitate in alcohols, it is important to wash tissues in water. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Jones Sent: Thursday, 19 October 2006 2:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution Hi everybody, I am getting ready to decalcify some mouse femurs for immuno staining. I know I have to use an EDTA decalcifier, but I do not know how to make it. Does anybody have a protocol on this that they would be willing to share? Thanks in advance Fawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From anh2006 <@t> med.cornell.edu Wed Oct 18 17:57:21 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Oct 18 17:57:53 2006 Subject: [Histonet] Frozen Tissue Sections In-Reply-To: <20061018224335.49775.qmail@web50403.mail.yahoo.com> References: <20061018224335.49775.qmail@web50403.mail.yahoo.com> Message-ID: Pathology Associates International in Maryland is where I have purchased them from in the past. They aren't cheap though and there may be a more inexpensive place. Also I purchased from frozen arrays from Tristar. LMK if you want contact info for either place. >I have a very quick question I hope! Does anyone know >where I can purchase frozen sections on slides >commercially? I need some frozen sections (any >tissues, normal or diseased) ready for IHC in an >independant lab with no access to tissues or ways to >section tissues. We are a chemistry lab working on >new dyes for IHC and instrumentation detection. I can >get paraffin sections commercially but I would prefer >frozen as I am looking at new fluorescent dyes. > >Thanks in advance > >Belinda Cairns -- From jnocito <@t> satx.rr.com Wed Oct 18 18:48:11 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Oct 18 18:48:26 2006 Subject: [Histonet] Ventana Benchmark Problems References: <29E7697C9175724D921A904EB1DAEEDB01095E37@mail06.eglin.af.mil> Message-ID: <007801c6f30f$e2c3a750$066ace44@yourxhtr8hvc4p> yes and it's not even Friday yet. Hey Doc, we have 3 of these "great" machines and it drives us nuts also. Make it a point, in fact, demand that an engineering rep come out. We have had items replaced such as the vortex mixer, heating pads, the entire carousel many times, yet for some strange reason, we keep getting more and more. The big thing now is we switched to their polymer detection kit and you talk about fits. All our slides have a hit and miss on staining endogenous peroxidase. Our rabbit and mouse negative controls stain as brightly as our primaries. Yes, we had two "wiz" kids out and I was not impressed. A senior specialist is coming to my lab to figure out the problem. You see me holding my breath. I told the group not to go ahead with the Ventana machines, but they blew me off. Said I had an attitude problem because I was screwed over when I was at Wilford Hall. Gee, maybe I was on to something. Now, we are in so deep we'll never get out. If it was my lab, I would have went with Biocare's Nemesis, but like I said, it's not my lab. If you can change machines, I would. Save yourself a lot of headache and money. Now, I expect all the Ventana reps and their lawyers to contact my CEO tomorrow. Go ahead, make my day. Save yourself the phone call. Whatever I said here, I will say in court too. Of course, the opinion expressed by me is not the opinion of my pathologists, CEO, their lawyers, their wives, off spring, their off spring and their dogs and cats. Joe Nocito BS, PA, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Bates Robert R Maj MIL USAF 96 MDSS/SGSAH" To: Sent: Wednesday, October 18, 2006 2:23 PM Subject: [Histonet] Ventana Benchmark Problems > Hello, > > > > We have a Ventana Benchmark that has been giving my histotechs fits. > There > are times when the staining is hit or miss. Other times the controls will > not stain properly but the specimen tissue does or visa versa. We have > troubleshot the machine numerous times without success. We call the aid > line and do the things they recommend and it 'may' work for a while and > then > it reverts back to the hit or miss schedule. We have tried random tissue > sections that have know positive properties (such as smooth muscle tissue > stained with smooth muscle actin) and even observed internal tissue > controls > to see if they react properly. Has anyone else had this type of problem > with this machine? Any ideas or suggestions would be most welcome. > > > > Dr. Robert R. Bates Jr > > Florida > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dahui_you <@t> yahoo.com Wed Oct 18 19:08:21 2006 From: dahui_you <@t> yahoo.com (Dahui You) Date: Wed Oct 18 19:08:25 2006 Subject: [Histonet] cytospin slide staining Message-ID: <20061019000821.48270.qmail@web35706.mail.mud.yahoo.com> Hello, histonetters: Has anyone done cytospin slide staining before? What antigen retrieval method you used? I am staining bronchoalveolar lavage fluid slides with anti-active caspase3 and none of my experimental and control slides showed positive. I am wondering if that is real or I did it wrong since I don't have any positive control slides for this antibody and I never did this kind of staining before. Any suggestion will be greatly appreciated! Have a nice day. Dahui You LSU From mtarango <@t> nvcancer.org Wed Oct 18 19:31:36 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Oct 18 19:32:03 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1CEFC@NVCIEXCH02.NVCI.org> I'm with you on this one! The kits and reagents are very expensive and the hit and miss staining drives me nuts. I have to re-stain the same slide over and over again because the control stained and the patient tissue didn't have any reactive elements or there was no staining at all. I also can't stand that the instrument won't stain slides because I run out of something like EZprep, which just dewaxes the slide. If I run a slide down to water and put it on the instrument why should it need EZrep? I mean, there is no paraffin on the slide and the protocol has been modified accordingly, so I think the stupid thing should run. Another thing, what is the deal with the expiration dates? Their kits are the most expensive and expire the soonest. After they expire, they're dead. Not even for research purposes or if there were an emergency could you get the machine to run with an expired kit. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, October 18, 2006 4:48 PM To: Bates Robert R Maj MIL USAF 96 MDSS/SGSAH; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Benchmark Problems yes and it's not even Friday yet. Hey Doc, we have 3 of these "great" machines and it drives us nuts also. Make it a point, in fact, demand that an engineering rep come out. We have had items replaced such as the vortex mixer, heating pads, the entire carousel many times, yet for some strange reason, we keep getting more and more. The big thing now is we switched to their polymer detection kit and you talk about fits. All our slides have a hit and miss on staining endogenous peroxidase. Our rabbit and mouse negative controls stain as brightly as our primaries. Yes, we had two "wiz" kids out and I was not impressed. A senior specialist is coming to my lab to figure out the problem. You see me holding my breath. I told the group not to go ahead with the Ventana machines, but they blew me off. Said I had an attitude problem because I was screwed over when I was at Wilford Hall. Gee, maybe I was on to something. Now, we are in so deep we'll never get out. If it was my lab, I would have went with Biocare's Nemesis, but like I said, it's not my lab. If you can change machines, I would. Save yourself a lot of headache and money. Now, I expect all the Ventana reps and their lawyers to contact my CEO tomorrow. Go ahead, make my day. Save yourself the phone call. Whatever I said here, I will say in court too. Of course, the opinion expressed by me is not the opinion of my pathologists, CEO, their lawyers, their wives, off spring, their off spring and their dogs and cats. Joe Nocito BS, PA, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Bates Robert R Maj MIL USAF 96 MDSS/SGSAH" To: Sent: Wednesday, October 18, 2006 2:23 PM Subject: [Histonet] Ventana Benchmark Problems > Hello, > > > > We have a Ventana Benchmark that has been giving my histotechs fits. > There > are times when the staining is hit or miss. Other times the controls will > not stain properly but the specimen tissue does or visa versa. We have > troubleshot the machine numerous times without success. We call the aid > line and do the things they recommend and it 'may' work for a while and > then > it reverts back to the hit or miss schedule. We have tried random tissue > sections that have know positive properties (such as smooth muscle tissue > stained with smooth muscle actin) and even observed internal tissue > controls > to see if they react properly. Has anyone else had this type of problem > with this machine? Any ideas or suggestions would be most welcome. > > > > Dr. Robert R. Bates Jr > > Florida > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From tncperfect2 <@t> comcast.net Wed Oct 18 20:21:15 2006 From: tncperfect2 <@t> comcast.net (tncperfect2@comcast.net) Date: Wed Oct 18 20:21:20 2006 Subject: [Histonet] Shandon Path Center Message-ID: <101920060121.9834.4536D30B0001C28C0000266A2200751090CD9B0C0A009D0A9F0C029B@comcast.net> Hi Histonetters, I have a Shandon Path Center Processor. Does anyone have any extra caps from the bottles they would be willing to let go of. I have a couple of broken caps but to get just a couple of caps I have to purchase the whole bottle. If anyone has a Shandon Path Center Processor hanging around in their "used" warehouse or storage room I would appreciate the help. Thanks, Carolyn From BMolinari <@t> heart.thi.tmc.edu Thu Oct 19 06:19:27 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 19 06:19:32 2006 Subject: [Histonet] EDTA decalcification solution In-Reply-To: <4536596F.80800@cs.cmu.edu> Message-ID: Hi Fawn, I use a product called Versenate Decal Solution from American Master Tech Scientific (1-800-860-4073) Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Jones Sent: Wednesday, October 18, 2006 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution Hi everybody, I am getting ready to decalcify some mouse femurs for immuno staining. I know I have to use an EDTA decalcifier, but I do not know how to make it. Does anybody have a protocol on this that they would be willing to share? Thanks in advance Fawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Thu Oct 19 07:32:04 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Oct 19 07:33:44 2006 Subject: [Histonet] cytospin slide staining In-Reply-To: <20061019000821.48270.qmail@web35706.mail.mud.yahoo.com> Message-ID: GEAUX TIGERS!!! I'm presuming your cytospins are fixed in 95% Alcohol? Sometimes antibodies needing antigen retrieval for formalin fixed paraffin embedded slides will not need any antigen retrieval, or will need an entirely different protocol. Its often hit and miss trials. I've found that exposure to methanol is the kiss of death for many antibodies that will work with ethanol fixed protocols. If you are working in human tissues, you can make a decent control by making a cell suspension from minced skin, but of course, best of all would be cervical cancer cells, suspended in RPMI, and make your cytospin controls. From a proud LSU alumnus Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dahui You Sent: Wednesday, October 18, 2006 8:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytospin slide staining Hello, histonetters: Has anyone done cytospin slide staining before? What antigen retrieval method you used? I am staining bronchoalveolar lavage fluid slides with anti-active caspase3 and none of my experimental and control slides showed positive. I am wondering if that is real or I did it wrong since I don't have any positive control slides for this antibody and I never did this kind of staining before. Any suggestion will be greatly appreciated! Have a nice day. Dahui You LSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From DBotsfor <@t> wrh.on.ca Thu Oct 19 07:31:11 2006 From: DBotsfor <@t> wrh.on.ca (Botsford, Daniel) Date: Thu Oct 19 07:35:51 2006 Subject: [Histonet] Artifacts- condensate like Message-ID: <331EE6D48DDFD51189E500508BBD360806D98453@mail.wrh.on.ca> Hello... Is there a remedy for condensate artifact? There is complete lost of nuclear detail in the cell. The nucleus stains homogenous light blue or white. This artifact was generated in prostate biopsies after a machine malfunction. Tissue processer reagents leaked into the waxes. I have seen the condensate artifact in GI biopsies before if the alcohol are not changed frequently and the absolute alcohol becomes contaminated with water and carrys over to the xylol. I have heard of it occurring on weekend runs when the formalin condenses on the lid of the retort and drops back into nonaqueous solutions during processing. I have never heard of a remedy. Sincerely Daniel Botsford Windsor Regional Hospital 1995 Lens Avenue Windsor, Ontario N8W 1L9 519-254-5577 ext 52373 519-254-6861 fax dbotsfor@wrh.on.ca From Rcartun <@t> harthosp.org Thu Oct 19 08:04:47 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Oct 19 08:05:42 2006 Subject: [Histonet] Dako Eridan In-Reply-To: References: Message-ID: <45373FAF0200007700002705@hcnwgwds01.hh.chs> I had the opportunity to work with the Vison BioSystems "Bond-Max" last week at their facility in Norwell, MA. I hadn't set-up IHC slides for a few years so it was a lot fun for me to get back into the lab and get my hands wet. I set up approximately 100 IP stains (mostly CD markers), as well as several in situ hybridization slides. The quality and uniformity of the immunoreactivity (and hybridization) was "outstanding". I have never seen such beautiful slides before. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Alan Bishop" 10/17/06 11:57 PM >>> Are there any users of the new eridan immuno stainer out there in histo land? We are looking at either the BondMax or the Eridan and personally much prefer the idea of the BondMax as have used one compared to the Eridan where they are saying there will be ONE in Australasia by the end of the year - maybe, and if we want one before 2008 we have to say now and order one now even without seeing the machine as they are only selling two to New Zealand next year! Also, are the general experiences with DAKO getting any better at the moment? So if anyone does have some feedback or any other comments to add I would like to hear them. Cheers and best wishes Alan Bishop Charge scientist Histology Medlab Central Palmerston North Tel: 06 952 3135 Fax: 06 952 3199 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eva.alstromer <@t> histolab.se Thu Oct 19 08:41:35 2006 From: eva.alstromer <@t> histolab.se (=?iso-8859-1?Q?Eva_Alstr=F6mer?=) Date: Thu Oct 19 08:41:54 2006 Subject: [Histonet] unsubscribe Message-ID: <000001c6f384$4c0fe2e0$65b6a8c0@HISSWE.lokal> Eva Alstr?mer Histolab Products AB S?dra L?ngebergsgatan 34 S-421 32 V Fr?lunda Sweden +46 (31) 7093032 eva.alstromer@histolab.se www.histolab.se From joost.bruijntjes <@t> tno.nl Thu Oct 19 08:55:45 2006 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Thu Oct 19 08:56:14 2006 Subject: [Histonet] cytospin slide staining Message-ID: <1A7F09509ACB294B835A94B2C8EB50F4023E48D0@MS-DT01VS01.tsn.tno.nl> Hi I've done anti cleaved caspase on ethanol (70%) fixed tissues and I used an EDTA-buffer pH 8.0 with good results. The first time I used this antibody I've tried the citrate buffer pH 6.0 with no results at all, but changing to pH 8.0 gave me better results. Joost Bruijntjes TNO Quality of Life Tel: +31 30 6944480 Zeist Holland -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dahui You Sent: Wednesday, October 18, 2006 8:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytospin slide staining Hello, histonetters: Has anyone done cytospin slide staining before? What antigen retrieval method you used? I am staining bronchoalveolar lavage fluid slides with anti-active caspase3 and none of my experimental and control slides showed positive. I am wondering if that is real or I did it wrong since I don't have any positive control slides for this antibody and I never did this kind of staining before. Any suggestion will be greatly appreciated! Have a nice day. Dahui You LSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From naje1972 <@t> yahoo.com Thu Oct 19 09:05:17 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Thu Oct 19 09:05:31 2006 Subject: [Histonet] Immunohistochemical stainers Message-ID: <20061019140517.16430.qmail@web33011.mail.mud.yahoo.com> Dear Histonetter I,d like to add my 2 cents to this issue. We are stuck with a lemon of a stainer also, I refuse to use the thing. It also is hit and miss. It's a great idea these machines, but the companies that are pushing this machine need to work out the bugs and then if there is a problem please give us good customer service, with knowlegdable people who having a working knowlegde of these contraptions,as well as the methodalogy of the staining techniques. I know how to trouble shoot a lot of the problems that usually acompany's immunohistochemistry but with these machines I am totally at the mercy of some rep who may know or may not know. So there I've aired my complaint. Everyone have a GOOD DAY. Thank you, Cynthia Haynes H.T. From matt.prideaux <@t> bbsrc.ac.uk Thu Oct 19 09:39:35 2006 From: matt.prideaux <@t> bbsrc.ac.uk (matt prideaux (RI)) Date: Thu Oct 19 09:39:53 2006 Subject: [Histonet] Re: Benzoyl peroxide Message-ID: <84DA9D8AC9B05F4B889E7C70238CB45104C52A88@rie2ksrv1.ri.bbsrc.ac.uk> Hi Everyone, Thanks for all your help and advice. I've decided not to dry the benzoyl peroxide (sounds too dangerous and understandably the H&S officer isn't too keen), I'll remove it from the MM/DBP/BP mixture as advised. Hopefully this won't be a problem in the future as I'm going to use LR White instead. I've used it in the past and it seems so much easier. Cheers, Matt The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute. From George.Adams <@t> UTSouthwestern.edu Thu Oct 19 10:00:32 2006 From: George.Adams <@t> UTSouthwestern.edu (George Adams) Date: Thu Oct 19 10:00:51 2006 Subject: [Histonet] Safranin-O Message-ID: <45374CC002000018000081E7@swnw126.swmed.edu> HISTONETTERS: I am trying to find out a detailed comparison of Safranin-O versus Alcian blue stains for glycosaminoglycans and proteoglycans. Specifically, does Safranin-O stain sulfated (chondroitin sulfate) or only non-sulfated GAGs (hyaluronic acid)? Is there an advantage or disadvantage to using Alcian blue for the same glycosaminoglycans? The tissue that one of the students in our lab will be using is from human umbilical cord. I received a very nice and quick response from Mr. Charles Churukian via Mr. Matthew Frank at the the University of Rochester Medical Center which is in bold below. Mr. Adams, I was just informed by Charles Churukian that Alcian Blue is the correct stain to use for an umbilical cord section. Safranin O has been used for bone tissue but for this application Alcain Blue is your stain. I have no info on comparison studies between the two. Matthew Frank Looking forward to everyone's responses. George Adams, Ph.D. Research Associate University of Texas Southwestern Medical Center Department of Otolaryngology 5323 Harry Hines Blvd. Dallas TX 75230-5433 214-648-0466 From PIXLEYSK <@t> UCMAIL.UC.EDU Thu Oct 19 10:02:19 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Thu Oct 19 10:02:03 2006 Subject: [Histonet] Controls for Nickel enhanced DAB Message-ID: Dear Bob Nienhuis: My preferred control (somewhat old fashioned and hard core now) for IHC and antibody experiments is to use a solution that is as close to your primary antibody as possible. So if your primary antibody is unprocessed rabbit serum, then normal rabbit serum is your best control. Sera vary greatly, especially in their non-specific staining. So of course the optimal control solution is the pre-immune sera from the animal that you got your primary from, taken prior to injection of the antigen!! But today it is extremely rare to be able to have that. So here are two other ways to ensure a good control. First, do a protein determination on both sera and make sure that you are using at least the same protein concentration (or more) than you have in your primary. Second, some rabbit sera just have endogenous background binding. I had to throw one out one year because it was so much higher in background than my primary antibody. You may have to stain and try some to find one that does not give too high a background. Is this completely kosher? I rationalized it because the great variation between animal sera is natural and some sera just do give background. If your primary antibody is either a mouse monoclonal or a processed primary antibody, then the place that you obtained your antibody can help you find a good control. For the mouse monoclonal, ATCC often sells specific antibodies or solutions that serve as controls for their primaries. These might be, for example, culture supernatants from a non-antibody producing cell line that was grown with the same culture supernatant, and collected and processed similarly. If you have a primary serum that is processed, the commercial companies will work with you to find a similarly processed solution that does not have any antibody in it that will bind to your tissue. I.e., I have used antibodies against bacterial proteins from the same company, processed in the same way. ETC. These are much better controls than anything you can come up with in your lab. Removing primary is usually deceptively clean. It does NOT provide a good background check!!!! Another important thing to do in your case is to do a dilution curve of the primary antibody. That will allow you to see if the background goes down while specific staining stays. FYI, I used to use cobalt-nickel enhancement, but it was horrible to work with. Too much precipitate. I now use a stock of 2% nickel sulfate, NiSO4.6H2O: 0.9 g into 45 ml E-pure dd H2O = 2% solution. Keep in Fridge. Dilute: 1/25 in your DAB solution to give a 0.08% solution. Seems just as sensitive, gives a good blue-black color and less precipitate in both stock and the DAB solution, although there still is some. Best wishes. Sarah Pixley From NMargaryan <@t> childrensmemorial.org Thu Oct 19 10:07:50 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Oct 19 10:03:38 2006 Subject: [Histonet] (no subject) Message-ID: <63B8B599DE283148B92E83C78B32C15D03258135@cmhexbe2.childrensmemorial.org> Hi histo-s, What is the best company to buy Hematoxylin and Eosin? Thanks, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From NMargaryan <@t> childrensmemorial.org Thu Oct 19 10:37:00 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Oct 19 10:32:40 2006 Subject: [Histonet] (no subject) Message-ID: <63B8B599DE283148B92E83C78B32C15D03258143@cmhexbe2.childrensmemorial.org> Hi histoneters again, Thanks you all for the answers. Let me clarify. I mostly use tumor tissue (melanoma, breast cancer, prostate). What the best company for Hematoxylin and Eosin you will suggest me for this purpose? Thanks, Naira From mauger <@t> email.chop.edu Thu Oct 19 10:36:11 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Thu Oct 19 10:36:49 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: To All, Beware-Ventana reps told me they were buying VisionBiosystems. I don't know how true it is, but I worry about the future of the 'Bondmax' in Ventanas hands! This is just FYI. Jo From Charlene.Henry <@t> STJUDE.ORG Thu Oct 19 10:53:42 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Thu Oct 19 10:53:54 2006 Subject: [Histonet] Ventana Benchmark Problems. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1E84@SJMEMXMB02.stjude.sjcrh.local> The bashing is brutal this morning so I might as well add my 2 cents worth. I have been using Ventana instruments for over 15 years and they have always been excellent instruments. I now have 2 Benchmark XT instruments and the slides are consistently beautiful. I have always found their tech support to be very helpful and my down-time is minimum. Are all of you using the same Ventana company that I'm using???? By the way we also have 2 Dako stainers and we do experience inconsistent staining with these instruments from time to time. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Thursday, October 19, 2006 10:36 AM To: robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu; mtarango@nvcancer.org; jnocito@satx.rr.com Subject: RE: [Histonet] Ventana Benchmark Problems. . To All, Beware-Ventana reps told me they were buying VisionBiosystems. I don't know how true it is, but I worry about the future of the 'Bondmax' in Ventanas hands! This is just FYI. Jo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Oct 19 10:46:37 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Oct 19 11:01:36 2006 Subject: [Histonet] (no subject) In-Reply-To: <63B8B599DE283148B92E83C78B32C15D03258135@cmhexbe2.childrensmemorial.org> References: <63B8B599DE283148B92E83C78B32C15D03258135@cmhexbe2.childrensmemorial.org> Message-ID: <45379DDD.5000605@umdnj.edu> As long as you buy dyes that are certified by the Biological Stain Commission you will be fine. Each bottle will have a extra lable from the BSC stating that they have tested that lot. Geoff Margaryan, Naira wrote: >Hi histo-s, > > > >What is the best company to buy Hematoxylin and Eosin? > > > >Thanks, > >Naira > > > >Naira V. Margaryan, D.V.M., Ph.D. > >Research Associate III > >Children's Memorial Research Center > >2300 Children's Plaza, Box 222 > >Chicago, IL 60614-3394 > >Tel: 773-755-6570/ext-8 > >Fax: 773-755-6594 > >nmargaryan@childrensmemorial.org > > > > >For Express Mail: > >CMRC, Room C.473 > >2430 N. Halsted Street > >Chicago, IL 60614-4314 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Thu Oct 19 11:09:39 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 19 11:09:52 2006 Subject: [Histonet] (no subject) In-Reply-To: <63B8B599DE283148B92E83C78B32C15D032580C3@cmhexbe2.childrensmemorial.org> Message-ID: <20061019160939.71709.qmail@web61215.mail.yahoo.com> Naira: Yes, you can. Hydrate the section and do the new protocol. You will not need to repeat the blocking step; start with the new antibody. Ren? J. "Margaryan, Naira" wrote: Hi histoneters: My question probably very stupid :( I did some immunoperoxide staining with full protocol (blocking steps, Ab primary and secondary, tertiary, DAB, hematoxylin counterstained, alcohols, xylin and coverslipped. My question is: Is it possible to reuse/restain with other Ab this slide again? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From rjbuesa <@t> yahoo.com Thu Oct 19 11:15:32 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 19 11:15:43 2006 Subject: [Histonet] (no subject) In-Reply-To: <63B8B599DE283148B92E83C78B32C15D03258135@cmhexbe2.childrensmemorial.org> Message-ID: <20061019161532.74043.qmail@web61219.mail.yahoo.com> In my experience: Richard- Allan Ren? J. "Margaryan, Naira" wrote: Hi histo-s, What is the best company to buy Hematoxylin and Eosin? Thanks, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From clfields12 <@t> alltel.net Thu Oct 19 11:54:42 2006 From: clfields12 <@t> alltel.net (fields) Date: Thu Oct 19 11:54:53 2006 Subject: [Histonet] Ventana Purchase Message-ID: <000601c6f39f$4637ced0$01fea8c0@LARRYCAROL> According to reliable sources this merger has fallen thru. From mcauliff <@t> umdnj.edu Thu Oct 19 10:48:10 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Oct 19 12:05:40 2006 Subject: [Histonet] Safranin-O In-Reply-To: <45374CC002000018000081E7@swnw126.swmed.edu> References: <45374CC002000018000081E7@swnw126.swmed.edu> Message-ID: <45379E3A.6030308@umdnj.edu> If such research existed Dr. Churukian would know about it. Alcian Blue has been the subjected to more research for this application than Safranin O. Geoff George Adams wrote: >HISTONETTERS: > I am trying to find out a detailed comparison of Safranin-O >versus >Alcian blue stains for glycosaminoglycans and proteoglycans. >Specifically, does Safranin-O stain sulfated (chondroitin sulfate) or >only non-sulfated GAGs (hyaluronic acid)? Is there an advantage or >disadvantage to using Alcian blue for the same glycosaminoglycans? The >tissue that >one of the students in our lab will be using is from human umbilical >cord. > I received a very nice and quick response from Mr. Charles >Churukian via Mr. Matthew Frank at the >the University of Rochester Medical Center which is in bold below. > >Mr. Adams, > >I was just informed by Charles Churukian that Alcian Blue is the >correct >stain to use for an umbilical cord section. Safranin O has been used >for bone tissue but for this application Alcain Blue is your stain. I >have no info on comparison studies between the two. > >Matthew Frank > > >Looking forward to everyone's responses. > > > George Adams, Ph.D. > > Research Associate > > University of Texas Southwestern >Medical Center > > Department of Otolaryngology > > 5323 Harry Hines Blvd. > > Dallas TX 75230-5433 > > 214-648-0466 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From lhadley <@t> iupui.edu Thu Oct 19 12:16:43 2006 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Thu Oct 19 12:16:51 2006 Subject: [Histonet] Ventana Purchase In-Reply-To: <000601c6f39f$4637ced0$01fea8c0@LARRYCAROL> Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF213FE6C3@iu-mssg-mbx105.ads.iu.edu> They're just going to sue them instead. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fields Sent: Thursday, October 19, 2006 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Purchase According to reliable sources this merger has fallen thru. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Thu Oct 19 12:20:40 2006 From: contact <@t> excaliburpathology.com (P Pierce) Date: Thu Oct 19 12:20:48 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: <20061019172040.6800.qmail@web50110.mail.yahoo.com> Right, Ventana dropped out of the running to takeover Vision. Thank goodness! So did Cytyc, Danaher is now the only bidder. You use to be able to switch barcode label with other Ventana users and your machine would think it was a new dispenser. I guess that can no longer be done with the expiration dates on the Benchmark. I will stick with my old ES. Accepting old, empty Ventana dispensers, Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From Sabina.Sylvest <@t> docsgroup.com Thu Oct 19 12:24:26 2006 From: Sabina.Sylvest <@t> docsgroup.com (Sylvest, Sabina) Date: Thu Oct 19 12:24:35 2006 Subject: [Histonet] DAKO GMS Kit Alternative Message-ID: Fellow Histologists, Does anyone know if there is another company that makes a GMS Kit suitable for placing on the DAKO Autostainer. Since DAKO has discontinued making their product and we do not have a heat source (microwave), I was hoping that another company somewhere is producing a kit that generates its own heat for a speedy stain without ancillary equipment. Thanks in advance for your help. Sabina Sylvest Lab Manager Prexus Pathology Lab 3145 Hamilton Mason Road Hamilton, OH 45011 (513) 454-1417 Sabina.Sylvest@docsgroup.com ________________________________________________ Confidentiality Notice: This electronic transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ______ Notice: auto inserted by docsgroup.com __________ From godsgalnow <@t> aol.com Thu Oct 19 13:08:46 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Oct 19 13:12:38 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: <29E7697C9175724D921A904EB1DAEEDB01095E37@mail06.eglin.af.mil> References: <29E7697C9175724D921A904EB1DAEEDB01095E37@mail06.eglin.af.mil> Message-ID: <8C8C1C0C124F136-9A8-19EA@mblk-r24.sysops.aol.com> This is exactly why we went with The Nemesis from Biocare, an open system With a closed system IHC stainer, the tech has no control. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: robert.bates@eglin.af.mil To: histonet@lists.utsouthwestern.edu Sent: Wed, 18 Oct 2006 3:23 PM Subject: [Histonet] Ventana Benchmark Problems Hello, We have a Ventana Benchmark that has been giving my histotechs fits. There are times when the staining is hit or miss. Other times the controls will not stain properly but the specimen tissue does or visa versa. We have troubleshot the machine numerous times without success. We call the aid line and do the things they recommend and it 'may' work for a while and then it reverts back to the hit or miss schedule. We have tried random tissue sections that have know positive properties (such as smooth muscle tissue stained with smooth muscle actin) and even observed internal tissue controls to see if they react properly. Has anyone else had this type of problem with this machine? Any ideas or suggestions would be most welcome. Dr. Robert R. Bates Jr Florida _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From lisab <@t> hoho.org Thu Oct 19 13:27:21 2006 From: lisab <@t> hoho.org (Lisa Brenner) Date: Thu Oct 19 13:27:56 2006 Subject: [Histonet] Re: Histonet Digest, Vol 35, Issue 31 Message-ID: Hello, We are currently installing a new IS system for pathology and need to purchase a cassette labeler to bar code cassettes. We are looking at Leica, Thermo, Sakura, and General Data. What do you use and what are your likes and dislikes? We looked at Leica 4 years ago and there were problems with it jamming up. Is that still a problem? Any feed back will be greatly appreciated. Thank you! Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hoho.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From jnocito <@t> satx.rr.com Thu Oct 19 13:31:09 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 19 13:31:29 2006 Subject: [Histonet] Ventana Benchmark Problems References: Message-ID: <003b01c6f3ac$c0c15ce0$066ace44@yourxhtr8hvc4p> nope, I heard the same thing until a Cytyk rep told me that some other company out bid them by 23 million. Some company that I've never heard of before. Couldn't remember the company if you paid me. All I know is that currently they do not have a laboratory division. I think they may make surgical or x-ray equipment. I may be wrong (I know I know, I'm admitting it publicly I may be wrong-- like that hasn't happened before). So, is it Friday yet? Joe, let's flame him, Nocito ----- Original Message ----- From: "Joanne Mauger" To: ; ; ; Sent: Thursday, October 19, 2006 10:36 AM Subject: RE: [Histonet] Ventana Benchmark Problems > To All, > > Beware-Ventana reps told me they were buying VisionBiosystems. I don't > know how true it is, but I worry about the future of the 'Bondmax' in > Ventanas hands! This is just FYI. > > Jo > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Oct 19 13:32:38 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 19 13:33:06 2006 Subject: [Histonet] Ventana Benchmark Problems. . References: <5CB39BCA5724F349BCB748675C6CA1A2099A1E84@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <005501c6f3ac$fbbc8b80$066ace44@yourxhtr8hvc4p> they're in Tucson AZ right? ----- Original Message ----- From: "Henry, Charlene" To: "Joanne Mauger" ; ; ; ; Sent: Thursday, October 19, 2006 10:53 AM Subject: RE: [Histonet] Ventana Benchmark Problems. . The bashing is brutal this morning so I might as well add my 2 cents worth. I have been using Ventana instruments for over 15 years and they have always been excellent instruments. I now have 2 Benchmark XT instruments and the slides are consistently beautiful. I have always found their tech support to be very helpful and my down-time is minimum. Are all of you using the same Ventana company that I'm using???? By the way we also have 2 Dako stainers and we do experience inconsistent staining with these instruments from time to time. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Thursday, October 19, 2006 10:36 AM To: robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu; mtarango@nvcancer.org; jnocito@satx.rr.com Subject: RE: [Histonet] Ventana Benchmark Problems. . To All, Beware-Ventana reps told me they were buying VisionBiosystems. I don't know how true it is, but I worry about the future of the 'Bondmax' in Ventanas hands! This is just FYI. Jo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Oct 19 13:33:47 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 19 13:33:59 2006 Subject: [Histonet] Ventana Purchase References: <1E3324ECFB5D5F4DAFEDD17203EEBF213FE6C3@iu-mssg-mbx105.ads.iu.edu> Message-ID: <005701c6f3ad$1e071430$066ace44@yourxhtr8hvc4p> oooooooooooooooooo you used a bad word. I'm gonna tell, get my lawyer on the phone. ----- Original Message ----- From: "Baldridge, Lee Ann" To: "fields" ; Sent: Thursday, October 19, 2006 12:16 PM Subject: RE: [Histonet] Ventana Purchase They're just going to sue them instead. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fields Sent: Thursday, October 19, 2006 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Purchase According to reliable sources this merger has fallen thru. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Oct 19 13:54:53 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Oct 19 13:52:00 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: <003b01c6f3ac$c0c15ce0$066ace44@yourxhtr8hvc4p> Message-ID: The company is Danaher. They also own a little brand called Leica Microsystems. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, October 19, 2006 2:31 PM To: Joanne Mauger; robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu; mtarango@nvcancer.org Subject: Re: [Histonet] Ventana Benchmark Problems nope, I heard the same thing until a Cytyk rep told me that some other company out bid them by 23 million. Some company that I've never heard of before. Couldn't remember the company if you paid me. All I know is that currently they do not have a laboratory division. I think they may make surgical or x-ray equipment. I may be wrong (I know I know, I'm admitting it publicly I may be wrong-- like that hasn't happened before). So, is it Friday yet? Joe, let's flame him, Nocito ----- Original Message ----- From: "Joanne Mauger" To: ; ; ; Sent: Thursday, October 19, 2006 10:36 AM Subject: RE: [Histonet] Ventana Benchmark Problems > To All, > > Beware-Ventana reps told me they were buying VisionBiosystems. I don't > know how true it is, but I worry about the future of the 'Bondmax' in > Ventanas hands! This is just FYI. > > Jo > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Thu Oct 19 13:51:53 2006 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Oct 19 13:52:18 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: Histonetters: All this talk about the Ventana Benchmark is making me nervous. We are getting ready to purchase the Ventana Discovery XT, an open system automated stainer which uses similar technology as the Benchmark. We plan to use it for research immunohistochemistry and in-situ hybridization. Does anyone have any exerience with the Discovery? Please let me know if you have had problems with it. Thanks a bunch, Linda M Histonet administrator From shiy <@t> uthscsa.edu Thu Oct 19 14:05:00 2006 From: shiy <@t> uthscsa.edu (Yun Shi) Date: Thu Oct 19 14:05:10 2006 Subject: [Histonet] Questions about muscle FIBER TYPE differentiation Message-ID: <4537CC5C.5080709@uthscsa.edu> Hi all, I'm a new member in histonet. I've been doing some IHC and IF staining on frozen muscle sections from mice. I've encountered a metachromatic dye-ATPase staining method which was originally reported by Ogilvie RW on Stain Technology (1990) Vol 65, No.5, 231-240 and subsequently used by various labs to identify muscle fiber types. In short, they claimed that using this method, all fiber types could be identified simultaneously by different intensity and homogeneity of staining. I've followed the protocol three times so far. It did not show as pretty as others. I'm wondering if anyone has tried this method and what steps or modifications need to be done to optimize it. Also how is the result compared to IHC or IF using different anti-myosin antibodies? Thanks Yun Yun Shi Physiology University of Texas Health Science Center at San Antonio From lhadley <@t> iupui.edu Thu Oct 19 14:08:36 2006 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Thu Oct 19 14:08:45 2006 Subject: [Histonet] Ventana Purchase In-Reply-To: <005701c6f3ad$1e071430$066ace44@yourxhtr8hvc4p> Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF213FE6C4@iu-mssg-mbx105.ads.iu.edu> http://boston.bizjournals.com/boston/stories/2006/09/18/daily36.html Bring 'em on!!! -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Thursday, October 19, 2006 2:34 PM To: Baldridge, Lee Ann; fields; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Purchase oooooooooooooooooo you used a bad word. I'm gonna tell, get my lawyer on the phone. ----- Original Message ----- From: "Baldridge, Lee Ann" To: "fields" ; Sent: Thursday, October 19, 2006 12:16 PM Subject: RE: [Histonet] Ventana Purchase They're just going to sue them instead. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fields Sent: Thursday, October 19, 2006 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Purchase According to reliable sources this merger has fallen thru. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Oct 19 14:19:49 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Oct 19 14:18:21 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: Message-ID: Linda, I have used the Benchmark XT for years. They are very good instruments. In my opinion the customer support is excellent. Most people either love or hate Ventana. Don't let the talk make you nervous. These discussions/debates have been going on for years. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, October 19, 2006 2:52 PM To: robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu Cc: NORA LEOS; Dinesh Rakheja; ANA GOMEZ Subject: Re: [Histonet] Ventana Benchmark Problems Histonetters: All this talk about the Ventana Benchmark is making me nervous. We are getting ready to purchase the Ventana Discovery XT, an open system automated stainer which uses similar technology as the Benchmark. We plan to use it for research immunohistochemistry and in-situ hybridization. Does anyone have any exerience with the Discovery? Please let me know if you have had problems with it. Thanks a bunch, Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Thu Oct 19 14:23:26 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Thu Oct 19 14:23:35 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: Don't be nervous. You're always going to hear the disgruntlers more often and a lot louder. I have two Benchmarks at the moment and I am getting ready to bring in an XT and an LT shortly. I've used open systems before (DAKO)and even though they were very good, I really love my Ventana's. Their customer support is second to none and they have always been very willing and able to work the little details out. I have been in my current position for almost four years and I've yet to meet my DAKO rep. We still have an autostainer that takes way too much time toset up runs in. Hope this eases your fears a little bit, Juan C. Gutierrez, HT(ASCP) Histology Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. LONG LIVE FREE SPEECH ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, October 19, 2006 1:52 PM To: robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu Cc: NORA LEOS; Dinesh Rakheja; ANA GOMEZ Subject: Re: [Histonet] Ventana Benchmark Problems Histonetters: All this talk about the Ventana Benchmark is making me nervous. We are getting ready to purchase the Ventana Discovery XT, an open system automated stainer which uses similar technology as the Benchmark. We plan to use it for research immunohistochemistry and in-situ hybridization. Does anyone have any exerience with the Discovery? Please let me know if you have had problems with it. Thanks a bunch, Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Thu Oct 19 14:24:41 2006 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Thu Oct 19 14:24:46 2006 Subject: [Histonet] Re: Histonet Digest, Vol 35, Issue 31 Message-ID: We use the Leica system for cassettes and slides, but are not using barcodes yet. However, the issue with things jamming up (and other issues) have been resolved. It has worked great since the upgrades in March of this year. Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, October 19, 2006 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 35, Issue 31 Hello, We are currently installing a new IS system for pathology and need to purchase a cassette labeler to bar code cassettes. We are looking at Leica, Thermo, Sakura, and General Data. What do you use and what are your likes and dislikes? We looked at Leica 4 years ago and there were problems with it jamming up. Is that still a problem? Any feed back will be greatly appreciated. Thank you! Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hoho.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Alice.Fallak <@t> uhsi.org Thu Oct 19 14:38:08 2006 From: Alice.Fallak <@t> uhsi.org (Fallak, Alice) Date: Thu Oct 19 14:38:16 2006 Subject: [Histonet] HT OPENING Message-ID: HI ALL, JUST WANTED TO INFORM ANYONE INTERESTED OF AN OPENING IN OUR HISTOLOGY DEPT. WE'RE AT UNITED HOSPITAL SYSTEM IN KENOSHA, WI.LET US KNOW. From amylee779 <@t> yahoo.com Thu Oct 19 14:38:29 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Oct 19 14:38:38 2006 Subject: [Histonet] histology lab design Message-ID: <20061019193829.44680.qmail@web58311.mail.re3.yahoo.com> Hello histonetter, Our facility is going to move to a new location. My boss is asking me about the histology lab design. There'll be 3 people work in this lab. For average there'll be 50-100 animal tissue ( mostly small animal ) per week need be processed for special stainning and IHC. We also need to do in-situ hybridization in the lab. We also need a suite for the scop. We will meet architect soon but I need to have a basic lab picture in my mind first. Do you know where I can search this design information? What I need to concern? Anybody has experience working with architect?... Any input is highly appreciated! Amy --------------------------------- Get your email and more, right on the new Yahoo.com From pkarlisch <@t> psu.edu Thu Oct 19 15:13:37 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Thu Oct 19 15:14:05 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: References: Message-ID: <4537A4310200008C0002AC95@GWIA02.HERSHEYMED.NET> Juan, I agree with you on this. Our folks like the Ventana, the Ventana service and the products. I was and still am a DAKO person but I have seen the two systems at work and appreciate the technical support from both companies. A1 for both. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "GUTIERREZ, JUAN" 10/19/2006 3:23 PM >>> Don't be nervous. You're always going to hear the disgruntlers more often and a lot louder. I have two Benchmarks at the moment and I am getting ready to bring in an XT and an LT shortly. I've used open systems before (DAKO)and even though they were very good, I really love my Ventana's. Their customer support is second to none and they have always been very willing and able to work the little details out. I have been in my current position for almost four years and I've yet to meet my DAKO rep. We still have an autostainer that takes way too much time toset up runs in. Hope this eases your fears a little bit, Juan C. Gutierrez, HT(ASCP) Histology Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. LONG LIVE FREE SPEECH ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, October 19, 2006 1:52 PM To: robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu Cc: NORA LEOS; Dinesh Rakheja; ANA GOMEZ Subject: Re: [Histonet] Ventana Benchmark Problems Histonetters: All this talk about the Ventana Benchmark is making me nervous. We are getting ready to purchase the Ventana Discovery XT, an open system automated stainer which uses similar technology as the Benchmark. We plan to use it for research immunohistochemistry and in-situ hybridization. Does anyone have any exerience with the Discovery? Please let me know if you have had problems with it. Thanks a bunch, Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Oct 19 15:44:41 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Oct 19 15:44:47 2006 Subject: [Histonet] Pennsylvania HistotechnologySociety Fall Meeting Update Message-ID: <6.2.5.6.2.20061019163228.01c532e8@vet.upenn.edu> Good Afternoon, This will be the last reminder for the PHS Fall Symposium in the Pittsburgh Area. The final program update with room numbers and vendors is now on the www.pahisto.org web site in both a Microsoft Word format and PDF for your review. We hope you can join us for the meeting and enjoy the program with us. We still openings in most workshops and seminars. Saturday will have classes for HT Readiness and QHIC Readiness for those who are ready to sit for the ASCP Registry. Thursday classes are devoted to areas of laboratory billing with CPT Codes, CAP Inspections for those who are under orders to be ready to bill correctly and pass an inspection. The last class in is Ergonomics and laboratory planning. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From tbraud <@t> holyredeemer.com Thu Oct 19 16:17:03 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Oct 19 16:18:35 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: <4537A4310200008C0002AC95@GWIA02.HERSHEYMED.NET> Message-ID: What a difference an individual makes! I came from a lab where our Ventana support was superb until a change in our territory rep and then nightmares ensued. As our equipment aged, we started encountering reproducibility problems with no help from our rep. We finally switched our IHC Platform, (3 Nexus modules and one great ol' ES) to 3 DAKO autostainers and received WONDERFUL service and help from DAKO, but continuing problems with the hardware mechanics and support of the remaining Benchmark XT used to run ISH only. I was a little leary as I moved to my new lab because it had just purchased a Benchmark XT, but I have found the technical and sales help here INCREDIBLE! Ventana has given us FIRST RATE support, and our immunos are consistent. Their reagent inventory system is great. Give me either system with the money to run them, and the tech help to keep them going! Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Karlisch Sent: Thursday, October 19, 2006 4:14 PM To: LINDA MARGRAF; JUAN GUTIERREZ; robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu Cc: NORA LEOS; Dinesh Rakheja; ANA GOMEZ Subject: RE: [Histonet] Ventana Benchmark Problems Juan, I agree with you on this. Our folks like the Ventana, the Ventana service and the products. I was and still am a DAKO person but I have seen the two systems at work and appreciate the technical support from both companies. A1 for both. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From jnocito <@t> satx.rr.com Thu Oct 19 17:08:43 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 19 17:09:22 2006 Subject: [Histonet] Ventana Benchmark Problems References: Message-ID: <000501c6f3cb$25089d30$066ace44@yourxhtr8hvc4p> Ms M, I don't have the Discovery, but was told when I went to Tucson that it was very similar to the XT. WE also have been having problems with their ISH kits, both with K/L and HPV. We often get variable staining with ISH also. Joe Nocito BS, PA, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "LINDA MARGRAF" To: ; Cc: "NORA LEOS" ; "Dinesh Rakheja" ; "ANA GOMEZ" Sent: Thursday, October 19, 2006 1:51 PM Subject: Re: [Histonet] Ventana Benchmark Problems Histonetters: All this talk about the Ventana Benchmark is making me nervous. We are getting ready to purchase the Ventana Discovery XT, an open system automated stainer which uses similar technology as the Benchmark. We plan to use it for research immunohistochemistry and in-situ hybridization. Does anyone have any exerience with the Discovery? Please let me know if you have had problems with it. Thanks a bunch, Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Oct 19 17:42:22 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Oct 19 17:43:52 2006 Subject: [Histonet] Ventana Purchase Message-ID: What again!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Baldridge, Lee Ann Sent: Friday, 20 October 2006 3:17 AM To: fields; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana Purchase They're just going to sue them instead. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fields Sent: Thursday, October 19, 2006 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Purchase According to reliable sources this merger has fallen thru. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From yeepengtiang <@t> hotmail.com Thu Oct 19 17:46:46 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Thu Oct 19 17:46:55 2006 Subject: [Histonet] RE: cytospin slide staining In-Reply-To: Message-ID: Hi, I have had this experience before. I am also staining the cytospin slide and I am using the HeLa cells as my control. It took me quite some time to troubleshoot the problem. In my case, the problem lies at the fixative that I've used. At first I was using 4%paraformaldehyde/PBS. This fixative is really not good at all at preserving my protein of interest. The protein lasted for only one day. I didn't know that because I did my staining on the day itself after cystospining and fixing the cells. Later on, when I did again the staining 2-3 days, I couldnt get back my positive signal. Then I decide to change the fixative, I think 10% NBF is the best but this aldehyde gives very high background in fluorescence staining. I have also tried alcohol-based fixative and found that absolute ethanol is the best. Just love it! It gives me very low (almost none) background and preserves my protein very well. In my case, I am totally in agreement with Terry on methanol. I would suggest that you try out different fixatives to see which one is suitable for your protein. With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. _________________________________________________________________ [1]Stay in touch with old friends and meet new ones with Windows Live Spaces References 1. http://g.msn.com/8HMAENUS/2737??PS=47575 From AnthonyH <@t> chw.edu.au Thu Oct 19 17:47:19 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Oct 19 17:48:51 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: This is interesting. Vision used to make quite a bit of equipment for Leica, eg their HE stainer and processor (which one, I can't remember). It seems it has gone full cycle. Just another Australian Company gobbled-up by big multinationals. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, 20 October 2006 4:55 AM To: 'Joe Nocito'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana Benchmark Problems The company is Danaher. They also own a little brand called Leica Microsystems. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, October 19, 2006 2:31 PM To: Joanne Mauger; robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu; mtarango@nvcancer.org Subject: Re: [Histonet] Ventana Benchmark Problems nope, I heard the same thing until a Cytyk rep told me that some other company out bid them by 23 million. Some company that I've never heard of before. Couldn't remember the company if you paid me. All I know is that currently they do not have a laboratory division. I think they may make surgical or x-ray equipment. I may be wrong (I know I know, I'm admitting it publicly I may be wrong-- like that hasn't happened before). So, is it Friday yet? Joe, let's flame him, Nocito ----- Original Message ----- From: "Joanne Mauger" To: ; ; ; Sent: Thursday, October 19, 2006 10:36 AM Subject: RE: [Histonet] Ventana Benchmark Problems > To All, > > Beware-Ventana reps told me they were buying VisionBiosystems. I don't > know how true it is, but I worry about the future of the 'Bondmax' in > Ventanas hands! This is just FYI. > > Jo > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From itzel14 <@t> yahoo.com Thu Oct 19 18:48:04 2006 From: itzel14 <@t> yahoo.com (itzel calleja) Date: Thu Oct 19 18:48:08 2006 Subject: [Histonet] LCM and RNA extraction Message-ID: <20061019234804.43443.qmail@web30303.mail.mud.yahoo.com> HISTONETTERS: I?m looking for a RNA extraction protocol. I have FFPE LCM samples. Please let me know. Thanks Itzel Itzel Evelyn Calleja Macias, PhD Molecular Biology & Biochemistry University of California Irvine 150 Sprague Hall Irvine, CA 92697 Tel: (949) 824-2435 Fax: (949) 824-8551 itzel14@yahoo.com --------------------------------- Do You Yahoo!? La mejor conexi?n a Internet y 2GB extra a tu correo por $100 al mes. http://net.yahoo.com.mx From RSRICHMOND <@t> aol.com Thu Oct 19 20:36:22 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Oct 19 20:36:30 2006 Subject: [Histonet] setting up immunohistochemistry Message-ID: <589.3b305f1.32698216@aol.com> I've retired from full time employment to pursue my previous peripatetic existence as the Samurai Pathologist. I'm being asked to look into seeing up immunohistochemistry for a rather isolated solo pathology practice. The service has three histotechnologists, one of whom is capable of working with IHC (the other two are very near retirement). 1. How many billable slides a year does the service have to expect to produce, to justify the cost of running an IHC service? 2. There are I think three competing instruments available in the USA. Which of these would you suggest for a service such as the one I describe? How do you go about deciding among them? Bob Richmond Samurai Pathologist Knoxville TN (licensed TN NC GA MO KY) From mbmphoto <@t> gmail.com Thu Oct 19 20:58:28 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Oct 19 20:57:24 2006 Subject: [Histonet] fresh frozen brain controls Message-ID: <339A9AC2-A0CF-4C2F-9A68-5C33427CBCE8@gmail.com> Please could someone tell me where I can obtain or buy some fresh frozen brain controls slides. I need fresh frozen slides from the following brain area: > substantia nigra area - brain (human) normal. > substantia nigra area - brain (human) Parkinson Disease. > striatum area - brain (human) normal. > striatum area - brain (human) Parkinson Disease. I would greatly appreciated any help & assistance anyone can provide. I look forward to hearing from someone. Maria Bartola Mejia UCSF Department of Neurosurgery San Francisco, CA 94103 Lab Phone: (415)-514-2954 From anh2006 <@t> med.cornell.edu Fri Oct 20 00:08:57 2006 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Oct 20 00:09:08 2006 Subject: [Histonet] fresh frozen brain controls Message-ID: <9e96625f4f1.453821a9@med.cornell.edu> Try the Cooperative Human Tissue Network (CHTN). They take all sorts of specialized requests though for normal brain I expect it will be taken post-mortem. In any case, they can work it out with you to your specifications. http://www-chtn.ims.nci.nih.gov/index.html ----- Original Message ----- From: Maria Mejia Date: Thursday, October 19, 2006 9:58 pm Subject: [Histonet] fresh frozen brain controls > Please could someone tell me where I can obtain or buy some fresh > frozen brain > controls slides. I need fresh frozen slides from the following > brain > area: > > > substantia nigra area - brain (human) normal. > > > substantia nigra area - brain (human) Parkinson Disease. > > > striatum area - brain (human) normal. > > > striatum area - brain (human) Parkinson Disease. > > I would greatly appreciated any help & assistance anyone can > provide. > I look forward > to hearing from someone. > > Maria Bartola Mejia > UCSF > Department of Neurosurgery > San Francisco, CA 94103 > Lab Phone: (415)-514-2954 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jqb7 <@t> cdc.gov Fri Oct 20 08:04:51 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Oct 20 08:12:28 2006 Subject: [Histonet] decalcification question Message-ID: Hi all, We are need of information regarding the best decal procedure for whole ferret heads. We have been using a commercial decal/fixative combination but it is a very slow process. Any suggestions will be greatly appreciated. Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From Barry.R.Rittman <@t> uth.tmc.edu Fri Oct 20 08:26:28 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Oct 20 08:26:34 2006 Subject: [Histonet] decalcification question Message-ID: Jeanine In my experience, solutions that combine a fixative and decalcification agent are very slow. In general the problem with a prolonged decalcification e.g. because the specimen is large, is the risk of maceration due to some reversal of the bonds formed during fixation. If you are using EDTA you can periodically remove the specimen from the solution and replace in the fixative overnight, i.e. a "refixation". This is much more rapid than using the combination solution and the results, in my hands have been as good. What end result do you wish to achieve? -immunohistochemistry, paraffin or frozen sections, gross examination. Have the heads been treated for example by perfusion to show blood vessels? Thanks Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, October 20, 2006 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification question Sensitivity: Confidential Hi all, We are need of information regarding the best decal procedure for whole ferret heads. We have been using a commercial decal/fixative combination but it is a very slow process. Any suggestions will be greatly appreciated. Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lange <@t> kennedykrieger.org Fri Oct 20 09:23:29 2006 From: lange <@t> kennedykrieger.org (Mollie Lange) Date: Fri Oct 20 09:23:57 2006 Subject: [Histonet] gelatin-albumin embedding Message-ID: <4538A398.BB3C.0024.0@kennedykrieger.org> Dear Histonetters, Does anyone have a protocol for gelatin-albumin embedding of brain tissue? Or can you direct me to a text or article that might have information? We are doing Golgi stains on neonatal rat brain in vibratome sections and need the support for the tissue that this embedding provides. We appreciate all responses. Mollie Lange International Center for Spinal Cord Injury Kennedy Krieger Institute Baltimore, Maryland Disclaimer: The materials in this e-mail are private and may contain Protected Health Information. Please note that e-mail is not necessarily confidential or secure. Your use of e-mail constitutes your acknowledgment of these confidentiality and security limitations. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via telephone or return e-mail. From Janet.Bonner <@t> FLHOSP.ORG Fri Oct 20 09:56:21 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Oct 20 09:57:00 2006 Subject: [Histonet] Acetylcholinesterase Procedure Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A64E@fhosxchmb006.ADVENTISTCORP.NET> Dear All, Does anyone know where an Acetylcholinesterase Procedure Kit can be purchased? Thank you! Janet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Janet.Bonner <@t> FLHOSP.ORG Fri Oct 20 10:00:28 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Oct 20 10:01:47 2006 Subject: [Histonet] Acetylcholinesterase Procedure References: <5F31F38C96781A4FBE3196EBC22D478004A64E@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A64F@fhosxchmb006.ADVENTISTCORP.NET> Also...is the immunoperoxidase Procedure superior to the Histology Procedure? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bonner, Janet Sent: Fri 10/20/2006 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acetylcholinesterase Procedure Dear All, Does anyone know where an Acetylcholinesterase Procedure Kit can be purchased? Thank you! Janet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From djohnson14 <@t> hotmail.com Fri Oct 20 10:13:47 2006 From: djohnson14 <@t> hotmail.com (Dave Johnson) Date: Fri Oct 20 10:14:00 2006 Subject: [Histonet] Redisharp marking pens Message-ID: To stir an old pot, what were previous opinions of the Redisharp pens for marking both cassettes AND slides. Was this one of the better pens that USE to be available. I understand that it no longer is,j ust wondering past experiences people had with that product Dave _________________________________________________________________ Add a Yahoo! contact to Windows Live Messenger for a chance to win a free trip! http://www.imagine-windowslive.com/minisites/yahoo/default.aspx?locale=en-us&hmtagline From tbraud <@t> holyredeemer.com Fri Oct 20 11:32:43 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Oct 20 11:35:08 2006 Subject: [Histonet] Redisharp marking pens In-Reply-To: Message-ID: I don't know about those, but we were given a sample of Surgipath's marking pens and everyone here luvs 'em! They're bright, sharp, don't fade at all, and are slow to dry out when left uncapped (not, of course, that we ever do) Just thought I'd tout a good product - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dave Johnson Sent: Friday, October 20, 2006 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Redisharp marking pens To stir an old pot, what were previous opinions of the Redisharp pens for marking both cassettes AND slides. Was this one of the better pens that USE to be available. I understand that it no longer is,j ust wondering past experiences people had with that product Dave http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From ttroyer <@t> petersonlab.com Fri Oct 20 11:37:07 2006 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Fri Oct 20 11:37:15 2006 Subject: [Histonet] Lymph Node Frozen Sections Message-ID: <000801c6f465$fbfc7ba0$6601010a@Peterson.local> I am a novice when it comes to lymph node frozen section, and I was wondering if anyone had any helpful hints on cutting the fatty tissue. I was wondering if any has tried fixing the tissue in alcohol or some other fixative and then using a charged slide? Thanks, Travis From GoodwinD <@t> pahosp.com Tue Oct 17 11:52:24 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Oct 20 11:38:40 2006 Subject: [Histonet] Phospho-S6 Ribosomal Protein Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8235@uphsmbx2.UPHS.PENNHEALTH.PRV> For those of us 'Netters using this Ab on human tissue, which one and at what dilution? Thanks! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From George.Adams <@t> UTSouthwestern.edu Wed Oct 18 13:49:43 2006 From: George.Adams <@t> UTSouthwestern.edu (George Adams) Date: Fri Oct 20 11:51:37 2006 Subject: [Histonet] Alcian Blue vs. Safranin-O for Human Umbilical Vein Tissue Message-ID: <453630F8020000180000816A@swnw126.swmed.edu> HISTONETTERS: I am trying to find out a detailed comparison of Safranin-O versus Alcian blue stains for glycosaminoglycans (GAGs) and proteoglycans. Specifically, does Safranin-O stain sulfated (chondroitin sulfate) or only non-sulfated GAGs (hyaluronic acid)? Is there an advantage or disadvantage to using Alcian blue for the same GAGs? The tissue that one of the students in our lab will be using is from human umbilical cord. I received a very nice and quick response from Mr. Charles Churukian via Mr. Matthew Frank at the the University of Rochester Medical Center which is in bold below. Mr. Adams, I was just informed by Charles Churukian that Alcian Blue is the correct stain to use for an umbilical cord section. Safranin O has been used for bone tissue but for this application Alcain Blue is your stain. I have no info on comparison studies between the two. Matthew Frank 585-275-2751 Looking forward to everyone's responses. George Adams, Ph.D. Dept. of Otolaryngology From jcline <@t> wchsys.org Fri Oct 20 12:21:07 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Oct 20 12:21:27 2006 Subject: [Histonet] Ventana Message-ID: <001c01c6f46c$21f09ca0$1d2a14ac@wchsys.org> Hi All, I have used the Ventana Special Stainer and the NexEs IHC models for 6 years. I have had excellent problem solving, repair turnaround, and the nicest sales reps. I am now validating a Benchmark XT and I find it works as well as the other systems. For my lab, the walk away feature from these instruments is what I needed. The walk away feature helps with staffing issues (our lab is trying not to replace our A.P. assistant so the techs are now doing the specimen accessioning) Which everyone knows takes a lot of time away from technical issues. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Oct 20 12:48:07 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Oct 20 12:47:55 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 33 Message-ID: Dear Net: Has any one used Surgipath's Sta-On(r) Tissue Section Adhesive? For frozen sections? Does it help keep sections stuck to the slide? Thanks, Sarah Pixley Ohio From juan.gutierrez <@t> christushealth.org Fri Oct 20 13:08:50 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Oct 20 13:08:57 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 33 Message-ID: Not for frozen sections, but for paraffin sections, yes. If you are using charged slides, stay away from Sta-on. We had all kinds of problems with sections falling off when we used it with coated slides. Get yourself some nice coated (charged) slides and you shouldn't have to worry about using additives. Hope this helps. Juan C. Gutierrez, HT(ASCP) Histology Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. LONG LIVE FREE SPEECH ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pixley, Sarah (pixleysk) Sent: Friday, October 20, 2006 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 33 Dear Net: Has any one used Surgipath's Sta-On(r) Tissue Section Adhesive? For frozen sections? Does it help keep sections stuck to the slide? Thanks, Sarah Pixley Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Oct 20 13:14:57 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Oct 20 13:15:05 2006 Subject: [Histonet] TGIF Message-ID: <20061020181457.91035.qmail@web37710.mail.mud.yahoo.com> Yahoo.....it's Friday....Margarita-ville tonight.... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From mtarango <@t> nvcancer.org Fri Oct 20 13:24:15 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Oct 20 13:24:35 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: <5AEC610C1CE02945BD63A395BA763EDEB1CFB4@NVCIEXCH02.NVCI.org> Well you won't be able to use Vysis probes with the discovery. Mark T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, October 19, 2006 11:52 AM To: robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu Cc: NORA LEOS; Dinesh Rakheja; ANA GOMEZ Subject: Re: [Histonet] Ventana Benchmark Problems Histonetters: All this talk about the Ventana Benchmark is making me nervous. We are getting ready to purchase the Ventana Discovery XT, an open system automated stainer which uses similar technology as the Benchmark. We plan to use it for research immunohistochemistry and in-situ hybridization. Does anyone have any exerience with the Discovery? Please let me know if you have had problems with it. Thanks a bunch, Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From ASmid <@t> mednet.ucla.edu Fri Oct 20 13:32:45 2006 From: ASmid <@t> mednet.ucla.edu (Smid, Alojz) Date: Fri Oct 20 13:33:00 2006 Subject: [Histonet] RE: fresh frozen brain controls Message-ID: <85042C7A1A0DD74EACF3145F32F37BA410EB45E1@medmail6.mednet.ucla.edu> /Please could someone tell me where I can obtain or buy some fresh /frozen brain /controls slides. I need fresh frozen slides from the following brain This might be worth a look: http://www.loni.ucla.edu/uclabrainbank/ L. ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From jqb7 <@t> cdc.gov Fri Oct 20 13:23:03 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Oct 20 13:35:00 2006 Subject: [Histonet] TGIF Message-ID: I'm a martini gal myself, but cheers! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, October 20, 2006 2:15 PM To: Histonet Subject: [Histonet] TGIF Yahoo.....it's Friday....Margarita-ville tonight.... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Wrona <@t> kp.org Fri Oct 20 13:06:14 2006 From: Erin.Wrona <@t> kp.org (Erin.Wrona@kp.org) Date: Fri Oct 20 13:41:11 2006 Subject: [Histonet] Redisharp marking pens Message-ID: "I don't know about those, but we were given a sample of Surgipath's marking pens and everyone here luvs 'em! They're bright, sharp, don't fade at all, and are slow to dry out when left uncapped (not, of course, that we ever do) Just thought I'd tout a good product - Terri Terri L. Braud, HT(ASCP)" I agree - we just switched to these from the Secureline Superfrost pens. The Surgipath pens are much brighter and don't dry out as fast. Everyone here likes them... Erin Wrona, HT (ASCP) NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From lblazek <@t> digestivespecialists.com Fri Oct 20 13:51:52 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 20 13:48:16 2006 Subject: [Histonet] TGIF Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684B58@bruexchange.digestivespecialists.com> I think I need a bottle of wine and a long hot bubble bath. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, October 20, 2006 2:23 PM To: Kim Tournear; Histonet Subject: RE: [Histonet] TGIF I'm a martini gal myself, but cheers! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, October 20, 2006 2:15 PM To: Histonet Subject: [Histonet] TGIF Yahoo.....it's Friday....Margarita-ville tonight.... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Oct 20 14:37:09 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 20 14:37:27 2006 Subject: [Histonet] Lymph Node Frozen Sections Message-ID: <4EBFF65383B74D49995298C4976D1D5E273B99@LSRIEXCH1.lsmaster.lifespan.org> I cut lymph nodes at -12 to -14 degrees. Much colder than that and they will crack. This works well for both fresh frozen and formalin-fixed tissue. You can't use an alcoholic fixative prior to frozen sectioning unless you make sure you wash out ALL the fixative before freezing. Even a small concentration of alcohol in the tissue will act as antifreeze, and the tissue will be too soft to section. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Travis Troyer > Sent: Friday, October 20, 2006 9:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lymph Node Frozen Sections > > I am a novice when it comes to lymph node frozen section, and I was > wondering if anyone had any helpful hints on cutting the fatty tissue. > I was wondering if any has tried fixing the tissue in alcohol or some > other fixative and then using a charged slide? > > Thanks, > Travis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From bayoubelle311 <@t> gmail.com Fri Oct 20 14:40:17 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Fri Oct 20 14:40:29 2006 Subject: [Histonet] EDTA Decal endpoint Message-ID: <398f02c20610201240yc4999ch2b665c86cb649451@mail.gmail.com> Hi all, I am about to start decalcifying rat femur and sternum for myelofibrosis evaulation through the use of EDTA. (unless anyone has another maybe faster methhod that will not destroy reticulin or interfere is Gomori Silver stain). I wanted to know if there was a definitive endpoint, rather than "when the bone is floppy" etc. I spoke with a colleague who said there may be a commercially available testing method, by color change. Is this true, and if it it does exist, what is it called? Thanks Missy From HornHV <@t> archildrens.org Fri Oct 20 14:42:21 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Oct 20 14:42:56 2006 Subject: [Histonet] setting up immunohistochemistry In-Reply-To: <589.3b305f1.32698216@aol.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6E35@EMAIL.archildrens.org> We are a small lab and we do our own immunos. We do send a few out that we do not perform often. We have around a 40 antibody menu. We average 80 a month. We use the Dako Autostainer. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Thursday, October 19, 2006 8:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] setting up immunohistochemistry I've retired from full time employment to pursue my previous peripatetic existence as the Samurai Pathologist. I'm being asked to look into seeing up immunohistochemistry for a rather isolated solo pathology practice. The service has three histotechnologists, one of whom is capable of working with IHC (the other two are very near retirement). 1. How many billable slides a year does the service have to expect to produce, to justify the cost of running an IHC service? 2. There are I think three competing instruments available in the USA. Which of these would you suggest for a service such as the one I describe? How do you go about deciding among them? Bob Richmond Samurai Pathologist Knoxville TN (licensed TN NC GA MO KY) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From jnocito <@t> satx.rr.com Fri Oct 20 14:46:37 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Oct 20 14:46:50 2006 Subject: [Histonet] Good Bye Message-ID: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito From Barry.R.Rittman <@t> uth.tmc.edu Fri Oct 20 15:12:10 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Oct 20 15:12:17 2006 Subject: [Histonet] Good Bye Message-ID: Joe Hang on in there. You are our hero. Proud that you have the courage to stand up for your convictions. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 20, 2006 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Zajic <@t> HCAhealthcare.com Fri Oct 20 15:13:33 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Fri Oct 20 15:13:39 2006 Subject: [Histonet] Good Bye In-Reply-To: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC77D@ORLEV03.hca.corpad.net> That's extremely unfortunate Joe, I won't ever be using said vendor. Good luck and keep your head up. Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, October 20, 2006 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Fri Oct 20 15:14:29 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Fri Oct 20 15:14:39 2006 Subject: [Histonet] Thanks for helping histology lab design! Message-ID: <20061020201429.21571.qmail@web58307.mail.re3.yahoo.com> Hello, Many thanks to everyone who gave me advice on histology lab design! They are very helpful! I printed out all of them in case anyone need them later so I can fax to you. They are excellent suggestions! Thanks again! Amy --------------------------------- Get your email and more, right on the new Yahoo.com From godsgalnow <@t> aol.com Fri Oct 20 15:19:24 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Oct 20 15:19:43 2006 Subject: [Histonet] job descriptions Message-ID: <8C8C29C2B44B940-950-5B96@mblk-r32.sysops.aol.com> Anyone out there willing to share there job description for hist lab aide? Thanks in advance, Roxanne Soto Lab Supervisor ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From katri <@t> cogeco.ca Fri Oct 20 15:26:38 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Oct 20 15:26:49 2006 Subject: [Histonet] Good Bye References: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: <006c01c6f486$0c2b30f0$6a9a9618@Katri> Joe! What do you mean "Good bye"? Did your employer gag you from histonet? Incredible! What do you think folks? Where's the freedom of speech? Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Joe Nocito" To: Sent: Friday, October 20, 2006 3:46 PM Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynde77627 <@t> aol.com Fri Oct 20 15:30:52 2006 From: Lynde77627 <@t> aol.com (Lynde77627@aol.com) Date: Fri Oct 20 15:31:21 2006 Subject: [Histonet] Good Bye Message-ID: Don't go Joe! Your input and humor is vital to a lot of us here. This is a site for important exchanges of information and what you have contributed and can continue to contribute is necessary. If some vendors have a problem with the open exchanges of information then they should quit monitoring the list. From DavidH <@t> marketlabinc.com Fri Oct 20 15:38:56 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Fri Oct 20 15:39:04 2006 Subject: [Histonet] Good Bye Message-ID: <19E3602A16438E48B51A4250CA04B5F68CD187@exchange.marketlab.com> Some things just aren't right in this world. A vendor going after Joe and getting him in trouble for speaking the truth is way, way out of bounds. What they should have done instead is send their rep in to optimize his system and make it work the best they can for him. Now when the rep shows up they're going to find out how he got the nickname - "The Toe". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 20, 2006 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. From victor <@t> pathology.washington.edu Fri Oct 20 15:45:39 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Oct 20 15:45:49 2006 Subject: [Histonet] Good Bye In-Reply-To: <19E3602A16438E48B51A4250CA04B5F68CD187@exchange.marketlab.com> References: <19E3602A16438E48B51A4250CA04B5F68CD187@exchange.marketlab.com> Message-ID: <45393573.4060202@pathology.washington.edu> Anybody know who the vendor is? Victor David Haagsma wrote: > Some things just aren't right in this world. A vendor going after Joe > and getting him in trouble for speaking the truth is way, way out of > bounds. What they should have done instead is send their rep in to > optimize his system and make it work the best they can for him. Now when > the rep shows up they're going to find out how he got the nickname - > "The Toe". > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 20, 2006 3:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Good Bye > > Just as I expected, some one called my place of employment. Not only > did I get flamed, I was burned to a crisp. I hope you are happy. You > think you may have won, but all you did was hamper a so called open > forum so people could exchange ideas and opinions. Now, many people will > be afraid to give their honest opinion for fear of retribution, Sleep > tight tonight. You almost cost me my job. Losing my job over a vendor is > just not worth it. I don't need any help, I can do it just fine by > myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that people > can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good > luck. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From dahui_you <@t> yahoo.com Fri Oct 20 15:41:54 2006 From: dahui_you <@t> yahoo.com (Dahui You) Date: Fri Oct 20 15:48:49 2006 Subject: [Histonet] Just say thanks! Message-ID: <20061020204154.84985.qmail@web35714.mail.mud.yahoo.com> Hi, histonetter: Just say thank you all for your kind reponses to my former emails. Thank you guys very much and have a nice weekend! Dahui You LSU From doug <@t> ppspath.com Fri Oct 20 15:52:31 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Oct 20 15:49:36 2006 Subject: [Histonet] Good Bye In-Reply-To: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: Joe, I do not share your opinion of said vendor but I know how you are feeling. I voiced my opinion on a certain tissue processor and one of the company's people went behind my back and called my boss. We found it quit amusing but it shows you how low they can go to try to save their reputation by trying to ruin yours. If these companies can't take criticism or step up to the plate on this forum and defend themselves then they are in the wrong field. There is always going to be competition and preference in this field like Sakura vs Leica, Ventana vs Dako, Ford vs Chevy, and Pepsi vs Coke. I am not sure if these or the other company's front offices support these types of actions but I hope they get their vendors under control before they damage the company's reputation even further. This post does not represent the opinion of my employer, my wife, my dog, my cat, or me. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 20, 2006 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Histologia <@t> aol.com Fri Oct 20 15:50:25 2006 From: Histologia <@t> aol.com (Histologia@aol.com) Date: Fri Oct 20 15:50:30 2006 Subject: [Histonet] Good Bye Message-ID: <4bf.2b90cc3.326a9091@aol.com> Joe has been a great leader,contributor, and gentleman on the histonet. His professionalism shows in his dedication to the patients he serves. I can only hope that when I have to have my own histology specimen taken care of that it lands in the hands of Joe. For all of those who are intimidated by his knowledge and leadership skills I suggest you spend some time with him and develop some of your own. A fellow histonetter and follower of Joe Terry From JMahoney <@t> alegent.org Fri Oct 20 16:02:17 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Fri Oct 20 16:07:21 2006 Subject: [Histonet] Good Bye Message-ID: Good point Douglas, I doubt that Joe is gone forever. We need his humor on this net so I certainly hope he is not gone for long. I think that any vendor is going to get as many praises as they do criticisms. No one company can meet every need and expectation. I have a hard time believing that a vendor, who needs our support as much as we need them, would risk their reputation by calling an employer. Those kind of actions spread like wild fire and have a way of coming around and biting you on the behind. Come back soon Joe. Jan Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Douglas D Deltour" 10/20/2006 3:52 PM >>> Joe, I do not share your opinion of said vendor but I know how you are feeling. I voiced my opinion on a certain tissue processor and one of the company's people went behind my back and called my boss. We found it quit amusing but it shows you how low they can go to try to save their reputation by trying to ruin yours. If these companies can't take criticism or step up to the plate on this forum and defend themselves then they are in the wrong field. There is always going to be competition and preference in this field like Sakura vs Leica, Ventana vs Dako, Ford vs Chevy, and Pepsi vs Coke. I am not sure if these or the other company's front offices support these types of actions but I hope they get their vendors under control before they damage the company's reputation even further. This post does not represent the opinion of my employer, my wife, my dog, my cat, or me. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 20, 2006 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lrichey <@t> u.washington.edu Fri Oct 20 16:13:36 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Fri Oct 20 16:13:48 2006 Subject: [Histonet] Good Bye In-Reply-To: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> References: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: <45393C00.1030900@u.washington.edu> Sorry to hear about this! Since when do the vendors censor the histonet, a venue for technicians and technologists to communicate amongst themselves. This is the USA, we do have freedom of speech. Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > >Joe Nocito >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From bwhitaker <@t> brownpathology.com Fri Oct 20 16:30:06 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Oct 20 16:26:34 2006 Subject: [Histonet] Good Bye In-Reply-To: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: <000801c6f48e$e9b25630$3601a8c0@brownpathology.net> Joe, What do you expect from said company? There ARE some good people that work for them.... I am friends with some of them. I admire their ability to stick it out, but the corporate philosophy there sucks! No vendor that uses tactics like theirs will ever be welcome in my laboratory. The same goes for any company that does not consider what is best for the lab, and ultimately the patient when doing business. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 20, 2006 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Fri Oct 20 16:33:40 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Oct 20 16:33:51 2006 Subject: [Histonet] Good Bye In-Reply-To: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> References: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: <453940B4.6030509@ebsciences.com> And now a vendor weighs in (NOT a vendor involved in this particular episode, I hasten to add): I'm frankly surprised; as a manufacturer of histology equipment under our own name, and for others, I look at an end user's expression of dissatisfaction as an opportunity to make right. It is valuable, albeit unsolicited, feedback. I've passed on more than one comment about less-than-stellar service to companies whom we have alliances with, NOT to quash dissent, but to show there's room for improvement. Hopefully it's "au revoir" rather than "good-bye" to Joe. -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" You must be the change you want to see in the world. - Mahatma Gandhi Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > > Joe Nocito > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mmcgraw <@t> seattlecca.org Fri Oct 20 17:02:10 2006 From: mmcgraw <@t> seattlecca.org (McGraw, Medea J) Date: Fri Oct 20 17:02:22 2006 Subject: [Histonet] RE: Using the best Retic Silver Stain for Bone marrow biopsies Message-ID: <095BC97447B56C489B3498DE0B30CDD50F3180@storm.seattlecca.org> I am searching for an optimal method for providing the best Retic silver stain, aside from using automation and kits, on bone marrow biopsies. Currently, we have been using the Wilder's technique and ran into inconsistencies in the past 5 months or so. Is there a better method than your lab is currently using that you would care to share with me to try? I have also tried the Gridley's and the results are good fibers but very muddy. We are currently using a Nexus to help us "get by" but the kits are $$$ and it's just a temporary fix. I am interested in all methods (microwave or not). I would appreciate any suggestions. This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From asamra <@t> mrl.ubc.ca Fri Oct 20 17:14:06 2006 From: asamra <@t> mrl.ubc.ca (Amrit Samra) Date: Fri Oct 20 17:15:43 2006 Subject: [Histonet] unsbscribe Message-ID: <4538E7BE0200008D0000BA5A@mail.mrl.ubc.ca> Unsubscribe me please From mtarango <@t> nvcancer.org Fri Oct 20 18:59:22 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Oct 20 18:59:37 2006 Subject: [Histonet] Good Bye Message-ID: <5AEC610C1CE02945BD63A395BA763EDEC99F28@NVCIEXCH02.NVCI.org> VENTANA MEDICAL SYSTEMS. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Friday, October 20, 2006 1:46 PM To: David Haagsma Cc: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Good Bye Anybody know who the vendor is? Victor David Haagsma wrote: > Some things just aren't right in this world. A vendor going after Joe > and getting him in trouble for speaking the truth is way, way out of > bounds. What they should have done instead is send their rep in to > optimize his system and make it work the best they can for him. Now when > the rep shows up they're going to find out how he got the nickname - > "The Toe". > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 20, 2006 3:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Good Bye > > Just as I expected, some one called my place of employment. Not only > did I get flamed, I was burned to a crisp. I hope you are happy. You > think you may have won, but all you did was hamper a so called open > forum so people could exchange ideas and opinions. Now, many people will > be afraid to give their honest opinion for fear of retribution, Sleep > tight tonight. You almost cost me my job. Losing my job over a vendor is > just not worth it. I don't need any help, I can do it just fine by > myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that people > can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good > luck. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From alaskagirl1950 <@t> yahoo.com Fri Oct 20 23:21:52 2006 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Oct 20 23:21:57 2006 Subject: [Histonet] Good Bye In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDEC99F28@NVCIEXCH02.NVCI.org> Message-ID: <20061021042152.52330.qmail@web52506.mail.yahoo.com> Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From billingconsultants <@t> yahoo.com Sat Oct 21 06:25:37 2006 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Sat Oct 21 07:25:44 2006 Subject: [Histonet] Re: setting up immunohistochemistry Message-ID: <20061021112537.64580.qmail@web54214.mail.yahoo.com> As a consultant with a pathology billing/consulting firm, I have set up immuno programs at various scales of production. For a small lab, with a production of 2-5 cases/day, you can very cost effectively perform immunos manually. As the volume of immuno cases increases, then the cost of automation is usually easily absorbed. Of course, the success of performing immunos manually relies a lot on the competency of the technician performing them. If the histology staff is less experienced, automating the process is the best thing to do. Kindest regards, Louri Roberts Consultant Billing Consultants, LLC www.billingconsultants.net --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Sat Oct 21 07:56:07 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 21 07:56:15 2006 Subject: [Histonet] RE: Using the best Retic Silver Stain for Bone marrow biopsies In-Reply-To: <095BC97447B56C489B3498DE0B30CDD50F3180@storm.seattlecca.org> Message-ID: <20061021125607.41159.qmail@web61222.mail.yahoo.com> Medea J.: In my experience, after trying many different procedures, I found that the best and more consistent is Gomori's ammoniacal silver method. Ren? J. "McGraw, Medea J" wrote: I am searching for an optimal method for providing the best Retic silver stain, aside from using automation and kits, on bone marrow biopsies. Currently, we have been using the Wilder's technique and ran into inconsistencies in the past 5 months or so. Is there a better method than your lab is currently using that you would care to share with me to try? I have also tried the Gridley's and the results are good fibers but very muddy. We are currently using a Nexus to help us "get by" but the kits are $$$ and it's just a temporary fix. I am interested in all methods (microwave or not). I would appreciate any suggestions. This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From cmiller <@t> physlab.com Sat Oct 21 08:33:22 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Sat Oct 21 08:33:32 2006 Subject: [Histonet] Good Bye In-Reply-To: <20061021042152.52330.qmail@web52506.mail.yahoo.com> Message-ID: <000001c6f515$7c4801d0$db01a8c0@plab.local> I am speechless. I can't believe someone can almost lose their job by giving their opinion. That is why we are all here to share and learn from one another. I can see if you were trashing YOUR boss, but this?? Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Friday, October 20, 2006 11:22 PM To: Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marjoh3 <@t> telus.net Sat Oct 21 08:48:27 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Oct 21 08:48:28 2006 Subject: [Histonet] Special Stains For Clostridium Message-ID: <001201c6f517$95e7b0c0$6401a8c0@VALUED20606295> Hi Histonetters, Hope someone can provide suggestions for special stains that will demonstrate Clostridium hemolyticum. These organisms can be seen on H&E, yet not satisfactory by Brown & Brenn Gram stain. I have considered the Giemsa and Warthin-Starrey stains. Bovine liver sections will be taken for this project. Any comments would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada. From pruegg <@t> ihctech.net Sat Oct 21 11:02:15 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Oct 21 11:02:27 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: Message-ID: <200610211602.k9LG2EQI001091@pro12.abac.com> I heard from a reliable source that Danaher has made a larger bid for Vision, not sure Ventana is in the running anymore to buy Vision. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, October 19, 2006 4:47 PM To: Douglas D Deltour; Joe Nocito; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana Benchmark Problems This is interesting. Vision used to make quite a bit of equipment for Leica, eg their HE stainer and processor (which one, I can't remember). It seems it has gone full cycle. Just another Australian Company gobbled-up by big multinationals. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, 20 October 2006 4:55 AM To: 'Joe Nocito'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana Benchmark Problems The company is Danaher. They also own a little brand called Leica Microsystems. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, October 19, 2006 2:31 PM To: Joanne Mauger; robert.bates@eglin.af.mil; histonet@lists.utsouthwestern.edu; mtarango@nvcancer.org Subject: Re: [Histonet] Ventana Benchmark Problems nope, I heard the same thing until a Cytyk rep told me that some other company out bid them by 23 million. Some company that I've never heard of before. Couldn't remember the company if you paid me. All I know is that currently they do not have a laboratory division. I think they may make surgical or x-ray equipment. I may be wrong (I know I know, I'm admitting it publicly I may be wrong-- like that hasn't happened before). So, is it Friday yet? Joe, let's flame him, Nocito ----- Original Message ----- From: "Joanne Mauger" To: ; ; ; Sent: Thursday, October 19, 2006 10:36 AM Subject: RE: [Histonet] Ventana Benchmark Problems > To All, > > Beware-Ventana reps told me they were buying VisionBiosystems. I don't > know how true it is, but I worry about the future of the 'Bondmax' in > Ventanas hands! This is just FYI. > > Jo > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Sat Oct 21 11:11:37 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Sat Oct 21 11:12:05 2006 Subject: [Histonet] Good Bye In-Reply-To: <20061020205048.2E4F015EC03@smtpgrey-2.iphouse.net> Message-ID: <20061021161156.EAF592AC58A@smtpout-2.iphouse.net> Another vendor chimes in... Other than Joe and the vendor in question, none of us knows all the details, but if this tactic was used... it is very poor form on the part of the vendor. This is an open forum and if the comments or opinions expressed amount to erroneous facts or half-truths, then vendor is free to post a rebuttal, or forever keep their silence... especially to one's employer. I will now practice what I preach. As for me, and just for the record, I want to make it perfectly clear... Even though I am the current owner of an Oldsmobile... I categorically deny that I have ever had anything bad to say against Chevy. (see post below.) ~ Ford ;-) Ford M. Royer, MT(ASCP) Sales Manager, Histology Products Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427 Phone: 888-790-9686 Fax: 763-546-4830 Cell: 612-839-1046 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, October 20, 2006 3:53 PM To: 'Joe Nocito'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Good Bye Joe, I do not share your opinion of said vendor but I know how you are feeling. I voiced my opinion on a certain tissue processor and one of the company's people went behind my back and called my boss. We found it quit amusing but it shows you how low they can go to try to save their reputation by trying to ruin yours. If these companies can't take criticism or step up to the plate on this forum and defend themselves then they are in the wrong field. There is always going to be competition and preference in this field like Sakura vs Leica, Ventana vs Dako, "Ford vs Chevy", and Pepsi vs Coke. I am not sure if these or the other company's front offices support these types of actions but I hope they get their vendors under control before they damage the company's reputation even further. This post does not represent the opinion of my employer, my wife, my dog, my cat, or me. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 From snapper1532 <@t> hotmail.com Sat Oct 21 12:15:55 2006 From: snapper1532 <@t> hotmail.com (Snapper Allen) Date: Sat Oct 21 12:16:01 2006 Subject: [Histonet] (no subject) Message-ID: All, I work in the biotech industry but not with the vendor mentioned. My company ran into a similar situation in which something was not right with our product or service. We tried to contact lab supervisor to get more information but they would not answer or return our calls so we went to their boss. Our whole intention was to get more information so we could make things right but obviously the employee got in trouble for not answering our calls, posted it on the histonet and the smearing began. I noticed from the posting the other day that Joe does not like the said vendor and he most likely never will after this situation. All I ask is that you try to look at the whole picture and realize that companies, a majority of the time, do not try to get people in trouble. We just want to discuss what is wrong , how we can correct the problem and move forward. The only reason I mention this is because when it becomes my companies turn again I hope you consider that you don not know the full situation, only one side, and usually from the person upset with the company. Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not only >did I get flamed, I was burned to a crisp. I hope you are happy. You think >you may have won, but all you did was hamper a so called open forum so >people could exchange ideas and opinions. Now, many people will be afraid >to give their honest opinion for fear of retribution, Sleep tight tonight. >You almost cost me my job. Losing my job over a vendor is just not worth >it. I don't need any help, I can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of >information. I pass the baton on to someone else. It's sad that people can >not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good >luck. > > >Joe Nocito >_______________________________________________ _________________________________________________________________ Try the next generation of search with Windows Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&source=hmtagline From RCHIOVETTI <@t> aol.com Sat Oct 21 12:22:33 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Sat Oct 21 12:23:03 2006 Subject: [Histonet] Ventana Benchmark Problems Message-ID: In a message dated 10/21/2006 9:03:26 AM US Mountain Standard Time, pruegg@ihctech.net writes: > I heard from a reliable source that Danaher has made a larger bid for > Vision, not sure Ventana is in the running anymore to buy Vision. > Patsy > Yes, that's correct. It's not a "done deal" just yet, but the latest is that Danaher has made an offer, and Vision's board has unanimously made a recommendation that Danaher's offer be accepted. Danaher and Ventana had discussed a joint purchase of Vision, but that deal fell through. At that point, Ventana decided to reactivate their lawsuit against Vision. Some of you might remember that Danaher is the group that recently purchased Leica Microsystems. If the deal goes through, they'll also own Vision. You can read the press releases at Danaher's website: . Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner Southwest Precision Instruments The Desert Southwest's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representatives Member, Arizona Small Business Association - ASBA From rjbuesa <@t> yahoo.com Sat Oct 21 12:30:29 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 21 12:30:39 2006 Subject: [Histonet] Ventana Benchmark Problems In-Reply-To: Message-ID: <20061021173029.18647.qmail@web61218.mail.yahoo.com> Just "classical Ventana at its best". Either "my way or no way"; just typical. Ren? J. RCHIOVETTI@aol.com wrote: In a message dated 10/21/2006 9:03:26 AM US Mountain Standard Time, pruegg@ihctech.net writes: > I heard from a reliable source that Danaher has made a larger bid for > Vision, not sure Ventana is in the running anymore to buy Vision. > Patsy > Yes, that's correct. It's not a "done deal" just yet, but the latest is that Danaher has made an offer, and Vision's board has unanimously made a recommendation that Danaher's offer be accepted. Danaher and Ventana had discussed a joint purchase of Vision, but that deal fell through. At that point, Ventana decided to reactivate their lawsuit against Vision. Some of you might remember that Danaher is the group that recently purchased Leica Microsystems. If the deal goes through, they'll also own Vision. You can read the press releases at Danaher's website: . Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner Southwest Precision Instruments The Desert Southwest's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representatives Member, Arizona Small Business Association - ASBA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From naje1972 <@t> yahoo.com Sat Oct 21 15:58:29 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Sat Oct 21 15:58:31 2006 Subject: [Histonet] Vendors beware Message-ID: <20061021205829.96944.qmail@web33001.mail.mud.yahoo.com> Here is my 2 cents. If any vendor ever did that type of thing to me or any of my colleagues will never use any product or bi product of theirs So help me GOD. I am a small private laboratory. The customer is my boss. If said customer is dissatisfied with any of my products or services it is up to me the owner of the company to satisfy them or give them their money back. I think the company in question is underhanded and I for one will not buy or support their products or bi-products. If this company can afford to lose customers well more power to them. I am peeved. Cynthia Haynes From JWEEMS <@t> sjha.org Sun Oct 22 19:58:25 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sun Oct 22 19:58:42 2006 Subject: [Histonet] Good Bye Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE6A@sjhaexc02.sjha.org> Thank goodness, my director stood by me several years ago, when Ventana went around me to her and "sold" her on the immunostainer by touting its barcoding and offering new processors if we would convert to their system. What I said was, "Why would I want to change to more expensive immuno system to get a processor that I don't want in the first place?" She listened and they went away after quite some time of harrassing her and the pathologists. Unfortunately, they ruin a good product with behavior like this. Joe, please don't leave us. We need you. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Adams Sent: Saturday, October 21, 2006 12:22 AM To: Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From amosbrooks <@t> gmail.com Sun Oct 22 20:50:38 2006 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Oct 22 20:50:45 2006 Subject: [Histonet] Vendors Strong ArmTactics Message-ID: <582736990610221850p71b8dd56n83170125edea1cd7@mail.gmail.com> Hi, This sadly is NOT an unusual situation. You'll notice if you have ever asked a question here that you will get a lot of private messages sent directly to you not to the list in general. This is usually because they have been threatened in one way or another by a company over an opinion they have shared. This happened to me before too. A vendor that will remain nameless (that almost everyone has some equipment sold to them by these people) took issue with my stating that we were having trouble with an expensive item they sold us when someone asked about this item on the Histonet. Needless to say, I to this day do not hold the company involved in that situation in high regaurd (or any similar situation for that matter). I think the gentleman that said this provides an opportunity to solve a problem is certainly on the right track here. Rather than fixing a finishing problem with a sledge hammer, it would be better to actually deal with the problems that the user is experiencing than to try to silence them with their wretched villanous legal department. (Cease & Decist my a$$) For my part I answer questions either privately or ambiguously for purely self defensive reasons. I know that devalues this forum because we are self censoring and serving the lowest common denominator but situations like Joe's are evidence that it is just too dangerous not to be looking over our shoulder for big brother. Unfortunately some Vendors are being more of a problem than a solution. Most alarmingly it appears that the larger the company the larger the problem! Trust no-one, Amos BTW: You'll also notice I don't add where I work either. The above reasons are why. Not that it would be impossible to figure out where I work but I'm at least going to make someone have to do some homework to figure it out. From lhadley <@t> iupui.edu Mon Oct 23 06:51:28 2006 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Mon Oct 23 06:51:33 2006 Subject: [Histonet] Good Bye In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE6A@sjhaexc02.sjha.org> Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF213FE6D1@iu-mssg-mbx105.ads.iu.edu> Joyce, We had a very similar experience with said company. The techs doing the work said "NO" and they proceeded to go around, over and through us. Thank heavens we had back up and said company was asked what did the people in the lab say. It took us MONTHS to get that piece of....machinery out of our lab. Because of their practices we avoid them at all costs. Lee Ann -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Sunday, October 22, 2006 8:58 PM To: Patricia Adams; Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Thank goodness, my director stood by me several years ago, when Ventana went around me to her and "sold" her on the immunostainer by touting its barcoding and offering new processors if we would convert to their system. What I said was, "Why would I want to change to more expensive immuno system to get a processor that I don't want in the first place?" She listened and they went away after quite some time of harrassing her and the pathologists. Unfortunately, they ruin a good product with behavior like this. Joe, please don't leave us. We need you. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Adams Sent: Saturday, October 21, 2006 12:22 AM To: Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From DBotsfor <@t> wrh.on.ca Mon Oct 23 07:25:06 2006 From: DBotsfor <@t> wrh.on.ca (Botsford, Daniel) Date: Mon Oct 23 07:29:49 2006 Subject: [Histonet] Stainng Problems-Ventana -- Benchmark Message-ID: <331EE6D48DDFD51189E500508BBD360806D98468@mail.wrh.on.ca> Re Robert Bates florida Who manufactures your positive charged slide? We had ER cases where the positive control on the top of the slide stained and the a known positive patient case on the bottom half did not. Internal control did not stain. Traced the problem to slide manufactor. Manufactured failed to have an even distribution of charge on the entire slide. Use Erie Slides Sincerely Daniel Botsford Windsor Regional Hospital 1995 Lens Avenue Windsor, Ontario N8W 1L9 519-254-5577 ext 52373 519-254-6861 fax dbotsfor@wrh.on.ca From jennifer.l.hofecker <@t> Vanderbilt.Edu Mon Oct 23 08:11:47 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Mon Oct 23 08:12:02 2006 Subject: [Histonet] Goodbye Joe and other thoughts Message-ID: <898D946569A27444B65667A49C074052D0DE24@mailbe06.mc.vanderbilt.edu> Ok, I tried to stay quiet, but after two days of contemplation, I must "chime" in. First, I hope that it really isn't Good bye to Joe. I personally appreciate his humor, honesty and vast array of knowledge. He has helped me out on many occasions in the past. As for the flaming that one encounters on this list... I have (since my initiation into the flamed club) always tried to refrain from anything that could be taken as an inflammatory comment. It's not just vendors that are doing the flaming and causing trouble with employers. Most of the negativity that I have received has been by private list members. I would think that a forum such as ours can only successfully exist if we can exchange positive and negative views on topics. I admit, if I am going to post a positive endorsement for a product, I'll post to the general list. If I have a negative opinion (not often, if you know me) I will usually respond in private to the person who posted the query. Self preservation, I guess... I definitely think that going around behind people's backs to their bosses is not a good idea. I would; however, like to remind everyone that our vendor companies can also be a vehicle for tremendous good. They help out on the state, regional, and national levels with so many things that we may not even realize. It's very easy to get caught up in the negativity, but I did want to interject a little "ray of sunshine". I hope that Joe will come back to the list and I hope that those (vendors and others) who feel they must try to cause trouble over an opinion, will take a vacation, go to the spa, hibernate, or do something else to relieve their stress. As for me, I'll continue to tread lightly with my posts. Hopefully, I haven't angered anyone today. Have a great week, Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax TSH President NSH Quality Control Committee Chair From cmiller <@t> physlab.com Mon Oct 23 08:42:21 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Oct 23 08:42:34 2006 Subject: [Histonet] Good Bye In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE6A@sjhaexc02.sjha.org> Message-ID: <000601c6f6a9$10caf440$db01a8c0@plab.local> I meant to state in my last message that I personally have experienced only responsive and helpful people at Ventana. From my sales rep to the technical assistance people. That by no way excuses the treatment Joe received. Just wanted to say. Thanks, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Sunday, October 22, 2006 7:58 PM To: Patricia Adams; Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Thank goodness, my director stood by me several years ago, when Ventana went around me to her and "sold" her on the immunostainer by touting its barcoding and offering new processors if we would convert to their system. What I said was, "Why would I want to change to more expensive immuno system to get a processor that I don't want in the first place?" She listened and they went away after quite some time of harrassing her and the pathologists. Unfortunately, they ruin a good product with behavior like this. Joe, please don't leave us. We need you. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Adams Sent: Saturday, October 21, 2006 12:22 AM To: Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pmarcum <@t> vet.upenn.edu Mon Oct 23 08:49:05 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Oct 23 08:49:19 2006 Subject: [Histonet] Goodbye Joe and other thoughts In-Reply-To: <898D946569A27444B65667A49C074052D0DE24@mailbe06.mc.vanderb ilt.edu> References: <898D946569A27444B65667A49C074052D0DE24@mailbe06.mc.vanderbilt.edu> Message-ID: <6.2.5.6.2.20061023094453.01c4e7b8@vet.upenn.edu> Jennifer, Very well said and understood. I think a large number of us work under the same feelings and restraints unfortunately. Speaking freely is not acceptable unless you are making nice, nice. Joe has always strong opinions and to date had not been afraid to discuss them and most importantly he is more often right and alone in speaking out loud in public. Hope to see you sometime in the future. Pam Marcum At 09:11 AM 10/23/2006, Hofecker, Jennifer L wrote: >Ok, >I tried to stay quiet, but after two days of contemplation, I must >"chime" in. First, I hope that it really isn't Good bye to Joe. I >personally appreciate his humor, honesty and vast array of >knowledge. He has helped me out on many occasions in the past. > >As for the flaming that one encounters on this list... I have (since >my initiation into the flamed club) always tried to refrain from >anything that could be taken as an inflammatory comment. It's not >just vendors that are doing the flaming and causing trouble with >employers. Most of the negativity that I have received has been by >private list members. I would think that a forum such as ours can >only successfully exist if we can exchange positive and negative >views on topics. I admit, if I am going to post a positive >endorsement for a product, I'll post to the general list. If I have >a negative opinion (not often, if you know me) I will usually >respond in private to the person who posted the query. Self >preservation, I guess... > >I definitely think that going around behind people's backs to their >bosses is not a good idea. I would; however, like to remind >everyone that our vendor companies can also be a vehicle for >tremendous good. They help out on the state, regional, and >national levels with so many things that we may not even >realize. It's very easy to get caught up in the negativity, but I >did want to interject a little "ray of sunshine". > >I hope that Joe will come back to the list and I hope that those >(vendors and others) who feel they must try to cause trouble over an >opinion, will take a vacation, go to the spa, hibernate, or do >something else to relieve their stress. As for me, I'll continue to >tread lightly with my posts. Hopefully, I haven't angered anyone today. > >Have a great week, >Jennifer >Jennifer Hofecker, HT (ASCP) >Vanderbilt University Medical Center >Division of Neuropathology >(615) 343-0083 >(615) 343-7089 fax >TSH President >NSH Quality Control Committee Chair > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From rjbuesa <@t> yahoo.com Mon Oct 23 09:09:43 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 23 09:09:58 2006 Subject: [Histonet] Good Bye In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE6A@sjhaexc02.sjha.org> Message-ID: <20061023140943.28167.qmail@web61213.mail.yahoo.com> Joyce: Yours is not an isolated case. Ventana's sales practices involve the following behaviours: 1- go above the knowledgeable people to the managers to "pull the rug" in front of their eyes; 2- offer things they cannot deliver; 3- conceal that their products are unnecessarily overpriced and that their instruments are essentially a "closed" system; 4- conceal also that their "famous liquid coverslip", imprescindible to complete their staining protocols, is nothing more than low viscosity mineral oil and because of that you have to use their Abs diluted in it; 5- and push, push, push until the nauseating level. That is why I do NOT like Ventana. Ren? J. NOTE: I am retired, and they cannot "touch" me (HA, HA, HA) "Weems, Joyce" wrote: Thank goodness, my director stood by me several years ago, when Ventana went around me to her and "sold" her on the immunostainer by touting its barcoding and offering new processors if we would convert to their system. What I said was, "Why would I want to change to more expensive immuno system to get a processor that I don't want in the first place?" She listened and they went away after quite some time of harrassing her and the pathologists. Unfortunately, they ruin a good product with behavior like this. Joe, please don't leave us. We need you. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Adams Sent: Saturday, October 21, 2006 12:22 AM To: Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From rjbuesa <@t> yahoo.com Mon Oct 23 09:17:59 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 23 09:18:11 2006 Subject: [Histonet] Just a thought! Message-ID: <20061023141759.80962.qmail@web61224.mail.yahoo.com> Why all of you so rightly outraged by what has happened to Joe Nocito because of the UNJUSTIFIED complaint by a vendor do not take this issue to your lab manager as an example of what it should not be done? Use also the opportunity to explain to your manager the great usefulness of Histonet and that when you have a problem you expect to receive an honest answer, not hampered by this type of retaliation. Private answers, although end being useful to the recipient, preclude the information to reach to all subscribers. I believe that Linda Margraff should also take a look into this problem that affects Histonet. Just a thoght! Ren? J. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From er.lang <@t> gmx.at Mon Oct 23 09:19:29 2006 From: er.lang <@t> gmx.at (Erich Lang) Date: Mon Oct 23 09:19:14 2006 Subject: [Histonet] protocol for PTEN on Benchmark XT Message-ID: <000101c6f6ae$4148cbb0$c812a8c0@dielangs.at> Hi, can someone provide me with a working procedure for the Dako PTEN-Antibody on the Ventana Benchmark XT (ultra-kit)? I also need some good advice for the joice of positive control. Thank you Gudrun Lang From JMitchell <@t> uwhealth.org Mon Oct 23 09:28:55 2006 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Mon Oct 23 09:29:02 2006 Subject: [Histonet] Goodbye Joe and other thoughts In-Reply-To: <898D946569A27444B65667A49C074052D0DE24@mailbe06.mc.vanderbilt.edu> Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB10225AA12@uwhis-xchng4.uwhis.hosp.wisc.edu> In the original histonet "goodbye" post from Friday 10/20/06 it states: "some one called my place of employment". A company name is not specifically mentioned as being the caller but conclusions are being drawn that it was a particular vendor that made the phone call. I am not necessarily defending a company/vendor here (I do not use their product) but.....that phone call may very well have been from an individual histonet member who made the contact. We do not know and in all fairness it cannot be assumed and conclusions should not be made that the particular company in question made the call to Joe's employer. Thanks Jennifer for bringing up some good thoughts on this! Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI 53792 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: Monday, October 23, 2006 8:12 AM To: histonet; Nocito, Joe Subject: [Histonet] Goodbye Joe and other thoughts Ok, I tried to stay quiet, but after two days of contemplation, I must "chime" in. First, I hope that it really isn't Good bye to Joe. I personally appreciate his humor, honesty and vast array of knowledge. He has helped me out on many occasions in the past. As for the flaming that one encounters on this list... I have (since my initiation into the flamed club) always tried to refrain from anything that could be taken as an inflammatory comment. It's not just vendors that are doing the flaming and causing trouble with employers. Most of the negativity that I have received has been by private list members. I would think that a forum such as ours can only successfully exist if we can exchange positive and negative views on topics. I admit, if I am going to post a positive endorsement for a product, I'll post to the general list. If I have a negative opinion (not often, if you know me) I will usually respond in private to the person who posted the query. Self preservation, I guess... I definitely think that going around behind people's backs to their bosses is not a good idea. I would; however, like to remind everyone that our vendor companies can also be a vehicle for tremendous good. They help out on the state, regional, and national levels with so many things that we may not even realize. It's very easy to get caught up in the negativity, but I did want to interject a little "ray of sunshine". I hope that Joe will come back to the list and I hope that those (vendors and others) who feel they must try to cause trouble over an opinion, will take a vacation, go to the spa, hibernate, or do something else to relieve their stress. As for me, I'll continue to tread lightly with my posts. Hopefully, I haven't angered anyone today. Have a great week, Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax TSH President NSH Quality Control Committee Chair _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Mon Oct 23 09:46:00 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Oct 23 09:41:41 2006 Subject: [Histonet] Goodbye Joe and other thoughts Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C2C@LIL.xRothGen.nhs.uk> Can someone summarise what has happened in a few sentences, as I have not been following the thread. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Mitchell Jean A. [mailto:JMitchell@uwhealth.org] Sent: 23 October 2006 15:29 To: Hofecker, Jennifer L; histonet Subject: RE: [Histonet] Goodbye Joe and other thoughts In the original histonet "goodbye" post from Friday 10/20/06 it states: "some one called my place of employment". A company name is not specifically mentioned as being the caller but conclusions are being drawn that it was a particular vendor that made the phone call. I am not necessarily defending a company/vendor here (I do not use their product) but.....that phone call may very well have been from an individual histonet member who made the contact. We do not know and in all fairness it cannot be assumed and conclusions should not be made that the particular company in question made the call to Joe's employer. Thanks Jennifer for bringing up some good thoughts on this! Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI 53792 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: Monday, October 23, 2006 8:12 AM To: histonet; Nocito, Joe Subject: [Histonet] Goodbye Joe and other thoughts Ok, I tried to stay quiet, but after two days of contemplation, I must "chime" in. First, I hope that it really isn't Good bye to Joe. I personally appreciate his humor, honesty and vast array of knowledge. He has helped me out on many occasions in the past. As for the flaming that one encounters on this list... I have (since my initiation into the flamed club) always tried to refrain from anything that could be taken as an inflammatory comment. It's not just vendors that are doing the flaming and causing trouble with employers. Most of the negativity that I have received has been by private list members. I would think that a forum such as ours can only successfully exist if we can exchange positive and negative views on topics. I admit, if I am going to post a positive endorsement for a product, I'll post to the general list. If I have a negative opinion (not often, if you know me) I will usually respond in private to the person who posted the query. Self preservation, I guess... I definitely think that going around behind people's backs to their bosses is not a good idea. I would; however, like to remind everyone that our vendor companies can also be a vehicle for tremendous good. They help out on the state, regional, and national levels with so many things that we may not even realize. It's very easy to get caught up in the negativity, but I did want to interject a little "ray of sunshine". I hope that Joe will come back to the list and I hope that those (vendors and others) who feel they must try to cause trouble over an opinion, will take a vacation, go to the spa, hibernate, or do something else to relieve their stress. As for me, I'll continue to tread lightly with my posts. Hopefully, I haven't angered anyone today. Have a great week, Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax TSH President NSH Quality Control Committee Chair _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Mon Oct 23 09:45:57 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Mon Oct 23 09:46:11 2006 Subject: [Histonet] Good Bye Message-ID: How can someone be "burned at the stake" for using OUR "their own" website? The HistoNet is NOT for VENDORS!!! Is that not the way this was set up. I really thought they were not supposed to be on here at all. Do we no longer have free speech because of these companies that are not supposed to be on here!!!!! Please correct me if I am wrong....maybe I don't get it .... but this sounds insane. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, October 20, 2006 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From JWEEMS <@t> sjha.org Mon Oct 23 09:49:07 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Oct 23 09:49:32 2006 Subject: [Histonet] Good Bye Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEE80@sjhaexc02.sjha.org> The saddest part is that the employer would listen. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Carole Fields Sent: Monday, October 23, 2006 10:46 AM To: 'Joe Nocito' Cc: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Good Bye How can someone be "burned at the stake" for using OUR "their own" website? The HistoNet is NOT for VENDORS!!! Is that not the way this was set up. I really thought they were not supposed to be on here at all. Do we no longer have free speech because of these companies that are not supposed to be on here!!!!! Please correct me if I am wrong....maybe I don't get it .... but this sounds insane. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, October 20, 2006 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Bye Just as I expected, some one called my place of employment. Not only did I get flamed, I was burned to a crisp. I hope you are happy. You think you may have won, but all you did was hamper a so called open forum so people could exchange ideas and opinions. Now, many people will be afraid to give their honest opinion for fear of retribution, Sleep tight tonight. You almost cost me my job. Losing my job over a vendor is just not worth it. I don't need any help, I can do it just fine by myself. Netters, it's been fun. I had many laughs and received a lot of information. I pass the baton on to someone else. It's sad that people can not give their honest opinion. We'll be meeting again, you can be sure of that. Good bye and good luck. Joe Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Janet.Bonner <@t> FLHOSP.ORG Mon Oct 23 10:00:11 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Oct 23 10:01:26 2006 Subject: [Histonet] Good Bye References: <20061023140943.28167.qmail@web61213.mail.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A657@fhosxchmb006.ADVENTISTCORP.NET> Too bad vendors can't be banned from the List. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Mon 10/23/2006 10:09 AM To: Weems, Joyce; Patricia Adams; Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Joyce: Yours is not an isolated case. Ventana's sales practices involve the following behaviours: 1- go above the knowledgeable people to the managers to "pull the rug" in front of their eyes; 2- offer things they cannot deliver; 3- conceal that their products are unnecessarily overpriced and that their instruments are essentially a "closed" system; 4- conceal also that their "famous liquid coverslip", imprescindible to complete their staining protocols, is nothing more than low viscosity mineral oil and because of that you have to use their Abs diluted in it; 5- and push, push, push until the nauseating level. That is why I do NOT like Ventana. Ren? J. NOTE: I am retired, and they cannot "touch" me (HA, HA, HA) "Weems, Joyce" wrote: Thank goodness, my director stood by me several years ago, when Ventana went around me to her and "sold" her on the immunostainer by touting its barcoding and offering new processors if we would convert to their system. What I said was, "Why would I want to change to more expensive immuno system to get a processor that I don't want in the first place?" She listened and they went away after quite some time of harrassing her and the pathologists. Unfortunately, they ruin a good product with behavior like this. Joe, please don't leave us. We need you. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Adams Sent: Saturday, October 21, 2006 12:22 AM To: Tarango, Mark; HistoNet Subject: RE: [Histonet] Good Bye Dear All, I normally just read what is going on in the world of Histonetters and do not write, but I think that Joe's Employers should have stuck by him before taking the Vendors side. Just by reading all of his good advice over this last year, I have found that he has great judgment. I think that the Bosses should have considered that and told the Vendor that maybe there is a good reason for Joe's dissatisfaction. I have left a job where one person can cause problems and my side was never asked for my Bosses blindly believed a jealous person. So maybe bosses should value their employees and stand by them, the Vendor is not doing the work for them after all, Joe is. By the way I moved on to a job where having a brain and the knowledge to trouble shoot and get the work done is not taken for granted. I am very valued for what I can do. As we all should be valued, we do great work and have an important place in Health Care, be it Human or Animal. So keep up your spirit and fire Joe. I would be proud to stand by you, and not behind you! Patricia Adams, HT (ASCP) Supervisor Histology College of Veterinary Medicine and Allied Health Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Vickroy.Jim <@t> mhsil.com Mon Oct 23 10:06:45 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Oct 23 10:07:05 2006 Subject: [Histonet] CAP QUESTION Message-ID: Does anybody have an explanation of what it means by direct vs indirect supervision of a pathologist for non-pathologists performing gross descriptions? I realize you have to document what type of specimens that non-pathologists are grossing but have always wondered what the difference is between direct and indirect. I would appreciate any feedback of others on how they have interpreted this question. Question - ANP.11630 Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From rjbuesa <@t> yahoo.com Mon Oct 23 10:09:22 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 23 10:09:36 2006 Subject: [Histonet] A complex issue. Message-ID: <20061023150923.48617.qmail@web61220.mail.yahoo.com> Dear Dr. Margraf: If you have monitored Histonet in the last 3 days you may have found that, because some comments he did, Mr. Joe Nocito who is a frequent and useful contributor to Histonet, was reprimanded by his boss after a vendor from Ventana complained about Joe's comments. Free speach is involved in this incident: a- Joe's right to express his opinion; and b- the Ventana's representative to complain about it. The reprimand was an internal issue and perhaps motivated by some internal administrative regulation and may be justified, but the use of Histonet as an spying tool by a vendor, I don't think is right. Very rightly so you have regulated the use of Histonet and have requested that vendors do not use it as a sales vehicle (although some do it from time to time) and although I do not think that you can do much about this incident I wanted to let you know that Histonet has been used as an spying and retaliatory vehicle by a vendor that, instead of taking the complaint to improve a product or a service, has opted to use for mean purposes hiden in anonymity! Ren? J. Buesa --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From HornHV <@t> archildrens.org Mon Oct 23 10:21:21 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Oct 23 10:21:53 2006 Subject: [Histonet] ventanna what do you have to say to this Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6E39@EMAIL.archildrens.org> I'm just wondering why Ventanna is not responding. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Ebreisch <@t> chsd.org Mon Oct 23 11:04:31 2006 From: Ebreisch <@t> chsd.org (Breisch, Eric) Date: Mon Oct 23 11:04:31 2006 Subject: [Histonet] CAP question regarding annual testing of microwave Message-ID: Hi Histologists, I have a general inquiry regarding a CAP question. The question concerns the annual monitoring of microwave radiation emitted from the laboratory microwave. This is something we have never checked and when we addressed this with our biomedical staff they didn't have an answer as to how that would be done. Could someone please share how that would be monitored and what equipment would be necessary for that task? Thank you, Eric A. Breisch Department of Pathology Rady Children's Hospital San Diego, CA From tbraud <@t> holyredeemer.com Mon Oct 23 11:10:43 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Oct 23 11:12:25 2006 Subject: [Histonet] CAP QUESTION In-Reply-To: Message-ID: Hi Jim - Direct Supervision is where a pathologist would be standing over your shoulder watching you actually gross the specimen. Indirect Supervision is where he/she would review the gross, perhaps in the written report, though the pathologist did not actually observe you performing the actual gross dissection. Hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Monday, October 23, 2006 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP QUESTION Does anybody have an explanation of what it means by direct vs indirect supervision of a pathologist for non-pathologists performing gross descriptions? I realize you have to document what type of specimens that non-pathologists are grossing but have always wondered what the difference is between direct and indirect. I would appreciate any feedback of others on how they have interpreted this question. Question - ANP.11630 Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From ander093 <@t> tc.umn.edu Mon Oct 23 11:15:44 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Mon Oct 23 11:16:06 2006 Subject: [Histonet] Good Bye In-Reply-To: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> References: <000801c6f480$754d9dd0$066ace44@yourxhtr8hvc4p> Message-ID: <6.2.3.4.0.20061023111331.01dc1f98@ander093.email.umn.edu> Joe, I would say that it is your companies loss and that the vender in question probably hasn't done themselves any good here!! Good luck to you--with your knowledge and experience, you should be able to find a better position in no time, hopefully with a company that will value your opinion. LuAnn At 02:46 PM 10/20/2006, Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not > only did I get flamed, I was burned to a crisp. I hope you are > happy. You think you may have won, but all you did was hamper a so > called open forum so people could exchange ideas and opinions. Now, > many people will be afraid to give their honest opinion for fear of > retribution, Sleep tight tonight. You almost cost me my job. Losing > my job over a vendor is just not worth it. I don't need any help, I > can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that > people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > >Joe Nocito >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gamal.akabani <@t> gmail.com Mon Oct 23 11:16:29 2006 From: gamal.akabani <@t> gmail.com (Gamal Akabani) Date: Mon Oct 23 11:16:42 2006 Subject: [Histonet] My opinion to this issue Message-ID: I am not a Histonet by profession but I follow your comments and advice in the many issues you post. This site is extremely important and should not be tarnished by the actions of a vendor. The issue has no basis for retribution. 1.- Joe's comments were posted in an open space for ALL to see and comment. His comments were not irrational nor based on prejudice. 2.- Joe's opinion is protected and cannot be punished by ANY academic system as it was OPEN to all to see and comment. Internal or no internal regulation, retribution is against the law regardless of the situation. 3.- Vendors are welcomed to this site as long as they base their opinions and comments on a scientific basis, no propaganda etc, no personal attacks. 4.- A formal letter or e-mail from the members of this site should be sent to Ventana and advice them that such actions do have repercussions and shall not be tolerated in the future. Our academic freedom is fundamental to our work, a principle that cannot and shall not be hinder on the basis of prejudice or disagreement with a vendor or academic administration for that matter. I do not think Ventana would like to see an open letter to the editor of an important journal mentioning this issue for all of us to see and ponder. -- Gamal Akabani From rjbuesa <@t> yahoo.com Mon Oct 23 11:27:14 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 23 11:27:29 2006 Subject: [Histonet] CAP QUESTION In-Reply-To: Message-ID: <20061023162714.85704.qmail@web61211.mail.yahoo.com> Jim: Direct supervision is when the pathologist is "looking over your shoulder" and indirect is when the pathologist has delegated that function on a trained supervisor, and the supervisor is now the one "looking over your shoulder" (in a figurative speach). Ren? J. "Vickroy, Jim" wrote: Does anybody have an explanation of what it means by direct vs indirect supervision of a pathologist for non-pathologists performing gross descriptions? I realize you have to document what type of specimens that non-pathologists are grossing but have always wondered what the difference is between direct and indirect. I would appreciate any feedback of others on how they have interpreted this question. Question - ANP.11630 Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From Terry.Marshall <@t> rothgen.nhs.uk Mon Oct 23 11:27:12 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Oct 23 11:32:19 2006 Subject: [Histonet] Good Bye Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C30@LIL.xRothGen.nhs.uk> Pam Marcum has kindly sent me an off-list summary. I urge you all to not allow people to use the list for spying and tell-tail-titting. This would be the death knoll for opinions on equipment, which forms a substantial proportion of the discussion. Anyone so doing should be ostracised to the full extent possible. It is despicable. Mama don't allow...... Joe - stick with us. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: LuAnn Anderson [mailto:ander093@tc.umn.edu] Sent: 23 October 2006 17:16 To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Good Bye Joe, I would say that it is your companies loss and that the vender in question probably hasn't done themselves any good here!! Good luck to you--with your knowledge and experience, you should be able to find a better position in no time, hopefully with a company that will value your opinion. LuAnn At 02:46 PM 10/20/2006, Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not > only did I get flamed, I was burned to a crisp. I hope you are > happy. You think you may have won, but all you did was hamper a so > called open forum so people could exchange ideas and opinions. Now, > many people will be afraid to give their honest opinion for fear of > retribution, Sleep tight tonight. You almost cost me my job. Losing > my job over a vendor is just not worth it. I don't need any help, I > can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that > people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > >Joe Nocito >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Mon Oct 23 11:43:47 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Mon Oct 23 11:44:01 2006 Subject: [Histonet] CAP question regarding annual testing of microwave In-Reply-To: References: Message-ID: <453CF143.4070603@ebsciences.com> Hope you don't mind a "vendor" response: Check out http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf As for purchase of a leakage detector, I'm not going to try and sell you one in this non-commercial forum. However, leakage detectors are available in a wide range of prices; I would avoid the "idiot light" type that does not give you an actual measurement, both because you should log actual readings, and because they are prone to false positives. Also I'd avoid passive (non-powered) devices since they can be inaccurate; very expensive equipment might not be advisable either, since CAP might get more specific with their leakage detection requirements in the future. It's better to have to discard a less-expensive equipment in that eventuality. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" You must be the change you want to see in the world. - Mahatma Gandhi > Hi Histologists, > I have a general inquiry regarding a CAP question. The question > concerns the annual monitoring of microwave radiation emitted from the > laboratory microwave. This is something we have never checked and when we > addressed this with our biomedical staff they didn't have an answer as to > how that would be done. Could someone please share how that would be > monitored and what equipment would be necessary for that task? > > Thank you, > > Eric A. Breisch > Department of Pathology > Rady Children's Hospital > San Diego, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" You must be the change you want to see in the world. - Mahatma Gandhi From rjbuesa <@t> yahoo.com Mon Oct 23 11:43:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 23 11:44:05 2006 Subject: [Histonet] CAP question regarding annual testing of microwave In-Reply-To: Message-ID: <20061023164352.47365.qmail@web61215.mail.yahoo.com> Eric: You would have to calibrate your microwave oven once a year to determine if the operation wattage remains constant year to year, or what is the variation, if any. Ren? J. "Breisch, Eric" wrote: Hi Histologists, I have a general inquiry regarding a CAP question. The question concerns the annual monitoring of microwave radiation emitted from the laboratory microwave. This is something we have never checked and when we addressed this with our biomedical staff they didn't have an answer as to how that would be done. Could someone please share how that would be monitored and what equipment would be necessary for that task? Thank you, Eric A. Breisch Department of Pathology Rady Children's Hospital San Diego, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From Tbarnhart <@t> primecare.org Mon Oct 23 12:13:11 2006 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Mon Oct 23 12:13:28 2006 Subject: [Histonet] CAP question regarding annual testing of microwave Message-ID: <1779904B5E82D511914C00D0B79333920E48E9CA@exchangent> I interpreted this to mean a "leakage" test for our microwaves. I contacted a local small appliance repair shop, had them do a leakage monitor and record/signoff on the results. It was very cheap and I didn't have to purchase a leakage monitor. Tammy Barnhart, AP Supervisor St. Alexius Medical Center Bismarck, ND -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breisch, Eric Sent: Monday, October 23, 2006 11:05 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CAP question regarding annual testing of microwave Hi Histologists, I have a general inquiry regarding a CAP question. The question concerns the annual monitoring of microwave radiation emitted from the laboratory microwave. This is something we have never checked and when we addressed this with our biomedical staff they didn't have an answer as to how that would be done. Could someone please share how that would be monitored and what equipment would be necessary for that task? Thank you, Eric A. Breisch Department of Pathology Rady Children's Hospital San Diego, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From Erin.Wrona <@t> kp.org Mon Oct 23 12:03:26 2006 From: Erin.Wrona <@t> kp.org (Erin.Wrona@kp.org) Date: Mon Oct 23 12:20:30 2006 Subject: [Histonet] Re: goodbye Message-ID: Amos wrote: "This happened to me before too. A vendor that will remain nameless (that almost everyone has some equipment sold to them by these people) took issue with my stating that we were having trouble with an expensive item they sold us when someone asked about this item on the Histonet." I am sorry to say that this happened to me as well at a previous job. Since then I am always VERY careful about what I say (not much after the incident) about any product. It's sad but sales reps seem to be rather aggressive in acting to silence criticism of their (extremely expensive) products. -Erin NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From Janet.Bonner <@t> FLHOSP.ORG Mon Oct 23 12:16:14 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Oct 23 12:20:32 2006 Subject: [Histonet] My opinion to this issue References: Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A65D@fhosxchmb006.ADVENTISTCORP.NET> Very well said! I agree! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gamal Akabani Sent: Mon 10/23/2006 12:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] My opinion to this issue I am not a Histonet by profession but I follow your comments and advice in the many issues you post. This site is extremely important and should not be tarnished by the actions of a vendor. The issue has no basis for retribution. 1.- Joe's comments were posted in an open space for ALL to see and comment. His comments were not irrational nor based on prejudice. 2.- Joe's opinion is protected and cannot be punished by ANY academic system as it was OPEN to all to see and comment. Internal or no internal regulation, retribution is against the law regardless of the situation. 3.- Vendors are welcomed to this site as long as they base their opinions and comments on a scientific basis, no propaganda etc, no personal attacks. 4.- A formal letter or e-mail from the members of this site should be sent to Ventana and advice them that such actions do have repercussions and shall not be tolerated in the future. Our academic freedom is fundamental to our work, a principle that cannot and shall not be hinder on the basis of prejudice or disagreement with a vendor or academic administration for that matter. I do not think Ventana would like to see an open letter to the editor of an important journal mentioning this issue for all of us to see and ponder. -- Gamal Akabani _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From 1dpeterson <@t> meriter.com Mon Oct 23 12:25:55 2006 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Mon Oct 23 12:26:06 2006 Subject: [Histonet] Cryostats Message-ID: <328CBAE62F31C642B422970E879DFADC01A80133@pcwex01> Hello Histonetters! I'm currently looking to replace our relic cryostats and am wondering if any of you are using the Shandon Cryotome. We are in a clinical setting, doing about 5 frozens (I know - whew) a day. Sometimes we may get all the way up to 10!!! We even get our Pathologists to cut them!! Anyway, any input you can give me pro or con regarding this particular cryostat (or any other for that matter) will be greatly appreciated. Feel free to contact me privately if you feel the need to avoid any backlash. Thanks in advance Dan Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From Kari.Zajic <@t> HCAhealthcare.com Mon Oct 23 12:38:18 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Mon Oct 23 15:46:32 2006 Subject: [Histonet] Cryostat decontamination policy Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC787@ORLEV03.hca.corpad.net> Hello all, would anyone be willing to share their cryostat decontamination policy? We do not have a UV cryostat (we have a Leica 1850) and were wondering if anyone had a procedure to disinfect without defrosting? Thank you in advance... Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From petepath <@t> yahoo.com Mon Oct 23 12:39:46 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Mon Oct 23 15:46:35 2006 Subject: [Histonet] Lymph Node Frozen Sections Message-ID: <20061023173946.36127.qmail@web30407.mail.mud.yahoo.com> Dear Travis, For lymphnode frozens first remove all fat from the outer surface. When given the usual fatty wad I place it on a paper towel an first using mild pressure with my finget tips I sqeeze away all the fat I can by pushing it away from the node. It should look like a recognizable oval or bean shaped node by now. Next lie the node on a dry paper towel. Roll it a few mm. As the node rolls the fatty tissue adherent to the capsule sticks to the paper and a plane of dissection which opens as the node rolls away. With a scalpel slice the capsular fat away as this plane open. Continue rolling and slicing until the capsule is clean. If you leave fat on the capsule when you go to cut it will separate from the OCT. If fat totally surrounds the capsule the tissue will entirely" curl away" from the OCT and make you miserable. When you bivalve the node there will often be fat in the medulla. I will scrape out as much of this as I can with a scalpel. If you have done a good job this the lymph node will cut as easy as any other tissue. In general try to cut in a smooth continuous fashion and always start with a sharp blade. If you do this there may be a few holes where fat remains but the nodal tissue will cut nicely. If you are still having difficulty with some fat in the block you can scrape it away from the block face and then apply a drop of OCT to the block face again an press it to the cold stage to finish the "plastering" job. On my web site I call this "The Gouge" Few other words on fat. If possiblett try to have the fat hit the blade last or by itself. Always give yourself a " handle of OCT" that will hit the blade before the fat.The worse thing you can do is have fat hit the blade first without cutting through some OCT first. Unless the tissue is super cold it will just smear everything in its path. To learn about continuous cutting, fat tricks and general frozen section technique visit my frozen section tutorial at: http://pathologyinnovations.com/frozen_section_technique.htm Good luck, Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From babak.ghaednia <@t> gmail.com Tue Oct 10 03:45:05 2006 From: babak.ghaednia <@t> gmail.com (babak ghaednia) Date: Mon Oct 23 15:46:38 2006 Subject: [Histonet] May-Grunwald Staining Message-ID: <6a0156b90610100145x34ddc4aah2055020d8e61fdec@mail.gmail.com> Dear sir I am babak, head of infectious shrimp diseases, Iran Shrimp Research Center, ISRC. I would like to ask for your help regarding the procedure on making May-Grunwald stock solution from its powder form. Any immediate assistance will be very much appreciated! Thank you so much! Respectfully yours, Babak Gaednia From %UPI% <@t> ec.auckland.ac.nz Thu Oct 12 16:15:25 2006 From: %UPI% <@t> ec.auckland.ac.nz (%name%) Date: Mon Oct 23 15:46:41 2006 Subject: [Histonet] cryosectioning calcified bone on manual freezing sledging microtome ? Message-ID: <452EB06D.2070509@ec.auckland.ac.nz> In my research project I am using Differential Interference Contrast (DIC) Microscopy to look at the attachment of the intervertebral disc to the vertebral body. To date I have been fixing (Formalin) and decalcifying (Formic Acid) the bone-disc-bone samples (approx 5mm by 10mm at the largest, of annulus and bone) before cutting 25-40micron thick slices on a manual Freezing Sledging Microtome with disposable steel blades (the sample is frozen onto a sample block with OCT using liquid nitrogen). For the next series of experiments I need fresh/hydrated slices of a similar thickness that haven't been fixed or decalcified. I have access to a tungsten carbide D profile microtome knife that I thought may work (I have heard of other researchers cutting undecalcified bone set in resin with tungsten-carbide blades). My attempts to date at using this knife on the freezing sledging microtome have been unsuccessful. I was wondering if; A) the concept of cutting undecalcifed bone on a manual freezing sledging microtome is fundamentally flawed (I've only been able to find comments on cryo-sectioning undecalcifed bone on a cryostat). Is there a different type of microtome that would be better and still allow me to avoid both resins and decalcification ? B) the blade could be blunt (I'm not sure of it's history - previous user not the university anymore). It looks ok to me under the microscope (but I'm no expert!). Should I be able to tell under a microscope ? I would appreciate any advise. Regards Meredith Schollum Bio-Materials Lab University of Auckland New Zealand From acjanes <@t> bu.edu Mon Oct 23 13:00:04 2006 From: acjanes <@t> bu.edu (acjanes@bu.edu) Date: Mon Oct 23 15:47:09 2006 Subject: [Histonet] Flash Freezing Brain Message-ID: <20061023140004.kew9yf6xww488w4s@www.bu.edu> I have a question about flash freezing rodent brains, as sometimes I see little holes destroying my tissue and I cannot determine the cause. Normally I perfuse mice with 4% para, post fix for 1-4 hours with para at room temp then put in 30% sucrose until the brain drops (At 4C). After this I make an foil mold which I fill with OCT and put my brain inside. I then slowly lower the foil/brain mold into a bath containing isopentane and dry ice just until everything freezes. I then store the tissue at -20 until ready to cut. Normally this works, but occasionally I loose tissue to the holes. I am now going to be starting a project using rats and I want to make sure all the tissue stays intact. Does anyone have suggestions? We need the tissue to run ICC for c-fos the brains will be cut at 40 microns using a cryostat. From JMahoney <@t> alegent.org Mon Oct 23 14:16:01 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Mon Oct 23 15:48:29 2006 Subject: [Histonet] Good Bye Message-ID: Joe said this situation "almost cost him his job". As a manager I can't imagine an employer firing someone for stating an opinion about a product or methodology. (Now, if Joe were spending all his time on the net and not doing his job, that would be a different story. Those of us who keep up on this net know Joe to be a very dedicated professional so I doubt that is the case.) We are all in this together and as far as I'm concerned it is about one thing; PATIENT CARE. It does not matter if you are in research or in a clinical lab, it is all about the patients. Any time a one of us says anything negative about a product it is because we are striving for quality patient care. It is like I tell my staff; We have to leave our egos outside the lab. If your quality is not up to snuff it is not about you, it is about the patient. My point to the vendors is this; If we are not happy about your products leave your ego outside. We want products that will help us deliver quality slides. That is all that matters. Take the criticism and make your products better or more consistent. Enough, I'll get off my soap box. The above opinions do not reflect those of my employer, boss, dog or maybe even me. Jan Omaha >>> LuAnn Anderson 10/23/2006 11:15 AM >>> Joe, I would say that it is your companies loss and that the vender in question probably hasn't done themselves any good here!! Good luck to you--with your knowledge and experience, you should be able to find a better position in no time, hopefully with a company that will value your opinion. LuAnn At 02:46 PM 10/20/2006, Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not > only did I get flamed, I was burned to a crisp. I hope you are > happy. You think you may have won, but all you did was hamper a so > called open forum so people could exchange ideas and opinions. Now, > many people will be afraid to give their honest opinion for fear of > retribution, Sleep tight tonight. You almost cost me my job. Losing > my job over a vendor is just not worth it. I don't need any help, I > can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that > people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > >Joe Nocito >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjenkins <@t> sch-farmville.org Mon Oct 23 14:28:39 2006 From: jjenkins <@t> sch-farmville.org (Jennie Jenkins) Date: Mon Oct 23 15:48:31 2006 Subject: [Histonet] PA Programs available? Message-ID: <453D17E7.2030808@sch-farmville.org> Does anyone out there know if there are any PA programs that offer online coursework so that I would only have to travel for the labs? Or is there anyone out there who has sit for the exam through the OJT route and could shed some light. I already know the requirements, I just don't know if I can pass the exam based on the fact that we do such limited variety of specimens in our hospital. Thanks in advance. Jennie Jenkins, MT Southside Community Hospital Farmville, VA 23901 434-315-2618 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== From NMargaryan <@t> childrensmemorial.org Mon Oct 23 14:45:25 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Oct 23 15:48:34 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 39 Message-ID: <63B8B599DE283148B92E83C78B32C15D03258333@cmhexbe2.childrensmemorial.org> Very well said! I agree! Naira Message: 3 Date: Mon, 23 Oct 2006 13:16:14 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] My opinion to this issue To: "Gamal Akabani" , Histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A65D@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 Very well said! I agree! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gamal Akabani Sent: Mon 10/23/2006 12:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] My opinion to this issue I am not a Histonet by profession but I follow your comments and advice in the many issues you post. This site is extremely important and should not be tarnished by the actions of a vendor. The issue has no basis for retribution. 1.- Joe's comments were posted in an open space for ALL to see and comment. His comments were not irrational nor based on prejudice. 2.- Joe's opinion is protected and cannot be punished by ANY academic system as it was OPEN to all to see and comment. Internal or no internal regulation, retribution is against the law regardless of the situation. 3.- Vendors are welcomed to this site as long as they base their opinions and comments on a scientific basis, no propaganda etc, no personal attacks. 4.- A formal letter or e-mail from the members of this site should be sent to Ventana and advice them that such actions do have repercussions and shall not be tolerated in the future. Our academic freedom is fundamental to our work, a principle that cannot and shall not be hinder on the basis of prejudice or disagreement with a vendor or academic administration for that matter. I do not think Ventana would like to see an open letter to the editor of an important journal mentioning this issue for all of us to see and ponder. -- Gamal Akabani _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 39 **************************************** From JWEEMS <@t> sjha.org Mon Oct 23 14:42:30 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Oct 23 15:48:37 2006 Subject: [Histonet] Computer Orders for Pathology Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEEA6@sjhaexc02.sjha.org> You guys were great to share info with me from my recent workload questions. Thanks so much. Another one... How many of you are no longer using paper requisitions? We have been requested to eliminate ours and to order through our IDX system. If anyone does this, would you please share your experience? It seems to me we don't have enough spaces to include the info our pathologists need, but I may have blinders on. I'd appreciate your experience and how it has worked for you. We seem to have a hard time getting paper requisitions completed, not sure how going paperless could work! Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ASenn <@t> mercy.pmhs.org Mon Oct 23 14:59:54 2006 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Mon Oct 23 15:48:52 2006 Subject: [Histonet] Private emails Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A5C@pmhs2kxch03> Subject: [Histonet] Vendors Strong ArmTactics To: histonet@lists.utsouthwestern.edu Message-ID: <582736990610221850p71b8dd56n83170125edea1cd7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi, This sadly is NOT an unusual situation. You'll notice if you have ever asked a question here that you will get a lot of private messages sent directly to you not to the list in general. This is usually because they have been threatened in one way or another by a company over an opinion they have shared. ***************Amos, I agree with you. I've asked several questions that were answered via private email. I always wondered why....but I guess this is reason enough. It's sad that we are supposed to learn and grow from this list and because of what happened with Joe, information isn't being shared.... I wonder why the vendor has nothing to say. Amy Senn - Ignored: ps--good to hear from you Amos :) From algranth <@t> u.arizona.edu Mon Oct 23 15:09:46 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Oct 23 15:49:12 2006 Subject: [Histonet] Re: goodbye In-Reply-To: Message-ID: <4.3.2.7.2.20061023125201.00cc6480@algranth.inbox.email.arizona.edu> It happened to me also - maybe this happens more than we think??? I was chastised by a vendor when I asked if anybody else was experiencing a certain phenomenon with one of their products. He told me that I should have gone to them instead of asking my question on histonet. Well, I did and really didn't get any help from them when I did this before going to histonet. Instead they were trying to tell me to change my technique. I don't know about you, but after you do something one way for 25 or more years it would seem that it wouldn't be the technique - or not usually. Long story short, we danced around this problem until somebody finally told me they had a bad batch of base "product" that went into certain lots. Bingo! Thank God for the brave soul that finally admitted that the company did have a problem but why couldn't they admit it weeks sooner and save me the grief that I had with my tissues? By the way, there were others out in histoland having the same problem. Hope they got it fixed too. Andi At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: >Amos wrote: "This happened to me before too. A vendor that will remain >nameless >(that almost everyone has some equipment sold to them by these people) >took >issue with my stating that we were having trouble with an expensive item >they sold us when someone asked about this item on the Histonet." > >I am sorry to say that this happened to me as well at a previous job. >Since then I am always VERY careful about what I say (not much after the >incident) about any product. It's sad but sales reps seem to be rather >aggressive in acting to silence criticism of their (extremely expensive) >products. > >-Erin > >NOTICE TO RECIPIENT: If you are not the intended recipient of this >e-mail, you are prohibited >from sharing, copying, or otherwise using or disclosing its contents. If >you have received this e-mail in error, please notify the sender >immediately by reply e-mail and permanently delete this e-mail and any >attachments without reading, forwarding or saving them. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From mari.ann.mailhiot <@t> leica-microsystems.com Mon Oct 23 16:33:56 2006 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Mon Oct 23 16:33:48 2006 Subject: [Histonet] Cryostat decontamination policy In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC787@ORLEV03.hca.corpad.net> Message-ID: Kari I will send a copy of the disinfection protocol under separate cover. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Zajic Kari" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Cryostat decontamination policy 10/23/2006 12:38 PM Hello all, would anyone be willing to share their cryostat decontamination policy? We do not have a UV cryostat (we have a Leica 1850) and were wondering if anyone had a procedure to disinfect without defrosting? Thank you in advance... Kari Marie Zajic HTL,MLT Histology/Pathology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From victor <@t> pathology.washington.edu Mon Oct 23 16:42:40 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Oct 23 16:42:48 2006 Subject: [Histonet] Computer Orders for Pathology In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEEA6@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEEA6@sjhaexc02.sjha.org> Message-ID: <453D3750.7040305@pathology.washington.edu> Joyce, We have not gone paperless, but probably could for about 50% of our workload. We use PowerPath which has an orders interface. We currently receive messages from EPIC, but only our satellite clinics are using it. We receive the specimen and requisition which both contain the order number. By entering the order number, patient demographics, referring physician, relevant clinical information and specimen description are pulled in. We just updated the specimen information to match our panels. So we could almost go paperless with this since the order number is on the specimen label. The problem for us is that EPIC uses a MRN that is not currently compatible with our billing system. If it is a new patient, we need the paperwork and insurance information to register the patient. At this time I don't know what the plan is for the medical center (internally) to interface to us. Victor Weems, Joyce wrote: > You guys were great to share info with me from my recent workload questions. Thanks so much. > > Another one... > > How many of you are no longer using paper requisitions? We have been requested to eliminate ours and to order through our IDX system. If anyone does this, would you please share your experience? It seems to me we don't have enough spaces to include the info our pathologists need, but I may have blinders on. I'd appreciate your experience and how it has worked for you. > > We seem to have a hard time getting paper requisitions completed, not sure how going paperless could work! > > Thanks, > Joyce > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From tucsonht <@t> gmail.com Mon Oct 23 18:56:35 2006 From: tucsonht <@t> gmail.com (Jason Rogers) Date: Mon Oct 23 18:56:42 2006 Subject: [Histonet] Histology School Message-ID: <4530884d0610231656v301be26bx69a8e3252ad2b0a2@mail.gmail.com> I'm currently enrolled in a Histotechnician program at Pima Community Collage in Tucson, AZ. What sort of feedback are you looking for? I have had a wonderful experience in the program and seem to be getting a lot more of the academic background on tissue and stains that some of the experienced HT lack. Currently the program I am enrolled in requires about two years to complete and is an associate degree program. I've had about a year of gross anatomy and microanatomy; as well as more basic biology classes. Right now I am working my way through the first of two co-ops that we are required to complete. I hope this has helped, if you need more specific details please don't hesitate to email me. -J Rogers With the number of Histology Schools decreasing and the critical shortage of > qualified Histologists, we are considering establishing a training program > to provide the academic as well as the practical day to day applications > necessary for anyone interested in becoming a Histologist. I would > appreciate any feedback from anyone who has considered this option or is > presently in a Histology program. > Robert King, BS, MA > Laboratory Services Director From omnivore98 <@t> yahoo.com Mon Oct 23 19:05:03 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Mon Oct 23 19:05:11 2006 Subject: [Histonet] prefer fixative vs formalin Message-ID: <20061024000504.7829.qmail@web31305.mail.mud.yahoo.com> Hello Fellow Histonetters, I am trying to eliminate some of the formalin in our lab and my safety officer has suggested that we can have our outpatient facilities and surgery department send our specimens to us in prefer and then transfer to formalin in our processor. Any experience and or thoughts on this. Additionally, what is your experience with switching over completely to prefer?? We run a normal overnight processing, no microwave processing and we do H&E, special stains, and immunohistochemistry on the Ventana platform. Thank you in advance. --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From kimtournear <@t> yahoo.com Mon Oct 23 19:47:38 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Mon Oct 23 19:47:43 2006 Subject: [Histonet] Good Bye Message-ID: <20061024004738.80065.qmail@web37701.mail.mud.yahoo.com> I think the person/people responsible for this whole incident should be professional enough to step up to the plate, regardless of the right and wrong involved....I have to agree with the others...this is a site for histotechs NOT vendors.... Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From srwilkes <@t> gmail.com Mon Oct 23 21:52:43 2006 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Mon Oct 23 21:53:50 2006 Subject: [Histonet] seeking NYC histology position Message-ID: <8e5827cf0610231952k8c824f6s3bd7644eb8d216ab@mail.gmail.com> Hello I am seeking a histology position either in NYC or in the surrounding areas. I have a masters in biology and 2 years in a histology lab/pathology lab, as well as some histology teaching experience. I also have great experience with immunohistochemistry proceedures. Thank you in advance Steven srwilkes@gmail.com From argonautro <@t> yahoo.com Tue Oct 24 01:34:19 2006 From: argonautro <@t> yahoo.com (Pirici Daniel) Date: Tue Oct 24 01:34:24 2006 Subject: [Histonet] EDTA Ph 9 antigen retrieval question Message-ID: <20061024063419.69878.qmail@web31503.mail.mud.yahoo.com> Hi histoneters! I was looking for the recipe to make Tris EDTA pH 9 for antigen retrieval. Everybody mentions using EDTA, but judging by the molarity of the solution and the quantity to add I guessed that in fact it is not EDTA (M = 292,24), but a di-sodium, di-hydrated salt (M =372,24) also known as titriplex III. Is this correct? Does anybody know if the Na is essential for the retrieval action of thebuffer? If I do not have Titriplex III and I just use EDTA, would it be enough? Many thanks from now! Daniel Pirici, MD University of Medicine and Pharmacy, Craiova, Romania From argonautro <@t> yahoo.com Tue Oct 24 01:34:19 2006 From: argonautro <@t> yahoo.com (Pirici Daniel) Date: Tue Oct 24 01:34:26 2006 Subject: [Histonet] EDTA Ph 9 antigen retrieval question Message-ID: <20061024063419.69878.qmail@web31503.mail.mud.yahoo.com> Hi histoneters! I was looking for the recipe to make Tris EDTA pH 9 for antigen retrieval. Everybody mentions using EDTA, but judging by the molarity of the solution and the quantity to add I guessed that in fact it is not EDTA (M = 292,24), but a di-sodium, di-hydrated salt (M =372,24) also known as titriplex III. Is this correct? Does anybody know if the Na is essential for the retrieval action of thebuffer? If I do not have Titriplex III and I just use EDTA, would it be enough? Many thanks from now! Daniel Pirici, MD University of Medicine and Pharmacy, Craiova, Romania From cgfields <@t> lexhealth.org Tue Oct 24 08:12:45 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Tue Oct 24 08:13:05 2006 Subject: [Histonet] Special Password for Histotechs Message-ID: Maybe we should go to a password or something to get on the Histonet that vendors aren't privy to. For someone to almost lose their job because of something said on the Histonet ...is unbelievable. That tells me I sure wouldn't want to work for such a group of people. It's a pain to enter passwords but this is ridicules that we cannot speak freely on our own web site. We have passwords for everything in the world here at the hospital. It is like a federal offense to give it out also..... Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From kewleys <@t> health.missouri.edu Tue Oct 24 08:47:46 2006 From: kewleys <@t> health.missouri.edu (Kewley, Sharyl F.) Date: Tue Oct 24 08:48:01 2006 Subject: [Histonet] RE: Histonet Digest, Vol 35, Issue 35 References: Message-ID: <93D0418A67D27C47BD90CF3A0DCC39F524A27E@UM-XMAIL04.um.umsystem.edu> Hi Medea, We use the Gomori's method for staining our reticulum for bone marrow and have very good results. The method is found in the Histotechnology - A Self-Instructional Text, by: Freda Carson. I use the drop-on-slide method with most of the steps. The key is to mix the staining solution well. It's hard to tell you instead of show you. You might have to experiment with the stain a few times to get it right, but if you follow the instructions in the book I think it will work for you. If you should have further questions you could call me at the number listed below. Good luck and happy staining. Sharyl Kewley, HT (ASCP) Columbia Reginal Hospital 404 Keene Street Columbia, MO 65201 573-875-9354 ________________________________ ) 7. Re: RE: Using the best Retic Silver Stain for Bone marrow biopsies (Rene J Buesa) Message: 7 Date: Sat, 21 Oct 2006 05:56:07 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] RE: Using the best Retic Silver Stain for Bone marrow biopsies To: "McGraw, Medea J" , histonet@lists.utsouthwestern.edu Message-ID: <20061021125607.41159.qmail@web61222.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Medea J.: In my experience, after trying many different procedures, I found that the best and more consistent is Gomori's ammoniacal silver method. Ren? J. "McGraw, Medea J" wrote: I am searching for an optimal method for providing the best Retic silver stain, aside from using automation and kits, on bone marrow biopsies. Currently, we have been using the Wilder's technique and ran into inconsistencies in the past 5 months or so. Is there a better method than your lab is currently using that you would care to share with me to try? I have also tried the Gridley's and the results are good fibers but very muddy. We are currently using a Nexus to help us "get by" but the kits are $$$ and it's just a temporary fix. I am interested in all methods (microwave or not). I would appreciate any suggestions. _____________________________________________ From TMcNemar <@t> lmhealth.org Tue Oct 24 09:13:11 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Oct 24 09:13:22 2006 Subject: [Histonet] Formalin storage Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F415@lmhsmail.lmhealth.org> I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From rjbuesa <@t> yahoo.com Tue Oct 24 09:23:22 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:23:38 2006 Subject: [Histonet] Special Password for Histotechs In-Reply-To: Message-ID: <20061024142322.26800.qmail@web61215.mail.yahoo.com> Carole: I don't think that would be possible because we are accessing a public web-site and anybody can access it by just typing the web-site name, and no passwords are required for that. Is the same thing when you go to any website or when you are looking for something in the www. Ren? J. Carole Fields wrote: Maybe we should go to a password or something to get on the Histonet that vendors aren't privy to. For someone to almost lose their job because of something said on the Histonet ...is unbelievable. That tells me I sure wouldn't want to work for such a group of people. It's a pain to enter passwords but this is ridicules that we cannot speak freely on our own web site. We have passwords for everything in the world here at the hospital. It is like a federal offense to give it out also..... Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue Oct 24 09:30:36 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:30:51 2006 Subject: [Histonet] Good Bye In-Reply-To: Message-ID: <20061024143036.17510.qmail@web61223.mail.yahoo.com> Janice: I totally agree with you and appreciate what happened to Joe, but let me play the Devil's advocate now: IF (a big IF) Joe's place of employment has a WRITTEN policy stating, for example, that "no employee is allowed to publicly express opinions regarding to procedures used in the work place", then they had the "right" to reprimand Joe regardless if his comments were positive or negative. This is not if that policy curves the constitutional "free of expression right"; IF such a policy is in place it is an internal one and IF all employees know it and signed to obey it, then the reprimand has to be accepted (as Joe did, by the way). Just my opinion! Ren? J. Janice Mahoney wrote: Joe said this situation "almost cost him his job". As a manager I can't imagine an employer firing someone for stating an opinion about a product or methodology. (Now, if Joe were spending all his time on the net and not doing his job, that would be a different story. Those of us who keep up on this net know Joe to be a very dedicated professional so I doubt that is the case.) We are all in this together and as far as I'm concerned it is about one thing; PATIENT CARE. It does not matter if you are in research or in a clinical lab, it is all about the patients. Any time a one of us says anything negative about a product it is because we are striving for quality patient care. It is like I tell my staff; We have to leave our egos outside the lab. If your quality is not up to snuff it is not about you, it is about the patient. My point to the vendors is this; If we are not happy about your products leave your ego outside. We want products that will help us deliver quality slides. That is all that matters. Take the criticism and make your products better or more consistent. Enough, I'll get off my soap box. The above opinions do not reflect those of my employer, boss, dog or maybe even me. Jan Omaha >>> LuAnn Anderson 10/23/2006 11:15 AM >>> Joe, I would say that it is your companies loss and that the vender in question probably hasn't done themselves any good here!! Good luck to you--with your knowledge and experience, you should be able to find a better position in no time, hopefully with a company that will value your opinion. LuAnn At 02:46 PM 10/20/2006, Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not > only did I get flamed, I was burned to a crisp. I hope you are > happy. You think you may have won, but all you did was hamper a so > called open forum so people could exchange ideas and opinions. Now, > many people will be afraid to give their honest opinion for fear of > retribution, Sleep tight tonight. You almost cost me my job. Losing > my job over a vendor is just not worth it. I don't need any help, I > can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that > people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > >Joe Nocito >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From rjbuesa <@t> yahoo.com Tue Oct 24 09:34:43 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:34:56 2006 Subject: [Histonet] Re: goodbye In-Reply-To: <4.3.2.7.2.20061023125201.00cc6480@algranth.inbox.email.arizona.edu> Message-ID: <20061024143443.30792.qmail@web61215.mail.yahoo.com> Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! Ren? J. Andrea Grantham wrote: It happened to me also - maybe this happens more than we think??? I was chastised by a vendor when I asked if anybody else was experiencing a certain phenomenon with one of their products. He told me that I should have gone to them instead of asking my question on histonet. Well, I did and really didn't get any help from them when I did this before going to histonet. Instead they were trying to tell me to change my technique. I don't know about you, but after you do something one way for 25 or more years it would seem that it wouldn't be the technique - or not usually. Long story short, we danced around this problem until somebody finally told me they had a bad batch of base "product" that went into certain lots. Bingo! Thank God for the brave soul that finally admitted that the company did have a problem but why couldn't they admit it weeks sooner and save me the grief that I had with my tissues? By the way, there were others out in histoland having the same problem. Hope they got it fixed too. Andi At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: >Amos wrote: "This happened to me before too. A vendor that will remain >nameless >(that almost everyone has some equipment sold to them by these people) >took >issue with my stating that we were having trouble with an expensive item >they sold us when someone asked about this item on the Histonet." > >I am sorry to say that this happened to me as well at a previous job. >Since then I am always VERY careful about what I say (not much after the >incident) about any product. It's sad but sales reps seem to be rather >aggressive in acting to silence criticism of their (extremely expensive) >products. > >-Erin > >NOTICE TO RECIPIENT: If you are not the intended recipient of this >e-mail, you are prohibited >from sharing, copying, or otherwise using or disclosing its contents. If >you have received this e-mail in error, please notify the sender >immediately by reply e-mail and permanently delete this e-mail and any >attachments without reading, forwarding or saving them. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue Oct 24 09:37:24 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:37:35 2006 Subject: [Histonet] Good Bye In-Reply-To: <20061024004738.80065.qmail@web37701.mail.mud.yahoo.com> Message-ID: <20061024143724.76988.qmail@web61211.mail.yahoo.com> Don't hold your breath while waiting for an apology! First you will get blue and then violet untill you suffocate! Ren? J. Kim Tournear wrote: I think the person/people responsible for this whole incident should be professional enough to step up to the plate, regardless of the right and wrong involved....I have to agree with the others...this is a site for histotechs NOT vendors.... Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From Terry.Marshall <@t> rothgen.nhs.uk Tue Oct 24 09:42:30 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Oct 24 09:39:41 2006 Subject: [Histonet] A complex issue. Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C35@LIL.xRothGen.nhs.uk> By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From rjbuesa <@t> yahoo.com Tue Oct 24 09:42:42 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:42:54 2006 Subject: [Histonet] PA Programs available? In-Reply-To: <453D17E7.2030808@sch-farmville.org> Message-ID: <20061024144242.43823.qmail@web61222.mail.yahoo.com> I think it would be better for you to contact the American Association of Pathology Assistants (AAPA); they have a web-site and for sure could help you. Ren? J. Jennie Jenkins wrote: Does anyone out there know if there are any PA programs that offer online coursework so that I would only have to travel for the labs? Or is there anyone out there who has sit for the exam through the OJT route and could shed some light. I already know the requirements, I just don't know if I can pass the exam based on the fact that we do such limited variety of specimens in our hospital. Thanks in advance. Jennie Jenkins, MT Southside Community Hospital Farmville, VA 23901 434-315-2618 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From Lynne.Bell <@t> hitchcock.org Tue Oct 24 09:45:30 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Tue Oct 24 09:45:42 2006 Subject: [Histonet] Formalin storage In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F415@lmhsmail.lmhealth.org> Message-ID: We have prefilled containers of formalin for the smaller specimens in Surgery. For larger specimens, there are a gallon jugs of 10% formalin available and they are used under a hood. Our birthing center sends the fresh placenta to us and Histology personnel (or laboratory personnel after hours) adds the formalin. Lynne From rjbuesa <@t> yahoo.com Tue Oct 24 09:45:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:45:45 2006 Subject: [Histonet] A complex issue. In-Reply-To: <63984BC3A63FF542AF6EF0A237F38F4D7E4C35@LIL.xRothGen.nhs.uk> Message-ID: <20061024144538.59842.qmail@web61213.mail.yahoo.com> And what did you do about it (now that you have made it public)? Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From Terry.Marshall <@t> rothgen.nhs.uk Tue Oct 24 09:47:40 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Oct 24 09:45:48 2006 Subject: [Histonet] Good Bye Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C36@LIL.xRothGen.nhs.uk> Rene said "IF (a big IF) Joe's place of employment has a WRITTEN policy stating, for example, that "no employee is allowed to publicly express opinions regarding to procedures used in the work place", then they had the "right" to reprimand Joe regardless if his comments were positive or negative." I would interpret that as precluding an employee from criticising practices and procedures in use at their place of employment, that is to say, essentially, criticising the employer. Such would not be the case here. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 24 October 2006 15:31 To: Janice Mahoney; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; ander093@tc.umn.edu Subject: Re: [Histonet] Good Bye Janice: I totally agree with you and appreciate what happened to Joe, but let me play the Devil's advocate now: IF (a big IF) Joe's place of employment has a WRITTEN policy stating, for example, that "no employee is allowed to publicly express opinions regarding to procedures used in the work place", then they had the "right" to reprimand Joe regardless if his comments were positive or negative. This is not if that policy curves the constitutional "free of expression right"; IF such a policy is in place it is an internal one and IF all employees know it and signed to obey it, then the reprimand has to be accepted (as Joe did, by the way). Just my opinion! Ren? J. Janice Mahoney wrote: Joe said this situation "almost cost him his job". As a manager I can't imagine an employer firing someone for stating an opinion about a product or methodology. (Now, if Joe were spending all his time on the net and not doing his job, that would be a different story. Those of us who keep up on this net know Joe to be a very dedicated professional so I doubt that is the case.) We are all in this together and as far as I'm concerned it is about one thing; PATIENT CARE. It does not matter if you are in research or in a clinical lab, it is all about the patients. Any time a one of us says anything negative about a product it is because we are striving for quality patient care. It is like I tell my staff; We have to leave our egos outside the lab. If your quality is not up to snuff it is not about you, it is about the patient. My point to the vendors is this; If we are not happy about your products leave your ego outside. We want products that will help us deliver quality slides. That is all that matters. Take the criticism and make your products better or more consistent. Enough, I'll get off my soap box. The above opinions do not reflect those of my employer, boss, dog or maybe even me. Jan Omaha >>> LuAnn Anderson 10/23/2006 11:15 AM >>> Joe, I would say that it is your companies loss and that the vender in question probably hasn't done themselves any good here!! Good luck to you--with your knowledge and experience, you should be able to find a better position in no time, hopefully with a company that will value your opinion. LuAnn At 02:46 PM 10/20/2006, Joe Nocito wrote: > Just as I expected, some one called my place of employment. Not > only did I get flamed, I was burned to a crisp. I hope you are > happy. You think you may have won, but all you did was hamper a so > called open forum so people could exchange ideas and opinions. Now, > many people will be afraid to give their honest opinion for fear of > retribution, Sleep tight tonight. You almost cost me my job. Losing > my job over a vendor is just not worth it. I don't need any help, I > can do it just fine by myself. > Netters, it's been fun. I had many laughs and received a lot of > information. I pass the baton on to someone else. It's sad that > people can not give their honest opinion. > We'll be meeting again, you can be sure of that. Good bye and good luck. > > >Joe Nocito >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 24 09:50:42 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:50:50 2006 Subject: [Histonet] Computer Orders for Pathology In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEEA6@sjhaexc02.sjha.org> Message-ID: <20061024145042.24027.qmail@web61223.mail.yahoo.com> Since 1994 I went paperless and it was just fantastic. The thing depends on your purchasing department and how they implement the procedure. Some places are better than others in doing that. Ren? J. "Weems, Joyce" wrote: You guys were great to share info with me from my recent workload questions. Thanks so much. Another one... How many of you are no longer using paper requisitions? We have been requested to eliminate ours and to order through our IDX system. If anyone does this, would you please share your experience? It seems to me we don't have enough spaces to include the info our pathologists need, but I may have blinders on. I'd appreciate your experience and how it has worked for you. We seem to have a hard time getting paper requisitions completed, not sure how going paperless could work! Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From JWEEMS <@t> sjha.org Tue Oct 24 09:54:18 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Oct 24 09:54:42 2006 Subject: [Histonet] A complex issue. Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEED1@sjhaexc02.sjha.org> I received a call from someone conducting a survey on Ventana - said she was a medical writer. Just a little over a week ago... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, October 24, 2006 10:43 AM To: Rene J Buesa; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Terry.Marshall <@t> rothgen.nhs.uk Tue Oct 24 09:58:13 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Oct 24 09:55:57 2006 Subject: [Histonet] A complex issue. Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C37@LIL.xRothGen.nhs.uk> After expending much time and effort in determining that the firm was genuine, I declined. There are many things I will not do for money. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 24 October 2006 15:46 To: Marshall Terry Dr, Consultant Histopathologist; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. And what did you do about it (now that you have made it public)? Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 24 09:57:24 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 09:57:32 2006 Subject: [Histonet] prefer fixative vs formalin In-Reply-To: <20061024000504.7829.qmail@web31305.mail.mud.yahoo.com> Message-ID: <20061024145724.76738.qmail@web61212.mail.yahoo.com> Transfering tissues fixed in Prefer to formalin would completely "defeat" the purpose of eliminating a noxious chemical like formalin from the histology lab. Any tissue processor can use Prefer in the first 2 stations if you chose to do so. If in doubt run a paralel test of tissues fixed with botth fixatives and let the pathologist evaluate the results on a "blind" basis (fixative unknwon to them). Prefer has been around for many years already and is a "well known" formalin substitute. Ren? J. Heather Renko wrote: Hello Fellow Histonetters, I am trying to eliminate some of the formalin in our lab and my safety officer has suggested that we can have our outpatient facilities and surgery department send our specimens to us in prefer and then transfer to formalin in our processor. Any experience and or thoughts on this. Additionally, what is your experience with switching over completely to prefer?? We run a normal overnight processing, no microwave processing and we do H&E, special stains, and immunohistochemistry on the Ventana platform. Thank you in advance. --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From doug <@t> ppspath.com Tue Oct 24 10:12:50 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 10:10:06 2006 Subject: [Histonet] A complex issue. In-Reply-To: <63984BC3A63FF542AF6EF0A237F38F4D7E4C37@LIL.xRothGen.nhs.uk> Message-ID: I also received a call from a consulting firm wanting input on a different histology situation (not mentioning any company or issue). I gave them my two cents and told them to keep the change. My opinions are free. I can see someone in the future paying me to keep my opinions to myself. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, October 24, 2006 10:58 AM To: Rene J Buesa; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. After expending much time and effort in determining that the firm was genuine, I declined. There are many things I will not do for money. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 24 October 2006 15:46 To: Marshall Terry Dr, Consultant Histopathologist; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. And what did you do about it (now that you have made it public)? Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 24 10:12:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 10:12:47 2006 Subject: [Histonet] Formalin storage In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F415@lmhsmail.lmhealth.org> Message-ID: <20061024151239.73354.qmail@web61220.mail.yahoo.com> We received specimens directly in formalin, large and small. We also had to keep small bottles with formalin so the PA could divide the original specimen into parts for histology. The empty containers were kept in cabinets in the surgical description area but we never stocked more than those needed for one day. Larger containers were kept inside a hood in the morgue. We had a strong program for formalin monitoring to assure a safe environment. There are really not defined regulations and the actual setting is open to the "better judgment" of the inspectors. Ren? J. Tom McNemar wrote: I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From PMonfils <@t> Lifespan.org Tue Oct 24 10:14:49 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Oct 24 10:15:06 2006 Subject: [Histonet] Formalin storage Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BA0@LSRIEXCH1.lsmaster.lifespan.org> Not to mention that some surgical specimens may be infectious. Does JCAHO want unfixed infectious material being carried around the hospital? I work in a 700 bed facility. All surgical specimens are delivered fresh to the prosecting room, which is attached to the surgical suite, where they are described and dissected by residents or pathology assistants, who place the selected tissue samples into cassettes, and then directly into formalin. Nothing leaves the surgical area enroute to the lab until it is in formalin. Except of course fluids intended for cell blocks. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tom > McNemar > Sent: Tuesday, October 24, 2006 7:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin storage > > I have a meeting tomorrow to discuss the keeping of formalin in the > surgery and L&D areas. The hospital just had a JCAHO inspection and the > inspectors didn't like it very much. They suggested that the 5 gallon > carboys should be stored in hoods or removed from the areas. They went on > to say that all specimens should be taken to Histology as fresh specimens > and that the Histology personnel should be the ones putting formalin on > the specimens. The inspectors said that that was how all of the larger > hospitals did it but no one that I have spoken to does it that way. > > So my question.... Does anybody (or a significant number of you) do it > this way? > > Personally, I can't see how this can be good for the specimens. Specimens > would have to be delivered immediately and someone would have to be > available 24/7 to take care of them. I just don't see how it can work. I > would appreciate any thoughts. Thanks. > > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JWEEMS <@t> sjha.org Tue Oct 24 10:16:58 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Oct 24 10:17:17 2006 Subject: [Histonet] A complex issue. Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEED3@sjhaexc02.sjha.org> There was no money involved for mine and I was not aware that it was about Ventana until far into the interview, which took ~10 min. I did tell them that I would not be interested in any Ventana instrumentation or products. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, October 24, 2006 10:58 AM To: Rene J Buesa; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. After expending much time and effort in determining that the firm was genuine, I declined. There are many things I will not do for money. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 24 October 2006 15:46 To: Marshall Terry Dr, Consultant Histopathologist; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. And what did you do about it (now that you have made it public)? Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Lance.Erickson <@t> intermountainmail.org Tue Oct 24 10:17:52 2006 From: Lance.Erickson <@t> intermountainmail.org (Lance Erickson) Date: Tue Oct 24 10:18:23 2006 Subject: [Histonet] PA Programs available? Message-ID: <8B08EC394B366D4A895DD5686D6AFE4A57FEB1@LP-EXCHVS08.CO.IHC.COM> See the AAPA website www.pathologistsassistants.org they have all the PA programs listed. I don't think any of them offer online coursework. I sat for the exam this year throught the OJT route. Even though I see limited specimen types because of our patient population at a children's hospital I still was doing more than the minimum 20 types of complex specimens to qualify for the OJT route. This really has little to do with the content of the exam. The best thing to know for the exam is Robbins textbook "Pathologic Basis of Disease" front to back. Lance Erickson, PA, HTL Anatomic Pathology Supervisor Primary Children's Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennie Jenkins Sent: Monday, October 23, 2006 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PA Programs available? Does anyone out there know if there are any PA programs that offer online coursework so that I would only have to travel for the labs? Or is there anyone out there who has sit for the exam through the OJT route and could shed some light. I already know the requirements, I just don't know if I can pass the exam based on the fact that we do such limited variety of specimens in our hospital. Thanks in advance. Jennie Jenkins, MT Southside Community Hospital Farmville, VA 23901 434-315-2618 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 24 10:21:01 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 24 10:20:18 2006 Subject: [Histonet] Formalin storage Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC821@wahtntex2.waht.swest.nhs.uk> We received specimens directly in formalin, large and small. We also had to keep small bottles with formalin so the PA could divide the original specimen into parts for histology. The empty containers were kept in cabinets in the surgical description area but we never stocked more than those needed for one day. Larger containers were kept inside a hood in the morgue. We had a strong program for formalin monitoring to assure a safe environment. There are really not defined regulations and the actual setting is open to the "better judgment" of the inspectors. Ren? J. Risk assess the transportation of specimens in formalin, determine the risk, decide on the training needed to mitigate the risk, train and monitor. Just because something has a risk does not mean it can?t be carried out; you just need to make sure you reduce the risk as much as humanly possible, train appropriately and monitor regularly. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Nobody grows old merely by living a number of years. We grow old by deserting our ideals. Years may wrinkle the skin, but to give up enthusiasm wrinkles the soul. --Samuel Ullman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Tue Oct 24 10:16:58 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Oct 24 10:20:33 2006 Subject: [Histonet] A complex issue. Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C39@LIL.xRothGen.nhs.uk> LOL Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Douglas D Deltour [mailto:doug@ppspath.com] Sent: 24 October 2006 16:13 To: Marshall Terry Dr, Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. I also received a call from a consulting firm wanting input on a different histology situation (not mentioning any company or issue). I gave them my two cents and told them to keep the change. My opinions are free. I can see someone in the future paying me to keep my opinions to myself. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, October 24, 2006 10:58 AM To: Rene J Buesa; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. After expending much time and effort in determining that the firm was genuine, I declined. There are many things I will not do for money. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 24 October 2006 15:46 To: Marshall Terry Dr, Consultant Histopathologist; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. And what did you do about it (now that you have made it public)? Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Oct 24 10:25:59 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Oct 24 10:26:42 2006 Subject: [Histonet] A complex issue. In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEED3@sjhaexc02.sjha.org> Message-ID: In response to Doug's comments about being "paid to keep his comments to himself" (I deleted his email before I thought of this witty reply) - I think that's called "blackmail". You'll have to check with Terry about that one! "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/24/2006 10:16 AM To "Marshall Terry Dr, Consultant Histopathologist" , "Rene J Buesa" , , cc Subject RE: [Histonet] A complex issue. There was no money involved for mine and I was not aware that it was about Ventana until far into the interview, which took ~10 min. I did tell them that I would not be interested in any Ventana instrumentation or products. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, October 24, 2006 10:58 AM To: Rene J Buesa; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. After expending much time and effort in determining that the firm was genuine, I declined. There are many things I will not do for money. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 24 October 2006 15:46 To: Marshall Terry Dr, Consultant Histopathologist; LMargraf@childmed.dallas.tx.us; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A complex issue. And what did you do about it (now that you have made it public)? Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: By a quirk of fate, I received this e-mail this morning. I received an identical one about 18 months ago. Dear Dr. Marshall, I am contacting you due to your expertise in Pathology. My firm, Clinical Advisors (www.clinicaladvisors.com), is an independent research firm that focuses on the health care industry. We currently have a client who is interested in getting your opinion of the Ventana Symphony slide stainer. The client is interested in conducting a 30-45 minute phone interview with you to discuss this topic. Further, for your time we would pay you an honorarium, made payable either to you directly or to your organization. Please let me know if this opportunity interests you. If you have any questions or would like more information, feel free to contact me via email or phone at (646) 873-3005. Thank you for your time and consideration. I look forward to hearing from you soon. Sincerely, Corinne McGown ~~~~~~~~~~~~~~~~ Corinne McGown, MPH Clinical Advisors 444 Madison Avenue, 26th Floor New York, NY 10022 Telephone: 646.873.3005 www.clinicaladvisors.com Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 24 10:32:59 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 24 10:32:09 2006 Subject: [Histonet] Carter Review Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC822@wahtntex2.waht.swest.nhs.uk> Subsequent to the Carter Review I wonder what the perception of Brits is to its effects, if any. It makes heavy play of managed networks and alludes to pilot Sites; my question is simple (in keeping with my intellect). Does Carter assume that his recommendations start at Trust level or at managed network level? If it assumes networks are not inplace then the pilot Sites I assume must be looking at their birth, but if the Review assumes that they are in place then the pilot Sites will be the managed network not the Trust. What do you think? Those with networks may have a different opinion than those of us devoid of them at the moment; I know the Sites don't know either, do they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Nobody grows old merely by living a number of years. We grow old by deserting our ideals. Years may wrinkle the skin, but to give up enthusiasm wrinkles the soul. --Samuel Ullman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ree3 <@t> leicester.ac.uk Tue Oct 24 10:47:02 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Oct 24 10:47:20 2006 Subject: [Histonet] Suggested Special Password for Histotechs In-Reply-To: Message-ID: ABRACADAVERA?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: 24 October 2006 14:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Password for Histotechs Maybe we should go to a password or something to get on the Histonet that vendors aren't privy to. For someone to almost lose their job because of something said on the Histonet ...is unbelievable. That tells me I sure wouldn't want to work for such a group of people. It's a pain to enter passwords but this is ridicules that we cannot speak freely on our own web site. We have passwords for everything in the world here at the hospital. It is like a federal offense to give it out also..... Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Oct 24 10:54:59 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Oct 24 10:55:08 2006 Subject: [Histonet] Suggested Special Password for Histotechs In-Reply-To: References: Message-ID: <453E3753.7070304@pathology.washington.edu> How about Supercalifragilisticexpialidocious! Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Edwards, R.E. wrote: > ABRACADAVERA?. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole > Fields > Sent: 24 October 2006 14:13 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Special Password for Histotechs > > Maybe we should go to a password or something to get on the Histonet > that vendors aren't privy to. For someone to almost lose their job > because of something said on the Histonet ...is unbelievable. That > tells me I sure wouldn't want to work for such a group of people. It's > a pain to enter passwords but this is ridicules that we cannot speak > freely on our own web site. We have passwords for everything in the > world here at the hospital. > It is like a federal offense to give it out also..... > > Carole Fields, HT,ASCP > Pathology Supervisor > Lexington Medical Center > 2720 Sunset Blvd. > W. Columbia, SC 29169 > > > _________________________________ > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of > its attachments, please be advised that you have received this email in > error and that any use, dissemination, distribution, forwarding, > printing, or copying of this email or any attached files is strictly > prohibited. If you have received this email in error, please immediately > purge it and all attachments and notify the sender by reply email or > contact the sender at the number listed above if one is provided. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From henriks <@t> southbaytech.com Tue Oct 24 11:02:08 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 11:04:15 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <20061024143443.30792.qmail@web61215.mail.yahoo.com> References: <20061024143443.30792.qmail@web61215.mail.yahoo.com> Message-ID: <453E3900.4040407@southbaytech.com> I missed the earliest part of the thread here, but from what I see it seems that someone criticized a vendor on the Histonet and then was reprimanded by his boss for doing so after the vendor complained. Now, everyone wants to ban vendors from the Histonet. I am a vendor and I belong to many listservers. End users seem to have a very skewed view of what a vendor is and what motivates us. My main business is not histology, although we do offer some products for the industry. Yes, companies are in business to make money. No question. To say that patient care is not even on their radar screen is ridiculous. If the customer's need is to improve patient care, then that is what a vendor is striving for. Vendors make money by supplying what customers want. If you want tools for better patient care, then that is what we provide. If your needs change, then our products change. We respond to your needs. If, as you say, patient care is not even on the vendor radar screen, then that probably means that customers have not made that a high priority in what they say they need. Vendors do not make money by producing ineffective products that customers don't want. The best way to ensure that customers get what they need and vendors produce products to meet those needs is to have an open forum where these issues can be freely discussed. That requires interchange between end users and vendors. Yes, there should be limitations - vendors should not overtly market their products on the Histonet. They should respond to postings with viable solutions - even if they involve their own products. Vendors should not use this as a forum to denigrate their competition. By the same token, end users should not use this as a forum to air their grievances about a product or a manufacturer. A single negative comment about a vendor or his product could have a devastating effect on their business. Perhaps your complaints are justified, but there are better ways to go about having your concerns heard. Any credible vendor would love to hear from you if you are having a problem with one of their products. Unfortunately, most customers do not contact the company. Sometimes when they do contact a company they reach the wrong person and do not get a satisfactory response. I can understand the frustration. However, sharing your frustration in such a public forum is not right. Just as it would not be right for you to criticize one of your colleagues on the Histonet. There are always the few that think vendors are their enemies and think that they are solely motivated by greed. The reality is that vendors and end users are in this together. The people that figure that out early on are generally the most successful and the one who actually drive the development of new products, new techniques and make significant strides in their field. To ban vendors from your site would be regrettable and counterproductive. The listserver should be used for a free exchange of ideas and not as a venue for criticism. Praise in public, criticize in private. Can't we all just get along? Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Rene J Buesa wrote: >Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! > Ren? J. > >Andrea Grantham wrote: > It happened to me also - maybe this happens more than we think??? >I was chastised by a vendor when I asked if anybody else was experiencing a >certain phenomenon with one of their products. He told me that I should >have gone to them instead of asking my question on histonet. Well, I did >and really didn't get any help from them when I did this before going to >histonet. Instead they were trying to tell me to change my technique. I >don't know about you, but after you do something one way for 25 or more >years it would seem that it wouldn't be the technique - or not usually. >Long story short, we danced around this problem until somebody finally told >me they had a bad batch of base "product" that went into certain lots. >Bingo! Thank God for the brave soul that finally admitted that the company >did have a problem but why couldn't they admit it weeks sooner and save me >the grief that I had with my tissues? >By the way, there were others out in histoland having the same problem. >Hope they got it fixed too. > >Andi > > > > > >At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: > > >>Amos wrote: "This happened to me before too. A vendor that will remain >>nameless >>(that almost everyone has some equipment sold to them by these people) >>took >>issue with my stating that we were having trouble with an expensive item >>they sold us when someone asked about this item on the Histonet." >> >>I am sorry to say that this happened to me as well at a previous job. >>Since then I am always VERY careful about what I say (not much after the >>incident) about any product. It's sad but sales reps seem to be rather >>aggressive in acting to silence criticism of their (extremely expensive) >>products. >> >>-Erin >> >>NOTICE TO RECIPIENT: If you are not the intended recipient of this >>e-mail, you are prohibited >> >> >>from sharing, copying, or otherwise using or disclosing its contents. If > > >>you have received this e-mail in error, please notify the sender >>immediately by reply e-mail and permanently delete this e-mail and any >>attachments without reading, forwarding or saving them. Thank you. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: >http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From LSebree <@t> uwhealth.org Tue Oct 24 11:10:25 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 24 11:10:40 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E3900.4040407@southbaytech.com> Message-ID: Well said David. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 11:02 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective I missed the earliest part of the thread here, but from what I see it seems that someone criticized a vendor on the Histonet and then was reprimanded by his boss for doing so after the vendor complained. Now, everyone wants to ban vendors from the Histonet. I am a vendor and I belong to many listservers. End users seem to have a very skewed view of what a vendor is and what motivates us. My main business is not histology, although we do offer some products for the industry. Yes, companies are in business to make money. No question. To say that patient care is not even on their radar screen is ridiculous. If the customer's need is to improve patient care, then that is what a vendor is striving for. Vendors make money by supplying what customers want. If you want tools for better patient care, then that is what we provide. If your needs change, then our products change. We respond to your needs. If, as you say, patient care is not even on the vendor radar screen, then that probably means that customers have not made that a high priority in what they say they need. Vendors do not make money by producing ineffective products that customers don't want. The best way to ensure that customers get what they need and vendors produce products to meet those needs is to have an open forum where these issues can be freely discussed. That requires interchange between end users and vendors. Yes, there should be limitations - vendors should not overtly market their products on the Histonet. They should respond to postings with viable solutions - even if they involve their own products. Vendors should not use this as a forum to denigrate their competition. By the same token, end users should not use this as a forum to air their grievances about a product or a manufacturer. A single negative comment about a vendor or his product could have a devastating effect on their business. Perhaps your complaints are justified, but there are better ways to go about having your concerns heard. Any credible vendor would love to hear from you if you are having a problem with one of their products. Unfortunately, most customers do not contact the company. Sometimes when they do contact a company they reach the wrong person and do not get a satisfactory response. I can understand the frustration. However, sharing your frustration in such a public forum is not right. Just as it would not be right for you to criticize one of your colleagues on the Histonet. There are always the few that think vendors are their enemies and think that they are solely motivated by greed. The reality is that vendors and end users are in this together. The people that figure that out early on are generally the most successful and the one who actually drive the development of new products, new techniques and make significant strides in their field. To ban vendors from your site would be regrettable and counterproductive. The listserver should be used for a free exchange of ideas and not as a venue for criticism. Praise in public, criticize in private. Can't we all just get along? Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Rene J Buesa wrote: >Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! > Ren? J. > >Andrea Grantham wrote: > It happened to me also - maybe this happens more than we think??? >I was chastised by a vendor when I asked if anybody else was experiencing a >certain phenomenon with one of their products. He told me that I should >have gone to them instead of asking my question on histonet. Well, I did >and really didn't get any help from them when I did this before going to >histonet. Instead they were trying to tell me to change my technique. I >don't know about you, but after you do something one way for 25 or more >years it would seem that it wouldn't be the technique - or not usually. >Long story short, we danced around this problem until somebody finally told >me they had a bad batch of base "product" that went into certain lots. >Bingo! Thank God for the brave soul that finally admitted that the company >did have a problem but why couldn't they admit it weeks sooner and save me >the grief that I had with my tissues? >By the way, there were others out in histoland having the same problem. >Hope they got it fixed too. > >Andi > > > > > >At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: > > >>Amos wrote: "This happened to me before too. A vendor that will remain >>nameless >>(that almost everyone has some equipment sold to them by these people) >>took >>issue with my stating that we were having trouble with an expensive item >>they sold us when someone asked about this item on the Histonet." >> >>I am sorry to say that this happened to me as well at a previous job. >>Since then I am always VERY careful about what I say (not much after the >>incident) about any product. It's sad but sales reps seem to be rather >>aggressive in acting to silence criticism of their (extremely expensive) >>products. >> >>-Erin >> >>NOTICE TO RECIPIENT: If you are not the intended recipient of this >>e-mail, you are prohibited >> >> >>from sharing, copying, or otherwise using or disclosing its contents. If > > >>you have received this e-mail in error, please notify the sender >>immediately by reply e-mail and permanently delete this e-mail and any >>attachments without reading, forwarding or saving them. Thank you. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: >http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wsimms <@t> mcintoshclinic.com Tue Oct 24 11:11:34 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Tue Oct 24 11:11:57 2006 Subject: [Histonet] Formalin storage References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F415@lmhsmail.lmhealth.org> Message-ID: <005d01c6f787$151d5f90$530114ac@w3256domain.com> At our hospital, placentas are floated in formalin in L&D. Sometimes they don't do a very good job of it, and just "anoint" them. I think it would be a silly waste of personnel time, as well as creating the possiblity of exposing staff, patients and visitors to potentially infectious material in the event of a spill, to have every placenta delivered fresh to histology. What's more hazardous, an occasional whiff of 10% NBF, or a hepatitis C infected placenta spilled in the hallway? Wesley W. Simms ----- Original Message ----- From: "Tom McNemar" To: Sent: Tuesday, October 24, 2006 10:13 AM Subject: [Histonet] Formalin storage I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Oct 24 11:16:45 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 11:14:05 2006 Subject: [Histonet] Special Password for Histotechs In-Reply-To: Message-ID: What about the Histotechs that have converted to vedorism? Who can we trust? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Tuesday, October 24, 2006 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Password for Histotechs Maybe we should go to a password or something to get on the Histonet that vendors aren't privy to. For someone to almost lose their job because of something said on the Histonet ...is unbelievable. That tells me I sure wouldn't want to work for such a group of people. It's a pain to enter passwords but this is ridicules that we cannot speak freely on our own web site. We have passwords for everything in the world here at the hospital. It is like a federal offense to give it out also..... Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Oct 24 11:14:52 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Oct 24 11:15:23 2006 Subject: [Histonet] Formalin storage Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684B69@bruexchange.digestivespecialists.com> Tom, Where I once worked, L&D was across the hall. The personnel from L&D brought the placentas over fresh and put them in the frig. When I got to work in the I put formalin on them. Seemed to work fine. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 24, 2006 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin storage I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Tue Oct 24 11:20:39 2006 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Tue Oct 24 11:21:17 2006 Subject: [Histonet] Re: goodbye - vendor perspective Message-ID: I agree. One of the things I sometimes get frustrated with regarding the Histonet is that we sometimes drag things out a little longer than I feel is necessary. Due to the nature of the Histonet, we all get bombarded with lots of emails and it'd be nice to really focus on the professional exchange of different perspectives within our technical and administrative areas. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, October 24, 2006 11:10 AM To: David Henriks Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: goodbye - vendor perspective Well said David. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 11:02 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective I missed the earliest part of the thread here, but from what I see it seems that someone criticized a vendor on the Histonet and then was reprimanded by his boss for doing so after the vendor complained. Now, everyone wants to ban vendors from the Histonet. I am a vendor and I belong to many listservers. End users seem to have a very skewed view of what a vendor is and what motivates us. My main business is not histology, although we do offer some products for the industry. Yes, companies are in business to make money. No question. To say that patient care is not even on their radar screen is ridiculous. If the customer's need is to improve patient care, then that is what a vendor is striving for. Vendors make money by supplying what customers want. If you want tools for better patient care, then that is what we provide. If your needs change, then our products change. We respond to your needs. If, as you say, patient care is not even on the vendor radar screen, then that probably means that customers have not made that a high priority in what they say they need. Vendors do not make money by producing ineffective products that customers don't want. The best way to ensure that customers get what they need and vendors produce products to meet those needs is to have an open forum where these issues can be freely discussed. That requires interchange between end users and vendors. Yes, there should be limitations - vendors should not overtly market their products on the Histonet. They should respond to postings with viable solutions - even if they involve their own products. Vendors should not use this as a forum to denigrate their competition. By the same token, end users should not use this as a forum to air their grievances about a product or a manufacturer. A single negative comment about a vendor or his product could have a devastating effect on their business. Perhaps your complaints are justified, but there are better ways to go about having your concerns heard. Any credible vendor would love to hear from you if you are having a problem with one of their products. Unfortunately, most customers do not contact the company. Sometimes when they do contact a company they reach the wrong person and do not get a satisfactory response. I can understand the frustration. However, sharing your frustration in such a public forum is not right. Just as it would not be right for you to criticize one of your colleagues on the Histonet. There are always the few that think vendors are their enemies and think that they are solely motivated by greed. The reality is that vendors and end users are in this together. The people that figure that out early on are generally the most successful and the one who actually drive the development of new products, new techniques and make significant strides in their field. To ban vendors from your site would be regrettable and counterproductive. The listserver should be used for a free exchange of ideas and not as a venue for criticism. Praise in public, criticize in private. Can't we all just get along? Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Rene J Buesa wrote: >Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! > Ren? J. > >Andrea Grantham wrote: > It happened to me also - maybe this happens more than we think??? >I was chastised by a vendor when I asked if anybody else was experiencing a >certain phenomenon with one of their products. He told me that I should >have gone to them instead of asking my question on histonet. Well, I did >and really didn't get any help from them when I did this before going to >histonet. Instead they were trying to tell me to change my technique. I >don't know about you, but after you do something one way for 25 or more >years it would seem that it wouldn't be the technique - or not usually. >Long story short, we danced around this problem until somebody finally told >me they had a bad batch of base "product" that went into certain lots. >Bingo! Thank God for the brave soul that finally admitted that the company >did have a problem but why couldn't they admit it weeks sooner and save me >the grief that I had with my tissues? >By the way, there were others out in histoland having the same problem. >Hope they got it fixed too. > >Andi > > > > > >At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: > > >>Amos wrote: "This happened to me before too. A vendor that will remain >>nameless >>(that almost everyone has some equipment sold to them by these people) >>took >>issue with my stating that we were having trouble with an expensive item >>they sold us when someone asked about this item on the Histonet." >> >>I am sorry to say that this happened to me as well at a previous job. >>Since then I am always VERY careful about what I say (not much after the >>incident) about any product. It's sad but sales reps seem to be rather >>aggressive in acting to silence criticism of their (extremely expensive) >>products. >> >>-Erin >> >>NOTICE TO RECIPIENT: If you are not the intended recipient of this >>e-mail, you are prohibited >> >> >>from sharing, copying, or otherwise using or disclosing its contents. If > > >>you have received this e-mail in error, please notify the sender >>immediately by reply e-mail and permanently delete this e-mail and any >>attachments without reading, forwarding or saving them. Thank you. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: >http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From froyer <@t> bitstream.net Tue Oct 24 11:23:52 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Oct 24 11:24:20 2006 Subject: Vendor Reply RE: [Histonet] Re: goodbye In-Reply-To: <20061024143443.30792.qmail@web61215.mail.yahoo.com> Message-ID: <005101c6f788$cd8a6590$7701a80a@Ford> I modified the Subject line so that all would know that a Vendor is joining this thread. You may wish to hit the Delete button now if you are so inclined. I am sure that I am not alone in suggesting that everyone pause and take a deep breath. Emotions are running a little high on this topic and, perhaps, getting a little one-sided. I have been a subscriber to this List for close to ten years now... and not just by "lurking" out there, but actually participating from time to time. I would be very disappointed if I were banned from this forum simply because I was a vendor. In the years that I have been on this List, I can remember dozens of times where a Histonetter was in a jam and needed technical assistance with a product or a service and it was a vendor who came to their aid, in a professional noncommercial way, with a solution to their problem. Taking it further, if you think long enough about it, I believe that all of you Histonetters can come up with examples of where a vendor or their field rep has assisted you in a way that was "above and beyond the call of duty" or that was done for no financial gain... in person or through the Histonet. I know this because you have told me so privately (for something that I did for you) or have spoken to me, again privately, in glowing terms about one or more other vendors who have served you in a time of desperate need. So let's keep everything in perspective. It would be interesting if, once in a while, these stories were shared on the Histonet. I also must dispute the notion that patient care is not on (histology) vendors "radar screens". Prior to going into business on my own, I worked for almost 20 years for a Fortune 500 global company that manufactured histology products. I can assure you, from first-hand experience that these companies would not be in business today if they did not develop products that could and would improve patient care. In addition, there is an equal focus towards the frontline lab professional that actually use the products that they develop to make the product better and more user friendly for the person that has to deal with it every work day. The development and improvement of products comes directly from the end user according to their needs and the needs of their patients. Contrary to want you might believe this dual focus is very much at the forefront of these companies R&D directors. As to being in the business of making money, yes that is the goal. Be honest here. The goal of all enterprises in a capitalistic society is to make a profit. But that goal will never be reached if your product or service does not cater to the person who will be using it and/or it does not provide results that promote the betterment of patient care. I will not apologize for this and neither should you. Understand also that just because your facility holds a non-profit status, it does not mean that it does not make a profit. It simply means that at the end of the day, they show a zero-biased balance sheet. (e.g. If they were not making a profit, how would they be able to pay your salary?). It is unfortunate that allegedly one company's actions may have tarnished all vendors reputations on this List, but I urge you not to fall into that trap. I appreciate all of you and consider you my friends and my customers. For my part, I will continue to feel this way whether I am banned or not. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, October 24, 2006 9:35 AM To: Andrea Grantham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! Ren? J. Andrea Grantham wrote: It happened to me also - maybe this happens more than we think??? I was chastised by a vendor when I asked if anybody else was experiencing a certain phenomenon with one of their products. He told me that I should have gone to them instead of asking my question on histonet. Well, I did and really didn't get any help from them when I did this before going to histonet. Instead they were trying to tell me to change my technique. I don't know about you, but after you do something one way for 25 or more years it would seem that it wouldn't be the technique - or not usually. Long story short, we danced around this problem until somebody finally told me they had a bad batch of base "product" that went into certain lots. Bingo! Thank God for the brave soul that finally admitted that the company did have a problem but why couldn't they admit it weeks sooner and save me the grief that I had with my tissues? By the way, there were others out in histoland having the same problem. Hope they got it fixed too. Andi At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: >Amos wrote: "This happened to me before too. A vendor that will remain >nameless >(that almost everyone has some equipment sold to them by these people) >took >issue with my stating that we were having trouble with an expensive item >they sold us when someone asked about this item on the Histonet." > >I am sorry to say that this happened to me as well at a previous job. >Since then I am always VERY careful about what I say (not much after the >incident) about any product. It's sad but sales reps seem to be rather >aggressive in acting to silence criticism of their (extremely expensive) >products. > >-Erin > >NOTICE TO RECIPIENT: If you are not the intended recipient of this >e-mail, you are prohibited >from sharing, copying, or otherwise using or disclosing its contents. If >you have received this e-mail in error, please notify the sender >immediately by reply e-mail and permanently delete this e-mail and any >attachments without reading, forwarding or saving them. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Oct 24 11:26:13 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Oct 24 11:26:29 2006 Subject: [Histonet] Formalin storage In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684B69@bruexchange.digestivespecialists.com> Message-ID: <007701c6f789$1fef6240$6601a8c0@Patsy> I believe we provided surgery with prefilled containers of formalin so all they had to do was open the container and put the tissue in, no pouring of chemicals or much exposure. The containers were sealed and delivered to pathology whenever. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, October 24, 2006 10:15 AM To: Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin storage Tom, Where I once worked, L&D was across the hall. The personnel from L&D brought the placentas over fresh and put them in the frig. When I got to work in the I put formalin on them. Seemed to work fine. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 24, 2006 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin storage I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDittus787 <@t> aol.com Tue Oct 24 11:30:05 2006 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Tue Oct 24 11:30:43 2006 Subject: [Histonet] Suggested Special Password for Histotechs Message-ID: <393.2ddda019.326f998d@aol.com> how about abra cadaver !!! Dana From doug <@t> ppspath.com Tue Oct 24 11:38:59 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 11:36:15 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E3900.4040407@southbaytech.com> Message-ID: Jane asks Histonet about product "A" John has used product "A" and has had many documented problems. John responds to Histonet and Jane about product "A". Product "A's" people calls John's boss because they did not like John's comments which are documented and true. So John should not answer Jane's question? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 12:02 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective I missed the earliest part of the thread here, but from what I see it seems that someone criticized a vendor on the Histonet and then was reprimanded by his boss for doing so after the vendor complained. Now, everyone wants to ban vendors from the Histonet. I am a vendor and I belong to many listservers. End users seem to have a very skewed view of what a vendor is and what motivates us. My main business is not histology, although we do offer some products for the industry. Yes, companies are in business to make money. No question. To say that patient care is not even on their radar screen is ridiculous. If the customer's need is to improve patient care, then that is what a vendor is striving for. Vendors make money by supplying what customers want. If you want tools for better patient care, then that is what we provide. If your needs change, then our products change. We respond to your needs. If, as you say, patient care is not even on the vendor radar screen, then that probably means that customers have not made that a high priority in what they say they need. Vendors do not make money by producing ineffective products that customers don't want. The best way to ensure that customers get what they need and vendors produce products to meet those needs is to have an open forum where these issues can be freely discussed. That requires interchange between end users and vendors. Yes, there should be limitations - vendors should not overtly market their products on the Histonet. They should respond to postings with viable solutions - even if they involve their own products. Vendors should not use this as a forum to denigrate their competition. By the same token, end users should not use this as a forum to air their grievances about a product or a manufacturer. A single negative comment about a vendor or his product could have a devastating effect on their business. Perhaps your complaints are justified, but there are better ways to go about having your concerns heard. Any credible vendor would love to hear from you if you are having a problem with one of their products. Unfortunately, most customers do not contact the company. Sometimes when they do contact a company they reach the wrong person and do not get a satisfactory response. I can understand the frustration. However, sharing your frustration in such a public forum is not right. Just as it would not be right for you to criticize one of your colleagues on the Histonet. There are always the few that think vendors are their enemies and think that they are solely motivated by greed. The reality is that vendors and end users are in this together. The people that figure that out early on are generally the most successful and the one who actually drive the development of new products, new techniques and make significant strides in their field. To ban vendors from your site would be regrettable and counterproductive. The listserver should be used for a free exchange of ideas and not as a venue for criticism. Praise in public, criticize in private. Can't we all just get along? Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Rene J Buesa wrote: >Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! > Ren? J. > >Andrea Grantham wrote: > It happened to me also - maybe this happens more than we think??? >I was chastised by a vendor when I asked if anybody else was experiencing a >certain phenomenon with one of their products. He told me that I should >have gone to them instead of asking my question on histonet. Well, I did >and really didn't get any help from them when I did this before going to >histonet. Instead they were trying to tell me to change my technique. I >don't know about you, but after you do something one way for 25 or more >years it would seem that it wouldn't be the technique - or not usually. >Long story short, we danced around this problem until somebody finally told >me they had a bad batch of base "product" that went into certain lots. >Bingo! Thank God for the brave soul that finally admitted that the company >did have a problem but why couldn't they admit it weeks sooner and save me >the grief that I had with my tissues? >By the way, there were others out in histoland having the same problem. >Hope they got it fixed too. > >Andi > > > > > >At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: > > >>Amos wrote: "This happened to me before too. A vendor that will remain >>nameless >>(that almost everyone has some equipment sold to them by these people) >>took >>issue with my stating that we were having trouble with an expensive item >>they sold us when someone asked about this item on the Histonet." >> >>I am sorry to say that this happened to me as well at a previous job. >>Since then I am always VERY careful about what I say (not much after the >>incident) about any product. It's sad but sales reps seem to be rather >>aggressive in acting to silence criticism of their (extremely expensive) >>products. >> >>-Erin >> >>NOTICE TO RECIPIENT: If you are not the intended recipient of this >>e-mail, you are prohibited >> >> >>from sharing, copying, or otherwise using or disclosing its contents. If > > >>you have received this e-mail in error, please notify the sender >>immediately by reply e-mail and permanently delete this e-mail and any >>attachments without reading, forwarding or saving them. Thank you. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: >http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Oct 24 11:38:10 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Oct 24 11:38:51 2006 Subject: Vendor Reply RE: [Histonet] Re: goodbye In-Reply-To: <005101c6f788$cd8a6590$7701a80a@Ford> Message-ID: <007e01c6f78a$cb40d8d0$6601a8c0@Patsy> Banning vendors from this or any other list does not prevent someone from forwarding a message about that vendor to them. Remember anything put out on the INTERNET not just HISTONET is potentially for public view, it is just the nature of the medium. Never put down in writing something that you would not want certain people to hear about, I learned that the hard way once, when I was suspended from my job for writing and distributing my opinion about another person working there. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Tuesday, October 24, 2006 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: Vendor Reply RE: [Histonet] Re: goodbye I modified the Subject line so that all would know that a Vendor is joining this thread. You may wish to hit the Delete button now if you are so inclined. I am sure that I am not alone in suggesting that everyone pause and take a deep breath. Emotions are running a little high on this topic and, perhaps, getting a little one-sided. I have been a subscriber to this List for close to ten years now... and not just by "lurking" out there, but actually participating from time to time. I would be very disappointed if I were banned from this forum simply because I was a vendor. In the years that I have been on this List, I can remember dozens of times where a Histonetter was in a jam and needed technical assistance with a product or a service and it was a vendor who came to their aid, in a professional noncommercial way, with a solution to their problem. Taking it further, if you think long enough about it, I believe that all of you Histonetters can come up with examples of where a vendor or their field rep has assisted you in a way that was "above and beyond the call of duty" or that was done for no financial gain... in person or through the Histonet. I know this because you have told me so privately (for something that I did for you) or have spoken to me, again privately, in glowing terms about one or more other vendors who have served you in a time of desperate need. So let's keep everything in perspective. It would be interesting if, once in a while, these stories were shared on the Histonet. I also must dispute the notion that patient care is not on (histology) vendors "radar screens". Prior to going into business on my own, I worked for almost 20 years for a Fortune 500 global company that manufactured histology products. I can assure you, from first-hand experience that these companies would not be in business today if they did not develop products that could and would improve patient care. In addition, there is an equal focus towards the frontline lab professional that actually use the products that they develop to make the product better and more user friendly for the person that has to deal with it every work day. The development and improvement of products comes directly from the end user according to their needs and the needs of their patients. Contrary to want you might believe this dual focus is very much at the forefront of these companies R&D directors. As to being in the business of making money, yes that is the goal. Be honest here. The goal of all enterprises in a capitalistic society is to make a profit. But that goal will never be reached if your product or service does not cater to the person who will be using it and/or it does not provide results that promote the betterment of patient care. I will not apologize for this and neither should you. Understand also that just because your facility holds a non-profit status, it does not mean that it does not make a profit. It simply means that at the end of the day, they show a zero-biased balance sheet. (e.g. If they were not making a profit, how would they be able to pay your salary?). It is unfortunate that allegedly one company's actions may have tarnished all vendors reputations on this List, but I urge you not to fall into that trap. I appreciate all of you and consider you my friends and my customers. For my part, I will continue to feel this way whether I am banned or not. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, October 24, 2006 9:35 AM To: Andrea Grantham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! Ren? J. Andrea Grantham wrote: It happened to me also - maybe this happens more than we think??? I was chastised by a vendor when I asked if anybody else was experiencing a certain phenomenon with one of their products. He told me that I should have gone to them instead of asking my question on histonet. Well, I did and really didn't get any help from them when I did this before going to histonet. Instead they were trying to tell me to change my technique. I don't know about you, but after you do something one way for 25 or more years it would seem that it wouldn't be the technique - or not usually. Long story short, we danced around this problem until somebody finally told me they had a bad batch of base "product" that went into certain lots. Bingo! Thank God for the brave soul that finally admitted that the company did have a problem but why couldn't they admit it weeks sooner and save me the grief that I had with my tissues? By the way, there were others out in histoland having the same problem. Hope they got it fixed too. Andi At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: >Amos wrote: "This happened to me before too. A vendor that will remain >nameless >(that almost everyone has some equipment sold to them by these people) >took >issue with my stating that we were having trouble with an expensive item >they sold us when someone asked about this item on the Histonet." > >I am sorry to say that this happened to me as well at a previous job. >Since then I am always VERY careful about what I say (not much after the >incident) about any product. It's sad but sales reps seem to be rather >aggressive in acting to silence criticism of their (extremely expensive) >products. > >-Erin > >NOTICE TO RECIPIENT: If you are not the intended recipient of this >e-mail, you are prohibited >from sharing, copying, or otherwise using or disclosing its contents. If >you have received this e-mail in error, please notify the sender >immediately by reply e-mail and permanently delete this e-mail and any >attachments without reading, forwarding or saving them. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Oct 24 11:40:37 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Oct 24 11:41:08 2006 Subject: [Histonet] Formalin storage In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684B69@bruexchange.digestivespecialists.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6E3F@EMAIL.archildrens.org> We do not receive our specimens fresh unless there is a reason for the specimen to be fresh, i.e. special studies, cultures. Our specimens come from the OR in formalin and from our clinics too. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, October 24, 2006 11:15 AM To: Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin storage Tom, Where I once worked, L&D was across the hall. The personnel from L&D brought the placentas over fresh and put them in the frig. When I got to work in the I put formalin on them. Seemed to work fine. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 24, 2006 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin storage I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From claudiamel2000 <@t> yahoo.com Tue Oct 24 11:53:44 2006 From: claudiamel2000 <@t> yahoo.com (claudia melidona) Date: Tue Oct 24 11:53:58 2006 Subject: [Histonet] Re:Lookin at new stainers Message-ID: <20061024165344.33805.qmail@web60812.mail.yahoo.com> Hi everyone- We are in the market for a new stainer/coverslipper. Can anyone give me some feedback on the Tissue-Tek Prisma stainer from Sakura and also any other stainer/coverslipper combo instrument . Lately there has been tooo much response from vendors so I would only like to hear from histo-techs. Claudia --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From ASenn <@t> mercy.pmhs.org Tue Oct 24 12:02:43 2006 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Tue Oct 24 12:02:59 2006 Subject: [Histonet] Formalin/Fixative Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A60@pmhs2kxch03> At our hospital, placentas are floated in formalin in L&D. Sometimes they don't do a very good job of it, and just "anoint" them. I think it would be a silly waste of personnel time, as well as creating the possiblity of exposing staff, patients and visitors to potentially infectious material in the event of a spill, to have every placenta delivered fresh to histology. What's more hazardous, an occasional whiff of 10% NBF, or a hepatitis C infected placenta spilled in the hallway? Wesley W. Simms ------------------------------------------ I work in the surgical department of a large hospital. We get our specimens in a variety of ways. Someone from the pathology department does 'pick ups' every hour to specified refrigerators in the hospital that hold fresh specimens. Sometimes the specimen is in formalin (if it's small enough to fit in a 4-6 oz. container). Sometimes larger specimens in clear biohazard bags. If a specimen is collected from a patient 'after hours' or on the weekend when there's no path tech available, a nurse brings the specimen (sometimes in formalin, sometimes not) to a large fridge in the path dept where it waits for me until the next morning. For placentas, the specimen is placed in a red biohazard bag with the patients information (label). Then placed in yet another red bio bag with yet another label. Then it's placed into a brown sack which is labeled. And THEN it's placed into a yellow bin that's delivered to the surgical pathology department, where it waits in the fridge for me to put it in formalin. Hospital policy is that nothing, nothing, nothing must be carried out of the operating room unless it's in some type of sealed specimen container (if it's small enough) and in a sealed biohazard bag. Our operating rooms do have formalin, but it's in individual containers with less than 20ml. If they need more at anytime for any specific procedure, they call the path department and we instruct them from there. Usually, we tell them to just bring the specimen fresh so the pathologist can gross it that minute. Hope my ramblings gave you some insight.... Have a great day... Amy From TJasper <@t> smdc.org Tue Oct 24 12:06:10 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Tue Oct 24 12:06:29 2006 Subject: [Histonet] Good Bye In-Reply-To: <20061024004738.80065.qmail@web37701.mail.mud.yahoo.com> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A441@SCREECH.ntcampus.smdc.org> Dear Everyone, I wouldn't hold my breath waiting for anyone to step up and say, "I did such and such and talked to so and so to shut Joe Nocito up!" Keep in mind that we really don't know what happened (unless I've missed something), albeit it seems most folks on the Histonet have a pretty good notion. If what really happened, happened, as most are speculating...I'm certain it's not in the best interest of the individual, individuals or the organization responsible to "fess up" on this public forum. That of course would be "their" view. For all we know they may have received "lawyerly" advice to keep quiet. Secondly, I don't believe that "legally" vendors could be kept off the Histonet. Despite what some might think, all of them are not evil. I'd like to believe the overwhelming majority to be ethical. As someone pointed out in an earlier post many have done a lot of good for our field. A movement to eliminate vendors from this forum would basically be the same as what alledgedly happened to Joe Nocito. Censoring free speech is never a good idea. Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kim Tournear Sent: Monday, October 23, 2006 7:48 PM To: Histonet Subject: [Histonet] Good Bye I think the person/people responsible for this whole incident should be professional enough to step up to the plate, regardless of the right and wrong involved....I have to agree with the others...this is a site for histotechs NOT vendors.... Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From mward <@t> wfubmc.edu Tue Oct 24 12:09:14 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Oct 24 12:09:32 2006 Subject: [Histonet] Gomori Trichrome green Message-ID: <61135F0455D33347B5AAE209B903A30417034C01@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this question for a colleague. She is currently using the Gomori Trichrome green on the Dako special stainer for her renal biopsies and wanted to know if anyone else is using this. If not the green, are people using the blue from Dako, and if so, which one? Thanks in advance for your information. Martha Ward Wake Forest University Baptist Medical Center From henriks <@t> southbaytech.com Tue Oct 24 12:09:02 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 12:11:14 2006 Subject: [Histonet] Re: goodbye - vendor perspective Message-ID: <453E48AE.2050505@southbaytech.com> John should answer Jane's question. John should answer Jane's question directly to Jane. John should not post his comments to the listserver as a whole. -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Douglas D Deltour wrote: >Jane asks Histonet about product "A" > >John has used product "A" and has had many documented problems. > >John responds to Histonet and Jane about product "A". > >Product "A's" people calls John's boss because they did not like John's >comments which are documented and true. > >So John should not answer Jane's question? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 12:02 PM >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >I missed the earliest part of the thread here, but from what I see it >seems that someone criticized a vendor on the Histonet and then was >reprimanded by his boss for doing so after the vendor complained. Now, >everyone wants to ban vendors from the Histonet. I am a vendor and I >belong to many listservers. End users seem to have a very skewed view >of what a vendor is and what motivates us. My main business is not >histology, although we do offer some products for the industry. Yes, >companies are in business to make money. No question. To say that >patient care is not even on their radar screen is ridiculous. If the >customer's need is to improve patient care, then that is what a vendor >is striving for. Vendors make money by supplying what customers want. >If you want tools for better patient care, then that is what we >provide. If your needs change, then our products change. We respond to >your needs. If, as you say, patient care is not even on the vendor >radar screen, then that probably means that customers have not made that >a high priority in what they say they need. Vendors do not make money >by producing ineffective products that customers don't want. The best >way to ensure that customers get what they need and vendors produce >products to meet those needs is to have an open forum where these issues >can be freely discussed. That requires interchange between end users >and vendors. > >Yes, there should be limitations - vendors should not overtly market >their products on the Histonet. They should respond to postings with >viable solutions - even if they involve their own products. Vendors >should not use this as a forum to denigrate their competition. By the >same token, end users should not use this as a forum to air their >grievances about a product or a manufacturer. A single negative comment >about a vendor or his product could have a devastating effect on their >business. Perhaps your complaints are justified, but there are better >ways to go about having your concerns heard. Any credible vendor would >love to hear from you if you are having a problem with one of their >products. Unfortunately, most customers do not contact the company. >Sometimes when they do contact a company they reach the wrong person and >do not get a satisfactory response. I can understand the frustration. >However, sharing your frustration in such a public forum is not right. >Just as it would not be right for you to criticize one of your >colleagues on the Histonet. > >There are always the few that think vendors are their enemies and think >that they are solely motivated by greed. The reality is that vendors >and end users are in this together. The people that figure that out >early on are generally the most successful and the one who actually >drive the development of new products, new techniques and make >significant strides in their field. To ban vendors from your site would >be regrettable and counterproductive. > >The listserver should be used for a free exchange of ideas and not as a >venue for criticism. Praise in public, criticize in private. > >Can't we all just get along? > >Best regards- > >David > > > From algranth <@t> u.arizona.edu Tue Oct 24 12:16:21 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Oct 24 12:16:37 2006 Subject: [Histonet] Re:Lookin at new stainers In-Reply-To: <20061024165344.33805.qmail@web60812.mail.yahoo.com> Message-ID: <4.3.2.7.2.20061024101058.00cc3658@algranth.inbox.email.arizona.edu> At 09:53 AM 10/24/2006 -0700, claudia melidona wrote: >Hi everyone- We are in the market for a new stainer/coverslipper. Can >anyone give me some feedback on the Tissue-Tek Prisma stainer from Sakura >and also any other stainer/coverslipper combo instrument . Lately there >has been tooo much response from vendors so I would only like to hear from >histo-techs. Claudia > >--------------------------------- >How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Just want to clarify this... David Henriks President South Bay Technology, Inc. said in his post to histonet this morning that: The listserver should be used for a free exchange of ideas and not as a venue for criticism. Praise in public, criticize in private. So in order to answer the above question do we answer in private in case what we have to say is not glowing feedback? What happened to a free exchange of ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Dorothy.L.Webb <@t> HealthPartners.Com Tue Oct 24 12:17:36 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Oct 24 12:17:50 2006 Subject: [Histonet] placenta Message-ID: <0E394B648E5284478A6CCB78E5AFDA27036406FB@hpes1.HealthPartners.int> We have L&D deliver the specimens to histology in a 163 oz covered container and add formalin in the histology lab. If it is on evenings and nights, they add the formalin from a 5 gallon Carboy's and we always have gloves and goggles for their use. In the daytime, we add the formalin. In our hospital, it is due to the fact that the hospital does not want formalin stored in lots of different departments where they would have to do formalin monitoring, etc. according to JACHO or CAP standards. It works out fine and also prevents possible spilling of the formalin in such a large bucket that gets heavy when all of the fluid is added! Also, good job on your addition to all of the Emails on vendors, Ford! I agree, but, also hate it when a vendor gets "heavy handed" and feels they control things in your lab. These vendors are few and far between, thank God, but, because they are run by humans, we all have come across the type. If they could all be like you, Ford, it would be a great working relationship!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From PMonfils <@t> Lifespan.org Tue Oct 24 12:21:09 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Oct 24 12:21:22 2006 Subject: [Histonet] Re: goodbye - vendor perspective Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BA1@LSRIEXCH1.lsmaster.lifespan.org> David H., While I agree with much of what you said, I nevertheless fully support the practice of seeking feedback/recommendations - positive or negative - about an expensive piece of equipment before purchasing it. Obviously, other users of the equipment are the only reliable source from which such information can be obtained. Offering a response to such a query does not constitute "airing grievances". It constitutes sharing of valuable information which consumers have a right to know. I realize that any company, even the best, can run into a problem now and then. But I don't see why that should present a problem. If I request information from users about a particular product or company, and one person responds negatively - which he/she has every right to do - while a dozen others report satisfaction, then of course I will go ahead and try the product or company myself. But if a majority of people report problems with a product or company, then of course I'll keep my distance. Companies which consistently provide good products and good service only stand to gain from such online recommendations. Companies which provide poor products and/or poor service have made their own beds and will just have to lie in them. Paul M. From gu.lang <@t> gmx.at Tue Oct 24 12:27:03 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 24 12:26:42 2006 Subject: AW: [Histonet] Re:Lookin at new stainers In-Reply-To: <20061024165344.33805.qmail@web60812.mail.yahoo.com> Message-ID: <000401c6f791$a012c0e0$c812a8c0@dielangs.at> We have lately purchased a Leica stainer combined with their glass-coverslipper. For our amount of slides and workflow it fits very well. We do about 200 HEs every day, staining takes place from 7 to 11. At the beginning there was some trouble because of a software-error, but now it works without complains. We like the easy way of "feeding" the thing with the coloured chips and the easy-to-handle touch screen. The logic of its software isn't always really apparent. So sometimes I stand before the instrument guessing what basket it will choose next. But in the end every basket is stained and coverslipped. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von claudia melidona Gesendet: Dienstag, 24. Oktober 2006 18:54 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re:Lookin at new stainers Hi everyone- We are in the market for a new stainer/coverslipper. Can anyone give me some feedback on the Tissue-Tek Prisma stainer from Sakura and also any other stainer/coverslipper combo instrument . Lately there has been tooo much response from vendors so I would only like to hear from histo-techs. Claudia --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Oct 24 12:31:28 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 12:28:43 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E48AE.2050505@southbaytech.com> Message-ID: David, If someone asked about your product (whatever that may be) and I loved it so so much, would you complain if I posted it on the listserver? I really don't think that you would mind it. So you would like for us to censor histonet of truthful opinions of products if they are not positive? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: David Henriks [mailto:henriks@southbaytech.com] Sent: Tuesday, October 24, 2006 1:09 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective John should answer Jane's question. John should answer Jane's question directly to Jane. John should not post his comments to the listserver as a whole. -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Douglas D Deltour wrote: >Jane asks Histonet about product "A" > >John has used product "A" and has had many documented problems. > >John responds to Histonet and Jane about product "A". > >Product "A's" people calls John's boss because they did not like John's >comments which are documented and true. > >So John should not answer Jane's question? > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 12:02 PM >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >I missed the earliest part of the thread here, but from what I see it >seems that someone criticized a vendor on the Histonet and then was >reprimanded by his boss for doing so after the vendor complained. Now, >everyone wants to ban vendors from the Histonet. I am a vendor and I >belong to many listservers. End users seem to have a very skewed view >of what a vendor is and what motivates us. My main business is not >histology, although we do offer some products for the industry. Yes, >companies are in business to make money. No question. To say that >patient care is not even on their radar screen is ridiculous. If the >customer's need is to improve patient care, then that is what a vendor >is striving for. Vendors make money by supplying what customers want. >If you want tools for better patient care, then that is what we >provide. If your needs change, then our products change. We respond to >your needs. If, as you say, patient care is not even on the vendor >radar screen, then that probably means that customers have not made that >a high priority in what they say they need. Vendors do not make money >by producing ineffective products that customers don't want. The best >way to ensure that customers get what they need and vendors produce >products to meet those needs is to have an open forum where these issues >can be freely discussed. That requires interchange between end users >and vendors. > >Yes, there should be limitations - vendors should not overtly market >their products on the Histonet. They should respond to postings with >viable solutions - even if they involve their own products. Vendors >should not use this as a forum to denigrate their competition. By the >same token, end users should not use this as a forum to air their >grievances about a product or a manufacturer. A single negative comment >about a vendor or his product could have a devastating effect on their >business. Perhaps your complaints are justified, but there are better >ways to go about having your concerns heard. Any credible vendor would >love to hear from you if you are having a problem with one of their >products. Unfortunately, most customers do not contact the company. >Sometimes when they do contact a company they reach the wrong person and >do not get a satisfactory response. I can understand the frustration. >However, sharing your frustration in such a public forum is not right. >Just as it would not be right for you to criticize one of your >colleagues on the Histonet. > >There are always the few that think vendors are their enemies and think >that they are solely motivated by greed. The reality is that vendors >and end users are in this together. The people that figure that out >early on are generally the most successful and the one who actually >drive the development of new products, new techniques and make >significant strides in their field. To ban vendors from your site would >be regrettable and counterproductive. > >The listserver should be used for a free exchange of ideas and not as a >venue for criticism. Praise in public, criticize in private. > >Can't we all just get along? > >Best regards- > >David > > > From doug <@t> ppspath.com Tue Oct 24 12:40:09 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 12:37:17 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BA1@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: Hear Hear!! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Tuesday, October 24, 2006 1:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Re: goodbye - vendor perspective David H., While I agree with much of what you said, I nevertheless fully support the practice of seeking feedback/recommendations - positive or negative - about an expensive piece of equipment before purchasing it. Obviously, other users of the equipment are the only reliable source from which such information can be obtained. Offering a response to such a query does not constitute "airing grievances". It constitutes sharing of valuable information which consumers have a right to know. I realize that any company, even the best, can run into a problem now and then. But I don't see why that should present a problem. If I request information from users about a particular product or company, and one person responds negatively - which he/she has every right to do - while a dozen others report satisfaction, then of course I will go ahead and try the product or company myself. But if a majority of people report problems with a product or company, then of course I'll keep my distance. Companies which consistently provide good products and good service only stand to gain from such online recommendations. Companies which provide poor products and/or poor service have made their own beds and will just have to lie in them. Paul M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From henriks <@t> southbaytech.com Tue Oct 24 13:35:33 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 13:37:40 2006 Subject: [Histonet] Re: goodbye - vendor perspective Message-ID: <453E5CF5.8080105@southbaytech.com> Paul, Mark and Doug: I said nothing about censorship and I did not discourage anyone from sharing their opinion. I suggested that those opinions be shared off-line. Often times there is much more to the story than the initial complaint might indicate. Getting the whole story out would probably involve multiple emails and discussion. A recurrent theme I am hearing is that if the vendor makes a good product and offers good service, then they have nothing to worry about. I don't mean to be flippant, but that is naive. Marketing is the name of the game. Companies with great products and services go under all the time because of negative publicity. Alternatively, companies with marginal products and service often thrive due to extensive and aggressive marketing. In this industry - as in many industries - the products are produced by a large group of small companies. Many companies don't have a huge marketing budget that would allow them to counteract the negative publicity given on a site like this. How is a vendor to respond? Do you want the vendor to continue a thread on the Histonet providing the details of the individual incident? Is that appropriate? Or, does the vendor ignore it and leave that negative impression hanging in the air to the countless members that have seen it and tucked away that thought in the back of their minds. Or, as has been suggested, do you ban vendors altogether so there is no forum at all for them to respond. As for Doug's comment about making a positive comment, of course I wouldn't mind that. That's why I said "praise in public, criticize in private". There is no harm to a vendor by publicly praising them. Just as there is no harm to the inquirer if they receive criticism about the vendor they are considering in private. I'm sure you wouldn't have a problem if your boss posted to the Histonet that you are the greatest histologist in the history of histology. Would you have a problem if he posted to the Histonet that he had some problems with you and that he questioned your ability to perform your job? If you're a great histologist, I'm sure that it wouldn't bother you to have negative comments posted about you for everyone to see. After all, your work should speak for itself. Maybe some people had never heard of you and were not aware that you are the worlds greatest histologist. Do you think their opinion of you might be skewed a little bit by the negative comment? We are all "vendors" to some degree. We are either selling our talents to our employer or selling our goods to an end user. We are all an integral and equal part of the industry and it is wrong to treat vendors as if they are anything less. Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Douglas D Deltour wrote: >Hear Hear!! > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, >Paul >Sent: Tuesday, October 24, 2006 1:21 PM >To: 'histonet@lists.utsouthwestern.edu' >Subject: RE: [Histonet] Re: goodbye - vendor perspective > >David H., > >While I agree with much of what you said, I nevertheless fully support the >practice of seeking feedback/recommendations - positive or negative - about >an expensive piece of equipment before purchasing it. Obviously, other >users of the equipment are the only reliable source from which such >information can be obtained. Offering a response to such a query does not >constitute "airing grievances". It constitutes sharing of valuable >information which consumers have a right to know. I realize that any >company, even the best, can run into a problem now and then. But I don't see >why that should present a problem. If I request information from users about >a particular product or company, and one person responds negatively - which >he/she has every right to do - while a dozen others report satisfaction, >then of course I will go ahead and try the product or company myself. But if >a majority of people report problems with a product or company, then of >course I'll keep my distance. Companies which consistently provide good >products and good service only stand to gain from such online >recommendations. Companies which provide poor products and/or poor service >have made their own beds and will just have to lie in them. > > >Paul M. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From histo20 <@t> hotmail.com Tue Oct 24 13:51:00 2006 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Tue Oct 24 13:51:20 2006 Subject: [Histonet] Available Saturday/Flex Position available in Towson Message-ID: Hi everyone, Just wanted to post a newly vacated position at St. Joseph Medical Center. We are looking for a Saturday flex (and possibly weekday hours as well) Pathology Assistant to fill in for our fulltime PAs. Hours are somewhat flexible. Please, if anyone is interested, please contact our HR department here at the hospital at 410-337-1288. Thanks so much! Paula Wilder St. Joseph Medical Center Towson, MD 21204 410-337-1741 _________________________________________________________________ Try the next generation of search with Windows Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&source=hmtagline From mcauliff <@t> umdnj.edu Tue Oct 24 13:59:51 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 24 13:59:39 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E3900.4040407@southbaytech.com> References: <20061024143443.30792.qmail@web61215.mail.yahoo.com> <453E3900.4040407@southbaytech.com> Message-ID: <453E62A7.8050404@umdnj.edu> Dear list: David Henriks wrote a very nice-sounding reply to the vendor issue we are all concerned about. I say nice-sounding because while it may be the ideal situation, it is not always a real world situation. Joe's troubles bear this out. Several years ago someone posted a querry on this listserver (or the Microscopy listserver, I am not sure which) about purchasing an expensive piece of equipment. I suggested he invite vendors to bring a demo to his lab to see if their equipment fit his needs. I also suggested that any vendor who would not supply a demo not be considered in his purchase plans. I got a very nasty off list reply from .......... David Henriks of South Bay Technologies. Actions speak louder than words. Geoff David Henriks wrote: > I missed the earliest part of the thread here, but from what I see it > seems that someone criticized a vendor on the Histonet and then was > reprimanded by his boss for doing so after the vendor complained. > Now, everyone wants to ban vendors from the Histonet. I am a vendor > and I belong to many listservers. End users seem to have a very > skewed view of what a vendor is and what motivates us. My main > business is not histology, although we do offer some products for the > industry. Yes, companies are in business to make money. No > question. To say that patient care is not even on their radar screen > is ridiculous. If the customer's need is to improve patient care, > then that is what a vendor is striving for. Vendors make money by > supplying what customers want. If you want tools for better patient > care, then that is what we provide. If your needs change, then our > products change. We respond to your needs. If, as you say, patient > care is not even on the vendor radar screen, then that probably means > that customers have not made that a high priority in what they say > they need. Vendors do not make money by producing ineffective > products that customers don't want. The best way to ensure that > customers get what they need and vendors produce products to meet > those needs is to have an open forum where these issues can be freely > discussed. That requires interchange between end users and vendors. > Yes, there should be limitations - vendors should not overtly market > their products on the Histonet. They should respond to postings with > viable solutions - even if they involve their own products. Vendors > should not use this as a forum to denigrate their competition. By the > same token, end users should not use this as a forum to air their > grievances about a product or a manufacturer. A single negative > comment about a vendor or his product could have a devastating effect > on their business. Perhaps your complaints are justified, but there > are better ways to go about having your concerns heard. Any credible > vendor would love to hear from you if you are having a problem with > one of their products. Unfortunately, most customers do not contact > the company. Sometimes when they do contact a company they reach the > wrong person and do not get a satisfactory response. I can understand > the frustration. However, sharing your frustration in such a public > forum is not right. Just as it would not be right for you to > criticize one of your colleagues on the Histonet. > There are always the few that think vendors are their enemies and > think that they are solely motivated by greed. The reality is that > vendors and end users are in this together. The people that figure > that out early on are generally the most successful and the one who > actually drive the development of new products, new techniques and > make significant strides in their field. To ban vendors from your > site would be regrettable and counterproductive. > > The listserver should be used for a free exchange of ideas and not as > a venue for criticism. Praise in public, criticize in private. > > Can't we all just get along? > > Best regards- > > David > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Malcolm.McCallum <@t> tamut.edu Tue Oct 24 14:02:53 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Tue Oct 24 14:05:24 2006 Subject: [Histonet] sections curling References: Message-ID: Hello, sorry for the simplistic question. We are having a lot of problem with some sections curling and wrinkling. this is decalcified bone in parafin. We are using a sliding microtome with disposable blades. is it the blades? This is my first use of disposables. It seems like the answer is very simple, but I have drawn a blank. VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Paula Wilder Sent: Tue 10/24/2006 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Available Saturday/Flex Position available in Towson Hi everyone, Just wanted to post a newly vacated position at St. Joseph Medical Center. We are looking for a Saturday flex (and possibly weekday hours as well) Pathology Assistant to fill in for our fulltime PAs. Hours are somewhat flexible. Please, if anyone is interested, please contact our HR department here at the hospital at 410-337-1288. Thanks so much! Paula Wilder St. Joseph Medical Center Towson, MD 21204 410-337-1741 _________________________________________________________________ Try the next generation of search with Windows Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&source=hmtagline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Oct 24 14:04:12 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 24 14:06:49 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E48AE.2050505@southbaytech.com> References: <453E48AE.2050505@southbaytech.com> Message-ID: <453E63AC.2040505@umdnj.edu> This is exactly the kind of "I make the rules and you should" concept that started the current thread (and antogonizes customers). Geoff David Henriks wrote: > John should answer Jane's question. > > John should answer Jane's question directly to Jane. > > John should not post his comments to the listserver as a whole. > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From wsimms <@t> mcintoshclinic.com Tue Oct 24 14:08:51 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Tue Oct 24 14:09:02 2006 Subject: [Histonet] Re: goodbye - vendor perspective References: <4EBFF65383B74D49995298C4976D1D5E273BA1@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <00bf01c6f79f$d80bf1c0$530114ac@w3256domain.com> To those who would suggest that Histonet be reserved only for "nice" comments regarding vendors, I would like to point out that disseminating the "nice" version of the facts is the raison d' etre of the vendors' multimillion dollar advertising departments. Allowing the idea-stifling practice of political correctness to ooze into and contaminate a professional forum for the free flow of ideas would be an injustice to the patients in whose care we are entrusted, and we should be collectively ashamed should we permit this to occur. Wesley W. Simms From mcauliff <@t> umdnj.edu Tue Oct 24 14:10:56 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 24 14:15:22 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E5CF5.8080105@southbaytech.com> References: <453E5CF5.8080105@southbaytech.com> Message-ID: <453E6540.3090903@umdnj.edu> David et al: You DID suggest censorship and you DID discourage people from sharing their opinion. Please re-read your post, included below, from earlier today. John should answer Jane's question. John should answer Jane's question directly to Jane. John should not post his comments to the listserver as a whole. Geoff David Henriks wrote: > Paul, Mark and Doug: > > I said nothing about censorship and I did not discourage anyone from > sharing their opinion. I suggested that those opinions be shared > off-line. Often times there is much more to the story than the > initial complaint might indicate. Getting the whole story out would > probably involve multiple emails and discussion. A recurrent theme I > am hearing is that if the vendor makes a good product and offers good > service, then they have nothing to worry about. I don't mean to be > flippant, but that is naive. Marketing is the name of the game. > Companies with great products and services go under all the time > because of negative publicity. Alternatively, companies with marginal > products and service often thrive due to extensive and aggressive > marketing. In this industry - as in many industries - the products > are produced by a large group of small companies. Many companies > don't have a huge marketing budget that would allow them to counteract > the negative publicity given on a site like this. How is a vendor to > respond? Do you want the vendor to continue a thread on the Histonet > providing the details of the individual incident? Is that > appropriate? Or, does the vendor ignore it and leave that negative > impression hanging in the air to the countless members that have seen > it and tucked away that thought in the back of their minds. Or, as > has been suggested, do you ban vendors altogether so there is no forum > at all for them to respond. > > As for Doug's comment about making a positive comment, of course I > wouldn't mind that. That's why I said "praise in public, criticize in > private". There is no harm to a vendor by publicly praising them. > Just as there is no harm to the inquirer if they receive criticism > about the vendor they are considering in private. > > I'm sure you wouldn't have a problem if your boss posted to the > Histonet that you are the greatest histologist in the history of > histology. Would you have a problem if he posted to the Histonet that > he had some problems with you and that he questioned your ability to > perform your job? If you're a great histologist, I'm sure that it > wouldn't bother you to have negative comments posted about you for > everyone to see. After all, your work should speak for itself. Maybe > some people had never heard of you and were not aware that you are the > worlds greatest histologist. Do you think their opinion of you might > be skewed a little bit by the negative comment? > > We are all "vendors" to some degree. We are either selling our > talents to our employer or selling our goods to an end user. We are > all an integral and equal part of the industry and it is wrong to > treat vendors as if they are anything less. > > Best regards- > > David > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From henriks <@t> southbaytech.com Tue Oct 24 14:15:47 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 14:17:55 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E63AC.2040505@umdnj.edu> References: <453E48AE.2050505@southbaytech.com> <453E63AC.2040505@umdnj.edu> Message-ID: <453E6663.6020606@southbaytech.com> Geoff: Perhaps you missed what I was responding to. I have pasted it below: "Jane asks Histonet about product "A" John has used product "A" and has had many documented problems. John responds to Histonet and Jane about product "A". Product "A's" people calls John's boss because they did not like John's comments which are documented and true. So John should not answer Jane's question? Douglas D. Deltour HT(ASCP)" I was responding to a question in the same form in which it was presented. How is it that my response offended you? How would my response possibly antagonize a customer? Where do you get the "I make the rules" part? I'm serious, I really would like you to explain how my response was so offensive and how I would have antagonized customers. Honestly, I really don't get it. Thanks. David - David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Geoff McAuliffe wrote: > This is exactly the kind of "I make the rules and you should" concept > that started the current thread (and antogonizes customers). > > Geoff > > > David Henriks wrote: > >> John should answer Jane's question. >> >> John should answer Jane's question directly to Jane. >> >> John should not post his comments to the listserver as a whole. >> > > From Barry.R.Rittman <@t> uth.tmc.edu Tue Oct 24 14:22:18 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Oct 24 14:22:24 2006 Subject: [Histonet] End Goodbye comments Message-ID: There will never be an ideal solution, lets all use a little common sense and tolerance for both vendors and customers and look at each situation individually as one size solution does not fit all. Sometimes both vendors and customers are hoist by their own petard. It appears to me that it is now time for us to start discussing histology topics again. Barry From leswes <@t> shaw.ca Tue Oct 24 14:32:43 2006 From: leswes <@t> shaw.ca (Lesley Weston) Date: Tue Oct 24 14:34:00 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E48AE.2050505@southbaytech.com> Message-ID: But that way, when Alice, Bob and Maximilian all need the same information that Jane did it won't be available to them, so their patients will suffer because John's freedom of speech has been removed. Perhaps the safest thing to do would be to give enthusiastic positive replies on the forum if such are warranted, and to say "No comment", also on the forum, if one has had problems with that product. I'm retired. My former boss would never have listened to slander as Joe's did, so I'm doubly safe! Lesley Weston. > From: David Henriks > Date: Tue, 24 Oct 2006 10:09:02 -0700 > To: Douglas D Deltour > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: goodbye - vendor perspective > > John should answer Jane's question. > > John should answer Jane's question directly to Jane. > > John should not post his comments to the listserver as a whole. > > -- > David Henriks > President > South Bay Technology, Inc. > 1120 Via Callejon > San Clemente, CA 92673 USA > > TEL: +1-949-492-2600 > Toll-free in the USA: +1-800-728-2233 > FAX: +1-949-492-1499 > > email: henriks@southbaytech.com > > Celebrating 40 years of providing Materials Processing Solutions for > Metallogaphy, Crystallography and Electron Microscopy. > > Please visit us online at www.southbaytech.com. > > The information contained in this message and any attachments is privileged > and confidential. This message is intended for the individual or entity > addressed. If you are not the intended recipient, please do not read, copy or > disclose this communication. Notify the sender of the mistake by calling > +1-949-492-2600 and delete this message from your system. > > > > Douglas D Deltour wrote: > >> Jane asks Histonet about product "A" >> >> John has used product "A" and has had many documented problems. >> >> John responds to Histonet and Jane about product "A". >> >> Product "A's" people calls John's boss because they did not like John's >> comments which are documented and true. >> >> So John should not answer Jane's question? >> >> >> Douglas D. Deltour HT(ASCP) >> Histology Supervisor >> Professional Pathology Services, PC >> One Science Court >> Suite 200 >> Columbia, SC 29203 >> (803)252-1913 >> Fax (803)254-3262 >> >> ***************************************************** >> PROFESSIONAL PATHOLOGY SERVICES, PC >> NOTICE OF CONFIDENTIALITY >> This message is intended only for the use of the individual or entity to >> which it is addressed and may contain information that is privileged, >> confidential and exempt from disclosure under applicable law. If the reader >> of this message is not the intended recipient, you are hereby notified that >> any dissemination, distribution, or copying of this communication is >> strictly prohibited by law. If you have received this communication in >> error, please notify me immediately. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >> Henriks >> Sent: Tuesday, October 24, 2006 12:02 PM >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Re: goodbye - vendor perspective >> >> I missed the earliest part of the thread here, but from what I see it >> seems that someone criticized a vendor on the Histonet and then was >> reprimanded by his boss for doing so after the vendor complained. Now, >> everyone wants to ban vendors from the Histonet. I am a vendor and I >> belong to many listservers. End users seem to have a very skewed view >> of what a vendor is and what motivates us. My main business is not >> histology, although we do offer some products for the industry. Yes, >> companies are in business to make money. No question. To say that >> patient care is not even on their radar screen is ridiculous. If the >> customer's need is to improve patient care, then that is what a vendor >> is striving for. Vendors make money by supplying what customers want. >> If you want tools for better patient care, then that is what we >> provide. If your needs change, then our products change. We respond to >> your needs. If, as you say, patient care is not even on the vendor >> radar screen, then that probably means that customers have not made that >> a high priority in what they say they need. Vendors do not make money >> by producing ineffective products that customers don't want. The best >> way to ensure that customers get what they need and vendors produce >> products to meet those needs is to have an open forum where these issues >> can be freely discussed. That requires interchange between end users >> and vendors. >> >> Yes, there should be limitations - vendors should not overtly market >> their products on the Histonet. They should respond to postings with >> viable solutions - even if they involve their own products. Vendors >> should not use this as a forum to denigrate their competition. By the >> same token, end users should not use this as a forum to air their >> grievances about a product or a manufacturer. A single negative comment >> about a vendor or his product could have a devastating effect on their >> business. Perhaps your complaints are justified, but there are better >> ways to go about having your concerns heard. Any credible vendor would >> love to hear from you if you are having a problem with one of their >> products. Unfortunately, most customers do not contact the company. >> Sometimes when they do contact a company they reach the wrong person and >> do not get a satisfactory response. I can understand the frustration. >> However, sharing your frustration in such a public forum is not right. >> Just as it would not be right for you to criticize one of your >> colleagues on the Histonet. >> >> There are always the few that think vendors are their enemies and think >> that they are solely motivated by greed. The reality is that vendors >> and end users are in this together. The people that figure that out >> early on are generally the most successful and the one who actually >> drive the development of new products, new techniques and make >> significant strides in their field. To ban vendors from your site would >> be regrettable and counterproductive. >> >> The listserver should be used for a free exchange of ideas and not as a >> venue for criticism. Praise in public, criticize in private. >> >> Can't we all just get along? >> >> Best regards- >> >> David >> >> >> > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Oct 24 14:30:06 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 24 14:42:18 2006 Subject: [Histonet]advice to vendors In-Reply-To: <453E5CF5.8080105@southbaytech.com> References: <453E5CF5.8080105@southbaytech.com> Message-ID: <453E69BE.1000609@umdnj.edu> Vendors might want to remember, and practice, this old chestnut ......... "You never win an argument with a customer" Geoff David Henriks wrote: > Paul, Mark and Doug: > > I said nothing about censorship and I did not discourage anyone from > sharing their opinion. I suggested that those opinions be shared > off-line. Often times there is much more to the story than the > initial complaint might indicate. Getting the whole story out would > probably involve multiple emails and discussion. A recurrent theme I > am hearing is that if the vendor makes a good product and offers good > service, then they have nothing to worry about. I don't mean to be > flippant, but that is naive. Marketing is the name of the game. > Companies with great products and services go under all the time > because of negative publicity. Alternatively, companies with marginal > products and service often thrive due to extensive and aggressive > marketing. In this industry - as in many industries - the products > are produced by a large group of small companies. Many companies > don't have a huge marketing budget that would allow them to counteract > the negative publicity given on a site like this. How is a vendor to > respond? Do you want the vendor to continue a thread on the Histonet > providing the details of the individual incident? Is that > appropriate? Or, does the vendor ignore it and leave that negative > impression hanging in the air to the countless members that have seen > it and tucked away that thought in the back of their minds. Or, as > has been suggested, do you ban vendors altogether so there is no forum > at all for them to respond. > > As for Doug's comment about making a positive comment, of course I > wouldn't mind that. That's why I said "praise in public, criticize in > private". There is no harm to a vendor by publicly praising them. > Just as there is no harm to the inquirer if they receive criticism > about the vendor they are considering in private. > > I'm sure you wouldn't have a problem if your boss posted to the > Histonet that you are the greatest histologist in the history of > histology. Would you have a problem if he posted to the Histonet that > he had some problems with you and that he questioned your ability to > perform your job? If you're a great histologist, I'm sure that it > wouldn't bother you to have negative comments posted about you for > everyone to see. After all, your work should speak for itself. Maybe > some people had never heard of you and were not aware that you are the > worlds greatest histologist. Do you think their opinion of you might > be skewed a little bit by the negative comment? > > We are all "vendors" to some degree. We are either selling our > talents to our employer or selling our goods to an end user. We are > all an integral and equal part of the industry and it is wrong to > treat vendors as if they are anything less. > > Best regards- > > David > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From doug <@t> ppspath.com Tue Oct 24 14:46:27 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 14:43:41 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E5CF5.8080105@southbaytech.com> Message-ID: David, My boss posting on histonet? Now you are grasping at straws. Your comparisons have nothing to do with the opinions of sold merchandise or services. "Praise in public, criticize in private". This may be fine for your employees or your kids but I am not buying anything from Mr. Rogers. If a company is not prepared to receive honest public feedback then they really shouldn't be in the business. Now going to the highest bidder: Keeping my opinions to myself. Do I hear one dollar! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 2:36 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu; 'Monfils,Paul' Subject: Re: [Histonet] Re: goodbye - vendor perspective Paul, Mark and Doug: I said nothing about censorship and I did not discourage anyone from sharing their opinion. I suggested that those opinions be shared off-line. Often times there is much more to the story than the initial complaint might indicate. Getting the whole story out would probably involve multiple emails and discussion. A recurrent theme I am hearing is that if the vendor makes a good product and offers good service, then they have nothing to worry about. I don't mean to be flippant, but that is naive. Marketing is the name of the game. Companies with great products and services go under all the time because of negative publicity. Alternatively, companies with marginal products and service often thrive due to extensive and aggressive marketing. In this industry - as in many industries - the products are produced by a large group of small companies. Many companies don't have a huge marketing budget that would allow them to counteract the negative publicity given on a site like this. How is a vendor to respond? Do you want the vendor to continue a thread on the Histonet providing the details of the individual incident? Is that appropriate? Or, does the vendor ignore it and leave that negative impression hanging in the air to the countless members that have seen it and tucked away that thought in the back of their minds. Or, as has been suggested, do you ban vendors altogether so there is no forum at all for them to respond. As for Doug's comment about making a positive comment, of course I wouldn't mind that. That's why I said "praise in public, criticize in private". There is no harm to a vendor by publicly praising them. Just as there is no harm to the inquirer if they receive criticism about the vendor they are considering in private. I'm sure you wouldn't have a problem if your boss posted to the Histonet that you are the greatest histologist in the history of histology. Would you have a problem if he posted to the Histonet that he had some problems with you and that he questioned your ability to perform your job? If you're a great histologist, I'm sure that it wouldn't bother you to have negative comments posted about you for everyone to see. After all, your work should speak for itself. Maybe some people had never heard of you and were not aware that you are the worlds greatest histologist. Do you think their opinion of you might be skewed a little bit by the negative comment? We are all "vendors" to some degree. We are either selling our talents to our employer or selling our goods to an end user. We are all an integral and equal part of the industry and it is wrong to treat vendors as if they are anything less. Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Douglas D Deltour wrote: >Hear Hear!! > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, >Paul >Sent: Tuesday, October 24, 2006 1:21 PM >To: 'histonet@lists.utsouthwestern.edu' >Subject: RE: [Histonet] Re: goodbye - vendor perspective > >David H., > >While I agree with much of what you said, I nevertheless fully support the >practice of seeking feedback/recommendations - positive or negative - about >an expensive piece of equipment before purchasing it. Obviously, other >users of the equipment are the only reliable source from which such >information can be obtained. Offering a response to such a query does not >constitute "airing grievances". It constitutes sharing of valuable >information which consumers have a right to know. I realize that any >company, even the best, can run into a problem now and then. But I don't see >why that should present a problem. If I request information from users about >a particular product or company, and one person responds negatively - which >he/she has every right to do - while a dozen others report satisfaction, >then of course I will go ahead and try the product or company myself. But if >a majority of people report problems with a product or company, then of >course I'll keep my distance. Companies which consistently provide good >products and good service only stand to gain from such online >recommendations. Companies which provide poor products and/or poor service >have made their own beds and will just have to lie in them. > > >Paul M. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Oct 24 14:47:42 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 24 14:43:47 2006 Subject: [Histonet] sections curling In-Reply-To: Message-ID: <001201c6f7a5$452fc1a0$0300a8c0@domain.Premier> How thick are you cutting your sections? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malcolm McCallum Sent: Tuesday, October 24, 2006 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections curling Hello, sorry for the simplistic question. We are having a lot of problem with some sections curling and wrinkling. this is decalcified bone in parafin. We are using a sliding microtome with disposable blades. is it the blades? This is my first use of disposables. It seems like the answer is very simple, but I have drawn a blank. VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Paula Wilder Sent: Tue 10/24/2006 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Available Saturday/Flex Position available in Towson Hi everyone, Just wanted to post a newly vacated position at St. Joseph Medical Center. We are looking for a Saturday flex (and possibly weekday hours as well) Pathology Assistant to fill in for our fulltime PAs. Hours are somewhat flexible. Please, if anyone is interested, please contact our HR department here at the hospital at 410-337-1288. Thanks so much! Paula Wilder St. Joseph Medical Center Towson, MD 21204 410-337-1741 _________________________________________________________________ Try the next generation of search with Windows Live Search today! http://imagine-windowslive.com/minisites/searchlaunch/?locale=en-us&source=h mtagline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1830 (20061024) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From MadaryJ <@t> MedImmune.com Tue Oct 24 14:46:34 2006 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Oct 24 14:47:07 2006 Subject: [Histonet] Have we in fact kicked off vendors? Message-ID: <7CAB706201F11843BD26AD516326F0C801AD28FF@MD1MS007.medimmune.com> I have been able to use the histonet to make big decisions on equipment, and I have also saved time by having vendors approach me offering fair prices BECAUSE of histonet when they saw I was looking. So leave the vendors on, let people say what they want about products. I have to say that unless a person says something bad about a vendor, product discussion is supposed to be uncensored right? I do not want to make a mistake on this great resource. I guess what I am askings is have we kicked off vendors? From doug <@t> ppspath.com Tue Oct 24 14:55:41 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 14:52:52 2006 Subject: [Histonet]advice to vendors In-Reply-To: <453E69BE.1000609@umdnj.edu> Message-ID: Or "you never win a customer with an argument". Sorry I just made it up. I think the horse is on its last leg. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Tuesday, October 24, 2006 3:30 PM To: David Henriks Cc: Douglas D Deltour; histonet@lists.utsouthwestern.edu; 'Monfils, Paul' Subject: Re: [Histonet]advice to vendors Vendors might want to remember, and practice, this old chestnut ......... "You never win an argument with a customer" Geoff David Henriks wrote: > Paul, Mark and Doug: > > I said nothing about censorship and I did not discourage anyone from > sharing their opinion. I suggested that those opinions be shared > off-line. Often times there is much more to the story than the > initial complaint might indicate. Getting the whole story out would > probably involve multiple emails and discussion. A recurrent theme I > am hearing is that if the vendor makes a good product and offers good > service, then they have nothing to worry about. I don't mean to be > flippant, but that is naive. Marketing is the name of the game. > Companies with great products and services go under all the time > because of negative publicity. Alternatively, companies with marginal > products and service often thrive due to extensive and aggressive > marketing. In this industry - as in many industries - the products > are produced by a large group of small companies. Many companies > don't have a huge marketing budget that would allow them to counteract > the negative publicity given on a site like this. How is a vendor to > respond? Do you want the vendor to continue a thread on the Histonet > providing the details of the individual incident? Is that > appropriate? Or, does the vendor ignore it and leave that negative > impression hanging in the air to the countless members that have seen > it and tucked away that thought in the back of their minds. Or, as > has been suggested, do you ban vendors altogether so there is no forum > at all for them to respond. > > As for Doug's comment about making a positive comment, of course I > wouldn't mind that. That's why I said "praise in public, criticize in > private". There is no harm to a vendor by publicly praising them. > Just as there is no harm to the inquirer if they receive criticism > about the vendor they are considering in private. > > I'm sure you wouldn't have a problem if your boss posted to the > Histonet that you are the greatest histologist in the history of > histology. Would you have a problem if he posted to the Histonet that > he had some problems with you and that he questioned your ability to > perform your job? If you're a great histologist, I'm sure that it > wouldn't bother you to have negative comments posted about you for > everyone to see. After all, your work should speak for itself. Maybe > some people had never heard of you and were not aware that you are the > worlds greatest histologist. Do you think their opinion of you might > be skewed a little bit by the negative comment? > > We are all "vendors" to some degree. We are either selling our > talents to our employer or selling our goods to an end user. We are > all an integral and equal part of the industry and it is wrong to > treat vendors as if they are anything less. > > Best regards- > > David > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 24 14:52:45 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 14:52:57 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E5CF5.8080105@southbaytech.com> Message-ID: <20061024195245.71909.qmail@web61212.mail.yahoo.com> David: If I have a question I would like to receive as many answers as possible and if those answers are posted for all to see, maybe somebody with the same question will benefit from the answers. That is the reasoning behind a recommendation when you join Histonet: to address your answers to all, not just to the person asking so all can benefit. It is somewhat disingenuous to accep public positive comments and reject public criticisms, although it is understandable from a vendor's point of view. This is a public forum for all to benefit. The honest thing is to recognize the problems, APOLOGIZE if necessary, and move on! Ren? J. David Henriks wrote: Paul, Mark and Doug: I said nothing about censorship and I did not discourage anyone from sharing their opinion. I suggested that those opinions be shared off-line. Often times there is much more to the story than the initial complaint might indicate. Getting the whole story out would probably involve multiple emails and discussion. A recurrent theme I am hearing is that if the vendor makes a good product and offers good service, then they have nothing to worry about. I don't mean to be flippant, but that is naive. Marketing is the name of the game. Companies with great products and services go under all the time because of negative publicity. Alternatively, companies with marginal products and service often thrive due to extensive and aggressive marketing. In this industry - as in many industries - the products are produced by a large group of small companies. Many companies don't have a huge marketing budget that would allow them to counteract the negative publicity given on a site like this. How is a vendor to respond? Do you want the vendor to continue a thread on the Histonet providing the details of the individual incident? Is that appropriate? Or, does the vendor ignore it and leave that negative impression hanging in the air to the countless members that have seen it and tucked away that thought in the back of their minds. Or, as has been suggested, do you ban vendors altogether so there is no forum at all for them to respond. As for Doug's comment about making a positive comment, of course I wouldn't mind that. That's why I said "praise in public, criticize in private". There is no harm to a vendor by publicly praising them. Just as there is no harm to the inquirer if they receive criticism about the vendor they are considering in private. I'm sure you wouldn't have a problem if your boss posted to the Histonet that you are the greatest histologist in the history of histology. Would you have a problem if he posted to the Histonet that he had some problems with you and that he questioned your ability to perform your job? If you're a great histologist, I'm sure that it wouldn't bother you to have negative comments posted about you for everyone to see. After all, your work should speak for itself. Maybe some people had never heard of you and were not aware that you are the worlds greatest histologist. Do you think their opinion of you might be skewed a little bit by the negative comment? We are all "vendors" to some degree. We are either selling our talents to our employer or selling our goods to an end user. We are all an integral and equal part of the industry and it is wrong to treat vendors as if they are anything less. Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Douglas D Deltour wrote: >Hear Hear!! > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, >Paul >Sent: Tuesday, October 24, 2006 1:21 PM >To: 'histonet@lists.utsouthwestern.edu' >Subject: RE: [Histonet] Re: goodbye - vendor perspective > >David H., > >While I agree with much of what you said, I nevertheless fully support the >practice of seeking feedback/recommendations - positive or negative - about >an expensive piece of equipment before purchasing it. Obviously, other >users of the equipment are the only reliable source from which such >information can be obtained. Offering a response to such a query does not >constitute "airing grievances". It constitutes sharing of valuable >information which consumers have a right to know. I realize that any >company, even the best, can run into a problem now and then. But I don't see >why that should present a problem. If I request information from users about >a particular product or company, and one person responds negatively - which >he/she has every right to do - while a dozen others report satisfaction, >then of course I will go ahead and try the product or company myself. But if >a majority of people report problems with a product or company, then of >course I'll keep my distance. Companies which consistently provide good >products and good service only stand to gain from such online >recommendations. Companies which provide poor products and/or poor service >have made their own beds and will just have to lie in them. > > >Paul M. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From Jackie.O'Connor <@t> abbott.com Tue Oct 24 14:58:14 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Oct 24 14:58:52 2006 Subject: [Histonet]advice to vendors In-Reply-To: <453E69BE.1000609@umdnj.edu> Message-ID: I attended a customer satisfaction management seminar a few years ago, where I learned that for every bad experience, a customer will tell approximately 10 people about the problem. However, that same person will tell between 0-2 people about a positive experience with a merchant. Ergo the saying (which my husband used frequently during his 23 year military career) changed to a PG rating - - "It only takes one "AH - Shoot!!" to wipe out TEN "'Atta-boy!"(s) Geoff McAuliffe Sent by: histonet-bounces@lists.utsouthwestern.edu 10/24/2006 02:30 PM To David Henriks cc Douglas D Deltour , histonet@lists.utsouthwestern.edu, "'Monfils, Paul'" Subject Re: [Histonet]advice to vendors Vendors might want to remember, and practice, this old chestnut ......... "You never win an argument with a customer" Geoff David Henriks wrote: > Paul, Mark and Doug: > > I said nothing about censorship and I did not discourage anyone from > sharing their opinion. I suggested that those opinions be shared > off-line. Often times there is much more to the story than the > initial complaint might indicate. Getting the whole story out would > probably involve multiple emails and discussion. A recurrent theme I > am hearing is that if the vendor makes a good product and offers good > service, then they have nothing to worry about. I don't mean to be > flippant, but that is naive. Marketing is the name of the game. > Companies with great products and services go under all the time > because of negative publicity. Alternatively, companies with marginal > products and service often thrive due to extensive and aggressive > marketing. In this industry - as in many industries - the products > are produced by a large group of small companies. Many companies > don't have a huge marketing budget that would allow them to counteract > the negative publicity given on a site like this. How is a vendor to > respond? Do you want the vendor to continue a thread on the Histonet > providing the details of the individual incident? Is that > appropriate? Or, does the vendor ignore it and leave that negative > impression hanging in the air to the countless members that have seen > it and tucked away that thought in the back of their minds. Or, as > has been suggested, do you ban vendors altogether so there is no forum > at all for them to respond. > > As for Doug's comment about making a positive comment, of course I > wouldn't mind that. That's why I said "praise in public, criticize in > private". There is no harm to a vendor by publicly praising them. > Just as there is no harm to the inquirer if they receive criticism > about the vendor they are considering in private. > > I'm sure you wouldn't have a problem if your boss posted to the > Histonet that you are the greatest histologist in the history of > histology. Would you have a problem if he posted to the Histonet that > he had some problems with you and that he questioned your ability to > perform your job? If you're a great histologist, I'm sure that it > wouldn't bother you to have negative comments posted about you for > everyone to see. After all, your work should speak for itself. Maybe > some people had never heard of you and were not aware that you are the > worlds greatest histologist. Do you think their opinion of you might > be skewed a little bit by the negative comment? > > We are all "vendors" to some degree. We are either selling our > talents to our employer or selling our goods to an end user. We are > all an integral and equal part of the industry and it is wrong to > treat vendors as if they are anything less. > > Best regards- > > David > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Oct 24 15:16:13 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Oct 24 15:16:25 2006 Subject: [Histonet] A Bit of Good News Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB2DF@nmdamailsvr.nmda.ad.nmsu.edu> Just a quick post to say THANK YOU to all the histologist-members and vendor-sponsors that helped make the 2006 New Mexico Society for Histology meeting such a nice success! Even after a three-year hiatus, we had twenty-eight techs (one came from Las Vegas, NV!) and the NSH Region VI Director come to our meeting. Twenty-two vendors were represented - either in person or by sending materials for display/giveaway. We have a new Board, some new members and some new enthusiasm for the profession and for our Society. The vendors will be thanked directly, but I just wanted to give recognition (albeit anonymous at this point) to those that gave us their support and allowed us to get "up and running". It looks like the 2007 NMSH meeting (our 15th anniversary) will be held in May (to be stamped in stone later this year) and will be even bigger! Thanks to the histologists that came from the four corners of our State to show that they want this Society to be active! Website coming soon (as soon as I get a 12-year old to help me figure out how to do it - along with our TV remotes and digital clocks...). To be added to next year's mailing list for the NMSH Annual Meeting, contact me at nmhisto@aol.com. Thanks, everyone! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From GDawson <@t> dynacaremilwaukee.com Tue Oct 24 15:24:13 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Oct 24 15:24:28 2006 Subject: [Histonet] End Goodbye comments Message-ID: Here here Barry!! Another topic goes awry. I think I'd have to write a bunch of you up for bagging off on the histonet all day if you were working for me. Rene, I was happy to read that you are retired when I caught up on all of these messages because you'd have been written up twice...at least. Time for a sedative washed down with some absolute Vodka for all. Everyone just seems so angry...it must be the rap music. Happy Happy Joy Joy, Glen Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Tuesday, October 24, 2006 1:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] End Goodbye comments There will never be an ideal solution, lets all use a little common sense and tolerance for both vendors and customers and look at each situation individually as one size solution does not fit all. Sometimes both vendors and customers are hoist by their own petard. It appears to me that it is now time for us to start discussing histology topics again. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From henriks <@t> southbaytech.com Tue Oct 24 15:33:57 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 15:36:02 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E62A7.8050404@umdnj.edu> References: <20061024143443.30792.qmail@web61215.mail.yahoo.com> <453E3900.4040407@southbaytech.com> <453E62A7.8050404@umdnj.edu> Message-ID: <453E78B5.1010708@southbaytech.com> Dear List: I now understand why Geoff was so offended by my harmless comments. This also makes my point as to why it is so important NOT to post your negative opinions about a company - or an individual - on the listserver. You can read the messages below which were retrieved from the Microscopy listserver archives. My message to Geoff was not "off-list" it was sent to him and the listserver. I'm confident that once you read my message to him you will see that it was not nasty at all. There is always more to the story than the single line some disgruntled and frustrated poster may choose to share with you. Geoff has obviously been stewing over my message for more than 3 years! Geoff's own reference makes my case for me. This was posted on the Microscopy Listserver: From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) Subject: LM - question on inverted microscope, Hoffman and VAREL contrast Dear users of ListServer, I kindly ask you for your advice concerning inverted microscope. We need to ....... Therefore, we consider to purchase an inverted microscope but we are not sure if to ask for that one which is equipped with relief contrasting method. If yes, which would be the best? Hoffman modulation ...... As we do not have a possibility to try inverted microscope with relief contrast, I would very appreciate your opinion/advice. Thank you. Rostislav Zemek Czech Republic E-mail: rosta-at-acarus.entu.cas.cz From: Geoff McAuliffe : mcauliff-at-umdnj.edu Date: Tue, 04 Mar 2003 11:58:32 -0500 Subject: Re: LM - question on inverted microscope, Hoffman and VAREL contrast Ask the vendor to bring the instrument you are considering to your lab for a demonstration. We always do this for expensive items. Any vendor who will not comply is not worth dealing with. Geoff From: David Henriks : henriks-at-southbaytech.com Date: Tue, 04 Mar 2003 18:26:26 -0800 Subject: Re: LM - question on inverted microscope, Hoffman and VAREL contrast Geoff: I can appreciate that anyone would prefer to have a vendor deliver an instrument to their door to do an on-site demonstration, but you also must realize that it is simply not possible in every instance. To say that "Any vendor who will not comply is not worth dealing with" is quite simplistic and offensive. I have nothing to do with this particular customer, but I can certainly understand how several excellent microscopy vendors may have difficulty justifying an on-site demo in the Czech Republic. I personally have been to the Czech Republic several times to demonstrate our systems, however, it can certainly not be justified in every instance. To dismiss vendors so capriciously would be doing a disservice to Dr. Zemek. Perhaps the company offering the best solution at the best price has a better method of demonstrating its performance than through an on-site demo. Technology these days is pretty impressive. Speaking as a family business owner and microscopy vendor for nearly 40 years, I guess I take it a bit personally when vendors are treated with such disrespect. Scientists and vendors working together rather than as adversaries is much more productive and serves the scientific community much more effectively. Best regards- David From: Geoff McAuliffe : mcauliff-at-umdnj.edu Date: Wed, 05 Mar 2003 09:56:45 -0500 Subject: Re: LM - question on inverted microscope, Hoffman and VAREL contrast Hi David: David Henriks wrote: } Geoff: } } I can appreciate that anyone would prefer to have a vendor deliver an } instrument to their door to do an on-site demonstration, but you also } must realize that it is simply not possible in every instance. That may be true. } To say } that "Any vendor who will not comply is not worth dealing with" is quite } simplistic and offensive. Hey, times are very tight here in University research land. We spend our money the way we want to. We can't afford to buy equipment we have not tested. If this offends you, so be it. In 30 years in university science, I have never had a vendor refuse to demo a product, even an inexpensive one. } I have nothing to do with this particular } customer, but I can certainly understand how several excellent } microscopy vendors may have difficulty justifying an on-site demo in the } Czech Republic. If that is the nature of their business, then that is the nature of their business. The vendor runs his business the way he wants to, the customer spends his money the way he wants to. Capitalism. } I personally have been to the Czech Republic several } times to demonstrate our systems, however, it can certainly not be } justified in every instance. To dismiss vendors so capriciously would } be doing a disservice to Dr. Zemek. No, Dr Zemek can make his own decisions. I was suggesting one course of action for his consideration. } Perhaps the company offering the } best solution at the best price has a better method of demonstrating its } performance than through an on-site demo. Technology these days is } pretty impressive.Speaking as a family business owner and microscopy vendor for } nearly 40 } years, I guess I take it a bit personally when vendors are treated with } such disrespect. Not disrespect, it is just the way things operate in my world. How is 'not buying a product without a demo to see if it fits your lab's needs' disrespectful?? } Scientists and vendors working together rather than as } adversaries is much more productive and serves the scientific community } much more effectively. That is a very nice, general statement that no one would argue with. It is also 180 degrees opposite of buying a product without knowing if it will solve your problem. Geoff Geoff McAuliffe wrote: > Dear list: > > David Henriks wrote a very nice-sounding reply to the vendor issue > we are all concerned about. I say nice-sounding because while it may > be the ideal situation, it is not always a real world situation. Joe's > troubles bear this out. > Several years ago someone posted a querry on this listserver (or > the Microscopy listserver, I am not sure which) about purchasing an > expensive piece of equipment. I suggested he invite vendors to bring a > demo to his lab to see if their equipment fit his needs. I also > suggested that any vendor who would not supply a demo not be > considered in his purchase plans. I got a very nasty off list reply > from .......... David Henriks of South Bay Technologies. > Actions speak louder than words. > > Geoff > > David Henriks wrote: > >> I missed the earliest part of the thread here, but from what I see it >> seems that someone criticized a vendor on the Histonet and then was >> reprimanded by his boss for doing so after the vendor complained. >> Now, everyone wants to ban vendors from the Histonet. I am a vendor >> and I belong to many listservers. End users seem to have a very >> skewed view of what a vendor is and what motivates us. My main >> business is not histology, although we do offer some products for the >> industry. Yes, companies are in business to make money. No >> question. To say that patient care is not even on their radar screen >> is ridiculous. If the customer's need is to improve patient care, >> then that is what a vendor is striving for. Vendors make money by >> supplying what customers want. If you want tools for better patient >> care, then that is what we provide. If your needs change, then our >> products change. We respond to your needs. If, as you say, patient >> care is not even on the vendor radar screen, then that probably means >> that customers have not made that a high priority in what they say >> they need. Vendors do not make money by producing ineffective >> products that customers don't want. The best way to ensure that >> customers get what they need and vendors produce products to meet >> those needs is to have an open forum where these issues can be freely >> discussed. That requires interchange between end users and vendors. >> Yes, there should be limitations - vendors should not overtly market >> their products on the Histonet. They should respond to postings with >> viable solutions - even if they involve their own products. Vendors >> should not use this as a forum to denigrate their competition. By >> the same token, end users should not use this as a forum to air their >> grievances about a product or a manufacturer. A single negative >> comment about a vendor or his product could have a devastating effect >> on their business. Perhaps your complaints are justified, but there >> are better ways to go about having your concerns heard. Any credible >> vendor would love to hear from you if you are having a problem with >> one of their products. Unfortunately, most customers do not contact >> the company. Sometimes when they do contact a company they reach the >> wrong person and do not get a satisfactory response. I can >> understand the frustration. However, sharing your frustration in >> such a public forum is not right. Just as it would not be right for >> you to criticize one of your colleagues on the Histonet. >> There are always the few that think vendors are their enemies and >> think that they are solely motivated by greed. The reality is that >> vendors and end users are in this together. The people that figure >> that out early on are generally the most successful and the one who >> actually drive the development of new products, new techniques and >> make significant strides in their field. To ban vendors from your >> site would be regrettable and counterproductive. >> >> The listserver should be used for a free exchange of ideas and not as >> a venue for criticism. Praise in public, criticize in private. >> >> Can't we all just get along? >> >> Best regards- >> >> David >> > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. From rjbuesa <@t> yahoo.com Tue Oct 24 15:38:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 24 15:38:16 2006 Subject: Fwd: Re: [Histonet] Re: goodbye - vendor perspective Message-ID: <20061024203805.71253.qmail@web61216.mail.yahoo.com> Rene J Buesa wrote: Date: Tue, 24 Oct 2006 12:52:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Re: goodbye - vendor perspective To: David Henriks , Douglas D Deltour CC: histonet@lists.utsouthwestern.edu, "'Monfils, Paul'" David: If I have a question I would like to receive as many answers as possible and if those answers are posted for all to see, maybe somebody with the same question will benefit from the answers. That is the reasoning behind a recommendation when you join Histonet: to address your answers to all, not just to the person asking so all can benefit. It is somewhat disingenuous to accep public positive comments and reject public criticisms, although it is understandable from a vendor's point of view. This is a public forum for all to benefit. The honest thing is to recognize the problems, APOLOGIZE if necessary, and move on! Ren? J. David Henriks wrote: Paul, Mark and Doug: I said nothing about censorship and I did not discourage anyone from sharing their opinion. I suggested that those opinions be shared off-line. Often times there is much more to the story than the initial complaint might indicate. Getting the whole story out would probably involve multiple emails and discussion. A recurrent theme I am hearing is that if the vendor makes a good product and offers good service, then they have nothing to worry about. I don't mean to be flippant, but that is naive. Marketing is the name of the game. Companies with great products and services go under all the time because of negative publicity. Alternatively, companies with marginal products and service often thrive due to extensive and aggressive marketing. In this industry - as in many industries - the products are produced by a large group of small companies. Many companies don't have a huge marketing budget that would allow them to counteract the negative publicity given on a site like this. How is a vendor to respond? Do you want the vendor to continue a thread on the Histonet providing the details of the individual incident? Is that appropriate? Or, does the vendor ignore it and leave that negative impression hanging in the air to the countless members that have seen it and tucked away that thought in the back of their minds. Or, as has been suggested, do you ban vendors altogether so there is no forum at all for them to respond. As for Doug's comment about making a positive comment, of course I wouldn't mind that. That's why I said "praise in public, criticize in private". There is no harm to a vendor by publicly praising them. Just as there is no harm to the inquirer if they receive criticism about the vendor they are considering in private. I'm sure you wouldn't have a problem if your boss posted to the Histonet that you are the greatest histologist in the history of histology. Would you have a problem if he posted to the Histonet that he had some problems with you and that he questioned your ability to perform your job? If you're a great histologist, I'm sure that it wouldn't bother you to have negative comments posted about you for everyone to see. After all, your work should speak for itself. Maybe some people had never heard of you and were not aware that you are the worlds greatest histologist. Do you think their opinion of you might be skewed a little bit by the negative comment? We are all "vendors" to some degree. We are either selling our talents to our employer or selling our goods to an end user. We are all an integral and equal part of the industry and it is wrong to treat vendors as if they are anything less. Best regards- David -- David Henriks President South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. Douglas D Deltour wrote: >Hear Hear!! > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, >Paul >Sent: Tuesday, October 24, 2006 1:21 PM >To: 'histonet@lists.utsouthwestern.edu' >Subject: RE: [Histonet] Re: goodbye - vendor perspective > >David H., > >While I agree with much of what you said, I nevertheless fully support the >practice of seeking feedback/recommendations - positive or negative - about >an expensive piece of equipment before purchasing it. Obviously, other >users of the equipment are the only reliable source from which such >information can be obtained. Offering a response to such a query does not >constitute "airing grievances". It constitutes sharing of valuable >information which consumers have a right to know. I realize that any >company, even the best, can run into a problem now and then. But I don't see >why that should present a problem. If I request information from users about >a particular product or company, and one person responds negatively - which >he/she has every right to do - while a dozen others report satisfaction, >then of course I will go ahead and try the product or company myself. But if >a majority of people report problems with a product or company, then of >course I'll keep my distance. Companies which consistently provide good >products and good service only stand to gain from such online >recommendations. Companies which provide poor products and/or poor service >have made their own beds and will just have to lie in them. > > >Paul M. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From henriks <@t> southbaytech.com Tue Oct 24 16:01:01 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 16:03:02 2006 Subject: [Histonet] Re: goodbye - vendor perspective - My final comment In-Reply-To: <453E6E99.50100@umdnj.edu> References: <453E48AE.2050505@southbaytech.com> <453E63AC.2040505@umdnj.edu> <453E6663.6020606@southbaytech.com> <453E6E99.50100@umdnj.edu> Message-ID: <453E7F0D.9060906@southbaytech.com> Geoff: I never intended to start an argument. I thought sharing my opinion was what the listserver was for. Is it not? Are you trying to suggest that I shouldn't share my opinions? I never said a single negative thing about anyone or any company. Your response was to attack me based on your misreading of a 3 year old email exchange. How am I hurting myself and my business by sharing a reasonable and well thought out opinion? Another "old chestnut" vendors may want to remember and practice: "Sometimes you have to fire your customer." Geoff, you're fired. David Vendors might want to remember, and practice, this old chestnut ......... "You never win an argument with a customer" Geoff Geoff McAuliffe wrote: > Hi David: > > You are correct, I did not see the message copied below from Doug > Deltour. What I did see was your statement which I copied in one of my > posts, included here for clarity in italics: > > John should answer Jane's question. > > John should answer Jane's question directly to Jane. > > John should not post his comments to the listserver as a whole. > > This statement came from you. You are not the arbiter of what we > "should" do on Histonet. > Telling us what we should do and should not do is an attempt at > censorship. > You are right, you don't get it. Seriously. > You are hurting yourself and your business by trying to tell us what > we should and should not do. > > > Geoff > > > David Henriks wrote: > >> Geoff: >> >> Perhaps you missed what I was responding to. I have pasted it below: >> >> "Jane asks Histonet about product "A" >> John has used product "A" and has had many documented problems. >> >> John responds to Histonet and Jane about product "A". >> >> Product "A's" people calls John's boss because they did not like John's >> comments which are documented and true. >> >> So John should not answer Jane's question? >> >> Douglas D. Deltour HT(ASCP)" >> >> I was responding to a question in the same form in which it was >> presented. How is it that my response offended you? How would my >> response possibly antagonize a customer? Where do you get the "I >> make the rules" part? >> I'm serious, I really would like you to explain how my response was >> so offensive and how I would have antagonized customers. Honestly, I >> really don't get it. >> >> Thanks. >> >> David >> >> - >> >> David Henriks >> President >> South Bay Technology, Inc. >> 1120 Via Callejon >> San Clemente, CA 92673 USA >> >> TEL: +1-949-492-2600 >> Toll-free in the USA: +1-800-728-2233 >> FAX: +1-949-492-1499 >> >> email: henriks@southbaytech.com >> >> Celebrating 40 years of providing Materials Processing Solutions for >> Metallogaphy, Crystallography and Electron Microscopy. >> Please visit us online at www.southbaytech.com. >> >> The information contained in this message and any attachments is >> privileged and confidential. This message is intended for the >> individual or entity addressed. If you are not the intended >> recipient, please do not read, copy or disclose this communication. >> Notify the sender of the mistake by calling +1-949-492-2600 and >> delete this message from your system. >> >> >> >> Geoff McAuliffe wrote: >> >>> This is exactly the kind of "I make the rules and you should" >>> concept that started the current thread (and antogonizes customers). >>> >>> Geoff >>> >>> >>> David Henriks wrote: >>> >>>> John should answer Jane's question. >>>> >>>> John should answer Jane's question directly to Jane. >>>> >>>> John should not post his comments to the listserver as a whole. >>>> >>> >>> >> >> >> >> >> > > >-- >-- >********************************************** >Geoff McAuliffe, Ph.D. >Neuroscience and Cell Biology >Robert Wood Johnson Medical School >675 Hoes Lane, Piscataway, NJ 08854 >voice: (732)-235-4583; fax: -4029 >mcauliff@umdnj.edu >********************************************** > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. From kmw_htls <@t> hotmail.com Tue Oct 24 16:09:10 2006 From: kmw_htls <@t> hotmail.com (Kallye Walther) Date: Tue Oct 24 16:09:31 2006 Subject: [Histonet] End Goodbye comments In-Reply-To: Message-ID: Well said Barry. For two days now I have seen a lot of emails on this hot topic. What I haven't seen is any more responses from Joe. I guess Joe has resovled the issue. It would be nice if the rest of us could do the same. Here's my thought, ( had to get it there ), The histonet was created for histology related issues, by allowing vendors to comment does benefit us. If a particular vendor has ABUSED this priviledge, then that is a matter for Linda M. and staff. By not allowing vendors to comment there WILL BE ISSUES that will be missed. I am NOT a vendor, but I deal with them daily as I am sure all of us do. Passwords are redundant, not just anyone visits Histonet, only histology related personel. The Histonet is a very valuable TOOL for histology. It does allow all of us to ask questions about issues that we obviously need advice on. It allows all of us to benefit from the experience of another. Of all the histology people subscribing on the Histonet, pooled that experience is probably hundreds of years. There is always something new out there. Without the freedom of uncensored speech, would this country even be here??? Isn;t that part of the reason we left that little island ( England ) a feww hundred years ago? Histonet allows all of us to benefit. Now I don't believe it should be used as a personal attack on anyone tech or vendor, but I sure as H--- don't think it should be turned into a political forum for tech vs. vendor either. This particular vendor should not have gone to Joe's boss, and in return Joe's boss, should not have repremanded him either. Why? other than the fact it is just wrong, how about.... the kick back law??? meaning, if Joe's boss would not stand behind Joe, and took sides of a vendor, the officiating powers that be that regulate kick backs, can certainly ask a few questions as to why???? This might be a bit of a stretch here...... but so far, so has the rest of this particular forum. I t is easy to see the outrage, as a fellow tech and supervisor, I AM outraged!!!! But since we histotechs stick together and will support each other, isn't it possible to think that there are 2 sides to every story, as well as pictures being painted in one's own color??? We all know Joe to be level headed, and distribute honest and sincere advise, so naturally we are all outraged. And while the rest of us are screaming foul.... Joe is certainly appreciating the support, however is would appear that this issue has been resolved with him,....... not here though. Just one tech's opinion. Thank you all for the comments, perspectives, and advice on this matter. Again this is what the Histonet WAS created for. Right Linda M????? >From: "Rittman, Barry R" >To: >Subject: [Histonet] End Goodbye comments >Date: Tue, 24 Oct 2006 14:22:18 -0500 > >There will never be an ideal solution, lets all use a little common >sense and tolerance for both vendors and customers and look at each >situation individually as one size solution does not fit all. > >Sometimes both vendors and customers are hoist by their own petard. > > > >It appears to me that it is now time for us to start discussing >histology topics again. > >Barry > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Stay in touch with old friends and meet new ones with Windows Live Spaces http://clk.atdmt.com/MSN/go/msnnkwsp0070000001msn/direct/01/?href=http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us From doug <@t> ppspath.com Tue Oct 24 16:18:22 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 16:15:41 2006 Subject: [Histonet] Re: goodbye - vendor perspective In-Reply-To: <453E78B5.1010708@southbaytech.com> Message-ID: It seems like you are grasping at straws again. I am sure Geoff is not lying in bed at night stewing over a post three years ago. All he said is that he wouldn't buy a piece of equipment without a demo. He voiced his opinion. He is not a "disgruntled and frustrated poster". It is time to move on. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 4:34 PM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective Dear List: I now understand why Geoff was so offended by my harmless comments. This also makes my point as to why it is so important NOT to post your negative opinions about a company - or an individual - on the listserver. You can read the messages below which were retrieved from the Microscopy listserver archives. My message to Geoff was not "off-list" it was sent to him and the listserver. I'm confident that once you read my message to him you will see that it was not nasty at all. There is always more to the story than the single line some disgruntled and frustrated poster may choose to share with you. Geoff has obviously been stewing over my message for more than 3 years! Geoff's own reference makes my case for me. This was posted on the Microscopy Listserver: From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) Subject: LM - question on inverted microscope, Hoffman and VAREL contrast Dear users of ListServer, I kindly ask you for your advice concerning inverted microscope. We need to ....... Therefore, we consider to purchase an inverted microscope but we are not sure if to ask for that one which is equipped with relief contrasting method. If yes, which would be the best? Hoffman modulation ...... As we do not have a possibility to try inverted microscope with relief contrast, I would very appreciate your opinion/advice. Thank you. Rostislav Zemek Czech Republic E-mail: rosta-at-acarus.entu.cas.cz From: Geoff McAuliffe : mcauliff-at-umdnj.edu Date: Tue, 04 Mar 2003 11:58:32 -0500 Subject: Re: LM - question on inverted microscope, Hoffman and VAREL contrast Ask the vendor to bring the instrument you are considering to your lab for a demonstration. We always do this for expensive items. Any vendor who will not comply is not worth dealing with. Geoff From: David Henriks : henriks-at-southbaytech.com Date: Tue, 04 Mar 2003 18:26:26 -0800 Subject: Re: LM - question on inverted microscope, Hoffman and VAREL contrast Geoff: I can appreciate that anyone would prefer to have a vendor deliver an instrument to their door to do an on-site demonstration, but you also must realize that it is simply not possible in every instance. To say that "Any vendor who will not comply is not worth dealing with" is quite simplistic and offensive. I have nothing to do with this particular customer, but I can certainly understand how several excellent microscopy vendors may have difficulty justifying an on-site demo in the Czech Republic. I personally have been to the Czech Republic several times to demonstrate our systems, however, it can certainly not be justified in every instance. To dismiss vendors so capriciously would be doing a disservice to Dr. Zemek. Perhaps the company offering the best solution at the best price has a better method of demonstrating its performance than through an on-site demo. Technology these days is pretty impressive. Speaking as a family business owner and microscopy vendor for nearly 40 years, I guess I take it a bit personally when vendors are treated with such disrespect. Scientists and vendors working together rather than as adversaries is much more productive and serves the scientific community much more effectively. Best regards- David From: Geoff McAuliffe : mcauliff-at-umdnj.edu Date: Wed, 05 Mar 2003 09:56:45 -0500 Subject: Re: LM - question on inverted microscope, Hoffman and VAREL contrast Hi David: David Henriks wrote: } Geoff: } } I can appreciate that anyone would prefer to have a vendor deliver an } instrument to their door to do an on-site demonstration, but you also } must realize that it is simply not possible in every instance. That may be true. } To say } that "Any vendor who will not comply is not worth dealing with" is quite } simplistic and offensive. Hey, times are very tight here in University research land. We spend our money the way we want to. We can't afford to buy equipment we have not tested. If this offends you, so be it. In 30 years in university science, I have never had a vendor refuse to demo a product, even an inexpensive one. } I have nothing to do with this particular } customer, but I can certainly understand how several excellent } microscopy vendors may have difficulty justifying an on-site demo in the } Czech Republic. If that is the nature of their business, then that is the nature of their business. The vendor runs his business the way he wants to, the customer spends his money the way he wants to. Capitalism. } I personally have been to the Czech Republic several } times to demonstrate our systems, however, it can certainly not be } justified in every instance. To dismiss vendors so capriciously would } be doing a disservice to Dr. Zemek. No, Dr Zemek can make his own decisions. I was suggesting one course of action for his consideration. } Perhaps the company offering the } best solution at the best price has a better method of demonstrating its } performance than through an on-site demo. Technology these days is } pretty impressive.Speaking as a family business owner and microscopy vendor for } nearly 40 } years, I guess I take it a bit personally when vendors are treated with } such disrespect. Not disrespect, it is just the way things operate in my world. How is 'not buying a product without a demo to see if it fits your lab's needs' disrespectful?? } Scientists and vendors working together rather than as } adversaries is much more productive and serves the scientific community } much more effectively. That is a very nice, general statement that no one would argue with. It is also 180 degrees opposite of buying a product without knowing if it will solve your problem. Geoff Geoff McAuliffe wrote: > Dear list: > > David Henriks wrote a very nice-sounding reply to the vendor issue > we are all concerned about. I say nice-sounding because while it may > be the ideal situation, it is not always a real world situation. Joe's > troubles bear this out. > Several years ago someone posted a querry on this listserver (or > the Microscopy listserver, I am not sure which) about purchasing an > expensive piece of equipment. I suggested he invite vendors to bring a > demo to his lab to see if their equipment fit his needs. I also > suggested that any vendor who would not supply a demo not be > considered in his purchase plans. I got a very nasty off list reply > from .......... David Henriks of South Bay Technologies. > Actions speak louder than words. > > Geoff > > David Henriks wrote: > >> I missed the earliest part of the thread here, but from what I see it >> seems that someone criticized a vendor on the Histonet and then was >> reprimanded by his boss for doing so after the vendor complained. >> Now, everyone wants to ban vendors from the Histonet. I am a vendor >> and I belong to many listservers. End users seem to have a very >> skewed view of what a vendor is and what motivates us. My main >> business is not histology, although we do offer some products for the >> industry. Yes, companies are in business to make money. No >> question. To say that patient care is not even on their radar screen >> is ridiculous. If the customer's need is to improve patient care, >> then that is what a vendor is striving for. Vendors make money by >> supplying what customers want. If you want tools for better patient >> care, then that is what we provide. If your needs change, then our >> products change. We respond to your needs. If, as you say, patient >> care is not even on the vendor radar screen, then that probably means >> that customers have not made that a high priority in what they say >> they need. Vendors do not make money by producing ineffective >> products that customers don't want. The best way to ensure that >> customers get what they need and vendors produce products to meet >> those needs is to have an open forum where these issues can be freely >> discussed. That requires interchange between end users and vendors. >> Yes, there should be limitations - vendors should not overtly market >> their products on the Histonet. They should respond to postings with >> viable solutions - even if they involve their own products. Vendors >> should not use this as a forum to denigrate their competition. By >> the same token, end users should not use this as a forum to air their >> grievances about a product or a manufacturer. A single negative >> comment about a vendor or his product could have a devastating effect >> on their business. Perhaps your complaints are justified, but there >> are better ways to go about having your concerns heard. Any credible >> vendor would love to hear from you if you are having a problem with >> one of their products. Unfortunately, most customers do not contact >> the company. Sometimes when they do contact a company they reach the >> wrong person and do not get a satisfactory response. I can >> understand the frustration. However, sharing your frustration in >> such a public forum is not right. Just as it would not be right for >> you to criticize one of your colleagues on the Histonet. >> There are always the few that think vendors are their enemies and >> think that they are solely motivated by greed. The reality is that >> vendors and end users are in this together. The people that figure >> that out early on are generally the most successful and the one who >> actually drive the development of new products, new techniques and >> make significant strides in their field. To ban vendors from your >> site would be regrettable and counterproductive. >> >> The listserver should be used for a free exchange of ideas and not as >> a venue for criticism. Praise in public, criticize in private. >> >> Can't we all just get along? >> >> Best regards- >> >> David >> > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Oct 24 16:31:03 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Oct 24 16:28:09 2006 Subject: [Histonet] End Goodbye comments-Tomorrow! In-Reply-To: Message-ID: As I pack up my bags and get ready to head out, I want to dedicate a song to all of the hard working histotechs and vendors out there... The sun'll come out Tomorrow So ya gotta hang on 'Til tomorrow Come what may Tomorrow! Tomorrow! I love ya Tomorrow! You're always a day away Tomorrow! Tomorrow! I love ya, tomorrow You're always a day away!!!!! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kallye Walther Sent: Tuesday, October 24, 2006 5:09 PM To: Barry.R.Rittman@uth.tmc.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] End Goodbye comments Well said Barry. For two days now I have seen a lot of emails on this hot topic. What I haven't seen is any more responses from Joe. I guess Joe has resovled the issue. It would be nice if the rest of us could do the same. Here's my thought, ( had to get it there ), The histonet was created for histology related issues, by allowing vendors to comment does benefit us. If a particular vendor has ABUSED this priviledge, then that is a matter for Linda M. and staff. By not allowing vendors to comment there WILL BE ISSUES that will be missed. I am NOT a vendor, but I deal with them daily as I am sure all of us do. Passwords are redundant, not just anyone visits Histonet, only histology related personel. The Histonet is a very valuable TOOL for histology. It does allow all of us to ask questions about issues that we obviously need advice on. It allows all of us to benefit from the experience of another. Of all the histology people subscribing on the Histonet, pooled that experience is probably hundreds of years. There is always something new out there. Without the freedom of uncensored speech, would this country even be here??? Isn;t that part of the reason we left that little island ( England ) a feww hundred years ago? Histonet allows all of us to benefit. Now I don't believe it should be used as a personal attack on anyone tech or vendor, but I sure as H--- don't think it should be turned into a political forum for tech vs. vendor either. This particular vendor should not have gone to Joe's boss, and in return Joe's boss, should not have repremanded him either. Why? other than the fact it is just wrong, how about.... the kick back law??? meaning, if Joe's boss would not stand behind Joe, and took sides of a vendor, the officiating powers that be that regulate kick backs, can certainly ask a few questions as to why???? This might be a bit of a stretch here...... but so far, so has the rest of this particular forum. I t is easy to see the outrage, as a fellow tech and supervisor, I AM outraged!!!! But since we histotechs stick together and will support each other, isn't it possible to think that there are 2 sides to every story, as well as pictures being painted in one's own color??? We all know Joe to be level headed, and distribute honest and sincere advise, so naturally we are all outraged. And while the rest of us are screaming foul.... Joe is certainly appreciating the support, however is would appear that this issue has been resolved with him,....... not here though. Just one tech's opinion. Thank you all for the comments, perspectives, and advice on this matter. Again this is what the Histonet WAS created for. Right Linda M????? >From: "Rittman, Barry R" >To: >Subject: [Histonet] End Goodbye comments >Date: Tue, 24 Oct 2006 14:22:18 -0500 > >There will never be an ideal solution, lets all use a little common >sense and tolerance for both vendors and customers and look at each >situation individually as one size solution does not fit all. > >Sometimes both vendors and customers are hoist by their own petard. > > > >It appears to me that it is now time for us to start discussing >histology topics again. > >Barry > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Stay in touch with old friends and meet new ones with Windows Live Spaces http://clk.atdmt.com/MSN/go/msnnkwsp0070000001msn/direct/01/?href=http://spa ces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From henriks <@t> southbaytech.com Tue Oct 24 16:50:10 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 16:52:24 2006 Subject: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Message-ID: <453E8A92.9010306@southbaytech.com> Douglas: How am I "grasping at straws"? The thing about the demo equipment is irrelevant. Geoff stated that I sent him "a very nasty off list reply" to a 3 year old message without providing any detail. My point was that Geoff's characterization of me and my message was absolutely, 100% incorrect. Geoff has made my point about posting something negative like that on the listserver. Some people will believe it is fact without knowing the whole story. The free exchange of scientific information is an appropriate use for the listserver. Using it as a weapon to attack someone you don't like or to harm a company you are unhappy with is not. Maybe I am idealistic, but it certainly seems reasonable to me. David Douglas D Deltour wrote: >It seems like you are grasping at straws again. I am sure Geoff is not lying >in bed at night stewing over a post three years ago. All he said is that he >wouldn't buy a piece of equipment without a demo. He voiced his opinion. He >is not a "disgruntled and frustrated poster". It is time to move on. > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 4:34 PM >To: Geoff McAuliffe >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >Dear List: > >I now understand why Geoff was so offended by my harmless comments. >This also makes my point as to why it is so important NOT to post your >negative opinions about a company - or an individual - on the >listserver. You can read the messages below which were retrieved from >the Microscopy listserver archives. My message to Geoff was not >"off-list" it was sent to him and the listserver. I'm confident that >once you read my message to him you will see that it was not nasty at >all. There is always more to the story than the single line some >disgruntled and frustrated poster may choose to share with you. Geoff >has obviously been stewing over my message for more than 3 years! >Geoff's own reference makes my case for me. > >This was posted on the Microscopy Listserver: > >From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz >Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) >Subject: LM - question on inverted microscope, Hoffman and VAREL contrast >Dear users of ListServer, > >I kindly ask you for your advice concerning inverted microscope. We need >to ....... > >Therefore, we consider to purchase an inverted microscope but we are not >sure if to ask for that one which is equipped with relief contrasting >method. If yes, which would be the best? Hoffman modulation ...... > >As we do not have a possibility to try inverted microscope with relief >contrast, I would very appreciate your opinion/advice. > >Thank you. > >Rostislav Zemek >Czech Republic E-mail: rosta-at-acarus.entu.cas.cz > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Tue, 04 Mar 2003 11:58:32 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Ask the vendor to bring the instrument you are considering to your lab for a >demonstration. We always do this for expensive items. Any vendor who >will not >comply is not worth dealing with. > >Geoff > >From: David Henriks : henriks-at-southbaytech.com >Date: Tue, 04 Mar 2003 18:26:26 -0800 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast >Geoff: > >I can appreciate that anyone would prefer to have a vendor deliver an >instrument to their door to do an on-site demonstration, but you also >must realize that it is simply not possible in every instance. To say >that "Any vendor who will not comply is not worth dealing with" is quite >simplistic and offensive. I have nothing to do with this particular >customer, but I can certainly understand how several excellent >microscopy vendors may have difficulty justifying an on-site demo in the >Czech Republic. I personally have been to the Czech Republic several >times to demonstrate our systems, however, it can certainly not be >justified in every instance. To dismiss vendors so capriciously would >be doing a disservice to Dr. Zemek. Perhaps the company offering the >best solution at the best price has a better method of demonstrating its >performance than through an on-site demo. Technology these days is >pretty impressive. > >Speaking as a family business owner and microscopy vendor for nearly 40 >years, I guess I take it a bit personally when vendors are treated with >such disrespect. Scientists and vendors working together rather than as >adversaries is much more productive and serves the scientific community >much more effectively. > >Best regards- > >David > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Wed, 05 Mar 2003 09:56:45 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Hi David: > >David Henriks wrote: > >} Geoff: >} >} I can appreciate that anyone would prefer to have a vendor deliver an >} instrument to their door to do an on-site demonstration, but you also >} must realize that it is simply not possible in every instance. > >That may be true. > >} To say >} that "Any vendor who will not comply is not worth dealing with" is quite >} simplistic and offensive. > >Hey, times are very tight here in University research land. We spend our >money the >way we want to. We can't afford to buy equipment we have not tested. If >this offends >you, so be it. In 30 years in university science, I have never had a >vendor refuse to >demo a product, even an inexpensive one. > >} I have nothing to do with this particular >} customer, but I can certainly understand how several excellent >} microscopy vendors may have difficulty justifying an on-site demo in the >} Czech Republic. > >If that is the nature of their business, then that is the nature of their >business. The vendor runs his business the way he wants to, the customer >spends his >money the way he wants to. Capitalism. > >} I personally have been to the Czech Republic several >} times to demonstrate our systems, however, it can certainly not be >} justified in every instance. To dismiss vendors so capriciously would >} be doing a disservice to Dr. Zemek. > >No, Dr Zemek can make his own decisions. I was suggesting one course of >action for his >consideration. > >} Perhaps the company offering the >} best solution at the best price has a better method of demonstrating its >} performance than through an on-site demo. Technology these days is >} pretty impressive.Speaking as a family business owner and microscopy >vendor for >} nearly 40 > >} years, I guess I take it a bit personally when vendors are treated with >} such disrespect. > >Not disrespect, it is just the way things operate in my world. How is >'not buying >a product without a demo to see if it fits your lab's needs' disrespectful?? > >} Scientists and vendors working together rather than as >} adversaries is much more productive and serves the scientific community >} much more effectively. > >That is a very nice, general statement that no one would argue with. It >is also >180 degrees opposite of buying a product without knowing if it will >solve your >problem. > >Geoff > > > > >Geoff McAuliffe wrote: > > > >>Dear list: >> >> David Henriks wrote a very nice-sounding reply to the vendor issue >>we are all concerned about. I say nice-sounding because while it may >>be the ideal situation, it is not always a real world situation. Joe's >>troubles bear this out. >> Several years ago someone posted a querry on this listserver (or >>the Microscopy listserver, I am not sure which) about purchasing an >>expensive piece of equipment. I suggested he invite vendors to bring a >>demo to his lab to see if their equipment fit his needs. I also >>suggested that any vendor who would not supply a demo not be >>considered in his purchase plans. I got a very nasty off list reply >>from .......... David Henriks of South Bay Technologies. >> Actions speak louder than words. >> >>Geoff >> >>David Henriks wrote: >> >> >> >>>I missed the earliest part of the thread here, but from what I see it >>>seems that someone criticized a vendor on the Histonet and then was >>>reprimanded by his boss for doing so after the vendor complained. >>>Now, everyone wants to ban vendors from the Histonet. I am a vendor >>>and I belong to many listservers. End users seem to have a very >>>skewed view of what a vendor is and what motivates us. My main >>>business is not histology, although we do offer some products for the >>>industry. Yes, companies are in business to make money. No >>>question. To say that patient care is not even on their radar screen >>>is ridiculous. If the customer's need is to improve patient care, >>>then that is what a vendor is striving for. Vendors make money by >>>supplying what customers want. If you want tools for better patient >>>care, then that is what we provide. If your needs change, then our >>>products change. We respond to your needs. If, as you say, patient >>>care is not even on the vendor radar screen, then that probably means >>>that customers have not made that a high priority in what they say >>>they need. Vendors do not make money by producing ineffective >>>products that customers don't want. The best way to ensure that >>>customers get what they need and vendors produce products to meet >>>those needs is to have an open forum where these issues can be freely >>>discussed. That requires interchange between end users and vendors. >>>Yes, there should be limitations - vendors should not overtly market >>>their products on the Histonet. They should respond to postings with >>>viable solutions - even if they involve their own products. Vendors >>>should not use this as a forum to denigrate their competition. By >>>the same token, end users should not use this as a forum to air their >>>grievances about a product or a manufacturer. A single negative >>>comment about a vendor or his product could have a devastating effect >>>on their business. Perhaps your complaints are justified, but there >>>are better ways to go about having your concerns heard. Any credible >>>vendor would love to hear from you if you are having a problem with >>>one of their products. Unfortunately, most customers do not contact >>>the company. Sometimes when they do contact a company they reach the >>>wrong person and do not get a satisfactory response. I can >>>understand the frustration. However, sharing your frustration in >>>such a public forum is not right. Just as it would not be right for >>>you to criticize one of your colleagues on the Histonet. >>>There are always the few that think vendors are their enemies and >>>think that they are solely motivated by greed. The reality is that >>>vendors and end users are in this together. The people that figure >>>that out early on are generally the most successful and the one who >>>actually drive the development of new products, new techniques and >>>make significant strides in their field. To ban vendors from your >>>site would be regrettable and counterproductive. >>> >>>The listserver should be used for a free exchange of ideas and not as >>>a venue for criticism. Praise in public, criticize in private. >>> >>>Can't we all just get along? >>> >>>Best regards- >>> >>>David >>> >>> >>> >> >> > > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. From Barry.R.Rittman <@t> uth.tmc.edu Tue Oct 24 17:00:40 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Oct 24 17:00:49 2006 Subject: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Message-ID: I would recommend that you use pistols at dawn and keep this between you two. I volunteer to bury the poorest shot. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 4:50 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Douglas: How am I "grasping at straws"? The thing about the demo equipment is irrelevant. Geoff stated that I sent him "a very nasty off list reply" to a 3 year old message without providing any detail. My point was that Geoff's characterization of me and my message was absolutely, 100% incorrect. Geoff has made my point about posting something negative like that on the listserver. Some people will believe it is fact without knowing the whole story. The free exchange of scientific information is an appropriate use for the listserver. Using it as a weapon to attack someone you don't like or to harm a company you are unhappy with is not. Maybe I am idealistic, but it certainly seems reasonable to me. David Douglas D Deltour wrote: >It seems like you are grasping at straws again. I am sure Geoff is not lying >in bed at night stewing over a post three years ago. All he said is that he >wouldn't buy a piece of equipment without a demo. He voiced his opinion. He >is not a "disgruntled and frustrated poster". It is time to move on. > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 4:34 PM >To: Geoff McAuliffe >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >Dear List: > >I now understand why Geoff was so offended by my harmless comments. >This also makes my point as to why it is so important NOT to post your >negative opinions about a company - or an individual - on the >listserver. You can read the messages below which were retrieved from >the Microscopy listserver archives. My message to Geoff was not >"off-list" it was sent to him and the listserver. I'm confident that >once you read my message to him you will see that it was not nasty at >all. There is always more to the story than the single line some >disgruntled and frustrated poster may choose to share with you. Geoff >has obviously been stewing over my message for more than 3 years! >Geoff's own reference makes my case for me. > >This was posted on the Microscopy Listserver: > >From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz >Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) >Subject: LM - question on inverted microscope, Hoffman and VAREL contrast >Dear users of ListServer, > >I kindly ask you for your advice concerning inverted microscope. We need >to ....... > >Therefore, we consider to purchase an inverted microscope but we are not >sure if to ask for that one which is equipped with relief contrasting >method. If yes, which would be the best? Hoffman modulation ...... > >As we do not have a possibility to try inverted microscope with relief >contrast, I would very appreciate your opinion/advice. > >Thank you. > >Rostislav Zemek >Czech Republic E-mail: rosta-at-acarus.entu.cas.cz > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Tue, 04 Mar 2003 11:58:32 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Ask the vendor to bring the instrument you are considering to your lab for a >demonstration. We always do this for expensive items. Any vendor who >will not >comply is not worth dealing with. > >Geoff > >From: David Henriks : henriks-at-southbaytech.com >Date: Tue, 04 Mar 2003 18:26:26 -0800 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast >Geoff: > >I can appreciate that anyone would prefer to have a vendor deliver an >instrument to their door to do an on-site demonstration, but you also >must realize that it is simply not possible in every instance. To say >that "Any vendor who will not comply is not worth dealing with" is quite >simplistic and offensive. I have nothing to do with this particular >customer, but I can certainly understand how several excellent >microscopy vendors may have difficulty justifying an on-site demo in the >Czech Republic. I personally have been to the Czech Republic several >times to demonstrate our systems, however, it can certainly not be >justified in every instance. To dismiss vendors so capriciously would >be doing a disservice to Dr. Zemek. Perhaps the company offering the >best solution at the best price has a better method of demonstrating its >performance than through an on-site demo. Technology these days is >pretty impressive. > >Speaking as a family business owner and microscopy vendor for nearly 40 >years, I guess I take it a bit personally when vendors are treated with >such disrespect. Scientists and vendors working together rather than as >adversaries is much more productive and serves the scientific community >much more effectively. > >Best regards- > >David > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Wed, 05 Mar 2003 09:56:45 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Hi David: > >David Henriks wrote: > >} Geoff: >} >} I can appreciate that anyone would prefer to have a vendor deliver an >} instrument to their door to do an on-site demonstration, but you also >} must realize that it is simply not possible in every instance. > >That may be true. > >} To say >} that "Any vendor who will not comply is not worth dealing with" is quite >} simplistic and offensive. > >Hey, times are very tight here in University research land. We spend our >money the >way we want to. We can't afford to buy equipment we have not tested. If >this offends >you, so be it. In 30 years in university science, I have never had a >vendor refuse to >demo a product, even an inexpensive one. > >} I have nothing to do with this particular >} customer, but I can certainly understand how several excellent >} microscopy vendors may have difficulty justifying an on-site demo in the >} Czech Republic. > >If that is the nature of their business, then that is the nature of their >business. The vendor runs his business the way he wants to, the customer >spends his >money the way he wants to. Capitalism. > >} I personally have been to the Czech Republic several >} times to demonstrate our systems, however, it can certainly not be >} justified in every instance. To dismiss vendors so capriciously would >} be doing a disservice to Dr. Zemek. > >No, Dr Zemek can make his own decisions. I was suggesting one course of >action for his >consideration. > >} Perhaps the company offering the >} best solution at the best price has a better method of demonstrating its >} performance than through an on-site demo. Technology these days is >} pretty impressive.Speaking as a family business owner and microscopy >vendor for >} nearly 40 > >} years, I guess I take it a bit personally when vendors are treated with >} such disrespect. > >Not disrespect, it is just the way things operate in my world. How is >'not buying >a product without a demo to see if it fits your lab's needs' disrespectful?? > >} Scientists and vendors working together rather than as >} adversaries is much more productive and serves the scientific community >} much more effectively. > >That is a very nice, general statement that no one would argue with. It >is also >180 degrees opposite of buying a product without knowing if it will >solve your >problem. > >Geoff > > > > >Geoff McAuliffe wrote: > > > >>Dear list: >> >> David Henriks wrote a very nice-sounding reply to the vendor issue >>we are all concerned about. I say nice-sounding because while it may >>be the ideal situation, it is not always a real world situation. Joe's >>troubles bear this out. >> Several years ago someone posted a querry on this listserver (or >>the Microscopy listserver, I am not sure which) about purchasing an >>expensive piece of equipment. I suggested he invite vendors to bring a >>demo to his lab to see if their equipment fit his needs. I also >>suggested that any vendor who would not supply a demo not be >>considered in his purchase plans. I got a very nasty off list reply >>from .......... David Henriks of South Bay Technologies. >> Actions speak louder than words. >> >>Geoff >> >>David Henriks wrote: >> >> >> >>>I missed the earliest part of the thread here, but from what I see it >>>seems that someone criticized a vendor on the Histonet and then was >>>reprimanded by his boss for doing so after the vendor complained. >>>Now, everyone wants to ban vendors from the Histonet. I am a vendor >>>and I belong to many listservers. End users seem to have a very >>>skewed view of what a vendor is and what motivates us. My main >>>business is not histology, although we do offer some products for the >>>industry. Yes, companies are in business to make money. No >>>question. To say that patient care is not even on their radar screen >>>is ridiculous. If the customer's need is to improve patient care, >>>then that is what a vendor is striving for. Vendors make money by >>>supplying what customers want. If you want tools for better patient >>>care, then that is what we provide. If your needs change, then our >>>products change. We respond to your needs. If, as you say, patient >>>care is not even on the vendor radar screen, then that probably means >>>that customers have not made that a high priority in what they say >>>they need. Vendors do not make money by producing ineffective >>>products that customers don't want. The best way to ensure that >>>customers get what they need and vendors produce products to meet >>>those needs is to have an open forum where these issues can be freely >>>discussed. That requires interchange between end users and vendors. >>>Yes, there should be limitations - vendors should not overtly market >>>their products on the Histonet. They should respond to postings with >>>viable solutions - even if they involve their own products. Vendors >>>should not use this as a forum to denigrate their competition. By >>>the same token, end users should not use this as a forum to air their >>>grievances about a product or a manufacturer. A single negative >>>comment about a vendor or his product could have a devastating effect >>>on their business. Perhaps your complaints are justified, but there >>>are better ways to go about having your concerns heard. Any credible >>>vendor would love to hear from you if you are having a problem with >>>one of their products. Unfortunately, most customers do not contact >>>the company. Sometimes when they do contact a company they reach the >>>wrong person and do not get a satisfactory response. I can >>>understand the frustration. However, sharing your frustration in >>>such a public forum is not right. Just as it would not be right for >>>you to criticize one of your colleagues on the Histonet. >>>There are always the few that think vendors are their enemies and >>>think that they are solely motivated by greed. The reality is that >>>vendors and end users are in this together. The people that figure >>>that out early on are generally the most successful and the one who >>>actually drive the development of new products, new techniques and >>>make significant strides in their field. To ban vendors from your >>>site would be regrettable and counterproductive. >>> >>>The listserver should be used for a free exchange of ideas and not as >>>a venue for criticism. Praise in public, criticize in private. >>> >>>Can't we all just get along? >>> >>>Best regards- >>> >>>David >>> >>> >>> >> >> > > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Oct 24 17:05:58 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Oct 24 17:07:08 2006 Subject: [Histonet]advice to vendors References: <453E5CF5.8080105@southbaytech.com> <453E69BE.1000609@umdnj.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A65F@fhosxchmb006.ADVENTISTCORP.NET> BETTER YET......The customer is always right!!!!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Geoff McAuliffe Sent: Tue 10/24/2006 3:30 PM To: David Henriks Cc: Douglas D Deltour; histonet@lists.utsouthwestern.edu; 'Monfils, Paul' Subject: Re: [Histonet]advice to vendors Vendors might want to remember, and practice, this old chestnut ......... "You never win an argument with a customer" Geoff David Henriks wrote: > Paul, Mark and Doug: > > I said nothing about censorship and I did not discourage anyone from > sharing their opinion. I suggested that those opinions be shared > off-line. Often times there is much more to the story than the > initial complaint might indicate. Getting the whole story out would > probably involve multiple emails and discussion. A recurrent theme I > am hearing is that if the vendor makes a good product and offers good > service, then they have nothing to worry about. I don't mean to be > flippant, but that is naive. Marketing is the name of the game. > Companies with great products and services go under all the time > because of negative publicity. Alternatively, companies with marginal > products and service often thrive due to extensive and aggressive > marketing. In this industry - as in many industries - the products > are produced by a large group of small companies. Many companies > don't have a huge marketing budget that would allow them to counteract > the negative publicity given on a site like this. How is a vendor to > respond? Do you want the vendor to continue a thread on the Histonet > providing the details of the individual incident? Is that > appropriate? Or, does the vendor ignore it and leave that negative > impression hanging in the air to the countless members that have seen > it and tucked away that thought in the back of their minds. Or, as > has been suggested, do you ban vendors altogether so there is no forum > at all for them to respond. > > As for Doug's comment about making a positive comment, of course I > wouldn't mind that. That's why I said "praise in public, criticize in > private". There is no harm to a vendor by publicly praising them. > Just as there is no harm to the inquirer if they receive criticism > about the vendor they are considering in private. > > I'm sure you wouldn't have a problem if your boss posted to the > Histonet that you are the greatest histologist in the history of > histology. Would you have a problem if he posted to the Histonet that > he had some problems with you and that he questioned your ability to > perform your job? If you're a great histologist, I'm sure that it > wouldn't bother you to have negative comments posted about you for > everyone to see. After all, your work should speak for itself. Maybe > some people had never heard of you and were not aware that you are the > worlds greatest histologist. Do you think their opinion of you might > be skewed a little bit by the negative comment? > > We are all "vendors" to some degree. We are either selling our > talents to our employer or selling our goods to an end user. We are > all an integral and equal part of the industry and it is wrong to > treat vendors as if they are anything less. > > Best regards- > > David > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Janet.Bonner <@t> FLHOSP.ORG Tue Oct 24 17:27:48 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Oct 24 17:28:50 2006 Subject: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it References: Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A662@fhosxchmb006.ADVENTISTCORP.NET> This leaves nothing for Friday, we'll be all worn out! :( ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rittman, Barry R Sent: Tue 10/24/2006 6:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it I would recommend that you use pistols at dawn and keep this between you two. I volunteer to bury the poorest shot. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 4:50 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Douglas: How am I "grasping at straws"? The thing about the demo equipment is irrelevant. Geoff stated that I sent him "a very nasty off list reply" to a 3 year old message without providing any detail. My point was that Geoff's characterization of me and my message was absolutely, 100% incorrect. Geoff has made my point about posting something negative like that on the listserver. Some people will believe it is fact without knowing the whole story. The free exchange of scientific information is an appropriate use for the listserver. Using it as a weapon to attack someone you don't like or to harm a company you are unhappy with is not. Maybe I am idealistic, but it certainly seems reasonable to me. David Douglas D Deltour wrote: >It seems like you are grasping at straws again. I am sure Geoff is not lying >in bed at night stewing over a post three years ago. All he said is that he >wouldn't buy a piece of equipment without a demo. He voiced his opinion. He >is not a "disgruntled and frustrated poster". It is time to move on. > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 4:34 PM >To: Geoff McAuliffe >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >Dear List: > >I now understand why Geoff was so offended by my harmless comments. >This also makes my point as to why it is so important NOT to post your >negative opinions about a company - or an individual - on the >listserver. You can read the messages below which were retrieved from >the Microscopy listserver archives. My message to Geoff was not >"off-list" it was sent to him and the listserver. I'm confident that >once you read my message to him you will see that it was not nasty at >all. There is always more to the story than the single line some >disgruntled and frustrated poster may choose to share with you. Geoff >has obviously been stewing over my message for more than 3 years! >Geoff's own reference makes my case for me. > >This was posted on the Microscopy Listserver: > >From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz >Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) >Subject: LM - question on inverted microscope, Hoffman and VAREL contrast >Dear users of ListServer, > >I kindly ask you for your advice concerning inverted microscope. We need >to ....... > >Therefore, we consider to purchase an inverted microscope but we are not >sure if to ask for that one which is equipped with relief contrasting >method. If yes, which would be the best? Hoffman modulation ...... > >As we do not have a possibility to try inverted microscope with relief >contrast, I would very appreciate your opinion/advice. > >Thank you. > >Rostislav Zemek >Czech Republic E-mail: rosta-at-acarus.entu.cas.cz > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Tue, 04 Mar 2003 11:58:32 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Ask the vendor to bring the instrument you are considering to your lab for a >demonstration. We always do this for expensive items. Any vendor who >will not >comply is not worth dealing with. > >Geoff > >From: David Henriks : henriks-at-southbaytech.com >Date: Tue, 04 Mar 2003 18:26:26 -0800 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast >Geoff: > >I can appreciate that anyone would prefer to have a vendor deliver an >instrument to their door to do an on-site demonstration, but you also >must realize that it is simply not possible in every instance. To say >that "Any vendor who will not comply is not worth dealing with" is quite >simplistic and offensive. I have nothing to do with this particular >customer, but I can certainly understand how several excellent >microscopy vendors may have difficulty justifying an on-site demo in the >Czech Republic. I personally have been to the Czech Republic several >times to demonstrate our systems, however, it can certainly not be >justified in every instance. To dismiss vendors so capriciously would >be doing a disservice to Dr. Zemek. Perhaps the company offering the >best solution at the best price has a better method of demonstrating its >performance than through an on-site demo. Technology these days is >pretty impressive. > >Speaking as a family business owner and microscopy vendor for nearly 40 >years, I guess I take it a bit personally when vendors are treated with >such disrespect. Scientists and vendors working together rather than as >adversaries is much more productive and serves the scientific community >much more effectively. > >Best regards- > >David > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Wed, 05 Mar 2003 09:56:45 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Hi David: > >David Henriks wrote: > >} Geoff: >} >} I can appreciate that anyone would prefer to have a vendor deliver an >} instrument to their door to do an on-site demonstration, but you also >} must realize that it is simply not possible in every instance. > >That may be true. > >} To say >} that "Any vendor who will not comply is not worth dealing with" is quite >} simplistic and offensive. > >Hey, times are very tight here in University research land. We spend our >money the >way we want to. We can't afford to buy equipment we have not tested. If >this offends >you, so be it. In 30 years in university science, I have never had a >vendor refuse to >demo a product, even an inexpensive one. > >} I have nothing to do with this particular >} customer, but I can certainly understand how several excellent >} microscopy vendors may have difficulty justifying an on-site demo in the >} Czech Republic. > >If that is the nature of their business, then that is the nature of their >business. The vendor runs his business the way he wants to, the customer >spends his >money the way he wants to. Capitalism. > >} I personally have been to the Czech Republic several >} times to demonstrate our systems, however, it can certainly not be >} justified in every instance. To dismiss vendors so capriciously would >} be doing a disservice to Dr. Zemek. > >No, Dr Zemek can make his own decisions. I was suggesting one course of >action for his >consideration. > >} Perhaps the company offering the >} best solution at the best price has a better method of demonstrating its >} performance than through an on-site demo. Technology these days is >} pretty impressive.Speaking as a family business owner and microscopy >vendor for >} nearly 40 > >} years, I guess I take it a bit personally when vendors are treated with >} such disrespect. > >Not disrespect, it is just the way things operate in my world. How is >'not buying >a product without a demo to see if it fits your lab's needs' disrespectful?? > >} Scientists and vendors working together rather than as >} adversaries is much more productive and serves the scientific community >} much more effectively. > >That is a very nice, general statement that no one would argue with. It >is also >180 degrees opposite of buying a product without knowing if it will >solve your >problem. > >Geoff > > > > >Geoff McAuliffe wrote: > > > >>Dear list: >> >> David Henriks wrote a very nice-sounding reply to the vendor issue >>we are all concerned about. I say nice-sounding because while it may >>be the ideal situation, it is not always a real world situation. Joe's >>troubles bear this out. >> Several years ago someone posted a querry on this listserver (or >>the Microscopy listserver, I am not sure which) about purchasing an >>expensive piece of equipment. I suggested he invite vendors to bring a >>demo to his lab to see if their equipment fit his needs. I also >>suggested that any vendor who would not supply a demo not be >>considered in his purchase plans. I got a very nasty off list reply >>from .......... David Henriks of South Bay Technologies. >> Actions speak louder than words. >> >>Geoff >> >>David Henriks wrote: >> >> >> >>>I missed the earliest part of the thread here, but from what I see it >>>seems that someone criticized a vendor on the Histonet and then was >>>reprimanded by his boss for doing so after the vendor complained. >>>Now, everyone wants to ban vendors from the Histonet. I am a vendor >>>and I belong to many listservers. End users seem to have a very >>>skewed view of what a vendor is and what motivates us. My main >>>business is not histology, although we do offer some products for the >>>industry. Yes, companies are in business to make money. No >>>question. To say that patient care is not even on their radar screen >>>is ridiculous. If the customer's need is to improve patient care, >>>then that is what a vendor is striving for. Vendors make money by >>>supplying what customers want. If you want tools for better patient >>>care, then that is what we provide. If your needs change, then our >>>products change. We respond to your needs. If, as you say, patient >>>care is not even on the vendor radar screen, then that probably means >>>that customers have not made that a high priority in what they say >>>they need. Vendors do not make money by producing ineffective >>>products that customers don't want. The best way to ensure that >>>customers get what they need and vendors produce products to meet >>>those needs is to have an open forum where these issues can be freely >>>discussed. That requires interchange between end users and vendors. >>>Yes, there should be limitations - vendors should not overtly market >>>their products on the Histonet. They should respond to postings with >>>viable solutions - even if they involve their own products. Vendors >>>should not use this as a forum to denigrate their competition. By >>>the same token, end users should not use this as a forum to air their >>>grievances about a product or a manufacturer. A single negative >>>comment about a vendor or his product could have a devastating effect >>>on their business. Perhaps your complaints are justified, but there >>>are better ways to go about having your concerns heard. Any credible >>>vendor would love to hear from you if you are having a problem with >>>one of their products. Unfortunately, most customers do not contact >>>the company. Sometimes when they do contact a company they reach the >>>wrong person and do not get a satisfactory response. I can >>>understand the frustration. However, sharing your frustration in >>>such a public forum is not right. Just as it would not be right for >>>you to criticize one of your colleagues on the Histonet. >>>There are always the few that think vendors are their enemies and >>>think that they are solely motivated by greed. The reality is that >>>vendors and end users are in this together. The people that figure >>>that out early on are generally the most successful and the one who >>>actually drive the development of new products, new techniques and >>>make significant strides in their field. To ban vendors from your >>>site would be regrettable and counterproductive. >>> >>>The listserver should be used for a free exchange of ideas and not as >>>a venue for criticism. Praise in public, criticize in private. >>> >>>Can't we all just get along? >>> >>>Best regards- >>> >>>David >>> >>> >>> >> >> > > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From AnthonyH <@t> chw.edu.au Tue Oct 24 17:46:23 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Oct 24 17:48:02 2006 Subject: [Histonet] Formalin storage Message-ID: Tom, Unfortunately this may be the way things go, though the infectious risk must be considered. We have a policy for the clean-up of formalin spills and a spills kit near our 10l container of 10%NBF that is used in theatres. We also have clear approved labelling of the 10%NBF container. We have started adding the indicator dye Bromocresol purple to highlight the solution as formalin. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, 25 October 2006 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin storage I have a meeting tomorrow to discuss the keeping of formalin in the surgery and L&D areas. The hospital just had a JCAHO inspection and the inspectors didn't like it very much. They suggested that the 5 gallon carboys should be stored in hoods or removed from the areas. They went on to say that all specimens should be taken to Histology as fresh specimens and that the Histology personnel should be the ones putting formalin on the specimens. The inspectors said that that was how all of the larger hospitals did it but no one that I have spoken to does it that way. So my question.... Does anybody (or a significant number of you) do it this way? Personally, I can't see how this can be good for the specimens. Specimens would have to be delivered immediately and someone would have to be available 24/7 to take care of them. I just don't see how it can work. I would appreciate any thoughts. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Oct 24 17:50:29 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Oct 24 17:52:10 2006 Subject: [Histonet] Re: goodbye Message-ID: I continually remind any vendors whose performance is sub-optimal that if I get sued by a family for incorrect results resulting from their products that my lawyer will be on their case before they have time to cough!! So far no disgruntled families!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, 25 October 2006 12:35 AM To: Andrea Grantham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye Because they are, essentially, in the business of making money; patient care is usually not even in their "radar screen"! Ren? J. Andrea Grantham wrote: It happened to me also - maybe this happens more than we think??? I was chastised by a vendor when I asked if anybody else was experiencing a certain phenomenon with one of their products. He told me that I should have gone to them instead of asking my question on histonet. Well, I did and really didn't get any help from them when I did this before going to histonet. Instead they were trying to tell me to change my technique. I don't know about you, but after you do something one way for 25 or more years it would seem that it wouldn't be the technique - or not usually. Long story short, we danced around this problem until somebody finally told me they had a bad batch of base "product" that went into certain lots. Bingo! Thank God for the brave soul that finally admitted that the company did have a problem but why couldn't they admit it weeks sooner and save me the grief that I had with my tissues? By the way, there were others out in histoland having the same problem. Hope they got it fixed too. Andi At 10:03 AM 10/23/2006 -0700, Erin.Wrona@kp.org wrote: >Amos wrote: "This happened to me before too. A vendor that will remain >nameless >(that almost everyone has some equipment sold to them by these people) >took >issue with my stating that we were having trouble with an expensive item >they sold us when someone asked about this item on the Histonet." > >I am sorry to say that this happened to me as well at a previous job. >Since then I am always VERY careful about what I say (not much after >the >incident) about any product. It's sad but sales reps seem to be rather >aggressive in acting to silence criticism of their (extremely expensive) >products. > >-Erin > >NOTICE TO RECIPIENT: If you are not the intended recipient of this >e-mail, you are prohibited >from sharing, copying, or otherwise using or disclosing its contents. If >you have received this e-mail in error, please notify the sender >immediately by reply e-mail and permanently delete this e-mail and any >attachments without reading, forwarding or saving them. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From amosbrooks <@t> gmail.com Tue Oct 24 19:04:21 2006 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Oct 24 19:04:28 2006 Subject: [Histonet] Vendor Perspective Message-ID: <582736990610241704p2197e82cwcc89ed369612d404@mail.gmail.com> David, I have to respectfully disagree. This is a group forum, and while I feel stifled at times about offering my experiences with products, it would entirely defeat the purpose of this forum if only the sunshiny glowing references were permitted. It's just not a reflection of reality. Not everyone will like "Product A" and they have every right to say so. If you feel there is any basis to fight this First Ammendment case I think you should start with the bumperstickers of car manufacturer logos being watered down. (see: http://www.customnames.com/images/calvin_Ford.jpg ) (Apologies to Ford Royer, tough thread my friend) If you can get anywhere with that then I'm sure you will see people being more cautious about saying anything bad about your products. They will probably all be moving to North Korea for more personal freedom too. The ideal thing for the vendor to do would be to contact the person making the complaint and see what can be done to fix the problem. Accepting the fact that it is entirely possible that the customer may be too far gone to do anything about. No harm in trying though. Now it must be said that there is a limit to expressing a negative opinion and simply slandreing or bashing a company. This is unacceptable too and in this case the vendor needs to do what they need to do to stay alive. Honestly though one hot headed remark does not slander make. If it really bugs you find a customer you have a good relationship with to counter the claim. I have to disagree emphatically with any call to have vendors bumped off the Histonet. As a matter of fact I think we need more, but they need to understand the basics of the Histonet. I have had Vendors and Sales Reps give very good advice and have benefitted very much from their help over the years. But it is imperative that they keep in mind the purpose of this forum. It is not to be used as a sales platform. If you are helpful and answer customer's questions they will remember you. If you hunt them down and chastize them for daring to not like your product ... they will remember you. Cheers, Amos Brooks -------------------------------------------- From: David Henriks Subject: Re: [Histonet] Re: goodbye - vendor perspective To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Message-ID: <453E48AE.2050505@southbaytech.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed John should answer Jane's question. John should answer Jane's question directly to Jane. John should not post his comments to the listserver as a whole. -- David Henriks From henriks <@t> southbaytech.com Tue Oct 24 21:38:50 2006 From: henriks <@t> southbaytech.com (David Henriks) Date: Tue Oct 24 21:38:46 2006 Subject: [Histonet] Vendor Perspective In-Reply-To: <582736990610241704p2197e82cwcc89ed369612d404@mail.gmail.com> References: <582736990610241704p2197e82cwcc89ed369612d404@mail.gmail.com> Message-ID: <453ECE3A.1050203@southbaytech.com> Amos: Everyone seems to misunderstand what I said. I never said not to share your opinion. I said to share it in private. I would support stating your negative opinions on the listserver if the entire conversation took place on the listserver. I think it is unfair for someone to toss out a negative opinion without including the background and without the vendor having the opportunity to respond. I don't think anyone wants to be inundated with all of the details of someone's individual situation, however I think it is necessary to put the comments in context. As an example, one of the listserve members made some negative personal comments about me. When I defended myself and tried to provide the complete picture I was greeted with people telling me I was "grasping at straws" and told to "shut the *&?! up". With that in mind, it seems that a vendor won't get a fair shake here even to defend his good name. It seems to me that it makes more sense to send the negative comments off line and let the people who have the real interest sort it out. Alternatively, you need to happily accept the entire thread from anyone who wants to post without castigating them both on and off line. Also, it should be noted that one hot headed remark certainly can be slanderous - particularly when made openly to a forum of thousands of potential customers, employers and colleagues. Even within the first amendment there are limits to what can be said. Although, I don't see this as so much a 1st amendment issue as I do an issue of common decency. Best regards- David Amos Brooks wrote: > David, > I have to respectfully disagree. This is a group forum, and while I > feel stifled at times about offering my experiences with products, it > would > entirely defeat the purpose of this forum if only the sunshiny glowing > references were permitted. It's just not a reflection of reality. Not > everyone will like "Product A" and they have every right to say so. > If you feel there is any basis to fight this First Ammendment case I > think you should start with the bumperstickers of car manufacturer logos > being watered down. (see: > http://www.customnames.com/images/calvin_Ford.jpg ) > (Apologies to Ford Royer, tough thread my friend) If you can get anywhere > with that then I'm sure you will see people being more cautious about > saying > anything bad about your products. They will probably all be moving to > North > Korea for more personal freedom too. > The ideal thing for the vendor to do would be to contact the person > making the complaint and see what can be done to fix the problem. > Accepting > the fact that it is entirely possible that the customer may be too far > gone > to do anything about. No harm in trying though. > Now it must be said that there is a limit to expressing a negative > opinion and simply slandreing or bashing a company. This is > unacceptable too > and in this case the vendor needs to do what they need to do to stay > alive. > Honestly though one hot headed remark does not slander make. If it really > bugs you find a customer you have a good relationship with to counter the > claim. > I have to disagree emphatically with any call to have vendors bumped > off the Histonet. As a matter of fact I think we need more, but they > need to > understand the basics of the Histonet. I have had Vendors and Sales Reps > give very good advice and have benefitted very much from their help > over the > years. But it is imperative that they keep in mind the purpose of this > forum. It is not to be used as a sales platform. If you are helpful and > answer customer's questions they will remember you. If you hunt them down > and chastize them for daring to not like your product ... they will > remember > you. > > Cheers, > Amos Brooks > > -------------------------------------------- > From: David Henriks > Subject: Re: [Histonet] Re: goodbye - vendor perspective > To: Douglas D Deltour > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <453E48AE.2050505@southbaytech.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > John should answer Jane's question. > > John should answer Jane's question directly to Jane. > > John should not post his comments to the listserver as a whole. > > -- > David Henriks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: 949-492-2600 Toll-free in the USA: 800-728-2233 FAX: 949-492-1499 email: henriks+AEA-southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling 949-492-2600 and delete this message from your system. From suetp918 <@t> comcast.net Tue Oct 24 21:56:20 2006 From: suetp918 <@t> comcast.net (suetp918@comcast.net) Date: Tue Oct 24 21:56:28 2006 Subject: [Histonet] coverslipping film Message-ID: <102520060256.16755.453ED254000CEE1F000041732207022933C8CEC79F9B0A9A9C@comcast.net> I am new to the list so this is a little late. But we have devised a good way to handle the old archieved slides that have the slide separated from the coverslip. Most of the time the coverslip will curl and keeping it on the slide isl tough at best. Cut as much of the coverslip away from the tissue leaving just a small frame of coverslipping film around the section. Depending on the size of your cut place some mounting media on a slide as well as a little xylenel and that mount the seciton you have just cut. Good luck. -- Susan T. Paturzo HT (ASCP) Thomas Jefferson University Hospital Supervisor, Department of Pathology 132 S. 10th Street 263 Main Bldg. Phila., PA 19107 215-955-4179 215-923-1969 (FAX) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 25 02:45:54 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 25 02:45:07 2006 Subject: [Histonet] sections curling Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC826@wahtntex2.waht.swest.nhs.uk> Hello, sorry for the simplistic question. We are having a lot of problem with some sections curling and wrinkling. this is decalcified bone in parafin. We are using a sliding microtome with disposable blades. is it the blades? This is my first use of disposables. It seems like the answer is very simple, but I have drawn a blank. Bet it's the processing, but it could be the angle of the blade. Revisit your processing schedule and try altering the angle of attack for your blade (what is the correct name for that angle?). Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Nobody grows old merely by living a number of years. We grow old by deserting our ideals. Years may wrinkle the skin, but to give up enthusiasm wrinkles the soul. --Samuel Ullman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From mtyler <@t> uctgsh1.uct.ac.za Wed Oct 25 03:05:50 2006 From: mtyler <@t> uctgsh1.uct.ac.za (Tyler) Date: Wed Oct 25 04:01:31 2006 Subject: [Histonet] double staining Message-ID: <453F1ADE.91698D45@uctgsh1.uct.ac.za> Morning to All Histonetters Request from one of the Med Techs I work with. Please can someone forward or suggest a method for double staining on tissue sections using DAB and AEC substrate. Thanks Marilyn From cdemarinis <@t> SARATOGACARE.ORG Wed Oct 25 06:21:18 2006 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Wed Oct 25 06:21:27 2006 Subject: [Histonet] Anatomic Pathology Manager job description Message-ID: Anatomic Pathology Manager Would anyone be willing to share a job description for their position titled, manager, anatomic pathology? Thank you. From Lchausse <@t> nmh.org Wed Oct 25 07:19:23 2006 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Wed Oct 25 07:19:51 2006 Subject: [Histonet] Microwave processing for renal biopsies Message-ID: Does anyone have experience with microwave processing specific to STAT renal biopsies? Thanks. Leslie J Chaussey Manager, Surgical Pathology Northwestern Memorial Hospital 312 / 926-9510 lchausse@nmh.org ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From kgrobert <@t> rci.rutgers.edu Wed Oct 25 08:48:22 2006 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Oct 25 08:46:03 2006 Subject: [Histonet] Re: goodbye - vendor perspective-histotech opinion In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BA1@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E273BA1@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <453F6B26.7080207@rci.rutgers.edu> Hmmm. I guess that I consider Histonet a more specialized version of the more "everyday" bulletin boards and (sort of) online shopping sites like BizRate and those on internet portals like AOL and Yahoo!, etc. Every one of those sites has a forum for posting opinions and ratings about how stuff works or doesn't work, companies that supply/deliver/repair said products, etc. I would be surprised if the companies aren't actively monitoring these sites to get a handle on how their products are doing. Essentially it's marketing research for free, right? Why should Histonet be any different? Kathleen Rutgers University Monfils, Paul wrote: >David H., > >While I agree with much of what you said, I nevertheless fully support the >practice of seeking feedback/recommendations - positive or negative - about >an expensive piece of equipment before purchasing it. Obviously, other >users of the equipment are the only reliable source from which such >information can be obtained. Offering a response to such a query does not >constitute "airing grievances". It constitutes sharing of valuable >information which consumers have a right to know. I realize that any >company, even the best, can run into a problem now and then. But I don't see >why that should present a problem. If I request information from users about >a particular product or company, and one person responds negatively - which >he/she has every right to do - while a dozen others report satisfaction, >then of course I will go ahead and try the product or company myself. But if >a majority of people report problems with a product or company, then of >course I'll keep my distance. Companies which consistently provide good >products and good service only stand to gain from such online >recommendations. Companies which provide poor products and/or poor service >have made their own beds and will just have to lie in them. > > >Paul M. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From d.a.faichney <@t> stir.ac.uk Wed Oct 25 08:46:00 2006 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Wed Oct 25 08:46:27 2006 Subject: [Histonet] Repetitive strain? Message-ID: <38E5B431A5228D46A9A99E0206D5788317CE55@medwin.ad.stir.ac.uk> Hello all, I have worked within Histology for 18 years and have progressively felt muscle fatigue and discomfort in the wrists, elbows and neck. I also have numbness and tingling in my left index finger. It is now being addressed by my Occupation Health department (although they are mainly used to Display screen monitoring as we are in a university setting) In the meantime, I thought I may gain some ergonomics advice or alternative methods used by those experiencing the same or similar. I believe that it is mainly manual microtomy and forceps work which are causing the problems. I remember seeing something once about a tennis ball being placed over the handwheel to give a better gripping position. Anyone else know of this? Or a better solution? Also, another person mentioned using Scissor forceps, are these any good? Any and all responses welcomed. Debbie Faichney Institute of Aquaculture Histopathology University of Stirling Stirling Scotland UK -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 25 09:06:55 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 25 09:06:14 2006 Subject: [Histonet] Repetitive strain? Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC83D@wahtntex2.waht.swest.nhs.uk> I have worked within Histology for 18 years and have progressively felt muscle fatigue and discomfort in the wrists, elbows and neck. I also have numbness and tingling in my left index finger. It is now being addressed by my Occupation Health department (although they are mainly used to Display screen monitoring as we are in a university setting) In the meantime, I thought I may gain some ergonomics advice or alternative methods used by those experiencing the same or similar. I believe that it is mainly manual microtomy and forceps work which are causing the problems. I remember seeing something once about a tennis ball being placed over the handwheel to give a better gripping position. Anyone else know of this? Or a better solution? Also, another person mentioned using Scissor forceps, are these any good? Can you not use the motorised microtomes that are on the market? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jqb7 <@t> cdc.gov Wed Oct 25 08:57:23 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Oct 25 09:07:42 2006 Subject: [Histonet] Repetitive strain? Message-ID: Debbie, I have had surgery on both hands for carpal tunnel (successfully, thank goodness!) but do want to mention some items that can help with the problem. I use the Surgipath ergonomic forceps and they have helped a lot. Also, if you don't have the money for a completely automated microtome, the Microm Ergostar is a great alternative. It is a rotary microtome but instead of turning a wheel you push/pull a handle on either side. I loved this machine but love even better the fully automated one I now have. But the Ergostar is a great, relatively inexpensive alternative. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deborah Faichney Sent: Wednesday, October 25, 2006 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Repetitive strain? Hello all, I have worked within Histology for 18 years and have progressively felt muscle fatigue and discomfort in the wrists, elbows and neck. I also have numbness and tingling in my left index finger. It is now being addressed by my Occupation Health department (although they are mainly used to Display screen monitoring as we are in a university setting) In the meantime, I thought I may gain some ergonomics advice or alternative methods used by those experiencing the same or similar. I believe that it is mainly manual microtomy and forceps work which are causing the problems. I remember seeing something once about a tennis ball being placed over the handwheel to give a better gripping position. Anyone else know of this? Or a better solution? Also, another person mentioned using Scissor forceps, are these any good? Any and all responses welcomed. Debbie Faichney Institute of Aquaculture Histopathology University of Stirling Stirling Scotland UK -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 25 09:13:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 25 09:13:26 2006 Subject: [Histonet] double staining In-Reply-To: <453F1ADE.91698D45@uctgsh1.uct.ac.za> Message-ID: <20061025141313.66052.qmail@web61221.mail.yahoo.com> It is always preferable to double stain using DAB for one Ab and DAB+Nickel sulfate for the other Ab; the first will be stained brownish and the second bluish. AEC easily fades and is less permanent than DAB besides being similar in colour. Ren? J. Tyler wrote: Morning to All Histonetters Request from one of the Med Techs I work with. Please can someone forward or suggest a method for double staining on tissue sections using DAB and AEC substrate. Thanks Marilyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From Rae.Staskiewicz <@t> Illinois.gov Wed Oct 25 09:29:31 2006 From: Rae.Staskiewicz <@t> Illinois.gov (Staskiewicz, Rae) Date: Wed Oct 25 09:30:24 2006 Subject: [Histonet] Microbiology question Message-ID: I have a microbiologist here who has a question. Maybe some of you are close to the micro lab and can ask your biologist. What is a bijou bottle, and can you send a picture of one? Rae Ann Staskiewicz Galesburg Animal Disease Lab Galesburg, IL From TownsendD <@t> childrensdayton.org Wed Oct 25 09:40:54 2006 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Oct 25 09:41:58 2006 Subject: [Histonet] Microbiology question Message-ID: <20061025T104054Z_6258000F0000@childrensdayton.org> It's a small screw cap glass bottle as seen below. In the second picture, the bijou bottle is the smallest one, 7 ml Dolores From HornHV <@t> archildrens.org Wed Oct 25 09:59:34 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Oct 25 09:59:59 2006 Subject: [Histonet] lyve-1 staining Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6E4B@EMAIL.archildrens.org> I need to get this test done and I can't find anyone who does this. I have contacted ARUP, Mayo and Specialty Labs. Any ideas? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From ander093 <@t> tc.umn.edu Wed Oct 25 10:27:33 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Oct 25 10:27:51 2006 Subject: [Histonet] Vendor Perspective In-Reply-To: <582736990610241704p2197e82cwcc89ed369612d404@mail.gmail.co m> References: <582736990610241704p2197e82cwcc89ed369612d404@mail.gmail.com> Message-ID: <6.2.3.4.0.20061025102347.01db8c58@ander093.email.umn.edu> Very well said Amos. I don't think David is getting what we are trying to say. The information, opinions, etc. voiced on histonet are for everyone on histonet. Answering one person privately defeats the whole purpose. I agree that venders should contact the person with the complaint and not their bosses~~I believe that reputable venders would do just that. I agree also that we need to have the venders on the list. Theres the old saying about one bad apple....................... LuAnn At 07:04 PM 10/24/2006, Amos Brooks wrote: >David, > I have to respectfully disagree. This is a group forum, and while I >feel stifled at times about offering my experiences with products, it would >entirely defeat the purpose of this forum if only the sunshiny glowing >references were permitted. It's just not a reflection of reality. Not >everyone will like "Product A" and they have every right to say so. > If you feel there is any basis to fight this First Ammendment case I >think you should start with the bumperstickers of car manufacturer logos >being watered down. (see: http://www.customnames.com/images/calvin_Ford.jpg ) >(Apologies to Ford Royer, tough thread my friend) If you can get anywhere >with that then I'm sure you will see people being more cautious about saying >anything bad about your products. They will probably all be moving to North >Korea for more personal freedom too. > The ideal thing for the vendor to do would be to contact the person >making the complaint and see what can be done to fix the problem. Accepting >the fact that it is entirely possible that the customer may be too far gone >to do anything about. No harm in trying though. > Now it must be said that there is a limit to expressing a negative >opinion and simply slandreing or bashing a company. This is unacceptable too >and in this case the vendor needs to do what they need to do to stay alive. >Honestly though one hot headed remark does not slander make. If it really >bugs you find a customer you have a good relationship with to counter the >claim. > I have to disagree emphatically with any call to have vendors bumped >off the Histonet. As a matter of fact I think we need more, but they need to >understand the basics of the Histonet. I have had Vendors and Sales Reps >give very good advice and have benefitted very much from their help over the >years. But it is imperative that they keep in mind the purpose of this >forum. It is not to be used as a sales platform. If you are helpful and >answer customer's questions they will remember you. If you hunt them down >and chastize them for daring to not like your product ... they will remember >you. > >Cheers, >Amos Brooks > >-------------------------------------------- >From: David Henriks >Subject: Re: [Histonet] Re: goodbye - vendor perspective >To: Douglas D Deltour >Cc: histonet@lists.utsouthwestern.edu >Message-ID: <453E48AE.2050505@southbaytech.com> >Content-Type: text/plain; charset=ISO-8859-1; format=flowed > >John should answer Jane's question. > >John should answer Jane's question directly to Jane. > >John should not post his comments to the listserver as a whole. > >-- >David Henriks >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pantur <@t> aol.com Wed Oct 25 10:41:07 2006 From: Pantur <@t> aol.com (Pantur@aol.com) Date: Wed Oct 25 10:41:23 2006 Subject: [Histonet] Joe's problems - a different take Message-ID: I am the only one to suggest that the real villains here are Joe's supervisors that reprimanded him for expressing his opinion? Angelos Panagopoulos Manager Pantur, Inc. 5126 East 5th Street............P.O. Box 6471 Austin, TX 78702.................Austin, TX 78762 Phone: (512) 385-6232 Fax: (512) 385-6253 e-mail: pantur@aol.com From Terry.Marshall <@t> rothgen.nhs.uk Wed Oct 25 10:55:03 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Oct 25 10:57:59 2006 Subject: [Histonet] Joe's problems - a different take Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C47@LIL.xRothGen.nhs.uk> I don't agree. What do you think the person who grassed on him had in mind, to get him a promotion? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Pantur@aol.com [mailto:Pantur@aol.com] Sent: 25 October 2006 16:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Joe's problems - a different take I am the only one to suggest that the real villains here are Joe's supervisors that reprimanded him for expressing his opinion? Angelos Panagopoulos Manager Pantur, Inc. 5126 East 5th Street............P.O. Box 6471 Austin, TX 78702.................Austin, TX 78762 Phone: (512) 385-6232 Fax: (512) 385-6253 e-mail: pantur@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Oct 25 11:03:47 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Oct 25 10:59:55 2006 Subject: [Histonet] Joe's problems - a different take Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684B78@bruexchange.digestivespecialists.com> Shall we all collectively yell "JOE --- FIND A NEW JOB!" I agree with you. I wouldn't want to work for someone that wouldn't stand behind me. Did that once a long time ago, and won't do it again. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pantur@aol.com Sent: Wednesday, October 25, 2006 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Joe's problems - a different take I am the only one to suggest that the real villains here are Joe's supervisors that reprimanded him for expressing his opinion? Angelos Panagopoulos Manager Pantur, Inc. 5126 East 5th Street............P.O. Box 6471 Austin, TX 78702.................Austin, TX 78762 Phone: (512) 385-6232 Fax: (512) 385-6253 e-mail: pantur@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Oct 25 11:09:54 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Oct 25 11:05:54 2006 Subject: [Histonet] double staining In-Reply-To: <453F1ADE.91698D45@uctgsh1.uct.ac.za> Message-ID: <003e01c6f850$02ab7120$0300a8c0@domain.Premier> If I remember correctly from Chris van der Loos lecture and his book on multiple staining methods if the two antigens are co-localized then DAB is not a suitable chromagen. If the antigens are not co-localized then DAB would be fine. Please feel free to correct me if I'm remembering this wrong. I commonly use DAB with Fast Red for antigens that will stain different cells or different cellular components. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyler Sent: Wednesday, October 25, 2006 2:06 AM To: histonet Subject: [Histonet] double staining Morning to All Histonetters Request from one of the Med Techs I work with. Please can someone forward or suggest a method for double staining on tissue sections using DAB and AEC substrate. Thanks Marilyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1832 (20061025) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From wellborj <@t> mercyhealth.com Wed Oct 25 11:11:18 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Wed Oct 25 11:11:49 2006 Subject: [Histonet] lyve-1 staining Message-ID: Have you tried the Esoterix Lab? 1800-444-9111 Janci Wellborn, HTL(ASCP) Dakota Dunes, SD >>> "Horn, Hazel V" 10/25/2006 9:59 AM >>> I need to get this test done and I can't find anyone who does this. I have contacted ARUP, Mayo and Specialty Labs. Any ideas? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Wed Oct 25 11:20:29 2006 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Oct 25 11:20:46 2006 Subject: [Histonet] Joe's problems - a different take In-Reply-To: Message-ID: <20061025162029.76023.qmail@web52511.mail.yahoo.com> Hello everyone, I mentioned awhile ago that the managers should have stood behind Joe. It helps with doing our jobs if our side is asked before we get jumped on. It is so much easier to do our jobs if we are not always looking over our shoulders. Wondering who if friend or foe. Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bernaweston <@t> hotmail.com Wed Oct 25 11:26:18 2006 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Wed Oct 25 11:26:33 2006 Subject: [Histonet] nuclear fast red Message-ID: Does anyone out there use nuclear fast red at the grossing station on their small, clear/whitish specimens so that they can see them better during embedding? Eosin washes out during processing. Bernadette Weston HT Barberton Citizens Hosptital Barberton, OH _________________________________________________________________ Try Search Survival Kits: Fix up your home and better handle your cash with Live Search! http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=en-US&source=hmtagline From TownsendD <@t> childrensdayton.org Wed Oct 25 11:33:42 2006 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Oct 25 11:34:44 2006 Subject: [Histonet] nuclear fast red Message-ID: Hi, Bernadette I never used Nuclear fast red, but I found that hematoxylin is probably the best: a drop on the specimen will color it, it resists through processing and since you are going to stain with H&E anyway, it blends in with your staining and doesn't leave a different color on your slides. I also worked in a hospital where they used mercurochrome to mark there tissue, that will also resist through processing. Dolores From funderwood <@t> mcohio.org Wed Oct 25 11:42:49 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed Oct 25 11:43:19 2006 Subject: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Message-ID: Oh I don't know about that. I still think Kenny Rogers had an illegal substance on his hand in game 2 of the World Series. >>> "Bonner, Janet" 10/24/2006 6:27 PM >>> This leaves nothing for Friday, we'll be all worn out! :( ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rittman, Barry R Sent: Tue 10/24/2006 6:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it I would recommend that you use pistols at dawn and keep this between you two. I volunteer to bury the poorest shot. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 4:50 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Douglas: How am I "grasping at straws"? The thing about the demo equipment is irrelevant. Geoff stated that I sent him "a very nasty off list reply" to a 3 year old message without providing any detail. My point was that Geoff's characterization of me and my message was absolutely, 100% incorrect. Geoff has made my point about posting something negative like that on the listserver. Some people will believe it is fact without knowing the whole story. The free exchange of scientific information is an appropriate use for the listserver. Using it as a weapon to attack someone you don't like or to harm a company you are unhappy with is not. Maybe I am idealistic, but it certainly seems reasonable to me. David Douglas D Deltour wrote: >It seems like you are grasping at straws again. I am sure Geoff is not lying >in bed at night stewing over a post three years ago. All he said is that he >wouldn't buy a piece of equipment without a demo. He voiced his opinion. He >is not a "disgruntled and frustrated poster". It is time to move on. > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 4:34 PM >To: Geoff McAuliffe >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >Dear List: > >I now understand why Geoff was so offended by my harmless comments. >This also makes my point as to why it is so important NOT to post your >negative opinions about a company - or an individual - on the >listserver. You can read the messages below which were retrieved from >the Microscopy listserver archives. My message to Geoff was not >"off-list" it was sent to him and the listserver. I'm confident that >once you read my message to him you will see that it was not nasty at >all. There is always more to the story than the single line some >disgruntled and frustrated poster may choose to share with you. Geoff >has obviously been stewing over my message for more than 3 years! >Geoff's own reference makes my case for me. > >This was posted on the Microscopy Listserver: > >From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz >Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) >Subject: LM - question on inverted microscope, Hoffman and VAREL contrast >Dear users of ListServer, > >I kindly ask you for your advice concerning inverted microscope. We need >to ....... > >Therefore, we consider to purchase an inverted microscope but we are not >sure if to ask for that one which is equipped with relief contrasting >method. If yes, which would be the best? Hoffman modulation ...... > >As we do not have a possibility to try inverted microscope with relief >contrast, I would very appreciate your opinion/advice. > >Thank you. > >Rostislav Zemek >Czech Republic E-mail: rosta-at-acarus.entu.cas.cz > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Tue, 04 Mar 2003 11:58:32 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Ask the vendor to bring the instrument you are considering to your lab for a >demonstration. We always do this for expensive items. Any vendor who >will not >comply is not worth dealing with. > >Geoff > >From: David Henriks : henriks-at-southbaytech.com >Date: Tue, 04 Mar 2003 18:26:26 -0800 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast >Geoff: > >I can appreciate that anyone would prefer to have a vendor deliver an >instrument to their door to do an on-site demonstration, but you also >must realize that it is simply not possible in every instance. To say >that "Any vendor who will not comply is not worth dealing with" is quite >simplistic and offensive. I have nothing to do with this particular >customer, but I can certainly understand how several excellent >microscopy vendors may have difficulty justifying an on-site demo in the >Czech Republic. I personally have been to the Czech Republic several >times to demonstrate our systems, however, it can certainly not be >justified in every instance. To dismiss vendors so capriciously would >be doing a disservice to Dr. Zemek. Perhaps the company offering the >best solution at the best price has a better method of demonstrating its >performance than through an on-site demo. Technology these days is >pretty impressive. > >Speaking as a family business owner and microscopy vendor for nearly 40 >years, I guess I take it a bit personally when vendors are treated with >such disrespect. Scientists and vendors working together rather than as >adversaries is much more productive and serves the scientific community >much more effectively. > >Best regards- > >David > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Wed, 05 Mar 2003 09:56:45 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Hi David: > >David Henriks wrote: > >} Geoff: >} >} I can appreciate that anyone would prefer to have a vendor deliver an >} instrument to their door to do an on-site demonstration, but you also >} must realize that it is simply not possible in every instance. > >That may be true. > >} To say >} that "Any vendor who will not comply is not worth dealing with" is quite >} simplistic and offensive. > >Hey, times are very tight here in University research land. We spend our >money the >way we want to. We can't afford to buy equipment we have not tested. If >this offends >you, so be it. In 30 years in university science, I have never had a >vendor refuse to >demo a product, even an inexpensive one. > >} I have nothing to do with this particular >} customer, but I can certainly understand how several excellent >} microscopy vendors may have difficulty justifying an on-site demo in the >} Czech Republic. > >If that is the nature of their business, then that is the nature of their >business. The vendor runs his business the way he wants to, the customer >spends his >money the way he wants to. Capitalism. > >} I personally have been to the Czech Republic several >} times to demonstrate our systems, however, it can certainly not be >} justified in every instance. To dismiss vendors so capriciously would >} be doing a disservice to Dr. Zemek. > >No, Dr Zemek can make his own decisions. I was suggesting one course of >action for his >consideration. > >} Perhaps the company offering the >} best solution at the best price has a better method of demonstrating its >} performance than through an on-site demo. Technology these days is >} pretty impressive.Speaking as a family business owner and microscopy >vendor for >} nearly 40 > >} years, I guess I take it a bit personally when vendors are treated with >} such disrespect. > >Not disrespect, it is just the way things operate in my world. How is >'not buying >a product without a demo to see if it fits your lab's needs' disrespectful?? > >} Scientists and vendors working together rather than as >} adversaries is much more productive and serves the scientific community >} much more effectively. > >That is a very nice, general statement that no one would argue with. It >is also >180 degrees opposite of buying a product without knowing if it will >solve your >problem. > >Geoff > > > > >Geoff McAuliffe wrote: > > > >>Dear list: >> >> David Henriks wrote a very nice-sounding reply to the vendor issue >>we are all concerned about. I say nice-sounding because while it may >>be the ideal situation, it is not always a real world situation. Joe's >>troubles bear this out. >> Several years ago someone posted a querry on this listserver (or >>the Microscopy listserver, I am not sure which) about purchasing an >>expensive piece of equipment. I suggested he invite vendors to bring a >>demo to his lab to see if their equipment fit his needs. I also >>suggested that any vendor who would not supply a demo not be >>considered in his purchase plans. I got a very nasty off list reply >>from .......... David Henriks of South Bay Technologies. >> Actions speak louder than words. >> >>Geoff >> >>David Henriks wrote: >> >> >> >>>I missed the earliest part of the thread here, but from what I see it >>>seems that someone criticized a vendor on the Histonet and then was >>>reprimanded by his boss for doing so after the vendor complained. >>>Now, everyone wants to ban vendors from the Histonet. I am a vendor >>>and I belong to many listservers. End users seem to have a very >>>skewed view of what a vendor is and what motivates us. My main >>>business is not histology, although we do offer some products for the >>>industry. Yes, companies are in business to make money. No >>>question. To say that patient care is not even on their radar screen >>>is ridiculous. If the customer's need is to improve patient care, >>>then that is what a vendor is striving for. Vendors make money by >>>supplying what customers want. If you want tools for better patient >>>care, then that is what we provide. If your needs change, then our >>>products change. We respond to your needs. If, as you say, patient >>>care is not even on the vendor radar screen, then that probably means >>>that customers have not made that a high priority in what they say >>>they need. Vendors do not make money by producing ineffective >>>products that customers don't want. The best way to ensure that >>>customers get what they need and vendors produce products to meet >>>those needs is to have an open forum where these issues can be freely >>>discussed. That requires interchange between end users and vendors. >>>Yes, there should be limitations - vendors should not overtly market >>>their products on the Histonet. They should respond to postings with >>>viable solutions - even if they involve their own products. Vendors >>>should not use this as a forum to denigrate their competition. By >>>the same token, end users should not use this as a forum to air their >>>grievances about a product or a manufacturer. A single negative >>>comment about a vendor or his product could have a devastating effect >>>on their business. Perhaps your complaints are justified, but there >>>are better ways to go about having your concerns heard. Any credible >>>vendor would love to hear from you if you are having a problem with >>>one of their products. Unfortunately, most customers do not contact >>>the company. Sometimes when they do contact a company they reach the >>>wrong person and do not get a satisfactory response. I can >>>understand the frustration. However, sharing your frustration in >>>such a public forum is not right. Just as it would not be right for >>>you to criticize one of your colleagues on the Histonet. >>>There are always the few that think vendors are their enemies and >>>think that they are solely motivated by greed. The reality is that >>>vendors and end users are in this together. The people that figure >>>that out early on are generally the most successful and the one who >>>actually drive the development of new products, new techniques and >>>make significant strides in their field. To ban vendors from your >>>site would be regrettable and counterproductive. >>> >>>The listserver should be used for a free exchange of ideas and not as >>>a venue for criticism. Praise in public, criticize in private. >>> >>>Can't we all just get along? >>> >>>Best regards- >>> >>>David >>> >>> >>> >> >> > > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Wed Oct 25 11:52:32 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Oct 25 11:49:58 2006 Subject: [Histonet] nuclear fast red In-Reply-To: Message-ID: We use put some eosin in the last absolute alcohol on the processor. This seems to work without it washing out. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette Weston Sent: Wednesday, October 25, 2006 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nuclear fast red Does anyone out there use nuclear fast red at the grossing station on their small, clear/whitish specimens so that they can see them better during embedding? Eosin washes out during processing. Bernadette Weston HT Barberton Citizens Hosptital Barberton, OH _________________________________________________________________ Try Search Survival Kits: Fix up your home and better handle your cash with Live Search! http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=e n-US&source=hmtagline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Oct 25 11:50:41 2006 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Oct 25 11:50:56 2006 Subject: [Histonet] Gomori's trichrome problem Message-ID: <1872B4A455B7974391609AD8034C79FC0670B8@LBEXCH01.hchd.local> Hello to all in histoland. We are currently having a problem that has just cropped up within the last few weeks with our gomori's one step trichrome stain. It is being done as the procedure says, but when the slides come out of the one step solution and they are rinsed in 100% alcohol, they have a white filmy background. I think that it has something to do with the sections being on a charged slide. The only thing that we are not doing according to procedure is not going directly to 0.5% acetic acid solution after the one step solution. Any help will be appreciated. JACHO is in the house, I hope that they don't come in my area. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From histology.bc <@t> shaw.ca Wed Oct 25 11:52:23 2006 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Wed Oct 25 11:53:48 2006 Subject: [Histonet] nuclear fast red In-Reply-To: References: Message-ID: <453F9647.6070208@shaw.ca> As you say, the goal of applying a stain during processing is to non-specifically stain the tissue, so it can been seen during embedding. Nuclear fast red would not be a good choice for this purpose because as the name suggests this is a cationic dye and will stain nuclei, but nuclei make up only a small fraction of overall tissue mass. It will not stain connective tissues, muscle, etc. very intensely. I have been using a strong solution of eosin for this purpose (5 grams of eosin Y in 100 mL of absolute alcohol). 2-3 mL of the eosin solution in the last dehydrating alcohol will stain the tissues quite pink. The dye is not removed from the tissues as the subsequent processing fluids (xylene and paraffin wax) are not eosin solvents. All histology have eosin on hand ... and it is cheap! Happy embedding, Paul Kamloops, BC Canada Bernadette Weston wrote: > Does anyone out there use nuclear fast red at the grossing station on > their small, clear/whitish specimens so that they can see them better > during embedding? Eosin washes out during processing. > > Bernadette Weston HT > Barberton Citizens Hosptital > Barberton, OH > > _________________________________________________________________ > Try Search Survival Kits: Fix up your home and better handle your cash > with Live Search! > http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=en-US&source=hmtagline > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ddeibler <@t> centexpathlab.com Wed Oct 25 11:47:05 2006 From: ddeibler <@t> centexpathlab.com (David Deibler) Date: Wed Oct 25 11:54:13 2006 Subject: [Histonet] IHC position Message-ID: Hello, Central Texas Pathology Laboratory located in Waco Texas has an immediate opening For a full time Immmuno tech. Immunohistochemistry experience preferred. Our IHC department Is growing and we will soon be using three automated Dako machines. Excellent benefit package. Salary competitive with much larger cities. CTPL is a private lab owned by a group of pathologists Which has recently expanded. Although growing larger the atmosphere is still relaxed and the pathologists Are easy to work with .Please send resume to: 3115 Pine Avenue Suite 108 Waco, Texas 76708 Attn: IHC Contact Cynthia Bulmer HT (ASCP) Q IHC IHC supervisor (254) 752-9621 ext 247 Or David Deibler HT (ASCP) Histology supervisor (254) 752-9621 ext 219 Email : ddeibler@centexpathlab.com From JMitchell <@t> uwhealth.org Wed Oct 25 11:58:30 2006 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Wed Oct 25 11:58:42 2006 Subject: [Histonet] Gomori's trichrome problem In-Reply-To: <1872B4A455B7974391609AD8034C79FC0670B8@LBEXCH01.hchd.local> Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB10225AA43@uwhis-xchng4.uwhis.hosp.wisc.edu> Just curious - are you purchasing your one-step trichrome solution or making it yourself? I have had problems in the recent past w/purchased one-step trichrome. And not just from one vendor but several that shall for the time being remain un-named suppliers. Jean Mitchell UWHC Neuromuscular Laboratory Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, October 25, 2006 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori's trichrome problem Hello to all in histoland. We are currently having a problem that has just cropped up within the last few weeks with our gomori's one step trichrome stain. It is being done as the procedure says, but when the slides come out of the one step solution and they are rinsed in 100% alcohol, they have a white filmy background. I think that it has something to do with the sections being on a charged slide. The only thing that we are not doing according to procedure is not going directly to 0.5% acetic acid solution after the one step solution. Any help will be appreciated. JACHO is in the house, I hope that they don't come in my area. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Gladney <@t> se.amedd.army.mil Wed Oct 25 12:13:59 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Wed Oct 25 12:14:52 2006 Subject: [Histonet] nuclear fast red In-Reply-To: Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302011A2768@amedmlsermc133> We use Eosin in the last absolute alcohol on the processor. This has worked wonderfully for us over the years without any wash out. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette Weston Sent: Wednesday, October 25, 2006 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nuclear fast red Does anyone out there use nuclear fast red at the grossing station on their small, clear/whitish specimens so that they can see them better during embedding? Eosin washes out during processing. Bernadette Weston HT Barberton Citizens Hosptital Barberton, OH _________________________________________________________________ Try Search Survival Kits: Fix up your home and better handle your cash with Live Search! http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&loca le=en-US&source=hmtagline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlgiebel <@t> vcu.edu Wed Oct 25 12:16:59 2006 From: mlgiebel <@t> vcu.edu (Mary L Giebel/FS/VCU) Date: Wed Oct 25 12:17:07 2006 Subject: [Histonet] End Goodbye comments Message-ID: Kallye, I thought that Joe was banned from writing to t was still employed with his current company. That was the "Goodbye" message. I feel sure Joe would be taking an ac part in the discussions if he could...and still eat, sleep under a roo Mary Lee - To: Barry.R.Rittman@uth From: "Kallye Walther" From: "Rittman, Barry >To: Subject: [Histonet] End Goodbye comments >Dat > >There will never be an id >sense and tolerance for b >situation individually as > >Sometimes both vendors > > > > >his > >Barry > >______ _______________________ 5F_ >Histonet mailing li >Histonet@lists.utsouthwestern.edu >[1]http://lists ______ _______________________ 5F_ _______________________ 5F_ Stay in touch with old friends and meet new Spaces [2]http://clk.atdmt.com/MSN/go/msnnkwsp0070000001msn/direct/01/?hr ef=http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_u r ________ _______________________ 5F_ Histonet mailing list Hist [3]http://lists.utsouthwestern.ed References 1. 3D"http://lists.=/ 2. 3D"http://clk.atdmt.com/MSN/go/ms 3. 3D"http://lists.utsouthwestern.edu=/ From Kathy.Johnston <@t> CLS.ab.ca Wed Oct 25 12:21:28 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Wed Oct 25 12:22:11 2006 Subject: [Histonet] Gomori's trichrome problem In-Reply-To: <1872B4A455B7974391609AD8034C79FC0670B8@LBEXCH01.hchd.local> Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE01365447@mail1.calgary.com> Hi Allison! Without knowing which dyes your kit is employing, I would have to say the lack of an aqueous rinse of some sort is your culprit. Regardless whether you have an Aniline Blue or Light green collagen stain component, all of the dyes in your typical Gomori one step are far more soluble in water than alcohol. The Aniline Blue is probably the worst of the batch being barely soluble in alcohol. We use a 1% Acetic Acid rinse (approx 5 dips) to rinse most of the dyes off before putting it in 95% ETOH then 100% ETOH and up to Xylene. We also have charged slides in our runs and they work fine for us. If you don't want to stock that extra reagent, just give them a quick swish in dH2O before you run them up thru ETOH. You will also see this phenomenon in your Retics if you forget to rinse off your Nuclear fast red (found that out the hard way!). Hope this might help! Good Luck! Kathy Johnston Tech II Special Stains Anatomic Pathology Calgary Laboratory Services #9 - 3535 Research Road NW Calgary, AB. 403-770-3572 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: October 25, 2006 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori's trichrome problem Hello to all in histoland. We are currently having a problem that has just cropped up within the last few weeks with our gomori's one step trichrome stain. It is being done as the procedure says, but when the slides come out of the one step solution and they are rinsed in 100% alcohol, they have a white filmy background. I think that it has something to do with the sections being on a charged slide. The only thing that we are not doing according to procedure is not going directly to 0.5% acetic acid solution after the one step solution. Any help will be appreciated. JACHO is in the house, I hope that they don't come in my area. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From DBotsfor <@t> wrh.on.ca Wed Oct 25 12:33:15 2006 From: DBotsfor <@t> wrh.on.ca (Botsford, Daniel) Date: Wed Oct 25 12:37:54 2006 Subject: [Histonet] nuclear fast red Message-ID: <331EE6D48DDFD51189E500508BBD360806D9848F@mail.wrh.on.ca> Hello Bernadette Weston, We add 5ml of eosin the the 100% alcohol of the tissue processer.This stains the tissue pink to give contrast in embedding. As well when cutting large tissue like uterus, as you cut into the tissue it gives a clear red outline of the tssue so you know you have a complete section. Sincerely Daniel Botsford Windsor Regional Hospital 1995 Lens Avenue Windsor, Ontario N8W 1L9 519-254-5577 ext 52373 519-254-6861 fax dbotsfor@wrh.on.ca Previous Does anyone out there use nuclear fast red at the grossing station on their small, clear/whitish specimens so that they can see them better during embedding? Eosin washes out during processing From Tamara.Franz-Odendaal <@t> msvu.ca Wed Oct 25 12:41:48 2006 From: Tamara.Franz-Odendaal <@t> msvu.ca (Tamara Franz-Odendaal) Date: Wed Oct 25 12:49:24 2006 Subject: [Histonet] sections curling Message-ID: Kemlo I section decalcified bone in paraffin routinely. I cut 3-8um depending on the tissue. Curling and wrinkling may have to do with humidity in the room, we find this often. Also try putting your block in the freezer overnight if you don't already. I do not use disposable blades. Hope this helps Tamara Tamara Franz-Odendaal Mount Saint Vincent University Halifax, NS, Canada Tamara Franz-Odendaal (PhD) NSERC University Faculty Awardee Biology Dept, Mount Saint Vincent University 166 Bedford Highway Halifax, NS, B3M 2J6 Canada Tel: 902 - 457 6140 Fax: 902 - 457 6455 >>> "Kemlo Rogerson" 10/25/2006 4:45 AM >>> Hello, sorry for the simplistic question. We are having a lot of problem with some sections curling and wrinkling. this is decalcified bone in parafin. We are using a sliding microtome with disposable blades. is it the blades? This is my first use of disposables. It seems like the answer is very simple, but I have drawn a blank. Bet it's the processing, but it could be the angle of the blade. Revisit your processing schedule and try altering the angle of attack for your blade (what is the correct name for that angle?). Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Nobody grows old merely by living a number of years. We grow old by deserting our ideals. Years may wrinkle the skin, but to give up enthusiasm wrinkles the soul. --Samuel Ullman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Oct 25 12:57:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Oct 25 12:58:10 2006 Subject: [Histonet] Double Immunohistochemical staining Message-ID: <6.0.0.22.1.20061025113314.01b36f58@gemini.msu.montana.edu> Dear Liz and others, Chris van der Loos has always prefered using a peroxidase system AEC chromogen with alkaline phosphatase Vector blue to see purple colocalization. Liz is correct on the DAB/AEC issue from his book, and her use of DAB with Fast red is ideal. DAB combined with AEC in general is a poorer color combination. AEC is an orangish red and DAB is brown and contrast of these colors can be less appealing than using a red chromogen with magenta red - pinkish red tones to give better contrast and color separation. Orange red fights with the brown and we didn't like it. An alkaline phosphatase method using any of these chromogens (new fuchsin, fast red or very sensitive Permanent red (a van der Loos favorite)) with brownish DAB will avoid the clash of colors with AEC. Enhanced DAB could be tried if one wants a black chromogen, or even a darker brown with new DAB enhancers. He also prefers to use one alkaline phosphatase method and then a peroxidase method. If one uses two peroxidase methods, then another peroxidase block should be done between the two HRP steps to ensure quenching of first HRP enzyme before using another HRP in with second antibody. This is not necessary for alk phos and then peroxidase. Liz wrote: If I remember correctly from Chris van der Loos lecture and his book on multiple staining methods if the two antigens are co-localized then DAB is not a suitable chromagen. If the antigens are not co-localized then DAB would be fine. Please feel free to correct me if I'm remembering this wrong. I commonly use DAB with Fast Red for antigens that will stain different cells or different cellular components. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From HornHV <@t> archildrens.org Wed Oct 25 12:59:46 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Oct 25 13:00:03 2006 Subject: [Histonet] nuclear fast red In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6E4E@EMAIL.archildrens.org> No we don't use NFR but we do use eosin and have not had a problem with it washing out. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette Weston Sent: Wednesday, October 25, 2006 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nuclear fast red Does anyone out there use nuclear fast red at the grossing station on their small, clear/whitish specimens so that they can see them better during embedding? Eosin washes out during processing. Bernadette Weston HT Barberton Citizens Hosptital Barberton, OH _________________________________________________________________ Try Search Survival Kits: Fix up your home and better handle your cash with Live Search! http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&loca le=en-US&source=hmtagline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From gcallis <@t> montana.edu Wed Oct 25 13:28:17 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Oct 25 13:28:34 2006 Subject: [Histonet] Ergonomic woes with microtomy Message-ID: <6.0.0.22.1.20061025121147.01b21658@gemini.msu.montana.edu> We built up the handle of fly wheel and coarse advance wheel with taped on bubble wrap. A tennis ball is fairly large, but some people use bicycle handle grips (spongy, thick) which slip over microtome handles easily. We tried racquet balls also, but didn't like the feel although some use them successfully. Also, do NOT rock the fly wheel when trimming, this causes the wrist to bend up and down (watch yourself do this!) and is a unwanted but easily avoidable repetitive motion. Don't say you can't learn to do this, it takes only a few times to relearn a new motion. Jan Minshew from Leica would agree, she is an ergonomic expert at Leica and teaches this. Also, grasp the handles so you never bend you wrist, hold the handles with palms facing the ends rather than grasping the handle like a bicycler. This keeps the wrists rigid and straight for both trimming and sectioning. No resting arms on counter or get arm rests, use a decent chair, feet squarely on floor or use a foot rest, and all tools within easy reach. Replacement of waterbath so it is on an L shaped counter next to you rather than next to microtome prevents some unneeded twisting and stretching. Learn to exercise and stretch you hands, fingers, wrists to relieve stresses. This wonderful ergonomic forceps from Surgipath and others (have holes in handles) are very easy to use if you find you don't like the forceps that have opposition open/close mechanism. Automated microtomes are wonderful if you can buy one and certainly on our wish list. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From SBarnes <@t> elch.org Wed Oct 25 13:49:58 2006 From: SBarnes <@t> elch.org (Sue Barnes) Date: Wed Oct 25 13:50:05 2006 Subject: [Histonet] prefer fixative vs formalin Message-ID: We use Prefer in the surgery area and only have formalin on the tissue processor. We set a delay so that the tissue is in formalin for approximately 6 hours. The time in prefer is a couple of hours unless the surgery is done on the weekend. We also use the Ventana for IHC and have had no problems with the staining. We tried to use only Prefer and had some problems with the IHC but have heard from others who use only Prefer that you have to work with them a little. There is no need to do retrieval with only Prefer. We send some specimens out for prognostic markers and they require formalin fixation, but they have had no problems with our tissues. Hope this helps -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heather Renko Sent: Monday, October 23, 2006 8:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prefer fixative vs formalin Hello Fellow Histonetters, I am trying to eliminate some of the formalin in our lab and my safety officer has suggested that we can have our outpatient facilities and surgery department send our specimens to us in prefer and then transfer to formalin in our processor. Any experience and or thoughts on this. Additionally, what is your experience with switching over completely to prefer?? We run a normal overnight processing, no microwave processing and we do H&E, special stains, and immunohistochemistry on the Ventana platform. Thank you in advance. --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Oct 25 14:21:15 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Oct 25 14:21:20 2006 Subject: [Histonet] Gomori's trichrome problem In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE01365447@mail1.calgary.com> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E3FEA@exchsrv01.barrynet.barry.edu> Distilled water is a poor rinse for trichromes; 0.5% aqueous acetic acid is MUCH better. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Johnston@CLS.ab.ca Sent: Wednesday, October 25, 2006 1:21 PM To: Allison_Scott@hchd.tmc.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gomori's trichrome problem Hi Allison! Without knowing which dyes your kit is employing, I would have to say the lack of an aqueous rinse of some sort is your culprit. Regardless whether you have an Aniline Blue or Light green collagen stain component, all of the dyes in your typical Gomori one step are far more soluble in water than alcohol. The Aniline Blue is probably the worst of the batch being barely soluble in alcohol. We use a 1% Acetic Acid rinse (approx 5 dips) to rinse most of the dyes off before putting it in 95% ETOH then 100% ETOH and up to Xylene. We also have charged slides in our runs and they work fine for us. If you don't want to stock that extra reagent, just give them a quick swish in dH2O before you run them up thru ETOH. You will also see this phenomenon in your Retics if you forget to rinse off your Nuclear fast red (found that out the hard way!). Hope this might help! Good Luck! Kathy Johnston Tech II Special Stains Anatomic Pathology Calgary Laboratory Services #9 - 3535 Research Road NW Calgary, AB. 403-770-3572 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: October 25, 2006 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori's trichrome problem Hello to all in histoland. We are currently having a problem that has just cropped up within the last few weeks with our gomori's one step trichrome stain. It is being done as the procedure says, but when the slides come out of the one step solution and they are rinsed in 100% alcohol, they have a white filmy background. I think that it has something to do with the sections being on a charged slide. The only thing that we are not doing according to procedure is not going directly to 0.5% acetic acid solution after the one step solution. Any help will be appreciated. JACHO is in the house, I hope that they don't come in my area. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. 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If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From amosbrooks <@t> gmail.com Wed Oct 25 14:33:36 2006 From: amosbrooks <@t> gmail.com (amosbrooks@gmail.com) Date: Wed Oct 25 14:36:17 2006 Subject: [Histonet] Vendor Perspective In-Reply-To: <453ECE3A.1050203@southbaytech.com> References: <582736990610241704p2197e82cwcc89ed369612d404@mail.gmail.com> <453ECE3A.1050203@southbaytech.com> Message-ID: <1407963698-1161804962-cardhu_blackberry.rim.net-1397029553-@bwe020-cell00.bisx.prod.on.blackberry> David, I think we are just bound to disagree on this one. I certainly do see your perspective, but I still think that good and bad opinions are valuable to the group as a whole. You don't see it that way and that is OK. Just remember that it is still bound to happen and the majority of the Histonet seems to be glad to hear both sides of it. I would advise a vendor to keep tabs on what is said on the Histonet so they can be prepared to fully explain problems that are being discussed when a customer inquires, and they will inquire! Thanks, Amos Brooks Sent from my BlackBerry? wireless device -----Original Message----- From: David Henriks Date: Tue, 24 Oct 2006 19:38:50 To:Amos Brooks Cc:histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Vendor Perspective Amos: Everyone seems to misunderstand what I said.? I never said not to share your opinion.? I said to share it in private.? I would support stating your negative opinions on the listserver if the entire conversation took place on the listserver.? I think it is unfair for someone to toss out a negative opinion without including the background and without the vendor having the opportunity to respond.? I don't think anyone wants to be inundated with all of the details of someone's individual situation, however I think it is necessary to put the comments in context.? As an example, one of the listserve members made some negative personal comments about me.? When I defended myself and tried to provide the complete picture I was greeted with people telling me I was "grasping at straws" and told to "shut the *&?! up".?? With that in mind, it seems that a vendor won't get a fair shake here even to defend his good name.? It seems to me that it makes more sense to send the negative comments off line and let the people who have the real interest sort it out.? Alternatively, you need to happily accept the entire thread from anyone who wants to post without castigating them both on and off line.? Also, it should be noted that one hot headed remark certainly can be slanderous - particularly when made openly to a forum of thousands of potential customers, employers and colleagues. Even within the first amendment there are limits to what can be said.? Although, I don't see this as so much a 1st amendment issue as I do an issue of common decency.? Best regards- David Amos Brooks wrote: David, ??? I have to respectfully disagree. This is a group forum, and while I feel stifled at times about offering my experiences with products, it would entirely defeat the purpose of this forum if only the sunshiny glowing references were permitted. It's just not a reflection of reality. Not everyone will like "Product A" and they have every right to say so. ??? If you feel there is any basis to fight this First Ammendment case I think you should start with the bumperstickers of car manufacturer logos being watered down. (see: http://www.customnames.com/images/calvin_Ford.jpg: ) (Apologies to Ford Royer, tough thread my friend) If you can get anywhere with that then I'm sure you will see people being more cautious about saying anything bad about your products. They will probably all be moving to North Korea for more personal freedom too. ??? The ideal thing for the vendor to do would be to contact the person making the complaint and see what can be done to fix the problem. Accepting the fact that it is entirely possible that the customer may be too far gone to do anything about. No harm in trying though. ??? Now it must be said that there is a limit to expressing a negative opinion and simply slandreing or bashing a company. This is unacceptable too and in this case the vendor needs to do what they need to do to stay alive. Honestly though one hot headed remark does not slander make. If it really bugs you find a customer you have a good relationship with to counter the claim. ??? I have to disagree emphatically with any call to have vendors bumped off the Histonet. As a matter of fact I think we need more, but they need to understand the basics of the Histonet. I have had Vendors and Sales Reps give very good advice and have benefitted very much from their help over the years. But it is imperative that they keep in mind the purpose of this forum. It is not to be used as a sales platform. If you are helpful and answer customer's questions they will remember you. If you hunt them down and chastize them for daring to not like your product ... they will remember you. Cheers, Amos Brooks -------------------------------------------- From: David Henriks <henriks@southbaytech.com>: Subject: Re: [Histonet] Re: goodbye - vendor perspective To: Douglas D Deltour <doug@ppspath.com>: Cc: histonet@lists.utsouthwestern.edu: Message-ID: <453E48AE.2050505@southbaytech.com>: Content-Type: text/plain; charset=ISO-8859-1; format=flowed John should answer Jane's question. John should answer Jane's question directly to Jane. John? should not post his comments to the listserver as a whole. -- David Henriks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu: http://lists.utsouthwestern.edu/mailman/listinfo/histonet: -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: 949-492-2600 Toll-free in the USA: 800-728-2233 FAX: 949-492-1499 email: henriks+AEA-southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com: . The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling 949-492-2600 and delete this message from your system. From ASenn <@t> mercy.pmhs.org Wed Oct 25 14:48:58 2006 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Wed Oct 25 14:49:07 2006 Subject: [Histonet] Nuclear Fast Red Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A62@pmhs2kxch03> Message: 18 Date: Wed, 25 Oct 2006 12:26:18 -0400 From: "Bernadette Weston" Subject: [Histonet] nuclear fast red To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Does anyone out there use nuclear fast red at the grossing station on their small, clear/whitish specimens so that they can see them better during embedding? Eosin washes out during processing. Bernadette Weston HT Barberton Citizens Hosptital Barberton, OH -----------------------------We use Hematoxylin -----Amy ------------------------ From kimtournear <@t> yahoo.com Wed Oct 25 14:51:40 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Oct 25 14:51:47 2006 Subject: [Histonet] Waterbaths Message-ID: <20061025195140.51987.qmail@web37707.mail.mud.yahoo.com> Hi all, I'm in the process of buying new water baths....has/is anyone using SurgiPath's? Is there any one vendor better than another? Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From wellborj <@t> mercyhealth.com Wed Oct 25 15:09:58 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Wed Oct 25 15:10:27 2006 Subject: [Histonet] nuclear fast red Message-ID: We use saffron - the water is the differentiator so the saffrom comes out during the staining of the tissues. Happy Cutting, Janci Wellborn, HTL (ASCP) >>> "Paul Bradbury" 10/25/2006 11:52 AM >>> As you say, the goal of applying a stain during processing is to non-specifically stain the tissue, so it can been seen during embedding. Nuclear fast red would not be a good choice for this purpose because as the name suggests this is a cationic dye and will stain nuclei, but nuclei make up only a small fraction of overall tissue mass. It will not stain connective tissues, muscle, etc. very intensely. I have been using a strong solution of eosin for this purpose (5 grams of eosin Y in 100 mL of absolute alcohol). 2-3 mL of the eosin solution in the last dehydrating alcohol will stain the tissues quite pink. The dye is not removed from the tissues as the subsequent processing fluids (xylene and paraffin wax) are not eosin solvents. All histology have eosin on hand ... and it is cheap! Happy embedding, Paul Kamloops, BC Canada Bernadette Weston wrote: > Does anyone out there use nuclear fast red at the grossing station on > their small, clear/whitish specimens so that they can see them better > during embedding? Eosin washes out during processing. > > Bernadette Weston HT > Barberton Citizens Hosptital > Barberton, OH > > _________________________________________________________________ > Try Search Survival Kits: Fix up your home and better handle your cash > with Live Search! > http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=en-US&source=hmtagline > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Oct 25 15:20:49 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Oct 25 15:25:49 2006 Subject: [Histonet] Waterbaths References: <20061025195140.51987.qmail@web37707.mail.mud.yahoo.com> Message-ID: <898D946569A27444B65667A49C074052D0DE3A@mailbe06.mc.vanderbilt.edu> Hi Kim, I am not currently using a Surgipath bath, but I absolutely love them! I like the rectanglar one. If my current dinosaur ever goes out, I'll be on the phone, P.O. in hand. I am sure you can get a demo. I tried a demo unit before I bought my first one (a long time ago in a place far away). **not in any way intended to discount another manufacturer's waterbath, I just really like this one! Surgipath also has always come through in the customer service arena, as well. Good luck, I'm going into the Bunker now, Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Tournear Sent: Wed 10/25/2006 2:51 PM To: Histonet Subject: [Histonet] Waterbaths Hi all, I'm in the process of buying new water baths....has/is anyone using SurgiPath's? Is there any one vendor better than another? Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barb94117 <@t> msn.com Wed Oct 25 15:30:27 2006 From: barb94117 <@t> msn.com (barbara albert) Date: Wed Oct 25 15:31:09 2006 Subject: [Histonet] Ergonomic woes with microtomy In-Reply-To: <6.0.0.22.1.20061025121147.01b21658@gemini.msu.montana.edu> Message-ID: One small note--I purchased a small pamphlet written by a PT a few years ago called "Hand Exercises for Knitters" which I find useful even though I knit only rarely any more. My ex-husband who has weakness on one side as the result of a stroke also likes the exercises. If anyone is interested, I'll bring the book from home and post whatever publishing information is available. Barbara Albert UCSF Medical Center San Francisco >From: Gayle Callis >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ergonomic woes with microtomy >Date: Wed, 25 Oct 2006 12:28:17 -0600 > >We built up the handle of fly wheel and coarse advance wheel with taped on >bubble wrap. A tennis ball is fairly large, but some people use bicycle >handle grips (spongy, thick) which slip over microtome handles easily. We >tried racquet balls also, but didn't like the feel although some use them >successfully. > >Also, do NOT rock the fly wheel when trimming, this causes the wrist to >bend up and down (watch yourself do this!) and is a unwanted but easily >avoidable repetitive motion. Don't say you can't learn to do this, it >takes only a few times to relearn a new motion. Jan Minshew from Leica >would agree, she is an ergonomic expert at Leica and teaches this. Also, >grasp the handles so you never bend you wrist, hold the handles with palms >facing the ends rather than grasping the handle like a bicycler. This >keeps the wrists rigid and straight for both trimming and sectioning. > >No resting arms on counter or get arm rests, use a decent chair, feet >squarely on floor or use a foot rest, and all tools within easy reach. >Replacement of waterbath so it is on an L shaped counter next to you rather >than next to microtome prevents some unneeded twisting and stretching. >Learn to exercise and stretch you hands, fingers, wrists to relieve >stresses. > >This wonderful ergonomic forceps from Surgipath and others (have holes in >handles) are very easy to use if you find you don't like the forceps that >have opposition open/close mechanism. > >Automated microtomes are wonderful if you can buy one and certainly on our >wish list. > >Good luck >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histosci <@t> shentel.net Wed Oct 25 15:31:56 2006 From: histosci <@t> shentel.net (HSRL) Date: Wed Oct 25 15:32:07 2006 Subject: [Histonet] Waterbaths In-Reply-To: <898D946569A27444B65667A49C074052D0DE3A@mailbe06.mc.vanderbilt.edu> Message-ID: <001d01c6f874$a0b159b0$0700a8c0@HSRLMAIN> Yep, I have to agree- Surgipath's waterbaths can't be beat. Tom Tom Galati Laboratory Director HSRL, Inc.- A GLP Histopathology Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: Wednesday, October 25, 2006 3:21 PM To: Kim Tournear; histonet Subject: RE: [Histonet] Waterbaths Hi Kim, I am not currently using a Surgipath bath, but I absolutely love them! I like the rectanglar one. If my current dinosaur ever goes out, I'll be on the phone, P.O. in hand. I am sure you can get a demo. I tried a demo unit before I bought my first one (a long time ago in a place far away). **not in any way intended to discount another manufacturer's waterbath, I just really like this one! Surgipath also has always come through in the customer service arena, as well. Good luck, I'm going into the Bunker now, Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Tournear Sent: Wed 10/25/2006 2:51 PM To: Histonet Subject: [Histonet] Waterbaths Hi all, I'm in the process of buying new water baths....has/is anyone using SurgiPath's? Is there any one vendor better than another? Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judi.ford <@t> jax.org Wed Oct 25 15:39:20 2006 From: judi.ford <@t> jax.org (judi.ford@jax.org) Date: Wed Oct 25 15:40:27 2006 Subject: [Histonet] Mouse Tissue Problem Message-ID: <6941674.1161808760672.JavaMail.ocsadmin@jcs-mid-prod.jax.org> Hi Everyone, Our lab has encountered a problem with a batch of mouse tissue processed recently. We receive tissue from various research labs and it is usually either fixed in Telly's or Bouin's for 24 hrs before processing. Nothing unusual was noticed until facing this batch of blocks. Some blocks faced fine while others, like muscle and tumors, shredded and the tissue was soft and spongy (light colored in the middle). The blocks I have faced fine and were hydrated on an ice tray prior to sectioning. In the past I've hydrated bouin's fixed blocks for a couple of days and never had a problem with cutting. These blocks were hydrated in the frig overnight, which is my usual pattern, and then cut the next day. I'm having a horrible time cutting the tissue. Intestines (not impacted with feces) are not cutting well (very compressed with a new knife) and the tissue breaks away from the paraffin both in the block and on the waterbath. The surface of the faced tissue appears wavy after hydration. My water bath temperature is 40 degrees centigrade. We've been discussing the following as possible causes: inadequate fixation, fixative mixed wrong, inadequate levels of solutions in the processor (although tissue on the top layer came out fine), tissue not cleared properly, etc. I hoping someone may have some ideas on how to narrow down our troubleshooting with this problem. Thanks in advance. Judi Ford HIstotechnologist The Jackson Laboratory Bar Harbor, Me 04609 From judi.ford <@t> jax.org Wed Oct 25 15:39:57 2006 From: judi.ford <@t> jax.org (judi.ford@jax.org) Date: Wed Oct 25 15:40:29 2006 Subject: [Histonet] Mouse Tissue Problem Message-ID: <11399107.1161808797603.JavaMail.ocsadmin@jcs-mid-prod.jax.org> Hi Everyone, Our lab has encountered a problem with a batch of mouse tissue processed recently. We receive tissue from various research labs and it is usually either fixed in Telly's or Bouin's for 24 hrs before processing. Nothing unusual was noticed until facing this batch of blocks. Some blocks faced fine while others, like muscle and tumors, shredded and the tissue was soft and spongy (light colored in the middle). The blocks I have faced fine and were hydrated on an ice tray prior to sectioning. In the past I've hydrated bouin's fixed blocks for a couple of days and never had a problem with cutting. These blocks were hydrated in the frig overnight, which is my usual pattern, and then cut the next day. I'm having a horrible time cutting the tissue. Intestines (not impacted with feces) are not cutting well (very compressed with a new knife) and the tissue breaks away from the paraffin both in the block and on the waterbath. The surface of the faced tissue appears wavy after hydration. My water bath temperature is 40 degrees centigrade. We've been discussing the following as possible causes: inadequate fixation, fixative mixed wrong, inadequate levels of solutions in the processor (although tissue on the top layer came out fine), tissue not cleared properly, etc. I hoping someone may have some ideas on how to narrow down our troubleshooting with this problem. Thanks in advance. Judi Ford HIstotechnologist The Jackson Laboratory Bar Harbor, Me 04609 From DALEY_C <@t> palmer.edu Wed Oct 25 15:43:36 2006 From: DALEY_C <@t> palmer.edu (Clover Daley) Date: Wed Oct 25 15:43:46 2006 Subject: [Histonet] Sodium Pentobarbital supplier Message-ID: Many of the suppliers that we have purchased sodium pentobarbital/Nembutal no longer carry it. We use it for rats before perfusion. Please advise as to a supplier that still carries it. Thank you, Clover Daley Palmer Center for Chiropractic Research 741 Brady Street Davenport, Iowa 52803 From Janet.Bonner <@t> FLHOSP.ORG Wed Oct 25 16:05:54 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Oct 25 16:07:11 2006 Subject: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it References: Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A664@fhosxchmb006.ADVENTISTCORP.NET> Ya think??!! @:o ________________________________ From: Fred Underwood [mailto:funderwood@mcohio.org] Sent: Wed 10/25/2006 12:42 PM To: Bonner, Janet; histonet@lists.utsouthwestern.edu; Barry.R.Rittman@uth.tmc.edu Subject: RE: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Oh I don't know about that. I still think Kenny Rogers had an illegal substance on his hand in game 2 of the World Series. >>> "Bonner, Janet" 10/24/2006 6:27 PM >>> This leaves nothing for Friday, we'll be all worn out! :( ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rittman, Barry R Sent: Tue 10/24/2006 6:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it I would recommend that you use pistols at dawn and keep this between you two. I volunteer to bury the poorest shot. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 4:50 PM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Douglas: How am I "grasping at straws"? The thing about the demo equipment is irrelevant. Geoff stated that I sent him "a very nasty off list reply" to a 3 year old message without providing any detail. My point was that Geoff's characterization of me and my message was absolutely, 100% incorrect. Geoff has made my point about posting something negative like that on the listserver. Some people will believe it is fact without knowing the whole story. The free exchange of scientific information is an appropriate use for the listserver. Using it as a weapon to attack someone you don't like or to harm a company you are unhappy with is not. Maybe I am idealistic, but it certainly seems reasonable to me. David Douglas D Deltour wrote: >It seems like you are grasping at straws again. I am sure Geoff is not lying >in bed at night stewing over a post three years ago. All he said is that he >wouldn't buy a piece of equipment without a demo. He voiced his opinion. He >is not a "disgruntled and frustrated poster". It is time to move on. > > >Douglas D. Deltour HT(ASCP) >Histology Supervisor >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >(803)252-1913 >Fax (803)254-3262 > >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David >Henriks >Sent: Tuesday, October 24, 2006 4:34 PM >To: Geoff McAuliffe >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: goodbye - vendor perspective > >Dear List: > >I now understand why Geoff was so offended by my harmless comments. >This also makes my point as to why it is so important NOT to post your >negative opinions about a company - or an individual - on the >listserver. You can read the messages below which were retrieved from >the Microscopy listserver archives. My message to Geoff was not >"off-list" it was sent to him and the listserver. I'm confident that >once you read my message to him you will see that it was not nasty at >all. There is always more to the story than the single line some >disgruntled and frustrated poster may choose to share with you. Geoff >has obviously been stewing over my message for more than 3 years! >Geoff's own reference makes my case for me. > >This was posted on the Microscopy Listserver: > >From: Rostislav Zemek : rosta-at-acarus.entu.cas.cz >Date: Tue, 4 Mar 2003 13:40:01 +0100 (CET) >Subject: LM - question on inverted microscope, Hoffman and VAREL contrast >Dear users of ListServer, > >I kindly ask you for your advice concerning inverted microscope. We need >to ....... > >Therefore, we consider to purchase an inverted microscope but we are not >sure if to ask for that one which is equipped with relief contrasting >method. If yes, which would be the best? Hoffman modulation ...... > >As we do not have a possibility to try inverted microscope with relief >contrast, I would very appreciate your opinion/advice. > >Thank you. > >Rostislav Zemek >Czech Republic E-mail: rosta-at-acarus.entu.cas.cz > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Tue, 04 Mar 2003 11:58:32 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Ask the vendor to bring the instrument you are considering to your lab for a >demonstration. We always do this for expensive items. Any vendor who >will not >comply is not worth dealing with. > >Geoff > >From: David Henriks : henriks-at-southbaytech.com >Date: Tue, 04 Mar 2003 18:26:26 -0800 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast >Geoff: > >I can appreciate that anyone would prefer to have a vendor deliver an >instrument to their door to do an on-site demonstration, but you also >must realize that it is simply not possible in every instance. To say >that "Any vendor who will not comply is not worth dealing with" is quite >simplistic and offensive. I have nothing to do with this particular >customer, but I can certainly understand how several excellent >microscopy vendors may have difficulty justifying an on-site demo in the >Czech Republic. I personally have been to the Czech Republic several >times to demonstrate our systems, however, it can certainly not be >justified in every instance. To dismiss vendors so capriciously would >be doing a disservice to Dr. Zemek. Perhaps the company offering the >best solution at the best price has a better method of demonstrating its >performance than through an on-site demo. Technology these days is >pretty impressive. > >Speaking as a family business owner and microscopy vendor for nearly 40 >years, I guess I take it a bit personally when vendors are treated with >such disrespect. Scientists and vendors working together rather than as >adversaries is much more productive and serves the scientific community >much more effectively. > >Best regards- > >David > >From: Geoff McAuliffe : mcauliff-at-umdnj.edu >Date: Wed, 05 Mar 2003 09:56:45 -0500 >Subject: Re: LM - question on inverted microscope, Hoffman and VAREL >contrast > >Hi David: > >David Henriks wrote: > >} Geoff: >} >} I can appreciate that anyone would prefer to have a vendor deliver an >} instrument to their door to do an on-site demonstration, but you also >} must realize that it is simply not possible in every instance. > >That may be true. > >} To say >} that "Any vendor who will not comply is not worth dealing with" is quite >} simplistic and offensive. > >Hey, times are very tight here in University research land. We spend our >money the >way we want to. We can't afford to buy equipment we have not tested. If >this offends >you, so be it. In 30 years in university science, I have never had a >vendor refuse to >demo a product, even an inexpensive one. > >} I have nothing to do with this particular >} customer, but I can certainly understand how several excellent >} microscopy vendors may have difficulty justifying an on-site demo in the >} Czech Republic. > >If that is the nature of their business, then that is the nature of their >business. The vendor runs his business the way he wants to, the customer >spends his >money the way he wants to. Capitalism. > >} I personally have been to the Czech Republic several >} times to demonstrate our systems, however, it can certainly not be >} justified in every instance. To dismiss vendors so capriciously would >} be doing a disservice to Dr. Zemek. > >No, Dr Zemek can make his own decisions. I was suggesting one course of >action for his >consideration. > >} Perhaps the company offering the >} best solution at the best price has a better method of demonstrating its >} performance than through an on-site demo. Technology these days is >} pretty impressive.Speaking as a family business owner and microscopy >vendor for >} nearly 40 > >} years, I guess I take it a bit personally when vendors are treated with >} such disrespect. > >Not disrespect, it is just the way things operate in my world. How is >'not buying >a product without a demo to see if it fits your lab's needs' disrespectful?? > >} Scientists and vendors working together rather than as >} adversaries is much more productive and serves the scientific community >} much more effectively. > >That is a very nice, general statement that no one would argue with. It >is also >180 degrees opposite of buying a product without knowing if it will >solve your >problem. > >Geoff > > > > >Geoff McAuliffe wrote: > > > >>Dear list: >> >> David Henriks wrote a very nice-sounding reply to the vendor issue >>we are all concerned about. I say nice-sounding because while it may >>be the ideal situation, it is not always a real world situation. Joe's >>troubles bear this out. >> Several years ago someone posted a querry on this listserver (or >>the Microscopy listserver, I am not sure which) about purchasing an >>expensive piece of equipment. I suggested he invite vendors to bring a >>demo to his lab to see if their equipment fit his needs. I also >>suggested that any vendor who would not supply a demo not be >>considered in his purchase plans. I got a very nasty off list reply >>from .......... David Henriks of South Bay Technologies. >> Actions speak louder than words. >> >>Geoff >> >>David Henriks wrote: >> >> >> >>>I missed the earliest part of the thread here, but from what I see it >>>seems that someone criticized a vendor on the Histonet and then was >>>reprimanded by his boss for doing so after the vendor complained. >>>Now, everyone wants to ban vendors from the Histonet. I am a vendor >>>and I belong to many listservers. End users seem to have a very >>>skewed view of what a vendor is and what motivates us. My main >>>business is not histology, although we do offer some products for the >>>industry. Yes, companies are in business to make money. No >>>question. To say that patient care is not even on their radar screen >>>is ridiculous. If the customer's need is to improve patient care, >>>then that is what a vendor is striving for. Vendors make money by >>>supplying what customers want. If you want tools for better patient >>>care, then that is what we provide. If your needs change, then our >>>products change. We respond to your needs. If, as you say, patient >>>care is not even on the vendor radar screen, then that probably means >>>that customers have not made that a high priority in what they say >>>they need. Vendors do not make money by producing ineffective >>>products that customers don't want. The best way to ensure that >>>customers get what they need and vendors produce products to meet >>>those needs is to have an open forum where these issues can be freely >>>discussed. That requires interchange between end users and vendors. >>>Yes, there should be limitations - vendors should not overtly market >>>their products on the Histonet. They should respond to postings with >>>viable solutions - even if they involve their own products. Vendors >>>should not use this as a forum to denigrate their competition. By >>>the same token, end users should not use this as a forum to air their >>>grievances about a product or a manufacturer. A single negative >>>comment about a vendor or his product could have a devastating effect >>>on their business. Perhaps your complaints are justified, but there >>>are better ways to go about having your concerns heard. Any credible >>>vendor would love to hear from you if you are having a problem with >>>one of their products. Unfortunately, most customers do not contact >>>the company. Sometimes when they do contact a company they reach the >>>wrong person and do not get a satisfactory response. I can >>>understand the frustration. However, sharing your frustration in >>>such a public forum is not right. Just as it would not be right for >>>you to criticize one of your colleagues on the Histonet. >>>There are always the few that think vendors are their enemies and >>>think that they are solely motivated by greed. The reality is that >>>vendors and end users are in this together. The people that figure >>>that out early on are generally the most successful and the one who >>>actually drive the development of new products, new techniques and >>>make significant strides in their field. To ban vendors from your >>>site would be regrettable and counterproductive. >>> >>>The listserver should be used for a free exchange of ideas and not as >>>a venue for criticism. Praise in public, criticize in private. >>> >>>Can't we all just get along? >>> >>>Best regards- >>> >>>David >>> >>> >>> >> >> > > > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: +1-949-492-2600 Toll-free in the USA: +1-800-728-2233 FAX: +1-949-492-1499 email: henriks@southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From vazquezr <@t> ohsu.edu Wed Oct 25 17:23:22 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Oct 25 17:23:51 2006 Subject: [Histonet] Re: goodbye - vendor perspective - sorry, can't help it Message-ID: Oh come on, you know that was a chew tobacco stain....:>) sorry couldn't help myself!!! robyn From AnthonyH <@t> chw.edu.au Wed Oct 25 17:50:31 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Oct 25 17:52:13 2006 Subject: [Histonet] Microwave processing for renal biopsies Message-ID: Yep, We do it routinely using the Milestone Mega TT. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chaussey, Leslie Sent: Wednesday, 25 October 2006 10:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing for renal biopsies Does anyone have experience with microwave processing specific to STAT renal biopsies? Thanks. Leslie J Chaussey Manager, Surgical Pathology Northwestern Memorial Hospital 312 / 926-9510 lchausse@nmh.org ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mtarango <@t> nvcancer.org Wed Oct 25 18:15:27 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Oct 25 18:15:46 2006 Subject: [Histonet] Vendor Perspective Message-ID: <5AEC610C1CE02945BD63A395BA763EDEC9A12C@NVCIEXCH02.NVCI.org> We're not retarded. We know that you said to share negative opinions only in private and that positive ones are okay for all to see. There were plenty of people who held high opinions of Ventana. Ventana people could chime in on this conversation anytime, it's not like we're not talking about them. When vendors come to the histonet trying to sell something and it's totally unsolicited, that's another story. If we're talking about them, I doubt anyone would have a problem with hearing from them. I'm surprised they've been so quiet. We know that they're reading this. Mark T. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Henriks Sent: Tuesday, October 24, 2006 7:39 PM To: Amos Brooks Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Vendor Perspective Amos: Everyone seems to misunderstand what I said. I never said not to share your opinion. I said to share it in private. I would support stating your negative opinions on the listserver if the entire conversation took place on the listserver. I think it is unfair for someone to toss out a negative opinion without including the background and without the vendor having the opportunity to respond. I don't think anyone wants to be inundated with all of the details of someone's individual situation, however I think it is necessary to put the comments in context. As an example, one of the listserve members made some negative personal comments about me. When I defended myself and tried to provide the complete picture I was greeted with people telling me I was "grasping at straws" and told to "shut the *&?! up". With that in mind, it seems that a vendor won't get a fair shake here even to defend his good name. It seems to me that it makes more sense to send the negative comments off line and let the people who have the real interest sort it out. Alternatively, you need to happily accept the entire thread from anyone who wants to post without castigating them both on and off line. Also, it should be noted that one hot headed remark certainly can be slanderous - particularly when made openly to a forum of thousands of potential customers, employers and colleagues. Even within the first amendment there are limits to what can be said. Although, I don't see this as so much a 1st amendment issue as I do an issue of common decency. Best regards- David Amos Brooks wrote: > David, > I have to respectfully disagree. This is a group forum, and while I > feel stifled at times about offering my experiences with products, it > would > entirely defeat the purpose of this forum if only the sunshiny glowing > references were permitted. It's just not a reflection of reality. Not > everyone will like "Product A" and they have every right to say so. > If you feel there is any basis to fight this First Ammendment case I > think you should start with the bumperstickers of car manufacturer logos > being watered down. (see: > http://www.customnames.com/images/calvin_Ford.jpg ) > (Apologies to Ford Royer, tough thread my friend) If you can get anywhere > with that then I'm sure you will see people being more cautious about > saying > anything bad about your products. They will probably all be moving to > North > Korea for more personal freedom too. > The ideal thing for the vendor to do would be to contact the person > making the complaint and see what can be done to fix the problem. > Accepting > the fact that it is entirely possible that the customer may be too far > gone > to do anything about. No harm in trying though. > Now it must be said that there is a limit to expressing a negative > opinion and simply slandreing or bashing a company. This is > unacceptable too > and in this case the vendor needs to do what they need to do to stay > alive. > Honestly though one hot headed remark does not slander make. If it really > bugs you find a customer you have a good relationship with to counter the > claim. > I have to disagree emphatically with any call to have vendors bumped > off the Histonet. As a matter of fact I think we need more, but they > need to > understand the basics of the Histonet. I have had Vendors and Sales Reps > give very good advice and have benefitted very much from their help > over the > years. But it is imperative that they keep in mind the purpose of this > forum. It is not to be used as a sales platform. If you are helpful and > answer customer's questions they will remember you. If you hunt them down > and chastize them for daring to not like your product ... they will > remember > you. > > Cheers, > Amos Brooks > > -------------------------------------------- > From: David Henriks > Subject: Re: [Histonet] Re: goodbye - vendor perspective > To: Douglas D Deltour > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <453E48AE.2050505@southbaytech.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > John should answer Jane's question. > > John should answer Jane's question directly to Jane. > > John should not post his comments to the listserver as a whole. > > -- > David Henriks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA TEL: 949-492-2600 Toll-free in the USA: 800-728-2233 FAX: 949-492-1499 email: henriks+AEA-southbaytech.com Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy. Please visit us online at www.southbaytech.com. The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling 949-492-2600 and delete this message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From abishop <@t> medlabcentral.co.nz Wed Oct 25 18:46:31 2006 From: abishop <@t> medlabcentral.co.nz (Alan Bishop) Date: Wed Oct 25 18:50:45 2006 Subject: [Histonet] talking of unsolicited emails....... Message-ID: Anyone else get the email from Patrick Levasseur, Econo-Lab [info@econo-lab.com]? They have clearly harvested emails from histonet as I got the mail to my home and work emails, both of which are subscribed to histonet. I am more than happy for vendors to be on the list as they often have solutions to things and offer help but I do think harvesting emails and putting them on a mailing list for their company is out of order. Apparently I have to email to be taken off their mailing list too! Alan Bishop Charge scientist Histology Medlab Central Palmerston North New Zealand Tel: 06 952 3135 Fax: 06 952 3199 From jqb7 <@t> cdc.gov Wed Oct 25 19:03:19 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Oct 25 19:03:25 2006 Subject: [Histonet] Waterbaths References: <20061025195140.51987.qmail@web37707.mail.mud.yahoo.com> Message-ID: I have the square Surgipath waterbath and love it. It heats very rapidly because there is no glass dish and the black background and lighting makes viewing the sections a breeze. Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Tournear Sent: Wed 10/25/2006 3:51 PM To: Histonet Cc: Subject: [Histonet] Waterbaths Hi all, I'm in the process of buying new water baths....has/is anyone using SurgiPath's? Is there any one vendor better than another? Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Oct 25 19:08:41 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Oct 25 19:08:47 2006 Subject: [Histonet] talking of unsolicited emails....... In-Reply-To: References: Message-ID: Indeed I did and it irritated me. I make a conscious effort not to purchase from companies who solicit business by stealing information off of a listserv and spamming email accounts .... so quite honestly it's their loss. >Anyone else get the email from Patrick Levasseur, Econo-Lab >[info@econo-lab.com]? They have clearly harvested emails from histonet as I >got the mail to my home and work emails, both of which are subscribed to >histonet. > >I am more than happy for vendors to be on the list as they often have >solutions to things and offer help but I do think harvesting emails and >putting them on a mailing list for their company is out of order. Apparently >I have to email to be taken off their mailing list too! > >Alan Bishop >Charge scientist >Histology >Medlab Central >Palmerston North >New Zealand > >Tel: 06 952 3135 >Fax: 06 952 3199 > -- From mtyler <@t> uctgsh1.uct.ac.za Thu Oct 26 00:31:14 2006 From: mtyler <@t> uctgsh1.uct.ac.za (Tyler) Date: Thu Oct 26 00:31:24 2006 Subject: [Histonet] thanks Message-ID: <45404822.1F91FF16@uctgsh1.uct.ac.za> To all the histonetters Thank you for your advice and help.Everyone always so willing to give their knowledge and experience. Thanks again Marilyn From d.a.faichney <@t> stir.ac.uk Thu Oct 26 04:23:46 2006 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Thu Oct 26 04:24:10 2006 Subject: [Histonet] Repetitive strain? In-Reply-To: <936BDBD9AB6ED84FB1FD25FD55DCDFB10225AA3B@uwhis-xchng4.uwhis.hosp.wisc.edu> Message-ID: <38E5B431A5228D46A9A99E0206D5788317CE57@medwin.ad.stir.ac.uk> In answer to your question Jean, I cover anything Aquaculture related, but mainly Salmon and Trout. We are a commercial fish disease diagnosis lab and provide this service worldwide to a variety of clients from multi million pound farms to a single aquarist. Our Veterinary pathologists diagnose and provide treatments for fish health. We do this in conjunction with other laboratories within the Institute such as bacteriology, virology, parasitology and Water Quality . Specifically in histology, I cut most of the organs; Eyes, gills, muscle/skin, brain, kidney, spleen, gut, liver and heart. More unusual items have been, crocodile, shark, seahorses, sea urchins, and limpets!! These usually provide me with a challenge. We also work to Good Laboratory Practice, GLP regulations which is a Quality assurance standard and is required when carrying out research trials for novel feeds, vaccines etc to assure the integrity of the study. Hope this helps Debbie -----Original Message----- From: Mitchell Jean A. [mailto:JMitchell@uwhealth.org] Sent: 25 October 2006 15:42 To: Deborah Faichney Subject: RE: [Histonet] Repetitive strain? Deborah: Looking at your place of work "Institute of Aquaculture" I am very curious as to what histology work that you do? If you don't mind sharing that with me I would appreciate it. To address your problem: I have worked in histology for close to 30 years (YIKES) and have been fortunate enough to have moved to a variety of fields over those years and have not spent more than 10 years at a time doing one particular specialty. Luckily I have not experienced more than minor neck pain and frozen hands from spending hours in the cryostat. I hope that some possibly remedies are forwarded your way. Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital and Clinics Neuromuscular Laboratory Manager Madison, WI 53792 USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deborah Faichney Sent: Wednesday, October 25, 2006 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Repetitive strain? Hello all, I have worked within Histology for 18 years and have progressively felt muscle fatigue and discomfort in the wrists, elbows and neck. I also have numbness and tingling in my left index finger. It is now being addressed by my Occupation Health department (although they are mainly used to Display screen monitoring as we are in a university setting) In the meantime, I thought I may gain some ergonomics advice or alternative methods used by those experiencing the same or similar. I believe that it is mainly manual microtomy and forceps work which are causing the problems. I remember seeing something once about a tennis ball being placed over the handwheel to give a better gripping position. Anyone else know of this? Or a better solution? Also, another person mentioned using Scissor forceps, are these any good? Any and all responses welcomed. Debbie Faichney Institute of Aquaculture Histopathology University of Stirling Stirling Scotland UK -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. From ereagan2005 <@t> aol.com Thu Oct 26 05:14:39 2006 From: ereagan2005 <@t> aol.com (ereagan2005@aol.com) Date: Thu Oct 26 05:14:53 2006 Subject: [Histonet] stupid question Message-ID: <8C8C6FEAE64789E-C24-2FA6@WEBMAIL-MB06.sysops.aol.com> hey, i know to all you vets out there this might be a stupid question but here it goes. i was on the job trained and it was ingrained into me to shut down and fumigate the cryostat after a hep or tb pt. what i was never told is why. is it a universal precaution does it effect the outcome of other tissue cut in that cryo? try not to make me feel even more stupid but i really don't know why. i do it but i just can't explain to anyone why. ALSO--------- i am looking for help i am in the Hampton roads Virginia area and i need someone, anyone with any experience level to help me in the mohs lab i work in. just so you all know i am so glad i signed up for this, i learn something new everyday! so if anyone can help me i would much appreciate it. THANK YOU Lib ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Oct 26 05:25:29 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Oct 26 05:24:50 2006 Subject: [Histonet] stupid question Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC844@wahtntex2.waht.swest.nhs.uk> hey, i know to all you vets out there this might be a stupid question but here it goes. i was on the job trained and it was ingrained into me to shut down and fumigate the cryostat after a hep or tb pt. what i was never told is why. is it a universal precaution does it effect the outcome of other tissue cut in that cryo? try not to make me feel even more stupid but i really don't know why. i do it but i just can't explain to anyone why. Hep b is a very nasty virus and TB is a very nasty bug; if you cut unfixed tissue containing either of these in a cryostat then you contaminant it, the machine, with these pathogens. Whilst it could be said that at that temperature the pathogen may be inactive, if you don't disinfect then you contaminate anything else you cut with it. So you would have to handle all sections cut in that machine the same as you handle sections cut from the original infected material, because if you don't you might die! I assume you handle unfixed sections from tissue containing these pathogens with respect as I suppose if you don't then you don't need to disinfect the cryostat but you might still die. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Thu Oct 26 05:52:49 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Oct 26 05:53:07 2006 Subject: [Histonet] prefer fixative vs formalin Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C4F@LIL.xRothGen.nhs.uk> Could one of you kind folks remind me of the content of prefer, and how the cost compares to (bought) NBF. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Sue Barnes [mailto:SBarnes@elch.org] Sent: 25 October 2006 19:50 To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prefer fixative vs formalin We use Prefer in the surgery area and only have formalin on the tissue processor. We set a delay so that the tissue is in formalin for approximately 6 hours. The time in prefer is a couple of hours unless the surgery is done on the weekend. We also use the Ventana for IHC and have had no problems with the staining. We tried to use only Prefer and had some problems with the IHC but have heard from others who use only Prefer that you have to work with them a little. There is no need to do retrieval with only Prefer. We send some specimens out for prognostic markers and they require formalin fixation, but they have had no problems with our tissues. Hope this helps -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heather Renko Sent: Monday, October 23, 2006 8:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prefer fixative vs formalin Hello Fellow Histonetters, I am trying to eliminate some of the formalin in our lab and my safety officer has suggested that we can have our outpatient facilities and surgery department send our specimens to us in prefer and then transfer to formalin in our processor. Any experience and or thoughts on this. Additionally, what is your experience with switching over completely to prefer?? We run a normal overnight processing, no microwave processing and we do H&E, special stains, and immunohistochemistry on the Ventana platform. Thank you in advance. --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Thu Oct 26 07:44:33 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Thu Oct 26 07:44:49 2006 Subject: [Histonet] talking of unsolicited emails....... In-Reply-To: References: Message-ID: <4540ADB1.6070307@ebsciences.com> Yes; I wondered how I got on that list. Though I'm a vendor, I don't send unsolicited e-mails, so I can't look at it as a manifestation of karma, either. :-) -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" You must be the change you want to see in the world. - Mahatma Gandhi Alan Bishop wrote: > Anyone else get the email from Patrick Levasseur, Econo-Lab > [info@econo-lab.com]? They have clearly harvested emails from histonet as I > got the mail to my home and work emails, both of which are subscribed to > histonet. > > I am more than happy for vendors to be on the list as they often have > solutions to things and offer help but I do think harvesting emails and > putting them on a mailing list for their company is out of order. Apparently > I have to email to be taken off their mailing list too! > > Alan Bishop > Charge scientist > Histology > Medlab Central > Palmerston North > New Zealand > > Tel: 06 952 3135 > Fax: 06 952 3199 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting <@t> geisinger.edu Thu Oct 26 08:37:06 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Oct 26 08:37:31 2006 Subject: [Histonet] Ventana help Message-ID: <454081C2020000C90000121E@GHSGWIANW5V.GEISINGER.EDU> Hi Histonetters, I'm running Ultraview detection on my Benchmark XTs. Anyone have a protocol for CD10 using Ventana's predilute? Thanks! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From RCazares <@t> schosp.org Thu Oct 26 08:43:03 2006 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Oct 26 08:43:17 2006 Subject: [Histonet] Re: Surgipath water baths Message-ID: <913FAC2B773C19488E26AE6572180FA505B27FB6@exch01.schosp.org> We use Surgipath's waterbaths and we love them. I did want to mention that they offer a double bath unit, one for water with adhesive for H&E's and one for plain DI water for immunos. We are planning to purchase 2 of these. Right now we are dumping our water several times a day depending on when the immunos are ordered. Using (+) charged slides for everything can get a little expensive. Of course, this is just my opinion. Ruth Cazares Swedish Covenant Hospital *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From lisab <@t> hoho.org Thu Oct 26 08:42:37 2006 From: lisab <@t> hoho.org (Lisa Brenner) Date: Thu Oct 26 08:43:23 2006 Subject: [Histonet] Leica Autostainer XL and Leica CV5030 glass coverslipper Message-ID: Hello all, Do any of you use the Leica autostainer XL? What are your likes/dislikes? How does it compare to Sakura? Do any of you have the Leica glass coverslipper? Likes or dislikes especially if you have it connected to Leica stainer. We also have a Thin prep imager. Do any of you use the Leica coverslipper with the glass coverslips with the imager? Does it read the slides okay and do you have any problems with cornflaking? Any feed back will be greatly appreciated. We need to make up our mind soon and the powers that be are pushing us to hurry up. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hoho.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From funderwood <@t> mcohio.org Thu Oct 26 09:25:55 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu Oct 26 09:26:25 2006 Subject: [Histonet] steamed about steamer Message-ID: Hi All, I'm attempting to use a rice steamer for HIER, but am not able to get the temperature of the retrieval solution above 85 degrees C. I was not able to find locally the Black and Decker HS2000 that has been recommended in literature. I can't believe that is the only unit that will work. Is there something I'm missing here. I'm no Wolfgang Puck, but come on it's a rice steamer! Thanks in advance, Fred From ergonomics <@t> ehs.unc.edu Thu Oct 26 09:28:18 2006 From: ergonomics <@t> ehs.unc.edu (Bertmaring, Ian (Environment Health & Safety)) Date: Thu Oct 26 09:28:29 2006 Subject: [Histonet] Repetitive strain? Message-ID: <79ACF88C02E12F419FE8ECA36001D8FA9513D0@auxsexc1.aux-services.unc.edu> All, My first post to the list...mainly because I am only on here for ideas and learning more about your profession and how ergo can be applied. So here goes for my 2 cents. Deborah, As far as Repetitive Strain goes, it's pretty rampant in your field. Unfortunately it's the nature of the beast. A couple things that work have been mentioned in previous posts, so go to the website and search the posts for ergonomic, repetitive strain, etc. -------------- A few quick solutions: padded forceps - Scissor would be better for holding or positioning things for a period of time the traditional style works similar, but it's up to you as to which is better. Look here http://www.excelta.com/ for padded forceps or make your own from hardware supplies like weather stripping. Foot controlled microtome/cryostat - If you can train yourself to use foot controlled microtomes and cryostats do! Manual ones are awkward to use (posture - reaching) and are hand intensive. Electronic microtome/cryostat - same deal but better Rotate job duties every 30 minutes if possible - split up the monotony and reduce the risk of injury Take frequent rest breaks (every 30 min for 1-2 min)- research has shown frequent rest breaks to be better for your body and increases productivity. And lastly, don't over do it. Work a standard day, not 10-12 hrs, if this is the case, look for means to get help (more staff - yes the company is in most cases saving costs at your expense, so get your managers to understand the predicament if you are working long hours). Working long hours increases your risk and is not worth ruining your body. -------------- That's about all I can think of right now, but there is more in past posting (one quoted below). Try to keep reply emails to me to a minimum, I'll be gone until November 6th. Hope this helps! Ian Ian Bertmaring, MS, AEP - Ergonomist Department of Environment, Health & Safety 1120 Estes Drive Extension, CB #1650 Chapel Hill, NC 27599 - 1650 Phone: (919) 843 - 4642; Fax: (919) 962 - 0227 ergonomics@ehs.unc.edu http://www.ehs.unc.edu/workplace_safety/ergonomics/ ------------------------------------------- One small note--I purchased a small pamphlet written by a PT a few years ago called "Hand Exercises for Knitters" which I find useful even though I knit only rarely any more. My ex-husband who has weakness on one side as the result of a stroke also likes the exercises. If anyone is interested, I'll bring the book from home and post whatever publishing information is available. Barbara Albert UCSF Medical Center San Francisco >From: Gayle Callis >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ergonomic woes with microtomy >Date: Wed, 25 Oct 2006 12:28:17 -0600 > >We built up the handle of fly wheel and coarse advance wheel with taped on >bubble wrap. A tennis ball is fairly large, but some people use bicycle >handle grips (spongy, thick) which slip over microtome handles easily. >We tried racquet balls also, but didn't like the feel although some use >them successfully. > >Also, do NOT rock the fly wheel when trimming, this causes the wrist to >bend up and down (watch yourself do this!) and is a unwanted but easily >avoidable repetitive motion. Don't say you can't learn to do this, it >takes only a few times to relearn a new motion. Jan Minshew from Leica >would agree, she is an ergonomic expert at Leica and teaches this. >Also, grasp the handles so you never bend you wrist, hold the handles >with palms facing the ends rather than grasping the handle like a >bicycler. This keeps the wrists rigid and straight for both trimming and sectioning. > >No resting arms on counter or get arm rests, use a decent chair, feet >squarely on floor or use a foot rest, and all tools within easy reach. >Replacement of waterbath so it is on an L shaped counter next to you >rather than next to microtome prevents some unneeded twisting and stretching. >Learn to exercise and stretch you hands, fingers, wrists to relieve >stresses. > >This wonderful ergonomic forceps from Surgipath and others (have holes >in >handles) are very easy to use if you find you don't like the forceps >that have opposition open/close mechanism. > >Automated microtomes are wonderful if you can buy one and certainly on >our wish list. > >Good luck >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) ------------------------------------------ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deborah Faichney Sent: Thursday, October 26, 2006 5:24 AM To: Mitchell Jean A. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Repetitive strain? In answer to your question Jean, I cover anything Aquaculture related, but mainly Salmon and Trout. We are a commercial fish disease diagnosis lab and provide this service worldwide to a variety of clients from multi million pound farms to a single aquarist. Our Veterinary pathologists diagnose and provide treatments for fish health. We do this in conjunction with other laboratories within the Institute such as bacteriology, virology, parasitology and Water Quality . Specifically in histology, I cut most of the organs; Eyes, gills, muscle/skin, brain, kidney, spleen, gut, liver and heart. More unusual items have been, crocodile, shark, seahorses, sea urchins, and limpets!! These usually provide me with a challenge. We also work to Good Laboratory Practice, GLP regulations which is a Quality assurance standard and is required when carrying out research trials for novel feeds, vaccines etc to assure the integrity of the study. Hope this helps Debbie -----Original Message----- From: Mitchell Jean A. [mailto:JMitchell@uwhealth.org] Sent: 25 October 2006 15:42 To: Deborah Faichney Subject: RE: [Histonet] Repetitive strain? Deborah: Looking at your place of work "Institute of Aquaculture" I am very curious as to what histology work that you do? If you don't mind sharing that with me I would appreciate it. To address your problem: I have worked in histology for close to 30 years (YIKES) and have been fortunate enough to have moved to a variety of fields over those years and have not spent more than 10 years at a time doing one particular specialty. Luckily I have not experienced more than minor neck pain and frozen hands from spending hours in the cryostat. I hope that some possibly remedies are forwarded your way. Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital and Clinics Neuromuscular Laboratory Manager Madison, WI 53792 USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deborah Faichney Sent: Wednesday, October 25, 2006 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Repetitive strain? Hello all, I have worked within Histology for 18 years and have progressively felt muscle fatigue and discomfort in the wrists, elbows and neck. I also have numbness and tingling in my left index finger. It is now being addressed by my Occupation Health department (although they are mainly used to Display screen monitoring as we are in a university setting) In the meantime, I thought I may gain some ergonomics advice or alternative methods used by those experiencing the same or similar. I believe that it is mainly manual microtomy and forceps work which are causing the problems. I remember seeing something once about a tennis ball being placed over the handwheel to give a better gripping position. Anyone else know of this? Or a better solution? Also, another person mentioned using Scissor forceps, are these any good? Any and all responses welcomed. Debbie Faichney Institute of Aquaculture Histopathology University of Stirling Stirling Scotland UK -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Thu Oct 26 09:47:07 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Thu Oct 26 09:47:19 2006 Subject: [Histonet] steamed about steamer. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1E90@SJMEMXMB02.stjude.sjcrh.local> We have used the Oster and Black N Decker steamers and both of them work fine. On occasion we have had to return a steamer because it did not reach temperature so maybe you just got a bad steamer. We have also found that the steamers with a dial are easier to monitor temperature than the digital ones. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Thursday, October 26, 2006 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] steamed about steamer. . Hi All, I'm attempting to use a rice steamer for HIER, but am not able to get the temperature of the retrieval solution above 85 degrees C. I was not able to find locally the Black and Decker HS2000 that has been recommended in literature. I can't believe that is the only unit that will work. Is there something I'm missing here. I'm no Wolfgang Puck, but come on it's a rice steamer! Thanks in advance, Fred _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Oct 26 10:26:03 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Oct 26 10:25:41 2006 Subject: AW: [Histonet] Ventana help In-Reply-To: <454081C2020000C90000121E@GHSGWIANW5V.GEISINGER.EDU> Message-ID: <001601c6f913$0c88e1f0$c812a8c0@dielangs.at> Hi Angela, we use it as follows: CC1 mild (30 min), incubation at 37?C for 40 min. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz Austria +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Angela Bitting Gesendet: Donnerstag, 26. Oktober 2006 15:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ventana help Hi Histonetters, I'm running Ultraview detection on my Benchmark XTs. Anyone have a protocol for CD10 using Ventana's predilute? Thanks! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Thu Oct 26 10:36:47 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Oct 26 10:37:14 2006 Subject: [Histonet] Repetitive strain? In-Reply-To: <38E5B431A5228D46A9A99E0206D5788317CE55@medwin.ad.stir.ac.uk> References: <38E5B431A5228D46A9A99E0206D5788317CE55@medwin.ad.stir.ac.uk> Message-ID: Jan Minshew wrote a good article on cumulative stress disorders for Microscopy Today, 1999, vol 7 #6, pp27-29. Available by free download at: http://www.microscopy-today.com/cgi-bin/MTWWWListingSQL.pl If the direct link doesn't work, go to http://www.microscopy-today.com and click on "cumulative table of contents", then scroll to Vol 7 #6, July 1999. Phil >Hello all, > >I have worked within Histology for 18 years and have progressively felt >muscle fatigue and discomfort in the wrists, elbows and neck. I also >have numbness and tingling in my left index finger. It is now being >addressed by my Occupation Health department (although they are mainly >used to Display screen monitoring as we are in a university setting) >In the meantime, I thought I may gain some ergonomics advice or >alternative methods used by those experiencing the same or similar. I >believe that it is mainly manual microtomy and forceps work which are >causing the problems. I remember seeing something once about a tennis >ball being placed over the handwheel to give a better gripping position. >Anyone else know of this? Or a better solution? Also, another person >mentioned using Scissor forceps, are these any good? > >Any and all responses welcomed. > >Debbie Faichney >Institute of Aquaculture >Histopathology >University of Stirling >Stirling >Scotland >UK -- Philip Oshel Technical Editor, Microscopy Today Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 fax: (989) 774-3462 From cmiller <@t> physlab.com Thu Oct 26 11:19:38 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 26 11:19:48 2006 Subject: [Histonet] steamed about steamer In-Reply-To: Message-ID: <001201c6f91a$88ff5aa0$db01a8c0@plab.local> This is kind off the subject but, I have a Black and Decker Rice steamer we have used for 5 or 6 years and now most of my retrievals aren't working. Mostly my Pins,TTF and E-Cadherin. The troubleshooting I have done recently points to a worn out steamer. Anyone else have this problem?? Let me Know what you think. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Thursday, October 26, 2006 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] steamed about steamer Hi All, I'm attempting to use a rice steamer for HIER, but am not able to get the temperature of the retrieval solution above 85 degrees C. I was not able to find locally the Black and Decker HS2000 that has been recommended in literature. I can't believe that is the only unit that will work. Is there something I'm missing here. I'm no Wolfgang Puck, but come on it's a rice steamer! Thanks in advance, Fred _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu Oct 26 11:20:46 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Oct 26 11:21:03 2006 Subject: [Histonet] BK VIRUS, C4d ANTIBODY Message-ID: Currently we use two RUO antibodies in our laboratory. They are antibodies for BK virus and C4d. Does anybody know of and IVD or ASR antibodies for BK or C4d. My understanding is that if there are we need to be using them instead of the current RUO antibodies. I do know about the other requirements of using ASR and RUO antibodies such as the disclaimer statement, validation, and failed searches. Thank you. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From cwscouten <@t> myneurolab.com Thu Oct 26 11:27:54 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Thu Oct 26 11:27:55 2006 Subject: [Histonet] Flash Freezing Brain Message-ID: <5784D843593D874C93E9BADCB87342AB01307766@tpiserver03.Coretech-holdings.com> The holes are commonly referred to as "Swiss Cheese Artifact" and are due to freezing too slowly. Try freezing the brains directly by immersion in isopentane, no foil, no OCT. You may be using so much OCT it is slowing down the rate of heat transfer. Then put just a little OCT on the pedastal as glue, put the block of tissue on, and freeze again. How long do you store the tissue? Eventually, the ice reformats, and you get Swiss Cheese no matter how quickly you froze it, or no matter how cold you stored it. It depends on a lot of variables, but I would give you a month. I have heard stories of a year. See the article in the following link: http://www.myneurolab.com/global/Manuals/Tips%20and%20Techniques%20Freez ing%20Artifact.pdf Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of acjanes@bu.edu Sent: Monday, October 23, 2006 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flash Freezing Brain I have a question about flash freezing rodent brains, as sometimes I see little holes destroying my tissue and I cannot determine the cause. Normally I perfuse mice with 4% para, post fix for 1-4 hours with para at room temp then put in 30% sucrose until the brain drops (At 4C). After this I make an foil mold which I fill with OCT and put my brain inside. I then slowly lower the foil/brain mold into a bath containing isopentane and dry ice just until everything freezes. I then store the tissue at -20 until ready to cut. Normally this works, but occasionally I loose tissue to the holes. I am now going to be starting a project using rats and I want to make sure all the tissue stays intact. Does anyone have suggestions? We need the tissue to run ICC for c-fos the brains will be cut at 40 microns using a cryostat. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtcrhb <@t> umr.edu Thu Oct 26 11:40:18 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Thu Oct 26 11:41:30 2006 Subject: [Histonet] decalcifying with Jenkin's fluid References: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D78@UMR-CMAIL1.umr.edu> Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D79@UMR-CMAIL1.umr.edu> I need to know about how long I should leave a speciman in Jenkin's fluid to become fully decalcified. I am sure it depends on the size of the speciman but if someone can tell me how long they leave their specimens in and what size they are I can probably figure out the length at which I should leave mine in. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 From ASenn <@t> mercy.pmhs.org Thu Oct 26 11:55:35 2006 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Thu Oct 26 11:55:53 2006 Subject: [Histonet] cryostat Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A66@pmhs2kxch03> LIB-----I was also trained to disinfect the cryostat after a hep or TB patient, but we don't shut it down/defrost it. It's cleaned after every (most times) frozen with 100% alcohol & at the end of the day it's disinfected with a spray. Except with the hep or TB cases--then it's done right after the case is finished. ALAN----Yep, I got that email also from Econo-Lab. That thief. Hope you all have a good day... Amy From LRaff <@t> lab.uropartners.com Thu Oct 26 11:55:50 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Oct 26 11:56:03 2006 Subject: [Histonet] Cytologists with FISH experience Message-ID: <5DA1CA5D0B98A84985B545A24423B822019A27@UPLAB01.uplab.local> Histonetters: If any of you have friends across the hall with people in cytology or molecular biology with FISH experience who might be interested in spending some time in the Chicago area, please pass on my contact information below. Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From pruegg <@t> ihctech.net Thu Oct 26 12:07:55 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Oct 26 12:07:57 2006 Subject: [Histonet] NSH 2007 Message-ID: <200610261707.k9QH7WJu014924@pro12.abac.com> For all ya'll thinking about coming to the NSH S/C in Denver at this time next year don't let our snow storm today scare you off, it is supposed to be in the 60's tomorrow. Dress in layers is what we say around here. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net From rjbuesa <@t> yahoo.com Thu Oct 26 12:30:14 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 26 12:30:21 2006 Subject: [Histonet] steamed about steamer In-Reply-To: <001201c6f91a$88ff5aa0$db01a8c0@plab.local> Message-ID: <20061026173014.55130.qmail@web61220.mail.yahoo.com> 3 questions Cheri: have you determined the temperature now obtained with your steamer? Did you determine the operating temperature when new? If so, is now lower than when new? Answering those question will permit you to evaluate the performance of your steamer and decide if you need a new one. Ren? J. Cheri Miller wrote: This is kind off the subject but, I have a Black and Decker Rice steamer we have used for 5 or 6 years and now most of my retrievals aren't working. Mostly my Pins,TTF and E-Cadherin. The troubleshooting I have done recently points to a worn out steamer. Anyone else have this problem?? Let me Know what you think. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Thursday, October 26, 2006 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] steamed about steamer Hi All, I'm attempting to use a rice steamer for HIER, but am not able to get the temperature of the retrieval solution above 85 degrees C. I was not able to find locally the Black and Decker HS2000 that has been recommended in literature. I can't believe that is the only unit that will work. Is there something I'm missing here. I'm no Wolfgang Puck, but come on it's a rice steamer! Thanks in advance, Fred _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From rjbuesa <@t> yahoo.com Thu Oct 26 12:34:08 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 26 12:34:15 2006 Subject: [Histonet] decalcifying with Jenkin's fluid In-Reply-To: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D79@UMR-CMAIL1.umr.edu> Message-ID: <20061026173408.56103.qmail@web61220.mail.yahoo.com> John: Decalcifying a specimen is something particular to that specimen. How long takes to decalcify another specimen I don't think it will help you much, you will need anyway to test your specimen for the end point (with whatever procedure you chose to do so). Ren? J. "Campbell, John Thomas (UMR-Student)" wrote: I need to know about how long I should leave a speciman in Jenkin's fluid to become fully decalcified. I am sure it depends on the size of the speciman but if someone can tell me how long they leave their specimens in and what size they are I can probably figure out the length at which I should leave mine in. Thanks John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-6904 Cell phone 573-694-0596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From pwg1 <@t> cdc.gov Thu Oct 26 12:25:06 2006 From: pwg1 <@t> cdc.gov (Greer, Patricia (CDC/CCID/NCZVED)) Date: Thu Oct 26 12:49:00 2006 Subject: [Histonet] steamed about steamer Message-ID: We have been using the Black and Decker rice steamer for 5 or 6 years as well. We first heat preheat the steamer until the upper part is completely filled with steam, then heat the retrieval solution in the microwave to just boiling (ours takes about 1 minute 45 seconds. Transfer the heated solution to the steamer, add the deparaffinized slides, and set the timer for 15 minutes. After steaming the slides for the 15 minutes, rinse in RT tap water and you are ready to do your immuno. Pat Greer Infectious Disease Pathology Activity Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 26, 2006 12:20 PM To: 'Fred Underwood'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] steamed about steamer This is kind off the subject but, I have a Black and Decker Rice steamer we have used for 5 or 6 years and now most of my retrievals aren't working. Mostly my Pins,TTF and E-Cadherin. The troubleshooting I have done recently points to a worn out steamer. Anyone else have this problem?? Let me Know what you think. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Thursday, October 26, 2006 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] steamed about steamer Hi All, I'm attempting to use a rice steamer for HIER, but am not able to get the temperature of the retrieval solution above 85 degrees C. I was not able to find locally the Black and Decker HS2000 that has been recommended in literature. I can't believe that is the only unit that will work. Is there something I'm missing here. I'm no Wolfgang Puck, but come on it's a rice steamer! Thanks in advance, Fred _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Thu Oct 26 13:54:38 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Oct 26 13:54:45 2006 Subject: [Histonet] re: Water Bath....Thanks Message-ID: <20061026185438.13232.qmail@web37701.mail.mud.yahoo.com> Thanks everyone for the input on the water baths.....I'll probably go with SurgiPath... Kim HT (ASCP), QIHC (ASCP) ~~~Don't be afraid your life will end, be afraid it will never begin~~~ --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From wia2005 <@t> med.cornell.edu Thu Oct 26 14:17:34 2006 From: wia2005 <@t> med.cornell.edu (William Ares) Date: Thu Oct 26 14:17:45 2006 Subject: [Histonet] virbratome sections and staining Message-ID: <7564dff22b86.4540d18e@med.cornell.edu> I have recently started sectioning by vibratome and had some questions regarding staining, particularly H&E (hematoxylin and eosin). After sectioning, I am plating the sections on glass slides and allowing the buffer to evaporate. When reinserting the slides into buffer for H&E staining the sections fall off. If anyone else has done this, what methods do you use to make sure you do not lose your sections. Thanks. William Ares Labratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University wia2005@med.cornell.edu From dfinkelstein <@t> mhri.edu.au Thu Oct 26 17:18:06 2006 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Thu Oct 26 17:19:05 2006 Subject: [Histonet] Flash Freezing Brain Message-ID: <000901c6f94c$9d46a040$0200a8c0@davidfink> Dear acjanes@bu.edu, Your poor brains!! ;) Holes in frozen tissue = freeze fracture. Never store brains at -20 only at -80c. I agree with the other comments about how to freeze onto the chuck with Oct. For long term storage of fixed brain (remember the brain will continue to slowly fix in the freezer, so the time depends on the antigen). 1) place brain on small piece of foil or piece of plastic weigh boat. 2) place a separate piece of foil with label on dry ice. 3) lower the brain into the vapours of the liquid nitrogen just\on or above the liquid level. Use long plastic forceps. You don't wont to freeze it too long or too quickly or it will crack. 4) quickly transfer the brain onto the foil in dry ice. Do not let the brain warm up by touching it with your fingers. 5) drop foiled brain in a plastic bag, place immediately onto dry ice and then -80 freezer. Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA Mobile: 0409171227 Tel: +61 (03) 9388 1633 Fax: +61 (03) 9387 5061 dfinkelstein@mhri.edu.au [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of acjanes@bu.edu Sent: Monday, October 23, 2006 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flash Freezing Brain I have a question about flash freezing rodent brains, as sometimes I see little holes destroying my tissue and I cannot determine the cause. Normally I perfuse mice with 4% para, post fix for 1-4 hours with para at room temp then put in 30% sucrose until the brain drops (At 4C). After this I make an foil mold which I fill with OCT and put my brain inside. I then slowly lower the foil/brain mold into a bath containing isopentane and dry ice just until everything freezes. I then store the tissue at -20 until ready to cut. Normally this works, but occasionally I loose tissue to the holes. I am now going to be starting a project using rats and I want to make sure all the tissue stays intact. Does anyone have suggestions? We need the tissue to run ICC for c-fos the brains will be cut at 40 microns using a cryostat. From RSRICHMOND <@t> aol.com Thu Oct 26 18:00:43 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Oct 26 18:00:51 2006 Subject: [Histonet] Re: nuclear fast red Message-ID: <482.59eef737.3272981b@aol.com> I've never seen nuclear fast red - or hematoxylin - used for this purpose. The strong eosin sounds like a great idea. Somebody mentioned Mercurochrome (merbromin), a dye chemically related to eosin but containing 26% mercury. I've seen it used to mark small specimens. Mercurochrome used to be daubed on small cuts on small children by anxious mothers. The FDA banned it in 1999, and it's no longer available anywhere. Several years ago I posted a note on the subject on Histonet (Google mercurochrome richmond to find it), and this post resulted in my becoming THE authority on Mercurochrome for the entire Web. About once a month I get an e-mail from somebody railing against the US government for interfering with the freedom of American citizens to expose their children to potentially toxic levels of mercury. One fellow wanted Mercurochrome to surreptitiously dye stray yellow feathers on red canaries. It seems that his father was a major league canary breeder, and had run out of his supply. In replying I asked him if he knew that Martha Stewart was seriously into canaries, even resorting to shoveling her own canary poop now that she's down to her last half billion. He replied that his father was THE Official Purveyor of Canaries to said domestic goddess. You read it on Histonet. And no, I don't know where to get Mercurochrome. Bob Richmond Samurai Pathologist Knoxville TN From sjchtascp <@t> yahoo.com Thu Oct 26 18:29:05 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Oct 26 18:29:10 2006 Subject: [Histonet] temporary, contract, per diem work Message-ID: <20061026232905.69632.qmail@web38209.mail.mud.yahoo.com> I'm researching the need for histology work on a temporary, contract or per diem basis in the southern Wisconsin northern Illinois region. Steven Coakley HT(ASCP --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From amosbrooks <@t> gmail.com Thu Oct 26 19:06:10 2006 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Oct 26 19:06:18 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <582736990610261706n31224adcs56e770fab42c16e4@mail.gmail.com> Hi, I have only used 2 brands of steamer so far but there's no reason another can't work as well. Certainly steamers can wear out as one user has pointed out. I have seen this, the heating element stops getting the temperature high enough. As this is a new steamer that shouldn't be the problem. It's possible that the steaming rack is too far from the element leaving the steam enough time to cooldown before it reaches the slides. That is pretty much a design flaw if that is the case. If so there's nothing else for it but to replace it. Try to get something compact that allows enough space for whatever container you are using to hold the slide rack. It is possible that the vents are allowing the steam out too quickly. That could prevent the steam from heating the buffer up enough. If so you could cover some of the holes (Try duct tape at first, you can figure out something more permanent if it works. To make sure the buffer gets up to the right temperature, I have holes drilled in the top of my steamers just big enough to fit a thermometer. This way I know the buffer is hot enough to put the slides in and when the buffer gets back up to the right temperature after the slides are added. This is great because you can directly monitor the temperature of the solution in real time thruought the retrieval process. (Try that in a microwave or pressure cooker) In response to the gentleman that was having trouble with E-Cadherin, we have found an extra 10 min. produces a stronger antibody reaction. This works for ER & PR too. I am sure we'll find more if we actually get time to test more antibodies. Good Luck Amos Brooks From mbmphoto <@t> gmail.com Thu Oct 26 22:37:15 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Oct 26 22:36:01 2006 Subject: [Histonet] plastic mailer or boxes to store 50mmx75mm slides Message-ID: Hello, Can anyone tell me where I can purchase plastic mailer that can carry 5 slides or small plastic slides boxes that hold 4 to 12 glass slides measuring 50mmx75mm in size? I know that both products are available for the standard size glass slides, but I'm looking for something much bigger to store slides in -80C freezer. Please any information anyone can provide will be greatly appreciated. I look forward to hearing from you. Yours Maria Bartola Mejia UCSF Department of Neurosurgery San Francisco, CA 94103 From Terry.Marshall <@t> rothgen.nhs.uk Fri Oct 27 04:40:03 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Oct 27 04:42:44 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C59@LIL.xRothGen.nhs.uk> I am intrigued by this discussion. I have been using rice steamers for 42 years. I use them to cook rice, oddly enough:-) They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: 27 October 2006 01:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamed About Steamer Hi, I have only used 2 brands of steamer so far but there's no reason another can't work as well. Certainly steamers can wear out as one user has pointed out. I have seen this, the heating element stops getting the temperature high enough. As this is a new steamer that shouldn't be the problem. It's possible that the steaming rack is too far from the element leaving the steam enough time to cooldown before it reaches the slides. That is pretty much a design flaw if that is the case. If so there's nothing else for it but to replace it. Try to get something compact that allows enough space for whatever container you are using to hold the slide rack. It is possible that the vents are allowing the steam out too quickly. That could prevent the steam from heating the buffer up enough. If so you could cover some of the holes (Try duct tape at first, you can figure out something more permanent if it works. To make sure the buffer gets up to the right temperature, I have holes drilled in the top of my steamers just big enough to fit a thermometer. This way I know the buffer is hot enough to put the slides in and when the buffer gets back up to the right temperature after the slides are added. This is great because you can directly monitor the temperature of the solution in real time thruought the retrieval process. (Try that in a microwave or pressure cooker) In response to the gentleman that was having trouble with E-Cadherin, we have found an extra 10 min. produces a stronger antibody reaction. This works for ER & PR too. I am sure we'll find more if we actually get time to test more antibodies. Good Luck Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 27 04:49:57 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 27 04:49:08 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC857@wahtntex2.waht.swest.nhs.uk> They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Terry Marshall How would you know? If it boiled and took a bit longer or if the steam wasn't as 'hot', you steam it until it's done, don't you? Cooks rarely time they react to the end point, don't they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Fri Oct 27 05:05:22 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Oct 27 05:05:00 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C5C@LIL.xRothGen.nhs.uk> Kemlo, I don't get it. You put water in. It boils. It produces steam within a closed chamber. How much variability in temperature could you *possibly* get in that scenario (No smart arse comments about the top of Everest)? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 27 October 2006 10:50 To: Marshall Terry Dr, Consultant Histopathologist; Amos Brooks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Steamed About Steamer They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Terry Marshall How would you know? If it boiled and took a bit longer or if the steam wasn't as 'hot', you steam it until it's done, don't you? Cooks rarely time they react to the end point, don't they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 27 05:12:19 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 27 05:11:28 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC858@wahtntex2.waht.swest.nhs.uk> It boils more slowly, or gets to the boiling point at a slower rate. That's OK if you are steaming rice cos you wait till it's cooked; but if you time something and it takes longer to boil, you don't boil it as long. But you're not a Scientist like as what I am. Why would you be carrying out ICC on the top of Everest? Do they have a Lab up there? Why? Do they do a lot of frozen sections? Does rice taste better or worse steamed at the top of Everest? Can you steam rice at the bottom of the Sea cos water boils at a higher temperature? Um.... You'd have to boil the Sea wouldn't you? That's silly. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Fri Oct 27 05:26:34 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Oct 27 05:27:40 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C5D@LIL.xRothGen.nhs.uk> " but if you time something and it takes longer to boil, you don't boil it as long." That is a non-sequitur. (actually, when I steam eggs in the rice cooker, I time it from when the lid starts to rattle). "But you're not a Scientist like as what I am." Should read "You're not a scientist. I am." You might get the Nobel prize for science, but not for literature:-) "Do they have a Lab up there?" No, but the next mountain along has a veterinary lab. They call it K9. "That's silly." Well, we can agree there. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 27 October 2006 11:12 To: Marshall Terry Dr, Consultant Histopathologist; Amos Brooks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Steamed About Steamer It boils more slowly, or gets to the boiling point at a slower rate. That's OK if you are steaming rice cos you wait till it's cooked; but if you time something and it takes longer to boil, you don't boil it as long. But you're not a Scientist like as what I am. Why would you be carrying out ICC on the top of Everest? Do they have a Lab up there? Why? Do they do a lot of frozen sections? Does rice taste better or worse steamed at the top of Everest? Can you steam rice at the bottom of the Sea cos water boils at a higher temperature? Um.... You'd have to boil the Sea wouldn't you? But you're not a Scientist like as what I am.That's silly. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 27 05:39:19 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 27 05:38:29 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC859@wahtntex2.waht.swest.nhs.uk> " but if you time something and it takes longer to boil, you don't boil it as long." That is a non-sequitur. (actually, when I steam eggs in the rice cooker, I time it from when the lid starts to rattle). What's that? You know what I mean.... If it takes longer to boil...... I give up. "But you're not a Scientist like as what I am." Should read "You're not a scientist. I am." You might get the Nobel prize for science, but not for literature:-) That was in Cockney!!! Punctuation Terry, should be a colon not a smiley!!! "Do they have a Lab up there?" No, but the next mountain along has a veterinary lab. They call it K9. Very funny "That's silly." Well, we can agree there. Yes we do Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From lisab <@t> hoho.org Fri Oct 27 06:56:25 2006 From: lisab <@t> hoho.org (Lisa Brenner) Date: Fri Oct 27 06:57:12 2006 Subject: [Histonet] xylene resistant labels Message-ID: Hello everyone, What are you using for xylene (reagent) resistant bar coded labels? Like I said before we are implementing a new IS system and need to make these decisions now. Any input would be greatly appreciated. I also still need feed back on glass coverslipped slides being read by the thin prep imager. We are looking at the Leica XL stainer and glass coverslipper. Thank you and TGIF. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hoho.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From oshel1pe <@t> cmich.edu Fri Oct 27 07:13:48 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Oct 27 07:14:07 2006 Subject: [Histonet] Steamed About Steamer In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBFAFC858@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBFAFC858@wahtntex2.waht.swest.nhs.uk> Message-ID: This being Friday and all ... Yeah, I can see doing ICC on top of Everest. Geese and other birds fly past Up There, and they're undoubtly carrying Avian Flu and Spotted Splunge Pox, so they need to be netted and tested. Nor would one have to boil the sea to steam rice in the ocean. Just use a strong enough pressure vessel so the internal pressure can be maintained at one atmosphere, add water and rice, and stick it into a 350 deg C black smoker. Rice pilaf ala vente. Phil I know, it's a bad, inaccurate bilingual pun, but I'm only now on my second cup of coffee ... >Why would you be carrying out ICC on the top of Everest? Do they have a >Lab up there? Why? Do they do a lot of frozen sections? Does rice taste >better or worse steamed at the top of Everest? Can you steam rice at the >bottom of the Sea cos water boils at a higher temperature? Um.... You'd >have to boil the Sea wouldn't you? That's silly. > > > >Kemlo Rogerson > >Pathology Manager > >Ext 3311 > >DD 01934 647057 > >Mob 07749 754194 > >Pager 07659 597107 > >E-Mail: kemlo.rogerson@nhs.net -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From funderwood <@t> mcohio.org Fri Oct 27 07:22:23 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Oct 27 07:22:52 2006 Subject: [Histonet] Steamed About Steamer Message-ID: I'm happy to see that this has become a "Nan" issue. >>> "Marshall Terry Dr,Consultant Histopathologist" 10/27/2006 5:40 AM >>> I am intrigued by this discussion. I have been using rice steamers for 42 years. I use them to cook rice, oddly enough:-) They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: 27 October 2006 01:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamed About Steamer Hi, I have only used 2 brands of steamer so far but there's no reason another can't work as well. Certainly steamers can wear out as one user has pointed out. I have seen this, the heating element stops getting the temperature high enough. As this is a new steamer that shouldn't be the problem. It's possible that the steaming rack is too far from the element leaving the steam enough time to cooldown before it reaches the slides. That is pretty much a design flaw if that is the case. If so there's nothing else for it but to replace it. Try to get something compact that allows enough space for whatever container you are using to hold the slide rack. It is possible that the vents are allowing the steam out too quickly. That could prevent the steam from heating the buffer up enough. If so you could cover some of the holes (Try duct tape at first, you can figure out something more permanent if it works. To make sure the buffer gets up to the right temperature, I have holes drilled in the top of my steamers just big enough to fit a thermometer. This way I know the buffer is hot enough to put the slides in and when the buffer gets back up to the right temperature after the slides are added. This is great because you can directly monitor the temperature of the solution in real time thruought the retrieval process. (Try that in a microwave or pressure cooker) In response to the gentleman that was having trouble with E-Cadherin, we have found an extra 10 min. produces a stronger antibody reaction. This works for ER & PR too. I am sure we'll find more if we actually get time to test more antibodies. Good Luck Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 27 07:37:46 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 27 07:37:57 2006 Subject: [Histonet] Steamed About Steamer In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBFAFC857@wahtntex2.waht.swest.nhs.uk> Message-ID: <20061027123746.10660.qmail@web61218.mail.yahoo.com> If it gets gradually pitted by the salt until it holes, how do you hold the water? If you lose water you will not be able to cook the rice until softness! Ren? J. Kemlo Rogerson wrote: They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Terry Marshall How would you know? If it boiled and took a bit longer or if the steam wasn't as 'hot', you steam it until it's done, don't you? Cooks rarely time they react to the end point, don't they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From Kathy.Johnston <@t> CLS.ab.ca Fri Oct 27 07:51:06 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Fri Oct 27 07:56:44 2006 Subject: [Histonet] Re: nuclear fast red In-Reply-To: <482.59eef737.3272981b@aol.com> Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE01365450@mail1.calgary.com> We ordered our last bottle from Sigma Catalogue # M7011 (labelled as Mercury dibromofluorescein disodium salt), but couldn't tell you if they still supply it. We are also trying to get rid of the Mercurochrome in our lab, and did try out the hematoxylin idea here. Be darned if it didn't work! Both of our commercial prepared Harris and Gill III hematoxylin remained through processing identifying our cut side down. Kathy Johnston Tech II Special Stains Anatomic Pathology Calgary Laboratory Services #9 - 3535 Research Road NW Calgary, AB. 403-770-3572 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of RSRICHMOND@aol.com Sent: October 26, 2006 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: nuclear fast red I've never seen nuclear fast red - or hematoxylin - used for this purpose. The strong eosin sounds like a great idea. Somebody mentioned Mercurochrome (merbromin), a dye chemically related to eosin but containing 26% mercury. I've seen it used to mark small specimens. Mercurochrome used to be daubed on small cuts on small children by anxious mothers. The FDA banned it in 1999, and it's no longer available anywhere. Several years ago I posted a note on the subject on Histonet (Google mercurochrome richmond to find it), and this post resulted in my becoming THE authority on Mercurochrome for the entire Web. About once a month I get an e-mail from somebody railing against the US government for interfering with the freedom of American citizens to expose their children to potentially toxic levels of mercury. One fellow wanted Mercurochrome to surreptitiously dye stray yellow feathers on red canaries. It seems that his father was a major league canary breeder, and had run out of his supply. In replying I asked him if he knew that Martha Stewart was seriously into canaries, even resorting to shoveling her own canary poop now that she's down to her last half billion. He replied that his father was THE Official Purveyor of Canaries to said domestic goddess. You read it on Histonet. And no, I don't know where to get Mercurochrome. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Fri Oct 27 08:14:24 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Oct 27 08:17:46 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C60@LIL.xRothGen.nhs.uk> When it holes is when you get a new one! Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 27 October 2006 13:38 To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Amos Brooks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Steamed About Steamer If it gets gradually pitted by the salt until it holes, how do you hold the water? If you lose water you will not be able to cook the rice until softness! Ren? J. Kemlo Rogerson wrote: They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Terry Marshall How would you know? If it boiled and took a bit longer or if the steam wasn't as 'hot', you steam it until it's done, don't you? Cooks rarely time they react to the end point, don't they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Fri Oct 27 08:19:06 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 27 08:19:14 2006 Subject: [Histonet] Steamed About Steamer In-Reply-To: <63984BC3A63FF542AF6EF0A237F38F4D7E4C60@LIL.xRothGen.nhs.uk> Message-ID: <20061027131906.27079.qmail@web61222.mail.yahoo.com> Oh, I see! Makes sense! Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: When it holes is when you get a new one! Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 27 October 2006 13:38 To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Amos Brooks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Steamed About Steamer If it gets gradually pitted by the salt until it holes, how do you hold the water? If you lose water you will not be able to cook the rice until softness! Ren? J. Kemlo Rogerson wrote: They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Terry Marshall How would you know? If it boiled and took a bit longer or if the steam wasn't as 'hot', you steam it until it's done, don't you? Cooks rarely time they react to the end point, don't they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Creativity is a type of learning process where the teacher and pupil are located in the same individual. --Arthur Koestler This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From Terry.Marshall <@t> rothgen.nhs.uk Fri Oct 27 08:23:33 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Oct 27 08:27:43 2006 Subject: [Histonet] Steamed About Steamer Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C61@LIL.xRothGen.nhs.uk> By one of those odd coincidences, the next e-mail I received after this one was from my wife who is house sitting whilst her son and family are away, because they have the builders in. It included inter alia: "The rain stopped at midday and it is a lovely afternoon. The roofers are steaming nicely!" Shall I ask her to find out the temperature of boiling water on the roof, and how long they took to get to "steaming"? Have a good weekend everyone. PS Wonder if there are any rice cooker firms listening - I feel a pit>hole coming on. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Fred Underwood [mailto:funderwood@mcohio.org] Sent: 27 October 2006 13:22 To: amosbrooks@gmail.com; histonet@lists.utsouthwestern.edu; Marshall Terry Dr, Consultant Histopathologist Subject: RE: [Histonet] Steamed About Steamer I'm happy to see that this has become a "Nan" issue. >>> "Marshall Terry Dr,Consultant Histopathologist" 10/27/2006 5:40 AM >>> I am intrigued by this discussion. I have been using rice steamers for 42 years. I use them to cook rice, oddly enough:-) They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: 27 October 2006 01:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamed About Steamer Hi, I have only used 2 brands of steamer so far but there's no reason another can't work as well. Certainly steamers can wear out as one user has pointed out. I have seen this, the heating element stops getting the temperature high enough. As this is a new steamer that shouldn't be the problem. It's possible that the steaming rack is too far from the element leaving the steam enough time to cooldown before it reaches the slides. That is pretty much a design flaw if that is the case. If so there's nothing else for it but to replace it. Try to get something compact that allows enough space for whatever container you are using to hold the slide rack. It is possible that the vents are allowing the steam out too quickly. That could prevent the steam from heating the buffer up enough. If so you could cover some of the holes (Try duct tape at first, you can figure out something more permanent if it works. To make sure the buffer gets up to the right temperature, I have holes drilled in the top of my steamers just big enough to fit a thermometer. This way I know the buffer is hot enough to put the slides in and when the buffer gets back up to the right temperature after the slides are added. This is great because you can directly monitor the temperature of the solution in real time thruought the retrieval process. (Try that in a microwave or pressure cooker) In response to the gentleman that was having trouble with E-Cadherin, we have found an extra 10 min. produces a stronger antibody reaction. This works for ER & PR too. I am sure we'll find more if we actually get time to test more antibodies. Good Luck Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Oct 27 08:39:15 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Oct 27 08:38:53 2006 Subject: [Histonet] talking of unsolicited emails....... In-Reply-To: References: Message-ID: <45420C03.9010607@umdnj.edu> I got it too and I also avoid dealing with spammers. Geoff Andrea T. Hooper wrote: > Indeed I did and it irritated me. I make a conscious effort not to > purchase from companies who solicit business by stealing information > off of a listserv and spamming email accounts .... so quite honestly > it's their loss. > > >> Anyone else get the email from Patrick Levasseur, Econo-Lab >> [info@econo-lab.com]? They have clearly harvested emails from >> histonet as I >> got the mail to my home and work emails, both of which are subscribed to >> histonet. >> >> I am more than happy for vendors to be on the list as they often have >> solutions to things and offer help but I do think harvesting emails and >> putting them on a mailing list for their company is out of order. >> Apparently >> I have to email to be taken off their mailing list too! >> >> Alan Bishop >> Charge scientist >> Histology >> Medlab Central >> Palmerston North >> New Zealand >> >> Tel: 06 952 3135 >> Fax: 06 952 3199 >> > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Fri Oct 27 08:49:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 27 08:49:40 2006 Subject: [Histonet] =?iso-8859-1?q?Fwd=3A_In_Argentina=2C_Pathologists_Do?= =?iso-8859-1?q?n=E2=80=99t_Operate_Clinical_Laboratories?= Message-ID: <20061027134928.79568.qmail@web61215.mail.yahoo.com> Dear Colleagues: I just received this very interesting report that I want to share will all. Ren? J. DARK DAILY wrote: From: "DARK DAILY" To: rjbuesa@yahoo.com Subject: In Argentina, Pathologists Don???t Operate Clinical Laboratories Date: Fri, 27 Oct 2006 13:30:30 +0000 If you are having trouble viewing this email or any of the pages that follow, please open a new browser window and visit: http://www.darkdaily.com/daily/daily_email10.27.06.htm Add the Dark Daily to your address book and never miss an email! Simply click and save the address, Dark_Daily@mail.vresp.com Like our emails? Forward this Email to a friend! Home | Archive | About | Join-- In Argentina, Pathologists Don't Operate Clinical Laboratories And Patients Deliver Their Own Lab Test Reports to Their Doctors DATELINE?BUENOS AIRES, ARGENTINA: Believe it or not, Argentina is a country where pathologists are not associated with clinical laboratory testing! I am Argentina this week to speak to a group of laboratory owners, speak to health insurance executives, and visit clinical laboratories. My hosts on this trip are Sysmex Corporation and Roche Diagnostics Argentina. The most startling thing that I learned is that pathologists do not own clinical laboratories in Argentina, nor do they manage clinical laboratories in hospitals. That responsibility is handled by clinical biochemists. Further, interaction between anatomic pathologists and clinical laboratories is relatively limited. This is a much different situation than the common clinical models and business arrangements used in the United States, Canada, and similar nations. Another interesting difference is that doctors give their patients the laboratory test requisition. Patients then show up at the laboratory of their choice, where specimens are collected. When the results are ready, the laboratory contacts the patient, who comes by the laboratory or patient service center, picks up a paper copy of his or her test report, and then personally delivers the lab test report to his or her physician. This arrangement is counterintuitive to the American system. On the other hand, it does provide a powerful example that patients can reliably play a role this aspect of their healthcare. Competition is intense among private laboratories in Buenos Aires, Argentina. There are private health insurance companies. Labs that bid for provider status in these contracts tend to keep the prices for lab testing at unprofitably low levels. Another factor in the financial struggles of the entire health system in Argentina was the devaluation of the Argentinian Peso in 2001. The purchasing power of the Peso fell by 66% overnight! This has made the purchase of laboratory testing instruments, reagents, and other laboratory consumables prohibitively expensive for labs in Argentina. The effects of this devaluation are still dragging down the finances of this countries' lab industry. During this week, I gained many useful insights about laboratory management, different business models for laboratory testing, and the opportunities for laboratories in the United States to benefit from some of the experience provided by the laboratory testing marketplace in Argentina. These are examples of how innovations by laboratories in one country can be adopted by labs in other countries. I hope to share some of the more powerful innovations with you in coming weeks. Reporting from Buenos Aires, your faithful Editor, Robert L. Michel (E-mail Robert at rmichel@darkdaily.com or schristensen@darkdaily.com.) --------------------------------- Related Products: Executive War College 2007 taking place in Miami, Florida May 10-11, 2007. Topics Archive of DARK Daily The Dark Report The Executive War College Consumer-Directed Health Plans White Paper Dark Daily Explained About Sylvia Christensen About Robert Michel --------------------------------- You are receiving this Email Alert because you are subscribed to DARK DAILY. If you wish to Unsubscribe --------------------------------- DARK DAILY 21806 Briarcliff Dr Spicewood, Texas 78669 Read the VerticalResponse marketing policy. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From cdemarinis <@t> SARATOGACARE.ORG Fri Oct 27 09:14:06 2006 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Fri Oct 27 09:14:51 2006 Subject: [Histonet] 88331 and 88332 Message-ID: There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. From MVaughan4 <@t> ucok.edu Fri Oct 27 09:33:36 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Fri Oct 27 09:34:01 2006 Subject: [Histonet] Antibody dilutions and temperature In-Reply-To: Message-ID: Hello all, I have noticed there are different diluents used for various antibodies; I have seen PBS, PBS plus albumin, and PBS/albumin/Tween. What is the difference between using one or the other of these? Also, how does incubation temperature play a role in the choice? Thanks for your answer, Tim :). Actually I would like to hear anyone's opinion since just about everyone here is an IHC or IF expert. Enjoy the weekend! Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From MVaughan4 <@t> ucok.edu Fri Oct 27 09:39:44 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Fri Oct 27 09:40:22 2006 Subject: [Histonet] Melanin problem In-Reply-To: Message-ID: I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From SulkoskyE <@t> monhealthsys.org Fri Oct 27 09:54:41 2006 From: SulkoskyE <@t> monhealthsys.org (Eric Sulkosky) Date: Fri Oct 27 09:53:47 2006 Subject: [Histonet] Steamer Message-ID: <57A8B4592C634A4CAD3A6CB1A0F8103B34DED9@mhsmail2.mhs.org> Hi All My laboratory has been using the rice steamer for years and has not had any problems. I would like to ask, have you been preheating your solutions before trying to retrieve? You mentioned that you were having problems with TTF and Ecad. Are these Dako antibodies? We have found the best results with retrieving, when you preheat your solutions for twenty minutes. Also what pH of solutions are you using? Our procedure for retrieving is as follows: 1. Decide which pH needs to be used. In our laboratory we use High pH 9.9 and Regular pH 6.0 2. For the antibodies you mentioned Ecad and TTF we use the High pH. We have much better results. 3. Set up your steamer, pour your Target Retrieval and preheat in steamer for twenty minutes. 4. For High pH put your slides in the preheated solution and set your timer for 40minutes. Remove the slides from the steamer and leave in your Target Retrieval. Set another clock for 20minutes and let the solution come to room temperature. After it is room temperature, transfer the slides into buffer and let them sit for 5 minutes. This helps from your tissue drying out when staining. 5. For Regular pH put your slides in the preheated solution and set your timer for 20minutes. Remove the slides from the steamer and leave in your Target Retrieval. Set another clock for 20minutes and let the solution come to room temperature. After it is room temperature, transfer the slides into buffer and let them sit for 5 minutes. This helps from your tissue drying out when staining. 6. Then proceed with your staining. Important note: It is very important to let your slides sit in the retrieval and come to room temperature before transferring them into buffer or water. The cooling down is built into the retrieval process, so I would advise not to directly transfer into the buffer right out of the hot steamer. I hope this helps. Eric sulkoskye@monhealthsys.org From liz <@t> premierlab.com Fri Oct 27 10:12:12 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 27 10:08:18 2006 Subject: [Histonet] Melanin problem In-Reply-To: Message-ID: <002101c6f9da$477f9c50$0300a8c0@domain.Premier> Mel When I'm dealing with endogenous brown pigment I switch to a AP detection system and use Fast Red or another red chromagen or if you want to stay with a HRP system then use AEC. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MVaughan4@ucok.edu Sent: Friday, October 27, 2006 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin problem I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1841 (20061027) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From Kari.Zajic <@t> HCAhealthcare.com Fri Oct 27 10:09:49 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Fri Oct 27 10:10:07 2006 Subject: [Histonet] 88331 and 88332 In-Reply-To: Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC7A8@ORLEV03.hca.corpad.net> In my opinion (and experience) you can bill an 88331 x2 because you have two separate specimens in two separate containers. Every additional frozen done on ONE specimen gets charged an 88332, for example if you had done margins on your first (A) specimen x3 frozen blocks, you can charge 88331 x1 and 88332 x2. I am not a professional biller, but this is my understanding. I checked my American Medical Association Surgical Pathology billing guidelines and they (AMA) define pathology specimens as below: "A specimen is defined as tissue or tissues that is (are) submitted for individual and separate attention, requiring individual examination and pathologic diagnosis. Two or more such specimens from the same patient (eg, separately identified endoscopic biopsies, skin lesions, etc.) are each appropriately assigned an individual code reflective of its proper level of service." hope this helps!!! :) Kari Marie Zajic HTL,MLT This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Demarinis, Carolyn Sent: Friday, October 27, 2006 10:14 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] 88331 and 88332 There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 27 10:10:26 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 27 10:10:39 2006 Subject: [Histonet] Antibody dilutions and temperature In-Reply-To: Message-ID: <20061027151026.57585.qmail@web61218.mail.yahoo.com> Mel: 1- Abs + PBS: this is the neutral buffered environment the Ab should be diluted; 2- Abs + PBS + albumen: this is the same environment but the addition of albumen will preserve the Ab better (for a longer time); 3- Abs + PBS + albumen + Tween: the Tween, as a surfactant, will allow the Ab to "spread" better over the section and produce a better staining. You should dilute your Abs in PBS with aldumen (1%) + Tween 20 (0.05%) to obtain better results. Some instruments increase the incubation temperature to obtain quicker results, or the incubation can be done overnight at lower temperatures to allow for weaker dilutions and prevent evaporation. Temperature affects incubation spead according with Van't Hoff's laws (direct effect). When you have many experts in the same field with different opinions, either just 1 is right, or several have a "fraction of the truth" or all are wrong; make your pick! Ren? J. MVaughan4@ucok.edu wrote: Hello all, I have noticed there are different diluents used for various antibodies; I have seen PBS, PBS plus albumin, and PBS/albumin/Tween. What is the difference between using one or the other of these? Also, how does incubation temperature play a role in the choice? Thanks for your answer, Tim :). Actually I would like to hear anyone's opinion since just about everyone here is an IHC or IF expert. Enjoy the weekend! Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Fri Oct 27 10:14:53 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 27 10:21:51 2006 Subject: [Histonet] Melanin problem In-Reply-To: Message-ID: <20061027151453.86842.qmail@web61213.mail.yahoo.com> I used to bleach without affecting antigenicity. Ren? J. MVaughan4@ucok.edu wrote: I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. From gcallis <@t> montana.edu Fri Oct 27 12:46:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Oct 27 12:46:17 2006 Subject: [Histonet] Frozen tissue storage in "Cryosettes" Message-ID: <6.0.0.22.1.20061027114414.01b08a38@gemini.msu.montana.edu> Cruising IHC world, came across a cassette for frozen tissue or frozen block(?) storage, a minicontainer called a Cryosette. It looks promising as a little storage unit for those shopping around or asking for such an item. If anyone has tried them, comments would be welcome. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Margaret.Perry <@t> sdstate.edu Fri Oct 27 12:59:54 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Oct 27 13:00:07 2006 Subject: [Histonet] melanin pigment Message-ID: Mel wrote: I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel We use Vectors Nova Red and it works well with melanin and also hemosiderin. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From Gayle.Haider <@t> dako.com Fri Oct 27 12:57:17 2006 From: Gayle.Haider <@t> dako.com (Gayle Haider) Date: Fri Oct 27 13:03:25 2006 Subject: [Histonet] RE: Melanin problem Message-ID: <8B07D141BCDE434285DC12B3290E3FB384EC34@exbackca.caus.dako.net> Message: 6 Date: Fri, 27 Oct 2006 09:39:44 -0500 Hi Mel, You might want to try an azure B counterstain if you are staining the target antigen with DAB. Azure blue will stain mealnin a blue-green, leaving your target antigens brown from the DAB. Gayle Haider, CLS, MT(ASCP) Technical Support Group Dako North America, Inc. 6392 Via Real Carpinteria, CA 93013 Phone: 805/566-6655 ext. 5323 From: MVaughan4@ucok.edu Subject: [Histonet] Melanin problem To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm Gayle Haider, CLS, MT(ASCP) Technical Support Group Dako North America, Inc. 6392 Via Real Carpinteria, CA 93013 Phone: 805/566-6655 ext. 5323 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, October 27, 2006 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 35, Issue 53 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Steamed About Steamer (Marshall Terry Dr, Consultant Histopathologist) 2. Re: talking of unsolicited emails....... (Geoff McAuliffe) 3. Fwd: In Argentina, Pathologists Don???t Operate Clinical Laboratories (Rene J Buesa) 4. 88331 and 88332 (Demarinis, Carolyn) 5. Antibody dilutions and temperature (MVaughan4@ucok.edu) 6. Melanin problem (MVaughan4@ucok.edu) 7. Steamer (Eric Sulkosky) 8. RE: Melanin problem (Liz Chlipala) 9. RE: 88331 and 88332 (Zajic Kari) 10. Re: Antibody dilutions and temperature (Rene J Buesa) 11. Re: Melanin problem (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Oct 2006 14:23:33 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Steamed About Steamer To: Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C61@LIL.xRothGen.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" By one of those odd coincidences, the next e-mail I received after this one was from my wife who is house sitting whilst her son and family are away, because they have the builders in. It included inter alia: "The rain stopped at midday and it is a lovely afternoon. The roofers are steaming nicely!" Shall I ask her to find out the temperature of boiling water on the roof, and how long they took to get to "steaming"? Have a good weekend everyone. PS Wonder if there are any rice cooker firms listening - I feel a pit>hole coming on. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Fred Underwood [mailto:funderwood@mcohio.org] Sent: 27 October 2006 13:22 To: amosbrooks@gmail.com; histonet@lists.utsouthwestern.edu; Marshall Terry Dr, Consultant Histopathologist Subject: RE: [Histonet] Steamed About Steamer I'm happy to see that this has become a "Nan" issue. >>> "Marshall Terry Dr,Consultant Histopathologist" 10/27/2006 5:40 AM >>> I am intrigued by this discussion. I have been using rice steamers for 42 years. I use them to cook rice, oddly enough:-) They have been used with great frequency - I love my Indian food. Never ever, have I had one that decreased in performance. The only problem has been that the inner pan gets gradually pitted by the salt until it holes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: 27 October 2006 01:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamed About Steamer Hi, I have only used 2 brands of steamer so far but there's no reason another can't work as well. Certainly steamers can wear out as one user has pointed out. I have seen this, the heating element stops getting the temperature high enough. As this is a new steamer that shouldn't be the problem. It's possible that the steaming rack is too far from the element leaving the steam enough time to cooldown before it reaches the slides. That is pretty much a design flaw if that is the case. If so there's nothing else for it but to replace it. Try to get something compact that allows enough space for whatever container you are using to hold the slide rack. It is possible that the vents are allowing the steam out too quickly. That could prevent the steam from heating the buffer up enough. If so you could cover some of the holes (Try duct tape at first, you can figure out something more permanent if it works. To make sure the buffer gets up to the right temperature, I have holes drilled in the top of my steamers just big enough to fit a thermometer. This way I know the buffer is hot enough to put the slides in and when the buffer gets back up to the right temperature after the slides are added. This is great because you can directly monitor the temperature of the solution in real time thruought the retrieval process. (Try that in a microwave or pressure cooker) In response to the gentleman that was having trouble with E-Cadherin, we have found an extra 10 min. produces a stronger antibody reaction. This works for ER & PR too. I am sure we'll find more if we actually get time to test more antibodies. Good Luck Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 27 Oct 2006 09:39:15 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] talking of unsolicited emails....... To: "Andrea T. Hooper" Cc: Histonet Message-ID: <45420C03.9010607@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 I got it too and I also avoid dealing with spammers. Geoff Andrea T. Hooper wrote: > Indeed I did and it irritated me. I make a conscious effort not to > purchase from companies who solicit business by stealing information > off of a listserv and spamming email accounts .... so quite honestly > it's their loss. > > >> Anyone else get the email from Patrick Levasseur, Econo-Lab >> [info@econo-lab.com]? They have clearly harvested emails from >> histonet as I got the mail to my home and work emails, both of which >> are subscribed to histonet. >> >> I am more than happy for vendors to be on the list as they often have >> solutions to things and offer help but I do think harvesting emails >> and putting them on a mailing list for their company is out of order. >> Apparently >> I have to email to be taken off their mailing list too! >> >> Alan Bishop >> Charge scientist >> Histology >> Medlab Central >> Palmerston North >> New Zealand >> >> Tel: 06 952 3135 >> Fax: 06 952 3199 >> > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 3 Date: Fri, 27 Oct 2006 06:49:28 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Fwd: In Argentina, Pathologists Don???t Operate Clinical Laboratories To: histonet@lists.utsouthwestern.edu Message-ID: <20061027134928.79568.qmail@web61215.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Colleagues: I just received this very interesting report that I want to share will all. Ren? J. DARK DAILY wrote: From: "DARK DAILY" To: rjbuesa@yahoo.com Subject: In Argentina, Pathologists Don???t Operate Clinical Laboratories Date: Fri, 27 Oct 2006 13:30:30 +0000 If you are having trouble viewing this email or any of the pages that follow, please open a new browser window and visit: http://www.darkdaily.com/daily/daily_email10.27.06.htm Add the Dark Daily to your address book and never miss an email! Simply click and save the address, Dark_Daily@mail.vresp.com Like our emails? Forward this Email to a friend! Home | Archive | About | Join-- In Argentina, Pathologists Don't Operate Clinical Laboratories And Patients Deliver Their Own Lab Test Reports to Their Doctors DATELINE?BUENOS AIRES, ARGENTINA: Believe it or not, Argentina is a country where pathologists are not associated with clinical laboratory testing! I am Argentina this week to speak to a group of laboratory owners, speak to health insurance executives, and visit clinical laboratories. My hosts on this trip are Sysmex Corporation and Roche Diagnostics Argentina. The most startling thing that I learned is that pathologists do not own clinical laboratories in Argentina, nor do they manage clinical laboratories in hospitals. That responsibility is handled by clinical biochemists. Further, interaction between anatomic pathologists and clinical laboratories is relatively limited. This is a much different situation than the common clinical models and business arrangements used in the United States, Canada, and similar nations. Another interesting difference is that doctors give their patients the laboratory test requisition. Patients then show up at the laboratory of their choice, where specimens are collected. When the results are ready, the laboratory contacts the patient, who comes by the laboratory or patient service center, picks up a paper copy of his or her test report, and then personally delivers the lab test report to his or her physician. This arrangement is counterintuitive to the American system. On the other hand, it does provide a powerful example that patients can reliably play a role this aspect of their healthcare. Competition is intense among private laboratories in Buenos Aires, Argentina. There are private health insurance companies. Labs that bid for provider status in these contracts tend to keep the prices for lab testing at unprofitably low levels. Another factor in the financial struggles of the entire health system in Argentina was the devaluation of the Argentinian Peso in 2001. The purchasing power of the Peso fell by 66% overnight! This has made the purchase of laboratory testing instruments, reagents, and other laboratory consumables prohibitively expensive for labs in Argentina. The effects of this devaluation are still dragging down the finances of this countries' lab industry. During this week, I gained many useful insights about laboratory management, different business models for laboratory testing, and the opportunities for laboratories in the United States to benefit from some of the experience provided by the laboratory testing marketplace in Argentina. These are examples of how innovations by laboratories in one country can be adopted by labs in other countries. I hope to share some of the more powerful innovations with you in coming weeks. Reporting from Buenos Aires, your faithful Editor, Robert L. Michel (E-mail Robert at rmichel@darkdaily.com or schristensen@darkdaily.com.) --------------------------------- Related Products: Executive War College 2007 taking place in Miami, Florida May 10-11, 2007. Topics Archive of DARK Daily The Dark Report The Executive War College Consumer-Directed Health Plans White Paper Dark Daily Explained About Sylvia Christensen About Robert Michel --------------------------------- You are receiving this Email Alert because you are subscribed to DARK DAILY. If you wish to Unsubscribe --------------------------------- DARK DAILY 21806 Briarcliff Dr Spicewood, Texas 78669 Read the VerticalResponse marketing policy. --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. ------------------------------ Message: 4 Date: Fri, 27 Oct 2006 10:14:06 -0400 From: "Demarinis, Carolyn" Subject: [Histonet] 88331 and 88332 To: Message-ID: Content-Type: text/plain; charset="US-ASCII" There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. ------------------------------ Message: 5 Date: Fri, 27 Oct 2006 09:33:36 -0500 From: MVaughan4@ucok.edu Subject: [Histonet] Antibody dilutions and temperature To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello all, I have noticed there are different diluents used for various antibodies; I have seen PBS, PBS plus albumin, and PBS/albumin/Tween. What is the difference between using one or the other of these? Also, how does incubation temperature play a role in the choice? Thanks for your answer, Tim :). Actually I would like to hear anyone's opinion since just about everyone here is an IHC or IF expert. Enjoy the weekend! Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ------------------------------ Message: 6 Date: Fri, 27 Oct 2006 09:39:44 -0500 From: MVaughan4@ucok.edu Subject: [Histonet] Melanin problem To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ------------------------------ Message: 7 Date: Fri, 27 Oct 2006 10:54:41 -0400 From: "Eric Sulkosky" Subject: [Histonet] Steamer To: Message-ID: <57A8B4592C634A4CAD3A6CB1A0F8103B34DED9@mhsmail2.mhs.org> Content-Type: text/plain; charset="us-ascii" Hi All My laboratory has been using the rice steamer for years and has not had any problems. I would like to ask, have you been preheating your solutions before trying to retrieve? You mentioned that you were having problems with TTF and Ecad. Are these Dako antibodies? We have found the best results with retrieving, when you preheat your solutions for twenty minutes. Also what pH of solutions are you using? Our procedure for retrieving is as follows: 1. Decide which pH needs to be used. In our laboratory we use High pH 9.9 and Regular pH 6.0 2. For the antibodies you mentioned Ecad and TTF we use the High pH. We have much better results. 3. Set up your steamer, pour your Target Retrieval and preheat in steamer for twenty minutes. 4. For High pH put your slides in the preheated solution and set your timer for 40minutes. Remove the slides from the steamer and leave in your Target Retrieval. Set another clock for 20minutes and let the solution come to room temperature. After it is room temperature, transfer the slides into buffer and let them sit for 5 minutes. This helps from your tissue drying out when staining. 5. For Regular pH put your slides in the preheated solution and set your timer for 20minutes. Remove the slides from the steamer and leave in your Target Retrieval. Set another clock for 20minutes and let the solution come to room temperature. After it is room temperature, transfer the slides into buffer and let them sit for 5 minutes. This helps from your tissue drying out when staining. 6. Then proceed with your staining. Important note: It is very important to let your slides sit in the retrieval and come to room temperature before transferring them into buffer or water. The cooling down is built into the retrieval process, so I would advise not to directly transfer into the buffer right out of the hot steamer. I hope this helps. Eric sulkoskye@monhealthsys.org ------------------------------ Message: 8 Date: Fri, 27 Oct 2006 09:12:12 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Melanin problem To: , Message-ID: <002101c6f9da$477f9c50$0300a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" Mel When I'm dealing with endogenous brown pigment I switch to a AP detection system and use Fast Red or another red chromagen or if you want to stay with a HRP system then use AEC. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MVaughan4@ucok.edu Sent: Friday, October 27, 2006 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin problem I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1841 (20061027) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com ------------------------------ Message: 9 Date: Fri, 27 Oct 2006 11:09:49 -0400 From: "Zajic Kari" Subject: RE: [Histonet] 88331 and 88332 To: "Demarinis, Carolyn" , Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC7A8@ORLEV03.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" In my opinion (and experience) you can bill an 88331 x2 because you have two separate specimens in two separate containers. Every additional frozen done on ONE specimen gets charged an 88332, for example if you had done margins on your first (A) specimen x3 frozen blocks, you can charge 88331 x1 and 88332 x2. I am not a professional biller, but this is my understanding. I checked my American Medical Association Surgical Pathology billing guidelines and they (AMA) define pathology specimens as below: "A specimen is defined as tissue or tissues that is (are) submitted for individual and separate attention, requiring individual examination and pathologic diagnosis. Two or more such specimens from the same patient (eg, separately identified endoscopic biopsies, skin lesions, etc.) are each appropriately assigned an individual code reflective of its proper level of service." hope this helps!!! :) Kari Marie Zajic HTL,MLT This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Demarinis, Carolyn Sent: Friday, October 27, 2006 10:14 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] 88331 and 88332 There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 27 Oct 2006 08:10:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Antibody dilutions and temperature To: MVaughan4@ucok.edu, histonet@lists.utsouthwestern.edu Message-ID: <20061027151026.57585.qmail@web61218.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Mel: 1- Abs + PBS: this is the neutral buffered environment the Ab should be diluted; 2- Abs + PBS + albumen: this is the same environment but the addition of albumen will preserve the Ab better (for a longer time); 3- Abs + PBS + albumen + Tween: the Tween, as a surfactant, will allow the Ab to "spread" better over the section and produce a better staining. You should dilute your Abs in PBS with aldumen (1%) + Tween 20 (0.05%) to obtain better results. Some instruments increase the incubation temperature to obtain quicker results, or the incubation can be done overnight at lower temperatures to allow for weaker dilutions and prevent evaporation. Temperature affects incubation spead according with Van't Hoff's laws (direct effect). When you have many experts in the same field with different opinions, either just 1 is right, or several have a "fraction of the truth" or all are wrong; make your pick! Ren? J. MVaughan4@ucok.edu wrote: Hello all, I have noticed there are different diluents used for various antibodies; I have seen PBS, PBS plus albumin, and PBS/albumin/Tween. What is the difference between using one or the other of these? Also, how does incubation temperature play a role in the choice? Thanks for your answer, Tim :). Actually I would like to hear anyone's opinion since just about everyone here is an IHC or IF expert. Enjoy the weekend! Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. ------------------------------ Message: 11 Date: Fri, 27 Oct 2006 08:14:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Melanin problem To: MVaughan4@ucok.edu, histonet@lists.utsouthwestern.edu Message-ID: <20061027151453.86842.qmail@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to bleach without affecting antigenicity. Ren? J. MVaughan4@ucok.edu wrote: I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 53 **************************************** From JosefaNava <@t> texashealth.org Fri Oct 27 13:50:17 2006 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Fri Oct 27 13:50:26 2006 Subject: [Histonet] exchange/ trade controls for HSV Message-ID: <2C515C1049EAF5459EFD8C9B929078A401DA13CD@phdex03.txhealth.org> Hello Everyone, I am looking for HSV controls. I can exchange it with either CMV, calcitonin, AFB or whatever you need that I might have. Please let me know. Thank you. Josie Nava Presbyterian Hospital of Dallas The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From mcauliff <@t> umdnj.edu Fri Oct 27 13:58:05 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Oct 27 14:03:58 2006 Subject: [Histonet] Mouse Tissue Problem In-Reply-To: <11399107.1161808797603.JavaMail.ocsadmin@jcs-mid-prod.jax.org> References: <11399107.1161808797603.JavaMail.ocsadmin@jcs-mid-prod.jax.org> Message-ID: <454256BD.3070200@umdnj.edu> Hi Judi All of the possible sources you mentioned could be the cause of your problems. I suggest re-embedding with fresh solutions for all steps. That will circumvent all of the problems exception fixation, which cannont be solved with this batch of tissue. In my experience, properly fixed and processed tissue should not have to be soaked for more than a minute or two to get good sections. However, mouse tissues processed on a processer with a "human tissue schedule" are often overly processed and wind up dry and brittle. I don't know what Telly's fixative is, perhaps a formalin-alcohol-acetic mix? Bouin's fixed material should cut beautifully. Geoff judi.ford@jax.org wrote: >Hi Everyone, > >Our lab has encountered a problem with a batch of mouse tissue processed recently. We receive tissue from various research labs and it is usually either fixed in Telly's or Bouin's for 24 hrs before processing. Nothing unusual was noticed until facing this batch of blocks. Some blocks faced fine while others, like muscle and tumors, shredded and the tissue was soft and spongy (light colored in the middle). The blocks I have faced fine and were hydrated on an ice tray prior to sectioning. In the past I've hydrated bouin's fixed blocks for a couple of days and never had a problem with cutting. These blocks were hydrated in the frig overnight, which is my usual pattern, and then cut the next day. I'm having a horrible time cutting the tissue. Intestines (not impacted with feces) are not cutting well (very compressed with a new knife) and the tissue breaks away from the paraffin both in the block and on the waterbath. The surface of the faced tissue appears wavy after hydration. My water bath temperature is 40 degrees centigrade. > >We've been discussing the following as possible causes: inadequate fixation, fixative mixed wrong, inadequate levels of solutions in the processor (although tissue on the top layer came out fine), tissue not cleared properly, etc. > >I hoping someone may have some ideas on how to narrow down our troubleshooting with this problem. > >Thanks in advance. > >Judi Ford >HIstotechnologist >The Jackson Laboratory >Bar Harbor, Me 04609 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Fri Oct 27 14:47:39 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Oct 27 14:47:46 2006 Subject: [Histonet] Frozen tissue storage in "Cryosettes" In-Reply-To: References: <6.0.0.22.1.20061027114414.01b08a38@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20061027134224.01b44040@gemini.msu.montana.edu> Christie, On IHC World, webpage with Protocol Database, a little advertisement for frozen tissue storage. The cryosette is a round lid, with square back that has label on end. Clicked on box, and a photograph came up, plus purchasing information. Can you fit an OCT embedded block into the cassette? The photograph was not very clear on this, but they do send samples. At 01:20 PM 10/27/2006, you wrote: >Hi Gayle >Where in IHC world did you see a reference to cryosettes. We use something >called a cryosette and I was wondering if it is the same thing? If it is, >I will be happy to tell you of our experiences with them. >Christie Gowan > > >>From: Gayle Callis >>To: Histonet@lists.utsouthwestern.edu >>Subject: [Histonet] Frozen tissue storage in "Cryosettes" >>Date: Fri, 27 Oct 2006 11:46:05 -0600 >> >>Cruising IHC world, came across a cassette for frozen tissue or frozen >>block(?) storage, a minicontainer called a Cryosette. It looks promising >>as a little storage unit for those shopping around or asking for such an item. >> >>If anyone has tried them, comments would be welcome. >> >>Gayle Callis >>MT,HT,HTL(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University - Bozeman >>PO Box 173610 >>Bozeman MT 59717-3610 >>406 994-6367 >>406 994-4303 (FAX) >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lrichey <@t> u.washington.edu Fri Oct 27 15:00:03 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Fri Oct 27 15:00:13 2006 Subject: [Histonet] melanin pigment In-Reply-To: References: Message-ID: <45426543.7060400@u.washington.edu> We use DAB and do a giemsa counterstain. The melanin pigment turns a dark green with the giemsa. Perry, Margaret wrote: >Mel wrote: > >I don't recall this question, but I would be interested in how you get >around the problem of epidermal pigmentation if you are looking for >protein expression in the epidermis; do you bleach the tissue (and if >so, does that affect antigenicity of the proteins), or can you choose a >detection chromogen that has a wild color, like a Barney purple color? > >Thanks for your help. > >Mel > > > >We use Vectors Nova Red and it works well with melanin and also >hemosiderin. > > > >Margaret Perry HT (ASCP) > >IHC Lab Manager Veterinary Science > >Animal Disease Research and Diagnostic Lab > >South Dakota State University > >Box 2175 North Campus Drive > >Brookings SD 57007 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From arvidsonkristen <@t> yahoo.com Sat Oct 28 08:14:18 2006 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sat Oct 28 08:14:23 2006 Subject: [Histonet] Non-fading pens Message-ID: <20061028131418.97780.qmail@web61311.mail.yahoo.com> We are looking for slide/block pens. Those we've used in the past seem to fade during processing. Any suggestions? --------------------------------- Low, Low, Low Rates! Check out Yahoo! Messenger's cheap PC-to-Phone call rates. From RSRICHMOND <@t> aol.com Sat Oct 28 21:11:08 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Oct 28 21:11:15 2006 Subject: [Histonet] 88331 and 88332 frozen section CPT codes (USA) Message-ID: Carolyn Demarinis asks: There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. If two specimens are sent to the lab for frozen section labeled: A)?? ? left upper eyelid 12 o'clock marker B)? ? ? left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. *********************** Code 88331 for the first FS, and an 88332 for each additional frozen section on the case. And an 88305 for each specimen. Just as the rejection letter stated. Documentation: the CPT coding manual. You do have one, don't you? If your pathologists don't know how to code, they should learn how. You should be checking their coding (most of us make plenty of errors in coding), rather than being the primary coder. Bob Richmond Samurai Pathologist Knoxville TN From hej01 <@t> health.state.ny.us Mon Oct 30 07:53:57 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Oct 30 07:54:21 2006 Subject: [Histonet] + slides Message-ID: Hi Histonetters, Can you tell me what adhesive slide would work best for mosquito specimens. I will be doing IHC. Helen Johnson (hej01@health.state.ny.us) From Rcartun <@t> harthosp.org Mon Oct 30 08:21:31 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 30 08:22:16 2006 Subject: [Histonet] 88331 and 88332 In-Reply-To: References: Message-ID: <4545C41B020000770000297A@hcnwgwds01.hh.chs> One could argue that is all one specimen and, therefore, should be coded as follows: 88331, 88332, and 88305. In my opinion, it doesn't make any sense to allow 88305x2 if you are going to allow 88331 and 88332. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Demarinis, Carolyn" 10/27/06 10:14 AM >>> There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Mon Oct 30 08:55:21 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Oct 30 08:55:54 2006 Subject: [Histonet] Workshop on unusual beasties Message-ID: After having seen several queries for help come across my email via listserve mails, I am totally fascinated with how histology is done on usual (to me) species, like shrimp or other sea creatures, or insects (mosquitos!) or plants etc... I would be most interested in attending a workshop, NSH or otherwise, on how you do histology on these creatures. Any takers? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From alaskagirl1950 <@t> yahoo.com Mon Oct 30 09:07:48 2006 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Mon Oct 30 09:08:04 2006 Subject: [Histonet] Workshop on unusual beasties In-Reply-To: Message-ID: <20061030150748.42020.qmail@web52507.mail.yahoo.com> Teri, This sounds great to me! I have recently left human creatures, for a Vet School and animal research. Things are so,... many legged. I would love to learn all the differences, and how to overcome. Patrica Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Get your email and see which of your friends are online - Right on the New Yahoo.com (http://www.yahoo.com/preview) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 30 09:13:28 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 30 09:12:41 2006 Subject: [Histonet] Workshop on unusual beasties Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC875@wahtntex2.waht.swest.nhs.uk> Anyone know about mouth tumours in cat's? My cat is 16 and has had a mouth lesion resected, what do they get? Seems to be around a tooth. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Prayer is a radical response to the mysteries of life. --Matthew Fox This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From bakevictoria <@t> gmail.com Mon Oct 30 09:41:42 2006 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Oct 30 09:41:54 2006 Subject: [Histonet] Surgical Pathology turn around time survey programs Message-ID: <4f016b690610300741m756086f1mf90f4970c73647bb@mail.gmail.com> Good Morning! I'm looking to see if anyone is currently tracking surgical path turn around times. I can remember using something from either ASCP or CAP that tracked specimens from point of entry (gross room accession) to final sign out back in the early 90's. It was a random selection of specimens and had to include all levels of complexity (ie- SNOMEDs and institutional requirements for special procedures). We did this quarterly and our results were then measured against other institutions that were similar to ours. It was a lot of work to do, but a great tool for improving services. Thanks. Vikki From algranth <@t> u.arizona.edu Mon Oct 30 09:50:48 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Oct 30 09:51:08 2006 Subject: [Histonet] + slides In-Reply-To: Message-ID: <4.3.2.7.2.20061030084729.00cc9578@algranth.inbox.email.arizona.edu> Helen, I've been cutting whiteflies and putting the sections on HistoBond Slides from StatLab. Works fine and the lab is even doing in-situ and the little whiteflies stay on just fine. Now, Would you be able to share some insect processing protocols? How do you get by their hard outer surfaces? We are trying to use DMSO but I'm wondering if there is any other way??? Andi Grantham At 08:53 AM 10/30/2006 -0500, you wrote: >Hi Histonetters, > Can you tell me what adhesive slide would work best for mosquito >specimens. I will be doing IHC. > Helen Johnson >(hej01@health.state.ny.us) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From immrstambo <@t> hotmail.com Mon Oct 30 09:08:02 2006 From: immrstambo <@t> hotmail.com (Chistine Tambasco) Date: Mon Oct 30 10:38:03 2006 Subject: [Histonet] 88331 and 88332 References: Message-ID: Carolyn - I agrre with you. If there are 2 separte skins, not in the same container, it should be 88305 X2. Also, if the frozen section is on 2 separate skins it should be 88331 X2. If they are received in the same container, then that's a different story. Take Care, your friend, Chrssy P.S. I do all the billing for my Pathologist, I only ask him if I dont have enough info from the CPT quide. Christine Tambasco, HT (ASCP) St. Mary's Hospital Amsterdam, New York ----- Original Message ----- From: "Demarinis, Carolyn" To: Sent: Friday, October 27, 2006 9:14 AM Subject: [Histonet] 88331 and 88332 There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Oct 30 10:45:42 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Oct 30 10:45:56 2006 Subject: Help on Re: [Histonet] Workshop on unusual beasties In-Reply-To: <20061030150748.42020.qmail@web52507.mail.yahoo.com> References: <20061030150748.42020.qmail@web52507.mail.yahoo.com> Message-ID: <6.0.0.22.1.20061030093631.01b4f7c8@gemini.msu.montana.edu> For those going from clinical hospital histolabs and into research with animals, there is an Animal Processing Manual available from National Society for Histotechnology. Go to www.nsh.org for particulars on how to order this manual, it is not overly expensive. It contains recommendations and guidelines for processing and fixation for all kinds of beasties, particularly marine animals and many other species. Another good textbook is Humanson's Animal Tissue Techniques, Fifth Edition by Presnell JK and Schreibman MP, ISBN# 0-8018-5401-6. Also, if you are a member of NSH, you are can join the Veterinary Research and Industry (VIR) committee at no extra cost to access a membership list of people who list their expertises. You can then contact them personally for information on animal histotechniques. The NSH Hard Tissue committee is the same situation for those working on implants, bone, stents, plastics. At 08:07 AM 10/30/2006, you wrote: >Teri, >This sounds great to me! >I have recently left human creatures, for a Vet >School and animal research. Things are so,... >many legged. >I would love to learn all the differences, and >how to overcome. >Patrica > >Patricia Adams >----- >Fight back spam! Download the Blue Frog. >http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > > >____________________________________________________________________________________ >Get your email and see which of your friends are online - Right on the New >Yahoo.com >(http://www.yahoo.com/preview) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From HornHV <@t> archildrens.org Mon Oct 30 10:46:11 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Oct 30 10:46:42 2006 Subject: [Histonet] 88331 and 88332 In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6E63@EMAIL.archildrens.org> We bill 88331 for the 1st frozen and 88332 for each additional frozen even if there are multiple specimens. We never bill for two 88331's on the same patient. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chistine Tambasco Sent: Monday, October 30, 2006 9:08 AM To: Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: Re: [Histonet] 88331 and 88332 Carolyn - I agrre with you. If there are 2 separte skins, not in the same container, it should be 88305 X2. Also, if the frozen section is on 2 separate skins it should be 88331 X2. If they are received in the same container, then that's a different story. Take Care, your friend, Chrssy P.S. I do all the billing for my Pathologist, I only ask him if I dont have enough info from the CPT quide. Christine Tambasco, HT (ASCP) St. Mary's Hospital Amsterdam, New York ----- Original Message ----- From: "Demarinis, Carolyn" To: Sent: Friday, October 27, 2006 9:14 AM Subject: [Histonet] 88331 and 88332 There seems to be some confusion on what codes to use when multiple frozens are sent on multiple specimens. I need some clarification, therefore, I am providing an example. If two specimens are sent to the lab for frozen section labeled: A) left upper eyelid 12 o'clock marker B) left upper eyelid Frozen section diagnosis on A) is basal cell carcinoma at margins. Frozen section diagnosis on B) is no evident malignancy. Am I correct in using 88331 x 2 in addition to 88305 x 2? I am getting billing rejections stating that I should be using 88331 x 1, 88332 x 1, 88305 x 2. If so, please provide me with appropriate documentation. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From David.Edmondson <@t> christie-tr.nwest.nhs.uk Mon Oct 30 12:01:28 2006 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Mon Oct 30 12:05:02 2006 Subject: [Histonet] melanin pigment Message-ID: <3C83687E8F6AE04792E361ABE2D385B84181D2@cht-mail2-2k.xchristie.nhs.uk> Last week we used a Mallory bleach to remove the melanin, which worked well enough for Mart-1(Melan A) but not for HMB45. We then use a VIP purple DAB product ( from Vector) on another set of slides without the bleach. Whatever it takes Dave Christie Hosp, Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: 27 October 2006 19:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] melanin pigment Mel wrote: I don't recall this question, but I would be interested in how you get around the problem of epidermal pigmentation if you are looking for protein expression in the epidermis; do you bleach the tissue (and if so, does that affect antigenicity of the proteins), or can you choose a detection chromogen that has a wild color, like a Barney purple color? Thanks for your help. Mel We use Vectors Nova Red and it works well with melanin and also hemosiderin. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From amylee779 <@t> yahoo.com Mon Oct 30 12:07:49 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Oct 30 12:08:00 2006 Subject: [Histonet] EGFR IHC Message-ID: <20061030180749.37088.qmail@web58301.mail.re3.yahoo.com> Hi, I was asked to do EGFR IHC on FFPE mouse tumor tissue. This is new to me. There are many antibodies available on the market. Is Zymed 28-0005 one good for FFPE IHC? Do you mind share your opinon if you did this staining before? Which antibody is the best? Thanks in advance! Amy --------------------------------- Check out the New Yahoo! Mail - Fire up a more powerful email and get things done faster. From MVaughan4 <@t> ucok.edu Mon Oct 30 12:07:48 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Mon Oct 30 12:08:19 2006 Subject: [Histonet] Melanin, fast red, and a counterstain In-Reply-To: Message-ID: Thanks to everyone who answered the questions about melanin pigment. It seems most of you use a red pigmented chromogen to demonstrate IHC staining that will be easy to differentiate from melanin. I should have asked this question at the same time: do the red chromogens work all right with a hematoxylin counterstain, with a methyl green counterstain, or can I do without the counterstain entirely? Thanks again. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From aep10 <@t> cornell.edu Mon Oct 30 12:26:23 2006 From: aep10 <@t> cornell.edu (Anna Elisse Beaudin) Date: Mon Oct 30 12:26:38 2006 Subject: [Histonet] Timms stain in fresh frozen cryosections Message-ID: <1254.132.236.104.213.1162232783.squirrel@webmail.cornell.edu> Hello, I would like to do Timm's stain in fresh frozen cryosections of mouse brain. The only protocol I could find involves incubating the sections O/N in a dessication chamber with highly toxic H2S gas (created by adjusting a 0.1% Na2S solution to pH 7.3) prior to rehydration followed by classic timms staining protocol. I was wondering if anybody had any other protocols and/or the best way to do this without poisoning myself and my lab mates :) I really appreciate any help! Thanks in advance, Anna Beaudin Division of Nutritional Sciences Cornell University From gcallis <@t> montana.edu Mon Oct 30 12:38:22 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Oct 30 12:38:36 2006 Subject: [Histonet] Melanin, fast red, and a counterstain In-Reply-To: References: Message-ID: <6.0.0.22.1.20061030112628.01b33480@gemini.msu.montana.edu> Mel, Red chromogens are excellent for giving a red/blue contrast with hematoxylin, and having the melanin pigment there too, you will have three colors. Our researchers prefer red chromogens with hematoxylin, and we never use methyl green with red, but have used it with enhanced DAB (black color). Most of our researchers are used to seeing hematoxylin stained sections and this counterstain is helpful for additional morphology examination. Be sure to not be heavy handed with the hematoxylin, you want to LIGHTLY counterstain so you don't mask your red immunostained cells. You can always counterstain one slide with hematoxylin and not counterstain an adjacent section just to see the different color contrasts, you may actually prefer NO counterstaining. We never use methyl green with red chromogens, but have used it with brown DAB or an enhanced black DAB. Be careful, methyl green may leach out of the section with a red AEC when you coverslip with aqueous mounting media as AEC will wash out/fade in alcohol or clearing agents. Vector NOVA red is permanent and will not fade after exposure to solvents. So be aware of mounting media compatibility with the chosen chromogen. At 11:07 AM 10/30/2006, you wrote: >Thanks to everyone who answered the questions about melanin pigment. It >seems most of you use a red pigmented chromogen to demonstrate IHC >staining that will be easy to differentiate from melanin. I should have >asked this question at the same time: do the red chromogens work all right >with a hematoxylin counterstain, with a methyl green counterstain, or can >I do without the counterstain entirely? Thanks again. >Mel >Melville B. Vaughan, Ph. D. >Assistant Professor >Department of Biology >University of Central Oklahoma >100 N. University Drive >Edmond, OK 73034 >http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From esther.peters <@t> verizon.net Mon Oct 30 13:12:19 2006 From: esther.peters <@t> verizon.net (Esther Peters) Date: Mon Oct 30 13:14:57 2006 Subject: [Histonet] Workshop on unusual beasties References: Message-ID: <45464E93.4090404@verizon.net> Dear Teri and All, Preparing histoslides of diverse creatures is not that different from what is done for humans! NSH's VIR Committee developed a manual that is available from NSH, providing many protocols and insights: Callis, G., and Sterchi, D., eds. 2002. Animal Processing Manual. National Society for Histotechnology: Veterinary, Industry and Research Committee, Bowie, MD. We have also published methods for invertebrates: Peters, E.C., K.L. Price, and D.J. Borsay Horowitz. 2005. Histological preparation of invertebrates for evaluating contaminant effects. In Techniques in Aquatic Toxicology, Volume 2, ed. G.K. Ostrander, pp. 653-686. Taylor & Francis, Boca Raton, FL. And there are other resources available for other groups of organisms. As with human tissue samples, the main concerns are "what tissue is needed" to guide trimming and processing and "why are you doing this" that guides selection of fixative and staining protocols to be used (histochemical, IHC, or other) to obtain the data you need for the question you want to answer. Not sure there has been an NSH workshop on this recently, although I have attended some in the past. Kathy Price and I hope to teach a workshop on histotechniques for anthozoans (sea anemones, corals) in Hawaii next summer, if it can be scheduled. Esther Peters, Ph.D. George Mason University and Registry of Tumors in Lower Animals Johnson, Teri wrote: > After having seen several queries for help come across my email via > listserve mails, I am totally fascinated with how histology is done on > usual (to me) species, like shrimp or other sea creatures, or insects > (mosquitos!) or plants etc... > > I would be most interested in attending a workshop, NSH or otherwise, on > how you do histology on these creatures. Any takers? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From funderwood <@t> mcohio.org Mon Oct 30 14:25:34 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Mon Oct 30 14:26:07 2006 Subject: [Histonet] Non-fading pens Message-ID: I use the HistoTec Pen from Newcomer Supply. newcomersupply.com or 1-800-383-7799 Fred >>> kristen arvidson 10/28/2006 9:14 AM >>> We are looking for slide/block pens. Those we've used in the past seem to fade during processing. Any suggestions? --------------------------------- Low, Low, Low Rates! Check out Yahoo! Messenger's cheap PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From julie.blake <@t> holburn.com Mon Oct 30 14:31:59 2006 From: julie.blake <@t> holburn.com (Julie Blake) Date: Mon Oct 30 14:32:34 2006 Subject: [Histonet] Histology Job Postings - Ontario Message-ID: THE HOLBURN GROUP OF COMPANIES, an expanding research and development firm located in the Municipality of Clarington, Durham Region, is focused on innovative research and development programmes in the fields of diagnostics and therapeutics. The projects we undertake both independently and in collaboration with academia and private industry range from early-stage drug discovery to product delivery. We are looking for results-driven professionals to join our team! HISTOLOGIST As a Histologist, reporting to a Team Leader, you will undertake various histological procedures. Qualifications * Minimum three years of histology experience preferred. * B.Sc. in Medical Laboratory Sciences and/or MLT, ART or equivalent designation. * Highly motivated, flexible and able to thrive in a fast paced environment. * Strong problem-solving, analytical and planning skills, with demonstrated superior written skills We offer a salary commensurate with skills and experience, a comprehensive benefits package and opportunities for growth and professional development. Please submit a covering letter referencing the position and your curriculum vitae in confidence to hr@holburn.com or by fax to 905-623-6702, attention Human Resources. Thank you in advance for your interest. Only those candidates selected for an interview will be contacted. We are an equal opportunity employer. Application date deadline: November 10, 2006. Human Resources Coordinator Holburn Group of Companies Fax: 905 623 6702 E-mail: hr@holburn.com Web: www.holburn.com From julie.blake <@t> holburn.com Mon Oct 30 14:35:58 2006 From: julie.blake <@t> holburn.com (Julie Blake) Date: Mon Oct 30 14:36:11 2006 Subject: [Histonet] Histology Job Postings - Ontario Message-ID: THE HOLBURN GROUP OF COMPANIES, an expanding research and development firm located in the Municipality of Clarington, Durham Region, is focused on innovative research and development programmes in the fields of diagnostics and therapeutics. The projects we undertake both independently and in collaboration with academia and private industry range from early-stage drug discovery to product delivery. We are looking for results-driven professionals to join our team! Team Leader, Histology Laboratory As TEAM LEADER, Histology Laboratory, reporting to the Scientific Director, you will be responsible for the efficient and effective management of all laboratory operations and laboratory staff. Additional responsibilities include: development, implementation and audit of SOPs, regulatory standards and quality control systems; and training new staff. * Minimum 3-5 years practical histochemical experience required. Immunocytochemical and in situ hybridization techniques experience is preferred. * M.Sc. preferred and/or MLT or ART designation. * Highly motivated, flexible and able to thrive in a fast paced environment. * Strong problem-solving, analytical and planning skills, with demonstrated superior written skills * Demonstrated ability to successfully manage and implement major projects. A working knowledge of immunohistochemistry, molecular biology (in situ hybridization) and automated histotechnology is desirable. Base Salary: $70,000-$80,000 annually, plus comprehensive benefits package and opportunities for growth and professional development. ? Please submit a covering letter referencing the position and your curriculum vitae in confidence to hr@holburn.com or by fax to 905-623-6702, attention Human Resources. ? Thank you in advance for your interest. Only those candidates selected for an interview will be contacted. We are an equal opportunity employer. Application deadline date: November 10, 2006. Human Resources Coordinator Holburn Group of Companies Fax: 905 623 6702 E-mail: hr@holburn.com Web: www.holburn.com From TillRenee <@t> uams.edu Mon Oct 30 14:38:08 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Mon Oct 30 14:38:37 2006 Subject: [Histonet] dissecting tiny tissues Message-ID: <11F927674DEBDC43B960809A7403C5D2025E0707@MAILPED.ad.uams.edu> Does anyone have any experience with dissecting fetal rat tissues? We need to collect the mammary gland of a gestation day 18 rat fetus. I was told it might require using a dissecting microscope. I have never collected tissues from anything smaller than a post natal day 21 rat, so I would appreciate any help you could offer. I believe we could manage it if I knew exactly what to look for or what there is too see on the rat at that age. I know others who take tissues like the liver, but that is not quite the same as locating and taking out the mammary gland. Do you think if we took the skin and fixed it, we would be able to get the gland off later (within a day or two)? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From galalmkh <@t> yahoo.com Mon Oct 30 15:21:17 2006 From: galalmkh <@t> yahoo.com (manal galal) Date: Mon Oct 30 15:21:30 2006 Subject: [Histonet] PAS on frozen muscle Message-ID: <20061030212117.41224.qmail@web50209.mail.yahoo.com> hi histonetters, does anyone have a picture of a normal and abnormal PAS stained frozen muscle biopsy thanks Dr. Galal (pathologist) --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From barry_m <@t> ozemail.com.au Mon Oct 30 16:54:54 2006 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Mon Oct 30 16:55:11 2006 Subject: [Histonet] HER2 CISH Message-ID: <000701c6fc76$6b4e6c80$0201010a@WORKSTATION1> Hi fellow histonetters, There has been much discussion here in Brisbane between a number of histology laboratories, concerning the thickness of breast tissue sections for HER2 CISH. My question is then, what thickness should they be cut at? Regards, Barry Madigan Immunohistochemistry QHPS-Central Royal Brisbane Hospital Australia From laurie.colbert <@t> huntingtonhospital.com Mon Oct 30 17:46:59 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Oct 30 17:47:11 2006 Subject: [Histonet] ACIS Billing Message-ID: <57BE698966D5C54EAE8612E8941D768319980E@EXCHANGE3.huntingtonhospital.com> What do others charge for IHC staining that is subsequently run for Image Analysis? We use the Clarient system. There has been much discussion here regarding the CPT codes 88342, 88358, and 88361. We are using all three codes x6 (except the 88358, which is x1). Can anyone educate me on this issue? Laurie Colbert From dbpiontek <@t> hotmail.com Mon Oct 30 20:19:41 2006 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Mon Oct 30 20:48:08 2006 Subject: [Histonet] Leica Autostainer XL and Leica CV5030 glass coverslipper References: Message-ID: Hi Lisa: We have the Leica with attached coverslipper and absolutely love it. Clean your coverslipping racks once a week by soaking in xylene to remove excess mounting media and store coverslips in desicator to avoid condensation. I have had no problems. Hope this helps, Denise Bland-Piontek, HTL(ASCP)CTBS(AATB) Toxikon Corporation ----- Original Message ----- From: Lisa Brenner To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 26, 2006 8:42 AM Subject: [Histonet] Leica Autostainer XL and Leica CV5030 glass coverslipper Hello all, Do any of you use the Leica autostainer XL? What are your likes/dislikes? How does it compare to Sakura? Do any of you have the Leica glass coverslipper? Likes or dislikes especially if you have it connected to Leica stainer. We also have a Thin prep imager. Do any of you use the Leica coverslipper with the glass coverslips with the imager? Does it read the slides okay and do you have any problems with cornflaking? Any feed back will be greatly appreciated. We need to make up our mind soon and the powers that be are pushing us to hurry up. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hoho.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Tue Oct 31 05:49:36 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Oct 31 05:49:46 2006 Subject: [Histonet] NSH 2007 In-Reply-To: <200610261707.k9QH7WJu014924@pro12.abac.com> Message-ID: Patsy, I just returned from a weekend in Denver and I cannot wait to go back for NSH in 2007. I left on Thursday of the snowstorm and was delayed for about 2 hours in Houston but once we got there the snow was already melting and the rest of the weekend was absolutely GLORIOUS! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy ruegg Sent: Thursday, March 31, 2005 12:07 AM To: histonet@pathology.swmed.edu Subject: [Histonet] NSH 2007 For all ya'll thinking about coming to the NSH S/C in Denver at this time next year don't let our snow storm today scare you off, it is supposed to be in the 60's tomorrow. Dress in layers is what we say around here. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> bidmc.harvard.edu Tue Oct 31 09:16:27 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Tue Oct 31 09:18:47 2006 Subject: [Histonet] marker for brain inflammation Message-ID: <3665A19D-43E0-4583-A345-93EE8AE4C30D@bidmc.harvard.edu> Hello, I'm wondering if someone could suggest a marker or stain that could help visualize inflammation. I am working on a project where I inject a virus in the brain to introduce genes in neurons. However, I want to make sure that the injection itself, or the neuronal infection is not causing a large degree of inflammation. Could someone suggest a good way to visualize this? I imagine some sort of immune marker will do, perhaps mF4/80? thanks, Caroline From Charlene.Henry <@t> STJUDE.ORG Tue Oct 31 09:51:17 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Oct 31 09:51:30 2006 Subject: [Histonet] Serum Fiber in Blood Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1EA1@SJMEMXMB02.stjude.sjcrh.local> Our mouse pathologist has previously ordered the PTAH stain for blood serum fiber in mouse tissues; however we plan to retire this stain because of the mercury used in the stain. This is the last place in the lab that requires mercury. My question is: do any of you know of another special stain or antibody that will detect blood serum fiber in FFPE mouse tissue? Thanks, Charlene From gcallis <@t> montana.edu Tue Oct 31 10:36:26 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Oct 31 10:36:38 2006 Subject: Fibrin stain Re: [Histonet] Serum Fiber in Blood In-Reply-To: <5CB39BCA5724F349BCB748675C6CA1A2099A1EA1@SJMEMXMB02.stjude .sjcrh.local> References: <5CB39BCA5724F349BCB748675C6CA1A2099A1EA1@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <6.0.0.22.1.20061031093334.01b0cd10@gemini.msu.montana.edu> Martius Yellow also known as MSB stain for fibrin works on FFPE. I will attach the method to you privately. You may also find this on a staining website. Peggy Wenk kindly sent this method to me so a thank you goes out to her. At 08:51 AM 10/31/2006, you wrote: >Our mouse pathologist has previously ordered the PTAH stain for blood >serum fiber in mouse tissues; however we plan to retire this stain >because of the mercury used in the stain. This is the last place in the >lab that requires mercury. My question is: do any of you know of another >special stain or antibody that will detect blood serum fiber in FFPE >mouse tissue? >Thanks, >Charlene > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From histology.bc <@t> shaw.ca Tue Oct 31 10:38:59 2006 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Tue Oct 31 10:40:35 2006 Subject: [Histonet] Serum Fiber in Blood In-Reply-To: <5CB39BCA5724F349BCB748675C6CA1A2099A1EA1@SJMEMXMB02.stjude.sjcrh.local> References: <5CB39BCA5724F349BCB748675C6CA1A2099A1EA1@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <45477C23.3060103@shaw.ca> Henry, Charlene wrote: >Our mouse pathologist has previously ordered the PTAH stain for blood >serum fiber in mouse tissues; however we plan to retire this stain >because of the mercury used in the stain. This is the last place in the >lab that requires mercury. My question is: do any of you know of another >special stain or antibody that will detect blood serum fiber in FFPE >mouse tissue? >Thanks, >Charlene > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > By "blood serum fiber" I assume you are talking about fibrin, the network of coagulation factors involved in clot formation and a variety of inflammatory conditions. PTAH is one of the classical methods for demonstrating fibrin. However, a much "prettier" method is the MSB stain developed by Slidders and Lendrum. MSB is a modified trichrome method that uses martius yellow, brilliant crystal scarlet and methyl blue. Fibrin stains a distinct bright red, red cells are yellow, collagen is blue, with blue grey nuclei. It is one of the most aesthetically pleasing staining methods in use. The original method recommended fixation in a mercury-contaning fixative, as did many methods from years gone by. However, equally good results can be obtained by treating formalin-fixed tissues with Boiun's fixative for about an hour before staining. Just take the sections down to water as usual, treat with Bouin's fixative at 56-60 degrees, wash well in water to remove the picric acid coloration, and carry on with the staining method. There is a good method in StainsFile (courtesy of Bryan Llewellyn). See link below http://stainsfile.info/StainsFile/stain/fibrin/MSB-long.htm Paul Bradbury, FIMLS, ART, Kamloops, BC Canada From mcauliff <@t> umdnj.edu Tue Oct 31 11:00:26 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 31 11:01:13 2006 Subject: [Histonet] marker for brain inflammation In-Reply-To: <3665A19D-43E0-4583-A345-93EE8AE4C30D@bidmc.harvard.edu> References: <3665A19D-43E0-4583-A345-93EE8AE4C30D@bidmc.harvard.edu> Message-ID: <4547812A.2020207@umdnj.edu> Hi Caroline: Hmmm. Sticking a needle into the brain will cause some disruption, bleeding and a normal response to the injury, ie. inflamation. Native microglia will be activated and macrophages and other WBC will enter the wound site via ruptured blood vessels. Both native microglia and arriving macrophages will be positive for F4/80. There will also be an astrocyte reaction, almost certainly visible with GFAP. I suggest a second injection into the same region on the opposite side of the brain. Use the vehicle without the virus as a control to see how much inflamation you get on one side versus the other. You do need a control and this seems like a logical one to me. A lot has been written on injecting various tracers into the brain and I suspect someone has dealt with this issue, but in the 1970's. Get a copy of "Neuroanatomical tract tracing methods" by Heimer and Robards out of the library or try J. Neurosci. Methods 103:1, 2000 and see what has been done. Geoff Caroline Bass wrote: > Hello, > > I'm wondering if someone could suggest a marker or stain that could > help visualize inflammation. I am working on a project where I > inject a virus in the brain to introduce genes in neurons. However, > I want to make sure that the injection itself, or the neuronal > infection is not causing a large degree of inflammation. Could > someone suggest a good way to visualize this? I imagine some sort of > immune marker will do, perhaps mF4/80? > > thanks, > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Eric <@t> ategra.com Tue Oct 31 11:24:01 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Tue Oct 31 11:09:06 2006 Subject: [Histonet] 9 New Openings for Temporary and Permanent Histology Technicians - 15% Pay Raise Guaranteed ! Message-ID: Hi - Fellow-Histonetters - Are you looking for a change ? Below is the updated list of both temp and perm Histology jobs, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Most Histotech jobs are permanent full-time unless indicated otherwise Also, if your not interested in any of these great opportunities and prefer a different location, please call me and let me know where and I can find it for you. --> My Hottest Temporary Histology (Grossing) Assignment Is In Pennsylvania, Great Location, Top Dollar, Call Today- Needs To Be Filled By October 25th 2006 --> My Hottest Permanent Histology Is In Eastern, Massachusetts Seeking Histotechs of all experience levels, Great Pay, Location And Benefits- Call Today - 15% Pay Raise Guaranteed! BrandNew Permanent Histology Jobs: --------------------------------------------- 1. Southwest Florida- Histotech Supervisor- perm - BrandNew (Need Florida License) 2. North Texas- Bench Histotech- Perm BrandNew 3. California (Bay Area) - Bench Histotech- perm -BrandNew 4. Texas (Dallas Area)- Bench Histotech- perm- BrandNew 5. Tennessee (Nashville Suburb) - Bench Histotech- perm- Hard Tissue (Bones) -BrandNew 6. Virginia (Northern) - Bench Histotech - perm- BrandNew 7. Southwest Texas- Bench Histotech- perm - BrandNew 8. Eastern Mass - Bench Histotech I- perm- BrandNew 9. Eastern Mass- Bench Histotech II- perm- BrandNew 10. Washington, D.C. -Bench - Diener- perm- BrandNew (AA Mortuary Science, Diener, 2-5 years Autospy experience.) Current Permanent Histology Jobs: ----------------------------------------- 11. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 12. Northern New Jersey - Histotech - perm 13. Eastern Mass - Histotech - one Senior Histotech, One not so Senior Histotech 14. Eastern Mass - Histotech - Histotech -perm 15. Massachusetts (North of Boston) - perm - Bench Histotech 16. Central Florida -perm- Histotech (Need Florida License) 17. Southeast Florida - Treasure Coast - perm - Histotech (Need Florida License) 18. Southeast Florida - perm - Histotech (Need Florida License) 19. South Florida - perm - Bench Histotech (Need Florida License) 20. Florida, West Coast - both temp & perm openings- Bench Histotech 21. New Hampshire perm openings - Bench Histotech 22. New York ( Syracuse area) - Bench Histotech- perm 23. Central Florida - Bench Histotech- perm -----------------------------------end perm jobs ---------------------------------------------------- Temporary Assignments ------------------------------- 1. Massachusetts (Eastern) - Histo Tech minimum of 13 weeks (2 people) 2. Pennsylvania - Histotech (Grossing)- 6 months -HOT-HOT-HOT -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From freidac <@t> sbcglobal.net Tue Oct 31 11:12:48 2006 From: freidac <@t> sbcglobal.net (Freida Carson) Date: Tue Oct 31 11:13:03 2006 Subject: [Histonet] My book - 1st edition Message-ID: <20061031171248.82762.qmail@web82507.mail.mud.yahoo.com> Does anyone have a copy of my book - Carson: Histotechnology, A Self-Instructional Text, 1st edition, in good condition that they would like to sell? Unfortunately, at our HQIP committee meeting, a borrowed copy of the book belonging to a CAP staff member was thrown away by the cleaning staff. We want the 1st edition only. Please contact me if you have a copy. Thanks, Freida Carson From gliuygao <@t> hotmail.com Tue Oct 31 11:24:11 2006 From: gliuygao <@t> hotmail.com (yan gao) Date: Tue Oct 31 11:24:24 2006 Subject: [Histonet] Please unsubscribe me In-Reply-To: <20061031171248.82762.qmail@web82507.mail.mud.yahoo.com> Message-ID: Please unsubscribe me. Yan Gao _________________________________________________________________ [1]Get FREE company branded e-mail accounts and business Web site from Microsoft Office Live References 1. http://g.msn.com/8HMAENUS/2734??PS=47575 From funderwood <@t> mcohio.org Tue Oct 31 11:38:14 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Tue Oct 31 11:38:43 2006 Subject: [Histonet] steamed about steamer-resolution Message-ID: Hi All, I returned the Oster rice steamer I initially purchased and replaced it with a Black and Decker HS900. Well whadda ya know. I was able to get the retrieval solution up to 99 degrees with no problem. I think the issue is this. The Oster has a plastic sleeve that slips over the heating element. The purpose of this is to decrease the volume of water in direct contact with the heating element, thus speeding the production of steam. However, the amount of steam produced is reduced as well. The sleeve is removable, though the directions explicitly state not to use without the sleeve in place. The Black and Decker has a similar sleeve( it is built into the drip tray) but steaming can be performed without the drip tray. By doing it this way, the proper temperature is achieved. Sorry to be so long winded, but I am no longer steamed. Atleast not on this issue. Thanks again for your input, Fred By the way Terry, it also whips up a mean lamb vindaloo :) From alan <@t> westwoodwilshire.com Tue Oct 31 13:19:54 2006 From: alan <@t> westwoodwilshire.com (Alan Cullen) Date: Tue Oct 31 13:20:07 2006 Subject: [Histonet] IHC Supervisor Job Opening- Genzyme Genetics- Los Angeles, CA Message-ID: <00ed01c6fd21$8ba17440$5b2a1c40@winsearch.asp> Hello all, Genzyme Genetics, one of the largest and most successful leaders in Reproductive and Oncology Diagnostic Testing, has a wonderful leadership opportunity that has opened up within the company's headquarters in Marina del Rey, CA (Los Angeles). The company is looking for a very talented individual with a strong immunohistochemistry background to oversee and lead the IHC laboratory. The Supervisor will have oversight for a team of 10 technologists responsible for all IHC staining and lab operations. The ideal candidate will have a strong IHC background, 2+ years of supervisory experience, a BS degree and HT or HTL certification. Should you wish to further discuss this opportunity, please contact me at the number below. Thank you, Alan _______________________________ Alan Cullen Westwood + Wilshire, LLC 310-967-0945 x100 Office 866-865-7401 x100 Toll Free 310-967-0953 Fax alan@westwoodwilshire.com www.westwoodwilshire.com From RebeNoel <@t> aol.com Tue Oct 31 13:52:06 2006 From: RebeNoel <@t> aol.com (RebeNoel@aol.com) Date: Tue Oct 31 13:52:39 2006 Subject: [Histonet] Please unsubscribe me. Message-ID: Please unsubscribe me. Thank you _rebenoel@aol.com_ (mailto:rebenoel@aol.com) From crains <@t> wpmpath.com Tue Oct 31 14:13:07 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Tue Oct 31 14:13:17 2006 Subject: [Histonet] (no subject) Message-ID: <20061031201307.KPHP29912.dukecmmtao03.coxmail.com@dukecmmtao03> Please unsubscribe me. Thank you for all of the good information. Chris Rains From JGREWE <@t> OhioHealth.com Tue Oct 31 15:01:38 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue Oct 31 15:01:49 2006 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 10/31/2006 and will not return until 11/14/2006. I will respond to your message when I return. Thanks, Jackie From tes4 <@t> u.washington.edu Tue Oct 31 16:53:20 2006 From: tes4 <@t> u.washington.edu (Tiffany Pitts) Date: Tue Oct 31 16:48:17 2006 Subject: [Histonet] TRAP staining on undecalcified bone Message-ID: <000f01c6fd3f$5c67c7b0$4b00a8c0@gucancer.local> Hello all, I am trying to do TRAP staining on undecalcified mouse bones cut from Methylmethacrylate embedded blocks. My problem is that I cannot seem to find a protocol that works well and/or is on the market any more. Does anyone know of a protocol I can use or (even better) a kit? Our in-house protocol is light on details and I'm not sure if I'm doing it right. Thank you Tiffany Pitts Department of Urology University of Washington Seattle, WA From WWmn916 <@t> aol.com Tue Oct 31 21:37:09 2006 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Tue Oct 31 21:37:18 2006 Subject: [Histonet] Ink, slides, cassettes Message-ID: Hello everyone, Two questions: I know this question comes up every now and then, but has anyone else been having problems with cassettes not holding the print from a Surgipath cassette printer? Cassettes, and not all colors, are fading drastically after coming off the processor and in some cases looks like runny, blobs of ink. We also seem to be having some problems with handwritten slides fading drastically after staining. We've used Securline pens for a long time and now they aren't doing so well on slides, especially the Superfrost Plus slides. The Marketlab pens seem to do a little better with legibility but smear badly if your fingers are slightly wet....and they don't last long. Thanks, Deb King Sacramento, CA