[Histonet] Hexane freezing Method for Cryosectioning

[pmarcum <@t> vet.upenn.edu]

Good Afternoon,

I have been asked to look into using hexane in rapid freezing of tissues
(primarily non-decalcified bone).  I am looking it up with several other
searches and wondered if anyone is currently using this technique.  Is it
better or safer than isopentane with liquid nitrogen?  I know the issues with
isopentane and explosions so this partly a safety questions also.

Thanks for the help in advance.

Pam Marcum
UPENN Vet School
New Bolton Center