[Histonet] processing poyester filters

Andrea Grantham algranth <@t> u.arizona.edu
Tue Nov 7 17:31:36 CST 2006


One of my past projects involved processing and sectioning cells grown on 
corning transwell inserts. Could they be similar to the filters that you 
are talking about?
For these inserts, I processed using our old processor - an old Tissue Tek 
dip n'dunker for one hour in each station including 2 paraffins (our 
regular overnite processing schedule). They cut and stained great.
I didn't fix them myself but the inserts were flooded with formalin - 10% 
(as recommended by corning) and allowed to fix for one hour at RT. After 
fixation they replaced the formalin with 70% ETOH and brought them here to 
the lab for processing.

Andi Grantham



At 03:22 PM 11/7/2006 -0600, you wrote:
>We have a researcher who is growing porcine intestinal epithelial cells
>on snapwell polyester filters.  He wants us to stain with the PAS and
>alcian blue.  Our first attempt did not work.  The filters were fixed in
>4% paraformeldehyde for 10 minutes and processed in our regular
>overnight processing schedule. Our first thought is that we processed
>way too long.  What type of processing schedule should we be using and
>how long should we be fixing these filters?
>
>
>
>Margaret Perry HT (ASCP)
>
>IHC Lab Manager Veterinary Science
>
>Animal Disease Research and Diagnostic Lab
>
>South Dakota State University
>
>Box 2175 North Campus Drive
>
>Brookings SD 57007
>
>
>
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>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
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