[Histonet] Thin Paraffin Sections

[Gayle Callis]

Lin,

0.5 um is very thin for paraffin medium.  The thinnest paraffin section we 
have cut is 1 um using Accuedge disposable blades, either high or low 
profile.   When we need the thinner 0.5 um sections, we use glycol 
methacrylate plastic embedding and section with glass knives.  If you 
decide to cut thin paraffin, a harder paraffin often is better - there are 
several on the market.

At 10:18 AM 11/6/2006, you wrote:
>Hi Histologists.
>Someone came by our lab and asked about very thin sections in paraffin,
>0.5 um to be exact.
>Has anyone any  experience or information on this.
>Thank you very much.
>
>Lin S. Bustamante, B.Sc.; HT(ASCP)
>Histology Lab
>Dept. of Veterinary Anatomy and Public Health
>Texas A&M University
>College Station, TX 77843-4458
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)